LOCUS       DACDUU010000001        91743 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.001, whole genome shotgun sequence.
ACCESSION   DACDUU010000001 DACDUU010000000
VERSION     DACDUU010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 91743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 91743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..91743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.001"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            18..1655
                     /locus_tag="IWL30_000001"
     CDS             18..1655
                     /locus_tag="IWL30_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP9704806.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(1800..2540)
                     /gene="truA"
                     /locus_tag="IWL30_000002"
     CDS             complement(1800..2540)
                     /gene="truA"
                     /locus_tag="IWL30_000002"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP9704807.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(2731..3366)
                     /locus_tag="IWL30_000003"
     CDS             complement(2731..3366)
                     /locus_tag="IWL30_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP9704808.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3513..4754)
                     /gene="sstT"
                     /locus_tag="IWL30_000004"
     CDS             complement(3513..4754)
                     /gene="sstT"
                     /locus_tag="IWL30_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAP9704809.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            4952..5950
                     /locus_tag="IWL30_000005"
     CDS             4952..5950
                     /locus_tag="IWL30_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9704810.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            5963..7981
                     /locus_tag="IWL30_000006"
     CDS             5963..7981
                     /locus_tag="IWL30_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAP9704811.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(8041..8757)
                     /locus_tag="IWL30_000007"
     CDS             complement(8041..8757)
                     /locus_tag="IWL30_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP9704812.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(8984..9526)
                     /locus_tag="IWL30_000008"
     CDS             complement(8984..9526)
                     /locus_tag="IWL30_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9704813.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(9531..9758)
                     /locus_tag="IWL30_000009"
     CDS             complement(9531..9758)
                     /locus_tag="IWL30_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAP9704814.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(9837..11717)
                     /locus_tag="IWL30_000010"
     CDS             complement(9837..11717)
                     /locus_tag="IWL30_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP9704815.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(11906..12520)
                     /locus_tag="IWL30_000011"
     CDS             complement(11906..12520)
                     /locus_tag="IWL30_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9704816.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            12662..13222
                     /locus_tag="IWL30_000012"
     CDS             12662..13222
                     /locus_tag="IWL30_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9704817.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(13527..13841)
                     /locus_tag="IWL30_000013"
                     /pseudo
     CDS             complement(13527..13841)
                     /locus_tag="IWL30_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(14095..15730)
                     /locus_tag="IWL30_000014"
                     /pseudo
     CDS             complement(14095..15730)
                     /locus_tag="IWL30_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16053..16532)
                     /gene="rlmH"
                     /locus_tag="IWL30_000015"
     CDS             complement(16053..16532)
                     /gene="rlmH"
                     /locus_tag="IWL30_000015"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAP9704818.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            16960..18249
                     /locus_tag="IWL30_000016"
     CDS             16960..18249
                     /locus_tag="IWL30_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP9704819.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(18288..18893)
                     /locus_tag="IWL30_000017"
     CDS             complement(18288..18893)
                     /locus_tag="IWL30_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAP9704820.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(19098..19562)
                     /locus_tag="IWL30_000018"
     CDS             complement(19098..19562)
                     /locus_tag="IWL30_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9704821.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(19584..19904)
                     /locus_tag="IWL30_000019"
     CDS             complement(19584..19904)
                     /locus_tag="IWL30_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP9704822.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(20048..21127)
                     /gene="pepA"
                     /locus_tag="IWL30_000020"
     CDS             complement(20048..21127)
                     /gene="pepA"
                     /locus_tag="IWL30_000020"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAP9704823.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            21327..21677
                     /locus_tag="IWL30_000021"
     CDS             21327..21677
                     /locus_tag="IWL30_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP9704824.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            21900..22847
                     /locus_tag="IWL30_000022"
     CDS             21900..22847
                     /locus_tag="IWL30_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9704825.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(22904..24322)
                     /gene="pepV"
                     /locus_tag="IWL30_000023"
     CDS             complement(22904..24322)
                     /gene="pepV"
                     /locus_tag="IWL30_000023"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAP9704826.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            24471..25316
                     /locus_tag="IWL30_000024"
     CDS             24471..25316
                     /locus_tag="IWL30_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAP9704827.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            25320..25760
                     /locus_tag="IWL30_000025"
     CDS             25320..25760
                     /locus_tag="IWL30_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAP9704828.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            25810..26004
                     /locus_tag="IWL30_000026"
     CDS             25810..26004
                     /locus_tag="IWL30_000026"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9704829.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            26140..26826
                     /locus_tag="IWL30_000027"
     CDS             26140..26826
                     /locus_tag="IWL30_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9704830.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(27234..27923)
                     /locus_tag="IWL30_000028"
     CDS             complement(27234..27923)
                     /locus_tag="IWL30_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9704831.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(27898..30027)
                     /locus_tag="IWL30_000029"
     CDS             complement(27898..30027)
                     /locus_tag="IWL30_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAP9704832.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(30075..31412)
                     /locus_tag="IWL30_000030"
     CDS             complement(30075..31412)
                     /locus_tag="IWL30_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP9704833.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(31664..32023)
                     /gene="rplT"
                     /locus_tag="IWL30_000031"
     CDS             complement(31664..32023)
                     /gene="rplT"
                     /locus_tag="IWL30_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAP9704834.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(32076..32276)
                     /gene="rpmI"
                     /locus_tag="IWL30_000032"
     CDS             complement(32076..32276)
                     /gene="rpmI"
                     /locus_tag="IWL30_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAP9704835.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(32348..32872)
                     /gene="infC"
                     /locus_tag="IWL30_000033"
     CDS             complement(32348..32872)
                     /gene="infC"
                     /locus_tag="IWL30_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAP9704836.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            33192..33824
                     /locus_tag="IWL30_000034"
     CDS             33192..33824
                     /locus_tag="IWL30_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP9704837.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(33826..34731)
                     /locus_tag="IWL30_000035"
     CDS             complement(33826..34731)
                     /locus_tag="IWL30_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP9704838.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(34816..35469)
                     /locus_tag="IWL30_000036"
     CDS             complement(34816..35469)
                     /locus_tag="IWL30_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP9704839.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            35791..36735
                     /gene="mvk"
                     /locus_tag="IWL30_000037"
     CDS             35791..36735
                     /gene="mvk"
                     /locus_tag="IWL30_000037"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAP9704840.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            36728..37705
                     /gene="mvaD"
                     /locus_tag="IWL30_000038"
     CDS             36728..37705
                     /gene="mvaD"
                     /locus_tag="IWL30_000038"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAP9704841.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            37718..38803
                     /locus_tag="IWL30_000039"
     CDS             37718..38803
                     /locus_tag="IWL30_000039"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAP9704842.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            38800..39864
                     /locus_tag="IWL30_000040"
     CDS             38800..39864
                     /locus_tag="IWL30_000040"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAP9704843.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(40113..40916)
                     /locus_tag="IWL30_000041"
     CDS             complement(40113..40916)
                     /locus_tag="IWL30_000041"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAP9704844.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(40936..41778)
                     /gene="kduI"
                     /locus_tag="IWL30_000042"
     CDS             complement(40936..41778)
                     /gene="kduI"
                     /locus_tag="IWL30_000042"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAP9704845.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(41794..42801)
                     /locus_tag="IWL30_000043"
     CDS             complement(41794..42801)
                     /locus_tag="IWL30_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP9704846.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(42808..43452)
                     /locus_tag="IWL30_000044"
     CDS             complement(42808..43452)
                     /locus_tag="IWL30_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP9704847.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(43552..44310)
                     /locus_tag="IWL30_000045"
     CDS             complement(43552..44310)
                     /locus_tag="IWL30_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAP9704848.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(44437..45588)
                     /gene="fucO"
                     /locus_tag="IWL30_000046"
     CDS             complement(44437..45588)
                     /gene="fucO"
                     /locus_tag="IWL30_000046"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAP9704849.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIVHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(45670..45981)
                     /gene="rhaM"
                     /locus_tag="IWL30_000047"
     CDS             complement(45670..45981)
                     /gene="rhaM"
                     /locus_tag="IWL30_000047"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAP9704850.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(45983..46828)
                     /gene="rhaD"
                     /locus_tag="IWL30_000048"
     CDS             complement(45983..46828)
                     /gene="rhaD"
                     /locus_tag="IWL30_000048"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAP9704851.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(46842..48098)
                     /gene="rhaA"
                     /locus_tag="IWL30_000049"
     CDS             complement(46842..48098)
                     /gene="rhaA"
                     /locus_tag="IWL30_000049"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAP9704852.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(48095..49555)
                     /gene="rhaB"
                     /locus_tag="IWL30_000050"
     CDS             complement(48095..49555)
                     /gene="rhaB"
                     /locus_tag="IWL30_000050"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAP9704853.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            49708..50577
                     /locus_tag="IWL30_000051"
     CDS             49708..50577
                     /locus_tag="IWL30_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9704854.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(50703..51602)
                     /locus_tag="IWL30_000052"
     CDS             complement(50703..51602)
                     /locus_tag="IWL30_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9704855.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(51767..52591)
                     /locus_tag="IWL30_000053"
     CDS             complement(51767..52591)
                     /locus_tag="IWL30_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9704856.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(52602..53897)
                     /locus_tag="IWL30_000054"
     CDS             complement(52602..53897)
                     /locus_tag="IWL30_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAP9704857.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(53913..54392)
                     /locus_tag="IWL30_000055"
     CDS             complement(53913..54392)
                     /locus_tag="IWL30_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP9704858.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(54398..55426)
                     /locus_tag="IWL30_000056"
     CDS             complement(54398..55426)
                     /locus_tag="IWL30_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAP9704859.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(55443..56549)
                     /locus_tag="IWL30_000057"
     CDS             complement(55443..56549)
                     /locus_tag="IWL30_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP9704860.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(56778..59375)
                     /gene="adhE"
                     /locus_tag="IWL30_000058"
                     /gene_synonym="adhC"
     CDS             complement(56778..59375)
                     /gene="adhE"
                     /locus_tag="IWL30_000058"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAP9704861.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(59680..60060)
                     /gene="yajC"
                     /locus_tag="IWL30_000059"
     CDS             complement(59680..60060)
                     /gene="yajC"
                     /locus_tag="IWL30_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAP9704862.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(60157..61302)
                     /gene="tgt"
                     /locus_tag="IWL30_000060"
     CDS             complement(60157..61302)
                     /gene="tgt"
                     /locus_tag="IWL30_000060"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAP9704863.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            61520..62218
                     /locus_tag="IWL30_000061"
     CDS             61520..62218
                     /locus_tag="IWL30_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP9704864.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(62386..65025)
                     /locus_tag="IWL30_000062"
     CDS             complement(62386..65025)
                     /locus_tag="IWL30_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9704865.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEI
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(65644..66675)
                     /gene="queA"
                     /locus_tag="IWL30_000063"
     CDS             complement(65644..66675)
                     /gene="queA"
                     /locus_tag="IWL30_000063"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAP9704866.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            66868..68043
                     /locus_tag="IWL30_000064"
     CDS             66868..68043
                     /locus_tag="IWL30_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9704867.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            68047..68688
                     /locus_tag="IWL30_000065"
     CDS             68047..68688
                     /locus_tag="IWL30_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9704868.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            68685..69602
                     /locus_tag="IWL30_000066"
     CDS             68685..69602
                     /locus_tag="IWL30_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9704869.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            69599..70270
                     /locus_tag="IWL30_000067"
     CDS             69599..70270
                     /locus_tag="IWL30_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9704870.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(70811..71299)
                     /locus_tag="IWL30_000068"
     CDS             complement(70811..71299)
                     /locus_tag="IWL30_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAP9704871.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(71500..71766)
                     /locus_tag="IWL30_000069"
     CDS             complement(71500..71766)
                     /locus_tag="IWL30_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP9704872.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(71766..72029)
                     /locus_tag="IWL30_000070"
     CDS             complement(71766..72029)
                     /locus_tag="IWL30_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9704873.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(72022..72294)
                     /locus_tag="IWL30_000071"
     CDS             complement(72022..72294)
                     /locus_tag="IWL30_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9704874.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            72505..72690
                     /locus_tag="IWL30_000072"
     CDS             72505..72690
                     /locus_tag="IWL30_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9704875.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(72832..74655)
                     /locus_tag="IWL30_000073"
     CDS             complement(72832..74655)
                     /locus_tag="IWL30_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAP9704876.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(75253..77958)
                     /locus_tag="IWL30_000074"
     CDS             complement(75253..77958)
                     /locus_tag="IWL30_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAP9704877.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(78264..79211)
                     /locus_tag="IWL30_000075"
     CDS             complement(78264..79211)
                     /locus_tag="IWL30_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAP9704878.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            79401..79670
                     /gene="rpsN"
                     /locus_tag="IWL30_000076"
     CDS             79401..79670
                     /gene="rpsN"
                     /locus_tag="IWL30_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP9704879.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(79846..80208)
                     /locus_tag="IWL30_000077"
     CDS             complement(79846..80208)
                     /locus_tag="IWL30_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP9704880.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(80219..81340)
                     /locus_tag="IWL30_000078"
     CDS             complement(80219..81340)
                     /locus_tag="IWL30_000078"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAP9704881.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(81356..81706)
                     /locus_tag="IWL30_000079"
     CDS             complement(81356..81706)
                     /locus_tag="IWL30_000079"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAP9704882.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(81836..83347)
                     /locus_tag="IWL30_000080"
     CDS             complement(81836..83347)
                     /locus_tag="IWL30_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP9704883.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(83669..85021)
                     /locus_tag="IWL30_000081"
     CDS             complement(83669..85021)
                     /locus_tag="IWL30_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAP9704884.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(85174..86250)
                     /locus_tag="IWL30_000082"
     CDS             complement(85174..86250)
                     /locus_tag="IWL30_000082"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAP9704885.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            86446..88191
                     /locus_tag="IWL30_000083"
     CDS             86446..88191
                     /locus_tag="IWL30_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAP9704886.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(88471..88908)
                     /locus_tag="IWL30_000084"
     CDS             complement(88471..88908)
                     /locus_tag="IWL30_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAP9704887.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(88892..90265)
                     /locus_tag="IWL30_000085"
     CDS             complement(88892..90265)
                     /locus_tag="IWL30_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9704888.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(90587..91075)
                     /locus_tag="IWL30_000086"
     CDS             complement(90587..91075)
                     /locus_tag="IWL30_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9704889.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(91097..91699)
                     /locus_tag="IWL30_000087"
     CDS             complement(91097..91699)
                     /locus_tag="IWL30_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP9704890.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        26767 a        19275 c        16403 g        29298 t
ORIGIN      
        1 ttaaatagga gaaaaaaatg aaaaaaaaca aaaaccaaat aatagatatc atttttatgt
       61 tatttctaag ttttgcttac gtcatccctc tattcatgtc taagggaatc tatcatagtg
      121 tcaatcagga tacttacttt catttatctc gaatcatagg actagataat gtatggagtt
      181 ctccagtaaa cttcaataac ttcgatcatc atggaacaat gatgaatatt ttttatccat
      241 ggcttactct ttatccagct tttttatttt ataaaatgat gggaaatctt gttttaagtt
      301 ataatatcta ctattttttt atcacttttt taacaatggt cgtttcttat ttttctatga
      361 aacaaataaa aaacaatcgt tatatttcgc tgttattttc gattatttat acattttccg
      421 cctatcgtgc cattgacata ttccatcgtg cttcattggg agaagctgtc gctttaacat
      481 ttttgccttt gatactaatg gggtgttacg aaatttatat tagagattac caaaaatggt
      541 actggttgtc tattggcatg acgctagtag tgtatactca tttactttct gtcgctatgg
      601 tttctgtatt tattggagga acgttgtttc tttcttttta tttttgggac caaaaaatcg
      661 ctagactctt aagtctttta aaagctaccg tcctcacctt ttttttaagt gcgggattct
      721 taattccttt tattcaacaa tctagagcac aagaattgaa agtcccactg ggaaaagaat
      781 taagtggtat ggctcctagt gatatgctga cacatatttt aaataataac tataacaact
      841 atactattgg attattctta tttctcggat tgattggtgc ttttattttt ataaaaaaat
      901 taactgctga tgatttattt attttctttc ttggtgtatt tgttcttttt tgttcaacca
      961 atttatttcc ttggcaactt tttaaccata caccagttaa gagtctacaa tttgtctggc
     1021 ggttaaatgg tttttcttct ttatttatag cctatacgat gagtatagtg atctattata
     1081 ttttttctac taaaaataat tcatggaaaa taatggtctt tacgtttctt gcactaatat
     1141 tgcatctctc aggagtaagc aattctatcc atgctaacaa agattcatta acacttatag
     1201 cacctgaaga cgctgttgct atagctgaaa attacaatca tactgattat gcaaacaaag
     1261 agtcaatcta tcatcctgat atgattaata atgatcaata tttactaggt aaccaggaaa
     1321 taaatccaac aactcatttt atgagtaaca aacttacaat tgaattagat aattcgaata
     1381 ataaaaatac tgtcttgacg acacctattt atcgttataa agggcaagtg gcttctatta
     1441 atggcaaact agtacaaaca aaattatcta agtttggcac aactgaatta actattcctc
     1501 caggtataaa taaagtcgtt attacttatc agtacacaaa actagcaatt gcttcaagat
     1561 atttatctat cgttactttg attttatttt tactttatcg atttactttt tctaaacaaa
     1621 aacaaccgag aagagaagta caacattctc actagatttt tatttaagaa agacttttta
     1681 gctgtacaca taaacaggct aaaaagtctt tttttatatt tctttaattt gcttcataca
     1741 ttatttaaca tcgatattta tactgtgaaa catgcgtttc atccattatc aaagtatttt
     1801 tatcgataat aaacttcatc caaaaacaga ccttgtgcag ggacggtttc tcctgcatgt
     1861 tgccgcactt tgctttcaaa tatttcatcg attgtttcta tcggcatggc acctgtgccg
     1921 atttctagta atgtccccat gataatccgc accattttat ataagaatcc gtctccgaca
     1981 aaagtaaaat gaagcatttc tccctttcgt tcgatagata attcatcaat cgtacgcact
     2041 gtggattttt ttgatttttt caatgcagaa aaaccaatga aatcatgtgt acccactaat
     2101 ttttcgcaag ctgcattcag cttgtccata tccaactctt gagggaaata aaagctgtgg
     2161 tttcgctcaa atgctgaagg aatcacgttg ttccaaacat aatagctgta ttttttgccc
     2221 accgcattat atcttgaatg gaagcgctca ggcatttctt cgattctttt gatgacaata
     2281 tcccgcggaa ggtaacgatt cataaagtcg atcatttctg ccagtccaat ttctgtgttt
     2341 gttttaaaat ttgctacttg tcctcgcgca tgggtgccag catctgttct tcctgatcca
     2401 ataatttcta tctcttcatt tgtcatctga tgcaatactt tttcgatttt tccttgaatg
     2461 gttttatcgt catctccaag tctttgccat cctgaatatc tttttccgtc atattcgatt
     2521 gtcaacttga tgtttctcat attttagtgg ctcctctgtc ttccatttgg tcttctctct
     2581 acctttttaa gattgtagca caaaatatag ctccttgctt ctctaagaca gaagctatac
     2641 aagaaaactg tggaaatata tatttcagaa aaacaaaaaa cagccaccgg atgaagtatc
     2701 cgctgactgt tcaatttgac ggctcaagtt ttactgtttg agatgcaaga tactcaacca
     2761 ggcaatgggc acataattaa agaaaaaact gattctttgg caaaaaccta ttttattcag
     2821 taatggccaa gaatttatag aaggaattct tgccagtcca taaatcgccg caaaaagcaa
     2881 actgatcaca gacagtccaa ggtataagtg gcttacgtcc atttgcccat tttttcgata
     2941 gatcaagaaa agaagaactg gaaacaataa aaaaccactg taacctaaac ctgatccgat
     3001 attatggacc catgtcgaga cattccacga agattcatgg gtattgatac tgaacaaacc
     3061 tgtaaagata caatcaccta taccatagaa cgcgatggct gctgtaacta gggtagctaa
     3121 cacacgagat gtactaacaa atgcagcata caatgcaggc aatgacagga tgaaaaagat
     3181 ccctgaaacc acagaccaaa taagaaacgc ttttcttaca ggactggaaa cctctccaaa
     3241 tacactgatc acgctcttca tttgatctaa ttcaggataa tacaatccta atacgtacgg
     3301 agtcaaaaaa tcactcagca cgcctaataa taaaaagaaa aaaccaaatt tacgtaaaaa
     3361 atccatgata cgcctccaat aacaattacc tatgctttta attgttatta taaacgactt
     3421 tatcttgatt taacagaaac ctctatttac acgaacatct atataactca ttaaaaactc
     3481 gaatcatttt aaaaatacta aatatctaac gttcaacgtt cacttaatcc atctaaacaa
     3541 tctttcaatt tgattttttc cccacttctt ttacgcgcgc ctagctctcc tattgcagta
     3601 aacaacagat cacttgacga atttaaggca gtttccattg aatcctggat aacaccgata
     3661 ataaatccaa taccgacgat ttgcatcgcg acatcattag agataccaaa caaactacat
     3721 gctaatggaa tcaataacag tgaaccgccc gcaacacctg aagctccaca agcagctaaa
     3781 gcagacaaaa tacataaaag gactttcaaa acaaaaggtg cttcaatccc taaagaatgt
     3841 acagcagtca acgtcatcaa ggaaatggta atcgcggcac cgcccatatt gatcgtagca
     3901 cctaacggga tcgatatgga ataagactct tttgtcaatt tcaatctttc agccaacatc
     3961 atgtttatcg ggatatttgc ggcagaactt cgggtaaaga aagcgggaag tgcgctttct
     4021 ttcaaacaaa agaaaatcaa tggataggga ttttctttaa taaacaaaaa cgtgaggaat
     4081 gggtacacaa ctaatgcgac aaacagcatc gtaccgatca aaacaagtaa aagctgcaga
     4141 tactctgcca atccagcgat tccagtcgta gcaattgatt gataaaccaa tcctaaaatc
     4201 cctatcggag caaatgcaat aatcacttgt acgacttgtg acaacgctat agatatctga
     4261 tccaccaact cttttgtcga ttctgaacgt gctcgaaaag ctagtcccaa caatacagcc
     4321 cagaataaga cgccaaggta attcgcatca ataagagatt gaacaggatt ttgtgtgaca
     4381 ttcgtcaaca tagtagataa aacactttcc aaactttgcg gtgctttttc agatactgtt
     4441 tcttgtaaag taataggaac agtaaaaatt gaacttacaa taactgctac aagtgcagaa
     4501 agcaatgttc ccactaaata taagatcaaa ataggcttca cgaatgtctt attcccaatt
     4561 ttatgcttgg caattgacgc catcgtcaag aaaaaaacca gaataggtgc gattgccttc
     4621 aagcaactga caaacaacgt tcccaatatt gaaataaatg tccatgtggg gaaaaagata
     4681 ccaaacaata ttccgactaa aataccaaca acaatacgtt gaactaacga catatccaaa
     4741 aacttctcta acaaatgacc accctttcac aattttaaca atcattgtat cattttatgc
     4801 aaagaaacta aaggtataat tttctaaaaa ttctaacaat acaaacaacc atttcttgct
     4861 aattttgtac tttttctgtc tacacgttgt tttttgagat attctaagtt aggatgttat
     4921 taatgggcaa cttatcagga aacgttttct tatgtttttg tccaattttt ttgtgaggtg
     4981 tttattcttg aaaaaaataa agaaggagag ttatggcttc ctgacaatct ttgtcgcttg
     5041 tataatgatg gttttacagc gtatattgat ttcagcagtc actattccta catatgcaac
     5101 aaaccctggt cgattctaca tctatacatt tttacaaact tccgttgtta ttggtgcaaa
     5161 tttgatccca ctttacatgg gttaccaata tcaaaaaatc gcccgtttaa aagttttaca
     5221 ttatttaagt cgttttgcct ttatattcct aatagccaca ctagtttgca atatcttttt
     5281 ttacgtacaa gctggcagct tgaatattcg tgattattgg cttatcctaa cacctatcag
     5341 tcagaattac tttacttatg ctgtttcttg tactttgctt ttttgtagta ttccttatac
     5401 agttggattt ttaaataaat tatcgaaaac aacaattaaa tatctactat tatttttaac
     5461 aattggatta gttggctgct ctactctatt taataaagac ccgtttgcat tacagaatgg
     5521 acagagtatt ttatggattt tttatttatt tttaatgggt tatggtttga aagaatggaa
     5581 ctggaaagaa aaggtccgct ataagcttcc tcaagtattt atcagcttga ttgttttatt
     5641 cggtttgatt attttgatga cacaaatttc actcatcatc agaggaaata cagatacagc
     5701 gctgaggttt tctaaaccat attctctctt cgcaatgtat tatactttct cttcgttttt
     5761 atttttagaa atgctatctg ataaattagg tataaaagga agaggaaatt ttcttgctgt
     5821 atttttgatt gtcactcaag ttatatgtaa tttcccttta gtttcttatc gcgtatctac
     5881 atttatgaaa cgtgaatttc caaactcagg gaaggcatgg ctgataaata tcgggcagtt
     5941 ttttatttga tatttattag cagtgtccct cctagttatc tttttatttt atatacaaaa
     6001 aatccgtttc attcatcgtt tcatcgaata cttcgctttt gattcgattg aacaattaac
     6061 tcagcgttgg aaaaaaagaa ttcattggct ttttgttcat aaaagttatt ttttagttgc
     6121 agcatttttt tactgcttta ccttcgtgca gattttcgtt ttagaaccaa aagaaggttg
     6181 gaaagaaact atccaggttg ccttaaaaat atttactcag cgacaagcac ccatgatcct
     6241 gacaatcatc attattatgg gatttttctt cttactattg ttgatttcca atcgtttttg
     6301 gtacgccttg actgctacct tgatcattaa cttactgctc actatttcga cagtaatcaa
     6361 aatggaaatg cgggaagagc ctgtctttcc ttcagattta aaaatgctga ccggattgtc
     6421 agaactatta tcgatggtta gcccagtctt attaatcgtg ggcggattga tccttctcct
     6481 attattgatt actagtatta ttgtacaacg tagactacaa aaacagtatt ccttgaagat
     6541 tcattggaaa cgtcgattta tcagtatcgc tgtattatta ggatgttttt cgggtgtctt
     6601 tttcattaat cacaaaaatt cgccttcttt cttgctgttt aatttattta aagtaaacaa
     6661 gactttcttt aaccagcaag gcgccgtaaa agataacgga ccagtcattc aattcctaaa
     6721 caatatcgac attaaaatca tggaagaacc tgctggctat tcgaaagaaa aaattaatgg
     6781 aataatgaaa aagtacaaca caaaggcaga tgaaatcaac caaacaagaa gtgactggtt
     6841 aaacaatcag acgatcgttc tcaatttaag tgaaagcttc agtgatcctg cacgtgtccc
     6901 taatctgaca gtacctacag accctattcc ttacatcaaa agcattataa cagaaggaac
     6961 aggaggattg atgttatccg tcggttatgg tggcggaaca gctaacatgg aatgggaagg
     7021 actgacaggc ttgagtatca gtaacctgtc tgcttcgtta gttacacctt atacccagct
     7081 agtggagcgt cagaaaataa gtccaaatat taccgatcta ttcgatgaaa aaatagccat
     7141 ccatcctttt acagctgctc tttatcgcag aaaacaagtg tttgataaat ttggttttga
     7201 caggttctat tatttagaca gtccggataa attaacttat acggataaaa tagaacaaag
     7261 cccacgcata tcggatgatt cagcatacaa agaaaccttg aaagctttac aaagcaatac
     7321 ggaaacgtca caattcatcc aattatccac tatgcaaaac catatgcctt atgatgatta
     7381 ctatagtgaa ttgaactaca ctgctgaagg aactgctgtc attcctaata gaagaaaaga
     7441 attacaaact tatatgcaag gccttcacta taccgataca gcagtaaaaa aattcatcca
     7501 agaaatcgac aaaatcgata aaccgattac ctttgttttt tatggtgacc atttacccgc
     7561 aatctatagt gggaatgatt cgaaaaaata tggattagaa cagcaccaga cagattattt
     7621 catttataac aacaagtata gtcgtgaaca agcagctacg ttaaataaag aagttgttgc
     7681 tcctaataac tttggtgcta tggctcttga gcatgcgaat atcaaagtaa ctccttacta
     7741 tgcgttgttg acagaagtat ctaatcatct acctgcatta acaattgatc ctacagaaag
     7801 cttgtctaat cgttttaatg gaaaacaggt ctttgttaca gaggatagtc aaattatcca
     7861 agaaaaaaat ttaactgaga aacaaaaaga aattctagaa gattatcgtc tgatacaata
     7921 tgatcttgtt gcaggtaacc agtacagtgc aacatgggcg acacaaaaaa taaaagacta
     7981 acaaataaac tgagcaaggg gttgtgataa aagacatgtc acaacccctt gtttatctca
     8041 ttaatttgct tgtccataaa cgatttgatt aaagccattt ttcaattcat tattcgctgt
     8101 ctcgttgaag taaagataat tatcgggatt cccctgattt tgttgtgtca ccaatacttt
     8161 tggctgattt tgctgaaacc cgaatagata gacgtgcttt ttcagatatg gttctgtctc
     8221 tgcatcggaa tagacggctg tgatttgata gtctgcttga cctgtcccac tttctgacca
     8281 ttctgcagaa acaggcactc caccaatagc catcttccac tccccgttca aaatagcttg
     8341 agggacttta agaccatata agtccacact catttgattc gtataacttt tgtattgttg
     8401 tcccattgtt tggccccatt tggtcataaa tgcctctaat tgtgcggatt tgtctgcatt
     8461 ccacagtgtt tgttcaactt gttcgctgct ttctgaagag gaagatgaag cctctacaga
     8521 agaacttgaa gtagatgttt gagttgtttc agtcgttttg gaagactctg acgaagtaga
     8581 ggtatctgct gtttctgtac ttgctgcagt tgacttgctc tctgtgctag ttgttgtagt
     8641 agttgcaact gttgctttcg ctgtttcttt tgttgattct tttttcgatt cattcccgtt
     8701 ggtacaacca gctaacaaaa aagctagacc aattgttact aaaatccttt ttttcaatta
     8761 aaaaactcct ttcgattact cccacttctt tattctatca aataagcaat gtgattttat
     8821 tgatttttcg tattttctca tcaatctttc aaatcttcta aaaactgaga agcacccgct
     8881 gatgttgtct gtaatacatc agcgggtgct tcttttattt taatcttcgt gattttccaa
     8941 tttacctcac gctttatatt tgtatttgaa gaaacataga cttttagtca tcatcgtctt
     9001 cctcatccaa atcttgtcgg acagtttttc cttggagtct ttgtacttgg tagatgattt
     9061 ctttattgaa acttaaaagt tcaacaagca attgttgcaa gccatatttt tcttcttttt
     9121 cagctaattt gattgcccgt gtgataaaga cattttggaa atcaaaatct tttgcaaagt
     9181 tttccaacat tgttgaggct ggtaaatatt tgttttgacc atgttcgtaa atttttccat
     9241 aacgcatgac ttcttctaaa gtagacggaa cgatttcatt ttcaatcact aactcatgag
     9301 agattttatt gaaccatctc gcttcttgat cagcatattc tgaaaaaagg gtacttaatt
     9361 cttgcgaaga tgtacccaaa acatacaatt gtgcttggat caatttcatt tcttctaatt
     9421 taaaatttgc taatacatgc cccaccatgg cgccagcagt tggtttatga tggtcgatat
     9481 ctgcttgttt tgtttcttga acaaattttt cttccactat attcattgat ttacgcctct
     9541 tttacttttg ctgattggac ttcataacct aatttttcca caactgcagc taattgttca
     9601 gggtggactt catctggatc aaagtttgct ttgattttgc tcgcattaaa caaaactttc
     9661 gtattgctta ccccaggctg ttgagaaacc gcatgttcga ttttttgcat acagcttgga
     9721 caagctagtg tatctaattg cataataatt tttttcatcg tagttcttcc ttcctttttc
     9781 ttgatagtct tattctagat tacagcttcc ataaaaaaat tgattcttat caagttttac
     9841 gatttttctt ctttgctttc gtgtccaaaa tacaataaac gcatcgcgtt aaagataacg
     9901 atcaagatac tagcttcgtg aacgaacatc ccactagcca tgtaaataaa cccggcaaat
     9961 aagccgttga gtaaaaataa gacgacacca atggcaatca ctacattttc acgggtattt
    10021 aaaacagttt tcttagccaa gcggtaggca tgaaccaacg attcaaaatt agattgcatc
    10081 aagacaacgt cactcgtttc gatagcaaca tccgttcctg atcccatagc aatcccgata
    10141 tcagccgcag ccaaagaggg agaatcattg attccatctc caataaaagc aacgcgcaat
    10201 ccttgttctt ggtatttttt cacaaattga accttttcgt ctggtaataa ttcagcatgg
    10261 acttcatcaa tgcctaacat atcagctaca taatcagcag tcatctgatt atcacctgtc
    10321 agcatcacta actgttttgc tcctgcttgt ttcaacttag ctaattgttc agcgacttct
    10381 ggtcgaatca catctgacac acctatgatt tgagtgattt ttttgtcgat tgctacaata
    10441 atgaccgagc tgcctgtttg ttgtaattga tgtaaatctt gtacttgctt tggtgtgaga
    10501 gaaagtttgt gagcggcaat caatttggtg ttgcccgcac aaactgtatg accatcaatc
    10561 tcagctgtga ttccttgtcc cttgattgtc tgattatctt tgactgctaa ttgttgaaaa
    10621 tcaatgtctt tctcctgagc ataatggatc acggcgcgcc ccagaggatg atcggataac
    10681 gtttctaatc gagccactaa agctaagctg tccccttcat cctctgtata gttcttgata
    10741 gtggatacag cagtagttcc tttggtcaat gtaccagttt tatcaaagac aaaagtatcg
    10801 attttggaaa aagtatccat tgcttcaccg cctttgacta agatgccccg tttcgctcca
    10861 ttaccgattc ctgctacatt cgaaacaggc gcaccaatga ctaaagcgcc aggacaacca
    10921 agaaccaaaa cagtgattgc taaacgaaaa tcacgactaa tcagaccaac aaccaacgca
    10981 ataagtaaaa cggttggagt ataatactga gaaaatcgat caatgaattt ttccgcatgg
    11041 gatttggtat cttgtgcttc ttctactaat tcgataattt tggcaaaggt cgtatcatct
    11101 ccgaccttag ttgcttcaat cgtcacgtag ccatctgcta aaatcgttcc agagaagact
    11161 tcatctccac tgattttgac gacttgtcgt gattcaccag tcactgcggc ttcatccgca
    11221 taccctttcc cgtcaatgac tcgtccgtcc acaggaatag ctccaccagt ttttactaag
    11281 acgatatcgc cttcttccac gtcatcgatt tccacttctt cagtcgttcc atcttcttgg
    11341 acaacactag cagtagatgg ggccatctcg gtcaaagcct tgattgactc ccgcgttttg
    11401 gtcaatgttt tttgttccag atagctacca aacaaaaaca agaatgtaac gatagctgac
    11461 tccttgtatt ctccgatcat aaaggcacca agcacagcaa tagtaacgag taaatcaatt
    11521 gagataactt tgactttcac tgcctgatac gcatggatca taataggtat gaccgcaata
    11581 atagaagcta tggctagagc aatgttatag aacaagacaa aaccagtaaa ttttccgata
    11641 aaaccaaaaa taatcaacac agctgataag gcggtgatat gatttcgatg ctcttggatc
    11701 cacttttgta atttcatctt tctccctctt ttcctttcat tcttacgaga ctaagtatag
    11761 atacttatcc atcataaaaa attgaccact atcaagttta gtcaaaaagg cggttttctt
    11821 ttggtagaat acactacaaa aaatagttag caagaagact cactaaaagt gatcttgcca
    11881 actattgtcg tttctatcga aattttcatt gaatgatttt cctgtacagc atacatcgga
    11941 gatctatttc tttatctaag atttttatct tgctaatttt ttcctttagt ctttcaaatc
    12001 cacgtcgttc atagaattga tacgtacagc cactatctgt aaaaaggaat atttcttttc
    12061 ctttctctct tttttcaaat tctgttagca acttggtacc gatgcctttt gttttcaaat
    12121 caggatttgc agctaaaaaa atgatctctc catctggcga atgcttcttt aaatatgctg
    12181 caagcatttg ttcatttgct gtctcgtaag ctcctttact ttctttagca aatatatttt
    12241 gtaaaaaatc gaatatctta acatatattt gctgtccaaa agaacgatgt ttcttttttt
    12301 ctccttttat ctccgccagc aaaacacctg ccaactcgtc ctctgcatac aaagcaagaa
    12361 tttgagttgc tcgattcaac tccatataca aaaaatatct tccgtaaaga ttcagcagaa
    12421 gttcattttc catatatctc tcaaagttca ttccttcaac agcaaaatag atagcttttt
    12481 tgtaatcttt cttttctaag ggtttaaaag ttagtttcat catttcgcct cctcaacttc
    12541 aatgattgtg ttatacttac attataatag acacagtgtc tactatctat aatcaatcat
    12601 ctataagttt gtcgattaag cgacagcttt gctttgatat gtcggataag gagataggta
    12661 aatgaaaaag acagatagac gcgtcaaaaa aacagaaaaa gctttggctg aaacactatc
    12721 cacattatta gtaaataaaa agattcaagc aatcactata cgcgaactaa cagaaacggc
    12781 agatgtacat cgttcgactt tttatacaca ttataaggat atctatgatc tatatgatca
    12841 attagaaagc aacttttttc gggatctaaa tgaaattctc tcttatgatc cagcccatag
    12901 ctatgaggaa ctatacactc gcttgattga ctatgtttac gcaaatactt ttttatatcg
    12961 cttatttatc ctcaaccatg gtgatatttc gtttcagaag aaagttaaac aagttattga
    13021 agaaaactat ctgaacatat ggctatatga agacaagaaa acgattatca cagaagaaat
    13081 gagattcttt actacgtacc acgtccaagg ttgtctatcc gttctcaatc ggtgggtaga
    13141 aagcaattac aagtactcca aaaaagaatt attagaactc ttacagaaac tgaatgatca
    13201 catagagaaa atcatgcctt gaataaaaac atactaagct tagttaagaa gaaccgtaac
    13261 cttctcgtat aacaactgaa tcttatttag ccaaattgtt tactgaagat tttttgagta
    13321 aaatttctat aattcctact tattgaaaag tagtttgtag tgctgcttct ccctctctaa
    13381 gtgatatatc ttgttgtacc aatcaaattc acctcatcat taataaatta cttttaggct
    13441 aatttattac aacttatagg ttgaagctgt aaccaattta tagtcatatt atatttttat
    13501 catccagttt attgaattct tctcgtttac ctcaattcat tttcaagggg aatcagctca
    13561 tcatctaaca ttttacgcca tgctcgcttc atttcttgtg ggtatttaaa tggtttcgct
    13621 tctggatgtt tggctttata tccttcgtaa tccttactgt tgataattgc tttcatatta
    13681 ggaattggat cttcacctgg catatattgc agattagaag catgtaattt tttctcagaa
    13741 acaaaccact cattggaaaa gacatgaatc aaatcatcac ttacttgtgt aaaatagcga
    13801 cgtttaatga caaagatatc ttcattgcta tctagtgtac cttcatttct ttgtgcttaa
    13861 attcaaagat aattttctca ataaagattt ttgattccag taagattcct ttactccttt
    13921 tacaacatag cgttgtcctt tgttttgaaa catcttcttc ccctctttgc cattaaaaat
    13981 agcccaattc tctataaaga agattaagct tcattattat aatataaagt ttaaatttgg
    14041 tttattactt actttttctt attctctagt aagctaatat tgaaaaaaat gtaatcagat
    14101 cattattttt atatgttgta acttcctgat aagaaaacta attaaaagaa agaggtaact
    14161 acataaacta attaaaaaag ctaacttcat agttggagga taaatggaag aaacttcaat
    14221 gattgtttct ccttgtgttc cttcaatcga cactagtcct attatcaatc tctctttttt
    14281 gtccatttac ttttgcgact gttccccaat aatcaaatac aggaagcgtt attttttgtc
    14341 ctttagattc atgtactgta tagactattt tcgaatccat cacttgataa ggcatagtat
    14401 gccattcctt gccaattctt acttcatgat tctgcaaatg ttctttgtat tgtaatgctt
    14461 ccgtgggtgc atagtcataa agtcctccgg gaaggagtgt cgtgtaacga ataagatttt
    14521 cttgattcat taccaagtga tctctctgag tgacaacttt atgttcatag tttgcttttg
    14581 ttgcagcaga tacagccaac agtaataaaa caattgccgt tatattaact acttttcttt
    14641 gcttagctga tgtgctcttc atgaaaacga acgataaaat caacgagcca aacagtactt
    14701 gaaagcctaa gattcgccaa ggaaattgaa tcatttgaat gggtgtattt tgtaataaag
    14761 accatggaaa aaatttggtg gataagagcc aagagactac cgcactaagg taaatcactt
    14821 tttctttgcg attaaatcct ttaaaaaaga agagtggaca gatcaaagaa ataataccaa
    14881 tgattcctac cgtaaaggtt gtcaggtcat tattcatcgc accaatcaaa ctatccgaga
    14941 catttaatgc ttggtgttgt aaggtcgttt tatctggctc attaatcgat tgaaccatga
    15001 tttgtcggaa catcggatac caaaagtatg ctgttaaaag aatcgtcatt cctactgcct
    15061 ttgacaactc cacaatcaac cggatgcttt ctttttttct tgtttcactt actacaaagc
    15121 cgcctaagac tagtaagcca acggctatta cactcataaa agtggaaaga acatgtgtat
    15181 aaatgagtaa gctcatactg actgctaatg gaattgcaca tggctttttc tcataaatca
    15241 gtgcgtaagc ataatacata actatcggta taaccgtaag tgcaatcgct tcagcaatag
    15301 cagaacgatg ataaatatca atcgtacggt acccataaaa tgtatataaa cagctaaata
    15361 taaaagccgc atcgattctt tttaaaaatt ttagtccata atgaaacatc agcatgatag
    15421 tacaaacatt caatagccaa acatataaga tataactaac gattaaatta ttacttatcc
    15481 aataaaaaat gactgccgga aaaaaggtca agtagggata aaaataattc actccgctcc
    15541 cataatgatt aaacgtcgta aaattgattg gaccagaaaa aattgaagat aaccctttga
    15601 ctctagcaaa atggaaggca atatcccccg atgtagaaat agtatgaaca tttctaaaat
    15661 aattgattga ataaagtcca gcaataattg ctaggagaat ggacaatatt atttttttct
    15721 ttacttgcat tacttaatta tatactcctt tcatggattg ctttatctag tataacttaa
    15781 attttaaata aatcgttatt tatttttact ctcacttaaa aaagcactaa aattataaga
    15841 gagggaggta atggtctgaa caccaagaat tgttccaaca tcgaattgct taactaatga
    15901 gttcaaagct taattatgtg gctcttatat ttaccgagcg tggattgcga tctctttagc
    15961 gaaacaaaca tctttcataa aaaaaacagc catatccgct ttcttggata tgactattta
    16021 ctactacttt actatctttc attcttcttg tctcacttat gataaggcgc ccccgcattg
    16081 atccggaagg cacggtagat ttgctcgaca aggatcagac gcatcaattg atgtggatac
    16141 gtcatttttc caaaagagat ctgagcattg cttcttttca tgacggcttc gctgagtcct
    16201 aaagagccgc cgataacaaa aacaaggtgg ctttttcctt ggatacctaa ttggtcgatt
    16261 tgtttagcaa aagattcact tgtaggattt ttcccttcaa ttgctaaggc atagacgtat
    16321 tcttgttctt tgattttcgc taagatccgt tccccttctt tttcttttac ttgtctcatt
    16381 tgcgcttcgc ttaaattctc cggggctttt tcgtcaggaa cttcgatcaa ttcgattttt
    16441 gcataggcat tcagccgttt gacatactca ttgatacctt ggatcaggta tttttctttt
    16501 aattttccta ctgagataat tttgatattc atgttttctc tcctttggtc ttcatgctca
    16561 ctttgtttat tataatcaat tttttcgtct atatgtttat tttggctatt tttgttaaaa
    16621 agatttcccc aacagttatg cacaacatcc acaggtttat ccacagaaaa cagtgttatt
    16681 ttaccttatt tcatcacatt cattaggcaa acatacgttt tataagaatt tatccacttt
    16741 atacacaggc tgtggatatg ttttatccct tatttttcca atcctatccg cgtagtttta
    16801 cacagtatct gtggaaaaag aaaatcaaat attttcatct gaaaacaatg atttttccta
    16861 aagtttttca taaaaagttc acagtctcaa agtagactaa agtcaacaaa taagactata
    16921 ctaacagtag ataaatgaat gtaattggag gaggaacaca tggatagaaa aaatgtgaca
    16981 ccaaagatga aaaaaagtaa aaataatagc ctctggcgta agttaggtct ggggcttgtt
    17041 ggagggatcg ttggaggact actgacagct ggcatttttt atgcagcgat gggcactggc
    17101 aacaatagtt ctactgctac aagcggcaat cagaattctg ccggtgaaac agttgtggaa
    17161 aatgtcaaag tcaatgtaga ttctgatatc accaaagcgg tagataaagt tcaagacgct
    17221 gtcgtttctg ttatcaactt acaaagtcaa aaccaatcaa gcggttttgg cggactattc
    17281 gggcagcaag aagaaagtag cagctcagat gatagtaatt tggaagctta tagtgaagga
    17341 agcggcgtga tctacaaaaa agacgggaat actgcttatg tagtcacgaa taatcacgta
    17401 gtagatggcc agcaaggtct tgaagtcttg atgaaagacg gtacgaaggt aaaagctgaa
    17461 ttagttggaa cagatgctta ttcagattta gcggtgttga agatcaattc agacaaagtc
    17521 gaaacagttg cctcttttgg tgactcaagt gctctgaagg ttggtgagcc tgcgattgcg
    17581 attggttccc cactaggatc tgaatatgca aattctgtca cttcaggaat catttcttcc
    17641 ttgaaccgac aagtgacaag tacgaatgaa tcgaacgaaa cagtcaatat caatgcgatc
    17701 caaacagatg cagcaatcaa ccctggaaat tctggtggtc ctttagttaa tatcgaagga
    17761 caagttatcg gtatcaactc aagtaagatc gcaagtactt ctgaatctac ttctaatgta
    17821 agcgtagaag gaatgggatt tgctattcca agtaacgatg tagtgaatat catcaatcaa
    17881 ctggaaaaag acggaaaagt gacacgtcca gctctaggta tcacaatggt cgacttatct
    17941 gccgtttcta ctcagcaaca agaacaaatc ttgaagattc cttcatctgt aactaacggt
    18001 gtcatcgtga caaatgtcca aacagctaca ccggctgaaa aagctggatt gaaacaatac
    18061 gacgtcatta ctaagatcga tgacaaagaa gtttcttctg gtgtagaact tcaatctgct
    18121 ttgtataaga aaaaagtggg agattctgtc aaaatcactt actatgaagg ctcgaaagaa
    18181 aaaacaacta ctgttgaatt gacgatcgat caatcggcat taaaacaaaa tcaaaatagt
    18241 gaaaattaat aaaaaagagg ttgtgacaaa gacttgtcac aacctcttta ttttggaaat
    18301 gcttctccga ctacggcatc ctcaggcaat actaagattc ctttttttgc tggagcattt
    18361 ggcaaatgca attcacgagc ggaacagatc attccatagc tatctacccc tcgcaaagtt
    18421 cctggccaga tcatcattcc atctggcatc atagctcctg gttttgcaac gacaactttt
    18481 tgtccagctt cgatattggg tgcaccgcaa acgatttgca atactgtacc attgtctact
    18541 tctgtctcag taatcgataa atggtcagaa tcaggatgtg ggacgcatgt ttttacgtac
    18601 ccgacaacga tcttcggttc atgatcagct tcaagctgtt cttcgaatcc tgcttctttg
    18661 atcaatcgat tcaatttcgc taattcctca tctgttaagc ttacttggcc gttcccttcg
    18721 attgttagat gatcggagat atggaaaaag ttccaagcaa caacttgacc ttgttcatct
    18781 ttgatccgag caacattcca tttgcgttca acagtgtttt ctgttccttt atcgtcggct
    18841 ataataacca tcaaggtgtc tccgacgtgt gctttgttat aagcaaaaat catgttcttt
    18901 cctccattat cctttgctga gatttatctt aacaaaaaat tgcattcact gcatgaaatt
    18961 tttttcatgc tttatttccg aagaagcctc ttctggaaca ccatttattc gtaacaaggg
    19021 actgtgacaa gacttttgtc acagtccctt tactgtttta gttaattgat taaaaaggtc
    19081 gtacaagttc cacttgctta attacttggc tgttcttctt cgtcagaaac gatcaagaca
    19141 tcacaaggtg aatgacgtat cacgtagctg gctacacttc ctgtcataaa gcgttcgact
    19201 gcatttagac ctgactgccc aaccatgatt agatcgatct catatttttt tggcaatttg
    19261 actgccatgg attcacgaac agaaccatat gcaataattc cttctatatt tgtgtaatca
    19321 acagatttcc catattcttt acattcttcg atcatttcct tggctgtttc agtttgctga
    19381 tcgactagat tttgatcaat aggtgcatag cccataaagt tataaaattg ctgatcgatt
    19441 acgtttgcta catatacttt cccattatta cggcgagcca cttctaccgc ttttttgaaa
    19501 gcagcaagtg cttgttcact accgtcgata cctactaaaa tattcttata ttcttgattc
    19561 atcttgaccc ctcctaacag atgtcagcct tttgttacac tttctaggaa cgattcgatt
    19621 tcctgttttg ttttacgatc tttattcact agacgaccta attcttttcc gtcttggatc
    19681 acgacaaaac ttgggatacc aaaaatgttc cattcagcgg ctacatctat gtacttgtca
    19741 cggtctactt caataaattg ccaatcagaa aagtcatttt caatttcagg catttgcggt
    19801 ttgataaaac ggcagtctcc gcaccagccg gcagtaaaga aaaatacact ctttccagtt
    19861 tctacgtagc ctgctaattc ttctaaacta tttgggataa tcattctttg tcctctactt
    19921 tctgtttatg tatttttcaa ttaaattata cacctttctt tcgaactaac aaacattgat
    19981 cctcttttta taaaaaaaat ctggcaaaaa gcaatctttt gccagatttt atagtttcac
    20041 tatcgcatta ttttccgtat acgatcgtat ttactgttga tttatccaaa cctttcagtg
    20101 tttgaatcaa catttctcgt gcagcttcaa aatcgcgaat actgaacatc gtttgatgcg
    20161 tatggatata acgtcccact acgccaatca ctgtacttgg gattccttcg ttttgtgtat
    20221 gagcagcacc tgcatctgta ccgccttttg aaacaaagta ttgataaggg atattgtttg
    20281 tttcagcgat atccagcaag tactcgcgca aacgaggaag catgatcaaa cctggatctt
    20341 ggatacgcat caatgtacct tctcctaaat gtccgaatgt gccatttttc gtcactgtat
    20401 catctgctgc agagcagtct actgcaaaga acagatctgg cttgaattta ttgacagaag
    20461 ctttagaacc acgcaagcct acttcttcct ggacattcgc gcctgcaatc aatgtatgac
    20521 ctaatgtttc attttgcaac gcttctaaag cttctaatac cattgtacag ccatagcggt
    20581 tgtcccatga tttactgata atattctttc cgttggctgt tttgatcgtt tcagcgtaag
    20641 gaacgattgt gtctcctgga aggacaccgt aactcattgc ctcttctctt gattcaaacc
    20701 cagcatcgaa caaaatatcg gttacttcta ctgatttttg tccgcttgtt cctcgcaata
    20761 aatgtggggg aacggaagaa gaaatacatg gataattctt accagtagag gttttaagtg
    20821 taaaacgctg agcagaaaca acgtaaggat tccagccgcc tagaggtacg actttaaaaa
    20881 gcccgttttc ttggatttgt gtcagcatga atccgacttc atccatatgt gctgcgacca
    20941 ttacacgggg tgcatccgct tcttcatgat ggcgcagacc aaaaatgccg cccaaaccat
    21001 ctaactgtaa ctcatctacc agcggttcca tatgactttc catataagcg cgaacatctt
    21061 gttcaaaacc gcttgttcct tgtaattctg ttaactcttt gattcgttga aatgtttttt
    21121 cttccatatg ctattcgtct aaatttcaga ctaaactcct ctcagactta tattgatctg
    21181 cggataaaat atgatttttt ctgcagaccg atcgtattta ttatagctta ttttttctgg
    21241 tagcggttat ttatttcaaa gaaaatatga gagtgtggta aaataagcac aaggagtaac
    21301 aagtgagaag aaggaggtat gtccacatgt atgaagaaag cgaactaagc tattttaaag
    21361 gcgggttagc agttggaatc ggtctaggat tattgagcgg ggtagcttca acgatttggt
    21421 atcatcgcaa aaaagtattg tcagcagacc aagtacttga aaatatcaag tcatcttttc
    21481 taaaagaagg tgcaatcgaa ggttcttgga tctcttttga aaaggaacct attaggaaat
    21541 tcgccgttcg gtcgagtgct tatcggggag gtctttctcg aatagaagat ggcgaaactg
    21601 tttattatga atttttagca gatgctcata caggaaccgt attagaaatt tcaagaaaaa
    21661 aaggtgcaac agattaatac gtcaaaaaag tgagagaacg aatacgcacc cactattcaa
    21721 aagagaggta tcatgtcctt cttttgtagt gggtggtttt ttagatagag ctttccaaac
    21781 atatattcca gtattttctt tttaaataaa tgatatttag cgggaaaaac tagcatttcc
    21841 tttgattatg aggtattttt aatttagctt ttgttttcta tagttaagga gagtgaaaaa
    21901 tggaacaaca agaaaaaatt gctgttctga ttccttgtta caatgaggaa gcaacaattt
    21961 caacagtcat tgcagacttt aaacgagaat taccagaagc agatatttat gtctatgata
    22021 ataattcgac cgatcgaaca tacgaattgg ccgttcaagg tggagcaatc gtaaaaaaag
    22081 aacctcgtca aggaaaagga aatgtgatcc gccaaatgtt ctttgatatc gatgccgact
    22141 attatttaat ggtagatggc gatgatactt atcctgcaga agctgtacat ggtttgctcg
    22201 agaaattacg ttctggtgaa gcagatatgg tcattggtga tcgcctatca aatggaacct
    22261 actttgatga gaataagaga cctttccacg attttggaaa taaccttgtt agaaatacaa
    22321 ttactagaat gtataaaaca aaaattcttg atgtcatgac cggctatcga ggattcaatc
    22381 gtatctttgt caaaagtttt cctattatga gttctggttt tcaaatcgaa acggaattga
    22441 cgatccatgc tttagataaa aaattcaagt tcgttgaaat cccaatcgat tatcgtgatc
    22501 gtcctgaagg cagcgaatca aaattgaata cattctctga cggattcaaa gtgatcatga
    22561 tgattattaa aatgtttaaa gattataagc ctttgctttt ctttgggatt tggaccattt
    22621 tcttcttttt atttggattg gtcacgggga ttcctgtgat tcgcgaatat atgctgacaa
    22681 gctttattac acgcatccct tctgcgattt taagtactgg attgatgact ttagcacttc
    22741 tttcattagt aacagggttg attttggata cagtcgtcac gaacgctaag aaagaatatg
    22801 aattgaatct atatcatgtt tatcatgaat accacagtag aaagtaaaaa aaggatcaag
    22861 actgcaaaaa taatttgcaa ttcttgatcc tctttttaat taattatttc gccaaacgat
    22921 agatggcatc tgcatagatt gctgttgctc tgaacaagtc atcaagactc ataaattcgt
    22981 ttgcttgatg cattgtatca gtatatcctg ggaacatcgc tccataagca acgccacgtt
    23041 tcaacaaacg tccgtaagtt ccgccaccga tgatttgttc gtagcctttt tctccagtgt
    23101 ggtcttcata gacttgcaat aatgtagcca ccaatggatc atccattggt acgtaatgtg
    23161 gttccattac gcgagcacct cgagtaactg ttgcgccttc ttgccccact gtttgttcga
    23221 tgtcactttg caatccgtct actgttacac cttttggaaa acggaaattc atattgatga
    23281 agttcgcttc ctctgtacca ttttcttcaa aagcaaatag tccagcattc attgtcaatt
    23341 tgcccatttt ttcatcttca taagcgactc ctagtttttc tccataaaaa tcttcatgga
    23401 cataagcagc tgctacatgg ataaattgtt tagcagatcc taggaattca tagttatcta
    23461 agaacaatgc caagaaagaa cctgcattga taccagattg tgggcttgca ccatgtgcgc
    23521 ctttaccaac taattcgatt tttacatacg tattgtccgc ttcaatcgtt ccgcttactg
    23581 gatttgcttc gatgaattgg taaaatgctt cttccatcgc gatagctgca tctgcacttg
    23641 gtacttgaag tgctgcagtt gctgttcctg gcaccatatt ttcgcgaagt ccggaaacaa
    23701 aagatttcaa gacataatct cctgcattgt cgcctttgaa gcgtaaagct aaagaaacat
    23761 ttcctttttc accattgatg attgggaatt cagcatctgg tgagaaacca aaatctggtt
    23821 cttcttcatg ttcgaaataa taagccatat cgccccagcc gctttcttca tcgctgccga
    23881 ctacgaagcg aacttttttt gatagtttca agtcaagttc tttaatgatc ttcatcgcat
    23941 agtaagctgc catacttgga cctttgtcat cgcttgatcc gcgtgcaaag atctttccat
    24001 ccttgattac tggttcataa ggatctgtgt cccagccatc tcctgcagga acgacatcca
    24061 tgtgtccaaa tatccctagc gtttcgtctc cttcgccaag atcaatatgc cctgcatagt
    24121 tatcgacatt ttttacttta aatccatcac gttcgccata agctaacata tgtttcaatg
    24181 cgtcgcgagg tccaggtcca aatggtgcat ctggtgttac ttttgagtca tcgcgctcgc
    24241 tgttgacacg taaaagattt ttcaagtctt caagcaagtc ttctttacgt gcttctactt
    24301 ctttttgcca atcaattgtc ataaagttaa tcctccactt atttaagtct tcgtgttcat
    24361 gataccacaa attacagaaa aaaatagttc aaaaaatctg acaattcctt ttcttatctg
    24421 tttttctcta aaaaaaatat acgctataat gagtaaaagg aggtttttag atgctgataa
    24481 acgaacaact agtgaaaaga gtcatcacac ctcgtccaaa aatttcacat aaaggaacat
    24541 ttggacgtat cgttttgatt ggcggcaatg aacaatatgg cggagcaatc attatgagtg
    24601 ctttagctgc cgtacacagc ggagcgggtc tcacgactgt agtaactgat gaaaaaaatc
    24661 ggagtgctct tcacagtcat ctgccagaag caatgattgt agtttggacg gatactgacc
    24721 agtttctttc cgtaataaag caagcagatg tactcttgat tggtcccgga ttgggtactt
    24781 cttctaagag tttggaacgt ttcaaacaag tactagaaaa ccaatcaaaa gaacaatggc
    24841 tggtgattga tggctctgct ttaagtttac ttgctgctaa tcatcttttg ctaccctatc
    24901 ccgaacaaac tgtacttaca cctcatcaaa tggagtggca acggttgagt tctctaccga
    24961 tacaagaaca acgggataaa gaaaacaaaa agaagcaaca ggaaatcggt gctgtgctcg
    25021 ttttgaaaag tcatgagaca aaaatttatg gtcaccagca cttctacacc aatcctttag
    25081 gttctgctgc tatggcaacg ggcggcatgg gtgacacttt agctgggatg atcacaggat
    25141 ttttagccca gtttcctcaa aatgaccaaa cgattgcagc agctatttat cttcatagtt
    25201 tcattggaga ccagctggca aaagaacaat atattgtttt gccgacacaa atcagcgaag
    25261 cacttccgaa atggatgaat tatttttgta aaaaatcaat ggaaaacgag gcatgagtca
    25321 tggagaaatt tacaggttat gtgcgagaac ctttttatta tgaaacagat caaatgggga
    25381 ttattcatca cagcaattat atccgctggt ttgaagaggc tcgggtagcc ttattggtac
    25441 atctagggtt tccctataaa aaaatcgaag aatctgggat catcatcccc gttctggaag
    25501 taaattgcag ctataaagaa atgatccatt acggagaaaa agtgagcatc catccaacta
    25561 tccaaaagta tacggggaca cggttggatt ttagctacga aattgtggga actgaagaca
    25621 aaaaattaaa aactactggc agtagtaaac actgtttttt actttctgat tcgcaacgat
    25681 tagtgaagct gagcaaagtc aatccggagt tagatcagtt attccaagcg tacgcttctt
    25741 tggatgatgt aaattcttaa taaagaccgt ctaagcattt ttcttgtatt gtgtcgagta
    25801 gttcgtataa tgaaaagaaa aaagcaggtg atccccatgt atgtcgttaa agtaatgcac
    25861 ggttacatcg ataaaactgg ctgtcgcact cgggaaaaaa acttagacaa tctgctcatt
    25921 ttcaaagaca aaaaagaatc cgaagctttt gctaaaagaa tcggcggtcg tgtcaaaccg
    25981 atccaagagg tacgaccaga ttgattcaat atttttcttt tcgaggctct aataggcatg
    26041 cctatcaggg ctttttttgc agactgtgtt tttatgccca cacgatttgt aagcgattta
    26101 tgttataata gattatatct gcatagaaag gaaggttaaa tggttatcaa taatttgcaa
    26161 cagcaagctt atgaatctat acgtaaacaa atcatttatt ccgagttagc acctggaagt
    26221 aaaatctccg ataaagtgtt agaagaacaa ctagctatcg ggcgtacacc gattcgagaa
    26281 gcactgatcc aactgcgcaa tcaagagctt gtaaaaacca ttccacagtc cggcacttat
    26341 gtttcaaaaa tcgatatccg atctgcttca ttagcccgtt atacgcggga aaagttggaa
    26401 aaaccgatcc tgcaagaatg cagtgcgaaa atgaccaagc aagatcaagc taaactagaa
    26461 aaaatactag aacaaaccga tcaggcagta atcgctcaaa ataaaaaagc tttttttgat
    26521 ttggatactg attttcaccg aacttgttat gaaattgctg gaaaacgaga aatttgggat
    26581 tggctagaaa gttatagtac gcacttgaac cgtttcagat ggttacgttt gacgatttca
    26641 gaattagact ggggtcgagt attagatgaa catcagacaa tgcttcaatc aatgattgat
    26701 cataactttg atgaagtcgg atttctttgc acgatgcatt tacatatgat tatcgaggag
    26761 caagaatacg tgattcataa ttaccccgat tattttgaag atattcagga cagacccatc
    26821 aaataagagg ttttggaatc agcgttttga cctgagtatt agagaaagaa acggcaaaat
    26881 agttcctcat attttgccgt tttttttgag ctaataccga gggtcagctg attgcacacc
    26941 gtttattgag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca gccaagtaaa
    27001 aatagcttct tatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac
    27061 accgttttcc aagaggtcgt gagcctgccg ttcatctccg agtcacaagg aacaatttca
    27121 aaaaacagct tctcatgttt gttgaatatt ttgacttgtg accgaggagt tgacaggaga
    27181 acatcgttta ttcggaaaaa aaggactgtg acacgacttt tgtcacagtc tctttatttg
    27241 taatctaaat catactccgt acatcttttt tctatttttt ttatttcttc taagatccct
    27301 tggctacgaa tttcattata tggccaaagt aagactgcct ctaatacata aggcgaaaaa
    27361 tcgctttgat tcatctcttt tgaaaaactc atcgctgcta tctcgctcaa tgcacgaatg
    27421 tttgattgat gtgtataaaa aggcaaccgc cacagcacaa ttttttcgct ttgcgtataa
    27481 attcccttaa cagaagcttc gatgtggtga aatagttcat gtccaataat caaagcttgc
    27541 gctgtttctt tagagatcga agctagtcct gaacatttta atttttctaa cggctccgtc
    27601 atcaatcgaa tcccttcatc ttctgcataa gttgcaaaag tgattcgttc gtttatttgc
    27661 tctgattcac aggaaacgat tggaacagcc atttgttcac aaatttctcc tacatcggca
    27721 aaagggtact tttttttgat gctacatgcc gtttcgatac cacaatcgat tgattgttgt
    27781 ataagaattt tctgcttgga caaaggaatc tttttccaaa ataaatctcg ccgaaactgg
    27841 tagaatcccc aaatttcttc ggggatctgt tgtaattcgt ttagtaactc ttctaactta
    27901 tgtttgtttg atatttccgg caacgacgat cacttcttcc tccggttcca tagtagatac
    27961 ttccagatcc atcaaaagct gtagttgttc cagatccgtc gctgtaaaat cagagactct
    28021 tgctcctaag cagagataaa attctggtcg ggcaatcaat tcagtttgat aaactgccat
    28081 atcttcccaa agttgttcga cagctgtcat ataattggct gcagttgttt tcatacatga
    28141 agcatgtcca cgttggacct tgataaatcc tttttgatcg atgatccgaa gacttccagc
    28201 attttttgaa cgattctgtt cgccgcatac ataaaagaaa ggtgtatttt ccaataagca
    28261 aacttcattt ttatttaatc gcatatcctc tgcagcaagc tctagtgctt cttctgttgt
    28321 gatctccttg gttagatcgg ttgctttcac ttccgtagaa ccagtagcga tagccgttac
    28381 ttttgaagtc tgtggatcga tttctacgtg gacttcgatc gattctggtg tagcgcctga
    28441 ttcaatcgct ttattcatcg cttcattttt caacgatcgt atatcttctt tacttggcga
    28501 agggatgata cgttctacaa catctcggac cattgcaagt gctacaccaa tagaagaaat
    28561 cacttcggcg ttttcaggaa tcgaatattt cacacccatt ttattactaa agtaagtaat
    28621 caatgaggca gcgccgcccc ctacacctac aagagaaatc tgatctttct ccaagtgata
    28681 cttgtctgcc aattctaaaa tcaccggttc aatcttagca taggattttt ccatgatctg
    28741 ttctgcgatg tcttcaaccg tcgtatgaca ataatcagct aatgcttgga tggcttttcg
    28801 tgcagatggg acatttccat aagaaaaatg ttcttcttgc actaatccaa ggacattggc
    28861 tgcacatgta tttgtaatag tcacttcttc tccgtcttcc atcactacct tgacgtaatc
    28921 agcagggtct ccttctttag gagagaaaaa ttcaacctta ggacctttga ttttttctgt
    28981 ctccgtaaat actgcataat ctagaccggc aatatgtgca gaacgtggac cgacatcgat
    29041 gattcctgac tgattagccc gtaccatcga accgcctgcc acacctagaa cccgaacatc
    29101 taatgaagag atatatgttg ggtggccacc tacaattgaa taatcgattg ctggacgtcc
    29161 atttttaatg accccgatat tggttgttgt tccgcctact tcaaaataaa caccattaga
    29221 cgctctcaag tacatcaaag agcccatgac tgatgctgct ggaccggaaa gcatggttaa
    29281 aacaggtcgt tttttcattt cattgatttc cattactccg ccgtctccgc gcataatcat
    29341 caaagaaaca ttgacccctg cttctcgtac ggaatcttct gtagaggttg ccgtatctag
    29401 catttttggt aaaatagatg cattgattgc tgctgtccga gttcttcttg tcaatccata
    29461 taattttgtg atgtcagaag ccatcgttgt agggatcgat tttaccgatg ctgcttcata
    29521 aacaactcgt tctggttccc cattatctac tccaaatgcc atagaagaaa ccaagacctc
    29581 cgctctttct ctttctaaag aagaaatcgt ttctgaaaca cggtctacat ttaagtgttt
    29641 gacaggtaag aatgcattga caatctctat ttttttctta ttccctaaat cgatatcgtt
    29701 caatctagtt tgtcgttttg ctaaaaaacc ttctaatcca ccttttgcca tcccgattac
    29761 gccgactttt gctacgtctc cttcaatcag tgcattagtc gcttgtgtgg tagaatgtgc
    29821 aacaaacaca acatcttctg gactgatatt attttccagc aaacaatttt gaaaagactg
    29881 aacgacacct gcggctactc cacggacatc atcgtgggtt gttttcactg aagattttcc
    29941 gatgatttcg tgcgtcgcat tatcgattgc gactgctttc gtatgtgtac cgccaacatc
    30001 aattcccatt cgcacaaaac gtttcatttt cttttcctcc attcatctaa tcgtttttat
    30061 cgacgttttg tttactaggc gttcccgtac aataagaacg tgatcaggca taagatcgct
    30121 ccgatgatcc aaccagttgg gatggatttc ttcatatatt ctcgagaagt tacttttgta
    30181 taacctaatc cccaagcaac ccaagattgt gtaatatcta aatgctgagg agcaatcgtt
    30241 gtgattgcaa ataacgggaa caaaaaggct ggcgacatcg ttggattaac agctaatacg
    30301 acagctaaaa tagctgagcc tgaaccgact agattcattg gaccgcggaa aaatcctagt
    30361 ggtgtcaata tagcaaatac gattgccaag actaatggtg attgcggtat ccaatctcca
    30421 aaaatagctg aaaaataagg agaagcatac gcagctgaat tattgaacat cgctaaggtc
    30481 atcaagaaac caaccattgg cgcaacatct actgcgccat ctgtaaataa tttcgcaaac
    30541 tgacgacaga tttctttata acctccgcgc aattttccag taaacagcaa tgcccagata
    30601 cctgctagaa taaaacctaa gatgattgga agatctaaaa gaacgacccc taaaactggt
    30661 aataatacgg ctaaccatga gatcattggt gcgtcgccac cttcagctgg tacgtttgct
    30721 gccattgcat agcgtttctt tttgttcatt gatatattag cgaccgttag taccacaact
    30781 aagcttacaa tcatcccaat cataccaatt tggaaatagt cattataagt atagctttca
    30841 gccgctggat tgaaaccagc ataaatcgtt tgatattgtt tgaaattgac gatattagca
    30901 aagattccag ccataatcga gcccataaat gagaacagtg caaccggtac tgaaatacct
    30961 aatgacatca agattggcaa cacaataact gcaatagaaa tcactggtcc gattcctgtc
    31021 atcgacatga atataacagc ggttacaata catagtaagc tcatcgtgat tcttggcttg
    31081 tcccctccaa gttcaactac ctttcgaatc agggtagctg cgattccgct atctactaga
    31141 acacgcccga aaaaggcacc aaagaagaca tttaccaaga tcgatttggc gtactcagct
    31201 ggccccgtcg tatagatatg cgtcaacaca tcaattactc cttgcccttc catcgccggg
    31261 tttggtgaaa acgtgtttcc gactagcgct aatcccatcc atacagtagc catgatggca
    31321 aagccgacca tcaagttgta gccttttaca cagtaccaga ccatgaaaaa gaatgagata
    31381 actaacaaaa taccaataat aatatcactc atgttattcc tccgtccatt ttgtttttct
    31441 gataagcaaa ctaatatatc agtcaaactc attctaactt atgtagttag cgctttcatt
    31501 aaagggtgac cataaatgtt cagttttcaa ttaaaaaacc ttaattatgc tattttagac
    31561 ttatacgtta ttgtcctgtt tcatttgatg gaggattgta aataaaaaaa gaggaaagaa
    31621 cataactttt cgttatcttc tttcctcttt ataattgttt agcttattta gccaaagctt
    31681 cttttgcttg gtcagccaat gcagtaaatg ctgctgcatc gttaacagcc aagtcagcta
    31741 acattttacg gttgatgtcg atttcagcaa ctttcaaacc gtgcattaat tttgagtagc
    31801 tcaagccatt catacgagct gctgcgttga tacgagcaat ccacaattta cggaagtcgc
    31861 gtttcttttg acgacgatcg cgatatgcgt agttgtatga attcattact tgttcttttg
    31921 ctgttttaaa taaagtgtgt tttgaaccgt agtaaccttt tgctaatttt agcactcttt
    31981 tacgacgttt acgagttact gttccacctt taacacgtgc catgtataat tcctcctaat
    32041 aaaaataaat cttcagattc tgttatttgt acttcttatt tcatttttgc tagttgttgg
    32101 cgaatacgtt tgaagtcgcc actagaaacc atgcttgctt tacgcaattg acggcgttgt
    32161 ttttttgttt tgccgtggaa acggtgactt gtaaacgcgc ggaagcgttt caatccgcct
    32221 ttaccagtac gtttgaaacg ttttgctgat ccgcggtgtg ttttttgttt tggcatgact
    32281 atatttcctc ctcaaaatct ttcgttatcc gactatcggc tgtccaacag ccagttagtc
    32341 attcaaatta cttactgtcg tttttcggtg ccagcgtcaa gaacatgctg cgtccgtcca
    32401 ttttcgcttt ttgttccact gttgcaatat ccgcagtttc ttcagctaag cgattcaaga
    32461 ctttttgacc aatttcttta tgggtaatgg cacggccttt gaaacggata gaagctttca
    32521 ctttatcgcc cttttccaag aacttacgtg cattacgaag ttttgtattg aagtcattca
    32581 agtcgatcgt aggacttaag cgaacttctt ttacattgat cactttttgt tttttacgcg
    32641 cttcgcggtc tttcttttgt tgttcgaaac ggtatttacc gtagtccatg attcgcgcaa
    32701 ctggtggttt cgcaccaggg gcaactaaca caagatccaa gtttgcttgt tctgcaatat
    32761 tcaatgcttc tgcttttgtc ttaactccta attgctcacc atctgaaccg atcaatcgta
    32821 actctcgtgc acgaatgccg tcgttaacca tcatatcctt tgctatggtc attcacctcc
    32881 aaatattatg agagaaagaa aagcttaccg aataaacggc aataaaaaaa cgagttctct
    32941 tacagaaccc gcgcgtactt cacctaatag aaccagtgaa aactatctag cccagtgaca
    33001 ttatcaacta aggcgagaag cgggtgcttc tgcttttatt tctcaaccat catagtatag
    33061 caaaaactaa tctttttgtc aatgtttttc atacagtttg tcttcctttt ctgaaaatga
    33121 ttagattagc agactttcta gcagtgattc ccgcttcttt gctatactaa agaaaaacga
    33181 ggtgaaccca catgaaaaaa atcgaaccta ttccaccttc tcttcctctt cttacaaagg
    33241 gagagtttta tttagaagat gaggagatct tctctggtct tctgatcaac gggggtgacc
    33301 aaagctatct cgatgccaaa aatatggtat taagagagag tcatatccaa aaacttacga
    33361 tgcaaaaaac agttttacgt cattttgaat gcagtaatgt catttttgaa aaatgtgatt
    33421 tttccaatct ggagtggatt ggtggcagtt tccaccaagt cgtgtttcat caatgtaaat
    33481 taacaggtac gaattttgcc gaaagttatc ttcgagactg tactttcact gattgtattg
    33541 cttcgatggc ttcctttagt agtacaaact tgaaagctgt ctccttcgat cattgtcaat
    33601 tagaagacag cgaattttat gaggtcactt ggaaaaacct ttttttgtct gacaatcagt
    33661 tgaccggttc gaactggttt cgaacttctc tgaagggatt agactttacg actaatttct
    33721 tcacagcaat cgctttatct caagattctt tggcgggact aatcgtcaac caagagcaag
    33781 cacttgtgat cgcccaagcc cttggtttgc agatcaaaga ctagatcaac gaatccgccg
    33841 ccacagtgca ataagcatcc aaatagataa gaccaaactg atgatcaagg cgattcccca
    33901 tccgaaaacg gaatgggtaa agggtaactg gagattcatt ccgaagaatc ctgtcacaat
    33961 agttggaacg gtcaagatca gcgaccagac cgtcaaaaat ttcatcgtgt cattcaagtt
    34021 gttattcagc aaattattat aagtgccaga gagctggtct aaaatctgcg aagctaagtt
    34081 cgtcatttca actgcttgtc gtgcttcgat cagcgcatcg tccaactctt ctttttcctt
    34141 ttctgacaac ttgcgataaa tagccaatgc cttgatttgt tctaaaagta ctacattttg
    34201 ttttgttcct gtaactaagt aaaccaaacc gatctctaaa tctgaaagtt gcagcagatt
    34261 cttgttcgtt gttttttctc ttaatttcag attaagccgc tgacgttcac tattcacttc
    34321 ctcgattaaa ggaaaaaagg aatcagaaat caaaaacaat gtacgaaaca ataaactgta
    34381 tacactcatg tctggcattc gttccaaaag cgatcgaatt agtcgaatca cgtactcggt
    34441 attcggcgtg gtaaatgtaa acaaaccgtc ttcttttaaa ataaaagcga taggactagt
    34501 ttcataatga ttatcgactt tttgctggtg cggaacattt gatacaagca gtaacgcttc
    34561 atcaaaagaa tcgtactcta cgcgcgcccg ttcattttta tctagagaat aagtcagcat
    34621 ctcgtcacta atgccatatt cttcttttaa gtttttaaca gctagcacat cgttttgctg
    34681 tacattgatc caaaaattac ggtcttgatt aaaatagtgt ttttcccgca tactctatcc
    34741 tcctgacatt tgtttccagt ataaagaaaa aagtcgctca aataaaggtt taccctttat
    34801 ttgagcaact gtaaatcaaa tggctacttt ggtttggcga atctctgcta atttttcttc
    34861 gatcattttc aagacttctt cttttgcttg aggatcttcc acaaaattat agcggtctcc
    34921 atcgatttgg atttttggac tttctttgta ttcttcatac cattgatcgt aacgtctgtt
    34981 caattctttg taataatcgt atagacttgg atcaaaggac aattgttcat acgaacgacc
    35041 acgtttttcg atacgctcca acatcgtatc aaaagataca cgaatatgaa ctaataaatc
    35101 cggatgtttt ttatgggcag catagggaag ttcttccatc atgttttcca gcagttcatc
    35161 atacacacga acttctgttt ctgttgctct tcctagatct gcatttagat ggaaaagcag
    35221 tgaatcttca taaatcgaac ggtccaatac attattgtca tctttcattg cttgtttgat
    35281 gcttgcaaaa cgtttattca aaaaataaat ttgcagcaaa aaagcatact gttcagggtt
    35341 tgcataaaat aagggcaaga cttcattatc atcaatcgat tcataaaatg cctgactatc
    35401 aaagtgttca gccatcattt ctgttaaact tgattttccg gcaccaattg tacctgctaa
    35461 aacgatcacg ctcatttacc tccagctcac ttcatcacta aatattgtgt ttaactccaa
    35521 cctttttaaa tataccccca atatctagta gtgtcaattg tatttttttc tagtcaaaga
    35581 agaaaaaaag tcaaaaaatt cttaagaaaa aagtgttttt tctttagaaa acgtattctt
    35641 agttttactt ctgagatggt tttagtaaaa taaaggagga atgttttttg agacccttag
    35701 tggacaaaaa aattcgtgaa gcagcgcaac tcgttttggg cgctttattt tttgcttaaa
    35761 aatcactttt acgtgagaag gagttagatt atggcaaact atggccaagg agaatcaagc
    35821 ggaaagatca tattgatggg cgagcacgcg gttgtttatg gagaaccagc gattgccttt
    35881 cctttctatg caacaaaagt caccgcattc cttgaagagc tggatgcaat ggacgatcaa
    35941 ctggtttctt cctactattc aggaaattta gccgaagctc ctcatgcatt aaaaaatatc
    36001 aaaaaattat tcattcactt aaaaaaacag catgacatcc aaaaaaactt gcaactgacc
    36061 attgaaagca cgattcctgc tgaacgtgga atgggatcaa gcgctgcagt cgccacagca
    36121 gtcactcgtg ctttttatga ttacttagca tttcctttgt ctcgtgaaat actattagaa
    36181 aatgtccagc tttcggaaaa aatcgcccat ggtaatccta gtggaatcga tgcagccgct
    36241 actagcagct tgcagccgat ttattttaca aaagggcatc ctttcgacta cttttctttg
    36301 aacatcgatg cttttttgat tgtcgctgat acaggaatca aaggacaaac aagagaagcc
    36361 gtcaaagatg tcgctcacct ctttgaaacc cagcctcatg aaactggaca aatgatccaa
    36421 aaattaggat acttgacgaa gcaagcaaaa caagcaatca ttgaaaattc accagaaacg
    36481 ttggcacaga caatggatga atcacaatca cttctggaaa agctgacaat cagcaatgat
    36541 tttcttaatc tattgatcca aacagcaaaa gataccgggg cattgggtgc caaattaact
    36601 ggcggtggac gcggtggctg tatgattgca ttagcacaaa caaaaacaaa agcccaagaa
    36661 atcagtcaag cacttgaaga tgcgggtgct gctgaaactt ggatacaagg attaggagta
    36721 catacctatg tttaaaggca aagcacgcgc atataccaac attgctctaa tcaaatattg
    36781 gggtaagaaa aatgaagaac tcatccttcc aatgaacaac agcctttcgt taacattgga
    36841 tgcattttat acagagacag aagtcatctt ttctgatagc tatatggtag atgaatttta
    36901 tctagatggc accttgcaag acgaaaaagc aacaaaaaaa gtcagtcagt ttcttgacct
    36961 ttttcgtaaa gaagccggcc tctcactaaa agcttcagta atcagtcaga atttcgttcc
    37021 taccgcagct ggattagcct cctctgccag cgggctagct gctttagcag gcgcttgcaa
    37081 tactgctctt aagcttggat tagatgatct ctctctttca agatttgctc gacgcgggtc
    37141 tggttcagct tgccgaagta ttttcggtgg tttcgtcgaa tgggaaaaag gccatgacga
    37201 cttaagttct tacgctaagc cagtcccttc cgattctttc gaagacgatt tagcaatggt
    37261 tttcgttttg atcaacgacc agaaaaaaga agtgtccagc agaaatggga tgcgtcggac
    37321 agtcgaaaca tccagttttt atcaaggctg gttagattcc gttgaagggg atctatatca
    37381 attgaaacaa gcaatcaaaa caaaagattt ccaacttctc ggagaaacga tggaaagaaa
    37441 cggactaaaa atgcatggaa cgactttagc tgcccagcct ccattcactt actggtctcc
    37501 tgacagctta aaagcgatgg atgcggtgcg acaattgaga aagcaaggca ttccatgcta
    37561 ctttacgatg gatgctggcc ctaatgtcaa agttttagtg gaaaacagcc atttatctga
    37621 ggtacaagaa acatttacca aattatttag caaagaacaa gtaattactg cacatgctgg
    37681 tccaggaatt gcgattattg aataaaggga gatttttatg attgaagtat ctgcaccagg
    37741 caaactctat attgccggag aatatgcagt tgttgaaaca ggccatccag cagttatcgc
    37801 tgcagtcgat caattcgtga cagtcactgt agaatctgca cgaaaagtcg gaagtatcca
    37861 atctgcacaa tatagtggga tgcctgtacg ttggacaaga cgcaacggag aattagtttt
    37921 agatattcgg gaaaatcctt ttcattatat ccttgctgct attcgcttga ctgaaaagta
    37981 tgcacaagaa aaaaacattc ttttatcctt ttatgatctg aaagtaacaa gtgagttaga
    38041 cagttcaaat ggccggaaat atggattggg ttctagcggt gccgtgaccg ttgcaacggt
    38101 caaagccttg aatgtttttt acgcgttgaa tttatctcag ttggagattt tcaagattgc
    38161 agcactagcc aatttagcag ttcaagataa tggttcctgc ggcgacatcg ctgctagctg
    38221 ttatggtggc tggatcgctt tctcaacctt cgaccatcct tggctccaag aacaagaaac
    38281 tcagcattct atcagtgagt tacttgccct ggattggcca ggtctatcca ttgagccatt
    38341 gattgctcct gaagatttac gtttattgat tggttggacg ggtagccctg cctctacttc
    38401 tgatttggtc gatcaagttc accgttcgag agaagataaa atggtggctt atcagctttt
    38461 cttaaaaaat agtacagaat gtgtcaacga aatgatcaaa gggtttaaag aaaataatgt
    38521 aacgttgatc caacagatga ttcgaaaaaa ccgacaatta ctgcatgatt tatctgcaat
    38581 cactggggtc gtcatcgaaa cgcctgcttt gaacaaattg tgtaatttag ctgaacagta
    38641 tgaaggagcc gcaaaatctt ctggtgcagg tgggggcgat tgcggaatcg taattgttga
    38701 ccagaaatct ggcattcttc ctttaatgag tgcatgggaa aaagcagaaa tcactccact
    38761 gccgttacat gtctatagcg atcaaagaaa ggaaaaccga tgaatcgaaa agacgaacat
    38821 gtttcactag ctaaagcctt tcatgataaa caaaaaaacg aatttgactt cgtacggatc
    38881 atccataacc ctttgccgca aatagcagtt tctgatgtcg atctaagtac acaggcagtt
    38941 gggtttacac taagcagccc attttatatc aatgcgatga ctggcggaag cgaaaaaaca
    39001 aaaaaaatca accaagatct tgcaatcgtc gcaagagaag cagatttgat gattgccact
    39061 ggttcagtca gcgctgcctt gaaagtccct tctttagccg atacttatac gattatgcga
    39121 caagaatatc ctcacgggaa aatcattgcc aacatcggtg cagggacttc tgtcgaaaga
    39181 gccaaagaag ctatccgatt gtttcacgca gatgccttac agattcattt gaatgcacct
    39241 caagagttgg ttatgcctga gggagaccgt gattttacca attggaaagt acttattcaa
    39301 gaaactcaaa cagccattga cgtccccctc atcgtaaaag aagtcggttt tggcatgaca
    39361 agagaaacgc tcaatgatct agctgcttta ggagttcata cagttgatat cagcggtcga
    39421 agcggaacaa gcttcaccca gatcgaaaac gcccgtcgct ccaagcggga actgagttat
    39481 ttagctgact gggggcaatc gacagtctct tcattgcttg aagcaaatga agcagacact
    39541 tctatggaaa tcctagcctc cggaggtatc cgcaatgcct atgacatatt taaagccctt
    39601 tgtctcggcg caaatgccgt gggtacttca ggaaccgtac tgacgcatct gatgaatcac
    39661 ggcgtagaag aaactattat actgatgaag caatggcaag aagagcttcg tctgctttat
    39721 actatggtcg gcgcaacgaa taccgcggcc ctacaccaac aatctctgat cttttccgga
    39781 ccagtgaagg attggtgcga agcaagaggg atcgacttag tgaagtatgg aagacgcaca
    39841 gaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa cacgtacaaa
    39901 cggcggaagt aaaagcaact ccttcggaaa taagctgaaa ttcacaaaaa tttgaaaagc
    39961 aattttcgtg aattccttct tatttcttgg agttaaatgc ttctgtccca gcctcttttt
    40021 tcgaataaat gatgtttaat cagctgtcct tcggtattcg ttcaatccgc tgatttcaca
    40081 acctctccac taaacagtgt actaaagctc ttttaaggtt gttttccgat ataggcaagt
    40141 atgcctccgt ccacatacaa gacatggccg ttaacgaaat cagatgcgct agaagccaag
    40201 aagacagatg gtccagccaa atcgattggc tcgccccagc gtgctgctgg cgtacgtcca
    40261 acgataaatt gatcgaatgg atgacgttca ccgttttctt gtgtttcgcg taatggagca
    40321 gtttgcggtg tagcaatata acctggtcca attccattac attggatatt gtattgaccg
    40381 tattcagaag cgatgttttt cgtcagcatt tttaatccgc cttttgccgc agcataagca
    40441 ctgaccgttt cgcgtcctaa ttcactcatc atggagcaga tattgatgat tttaccgccg
    40501 cctttttcga tcatatccgg aataactgct tttgaaacga tgaatggggc atttaaatcc
    40561 acgtcgatta cttggcggaa gtctgccgca tccatttcga tcatcggtgt ccgtttgatg
    40621 atccctgcat tgttgactag aatatcaact gaacctacat cttctttgat ctgttgaacc
    40681 atcgcttgaa ccgcttcttc atcggtcaca tcgcagacat aaccttttgc atcgatgcct
    40741 gcttctttat aatgcgctat accttcatca actgattctt gattcaagtt attaaatacg
    40801 atttttgctc ctgctgtagc taaagagcgt gcaatctcaa atccaatacc atagacagca
    40861 ccggtgacta atgctacttt tccatctaaa cgaaacatat ccatactaaa ttccatcatt
    40921 ttctctccct tatgcttatt ttaattgatc catttgcacc atatccatgt ctgtataggt
    40981 gatattttct ccgcacattg cccagatgaa tgagtaattg cttgtaccta cacctgaatg
    41041 gatcgaccag cttggcgaaa taatggcttg ttcattgtct acaactaaat gttttgtttc
    41101 atctggtttg cccatcatgt ggaacacacg tgtatcttca ttggcataat caaagtagac
    41161 ataagcttcc atacgacgtt catgtgtatg acaaggcatc gtattccaag cgcttcctgg
    41221 ttctaaaatc gtataaccca tttgtaattg acagctttcg catacatttg gatggatgta
    41281 ttggtagatt ttacgttcat tcagtgtcag gccttcacca gtctccattg gttttacttg
    41341 gtcgatactg atttttacat ttggatattt atgatgagct ggaacagaac taatatagaa
    41401 tttagctggg ttttcagcat cttgagaaga aaatacaaca tgtcgtgttt cttttccgat
    41461 atagtatccg tcttgttttt tcatttcttc tttttgccca tcgatctcga tgaatcctgg
    41521 tccaccgata ttgatcacac ctagttcacg acgctctaag aagtaatcaa cacctagttc
    41581 tttgtctaat gtgatttcta attctttttc agtaggtgtc actccaccaa agatcattcg
    41641 atcgttgtgc gtataagtca aactgatttt ccctggttca aatacctttt ctactaaaaa
    41701 ttcttttctc aattgttccg ttgaataatg acggatatct tctggactgt gtgtataacg
    41761 tgtttccatt tcttgcattt tcttctactc cttctatcga atgatttttc cagagccgca
    41821 tgctaaaaat tgtgcgactt cttcttgact aaattggttg cagtccccat ggatcgtatg
    41881 cttcaaggca gaagctgccg tagcaaaatc aatgatttct tgcggagaca tattggataa
    41941 aatcccatgg agcacgccac ctgcaaaagc atctccgcca cctactcgat ccacgattgg
    42001 ggtgattcta tggacttggg attccacata ttcatggttc atccacaatg tccccttcaa
    42061 ttcattttca cttgctgaat gaacggtacg cttggtcgaa taaaacacct gaatattcgg
    42121 gaacttttcc tgcatttttt gataataata aattagaggt tgttcttctg cttctggcgc
    42181 tttctcgata cctaacaaat aaagagcatc catttttcca gctgagcaga tatctactaa
    42241 aggtaaaagc tgatggatta cttcgctggc ttcttgctgt gtccataatt ttccacgata
    42301 gttgatatcg aagctgatca ggcagccagc tttttttgca gcttctatca cctgtttcgt
    42361 cattttttgc cattttttag acaaagctgg ggtgattcct gaaacatgaa atatatctat
    42421 tccttggaat aactcctcta gcgaccattc caaatgatcc atttcagcaa aacttgatcc
    42481 ggcacgatca tagatcactg aggcggcgcg ttcgccacta ccagtttcca tataataagt
    42541 acctagtctt gatccaccag ataatacctg tttcgtatct actccataac attgcaagtg
    42601 ctttttgact gcttttccca atgcgttatc aggtactttt gtagcaaaaa tcgcttcatg
    42661 tccgtaatta gctaaagaaa ttgctacgtt tgcttcccct ccgccataat gggcagcgaa
    42721 tcgttcactt gaatcgatac gggttccctg ctctgtggac aatcgcaaca tgatttctcc
    42781 gagtgtcact acttttccca taagcagcta cctcttgatt tctttcattt ttgctgcata
    42841 ctgttgcgcg acttccgtta ctttgtcaaa atctcctgta gctgcgggtg ctaaaaggtt
    42901 cccacctact ccgactgtta cgacaccagc atcgaaccac tctttcatat tgtccagact
    42961 cacaccgcca gttggcatga tattgacttg aggcatcggc gccttgaagg cagaaatgat
    43021 acttggtcca taagcgcttc caggaaatag tttgacgata tctaccccgc ttttcagcgc
    43081 tgttttgatc tcggtaatag tcatacatcc tggtaagtaa ggaatttgat acagattgca
    43141 catctctgcc gtttcttgat caaaggaagg gctaacaata tactctgcgc cagctaagat
    43201 agccaagcga gctgtgacag cgtctaatac tgtgcctgcg ccaatcacta tttctggttg
    43261 gtctttgtag aatgacaaaa gttctttgat tacttgatct gcttgaggga ctgtaaaagt
    43321 caactcaata ccagttagac cacctttgat gattgcgtgg caagcattca aagcttcttc
    43381 tttgcttttt ccgcgtacca ccgcaatcac tccggcgttt ttcaatcgag ataaaatatc
    43441 aatttttttc atcttctcca cctatttcct atttagaaat tttattttat aacttggaaa
    43501 tctataccca tattatacgg cagaaaagaa aaattgcaaa cgcttttttc attatttttg
    43561 aattctttgc tcgatttcgc tttttgtttg cttgagggca ctgattattt gttcttttgt
    43621 ctcatctgtt aaacgatatt tcggcatact tacactaaat gcaccaatga tttgttcgtt
    43681 tttcacgatt gaggctccta tacaaaaaat atcacgttcg atttcttcat cgtcaaatgc
    43741 gatgtgggtt tcttttaccg ttttgatttc ctttttcaac cgtaatgcat tggtgatcgt
    43801 atattctgtg taagggatca acggatgctt ttcaagataa tcagtgtact gctcttcact
    43861 gaattctgct aatacggctt tgcccatcgc agaattgtat aatggtcggg tgattccgat
    43921 ttttgaagac atgcggatcg tttgatgttt tggatctagt ttgttcacgt acaagatctc
    43981 attgttgtcc aataccccta agtggatagt ctcatcgatc ttttgctgca gctgttctaa
    44041 aaacggtaaa gtcaactcta ccaaatcgat ttgttcaatg tttttattcg cgtagcgaat
    44101 aagctttgca cctaaacgat agtttttctc tgaattttta tttacgtagc cgatcatcaa
    44161 caaagtatct agtattttca agactgtaga agaggtcatt cccacaccct ttgcgatcgt
    44221 ctgcaaagag acatcttggt tttccgctaa atagtctagt attttagagg ctttgatcaa
    44281 tacagttcca taaggctttt tattttccat ataatattct cctatttctt tataagaaaa
    44341 attatagcgg aaataccatc aatttaaaag agatatccag atataaacta ccatttctct
    44401 ttacaggcaa tttaatgttg aagatccgct tcgtttttac atcaaacttt gatataaagc
    44461 aatgatttct tctactgtcg tttctcttgg atttcctggt gtgcatacat ctcgcaaagc
    44521 atcttctgca attgctggaa tatctgcttc ttttacacct aattcagaaa tggtttctgg
    44581 gattccaacc gctttgctca accgatcgat ctcaccacaa gccgcttctc ttacatcttc
    44641 taaagacatt tcagctgctc ctttaatccc tagaacaaga gcgatctcac ggtacttttc
    44701 tccagtatat tctttgttat atttcatgac agttggcaaa agtgctgcac acgctacgcc
    44761 atgaggaatg ttgtaccatg cacttaacgg gtgagccatc ccgtgtacca gtcctaaacc
    44821 gacatttgag aagcccattc ccgcaatata ctgtcctaat gccatttttt ctcgcccatt
    44881 ttgatcgcca tctacagaag cttccaaaga atggacgatg atttcgattg cttttaaatg
    44941 cagcatatca gtcatttccc aagcgccttt tgtgataaac ccttcaatag catgcgtcat
    45001 agcatccata cctgttgatg cagccaattt tttcggcatt cccatcatca tatcgctgtc
    45061 gatgaacgcg acgattggga tatcatgagg atcaacacaa acaaattttc ggtgatttac
    45121 ttcatctgtg atgacgtagt tgatcgttac ttctgctgct gttccagaag tcgttggtat
    45181 agctaatata ggtaaacatg gatttttcgt atcagcaaca ccttctaaac taatgacgtc
    45241 tgaaaactct ggattcgtca aaataatccc aatggctttt gccgtatcga ttggcgaacc
    45301 tccaccgatt gcgatcaaac agtctgcctc tgcctcttta acaaaagcca ctccatcttt
    45361 tacattttgg atcgttggat taggtacgat cccgtcgtat aaagcataag cgattgctgc
    45421 tttatctaga agttctgtta cttttgttgc tataccgtgc tcgatcaatc ctttgtcagt
    45481 aatgacagct gctttgccga atcctcgctg ctgaaattca gggacgatcg actcaatcgc
    45541 tccttttcca aaatacgatg tttcatttaa aatcattcga ttactcattt tctattcctc
    45601 ctaatttgta ttatttttca caataaaaac gaaagaaccc caagcaaccg gttgcccagt
    45661 tgcttgttgc tacagttcaa agactggcat caattcccaa ctgactggag aggcatctgg
    45721 atttgtctcc atgatatctt ccatgtactt ccaccatttt tgattgatag atgtatttgc
    45781 cattttttcc cataactttt catcttcgat cactaagtaa ccaaataaca aacttgtact
    45841 cggatcaaga tagatcgaat agtcgatgac cccgtgttca cgaagtgctg tacgcatttc
    45901 tggccaaagt tcatcatgtc tttttttgta ttcttcttgt ttttctgggt aaactttcat
    45961 gcaaaaagct tttttcacca tgtcagacac ctgctttcgt atgaacttcc tccaaataac
    46021 ctgcttttgg agtgacacca aacgcatctg ctaaatgcca caggttttcg tccgtgattg
    46081 tttgcaagat tggtccttgc gctttgacgt acgtatagac ttctgctgcc ttttctgctg
    46141 tctcgatcaa accaaagact tcatccatgt cttttcctga cccgtagatt ccatgttgtg
    46201 gccataaaac caatcgagtt tctttcattt tttcagctgt tgcttcccca atctcatttg
    46261 ttcccggaac catccacgga atgatggaaa ttccttctgg aaaaacaact aagcattccg
    46321 tacacatttg ccatagcgta cgactaaagg aacgttcgtc taattcatgc gtaaaactca
    46381 tcgctaatag atgactagca tggcaatgca taacgatccg attatctggg tctacttcta
    46441 aacgggcgat atgactcatg aaatgacttg gcaactcgct tgtaggaagg cattcgtttt
    46501 ccagccccca caaaagttcc agtgttttgc catcttcagc aactcgaacg attccaagat
    46561 tctcggcagg ttcgctcgct acatttttaa aatatcttcc tgaaccagtt acaataaaat
    46621 agcatcctgc taaacggctg gcatcaaaaa tcatcgagat gcggcggatc actttttcag
    46681 gatccaagaa cggtgtgatc tcttcttctt ttagtaaata agaaatattc ccgccatttc
    46741 tttcatccca tcccaagcga taaagattcg acgtcgtttc gatcatttct ttcacataag
    46801 gcgcttgtaa aatatctatt tttttcaata tagccacact cctattttct taattccaac
    46861 acgtcttttt gatattcctg gacttcttcc agccaatcca aaccaactgg aacttgattt
    46921 ttgtaacaga aataattcca tacatcggca tacgggaaat ctttcaattc ttccgtataa
    46981 gccattcttg aagtaaagtc aaaagccagc tccatttctt tcaatcgctc aactggttcc
    47041 agcatagctt tcagcagtgc tttttgtgta tttctcgtac cgatgaccca agcagcgata
    47101 cggttgatcg ttgcatcaaa gaaatccaga ccgatatttg ttttttccaa caattcgtta
    47161 cggactaatt ctttagcgat ttcctgcaat tcatcatcca tcaaaaccac atggtctgaa
    47221 tcccaacgca ctggacggga aacatgcaac atgattcctt tgctgaataa agctaatgaa
    47281 gataatttat tggaaatcac ttctgttgga tgaaaatgtc cagcatctag acaaacaagt
    47341 ttgtcacgag tcaacccgta acccatataa aattcatggg aaccgactgt ataagcttca
    47401 gcgcctaaac caaatagttt gctttctact gcatcttgta cataccgttc atctaactct
    47461 tctgtaaaaa tttcatcgag tgattccatt aaacgttccc gtggggtaaa acgatcaatc
    47521 ggattatctt tcatcccatc aggtacccaa aagttattga tacttgtttg cccagtttct
    47581 ttcccaaagt actccgcgat tttgcggctt cttttcccgt gttcgatcca gtattcacga
    47641 acttctttat ctggatgagc caatgtaaag ccgtctttca tcattggatg cgaaaagaaa
    47701 gtcggattga agtcaagacc gatttcttct ttttttgccc actctaccca tttttcaaaa
    47761 tgtttcggct caatttcatt tagatcgaca gattcttctg tatccaaata gatagcatga
    47821 aggttcaatt tgtgtttgcc cggtatcaac gaataagcct tctccagatc ttgtctaagc
    47881 tcttcaggtg ttcgtgcggc tcctggaaaa ttccctgtcg ccatgattcc accggtcaat
    47941 tcgccatccg gtgtcaagaa acctttgaca tcatctcctt gccagcaatg catagaaatt
    48001 ttgatgtcct gtatcttttc tagtacagca tctgtatcta caccgatagt tgcatatttt
    48061 tcttttgctt gttcgtaatt tctttcaatc gtgttcatac gattactcct ttgattttat
    48121 tcacgtacca ttccatcgtg ttttgcttat tttcatctgg gtaaaaagtt tcacatggaa
    48181 acgactgttt gattacattt cttgcttgtt ctatattcga aaactgacct gtactgagca
    48241 tttgcatggc aacattcccg attgctgtgg cttcactagg tcctgcttct acaatgattc
    48301 cggcataatc tgctgtcaat tgattcagta gacgattatt cgatccgccc cctacgatgt
    48361 gcagcttttc caatttcgtt ccagttaagt gttctacttt gatcagctct tgtgcgtaac
    48421 aaagagcaag attatcatac acacaacgag cgatagctcc cgatgagaca gggactggct
    48481 gctctgtttc aaaacagtat tgctgaatct cttctatcat atttggggga ttcaaaaatc
    48541 gagggtcatt gatttccatc acttgctgca gcggtttttc ttttgcagca agctctgcca
    48601 attccgcata actgtactcg tactcttgca ttcgggcaac ttcctggatc aaccacatcc
    48661 ccatgatgtt tttcaaaaaa cgcaccgtgt tatgggcacc ccattcattc gtataatttt
    48721 cagaaaaagc attgcgagaa acattggcga tctttgtttc aatccctaaa agcgaccatg
    48781 tcccactgct gatataaccc caacgttctc cttgacctgg cgttcctaat acagctgacg
    48841 ccgtatcatg acttgctact gtgataaagg tcacatctgg caaatcatat tttgaaaatt
    48901 gttctttttg cagatctcct agtatcgttc ccggctctgt caatggtgga aataattcct
    48961 tcgagacacc gattttttca agaagttcta tttcccattc ttttgtccct gcattcagca
    49021 gctgcatagt agatgcatta gttttctccg tcaccatgtt accagtaaag acatacccta
    49081 aataatcagg tatcagtaac aatttatgtg cttttgccaa attttctttt tcttcagcaa
    49141 acaattggaa caaggtattg aaaggctgga tctgaatccc tgtctgctca tacaacttat
    49201 ctagagaata gatttcagaa aactgttcta ctgctccatt ggtacgcttg tcccgataag
    49261 caatcggatc atttatccga tccccttgtt catcaatcag acagtaatcg actgcccatg
    49321 tatcgatccc cgcaaaaagg cgatcaatcc ctgtctgctt taatttttca aatccaatca
    49381 aaatctcttt gataagataa tcgatcttcc agcgatcgtg accgtctttg ttccgaaagc
    49441 cgtttttgaa ccggtgtact tcttccaaga tcaaagaacc ttttgcatcc ctcgtacttt
    49501 tcattagtcg tccgcttgat gcaccgatat cgatggcaac aaatgttgga ttcatttctc
    49561 cacctctttt actttattgt tgattagcac tttattaata aagcgttatc atttattgac
    49621 aatgctatca taatacagaa tcttatattt tgcttcacac tattttggta tataattgca
    49681 ttatcttgac aagttgggag tgtttacatg gattactggg aaaaagccaa ccactcatgg
    49741 tcggaagact caaaaagagt gatcttgacg cctacacaaa gaagcaggaa tttatttttt
    49801 tatatccaag aaatcggtta cttcaaagca ccaatccctt actatactga acgagcacat
    49861 ttaccttctt acttgataaa gtttactttg tctggcaaag gttcgcttac atataatgat
    49921 aatcagcaac agctaaaagc tggagactta ttttttattg actgccaaga atatcagcgt
    49981 tatcaaacga tcagcgaaga accatgggaa atggattgga tccatttgaa tggtcagaac
    50041 gtccaatcat tttatcagga gttcacaaaa gacggccata acaccttcca ttcaacagga
    50101 gatccatcag aaaacaagat ccaccatttg atcacagaat tgatccacat gcaacaaggc
    50161 acgaatgccc gtaccgattt ccagtcctct gtagctatcc acgagttgct gaatgaactg
    50221 attttgcaaa agtatcagct agatttcgaa gaagaggata ttcccgccta cgtttacaaa
    50281 ctgaagcaat acttagacga acatttcaaa gaaacgatta cacttgatca attagaacaa
    50341 caattcttat tgaataaata tcagctgagc aaagattttt ccaaatttat cggttctcca
    50401 ccaatcgatt atttgatcag caaaaaactt tcttacgcta aagaccttct tcggtataca
    50461 gaatttacgg ttcgaatgat ttccgaagaa atagggattg agaattttgc ttactttagc
    50521 cgcctgttca aagcaaaaac aggactatcg ccgagagact ttcgtaaatt cagctgattc
    50581 atataatatg tacgtcattt tttcaaaata ttttgaaata tatagataaa aataaaataa
    50641 ccgcagtttc tgcttctatg taaacaacag cagctactgc ggttattttt tagtgatcat
    50701 tctcattgtt catcttcaaa atgttcataa tgaaccaact gtttctgtgt atcacgtttc
    50761 aaaaattctt taggcgttat gccgaactct ctcttgaatg ctttcagaaa atgcggataa
    50821 gaagaaaaac cacttttact atatacagca ctagagctgc ggtattctcg caacaagtgc
    50881 ttggcataga tcagcttttt cttcattaca tactgatgaa acgtaaatcc agtgtacttt
    50941 ttaaattctc gtgtgatatg gtatttgctg acaaaaaact gcttttccat ttcctctaaa
    51001 gaaagcggat gagataaatt ttttgagata tattgtaaca tttgttccac acgctcatta
    51061 ggaactacag cattctgttc ttgcgcactc tcttcttttt gtacaacttg attgagaaaa
    51121 atcaagtatt ctaccagagc ttgttcatat tttatgtctt caccgtaaat ttttttatca
    51181 ggaggatggt cgataaatga taatttttgc ttcaatactt cctgttctat ttgcaataca
    51241 cgactcctct tctctcccat tggttgaaaa caagccgata gattagacca tggcgtggaa
    51301 cgactttcta gaaaagaagg ggtgacaaag atatagacac gctcgaatac atctgaagat
    51361 tggcggacga tacgatgcaa atcattggaa tggatgagca gcacacttcc tgctttgatc
    51421 gtaaattgtc gtccgttcaa ataaaaagtc gcttctccct ttaatgtagc atggatctca
    51481 tagaaatcat gatgatgata gatcgtttta ttttggctga cgacatcttt gacatgatag
    51541 atttcatact gatcagaaac catcgtattg acttgttcac cgctgatttt cttttggagc
    51601 atgtgccgca cctccttttg ttcacttatg cacaatttta ttataccacg ttttttgtaa
    51661 tcaaaaagag ctgcttttat atactcttat aaaaaagaag agacgtggca tcttctagcc
    51721 ccgccccctc cattctaaat attagttaac caacgtcttc tttacattat tcaagaagac
    51781 ttcttccata acgaatccca tcatcttttg tttcttcaag aaccacatac aggtacggac
    51841 gattcttcat gatgatttct ttgatttttg tgtactcgat cacaccgtct cccaaattta
    51901 ccggcacgat tttttcctgt tcgacacgaa aatctttcaa atgaaccgca catatctttt
    51961 caccaaaacg ttcaaaagct tcttctacca attggacttg ttcttgataa tcactacttg
    52021 tgatcaaatt ggtcggatct aaaatgattc ctagatagtc cgaatctact gcttggatca
    52081 gctgctctac ccttgctaaa gaatacaccg gatgattcaa acctggttct atccccacca
    52141 tcatttggta tttttctcca gcttttacca agcgacggat aacggacaca gcttcagcaa
    52201 atgcttcatc tgtgaaattt tcttccgtat attggatttc cggcaaaaca catccagttt
    52261 ccgaagcaac catcgacgca ccaaaggaag aagcatagcg aagatactgc tcgaactttg
    52321 tcagcaactc ttgtcgtatc gttaaatctg gatgaatcat attgatatag caacttaaaa
    52381 ttcctacggt cacttcttgc ttttctaaaa ctcgtttgac ataatttccc atcccagaat
    52441 tgatctcatt tgcgccagaa ggtatttcag gaaacgaaag acctaaggcg agctgcagtg
    52501 tatgaatccc ttgttctttt gttttttctg caagatcttc tatcgtttgg acttcggaaa
    52561 gatcatgtcc tctaattgta atgttcaaca taactattct cctaactcag catattgggt
    52621 agccacatga cgatttgcgg gaagtaagta atcaaaaaca agacgactaa gataacccca
    52681 taaaaaggaa gaagcggttt cagcattgcc tctgctttta cgcctcctac acttgcaccg
    52741 acatatagtc ctgtacctac tggcggcgtg atggagccta cacacagatt catgattgaa
    52801 aacaacccgt aatgtacagg atcaacccct acacttgtga cgattggtaa aaatattggt
    52861 gtaaaaatca agatagcagg cgccacatcc ataaacatac ccaccagtaa taacacaaga
    52921 ttgactaata acagaataat aaatttatta tccgttacgc ccataatcaa cgaactaatt
    52981 gcctgaggaa taccggtaaa cgccatggcg aaagacatca tggaagaagt agccaataag
    53041 aacatgatcg tccctgacat tgctactgat tgaaacaaca tattaggtat atcttttagt
    53101 tttaccgttt tatagtaaaa catagcaatc aacagggagt aaatgacagc tatcgctgaa
    53161 gcttctactg ctgtaaatat ccccgtcaag ataccaccga taatgatgac aatcaataga
    53221 aggctaggaa tcgcctcttt tattattttt ccagcatgag ccatctgtcc tttttcgaca
    53281 acagggtatt tattcttttt agcaacgatg aatgctacta gcatgattcc tagcgcccac
    53341 aaagcgccta cgagataacc tcccataaac aaagcagaga tcgaagcacc accagcaaca
    53401 agtgaataca tgataaaagc agtacttgga ggaatgacca tacctgtcgg agctgacgcg
    53461 atattgaccg ctgtcgcaaa cttccggtca tatccttctt ccacttgctg agggatcaac
    53521 actccgccaa tcgcggtaga agcggcaatg gccgagctag agatggaacc aaataaagca
    53581 ttccctaaca cattcgtatg agccaaagcc cccggcacgc gcccgacgaa caacatcgca
    53641 aaatccacta gtttcttagc tatccctcca ttattcatga taatgcccga gagaacgaaa
    53701 aacggaatag cgatcagcga aaaactattg atacttccca ccattttttg agctgaacta
    53761 aaagcagaaa catcaaacgg tataactaac aataaagtca tcatcgatga gaatgcaata
    53821 cttatagcaa tcggcatccc taatacaagc aacacaacaa atacaaaaaa taacactaat
    53881 ccggcttcta ttgccatttt attcctccat ttttatattt tttcttgaat tgctggcttg
    53941 ttgcttaatg tatcgattag attgatcagg ctatagataa tgatgagtac ccccgataca
    54001 ggtactgaca gataaacata tcccatagga atacttaaag aaggcgagat ctgccccatt
    54061 gtcaatgtga cagcattcca tccgccatac accatgatca tcgcagcaaa taccagaaat
    54121 aaactttgaa caacaacttc taaaatttta ctttttttgc cagttaattt ttcacttaaa
    54181 accgttacgg ctaaatgtgc tttttgtccc acaacatacg cggaagacaa catggcaaac
    54241 cagacaagac cgaatcgaac aagctcttcc gtgaccgtat ttggatttcc taaaaccgtt
    54301 ctggaaaata cttgccataa aacaacagcg accatcccaa caagcaaaac agctcccagg
    54361 atctctaata ccttgtttaa aatcattttc atcacagcta cgctccttgc tcttggatct
    54421 tttgataaag gtctttgtag gtaggatcgt ttttgaatat atcatgaagt ggggcaactg
    54481 cctctcgaaa cggtgtatta tctacatcat tgaattggac tccatgttct tcttgtgcta
    54541 aagctttttc ttcttcaatt gcttttgcaa aagcttcaat ttcaaaaacc gttgattctt
    54601 tcgctgcttc ttcaatggct ttttcctgct catcagttag ctgttctttg atggcatcat
    54661 tcatgatgat gatatctggc acacgtgtat gctcatcata ggaatagtat ttagccacac
    54721 caccatgtcc agctgtatac aagacaaatt cattgttttc tgatccgttg atcaaattgg
    54781 attgaagaga cgtgtacact tcatcacttc ccataggtac gggcgaaccg cctaaaagct
    54841 ctaccatttt gatagcggtt tcgctttgca tcacacggat cttctttccc ttcaaatctt
    54901 ccggcgtatg gatcggaccg tctaccatat aaaagctgcg ctgaccagaa tcataatagg
    54961 tcaatccggt aaatcctaaa tttttggtcg attggtagat tggctccatt atttcttcat
    55021 tatccatcac cttaaagaaa tgttcttcat cattgaatag ataaggaact gaaaaaatcg
    55081 aataatcttt agagaaactt tccaacgctg aggcacttac ttttgtgaaa tctactgctc
    55141 ctgactgtgt caattcgatc agttcacgtt ctccacccag ttgtccatct gggaaatact
    55201 ggatctgtac ggaaccatct gttttttctt ttactaaacg cccaaattca ttcattgcat
    55261 caattactgg ctggctgttg cttgcataag caaagcgcaa ggtaactatc tcatttacat
    55321 ctgcatctgc gacagttgcc acactagaat tatttccgca gccgcttaga cctagcagac
    55381 ctaggctaag cgaacttgct gcaaacagca gtgatgtttt tttcatatgg gtttcctttc
    55441 ttttaacaat aagagacgag caattctgtc aaacaaagca ccgttaacga ctgtccataa
    55501 ggcattgctg tgattccgat attccgataa aaatctaaac tgtcccccat tcctgtcccg
    55561 attgaaacgt tttgaacttc tcctttttca tcgatttgtt caagcaatcc ttggatagca
    55621 cgataagcga cttctttata ttcttgtggt aaataacgtt tatggaccgc tttcaaaata
    55681 ccgtaagcaa atcctgcagt tgcagaagat tctacgtaag aagaaggatc atccaacaac
    55741 gtatgccata aaccgctttc agactgataa gtttttaatg cacgcacttg tgcttcaagt
    55801 gtttcgatca ataatttgcg caatgcatct tctttcgata attctaagat ttcaatgatc
    55861 tctggaatag caatcgtcaa ccagcagttt ccacgagccc aaaatgcttc ggcatagtta
    55921 tgatttcctt caaacgtcca gccgtgatac cacaatcctg ttcgtttatc catcaagtat
    55981 ttgatatgga tcataaactg gtaacgagct tcttctaaat attctgggcg attcaacagt
    56041 ttcccgattt ttgccaaagg aagcactgtc atcatcaatg tatcgtccca caactgattc
    56101 ttattttccg gtccatatgt ggcatgctgt aacccgtctt catttgtccg aggcatgtca
    56161 ttcatgaccc attctgccca ttgttctaaa taaggcaaat agcgtgaatc tttcgtgtct
    56221 tcatacaagt aagccatagt caatagcggt gccattgtat tgacgttttt gggcggagcg
    56281 ccttcttcca tacgatcctc aaaccattca ttgaccactt gataagcgcg aggatttttt
    56341 gtcatctggt aatttttata aataccatat agaccgacac cttgtggcca attccacact
    56401 gtccagcttt tgtcatctac tttcagtcca tcaaagtcca acaaaaattc accattatcg
    56461 tctttgatcg ttgtcaggtt ttcaacgagc aggtcaattt tttctttcac taattcttta
    56521 ggtacaaaaa tctcttcttt aataatcatc tttacgccac ctttttaaac gctttcattt
    56581 tatatcctta atatagcagt aataccgtcc actataaata tcgctttttg aacaaaaact
    56641 gtctaaaatt gacctaaatg ttaaataaca aaagtaactt tcagttactt ggaaattaaa
    56701 aaaagaactt ggagaaacaa cgttttccaa gttctttttc atctattttc ttatcttttt
    56761 tctacgcttt cttttgctta tccgttgtaa gcatcaatga tgatctgttt caattcagag
    56821 atcaatggtt ctttcggatt agctgtcgta cattggtctt cgtaagctaa ttcagccata
    56881 cggtctacgg ttgtatctag tgtttcttgt gtgactcctt gagcttttag actcatgttg
    56941 atgccgacag attttcctaa ttcataaaca gccgttgcta aagcttcgac taattcctct
    57001 gttgtttcgc ctttcagacc taagaatttt gcgatatctg cgtaatctgt atctgcacgg
    57061 aaatagtcat atttagggaa catcgcatgt ttttgtggat cttttgcatt atagcggata
    57121 atatgcggta aaagaatcgc atttgtccgt ccatgaggga tcccgtattc cccgccgatt
    57181 ttatgtgcga ctgagtgaca aatacctagg aatgcgttag caaacgccat accagccatt
    57241 gtagaagcgt tgtgcatttt ttcacgtgat tccatatcag gtgtttttac tgatttttct
    57301 aaatagtcaa agactaactt gattgcttgt aggctcaatc cacgtgtata atctgaagcc
    57361 atgactgaaa cataggattc gattgcatgt gttaacacat ccatcccggt atctgctgtg
    57421 acagatgctg gaactgacat gacgaattga ggatcgacga ttgcgacatc tggcgtcaat
    57481 gcataatctg ctaacggata tttcacatgt gtttcactgt cagtgatgac cgcaaatggc
    57541 gttacttcag aaccagtccc tgatgttgtc gggatacata cgaattgtgt tttttctggt
    57601 ttgtcgatct tatatgtccg tttacggata tccaagaatt tttgttttgc accaaagaaa
    57661 gatgtgtctg ggtgttcata gaacatccac attcccttag ctgcgtccat tgcagaaccg
    57721 cctccaagag cgattactgt atctggcttg aagtctgcca tcagtttcgt tcctgcgtat
    57781 acggtatttg tcgatgggtt cggttcaaca tctgagaaga cttcgatctt cacatcattt
    57841 ttacgtcgtg ccaatacttc acgaactgta tcgcagtaac cgaattggac cataccaggg
    57901 tcacaaacga tcatgacacg ttcaacgttt tccattttct ctaagtacaa tagagagttc
    57961 ttttcaaaga agatttttgg cggtaatttg aaccattgca tgttatttct ccgtttcgct
    58021 acagttttga cattgattaa gtttacagca gagacgttac gagaaacaga gttcttacca
    58081 taagaaccgc atcccaaagt caatgacgga atcatttcgt tatagatatc accgatgccg
    58141 ccttctgcag atggtgtatt cactaaaata cgacaagctt tcattcgaag accgaatttg
    58201 acatgcaact cttcgttttc tgaatggata actgctgtat gtcctaatcc tccaagattc
    58261 aacatacctt cacacaaatc aaacgcatgg tccgtgttgt tagctttgat catggcaaga
    58321 actggagaaa gtttttctct tgatagcgga taatctgggc ctacgccttc caattcagca
    58381 atcagcattt tcgttccttt tggtacactg attcctgcca attttgcgat ttccatcgca
    58441 gaatgtccaa cgattgatgg attgaccgca tattttcctt cgttcatcac tgcatcttct
    58501 aatttttgca attcatctgg ttttacgata taaacttggt gtgcttggaa ttctgctttt
    58561 actgctgcat agatttcttt atccacgatc acagcttgtt cagaagcaca aatcattcca
    58621 ttatcaaatg ttttcgaaac gatcaaatcg ttcactgcac gtttaatttt tgctgttttt
    58681 tcaatataag ctggtacgtt acctggacct acacctaaag ctggttttcc tgtagagtaa
    58741 gcagacttga ccattccagc tccacctgtc gctaagacga tcgcgatacc tggatggttc
    58801 attagcatag aagtcgcttc gatcgaagga tgttcgatcc attggataca attttcagga
    58861 gctccggcag cgatagcagc atcacgtacc actcgtgctg cttctgcaga agatttttgg
    58921 gcacttggat ggaaagcaaa aacgattgga tttcttgttt tcaacgcgat cattgatttg
    58981 aaaatcgttg tggaagtcgg attcgttgtt ggagttactc cgcaaaccac accgactggt
    59041 tcagcaattt caatcaaccc tttttggaca tcttcattga tcacaccaac tgttttatca
    59101 tgtttgatat tatgccaaat atattcggac gcatacatat ttttgattgc tttatcttcg
    59161 taaacaccgc ggcctgtttc ctctacagcc atcttcgcta atggcatatg ttgatccaaa
    59221 gctgccattg ccatttcatg cacgatatgg tctacttttt cttgatcaaa gctttccatt
    59281 tctttcaatg ctgcatttgc tttttctgcc aaatcatcaa tcattgcttg tacgtcaatt
    59341 gtcttcgttt tttctttttc tattacttta gccatcatta tcctcctaat gggtcatggt
    59401 tatttgttaa ataattcaca tgtatatcat acaatattta tttttctatg taaacacttt
    59461 tttgtgattt ttttcacagt tatttttaaa tataaaattg ctttatttac aggtgtttgt
    59521 atagaaaaat atataaagtt atttattatt atttatatga cacacccgat tattcaatat
    59581 tgagcaatat atcccatgct cttatctctt tcttttttga ataaaaaatg aggtaggaca
    59641 tctttctgcc ctacctcgtc tttaaataag ctttgatact tatttttctt ccttctcttc
    59701 tgtctcttcc agcgtttctg ttgtttcagt ttttacttca actgtctcag ctggttcttc
    59761 tgttgtttca acagtagtcg ttgcatcatt tgttacgaca gtccctggtt tgaccgtttt
    59821 gattgctgca cgatcgtact caaggaagat cccttcgcag tctaaaacaa ctgtccgttt
    59881 gtcattgtcg atttctgaaa caactccatg taatccgccg atcgttacga ctgaatcacc
    59941 tggtttcatg ctatctaaca agttttgacg ttcttgctgt tgtttctttt gcgaacgtga
    60001 cataaaaaac cacattgcta atagcgcgat aaacatcaca agcattggta atgttcccat
    60061 ttttttcacc tcactaattc tgtatctaca aaaaacactt tagcagaaaa atgctgataa
    60121 cactagtttt ttacttaatt tttaaaagat tttcctttag aagctttttg cgttttcttt
    60181 attgaacccg tattcttcaa aaaaggcttc tctaaattca agcaaattat catccatgat
    60241 tgcttgacgt acttgtttca ttagattcaa caagaagtat aaattgtggt aagaagtcaa
    60301 acgaatccca aatgtttcat cacatttgat caaatggcgg atataggcac gtgtatagtt
    60361 tttacatgta taacaatcac atttctcatc aattggacgg aaatcatggg catatttcgc
    60421 atttttcact actagacggc cttgagaagt catacatgtg ccatttcgag cgattcgagt
    60481 aggcaagaca cagtcaaaca tgtctactcc acggataaca ccatcgatca atgaatcagc
    60541 agcaccgaca cccatcaagt aacgtggttt gttttcgggg atcaacggtg tagtgaattc
    60601 taatacacga ttcatttctg atttaggttc cccgacagat aacccgccga ttgagtaacc
    60661 agggaaatcc atcccgacta gatctttcgc gctttgacga cgaagatctt caaaccctgc
    60721 tccttggatg attccaaata atccttgacg gtctggatta gcatgagctt tcaatcctcg
    60781 ttctgcccat cgagacgtac gttctactga ttttttcacg tagtcgtaac tttcatcgaa
    60841 tggtggacat tcgtcaaaac tcatcatgat atcagagccc aatttatttt ggatgttgat
    60901 tgctttttct ggtgataaaa acatacgcga accgttcaag tgatttttga aatgcacgcc
    60961 ttcttcttcg atattccgca tatcacttaa tgaaaaaact tgaaagccgc ctgaatctgt
    61021 taaaatcggt tgatcccagt tcataaattt atgcagtccg ccagcttctt ctactaagtc
    61081 ttcaccaggg cgtagccata aatgatatgt attacttaaa ataacgccag cacccatttc
    61141 tttcaactct tctggggaca ttgtttttac tgtagctaat gttcctactg gcatgaacat
    61201 tggtgtgggg aaagttccgt gtggcgtgat caattcccct aaacgcgcgc cagtatgttt
    61261 ttctttcttg attaaacgat agcgaatggc aggttgcgtc attcatttcc ctactttcta
    61321 tataaaatat cttgacaact cccaccatca taatcgtttc aagtgctttt tgcaagttcc
    61381 ctcggcttcc ttctacttta tttaatattc ttttttttat ccttttttta tgaataacaa
    61441 aaaaaagaaa aacattatga cagaaatatt ttttgataca tggtatattg gtgttattaa
    61501 agaaaaggag ggaaaaatca tgcaatcttc tgcagaatta aaacgccaag caaaagagag
    61561 tctaaaagga cgttggggac aagctgtttt actaaatcta atccctacat tgattggtat
    61621 tgctactatt cttcttatta tgattcctat tatcttgttt gccaccacca tatatgatgg
    61681 atcagcgagc gtctcagaag cgacaacttc aggaggcggt agttcaggag gcggtggcgg
    61741 aattatttcc agcatcatta gcgctttgtt catgagcggt atttcttgga cttatcttga
    61801 tattttacgt ggacaaaaag atcagatcga accattcaaa gatgcatttc gtggattcaa
    61861 aggtttgttt atcggcggag tgatcagctt agctgtgctg atctcgatct ttacaacatt
    61921 atggggtttg ctattagtta ttcctggaat cattaaaagc tattcttatt ctcaaagcta
    61981 ttttatttac tacgatatcg tccaagaaac aggacaaaca cctaaagtac tggatacgat
    62041 cactgcaagt cgaaaattga tggatggtta taaaggacaa cttttctggt tagatctttc
    62101 atttatcggc tggcatatct tagcattagc aactgctggt attggttatc tatggctcag
    62161 cccatacatt tctgcaacaa aagcagcatt ttatgaacaa ttgccaaaag atatttaaaa
    62221 gagagtgtga cttgtgaccg agggggtggc aggagaacac cgtttatcga gaggcagtaa
    62281 aagaaacatt cagcccatta gcaaaaagga ggtgtgacaa cattcatgtc acacctcctt
    62341 tttgtatagt tccagtaaaa agaaaatcaa cttagaaaca gttaatcatt cctcttcttc
    62401 tgcttccggt aatcgatgga acttgatcag ttcattcacc gcgattggta cgatagataa
    62461 aatccctacc aagaaccagt gattcaaact gatgtgtact aggccgaata gttcttgcgt
    62521 aaatggcagc aaagcaacag ccgttgtaat agctaatgcc agtaacgcca tttctaccaa
    62581 agctttattg gtcgctaaat tgactttgaa gatcgattgg ctgctgcgta cattgaacac
    62641 atgcagaatc gatgaataag ctaagatcaa gaatgcaaca gtttgaccga catgctgact
    62701 tgcgtctacc catgggctga tagtatcgac ataactacct aaataataac caaacaaggt
    62761 tacgatggtg aagacccctg catttatggc aattttttgc aataaacctc gagcaaagat
    62821 cccttcattt ttcggaatgg gtggctgatc catgatgccg tgttccgctg gctctcttcc
    62881 taaagcaaat cctggaagtc catcagcaac tacattgaca aacagtagtt gcacagctgt
    62941 aaatggtgct ccccatccta acagcatcgc aatcaaaaca ataaagatct ccgaaatatt
    63001 acagcttagc aaaaagttga tagctttacg aatattctga taaaccgaac gtccttgact
    63061 gacagcgtct acgatcgtcg caaagttgtc atccgtcaaa atcatatctg cggcactttg
    63121 cgccacatct gttcctgtga tccccatggc acacccaacg ttacttgctt tcaacgctgg
    63181 tgcatcattc acaccatccc cagtcatcgc tacgactgca cccgtacgtt gccaagattt
    63241 gacgatccgg atcttgtctt ctggcgttac ccgagcatag acagagagag ctttgacacg
    63301 cgcatctaat tcttcgtctg ataattgatg cagttctgat cctgaaagtg cttctgacgg
    63361 atcattcaag atacctaatt ctttcgctat ggcactggca gtcaccacat gatctccagt
    63421 gatcatgact gttttgatgc cagccttttt cgctcgcttg attgctcctt tactttctgg
    63481 acgtggtgga tcgatcattc cgatcagacc aatcaaacgt aagtcttttt ccagtgcttc
    63541 agaagaaata tcttgcggtt cttcgtcata agtagcataa ccaacagcaa tcactctcag
    63601 tgcccgttta ccaaagcgat cattaacgat cgccgcttgt tctacatcgc ctgaactaaa
    63661 gcgcggagca agaacatcga atgctccttt ggtgattgag atgtattttt cgcccatttg
    63721 gtgaactgtt gtcatcattt ttcgttcaga atcaaaagga agctcgttta ctcgtggata
    63781 tttttcttct aattctgcct ttgtatgata attctcttca acagccctta cgatggctac
    63841 ttctgttgga ttacctttga taacaaggtc gtcatcttct tgctcgaccg tcacatctgt
    63901 acatagtgca gccattttca atatttccat cgcgctgttc gtcattgaat cttcgatatt
    63961 cgttacttca tcttctttgc tccatacccg gcggacgcgc atttcatttt gtgtcagcgt
    64021 tcctgttttg tctgagcaga taacattagc ggttcctagc gtttcaactg ctggcaactg
    64081 tcggatgatc gcatgtttcc tcgccatctt ctgcacaccg tatgcaagtg tcaacgtaac
    64141 aataacagtc aaggtttccg gtacagctgc aacagccaag gaaatcgaag tcatgaatat
    64201 ctcaaggagt ggttctcctt gcagttcacc aatcacaaat actaaagccg cagccgctaa
    64261 agcgatcata ctgattcgtt tgcctagctg attcaatcgg acctgtaaag gtgttttttg
    64321 catagtattt tcgttcaaaa gatccgcgat cttacccatt tctgtagcca tgccaatggc
    64381 agtgacgatc gctttcccac gacctgcagc aaccgtacat cctttaaata ccatattaag
    64441 ttggtcacct aatgactcat cttcttcagc aataaatgct gcatcttttt caactgcttc
    64501 actttctcca gtcaacgccg attcttctac acgtaattgt gatgcttcga tcaaacgggc
    64561 atcggcagaa atagcatcac cggactctaa aacaagaata tcacccacta ccagttgctc
    64621 tgactcaaca gtggtcactt tgccgtctcg tatgacagtt gccgtttgtt tattcatatc
    64681 ttgcaaggat tccagtgctt tttctgcgtt tccttcttgg acaatcgcca ataccgagtt
    64741 gatcacaacg atagcgatga tcagcagtcc ttcaaataaa tccccatgtt ctgtagcaaa
    64801 tgcggtataa aaggaaatca ccgctgctac aagaaggatg atcgttgtaa aatcggacaa
    64861 gcttcgaaag aattttttga tcatcgattc tttcggcgcc tcatcgaatt tattcaatcc
    64921 ctgctttttt cgccgttcat ccacttcttg tgaagacaac ccagtatctg gatctgcttc
    64981 tgttaccttc tttacttctt ctattgtctg cttgtattct tccatcctat tcctccttta
    65041 aaatcttcgt acaaaaaaag gtacttattt tactataaca tccttttttt atttacttca
    65101 aataaagggc ttatcgatta tttgttaaga aagcgtaaaa tttaaagtgc ttatatttgc
    65161 aaacgctacc aaaggatgct attatataga tgtaaggaag gaggatacca tcaaatagac
    65221 agaagaattt ctcgatagaa cagtgtaagc aaacaaagtt gttagaagta caggtttcac
    65281 ccagacatgc gtgtagagta caaacctata aatagtgaat gcgtatggat gtttggatca
    65341 cgaagtatcc gaaacaagaa gtaatgcgat acgtaacaat tgaataacgt aaacgtctga
    65401 aaattgaata ggaacaagag atgtttcaaa tgttgaagat gtaacgaaaa caaaattttt
    65461 gtttaacatg aagaaattca acaccgtgct ttgcatgtat gatggaaagt tacaatcatt
    65521 tctgtttatt tgaatccttc cgaggtacaa gaaagccgag cttatcgaga gctcggcttt
    65581 ttctttgtca taaaatcttt gtaaagcttt tttgttattc aaaactagcg gcttttcata
    65641 accttatttc acgaacatcg catctccaaa actaaagaaa cgatagcgct cttcaatcgc
    65701 atgctggtaa gcagataaag tcaattctct tcctgcaaag gcactaacta acatgactaa
    65761 agtcgatttt ggcaaatgga aattagttga gaaagcttct accactttga attcatagcc
    65821 aggcgtaatg aaaatatcag tccatccgct gtctgctttg atttctccat taaatttcgt
    65881 tccaatcgtt tctaatgttc gaattgaagt tgttccgaca gccacgattt tcccgccatt
    65941 tttacgtact tggttcaatc gctcagccgc ttcttccgtc aaacggtaga attcactatg
    66001 catatggtgt tcttcaattt tatctacgct gacaggtcgg aatgtaccta accctacatg
    66061 tagtgtcaga tagactaatt caacaccctt tgcttcgatc tcatcaagta attctttcgt
    66121 aaaatgcaat ccagctgttg gcgctgcagc agaaccattt tctttcgcat agacagtttg
    66181 atagcgatct gggtcttcca gacgttcttt gatatatgga ggcaatggca tttctcccaa
    66241 tgattcaaga atctctagaa aaattccttc atactcaaag tcaatgatcc gtccgccgtg
    66301 ttccaattct tctttcacta ctgcttgcaa tctgccatca ccaaaagaga ttcttgtccc
    66361 aactttggca cgtttcgcag gtttgatcaa tgtctcccac gtatctcctt gtgtattcgt
    66421 tagaagaagg acttctaaat gcgcaccagt atctggtttt tctccatata aacgagcagg
    66481 cagaactcgt gtattattca taactagcgc atcaccaggc tgtaactctt caatgatttc
    66541 atggaaatgt ttatcttcca tctctccagt tttccgatcc aaaacaagta aacgagaatg
    66601 atcgcggtct tttaatggtg tttgtgcgat taattcttcc ggcaagtcaa aatcaaaatc
    66661 ttctgttgtt aacatatcgt cactcacttt ctatcatgtg cttcatcatt ctatcatgtt
    66721 tttcgttctg ttggcaaaat cagcactttt tcacttctat tttttgtttt cctaaataaa
    66781 aaaactaccc tatttgtatt ttttcattgt tttaggcata ctagtaatag atagttcttc
    66841 attgaaaata gaaaaagagg tggcaccatg attgaatttc aaaatgtgtc aaaaatttat
    66901 aaaggtggaa aagtcgctgt agaggacgtc aatctttcat ttgaaaaggg tgagttcatt
    66961 tgttttatcg gtacgagcgg aagcggaaaa acgacgacga tgcggatgat caaccgaatg
    67021 actgatccaa cacaagggaa aattttgatt gatggaaaat ccatccaaga aattgatcca
    67081 gtggaattgc gacgtcagat cggctatgtt atccaaagta ttggattgat gcctcacatg
    67141 acgatccgtg aaaatatcac tcttgtccaa aaattgttga aagtcagtca agaagaacgg
    67201 aataaaacag ctgaaaaaat gattgatctt gtggaattgc cccgtgagat gttagatcgc
    67261 tacccacatg aattatctgg tggtcagcag cagcgtattg gagtcgttcg tgcccttgca
    67321 gcgaatcaag atatcatctt gatggacgag ccattcggtg cgcttgatcc aatcactcgt
    67381 gattccttgc aagaccttgt caaagattta caagagcgtt tgggaaaaac gatcgtattt
    67441 gtaacccatg acatggatga agcgattaaa ctagcaagcc gtatcgccat catgagtgaa
    67501 ggccgtgtga tccaatttga tacaccagaa aatattttac gtcatcctgc aaacgatttc
    67561 gtagaagagt tgattggaga agatcgccta ctacaagcca agccaaatgc gacaagagtc
    67621 ggcgaagtta tgctaaatac agcaatcacg attacaccgg aaaaatcatt acaagaagca
    67681 atcaaactga tgagggaaaa acgtgtagat acgttgttag ttaccgataa ttcgaatgta
    67741 ttaaaaggat ttatcgatgt agaaacgttg aataaaaaac gaggaaaagt atccagtgtc
    67801 ggcgacattt tgaataaaca tgttttttac gtaaaagaat ccgcctatct tcgtgatacc
    67861 ttgcaacgca ttttaaaacg cgggctgaaa tacgtgccgg tcgttgatga acagaacaaa
    67921 gtcgtaggta tcctgactcg tgcatcatta gtggatatcg tctacgatgt gatttgggga
    67981 gaagatcccg caactactga tgttgcagag tcagctactc ctgaagaatc taaggaggtc
    68041 tgacctatgc agcagttttt tcatcagcac ggttcagaaa ttctctcaaa aggttttgaa
    68101 catctttata tttcaggact tgctttgatc attggtatca tcattgctgt accgcttggt
    68161 gtcttactga cacgttttcc aaaagctgcc acgattgtga ttggcttaac cagtgccttg
    68221 cagacaatcc cttctcttgc tttattggca ttgatgatcc cgctatttgg cgtaggtaaa
    68281 ttacctgcga ttatcgcact ctttatttat tcattattgc cgattttacg taatacgtac
    68341 attgggatga aaaatgtcga ttggaattat cgggatgtgg caaaaggcat ggggatgact
    68401 gagttccaat ccattttttc cgttgaattg cccattgcta tgccaacgat catggctggt
    68461 attcgtttag ctgccgttta cgtgattgca tgggctacac ttgcctctta tatcggcgct
    68521 ggtggtttag gcgatttgat ttttagtgga ctgaataatt accaaccaga tctcattttg
    68581 gcaggtacga tccctgtaac aattttagca ctattggctg attggcttct aggtctcttg
    68641 gaacaccggc tgacaccgat cgcacttaga gaggagaatg aagaatgaaa cgtatactaa
    68701 aaatgaccat ggttcttttc tctcttctta tacttgcgag ttgttccttc cctggtcttg
    68761 ccagcaatac agatgaagac accatttcga tcacaggtgg aatcacttca gaagcacaga
    68821 tcttagcaag tctagttgcc ggaatggttg aacattatac agataaaaat accgcaatca
    68881 tcaacaatct ggctactaca actatcaacc atcaagcgat gatgaacgga gatgcttcaa
    68941 tttcagcggc tcgctacaca ggaacagatt taacgactac attgaacctt cctcctgaaa
    69001 aagatccgaa aaaagcgttt gctaccgtga aagatgaatt tgagaaaaga tacggtcaaa
    69061 cgtggttccc gtcctatagt tttgaaaata cgtatgtctt tcttgttcga aaagacaccg
    69121 cacaaaaata ccatctatct aaagtcagcg atctgaaaaa tgtagcggat gaattagtag
    69181 ctggtgtaga tacttcttgg atcaatcgta aaggagacgg ttatgacggc ttccaagaaa
    69241 cttatggctt ctccttcaat tcgatattac ctatgcagat tggacttgtt tatgacgcag
    69301 tcgaagcagg aaaaatggat attgttcttg gctactctac ggatggacgg atcgccagtt
    69361 atgatctagt catgttggaa gatgatcgtc aattcttccc accatatgat gctgctccag
    69421 ttgtagacaa caagatatta gctgatacgc ctcatttaaa agaagcctta aataagttag
    69481 gtcataccat cagtacggaa aaaatgcagg aattgaacta cgaagcagat aataatctag
    69541 tggaaccatc agtagtagca caacgtttct tacaagaaaa ccactatttt gaagggaagt
    69601 gaagcaattg gataaaatga atgtctttca acaatttttc tattatttcc aggaaaatgg
    69661 ctcctatatc tttgcacaat ttatccgtca cttcctgatt tctatctatg gtgtcctctt
    69721 tgcagcagtt gtcgggattc cggttgggat catgatttct aggagacgta aattagcaaa
    69781 ctgggtgatt cgtttggcga atatcatcca gacgatccct tctctagcca tgatctccat
    69841 tttaatcatt ggattaggcc tgggagtcaa tgtcgtgatt gttactgtat ttttgtattc
    69901 cctcttaccg atcatcaaga atacgtatac cgggatgatc caagtagaca aaaacatcct
    69961 tgatgtcggc aaaggcatgg ggatgactgc tagacaacga ctattcatgg tagagttacc
    70021 actctctgtt tctgttatca tggcaggcat aagaaacgcg ttagttgttg ccatcgggat
    70081 aaccgcgatc ggtgcattcg tcggtgctgg tggtcttgga gatatcatta tccgaggaac
    70141 caatgcaaca gagggcacat cgattatttt agcaggtgca ttgccaactg cattaatggc
    70201 gatcatcacg gactggcttt taggtatttt ggaaagacgg ctagatccag ccagccgtac
    70261 atcacgctga tgttattgta aaaaacaaaa gcttctgcga ccatttgttg gtcgcaaagg
    70321 cttttttagt tctgtcattt ttaaggcaat aatccactct gattcttccc tccttatggg
    70381 caaagagttg cttttcttag ctatttatcc attccgtata ctccaacttc atactgccaa
    70441 cgggttcata tctgcatcca ccgactaagt ttttttctta tccgctaagg cattttgcca
    70501 atccattttc ccatcactcg tcatggaatt cataccacat gatctctaaa tactcgtctg
    70561 cttgttcttt gaattgggca tcaattctta catcctttgg cgtggctttc atttaagcct
    70621 tttccatcag tcctcgattg ttcgatgcct tcagctgcat catcaaaact ggtgactgcc
    70681 gtatcggtaa cttcgatcat cttttataca atcgggaact tgccatcctc atccttggct
    70741 aaaaacatat ctagccattc cagcttctcg ccatgggagg tttgcggttc ctaaaaccgg
    70801 tacacgattc ttatggactg catccgtcgc aactagactt ggcgatacga tgatccttca
    70861 ccagaagagc acccccgtaa accaattgat cgatatattg ccaataagaa aagacattgg
    70921 cgtaaaagat atcgatccct cgtgatgcat tgccgttgat actgctattc atggccgaaa
    70981 gcgccacgac tttaacttct tccgtttgag tgacattcat gttcggcaat gtcgctttgg
    71041 atacccgctc tgctaaagga acgacactgg tattgtattt cgtatctgta tcttttccaa
    71101 aagaccatgc caacgaatcc tctgggaacc aataagacga ctccggttga ttgtccattg
    71161 cttttttcaa catacttttg gctgtttctc gataaacgac atttccaatt gtcttcgcct
    71221 cactttggcg ataatcgatg gtcgttgcca ctgccaacac taacaacgat gctccaattt
    71281 tactgatttt ccttgtcaca cttcttcctc cttgttttac ataaacccgt caacattcca
    71341 ttcctaaact caaacaatga cccttcaaag gtggagatat aggaacaaat taaagaaatc
    71401 atgcaataaa tccaagaagg ttggttttag aacaaaaaaa gagtctcctt tttggggaga
    71461 ctctttgtta actatctaaa aaggttggct cttctcactt cactcataat gtgtccaggc
    71521 agaataaatt tctacaactt tattttcctc atcaactttg taaaccactc gatgctgtct
    71581 attcagcctg cgtgagtatc tgccttcatg tgtaggtctc agcttttcaa aagactgcgt
    71641 gggcatatat ggatcttcct tcaatgtctg aatcacttct tcaaattgtt tctttaaatt
    71701 cgtttgcttg atttttctta aatccacttt cgcagagttt ttgattgcga ctgtatagtt
    71761 actcatcaaa gagcttccca atcaatatca tcgatattcg taaagctact gtcgtctttt
    71821 tcgcggtctc gtactttatc taaagtacct gtttgctcaa gaaaaagggt ttcttgaatc
    71881 gctctccagt cttccagccc aatcaataca gcattgtttc cactacgatc actaataatc
    71941 tcgatttcag tatgattttc atttacctct ttcaataact gataaaaatt tttacgagca
    72001 gtgctaggat tcatcgttgt attagccatt gtctctcctc ctttttctga tataattata
    72061 gcgtacttga ttaagtacgt caaaaaaata caataaagca cttaaattca gcaaactttt
    72121 cctctttttt acatagagaa aaagacccct ttttagggag gatctttgtc cacgctcact
    72181 tcttactcct ttacgactag cggtagccaa acgtaaagta attggtaaca acgtatcatc
    72241 tttagcaaag gtcgaatcca aagcctgtga gtctatgcaa caacaccaat acacgaaggt
    72301 tcctcgttgc agtgtgactt gattgatatc ttgaataacc tgaggatacg cacgaaagat
    72361 ttgtaacggt tattcaaaac tgattttggc cgtcaagtct tctactgcct caaaaatgat
    72421 tgcctctttc gtctcctgtc attcatgttc actatcgatt tgatacttgc ttagaccgtt
    72481 gggtttgtga atcaccaatt aaaaatggca ctgagttatg gtaacaaaaa atggtcactt
    72541 gacgaggctg agattcctac ttatgaagac acatggcact taagagtcga ggtaaaggaa
    72601 gaagctgaaa atataggagg ctgtacatta tttggcaaag gattcggaaa ttatgatcaa
    72661 gacatcaaaa tcaaaatgtt ttacagctaa ataggaagaa taataacatt tcatacgctg
    72721 ttcccatctt taatggctta tagagggcac ctttagctga gaataagaca aaacagaccc
    72781 tctagcttca attaagagct agagggtctg tttttgattt taaaacttgg tttatttctt
    72841 caaatggtag ctataggtgc tgacaacgat attttcacgc caagacaatc gcaatcttaa
    72901 ccgcaaactt gtttggttat ggagaatatt ctgccagcgt ctgtttggta caaattgagg
    72961 aatcaacaca gtagttgtat agttttgttt cgctgcattt ttacttacga tatctacata
    73021 acggataatt ggattttcaa tcgagcgata agaagagtgg acgattgaaa aacggacatc
    73081 tgggaaatgc tttttaaatt ctgcttcgat ttctttttct ttttcgacat cttcgtctag
    73141 cgaaacatgc atcgcaacga cataatcacc gatcgaacgt gcgtaattca acgcaccgat
    73201 atttacccgt gtcacattcc cgactaatac aagaacggta ttcccgtcat aatcatgtag
    73261 ttttgcatca ttttctaaac gaagctgttc tgctactttt ttataatgat catggatctt
    73321 ataaaaaatg aacataacga ttggcataat gatgaagaaa ggccaaatgt cccctaaccg
    73381 atagacaaac aaaatcgcaa tgattgcata tgagatgaac gcacccgtaa tattggcaat
    73441 cgatttgctt aaccatcttt ttccttcttt tttccatttg acgaccatcc ctgtttgaga
    73501 taaagcaaat ggaatgaaca cgccgataga gtataacgga atcaagcgtt ctgttgagcc
    73561 atggaaaata aacagcaata cgatcgaacc tagcgctagt gtgataatcc cattggaata
    73621 gcctagtcga tccccacgat cttgatacat gtgaggcatg aatttatctt ttgccagatt
    73681 gtatgcaaga actggaaaag cagaaaaacc ggtgttagca gcaactgcta aaatcagcgc
    73741 tgtagcaaat tgaaggacgt aatacaaaat accatgtcca aagacagctt taccaatttg
    73801 agacaaaaca gtgacttctt tttcaggaac gatcccatac caatagttga taaatgtgat
    73861 ccccacgaag aagaaaccta agatcaatgc catcatcgtc aatgtagcag cggcattttt
    73921 tgctcttggc ttcttaaaga atggtacagc attactaatc gcctccaccc cagttaaaga
    73981 ggaagaacca gaagaaaatg ctcgtaaaat caaagcgata gaaacacccg gcacagccgc
    74041 tcctggaagt gcagtagctt gcaatggcac agcacctgtc acgatattat ataaaccaac
    74101 aacgatcagt aacgagatca caaggataaa tgtgtacacc ggaaataata ggaacgatgc
    74161 agattccctc agcccacgaa gattcagcat catcagtagc aaaacgattg tcacagaaat
    74221 agcgacttga tgaccgtaga gtgctggcac cgctgatgtg atcgcttcag cacctgctga
    74281 gacagatact gctactgtca acatgtagtc gatcaataaa gagccaccgg aaatcaagcc
    74341 agcattcttt ccaagattct cactgctgac gacatatgct cccccgccat gaggataagc
    74401 atggatgatt tgacgatatg atagcgttaa tgaaatcaat aaaataataa caaaagcggc
    74461 aatgggtagc gaataccaga tggctgcagc agacaaagcg actaaaacga caacgatttg
    74521 ttccgtacca tatgcaatag atgataacgc atcagacgac aataatgcca aagcagcaaa
    74581 tcgggttaat ttgtgctcat cattttcagc agattttaaa ggcttaccta ctaacaacct
    74641 ttttaagtaa tccaatttct aaactccttt acacacaatt ttacttcaag ttttatagtc
    74701 gaaaatttat agtaaacaag atgttatagt acgctcactg accttaaaag tcaataaact
    74761 ttaagaaaat aattggaaaa aaacagaaaa atatcaagcg ctttttcaaa cgctttaata
    74821 cagcaaaaat ctaacatttt tttatgggct ttttctttga attctataat aaaaggatag
    74881 aacttgactt agaaaatcaa gttctatcct ttttgatatc ttaacttttg gaagagagat
    74941 gtaaggctgc tttctaccca tcattcttac tactcgaagc atagcgcagt tgtcacaacc
    75001 tcttgataaa cggtgtttaa aagcttgcat ctgcggtaat aagcccaacc aaattaaaaa
    75061 cataagaagc tgtttttatt tggtcgttct tattacttga tgcagagcag cttttttaca
    75121 acctcttggt acacggtgtt caatcagctg accctcggta ttagctcaaa aaaacggcaa
    75181 aatatgagga actatttttc catttttttc tctaatactc aggtcaaaac actgatttca
    75241 caacctcttt ttttaatgaa aaatatggct ctcatgcgcc gcttctttga atttcgtcat
    75301 tttttcttcg atgaacggat agattgccgt tcctacgatt ccaaagacaa caaacaaacc
    75361 gatcatgatc cagattgctt tcgtagcatt tggccaataa attcctcctg cagattcccg
    75421 taacaagttt accgcatgag taaatggcaa gaacggattg atcatttgga agaatttccc
    75481 tgagacttgg atcggatagt tcccgcctcc gccagagatc gacagtacta agatgataat
    75541 agcgatccct ttaccgacat tatcgaataa tgcagccaag acatagacga tcatcatgaa
    75601 cgctaaggcg atcaagacag caaatgacac gctgtagacc ggattttgga catctactcc
    75661 tagtaagaaa atattcccta atgtaacgat caagctttgc gcaatcgcca ttgtcaaaaa
    75721 tgtcagcata cgagcaacga attgttctcg tttcgtaaaa ttgtttcggt ctttctttcc
    75781 taaatagtat tctgtcgtag caacacttga aagaagaaca gcgccgaccc acagacatag
    75841 tgcagtataa aacggggtgc tagctgaacc gttatttgcg attggataaa gcgcattggt
    75901 ttgtagctca acaggttgtg taaagaagtc actttctttt gttgcgtcta gtttcaataa
    75961 tttgatgact tcgcctagat cgacttcttg ttcgcctttt cggatcgcgt ctgctgcttt
    76021 ttggatacca gctttgatat ttggccagtc attgttgatc aagtccgcag cagcgttcac
    76081 agctgtttcg acttctggca ttttttcatt tactgttgtc aatgtaccgg tgatttcatt
    76141 ttttagtgct ggccaatcat tttggataaa atcagcggcc aatcctaatt tttgctgaat
    76201 gatcggtaat tcattgttgt ataggtctgc cccttcattg atgccattga cgatggtatc
    76261 catatttcca ttcagcattg tattggcatc atggatttct tgtccaattg caggcatttc
    76321 tttttgatat ttttctaaaa ttgccactgc attcgttaca gttgctttcg ttgagctgag
    76381 aagcgaatca aagtcgatct ggtccgcttt gctcagtgcg ccttgtgcag tcccaatcgt
    76441 atcgatgact tggtctagta gagaagtgac agtggaactg atttgacctg tattgatacc
    76501 agcaatgatg ccattgatat tattaacaac ggcatcaatt tgatctagca attcatcaat
    76561 agccttcgtg tcttcatttt gaacagctgt gttcaaagca gataggcggt tttgcagatt
    76621 gcttagcaag ggttttaatt gatttagctg atcaataatt ggctgtaatt tcgtatttcc
    76681 agcacttgtt tgcaatttag tcaggaactc gaccagttta tcgattgcat tttgttgttg
    76741 agatattccg tcaataaagc cgttcaatac attgctgatg tttttctttt catcatcagt
    76801 cagcttacca tcttcaatag ctgttcttac agtatctatt aacgaacctg tcgttgttgc
    76861 cacttgtcca attgacttca atgtaacatc tacactgttt gtgatgcttg gtatcgcatc
    76921 tttcagctga gaggcacctg attttgtttg gtttgctaga tcacttgcct gattttctaa
    76981 atttttcaca tctggcaaga tatcttgtac ttgttggatg atcgtcaagc cttgttttgc
    77041 ttcatcgatt ccttggttca ttgtctcttg gatcgaagca aaatcgccgt cgatctctgc
    77101 tagttgctta ccggcatttt ggatctctgg gattttttct tgcaaatcaa ggatgatttt
    77161 tgcttgatct ttcaactcag gcattttgtc attcaatgca acgacttttt cacccatctc
    77221 atctacttta gggatgtaat ccacgaattc atttgcttta ttcagttttt ccttgatttc
    77281 aggtagttga ctttgtaatt cgaggacttg ttttgtgtag ccgtcgatcg tgtccaaatt
    77341 ttcatctgtt gataaaatca tatctttaac tttattgata cttactaggt tcgtatcgat
    77401 gtcgtaacct atctcattga atactttcaa taaagtcgaa ctggctgttt tgataaattc
    77461 atttgtgatt tgcgattgga tcgaacttgc tcctttatcg gtgattttcg gtgcaatcgc
    77521 attgattttt tcatttaccg tatattcgat ttttggtttc ttgatatcac cactagtaaa
    77581 actcagcaga tcttctgaga aatcctttgg caagtaaata cctgcgtaat actttccgga
    77641 acggactccg tcttctaatt gttcttttga atcaacgaat tgccatccta attgtttatt
    77701 tttatgaagc gattcgatga cttgctcccc aatggccact tctttacctt gaaactctgc
    77761 tggcttgtct gcactgtaaa cagcaattgg cagctctcca gtatttccat atggatccca
    77821 taatgctttg atgttaaacc acgcatatag cgaagggatg atcatgatcg cgatgatcag
    77881 aagtgttgca atcgggtttt tgaaaatccg tttccagtca agcaaatata atcgaagcgt
    77941 atttttgatt gactgcatag ttctccccct tcttcttttt tcagctgaat tactttacca
    78001 tgtatttcat acttttttca atcggctttt ttcaaaaaag tcgaattttt ggacatatca
    78061 cggcaattgt ctcattgtaa tctcttacat attgagaaat aattaaggat tacagccttt
    78121 actttatcat gaaataaaag ggaaagataa ttttatgcca aagtttttat ctattatacg
    78181 tatacgcaaa aaagctaaaa cattttttgt ctttgcccct acaaaaatgt ttcagctttt
    78241 ttcgtttttt attcctgtat ttcttatttt tcgaatgaca ggatgatttt cccagcagga
    78301 ggatgtcctt caactagctg atgtgcttgg atgacatgct taagtgaagc agacagattc
    78361 attgaaataa aaacatgaaa ggctcctttt tggtaggctt tgattatgcc agcaaaggct
    78421 tctgcatcta gatattcctt tcttggaaca aacgattcat aaaagccttc ctttttttgc
    78481 cgcaaatcta gatctgggag gtcatttaat gcaacatacc tgccgcctgg tttcatgatc
    78541 tggatgccgg tttctccttt gatacttcct ttcgtcgcat cgattacaag gtctgcctga
    78601 tcagcaaatt gcagacctgg atttgtttta tcataagcag caaatgctga agctcctaat
    78661 ttttttacct tttcctcatt tttactggat gcactggtta agattcggat tcccttttca
    78721 tggagaagct ggatcagact tgacccgacc gcacctgatg ctcctagtat cattacagta
    78781 tctgtcggct ggatttccgt caaatggttg attagattat aagccgtaat tcctggcgtg
    78841 accattccgg ctgcttcttc ccaactcatt ttatctggaa tttttgctac ttttgctgat
    78901 ggcaacacga cttcttctcc gtaagtcccg ccgactgcat ggacagctac tcgatcacct
    78961 acatgaacag ttgtgacatc tgaaccaatc tctgtcacaa taccagcgcc atcattacct
    79021 ggtacataag ggaatttcaa tgttcgtatc tctttcattt ctcctaaacg caaggcaaca
    79081 tcataaggat tcacactgaa tgcttttatt tctacacgaa catgtccaga accgacttct
    79141 ctgggatgag catcgatttc ttcaaaaaca tcttgtgctt ctccgtaacc gttaattgca
    79201 aatcttcgca tggtcaccat ctcccttttt ctttaagttt acgtttatgc ctttattttg
    79261 acaaaaaaaa tgtcttttcg ttttggcgca tgattcaaaa caaaatagtt gtcttttttc
    79321 gcgtaacgtg ttaaattatt tatctaaaag cgtaaagatt acgatttacg ctaataaaaa
    79381 ataattggag gaatttgttg atggctaaaa aatcaaagat tgccaaagca aaaaaacagc
    79441 aagaaacgat tgaacgctat gctgctttac gccaggaatt aaaagcgcaa aaagattacg
    79501 aaggcttgcg taaacttcct ttagatgctc gtccagaacg attgaaaaat cgtgatttaa
    79561 tcgatggacg ccctcgcgga tacatgcgta aattcgggat gtcccgtata aaattccgtg
    79621 aattagcaca taaaggtttg attccaggag taaaaaaagc cagctggtaa tccaagtctg
    79681 gctgtaatgt aaaggaggag ggcgcatgga ccactccttg gatcatcaaa aaagatacgt
    79741 tcgcttcttc tagcagaaat gctggtacta gaagcagacg tatctttttt tacgcacagc
    79801 tggtcctttt ttatatgtaa aggctgcatg gttacttcgc tatatttatt tagtaacagg
    79861 agtcagtgct tcgataccca cactaatttt caatgctcgg tcaacttctg ccataatggg
    79921 tcgatccaaa tggcagacct tttctttcaa tcttgattta tcgatcgttc tgatctgctc
    79981 caacaggata acggagtctc tttctattcc tgttccagta gctttgatcc caatgtgagt
    80041 cggaagctta ggtttagcca tttttgccgt tatggccgca acaatgatcg tcgggctaaa
    80101 atggttacct agattatttt gaataaccaa aactgggcgt gttccgcctt gctccgatcc
    80161 cacgactgga gaaagatcag caaaataaat atctcctctt tttaccatag gatcctcctt
    80221 attcttgata ttctcttggt atccgctgtc ctaatccgca agccacttca tagtggatcg
    80281 tacctaattg atctgctata tcctgcatag tgatttcttt gtctgcattt ttgccgataa
    80341 gcgtcacttt tgttccgaca ggaaattctt gaggcaatcg gatcatcagt tgatccatac
    80401 atacacgccc aatcgtttca cagtagttcc cttctaccaa tagagaaaaa ccctgcatct
    80461 ttcgtggcca gccatctgca taaccgatcg gtatcgttcc gatccactct gcttcaggag
    80521 tgatatatgt ttctccatag ccgattcctt cacctgctgg gagttttttg acttgaatca
    80581 attcagaaac cagctcaagt gctggctgca atggataaac ttccggtagt gcgtgcccag
    80641 agggattaag tccgtacatt gctactccat aacgaatcat attgccgatt gtttcatcat
    80701 gccataaagc cgttgcacta ttgctgacat gtacatatcg aggaagttcg ggaagttttt
    80761 tcagcacttc tataaagcgt tctttttgca gattccaata tgtatcatct gcctgatctg
    80821 ctgtagaaaa atgggtaaat aaaccttccc attctaatac acgactttct ttgatgaatg
    80881 cagctgcctc ttttacttct tcaggtgtac aaaatccgat acgacccatc ccagtgtctg
    80941 cttttagatg aatcgaaaga ggcgtctcta gcttactttt ctctaaaaca gcagctgctg
    81001 cttgcaacca ttcccgtgta cctgcagtta ctgataaatc atgagcaaca gctacagtca
    81061 gtgcgtcgta aggaaccata ttcaagataa ggatgggctg agtaaatcca gcttctctca
    81121 actcgactcc ttcatctaaa tttgaaacac aaaagcccga agctccacct gcaactgctg
    81181 cctcagctgt ttcaatagct ccatgaccgt atccgttcgc ttttactact gcaaacaatt
    81241 cttttccttg cggaagtctt gcaacttcat ttgctacatt ttcagttata gcttttttat
    81301 gtatcactgc tttcgttggg cggtgccaag cgacaaccat cgtttttcct tctttctagt
    81361 cttccaaaat aatttgtgca aaagctgttt catttgtatg agtaatactg acccatacat
    81421 tgccttcatg aggtgaacgc gtcacaacag gctgaccatt ttcatttttt aaaatctcga
    81481 catcttgaaa ggtaactttt ccgatcccag ttccccaagc tttagagaaa gcctctttgc
    81541 aagcgtaacg cccgcctaaa aattctactt ggcgatgaaa aggcaaggac tggaatagtt
    81601 tcatttcatc agaggtcaag attcgtgcaa tgaattttgg tttttcttcg atcagccgag
    81661 tgatcctagg aagttcgact gcatcgatcc caattccttt tatcatatgc ttttctcttt
    81721 tcaataaatt tagaacactc tcattctacc aaattataac cattttgcca caaataagtc
    81781 cacaaataac aaaaatgaaa gtttctctta aaaaagagaa actttcattt tatgtttaat
    81841 cgttatttga acggattaca aatccgcgtt ttttatcatt gtgacgttta ccgccatctt
    81901 tttggctgtt attatttttg ctataaccgc cttttgattt gtttccacga taattaccgc
    81961 cgtcacgacg attacggttg tttccgccac cacgtttgcc atttttattg tatccttttt
    82021 tctgcattgg caatgggcgt tctggtgtga tcttcactgg cactgcatca gctggatctt
    82081 ttgctgtcgt ttttagcaaa agtgcaacta aatcttgtgc agaatattct tctaataatt
    82141 tatctgctgt ttgcaggtat ttatctagac cattttctgc aagttttgtt tcgatttgtt
    82201 caactgcagc acctaattga cctttgaatg cttctttttc ggttggtgga cgtaaagtcg
    82261 tcatccgttt tttcgtcaag ttttcaatca catgcaagta gtccatttca tttggtgtaa
    82321 cgaaagtcac agacataccg ccttttccgg cacggcctgt acgtccgata cggtgaacgt
    82381 agctttctgg atcttgaggg atatcatagt tgtacacatg agtgacacct gaaatatcta
    82441 atccacgtgc agcaacgtct gtcgctacaa ggatatctaa atgaccgctc ttaaatgaac
    82501 gcaatacgct cattcgtttt tgttgagaca aatcgccatg gattccttct gcacggtaac
    82561 cgcgtgcttc taatccgcgt gccaattcgt ctacacggcg ttttgtacgt ccgaagacga
    82621 ttgttaattc aggtgtttga acatcaaaca agcgagtcat gatatcgaat ttttcgtatt
    82681 cttttgcgcg gacatagtat tgatcgatca agtcagctgt catttctttc gctttgatct
    82741 tcacatgatg tggatttttc atgaatttaa cgccaatatt cttgatagct ggcggcatcg
    82801 ttgctgagaa aagtaatgtt tgtctttgat caggaacttg tgaaatgatt ttttcgatat
    82861 cttccaagaa gcccatattc aacatttcat ctgcttcatc caaaactaat gtttgaacgg
    82921 ttccaagttt caatgtgtgg cggttaatgt ggtcaagcat acgtcctggt gtacccacaa
    82981 cgatatgtgg acgatctttc aagccacgga tctgacggcc aatatctgct ccgccgtaaa
    83041 cggcttgtac acgtactttt ttgtcgcgcc ctaaacgata taattcttct tgtgtttgga
    83101 tagctaattc acgtgttggt gcgataacta aaccttgtaa ttcgtgacga tctggatcga
    83161 ttttttctaa cattggtaat ccaaatgcag ctgttttccc tgtacctgtc tgtgcttgtc
    83221 caataacgtc ttttccagct aatgctagtg gaatcgtttc tgcttggata ggcgttgctt
    83281 cttcaaaacc agcgcgttcg actgatttca acaattctgg tgataattct agttctttaa
    83341 atttcaaatg aatcctccta tatggttgtt aactgaggat gtagcttttt tgctaccccg
    83401 tcgatttgtt cggtcaaagt gtattgtttc tttttgacag tggccacgca cgtttttatc
    83461 cattcttagc ccctgtggca ccaccgaaaa atcactttgc gaagtttagg cattagaaaa
    83521 gccaaacggg tcgcgttcag ctttatcatt attatgacat ctggcttcaa caagttgttt
    83581 ctatggcaaa accagtccaa tttctcttca tctttcggca ataggttatt ctaacatagt
    83641 ttttcgtttt ctgcaagttt acagattttt atttcatttg ctgaagttgt tctaccactt
    83701 caaataagcc catgctgttg ctgcctttta gcataatact atctgttgct tcaagatttt
    83761 gtttgatagc atcaatcaat ttattctttt ctttttcaaa atagaacaca ggtagttggg
    83821 ggtatttttg gttcaattct tcttttagtg catgcatttg ttccccataa aggaaaatca
    83881 tggcgaattt ctcaggatcg atatgttcac tcatttgcgc atgcatattt aaagactctt
    83941 tccctaattc cagcatatct gctaacacag cgattctgcg tccttttgtt ggcagatttt
    84001 taaaagtatc taataccagt cccatggctg ttggatttgc attgtagaca tcgctcaaaa
    84061 tagctgcacc atttcctgcc gttagccatt cggttctgtt ttgagtgata gaaacatggg
    84121 cgagcccttg gcgtatctca ggtatggaca gtccaaagaa gcgcccgatc gtatacgcaa
    84181 tcaaagcatt cgttacatta taacttccag gcagcgggat cgtaaatgtt tctccttcta
    84241 ctacaaaatc ggtttgtgct tttccttttg ctgtcacttc cgcatgaaca tcgccatttc
    84301 caataccgaa tgttgtgact gtttgtacta atgttgccgt taaaggtgtc agcaacggtt
    84361 catctgcggg cacaacgagt aatccgtctg atacaagtcc gtcagtgatt tccattttgg
    84421 ctttagcgat tccttctcgc gacccaagat tttctatgtg tgcttcaccg atcattgtga
    84481 ttgctgcagc ttttggttga gccataagtg aaagttccgt gatttcattg gcgtgatcca
    84541 tgcccatttc caaaacgatc ttttctgtat cttggggcat gtgcaaaatc gtgtaaggaa
    84601 gaccaatatt attgttgtaa tttccttgcg tcttatatgt tttgtatttt tcagacaaga
    84661 cagacgcggt tagatctttc gtggtggttt taccattgct tcctgtaatt gcaacgactt
    84721 ctgcgcctac tttttccaag taataggttg ccaattgctg catggctttc aaaggatctg
    84781 ctacaggaat aactggaaaa ttttttggag cttgttcaac aggcaagctc caaaatgaag
    84841 cagatgctcc gtttgtgatc gcttgctgga taaattcatg tccatctcgc gtaccagcaa
    84901 gaggaacgaa taaacttcct tcggttatct tccggctgtc aaattcgaca ttggtaatct
    84961 ttgtcagtgc agagcattcg ctgcccacgg cttgtgcaat ttcttgtatt gttagattca
    85021 ttgagatctc tcctaaaaaa ggttgtgaaa aaagcgtttg gcctaaacgg cgggttcgca
    85081 tgactctcac ttccaagtaa atttctctat tcgtatttag aagctagaat gaacgcggtt
    85141 acgcaatcac tccagcctct tctttatttc tctttattca tttttttcaa aaaaagattg
    85201 acgctgatgg tatcgattca ttcctaactg gatcagttct tcaatcaaat caccgtattt
    85261 caagcccata ttttcccata agagtgggta catactgaac tccgtaaatc ctggcataga
    85321 gtttaattca ttcaggaata attcattttt atttgtcaaa aagaaatcgc accggctcaa
    85381 tccgcttcca cctaacatcg tgtaagctaa cttcgcgtac tcttgcgctt tttgataaac
    85441 ttcttctggc acttcggctg gaatctgcat ttcgatttta ttattgatat attttgcttc
    85501 ataatcatag aatgctacgt cttttacgac ttcaccaggc aaagtcgtcc gaacgtcttc
    85561 atttcctaat acagcaactt cgatttcgcg cgcttcaatt ccttgttcaa cgattgctcg
    85621 agaatcatac tgataggctg ttgctaaagc attttgcagc tcttctcggt tttctgcttt
    85681 tgtaatgccg acactagaac ccatattcgc cggtttgaca aacatcggat aaagcaaaga
    85741 accttcacat tgatcaaata cttttttagg attttctttc cattgattct taagtactgg
    85801 tacataagga acttgcggca caccagcagc ttgtaaaata tacttggtca tgattttatc
    85861 catggcacat gcactggtca atacgcctgc gccgacataa ggcatattca atgtctctaa
    85921 gaagccttgg atcgttccat cttccccgtt tggcccatgt aaaactggaa aaacgatggc
    85981 tccttcttct ttgatttcgc ccggattgat cacttttcct gtaaagcctt cctctgtctg
    86041 tccacttggg tcccatgaaa gatgcaagac atctttgctg gcaggttttt ctgttagtaa
    86101 tggacctttg acccattgtc cttcttttgt aataaatacg agttgaactt ggtaataatt
    86161 ataataaatg gcatttaaaa ctgaaaatgc ggaaagaatg gacacttcat gctctgcgct
    86221 gcgtccgcca tatagtaaag taatcttcaa aagattgtcc tcctgtattc taaatcgttc
    86281 agtgatttac tctcatattt tagcacaaaa caaaaataaa cgaataactt atttataaaa
    86341 aaacgagcca gcttctaaaa gaacgagcgt catttatttt ttgtcagcta gattatttgt
    86401 cccgtttttt ataaaaagtt atgataaaga aaaggaggga tgatgatggc gcaaacaaat
    86461 ccacgcagcc gcttggcaga aatcattcga gtgttcatca attacaatgt agtgccaaac
    86521 tttgtacagc aaaaaaatcc cgaacaggta aaaaaagcct ttgaagaact aggccccacc
    86581 ttcatcaaga taggacagat gctttctgtt cgagaagatt tgctttcttc tgccttcact
    86641 caaacattca aaacattaca agatagtgta cctagcgaca cattttcaac ggtcaaaaag
    86701 acgatcgaaa cagaactttc tctttcatta tcagatatat tcgatgattt ttcaaaatcg
    86761 ccttttgcct ctgcttctat ggggcaggct catcgtgcca agctgaagaa cggtgacgcg
    86821 gtagtcgtta agatccaaca ccctaacatc gccgaagaga tccgacttga tcttcaacta
    86881 tttgaacgag cgatcccttt gatcaaatac attcctgaaa ctagcgtagt tgatctaaaa
    86941 ggggtactcc aagaagtaaa acgttccttg atcaatgaaa tggacttctt aaaagaaagt
    87001 caaaatggcg aacaattcta tcaaaagaac aatggctgga aagagatccg cagccccaag
    87061 atttatgacg ctttttgttc gaaaaaagtg atcgtgatgg aagaaatgtc tggtaaaaac
    87121 ttgaatcatt taatgaacgc cgaaaacaaa actgaaactt ttatcactgg aattcaaaac
    87181 aagcagctga aacaagaagt tgcaaagctt ttagttgaga actttatgaa acaggtgttt
    87241 gatgatggtt ttttccatgc tgatcctcat ccagggaatc tgctattcca tgtactaaca
    87301 aaagaagaac agacacaagc tagccgaaaa actgaaacag tccatgaaaa agagttcggt
    87361 tcctttgctt ttcgagcctc cacttctgca gaagaccctg ttgcgccata taccatcaac
    87421 tacatcgatt ttggtatgat ggggcatctt tctgcaggac ttaggcaaaa actcacacaa
    87481 gccgttttag cgctgtatac gaaagatgct tatcgaatcg aaaaagcggt ccttcgactt
    87541 tgtcagcaag aaggctcgtt tgatgaaagt cggttccacc aagaactgac aagttttctt
    87601 gaacagtatt atgattctcc tattgatgaa atcaacttgc aagaagtatt cacacatgtc
    87661 gtgacgattt gtcatcaaaa taatctgcag ttcgatcgtg atatcacgct tttgctgaaa
    87721 gcatttggga cattagaagg ggtgatccgc gtcctcgatc ctgaagtttc cctcatggaa
    87781 gtcgcttcgc cttttgctca acattatttt ctcactcatc tggatgtaga agatacattg
    87841 aaacaaagcg gattagactt attggaaggg atgaaggctg cgcctaagat ccctcaacag
    87901 ctccaccatc tattagaaat gtggacttcg ggacaaggaa aagtcaattt ggaattgaaa
    87961 aagcaagaca aattattgag caggatcgaa agtatgatca atcggcttgt tttcggtatg
    88021 attttagctg cattgatcgt agggtcctcc ctgcttgtcc aagcagcacc tgtagaaaac
    88081 gcagaggtcg tttccttatt agggatattt acgtatgcta ttgccgcatt cgtcattatt
    88141 tttttggcaa tcgatgcatt gatccaaatg tataagaaga ggaaaaagta gaaaaaggtt
    88201 gtgacagaaa gaatcagctt caagaaataa gaaggggttt tacataaatt gtttctcaaa
    88261 tttttgtgaa aattcggctt atttttcgaa gaggctactt ctgaaacacc gagaataaaa
    88321 aatgtgaaaa agctgttctg cacccagtaa taagaacaat caaaaaggga acatggaaca
    88381 catttatcct gtttccctgt tccctttttg atgtattcga gcataaataa aataccttcg
    88441 ttacctacta tcttctgtac ttggtaaacc ttagtcaatt gcctttttta acgcttgacg
    88501 aaaagacata tgatctttct ccgtaaaggg ttttggaccc tttgtcctta tgccagcttt
    88561 tcttaacttt ccactatgat aacgttgttc tagaagcgtg ccgatattcg cttcgtcaaa
    88621 ttcttttcca gagtgatgga acactcgcac acccttcggc aatacaagtg aagctaaacc
    88681 ataatcttgc gtgatcaaaa agtcattctt cttaaccata ccgacgattt tataatccgc
    88741 actatcagct ccgcggtcta cgtagacgat ttggacatga ttagcgtatt ctttggtcgt
    88801 ataatggtcc acactggtca cgataaccac gtcaagacca taggattctc ctaattttat
    88861 gacatcttct tttactggag aaccatctcc atcaatgaat agcctcattc tttttcgcct
    88921 tcttccataa atgataaaca gttactaatc cgctcagtaa aagaccaaca ttgatccatt
    88981 ttgccaagat aagcagttgt acactttcta tcggcactga ttcatggttc agcgtgatca
    89041 ataatagatg gattggctct cccatttctt ttgctaaaac aaacaaaagc gtcatcaata
    89101 aaaactggtg gatcacactc cctttatttt gagtagtata cttgacaacg atccggcgat
    89161 ccattggaat agctgtatca gtttgataat attccttatt tagccagctg cctgttacta
    89221 cgtgccagaa actaagcaga aagacaccta tcgcaaaacc atttggaatc aagaggacca
    89281 ataaagaaga actcagtcca agcaattgca tagccaatgg agaccatgtt aaacgtttca
    89341 aaatccatgg tccgactatt tttgctgcaa tcaagcctgc tgcataaggc aaatacataa
    89401 aaatcgaaag tttttctaat ccgtacagta tgccaacata aaaactgcta aataaaaaaa
    89461 ggaaattccc tagcattcca tttagaaaag taaacagatt tagccagctt ggcaacttcc
    89521 attccttctt ctggtgtcct aaaacaaaca cgaacaagat gaacagcagc aagaaaccat
    89581 aaatagcaag atcaaaaagc ctttcatcga ataacaatcg ggcatttctc aaaagaaaca
    89641 ataagccaaa gaatatcaaa aataaaacaa gctctcgcgt agctaccaga tttcttttga
    89701 tgtctttgaa atccagttcg taacgcggat aatgactaac cgtatggtag gccatgacgt
    89761 aaaaaagtgt ataaatagta aagcttgctg caatctgagt cggtgcaggc aactcaatcg
    89821 cttgatacag gagaaatagc cagatagctg caaataagta ctttttccca tccatattga
    89881 taaaaccttg cgtcttttcg tgaaaattga ccgtggtatt agcaggtaca agccatgctg
    89941 cactcagccc tagcaaaaca cttgaaaaat aaaataaagg aaaataaaat acgcctaata
    90001 ttccaagcaa tgaaccagca ccaccaatca gcagagcgat catcagcaac gtgtaactat
    90061 ctagaccaaa tcgtagactg ttgactaaaa atagtccagt catccgaaag gcataaaaca
    90121 gcgtaaatgg caaaatcgtc agccaattat gcgagtctgt tagtcccaaa aataaaagat
    90181 aaggaataaa aataaccgtg ttacttaaga taaagggcgt tgtttgtgta aaattacgcc
    90241 caaatcgttt gagtttttcg aacatgacaa tggctcctta aacagtctta taactaatct
    90301 gctcttcgat tttagcatta aaggggaaat aaaccaattt tacagaatat attcttgtaa
    90361 aaaaaaatac agaaagaagc ataagcttct ttctgcattt ttacggtact tgattactca
    90421 agcagacaag cccttctcca caaaagtctt gcccgacacc cctgcatccg gcaggtgata
    90481 aagatagaaa tcaattatcc tgtgcctcca aaatcaatta ggagctctgt aaaccagagt
    90541 ccttctcgac ccgtcgcata aatttacttt aacacagttc ctatgtttat gcgagagctt
    90601 ttttcatttt ataatcgacc atttgaaatc cttgaccgat atctgaaata agttcttctg
    90661 taatctcaaa tccttcatgc ttataaacct cgattgcttg atgattcccc tgattcactc
    90721 gcaaaaagat ctctctttcc tttgtttgcg cagcaagttc ttcataccag cgaaaaactg
    90781 aagaagtcaa tccttgacca cgtactttat ttagcaaata taatttactt aaataaattt
    90841 ttccatttat ctcttcataa gcagtgtaac cgacagtctt ttcttcgctt tttaataaaa
    90901 agtaggacac accttctgtc agttcttttt gaatctgttt ttttgactga taagttttca
    90961 gcatgtaatc cacttgtttt tgaccaatga tcggcacaaa aacttccggc catacttctt
    91021 gtatgactgc catcagctca tccagttcat tttctgttac ttttattttt tccatacacc
    91081 cacgtccttt tttacattat aaatgacctt gcgcttgatg gaataaaaag agcatgatca
    91141 tgctatacac acccaccgac cacggtttca agaaaatcac aatctgcata aattccctaa
    91201 aagataattt cgtcaaacca gctactagac agatcaagtc gtctggcgca aagggaagca
    91261 gcaaggtaac aactagtact tttttaatat ctttcgtttt tcttgtcagc aattcatcaa
    91321 atttctgata cttttttggc gataaaataa gctggacaaa ggctacgcca tagtaacgga
    91381 caagcaaaaa tccgattgct tccccaatca ctaggccaat acaactatat aaaagcccaa
    91441 atccattgcc aaacatcagc atgcccgcta ctgaggagat ccctcccggc aaaataggaa
    91501 tgatcacttg gatcacttgc aacagaacaa atatcatcac tgcatacttg ccaaattgct
    91561 ggataaacgc ttgcaaagac gtgctcgatc gaaaaatccc atgatgataa gcataaagaa
    91621 tcaacacaaa gaaaaaaaac agaccaataa tgggcatgat ctgaagacat ttttgtaaaa
    91681 ctgatccttt tttagacatc tatattcccc acatcttttt tcatttatta aaactgcctc
    91741 ctt
//
LOCUS       DACDUU010000002        84516 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.002, whole genome shotgun sequence.
ACCESSION   DACDUU010000002 DACDUU010000000
VERSION     DACDUU010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 84516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.002"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(330..2711)
                     /locus_tag="IWL30_000088"
     CDS             complement(330..2711)
                     /locus_tag="IWL30_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP9704891.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2701..3348)
                     /gene="recU"
                     /locus_tag="IWL30_000089"
     CDS             complement(2701..3348)
                     /gene="recU"
                     /locus_tag="IWL30_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAP9704892.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3412..3951
                     /locus_tag="IWL30_000090"
     CDS             3412..3951
                     /locus_tag="IWL30_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAP9704893.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4041..4460
                     /gene="gpsB"
                     /locus_tag="IWL30_000091"
     CDS             4041..4460
                     /gene="gpsB"
                     /locus_tag="IWL30_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAP9704894.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4482..4849
                     /gene="rnpB"
                     /locus_tag="IWL30_000092"
     ncRNA           4482..4849
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IWL30_000092"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5058..6224
                     /locus_tag="IWL30_000093"
     CDS             5058..6224
                     /locus_tag="IWL30_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAP9704895.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6270..7766
                     /locus_tag="IWL30_000094"
     CDS             6270..7766
                     /locus_tag="IWL30_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAP9704896.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7847..8530
                     /locus_tag="IWL30_000095"
     CDS             7847..8530
                     /locus_tag="IWL30_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9704897.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9175..10365
                     /locus_tag="IWL30_000096"
     CDS             9175..10365
                     /locus_tag="IWL30_000096"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAP9704898.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10467..11420
                     /locus_tag="IWL30_000097"
     CDS             10467..11420
                     /locus_tag="IWL30_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAP9704899.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSGNVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11489..12838
                     /locus_tag="IWL30_000098"
     CDS             11489..12838
                     /locus_tag="IWL30_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9704900.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(13021..13344)
                     /locus_tag="IWL30_000099"
     CDS             complement(13021..13344)
                     /locus_tag="IWL30_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9704901.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13473..14636
                     /locus_tag="IWL30_000100"
     CDS             13473..14636
                     /locus_tag="IWL30_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9704902.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14706..16055
                     /locus_tag="IWL30_000101"
     CDS             14706..16055
                     /locus_tag="IWL30_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP9704903.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(16100..16531)
                     /locus_tag="IWL30_000102"
     CDS             complement(16100..16531)
                     /locus_tag="IWL30_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9704904.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16919..19561
                     /gene="alaS"
                     /locus_tag="IWL30_000103"
     CDS             16919..19561
                     /gene="alaS"
                     /locus_tag="IWL30_000103"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAP9704905.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKEHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19761..20357
                     /locus_tag="IWL30_000104"
     CDS             19761..20357
                     /locus_tag="IWL30_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9704906.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20479..21627
                     /locus_tag="IWL30_000105"
     CDS             20479..21627
                     /locus_tag="IWL30_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP9704907.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21665..22078)
                     /locus_tag="IWL30_000106"
     CDS             complement(21665..22078)
                     /locus_tag="IWL30_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAP9704908.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            22157..22588
                     /locus_tag="IWL30_000107"
     CDS             22157..22588
                     /locus_tag="IWL30_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAP9704909.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22687..22920)
                     /locus_tag="IWL30_000108"
     CDS             complement(22687..22920)
                     /locus_tag="IWL30_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAP9704910.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            23103..24773
                     /locus_tag="IWL30_000109"
     CDS             23103..24773
                     /locus_tag="IWL30_000109"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAP9704911.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            24920..25306
                     /locus_tag="IWL30_000110"
     CDS             24920..25306
                     /locus_tag="IWL30_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9704912.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            25293..25991
                     /locus_tag="IWL30_000111"
     CDS             25293..25991
                     /locus_tag="IWL30_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9704913.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            25996..26460
                     /locus_tag="IWL30_000112"
     CDS             25996..26460
                     /locus_tag="IWL30_000112"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAP9704914.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26457..27368
                     /locus_tag="IWL30_000113"
     CDS             26457..27368
                     /locus_tag="IWL30_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP9704915.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27603..28142
                     /gene="pyrR"
                     /locus_tag="IWL30_000114"
     CDS             27603..28142
                     /gene="pyrR"
                     /locus_tag="IWL30_000114"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAP9704916.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            28156..29439
                     /locus_tag="IWL30_000115"
     CDS             28156..29439
                     /locus_tag="IWL30_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAP9704917.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29452..30378
                     /locus_tag="IWL30_000116"
     CDS             29452..30378
                     /locus_tag="IWL30_000116"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAP9704918.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30397..31677
                     /locus_tag="IWL30_000117"
     CDS             30397..31677
                     /locus_tag="IWL30_000117"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAP9704919.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            31682..32758
                     /gene="carA"
                     /locus_tag="IWL30_000118"
     CDS             31682..32758
                     /gene="carA"
                     /locus_tag="IWL30_000118"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAP9704920.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            32763..35948
                     /gene="carB"
                     /locus_tag="IWL30_000119"
     CDS             32763..35948
                     /gene="carB"
                     /locus_tag="IWL30_000119"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAP9704921.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            36002..36778
                     /locus_tag="IWL30_000120"
     CDS             36002..36778
                     /locus_tag="IWL30_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAP9704922.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            36775..37707
                     /locus_tag="IWL30_000121"
     CDS             36775..37707
                     /locus_tag="IWL30_000121"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAP9704923.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            37704..38420
                     /gene="pyrF"
                     /locus_tag="IWL30_000122"
     CDS             37704..38420
                     /gene="pyrF"
                     /locus_tag="IWL30_000122"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAP9704924.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38427..39056
                     /locus_tag="IWL30_000123"
     CDS             38427..39056
                     /locus_tag="IWL30_000123"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAP9704925.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            39044..39358
                     /locus_tag="IWL30_000124"
     CDS             39044..39358
                     /locus_tag="IWL30_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9704926.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            39396..40037
                     /locus_tag="IWL30_000125"
     CDS             39396..40037
                     /locus_tag="IWL30_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9704927.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            40068..40772
                     /locus_tag="IWL30_000126"
     CDS             40068..40772
                     /locus_tag="IWL30_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAP9704928.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            40769..41671
                     /locus_tag="IWL30_000127"
     CDS             40769..41671
                     /locus_tag="IWL30_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9704929.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(41829..43535)
                     /locus_tag="IWL30_000128"
     CDS             complement(41829..43535)
                     /locus_tag="IWL30_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAP9704930.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            43725..44348
                     /locus_tag="IWL30_000129"
     CDS             43725..44348
                     /locus_tag="IWL30_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9704931.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            44798..45847
                     /locus_tag="IWL30_000130"
     CDS             44798..45847
                     /locus_tag="IWL30_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9704932.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            45844..46725
                     /locus_tag="IWL30_000131"
     CDS             45844..46725
                     /locus_tag="IWL30_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9704933.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            46728..47498
                     /locus_tag="IWL30_000132"
     CDS             46728..47498
                     /locus_tag="IWL30_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9704934.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            47777..48631
                     /gene="aroE"
                     /locus_tag="IWL30_000133"
     CDS             47777..48631
                     /gene="aroE"
                     /locus_tag="IWL30_000133"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAP9704935.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            48809..49972
                     /locus_tag="IWL30_000134"
     CDS             48809..49972
                     /locus_tag="IWL30_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP9704936.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            50089..50499
                     /locus_tag="IWL30_000135"
     CDS             50089..50499
                     /locus_tag="IWL30_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP9704937.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            50673..52901
                     /gene="topB"
                     /locus_tag="IWL30_000136"
     CDS             50673..52901
                     /gene="topB"
                     /locus_tag="IWL30_000136"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9704938.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            53039..53356
                     /locus_tag="IWL30_000137"
     CDS             53039..53356
                     /locus_tag="IWL30_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9704939.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            53516..53752
                     /locus_tag="IWL30_000138"
     CDS             53516..53752
                     /locus_tag="IWL30_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9704940.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            53749..54148
                     /locus_tag="IWL30_000139"
                     /pseudo
     CDS             53749..54148
                     /locus_tag="IWL30_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
     gene            complement(54815..55759)
                     /locus_tag="IWL30_000140"
     CDS             complement(54815..55759)
                     /locus_tag="IWL30_000140"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP9704941.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(56055..56999)
                     /locus_tag="IWL30_000141"
     CDS             complement(56055..56999)
                     /locus_tag="IWL30_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9704942.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            57220..58434
                     /locus_tag="IWL30_000142"
     CDS             57220..58434
                     /locus_tag="IWL30_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9704943.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            58456..60471
                     /locus_tag="IWL30_000143"
     CDS             58456..60471
                     /locus_tag="IWL30_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAP9704944.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(60554..61927)
                     /locus_tag="IWL30_000144"
     CDS             complement(60554..61927)
                     /locus_tag="IWL30_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP9704945.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(61953..62237)
                     /locus_tag="IWL30_000145"
     CDS             complement(61953..62237)
                     /locus_tag="IWL30_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9704946.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(62239..64254)
                     /locus_tag="IWL30_000146"
     CDS             complement(62239..64254)
                     /locus_tag="IWL30_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9704947.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(64433..64738)
                     /locus_tag="IWL30_000147"
     CDS             complement(64433..64738)
                     /locus_tag="IWL30_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAP9704948.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(64827..65858)
                     /locus_tag="IWL30_000148"
     CDS             complement(64827..65858)
                     /locus_tag="IWL30_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP9704949.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            66065..66940
                     /locus_tag="IWL30_000149"
     CDS             66065..66940
                     /locus_tag="IWL30_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9704950.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(67092..67934)
                     /locus_tag="IWL30_000150"
     CDS             complement(67092..67934)
                     /locus_tag="IWL30_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9704951.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(68026..69903)
                     /locus_tag="IWL30_000151"
     CDS             complement(68026..69903)
                     /locus_tag="IWL30_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9704952.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(69900..71321)
                     /locus_tag="IWL30_000152"
     CDS             complement(69900..71321)
                     /locus_tag="IWL30_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9704953.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(71324..74713)
                     /locus_tag="IWL30_000153"
     CDS             complement(71324..74713)
                     /locus_tag="IWL30_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP9704954.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            74970..76361
                     /locus_tag="IWL30_000154"
     CDS             74970..76361
                     /locus_tag="IWL30_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9704955.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKKM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(76434..77081)
                     /locus_tag="IWL30_000155"
     CDS             complement(76434..77081)
                     /locus_tag="IWL30_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9704956.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(77089..77775)
                     /locus_tag="IWL30_000156"
     CDS             complement(77089..77775)
                     /locus_tag="IWL30_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9704957.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(77781..78854)
                     /locus_tag="IWL30_000157"
     CDS             complement(77781..78854)
                     /locus_tag="IWL30_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP9704958.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            79076..79750
                     /locus_tag="IWL30_000158"
     CDS             79076..79750
                     /locus_tag="IWL30_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9704959.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            79755..81094
                     /locus_tag="IWL30_000159"
                     /pseudo
     CDS             79755..81094
                     /locus_tag="IWL30_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
     gene            complement(81243..82229)
                     /locus_tag="IWL30_000160"
     CDS             complement(81243..82229)
                     /locus_tag="IWL30_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAP9704960.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            82953..83984
                     /locus_tag="IWL30_000161"
     CDS             82953..83984
                     /locus_tag="IWL30_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9704961.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            84093..>84516
                     /locus_tag="IWL30_000162"
     CDS             84093..>84516
                     /locus_tag="IWL30_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP9704962.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQI"
BASE COUNT        26679 a        15112 c        17152 g        25573 t
ORIGIN      
        1 gaagccttct ttcaaaaaat tattcaaaac catatattgt attttttatt tgataataac
       61 aatgggatta ttaagaagtg gcactaaata aaagactagt gtgtatttag ccgatttaat
      121 tataaacaag taaatggata aagtatatgt aatgataaaa gaaaaatgga ttttctaatt
      181 tttaagctaa atgttagatc acacatgtat atgctggata gtcttataaa aaatcatgta
      241 atacagcttg gtacatgatt tttatttgta gctgtttttt acatagacac aaaaaagaaa
      301 ccacgcttgt agacgtggtt tcttttttac tatctgtttc cagaagctga ggtgctgctg
      361 gatggtgtat ggtcatttgc agcatccgct ccgctagttg ctggctcact ggaagaagca
      421 ggaggagcag atgaagacgg tgtcgagctt tcttcggatg aagaagaagg aaccgttgtt
      481 gaagacgctg gctcgcttgt ttctgctgtc gtgctttctt ttgaggactc agcagttgat
      541 tcgctttgac tagtagagct agagctttga gtcgttgtct ctgttgttga gcttccagga
      601 gtctggacat agctggaaga tggaatagtt gttgatggtg taattgcatt cgatctagct
      661 gtatattgat ctttgtagta cagttcaccg cccacacgaa ttaatccgct tggcatctcc
      721 cagtctgtat ttgtcacgtt agctgaaaca tactgcataa gctcgcgata aacatcagag
      781 gctacgtgtg acgattctga agtgacaggt gtcatttttt tattgtaacc tgtccacacg
      841 gaaatcgaat aatttggcgt atagcccgca aataggatat ctggatacac cccagaagaa
      901 ataccaagtt ttgcatactc gtcatctgtg taattggatg ttccggtctt accggcttga
      961 tataacccag cgatttgcgc attcgtacca gttccttcag tgatcgtatc ttttaaaatg
     1021 tctgtgatca tataagctgt ttctggagac atggctgttt ttccatctgg ctcataagtt
     1081 tcttctgttc cgtcttggaa gacgatttta ttaacatatt gaggtttgta ataagtccct
     1141 ccattggcga aagccgcata ggctgctgcc atttttaagg aagaagctcc gtattttgtc
     1201 ccatcttgtt cttccgtatt actagagatc gcattcgatt gatggatcgt actgtattcg
     1261 atcccaagat tttttaagaa agaagccact ttatctgaac cgacttcatt gaataatttg
     1321 actgctggaa cattacggga taagtaaagt gcttggcgta aagtaatggt gcccatatat
     1381 tgattatccc agtttcctac aggagtcgaa gtcccttcat agttatacgg tgcgtcagta
     1441 atagtttttc cagtagaata tttcaaatat tcaaaagctg gtccgtaatc tgtgacaggt
     1501 ttcatcgtag aaccaaaatc acgggaagta ttcactgcta agttgttgcc taaagttacg
     1561 tcttctgcga tgtgacgtcc gccaatctgg gcttttactt tcccggtatt ggtgtcgata
     1621 agggtggagg caacttgcat ctcgtcgtct gggtaagaga catactgatc cgtgttgacg
     1681 atatcatata gtttcttttg ggcgtccaga tccaaattcg tataaatctc taaaccgtct
     1741 gtgtagacat tcttatctgt tttttcttgt acttcattga tgacttcttt gacataatta
     1801 tcgacgatct tagtattgtc atcggattga gttagttctt gcagaccatc cgttaccggt
     1861 acattaacag cttgatcata ttctgtttga gagatttttt cgttttgaag catcgtatac
     1921 aaaacagtat ctcgtctttt cttcgcctga tctggataaa cataaggatc ataggcggaa
     1981 ggggcttgag gcatcccagc aagcaatgct gtttgcggta aagaaagttc tgataatttc
     2041 ttaccaaagt acatctcaga agctgtctcc atcccgtaca agccgtttga catatacact
     2101 ttatttacgt agtaggtcaa gatctcttgt ttcgattttt tttgttccaa tcttacagcc
     2161 atccacgctt cttgggcctt gcgtttcaga gtctgatcct cggcactggt ggagaagaag
     2221 gaaagtttga tcaattgctg tgtcaaagta cttccgcctt gcagtccgcc agaggtaaag
     2281 ttagagaggg cagaaccgat gatccgaata gggtcaaccc cgatgtgttt ataaaaacgg
     2341 cgatcttcta ccgacacgat cgcatcttct aatgttttcg gtaattcatt cgcagaaatt
     2401 ttttcacgct tttctgcgcc taaatcttcg aatagttcgc catcttgtgt atatagtttg
     2461 gaagatacgg tagcgtctaa ttttttatcc gtcaattcgg gcgcatcttt tgcgtaatac
     2521 cagaaaagtc ctacacctgc taaaaagagg atacagaaaa atgagagtat tcctaaaagg
     2581 atcttgataa ataatccttt tttatgtgtt ccagaggact ttccagaacc tttgccagaa
     2641 ttcttcttca cactcttttt aggagtaggc tgtttctgtc gtctagagct tctagtttgt
     2701 tcatttgcca ttcttgcttt ctccttcaat agataatgtt gataaataga agtcaaccgc
     2761 gtccaaataa ggaatccgcg gagaaatacc agttttgatc tctattccta gttcttcgat
     2821 caaagataaa ggtagtgatt tgcgtccttt ttccttttgg gcgtcccagt aagcaatcaa
     2881 atgaacacta ttcaaataaa aacagcgctt caaagacgaa aaccataaca agacaaagca
     2941 gaccccattt tgttcgaggc atgctttcat atgttcgatt tgatgggcat ggaagttttt
     3001 aaagggaaaa gatgttttgt tcttggtttc ttttgcttca aagtccaagt aaaagcctcg
     3061 atagacgcca ttatagtctg tagttgaggc ttgggtaaag taggcttcct tgatcacagc
     3121 agcacttctc ttaggatagt ctactttcac gatttggata ggggtaggct ttttatggat
     3181 aaccgccaac ccgcgggaaa gataatattt attactttca ttgattgctt cttcaaagcg
     3241 catcccccga ttgccgaatt cagtgatctg atttcttttt tcttgaattt tttcgacggg
     3301 agaggcattc ttataataag gttggccatt tggatagcgc aataccatct ttaatcacac
     3361 tcctaggcca cattatagca aaaaagcgga tagaaagaaa aggaatctga gatggataag
     3421 tcaaaagtaa tctatgtgtc tgggtatcga agtttcgaac tcggtgtttt taaagaaaat
     3481 gatcctaaaa tccaagttgt taaaaatgtt ttaaaaaaag aactccatca gttagcagaa
     3541 gaagggctgg aatgggtact tgtcagtggg aatttgggcg tcgaactatg ggctgtgcaa
     3601 atagtagcgg aattgaaaga agaatatccg gaactatctc ttggtatttt gtatcctttt
     3661 tctgattttg gtaataactg gaatgaaggg aaccaagaaa aaaaacaatt agcagaacaa
     3721 ttagcagatt atgtcgaagc tgttagccat caaccctacc aatcacctag tcagctaaag
     3781 aatcacactc gttttatact tgagcataca gtgggctgtc tgctgatcta tgatgaagaa
     3841 ttccctggaa aaacgaagta tttttttgaa gatgcacaga attaccaagc agatcatccc
     3901 tacgaaattc ggctgatcag tatggacgat cttcaaaatt ttagtgaata atcgatattg
     3961 tgtttgattt atcaactttt ttcctataca atgaaaaagg tagaaatgtt attacgtaaa
     4021 tgaaaactag aggtgtaaat atggcgaatt tggtttatag tcctaaagac attttacaac
     4081 aagaattcaa aacaaaaatg cgcggctatg atcccgttga agttgatgaa ttcttagaca
     4141 atatcatcaa agattatgaa acttacagca aagaactcct tgctttacaa gaagaaaacg
     4201 atcgattgag tgcaaaagta gctcaattat ctaaaactca aggagcagct caaacacgcg
     4261 tgcaacaaac agaggcacca aagagcgcag cagtgaccaa ttttgatatc ttaaaacgct
     4321 tgtcaaatct tgaacgtgaa gtatttggta aaaagcttga tcaacaagca agtgcagtca
     4381 aaccagcaca acctaatcca aataactaca caaatgcaga cacaagctta gatgataatg
     4441 aaaaaacacg tcaattttaa tttttgatta atgaatttgc ggttttcggg taatcgcggt
     4501 ctgacttgat caggctgagg aaagtccatg ctcgcacagg ctgtgatgcc tgtagtgttc
     4561 gtgcttagcg aaaccataag ctaaggtact ctttggagta acggcaggaa aacaagctaa
     4621 ggcttcggct atgcttaagt atccttgaaa gtgccacagt gacgaagcgg ctggggaaac
     4681 ccatccggtg gaacgcggta aacccctcga gcgagcaacc caaacaatgg taggggcgct
     4741 ttttgtctgg aagaagaaca ggacaaaaag gcagtataac tgcagataga tggttaccgc
     4801 tttgtttttt ctccctggaa aagcaaagta cagaacatgg cttatagaaa accgcaaaca
     4861 tcagggaatt aggtcgtgac aaaagtaatg cgttataaga aacaagaaaa acatttgaaa
     4921 gctatttttc aatacttcag atgaatcgtt cttattacaa aacaaacttt tgtatcaccg
     4981 gttattcgga atttgagtaa aagtgtgggg taggactgat gtcccacctc ctttttttat
     5041 aaataaggag attgtgtatg gaaaaaaaca ggaagttcaa cctcgtcgca actgcggcaa
     5101 gcgggctaga agcactggtt ggtaaagaat taagagatct tggaatcgaa tgccaagttg
     5161 agaacggacg tgcacgtttt gaaggaacaa tggaaacgat cgctacagct aatttatggt
     5221 tacgtacagc agatcgagta aaaatcgtgg ttggggaatt cgatgcgtat acgtttgatg
     5281 aattgtttga aaacgtaaaa gcattgcctt gggaagattt tcttccacta gatgcagctt
     5341 ttcctgtagc tggaaaatcg atcaagtcga aactatatag cacacctgat tgccaagcga
     5401 tcacaaaaaa agcaatcgtt gaacgtctcc gtacctatta tcaccgtcca gcttctgtac
     5461 cactgacgga aactggcgca cattttcagt tggaagtagc tttgctgaaa gaccatgtga
     5521 tggtaactct ggatacgaca ggaccaagtt tattcaaacg tggttatcgc ttagaaaaag
     5581 gtggagcacc attgaaagaa aacatggcag cagcacttat tatgttgaca aattggcgaa
     5641 aagaccgtcc attttatgat cctgtatgtg gttcaggtac gctttgtatt gaagcagcct
     5701 taataggcca taatatcgca ccaggattca atcgtgagtt tgcttgcgaa tcttgggatt
     5761 ggttcagtca agaaatcatg gaaaatgttc gatcagcagc cgaagaaaaa gcagattatg
     5821 atattgaatt agatattact ggttcagata tcaatggacg gatgattgaa attgccaaag
     5881 ccaatgcaga agaaatcgga ttaggctctt cgattacctt taagcaacaa gcagtcaaag
     5941 acttcaaaac ggataaagaa tacggggtca tcgtggctaa tccgccttac ggagaacgtc
     6001 taggagaaga agaaactgtt cggaaattat ataaagaaat gggcgatgta ttccgtccat
     6061 taaaaacatg gagtaaatat atcttgacta gcgacttagc gtttgaggaa ttttacggac
     6121 aaaaagcaac caaaaaaaga aaattgtaca atggtgcatt gcgtacggat ctattccagt
     6181 attggggaac tcgtccgcct agaaaaaaag agaaagacga ataatcttac taattgattc
     6241 attcaattgg atgtttggag gaatagggaa tgaaagaaaa agagttttta caagaagtaa
     6301 aagaaatcaa tcttctacaa caagctttag gaatcctcga ttgggataca caaacaggta
     6361 tgcctgaaaa agctagtgca taccgaagtg aagtagacag ctatctttac agcatttatt
     6421 tttcaaaaaa aatcggtcca aaaatcaaag aagccattcg ctatttttct gaacatccgg
     6481 aagaattgtc ggaagttgga actgccgttt ttgccaaagt aaaagaagag tatgatttgg
     6541 aacatcgcgt accggaagat cttatgagaa aatatacagc tgccgtttct tcagcacacg
     6601 gacaatggca aaaagctaga gaaacacaac aattttctga ttttcaacac gcattgaatc
     6661 aaaatattga attaacaaaa cagttgatac cttactggag aaaagatgaa aagacgaatt
     6721 atgatgtttt gctgaatcaa tacgaacccg ggatgactgt cgaaatcctt gataaggtat
     6781 ttgatcagct taaagaagga attctttcga tccgccgtac gctccaagaa aaagggactg
     6841 aacctgaaac agacttttta aatcggagaa tgacgaaaaa acaacagaag agatttgtag
     6901 tgaaagtaat cgaacaactt ggttatgatt ttagtcgtgg tcgtttagat gacactgtcc
     6961 acccatttat gactggaatt aatccgaatg atgcacgaat cacgacacga tggaacgaaa
     7021 cagatttcaa aatggctgtt tttggagtga tccacgaggc tggtcatggt atgtatgaac
     7081 aagacatcga tgataaatat gcgtatacac cggtttatga aggtacatca atgggtatcc
     7141 atgaatcaca atcgttgttt aacgagatca ttattggaag tagtcgcagc ttctggaaaa
     7201 agcaatatcc tttctttcaa gaatgtgcag aaggaacatt cgatgatatt ccttttgaca
     7261 aattttatcg ttcattgaac catacaaaat caagtttggt tcgtatcgag gcagacagtt
     7321 tgacttaccc tctccatatc atcattcgtt atgaaatcga aaaaatgatt ttcaatgaag
     7381 gggtagatgt tgctgaacta cctgaaatat ggaatcaaaa atatgaagag tatctgggaa
     7441 tacgaccaac aaatgatgtg gaaggcatcc tacaagacgt tcattggtca ggtgcaagct
     7501 ttggttattt tccctcttat gcattagggt acatgtatgc ggcacaatta cttcattctt
     7561 tgcagcaaga aatagatgtg gaaaatgttc tagcgtcaga cgattattct ccaatcaaaa
     7621 actggatgtc agagcatatc catcaatatg gtgcatctag aaaaccaaat caattaatca
     7681 tggatgcaac acatgaaccg cttaatccgc aatacctgat cgactatatg aaagacctgt
     7741 attttaatgt atatcaggta aaataaaata caatttacaa agatagctga aattcatcta
     7801 atctatagat gttttcagct atttacttaa gaaagaagtg gaacgaatgg ataaattaca
     7861 agtgatcgct gattatagta tggaccaatt agcgcatgat caaacaggac atgggagcga
     7921 tcataccaaa agagttgtca agttagctga aaaaattttg gagacggaac cgcaggcaga
     7981 ccgctttgtg acgcttgcag cagcttacct acatgatacg attgatgata aagtcgttaa
     8041 aaatgagaac gaggcaaagc aacagcttcg tgtattcctt cgctctttgc caatagtaga
     8101 ggaacagatc tcgatgattt tttccattat cgaaaatatg tccttttcta aaaatctctc
     8161 tgaagcggcc gaactatctc tggaaggtaa gatcgttcaa gacgctgatc gtatcgaagc
     8221 attaggagca atcggcattt tgcgtactgc ttatttcggc ggtggacatg ggcacccgat
     8281 ttttgatcct gaactgtatc cgcaaacatt caaggacaaa gaaaattacc gaaaaggcac
     8341 gacagtgatc aatcattttt atgaaaaact gttcttgctg tctgataaaa tgaatactga
     8401 ttacggatat gaagaagcca aacgaagaga aaccttcatg cgtgaattct tagaagaatt
     8461 ttttctggaa tggtctattg acgatgaaaa ttttactcca gacaattgga tcaaaaataa
     8521 caaaaaatga tggaggttgt gacagaagtg gtcagctcca agatatttcg gatcattttc
     8581 cgtaaaagtt acttctggaa cactgtttac caagaggtcg tgagcctgcc gttcagctcc
     8641 gagtcacaag gaacaatttc aaaaaatagc ttctcgtgtt tgatgaatat tgtaacttgt
     8701 gattcggaat tggcaggaga acaccgttta ttcggaagaa taggactgtg acaagacttt
     8761 tgtcacagtc cttttttcga attcaacaag taaaactttt aagaaatatt cagagagtaa
     8821 aattaaaaat tgctgataaa cgaagatgga aattttatgt atttttattg gtctatgcaa
     8881 taaataaatt aaatattcag aatacaattg attttaacga aagggcttct atcaatggtt
     8941 gtgttgtatt tatctgccca ggtagagtaa aatagatcat tatctaaaag gtaattgttc
     9001 atactatata agtcatttga aagtaaaaag atactgtaaa taaaaaatgt ggttaaataa
     9061 ttctcataat cagtgtaagc gttatcaaaa atatagcata cttgctttgt agatgaatga
     9121 cacgatatat gtttggtatg ttctgcaaga cagatgaata tggaggtgga gacaatgtct
     9181 tccaaagaac taacaatgta tttagagaaa gaattggatc aactgaaaga aaaggggtta
     9241 tacaatacga tcgacatact agaaagtgaa aatggtgctt gtatcatcgt agacggaaaa
     9301 aagatgatca atctagcatc caacaattat cttggatttg ccaatcgcga agaattgaaa
     9361 aaagcatgta ttgaagcaac cgaaacttat ggtgtaggag caggagcagt ccgaacgatc
     9421 aatggttcgc tgaaaatcca tcagcaatta gaagaaaaaa tcgcagaatt caaagggaca
     9481 gaagcagcaa tcgcctttca atctggattc aactgcaata tgggcgctat ctctgcagta
     9541 atgacaaaag aagatgctat cttatcggat gaattaaacc atgcatctat tattgacggt
     9601 tgccgcttat ccggagcaaa aatcatccga attaaacatc aagatatgaa agaccttgag
     9661 aaaaaagcaa aggaagcgat agaaagtaag aaatataaaa aaatcatgta tatcacagat
     9721 ggtgttttct ccatggatgg agacattgca cgattgccgg aaattattcc gattgttgaa
     9781 aaatatggac tgatcaccta cgtcgatgat gcgcacggct caggcgtaac tggaaaaggt
     9841 gcaggaaccg tcaagcattt tggattaagc gacaaaatcg atatgcaaat gggcacgtta
     9901 tcgaaagcta taggtgttgt tgggggttat gtagctggat cgaaaacact aatcgactgg
     9961 ttgaaagcta gaagtcggcc attcctattt tctacttcgc taactccagg tgcagctgca
    10021 gctgcattgg cttcgattac tttgatgcaa gaacatccgg aacttgtcga aaaagtttgg
    10081 gaaaatgcta attattttaa agaagaattg aaaaaagtag gctataacat cggtatgtct
    10141 gaaacaccta ttactccagt cattttggga gacgaaaaag tgactcagac tttctctaag
    10201 aagctgatcg aacatgggat ttatgctaaa ccaatcgttt atccaactgt gcctcttggg
    10261 acaggcagga tcagaaatat gccgactgcg gaacatacga gggaaatgct tgatgaagca
    10321 gtagcagttt atcaaaaaat aggaaaagaa atggaaatta tttaactaaa tttagttttt
    10381 tcgtctattt aaaattagag aacatgttca cgcatatatt gttaatcatc tgaagatagt
    10441 agtaaaaaag atcagggagg atcatcatga aaaaagtatt ggtgacagga tgtttaggac
    10501 aaattggttc agagttgatc acacggctac gtcaagaact aggcagtgga aatgtcattg
    10561 cgacagatat acgcatgcct gaagtcattg agaaaaacga tatctttgag atcctagatg
    10621 taatggatta cgaaaaaatg cgcagcatag cagaacaata tcaagtggac acattgattc
    10681 atttggctgc tcttttgtca gctgttgcag aagcaaaacc acaattagcc tggaatttga
    10741 acgtgactgg gctggtcaat gcattggaag tcgctagaga attccagctg aaatttttta
    10801 cgccaagttc aatcggcgcg tttggtccaa atactccaaa agatcatacc ccacaagata
    10861 ctcttcagcg tcctacaacc atgtatgggg taacgaaggt agctggagaa ctactatgtg
    10921 attattatta tgtgaaatat ggcgtagata ctcgtggtgt ccgtttccct ggattgattt
    10981 cttatgcaac cttgccagga ggcggtacga cagactacgc agtcgatatt tattattcag
    11041 caatcaaaga aggaaagtac actagtttca ttgatcaagg tacatcaatg gatatgatgt
    11101 atatgccaga tgcgatcgat gccattataa aactcatgaa tgcagatcct gatcgattgg
    11161 ttcatcgaaa tgccttcaat atatcggcga tgtcttttga cccggaagag atcaaagcat
    11221 ctattcaaaa aatcatacca gattttgaaa tggattatga tgtggatcct gtacgtcagt
    11281 caatagcaga atcttggcca aattcactag atgtttcttg tgcgcaagag gaatggggct
    11341 tttcgccgaa atacgactta gatgtgatga caaaggatat gttggaaaaa ctgaaagaaa
    11401 aattggtaga gacggtataa aacaagaacc acatccaaaa agttttttat acttttggga
    11461 ttggctatgg tatgaaaggg gacaagggat ggaagaatat caagccacgc cagtaaaaga
    11521 agtaaaaata gaaaatgaat taaaacgtac gatgggattt tttactgctt tatcgactgt
    11581 tatgggcaca gtgatcggtg caggggtatt ttttaaagcc gcaagcgtag cagaagttac
    11641 aggatccgcc agtttgcata tgttttcttg gtttctcggt ggtatgattt ctgtctgtgc
    11701 aggactaaca ggagctgaac tggcagctgc tattccagaa acaggaggga tgattaaata
    11761 tatcgaacgg atttacggaa atacagcagc ttttttattg ggctgggcgc aagtcgtgat
    11821 ttattttcca gcaaatgtag cagccttgtc gatcattttc ggtacacagt tcgtcaatct
    11881 atttggtctt tcccagtcaa tgattgttcc cgtagctgtt acagctgctg tatcgatcct
    11941 gttgatcaat tttttaggtt ctaaagctgg aggggctttt cagtcgatta cgcttgtctg
    12001 caagcttatt cctttgtttg tgatcgtcat tttcggtttg ttccgacaag aaggagtaga
    12061 cttccaactg tttccgatcc aagcaggaga aaatctttca tttttctcag ccttgggagc
    12121 gggcttgcta gcaacgatgt tcgcatacga cggttggata cacgtaggaa atatttcggg
    12181 agaattgaaa aagccggcga aagatttgcc aaaagcaatt tctttaggaa tcatcggaat
    12241 catgatcgtt tatttattag taaatgcggt gttcttaaga accgcatcta tcgacggagt
    12301 cgcaggaaac agcaatgcgg caagtgacgt tgcaaaaatg atttttggcg gcttcggtgg
    12361 aagactggtc acagttggta tcttgatttc tgtttatgga accatcaatg gctataccct
    12421 tactggaatg cgtctccctt atgttatggc aaaagaaaac aacctgcctt ttagcaagct
    12481 ctttgctaaa cttcatgata aaactaaagt tcctgtagcc gcaggtatct tagagctagt
    12541 catcgctatt ggcatgatga tgatcggtgg ttttgataca ctaacggata tgctgatctt
    12601 tgtcatttgg attttttata cgatggtctt tgttggtgtc attcttcttc gtaaaaaaga
    12661 acctgatttg tttcgaccat acaaagttcc aatgtatccg tttattccac tagtagcaat
    12721 cattggaggg acttttatcg tatcaagcac gcttatcact cagacctttt tagcttcgat
    12781 ggggatagta ttgacacttg ctggtatccc gatttatctc tatttaaaac gtagatagct
    12841 aaaacattcg aattttatta gaactgcact tctctgccaa acaaaatatt ttatttaaat
    12901 gaggagtacg tggaagaaat gtcaaaagta ctcgttaatt ttgatcacgt aaatgtcgtt
    12961 ttgtctacga tgaaatagca aagtaaaaga cagaaatcgg gctcaaccga tttctgtctt
    13021 ttactttgat ttgcgtaact gaactattcg gcgtacgccg cgataaatat ttaataaaac
    13081 caagcaacca gaaatgaaaa tgggaagcca aatcagatag acagggtagc caagcacttt
    13141 agacaaagca tgtggagtca ttttatccat ttcaagaagt gcttgctgcc aagtctgaag
    13201 attgaagact agcatgaaac ccgaaataat caggagaata aatcctaaat aatgaattgt
    13261 agttcttttt cttacataag cgatcgtagc ataaaggaac agtaaaaaaa taggtacgcc
    13321 aaaaacaaag tacatattca gcatctcgac accccctttg tgtttatctt actttacttt
    13381 gaaagaaatg agaaataata gcctcgttac ttgcttgaaa cagcaaagga ctgctaagat
    13441 aagagagata caaagaatca aggaggagtc atatggcaag ttatatcgaa ccgattcgtt
    13501 ttgcgctgat tgtttttcca tttttagcct tagcgatctc atctgttttt tttatttatg
    13561 aatatcgaaa gtatggaaca tttctagtaa caagggcagt tattttgtat tctttcgttt
    13621 tttatttatt gtgtgcgtat tttttggtga tcttaccact tccggcaaag gagacggttg
    13681 cccaaatgac agggccaaag atggagctgc atttatttgc ttcatggcaa catttcagag
    13741 acaacacggt attggtcttg tctgaaccta gtacctacct gccggcaatg aagcagagtg
    13801 tatttttgga accagctttt aatgttttat tactcatacc atttggcatt tatttaagat
    13861 attatttccg gctttctttt atgaaaacga tgattgctag tttctgcctt tctctatttt
    13921 ttgaattgac gcagctaagt ggactttatt ttatctaccc aagaccatac cgcttatttg
    13981 atgtgaatga tttagtgaca aatacattgg gtggaatcat tggttttgtc gttacgccgt
    14041 tatttacatt tatgcttcct acaagaagac gaatggatga actttcttat gaaaaaggaa
    14101 agactgtctc attgactcga cgcttggtcg cttatttgat cgactggaca tttttatctc
    14161 ttattaattt ggtagcggcg attttcttta aaattgcgtt cgattcaggg gcagttactt
    14221 ctcatatctg gtggctttta ttagaagtag gggtatactt tatgattgta ccgtttataa
    14281 ccaatggatg gacacttggc aaaaaaattg tgcgtatccg tttggtggaa gaagaccgcg
    14341 aacgaatttc tttaaaagca ttgatgatcc gctacgggta tctgtacttt cttttttatg
    14401 gtttaagctg gttggggcag ttgcttcagc aaatcatgaa ttcgacaacg aatacggtgt
    14461 tgcagatgag tgcagtattc atttctctcg gtgtcttgtt actacaaggt atttttattt
    14521 taaatatctt gctatcattt ttgagaaaaa aacggattct gttttatgaa aaagctagtc
    14581 acacatatac tgttagcacg attggccaaa caaaggatga tgattctcga aattagctga
    14641 gcaaaaacgc tttgtctgtg ttataatgcc aaagtcgtga taatttaaag agaagaggtg
    14701 cacgaatgaa taattttgat atattccgct tcaaaccgtt tatcaatgaa gcgttaaccg
    14761 caaagggctt caaacagccc acagaggtcc aggaacgttt gattccttta attgcaaaag
    14821 gaaaaaatat cattgggcaa tctcaaactg gtacaggaaa aacacatacg tttttgttgc
    14881 ctttgtttga aaaaatcaat gcagaaaaag aggaagtaca agttgttatt acagctccaa
    14941 gtagggaact agctgcacag atctatgaag cagctacaca aatcgctaaa ttttctacac
    15001 cggagatccg tgtctcgaat tttgtcggcg gtacagacaa acagcgtcaa ttaaatcgtc
    15061 tgcatcacca acagccacag gttgtagtag gcacgcctgg tagaatatta gacatgatga
    15121 atgaacaagc gctgaagatt cacaccgcat ttgcatttgt tgtcgatgaa gcggatatga
    15181 cattggatat gggtttttta aatgaagtag accaaattgc aagtcgatta ccagaaaagc
    15241 tgcagttttt agttttttct gccactattc cagaaaaact tcgtccattc ttacgaaaat
    15301 acatggaaaa tccaatcatt gaagaaatca aaccaaaagc agttatttct gaagacattg
    15361 aaaattggct gatttcaaca aaaggtaaag atgagaatca aatgatttat caactgctga
    15421 caattggtca tccttattta gcaatcgtat ttgccaatac aaaacagcat gtggatgaaa
    15481 ttgccgatta cttgaaaaac caaggcttga aagtagcaaa gattcatgga gacatcaccc
    15541 caagagagag aaaacgagtg atgcgacaag tacagaatct ggagtatcaa tatgttgtag
    15601 cgactgattt agctgctaga gggatcgata tcgaaggtgt ctctcatgtt attaatgcag
    15661 aaattccaga agatctagac ttctttatcc atcgtgtcgg acgtactgga cgtaatggct
    15721 taaaaggaac ggccattacg ttatattcgc caaatgatga gcaagccatt gatgcgatag
    15781 aaaaattggg cgtctcattt gaaccaaaag aaatcaaaaa cggggaaatc gtcaaaacat
    15841 acgatcgtaa tagacgtgcc aaacgagaaa aatcaaaaga tgtattagat cccacgctga
    15901 ttggtcttgt gaagaagaaa aagaaaaaga tcaagcctgg ttataagaaa aaaatcgatt
    15961 gggcaattgc agaaaaaaat aaaaaagaac gcaaaatcga acgcagacag caaagccgta
    16021 ctgctcgtaa aaataagaaa aattcacata aataaaaaac aagaggttgt gacaaaaggc
    16081 gtgtcacaat ctcttgtttt taagctagac tttttttcat tccgatacaa agtacttcat
    16141 cttcaataaa aggttctgtg caagtatgat acccttgttt ttcataaaaa tctacagccg
    16201 tcatttcagc tactacataa ctagtctgac aaccttctga aaccgcccgt ttttcagcag
    16261 cttcaagcaa ctgaccgcct aacccttttc ctcggtattc ttttgctacg caaaatcgtt
    16321 ccggcttgaa gcaagtaggt gttttctttt cataacgcaa agtagctact ggtctttttt
    16381 gatcaagaaa tagataatag gtttgttgcg ggatatcttt tccgtcgaat tctagttctg
    16441 caggaatccc ttgttctaat acaaaaactt gatatctcaa aaagcagcta gctgcgcgta
    16501 tccattcatc cgctccaact atttttttca tcatattcct ccttgtcatg aatagcagag
    16561 atgtattgat tcctttattc ttatcgtacc aaaaatcgtg gagtatgacc aaaaggaaat
    16621 gaaaataaaa attgagatat tgacagtgat caatagaatg cgtatactaa ataagtacga
    16681 aaaggatatg atttgttttt taagactcac agaaaattct ttcttggctg aaaaaagaat
    16741 agtcactaat caaattgctc cctttttcta ttttggtaga aataccgacg cagctcagcg
    16801 ttatcgagtt taagaggtaa tgattacaca tcattgcaat caaggtggta ccgcgagttt
    16861 tcgtccttgg ctgtgatggt gtttttttgt atttaattta tagaaagcag gtaatgacat
    16921 gaaacaatta agtagtgcac aagttcgaca aatgtttctt gatttcttcc aatctaaaga
    16981 acatacgatt gaacctagtg cgtctttgat ccccgtgaac gatccaacct tattgtggat
    17041 caactctggt gttgcaacat tgaaaaaata ttttgatggt tcagttgtgc cagaaaatcc
    17101 aagaatcaca aatgcccaaa aatctattcg aacaaatgat atcgaaaatg tcggtaaaac
    17161 agcacggcac catacgatgt ttgagatgct gggtaatttt tcgattggtg actatttcaa
    17221 aaatgaagcc atccactggg catgggaatt tttgactgat ccaaaatgga tggcattcga
    17281 tccagaaaaa ctttatgtaa cagtttatcc aaaagataca gaagcaaaac gcatctggca
    17341 tgaagaagtt ggcctgccac tagatcacat tattgatgta gaagacaact tctgggatat
    17401 cggtgcaggc ccatgtggtc cggatacgga aattttctat gaccgtggag aagcttataa
    17461 cgatttagca gaagacgatc ctgaaaacta tccaggagga gaaaatgaac gttatttaga
    17521 aatctggaac ttagtatttt cagaattcaa ccataagcct gataacacat ttgaaccact
    17581 tcctcataaa aacattgata cggggatggg attggaacgc atggtctcaa tcgttcaaga
    17641 tgcgtcaacg aactttgaga cagacttgtt tatgcctatt atccacgccg tagaaaagct
    17701 aagcaaacaa gtaaaatatg gtgaaaaccc agtaacagat atttcttaca aagtaatcgc
    17761 agatcatatt cgcgcccttt catttgctat tggcgacggt gcattgccat cgaatgaagg
    17821 gagagggtat gtcttacgcc gtttgcttcg tcgtgctgtc atgcatggga aaaaattagg
    17881 tattgaacaa gcattcttat atcgtttagt ccctgttgtt ggggaaatca tggtaagtta
    17941 ttatcctgaa gtacttcaac aaaaagaatt tattgaaaaa gttatccgta cagaagaaga
    18001 acgtttcaat gaaacaatca atgaaggttt ggaaatctta aatcaagtga tccaagaagt
    18061 aaaaaacgaa aacgggacga ctctagcagg aaaagacatc ttcaaactat acgacacata
    18121 tggcttcccg gtagaattaa cggaagaggt ggcagaagat gaaggactga aagttgatca
    18181 tgctggtttt gaaaaagaaa tggatgcaca acgcgaacgt gctcgctctg cccgcagtac
    18241 tgaaaaatca atgggggtac aatctgcttt attgacagat atcaaagtag aaagtaaatt
    18301 cattggatac acagaacaag aatcagatag tcaattattg gtcatcgtta aaggtgagtc
    18361 tcttttgaat gaagtcgcag aaggtgaagc tcaaatgatc tttgatcaaa cacctttcta
    18421 tgcagaaatg ggtgggcaag tagcggatca aggatggatc atgaatgaag taggcgaaat
    18481 cgtagcagtt gttacagatg ttcaaaaagc gcctaatggt caatttatgc acaccgtaga
    18541 agtaaaagca ccaatgagtg aaggacaaac ctatcatcta ttgatcgatc aatcgatgcg
    18601 caatcggatc atcaaaaacc atacagcaac ccatctactg cacaaggcat tgaaagaagt
    18661 acttggcgag catgcaaatc aagctggttc tttagtcgct cctggacatt tacgattcga
    18721 ctttacccac tttggtcaaa tcacttcaga tgaattagct gaaatggaaa gaatcgtcaa
    18781 cgaaaaaatc tgggaagcta tcccagttga aacaattgaa acagatatcg atacagccaa
    18841 aaacatgggt gcaatggctt tgtttggtga aaaatacgga catgacgttc gagtagtaaa
    18901 tattggcggc tggtcaatcg aactgtgcgg aggcacacat gtagcaaata cagaaaacat
    18961 cggaatcttc aaaatcgttt ctgaatcagg aattggtgca ggcgtgcgac gaatcgaggc
    19021 tgtaacaagt aaagaagcgt acgaactact tgaagaagaa gaaaaacaac taaaacaagt
    19081 agcaacaatt gttaaatcac ctcaattgaa agaagttgtt tcaaaagcgg aacaattgca
    19141 acagcaagta agagagctac aaaaggaaaa tgaacaatta gctagcaagt tagcgaatca
    19201 acaagctggt gatgtattca aagatccaga aacgatcaat ggaaaaacaa tcattgcagc
    19261 acaagtaaaa gtgaaagata tgaatcagct gcgtcaatta gctgaccaat ggaaacaaaa
    19321 aaatatttca gatattcttg ttttagcaaa cgaacaagaa ggaaaagtca gtctgctagc
    19381 ggcaatgacg aaagaatcaa atgaagctgg actaaaagct ggcgacttga ttaaagcaat
    19441 cgcaccgaaa gtaggcggtg gcggtggtgg acgtccagac atggcacaag ctggcgggaa
    19501 aaatcctgct ggtattccag atgcacttaa agctgcgaaa gaatgggttg cgcaacaata
    19561 gggcgtttac gtttgacaga aaaacaaaag ttcaaacatt gaacgaaaca tgattatggg
    19621 ggcgaagaca aacaagtctt gccctcatac ttttttcaat tggatggcgc atctaatcaa
    19681 atggtcggat tcggatggtc tttagttgac gaaagtgaca gaaattttta cgattagtat
    19741 aaataaaaag gggtgaaaaa atgaatagta gaaataagac ctttcgacta gtcttacgtg
    19801 cgatcttgtt agcaatcatc atcgtacaag cgatggttcc ttggctgggg tttattccat
    19861 taggattcat tagtttaaca attatccata tcacagtaat tattgctgca gtagtattag
    19921 gcccaaaaga tggcatggtg attggcttgt tttggggaat cgctacaatt gttcgtgcct
    19981 atgccatgcc aacaacgcca tttgatacat tagtgttcac gaatccgatt atctcggtag
    20041 ttcccagagt acttgttggc ttagtagcag gcttggtttt ccactggatt tatcaaaaaa
    20101 ataaatcaat aacgatcagc tcagcctttg caggggtact gggttcattg gtcaatacag
    20161 tcttagtgtt aggattcatg ggattatttt atacaggtgc aacggcagat gcctatggtg
    20221 tagactctgc tcttttattt aaaaccttag caggggttgc agcaatcaat gggataccag
    20281 aagcaatagg tgcgggaatc ataacaccat tactagcaaa agcgttattt gcagcaacac
    20341 cgttgaaacc agaataatga ccatctttta atagtaatag gacgcacgat agatagaaaa
    20401 aggaagagag gagaggaaaa caatcgtcct ctccatcttt ttacctgagc aacaagcaaa
    20461 ctagaagggg taaaaaactt ggctaagaaa caagaagaaa aaaagaaaca aataccgcaa
    20521 cagcggacaa aaaaaggagt actagcgggt atcttaggat tggttacgaa tctgatcttg
    20581 tttgtctcta agctaatgat cgggcttttt tcaggaagtg tctccattat ggctgatgca
    20641 gtgaacagtc tatcagatac tgcttcatcc atattgactt tgatcggatt taaaattgct
    20701 gcaaagccag cagatcagga acatccttat ggacacgaac gcttcgaata tatcagtggg
    20761 ttatttgttt cgatcatcat tacttatgta ggatttcaat ttttagagtc ttctgctgaa
    20821 aaaatcatcc atccagaaaa tgtctcttta acacccatcg tcttttttgt cctaatcatt
    20881 tccatctttt tgaaatttgc acaaggcaaa atgtatcagg ttattgcgca atccattgat
    20941 tctgaaacat tgagagctac agcaacagat agttataatg atgtctttac tacactggct
    21001 gtattgcttt cagcagcaat cgaatgggta actggatggc gtattgatgg atatataggt
    21061 tttgctttag cattatatat cctttatagt ggattgacga tgcttcgaaa ttttgtatac
    21121 gaactattag gaagtcgccc aacgccagca gagattgaag aaatggaaga gcatttaaat
    21181 gcttgcgatg atatacttgg ctatcatgat ttattggtgc ataactatgg acccaataaa
    21241 cgctttgcat cagtccacat cgaagtagat gaatcaatga atttgaatgt ggctcataag
    21301 attattgatc tgatagaaaa agactttaaa aagtctttgg atgtggaact tgtctgtcac
    21361 ttggatccaa tgcctgttga taacgaaaaa tactataata tcttgcggca gttaaagcaa
    21421 attcttcttg aaacaggtac agggcttaat attcatgatt ttagagtaat tcatcaagga
    21481 aaaacgctgc aatttgatgt agtggtccct gagaaattcc agtttgttga tacagaacta
    21541 gaaagaatct tacgtagtca aatagaagaa aaaatcggag attaccaatt ggaaattacg
    21601 tttgatcaca attatctttt atattaaatg agaaactata aaaagactcc ttaaagtttt
    21661 taatttaaag agtctttttt tcttttaatt gtttatggag agcctgacgt gcaagattat
    21721 ctgctccttt atttttgttt tcgggcaacc attgcaggat caataaagaa aattcggata
    21781 gaagttcttg gattctttga aggtagggaa ggaagagctc gttcttcgtt gcgtttttgt
    21841 cgaccgtaga aactagtgtt ttgctgtctg aaaaacagaa aatcgtttca ttctgtaagt
    21901 cgtttttctt tagaaattct aaagttttta aaaagacttc gaactctgct tgatggtttg
    21961 tccctataga aagcgggata gatagctgtt cttgtttacc ttgatagaca atcaagattc
    22021 cacctccact aggaccaggg ttgccttttg tagaggcatc aatgtatact tttaacataa
    22081 aaagagattc tcctttttgt tcaaactaat gttatggtaa gattatatca ttactgaatc
    22141 ggtgagaggt tagagaatga aaaaaaatat tcgatggcag cctgaactgg cacatacgat
    22201 catctattgg tcattgacct tgatcattct gttttttagt ctgattcttt ctttagaaaa
    22261 cacaggtcct tattggaaaa gcaattttgt tttaggtatt tttcttgttt ttctatttct
    22321 aggtttgcgg cgttctttta ttttaaaatc aaactctgtg attatacgtt atgctgcttt
    22381 ttggaaagac cgagaaatat ctgtcggaca gattcgatct ttgacgccaa ctaatactgg
    22441 ggtgcaaatg atgttgcaag atgagaaagc cccatctgtt ttcatgatgc gaaaaaaaga
    22501 tcaagtagtc ctttttgatc attttgaaaa aaacggaccc aaatcagaat caattcctta
    22561 tccttatacg aaaaaaagaa aaagctaatt ttaaaaatca gaacctaaaa aaacgaattt
    22621 ttacaggaaa agaaaagagt gagacgtacg gttcatcccg tttagcgtct cactctattt
    22681 gtttatctat tcatgcatac tagtatcggt agtctgaacg ttgcctttct tgacatgggc
    22741 agcttgtgtt cctcgattcc cttcgatgat ctcaaattcg accacttgat tttcttccaa
    22801 agttttgaat ccctcttctt caatcgcagt aaaatgtaca aaaatttctt ctgtgtcatc
    22861 ataactgata aagccgtatc cttttttgtt gtcaaaccat tttacaattc ctgtggtcaa
    22921 agcactcacg acctttctaa agtctgatca tagccttcat tataaagagt atttataagt
    22981 acgtcaaata aattaaaaac ctgtaatttt atgttttttt tctgaataaa aaaaggtata
    23041 attggcatat cttaacaaag aagatatttt gttgaataaa gttcgtgaaa aggggtaatc
    23101 aaatgaagaa tgatctgcaa atttcacaag aaacagagct agaaccgatt gttgaagtgg
    23161 ctagtaaatt agatcttaag gaagacgatt tggaactgta tgggaaatat aaggcgaaaa
    23221 tcaatttttc tggaatcaat cgatcaaagg ataacgctga ggggcatttg atcttagtta
    23281 cttccatcaa tccaacacca gctggcgaag gaaagtcaac gatcactgta gggttaggag
    23341 acgcgctcaa taaaatcaat aaaaaatctg ttattgcatt gcgagaaccc tcacttggtc
    23401 ctgtgatggg aatcaaaggt ggtgctgcag gcggaggata tgcacaggta ttgcctatgg
    23461 aagatatcaa cttgcatttt actggtgaca tgcatgctat cacaacagct aataatgcat
    23521 tatctgctct tttagataat catattcatc aaggaaatga attgaacatc gatgcccgcc
    23581 gagtgatttg gaaacgggta gtcgatctca atgatcgtga actgcgtaaa gtaattgttg
    23641 gcttgggtgg gccaatccaa ggtgttccaa gagaagatgg gtttgatatc acagttgcca
    23701 gcgaaatcat ggctatcttg tgtcttgcgg cagaccttga agatttaaag gctcgtcttg
    23761 caaggatcgt tgtcggctat acatttgacc gtcagcctgt gactgctgga gacttgaaag
    23821 cagaaggcgc tctggcatta ctgctgaaag atgcaatcaa gccgaatctt gtacaaacaa
    23881 tttacggaac accagctttt gtccacgggg gaccgtttgc caatatcgct catggctgca
    23941 atagtatttt agctaccaag acagcattga gattagcaga ttacgttgtc acagaggcag
    24001 gatttggtgc agatcttggc ggagagaaat ttttagatat caaagtacct aacttgaaaa
    24061 aagcgccgga tgctgtggtg attgtcgcca ctgtccgtgc attgaaaatg catggcggaa
    24121 tgaaaaaaga tgaacttaaa aatgagaatc ttgatgcttt gaaagtaggt tttgcaaatt
    24181 taaagcgcca tatacgtaat atggaacaat atcagctacc tgtgatcgtc gcaatcaatg
    24241 aattcgtaac agatacagac aatgaactca cactgttgga gcacttgtgt gaaaatcagg
    24301 ggattcttgc caagagagcg agtgtatggg cagatggagc agaaggcggc gttgatttag
    24361 caaaagctgt agtacgattg attgatagaa aagaagctaa ttacaagcca ttataccgcc
    24421 tagaagaaac gatacaagaa aagacagaaa caatcgtcaa gaaaatttac ggaggaaatg
    24481 gtgttgtttt cagcaaaaaa gcgcaaaaac aaatagaaga gtttacgaag aatggatggg
    24541 acaaactgcc gatttgtatg gcaaaaacac agtattcatt ttctgatgat ccttcactgc
    24601 taggtgcacc ggaagatttc accatcacga tccgcgaaat catcccaaaa ttaggggcag
    24661 gatttttagt agccttgact ggagatgtta tgacgatgcc tggtttgcct aaaaaacctg
    24721 ctgcattgaa tatggacgtg acaaatgacg gagaagtgtt gggattgttt taagcttgat
    24781 tataatctac catcgaagga gctgtatttt gactacagct tcttttttta gacgataggg
    24841 aattaatttt gtttacacta ggatcattct ttttctttat tgtattttga gctatactaa
    24901 agcataatag gagggtatga tgaaaacgac atttataaga aaaaccggtt tttacggtat
    24961 gggtagctcg attcagctaa gaatcaacaa tgaaaaacgc agtgttctca gtcatgaaca
    25021 aaagataacg cttgatctgg aagtgccctt tacgatgcag gtcacctttt attggctaaa
    25081 aagccccgtt tatacagtga aagaagcaaa gcctgcttat gtgatcacaa tgaattactt
    25141 gattcttcaa atctatccgt ttttatttct agcaactggg ttgggggccg ttttcctaca
    25201 aaatttattt ttcagttttt ttgcagttat tgtgttgatt gcgttctttt tctatataaa
    25261 aaaccgggca tatttgatca aggaggaaaa ttatggcaaa ctctgatctt tttttaatca
    25321 gtttcaatcg aatcgaaaaa tggatgagag acgagatggg aaatgcacga aatatgggat
    25381 ttaccgaact tgtcagacgc ctagctcaaa gaaaacaatt aatgatcaga aaatacgaag
    25441 atgatctttt acagcttgct cagttaagga atgcgatcgt ccatgatcgg atcgcagtcg
    25501 attttgtcat tgcagagcca aatgattgga cagtcaagcg aatccagctg atcgaacaag
    25561 aactgattca gcctgaaacc gtgctgcctc gatttgcaaa acgtgtaaca ggttttgaac
    25621 aagatcttcc gttacttgaa ctgttaaaga tagtagcaga aaaaaggtat tcacaatttc
    25681 cgttatacaa taaaggaaaa tttgtagcat tgattacttt acgtaatatc gggttttggc
    25741 tagcaaaaga aagccaaaaa ggtccggttg atttaacaaa taaacgagcg ttggatttga
    25801 tcatccaaaa cggaaagtac acaaattatc attttgttcc agctaaaaca catatttacg
    25861 aggttgaagc gatgtttcgt gagcaaggga ctttggaagc cgtacttatc actaaagatg
    25921 ggaatcccga tggtaatctg ctcgggatcg tacgacctcg tgatatttat aaacaaatag
    25981 aaaaggattg attttttgtt aattatttat tggatattca gcgctttgct agtaggagtg
    26041 gatcagttgg tcaaatggtg gatcactgat aatctagcgc ttggagagac gaaaaatctt
    26101 ataccaggta ttttgtcact gaatcatatt cgaaacactg gagccgcatg gagtatgcta
    26161 gaagggaaga tgtggttctt tactatagtg acactcattg cagtcgttgt gattctcaca
    26221 ttgatgatta aaaatcgtga aaaaggtaat cgctggttca tgattggatt gagtttgatt
    26281 ctagctggtg cgattggcaa ttttatcgac cgtattcgtc ttggctatgt agtagatatg
    26341 ttccagacag attttatgag ctttccgatt tttaacgtag cagatgtgac acttgtatgt
    26401 ggagtgattt gtatgttaat ttatattatt ttagatgaaa aggatcaaag aaaaaaatga
    26461 cagaacttaa aacgacgatt caccatgaat ctggtaggat cgataaagta ttgactaatt
    26521 tattcaccga ttacagtcgt tcgcaaattc agctgtggct gaaaaatgga gcggtttctg
    26581 tcaatggaca gccagtcaaa gcaaattaca aagtaaaaca aggagacgag attactgtct
    26641 tgatccccga accagaaaca ttagatgtgg tcccagaaaa tatcccgttg acgatcgttt
    26701 acgaagatga agatgtagcg gtgataaaca aacctcaagg aatggtcgtt catccttcag
    26761 caggtcatac gcatggcaca atggtcaatg cgctgatgta ccatatcaag gatctttcta
    26821 cgatcaacgg cgttattcgt ccaggtattg ttcaccgaat cgacaaagat acttccggac
    26881 tgctaatggt tgccaaaaat gaccaggcac acgaatcatt agctcagcaa ttaaaagata
    26941 agacctcttt gcgcaaatat gttgctttag tccatggaga gattcctcac gaaaaaggaa
    27001 ccatcaatgc gccaatcggc cgttcgaagg aaaaccggaa aatgcaggcg ataagagaag
    27061 atgggaagtc ggctgtcaca cattttactg tattggaacg tttcgaagga tttactctgg
    27121 ttgaattaaa actagaaacc ggaagaacac accagatccg tgtgcatatg aagtatatcg
    27181 gttatccgtt agctggagat ccagtatacg gaccgaagaa aactttgaaa ggcaatggtc
    27241 agtttctcca tgcgcaaatg ttaggtttta cgcacccgac tacaaacgaa aagatgatct
    27301 ttgaagcacc tcttccagaa gtttttgaaa aaactttaga aaaattaaga aaaaacattg
    27361 cattttaatt tagataacgg tatagtaatt aacaacgaaa gcataaaact taatagaatt
    27421 cctttaagtt tagtcccgtg aggctaagaa gggtgggagc aaggatacgt atggtgcgcc
    27481 ataagtagat tgctcattga catagtatag ctatcagtgt atcagtatac gctcagtcta
    27541 aacattcctc ctgtcctctt agggcaggag tttttttatg caaaaaagta gggggaatga
    27601 aaatgcaagc aaaagaagta gtagatcagg taaccatgaa gcgagcattg actcgaatta
    27661 cttatgagat tatcgagaga aaccatagta tccaagatat cgtattggta ggtatcaaaa
    27721 cacgaggaat ctacattgca tcaagaattg ccgaacgcct gaagcagcta gaagatatcg
    27781 atattcctgt aggagaactg gatatcactt tataccgaga tgacaaaaaa gaaaatccag
    27841 aagaaccgga acttcattct tctgacatcc cagtttcctt agaaggaaaa gaagttattt
    27901 tgattgatga tgttttgtat acaggacgaa cgattcgagc agcaatggac gcagtaatgg
    27961 attttggcag accaagaaaa atttcattag cagtcctggt cgatcgtggg catcgagaat
    28021 tgccaatccg tgcagattat gttgggaaga acattccaac atctcgggcg gaagagattt
    28081 tagtagagat gcaagagctt gatggacaag atcgcatcat gattttgaaa gaggaagatt
    28141 agaggagaga gaaacatgtc ggagaaaaaa ggatttcata atgatgacgt ggtgttagat
    28201 atccacgatc gcccaacccc aggacattgg gtaggattaa gtttacaaca cttgttcacg
    28261 atgttcggag caaccgtgtt agtccccatc ttagtaggta tcgatccagg tattgcgcta
    28321 gtcagttcag gactaggtac attggtgtac ttatttacta ctaaaggaaa gatacctgcg
    28381 tacttaggaa gcagcttcgc tttcattgca gcgatgcagc ttttgatgaa aacagatggt
    28441 tatccagcga ttgcacaagg agcagtcaca actggattag tctatttgat tgttgcgctg
    28501 atcatcaaaa agatcggttc agcatggctg gataaaatct tgcctccaat cgtcgtcggt
    28561 ccagtcatca tggttatcgg gttgggactt gcggcaaatg cagcaacaaa tgctatgtat
    28621 aatcagggca cgtatgattt caagtatatt gctgtagcgc tgatcacatt aggattaaca
    28681 attttcttca acatgtggct aagaggtttt ctcggattga ttccgatttt actaggtatt
    28741 attagcggat acatcgtagc cttgctgttt ggaatcgtgg atactaagcc aatcgtagac
    28801 gctgcctggt ttgctgttcc taactttgag attccctttg tacaatatga accaaagcta
    28861 tatcttggag caatcacaac aatggcaccc atcgcttttg tgacgatgac agagcatatc
    28921 ggccatctta tggtattgaa taaactaact aaacgtaatt tctttgaaga accgggtctt
    28981 cataagacgt tgacaggtga tggattagca caaatcgtgg cagcctttgt cggaggtcct
    29041 cctgtaacaa gttacggtga aaacattggt gttctggcta tcacacgtgt ccatagcgtg
    29101 tttgtgatcg gtggagctgc agtatttgca gtcattttag gattcgtagg aaaattaagc
    29161 gctttgattt taagtatccc tggaccagtc atctcaggaa tcagttttat cttatttgga
    29221 gtaattgccg caagtggaat gaagatttta atcgagaaca aaatcgattt tgataaaaag
    29281 aaaaatctgt tgattgcttc agttatctta gtagtaggaa ttggcggact cgtctttgaa
    29341 gtaggcacat ttacattgtc ggcaatggca ttagcaactg tacttggcat cattttaaat
    29401 cttatcttac cagaaacttc acgaagcgaa gaaaactaag gaggaaacat tatgatcatc
    29461 aaatcagaac gtatcagctt aaagcattta ttgaccgttg aggcacttac tgatcaagaa
    29521 gttatgggct tgatccgcag agggcaggaa ttcaagcaag gtgctaattg gactccgcaa
    29581 aagcgccagt actttgctac aaacctcttt ttcgaaaata gtactcgcac acataaaagc
    29641 ttcgatgtag cggagaaaaa actagggcta gaagtgattg aatttgaagc aagcacaagc
    29701 tctgtccaaa aaggggaaac actatacgat acagtactga cgatgtcagc attaggcgta
    29761 gacgtagcgg ttatccgtca tggtgatgag aactattatg acgaactgat acaaagtaaa
    29821 acaatccagt gttcgattat caatggagga gacggaagcg gacagcatcc aacccaatgc
    29881 ttgcttgatt tgatgaccat ttatgaagaa tttggtcatt ttgaaggtct gaaagttgcc
    29941 atcgtaggtg atatcactca ctctcgcgta gctaaatcca atatgcagat gttaaaacgt
    30001 ctaggcgcac aagtattctt ctcaggtcct agagaatggt acgacgatga attcgaagtg
    30061 tacggacatt atatgccatt ggatgaacta ctagatcaag ttgatgtcat gatgatgctt
    30121 cgggtgcaac acgaacgcca tgatggaaaa gaaagtttct caaaagaagg ctaccatcag
    30181 gaacatgggt taacagttga gcgtgcgaag aagttgcaaa aacatgcaat cattatgcac
    30241 ccagcaccag ttaaccgtga tgtagaatta gctgattcat tagtggaagg attacagtcg
    30301 cggatcgttc agcaaatgag caatggagta ttcgtcagaa tggcaatctt agaagcagtg
    30361 ctaaatggaa aagcgtaatt ataggaggat gaaaaaatga aaacactcat taaaaacggt
    30421 cagatcaata cacgaaaaaa tatgacgaca ccagcagaaa tttggataga agatgggcga
    30481 atcaaagcaa tcggtacagg attctcagaa gccgaatttg atgaagtgtt tgatgcaaag
    30541 ggacaattga tcacacctgg tttagtggac gtccatgttc atttgagaga gccaggattt
    30601 acatacaagg agacgatcga agctgggact cgctcagctg ctcgtggcgg tttcactact
    30661 gtatgcgcga tgccgaattt aaatcctgta cctgatacgg cagaaaagct gagacaagta
    30721 tatgacatca ttagaaaaga tgcagttgtg aaggtattgc agtatgcgcc aatcacagaa
    30781 aatctgcgaa gtgagaaatt agttgatcaa gaagcactga ttgaagaagg tgcttttgct
    30841 ttcacaaatg acggtgtagg tgtccaaaca gctgggacga tgtatctggc tatgaaagaa
    30901 gctgcaaaaa acaataaagc cctagtagcc catacagaag atgaatcttt attatttggc
    30961 ggcgttatgc acgctggcaa gaaggcagaa gaacttggtc ttcccgggat cttaagcgta
    31021 accgagtctt ctcaaattgc tagagatctg ttgctagctg aagcaactgg tgttcattac
    31081 catgtttgcc atgtttctac taaagaaagt gtccgtgtaa tcagagacgc aaaaaaagca
    31141 ggaatccacg taacagcaga agtttcaccc catcatctta ttttgatcga cgaagatatt
    31201 cccgaagatt tcggcttttg gaaaatgaac ccgccgctaa gaggacggga ggacagagaa
    31261 gctttgatcg aaggcttgct tgacggtacg atcgattgca tcgctacaga ccatgccccc
    31321 catggattag aagaaaaaag ccaaagtttc atgaaatcac catttggtat cgtaggaagc
    31381 gaaacagcgt ttcaactgat ctatactcat tttgtagaaa ctggtcgatt cacgttggaa
    31441 caagtgatca attggttggc agttaaaccg gctgagattt ttggtttgaa tgcaggaacg
    31501 ctaacggttg gtgcccccgc tgatgtagca gtttttgata ttacacaaac ttgcacgatc
    31561 gataaagaag actttttatc aaaaggggag aatacacctt ttatcggctg gaaagtaaaa
    31621 ggcgaaacac aaatgacttt tgtcaacgga aaacttgtgt ggcagaaggg agaataaaag
    31681 tatggaacgt ttgcttatat tagaagatgg aacagttttc gaaggaaagg cttttggtgc
    31741 agatgctagt gtcttcggag aaatcgtttt tacaacaagt atgactggtt atcaagaaac
    31801 tatcactgat caaagtttta acggtcaaat cattacattt acctatccta tggtgggtaa
    31861 ttatggggta aatagagatg attatgaatc gatttctcca acatgcaaag gagtagtagt
    31921 aaaagaacat gcacgtgtag cttcaaactg gcgaaaccaa atgacgctag attcattttt
    31981 gaaacaaaaa ggaattcctg gaatatcagg tatcgatacg cgcgccctta ctcgcaaaat
    32041 ccgtgaagtc ggaacgatga aagctagtat agttgatgct ggagattcct tcgaacatgc
    32101 atttgatcag ctgaaagcaa ccgttctagc aaccaatcaa gtgcagcaag tctcaactaa
    32161 caaaccttat ccaagtccag gaacaggtcg gaaagtcatt gtcattgact tcggcttgaa
    32221 acatagtatt ttacgagaat tgagtaaaag aaactgtaac ctcacagtac ttccctataa
    32281 cacagatgcc gagacgattt tagcgctatc accagacgga gtcatgctga cgaatggacc
    32341 tggtgatcca aaagatgtgc cagaagcaat cgaaatgatc aaaaaaatcc aaggaaaagt
    32401 accgattttt ggtatttgtc tcggtcacca gttatttgct ttagccaacg gtgcagatac
    32461 ctataaaatg aaattcggac atcgaggatt aaatcacccg gtacgagaag tagcgaccgg
    32521 aagaattgat tttacttctc aaaatcatgg ctatgcagtc gatgaagcca cgattgatcc
    32581 agaaaaactc atcgtaactc atgtagaagt aaatgacgga acagtagaag gagtcaaaca
    32641 cagagattat cccgctttca gtgtgcagtt tcatccagat gctgcccctg gtccgcatga
    32701 tgcactacat ttatttgatg aatttatgga aatgatggac gcaggaaagg agcagtaaac
    32761 agatgccgaa acgtacagat atcaagaaaa ttatggtgat tggatctggt ccgatcatca
    32821 ttggtcaagc cgcagaattt gactacgcag gaacacaagc ctgccttgcc ttaaaagaag
    32881 aaggatatga agtggtttta gtaaattcta atcctgcgac gatcatgacg gataaagaag
    32941 tggcagatca agtatatatc gaaccgatca cacttgaatt tgtatcaagg attcttcgaa
    33001 aagagcgtcc agatgctctc cttccaactt taggcggaca gacaggcctc aatatggcaa
    33061 tggagctggc agcatcaggt attttagaag aattgaacat agaacttcta ggaacaaagc
    33121 tttctgcgat cgatcaagca gaagatcgtg atctattcaa acagttgatg aaagaattga
    33181 atcagccaat ccccgagtct gaaatcgtca atacagtaga agaagctgta gattttgcaa
    33241 ataaaatcgg ctatcctgtc atcgttcgtc cagcctttac tttaggcgga actggtggag
    33301 gtatgtgtga aaatgaagaa gaattacgta tcgtatcaga aaatggatta agtctttccc
    33361 ccgctaccca atgtttgatt gaacgaagta ttgcaggttt taaagaaatt gaatatgaag
    33421 tgatgagaga ttcggctgat aatgcaatcg ttgtctgcaa tatggaaaat tttgacccgg
    33481 tgggtatcca tacaggtgat tctatcgtat ttgcccctag tcaaacattg tctgaccatg
    33541 aatatcaaat gcttcgtgat gcttctcttt cgattattcg agcacttaag atcgagggag
    33601 gctgtaatgt ccaactcgct cttgatccac atagttttaa ttattatgtg attgaagtca
    33661 atccgcgtgt ttctcgttca tctgcgttag caagtaaggc gactggctat ccgatcgcta
    33721 agctagctgc taaaatagcg cttggattaa cattagacga aatgaaaaat cctgttacag
    33781 gaacgacttt tgcagaattt gaacctgcat tagattatgt tgtagctaaa attcctcgct
    33841 ggccgtttga taagtttgaa aacggagaac ggcttttagg cacgcagatg aaagcaactg
    33901 gtgaagtgat ggctatcggt cggaatatcg aagagtcttt attgaaagct gtgcgttctt
    33961 tagaaatcgg tacacaccat atcgaacttc ctgagttggc agacatcacg gacgatgacc
    34021 taatccaaaa agtggttcga gcacaagatg atcgcctgtt ttatgtagct gaagctatta
    34081 gaagagggta caccatagaa gaattagcgg atttgacaaa aatcgattta ttctttttag
    34141 ataaactgtt gcatatcgta gaaatcgaac agcagttgaa acagcagccg ttggatgaca
    34201 agatattgaa aacagcgaaa caaaacggtt ttacagacaa aaaagtggcg gagctgtggg
    34261 aaataaacga agaagccgtt cgccagatga gaaaagaaaa tgatttgatt cctgtatata
    34321 agatggttga cacttgtgca gcggaattcg aatcaaaaac accttatttt tatagttcct
    34381 atgaatatga aaatgaaagt tcacgatcag aaaagccctc aattctagta ctgggatctg
    34441 gtcctatccg tatcggacaa ggggttgaat ttgactatgc gactgtgcat tctgtcaaag
    34501 caatccagca ggcaggatac gaagcaatca tcatgaacag caatcctgaa acagtttcga
    34561 ctgacttttc tatttcggat aaattatatt ttgagccatt gaccttcgaa gatgtgatga
    34621 atgtgatcga gctagaacag ccgataggag taatcgttca atttggtggt cagacagcaa
    34681 tcaacttagc cgaacctttg actgctgctg gtgtaaaaat cttagggaca acgatcgaag
    34741 acttggatcg tgcggaaaat cgtgatttat tcgagcaagc gctgaaagag ttggatattc
    34801 ctcaaccttt aggggatact gctacaagta aagaagaagc cgtggttatc gcttcgagaa
    34861 ttggttatcc agttcttgtt cgtccaagct atgtattagg cggacgtgcg atggaaatcg
    34921 ttgaaaacca gcgagattta gaagattaca tggaacatgc tgtgaaagca tctccagaac
    34981 atccagtact agtggaccgt tacttgcttg gtggtgaatg cgaagtggat gcgatttgtg
    35041 acggtgaaac agtactcatc ccagggatca tggagcatat tgagcgtgct ggtgtccatt
    35101 caggggattc aatggctgtg tatccacctc aaacattatc tgaatcgatc aagaaaacga
    35161 tcgaagatta cacgatccgc ttagcattag gtttgaattg tgtcgggatg atgaatatcc
    35221 agtttgtcat ccatgaaaat gaggtttatg tgatcgaagt caatcctcga gcaagtcgga
    35281 ctgttccatt cttaagtaag gtgacaggaa ttccaatggc acaaattgcg acgaaagcta
    35341 ttttgggaga gaaactaaag acacttggtt atgaaaacgg actttatcca gaaacaaaag
    35401 aagttcatgt taaagcacct gtcttttctt ttacgaaatt gcagaaagtt gatacttact
    35461 tagggcctga aatgaaatcg actggagaag taatgggatc tgacagacaa ttagacaaag
    35521 ctttgtacaa ggcattcgaa gcttctggat tacacttgcc tgaatatgga gccgtattat
    35581 ttacgattgc ggacgaaaca aaagaagaag cactagaatt agctaaacgt tttacagaaa
    35641 ttggctatag tctgatagga acgaaacata cagctgctta tttcgaaaaa aatggtctgg
    35701 tcgttgaaac agtagcgaaa atttctgagg aagcagcaga aaaaaatgta gtcgacttaa
    35761 taagagatgg taaaacacaa gtggttgtca atacgattga taaagatcgg ggaaatgctt
    35821 ctaaagacgg tttcatcatc cgaagagaag cagttgaaca tggtattcca ttgtttactt
    35881 ctttagacac agcagatgcg atcgttcgag tgatggaatc ccgtgctttt tcaactaacc
    35941 ctatttaacg gcgagcacat gttaagacaa aaagacgaaa aaccgttgca ggaggggaca
    36001 gatgaaacaa gaaatgatgg aaatcgttca gcaaaaacag ctggcaccaa agatttttga
    36061 aatgacattg aaaggtgatc tagtaaaaga aatgcagcaa ccaggacagt tcttgcatat
    36121 tctcgtgccg cgtgcagact tgctattacg ccgaccgatt agcttgaatc atatcgacca
    36181 cgaaacaaat acttgccgta tcatttatcg ggtggaaggt gaggggacta gagtcttctc
    36241 tactctttct gccggagacc atttagatgt catgggtcct ttaggcaatg gttttgattt
    36301 gtccaatctt catacaggag acgaagtatt tataatcggt ggaggaatcg gtgtccctcc
    36361 tctatatgag ttatcaaaac agttagtcga aaaaggaatt aagccaacgc attttttagg
    36421 ttttgccaca catgaagtcg tttattatga aaaagaattt aatcgtctag gagaaacaag
    36481 aatcgctact gacgatggaa catacggtat gcacggaaat gtcggtaatt tattgttagg
    36541 aacgaagacg gaacctacag ctgtttttgc atgtggttca aacgggttat tgaagacagt
    36601 ggaacagctg ttttcttcgc atcaaaatgt ccagttatca ttagaatccc gcatggcatg
    36661 tgggataggg gcttgctatg cttgcgtttg tcatacgccg gaagatgaaa caggaacaaa
    36721 aagtgtgaaa gtttgtgacg aaggaccgat ttttaaaata ggagaggtaa ttatatgaat
    36781 gagaatcctt tagcggtcaa acttcctggc ctagaattaa aaaatcctat catgccggca
    36841 agcggctgtt tcggttttgg caaagaatac ggaaaatact atgatttgaa tcagcttgga
    36901 tcgatcatgg tcaaggcaac gactccaaat gcccgctttg gcaatcccac gccaagagtg
    36961 gcggaaacac catctggcat gttgaatgca attggtttgc aaaatcctgg tctagacgtt
    37021 gttatgcata aacttcttcc agaattagag gagtacaatg agttacccat catcgcaaat
    37081 gttgctggtg cttgtgaaga agactatgta gaagtttgta gcaaaattgg cgaagcacca
    37141 aatgtgaaag caatcgagct gaacatttct tgcccaaatg tcaaacatgg cggaattgct
    37201 tttggaacag attctgatgt tgcttttcaa ttaacacaag cagtaaagaa agtttcttct
    37261 gttcctgtct atgtcaaatt gtccccaaat gtaacagaca tcgttccaat cgctcaggca
    37321 atcgaagctg gaggggcaga tgggtttacg atgatcaata ctcttcttgg tatgcggatt
    37381 gaccttaaga caagaaaacc gattttagcc aatcaaacag gtggactctc cggaccagca
    37441 atcaagcctg tcgctattcg tttgattcac caagtagcaa gtatatccag ccttccaatc
    37501 attgggatgg gcggcgtgca gacagtggat gatgttcttg agatgtttat ggctggagca
    37561 agtgctgtag ctatagggac cgcgaatttc accgatccgt atatctgtcc taaactaatt
    37621 aaagagctcc ctgtgcgaat gagcgaacta ggcatcgaat cgctggaaag attaatcaaa
    37681 gaagtgagag aggaacgtga acgatgaatc aactagacca acgaccaatt attgcattag
    37741 atttttcaac taggcaagag gtagaagact ttcttcgatt tttcccgaaa gaagaaaaat
    37801 tgtttgtgaa aatcggaatg gaattgttct accaagaagg acctgaaatc gtacgttatc
    37861 taaaagaggc tggtcataat gtatttttag atctgaaact tcacgatatt ccgaatacgg
    37921 tcgaaaaagc aatgcgggga ttggcaaagc ttggtgttga tctcacctgt gttcatgccg
    37981 ctggcggtat tcgtatgatg gaggcggcaa tgcgaggatt agaagagggg actccagaag
    38041 gtggaaaacg tccgctactt ctagctatca ctcagcttac ttctacaagt gaagaagaaa
    38101 tgcatgccga tcaactaatc gaagtttctt tagaaaaaag tgtgatccat tatgctagct
    38161 gtgccaaaaa agcaggatta gatggtgtgg tttcttctgc ttgggaagta gaagcgatca
    38221 aagaaatggc aggagacgaa tttgtttgct taactccggg tataagacca gaaggaacag
    38281 tcgcaggaga tcagacaaga gtagtcacac cttctcaggc aaaaaagatc ggctcaactt
    38341 ttatcgttat aggtcgacca atcactcagt caactaatcc ttataaagca tatcaaacga
    38401 ttcaaacaga atggagctaa cctaaaatga atgttgaaca aagtatcgca aaagacttat
    38461 tagaaattga agcagtattt ttaaatccaa gtaatccttt tacatgggca agtggaatca
    38521 aaagcccaat ttactgtgat aaccgaatca cgatgagcta tccaaaagtc cgaaaagaga
    38581 tcgcaaaagg attagcaagc aaaatcaaag aagcttaccc tgaagtacaa gtgattgctg
    38641 gaactgcaac agcaggaatc cctcatgccg catgggtagc ggaaatactg gatttgccaa
    38701 tggtctatat tcgaagtaag gcgaaagatc atgggaaagg taaccaaatc gaaggacgta
    38761 tatttgaagg tcagaagatg gttgtgatcg aggatttgat ttctacaggc ggaagtgtct
    38821 tggaagcagc tgaagcagca aaaagagaag gtgcggatat ccttggtgtt gcggctatct
    38881 ttacgtatga gttgccaaaa ggaaaagcaa atttcgaaaa agccgaaatc cctcttatga
    38941 cgttgaccaa ttattctgta ttgattgaag cagctttaga agatcgttat atcgatgagc
    39001 aagaattaac tctgctaaaa gaatggaaaa aagatcctga aaattggcag atttaagcgc
    39061 gcttgatgaa gatagagaaa tccgatttgt gtctccagaa tcatcaattg gaatagcaaa
    39121 aatccaaaaa ctgattgcga gaagcatctt ttcatcaggt atgaaaagaa acttctatcg
    39181 tgatcagttt ttgatttttt taaaattcat ttttctaaga ctggttagca gaaagaagag
    39241 aataatctct tctctttttt ctattatata tagattttct ctattttttg gtaagggagc
    39301 tgtttcaaaa atggtaaaga gcggaaatgt tgcgaatatc agttatttaa tgatatgata
    39361 ttcggaaaaa gaaatctcaa agggaggtaa cttttatggg gttgaatgaa tttattttag
    39421 gattgaacaa tttagagacg atcacaaggg cacctggatt ttttaaatat acagagcata
    39481 cagttgcagc acattcgtat cgtgtagcat ctattgcgca agttctagga gacattgaag
    39541 aagcgaatgg acaaccgatc gattggaaac tgctctatga aaaatcattg aatcatgact
    39601 acacagagcg ttttattggt gatatcaaaa cgccggtgaa atacgcaagc aaagagttaa
    39661 gagggatgct gcaaacagtt gaagaaaaga tgacggacga attcatcaac caagagattc
    39721 cagctgagtt ccaagaagtt tatcgccgtc ggttgttcga aggaaaagat gaaaccgtag
    39781 aaggagaact attagccatt tcagataagg ttgaccttct ttatgaatct tttgatgaaa
    39841 tcatcaaaaa taacccggaa aaagtttata aggagatgtt cattgaggca gtgactacga
    39901 ttaaaagctt tgaacatcgg ccttctgttc agtatttctt taaagagata tttccggaac
    39961 tattgaacca ggaattttat ggaaaagagg cgtttttaga aaccgtctca acgattgtaa
    40021 agaaagaaga aaaataaaaa tgatatgaga tgcaggggga agtatagttg aaaaagaata
    40081 tggatgtcga atggggctat tctggagaca aaggtccgga aaattgggcg agtttatgtg
    40141 attggtttgc gagaggtgcg gaatttccat tgcagtcgcc tattcatttg accaaggggg
    40201 aagaaaccaa aatagaagga gacacgcttt cctttcatta tcaaaagcaa cggtttactg
    40261 acaaggaatt caaaaacacg attcatttag taccttttga ccaactcagt tatgtagagt
    40321 tcaagaaaga acgctattat ttgacagata ttcatttcca cttgccttct gaacatatca
    40381 tcaacggaaa acaggaagat attgaatttc attttgttca tatgaacagc aaaaaagaga
    40441 atctagttgt gggtgtcatg taccagctaa tggtacagga aggttggctg tataatcaag
    40501 aaaatgggga aagttggaac ttagaaaaac atgagcattg ggtaaatccg gatgtgttct
    40561 ttccggagca aaaaagccat ttccattata ttggctcatt gacaactccg cctaccagcg
    40621 ggccaatcca atggtttgta atggatcatg taggaaaact gaaccaagag tttcttctcc
    40681 ccttcgatgg tcagtatgct cgaccgaaca accgaccaat ccagcccttg aatggaagag
    40741 aaatttatta tttcgaggag tttgagcaat gaatattcag cagatgaaat acgtggtcgc
    40801 tgtagcaaac aatggtagtt tcagagaagc ggcaaagaaa atgtttgtct cccagccaag
    40861 tctttcagct ggtatcaaag aattagaaac cgagttaggg atcacgctgt ttactcgaac
    40921 gaatcgaggg gcatatttaa cagaagaagg gcaagacttc ttaaatcgtg ccgagaaaat
    40981 attggtccag cttgatacat tagaaactca ctatctaacg agtgaaaaga gagacaattt
    41041 ttcgatcgct gcccagcatt atgatttctt aggtcctttg accgcacagc tactcgagga
    41101 attcaaagac gagatcaaac aatttcggat catggagacg acaacagcga gagtgatcga
    41161 agaagtcaaa gagcagcaca gtgagatcgg cattatttat atgaatgagc ataatcaagc
    41221 tggtatcaga agatatttac aacaaggaga gctaagtggc cgcagcattg gaaaattcaa
    41281 aacgcatatt tttttaaggc aaggacatcc attagctgaa cacgcagaga tcaccaaaga
    41341 agaattgtta tcttatcctc aagttcgttt tacacaggat ggaaataatt tcccgtattt
    41401 ttatgaagac cttatcgaga tccctgatca agaaacagtg atttatacga gtgatcgagg
    41461 tactttgatg aatatcgttc ttgaaactga tgcttacgct tcaggatctg gtattgtgat
    41521 tggagggatt cgcgagcact tgcggttaat ccctttggca gaaggcgaac taaatgaatt
    41581 ttatatcatt tattcggcga aacgtccgct cagtgaaatc gctcaaagat ttatcaaaga
    41641 gcttactcgg ttactggacc agcagagcta agaaaagcag atgagaacgc tgtttattga
    41701 acaccgttga tcaaaaaaga ggggttgtga cagaattttg tcacaactcc tctttttgta
    41761 gcaaacaatt tataactaat tgctgttgtg ggaaagtacg caaatatgat tctagaaaca
    41821 ctcattgatt acctgttttg tttcatagct gtaatttttt cttcttctgg tgtcacgata
    41881 atcgtctttt ggttttcata aataacataa ccaggcttgg cgccatttgg ttttcggata
    41941 tgctttacct gtacaagatc aaccggcact tgggcagagt atcggtattt tgaaaaatag
    42001 gcagctaact cggcagcttc tgtaattgtt tcatctgagg gcttgtcact tttaatgatg
    42061 acgtgcgaac cgggtatatt ttttgcatga agccaatagt cggttttttt agctgtcttc
    42121 attgtcagct ggtcattttg aagattgttt ttaccaacaa gaatgagcgt tccgtcagaa
    42181 gaaaagtatt gatcaggctg agatggtttc ttacgttttt gtttttttaa gttcttcttt
    42241 ttcaggtatc cttctgaagt caattcttct ttaattgctt cgatatccat tgggccggca
    42301 atttccagtt gagataagac agattcaaga tattggattt catctttggc ttcttggatt
    42361 tgttcgccaa tcagcttaac agcgttcttc agcttttggt atcgatggaa atatttctgt
    42421 gcattttgat ttggtgtcaa tgccggatct agtgctattt tgattggccg atcttcttca
    42481 taataattag gtaaaacaac ttcttttgcg ccgcgtggta cttgtgtcag aaaagtagta
    42541 agtagttcgc catttctacg gtaattctca gcgttttcgg attctttgag tgtttgttca
    42601 cgttttttca atttattttt atttcgtttt agttcatttt cgatttttcg aatcagttct
    42661 ccgccttgtt gttttgcccg atctttctct gctttttcgt gataatagct atctaataat
    42721 tgactgagtg ttggataagc tgttacagca gatgcctgtt cggacaatac ctgataagca
    42781 atcggtgtga aaaaatcttt attttccgtt tcgtaaaaag taggaatagg ctgatggctg
    42841 atggctgaga aaaattcgtc ccatacaacc atcttttcat taggacgttc cagtaatctt
    42901 ttggtcagct cttgagcgat atcgaaaccg attccctgaa actgacgttg gattgctttt
    42961 ggatctagtt cagtttggga aagccgctga aagatttttt ctttctcgga gctaaatggg
    43021 ttcaagacct cttgttttgg cggagcaaca tattctacac caggaagcaa agaacgataa
    43081 gtgttttgag agcttccaat gtgtttgatc gcatccagta tttttccagt ttctcgatta
    43141 actaagacga tcgtactatg acgtcccatt aattcaacga tcaatatgat attttgtaga
    43201 tcacccaact cgtttcgttt agcaaagtgg aaatggatca ctcgatcatt ttcgatttgt
    43261 tcgatgtctt ctaaaatagc gccatccaaa tattttcgaa gcatcatcac gaagttaggc
    43321 ggattatcag gattctgata atcgatttgg gtaatctgta cacgagcata acttgggtgc
    43381 gcagataata ataatcgatg gtttttgccg cgtgaacgaa tcaccagcac gatttcattt
    43441 tcataaggct gatggatttt cgagatccgg ccagatagta acgtttcacg caattcgttg
    43501 accatcgcgt gggtaaatac accatcaaag gacatggttc attcctcatt tcttttcata
    43561 agtatattca ttataacgaa aaaaagaaga aaaaccaatt tggccgaata gtaaagcaaa
    43621 agtctagaaa aatcttggac taattacata aaaaacagtg tttctatcgt tgaaatccaa
    43681 atttttaggt ataataaaaa ataggagaaa aagtagaaaa gaaggtgttt gttatttggg
    43741 aagtagttaa atcgagagta caagaaatga atgaacattt ctcagtgtca ttcgcagaaa
    43801 atccaagtaa gtattttcga cgtcttgttt ttctgctcat cttatcattg ctgttaacag
    43861 ttgcaatgtt tttattgccg gaaaaaatcc gagaagagca agtaaatagt ctcgtttatt
    43921 cgtccatacc aaccagcaaa ttgaaacctt ttgattacag caaagctgat cagaaaatca
    43981 aacaaagccg ggcgatatcg gtgatgttca gcaaaccgaa cgggaaaacc tatcaggaca
    44041 tgcttgatgt ttttaatgat cctgagctta tggaagaatt gaatcgaccg atcttttatt
    44101 atccgatcgt ttatgatgta catgaactgg aacaaaaata taacatacga acggatgaag
    44161 tgacatttat tttctttgat ggaggaaaag aagcaaatcg gataactgcg ggtaagggag
    44221 gaataactga ttttgataag gagttgatcc ctgaactgaa tcgacttccg ttagccaata
    44281 tcaaacaatt agaagaagaa ttggatagaa cgggagtaac ttcccaaaat tccgagattg
    44341 ctaattgaga cattggcgcg atgaaacggt gggtctatat agcgctattt ttaattatca
    44401 gaacaagtga tgtttgatct tcaaaataac aaataaaaaa ttaaaaaata tagttgacga
    44461 aattcaaaaa ttcgattaga cttaaatcaa ttcttaagga aagggtgacc tgtaatgaac
    44521 ttatcaaaac gtatttcagc agaaaacaaa aaaaattggc gcagctggcg tagataagtt
    44581 gtattaatgg tgagactttc ccatagatac aactttttag agtgcatttc taaagcttgt
    44641 atctatgcta gacatgaaca ttgactgccg catagattag aatctatgcg gttttttgtg
    44701 taaagacaat gaccgctttg gccattgtct ttttttgtag actggatact tgcctgatta
    44761 taataaataa aaagattgac tttgcaggag gaaaaaaatg aaaaacaaac gattaataac
    44821 agttgtagta attattttag tttatttagc aggtgcattt atttatggaa aagcctcagg
    44881 cgaaaaagaa agtacttcga cgaaccagac aaaaaaaaca acagttgttg gggtattgca
    44941 atacgtgagc catccggcat tagatgagat ttatcgagga atcaaagatg gtctgaaaga
    45001 agaaggatta aaagaaggag aaaatcttga gatcaaattt caaaatggac aagccgatca
    45061 gagcaagcta gccacaatga gtcaacagct agtacaatct gatccagatg ccttgattgg
    45121 aatcgcaaca cctgccgctc aatcattagc aaacacgact tcatcgatcc cagttgtgtt
    45181 aggtgctgta acggatcctg tgggggctgg attagtcgct tctttagaga aacctggtgg
    45241 taatgtaaca ggtgtttctg atcagccgcc tgttacttca caaatcaagc ttgcttctga
    45301 tttgttgcca aatgcaaaaa aagtcggaat cttgtatgct tcttcagagg ataattcaaa
    45361 ataccaagta gcacaagcag aagaagctgc aaaaaaagaa gggttagaat cagtaaaata
    45421 tgcggtgcct tccactaatg agattgcaca aacggttcaa gtgatgagcc gacaagtcga
    45481 ttttatctat gttccattag ataataccat tgccaatgcg atgcaaacag tggtaaaaga
    45541 agcaaacaaa gcaaatatcc ccgtcattcc atctgtggat acaatggtgg aacaaggtgg
    45601 actagcaaca atcgggatca atcaatatca attagggctt caaagcggta agatggcggc
    45661 agaacttgcg agtggaaaag aaaaaccgga aacgacgcct gtttatacat ttgatcaagg
    45721 ggatacagtg atcaatcaat ctcaagctga tcgcttagga atcattgttc cccaatcgat
    45781 gaaggaaaaa gccaaaatca ttacagatga ctcgcagcaa gaaacatcta aggaggatga
    45841 caaatgatcg tatcagctat ttcacaaggc atgctttggg caatattagg attagggatt
    45901 ttcatgactt atcggatctt agactttcct gatatgacga cagaagggtc cttcccatta
    45961 ggtgctgctg tctgtgtgac agctattact catggggtag cgccgattgt agctacatta
    46021 ttaggcgtag gtgctggcat gatagcagga ctcgttacgg ggttacttta tacaaaagga
    46081 aagataccgg ttattttagc agggatttta gtgatgtcag gattgaactc agttattttg
    46141 tttgtgatgc gaacaccaaa tctttcttta ttgaatcatc cgattctaca agacagcttc
    46201 catcgctttg ggttacctga ttactttgac acagtccttc ttggcttcat ctctttgggc
    46261 atcttgattg ctgtactgct attcttcttc aatacagatt tgggtcaagg ctatattgcg
    46321 acaggagaca acgaaacgat ggctcggtcg ttagggataa aaaccgatcg tatgaaaatt
    46381 ttaggtttaa ctttgtcgaa cggtgtcatt gcattatccg gtgcgctgat tgcccagaac
    46441 gacggatatg cagatgtaaa taaagggatt ggcgtaatcg tcatcggtct tgcttccatc
    46501 atcatcggtg aagtgatctt ccaagaattg acgctagcag aaagattgat tgctattgtt
    46561 gtcggcagta tcatttatca gctattgatt ttagtagtaa tcaaactagg gtttgacacg
    46621 acttatctga agttgttctc cgctatcatc ttagctattt gtctgatgat cccgcaattg
    46681 aagaaagcac tgaacttaaa aacgggtttt gagaaggagg catagtaatg actgatatat
    46741 tgacaatcaa aaatggcaga aaagtcgtaa acaatggaat gaatgaagag aaagtactat
    46801 tggatgaaat taaccttaca atcagagaag gggacttcat cactgtcctt ggcgggaatg
    46861 gtgctggaaa aagcacactg ttcaatacga tcgctggtac gcttcaactc agtcaaggat
    46921 cgatctcttt taaagatcag ctgatcacaa aagattcaga agaaaaaaga gcacgattcc
    46981 tttctcgagt tttccaagac cccaagatgg gaacggctcc tcggatgaca gtagcagaaa
    47041 atttgctact tgcacaaaaa agaggtcaaa aacggaaatt ccgtttacgt aacttgaaaa
    47101 atcaaaaaga agattttttc agactatgtc aagaagtcgg taatggccta gagcaacact
    47161 tggatactcc agcgggtgaa ttatctggtg gtcaacgcca agcattaagc ttgctgatgg
    47221 cgacgatcac agctcctgag ttactattat tagatgaaca tactgcagct ttagatccta
    47281 aaacagctaa agcattaatg cgattaacaa atcagcggat caatgaacaa aaattgactt
    47341 gtctcatgat cacgcatcga atggaagacg ccttgaaata cggaaatcgt ctgatcgtac
    47401 tccaaaaagg gaaaatcgtt caagatattt ctgaaaaaga aaagcaacat ttgacaatgg
    47461 gcgacctttt gcagttcttt gaagaagtag tggattgatt tcgacagaaa aattcctttc
    47521 agtagaagga cttgaaaact cattgacatc ttcctttgat tcatcctata cttaaaacaa
    47581 taaacaatta aacatatttg ggtgttttgt aggatcacgt agtcaatgat tttaacaatt
    47641 aaatagaata ttctttgctc tgacaagtaa acgatgagaa aagaatttgc tactttgcca
    47701 cctagacgtt tcgtactttt tcgtatcaaa cgctaagtgg ctttttttat tgaagtaaga
    47761 acaaaggagg aataagatga tttctggaca aaccaaatta gctgggctct ttgctgatcc
    47821 ggcttctcac agtttatccc cattgatgca caatactgct tttgaagcaa acaacatcga
    47881 tgcggtgtat ttagctttcc aagtaaatcc aatgaatttg aagcaagcag tcgaaagtat
    47941 tcgaactttt gatatgttag gtgtaaatct ttcgatgccg aataaaacag cggttattcc
    48001 ttatctggat gaggtaagcc aggaagcgca attgatcgga gcagtgaata cgatcgtgca
    48061 cgaaaagggt cgtttgattg gctataatac tgatggtgcg ggcttcatgc aatccgtgca
    48121 agaagctggg atttcgatca aaaagaaaaa aatgactcta ctgggagctg gaggtgcagc
    48181 aaaggcagtc actgttcaag cagcgttaga cggcgctgag gaaatcgtag tttataaacg
    48241 aaacaaccaa acattcgctc aagtaaaaga aacattcgaa caactcagct atcgaacttc
    48301 ttgtaagata aaagttcgtg actttgccga tacgcatcaa ttaagaaaag acttgaaaga
    48361 aagctgtctt ctagtcaatg caacagatat cgggatggga aagaaaatca atcagtcacc
    48421 gctcttagat ataagcatga tgcatgagaa tctagcggtt tttgatttga tttatgcacc
    48481 tagagaaaca cgtttattaa aagaagcaaa aaaagtagga gcaaaaggat acaatggact
    48541 tggcatgttg atctaccaag gagcaatcgc gtttgaatta tggacaaagg aaaagatgcc
    48601 gatccatgtg attgaaaagc tgtttgatta aaaaaggata atagataaag atcttttatt
    48661 ctcgcataaa cggtctcaag aaaagtaatc ctttatgtat cgaagcattt atttcgttat
    48721 tttttattaa attatcaaaa aatgataggc ttttgtcgat tggttatcgt atcctttatt
    48781 tatcagtaag aaacgaggag gaaccacaat gagtcggatg gatcgctata aggacatcca
    48841 taagaaggcg aaacctctga agcgggatac ggggagtatt tttgctagaa gagaacgaaa
    48901 acgaacgcgt atggaaaatg aggaagatgc tacccgtatt tatgaaagag agcagcattc
    48961 atttgaacat ttgaaccaat acgagcaaca gatatacaga gatgcaccaa gagcagaaga
    49021 acaacaaaaa aataaaaaat ttttcaatcg gcaaaagaag aacaaaccgt taaaacctcg
    49081 aagaaagcga tcgtggaaaa aaatcattct atcgattttg cttgttttga tcctttattc
    49141 aggtatctca ttttttattg gtaaatatgt agctgaacat gacacaagct tagaggcagc
    49201 gacttcagaa acctttcatg ggcaaaaagc gacgaatggt gcgcataata ttttgatcct
    49261 tggaagtgat acacgcggag aagatgccgg gcgagccgat acgatcatgg tcttacagtt
    49321 ggatggtcct tcccataagc cgaaactgat ttcttttatg cgtgacagct tcgttacgat
    49381 cccaggggta ggacaaaata agatcaattc agcttatgca tacggaggag ccgatctagt
    49441 gcgtcaaact ttagtagaaa attttggaat cgattgtcaa tactatgcga aagtcgattt
    49501 caaatcgttt gaaaaagtga tcgatgcctt attcatgaac ggagtgaaga tcgatgcaga
    49561 aaaagatctg aatctggatg gtgtagatat taaaaaaggg ggccagaaga tggatggaca
    49621 tgttcttttg cagtacgcac gtttccgaat ggatgaacaa ggagactttg gccgtgtcag
    49681 acgccaacag caagtaatga acgctatttt cagccaattg aagaatccat tgaatctgat
    49741 ccgtgcacct tatgcagctg gtaaagcttt aggatataca tccacagatg tcactgcttg
    49801 gttcttgacg aaaaacctct tttcgattgc acgaggagct ggtggcattg atcgaataag
    49861 tgtgccagta gacggttcgt ggaattatgg gcaaagtgat tatgctggca gtattcttga
    49921 aattgataat caaatgaatc aatctgctat tacagatttt ttgaataaat gatcataaaa
    49981 aagatcaaga agaagctgcg caaggctttt gtcatgaatc aatcaaacga ttcactcgta
    50041 aaaaacgctt tcttatggta gatttaacca agagaggagc tgtttacatt ggatatctat
    50101 ctgaacttca aaaatgagtc gcgtgaagcc atcgcttttt atgaaaaagt ttttcagact
    50161 gaagcatcag gtatcaccac atacggtgaa atgcctggaa tagaacaaat gaacttgcca
    50221 gaagaagcaa aaaatctaat cacgaatgca cggctgaaca tttatgatac attagttatg
    50281 tttgcagata cgccagaagg aattggtcct gaattagttg tgggaaataa tgtttcttta
    50341 gtcatccaga caagaaatga agaagaaacg aagcgcctat ttgaaagctt agctgagggt
    50401 ggtaagattg agatgccact ggatgctgtt ccgtgggctc agttgtatgg gatggtaact
    50461 gatcgatttg gcatcaactg gcagattaac caagagtgac agctaaagat gaatattttc
    50521 tggaaacatc aagcagcaaa cggatcatcg tttgtttgct tgatgttttt gtgtctattt
    50581 cgactatacg gtatcttttt tttattgcca gatgcgaaaa tatgttccat aatagagaag
    50641 tccgcaaaaa attaagtaac ggaggtcaga agatgaagac ggtgatacta gctgaaaaac
    50701 cttcacaagc aaaagcctat gccgaaacct tccaacagca aaagcggcat gaagggtatt
    50761 atgaaatcag cgatccattg tttgctggtg aagtaaccat cacttatggt tttggacatt
    50821 tggtagatat ggttgctcca ggagcttatg aagagcgttg ggcaaaatgg tctttagaaa
    50881 atttgcctat tttcccagaa accttccgtt acgaagttcc aatggacaaa cgtgcacaat
    50941 tttcgatcgt aaaacgagaa ttgcagtcag ccgataccat cattattgca acagatggtg
    51001 atcgagaagg tgaagccatc gcgtggtcga ttattcgaca agcaaacgca ttttctaagg
    51061 aaaagaacta tttacgttta tggatcaatt ctcttgaaaa agaagcaatc tatgaaggtt
    51121 ttaagaatct ccaacctgga aagaactatt ttccaaaata taaagaagcg caagcaaggc
    51181 agaacgctga ttggctgatt ggaatgaatg gaagtccatt atatagcttg ctacttcaac
    51241 gaaaagggat tgatggcagt ttttctcttg gtcgtgtaca aacaccgaca ctctatatga
    51301 tttatcagct gcaagaagaa atcaaacact tcaaaaaaga aacgtacttt gaaggagaag
    51361 gtttaattac gacaaacaaa gggcagtttt ctggaaaaat cgtaccaaaa aaagcattta
    51421 aaacccaaga agagttaacg aaggaaatag aaaaacttgg agcgcattta ggtaagcaaa
    51481 acggacggat tcttgaagtt acgaaaaagg aaaagaggtt gcatagtcca cgtttatttt
    51541 cgctatctag tttgcagaca aagatgaatc agctaatgaa agcaagtgcc aaagctacac
    51601 ttgatgctgc tcaaggattg tacgaaagca agtttctaag ctatccaaga acggattcct
    51661 tttacattac agaaaatgag catcagtatc tggttaaaca tctgcaaaat tataaaaaat
    51721 ttttaggaat agaagaagta cagacaactg agatcaaagc aaagaaacga tatgtcgatg
    51781 cgaaaaaagt tcaagaacat catgcaatta ttccgacaaa gacgattccg actcctgcac
    51841 gctttgcgaa gctttccaaa ttacagcaag caatctattt gcaagttttg cgcactactg
    51901 tggctatgtt tgctgcagat tatatctacg aagagacgac cgtaatgacg ggcgtacagc
    51961 aattaaagct aaaaagcatc ggaaagatcc cattacagca aggttggcag gaaatcttac
    52021 gcatcaagtc aaaaaagaaa gaagctcaga ctcttcctcc tatcgtagaa aatgaagaag
    52081 cagaagtaga actgaaaagt atggagaaag aaacacaacc tccaaaaccc tataccgaag
    52141 gaacgttgat cacagcaatg aaaactgcgg gaaaaacgct ggataatgat gaagcgcaag
    52201 aaattctcaa agaagtagaa ggaataggga cagaagctac acgagccaat atcattgaaa
    52261 atcttaagca gaagcattac atcgaagtca ataaaaacga aataactgtt acgccaaaag
    52321 gaatcactct ttgtaaggca gtagccaatg aaccattact gacgagcgca gaaatgactg
    52381 cgcgctggga agggtacttg agaaaaattg gtaaacaaga ggggacacca tctgtttttt
    52441 tagagaatat caaaaaattc atcctacatt tattggcaga agttcctaat caaatcgaca
    52501 aggtagactt ttcagatgag attagcggga cacgtcagat aaaagcgatg gagaagaaaa
    52561 acgatcaatt aggaagatgt ccaaaatgta aaaagggaat agtcatgctt tatcctaaaa
    52621 tcgctacatg cttgaatccg gaatgcgact tcaaattgtg gccaacagta gctaaaaaga
    52681 agctgactaa aacgaatctc agagatttgc tttcaaaagg aaagacaagt aaagttgtca
    52741 aaggttttac tggtaaaaaa gggaaattcg atgcagtttt agtattaaaa gaagatatga
    52801 caatcggttt ttcttttcca tggatcgaaa acagtggaac aaaagcagtt gatggaaaca
    52861 aagaaacatc aagaaaaaat aatgaaaacg aagtagagta aatgaaaaca atgaaatagc
    52921 ggaatattta ttccgaaaag tgagaatata aataccaata aaactaaaaa atatcagtga
    52981 atcaagcaaa aacgttgggg taggctgatt ttatatagat agcaaggagt ttttttattt
    53041 gaaaaaagtg cattcatttt taaattatct tatgggagca atcacgattg tggctgtgta
    53101 ccaagcattt ctaaagtatc ctgattacgg gacgaagttg ttgtcattga cgcacaatca
    53161 aacagcatat tcaccaacgt tgattttatc gactttgact gcaggagcta aaagcgctac
    53221 ttattttttt cataaaacga caagcatgct aaagcgaaag aaacagcgaa aacaattgtt
    53281 ggtggtggta gcggctatga tcattattta ttttctacca gttattacac aagtatttca
    53341 tcaatatttt ggttaagaat acgagagttt gtgacaaaag tcatgtcaca aacttttttc
    53401 tttgtcattt tttcgtaaga aaaagcaaaa taaaagtata tacattgatt aaaagtatat
    53461 acttggatat aatgtactta ctatagaatg agaatggtat agagggtggc gaaatatgaa
    53521 aaaaatgaaa aagtataaaa ttagaaaatc tggcaatagc gatgtaacaa ctatcccacc
    53581 agaagtaaaa gaattcatgg gtgtgcagac aggtgacgca atcagttatg tttttcaatc
    53641 tgatggttct gtacgaatga tcaaagcaca agaagaacct gatattgatt cattagttga
    53701 ttcgatcatg aatcaatatg aagacgcgtt aaaagatttg gttgatttat gaggtatctt
    53761 tcgactaaag aaattatcaa aatcaacgcg aaagttattt taccgtcact cgccaggcga
    53821 aatgatcggt gtaaaagatg caaatgcatt agatatggcc gtcaaacagc cttcacaagc
    53881 agtattcaat caagaactct atcctgaaat ctatgaaaaa gcggcgattt tagcaattaa
    53941 tttagctaaa aaacatcctt ttcataatgg caataaacga actgctcttg ttgccatgtt
    54001 gttgtttctt caattgaata attgtccagt tgctttttct cgtcaagaag ctgttgattt
    54061 tatcataatg attaccacta gttcattgga tttcgattca ctaaagtcca agataaccag
    54121 ttatttacga caaaccgcaa taaaatgaac gaattaaaaa ctttatttct caattagatc
    54181 agcatgtcat taaggaatat gctgattttt ttatgtttaa tcatacaaaa tagttttttc
    54241 ataaaaatat ggaaaatatg gattaatttt gaaataattg atttaaaagg aatacaaaag
    54301 agtgaatagt aggaaatagt ggtttagaac tagtgggctg attttttata tattttagtt
    54361 tacataatat atattataca aagtagattt aaaaaatgat aaacctctct cgaacggttt
    54421 tttatcaaaa atcgtcattt ttccatcaaa atctgcttat gcaaaattat aaaaaaattt
    54481 tgcagttttt cttacttttt ttgtcgttta attgaatgat aaaaaccagt ttctttattc
    54541 agttaatcaa atatgaacga attagatatg aatcattgta tttgccgttg cggaaggatt
    54601 ttttaattaa ccacagataa acattgataa cgattcgctt attgtaatca acagattaaa
    54661 tttcatatgt tcatatgtac ggatacattt tcggaccgat taattttaac cattctgttt
    54721 atttgcttac ctattagata tcaataaaaa actgcttaac agctatttca gctgttaagc
    54781 agttttagga tttttagttc atattcattg tttcttataa tacagtgttc aatacttctc
    54841 ttaaagcact tgttgatttg ctgaacattg ctttttcttc ttcgttgatt gataattcaa
    54901 caacttcacg aacaccattt tcgtcaacga ttgatggcac acctgtaaag atatcttttt
    54961 cgtcgtattc accagttaaa taagcagata caggcaatac agcttgttcg ttattcaaga
    55021 tagcttttac gatacgtgca gtactcatac caataccata gtaagttgct tttttacgat
    55081 caatgatctc ataagcagca ttacggactt tatcagcaat cacatctaat tcatcttggg
    55141 caatacgatg gtctttttct acaatttcaa acactggttt accaccgaca gttgtatgtg
    55201 accatgcagc aacttctgaa tcaccatgtt cacctaagat atagccgtgg acgctgcgtg
    55261 ggtcaacttt taatttcaat gcgatttctt tacggaaacg agttgtatcc aatgttgttc
    55321 cagtaccgat aacacgtgat gttggcagac cagattcgtt ccaagcgata tatgtcaaaa
    55381 tatctactgg gtttgaagca acaacgataa taccgtcgaa tcctgatccc atgatttctt
    55441 ttacgatttg gcgcatgatt gacgcatttg ttttaactaa atctaaacga gattgaccag
    55501 gtttttggtt gataccagct gtgataacaa cgatatcagc atctttacat tcttcatagc
    55561 cacctgacca tacagccaca ttttcatcgc cccaagccat accatctaaa aggtccaatg
    55621 cttccccttc tgctttttct tgattcacat cgatcaatac taattcatta gagattcctt
    55681 ggttgatcat tgcataagcg atacttgttc caacaaaacc tgtaccgacg attactactt
    55741 tacgacttgt ttttttcatg aataaacagc tccttttcat aactatttgt tactttcatg
    55801 aaaagtatag tcttgtttat tcattttatc aacatgttaa ttataatagt tgttattata
    55861 atttttgatg gaaacgaatt cctgaatacg cataacagat aaatgacaaa tgtttacacc
    55921 tatatcccct gataatacaa ccaaaagttt agaactgaaa ataaatccaa aaaaattcgc
    55981 tgcaacgata taataagaca gcaatcgcac ctaaaacaat tttccttcat atgctttagt
    56041 gaaagatttt cttcttaatg tttatttctc cattcattgg gagtacatgt tgaatacttc
    56101 ttgaataatt tgaataaaga agtcgtttgt ttatatccga tacgattagc gatttcttcc
    56161 aacgaaaaac tcgtttcttg aatcagttcc aacgcaaagt cgaatttttt ctttagaacc
    56221 agttcttgaa aggaagcatc aaaatacatt ttcaatttat tactaagata gaccggattg
    56281 tagccaaaat gagctgctgt ttcttttaac gtacaagaag taaaatgctt atcgatataa
    56341 tctaatactc taagcatctc attttctgcg catccatctg ctgatgaaaa gctttgttgt
    56401 tctaataaat gagtcaattc gatcagtaat agtgatgtat agagatttaa cgaacggatc
    56461 ttatgactgt atgtatcaaa ataaatcaac agcatatttt caatcaaatg cgtcaaacga
    56521 cttgtttttc cgccgcgaaa atgaatgaat tgtgtatgtt cccctttttc ttttgatgca
    56581 tttatcaaaa aagaagaaag tagattattt gtgcttgcca aatgattcaa tatctctgta
    56641 gataaggttt cttcttttaa taagatgttg attaaaatat cctcttgccc tgtgtattcg
    56701 attctttgag tagcttcttt atcaatgatc accacgtcac tttcttccat ctctatcctt
    56761 tctccattaa taatttgtaa acatgtaccg ctatacatat aattgatttc aacaaaatca
    56821 tgtgtatgct cgggaacttc cgcatatcga cggtgtttac tgatatgaat atttccctgt
    56881 tgaaatagat cccctcggtg gatttttggc ggtgtctgaa gatggtaagc taagtttaaa
    56941 tcattgatat tcattccttg ttcacgctgc agagtttcga ttgattcttc gttacgcaaa
    57001 aaaaatgtct aattcagcta gttccaattg actccctcct ttttgtaagc ggtttctttc
    57061 gcttattagt tcacgctgta ctgctattat atgcaaagct atttttggtt tcataaagac
    57121 tttctatggt tgggacatga acttcctgtg attccgctat tgagagaata accacttcat
    57181 actatatttt tgataagcgc ttacaaaaat gaaaggaaca tgcttatgaa taaaaagatg
    57241 atgaaatttt ctctgctgtc agtctctctt attttgactt ctgctggtgc agtcacgtct
    57301 gcaattccca gtatgtcagc tacttatgcc acgcgttctt tatctgctgt tgaattatta
    57361 acgactgctc cctcttttat ggtcactatt tttgttttga ttagtagttt tattgctagg
    57421 aaaatcggac aaaaacaaac tgtgatcatc ggattagtga ttgcatcgat ttgtggtatc
    57481 ctccccctct tctcacaaag ttacagtatt gtcttagctt caagagctgg actaggtatt
    57541 ggctttggat tgatcaattc tttggcagtc agcatgattg ctgcctttta tcacggagat
    57601 gaacgagcct ctatgattgg ttttcaaagt gcatttcaag gatttggtgc agctttaatg
    57661 acatttgttg caggacagct agtacagttc ggttggcaat ataccttttt agtctacgcc
    57721 atcagcttac ctattcttgt cttgtttgtt ttgtttgttc ctacacctga aacaactgaa
    57781 cttcaatcgc atggcacagc cccaacaaga caagtaatca aaacgaatcc taaaatgatt
    57841 agttatggta tttttctctt gctggtcatc gtggtatata atgctgtatc gattaaatta
    57901 gccacagtcc tgacgactta ttctctagga aacgaaacta atgcagcgaa tatcttaaca
    57961 atcatgcaat tggcaagtat ggctgccgga gtttgttttg gctggcttca gacaaaagca
    58021 catcgctata tgcttacact ttctttacta ctgatgggaa tcggctttgt attgattgcc
    58081 actgtgataa acctgtatgt gattgggttt ggtgcgatcc taacaggaat ttctttttcg
    58141 ttattcattc cttacttatt caaccaagta agtatccaag cgcccaagta cgcggagagt
    58201 ttcaatactt caatcttatt ggttggcgct aatttaggta ctttcattgc accatatggt
    58261 ttgctctttc tctcttttgc ttctcttggg agtaaaacac ttgctgtttt cgtcaatggc
    58321 agtatcttgc tgttgattag cgcgcttatt agccttctag tagtacacag gacgaataga
    58381 aagacagtga taagagtaga tgaagaaata aaagaacaat tgacaacaaa ataaatttga
    58441 tggaggtcgt gtaaaatgac aaatgaaaaa cataaagata gatggctttg gtatcctgga
    58501 gactttgaga tccgccatgg actgctacaa aatttccaac gggaagaacg aggttttgac
    58561 tggccagcgt actggtacat ggatgattgc catcggaacg tcaagtttaa gagatattac
    58621 tttcttgatc aaccttccat gtttaaagtg acgatccaag gagttgggta tgtagaaatt
    58681 aatggacaaa aacatccatg tggaaaatgg ctgacttgtc cagctggtaa agcaaaaatt
    58741 cgtatctttg taggacatac atcagggcta ccagcgatgt ttatcgaggg agacgaagta
    58801 aaatcagata ttggctggac agcaagtaat tttattgaag aatatccagc tggctggagt
    58861 ccgctttatg tcgatatcgc aaaagatcca aatcaaatct attatcaaaa agaatgcgtc
    58921 catccagtca ctgaaacgga gattggcgga ggaatcttat tcgattttgg gcgagcagtg
    58981 aatggctgtg tagaactaat atctctggat gaccatatcg aatctatcac tctatgttat
    59041 ggagagtccg atgtagaagc tatggatatc gattattgct actataagca aataggaatg
    59101 acaagaaaaa tgaaagctcg taaacgcgct tttcgctatt tgtttatacc agaggtttct
    59161 actggaatgg ttcaacttca agtttttcat gaatttttat ctctttcgaa acaagctgct
    59221 ttttcaagcg attcttcttt actgaatcag atctggtcag tatcagaaga agccttcaag
    59281 ttatgtagtg atttgttttt tatcgacggt atcaaacgag atcgctggat ttggtctgga
    59341 gatgcgtatc aatcattcat gatcaatcaa tatctttttt ttgacgagga aatcaataaa
    59401 cgaacgatat tagcattaag aggacaaaat gaaatcaagc agcatatgaa tactatcgtt
    59461 gactattcga tcttgtggtt gattggtatc gaaaaccact atatgatgac gaaagactat
    59521 gaatttttac gcatcgtcta ccctaagatg caatcattaa tgagatactt gatggagcaa
    59581 acgaatgaat tagggtttat ttatgggaga gcaaaagatt ggatatttat tgattggtct
    59641 gaaatggata aagaaggaac gcttgctgca gaacaagtct tattactgaa agcctatcat
    59701 tccatgatca cttgcggaga tatcttagct gaaacatatg gagagaacga aaattatcat
    59761 actcatctct atcaagaaaa atatcaacta ttgaaacaaa acctatttga ctacttttgg
    59821 gatgaagaac ttggcgcttt tatcgattgc tatgaatctg ggagaagaaa tgtgacgcgt
    59881 catgcgaata ttttcgctgt tttattcgat tttgctaaca aagaccaaca gcaatcgatc
    59941 ttaaagaatg tcttattgaa tgacactatc acacagatta ctactccata tttcaaattc
    60001 tatgaacaag atgctttatg caaactcgga cagacgaata tcgtctacca aacgattctt
    60061 gattattggg gagggatgtt atctcatgat gcggtaactt tctgggaaga atacgaccct
    60121 actcaaacag gaaatgaaaa gtacagtatg tatggagatc cttatggaaa aagcttatgc
    60181 catgcatggg gagcaagccc aatctatctt ttaggacgct attttatcgg tttgtatcca
    60241 acaaagccag gttatgaaga atttatgata caacctaatc tagattcatt tgaaacactg
    60301 aattgtacgc tacctatcaa agatggaaag atttcgcttg aaaaaacaaa agataagatc
    60361 gtgattactt ctgatagaac aggcggaaca gtgaaaatga atggtaaaag ttatcctctt
    60421 ctagcaaaac agccacttgt cttaacaagc tattcaaaaa taaatgcata aaacaaaaaa
    60481 ctgcttgcga catatcagaa gactttaaat cttcttctga tattgtccaa gcaattttta
    60541 ttttgaagtt tgcttaattc tgttctttag aaacaatgtt tgttcttttt ccggcaaaaa
    60601 gaaagctaga taatacgaca agtaaaaaga aagcgatgat tccaactgtc aaggctattt
    60661 gaccgtattc tcctagtttc ccaagtacaa gtcctggtac tagataatca gtatcggcaa
    60721 atgtagaaga agcgatttta agatctccta atactggaag gataaataat ggcagccaac
    60781 taatcaataa gctattgaaa gcagaaccta ataccgcccc tcttctaccg ccagtcgagt
    60841 tgccgaagac accagaagca gctccgcaga agaaatgtcc tactacccca ggaatgataa
    60901 ctgtagtttg caagcctatc atagcaaaca tggatagtgt tcctacaaag aagctaacaa
    60961 aaaagccaat taatacagca ttaggtgcat atggaaaaac gataggaaca tctaatgctg
    61021 gtttagaatc gggtacgagt tttttagcaa tcccttggaa tgctggaacg atttctgcca
    61081 acaccattcg aaccccttga aggacgataa caaacccagc tgtgaatgtt ccagcttgga
    61141 tcagtgcaaa gattacagcg ttgtctccat tgcttagttc tctttcaaca aatccaggac
    61201 cagctaacaa agcaagtgcg acatatacga tcgacatcaa gagcatgatc gatacagtag
    61261 aatctcgcaa aaagctgaaa cttttaggaa tctttatgtc ctctgtagaa cggtctttgt
    61321 ttccaaaaaa ctttccgatc catccactag aagcataacc gatatttccg gtatgcccta
    61381 acgcaacttg attgtttcca gtgatgttct tcataaacgg ttgacaaaga gcaggcgtaa
    61441 tagttaatag tgttccttcg aagaccccgc ctaataagat catccacaaa tttgcagtaa
    61501 accccgcgct tactaaaatg actgctgtta gacaagaaac ataaagcatt gcttgacctg
    61561 taagaaaaat gaatttatat cttgtgaatc tagcaagtaa aatattcacg atcatcccta
    61621 ctagcatgat caaagcagta ggtgtaccgt attgttgcaa agcaatcgcc actactgctt
    61681 cattgctagg aactactcct tgagtatgca gagctgtttg gaacatcgaa gcaaaagggg
    61741 ttagtgaatt actgattaaa ccagcaccgc cgatcagtac taggaatccg acaaatgttt
    61801 tgatagttcc ttggatcact tctgaaaatt tcttcttctg aagaacgaga ccaagcagtg
    61861 caatcaaaga aacgaggatg gagggctgac tgacgattga cacaaacaaa ttcaacaatt
    61921 tatccattga tagttcctcc cttgaatcat ttttattctt ttgacaagat cttcgttact
    61981 ttttctttta cttcttcgct gtcaatgatc gattgcaaag gtacgagctt ttcatcaggt
    62041 atagaagtta gcgtatcttt caaagaatgc tcaatgaaga aataatcagc catatcgcta
    62101 gatacacttc caacatccgt atgacttact tcaaactcgt ccacatctgc tccaagatct
    62161 gcaagtgctt gtttgatgtt catctctacc ataaaacttg ttcctaatcc actttgacat
    62221 actgcaaaaa ttttcatatc attcgtctcc ttttcttatg atcgctaata tttcttcttt
    62281 tgttgatgcg tgtaataaat cttgaagatt ttgtttatta gaaagtagct ttgttaaatt
    62341 tgccaaagcc cgaagatgtg cctcattatc gatagcagct aaacagatga acaaactaat
    62401 gggatgggct ggattatcaa gcagatccac aggttgttga agtttcagca aagacatccc
    62461 tacttcgttt acgccatctt ctggacgagc atgaggtaat gcgatgtgat cgccaatatg
    62521 gataaacgca ccatactgct ccacgcgctc gatcatagct ttgatataac tttcttctat
    62581 tttattttct gataatagcg gctgtgcggc aaatgtgatt gcttcttgcc agttttttgg
    62641 ttgatccgat aattggatca tttcttctgt taataattca gatagcatag gacgttgatc
    62701 ctccttcttt tctaaaagtt tgttcatttt acgatttaat acttgataga gcttttcttc
    62761 gtctacgcct tcttttaatt cgatataagg cagtagcgcc gacaacaatt cttgggcaga
    62821 aggcattgaa aatcctggta tcagccaatc tctttgtact tgattgatca gctgattttt
    62881 ttccaagtca gtcatgattg gatgcagaac atagagagac tttttcttat cagtaagtgt
    62941 gagaggtaca gtagaaaaaa taatatcgta agaagtttcg tcaacctcgt gtaagcggtc
    63001 agctgaatta gtctctgtaa aaaagatcat cgggaataat tgctgaagtt cactttttaa
    63061 gatcaatgaa gagctaatgc cacttggaca gacgatcacg gctcttattt ttctatttct
    63121 ttcttctgcg tccgcttttc tgatctctcc gccaaatagt atagtgaaaa aaccaatctc
    63181 ttcttcaggg atgctcttcc ctattcgtct ttctaacgga agtaatgctt ttcttgtcat
    63241 ttcgtagatg gaagcatatt gatgcttaat ttgatcgatc atcacattag gtaaataaaa
    63301 agaataggaa atacgaaaat aggcagggac caagtgataa aagacattca tcaataattc
    63361 acgaaacgaa gcaaattcta cggccatcag gaattccatg cgatggatga cttctgaaga
    63421 acattccagc aaaaaatcaa gtgcaggctc ctggacatct ccttcaacga tcgatgctaa
    63481 gcaaagagag aaaaatataa tctccgaatt ttgatgttcg gaatagccaa ggccagtcaa
    63541 tacttgtcga ctaatgtcag tgactgcaag agaagccaac agttcagcat cttttaccag
    63601 ttccatggat gtttttccaa ttcgaatggt tgtcaaactc aaataataag ctaattcatc
    63661 cattcgattt ggtgcgaatc gaagatctga tagtgaaatc gttttatcta gcaaatctct
    63721 aactttggcg taataatcaa tggaaagggt tgcggttaaa tacgtgagcc caaatcttcc
    63781 agcaagtgac tgcatcaaac gagcagtgcg attttttgct tctcttctta atattgcttc
    63841 tgaccctagc agcctaaaac cagttttcct agtatacgac agctgacaat cttccacttc
    63901 taattcattt cttatctttt tgatatctga caggactgtc cctttagaaa cctgaagaaa
    63961 atcttgaaaa tgaaaaacag aaacagacgt tgcagtcgaa taaaccatca aataaagaag
    64021 atctcttcgc tcttgttcgg tatacacagc ttcaaatcga gtaatatcca aaaatttttc
    64081 tgcccagtcg attgctcgga atttctttat acggatacgg gtatcatcca catattcggc
    64141 aaaaggagtt aattgtttga tcgcctcgct tgtatttact tcgatgatct ctgctgcttt
    64201 ttctagactg atttctcttg gtgttttcag gagataacta attttgtagt tcacagcgct
    64261 gttcacctcc tacttatatt ctagtatggt agcgctttta aatcgagggg tgtttggtac
    64321 aacttacagt tggatttttt ttacgactgt accgaacgat ttctatcgaa tggatacaga
    64381 aaaaaagcag gtgaccaaat gtctcctgct tttatgaatt gatttcgtcc gatcaatgat
    64441 gatcatgttt attttttgag gtcagttctt caaatttttc tttgacatct tcaaccgctt
    64501 tttcgatatc tttttcaaag tgattgggct cattaggctt tgtttcctct tctacagaat
    64561 ttgaaccaga taaagctgtt tctttatttt ctgtttcacc agagtttgtg ccagctaatg
    64621 attcttcttt gtcttctgtt tcaccagaat tagttcctgc taaagccact tcttcttctg
    64681 ttttaggggc cattgaacct cttaatcgtt tgtgtttttc accgtcataa tttgtcatgt
    64741 tggttcctcc tagtagttct atactttttc atcgtacaat aagacagttt atgcgtcaaa
    64801 aaacaaaccc gattcttaca aatatgtcat atttttcctt ctgattcttg aatgcttttg
    64861 attccttctt ccaaaatttt catttgtaag attgtttctt catctttgat gactttttct
    64921 gcgccattaa ctaatgtctc gtaaatccct tcgtagacac ggctgtaatc accaacctcg
    64981 gaaattactt tttcttcatg ataactccct tgttcatcta tataggtcaa tacaccataa
    65041 tgttcgggca aatcgatacc aaagtcttca tgatcaggca tgtaaaacat tttcagatgt
    65101 tcttcttgtc gatcttttgt ttgtttcacg aacatccctt tttttccgta aacgacgaaa
    65161 cttggacgtt ctttgatgcg gaaataactg gattttatcg aaattttcat atttccatag
    65221 aaaaaatcca gatcgaagta atcattcatt cgtcctttcc ctaaaagttg acgtacatca
    65281 tagctgatat catctggttc gccgaaataa gaaagaactt gatctactgt atgacaagca
    65341 tgtccatata aatagctgtt cgcagtagaa aattcatgcg tattttctgg tacttccggt
    65401 ctgaaataat cataatgcat ttctacttct aaaatatccc ctaattttcc gctttcgatt
    65461 actttttgga ctgtcaagaa gtcagaatcg aaccgtctgt tttggtaaca ttggataaat
    65521 agaccttttt cttttgctaa tgcaaataac tcttctgctt cctttgacgt ttctgcaaaa
    65581 ggtttctcta ctagtacatt cttaccatgt tcgagtgcca tttttccata ttcataatga
    65641 aattgactag gtgtagtgat gacaactaat tggatctcag gatcatccca aagatcagac
    65701 agctgatcag tatattcgat gtcttcgaat gttttccaca ctggttcaga tggggaaaaa
    65761 atttttttga tattgatttt gttcgtaagt tttcttgaaa aaggcaaatg atagcgattt
    65821 gtactctttc cattgccaag ataagcgatt gtaagcatga gtggtgcctc cttttattga
    65881 tgttttcagt atatcctaga aaagcgcaga aagtatttat aatagctatt ctggtaaaaa
    65941 agttcagaaa aacacttgtt atcattcggt ttggtgaaat taatgttcaa agtaaggata
    66001 aaaatcaaaa agatagtagc ggaaccaagt gtttatcagt aagatagaat ttggaggtca
    66061 gatcatgctt attgaagaaa aactaacaaa acaagagttg tttaccacga cagagaaacg
    66121 gattgtcgat tacatccgta ggaatatcga agctgcagtc tacatgacga ttgaagaatt
    66181 agcaaaagct acgtatactt cccattcagc tatcattcgt ttatgcaaaa aaatgggatt
    66241 cagcggattt aaagaattcc gtttcgagct ggggcgggaa gttcatcaat tgattgctca
    66301 gttcaatcaa acgattgatc ctaattttcc tttttcaagt gaagatagca ctcagcaaat
    66361 tgcaaagaaa atggcggatt tatcgatcca gtcaataaaa aaagcacaat tgcagattga
    66421 acatgaagat ttagatcaaa tcggtaaagt attgaacaag gcacagcgaa tctttctttt
    66481 tgcaaaaggt gattctcaaa tcacggcaag gaaattccag aataaaatgg tcaagttgaa
    66541 taagtttttg atcatggctg aagaatacag tgattcttct tggaatgcag ccaatttgac
    66601 tagcgaggat tgcgccattt ttattagcta cagcggtcgc atccatcatt atgaacgaat
    66661 aatgacctat ttgaagcatg tagaggcccc taccttgctg attaccggaa atcagcattc
    66721 agaaatggcc aaacaggctg atatgtgtct tgttatatcg caggaggaat atgattttgc
    66781 taaagtagcg actttctcct ctcaaattgc cttcgattac gtattaaata cgttattctc
    66841 agtgatatat tctcaaaatt ttgaagagaa tatccgcaac ttgaaaaaca aacaagaatt
    66901 gatgcaaaaa ggccttctta aagaatatcg gaaaacttaa gaaaagatcc tcatttagta
    66961 ataagaaaga tagtgtttga taggctctct ttgatatcat cataaaaaca ggctgaagtt
    67021 ttctttttca aacctcagcc tgtttgttta cggaacgatt cgaatagata tattatagaa
    67081 atgaggaata gctaacttct gtttcttatt ttgaacgatt gacgaaaaag atatataaga
    67141 aggaggaaaa tcagtataca tgcagctaac aatccataca ggaaaaatcg tttgttctcg
    67201 gatactttct gtacttcttt ttgaacttct tttgcatgat aaggaacccg gtgccctctg
    67261 acaagtaagc gatgactgtt gaccatataa ggtgtacaag ttaggagtgt cacgaaatcc
    67321 tgcccttttt cgattctcag atcttttgta tcagtcggtt ctaccgtctt gatctgatcg
    67381 acttgataag cgagtgtccg tccattgatt tcgatataga aagaatcacc taaagtcagt
    67441 ttcggtaagt ctgtgaacaa ctttgcttga ggcagtcctc gatggctgga aataacggca
    67501 tgtgtatttt ctccccccgt aggataagac gttccttcca ataatgaggc acctttttct
    67561 agcagcacag tattcgtttc atcaaaaatt ggcaaatgga cattgatctc agggatcgtc
    67621 aaaataccga tcgtatgttt ctcaaaatag gttctatcct ttttcgcagg ttcttttttc
    67681 ttagtaaagg gatctgcacc tggatttcca ccttcttttg ctaattgctg gtttttcttt
    67741 gtcatgtttt cttggatttt agccatgact tcttcatttt ctttgaccgc ttgttgctga
    67801 taatgagaga tgatctgctg atccagataa ttattcagcg cgtcgctaac aaatggataa
    67861 gctagtatcc ctattcctga gagaagcaag aaaagcatca gcagatctag aagtttcctt
    67921 ttctttggtt tcatttgtgt tctccttgtg atgactgttt gataataaaa gaaaaaggag
    67981 catttgcgta atccgctgct cctttttcca attcttgcaa aaagactaaa tctgactgtg
    68041 cttgcgtcta gcaaagtaca gtccagcaat cagtagaagg actactcctg caccgatata
    68101 cacatagatt cccttaccgc ctgttgaagg aagtgtacct ttgtgtttat ttggtatttt
    68161 ttcaggagaa atcaactgtc ctgctgttac ataagattgt tcatcgatag taaatgctac
    68221 acggtttgtt aatggaacat atttttctgg cgctttcgtt tcttccagat agtacgtgcc
    68281 atattttaga cctgtcacat cgattaaacc gttacttgtg gttgtaaaaa ccgttgctga
    68341 ttctttcgcc gatacccagc tgacggcttt tgtggatggg tcgatcgata aatatttcgc
    68401 tgtgtcacta tcttgatcac gaacgacgaa ttctgctcca gcaagtgttt ggtctgatgt
    68461 aacgtcacca tctactttaa caaatcgttt gcccccagta acgacatcga ctgacggtgg
    68521 tgtttgatca tttgtatgcc cgttatcgac atttgcttgg ttagaaaatc ctttggttgg
    68581 atctgctttt tcgttcaaat gcatatagta aacgaatttc aatgtaccgc caggagttaa
    68641 tgaaggaata taattcggat caactgaaac cgtgaatccg tctgtttgct cagtgacaga
    68701 ataatttact gggtcgattt ctttatttcc atcatataag gcataagcat acgttcctga
    68761 agaatcatta tcaaatgtta aagcagcgtc atgagtatcg ataagtttga atgttgtgta
    68821 cttgttttgc gttccttctt tatcagcgat acctaatggg atattgacag aaatctcata
    68881 ttgaattttt tcaccgattg cgacatcttt tccattcaat tgaggtgttg ttttatcaac
    68941 tttagacgta tcatttttga tggtcttttc tactggtgtt tggctatttg ccaggatctc
    69001 aaaaggcgtg attgtttgct tttcaatcat ttccgcatta tcaggagctt ttacttcttc
    69061 taaattatat tttccaactt ctaaatgatt aacgatcaac tggccttttt caatagatgc
    69121 ctcggcaaag tcattgttgc cgatgtcata agaatgacca gtaatgaaat gtttggcttt
    69181 ggtttgatca gttgtccaag tgtacaatcc atctgtgaca ctttggatgt attttttgac
    69241 gcttggtgtt ccttcttctt tagaaataat aaattctgct ccatttagtg cttcgttttc
    69301 ggcagtaccg atttttgtaa ctttcaacgt tccatcatta ccgactgtat ttttagggta
    69361 gagatggata gtatctagtt cttctgtccc gtatttataa gagccatctg cttgtttgat
    69421 catctcatat acagggaaag ctaaaaccat gtttgcggca gctgacactc cgtcttttgg
    69481 ttcttctttg atcgtgtaga ctgcatcttt cccattttgt tttttaggta aagataaagt
    69541 gacatttcca tcagcatctg tcgttcctga agcaactggt gtaccaggag tcaaagactg
    69601 gactgcttgt tttgctgcat ccacggacgc tcctttatct cgttgcgcat aaaattcttg
    69661 agtgacgtta taaactgaaa atgaaatatc ggctaatcct tggtattgat cgaattcgct
    69721 catttctttc ccgctgtttt ggattaaagg atcgggtaaa tcagtcattt tctttttatg
    69781 aaggatgact tgagctgctg tctcctctgc aaacactttt tttgcgccga agctgtttgt
    69841 gagtaatggt aaaataagga aaaggactcc taacataacg tttatttttt tatgattttt
    69901 cattttttca ctcctgttct ttttttgata aacaaccaga ttgcaaagct tgcgaatacc
    69961 ataccaacaa cgcctaaaag tgtatttttc gtttcgtttg tttttggaag aaatggacgg
    70021 ctattttctt ttttattgac tactttcaat atactgtccg cctctttatc ctcagaaaga
    70081 aaagttgaaa tggtaaacgg aatttctgtt tcacttaaaa tataaccaga aggtgcttcg
    70141 atctctttta atcgataact tcctgttttc aacccggaaa ttgaaaaagc gccattttta
    70201 tcggaaatca ggtgaagcgc atctgattcg ttctttgtcc acttgtatcc gttggctgtt
    70261 tgctggaggt aatttcctgc tttgttttta ataaggaata ctgcgccagc taatcgttta
    70321 tctggtttat ttgtctcgat tttgacaaat tgcttactcc ctgtatatac agagactttc
    70381 tttgtactga caccaaaacc aaaatccagt gttccgttgt tgttgatttc cttgttcgcc
    70441 tgtgctgtac tgcttagctg catctgataa gaaatggtca gtttttttcc agcatatttt
    70501 tccaacgaag ggatcgaaaa taaaacatca aatccgtgag gtttcttgtg tatgacaaag
    70561 ccttctgtca gcttttctcc attcgatgaa atcgttaaac tattaggtaa aaacgttaat
    70621 gcttcatccg cactatctga caattcgaac ttttgatagt caaggatatt tacaggaatt
    70681 gtcgtatgca aggaataagt gattttttca ccaatcgtaa aatcatttct aggctcttct
    70741 aataccttct caaaaggtgg atcagggtag tcgttttctt catttttagg atacaaatgg
    70801 atagttgaaa gtttttgatt gttttgtcca tgtacaggaa gaacaactac catattctct
    70861 gccttttctt tgacgacttc tggtactttg gattcaatga aaagatacgc tttatctctt
    70921 ttttcttgat ctttagcggc cagagaaaaa gaagcgatac catcttcgtt gttaagagtt
    70981 gtagttgttg cttctgcggt aaacataccg gaagacgcac cgttttttgc gatctctgct
    71041 tgtgcttctt ctaccgtttt gcccttttcc cgtagatggt aaaaagaatc tgtgacatca
    71101 taaacttgga atgtgacacc atttaatcct cgatacgttt gtaataaagc tttttcttgt
    71161 ccgtcatttg gatgattttt cggcagttgc ccgtttggga aaagcaattt gtgtaaagta
    71221 atctgaacca tttctgtttc tgtttctgtt gctacctgag taaaagctgg tttaaatagt
    71281 aacaagaaga gacacatact aagccaacca agttttttca tatttagctg ccccctcttt
    71341 cttttttcaa aaaaagatac caaatagaaa acgcacaacc aatcatccct actagataga
    71401 ttcctaaacg gccaattcct cccgtttcag gtaatggtac ttttgcctga ttcgtgatgt
    71461 ctaaagaaat ctggttgttt ttggctcctg gtgacagaac agattcatga tcttgtccat
    71521 ccacttgaat tgacccatct tcgtgtataa ctatagtaac tggctctttt agaccttggt
    71581 atccttctgg tgtaaaagtt tcggtcagcg tataagttcc aggttttaaa ttttcgaata
    71641 gaaaggtatc tgtttcttgt ccatcttttg gcgattctaa gtccatttct ggtccctgca
    71701 gtctgaattt tgctcctttt aatgtctgtc cgttgtcttc ttttttgtgt accgttagat
    71761 caaacgcttt tagtcgattt tgatgagtga gcgtccatac attatttgtc atctcggtat
    71821 tttttcccgt gtaaatgatt tttccatatt tatcgatttg gaattcataa tttcccggcg
    71881 aagtatcata tccggcagga ccagctgttt caacgactcg atatttacca ggtgtgagtg
    71941 aatcaaaact ggcaagacct ttttcattcg ttgtttggct gtcaattggc tgataagtac
    72001 catttgcttc ttttttctgt agggaaaatg ctgctccttt aagatttatc tctttgtccg
    72061 tcccttgcat cgcatactta cgtactgcta caggaacttc aacaaaagga ttttcaatag
    72121 tcaactggat cgtatcatcg gaagtagtga ctgtttttcc atcaatggtt accgtaccat
    72181 cagaagcgat cttgatttcc caagtagtct ttttgcttaa tccatgccct tctggagctt
    72241 ttgtttctgt cagcgtatag gtcatttctt tttgcaattt gacattttct ggaagagaat
    72301 aggttccgtc gccatgatct gttagtaaag tatcaataga atctcctgtt aatttgaaaa
    72361 cagcgccaat aagatccttt tcacctgtag aggaattttt cgttattttc aagtttaacg
    72421 gtgtgaattg tttagtattt ttccatgtca ttgcatctat ttgttgagaa tcgtatcctg
    72481 gtacaggtaa ttctttgatt gtttggtaac tgaatgcttg accttgatta ttgtattgag
    72541 gtagtgataa tatctcttga taactttctc cgctatttgc agataatttg tcaatgtctg
    72601 cacgttccca cgtatttgtt gtatctttag ctggtttaat gattcgaata tatccgtttt
    72661 tccaagctgc agcatttgtc gtatgttccc gttgaatctc aaaagtaact tgatctggac
    72721 gatcagctag attgttgtca aattcttccc ataacttttt gatgtgaaga ctgactccgg
    72781 gagctttagc agatggtata ccgaattcag ctaattcatt ggtatcaatg tttggctgga
    72841 aagttgtcct gccgttcatt tgataccaga aatttggatg gaagtcctca ttttctgttt
    72901 ggattctcac ttggtaatgg atttggattt cttggtcttt tcctaaatag atctgattgc
    72961 tcttgatggt atttccttct atggaaatag atggagtgac cgttgtagga cttgtcccca
    73021 cactcttgac tgaaagtgta ccaggctgat aaatgaatgg ttctgcgatt ggatcattta
    73081 tttgtccatt gcttacagtt gctgagatct gtacagcttt tttggctagg tattcagaga
    73141 tatctgttgc atgatcagct gattcatagt aaagatcgcc tttttcatct gatgaaacca
    73201 tttgtcgcat acgagactct acttctgctt ttgagagacc agctgccgga tcgctttgaa
    73261 gttggatgcc aagaccatgt atttcgattc ctcgttcttt gagtgccatc gcttctccga
    73321 tggttgcgat aaacgtactg tcgattcttc tggatagatt gttttggtca ggtgcatcat
    73381 agattcttga gattagagaa gtatttcccg gccgatcttg cgtattagaa aactgagttc
    73441 cgtaataatt gctgcttgat tgtgcgtgta cccgctgtac tttataggaa aatgttggta
    73501 caccatccgt cagcaaaacg atcacttttt tatgtccatt tggaacggat agcatgcttc
    73561 ctgcatctct tagtgctttt tgtgtaaaag taccaccatt tgtccgtgaa ggtgtaatgg
    73621 attttacttg atttttcact gaatcaaatg aacccatctg tactgcaccg ttactgtagc
    73681 tatatccttc gcttgagtag ccgacatatc ccatattgat tttgtctgtg ataccgctat
    73741 ctgctaaagt atcgacaaaa cgatcgacac caatctttac ttcaccgatc cgattattgt
    73801 cgttcatact tcctgaccag tctacgacca atacgagatc aagaggcgtg atatcttttt
    73861 gtacatttcc acgagcattc aaataaacat caaacaatcc gggtgtactt gtttcctttg
    73921 catactttcg tatcgcatag tctgcttgat taccggttcc tccatattcg atatacgaat
    73981 gggtccgatc attaggatct ccattccaag aatttatacc atcccattga tttgttccgt
    74041 ttttattgcc ttgatgatca agaacatttg tatttcctgt aggttgccaa ctggctttag
    74101 gataagtgcc ctgatcatct gttgtatatt ctgtaggaat cgtttgatta gatggcacac
    74161 tcacagctgg caaggataat gtaggcaatg taaatgggct gacagaattt tcgttttctt
    74221 ttgatgattc ttcttctatt tcgtctggct ttggtactga cacatctgta tctttcttat
    74281 tttctgtctc atcaataatg tcatattctt tattatcgac gatcagcttg tttgcctggc
    74341 tgtggtaaag tgctggatcg attggaaaac tcagatcgat agtttgttta gtgtttgatg
    74401 agatttccaa cgtaattttc ccgctattta cctgataagc atagccatct ccttcttttc
    74461 tttccacatc acttaagtaa ttttcgacta gaccaatttc tttttctatc ttttcagcac
    74521 tggtgttatt cgcatttagc ttgatgttca gtgctttttt tccagactga tccgaaatga
    74581 cttcagctga aattgtttct aaaactgttg gatcatctgt agtgtctgct attgcagata
    74641 atggcacaaa gtttgataaa ctcaatatta tcagcatgaa aataactttc agtttcttcc
    74701 ctgtggtcgt catttttccc ctcctttttc tttttttctg ttttgacgtg aatagtgaaa
    74761 attattaaaa caaacaggcg ttacataatt ttatttattt taatatgata attattatac
    74821 gaagggcgct aaagggaatc acaccaattt tcataccaat tggtttcatt tatgaagttg
    74881 attccttcga ttaatgagcg tttcattgaa agaaaagggt tgagggagaa aaacatatga
    74941 tcaggttgtt ggaaggaaac tatagaaaga tggaaaaaat cattcgaatt ctgtccattt
    75001 ctgacaagtg gatgactaaa aaagaacttg ccaaacgaat tggtagttca gaatctacat
    75061 ttgtaaggta tatcgaagag atcaaaatcc gctggggaac ggatgtaacg attcaatctt
    75121 cacaaaaatt ggggtatcgt cttgaacatt tgaatgtacc agcttattta aatgttctga
    75181 tggaaatgtc gcaaagttct acaaacagtc gtttattaaa tgagattatt gtcaatccgg
    75241 gtaaaacgat tgagtattat tgtgaaaaaa tttccatcag ccggtcttct tttacacgta
    75301 agttgaaaca atgcaatcaa attttagaaa gctatttatt aaaagtgatc gttgaccaag
    75361 gttttcagtt aactagcctt aaaagtgagc tccagttgcg tatctttgtt acctttttct
    75421 ttttaagtta ctacggaaga cgcacgttgc catttccaat cgaaaaaaag aagatcaaag
    75481 aaattatggt caagaataat tgtcagatga atttgtttca ggaagaaagc ccatatgagc
    75541 aatctttctt tattatgtat tttgttgtgc atctcatgag agaatcccaa ggaactccat
    75601 ttgaatggaa agctggagac tcttatgtac agttgataaa tatcgataaa aaagacttct
    75661 taatcttgaa aaatctctta ggaaaattga agtacagcag ctatataaaa gtagtgaact
    75721 attttgtcaa tacgattttt tcagaatttt cggagaaaga aaaaaatgaa atcagtgagc
    75781 agatacgaaa aaaaatgtcc tactgttcgt tttcatccgc tgattgcaaa gaaaaaaacg
    75841 aagaatatgt tgttcaatta ttaacgcaaa tttatttttt ctcccgtatt ttcccatacg
    75901 atacgactca tttgacgatc cgtgcaactt actttgcaca gcagatgcaa caccgccaga
    75961 tacgtctata tgctggattt cgtgcagaac ttcaatttta ttccactcta ctgaatcaga
    76021 atctgattta ttatttatct agctatatct tttggatgct tatcggtaat cctgaaattc
    76081 accattatac ttctgataaa aaaatactga ttatcagcga cttaagtctt cgacatagtc
    76141 aatatattga agaatatatc agtgatattc ttgctgtgca taaaattcac tctgagacaa
    76201 ctgctattac tgaagatcag ctgtcgaaat acaatcttct cgagtatgat ctgatcgtta
    76261 cgaatcaacc cattttgaat gcccatgttc ctcatatatt gattgatgat agtgtgtcat
    76321 tcgcaaatga agaagaactc atccgcttat tcgagttatg aataatcaaa aaacagctat
    76381 gtataaacca ccctgataaa aatttcagga caaatacaca tagctgtttc tttttattgt
    76441 attccttcag caatccaacg agatttatag cgaaagtctt ttaacaactc ttcttttgtc
    76501 caattatttg caatgagatc cgtcaaagtg ttcattacga gggtcatgga aaagtatagg
    76561 aaactaactg cttcttccga cgattgaatg gagaaatgat tgattcttaa taattccagc
    76621 catttttcgt tcattggaga atcagccgat agttctctac gtttggttgt ctgctgatca
    76681 tttcgctggg ttaacaagta taacctttgc caatagatac tttcgtgatc taagtcgcag
    76741 caccactcta aatatttttc gatccccaaa aagaaatgtt gtttgttttc gctaatgaat
    76801 tttaagatat cttgatggac tatgtgagaa ctgcttttga tcgtatacaa ataagcatct
    76861 tccaaatctt gaaaatattt ataaaaagca ccgcgcgaca tccccatctt gtcaacgatt
    76921 tctgagactt tgacttggct gatgtgctgt gagcaaaata tctccagtaa taggtcatcg
    76981 attctctgtt tctttgcgtt cgataaattg aaaaaagttt cttttggcat ccacatctct
    77041 cctttatctc tgattatacg tggatagccc ttcattgtca tgaaggattc attccttctt
    77101 tttctcttct tctgtcaagt acccatcttc cattcgataa acgcgatcac tccattgaat
    77161 catacggaca tcatgagtaa ccataatagt cgctttttgt ttctcatgag cttctttaac
    77221 tagtaatttg acgacttcat atgcatgatc tgtatccaaa ctagctgttg gttcgtctgc
    77281 taagatcaag cttggctcat tatataaagc gcgagcaatt gccactctct gtctttctcc
    77341 accagaaagg tctttaggat aactattttt aagtgactgg atatctaaag attgcagcaa
    77401 ggaatcgatt tttgttgaat cttctttttt tgaagtaact tggtcaacta gttcaaattg
    77461 ttcctttacc gttaaaaaag gaatcaaatt agagctttgg agaataaatc ctatttcttt
    77521 aaagcgtaaa tctgctcgtt ttttttctgg aagctcagtt agtgagtgac cgttgatcac
    77581 tatttctccg gaagtcggtg tttgcaatcc tcctgaaatg gtaaggaaag tacttttacc
    77641 tgaaccagaa ggaccaatga tgctgacgaa ttctccttcc tttacataaa aatcaattgc
    77701 tttcaaagct gagacctttg tatgaccaga accaaatgtt ttggtaactt ttttcatcgt
    77761 taatattttt ttcattttta ttaacctcct attgctgtaa ttgggtctac tttggttatc
    77821 gtacgtatcg aaaacaatcc gcctagcatt gcgacaatga tcaaaatccc actatatagc
    77881 aaccattgtg accaaaaaac agcaaaaggc atcgcgcttg gtaaaatcag actagtcaaa
    77941 taagcaaaca aaagagcaag cagaacgcct accaccccaa caataaagga ctgtgctact
    78001 aaagatttag cgatgaagaa atttttgatc ccttgtgctt tcattactcc aaaaatagca
    78061 gtcttctgaa gtgtcatcac gtacatgaaa ataccgacca cggcagcaac cactaagaac
    78121 aaaaagtata tcatcgcatt taaggtcata ttttgggcag aatagccagg aatactttca
    78181 atgaatgtcc caatagttaa taactgtaga cccttcgatg ctttttcttt cgaaatttta
    78241 gctgaatcct ttattacgat cccattgatc ggctgttctt cttcagaagc aaacggctgt
    78301 tgtccatact tcaaagcagt ccacgtatct agggacgcat agatgacggg agaaactgtg
    78361 taataagttt caggaaagat accgacaatc gtcaaaggat cgtcgtaact gccaattttg
    78421 atcgtatctc caattttata gcctgaatca gccagatttt gagagatgat gatttcattc
    78481 tttgttttaa attcatgccc tttcgtgagt tttggtaata aaaatgcttc tttcgttgtg
    78541 ccgaaaactg taacattttc tttgtttttt cctttgattg ctccgctgta caatccgatt
    78601 ggtgctttct ctcctccttc aatgtatttc agatctcctc ttgttaattg tgaagctgca
    78661 aatacttgat ttgcatcctc cgacaaaaca atctcttgtg cgtcccaatc atcgattgct
    78721 tttttaaact cttctgaaag tccattcgct agtcctgaca acatgaatac gacatacgca
    78781 atcaagaaca taataccgac gatcaagcca tatcgtaatt tagaatagcg aatttctttg
    78841 atagctagaa acatttgttt ttcctcccta tgatagattg atagtgacaa aatgtcactt
    78901 gttgaatagt gtaactctaa agtgacaatt tgtcacttgt gttgtttttt tcttatcaat
    78961 aaattgtcta agacatcttt cgataaaacc ttaacatgag tcataaaaaa tttatttaag
    79021 ccttatttat gctttccatg ataaactaaa gtgaccaaat caatgaggtg ggaaaatgat
    79081 taaactatta gtagtagaag acgataaagt attatccaat aatataaaag aaatactcgc
    79141 tgcaatcggc gagataacac aagtttatga tggtagtgaa ggactgttcg agttggaaag
    79201 tggagtttat gatcttgcaa tcttggattt gatgatgccg gaaatgaatg gatatgaagt
    79261 gttagcaaaa atacggaaag aaaaaataca gactccagta ttgattttga ctgcaaaaga
    79321 tagtttggaa gataagattg aaggatttga acgaggtgca gatgattatc taacaaaacc
    79381 tttctatcga gaagaactga tcatgcgcgt gaaagcatta ttgaaacgct cgctaggtct
    79441 ctttgatgaa aatgttctga agtataagga tatcagttgt gatttaaaaa gcaatgaagt
    79501 cttgtataaa gaacaagttt tgcccataca aggaaaagag tttgaattgc tcgtttattt
    79561 tttacaaaat aagggaatca ttttgacaaa agagcagata tttgaccgga tctggggatt
    79621 tgattctgat acgacgatta cagttattga agtttatatg agcaacttac gaaaacatct
    79681 gcgaagtact ggattggata aagaaatcaa aaccttacgt aatgtcggtt atatactgca
    79741 aagggtgtga tgcaatgaat ggagatatta cacgaaagca acgtagtcgc ttttttgcga
    79801 caaatgtagc agcatttgct tttatctttt ttcttttggg attcattact ttacagatat
    79861 taaaccaatc tgcatacaga gaaacagatc tgtcattaga gaaaataaat tttgattctc
    79921 gaacagtaca attagagata caaagatatc agcaaggcga tcccttttta aacccgactc
    79981 aaacggaaca aaacagaaca aacatggaac agccattaat ggaaccatca aatgaccgtt
    80041 ttaacactca agtgatcttg tggtctaaaa atggtgagat attgaataaa gcagctattg
    80101 gtgggcgttt caaccaaatc aaagaattaa aaatggatac gaaaaatctg aataccatcc
    80161 aaaatattga attaaatgaa gacagtggct catctgccct ctctttccgt tcgattacga
    80221 aaaaagcgga gacgaatcaa gatggcgttg cctacatcca aattttagaa aatacaaatc
    80281 aaatcaagaa ttcattagcg acttttcgca cgattatgat tttgtgtatg attgtttttt
    80341 ggattctctc tattggtatc agttattact tgtctagtct aagcatgcgc cctattattc
    80401 tttcttggag aagacaaaaa gaatttgttg agaatgcttc tcatgaattg cgcacaccgt
    80461 taacgattat tcaaaatagt ttggaacatc tttttaccaa gcctaatcat actattattg
    80521 aagaatccga aagcatcgct caagcattgt cagagacgag gcgcctcact ggattgactt
    80581 cggatttgct gacaatcgct cggaatgatt ctgatcagca gctgctttca aagcaaatga
    80641 tcaatacaca agaatatatc gaacaacttg ttaaaccatt tcaagagatg gccatcatag
    80701 atggaaaaga gtttattctc cataacgatg caacattaga agtaccaatt gatgaaaaaa
    80761 aatccatcaa gttttggtta ttttgctgga taatgcatta aaatatacga atccagaaga
    80821 taagatcatt ttggattcat ctgttaacca taaagaatgg ctgattcacg taaagaatac
    80881 tggcccttct atcagtgaag aagatcaagc aaggattttt gatcgatttt atcgggaaga
    80941 tcaatctcgg gcgaaagaga ctggcggata cgggcttggc ttggcaattg caaaacaggt
    81001 aatcgaacag catcatggac agatttctgt atccaattat cttccacaag gagtcgattt
    81061 taaagtccaa ttgccgttga agcctaaaaa ataatgctta tccctaaaaa agggctgtga
    81121 caaaaatctt gtcacagccc ttttttgttg cgaataaacg gtgttccaga agtagcttct
    81181 tcggaaataa gccgaaattt ctttaagctg accacttctg tcacaacctc tttagcttac
    81241 gcttacgatt ttttattgcg gtcttcaatg tgtacatcac cgcttgtgtc aatatccaat
    81301 tcttctcggc taacatcttc tgtcactttt tcaacatctt catgttcttc tttatgaatc
    81361 ccaacttctt cacgaataac tgtatgtttg cttacatcta cttgctcttc tttgattggg
    81421 atagtaatcg tgtcatcttc caatttttcg tctgttccct gtgaagattg atctgttact
    81481 ggtttgcgtt cgatgacgat ttcttcacgt ttgaccggta cttctaccgt ttcagtatcg
    81541 ttgaccacat gtttgtggat gtcgacttct ccagttgtca catcatgtgt atttacatct
    81601 aatttttctt cttttagttg aattgtatca tcatctagat ttggtttgcg atcgatgtct
    81661 tcagtcttgc ttgtacccat tgtatcagtc tcgccaggta cgtccatcgg atcagttcct
    81721 ggtgtcatat tcaatggatc cgttgtattt tgaaagcctg tcgtgccacc tacgtccatt
    81781 ggatctgttc cagtgccagt ttctaatcca gtccccattg tgcttgctgg gctaacagta
    81841 tcttgtgtat ccattgtatc ttctgttgtt tctggctgac gatagccttt tactgtgatg
    81901 acaaattttc catcagaaat atctcctcta tattgatata aaggatcatt ttctggtgta
    81961 gccgtctcag aatcataggt atcaaaagaa aaagtatctt ttattttttc ccataaagaa
    82021 cggtcttctt caccatccat ccgattttca tctgtttcaa catctatacc agacaatccg
    82081 tcaaatccta gtctagatgc tgcttcatca gtagtataaa taacgatatc atctcgttca
    82141 tacccttcgt tacgcaaccg ttcaattact gataatgctt cttccctcgt actatagctt
    82201 ccgatgaccg ttaaattttt ctcttccatg taaattcctc ctagcaaata gatttatgat
    82261 tggtatattg ttacttataa atttattcta taggagacga ttgttttcta caaatattta
    82321 gattggaatt tgattaaaac aatttaattg aaatttgaaa acatttatgt taatgaaagt
    82381 catattctaa taaaatgtca tttgtttcta tatgaatttt tatgaaaaat ttcatataat
    82441 ttccactcgt tgtgatggtt taatttatcc aatccatcaa taagcaaaca acttatataa
    82501 aaaaggatta attctcaata taattgctcc taccatagaa taaatcttca tgtaaagtaa
    82561 tgaagtacca actatttcta atacttcttt aagaattatc ccaacaatat cgttagacaa
    82621 accattattg aataaatgga gagccaatcg attctgaaaa aatgttttct aatttttcgg
    82681 aaataaaaga aagagcctcc tgccattaga acgaaccaaa tccatttcat tgctcatgaa
    82741 gtaaccacat agctaaaaag aggataaatc aattaaaaga tcaaaagaat gactgcacta
    82801 ataaaataac taaaaatgta tcttcttact tcatatcttt tagctagtac ttagttaaaa
    82861 atttgcttcc taagatcaag acaaactgtc tatcttcatt tgatgaaatg cggtaaaata
    82921 tagaaaatga acactattgg agggaatttg taatggacag caaaactaga aaacaactgc
    82981 aagaaaagtt aaagcaacgt acacacttgc tcccttccgt ctttcaacag tttttagaag
    83041 aaaatcgttc ttccctttca ttaggcagtc gctatgaata cgcaaaagat tttcatctgt
    83101 ttactgatta tttgagaaaa gtattttctg aagaagtgga tgatcagctg attatccatt
    83161 tagaaaaaca agattatcag aatttcttag ctaaaatcta tcgacatact cgttcgttcc
    83221 aaactacagc gaaccaagaa acggagcaat tgtttgaaaa tagctattac gggatttcaa
    83281 ggaagcaata ttcattaaat cattttttac gatttttgta tcaaaaagga ctgattgaag
    83341 aagagatttc tcttttgggt acaagtttac cggaaacaac caaagcagct ccgcgatata
    83401 tcgatgcaga acttttcaaa gattttgagc atttgtttat cccgattgaa ggctttcaaa
    83461 caagcagaga agctagcttt gaagaaaaga atagaccgag gaaccttgca attactgcca
    83521 ttcttctgta ttgcggatta aagatccatg aagtagtaga acttaaacgt aaagatgtat
    83581 ctttgaccaa gcagattctt aatctgaata gaaaagaaaa cagactgaat aaagtaccaa
    83641 taccaaaaga agcgatgcca tttctggaga actattttaa gttaacagca caaaatacgg
    83701 acgaaaattc ttttgttttt cgttcctcac atgatcagca aatttctcct cggactattc
    83761 gccaaatttt gagcaaaatt acagtatata aaaacgtatc gccagaacta tgcagaaaaa
    83821 catatcgtta ttatgcagaa ctatatttag atgatgctga cgcggtaaat tatttatgtg
    83881 gtaatcgata cctctcatcc cattcttttc aagatatctg ggaaaagatg gttgattttt
    83941 cttatcacga attagcggca ttcgttcggg tttcttccat ataaaacaaa tgtatgatct
    84001 ttttttcaca ataaactatg ttattatata aaggattatg ttacgattat cttgaatttg
    84061 tatattactt aacaaaagat aatggagtgt gaatgatgga gaccataacg tattcccctt
    84121 tgaacttatt tacgaatttt caagaagctg cagctaactt ttccgagacc ccgattatct
    84181 ttgatgagcc actttcagct ttcccagaat taggacttga aacaacttac aaaaaatgct
    84241 gcgaaagtgt gttgaaacgg gcttatcaat tggctcatct tggagtgaaa tccggagaca
    84301 aagttattat ttataagagt tccgcttttg acacatacct tctagctgtt tctgtgtctt
    84361 accttggtgc agtgcctgtc atgacttctt atcatctacc aacaacaaca atggaagtat
    84421 ttatcgatcg tttagaagac cctttcattt tatttgatga tgagacaaaa aagcgagtga
    84481 gagaaatttc aaatggaaca aaatcaaaac agattc
//
LOCUS       DACDUU010000003        82201 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.003, whole genome shotgun sequence.
ACCESSION   DACDUU010000003 DACDUU010000000
VERSION     DACDUU010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 82201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.003"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(190..552)
                     /locus_tag="IWL30_000163"
     CDS             complement(190..552)
                     /locus_tag="IWL30_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAP9704963.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(589..804)
                     /locus_tag="IWL30_000164"
     CDS             complement(589..804)
                     /locus_tag="IWL30_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9704964.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            931..1266
                     /locus_tag="IWL30_000165"
     CDS             931..1266
                     /locus_tag="IWL30_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9704965.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(1342..3339)
                     /locus_tag="IWL30_000166"
     CDS             complement(1342..3339)
                     /locus_tag="IWL30_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9704966.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(3437..3712)
                     /locus_tag="IWL30_000167"
     CDS             complement(3437..3712)
                     /locus_tag="IWL30_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9704967.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            3944..4678
                     /locus_tag="IWL30_000168"
     CDS             3944..4678
                     /locus_tag="IWL30_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9704968.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            4717..5859
                     /locus_tag="IWL30_000169"
     CDS             4717..5859
                     /locus_tag="IWL30_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9704969.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(5954..7345)
                     /gene="sufB"
                     /locus_tag="IWL30_000170"
     CDS             complement(5954..7345)
                     /gene="sufB"
                     /locus_tag="IWL30_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAP9704970.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKAVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(7350..7820)
                     /locus_tag="IWL30_000171"
     CDS             complement(7350..7820)
                     /locus_tag="IWL30_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAP9704971.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(7807..9042)
                     /locus_tag="IWL30_000172"
     CDS             complement(7807..9042)
                     /locus_tag="IWL30_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP9704972.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(9039..10325)
                     /gene="sufD"
                     /locus_tag="IWL30_000173"
     CDS             complement(9039..10325)
                     /gene="sufD"
                     /locus_tag="IWL30_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAP9704973.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(10338..11111)
                     /gene="sufC"
                     /locus_tag="IWL30_000174"
     CDS             complement(10338..11111)
                     /gene="sufC"
                     /locus_tag="IWL30_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAP9704974.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(11308..12156)
                     /locus_tag="IWL30_000175"
     CDS             complement(11308..12156)
                     /locus_tag="IWL30_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9704975.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(12180..12866)
                     /locus_tag="IWL30_000176"
     CDS             complement(12180..12866)
                     /locus_tag="IWL30_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9704976.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(12859..13893)
                     /locus_tag="IWL30_000177"
     CDS             complement(12859..13893)
                     /locus_tag="IWL30_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9704977.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(14288..14626)
                     /locus_tag="IWL30_000178"
     CDS             complement(14288..14626)
                     /locus_tag="IWL30_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAP9704978.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(14628..14984)
                     /locus_tag="IWL30_000179"
     CDS             complement(14628..14984)
                     /locus_tag="IWL30_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAP9704979.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(14990..16177)
                     /locus_tag="IWL30_000180"
     CDS             complement(14990..16177)
                     /locus_tag="IWL30_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP9704980.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(16368..17213)
                     /locus_tag="IWL30_000181"
     CDS             complement(16368..17213)
                     /locus_tag="IWL30_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9704981.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            17447..18412
                     /locus_tag="IWL30_000182"
     CDS             17447..18412
                     /locus_tag="IWL30_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAP9704982.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            18789..19832
                     /locus_tag="IWL30_000183"
     CDS             18789..19832
                     /locus_tag="IWL30_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAP9704983.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(19894..20358)
                     /locus_tag="IWL30_000184"
     CDS             complement(19894..20358)
                     /locus_tag="IWL30_000184"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAP9704984.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            20604..21551
                     /locus_tag="IWL30_000185"
     CDS             20604..21551
                     /locus_tag="IWL30_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9704985.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(21762..23834)
                     /locus_tag="IWL30_000186"
     CDS             complement(21762..23834)
                     /locus_tag="IWL30_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9704986.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(23767..24144)
                     /locus_tag="IWL30_000187"
     CDS             complement(23767..24144)
                     /locus_tag="IWL30_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAP9704987.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(24273..24833)
                     /locus_tag="IWL30_000188"
     CDS             complement(24273..24833)
                     /locus_tag="IWL30_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9704988.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            24983..26275
                     /locus_tag="IWL30_000189"
     CDS             24983..26275
                     /locus_tag="IWL30_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9704989.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(26335..27453)
                     /locus_tag="IWL30_000190"
     CDS             complement(26335..27453)
                     /locus_tag="IWL30_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAP9704990.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(27467..28888)
                     /gene="arcD"
                     /locus_tag="IWL30_000191"
     CDS             complement(27467..28888)
                     /gene="arcD"
                     /locus_tag="IWL30_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAP9704991.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(29134..30255)
                     /gene="mnmA"
                     /locus_tag="IWL30_000192"
     CDS             complement(29134..30255)
                     /gene="mnmA"
                     /locus_tag="IWL30_000192"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAP9704992.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            30659..31258
                     /locus_tag="IWL30_000193"
     CDS             30659..31258
                     /locus_tag="IWL30_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAP9704993.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(31396..32682)
                     /gene="efmA"
                     /locus_tag="IWL30_000194"
     CDS             complement(31396..32682)
                     /gene="efmA"
                     /locus_tag="IWL30_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAP9704994.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(32953..33297)
                     /locus_tag="IWL30_000195"
     CDS             complement(32953..33297)
                     /locus_tag="IWL30_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAP9704995.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(33434..34570)
                     /locus_tag="IWL30_000196"
     CDS             complement(33434..34570)
                     /locus_tag="IWL30_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP9704996.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(34759..35733)
                     /locus_tag="IWL30_000197"
     CDS             complement(34759..35733)
                     /locus_tag="IWL30_000197"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP9704997.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(35813..36688)
                     /locus_tag="IWL30_000198"
     CDS             complement(35813..36688)
                     /locus_tag="IWL30_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAP9704998.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            36818..38725
                     /locus_tag="IWL30_000199"
     CDS             36818..38725
                     /locus_tag="IWL30_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAP9704999.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(39266..42544)
                     /locus_tag="IWL30_000200"
     CDS             complement(39266..42544)
                     /locus_tag="IWL30_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAP9705000.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(42815..43360)
                     /locus_tag="IWL30_000201"
     CDS             complement(42815..43360)
                     /locus_tag="IWL30_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9705001.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(43372..44064)
                     /locus_tag="IWL30_000202"
     CDS             complement(43372..44064)
                     /locus_tag="IWL30_000202"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAP9705002.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(44083..44367)
                     /locus_tag="IWL30_000203"
     CDS             complement(44083..44367)
                     /locus_tag="IWL30_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705003.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(44380..44943)
                     /locus_tag="IWL30_000204"
     CDS             complement(44380..44943)
                     /locus_tag="IWL30_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP9705004.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            45171..45833
                     /locus_tag="IWL30_000205"
     CDS             45171..45833
                     /locus_tag="IWL30_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAP9705005.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            45957..47153
                     /locus_tag="IWL30_000206"
     CDS             45957..47153
                     /locus_tag="IWL30_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAP9705006.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(47254..48630)
                     /locus_tag="IWL30_000207"
     CDS             complement(47254..48630)
                     /locus_tag="IWL30_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9705007.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(48667..49533)
                     /locus_tag="IWL30_000208"
     CDS             complement(48667..49533)
                     /locus_tag="IWL30_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAP9705008.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(49622..50647)
                     /locus_tag="IWL30_000209"
     CDS             complement(49622..50647)
                     /locus_tag="IWL30_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9705009.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(50665..51339)
                     /locus_tag="IWL30_000210"
     CDS             complement(50665..51339)
                     /locus_tag="IWL30_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9705010.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(51523..52416)
                     /locus_tag="IWL30_000211"
     CDS             complement(51523..52416)
                     /locus_tag="IWL30_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9705011.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(52490..54187)
                     /locus_tag="IWL30_000212"
     CDS             complement(52490..54187)
                     /locus_tag="IWL30_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9705012.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(54180..55493)
                     /locus_tag="IWL30_000213"
     CDS             complement(54180..55493)
                     /locus_tag="IWL30_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP9705013.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(55493..59011)
                     /locus_tag="IWL30_000214"
     CDS             complement(55493..59011)
                     /locus_tag="IWL30_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP9705014.1"
                     /translation="MDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEK
                     KEISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMV
                     NFYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETL
                     YKDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDV
                     LFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGS
                     SNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAI
                     GRNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVS
                     PFGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYK
                     FSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSS
                     SIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFET
                     KMPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEY
                     TSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNI
                     NKLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDG
                     KYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRL
                     YDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTL
                     DGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQP
                     LHLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIID
                     GVLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPND
                     SSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTL
                     KVEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYIL
                     LAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(59304..59762)
                     /locus_tag="IWL30_000215"
     CDS             complement(59304..59762)
                     /locus_tag="IWL30_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9705015.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(60059..60577)
                     /locus_tag="IWL30_000216"
     CDS             complement(60059..60577)
                     /locus_tag="IWL30_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP9705016.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(60579..61196)
                     /locus_tag="IWL30_000217"
     CDS             complement(60579..61196)
                     /locus_tag="IWL30_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP9705017.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(61193..62047)
                     /locus_tag="IWL30_000218"
     CDS             complement(61193..62047)
                     /locus_tag="IWL30_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9705018.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(62044..62955)
                     /locus_tag="IWL30_000219"
     CDS             complement(62044..62955)
                     /locus_tag="IWL30_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9705019.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(62996..64330)
                     /locus_tag="IWL30_000220"
     CDS             complement(62996..64330)
                     /locus_tag="IWL30_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9705020.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            64538..65368
                     /locus_tag="IWL30_000221"
     CDS             64538..65368
                     /locus_tag="IWL30_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAP9705021.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(65612..65861)
                     /locus_tag="IWL30_000222"
                     /pseudo
     CDS             complement(65612..65861)
                     /locus_tag="IWL30_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(66052..66246)
                     /locus_tag="IWL30_000223"
     CDS             complement(66052..66246)
                     /locus_tag="IWL30_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705022.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(66751..66936)
                     /locus_tag="IWL30_000224"
     CDS             complement(66751..66936)
                     /locus_tag="IWL30_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705023.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(67786..68127)
                     /locus_tag="IWL30_000225"
     CDS             complement(67786..68127)
                     /locus_tag="IWL30_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP9705024.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(68127..68357)
                     /locus_tag="IWL30_000226"
     CDS             complement(68127..68357)
                     /locus_tag="IWL30_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9705025.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            68644..68982
                     /locus_tag="IWL30_000227"
     CDS             68644..68982
                     /locus_tag="IWL30_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705026.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(69187..69426)
                     /locus_tag="IWL30_000228"
     CDS             complement(69187..69426)
                     /locus_tag="IWL30_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAP9705027.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(69402..69749)
                     /locus_tag="IWL30_000229"
     CDS             complement(69402..69749)
                     /locus_tag="IWL30_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAP9705028.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(70481..71062)
                     /locus_tag="IWL30_000230"
     CDS             complement(70481..71062)
                     /locus_tag="IWL30_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9705029.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(71245..71871)
                     /locus_tag="IWL30_000231"
     CDS             complement(71245..71871)
                     /locus_tag="IWL30_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9705030.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(71893..73224)
                     /locus_tag="IWL30_000232"
     CDS             complement(71893..73224)
                     /locus_tag="IWL30_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAP9705031.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(73379..73780)
                     /locus_tag="IWL30_000233"
     CDS             complement(73379..73780)
                     /locus_tag="IWL30_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP9705032.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(73866..74072)
                     /locus_tag="IWL30_000234"
     CDS             complement(73866..74072)
                     /locus_tag="IWL30_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705033.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(74167..75669)
                     /locus_tag="IWL30_000235"
     CDS             complement(74167..75669)
                     /locus_tag="IWL30_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAP9705034.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(75687..76142)
                     /gene="argR"
                     /locus_tag="IWL30_000236"
     CDS             complement(75687..76142)
                     /gene="argR"
                     /locus_tag="IWL30_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP9705035.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(76240..76857)
                     /locus_tag="IWL30_000237"
     CDS             complement(76240..76857)
                     /locus_tag="IWL30_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705036.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            77078..78451
                     /locus_tag="IWL30_000238"
     CDS             77078..78451
                     /locus_tag="IWL30_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9705037.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            78526..79638
                     /locus_tag="IWL30_000239"
     CDS             78526..79638
                     /locus_tag="IWL30_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAP9705038.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(79710..81347)
                     /locus_tag="IWL30_000240"
     CDS             complement(79710..81347)
                     /locus_tag="IWL30_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAP9705039.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(81515..82123)
                     /locus_tag="IWL30_000241"
     CDS             complement(81515..82123)
                     /locus_tag="IWL30_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9705040.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT        23492 a        17348 c        14487 g        26874 t
ORIGIN      
        1 ttcttgtaaa atctgtgtta ccaatggttt tgatgacttc tctaatccag atgtacctgt
       61 catttttgaa aaaataatac ctataatagc caagacaaca atacctataa tgacaaactt
      121 cttgttcata tttactttat tcaacttttt atctcctatc tccaaaataa tttgttctta
      181 tccctcttat cagtacattg acatccctaa aagtacacca aagattgctc cagctacccc
      241 tgctatcatc aaaatcgtac ccattttctc atctttttct cgatataagt agcccataat
      301 aacagcaaca gcaccaaata caacaggaat aaatgctaac gatactacca tagaaatcca
      361 accaataatc aaatacagct tccctgtctc ttgtccatgt acgatttgtt ccttcatttc
      421 attaattgtc tgctcagtac ttttctttac tgtgtctgtt ccggttgtct catctgtttc
      481 ttgtagtgct ccacaaaact ggcaatactt actttcatct gttatttctt tgccacattt
      541 tctacaatac atgtcttctc ctcctaaaat cattcaattg cttattagtt aactgtttac
      601 tttattaaat acatcagaaa acagctcatt cattggtaca cttaatatct cagaaagcat
      661 cttcatttga tagggtttag gaatcgttct ccctgtttgc cacgaactta cacattgctg
      721 tgtaacattc attttttttg ctaactccgt ttgagaaagt cctttttctt ttctgtatcg
      781 gctgagatta cttacttttt tcatgattct cccctcctgt tttattatat acaacaatat
      841 gatgtatttc aactcataaa atataaaaaa cttcaaatac cattacaaat acaacaactt
      901 gttgtactat actgagatgg gggtgtatta atgaaacaga aacaatatgc aaaggtcatt
      961 agagaaaagc gaaaagctct cgggtggaca caaaaagaat tagctgaaaa aatcttctct
     1021 acacaacagg cagtagcaag atgggaaaat tctgtaacag agcctaacct cgatagctta
     1081 acagctcttt ctagagccct aggaacccct gtaagccatt ttctagacaa tgttgtcgta
     1141 gattatgagg aagagttttt ggctctctat cgttctctta gcactgaaga tgcagttcga
     1201 acaattgact atatgaagct attaaaaagg caagaaaatg aacgcaatca gctattgaaa
     1261 gattgaaaaa taaataaccc accgaacttt aacggtgggt gtattgagat gaaataagat
     1321 taagagctat ggataacctg tctagtttaa tacaattggg tcttttctct tccttactct
     1381 ccgtccatca tctcttgtta cacttgtaaa ttgatacaga ggaactatcc cagctcctga
     1441 taatatcaat tcttttattg tttcatctat ccctgcccct ttcgctaaat atattaaaaa
     1501 actactactt gctttcacat tgtatagttt cttaatatcc ttagaagctc gttcaagagt
     1561 gaatccttgt ttaacaaagt agcctctgtt ttttattaaa gcatttttca cgctgttatc
     1621 cccaacatta cgtaggcgtt cacaaacagt gttaataagt tctttctctg tcattagata
     1681 tgtcccttgc cacgataagc cctgtaaaac ttcatgtgct tcatctaaaa tcaattgttt
     1741 ttccttgctg agttctgatc gggaattttg aggaaatact cttttagcct ctttttcgtt
     1801 aaaacatatc ttaacccatt cataagaaaa atattgatat gtaaaatgaa ttttttccag
     1861 aagtggaagg atatcttcat tcaatttttg aatcacagaa gttgcattaa aaacgtctaa
     1921 tcgatagaca ttgcttctct tatgattaaa gtttcctaac gctttctctt cctttaacct
     1981 atttaggtat tcgagcatct tcaaaggaac atcttcatca tctaattttt ttatcaatcc
     2041 tataaaagtc ataagtgata gtaacttatt acatttatct atgtcataag ttttaccata
     2101 gtaagaactc atttttttag ctaattgttc ccccgacaaa atacaagtca cactttcctt
     2161 actatcatca aaaaaattaa catcagaaag caagacatca tagacacgtc gcatttcctc
     2221 tagataattc cttattgtcc tgaaaagttt gtctttcatt gattcaagag tgtcaaaaag
     2281 tacttttgat tcctctttca attttcgaaa atgtgtagga tacaagtcta ggttctctaa
     2341 tagccaatta aatgtttcta cccgactgtc tttctttaaa attagcatca caactccaat
     2401 aaaattttca ctgatatcac aattactgga atgacaataa aaaatggaat gcccagtttt
     2461 actggtaaaa atactagcag agggattatg atcgttgtga aacagacatc ttaagttttt
     2521 acttgactta agtaatttat tcatatcaat ggttaacaat ctttcataaa tctcattaaa
     2581 agacatgtcg gaggaatcaa acaggcagtc tttaaaccac tgtttcattt cttctttgtc
     2641 gtcatttttt atcaacttaa caaaatcggt ttcattgact agttcatact ctctccctga
     2701 atcactttga caatttttag aacatgtcat ggtaaaatca atcggaagct cagtaatatc
     2761 taacatgttg tcttcattaa ttattatcac tgcatctgac ttatatcctc caaaaaacat
     2821 tctattcgaa gcatttacat tgtcatcaca cccggggatg ttctttttca agtattcgta
     2881 aacagcaacc attttaccat agtcagtaat tgcttctttt aatagaaaga caatcttgta
     2941 tttttctaga ttttcagagt accgaatact tttttgtatc gcaaacgcag aattatgaac
     3001 taaattgata gataaaacct tttctatgct taagtatcta ctatcatctt tactcaaatc
     3061 tactaccttc cccttctcat ctttaacagt gttatcgaca tccagaataa ttactctggt
     3121 ttccacaaaa ctctccttgt cattgccttt attctttgtc ttgcttggaa taaatgattg
     3181 tgatgaattt agtatctcta caaactcatc tcttgtaact tctctatagt tatctaaaca
     3241 cctatctctt acatcagttt tttctttttt tgtaactggc ttctctaata atttttcttg
     3301 atctacataa attggaatat taatcattga tgtttccatt tgtgtttcct ccttataaaa
     3361 ttctgttaaa gactaaaaca tacagataat aaaaacaaac tctttattac tatatgtttt
     3421 agtcttattt gtttactcaa cctttatacc gttccatgaa actatcaata tcagattttt
     3481 taaatctaat gaccgtatca atttttataa caggtaaccc gtattgagaa acatattttc
     3541 taatcgttcc tactgataca cctagatatt cacttgcctg ctgatagtcc atatactcta
     3601 gagtgactcc agaaataatg tcttctagca taggctttag cactgtaatt atctctttca
     3661 cttgttgttc aacttgtata gaaacaatgc tatcaatgat ttgtagagac atatttcccc
     3721 ctcctttcgt gattgcttga acaaattttc taagaattat cttcgtattt tttgctcaaa
     3781 ataaaagtat tttgttatta accgattaat caaacaaaaa atgattacca gttcatcgac
     3841 atacacataa tacctcgata aaaaaggggt gtcaacagct ttgtaaaatt tatttacaac
     3901 accccttttt tgaggtatac taattttagg aggtgtggtc aatatgataa aacgtccacc
     3961 aataaattat ttagaacgaa aaaagatact cggaacaaaa ataaaagcta taagaaagag
     4021 taaaaaatta acacaaccag catttggtct tatgattaac aacggtcaat taattgacaa
     4081 aaaaactatc tacgaatggg agaagggaac ctatttacca ataccagagc gattatcccg
     4141 aattgctgat ttgggaaata tgagtataga ggagttagtt tgtggcaatg tagaagaata
     4201 tattttggga attattcttt atagagatag cattgtacta gatggtataa catttccaga
     4261 taaaaacctg ttccagcatc ttcgtcaaca gttcccacca gtacactcta atttagatac
     4321 atggctggat agatattcca aattagaacc agagatgcaa gaatttattg ctaacaagac
     4381 ttgtaataaa gttaaaaatg aaaaaattag tcttttcaat atcttaaaaa tagaagaatt
     4441 attcatcaat gctattgtag aagaatttga caataacatt cttttcctca ctagcagtat
     4501 agaagaacta ctagaaagaa tggtagatga atggctacct attcaattga aagacatgtc
     4561 ttatcccgaa gaagctgtga gagaaattac tgataatata aacaagctag aacaaacgat
     4621 ttcatcaatt gggaaaaaat acactaagaa aaaaatgaag ggaggtgata ccatctagtg
     4681 gtaagtactc cttgtaaact aagaggagat gatttaatgg caaaaatatc aaagtacaaa
     4741 aagaaaaacg gtcaagtggc gtggatgtat aaaggacaca ttgccaccga tccacgaact
     4801 ggtgaaaaaa taaatactac gagaagaggt tttagtagca aacaagaagc tcaaaaagat
     4861 tatgacgagt acagacatca aatattatac ggtgttaaaa agaaagcacc tgatatgaca
     4921 tttgaagacc tgtataatga atggataact catcagcgta ccagtgttaa agcttcaaca
     4981 atcgcaatct cagtaagata cgcaaataat caaatacttc cagcatttgg taagcttaaa
     5041 ctatcgaata tctctgtccc atattgtcaa aaagtggtag atgaatggca cagtaaatat
     5101 gaaagttacg attatatgag aaaacagact gcacaaattt tacgctacgg ggttgcaatg
     5161 caatatattg acaataatcc tatggaaaaa acacttcttc cacgaaaaaa agagtatgag
     5221 aaaaatcgta aattttactc aaaagaggag cttaataatt tactggatgc ttttaaagat
     5281 tttggtaaca tgaaacaata cgccttcttc agattattgg catacacagg tatgagaaag
     5341 agtgaagtac tcgctttaca gtggaaagac atagacacgt ttaataaaga actacatgtc
     5401 aataaaactc ttgctgtaga tgaatttggt aaagttataa ttcaatctcc gaaaacaaga
     5461 gcttctagac gagttatctc tttagatact gagactcttt ctatcctcaa caactggaaa
     5521 ttacagcaaa aagaagagta tttaaaactt ggatacaata ctagctctaa agagcaacat
     5581 gtttttacaa cagttaaaaa cactctatac attccaaaca ctgttaatga ttggttaaga
     5641 tatattttga aaaaatataa cttgcctcgt attacacctc atgggtttag acatacacat
     5701 gctagtttat tattagaagc aggagaatct gtcaaggtag ttcaacaacg attagggcat
     5761 gaaaatagta aagtgacatt agacatctat gcacatatta caaataatgc acctaagaaa
     5821 acaggtcaag actttgctga tatgatggct catcagtaaa agagctggtc aaaatctggt
     5881 catttctagt atacaggcaa aacaaaaaag ctagaaccct tatatatcaa gggttctagc
     5941 tcctataaca ggtttaccca acactgcctt ccatctcata actgatcaat cgattgagtt
     6001 cgactgcata ttccattgga agttctttgg tgaatggttc aacaaagccc atgacgatca
     6061 tttcagttgc ttcagattcg gtcaggcctc ggctcatcag atagtagagt tgttcttctg
     6121 agatttttga gactttggct tcgtgttcta aagagacttg gctgttgtgg atctcattga
     6181 atgggatcgt atcggatttc gaccattcgt ccatgatgat cgtgtcacat tcgatatgag
     6241 agatcgaacc cgcactgttt ttggcaaagg ttacttgtcc tcgatagttt acttctccgc
     6301 cgtcttttga aattgattta gagacgatag agctagatgt atttggtgca ttgtggatca
     6361 tttttgcacc cgtatcttgg atttggtttg ctcctgcaaa ggcgatggag agcatggtgc
     6421 ctcgtgcacc ttttccatct aagtaaacac ttgggtattt catcgttgtt tttgcaccta
     6481 ggtttccgtc gatccattca acggttgcgc cttcgtatgc ttttgctcgt ttggttacta
     6541 agttgtacac attgtctgac cagttttgga tcgttgtgta acggcaatac gcatcttttc
     6601 ttgtaaagat ttctacgatg gcggcatgca ggctgttact tgaataagta ggtgccgtac
     6661 aaccttcgac atagtggatg cttgctcctt cgtcaacaat gatcagcgta cgttcgaatt
     6721 gccccgtgtt ttccgcattg atccggaagt acgtttgtaa tggaacatcg actttgacgg
     6781 cttttggcac gtaaataaag gtcccacctg accatacggc tgaattcagc gctgctaatt
     6841 tgttgtcggt tggcggtaca agttttgaaa agtactcttt gaataattca gggtattctt
     6901 tcaaagcaga gtcagtgtca gtaaagacga ttcctaactt ttcgaattca tctttcatat
     6961 tatggtacac gacttctgat tcatattgtg cggaagcacc ggctagataa gctcgttcag
     7021 cttctggtat accaatcttt tcaaatgtat ctttgatttt ttctggtacg tcttcccagt
     7081 cacgagcggg tttgtcactt ggtttttggt aatatttgat ggcatcgaaa tcgatgtctg
     7141 aaaggtcggg gccccatgtt tgcattggca ttttgttaaa tgtttctaga gatttcagac
     7201 ggaattcaag catccattct ggttcgcctt tgattcttga catctcacgg atcacttctt
     7261 ctgtcaagcc ttctcctgta ctgaaaaccg gttcgacatc atcgtgaaac ccgaatttat
     7321 attcttctaa ttctggtacg cccatgttct cactcctctt ccttgtggta atgatggaaa
     7381 gctgcggtac catcgttttc cattgctttt cctagtgctt tccaggcaag tgtcgcacat
     7441 ttgatccgag cgggaaattt agctacacca ccaagcatag cagcatcgcc taattggtcg
     7501 ggatctttga cttcttttcc ttggacaagc tgtagaaagt ctacagttaa atgttctgct
     7561 tcttctattg attttccgat gacggcgtct gtcatcatcg aggcacttgc cgtactgatg
     7621 gagcagccac tgccgtcaaa ggcgatattt ttgatgattc cgtcttttac atcgacttgc
     7681 aattcgatca catctccaca tgtggggttg ttcaattcga tcttgcttgt cgagtcagtc
     7741 aagcttccac gatgatgcgg atgggaagaa tgatccaaga tcacttgacg gtataaattg
     7801 tctaatttag atagtcccat gttggaaaaa ctcctttgtt gccatgattg cttcaactaa
     7861 acggtcggcg tcttgcgttg tgttgtagaa ataaaagctc gcgcgagcag tcgctgatac
     7921 ttcaaggtac ttcaatagcg gttgcgcaca atgatgacct gctctgacgg ctaccccttc
     7981 catgtctaaa gctgtcgcga catcatgggg atgtaaccca tccaaattga atgcaacgac
     8041 tcctgtccga aaattcgggt cttgtggacc ataaacagtc aatccatcaa ttgcttgtaa
     8101 ttttggcaac acataagcgg ctagttcttc ttcatatcga tggatattgt ccataccgag
     8161 tttttgcaaa taatcgattg catggcctaa tgcgatagcg ccggcgatat ttggtgtccc
     8221 tgcttcgaat ttccaaggta gttctttcca tgtactgtcg tataattcga caaaatcgat
     8281 catttcgcca ccaaattcaa caggttccat ttcttctaac cattttcgtt taccatataa
     8341 aacaccaata cctgttggtc cgcacatttt gtgtccgctg aaagcaaaga aatcacagtc
     8401 tagtgcttgg acatcaatgg ccatgtgcgg ggcagattga gcaccatcta ctacgatgac
     8461 cccgccgttt tcatggacaa gttcagctaa ttcttgaaca ggattgaaga cacctaagac
     8521 attggataca tgagcaatgg agacgatttt ggttttgtcc gtaatctttt tcgctgcatc
     8581 ctccatatcc aggaagcctt cttctgttag ttcgatatat ttcaagacag cacctgttcg
     8641 ttttgccaac tgctgccacg ggataacatt agaatgatgt tccatgtaag agagcactat
     8701 ctcgtctcct gctttgatat agcggtcgcc gaaggttctg gctacccaat tcaagcttgt
     8761 cgtcgtacca cgagtaaaca gcacttctgc tgtttctttt gcatggatga acgcccgtac
     8821 tttttctctt gcttgttcat aatcatgggt cgcgcgttct gctaacgtat ggacaccgcg
     8881 atgaacattg gcgttatcgt gttcgtaata gtacgctaat gcctggagta cttgttttgg
     8941 cttttgggtc gtcgctgcat tatccagata aactaaaggt tcatcattga cgtcttgaaa
     9001 taagatagga aaatcattgc gtagcttttt agcatcgatc atccattcaa cttcccttcg
     9061 attacttcga ctaattcttt ccgaacttgt tctacaggga ttgctgttaa gacagaaccg
     9121 aggaatcctc gaatcactaa gcgctctgct tcttctttat gcaatcctct actcatcaga
     9181 tagtacattt cttctggatc aactcgtcct acactggcag catgtccagc agttacttcg
     9241 ttttcatcga tcaaaagaat aggattcgca tcaccacgtg ctttatcaga aagcatcaag
     9301 acacgacttt cttgttgggc atctgcacct tttgcccctt tcaagatgtg gccgatacca
     9361 ttaaaggtca atgttccttt ttctcggatc actccgtgtt gcaaaatatg cccgatcgta
     9421 tgaggggctt tattggttac tcgtgtatcg attccttgga tttgtctgcc cgagctgatt
     9481 gccacgattt tgacttctgc gtgtgcacct tctcctgcta aatctgagtc aaagtcagcg
     9541 atcacatgac cgtcattcat gacaccaatt gcccagtcga cagaagcatc tcgtaaaatg
     9601 tgcccacggc gattcatata agaagtaata ttttctccta attgatctac ggctgaatat
     9661 ttgatttttg aaccagcttt agcgatgact tccacgatga tattacctga tgattttgct
     9721 acttgttctt tggttgtctg gaaacgttcc agataagaaa attcactatg ttcatctgcc
     9781 acgatcaaca catgcttgaa gaagtgctca tcggatgcgc catcttgaat gaataaggac
     9841 tcgagtggtt catcgatcac gacattttta ggcacataca ggaaaatacc gctgttcata
     9901 aaagctacat ggagtgcagt caattggtct tcgttcatgt ttactgcttt tttcatataa
     9961 tattcttgga ctagttctgg atattcgatc attgcagtga acaagtcggt aaaaatgact
    10021 cctttttctg ctaattccac aggcagttgt tcaaaaatcg tccatgaacc ttgttggaca
    10081 agcacaggat tgtctttcat tgcatcaaat gccggaatcg tgcctgtttc actgatggtt
    10141 tgtgtttctt tcacatcaaa taacgaccag cgatggaatt tcacccgatc gatatgcggc
    10201 aatggcagtt catctgcttt cgctagtgcg tcttgacgga aggttctcat ccaatcaggt
    10261 tcgcctttac ttaaagaaaa atcatttacc gcgtcaagat aatcgagcca gtttttttct
    10321 ttcattcttg ctccccctta cgcttcttct tctttatagt cgattcctaa ttctttactg
    10381 ataccagcgt agccttctgc ttccagacgt ttagctaggt cagcattccc tgtcatgact
    10441 actcggcctt ccatcatgat gtgaacgaca tcaggtgtga tatagttcaa taatcgttga
    10501 tagtgggtga tgatcaatgc accgaaattg tctccgcgca tttcatttac tcctttagca
    10561 acgactttca aagcatcgat atccagacct gaatcgattt cgtctaaaat tgcaaatgta
    10621 ggttctaaca tcaacaattg caggatctca ttccgtttct tttctccgcc agaaaaacct
    10681 tcgttcagat aacgttcagc catttcttct ggcatattta gtaaagccat tttttcatct
    10741 aactttttca aaaatgccat aacagaaatt ttgtcgtttt catcacgttt cgcattgatc
    10801 gctgcacgca tgaattccgc attcgtgata cctggtatct cacttggata ttgcatagct
    10861 aaaaataagc caagtcgcgc acgttcatct acttccaagt ctaaaacatt tttcccgtca
    10921 aacaaaattt cgccttctgt tacttcatag tttggattgc ccatgatagc ggcagataag
    10981 gtagattttc ctgtgccgtt aggtcccatg atggcatgga tttctccggt tttcattgtt
    11041 aggtttacgc ctttaaggat ttctttttcc tcgatcgaca catgtagatt ttttatttct
    11101 aagacagaca tgcattatac ccccaaattc tctttcgttc tcactcattt tagactaatt
    11161 gagaatgaag cgcaatgacc ttccttacta aaatcaaata tcttttattt attattaaac
    11221 aattttgatt tctaaattag aataggtata aaaaagatga gaaataagcc attacagact
    11281 tatttctcat cttttttgtt ccatacctta ttttgcatca gcctttactg gtttaacaga
    11341 gccgccccat ttgtctgtga tccattcttg gacttctttg cggtgaagca cttcaatcag
    11401 ttttttgatt ttgtcactgt cttcatcacc tttacgagtc gcaataatat tcacgtaagg
    11461 agagttgtcc ttttcaagca atacggcatc ttctaatgga ttcaaccctg cgccgtaagc
    11521 gaagtttgcg ttgattgcaa ctaggtcacc ttcatcattt tggtacgtag aagctaaaag
    11581 tgatgggtcg atcgtatggt tgaacttcaa gttcttaggg ttttcttcaa tatcatcgaa
    11641 tgttgctgtt tccagatcta ctccatcttt gactttgact aagccagcat cttgtagaat
    11701 tgtgatgatg cgtccccagt ctgattcaga atttgaagta atgattgttg caccatcttt
    11761 taattcggaa acatctttga cttttttaga gtacaagccc attggttcga tgtggacagc
    11821 accggcattg acaaagtcat atcctttttc tttgatttct ttgttcaaat aaggaatatg
    11881 ctggaagtaa ttggcatcaa tgtcgccttc ttccaaagat tggttcggta atacatagtc
    11941 atcgaatttg acgatttcaa gatcgattcc ttcatctttc aataatggtt tcacatgttc
    12001 caaaatctct gcgtgcggtg tcggtgaagc gccgactttc aatgtcgtgt cgtctgcttt
    12061 atttgctgaa tctttgctgt cgctgttgtt accgcatgct gctaatccta cagtcagcaa
    12121 aagcgctgct gcaaatccaa aaattttctt tttcatatcc tacatccccc tatatcctat
    12181 tatcttttgt ctgttttctt agttaagaaa tcaccgattc cttgaacgat aaatacgatt
    12241 agtaaaataa ttaaagtcgc aacgaatgtc actgttagat taccacgctg gtagccttcc
    12301 tgccaagcaa gacctccaag tccacctgcg ccgatcgcgc cggccatcgc agtaaatccg
    12361 atcatcgaga tcgttgtaac tgttaatcca gaaatcaatg cgggaatact ttcaggtatc
    12421 aagaccttcc agatgatttc ccagtaactt gctcccattg catctgctgc ttccagtaca
    12481 ccagggctga cttcacgaaa agcaatctct actagacggg cataaaatgg tgctgcggat
    12541 aaaacaagag ctggaatggc tgcttgcgca cctagcatag tgcctacgat tgctttagta
    12601 aatggaatga tcaaaaccat caggatcata aatggcgttg accggaaaat attactgatg
    12661 atcgatacga ttccataaat gattcgagca tacggtttgt ttttgcgtcc aatagagtaa
    12721 aggatcaatc ccaaaactaa tccgatgatc gtcactaaga ccattgagat taatgtcata
    12781 tatagtgtat caaggatagc ctgattcatc gtttccgggt tgatttgttt aaaatctaga
    12841 tatgtttctg aaaagctttt atccacgatg gatcacctca attcctactc cttgttcttt
    12901 gatgaacact tctgcttgat ctaattcttt ggtttcacca gtcatcaaga cagtcaatga
    12961 accaaaggaa ccttctttca cttgtttgat ctttccatac acaacgttga catcgatgtt
    13021 atagcgtcga atcgcttggg aaatgactgg ctcgttggca tttctttcgc taaaggtcaa
    13081 agttgccaaa cggccgcttg gattttcttt gatgagttca gctaatagtt ctgtcgaatc
    13141 agcttctggt tcgatgtctt gttgtacaaa gcgtttggtt acttcttttt tcggttgtct
    13201 gaagacagtc aatacgtctc cttcttctac gactttcccc aactccatca cagcgacttt
    13261 gttgcagatt ttgcggatca cattcatttc atgtgtgatc aagacgatgg tcaaattcaa
    13321 tttcttattg atcgctaaca ataagtctaa tacttcttct gttgtttgcg ggtccaatgc
    13381 gctagtagcc tcatcacaaa gcaggatctc aggatcattt gccaatgctc gtgcgatacc
    13441 gacgcgttgt ttctgtccgc cggaaagttg tgaaggatat gcttgtccgc gtccttctaa
    13501 cccgactaaa cgcaacagtt cttctgcccg ttctttacgt ttgctctttg gtacgccagc
    13561 taattccagc ggcagctgga tattttcaag tacagttctt gaccataaca ggttgaaatg
    13621 ctggaagatc ataccgattt tctttcggaa tgtacgtaat tctttgtttt ttaattttgt
    13681 aatatcttga tcgtttacga tcacttcgcc gctcgtggga agctcaagtc cattcaggag
    13741 acggactaat gtactttttc ctgcgccaga atagccgacg attccataaa tatccccttt
    13801 ttcaacgttt agtgaaacgt cattgacggc ttggatgttt ccgtttttcc cattgaatgt
    13861 ctttttgaca ttcgttagtt ggattaatga catgccattc acatctttct cttatgattt
    13921 ttcaaacaaa aaagccttcg ccctcttgcc ttaagcaaaa ggacgaaagc ttgaacttcc
    13981 gtgttaccac cttgatttac cgttacttcg caataacagc ctcttccagt acttggactt
    14041 agcttctttc cgtatactgt cgcgctgtaa cgggcactcc cgtagtaagt ttgcttacgc
    14101 tcgcttactc tgctctaaga ccatcttcca tctcattttc gttacccatt ttcagcaaac
    14161 agggctctct tcaaacgtcc gtgacatgta ctcttctttt caaagctttc tcgtttcata
    14221 taggattgct tatgattata gcaactggaa gaatcagtgt caactttttt caagtgattt
    14281 tctgatttta taattcgtca aattcttttg gatcaacatc tttgaaactt aagatccacg
    14341 cattcatttc atctgaatca ttcaagatat tgatatcttc accgatttta tcattaacag
    14401 aagaaactgt acccgttagc gggctggcaa attcattgac cgctttttcc gcttcaactt
    14461 cgatcagagg ttcatctttt tttagcgatt gtcctgcttt tggcagattg gcaaacgtga
    14521 tgtctccaag ttcttcttgc gcttcatttg tcaaaccaac acagtattct tttccattaa
    14581 ataagatcca aaggttgtcc tttttcttca aatgcttgcc tgccatttta tatcagttct
    14641 ccttcataaa ttttttcatt gaatccaatt gcaacaagct ggtcctctcg taagatcagt
    14701 ggtcgcttga tcagcatacc atctgtcgat aagacttggc tcgcttttga agccgtaaac
    14761 tcgcctactt gatctttcag tccaagttcc cgatatttca tcccgcttgt gttgaacagg
    14821 cgacggattg gtacttgact cgtttctagc caagtctcaa tcgttgaggc cttcggtact
    14881 tctttgatca tatcaacggt ttgataacta acgccatgct ggtctaacca cgctttggct
    14941 tttttgcatg ttgaacattt cgggtaccag taaaaggtgt acaattttct tacctccttg
    15001 aagatcttgc aggtgcttca ttttggtatc tcatcgataa tatcaacccg ataccgatca
    15061 tgttccctaa aatcgaggat cctccctgac taatgaaagg caatgggatc cctgttaaag
    15121 gaagtaatcc gatatttgct ccaatattct caaagacatg gaacagcagc atcatgatca
    15181 aaccgcttgc gatataggcg taaaattcat tattcgtatc gaaacacacc cgaatcatac
    15241 gatagattaa aataaaatac aagaaaatga cgaatgcacc accgataaag ccgaagtttt
    15301 caccgataac ggtaaaaatc atatcggact cacgaacagg cacatagata ttgcttacgt
    15361 tgaatccttt accaaacaag ccaccggtac caattgccaa aagtccttgg gccggctgat
    15421 aactcattcc tgacgtatca tggaaagggt ccagccatga atcaatacgt gcaaactgat
    15481 aagatttgaa tccaacttta taaagcaagt ctcttccgcc ttcagtcgta accaagaaga
    15541 tcgttcctgc tccaacgact actgccaaga ttactacagg tgcgatgatg cgccaagaga
    15601 ttccagacat caggaaaatc ccgccaaaga tcgcaagaaa gacaagcatc gttccaaagt
    15661 catcttgggc catgatcaat gctaaaaccg gtattgtcac gatcaagagc ttcccaatca
    15721 gccaaaagtc cgatttcatc gtccgttctt ttacttgcgt attatgttta gttacgatca
    15781 gtgccatcat caagatgtac gcaatcttca tcaactctgc tggctggaaa gtaaacgcac
    15841 cgaatcgaaa ccagttttta gaccctgttg aagcagctaa attctgatca tagaatttga
    15901 gcaagagtcc cattaccact aaccccaaac cataaaggta aggagtcagt ttccagatcc
    15961 atttggaatt caaatgcatg atgacaacga ttgctgccgc tccaactaaa taccagacaa
    16021 cttgcatccc tacgcctttc aagactgctg gattgttcgg atcatggcta agtgctacat
    16081 ataatgagag cagaccaatc aaacataaca gaaagactgg taaaatcacg ccataatcaa
    16141 tacgattatc gttcgaagct tttaattttt tttccatgtt tcatcccttt tcttaaaaac
    16201 catactgctg attattatag cggtccagtt agataatgga aagtctttcc atagacattt
    16261 gggaaaaatt aactttccgt ttgtgcagtt gcttgagcaa atgcagtaca aaagcctact
    16321 gcttatcttt agtgtatcat agaacttttt ttgatacatg caattatcta ccagctgaga
    16381 ttctcctcgc tgtgagaagt ttctgtgctt tcattgtatc gaaggatctc ttcggcaatc
    16441 tctagcagct gatcagaaac cagattggtt cgttggatga aataaaaata tcgatggaaa
    16501 ttcaacggag tcattggcac attttctgga acagagcgct tagacaaaat cgagcgaccg
    16561 attccttcct tcagcaacgc aacaattaca tcgttacttt tcacgatcat cttcttatct
    16621 tggatattca tctcttcaaa ataacgctgc gtgtaatgaa aaacaccaga gtttgcttct
    16681 cttacaagcc acaatttgct ttttgggtcc ccagcgatga ctagctggtc ttccatcaaa
    16741 gagtatcttt gtacgccgga agtctctaat ggcttttcga taaaaccaaa atcggattca
    16801 tgtttcgcta cttgttctaa tgattcatga gaattcgtga tttcgatctc aaaatgcact
    16861 ttaggaaacc ttttgatcag atgtttcatc aactgaggca aaagatagac agcaaaggtg
    16921 tgggaagcaa cgatccgaca tacttcttct tcattttgtt cattgagcaa tttttgctga
    16981 gtttcttccc attcttctaa caaaaccagt gtccgttcat ataataagtc ggcttgctta
    17041 gttggaagca tctcttttct tccgttacgt tgaaaaagat cgatattcaa ttcccgctcc
    17101 aactgtttga tttgattcga gactgctggt tgagataaaa acagtacttc tgctgctctg
    17161 gaaaaatttc ttgtttcata tactgctcga aaagtcgtta ataacttaaa catggcacca
    17221 cttccataac tttttatgat aattactata aaaactattt gatttaaata tacaaaaaac
    17281 catctataat agaaatgtac aacaaaacag ttgctgatcc aactagtaac ttagatgaag
    17341 acaatcagct gtctgcttgc tcgtaatcat acattcctat tcattttaca atgtattcgt
    17401 tcatcttggt actaaaatct attaaataaa aaggagaata ttatatatga agaacttttt
    17461 gaaaattatt ccgatcccga tctgcgggtt gatccttggg ttagcttccc ttggaaatct
    17521 gtttaaagac tatcaccatg ttgctttagg caatctaact ggcggtatct ctatgattct
    17581 tatgatattc atttttgtta aattgtttat cttattcgaa cacacgaaac aaacgttgca
    17641 tgatccaatc atcgcctccg tttcaccaac ttttacgatg tcactcatgg ttatttgtac
    17701 ttattttgtt tcatatgaaa gtattgcacc aattgtcaaa ttcatttggc tgtgcgctgt
    17761 tatcttccaa gtgatccttg tcttttattt caattatcac catgttatca aagctgatct
    17821 ttcaattgaa gcaatttatc caagctggtt tatcatttac gttggatttg gggtcatcac
    17881 tgtaactgcc gggaacttct tcccacttat tggaaaaatt ttcttttgga tttcattgat
    17941 ctgctatgcg ttcttattgc cgatcatcat caaacgtgtc tttttcgtaa gaaatatggc
    18001 tcatccgaca cgtccgctga ttacgatcgt tgcagcacct ggttctcttt gcctaacagg
    18061 ttatcttaaa aactttgctg aacctaattt ttatcttgtc gtagcattgt tcgctttatc
    18121 tcaagtattg tatttcgttg tgctgtctat gcttccacgt ttgttcaagc ttcgttttta
    18181 tccaagctat gcagccttta cattcccgct tgttatctct gcaacagcta tatttactac
    18241 tgttcattac ttcaacagtt taggtatcta tagtagcgta ttggatttct taaaagttgc
    18301 ggaatgtctg tttgcaagtg tagttgtctt ctacgttctt gctcattacg tacgtttctt
    18361 aattaaagaa catagcaaac atggaaaatt tgtttctaaa aaaatcgctt aataatcgat
    18421 tcaataggtt gtgaaaaagc tgttctgcat caagtaataa gaacggccaa acaaaaacag
    18481 ctgttcatgt ttttgtactg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
    18541 ttaccaagag gtcgtaaaag aagtggtcag cttcaagaaa taagaaggaa tttacgaaaa
    18601 ttgcttctca aatttttgtg aaatttcggc ttatttccgt agaagctact tctggaacac
    18661 cgtttattcg aaacaagagg tcgtacaaga cttttgtcac gacctcttgt tcttttgtca
    18721 aaatactggt ttagtgctac tatgttcgag tatgctttta gtatttattg acgaaaggaa
    18781 ccgatcaaat gaaatcattg tcacatccaa taaaatctgt aaaggattca gcggtccgcg
    18841 aaatcttacc cggtttactg ctttcttttg ccatcgctgc cttcagcagg cttcttgcta
    18901 tttatctgcc acagttaggt ggagcgacta ttgctatttt attgggtatt ttattaggaa
    18961 atactttttt taaacagcct gttctagcaa tcggcacaaa atttgctgaa agcaaactat
    19021 tagaattctc tgttttttta ctaggtgcaa ctgtcacagc tcaaacgatt gctcaaattg
    19081 gtgtaaaagg aattgttttt gtgttactgc aaatgtcagt cactatcatc ggtgcttact
    19141 tgattggtaa aaaactgcaa ttcagcgata cttcttcttt gatgatggct ggaggaaacg
    19201 ctgtttgtgg ttcttctgct attgcttcaa tcgccccttc catccatgcg aaagaggaag
    19261 aaaaaggaca gatcatcaca ctagtcaatc tactgggtac tgtgatgatg ttgactctcc
    19321 cttttcttgg attacagttg tttggtggaa atctcattga aaaaagtgca cttttgggtg
    19381 gtacactcca atctgttggt caagtagtcg ctggggcaag cttacttgac aattctgttg
    19441 tcgaattcgc catgctattt aagattctac gtattatgat gctcgttgtt gttgtctatc
    19501 tgtttgaaaa attcatgcac aagaaacaac cagctctgtt tgaaacgcca actgatacgc
    19561 agaaaaaacg aaaattaccg cttccttggt atgtattagg gtttgtgatc ttttgtttgg
    19621 tcaacagctt gttttcttta cctgtagtta ttgaccaatc ggcacatttt atcagcagct
    19681 ggtttgagat cactgcttta gcggcaattg gtctgcgttt agactttgca aaattcatga
    19741 aagaaggtac acgcttctta tcttatggat tagctgtcgg gcttctccaa acagtgatgg
    19801 ctttactatt tatttggctg atcaaagttt agaatgaaca tgaaaaaaca gttgtgattc
    19861 tcgcatggaa caatgtccga atcacaactg tttttaattt tgcagatatc cttctaacaa
    19921 caacaattgg cgtttgattt caaccccatc tattgcgttt cccatatatc ctgtcagttt
    19981 tccattttgc ccgatcactc gatgacaagg aataacgatt ggcaatggat tggttcgatt
    20041 tgcctgccca atcgctcgca cagctttcgg actatcaaca gcaacggcta cttctttata
    20101 cgttcggatt tctccataag gaatggtcga caaagctttc cagacatttt gctgaaaagg
    20161 cgtacctctc tttatagata aaggaacatc aaattctttt ctttttcccg caaaatattc
    20221 ttcaaactgt tcttgtgtgc gcgttaaaat tcgctgattc gctgattcgc tttctgaaat
    20281 tggctgaaaa gatacagcag tcaatccgga ttcatctgca tctaaccata aaaagccgat
    20341 aggtgtgtgt attttcattt tgtttgctcc tagtcaatag actggaactt cctccttttt
    20401 ctctatccaa aactacttgc caactaataa aaattgttct taaaagtttg ataaaccgag
    20461 gaaacatggt aaactaaata cgttagttta caaaaagaat acctgttctt ttacgtaaaa
    20521 tcaaatttta aaggtgttat ttaaggttta ttaaattttg aatgtaaacc atatataaac
    20581 atacgattga aaggatagaa acgatgaaag tatcaattgt tgtcccatgt tttaatgaag
    20641 aaaaaactgt tcctcttttt tttgatgagg ttgaaaaatt caaaggtgcc catgagttcg
    20701 aatatttgtt tattgacgac ggatcaaaag acggtacgtt agcagcaatc aagaagctag
    20761 cctcagctta tccttatgtc cgctttgtct cattttccag aaattttggg aaagaagcgg
    20821 ctctgtacgc tggattgcaa gcttctacag gagatttagt gaccgtgatg gatgttgatc
    20881 tgcaagatcc gcccgaactg cttcctgaga tgatcaaccg gatcactact tctgatatcg
    20941 attgtgtcgg cacccggcgt tcgactcgtg aaggcgagcc tgcgatacgc agtttctttg
    21001 cgaaaaaatt ctaccagttg atcaataaaa tcagcgatac agaaatggtc gatggcgctc
    21061 gtgatttccg cttgatgact cgtcaaatgg tggatgccgt cctagaatta acagaataca
    21121 accgattttc aaaaggatta tttagctggg tcggatttaa aacagaatat atttcttatg
    21181 aaaaccgcga acgtgtggct ggcgaaacat cttggtcctt ctggaaatta ttcaactact
    21241 cgattgatgg tatcgtgaac ttctcggatg caccgttaac gattgcttca tttatcggcg
    21301 ctttgtcttg tgtcggttct ggtattgcaa ttttgttcat tattttgcgg gcattgttgt
    21361 ttggtgatcc tacatcagga tggccttcca tggtctcgat cttcctgttc attggtggaa
    21421 tccaattgtt atgtcttgga atcatcggga aatatattgg gaaaatcttc ttagaaacga
    21481 aacagcgtcc tgtttatatc ataagagaaa cagaaaaagt gaagccaaat gaattgaagc
    21541 ctcaccaata atatacgtca aatgaagtaa acattttttt aaatgaaaaa ggactgtgac
    21601 aaaagtcttg tcacagtcct tttttccgaa taaatggtgt tccagaagta gcttcttcgg
    21661 aaataagccg aaatttcaca aaaatttgag aagcaatttt cgtaaattcc ttcttatttc
    21721 ttgaagcaga ccacttctat cacaacctct ttcatatctt attacttctt cattgctgct
    21781 tttagtgctg ctgctaatgg gctttcttct ggttcttctg cttgtgcata ttttttcagc
    21841 aagcgtcgtt cttcatgttt ggtcattttc ttcttctgct cttttttatc aagcaatttt
    21901 tctgtcgttc catcgtattt acaacggaaa taagcaccgt ttttaccttc gatgatttcc
    21961 attttccggt gacattgcgg gcatcgatga ttagagactt ttggatcttt tcttctgcgg
    22021 tagttgcaat ctgaattggt gcagacatag attttgccgt cacgtgtgtt tttctcgcgc
    22081 agattggccc cacattctgg gcatttcttt tgggtaatcg agaaatcttg atattcttgt
    22141 ttgcttgatt tgatctcatt caccaagcgt tttgtttctt tttcgattcc attcaagaag
    22201 gttcctgctt gtttctttcc tgcagcaata gcttcaaggt ctttttccca gctttctgtc
    22261 aaatctggtg ttactaaaga gggatttacc aatactaata attgcttacc tttaggagat
    22321 acttgcagtc cttgaggggt acgttccatc agttcagatt tgattagttt ctcgataatc
    22381 tctgcacgcg tagctggggt tcccagactg tatttttcca tttgaccaag caagcttgct
    22441 tctgacaatg gtttaggcgg tgcagatagt tcttttttga tggcgaaatc gggattgatt
    22501 ctcattcctt tttcccattt gactgtttcc gtctcagttt cagtttcgcc tttccatcct
    22561 gcttggacga cacgattttg ccgaaaaatg aatcgttctt taccaaattc aaccgtaact
    22621 tttgtttggc tgactttatg gggttgagca aacagtccta aaaaacgaga gacaatcatt
    22681 tgatagattt ttcgctcttc attacttaat ttttcataac gtgctctgtt ttcagttggc
    22741 aaaagtgcat ggtgatcggt cactttactg tcttggaata cttttgtctg ttggacgatg
    22801 gctccgtttt tcaaatactg cttgacctca ggagcaaagt cgctgactgc ttgcaatcgt
    22861 tctttcatcg tacttttcat gtcatttgtc agatatttgc tgtctgtacg cggataagta
    22921 acgatcttat gggtctcata caaactttgt acaagactta atgttttctt tgcagaatag
    22981 ccatagcgtt gattggcttc tctttggatc tctgttaaat cataaggcaa tggcgcagat
    23041 tccgttttta atttttcttg gacttctgtg acagttcctt tgcttgtact gatcaattga
    23101 acaagttttt gtgcttcttc ttgtgttttt aaagcaaatt gatttttttg aagcattttg
    23161 gcttgttctt ttccagcttg caacgtgatc gtgaaatacg tttgtggacg gaactgctcg
    23221 attttctttt cttggtctct cactaatgct agtgtcggtg tctgcacacg gcctgctgat
    23281 aaattatccg cgtacttcac agttaaggct ctggtcacat tcaatcctac cagccagtcc
    23341 gcttttgccc gggcaagagc agattcatac aatgcatcat attctttcgc aggtcttagt
    23401 tgcttgaatc cagttttgat tgctttatct gtttgagaag agatccataa ccgcttcact
    23461 ggtttattga aatgcacata ctctaagatc cagcgagcaa caagttcccc ttctcttccg
    23521 gcatctgtcg caatgactgc ttctgagaca tcttttcgct gagccagctg cttgattgct
    23581 tttaattgat gtcctgtttt aggaagtggt ttgattccta gttttttagg gatcattggc
    23641 aacgtgtcca tttgccagct ctgccattcc ttattcagat cttccggcat tttcaatcct
    23701 aataaatgac caagggccca agtaacgatc accttgggtc cttcataata atttttgtgc
    23761 ttttgattag caccaagcac cttgcttagg tctttcgcaa cgcttggttt ttccgctaga
    23821 attagttgtt tcataaaatt cctttctgct ttctactgtg atgggctgat gatttttcaa
    23881 caacgcaagt cccttgtatt gctgtaaatc atcgtcacac tcttcacacc agcgctcatc
    23941 tccgtcattc caaaaggcgc tgagaaagtt actttttgca ttgatatatt cataattttg
    24001 atggatttcg ttccattgtt tttcgtacgc ttgtcgtgct tcttcgaaag actcgaatgt
    24061 ttcttcttct tcaatatctt cttcccagtc ttcaaaaaac caccaaggct cattgtcccc
    24121 gaacatggta attacttgat acatactcgt gctccttctt tcagcttttc tagactcatc
    24181 ttagtcactt ttcaagaaag ttgcaagaat tttctcttat aaaagggtca tttaatgaac
    24241 atcttaaaat tgttacaaat gaatctgatt gtttaaggca aagtagcgat aaagttattg
    24301 atcatccaaa aatagctgtc ttctggatcg attgttggtt cttttgtaaa gtatcctaaa
    24361 aaacgtaagg aaagataacc gtggatcata ctacgaagtt ccctgcttcg atgaactttt
    24421 tcttctttcg ttaattgaaa agcctcaaat atctgcaaaa tgatctgatt cgtttcatgg
    24481 attccatcta ataaaacagc ttcattggta tgcggtacac tgatcagtaa ctcatacact
    24541 gcttgatttg aaaaagcaaa ggatcgataa gtttctgcat agatctgcag tgcttcctga
    24601 ccactttttc ccacaagagc ccgtcgcagc tggtcattaa gttcctgcag aagtttcgct
    24661 gttagcgctg ttttgacatc acgaatattt ttgaaatggt tatacagcga gggagccttg
    24721 atatccaagc gtttggctaa atgctgaaag gttatttgct gaacatccat ctcttctgct
    24781 agttctcgaa agcagtcgat gattttctct tgagacagat ttcttttttt catttccatg
    24841 tttcctctca taaaataaat ccttcactct ttcagtttac cacgaaaaag aaagagaaaa
    24901 aagagacttt cattgataaa ctacatattg tagtttataa taaagagctg tagatgaaaa
    24961 ttttcagaaa gaaggagtct aattggctgc aagaataaag gaaaaattat ttaggaaaag
    25021 agaaacttgg gaacaaaact tgtttgtctt atggtttgga acatttatgg ctggtatcgg
    25081 atttagttta gtcatgcctt ttatgtcact ttacatagat acgttaggta attatagtac
    25141 gtcacaattg aacttttgga gcggattgac tttttcatct actttcttgg tgactacgct
    25201 tatctctcct tggtggggaa aattagccga ccaaaaagga agaaaattga tgctgttgcg
    25261 tgcttcatta ggaatggcaa tcgtcatttc tttgatgggt tgcgtgacta gtgtgtatca
    25321 attagtcgca ttgcgtctgc ttcaagggat tttttctggt tatatcagta atgccactgc
    25381 ccttgtcgca actgggacgc caaaagaaaa aagtggtcaa gttcttggaa ccttagcaac
    25441 tggttctgtt acaggaacat tacttggacc gcttcttggc gggatctctg cttcagcgtt
    25501 tggctatcgc cctacctttt tcattacagg gatcatctta tttttcgtct tcttgttgag
    25561 tctgtttttc gtccatgaaa aatttgtccc agtgaaaaaa gaaaacatgg tttctgccaa
    25621 acagattttc aaagacttga aatatccaca cgtcgtcatc ggtatgttcg tcacaacgat
    25681 gatcatccaa gcatccaaca attccattag tccaatcatc agtctgtaca ttcgccaatt
    25741 gctgcatgga catgggaatg ttacgttgat cagcggaatc attgcttcga tacctgggat
    25801 tgccactttg atagccgctc caagatttgg tcgcttaggt gacaagattg ggagtgaaag
    25861 aatacttgcg attggtttgg gttttgcgat tctggtttat atcccaatgg cattcgtcac
    25921 gaatgtttgg caattagctg ccttacgttt tttgattggt atctctgatg cttgtctgct
    25981 ccctgctgtc caagcattga tcacaaaata ttcgcctcat caagctgctg gacggatctt
    26041 cagttataac caatctttcc aagcaactgg taacgtagta gggcctatga tcggctccag
    26101 tgtttccagt gcatttggtt atcgcggcgt ctttctttcg acttctcttc ttgttttggt
    26161 caacttcttg cttgtacgac acaatacaca agagatccat cagcaagaaa ctgcagaaga
    26221 aaaagcttca ggagtctcca tagcttcagg gcactctcct tctcatagac aataagcgta
    26281 aatgaaaaac actgcttgaa cagttgtcac agactgtcct tgcagtgttt ttgtttaggc
    26341 ctgttttttc aagaactcag ataagaggaa taatcccttt tccaaggttt cttttttaca
    26401 acagtaacct agtcgggcat gcttcggtaa atcaaaggca cttcctggta ctaatagtac
    26461 tcctgtttct tctaataaat tgatacaaaa tgtctggtca tcttcttcga tggctaattt
    26521 aatgaaagaa gtagagacgt aattgggagc gacaagttca actttcggtt cattggctac
    26581 ccattgtttt aaaatcacaa ggttctctgt aatgatcttt ttgtttcgcg ccaatatttt
    26641 ttctttattc ttcaatgcat gtacagcaag atcatctgac aggactccgc cgcaaatcat
    26701 cgtgtagtct cgatactttc gaaaaacttc tgccaactca ggtccagtag ctgtccagcc
    26761 aatacgaata cctggaatgg agtaggtttt ggatagggaa ttggttgcta tacctttttc
    26821 atagacatct acgatcgaca agaattcacc tttatccgtt aaaggcgcat atacttcatc
    26881 tatcaataca tatgcatcta ctgtacgagc aatttctgcg attttttcta aagttttccg
    26941 atctagcaat gtgcctgtag gattgtttgc actattcaaa cagatgattt ttgtttcagg
    27001 tttgactaag gcttctagct gttctaaatc aaactgccaa tcttcttctt ccttcaaatg
    27061 aacaaagtca acagcggcac ctaaagattt ggggatgtca tataattgct gataagtagg
    27121 aagcatcgaa acaacatggt ctcctgcttc cactaacgcg tatagagcta aaagattagc
    27181 accagtcgct ccattcgttt gtaagatgtt ttccggatcg acggtttgat aaagagaagc
    27241 gacttcttgt ttgaaaacat ctgatccttc gatccagcca tagtccaatg gttcgttttt
    27301 gcgtttttcg aaaaatttac ttacgctagt cccatctaag ccaacgagtt cgtctaatgt
    27361 caatgcttcg atcgagcttt gtgcaatatc atataccgct ttcttttccc atttatttag
    27421 ccattcttct acaccgaaat ttgcgatttt catgtttctt tcccccttat atcttgatcc
    27481 agcctacagc aagcaacccg attgaaagca gtgcggccac acaaatcagc ccaatcatta
    27541 ctttttcttt tcccgtaatt tgatgtttgg tttcacgaca agcgatccag tagaagcaaa
    27601 aaccaggaag ataggcaata ctacataaaa ggacataaga aaatcctgct aaaatgatgc
    27661 ccatcaattc aaaaactaca gcaatcccac cgatcactac ttgttttttg attccttctt
    27721 ttaaagaaaa ctggacttga tagatcccga caagtagata acaaatcaaa attgccgctg
    27781 tacataaaga ataggcaaat tgataggcat aatcagtaac taagaaagtc aataagaata
    27841 gattagtcaa gattgcagta acaactaacg aataagttgg ggcatttttt ttattcgttt
    27901 ttccccaacc tttcggcaac agaccgtctc gagccatcaa catcgttgtt tctgctggga
    27961 gcatcgtcca agacaaccaa gaaccaatga tcgaaataat caagccgata ttgatcagcg
    28021 ctgctcccca atttcccacg attcctttta aaatatttgc catagcaggt tgtgaaatag
    28081 ttgctaactc ttcttgagat aagacgccgt atggcaagat cgaagcaagg atatagataa
    28141 acaataaaca aagcagacca atgatcgatg cttgctgggc ttctgaacgt ttcttcgcgc
    28201 ggttggctaa aacacttgcg ccttcgatcc ctacaaagac ccacatcata accatcaaac
    28261 aacctttaat ctgcaaccag atcgttcctt gctgccctgt tccattcact agattatcag
    28321 atacgttacc ccaaaaatca gcagtgaata tattcacttt aaatgcgata aatacaaata
    28381 ttaaaaatag gaataatggc actaatttac agatcgtcac gattgtattg atgacgctcg
    28441 ctccttcaac tccattgttt actaataaag tcagacccca aataaaaata cttgcaaata
    28501 aaatagaagg aacattttgt ccacctttga atatcgggaa aaattctccc atggcgctca
    28561 tcagcatcgt tgcaaaagca acatttccca gccacgcaga caaccagtaa ccccaaccac
    28621 tgatgaagcc acctaattta ccaaatgaag cttccgcgta accgaaaata ccaccatcta
    28681 gatctggacg cttttctcct aaatgattca gcgataatac caaagctaaa ataccaatcc
    28741 caacgattat ccatgaaagg atcgctggcc ctggagctgc agaactagcc agatcacttg
    28801 tgatcccaaa cacaccgctt ccgattgacg aactgacaac cagagcaacc agtccccatt
    28861 tgctaatacc cgtcgatttc ttttccattt ccatcaccct cacttataaa attgaacaaa
    28921 tcacttgcaa aagattccct tctattttgc aactgctctt gcttaacaaa aagcaacttt
    28981 attcatatta ttggatatta tgaacttttt acacaagtat ttcaagtata aaatgaataa
    29041 aaattaattt tattgcaaaa aaacagctat gctatccaaa agaaaaggtg tttcttttgt
    29101 tagccatagc tgttttgatt catttaatat cgcttaaacg tattgtaaaa cttttgtttc
    29161 ttgatatgcg tggtcgatca aaccaccacc cagacattcc attccatcat aaaatacaac
    29221 tgcttgtcca ggagtgattg cacgtacagg ttcgtcgaat acgacttctg cacgattatc
    29281 atccaataag cgaactgtca caggaacgtc ttgctgacga taacggaatt tcgctgtaca
    29341 cttgaattct tttggcattg gttcattcgt cgtaaagtgg atttcacttg catccaagct
    29401 tgtggcatac aatgcaggat gatggaaccc ttgccccaca tataatgtgt ttgtcgctag
    29461 atcttttcct acaacaaacc agggttcttg cgaatctcct ccgccaccga tccctagtcc
    29521 ttgacgttga ccaatcgtat aatacatcaa gccagcatgt tgtcctttga cttcaccatc
    29581 aagagtgacc atatttcctt tttttgctgg cagatagttg ctcaaaaatt gtttgaagtt
    29641 cttttcacca ataaagcaga ttcccgttga atcttttttc ttagcagtcg caaggcctgc
    29701 gcgttcagca attgcccgta cttctgattt ttccatgccg cctagaggga acatcgtttt
    29761 tgaaagctgt tcttgtgata attggctaag gaagtaagtt tgatctttat tattgtctac
    29821 tccacgaagc atgtggacca cgccgttttc atcgcgagtg acttgcgcat aatgtccagt
    29881 agctacataa tcggcgccaa gatccattgc ataatccaag aatgctttga atttgatttc
    29941 tttgttgcac atcacgtctg ggttaggtgt acgtcctgcg cggtattcgg ctaggaaata
    30001 ttcaaataca cgatcccaat attctttttc aaaattcaca gagtaataag gaatcccgat
    30061 ttgagcagct actttcgcta catccttata atcttccgtg gccgtgcata caccgttttc
    30121 gtccgtgtca tcccaatttt tcatgaatat tccgatcacg tcgtatcctt gttcctttaa
    30181 caaaagtgct gttacagatg agtcgacacc gccgctcatc cctacgacaa cgcgtgtttt
    30241 gctgttgtct gtcatgatat caccatcatt tcaattagat tctgaaacat ctacgagttg
    30301 aaggtcgcaa cttttcagta ttttacatta tacgtcatat ttctgaaaat ttcatccctt
    30361 aattatcaag tcgtaaaaat actgttctac tggacctatt cagtacacaa acatgatagt
    30421 caaccaaaaa atcactcatc ttttgagcat tcgatataaa aaattggcat actcataaaa
    30481 agggccagcc tttcggcaat aaggaataat tttataaatt gtgaaaaact atttttcaaa
    30541 attgcttctt atcgcttaaa gctgcacgtc tcatttacaa ccttttcttt ttagatgtta
    30601 tgagttgctc agataagtga ttttgtttac tctaagaaaa aagggaggca aaagatacat
    30661 gaattacttt atcagtgata tgcacttttt ccatgaacgc ttattaggaa aaaacgactt
    30721 tgctccgcgg ctctttgcaa atgtggatga aatgaaccaa cagttgatca ctagctggaa
    30781 tgcagtaata aaagaaaccg accatgttta tcatctaggc gaccttgcta tgcatccgca
    30841 atatgaaaaa gcaaatgcag aaattttgga actggtcact caactgcaag gaacaatcca
    30901 cttgatcaaa gggaaccatg acagtcgcgc ttttttccgt tatttagaaa aaaatgatcc
    30961 taggcttact gacggaagtc ccaaatttta tttttatgat gtaggtgcca tcgttaaatt
    31021 cgaccatcaa caatattact tgactcatta tccgatgttg ctcgggccta acgacactat
    31081 ccgcaatctt catggacata tccatcatta cagcgtacct atcgagaacg atgtcaatgt
    31141 aggcgtagac gcaccggaaa gagaactctt aaaggaaaaa ctaccatttg gcactccgtt
    31201 atctgagcaa caaatcaaag aaatctatga agcaaagagg aagtacgata ggtcgtgaaa
    31261 aaactgccct atatcaagta ttaaacaaat aaagataatt actcatattt ttcatttgag
    31321 taggcttatt gccacaggaa cattaggatc agaaatggag ttgtaacaag acttctgtca
    31381 caactccatt ccatactatt cgtctttttg ctcatggtgt tcttttacca gttttttctg
    31441 taattcttta tcataatttc gggcagctga cgttaaaaat aaaacgattc cgcataataa
    31501 aatcccgatt ccggaaaaca agaacatttt ttctacccct aaacgatcag ctaatggtcc
    31561 tgcaaataat aatccaattg gacctgccag gttcatcaaa gagttgaagt tccctaagac
    31621 acgacctaaa acattcgatt cataactctg ctggatcatt gccatcagca gtgtattgaa
    31681 atatggcgtt gcacaacctg ctcctgcatt caagataaga aagtacaaga acccttgact
    31741 atcgtttgga accaatccgc ttgcaccgat cgtcaggccc ataatgatgt aggctataat
    31801 aataggtttc attcggtctt tccatgttcc tataaaactg atcagtgcac cgcctaagag
    31861 cattccgact gcgtagacta cttccactgc tcccgcctgt ccaacggaac caccaaagta
    31921 atctagcgtc atcaatggat acagactgat tgctggcatg aacaacagca tcacaaatgc
    31981 accattgatc gttatgtacc ataacccttt attatccatc aattgctgca gcccaaattt
    32041 tgcatcttta aaaatagtaa gcggaacttc caatatttca ggagaaactt ttgggatctt
    32101 cacaaaaatc aataagccga caccaaaaac tgcccccaat acatctaata aaataaggta
    32161 attgacagga acgactgaaa acaaagccgc acctagagct ggcgcaatga tgaaattcgc
    32221 tgattggacc attcctaact gtccattcgt ccttgttatg tgagaagatg gaacgattgt
    32281 cggcataatc gactggatcg ttggcatttg gaatgtttgt gcgaccgaac gcataaacaa
    32341 agacacaaaa actagccaaa tcgggaagga ttctgaaatc gttccgacta ctgctaaaat
    32401 aagcgcgaaa atcgctacaa taatatccgt gacaatcaaa agagcttttt tatcccagcg
    32461 atccactagc ggtcctacaa atgggcttag taaaaccatg ggaatcattc ctagtaaagt
    32521 agcaaaactc aaaattgtcg ccgaaccggt ttctcttgtc aaataccaga tgatcgcata
    32581 ttggacgacc atactagtga ttcctgacaa aaactgtccc gataaaaaga acaaataatt
    32641 tctcttccaa ttcgttaaaa cgaccgactg ttcattttcc ataaacatcc ctcttttctt
    32701 tttcattttg tatagcgttt gcacaacaat cattatacgg gattcttttt tcaaaaaata
    32761 cctttttccc taaataacaa tcgtttcaat ttaaatttct ttacttagaa acaataaatc
    32821 agcaaaatat taaaaataac aacatgtgtt aaatttaaaa ataaaaaaga caactaattt
    32881 gcgtcgtcct tacaaagtta gtcatctaac aataggccgt ttcaaaacta gttgtctttt
    32941 tatatccaca gatcattcga ctttttcgaa taacccacgg ctaatcatcc catccatcag
    33001 aaaataaaat ttatcttggc tcattttctg ttttggatct ttgaaaatat tgactgcttt
    33061 caatccattt gctgtagtga tcgacatttt caatgtttga atatcttttg atacagtgat
    33121 ttttaatttg aatccttctt tgctttgcgg cgtggcatct aatggtcgga tcaattgata
    33181 attgcctccg tttgtttctg taaatccatt atcgcgcaat gtatatcttt ttgcatcagg
    33241 atgcaaacga tagctaattg gtgcgcccag taccgttgct gttgtttcaa atgccatgat
    33301 tttcacctcg ataaaattag tttctcttta ttataaaagt ttatcctgat ttttactagg
    33361 tttttcagtt aagttcccta aaaaatgaag gcgttctctc agacatctgt tcatataaga
    33421 atgacttcac tagttatttt ttgacagcac gaactaattg atcaaggaaa tactggatgt
    33481 cgtcttcggt atttcccaaa ccaaagctga tacggatgga ttcttttagt atcggtgtat
    33541 cttctccata cattgcttct aatacgtgtg aaggctcgat atttcctgct gtacaagctg
    33601 aaccagtaga aatagcgacc ccattaagat caagcttcat cagtagcaaa tcatttgctt
    33661 cgccaattaa gcgaagattc aacacatgag ggagtttatt cgttaaatca ccatttactt
    33721 gataagggat ttcgtttgct tctaatgtat caagaatcaa ttgctggaat cctttatatt
    33781 tgttgttacg tgcttcacgt tcttcaggtg acagcagttc aactgctttt gccatacccc
    33841 agatccctgc taaattttct gtacctgcac gccgcttttc ttcctgctcc ccgcctaaaa
    33901 gcaatgcagg gatatttact ccgtctcgtt tatataagaa accgacgcct tttggcccat
    33961 tgattttatg agcagagata cttaataaat cgattcctaa ttcttctgga tggatcgttt
    34021 ggtttccgta cgcttgtacc gcatctgtat ggaaataagc tggatgatcc gctaaaagtt
    34081 caccgatttc tttgattggc atcaaatttc cgatttcatt gttcccgtac atcacagaga
    34141 ccaagatcgt atcttctcgt aacgcttttt tgacctcttc gatcgacaga ttcccttttt
    34201 catctacagg cagatatgta acgtcaaacc cctttgtctc taaatatttc atggtattaa
    34261 gaacagcagg atgttcgata gctgtagtga ttagatgtct gccttctttt tgacgcgaga
    34321 acgctgtttc taagatagct gtattatccc cttctgttcc cccactgtta aagatgatct
    34381 cgtgaggttt tgcttgcaga ctgtctgcaa tgatttgtct tgctgtttcc aatttttcat
    34441 gcgcgtggcg accaaatcca tgtatgcttg aaggatttcc aaacgtgtct tgcatgattg
    34501 ccgtcatcgc ttcaattact tgtggatgca tgggggtagt tgccgcatga tccagataaa
    34561 tagttttcac ttcattcatc ccttccaaat tattgctagg cacattctag cataaaatag
    34621 cgaacaaacc aaagaagagc tcatttttgt ctagaggacg tttcatgggc atagaaaaaa
    34681 ggagctgtga caaaagtctt gtcgcagctc cttgaagctg ctcacttctg tcacaacttc
    34741 tttccctctt ttttttgttt attcgaattc tttttcttcc agacggaata acggactcat
    34801 cggtgtattt tcatggatgc gtttgaccgc ttcacccatt aattctgaac atgtaacgat
    34861 tgttaagttt tctggatgac gattttcttc tgtgaaaaca gaatcagtga tacaaatatc
    34921 tttgattggt gcattgtcta aaatactttt cgcatgatcg gacaataatc catgtgacgc
    34981 acaggcatat acttcttttg cgccgttttc ttttaataca tttgctgctc gtgcaagcgt
    35041 tgctcctgtg ttcaagatat catctaccat gatacatgtc tttccttgaa catcaccgat
    35101 cacataacca ccctcagttg aagcatcatc tgcatgatct acgatggcta atgtgctgtt
    35161 caaatattct gaaagactac gtgcacgctg aacaccgctg ttttttggcg aaacaactac
    35221 gatgtcatct cccgtcagac ctaattgacg gtaataatga gcaaacaacg gcatcgtaaa
    35281 taaattatct acaggaatat caaagaatcc ttgtacttga accgtgtgca gatctaaagt
    35341 cagtactcga gttgcccctg cttcttcgat caagttagcg atcaatttcg cagtgatcgg
    35401 ttcatgaggt ttcgccgtgc ggtcttgcct agcataccca tagtaaggca atacgacatt
    35461 gatcgttttc gcacttgctc gcttcattgc atcgatcatg atcagtagtt ccatatagta
    35521 atcattgaca ggtgcattcg ttgattgaac gatatacaca tgatcccctc gaatactttc
    35581 ttcgatattg atggaaatct cgccatcact aaattgcttc acgacgcttt tacctaattc
    35641 tgttccgaac actttcgcga ttttttcagc taatggacgg ttgccattca aactaaaaat
    35701 cttcaacgtg tcatcctgat acttattact catgtagtcc tccccaaaac ttctctattc
    35761 ttcgatagtt taacatattt ttccaagatt ttctgttaat ttcagcaaat tcttaaggaa
    35821 gtgaaactgt tccgttactc caactggcag gaatgacttt atgataatcg ccccagttgt
    35881 cgtgtacttt gtaataacgt tctcctgttt cagtttcaaa ataagcgtac gctgtcaccc
    35941 aatgattgcc atatgtactg ccaagtactt tcaaaatacc aatcatcaca ggtttgcctt
    36001 cacgtatacg ttttgtagct cgctgccatg aacctagaag agttgctcgt gcacgctcat
    36061 gattgccatt cttctttaaa aaactagaaa tccctgtact aacctgaaaa ggaatcgtag
    36121 gaagtcctag tggttggatc tggctacgca atgccttcgt caaaaccaac gaatcagccg
    36181 tatttttctt gcgaatctca tttggaagca tccacccctt acgatagtct tgataatagg
    36241 caaggagaac tgctgccgcg taagtaccac aaagatagcc tgagggggta gcccatttct
    36301 tatacctcaa aaaacgttct tctggcaatc ccacccagca atttgcgagt tcctttcctg
    36361 accttttttc ttcgatcaaa acgggaatat cttcttttct gttgatccat ctagataact
    36421 cgattggctc ttcattgatc agcttccatt ttttacaaga aagaccctct tgataagtat
    36481 attttgctgg agaatattct accggatgtc ctgattgatc gataatcaca taactttgat
    36541 cttcaaaaac cagagctaaa aaaagcaatt tactttttct tgactgcagc tgctgagtgc
    36601 tggctaattt tttaattttt ttatctactt tgaaatataa aggtttatcc actacttgat
    36661 tcagttggga tcgcctcctt tttttcatta taagtgaagg attgaaaaaa acaaatcagt
    36721 ttgattgttc gaattttcaa ttttttatgt tctatttgtt aattccttca ttttgtaaga
    36781 taatgggcac acgaaacaaa aaaggagcga tcgatgaatg aacaaagaac gattgaaaca
    36841 agatttctat gaagcagtca atgaagaatg gcaaaaaaca gcaaaaatcc ctgcagataa
    36901 accagctaca ggcggtttcc aagatttagt tgacgggatc gaccaacttt tgatgggaga
    36961 aacagacgat ctgcttgccc atccagaaaa agtgtcttca gaaaaaatga aacatttcct
    37021 tgcatattat caactggcaa acgattatga ccgaagaaat gagttgcatg ctcaaccgct
    37081 tcaacctata atcgaaaaag ttgaatcatt gcagtctttc gacgaattga ataagcaatt
    37141 tccagaatgg ctgttggata cattaccatt gccatttaat ctggatgtag atgcagatat
    37201 gaagaatgcg caaacgaacg ccttctttgc ttatccgcct tctttattcc ttccagataa
    37261 gacgtattat gcatcagacc atcccaacgg tgcacaacta ataggcgttt tctttgatat
    37321 ggctgttcag ttattagagc ttgccggcaa agaaaaaagt caggcagaag caatcacgga
    37381 acaagcagtg caatttgata aattgattgc tcctcatgta aaaagtgcag aagaaaacgc
    37441 agattacagc aaaatgtata atccgcaatc ttttgataca tttattaaat acacagataa
    37501 actggacttg aaaaaacttg tcacgggttt gattgggaca actccagata aagtgatcgt
    37561 aactgatcca gtgtacttcg atcatttgaa tgacatcctg acagaagctc attttccgtt
    37621 attcaaaagc tggatgatca ttaaaacagt acgttctctt agtggttatc tatcagaaga
    37681 attccgccaa gtcagcggaa tctattctcg cgtattgtct ggaacagacg aagcaatgcc
    37741 tcagaaaaaa gcagcgtatt accttgcctc tggacaattc gatcaagtag ttggcgatta
    37801 ctacggaaaa aaatattttg gcgaagctgc taaaaaagac gtagaacaaa tggtgaaaaa
    37861 aatgatcgcc gtttataaaa aacgtctaga agaaaatgac tggcttgggg atgcaacaaa
    37921 agaaaaagcc gtcataaaat taaataactt aggtatccaa gtaggctacc ccgataagat
    37981 cccagcatta ttcgaccagt tcaaaacagt acctgcagat cagggtggta ctttgctttc
    38041 aaatactctt gttttcagca aactcacatt aaaagatcgt ttcagcaaat ggaacaaacc
    38101 agtggatcgt tcagaatggg aaatgagtgc tgacacggtc aatgcttatt atcatccatt
    38161 tcggaatgtg atcgtctttc cggcagcgat ccttcaagca ccattttaca gtctagaaca
    38221 atcaagctct gctaattatg gtggaatcgg agctgttatc gctcatgaga tttcccatgc
    38281 atttgataat aacggcgctt tatttgacga atatggcaac ttgaacaact ggtggacaga
    38341 agaagatctt acacaatttc aaaacaaagc acaagcaatg atcgaagaat tcgatggtct
    38401 gccttttgct gacggaaaag taaacggaaa attaaccgtt tcggaaaata ttgctgatgc
    38461 aggcggtcta agctgtgcat tggaagcagc aaaaacggaa tcagatcttt ctatcgagga
    38521 attctttgtc aattgggcaa cgatttggcg tacaaaagca aaaaaagaat accaacaact
    38581 tttactgcaa atcgatgtcc atgcccctgc taaactccga gcaaacgtgc aaccaaaaaa
    38641 ccttgacgaa ttctatgaaa catttggtat cacggaacaa gacgctatgt atctagcgcc
    38701 agaaaaaaga gtgcgaatct ggtaagaggt tgtgccagaa gtggtcagct tcaagaaata
    38761 agaaggaatt tgggaaaatc gcttctcaaa tttttgtgac atttcggctt atttttcgaa
    38821 gaagttactt ctggaatacc atgtattaga ggttgtgaaa aagctgctta gtttcaagta
    38881 gtaagaaaaa ttttgtgaaa atggctttcc acatttttgc ataaatttga cttactaccg
    38941 agaaactggc ttttgaacac cgtttatcga gaggttgtga aatcagcgtc ctgacctgag
    39001 tattagagaa aaaaacggaa aaatagttcc tcatattttg ccgtttgttg agctaatacc
    39061 gagggtcagc tgattgaaca ccgtttagtg gagagattgt gaaaaagctg tcctgcatca
    39121 agtaataaga acagccaatc aaaagcagca aggagaacct catctataaa aagatagaag
    39181 gaagtgagac atctattggt ctcatttcct tctattttta ctttcctcag tcaattgttt
    39241 tacatcataa aaggagaaat tccacttatt tttttcgctg tttcatgaca taaacaacta
    39301 caccgattaa caaaattcct ccagctaatg ctagcaacgg gttattaaat tctcccgttt
    39361 ttgggagtcc ttgagtgcca tttttttcac tttcttttgc tgtcctatcc ggatgagaag
    39421 gttttccatt tggttttcca tttggtgatt catttcctgg attttctctt tctggtttgt
    39481 catttcccgg gggcacatcg gcagtttctc gcacagtgat cgctttttgc tgggtaacag
    39541 attctgtatg agcagaccat ttttccccat caaatatttc cgaacgataa gtgacttcta
    39601 gcgtatattc tccttttgaa ggtgctgaaa aaatagcggt atgattagaa aattgagcac
    39661 ttgactgtgt ttcaacgatt tggatctttt ctgggaaata acgcatatct tcaaagacag
    39721 gcgttccttg atttggtgtg aaagttgaca caaaaactgt aaaaggcttc agttcaatga
    39781 tatttccttc tgcattctcc gccacaccat ttgcttcttg tataataatc ggattatttt
    39841 gttcatttgg ttttgtctga tttggttctt gtacgattgc atactctgga tattccgaag
    39901 acggtgaggt ttctccgtca aaaatagcac ctacagataa tggaagacca tccactcttt
    39961 ccttttctgt ttgacgattc cccggtttaa tctgcatcag tccatgaaca tggatacttc
    40021 cattttcata gcgcgtaaca ggctgctgac cttctttcga acatgtattt agagactcga
    40081 tccaatcttt tgatatggta tgtccaagag tattttctga ttgtttctct cgttcattcc
    40141 aatagtagtt caacgagtgg tagatttgat cttcgttttg attcaatggg cggatctgct
    40201 ctctcatctc aagattctct gttcggaaag ataagatacc agaagcatgg tagtctgtgt
    40261 ttcctgctga agaggtataa agagccacat tatatgaacc gttattaaaa cttgtactgc
    40321 tattgacgat aatatctgtt ccgctattgg aatcgattcc tttcgcaaga ttttcgaaag
    40381 acaaactatt gatcaattca tgagggcccg ttaaagaaga accgcccatt ttgaatccat
    40441 taccgttacc ttctccctca attcctggta cccagccgtt tcgataagca actgagtttt
    40501 tgatgattac ttttccgatc gaacctgttt catttttagc aaaaaggtcc caaccgtcat
    40561 ctgcattatg atacgcgata caaccgtcaa atacatttcc ttctcctatt gtcagtttgg
    40621 ccgcaaatcc atcggcatct tcaaatcctg gatcggcatt ttcaaaagat gtacagttct
    40681 tgactaagtt atgcgcgggc catgcttcga aaggatcatt tgagctaccg ctgatttgga
    40741 ttcctgtatt tccattccga taagcatgaa tctcttcgat caaattatga tttccagaaa
    40801 tctgtaatcc tttttgcata gctgcactat ttgtaatgtc aaaccctcgt aaaccccagt
    40861 agtgactgaa cagtgtcatc ccatttccag ttcctttaaa atcaagtaca ggacgcttgt
    40921 tttctggatt tttctctgca atcaactgaa tcgggttttc tcttgtcccg ttaacgcctt
    40981 ttggaatcgt caatcctctt tcgaaggaat aagttccagg cgccatgacg attgtctgcc
    41041 cagcatatgc gtatctcaat gcttgtgtca gagataatgg attttgtctg cttccgtttc
    41101 ctgcatctga cccttctggt gttacataaa ctgtcgaata tttaggttta cggtagtcca
    41161 ctgtatgaag aatctctttt gtttcataac tagtcatctc tacgtattct tctggcggat
    41221 agtttttatc tggcgtaaaa ctgaaagtaa attcgttcgt tccttccatc agttttgtct
    41281 gataatcaaa ttcccgattc gcagtgatag ccaagttttt tgcgttctta agcacctctc
    41341 ctgttgcttt atttttaatt gtcaataatc cgtcagcatt tgctctaaag gacatagtat
    41401 gatcttggtt tcctgaatga ttactagagg tcacagtgta aatcggatca atcaagcgag
    41461 taggtctttc ttgcccagga ggatctgtcg caggatctga gtaagcaact tcgattcctt
    41521 ctaccttgat cgtcatattc cttgctgcag caaatcctgc ataaataacc gattcatctg
    41581 aagcgtataa tttttcccag tcatacatga tgtcagaagc catttcttct cctgtttcag
    41641 gatcgtaata atatgcctct aaaccagtat tcgttttttt catctctacg tccagtttag
    41701 taatcgagtt cgtggccgga aacagattcc catgaccgtt tccgaatata ttataagaac
    41761 ctgtttcttt ttccaagaac cctgctgaag tctctaaagg tgttgtttcc actgagacac
    41821 tcccaggagc tggcgcagct tgcggatctt cagatgatat tcctgtgatt gcccgtgtcc
    41881 ccaatccaag tcgcatcgta tatttgctgc cgctcgtgtt gaccacttct tgtctatccg
    41941 agtcccagac atattcgatc cgactgccta gtatggcaaa tgaatttgaa taaaatgcct
    42001 gcccaaacaa atgatttttt ggtactgcgt ctcttaccat taaagcaaat ccttcttggg
    42061 cattcgtata ggtccaagat tctatttcga tcgtcgcccg catcctaaag ttgatgtctt
    42121 tggggattgc ttgataataa taggtcattc catctgatcc gcttgactgg aattttccgc
    42181 cgttattcgt ggaagagagc cgcacgccgt cttctaatgg ttcaatggca tacctactag
    42241 cacttgtgct ttctccgaaa acaacccctt tccactcttc atttgcttct tcagattgct
    42301 tttttgtctt ttcattttct gattctttca tcataggatc atttccttct tccgtatcca
    42361 tatcttcact agttcttggt atttcctcca ttgttgacat cccttcaatg ttcacgtgtg
    42421 cattttcttc gttcacagac tgttgttcgt tagcagcaac ttcaacgaca gaaattaaaa
    42481 ccggggtact gagcataagg agattcatca ttagcgcact gtattttata cgttttttta
    42541 acaagtgtat tcctcccgta acaaattaga aagcgtttac ttaaagtatt acctaattta
    42601 tttttactgt caataaaaaa caaaattata ataagtgtat tttataaaaa gaaaaaatat
    42661 aacttaagtt gttgataata aagagattta ttaacgtaaa aagaataagt ttttttaaaa
    42721 taacgtataa aaaagagcat aaaacaaagt cgtttgtttt atgctcttct tttattattt
    42781 tgtgcttttt attttatcaa atcactttgt ttttttataa aatttctgcg cctagtgtgg
    42841 actcaaaatg tctcaatgcc cattcatgtc caacgggatc gaaacttgcc acggcatctt
    42901 tgaaaatatg gagtttgtat cctagattat acgcatcgac tgctgtatgc aacacacaga
    42961 tatctgtaca tacgccggtc aaataaatat ctgtgatctg gcgttctcgc aaacggatgt
    43021 ctagatctgt tccactaaaa gcggaataat gccgtttgtc tatccaataa acattttcct
    43081 cttgctcata gtcttggtat aaacttttta aagagccgta caattcccgt ccacccgttc
    43141 ctatcacatt atgcggagga aataagcggt tctccggatg aaaggaatct tttggatcat
    43201 gcgcatcaat ggcaaaaacg acaaaatcct tttgatcaat aaacttcttt gtatgagcag
    43261 tcagcttttt ttcaattact tgtcctgctg tcccagtagt cagctttcca tcctctgcaa
    43321 caaaatcata tgtgtagtca atcgagatca atgcgttcat cttagtcctc cttatacgag
    43381 atgtttgaca aattcgatca ccatttctgc tgatcgtttg cctgcgtctt cgataaattc
    43441 gtcaaagctt tgtgtcgccg agtgatctgc tgtgtcgctc attgcacgta cgatcaaaaa
    43501 tgggatattg aactgacgag ctgtttgtcc aacagcggct ccttccattt cgcaagctaa
    43561 tgcttcaggg aaattcgtta agatttcttt gattttatct ggcgagtcga caaatgtatc
    43621 acctgtaacg atcaatcctt cttttgcgtt caaattcgtt gcttttgctg ctttaaccat
    43681 ttcactgcgc aaatatgtgc ttgcttcata atatagaggc attcctggta attgtcctgg
    43741 gttgtagcca aatcctgttg catctgcatc aaaataagct actttgtctg atataacgat
    43801 gtcacctact tgtaagcctt caccgattcc gccagctgaa cctgtattga tcaccatgtt
    43861 cacgccgtat tgttggatca acagacttgt ggtaatagat gctaagactt ttcctatacc
    43921 ggaacgtacg acgatcactt cgtggttacc taatgaacct gaaataaata aagcgcctgc
    43981 gcgttcccat gataatggtt cgcttaggtt ctctctcaaa atttttactt cttcttccat
    44041 tgcgccgata ataccgattt tcatcttttt aattccttct ttctatataa atgcgattgc
    44101 taaaaaaacc acacataaca ttaggataac aataataaga gatttcatca aaaaagagtt
    44161 ccatttggtc gtcttttccc gttcgctgat ccttgttttc gtgattggtt tattcttttt
    44221 gctttcttta cgataaaagc tggcaatctt tttttcttct tgctgataag ctgtttctgc
    44281 ttttcgctgt ttttttaatg actcgcttgc ttgtgcttgt tgtcgcttgc gaagttcgct
    44341 tcgcgtaata agcggtcctt tggccataag ccgatccctc tactttcctt tggtcaatag
    44401 ttcccagtat tgaatcgcat aaatcgtctt tgcatcacaa atcaactgat ctttcatcgc
    44461 ttgttgcgcc tcttcaagag ttagatgata caactccagt acttcgtctt catcttgtgc
    44521 taaaggattc tcgaccttct ctaccccttg tgcatgataa atatgaagga cttcatttgc
    44581 aaaacctggt gaaagataca tcgaagtaag atggctcagc gacttcgcac gatagccagt
    44641 ttcttcttct aattctctag ctgctgtcat ttcgggattt tgtccttctc ctggatcgat
    44701 ttttccagct ggaatctcca aaatcacttt ttctaaaggt tttcgaaatt gtttgacaag
    44761 aagcagacga tcttgttcgt caaaagcaat gatcccgaca ccgccaggat gaaataccag
    44821 ttctctcttc gaagttcctc catctggcaa acgaacttcg tctacagcaa catcaataat
    44881 ttttccgcta tagatttctt tgcgactgat tgttttttct tcaaattgct cattgttcag
    44941 catatcttct cctgctttct ttgtaatcat aactgctttt ttttcatttt tcaatttttc
    45001 tcaaaaactc cttattagtt taccatagat aaaccttaga ttttctctaa atatagaaaa
    45061 tccttcttta gtccgatact ctttatagta gtccgtatgc taaaataagt atgtaatcga
    45121 ataacgaaaa gaattcaata ctcaacgata aatgacatgg agggtttatt atgataaaac
    45181 gaaattggcc gtggcttcta gcattggtct tgctatttct ctttgtccga aacaatagtt
    45241 tttcttttgt tctaggactt ttattgatag gtgtagtttt agttttgtgg ggactctttg
    45301 gttcgcgcag gaaaaaggga aaacaagaag aaattcctgc actgactgac gaacttgaaa
    45361 ctcactatga aaaaagcggt atgacagcga atgaaatttc attttttcga caaacaatga
    45421 atcaaacaaa aaatgagatc aatcaattgc aaatgaatat gcagcagaca gccaaactta
    45481 aagcaatcga tctccgccat gatccagtca aagcagcgaa agctttattc aaagaattgg
    45541 tgaaagaacc aaaccgactg catgaagcaa gtcagtttct ttatacgcat ctgccgaatc
    45601 tagttgattt gacaaataag tataatgaga tcaatgacca tgagatcaaa aacaaacaga
    45661 cctatgaaaa actagaagaa agcgcgcaga tcatcgacca gctgtcagct ttaatcgcac
    45721 aagattatca aaaattcgtt gctgaggatc tagatgatat cgacgtagag atatctgttg
    45781 cgaaacagag cttaaagcgt gataataaat atgaaccaga acaaaaggat taactctttt
    45841 gttcataaaa gaagactcgg aacattcaga tgatgtctgg ctgtttcgag tactttttct
    45901 aaagtagaga cattcatgta agaatcgaga acagattcaa caaggaggaa ttttttatgg
    45961 atcataaacc gactgaaaca aaagatgtta caccagttaa cgatacactt gaagatttat
    46021 tgaacaaccc gttttcaact ccaacagata cgctgaccac ttcacaacaa gcggagattg
    46081 atcagctcca agagcaacag acagctgctc gtttaattga caaactaccg gcggaacgcc
    46141 aagaacaagc taaacagcta gcagcaaaaa tcgatgctaa tgatgcacag tctgtcatca
    46201 gctacggatc ggctgcccaa gcaaaattaa gtgaattctc acaatcgatg ttgaatcatg
    46261 tgcaagcaca agatattggt cctgtaggag attccttaac tgaactgatg tatcgtctcc
    46321 aagaagccaa tcctgacgaa ctgcgtgccg gagaagggaa catcttccag cgtgtcttcg
    46381 ggaaggtcaa acaatcgatc tatgaagtga cagcaaaata tcaaaagatt ggtgcacaaa
    46441 ttgacaagat ttcagtgaaa ttagataaag aaaaagacgg tctgctaaaa gataatctga
    46501 tgttagaaca gctttaccag aaaaacaaag actactttga tgcactgaat atttatatcg
    46561 ctgctggaga attgaaaatg gaagaacttc aaacaacgat cattccagaa gcgatgaaaa
    46621 gagcagaaga aactggcgat cagatggatg ttcagattgc caatgattac acgcaattcc
    46681 tagatcgtct ggataaacgt acgcatgatc ttcgcttagc tagacagatc acgatccagc
    46741 aagcaccaca aatccgtttg atccaaaaca caaatcaggc cttggctgaa aagatccaag
    46801 catctatcgc tacagccatt ccgctgtgga aaaaccaagt cgtgatcgca ttgaccttgc
    46861 ttcgtcaaaa agatgcagta actgctcaac gccaagtctc tgaaacaaca aacgacttat
    46921 tgaagaaaaa ttcagaaatg ctgaaaatct cagccatcga aacagcgaaa gaaaatgaac
    46981 gtggaatcgt ggatatcgag acccttcaaa ccactcaaaa tgatttgatc gaaacaatcc
    47041 aagagacatt gcgtatccaa aaagaaggaa aagaaaaacg tcgtcatgca gaagtagagc
    47101 ttggacacat ggaagaagat ctgaaacaaa aactgttgga tttaacccaa tagatcacct
    47161 ttcgcttgtt actaaaacaa acgcccaaca acagtttatt acaactgttg atgggcgttt
    47221 ttcttatctt tcttctaact tctccattgg agtctatttc tcattcatct tgctgtgtcc
    47281 atagccatag ccaaaataaa tgatcagtcc aataaccatt acgataccga acacgatcca
    47341 tgtcacagtt tgcagacgaa gcatcaatac aatacaagtc aagatggaga cgattggcaa
    47401 tagcggaacg aatggcactt tgaattctcc agcttttggt ttgccaaaca tttttctcaa
    47461 cttcagtaag cctagactaa caagcatcaa ataggcgagc gccatgatat tcgttagttc
    47521 cgctaataca ttcaatggga aaaatccggc aaagaataca gctgccacac cagcggcata
    47581 agtcgctttt ttcggtgtac gatgcttttg atcgatttct gtcataaact taggaagtaa
    47641 gccatccttg ctgacagcaa acagtaaacg agcaagtgaa tacatcatcg agatcgttac
    47701 cgtcagtagc gttaaaattg cccctacagc gatcaccgcg cccactgttc catgtcctac
    47761 atacctcatt gcaaaagcca ctgggtcatt gactcctagt tcagtatacg gaacgattcc
    47821 tgtcaagatc agcgtcacga tgatgtaaag gacagtagca atcagtatcg aaccaatgat
    47881 tcctcttggc acatcttttt gcgggttttt gacttcctcg gctgccattg caacggcatc
    47941 aaatcccaag aaagcaaaaa acacaagagc tgcaccatct agcacaccgg aaaaaccaaa
    48001 aggcatgaat ggctgccagt tatcaggttt aacgaaaaat attcctacaa tgataaacaa
    48061 cgcgatgata ccaaatttga catataccat ggcattattc aaacgtaatg ctttcttggc
    48121 ttcaagcgac acgatgtaag tgacgaatat cactacgagc accgcaatca agtcaatgta
    48181 tgtaccattt tccggattgt acccgccagt taatgcttgt ggaagttcgg cccctaaact
    48241 tttcaaaaaa ccttgcatat agccagacca tccggaagca acagaggaaa ccgctagtaa
    48301 aaattccccg atcaacaacc accctgtcag ccaggcagca aattcaccaa atacaacata
    48361 cagataggca tatggcccac caatcactgg cactcgcgaa gcaaattctg cgaaacttag
    48421 tccagacaaa atgacaacga ttgcagctaa gacaaatgaa agtgacagtg ctggaccagc
    48481 attttgattg gccgcaaccc ctgtcaccac aaatattccc gtaccgatga tcgcaccaat
    48541 tccaagcatg atcaaatcag acgtttttag ttcttttttc atctcactcg tatgattgag
    48601 attgatctct ttcttacgaa acaaatccat tgaagttcct ccccatttta ggttggaaaa
    48661 cagcgattaa actgttttca tataccctaa aaggatacca cctaaaatga ctaaaaggca
    48721 accaacaata acataaatga gctctttttt cgtttttctt tcgccaagga tgaaaatccc
    48781 acctaaagta gagataatga tccccatttg tgaaagtgag aagccaacag ctaaaccaag
    48841 tgatttgact gttagaagca tgcagatgtt tcctaagccc cataataatc cacagatcat
    48901 gtttttccac acaaactgat cgactttaac tttacggaaa gcaaagaagc ttgcgcctaa
    48961 gatcattccg atactttgag gtaagataat tgacatcgca tccaaattcg cccagttgac
    49021 gatgatggta tatacaccat aaccaatcgt agagaagatc aatgctctaa aacccttacc
    49081 aaaatcaagc attttattat ctgtatctat ttttccttcg tcatcttgtc gggcagtcaa
    49141 atacgcaccg cttacaagaa gagctaaagc aataataccg tatacataat ctttcgtttg
    49201 tgtccattca tggaagaaaa cggctcccgc aatcgtattc aaaattaatt gaaaaccagt
    49261 agacaatggt agccctacag aaacacccat gtacttcatt ccatggaact gtccattttg
    49321 tccaacactc caagctaagc cggataaaaa tccgatcaag aaaatccaag gggtgattac
    49381 aggactaaca acgaaaaaga caactaaaga aaacagaaat gcaccgatcg tcattcctag
    49441 tgtttgttga ttggcatcgc caccaatttt accacttact aaaccaatac taccccatgc
    49501 caacataggg actaatgcta ttaaaatttc cataaccaat ctccttttat ttcacatgat
    49561 aagaaactta tcagcgaaaa cgttaacaac acaagcactt tttttcgttt ctaacgattt
    49621 tctaacgttt ttcattataa aaatttaaga ttgggaaaaa taaagtcatt gttgtccctt
    49681 ttccttcagt tgattcagcc gtcaaatgga tacctaattt ttcggccaga ttttttgcta
    49741 aataaagtcc taatcctgtt gaatgtgttt tgctaagtcg accatttctc ccagtaaatc
    49801 ccttatcaaa gatccgtttc aagtcttccg ctgggatacc gatccctgaa tctttaactg
    49861 aaaggctgac tccttgcgct tccttttcta tgctcacaat gatgtttccg tgatccggcg
    49921 tatatttgat ggcattacta agcaattggt tgaagataaa agcgatccat ttagcatcgg
    49981 tcaaaacagt atgatcctca ccaacgatct cgtagcgaat atttttttgg ataaaataat
    50041 ttgcctgact gcgtatcaca ggctgaatga ttccttttag tccatattct tggatgaggt
    50101 agtcccgcga gaaactgtcg agtcttgcat aatacaaaac ttgttctacg tattcatcga
    50161 tttttgataa ttcattttct agtaatgtat acttttgatc gtcaatatcg aactcgatcg
    50221 aacgaactag caatttagaa gcggaaagtg gcactttgat ttcatggacc catgagtcaa
    50281 tataatcttt ttggtcttgt tgattactga ttgctgattg cattacttct tggtgttcac
    50341 ggatcagctg attgatatat tcttctacca gtttttcttc ttctgtcctc ccaccactca
    50401 aataattttg caaaggggaa gttttttgta tgtcggctaa ttttccccac catcttctct
    50461 tgctcaagta accacccagc agataaaaaa tcagaaagac gccctcaatc aaagctaaat
    50521 aagcaattgt gctcaaatca actttgatgt ttggcgccag ccacatcaca aagatcgtca
    50581 atgcaataaa aaacagccat cccaagatca atgtccactg atctttcaaa tatttaaaaa
    50641 aattcatctt aaccgctcat cctttcaggg aacgatatat ccctgtccta cttttgtttc
    50701 gatatatcct tggatacctg cctgttcgat ttttcggcgt aaccgattga tgttgactgt
    50761 caatgtattg tcatctacaa atcgttcgtc atcccacagt gctcgcagca atttttcccg
    50821 acttaagatc tttccattgt gcttcatcaa aataaataat agacgatatt catttttgct
    50881 caaatcaatg atctctccgt tgatttccat acttccgttg tcaacattca acgtaatgcc
    50941 attgtggctc atcatttcac tagataattc tgtgtagttg taagaacggc gaagtaatgc
    51001 attgattttt gcaatcagta catcgatttg gaaatgtttt gtcacaaaat catctgcccc
    51061 catgttcata gccatgatca tatccatatt cgtattcctg ctggagataa aaatgatcgg
    51121 tactttcgat acttcacgta ttttttgaca ccaataatag ccatccaaaa cgggaagatt
    51181 aatatctagt aatacaagct gtggttcttc tctttcaaag tctgagaaca cttgattgaa
    51241 atcggtcgtt ccaaaggttt caaattgcca tttctcaagt tcctccatga tcaattgtcg
    51301 aatgatctct tcgtcttcga ctaccatgat ttttgccata aaaaatccct ccatctttct
    51361 tcttgctact acaatatcaa agaagccctt gtgaaacaag ttggcttacc tggaaaatgt
    51421 cattttagca aacgttaaaa aaaactggaa atatgcgatt taatcgcgtt tgtctcttcc
    51481 aagcagctta aatttattat gttattctta ataattggtc tattatctgt tatttttctt
    51541 tcgttgtttt tgtcttttaa attttcgata acaccaataa ccgaaaagcg ttagcactag
    51601 caatatacct agaaggagta taagtaatcg ataaagatgg tatttttttg tctgttctat
    51661 ctttgaagag acttcttctg taaagggaac tcgtcttcct gtcactagta atcggtgagt
    51721 gttcacggtg taaggtgtac aagtgataag tgtgactaga tcctgctttt ctctaattgc
    51781 taatgtattt gtttttgttg gaaggacaac aattttctca acgacttcat aagcgagcat
    51841 ccgaccatac acttcaagat agaatcggtc atcttgctcc atttcttcaa gatccgtaaa
    51901 gagttttttc tcagcaatac cgctatgggc ggtaatcacg gaatgtgtac tctctcctcc
    51961 agttggaaaa gaggtgcctt gcaggactgt cgctcctcta tctaacaaaa gatcattcgt
    52021 ttcatcaaat aaaggcaagc taacattgat tttcggaata tagatggctc cgatcatatg
    52081 ttgttcgtaa tattcttttc ctggattttg gacatttcta accgcttgct caaaaggatc
    52141 tttgacttgt cccattcctg ggattactga tgtatgagca agcgtctgat ttttcttttc
    52201 atgcactgct aattgttgct catgtttttc ttctttatcg cgttcaagct gcttttgata
    52261 attctcgatc cttctttgat ccaaataatt gctgagagca tctgctaaaa atggatatgt
    52321 aaaaactgca gctccggcaa gaaacatcac tgccataaat aattgtaata aaagactgat
    52381 ttgggtttga ggtctattac ctattttctt tttcattttc ttcaccttct tatttaaaaa
    52441 aactgcagca acctttataa aaccgattgc tgcagcttat ttttgttgct tatacttcag
    52501 acttcatttt agatttacgg taccagctgt acgctccaat catcaaactt attccgatta
    52561 gtaagaatgc caagagcccg ttcccaccag tagcaggaag tgacccgcgt ttcatattcg
    52621 gtactctttc tggccctgga ttttctactc ctacgatctg aatttcttca ttatagcttc
    52681 cataagagat gataaattca gtaaaggctg cctcacctgt tggaagtaca taaccttctg
    52741 gcgccatggt ctctttcatt tgataggttc cataatctaa cccttgaacc tcaaagtatc
    52801 catttccatc tgcgacaaat tctgttgctt gtccgcttgt tgcccactca attctatctg
    52861 ctcctgattc ataaacagct aaaggcgact cccctttata aaatattgcg tattcagaac
    52921 ggtcttcatt ccacaattcg aatcttgcat ctttaagcaa ttcactgctt gatgcatcat
    52981 atttttcaaa ctttcttcca cctgttgtga ctttctctgg cggctctact ccttcttcat
    53041 aatcaaattc atcatttcgt ccgatttcaa taaccgctgt gttgttatgg aaatcatccg
    53101 cttctagttc tgtggaaacc ttcaaattat acgtgatcgt taaatctcct ccacgtgcat
    53161 ctgctaatct acctaaagta tctactttgg ctactccatt ttcttctgtc aaaatcgtca
    53221 cgacaaaccc gttgcttact acttctacta tataatcttg tccttttgtt aaaatcgtct
    53281 cgccggctcg cacttctatt tcctgatcat aataagttag accaactgta ggcgtatctg
    53341 tgattttgta atttttgata acagcgcttc cgttttcaag gacagaatcg atgaaataag
    53401 gaataggtgc tgtgatcgtg taagaagccg tttttcctct ttctagatca atataagaaa
    53461 tcgtttcttg ttctccagct tcattaataa tcgttacttg tctaaggtcg ctttcctcta
    53521 cattcatctg tttatctgct tctctgattt cgtttttcgg atacaaatgc acatctgtat
    53581 tgtatgtgtc tttccatgta gaaccatccc acacgccatc caccatatca ggcatcatca
    53641 ctggcatcat aacaaccatt ggagacgcga ttctggaaat atgagctggc gaataagttt
    53701 ctaagaaaag atagacttta tctctagaag aaggtgcttc ttccgtcact tggacggtag
    53761 agaataccgc ttctccagct tcattcgttg tttgtttgtc aatacttgtc agtcgatatt
    53821 ttaaaaacca actttctgtg tgccgttttt gaatccattc aatcgcttcg gcagaattaa
    53881 gaccgtcttc tcgttccggt gttaaaggat tatctcgaat caaatcgtaa tatacttccg
    53941 ttacgtcaaa caaatcaaaa tcgactcctg gaatattttc ttcaccaaaa tccgtagaaa
    54001 ccaatccgct attcggtcgt tcttccggaa cagatgagaa accttttttg tgtaatgtaa
    54061 tcgtcacttc acttggtttt tgtgcgactt cttcggcttt gactagtttt ccgttcccga
    54121 aaatccctat tcccgcagta acgagtaaca gtacactcat tcccgtgaca atcatttttt
    54181 tattcatttt tttctccccg ttcttttttt attaataaaa cagcacctaa caataaaaga
    54241 attcctagta aagaaatcaa cgttcttgct tcattcgttt gcgggaatac ggaccatttt
    54301 cctgattgac ctggattgct tggaccactc ggctctttcg gtttgtccgt tgtttctttt
    54361 acggtctcgt tataaacacg tggtgttgcg gattcatagg cagagactgg aactttcccg
    54421 gattctaatt cagccatctc ctgtccgtta actactacat attgtggttg gccttttgca
    54481 tccacccaaa attcagggat gatgacctcg attctcttac ttgcttcatc catctcgtaa
    54541 ccttccacac cttttaattc ttcaaaataa taggtgccag aaggcaagaa acgttgtcct
    54601 gtcgtaacaa ggccctcttt atccgaaatg aatttgctta catttttgtc attcaaagga
    54661 tcattgtctg ctggtttgat ccattgattt ttcaagtcat tagcaggagc catatctaaa
    54721 taatatttcc ccccttctat catctgatat agagcaaaaa cagcaccttc taatggtttt
    54781 cctttttcag ttgttcctct ttgacgtcca tacttgaaga agtatggatc tcttagataa
    54841 ccgacatttt taggatagat atgaatctct tgcagttcct cggttgattc tgtcgggtga
    54901 taaattggca aaatagcagc aattgggata gctgtctgat cgatatcaac atccaatcca
    54961 acttctggat caatcgattc ttcgaaaatc aaataaactg catcccgatc ttggtttttc
    55021 ttaggtgctt tgatacgtgc aatcccgtct cctaatccgc caaccgtatc tttcccagtt
    55081 gttaccgaag tccctatttg gacaagcctt tcttctgtga ttagctgacg gatattgttt
    55141 ctgttcgttt cagcgatttg ctggacgaat tctttaggag tcatgttttc agcagctttt
    55201 ttttcatgat aataatcggt catgtcgtaa atggcaaatg tcgctccatt tagtggcacc
    55261 gtctgtgaaa tgatatctgc tgtttcccct gcttcctcag agatagccaa gccatcattt
    55321 tcatggtatt caaattgatc gttataatcg atatctcgaa tcatccgttt atgcaacacg
    55381 aattccacga catcttcccc tgattcttct gccgacatcg catgtggaag aaggactgct
    55441 gcaagcggta gtaaaacaag taattgaagc atcaaacaca acagcttttt cattaccttg
    55501 ctccctcctt tttagattgt ctgacaaaca aaccataggc tgctcctgca agcgccatga
    55561 agaatgcagc aagcaggata tagaataatc gtccggatcc tcctgtagca ggcaacggat
    55621 tttcaggatc gttcccgaca ctaagctgta atctgattcg atttgtctcc aaaagttcat
    55681 attctaactc ttgctgtaga tcaggcgtct ctgcttcttc gtttggataa tacgaaatct
    55741 caacttttag tgtacccgct tcttttcctg gaactacccg atcattttct atgtcaggtt
    55801 cttctcgata actaatttct aaaataaagt aacctggaag ttttctatag ctgtcaggtg
    55861 acttcacttc cttgatccga tagacccctt ccaacaagtc tgaagcattg aatagggaag
    55921 aatcgtttgg atctggtgcc aagcctccct cgatgatttc atactctcct gcactatttt
    55981 gtttctcaat tgaaaattct gcgccttcaa gacgtcggct attcatactg tcaattttca
    56041 aaagttccaa atcgattggc gctagttcat cttctaaggt tacttgtagt acaccatcga
    56101 tgatttcata gccttccggc aagggagcag tttcgtcttc gatgaccgta tcttcaatga
    56161 taaacttacc ttcttcattc agtgcaaact ggaacacttg atcagatagc cgatacccat
    56221 ttggtgcgac agattccgag acctcatatt caccaataga cagatgcaga ggttggtcgt
    56281 aatttaaatc gacatacttt tctactccta cggcacgaga atagaagtat ccgatcccag
    56341 gcaaacgttt cgattcttct ggttctaacg gattaccagt gtatgctcga tacagcggga
    56401 aggaagaatc ccccactttc ctgatatcga aacctgcacc atctaatgta tggccttcat
    56461 ggtctaattt tctaactcgg atctcccgat aatcaaacgt attgtaagca gtgaatgcta
    56521 cccataaacg atcctcttca tctgttgtat acgtgagata ttgccaattt tcttcacctg
    56581 tttgcttgag ttggatagtc cctatagcat tatcgtaaag tcgcaagatc catgtgtcat
    56641 tttcatcagg ttctttgaaa ccttctgggt tctggctctc aatgattttg tattctgcat
    56701 agttggtagt ttcttcacta tccttataaa caaatgaatc tggctctccg attggatgaa
    56761 taactttctc accatccacg attacatatt tcccgtcttc atctactgaa tagaacgtga
    56821 gtctcccttt ctcttcctct acttcgttca caccagaaag ccctttcgct actggatatc
    56881 cgatttccag tccgttacca tctgtttcat aaagttcaaa ctctgcgcca ttgattggca
    56941 tattactgcc ttcaattagt ttattgatat tcatgtggat tggcttgaat tcatttcgca
    57001 tttccagttt gacatacaac tcatcatact ctgtgccttc tatgttaggc tcttgtacaa
    57061 cagaaagctt gtgatattct ccttcataat aggaattcgg atctttacct tccggtccgc
    57121 cgactctcga accagatgta tattcatcta caagctcgta gtgacctaac atctcatctt
    57181 cgttggctgc atattttttg atttggaatt gatacgtctc atctactaac tgataacgtg
    57241 gtggtccagc gatctctctg atccaaaata tgtcgctgac tgctccagtt tcatctccta
    57301 aaatcggcat cttcgtttca aagatcgcta ttccgtccgt ggtagtttta cttgtttcga
    57361 ttggttcggc ttccccactc tctggatttt ggtcatataa tgcaaattcg actccagcca
    57421 acggctcgcc attttgaccc ttcttgctga cttggaaatt acggccttta tgttcgtaac
    57481 gaatggaaga agatgggaaa tacatccgtc cagtagtgat atcattctca taatcctcca
    57541 ggttctgaat ataggccata ttgttcactg tgcaaaaatt tccgtcttga gctcccgcat
    57601 caagttttac ttggtacttg atcctgacca tttccccttc acctaaagaa atcggagaaa
    57661 atttataagc ttggttttcc cgtccgacat acgttgtcgg agtcgccact acttctggtg
    57721 gggcttgtcc atgacgttct tcatcccata tttctggctc ttggtacttc atgccgtttt
    57781 ctcctggatc tagatggtaa aactgaatat ctgccgatct gccagctcca aatggtgaga
    57841 cattaaaacc agtttcatca taaagtgtgg cttgctggat cgttctcttg aaggtagaag
    57901 agatatcctc caaggctgta acaatgtcat tcttgttgtt cgcattgtaa tatcgcggtg
    57961 ttacgccatc ttcttctaaa tcagcaatat ttttcaatac atttcgtcca atagcatcta
    58021 gtcgttgtct cccgctaacg tttccatcga ttcctaaacc aatggagaag atacttggcg
    58081 ggttctcttg atacttgttt ttcagccaat gatgataagc aactgtgttg atcgcagatg
    58141 acttttcttg cgaagtagga ctgccattcg tattgttatt agaagaacca tcaccaaccc
    58201 agtgagtcgc tttattatct ccttctttta cctctccata acgatagtgt acatttactt
    58261 catcttctgg acggttaaag tcagatgcat aggtaactgg atgagtaaac cctgcattag
    58321 aagtattccc agaaaactcc tcaaaatttc tgaacaaaac gtcattatct tcagcaatct
    58381 tatcttccat cacagtccag ttgctccaag ctccattagt gctcgtacga taacttgact
    58441 gaatcggtgc ataactgaag gtcggtgtcc catctcccac aacgattagg attttttctg
    58501 gatttctatt ttctccgcca ttgtctttat acaatgtttc atagccattt ttaagcccta
    58561 gtgtaagcgg agttcctccg attggtgtac gatttaatgc tgtattctca cttcccgtca
    58621 aacgattgat atcatttgtc aatggaaaga tgtccgaact gatttgctca tggttattga
    58681 tagctgtact attatggtaa aaattcacca taccgattcg aatatccaga ctcgggtcag
    58741 agagcagatc tttactcatt tcgtttactg cctcaatcaa cgcagcattt ttagtttgac
    58801 tattggtccc agcggttaat tcactcatac tcgctgactt atctagaaca aaaacgatat
    58861 cgattggact gatttccttt ttctcgcccc cgatgacatc tagatagaca ttgtaaaggg
    58921 ttgttgcatc ttcaggatgt agatcagatg ctgtgctgct tggcagaaca ggttctaccc
    58981 attttttgat gtacgccccg ttataatcca agtaactgtt ttcaaaattc cgttcatttc
    59041 cccaaagctg aacggtttga gctggaacgt ttctatcttc ttcgcctgca gttgatccat
    59101 tattagtagc atagtcgtaa tttcgccagt tcgctccccc gtttgtataa tcactatcat
    59161 cgatcaagtt ctctccgtca tatggcgagc ctttgtctcc ttgatagcgt tcattgcttt
    59221 gtgtccaatt tcctaacact tttgtatagt cattcgttgt aaacttcatc tattctttca
    59281 tcaaccacca cttgaagagg tagttaccct tcgttcacat cggggatctt ttctgtttca
    59341 aaaatcaatg tgccggtttc tgcttctttt gaataaactt ttccgacata ccattcttga
    59401 ccatcgatcg tctcgacttt taggtcttct ccatcttcat cataggagga tagacctgtt
    59461 ccacgaacgt tttttacggt accaatcccg gcggctgccg catcgattgc taaacgtatt
    59521 ctaccttcat tggcactttc ctttttttca aaagccagat gccattcaag ggtttcttct
    59581 ttttcaatat acttcccttc aatttttgca tagtcctcat ctaagaatac ctcatcctcc
    59641 tttgtttctg ttgactcagc agctacatcc agggtcgtca tatgatagac tgactggaaa
    59701 tatggtccaa atagcaaact gacagttact aactgcaaaa gcttcaattt taaccctttc
    59761 acatccttat actcctttca aaaatatttg tcttctttca caacaatcaa caaaacaatg
    59821 cccatctaat tataatttaa tttaatttta attttaaatc aaatgatatc ctttttagtt
    59881 aaaaacacca attatacaac atttattatt aaaattaaat tataaaattt tataacgttc
    59941 tcatttaaca aaacttaagt tattaagaca aatgaataaa caactacgct ttcctcgaaa
    60001 caaaaaaata aagcacaaat gcgcatttac gcactcatgc tttattcgta caattaattc
    60061 agataacggc agcgcatagg atgccaataa cacttcctaa aatagctcct cctaaaatgt
    60121 cagtaggata atgtaaaagt aaaaaaatcc tcgaataagc gatcaaacaa gcaaataaaa
    60181 aggctaatat tcctgcaact ggacttaatt cgaacaacgc agctactgat aaaaaggcta
    60241 gcgctgtatg tcctgaagga aaagaagaag tcttcggttt acttgtgtaa aggacaagcc
    60301 gacgattttt ttcataaggt cttttccgtc ttgtcgtttt tttgataaaa agcaaaccaa
    60361 caaaaaaata gatcaaactg ccgataagaa tggctagacc atattctcta gtatcaggta
    60421 tcaagactaa aacagcagca aggagcaacc aaataaaacc accattagag atattactga
    60481 taaatttcaa aaaattgatt tttcgactat ccgttttccg ttgcttggcg aacttgttga
    60541 taatactatc atcgatttta gcaatcaaac gcaacatgtt attcctcctt tttaagaacc
    60601 ttttcttgcg tacgatggat aaaaagcagg acttgaaaat aaaacaagaa actgaaacct
    60661 aataaagcaa aatagattgg tcctgcaaac cagaatgcga caagaaggat caacgtgcct
    60721 gccaaataga aaagcgcgct tttgaaatca ttaaaaaaca agaaaaatgc agtaatccaa
    60781 gaagcttctt tttgttccag ttgaagcaag attttgtaac taaagagaag cgcgcttaat
    60841 agaagcaaaa acaacgtagt atacatcaat gtatagccaa ttcccccacc ccaatagcgg
    60901 aaaatctgat aatcgatcaa tagaagaaga aaataaacac ttgctaaact atcttgcttt
    60961 ccataaagag agaagttctc acgaaacttt ttccagaata gtgaaccgag ctttttctct
    61021 tcattttttt tgtaatgata aatcatatac agaactgttc gacttgcagt tcctaatgtg
    61081 aaaacaggca agacgcacag gaaccatagt agctgtaaat ggataagacg tggaatccat
    61141 tcaatgattt ctaagaaata atcattggct tttttaccaa tcgatcgatt catcatcctt
    61201 ttgaacctcc tgtcaaatcc atcccttcaa tgaaataacg ttgtgcaaaa aagaacagca
    61261 aaatcgtcgg gatcaagaca agtgttgctc ctgccatcag ataattccat tgtgtcgttg
    61321 cttggttctg aaaaacttgc aagccaatct gtaacgtgta tttttcttga tcttggatat
    61381 ataacagagg acctaagaaa tcattccaag ctgcgttgaa ggaattgatc gcaatggtaa
    61441 tcaaggcagg tttcgtcaat ggaagcatca aatgactcca aatatacaaa tgattcgctc
    61501 cgtcgatttc agcagcttcg atcaattcat tattgatcga taagtaaaat tgacgcatca
    61561 gaaatatatt gaacgcattg ccgaagaacg atggaacgat caggggcaaa taagtaccta
    61621 cccagccgat cttggagaat aatacatatt gagggatcat cgttacaaac cccgggatca
    61681 tcatcgtaga aagaaccaaa gcaaacatca gctttttccc aggaaaatca agcttcgcaa
    61741 atccataagc aataaaagaa tttgaaagga cattaccgat cactactagc acagtgatga
    61801 ataatgtatt tcttgcatag cgccaaaaag gaaaggcggc aatcgtatcc agatagtttt
    61861 cccatacgat tttttctggg aaaaaggtcg gaggataacg aacgatttct tccattggtt
    61921 tcaatgaagt aaacaccatc caaagcaatg gaatgatcaa aatgattcct attactgtca
    61981 tgaggacata acttagtatt ttttttgttt gccgattcac actggtcttt ttgaaataat
    62041 ccatcatttg ccgtcacctc cagaataata gacccactta ggtgcaagct tcaaattgac
    62101 aaacgtcaga accaacacga tcagaaacag gatcattgac atagccatcg cataacccat
    62161 atcaaaagct acgaacgcct tgttccacat atgcagatta taaaaaagca aagaatttgc
    62221 aggtccgcct gttccatttt tcgtcatgac ataagcttct tgaaaaattt ggaaagaacc
    62281 gatcgtagag gtgatcacat cgtaaaaaat aataggagtg attaatggca aagtaatact
    62341 tttgaacttt ccccaaacgc ctgctccatc gatttctgct gattcatata aagatctagg
    62401 aacattttgt aatgtagcta agtaaagtag cattgctcct ccagaactcc atattttcat
    62461 caaaatcaac gcaggtttcg tccattgtgg atcaaacagc caagctggtc cagggatatg
    62521 gaaccagccc agaaatgtgt tgatcaatcc tgcagatgga gaaagcagct gcatccataa
    62581 gatgtagact gctactcctg ataagactgc cggtaaatag aaagctgtac ggaatacact
    62641 gattcctttg attttttgat tgagtactgt ggctaaaaac acactgaaaa tcgtcgtcag
    62701 cggtacattg aagatgacga aataaacggt gttgtataac gatttccaaa ataaatcatc
    62761 gtgaacgaac attcttttga aatttgctag accaatgaaa ttcatcttgg atgtcaaatt
    62821 ataatcggtg aaactaccaa taactgaaaa aagcataggc cctagcataa ataccaagaa
    62881 cccgataata aaaggcgaga taaataaata ggcccatctt gcttcttttt tctttcggat
    62941 agataaacgc ttcaaatttt cttgcctcct ctgtacctag acagtcaaaa atggattatt
    63001 gattttgctt gactaagttt tctactgctt tttgtgcatc tgccaatcct tcttttggtg
    63061 attttgttcc taaaagaatc tggtcaatct gttcattcac taatgtcgtg taatccggag
    63121 catctaaagg aactggtgtg atcaccgtat ttttaaagtt ttcgtccgcc atttgataga
    63181 tcatttttcc tttatccgtt aggttgtcat tattcactaa attctcattg gctgtcttgc
    63241 tcgccatgat atcaaaactt ttttcgccga atttttcttg tacttctgga gaagaaagat
    63301 atttgatgaa ttcataagag gcctcaggat cttttgctcc gtatggtact tccaagacga
    63361 atccgccacc ccacgaccaa tggcctgaac cttcttcttt ttctgggagc tgcatcacgc
    63421 caaaatcaaa atcatccggt gcattttcca tcaaactaga gtaataattg atattttggg
    63481 cacgcatggc tactaatccg gaaataaatg gatcagatac accagaccca aattcggctt
    63541 ctaatttatt gatcgtattt ttcccataat aatcttgcca gtcttggatc cactccaagg
    63601 cttccacttt atttttagta tcaatcttga ctttttcatc tttgtcaaac caactagtcc
    63661 cgttatctgc gtttagcgcc catacatcag cccctaaatt ccataatgga taaaaaccga
    63721 tgcgtgtaaa ttcttttcct tcttttttat ctagtttacg tgctgtttct tctaattcat
    63781 cccaagttgt cggtagatct tgttcactga ttccagcttc tttaaataac tttttattat
    63841 aaaaaatgac ttgtgtatct gtattgaatg ggatcgcata aggatcacct tcatattcta
    63901 ctgtatccca aagctgcgga taaaaatcct tagaagtgtc tttttctaca tatttggaga
    63961 gattagttgc ttgctgtgct tctgctcttt gggcaacgga atttatgtcc tgtacaataa
    64021 catcgggtgc atttccagct gttattgcag ataaagattt agtccagatg tcaccccatg
    64081 gctgatactt ataatttaca tggatctcgt tttgtgattg attgaaatcc tcaatgattt
    64141 cttcgatagc ttcttttcgt gcaccggatc cccaaaagga ccagaaatca atattttttc
    64201 ttccatcact tgttttttca gaagaatttg ctgcacttga actggcatcg cttgcattac
    64261 ttgatccgca tcctcctaat aaaaacaatg ctgaacttgc cagcaaccct gtaaacaata
    64321 attttttcat attttacctc caaattttaa tgtaaccgat gccggtttta atataacgat
    64381 atcattttgt tctgaaaata acaatttata aggtatgatt ctaaaaaaat tattaatgat
    64441 accaatactt tatataattc gcgagcgctt tctttgaatt ttttaatctt tcgtagcaga
    64501 atgagaatgt attaaaaata gattaggagg aattttgatg tttagacacc agaaagaatt
    64561 gcagtttgaa gcaaaaccag atcgtccgaa tccattaatc gcgaaatatc tgcaggagct
    64621 gatcggcggc caatatggcg agatgagtgt agctatgcag tacctgttcc aaggctggag
    64681 ctgccgagga gaagaaaaat ataaagatat gctgatggac attgcaacag aagaaatcgg
    64741 ccacgtggaa atgttggcga caatggttgc tcgtttatta gagggtgcgc ctgttgaaga
    64801 tcaagaagca gcgatgcaaa aagatccaac aatcggtgcc attatctctg gaatgaatcc
    64861 acagcacgcg atcgtagctg gcttaggacc tcgtcctgct gacagcgtgg gcaatccatg
    64921 gagcggtgcc tatatcattg cgagtggcaa cttgttagca gactttagag ctaatttaaa
    64981 tgccgaatca caaggaagac ttcaagtagc acgtatttac gaaatgatcg atgatcgtgg
    65041 cgtaaaagat atgctgtctg ttctccttgc aagagatagc tatcatcaaa atctatggga
    65101 atgtgcagtg aaagaattag aagaaaaaga aggttcaatc ttagtcccaa gtactttccc
    65161 gcgtgacaaa gaacgaaaag acattgctta tgacttctat aacttctcag agggagacca
    65221 aagtagtgaa ggttcttggg ctagtggcaa agcactggat ggcgaaggcg aatatcaatg
    65281 gttgaaagaa cctaagatcg aaggcgctgc accaaaatta gaaccagtaa caccaaaaat
    65341 gtatgggaca cctccattca agaaataaat atctacccgt agaaacaccc gttttttgtt
    65401 ccacacgctt tgtgagcaaa actgactaaa gtcattaatc ataaccaccc ttcaaaaggg
    65461 tggttttctc tagagctata agctcaaatc accggccagc gcctaaagac gctggctttc
    65521 atattttatg ttcaagccat attgcctttg ccagttttgc ccacctctta aaaggtttat
    65581 gtactactcc ttagccctta aaaggatcgt cttactctcg tacactcagc ttatctaacg
    65641 cactatcatg tttttcttgt tcttgaatgt actttttgat ggtcgcttca ttttgtccta
    65701 cttttgtcac atagtatcct tccacccaaa agtgtcgata cccaaattta tattttagat
    65761 ttgcacaatc tcttgatgat ctcctgaata ttggctcgat attgattata aattattttt
    65821 cttctatact ttggtgtgaa tacaatgtgg tatttacaca tctactttgt acgggctaaa
    65881 ctatttccct ttattgctcc acccgcatag caagtggttt tgtatttcaa tcgctcggcg
    65941 cgctcaatta ggtctaaaga caataaatct aaaaacccgc tgcttcactg acagaaacaa
    66001 cgggttttca gaaaaaaatg aaatgaaaaa gatctcttac ggcaatctat tttatttttt
    66061 tgatttttta taaatggaaa atgcttcaag aagtccaaga aataaagccg tataaaaaca
    66121 agaacctatg aaatctttat ttattagata ttcaacagaa atcccgataa cactagccaa
    66181 caatatattt atcagaagtg taaaataagg atgcttttta tagaatagaa ctagtgttgt
    66241 tttcatagac tctcctttct tcatatttcc aattaatcac aaattcacgg cattactaga
    66301 aaaattaaag gggcgcagaa gtggaaaaat actcttcgac tttccctaca aaccaggcca
    66361 tcttgtgttt aataactata ccaaatctaa ggtacctcta aatctaaatt ccatttgtgg
    66421 cggtgacctc caatttttaa ctcttctatc tcccatccag tatacataac cttgatactt
    66481 cgaccactta tatatcgaac acacaaatac ctatccattg caggagtact acctaactta
    66541 gttgctactt gcatctaaat aacagctgaa aagataggac ttactcttag taaagtatct
    66601 gaactattaa tttttaaact atcattttta tcctcaacta catgcacatc tgttgcaact
    66661 tcactttctg tctcatccag ataaatgatt aaggaatcgg gaattgcaat atcttcagct
    66721 gacgctggaa tagttgagct aaattagtca tcatatcttt attataaaat aaccaacagc
    66781 aaaaaataca atgaaaaaca aacttggaac taatgtatca ttaataaatg ttgaaaaatt
    66841 cagcaactca ttactgtatg attttagcag cccaaccaac atatacccta taaaagctaa
    66901 taagatggtt aggaatcctt tagaattttt actcattcat agacacttcc ttaaataact
    66961 tttattatag aaggtattgt aaataataat attgacaaaa agtgactcaa aataaaaaaa
    67021 aacttacatt atactggaat gaactgtatt gatagtgtac ctaaaagctc tactgacata
    67081 agatcgtgta atcggtaaag taattttatt aattatacgg gatgtaatca attagagaat
    67141 aaactaatca taaaaggtac ttttacattc ataattgtcg ctttgagatt ctactttggt
    67201 tcactatctg acccaattgc acattttcga tcattacaag tattggtgtc gttagaacaa
    67261 ctaaataaaa actagcggta gatttttcag aatctatgct aatttctgta tggatgttcc
    67321 agtgatggca aatttctctt ttcattgttt ttcttgtcgc atgatatgcc tttttgtgtt
    67381 attcagttaa actatagaaa ttctttccta gacagatctt gatcccaaat tttacgtttt
    67441 tgagcatcga tatagtttaa aatgacatta ctcatatcta aagagtttag attttttagg
    67501 actaaaactg tcagttgttc tttttcttct ttctgttaaa gtattgtcgc gacttttcgt
    67561 atacgttaag atatgtcgtg aaaaaacaag ttttgtgaca caaaaaaacg aatgacttga
    67621 ggaaatcacc tcaatcattc gtttaaaatg ctatatattc tttagtttaa gatacggaaa
    67681 ttaatgccat cgaccacact agtagaatga acgacattta cttggccttt ttttaatttc
    67741 gttatgttta gcatataaaa atattataga gtttacttac aatgcttaaa acatatactg
    67801 tataatctga tcaattttag ccatgtcttg caatggtaag ttttctattt ttttgagttt
    67861 tcgttcctta aaatcaagag attttaattg tgtaataatt acttgtcctt ttatatctag
    67921 ctcttcaggt aatgagtaac gtgtcggtaa attctttata gtggaagtaa ttggacatat
    67981 gaccgaaaac attgttttac ggttaaattc ataacgagag acgactagtc ctggtcgtct
    68041 tttttgaatt tcttttccta cggagggatc aaaatcaatc catacaatat cacctttttt
    68101 aggaatataa attctttctt ctgacactat aaaatttctc tcccttcagg agttaaatct
    68161 tcccattcgt ctttctcata atactcggcc tcttccccac cactaaatgg atcttcaatt
    68221 tttggaacta acgtaattgt tccatcatca gaatacacaa cgatatattc ttggttttct
    68281 gaaggtttct ttccgttatc agaaggtaaa gttaccacta cagaacttcc ttgtaatcta
    68341 gattttgtaa caagcattat catcactccc ttaataaaag tatactatat aacactaagt
    68401 aacgcaaaat tcccctttgt aaactatcta gaaaattgtt ttgtttaaag tatatcttat
    68461 tggatataaa tagaaataaa ttgtagccag ttcatttttt ctttaactaa aaaaataatc
    68521 caagaaacat tgaacctatg gatccaaaag ctacccaaat actatagcta accccccgct
    68581 accttcaatt ttgcagataa aacgctttgg gagaaaattt caaagacaac aggccatcct
    68641 atcatgcccg attataaaca aaaagctggc tattcctttt gctctgacaa atttcaattg
    68701 ggaaagttca cgaaactagc aggtgaagaa gtttcaactg ttcggatcaa agaatgcccg
    68761 cttcaaatag aaacattggt tcaacgaatt tcaactagac aagactttgc tattgtagaa
    68821 cgtgagatcg tagctatttt tgtagaagaa gggattttac atgatccaac tcatatcaat
    68881 gtggataaat ggcaacctct tatctataaa ttcagagaat atacaactgc caaccagtcg
    68941 ttaactttga attttagatt ccaagaattc aaagagtcat aatgataaac atcgttaggt
    69001 ataaataaaa aagactcgca gcacgagtaa aaaacaaata gaagctatac attagaaagg
    69061 tcgactgtga ccctatagat gcagtatatg cagcttatga gcatttcttc tgttctcttt
    69121 gtttttggga tctgtgctga agtctttttc gttttttgcc gctctttctt tgaaaaatcg
    69181 attatgttat accttctcca ttactaattc aggcatctgc tgagtaatga tttttcgcgc
    69241 aaaagaaata tctgttaccc aaggttcgat ttgttcttca gtcaagacaa gcggcattcg
    69301 gtcatggatt ggtgcgactg tgcggttagg ttcagtagtc attatgattg attctgccat
    69361 tttttcatcc tgatggctac gataaaaacc gccaatatac actatttttt ccttatttga
    69421 aaacataaat ttgtcttttt cttcattcca ctcattggga aaacacaacg attttgcaaa
    69481 aatggttgat gaaaggtctt tttttcttct actgtctctg cacgggcatt aatcatcaat
    69541 tgtccttttt taaatccggt aaatccccac ttcatgattt ccggagtgag gatgttttct
    69601 tcctcttttc ttaacgtcac aacaagattt gacggaaaaa cttctccagc tgctaattct
    69661 gctgcctgct tcggttgttt cgtctgggct tctcgataat aattctgaag gagctctgac
    69721 tctagatcaa aaaaataacg accacacatc ctaatccctc ccaatctttt tttctatcat
    69781 acactgcaac aagaagttcg caccaacaaa acgctttcaa tttttgtaga actaaaaaag
    69841 ttgtgacaag tgacaaactc tccagtgaat ggtattctcc tgccaactcc tcggtcacaa
    69901 gtcacaatat tcaacaaaca tgagaagctg ctttttgaaa ttgttccttg tgactcggag
    69961 tgaaacggca ggcttacgac ctctgggtaa acggtgttca aaagccagtt tctcggtatt
    70021 aagccaaatt tatgcaaaaa tgtagaaagc tattttcaca aaatttttct taatactcga
    70081 aactaagcga ctttttcaca acctcttcct taaacggtgt tcaaaagctt gcatctgcgg
    70141 taataagccc aaccaaacga aaaatatgag cagctatttt tatttggctg ttcttattac
    70201 ttgatgcagg gcagcttttt cacaacctct ccactaaacg atgttcaatc agctaacctt
    70261 cggtattagc tcaaaaaacg gcaaaatatg aggaactatt ttgccgtttg tttctctaat
    70321 actcaggtca aagcgctgat ttcacaacct ctaatacacg gtgttccaga agtagcttct
    70381 tcgaaaaata agccgaaatt tcacaaaaat ttgagaagca attttcgtaa attccttctt
    70441 atttcttgaa gctgaccact tctgtcacaa cctctaatta ttaaagaacc ttttgcgaag
    70501 ttaacaatct ttctttgatc aattgaaaat tctgcctaag tagtgataga tcctgtgtga
    70561 ctttcaacaa taagatactt ccctggattt cagcaatcaa cgctttggct tgacggtctg
    70621 cttcttcatc ggacagtttt tcttcagaaa gtgccaggat attttgtttc gtatacttga
    70681 cccattgatt gactaattct agtagcaaca gacggtattc ttcatccact gtactcaact
    70741 caataatcaa attacctaca ggacagccat gataagtgat ctgtgtctca tgatcagcga
    70801 aaatccagtc caacatttct tctaatgctt cttctggctg ttttccagca tttaagatgt
    70861 cttcgaacaa ttctgtctgc catacgtcca tatgcgcatg gatcaccgct agacaaactt
    70921 ctttttttga agaaaagtaa taataaaatt gaccttttcc tgtatcagaa gctgctagaa
    70981 tatctcggat tgacgtttca tgatagcctt ttttataaat aagatctttg gtcgcttcca
    71041 agatcttttc ctttgttttc aatgatattc tcctttttca tttctcatag tcctatatta
    71101 tacagtaaat tcatcatttt ttcttcttaa tcgtttaaaa aagactgtgc caaattatct
    71161 gacacagccc tcactataaa actgttctca tatagctaga tgttcattga ttcaagaact
    71221 gcgaggacaa aagcaattcc tatcttatga tagttgctct aatacttcct ctgttgtcat
    71281 cacttgatct gcaataaagc caaagttcgt caacgctgca tccattgctt cttggccagg
    71341 agcaccaatt gcatctgtcg ctaccacaac attgaatcca ctctctacca attcacgcat
    71401 atgggaatcg ctacacaagt ttccgttcat tccaccaaga attactgtgt cgattccatt
    71461 ttttctcaat tggagcgcca aatcattgct ttctggtcca aagattttat gaggagagct
    71521 gatgatcgta ttttcatcca aataaggttt caattcttcg ataaaatcgg ctcctgaacc
    71581 ttcttccact gattcatatt gactttttct agcaaacatc tgctgctcaa gcatcatatg
    71641 ttctcctgca ccgccaaatt gccatttatg gtcatgtggg taaaaataat gaggagaaat
    71701 aaagagtttg tatccttttt cttttgcttg ttttaccatt tcaatcatat tttcgatcgt
    71761 attgtatttt tctaagatat cttttgtcaa accaaagcca ccgcctgctg gtttcaagaa
    71821 ctcgacttgc gggtcagtga gaactactgc tgtattttta ttattgaaca tggtggattc
    71881 tccattcgtt atttatttta atacatcatt tagagcttca ctcattgtcg gatgagaata
    71941 gatttgatct ctcaataacg tataaggaag ttttccattc atagctagcg aaatcaagtt
    72001 gatgacttca tgagaatctt ccgcatagat acttgcccct agaatcaagt cagtttcagg
    72061 gtcgactaaa actttgaata cacctttagg atcttctaat acttgtgctt tagggacacc
    72121 gcttgtcata tacttgaata atttgtaatt gacgccttgt gctttggctt gtttttctgt
    72181 taaacctaca ttggataaag tcggagtgat aaatacactg taaggcacag ctttccggtt
    72241 attcgttgtt cgtttattct cgcccttcaa ctgatccata atgattcgat aatcatctag
    72301 agagatataa gtgaactgca atccgccttt gacgtctcca atcgcccaaa tgttgtctgc
    72361 atctgtacga aggtattcat ccacagcgat tgctccgcga tctgttagtt taacatctgt
    72421 attttctaat ccaagtgatg ctgtacttgg ttttcttcca gttgcagcca aaacttttga
    72481 cgcttggatt gttacttctt ggccatcttc tgtaaaggtg acggcagctg ttgattcttg
    72541 gtctgctact tgatctacag atacacctag atggaattcg attcccgcat ttgtcatgtc
    72601 ttctaaaatc atttgcgcga tatcttcatc ttcacgtgga agaaattcag aatgggcatc
    72661 aagaacgaca actttagatc cgtattcatt gaacattgat gcaaattcca agccaatata
    72721 tccagccccg atgatcacta atttttccgg taattgttcc tgatccatcg cttgcgtaga
    72781 atcaatcaag tagcgacttg tattcaatcc tttgattggt aaaatgattg gttcagaacc
    72841 agtattgatg aaaatccgtt cgccttcaac aatcactttt tcaccattat tcgtcacttc
    72901 gatttgatgg ttagaaagaa aacgtgctgt accgtccatc actgtcccag ttgcctcatc
    72961 gctgatcata tgataatttt tggcgcgaag catgcccgtc aatttttctt tccgagcaac
    73021 agcctctgta aagtcgattc ctctttctcc attgaaaatc aatgatttag aaggaataca
    73081 gccgatgttg atacatgttc cgccatacat acgtgttgac ttttcaacga ctaaaaccga
    73141 ttcacctttt gaagctaacg ccttcgctaa agtcttaccg ccctttccaa aaccgatcac
    73201 aatattttga tatttttcca tcatttttcc ctcgcttctt ttttacctac cgattggtac
    73261 aaaaatatga taaccgataa ttgtctattc tccaaatcat ctgctttatt tgtcaaaaaa
    73321 aaccagacaa aaagcggtcc tttcacgttt ttagtctggt tcgatttcct ttattcttct
    73381 aaacaatcgt tacttctaca tcgatatttc ctctagttgc tttggagtaa gggcagaatt
    73441 catgcgcatc ttctgcaatt ttttgggctt cttcttcgct gatgccatcg atatgcactt
    73501 ctaatactac accgatctga tagccgccat catctaaatt ataaagagag acacgtgctt
    73561 ttactgtgga ctctgctgtc attcctgctt ctttgatcat atgttgtacc gccccattga
    73621 aacaagaagc ataacctgct gcaaataatt gttctggatt tgttccttct gcatggattc
    73681 ctggaggagt gattttcata tgcatcgagc catttggtgc ctcgacttct ccattgcgcc
    73741 cgccatggtt gatcattgct gtttcgtatg cttttttcat tgtactgctc ctttcgattg
    73801 accatttgct acttggctat aattatagtg tatgacaagt aaggcgcttt ctacaaaaat
    73861 tctgcttatt cgaataccag aatgccgtct tctactattt ttccaaaaaa caaaaaaata
    73921 ctactcgtga acaacagcgt catatttgtt ccaaaataaa tagcaatctt ttctacataa
    73981 tcttttatat gattgatttt aagatacata tataccagaa agttgacccc ataaaccaat
    74041 actgttaaaa aaataaccat attcgttacc attttttcct ccttttgctg gtctccctac
    74101 cagcactcga tttaaccata ccactttttt tgccgtccaa gcaactagcc tttcaaaaag
    74161 actggtttat tctgctgtta atgattcaag atcagaaaaa tgtccgaata attttgctaa
    74221 ttttactaac gccaacttgt tctttccgaa ttcatcattt tctatctgta atactagtaa
    74281 cggctcatgt aagcttaaac ggatcaaaaa ccagccgttt ccgtatggct cttctagatc
    74341 atatcgaata ccttcttcat tttccgggtc gaagttcatc cctttttctt cttccaacag
    74401 tcgcttcatt tcttctaata tcttgtttcc ttgttcgcgt ggttcatcgc tgaacaattt
    74461 taatcggatt tcttgtactt ctacgggctg tttcaactct ttgatttgtt catctaatgt
    74521 gatacctttt ctttttaatt ctggcaaaag catcaggatc tttgcgatga catatgcacc
    74581 atcatccaga aaatagtttt ccttgaatgc agcgtgtccg cttgtttcga tggctagctg
    74641 tgtatctttc ccaagacggt taagttctaa tgctttattg attacatttc gatagccgga
    74701 aatataacga acttggatgc ctcctttttc ttcaatgaat ctttgtagat gagaagaagt
    74761 aggggaattc gtcacgattc ttgttcctgg atgctctttt aatacgattg tacttaatac
    74821 tgcgatcaaa ttatttcgat tgatcactgt tccagaagca ctgacaactg cagaacgatc
    74881 cacatctgta tcgaaaatga tcccaagatc tgcttcattt gctaaaacag cctgcttgat
    74941 actttccatc gcttctttat tatcaggatt tgggatatga ttaggaaatc ggccatctgg
    75001 ttccaaaaat tgggaacctg ttgaatccgc acctaagcta ttcaatactt tttcagcaaa
    75061 gaatccacca gcaccatttc cggcatcaac aatgattttc caaccattta gtggtttgct
    75121 ttctagtgtt ccaatcccct ttttaatttt tgtgactaaa tcttgcgcat aaggtgtcaa
    75181 aaggtctgct ttttctacgc ttccagtttg ttcgcttttt attttttgct gctgtgatag
    75241 gataaaagca atatcttctt tttctgctcc tccttctgag gtaaagaatt ttattccatt
    75301 ataataatag ggaagatggc tagcagtcag cataaccgca gcatcgcacg agaactgttc
    75361 aaattgagtg gacataaaca tagcaggcgt agtagctaat tcgaaatcga tgacatgtat
    75421 cccttgttct ttcagacttt ggatcaacgc ctctttcagt gctggtccgc ttatcctact
    75481 gtcatgacca atcccaatcg tccatttttt atttggatcg gttgatttct tctttttctt
    75541 tatccagttt gctagtcccc agccgatctt ttttaccatg tctgtcgtca agtttacttc
    75601 atatccttca actggtaatg cgattcctcg aatatccgat ccattttgca gcgctttcat
    75661 atctttcata taaaagtcct cctcatttat tccctcattt tttccagctt ttctgcaaat
    75721 cgctgtgctt cttcttctga gcggcaaata ataatgaatg tatctacacc ggccatcgtt
    75781 ccgacaactt cagggtaagc cacttcatct agccaattcg ttaccacatc ggcaccattt
    75841 cgttcagtca atacgataac catgaactga acagactgga tacgcaaaac ctcgcgtcta
    75901 attgctgtac gcaaacgttc ttcatttaaa gaaacagatg gctggttaaa taatgtatag
    75961 cgtactattt tgttttcatt ttgacttttt actaacttca tttctcgaat gtctcgagaa
    76021 atcgttgctt gagtcacatc ggcgcctgct tcatgcagcc gcgcaagcag ctcttcctgt
    76081 gtcccaatag gttgttctgt aatgatctgg cggatcagtg cttgtcgctg tttctttttc
    76141 atattagaca cctctcaatg ttgattatag caaaagataa gtaatatttc ttcatatttt
    76201 ggtatttttt acttttattt tttgttttca ttcaggttgc taaccttttt ctattttaac
    76261 acgcaaaatc gtcttcattt tttcaggtgc tgattttctt ggatcgctga tgtaaatctc
    76321 tttgtgttct ttagaagctc tttcatagcc ttcttttgcg atgaatgctt ctaatttctc
    76381 aaacgttgct ggttcttcgg catagggccc gacgtgtaag atttgggcac aaagaccttc
    76441 ttccatcttt ttgaatacta cttgttcttt caagtcttcc ggaagtttgc tagaacgctg
    76501 aatggcttct tgtgctatcg cttctgtaac aaagtcaggc tgtcggatca tcaattgata
    76561 agaaaaatgt tcttttttca tcactggttc atgcaaatac ttttcttgca gtccccattg
    76621 tccttccagt ggatagacag tatacatctg ataatcagga atcagccaat tttttttagg
    76681 cgacatgcgt attgcatagc tgactgcata tagacaggct actcttcgtt taaagtcttc
    76741 ctgattcggg tcgccgatcc ctctgatgct aaagaatgat tttttaggaa cctctagtat
    76801 ttgcgggctt tttttcatac tatagctctt gtctttacgc cattcaattt tttccatttt
    76861 tcttcctccg cgaaattcca tttagttatt tattatcata cctctttttg agtgacttgg
    76921 ctaatcgtgt tgacactatt tttcagtttg tgctagaata agtgaagtaa ataaatcgta
    76981 cagataacac gtttcggcgt gtcgagagag aggcggtttc tatttttata atagaaatca
    77041 ctctcttttt ttacgaaatt ttttagaggt gatgtaaatg acaaaacaaa aacagctgct
    77101 tttagtacct gcagtaattg caacagggat gatgtctttt gcgggcgtat tgatcgagac
    77161 ggccatgaat gtaactttcc caactcttat cagacagttt gatttaacga ctgcacaagt
    77221 ccaatgggta acaacgattt atcttttgat gatttcgatc attgttccgc tgtctactta
    77281 tttgaatcgc aattttagtc tcaaaaaact ctttttagct tcaaatctgc ttttcttggc
    77341 aggtataatg atcgatttct tctctcctac cttcatggtc ctattgtttg gtcgattgct
    77401 ccaaggtgca gcgacaggta tcgctcttcc gttgatgttc catattatct tgacatttgc
    77461 ccccctcaat cgacgaggcg cgatgatggg tgtcggtact ttgacaacgg ccattgctcc
    77521 agcaattggc cccacttatg gaggcattat gacttcacat tttacatgga atcatatctt
    77581 cctgtttctt gtgcccgttt tgtttctttc tttgattatc ggctggtttg ctattcccga
    77641 gatggcagta caaaaaagtg gttatttgga ttggataagc gttttaggaa tttgtctttt
    77701 gtttagtgga agtctaacat ttttgagtac gttgggaaca gtttccagct ggattgcttt
    77761 aatcatcggc ggtattggct ggttcctttt ttactatcga agtaaaaaag tggagcatcc
    77821 actgatccga ctgtctattc tttcaaataa aacattccgc ttgtttttga ttggcttctt
    77881 agtttatcag tttttattac ttggtgtatc ttttgtcttg ccgaatttcc ttcagatcgt
    77941 tcaaggaagt agtccttttg tcgctggatt agcgatgctt cccggcgcag cggttggtgc
    78001 tttgctttct cctttttccg ggaaaatgct ggatcaatat ggtccgaaga agccaatttt
    78061 tgctgggctg attctttctt tgattggttg gctagcatta ttcctactca taggacacgc
    78121 ttcagtgggt gtattgattg cttgtcatgt gttttatatg atcggtatcg gattatctta
    78181 cagcaatatg atgaccaccg gaatgaatgc actgaatgaa gaactacaag gagacgggaa
    78241 cgcaatgttc aataccttgc agcagttttc tggcgctgtg gctacttcat tagtagcggt
    78301 cattatcaat tacgtccagc atcatacttc ccataactat gaagtgtcta ctacattagg
    78361 atcaaaagca gcattaggcg tcttgttgct gttgcttctc gtaagctttg ctcgattcgc
    78421 ctattatctg ttctttgcta aaaaagcgta gattccatat acggcaggtg ttgcatctgc
    78481 cgtttttttg tgtgaaaatg gttgtttgta aaaggaggct tttttatgga aaaaattgcc
    78541 attatcggtg gcgggatcat tggtatgacc ctagccagtt acttagatac atcaaaattc
    78601 aatgttgctt tatttgatga agggacagga caggcaacta aagctagcgc tgggattatc
    78661 tccccttggt tgtcgaaacg cagaaacaaa caatggtatc gtctggctaa agacggcgct
    78721 gcttttttcc ctaaacttat aaaagatttc caacttgatc aaacgattta cgcacaatca
    78781 gggacacttt tgcttcgacc tgaaaaagat ttagctgaat tggctgttct tgccgagaaa
    78841 cgaaaagaag aagcgccaga aattggaaac atcgaatggc tttcacctga acagacatct
    78901 caagcacttc cgttattgaa accagcagtt tcactgcgta tcagtggtgg tggtcgtctg
    78961 gatggaaaag catatttaga acatcttggt cagcgtttag cacaaaaaca aatggagcag
    79021 atccaggcaa aagtcacttt gcataagaag agcgattctt ggctcatcaa gggaccgttt
    79081 ggagaataca cagctgatcg tgtaattttg acacctggcc cggcacttaa gcctttatta
    79141 caagaattag gctatgctgc agatatacgt ccgcaaaaag ggcagctctt agcatttcag
    79201 acatcttata agatgagtca agactggcct gttgctatgc ttgacggaga agctgatttc
    79261 attcctttca tggatggaaa gatcctttta ggtgctaccc atgaaaacga tcaaggctgg
    79321 gatttggctc ctactagcga agcttataag ggattgacta agagtacaga acagttcttg
    79381 actgatccag atcagataaa aaactggcca tacgagttcc gtgtaggtac ccgtgcttat
    79441 acgtcagatt tttccccatt tttcggcgta ctgccagatg atccaagctt actggctgct
    79501 agcggtctag gctcttctgg cttgaccaca ggtccttata tcggctatct attagccaac
    79561 ttcttaaata ctggtcattg gcctacggaa gattatcaaa aggacatctc cacctatatt
    79621 acgaaaaatc catcatagaa aaacagcttt ttcaagtaag gatcccgagc gatattgcgc
    79681 atggattact tcttgaaaaa gctgttattt tagttcaatt tctgaatcaa attctccaaa
    79741 ctttcttttg cttcatctgg caagagatct tttcctatct gagcttcaat gaatcgtaaa
    79801 cgaatatcaa gtcgttgttt caccagattt ttataggacg tatcctcaaa agaaacagga
    79861 aaatctggta taggtaaata gtggatgtct tcaaatggac caaacgactt gaattctgcc
    79921 gtttgttgca cgattctttc aacgattgac aatgtcgttt caggtttgat ttttttatca
    79981 tcgaaatatc cagtattcag gacatagcag gctgtttgct gtttcaaaaa caactctcga
    80041 aaagcttgat agtcttccct aagaggatag acacggaaag gattagcaaa tggttcaatg
    80101 actagctcat ctagctgtct ttttgtagtg atattttccg cgtctgatcg ttttgttgct
    80161 aatgttgcac caaaagacgc tgctaaaact gggtcattga tcttgataat tggcggaagc
    80221 gaatcatctt tcatgatcca aaaaacagca tcgatctttt cgctaagatg atcgactcgg
    80281 tcgggtgtcg cataacgtga tttgactgtc cgcccatttc cgtttcgtat atctcctgtt
    80341 actaggactt tttctccttt acttgtacat gtcacgccca cattctgaca agtaaggaaa
    80401 gaagaaatgg ccggatcatc agacggatag tcttgtgttt tatcaaagta agaaggttcc
    80461 aaggcagtag caaaaccatt ttttcgagaa ataacgaaag catcgtcatg caagatcgaa
    80521 acagagtgac cttccgtatg ttttgctaag gtaatcgttg attttccaga accggacaac
    80581 ccaaatacag ccatcgtata ggtctggtca ttactagcat attgtttcat cccaccgtgg
    80641 caggcaataa agccattgcg atttcccaat gcccaagcaa gcgtcaaagt cgcttttttt
    80701 aattctccaa agtaacgtaa tcctagcaca gccgctgtat tatggagagc atcaaacaaa
    80761 atgagtccat ttggaaatgt ttcatgtgtc cattcgggat cacaaaagat ataaagatct
    80821 ccctcatcaa aggaaacaga cttttgataa gtctctaaga tcttcgaatt cgctggctga
    80881 aaattcagca aataagaata caaattgttc tcaaatcctt ctggaatcag caaatgactt
    80941 tgcacagaaa attcttcaga taggccaacg attacttttg tatggtaaaa ctggcgcttg
    81001 cgactttgat acacagcttc tcttaaaatt ttctcgtatt tgctttgttc cttgtctaag
    81061 tcctcaaaga tccgccgagc acttgctgtt cttcccacaa tgcttccatc attgaatact
    81121 agtgtcctaa catcagctgg taaatctagt tcttcagcat gtgagattgt taaatctgtg
    81181 atgatcgttc ctggactccc ttttgctagt tgataagctg tcttcctatc aaaaacttca
    81241 tgaacattat taccgtaaaa tgtcgtttct acaatcgatc gaatactgga taacagtgga
    81301 tttccacttg tgatttcgtt taattcgaga ctctcttttg tactcatggc tcctcctcat
    81361 tccctaaaca aaaaaacgtt ttctttatta tacattattc gagtttttat ttgtactgct
    81421 gcgctgtggg aagcaaacga gttatttttc acacaaaaaa accgagaact tgaataaaca
    81481 agtttctcgg tttcatcatt ttccttttat cggcttagta ccagccgttt gctaaccaga
    81541 atgttttagc agcatcccat gaaccataac gagatgttac atattgttct gctacacgtt
    81601 cttgattggc agctgaatag tcgccgttta agtatgaagc atctaattga taacggccga
    81661 tgtaacgtcc gttagtagct gtgtatgaac cgcctgattc tttttgtgcg atccattctt
    81721 ttgcagagct tgaagttggc gctgttgcag cagctggggc agcaggagct acttcaactg
    81781 tttcaacagg tgcaacgtat gtatgttctt gcgttgctgt ttcagttgta gatgctgtat
    81841 tttctgttgt tttgttttct gttgttgctg aagaatcatt tgaagttggg attgttaatt
    81901 gttcaccaac ataaatgcgg ttgatgtttg cgatcgaatt tttttcagca attgcatcga
    81961 tcattgagtt gtctcctgca aatttttgtg agatcttaga taatgaatca cctgatttta
    82021 ctgtataaac ttcatcagcg tgagcatttg tagttcctac aaacattgca gtagcaccag
    82081 cagcaaaagt tgtacctaaa acaattttct taaatgaatt catattaaac tccttttttt
    82141 ctccgttttt tattctttcg acgctattaa ctttatcatt tagaaatatt tcataggtaa
    82201 a
//
LOCUS       DACDUU010000004        80381 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.004, whole genome shotgun sequence.
ACCESSION   DACDUU010000004 DACDUU010000000
VERSION     DACDUU010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 80381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.004"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            148..1599
                     /locus_tag="IWL30_000242"
     CDS             148..1599
                     /locus_tag="IWL30_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP9705041.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1948..2580
                     /locus_tag="IWL30_000243"
     CDS             1948..2580
                     /locus_tag="IWL30_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAP9705042.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLTGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3168..4607
                     /locus_tag="IWL30_000244"
     CDS             3168..4607
                     /locus_tag="IWL30_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9705043.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4902..5120
                     /locus_tag="IWL30_000245"
                     /pseudo
     CDS             4902..5120
                     /locus_tag="IWL30_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5369..5584
                     /locus_tag="IWL30_000246"
     CDS             5369..5584
                     /locus_tag="IWL30_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705044.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5801..6057)
                     /locus_tag="IWL30_000247"
                     /pseudo
     CDS             complement(5801..6057)
                     /locus_tag="IWL30_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(6244..7155)
                     /locus_tag="IWL30_000248"
     CDS             complement(6244..7155)
                     /locus_tag="IWL30_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9705045.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(7174..8178)
                     /locus_tag="IWL30_000249"
     CDS             complement(7174..8178)
                     /locus_tag="IWL30_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP9705046.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(8175..10298)
                     /locus_tag="IWL30_000250"
     CDS             complement(8175..10298)
                     /locus_tag="IWL30_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9705047.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10303..12750)
                     /locus_tag="IWL30_000251"
     CDS             complement(10303..12750)
                     /locus_tag="IWL30_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9705048.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12737..13126)
                     /locus_tag="IWL30_000252"
     CDS             complement(12737..13126)
                     /locus_tag="IWL30_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9705049.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(13186..13689)
                     /locus_tag="IWL30_000253"
     CDS             complement(13186..13689)
                     /locus_tag="IWL30_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP9705050.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13702..13926)
                     /locus_tag="IWL30_000254"
     CDS             complement(13702..13926)
                     /locus_tag="IWL30_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705051.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(14030..15940)
                     /gene="ltrA"
                     /locus_tag="IWL30_000255"
     CDS             complement(14030..15940)
                     /gene="ltrA"
                     /locus_tag="IWL30_000255"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP9705052.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(16686..16805)
                     /locus_tag="IWL30_000256"
     CDS             complement(16686..16805)
                     /locus_tag="IWL30_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP9705053.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
     gene            complement(16820..18004)
                     /locus_tag="IWL30_000257"
     CDS             complement(16820..18004)
                     /locus_tag="IWL30_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9705054.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(18263..18553)
                     /locus_tag="IWL30_000258"
     CDS             complement(18263..18553)
                     /locus_tag="IWL30_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705055.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(18543..19685)
                     /gene="dcm"
                     /locus_tag="IWL30_000259"
                     /pseudo
     CDS             complement(18543..19685)
                     /gene="dcm"
                     /locus_tag="IWL30_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(19918..20592)
                     /locus_tag="IWL30_000260"
     CDS             complement(19918..20592)
                     /locus_tag="IWL30_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9705056.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            complement(20670..22010)
                     /locus_tag="IWL30_000261"
     CDS             complement(20670..22010)
                     /locus_tag="IWL30_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP9705057.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            complement(22093..22428)
                     /locus_tag="IWL30_000262"
     CDS             complement(22093..22428)
                     /locus_tag="IWL30_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP9705058.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            complement(22438..22812)
                     /locus_tag="IWL30_000263"
     CDS             complement(22438..22812)
                     /locus_tag="IWL30_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9705059.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            complement(22825..23139)
                     /locus_tag="IWL30_000264"
     CDS             complement(22825..23139)
                     /locus_tag="IWL30_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9705060.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(23252..26479)
                     /gene="fss3"
                     /locus_tag="IWL30_000265"
     CDS             complement(23252..26479)
                     /gene="fss3"
                     /locus_tag="IWL30_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP9705061.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYCQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            27267..27590
                     /locus_tag="IWL30_000266"
     CDS             27267..27590
                     /locus_tag="IWL30_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9705062.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            27754..28671
                     /locus_tag="IWL30_000267"
     CDS             27754..28671
                     /locus_tag="IWL30_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9705063.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            28664..29446
                     /locus_tag="IWL30_000268"
     CDS             28664..29446
                     /locus_tag="IWL30_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9705064.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            29447..30127
                     /locus_tag="IWL30_000269"
     CDS             29447..30127
                     /locus_tag="IWL30_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9705065.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            30120..31016
                     /locus_tag="IWL30_000270"
     CDS             30120..31016
                     /locus_tag="IWL30_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9705066.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            31174..33150
                     /locus_tag="IWL30_000271"
     CDS             31174..33150
                     /locus_tag="IWL30_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAP9705067.1"
                     /translation="MKIEIIIVHLSCIQEVKIMGIEFSERQMKILYFLIENEHTTYKE
                     LSEQLYVSTKTISKDILILKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKIN
                     IADSPEDRLTYVFAKLLNTNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLV
                     SDRNGVRIKADEKSKRRLMSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDI
                     LKKVAELLNRYMNKTQLKFTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNT
                     LILIKMIEKAFSIIVPIDEQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVR
                     IKLNEFYPDEELINNLVLHLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEE
                     IEERFCIHFNEDEIAYVTLHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFA
                     DMVEIEDVLSINELQDRKVTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQL
                     NEQTEDAFLNLLDKRFCFFTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKL
                     SSTAMNDFAMPHIEVDFIKKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKI
                     YETFNEIASNHELLANLKTSSSFEKLVYILKEGL"
     gene            33151..33597
                     /locus_tag="IWL30_000272"
     CDS             33151..33597
                     /locus_tag="IWL30_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP9705068.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            33613..33927
                     /locus_tag="IWL30_000273"
     CDS             33613..33927
                     /locus_tag="IWL30_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAP9705069.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            33953..35002
                     /locus_tag="IWL30_000274"
     CDS             33953..35002
                     /locus_tag="IWL30_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAP9705070.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            35014..37617
                     /locus_tag="IWL30_000275"
     CDS             35014..37617
                     /locus_tag="IWL30_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP9705071.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            37734..39383
                     /locus_tag="IWL30_000276"
     CDS             37734..39383
                     /locus_tag="IWL30_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP9705072.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            39699..40634
                     /locus_tag="IWL30_000277"
     CDS             39699..40634
                     /locus_tag="IWL30_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9705073.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            40627..41397
                     /locus_tag="IWL30_000278"
     CDS             40627..41397
                     /locus_tag="IWL30_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9705074.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMIFGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            41812..42795
                     /locus_tag="IWL30_000279"
     CDS             41812..42795
                     /locus_tag="IWL30_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAP9705075.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            43116..44153
                     /locus_tag="IWL30_000280"
     CDS             43116..44153
                     /locus_tag="IWL30_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9705076.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(44242..44565)
                     /locus_tag="IWL30_000281"
     CDS             complement(44242..44565)
                     /locus_tag="IWL30_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP9705077.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(44552..45259)
                     /locus_tag="IWL30_000282"
     CDS             complement(44552..45259)
                     /locus_tag="IWL30_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP9705078.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            complement(45558..46982)
                     /locus_tag="IWL30_000283"
     CDS             complement(45558..46982)
                     /locus_tag="IWL30_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9705079.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            47157..47870
                     /locus_tag="IWL30_000284"
     CDS             47157..47870
                     /locus_tag="IWL30_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9705080.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            48019..49350
                     /locus_tag="IWL30_000285"
     CDS             48019..49350
                     /locus_tag="IWL30_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9705081.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            49618..49770
                     /locus_tag="IWL30_000286"
                     /pseudo
     CDS             49618..49770
                     /locus_tag="IWL30_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(49915..50790)
                     /locus_tag="IWL30_000287"
     CDS             complement(49915..50790)
                     /locus_tag="IWL30_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705082.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            51388..52251
                     /locus_tag="IWL30_000288"
     CDS             51388..52251
                     /locus_tag="IWL30_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9705083.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            52351..52833
                     /locus_tag="IWL30_000289"
     CDS             52351..52833
                     /locus_tag="IWL30_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9705084.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(53096..53563)
                     /locus_tag="IWL30_000290"
     CDS             complement(53096..53563)
                     /locus_tag="IWL30_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAP9705085.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            53686..54537
                     /locus_tag="IWL30_000291"
     CDS             53686..54537
                     /locus_tag="IWL30_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9705086.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            complement(54869..56827)
                     /locus_tag="IWL30_000292"
     CDS             complement(54869..56827)
                     /locus_tag="IWL30_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9705087.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(56824..57696)
                     /locus_tag="IWL30_000293"
     CDS             complement(56824..57696)
                     /locus_tag="IWL30_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9705088.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(58038..58910)
                     /locus_tag="IWL30_000294"
     CDS             complement(58038..58910)
                     /locus_tag="IWL30_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9705089.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            59038..59607
                     /locus_tag="IWL30_000295"
     CDS             59038..59607
                     /locus_tag="IWL30_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP9705090.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            59674..60411
                     /locus_tag="IWL30_000296"
     CDS             59674..60411
                     /locus_tag="IWL30_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9705091.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            60642..61910
                     /locus_tag="IWL30_000297"
     CDS             60642..61910
                     /locus_tag="IWL30_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP9705092.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            62397..62861
                     /locus_tag="IWL30_000298"
     CDS             62397..62861
                     /locus_tag="IWL30_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9705093.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            62845..63474
                     /locus_tag="IWL30_000299"
     CDS             62845..63474
                     /locus_tag="IWL30_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP9705094.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            63828..65306
                     /gene="msr"
                     /locus_tag="IWL30_000300"
     CDS             63828..65306
                     /gene="msr"
                     /locus_tag="IWL30_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP9705095.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            65353..65799
                     /locus_tag="IWL30_000301"
     CDS             65353..65799
                     /locus_tag="IWL30_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP9705096.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(65998..66513)
                     /locus_tag="IWL30_000302"
     CDS             complement(65998..66513)
                     /locus_tag="IWL30_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9705097.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            66630..67832
                     /locus_tag="IWL30_000303"
     CDS             66630..67832
                     /locus_tag="IWL30_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9705098.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LCGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            68447..68773
                     /locus_tag="IWL30_000304"
     CDS             68447..68773
                     /locus_tag="IWL30_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705099.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(68890..69234)
                     /locus_tag="IWL30_000305"
     CDS             complement(68890..69234)
                     /locus_tag="IWL30_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705100.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(69310..70557)
                     /locus_tag="IWL30_000306"
     CDS             complement(69310..70557)
                     /locus_tag="IWL30_000306"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP9705101.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            complement(70554..71360)
                     /gene="proB"
                     /locus_tag="IWL30_000307"
     CDS             complement(70554..71360)
                     /gene="proB"
                     /locus_tag="IWL30_000307"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP9705102.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            71590..72117
                     /locus_tag="IWL30_000308"
     CDS             71590..72117
                     /locus_tag="IWL30_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAP9705103.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            72180..73550
                     /gene="radA"
                     /locus_tag="IWL30_000309"
     CDS             72180..73550
                     /gene="radA"
                     /locus_tag="IWL30_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAP9705104.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            73584..74741
                     /locus_tag="IWL30_000310"
     CDS             73584..74741
                     /locus_tag="IWL30_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAP9705105.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            74911..76371
                     /locus_tag="IWL30_000311"
     CDS             74911..76371
                     /locus_tag="IWL30_000311"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAP9705106.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            76693..77229
                     /gene="cysE"
                     /locus_tag="IWL30_000312"
     CDS             76693..77229
                     /gene="cysE"
                     /locus_tag="IWL30_000312"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAP9705107.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            77226..78638
                     /locus_tag="IWL30_000313"
     CDS             77226..78638
                     /locus_tag="IWL30_000313"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAP9705108.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            78635..79030
                     /locus_tag="IWL30_000314"
     CDS             78635..79030
                     /locus_tag="IWL30_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP9705109.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            79023..79883
                     /gene="rlmB"
                     /locus_tag="IWL30_000315"
     CDS             79023..79883
                     /gene="rlmB"
                     /locus_tag="IWL30_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAP9705110.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            79887..>80381
                     /locus_tag="IWL30_000316"
     CDS             79887..>80381
                     /locus_tag="IWL30_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAP9705111.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLS"
BASE COUNT        25182 a        14896 c        15808 g        24495 t
ORIGIN      
        1 ttaaaggcag taacaggatt aaacattgaa acataatcgt tactaaatga aatcaagaaa
       61 tatactgtat tactcaattc attgaaaata tttatcataa tttttaatag aaaacactat
      121 taaagagaaa aaagagaggt aaatcaaatg aaaaaatctt taaaacttgc atcagcagtt
      181 atattaatgt cccaactcct tatagcaaca cctttccatg tgttggcaag cgaaaattca
      241 acatacaaaa ttgcaaaaga aagaaccgag acgactgaaa tgagtaattc cgatgtaaca
      301 gcagagagta gtgaactttt gacaacttca tcaagtgaaa gtagtacgac ttcaaatgcc
      361 gagtcttcaa caacttcatc aagtgaaagt agtacgactt caaatgccga gtcttcaaca
      421 acttcatcaa gtgaaagtag tacgacttca aatgccgagt cttcaacaac ttcgtcaagt
      481 gaaagtagta cgatttcaag taccgaatct tcaacaactt caagtagtac aagtgaaata
      541 acttcttcga aagtgacaaa accaaaccat atagaatctt caaaaaagga agcgccaaaa
      601 acggaaggac aaacaaaaac tttttcgaca ccacagcaaa tagaatcaac agaatctttc
      661 caatcacaac caatactatc tggtaatgaa gaaattcatt ttgacaaaaa tgaatctgta
      721 gaaaatttta ttatgaaaat tggtgagtct gcgagaaaaa ttggtcagaa atatgattta
      781 tatgcttctg ttatgattgc acaagcgatc ttagaatctg ccagtggtca aagccagtta
      841 gctcaggcac cgaattacaa tttatttggg ataaaaggaa cttataatgg aaactttgtc
      901 attatggtaa caaacgaaga tttgggtaat ggaacgcttt atacgaccca atcaaaattt
      961 agagtgtatg aaaactatga ggaaagcttt gaagattatg ccaaattatt aactaaggga
     1021 attagtggaa ataaagattt ttatgcagga gcattaaaag ctaatagtaa aacatatcga
     1081 gaagcaacta aattccttac aggacggtat gcaacagata cacaatacta tttgaagcta
     1141 aatgaattaa ttaaaaccta cgatttaacc aactatgata aagaagttaa aggtccacaa
     1201 atgagtaaga ctggatacca agtgcctatt cataattatt caataaccag tttgtttgga
     1261 aatcgtggaa atgaatttca ccgaggactt gatatggcag ctacacaggg tgagccgatt
     1321 catgcagtta aagctggtaa ggttgttaaa gcagagtttc actactcttg gggaaattac
     1381 gtggtgatac aacacgaaga tggatcaacc gcgttatatg ctcaccagca acaataccta
     1441 gtaaaaactg gagataatgt tttgcaagga caaataattg gctatgtagg aagtactggg
     1501 aatagtactg gcccacacct tcatttggaa atttgcaaag atagtagtct ttctcagagc
     1561 atgttagttg atccgaaaac cttattattc ggagactaaa aaaggtaatc aatctgacaa
     1621 agtaacaaga aaatggttgg attcttagac ctttgatcag gatattgaga ctggaattga
     1681 tatctcgatt acgatgggtt tgacaaataa aacaagtcca ttatcgaata tcgagtgtat
     1741 tattgccgtc tttatgctca tattttgagc aggtttcact agatgaaaac aatttatcta
     1801 tcttgaggat ttcgtgtacg tatcgttcag tcttgtattg taaattagtc ataaaatcag
     1861 actaagaaaa attggaaata tttttttctg atttattaaa agttaattga aattatatgg
     1921 tacttctttc ttgggagggt aggtgcagtg aaggaaagat atggtcacct tactgttgtt
     1981 gaggagatta ctcataatgg atataaaaaa tatcgttgcc tatgtgactg tgggaatgaa
     2041 acgattgtat atcgatcaaa cttactaaca ggtagaacca aaagttgtgg atgtggaaat
     2101 tataaaaata ggcataaata taaaaatttg acaaataaaa cttttggtaa gttagttgca
     2161 atcaagccaa ctaaactgcg aagtgaagga actgttgttt gggaatgtca atgtctgtgt
     2221 gggagaaaaa agttagtatc ggcaaagcga cttctaaaag gtgatgtaaa aagctgtgga
     2281 tgtttgaaat caaaaaagca tcacattgaa aataaaaaat tcggattact tactgcaaag
     2341 tatcctttgg acaataactg ttcatggaaa acaaaatggg tttgtcattg tgagtgtggc
     2401 agtatgtgtg agataagtta tagtaactta ttttttggac atactcaaag ttgtggatgt
     2461 ttaaaaaggc aagaaacaag gacatgtgtt gaagggacag taatagagtg tctaacgtca
     2521 aaattaccga aaaataatac aagtggtgtt aagggagttt atcgttctaa agagaaatag
     2581 attgcgtata taactttaaa aaaaaagagg tactatttag gatcgtatga ttctattaag
     2641 agtgcagctc aagctagaaa agatgctgaa aataaattgt ttcaaccttt gattgacaaa
     2701 tatcgaggta atcattaatt attgtggata ataattaaca gtgatatata aacatctata
     2761 atatattatt catactattg atatttagta taatcctatg gaattgaata tcatagcaat
     2821 gtatttgaaa agcaaattgg aaaacaaata ggtgcaacac cataaatatg aaaaaattgg
     2881 gaaaaagtag aatggaaatt tgcttacgtc tcacgagata gctcacacta ttaatctgaa
     2941 gaacaatgaa gtcgactttt agaaattgct agaagaaagt aaagcgaatt atcactagtt
     3001 actatagagt aagttcaaat aaagcatctc caacttacat ggttacatga ggatatcaaa
     3061 tcgaaattac tcaagcttcc tatggctaga gtcgcgagaa gttgttgggc agtagacata
     3121 ggttatattg ttcttagcta cacgatcatt agaaaggaaa tcctattatg tatagttatc
     3181 aagaagatgc aaaagtccaa aaaaatcgct ggtggatttt gattgctata tctatgttta
     3241 ccttcatgtc cacattggat ggcagtattg tcaatattgc actacctacc atctcagatg
     3301 atatgggcat accaatgaat caagcggaat gggtagtgtc cgtttatctg atgattgtct
     3361 gttcttgttt gttgctgttt ggtaaaattg gcgatagccg tgggaaaatc aaagtatttc
     3421 ggattggtac gctgatcttt gtcttgggat cgctgttgtg tgggttcaat cattcgctag
     3481 gtattttatt gttcgcacgg gtggtacaag ccattggtgc tagtatgact atggctacaa
     3541 atgccggaat catcacggaa gtctttccca tacatgaacg tggtcgggca ttgggagcaa
     3601 taggtgcgtt tgtttcatta ggctcgattg ctggaccagg tcttggcggc ttgattttgt
     3661 cccaattttc ttgggcgtac attttttgga tcaatgtacc ggtgggaatc gtaacgatgt
     3721 tgattgctga aaaattctta ccaaaagata tcaccatgag cggccataca atcgatggag
     3781 ggggctttgc tgccattgcg gctacgatta tcagcttttt tggagcaatc tttttggggc
     3841 aagaaagtgg ctttttccat ccagtccccc ttgtactgtt cgtaatttca gtgattgcct
     3901 ttgtcagctt tttaaaagtt gaaaagaaag cagcctctcc attgatttct ttcggtattt
     3961 ttaagaacaa gatttttacc atgagcttga ttacagcctt gttgattttt tcttccaact
     4021 tctttgtcaa tgtagtgatt cctttttatc tgcaaaatgc tcgcgggttg agtcctagct
     4081 atgctgggct gttgatgatg gtctttcctt tgatgatggt cgtcggggcg cctttaagcg
     4141 gctacctgac cgaccgggtt ggcccggaga ttttggtatt agtaggcttg ggcttattgt
     4201 cggtaaccca ggtcatgtat atgtttttgt cagtggactc tcctttgtgg ctatacattg
     4261 tcgctactgc catcatgggt gccggcaatg ccttgttcca gtcccccaat aatacgatgg
     4321 ttatgagctc ggtcactatc gaaaacttgg gactggcagg ctcgatgaat tcctttgccc
     4381 gaaatttggg gatggtcagc gggatttcgc tttctacgac ggtcttatat caagcgatgt
     4441 ctgctaagat gggccagcaa gtaactaact atatcgcaaa tcggccggat gtctttttat
     4501 atggaatgaa gattaccttt ttaggcagct ttgttttgtg ttttgttgcc tttttcctga
     4561 ctatctggcg tatcaaaaaa caaggaggta ttcagaaaaa tgtctgaaaa aaccactgtt
     4621 gaagaatttt tggaggcctg cgtatgggtg agtgggattt ccatggatga tggaagatga
     4681 agtgaacttc taaaagccga aaaactcaag tattatcttc aaaatactag tgctcaatga
     4741 gatgcttgat ttgatcacaa gaagactgcg aaagccgctg gcaaccgcaa gctaggtatc
     4801 ccaccgatgg actacgtttt agaggctact ggttatgaat acggagccaa cgctctcatc
     4861 tgcataagaa tacatcaccc agattatgta aagattttcg atacttctgt caaaaaatca
     4921 ttaagataga taaatgattt ccgtctagtc aaattggttc agaatgtaga cacacagatg
     4981 gtaagaagtc actcacaatt cgagaatgga cttgtcctat ttgccgtata catcataatc
     5041 gaaacgtcaa cgatagtatc aatattttga ccaagggcta tgaatacaag cattgcctta
     5101 gaaaacatca agaaccttag gaacaacggg gatagcttgg ttaataagag ataccgctgt
     5161 ttaggcttgc aagtatactc ttttatcaag aattccgtgg ctttagtcat gagaggctta
     5221 atactatgaa aattttcttt agcatttttg atttaggttc cttttctaat tataataaga
     5281 tgaccactac agtaacctac tgaaatatat aaacagccac tgtagtacca ttttcaaagt
     5341 agtctatgag aggagaatct tcattcttat gaacgctgga attgcggata tgaatttaat
     5401 aaaaaaaacc cttaatgatt ttacttccaa ttcaatatca aaaggtactg gtatcaattt
     5461 aagcacaata aaaaagctga aaagtggtga aagatcagtg gaaaagctga atcttttaga
     5521 tgctataaaa ataacggaat ttgctatgaa aaatggaaaa gctgagattg aaatatggag
     5581 ataattatcg tctagtgaat tacttgccac ttagctgaat ttaacgatcc ttggtgcttg
     5641 aagggggaga ttcttgggaa tagagcatac ttgcaagcct atgtctttac taacaacgat
     5701 atctcttatt aaccaagcta tccccgtagt tctaaggctt ttacttttat ctaagcaata
     5761 atcgtattca aaggaaattt ttgaccctta gaaagtctca ttttatcaga cgtgagctta
     5821 ggcattgctg aattttgtta attcggtttt aggttgtgct ttgagcataa tatgaaagta
     5881 ttctttatca cgatttcatt catctaaggt aatgtgatgt gattctgaaa ttctttcaaa
     5941 ggcagttttt gcataagctg acattacatc atcaatgaca tgtttacaat gtttcactac
     6001 taaaataagg gggtaaccaa gaagaaaaac tgaatgatta tttgtatcta attccatggt
     6061 atatcaacca tttcctatat agactgatta tgacatggat taaaataaaa aggcactatg
     6121 tcttaattgt cggttttcga gttaccgaat ggcattcatc acccacctat agaggatggg
     6181 cgacttctgc ctgaaataag ttacactact tataacttct aataaaatta gattgagtca
     6241 ttattattta acatcaacga ttttccagtt tccatttttt tgtaatacta atttgtactg
     6301 aaagtagcta gtggtttttg atacattatc aaggtatttt atctttgtac tcactagaaa
     6361 attttttccg ttttgtagat aaactggatt ctctaactcc gaaaacttta agtcaatatt
     6421 gattggtcgc atcgcaccat tttcaacata atactccaat tctttttcag aagctgatgg
     6481 ataaagttta aaaaatgttt ctaaaaaatc ggtgatttct tccgttgttt tggcatcaat
     6541 ttctgaatcg ttttctactt gttttgcttc atattttgcc ttgtctggct ttccaatcat
     6601 tgttggcaaa ctagttacta aatgattcct gttttcatct tcaaagattg tcactcgata
     6661 tgcagaagaa attgattgcg tcttttttcc ctctgtaata tgttgctcca ctaaaaatga
     6721 aacgacaaat tcttccgagt gtttttcaac atctagaatt tttacagatt gaacttcgga
     6781 actcgtggga atatctgctc gaaccatatc ttggattaaa gcgagaccgt tttctgtaag
     6841 gtactgttcg agattactca ttctcttttc aattgcttct ttgttatttt tccatgaaaa
     6901 atattctttc gcaaaatcct ctgtaaaact ttcaacgcca gaagtattca ctaccttctc
     6961 ttttataacc actttttcat gagttgtatg ttgatcaatg gcggtgaaat ttttataaac
     7021 gccaaaagca agactacaag acaataaaag ccaaagggca agcaccattt ttcgatgctt
     7081 acccacggat acaactcgtt cttttttctt ttttgggctc ttttcctttt gattccgttc
     7141 aatttttatc ttcattcttt ttcctctttt ctcttatttg tatcggccag ctccgataag
     7201 atgctgccgc caatagtttt ccgttagatt cgtccacccc actggatctc ctgcatgaaa
     7261 catgcgattc tcccctgcgt aaatgcccac atgggtcaca taagtaccgg catcataggt
     7321 tgaatgaaag aaaactaaat cacctggttt ggcatctttt agatcaatat gagtcatggc
     7381 atcatattga tcctgagcca cccgaggaag tttcaggtca actttggcat aacaccattg
     7441 ggtcaagcca ctacaatcaa accccgtttc tggacttgag ccaccaaaca cataggccgt
     7501 tccttcatac ttaaatgcct catcaaaaat ttcttggact attttgtcat caaactgagt
     7561 tgtagttagg tattgcttca ctaataaaca gtaaaacata ttgccataat tataccgcca
     7621 cccaccattt tccttaatcg cgattgggtt ggggtaatcg actttttgac cacctgattt
     7681 ttcctttgaa aaagattcag ctaaagcaaa actatgcttc ttcccgtttt tcccgacatg
     7741 atctagatac ccgccaccat agttataggc ctgaataata ctatcttgat cacaccctag
     7801 ttctttggaa cgcttaacca aagacgcaaa atagagacag ccttgtttga tggatgcctc
     7861 ggtacttaat gaattaggtg gtaatccggc tgactcagaa ctttgcataa cgtcttccat
     7921 tgtgccacca gattccactt gaataatcgc caaaagaatt ggaatatact ctgttatatc
     7981 cgattctttg gcatactttt ccaccattgc acggtgtttc aatacttctt ctgagacact
     8041 agttccgcca ggagaagtgc cagaactacc atttccctca ctgtcttcac caaacaacaa
     8101 acccacacac atcaaaatac ccataaaaga gatggtcaga aaagttaatg aaataacacc
     8161 cactttcttc aagttcattt tttctcacct gaccttgatt ttggacgatg gttcattctt
     8221 ttttgtactt tttgattggt ctgctttggc agagtggacc tctgtttcac ttgttggaaa
     8281 gaatttttct tacttgctga ttggcttttc ccttttgaaa tgatttcttg actagttagt
     8341 tttcgtttga tttcgggatt cagatttgct ctcacaatcc gctttggtgc ttcttttttc
     8401 agcatcatat ctttctgagg ttgagctgct ttatctattg gaatattcgc ccttcttttt
     8461 ccctgacgat ttgttgcttc actcagtatc ttgcgttttt cgtccatttt cgcatttctt
     8521 cgttgtcgtt gtttttctcg caaatccact cgatccgctt tttcttggaa cagtccgcgc
     8581 ttgaattctt ctggtgcttt tttcgacttt tcgattcctt tatggagatt gtatttcaaa
     8641 ttcgttggcg tattccttac ctgatccttt acttgtttag tcttatcttt caccaaagct
     8701 tgggtatcca aaactttgcc gatttttctc ccagccaaat tcattcggga ttgtttctta
     8761 ttatttgaaa ctggattctc cttagttaag tcagatagta tttcgtgatc gttcggtaat
     8821 cgtgaattct ttcgtagaga agaaccggat ggcttaaatt tggattttga ttgttccttt
     8881 gccaacttat ttccgactgc tgcaccacca ccaaaagcca aagctcgtcc cacatttcga
     8941 ttgagttgcc gcaattttcg attcattaac atttgcggtt ttcgcatgac gcgtcttccc
     9001 attgactggc tatcattact ctggaggttg aacatactca taatgtcgcc caatttgaaa
     9061 tagataccgg caaaggtcac aatttgcaga aaagcaacca tgaaaaatgg ataggttgcc
     9121 gaaatattga aaaacatcgt ggagatgcta aatgcagtcg taatcaccaa agtaacaccc
     9181 gctcgcatca taatcgtatt gaataaacga ataatcgcct tctttcctaa actttcataa
     9241 gtcggcaaca tggacaataa gaaactaatt ggcagaaaca tcgcaaagat aatgaagaga
     9301 atttgcgaga acaacataat ccctgttaga agaaaaacaa agaaggaaat gccaatattg
     9361 aacaatacca aaaagaccac ggtccctaaa cggttcatgg tcttggtaat actgagattc
     9421 gcattgtcat tgtcttcaat ctctgccttg accgcttctt cccgatcttt gcctttgttt
     9481 tcatctggac tcacagatag aatcttgttg acccggtctt caccaatttc ttctatgttg
     9541 gaatcatcaa attgcagtag taaccaaggt tgttggactt gaatcgaaaa caaactatcc
     9601 cgaatcaaat ccacactatc ttttccttgg ctttctgaat tgggtacaac gatctcggtt
     9661 ccaagggtaa gggccgcttc actaacatct gagctaaagt cattgatttt tgtgatatag
     9721 gtaggcgcat aagcaatgaa cgaacccgat agtagaaaaa tcatcacaaa attaatcacg
     9781 gctcgaactg ctttcgtggt ttctcgtttt aataatccgg tatacgccac atacaccccc
     9841 agagctaaaa tcatcaataa tagaaaccca aaataaaatc cggaggtctg gagtccattt
     9901 tcagtaatac cagccaatgt ttggatattc tttccaatac tttcagccgt atccgaaatg
     9961 aaatctaatt tgtaagcctc ttgaaccaca taacccgtgg cgttcgacaa atacaaactc
    10021 accgtccaaa tgaaattagt aatggcataa agaccataca tcacgctttt ccctaagcca
    10081 tcaccccaat tccaaggcaa ccaatcccaa gagctatcca caaagaaatc tagttgatag
    10141 ttgttcagtg aatatttgga atatagattt cctgcttcaa ctgtatcatc aactagccct
    10201 gtcgcctcgg ctaccgtacc aactaaactc aatagaacta tcagagtgag tatgctaata
    10261 aaaacgattc ccacaatccg aaaaagtttc tttttcatcc cctcactcca ttctctcttg
    10321 aacaggtgga cgggtatcaa aggcatggaa gagttcttca aagactggat gtacctgaac
    10381 cacgcctacc cgaccataca aatcctgcat caagcattgg ccattttcca aatctcgtag
    10441 gcgtttttga ttgctttcat cttctcgatc caagccaaag aattccaagg ttttcttgat
    10501 ttcatgaata tcggtactgc gaaacgcaaa cttcaacccg atattattct tcaagcgttc
    10561 atccagtaaa tcatcactat tttgcgtgac aaagtaaacc ccggcattca tagcccgccc
    10621 agcacgaacg agcttattcg ataatgtttt cccttgcgct acttgaagaa aactccacgc
    10681 ctcatctaag tctacaattt tgaagatttc tcgattacta tggataaagt ccaaggcaaa
    10741 agtggaaatg acgattagca tggaaacact aagcaattcc atggtggtat attcttcaaa
    10801 agtggtttct gcatctggta aaaccaaatc cgctacttga atgatattta actgcctatc
    10861 taaactaatc gactgggtaa tcgaaccatc tgagaaaagc aaatgggcaa aatcataatc
    10921 ggtaaaggac tcgatgtgat ccgcgatatt ttccgcaact ggtgagcctt cttttcgaag
    10981 ctcattaatt acttgtaata atccccgttg attgctttgg gtaaccgtcc gaatggcctt
    11041 acgcaacact ggaaacttct ccccatctcg actagaaatt cctgttaaaa aggtcagaat
    11101 atcaattgct agactttcag aatccttctt tttctccata atcacataag gatcaagtaa
    11161 tccttgattt tttggcttac tagttaagtt gagaatattg atttcatccg caatttctgg
    11221 caaggtttct ttccagccac cccgttcccc ttttggatcg acaatcacag cttgtccacc
    11281 aaaaaggacg gcgtaataaa ctaagagatt gttactaaag gactttccgc caccgagtga
    11341 accgaggaat gccgctgcta gcgcattggt aactgaatcc ttgacccctt gtgaggccaa
    11401 tgccggtttc aggtaaacat tgcgccccgt atctacgtta taaccaaagt aaatgccttc
    11461 caactcccct aacatctgag tggcgccaaa accaagtccg gcgagaaaat cagacgtaac
    11521 atactggata taatcattca tgtatcgctt actagaaggt aagaattctt catgtagccc
    11581 catcatatcg ccaaaaggcc ggactagttt tacattggta tcatcataaa aatctagcac
    11641 ttcatcacac cggcgcttta gttcatctac tgattcggca cttactcgaa ccacatagct
    11701 cagtttgtac atggattctt tggactgatc caaggtagtt tccaattcat ctacggaatc
    11761 caatgcatct aagacattgg aatttgtttc attgtcagat tgataggcat ggttgtccaa
    11821 gtcttttaat tcctttttct tattgcgaac ggtagcaagt gcctttttgt tcgtcacaat
    11881 ctccacattc atactagtgt caattgggaa tgtaaattgt tgttgctggt aatagaaaag
    11941 ttctgaggag ggaaactcca tttcccccac aattgtatta attgttaagt acgctacata
    12001 tgattcatga ttctcatgtt ccatgcataa gtaccggggc ttttcttcaa tcaaacaacg
    12061 actgggacgt aataagtcgt aacgtttcac caaagtttcc gacttcagct ttttctttgg
    12121 tagctgaaat tcatattctt caaatggtgt acccttttcg ccatagatat gttcaatcaa
    12181 atacatcaga tcactcggtg tgactcgtct gactttaaat cttcgagcca atttactttc
    12241 cattagtttt tcaagcttgg tatatcggcg aatttcttca ttggataagg aaacaaaatc
    12301 gcccatcaaa tgatgattca ctccatagac aaattcttga aagccggaga agaaagattt
    12361 cttgagattc tttagattga cctcttcatc tgtcgcaatc agtttgaagc caataaaaaa
    12421 gcgataatca atctgactat cgccaatcat ggatactaat gcctctgtct gtaaatcaat
    12481 tcgttgtttc gctacttctg ttaatcgccc ggtaatttcc ttttttgatc gttcttgcgt
    12541 ggcacgaata ctactttctg tggcaatttg taaggcatga atcttgcctt cacgattttg
    12601 cgcaatcaat tgacgaaagt tatcgtggac ttgatatttc tgttcaggtg acaaaaagga
    12661 gtaattgtaa ggtattaact catagtaggc aaaacattct ccctcttgat taaagacaag
    12721 attattttca atatacttaa tcgggtacat agttcacact ccttaccaga gtaatgggtt
    12781 cgtcccatcg tttcttctgt tctttgactt tctttcctgc gaaagtgacc ttaaatcgta
    12841 agtgatagct aataaaagat cgtaaaaatc ccaatggttt cttaccgtca aacgtttttt
    12901 gcgacatgaa ccaagtaact gccaccggta tacctaagta ttttaaaaag gccccctcta
    12961 tcatagatag tggaggcaca tgggcaaata cgatgacaaa cagcaaggat aaaacaaacc
    13021 aagccatctg gttaaaagtc acaggaaaag gcagttgaaa gtcattaatt gcataaatga
    13081 ccttttcaac tgcccaaata ctggtgtagc tttttatttt cttcatcaaa tcattccttt
    13141 aaaaagaagg atagtttaga aatcaactac ccttcaataa ttttgttaat atggatactc
    13201 aaaaacacct cgactcgtta ccaaataatt gcctccaatc tccaagtctc taccacacgc
    13261 atcaaaatca atatacgtct tcaagtgttc tggtacttct ccaaaatcac cagcttctac
    13321 aatcaagaaa tatgctacat cactcatatc atcgcaatct ggataataga taatatcatc
    13381 cacatgctct accatctcct caaaagaatt gaagaaagca tgttgaatct cagaagcaac
    13441 ttcaccaact ggcgttcctt caagttcctc cgctaagcca cacaaccgat taatttcctc
    13501 aattggcgta tactcatcaa tctcaaaggg aagttcataa tcatgaattg catattcctc
    13561 gtattcgtca tttaacccaa ttcgttcttt cacttcatca aaatcgacag gtggggtaaa
    13621 ccacgccccc accaattcac cttcgttata tttcccgagg ttcgcaatat aaacacgcat
    13681 ctgttccatc gtgcttcccc cttacgcacc gacaattcgg ttaaacaaac tgagtaagac
    13741 atccttcacc ccagaagcat taaagaccaa tccgaccgca atcaaagcaa tcaccaaaaa
    13801 gccaatcagt ttggaaaact cccgcttaaa tcctaaaaac aaaccaatga ccacaattgc
    13861 cagtaagact aaggattggg cattggttaa aaaccattga tatatggata ggtaagttcc
    13921 tgatattata aatatctcaa acttttcccc ccattcacac ggagcgtgcg actttcaccg
    13981 cactccgcgt tccatcgtgt ttatattgaa aagtatttat gtagtagacc tatatcaagt
    14041 ttaattcttg attgccagct aatactctat atttatttag tcttatgagc tggactttat
    14101 ttagcttcaa tagagctata tatttttgta ttgtttcttc tgttttagcg tgtattagac
    14161 ggtgtaccgc ctcttttacc aaaataagat ttttataatc gtcatttcca ccagctccag
    14221 ttggtacaat atgatgacaa tgtatttcat tagcttgttg aaattcttcc agagttatag
    14281 cacattttcc atgttgagca gaaaatagcg aaattcgatt gtctgcgtat tctattgtgc
    14341 gattttccaa agataatttc attaactggt acatcagtaa tttatcaaca cttagattgg
    14401 tgtggataaa ttttcttccc tctagagtgt agcaattcgc taaatacacc tttgccattg
    14461 gttttctgtg ttggatatat ccaacaggga aaataggttc gccctcgcca gcaacatacc
    14521 gaagcatttt agatttacca tatcttgttg cctcctttgt cgtaagttgt ctgcctgttt
    14581 ttctcaatct gccccttttg tttgtaccta atctgttagt gaagatagcc attacagctc
    14641 tattcagttt catacaatcc agatttacat tcgttgcaat ctgataataa ttttgcatac
    14701 ctaataccat tgaattataa agtcttgttt cttctaattc cgttttccct tttgagggag
    14761 aagcaatttt ctttgcctgt gataccagct tgtctttttg gtttttgaat tgtttatcac
    14821 ttatatgtga tttaacaact aacttctttc ttctcggtat cattttaatc ttgaaaccaa
    14881 gaaagtcgga gtatcttttt ctaacattta caattcttgt tttttcttgt gatacttcca
    14941 attttaatcg ttgttcaatc cattgagtga ttgctatttt ggctttttca gcactttcct
    15001 tgtagcggca gaaaactcta aaatcatctg cataacgaac catatacatt tcctttaact
    15061 ttgttttctt catttctttg taagcgttgc ttttacatgg gctaccgctt ttattgatat
    15121 gaaccacata gttttttgta acaggatttt cctgccattg gctttctacc caatggtcta
    15181 actcattaag aacaatgttt gctagtagtg gggatattat tcccccttgc ggagttcctt
    15241 tctctggata tgttattgta ccgttagtca ttttgattgg agcttttaat atccttttta
    15301 aaatgaatat caagtgcttg tcccgtattc ccatagccca tatctgtttt atcagcttgg
    15361 aatggttcac gttgtcaaag aaacccttaa tatcaaattc aatcacataa tgtagctggc
    15421 tgatttgtag gcggttatag gttgctttta ttgcattttc aactgaccta ttaggtctaa
    15481 aaccatagct gttttcacta aatttagctt cgcagattgg ttctaatact tgttttatac
    15541 attgttgtat aagcctgtcc cacatacaag gaatacctag aggtctggtt ttaccatttg
    15601 gttttggtat ttcttttcgc ctcactggct taggtcgata cccatgtgga ctgcctgcaa
    15661 caatgtatct tacttttttg acaactgttt ctgctggcaa tttgccaata tctttaatta
    15721 ttgtaccgtc tgttcctgct gttttactgc ctgcatttgc tttgatattt cgatatgcca
    15781 gtaatatatt ttctctcgat aggataatat ccataagatg agtgaaaata tctccattct
    15841 tacttttggc atataaatca tcaaaggtgt tttgcatttc gtaatattca gcgtgtctta
    15901 aatcttctac acacaatgtt tcatttttat ttttaggcat aaggcttcac tcctttactt
    15961 gaaattgagt gagctttttt atcttactcg taatccttat tgaaacaaca taatactttt
    16021 ctaatatata cacgacttga gggcattcct ccattctcat tacaagaatt tcttaggttc
    16081 gccctctgct tttcagcatt agttcaactg cttattgttc ttcgtcagtt tattctcttg
    16141 atgagaaccc aatacctttc ctcgttccga taatcttatc tatacatata tatccttagg
    16201 tcactagcta taagcctgct ggcttgatac cgcctgtaac gttatatggc atttcataac
    16261 ctatattctt actttgccac accagctacg atttctcgta cctttgcatt tctacaaagt
    16321 tcaaatttag acccgtacat tcgctgtttc gtcagttcct tatagaacat tctaaccata
    16381 gatattttat agacccccga cctgttggat acgctaccca cctctggata gttttcgata
    16441 gaagatatat gttcatcaac tattacggta tctttcagcc gtctttaccg agcttcaaac
    16501 attctgagtt tcctcataca atgcttgtcg gaggctcagg gcaggcgttt cagagcgtta
    16561 cttcatcatt ccacctgtca gattatcagt tctcctaata accaaacttt ttcgttcttt
    16621 tattagtgcc ttagcttttc actaaggaac gagtcgcaca taaattttgt ccgaaattca
    16681 tcttgtcact ttcctttctt ttccattgct cgatccgctg ctgcacttgc attcatcaag
    16741 cacatcacag taacaccaag catactgcca ccaaatacca aaaaaatatc ttttacaatt
    16801 cccatgacga ttcctctttt catataatca aattttcaat tgccatactt tgttgctcca
    16861 aaatcttttt atgtcggtct gataagcgtg cattctgtac catatcctgg atataggttg
    16921 tccgattcaa tcgatccaac tccttagcca ttttccacgt aggggcgact tgtcggccta
    16981 gccaattcaa ggttcgttca atggtatacg gttttggctc ggaagttaat tgaatctctt
    17041 tccgattccg tccaataaaa cgcccccatt gttcattcaa gggccagttg gttcgccttt
    17101 taccttctac tttatccgca aatcgcaagt aacgattgat aatggaaaac gtggtgctct
    17161 caatgctacg accttttaac aaatcttgaa tcgcatgaaa agcacggtca tttttcagtc
    17221 gaatctcaaa acgatttttc gtctcagttt ggtctaatgg aatccctaac ttgatatatt
    17281 gttcatagtc tttttcatac aaacagaaat aaacctcact cttgagactg ccgatatata
    17341 aggtattgcc catgccgtcc ttttcatcag cctttaaaag ttccccggaa cgataacttt
    17401 taaaggtacg aaacaaggag atacattctt ccttctgaca ttttttcgtt agctcaggta
    17461 tatccaatag ccccactcga tcattgattg ccaagtccaa gcgtttcatc acgccaccgg
    17521 cctttagaca atcctcgaaa aagtcgtacc agctacgctt ttgagcgagt aaaaaagttt
    17581 caaattgtcg gcaacctcgc ccctttaatt ctaaaagcac acctttatct tcttcatgag
    17641 acaacatcac cacaatatca cccatgacat actgttcttg ataggagtaa aaggcataat
    17701 cttcatggag catatactca aatttcaaat ggagaatttc ttgaataatt tttcgtgcat
    17761 ccgttgtggg aaagcgaatc cttacatagt caaacagtaa cgtcaaaggc aaatcgggat
    17821 taaagctttc cagttggtca aaaattttcc tcatggtttt tatagatgcg gttacgccac
    17881 cattttctat ctgatttaag tacggccgac taagtccaca agccgtagcc aatttatttt
    17941 gtgaaacgcc gtattcgttg cgtcggtctt ttaatagccg ataatctaaa ttccgttgtg
    18001 ccaagttctc acctccaaat tcaaggtgtt aactttttca aatgagttaa cacctataca
    18061 aattacttac tatctttgat acaacaagca ttcttaatgg ttgcttactt attttttgtg
    18121 tttttgtacc cccctgttag atacgggggt gagacatcat ggtcggctaa cgccgtccat
    18181 gaacaggcac agcaccacgg aggatttctg cttcgcgaat cctccgcggt cagcgcctaa
    18241 cagtcagttg tgttggttta ggttactcat ctgtaatacc atttacttcc aactctgctt
    18301 tcatttctaa caagtattga cgaatatctg gttcttttgc ttggtccgca atccgctcaa
    18361 attctgcaat tacttgattt atttcaattc gagcatccgt ctcctcattc attaaatatg
    18421 gatcgccagc gactttgaca ccattcttgc gaatatcaaa ttttaaccgt cgcaattgtt
    18481 cttctaattc tgggtctgac tgaatctttc caatcaccca ataaatttct ccaatcaact
    18541 catcataaac catgtacatc caactctttt aatcttctgg caatatcaaa aatgaccgga
    18601 actgttacag agtttccggc ttgcttatac aactgactat ctgaattgac ttgagaatct
    18661 cgatcaaaag cccaatcggg aaagccctga agtcgccaac actctctagg tgttagcttc
    18721 cgaatttgat actgatccgt gacgatcgct tgttggtctc ctgttagtaa ggtatgaaca
    18781 ctctgttttc ccagacgacc tcggcgactt gtggaattag gaaaagccag attcacactg
    18841 tcttctggct tcacctcttg atattctgtt tcattttttc gaatcatgat ttttgtacca
    18901 ccttgtttcg ttgacaaggt gggggcggtt ccagtaactt cataaactcg attaccttgt
    18961 tcaaactttc ccggtaatag tcctgctaca gagattccat cccttgctgg cagtgcgatt
    19021 ttgattcctt catcacctgt taaggttggc gctacgccat ctgaaccata gacttgtcca
    19081 cccattcctc gattggaagg attgatattt accaaatttc tgatgttttg atcagatgtt
    19141 gaagacggtc caatgatagg aaatacttgt tcggtacatg cgtctcgaga atgtccgata
    19201 aggaatatcc gctcccggct ttggggaatg taggcctttg agttaagcac ctaccactcc
    19261 acgtcatacc ccaatccatc cagcgttcgg aggatcgtct cgaacgtagc ccctccttcg
    19321 tgattgagca atcctcggac gttctcaagg aataaaagct taggtcggag aagagtggtg
    19381 aaccgagcga tttcaaagaa taaagtacct cgagtatcgg aaaatctttg ccgctttccc
    19441 gcaattgaaa aagcttggca cggaaatccg ccgcaaagga tgtccactgg tccgagggat
    19501 tgaataaact catctgatac acttgtgatg tcatgcattt ccacctcctt acttgtatca
    19561 tgaattgctt tataactacg tctagcaaat ttatcaattt cacaaaaacc gatacaatga
    19621 tggctcgcct gttccatacc taaacgaaag ccaccaatac cggcgaataa atctaaaaat
    19681 ctcatacatt acctcattca ttgcaaagaa aaaagccaag aacattttgc tcttggctca
    19741 tacattatta atttgtccta ctatttttta tttaatccaa ctaatttccg ttgtttttca
    19801 ttggcctgat cggctacata tttaatcacc gttttgaact cttttgcagt agcattttcc
    19861 gataaatccg caatgctctt attgtatttc tttacaattt tcgttacttg ttctttctca
    19921 gtcatgtaat tgaacctcca aatctttcct actataccct agtggtaaaa ataattctag
    19981 attatccgta gcataatgaa cttcggaaat attattcgta taatctttta ttatgatatg
    20041 aagcaataca tctggatgtt tactctttac ttttgcaatg ttctctcttg atttgaaaaa
    20101 agcgttaata aatgcctttt tcggcacatg acgtccctct gcaatttcac gttcttgtgt
    20161 aaatttccaa gcgattttag ggtcttgata aacatagaaa atcgtcactc gaaaatgttt
    20221 tttcaacgcc cgcgagatat ttttttcagc acttaatatt gcaaaagttg catccagaat
    20281 aaaagaatag ccatgatcta agacatatct aaatgcctgc tctaccaacc aagaagatgc
    20341 tttttgaaag agactagagt tttttccatt ataatcagga aactgggtgc gaaagtaatc
    20401 cgcatcaata atcacatggt tatcataatt ctcagctaat ccaatggcaa cctctgattt
    20461 tccagcccca ggactccctg ccataaaaac tgcgtctcgt ccaacttcgg gggatttccc
    20521 tttgataata taagaaataa acttatcctt atttgctttt gcatactcta aataagaatc
    20581 attactttcc atattaatta tcccctttct tcctactata taaaagtatt atacgttact
    20641 tgaagtataa agtcagtatt gaagatcact caatcattca tcttttctaa tttactaatt
    20701 gaatctaaga aatcataccc ttttggtaca agtggcgtat aaaactcact aatcacactg
    20761 ccacctgtat ccacataacc tcgacctttg atgtgcttca taaagaagtt tttttcgacc
    20821 tccccaaaca tcatggaata tccgagttca ctcatacgac ccaaagccac gcgaaagttg
    20881 aattgatcgc ggattccgtc tcctaaatat ttcgcatccg gcctttggca agccaaaatc
    20941 agaaagaaac cagattgacg acccaacatc acgatttgtt tgagcttatt caaaatcacc
    21001 gcactttctt tcgtcgttaa catctccata taagccacgt attcatcaaa gattaaaaag
    21061 ttgggtggaa gtccaagata ggcataattc tctcctgttt tgtagttaga catttctttc
    21121 attgccttgc tacgagccat catacgttca tagaaatctt ccacacaagc agaaatttct
    21181 tccttttgtg aataaacatg aggcatcacc gtacctaaat cagctaaatc tgcatttttg
    21241 ggatcgagga taaacaattc tgcatctgac ttcaatagtg cttcgataat ggtgaggagg
    21301 aaataggtct tccctccacc cgtgccacca gcgattaaca tatggggtaa agaatcataa
    21361 gcccatactt gatttttcat caatcgcaga gtcccatttt ctgccactac ttcgtctatc
    21421 ccaattcgat tggcgatcat atcatacagt aaagtatact ccacatagga gtctttgagt
    21481 tccttttcta ctaactcaca atacagtccg ctttctaact tcttttccaa ttttaaaagc
    21541 tgatcttggt atttcccaag tgaaatttga acgcgaatag acagtaatcc atctttcatt
    21601 cgataacaga ttttggggaa gtaactgata gtctctctgg ttcggctact attcaaatct
    21661 ttgaaaaagc cttcactttg agtttgtttc acttcatacc aatggttctc tagaatcatc
    21721 cgagctagct tttgtcgatg ccacaagctt cgccaatgat caagaaaaaa gtgaatataa
    21781 agcaatatca tagctcctga aaaagcgaga cttccaagca gacatactaa atcttttata
    21841 gaccaaacga tttcaatagt agaaagtgat agcttagaaa tatcaaattg tctaaactct
    21901 tgaaaataaa agacgctcat ccaaccgatg aacagagcga gtagccagcc aagaacaaag
    21961 tgataaacca agtgctgatc tcctgcgcgt atgcgatgtc ctttgtacat tttgatggtt
    22021 tccacctcca atcgaaaaag cccaatagga tttcattagg caatttcaaa aagttatgct
    22081 agtcatttac tgctacgagg aggggagttg tttcgtttca accaattgat ttaaaaagcc
    22141 ttcaatagca tccggcatct ccacaccaat ttcttccaaa atggattctg cctgccgaca
    22201 taagttttca tttacttcga caactataaa gaaagactct tcttctccta accagtctag
    22261 tacatcagag tcttcttcac tgtcgctatt ttctccaagt aaaccttgat gtataatcag
    22321 aaacattttt tcatcagagg ttaatgcttt aacgagttgt tcctcagtca gctcgccttt
    22381 tccatattga tcgagacaat ctaaaatatg ttcaacatta atttccataa tcttcaccta
    22441 tttctttctt tcattttggt tcgtaggatt tccttgattc ggctgccgct tcaagactaa
    22501 atcatccgcc ttaatgaacc aattaacggt agttccttct cgaaaaacat aattggcaac
    22561 ggtatccacg attggattga ccaactctac ctctgcatca taagcaaact cgcgtaaggg
    22621 tacattagct gggatactaa cttgaatcat tcgcccttgt gcagaggatt ttaaattata
    22681 ggttcgttct tttacctcat taccaattgt tccatcctca ttttgcttcc gtacttctcg
    22741 gcgtaatgcg gaaaaacgca acttcccaaa tgttaaacct gaatctacga caataccttc
    22801 tgttaatctc atcttctttt cctcctatgc tttcactaaa tcatcaacat acaacacgta
    22861 atcagtaaac ccacgattct caatctcata gcctttggca accaaatggg gattcacggc
    22921 ttttagcggt tggtcttgat caaaatcctt ttcaccggct tccgcagcta ctaccacctc
    22981 aatatcatcg gcccgttgct tactggaata caaatgataa ctgcgaccaa tcaccgtagt
    23041 attccgtcca tatccttctg ttataatttc tccttcaccg gcataactta actttccaaa
    23101 ggtttcagcc atatctggca cgacaaattt caattccatc ttcttttcct cttttcattg
    23161 ttagttttgg atacaaaaat agcgcgacta cttttttagg aggcggtcgc gcacgttggt
    23221 tcttaggatt tacttgtatc atctcatcac cttagttccg gcgattccag aaataatagc
    23281 ccgccgtcgc aaaaatcaaa gtaaaaccaa tgaacagtaa aacattggaa tttttctccc
    23341 cggtttgagg gaaggtcttc actgtgggac tagattcttg agagccactt ggaacagttg
    23401 gtgtttccgg ttgtttcggc gtacttagta tggttggaac ttcttttggt gttaaggttt
    23461 gagatttttc tttcaaatct tcattgtgtt ttccattgat attcccatct ttatcgtaat
    23521 tgatttccgc aaaagtaaac ttggttcctt ctggatattt tccggtatct acttttttcg
    23581 caagcacagt tttggtgaat tctttatcat ttacttcata atcaatcttg ccagatttcc
    23641 aaatttcttt gtttgtacca tccggtaata aagcatacag aattgtttcg aaagcttctt
    23701 ttgagccatc cagtacatca tgagtaatcg acacatcatc aaacatatcc accacatcac
    23761 catgagtaaa ggtctgagaa ccgttttcta ggtgggcctt tgtttggata gaaacatggt
    23821 gttccactgt acaactgaca gtttgggctt ggttattcaa gctaacatcc ttcgctactg
    23881 gcttatcgcc tgcttcaaaa gcttccttat tttcatacac atagttgaat aagacaatag
    23941 ttttatttaa aacttgttcg gcggttaatt tatgctgaat tttccaagtt cctgttttct
    24001 ctttgttcgc caacgttgcg cttgtttctg caatcaccac tggtttggct ttttcatctt
    24061 tttcttgggc agcttttgtc gcatccacat caatggcttg cgctaccaca taccagtctt
    24121 ctttgatttc atgtagattg taactcaatt tatcaactag ctcggtagaa tccagagaag
    24181 ttaattcttt gtttccgtct ttccaagtcg caagagaagt taaactatct tcttcctcag
    24241 gcaaatcata gagcattaaa cggttcaaac taacagaaaa tgctttgttc gtcagcttct
    24301 cgtaaggaac ggtcaccgtt ttaattggtt gtttttggtc ttgttcggta atggtaaaga
    24361 catactcact cttcacaaag tcttctttgt tttccttaaa agtagaacgg atttctaatg
    24421 gcgtaatctt ttgaaaacct tctggggctt ctacttcttc aagtaaataa tccccataag
    24481 gaagattttc aaacttgcca taaccatcaa accctaattg ctcgttataa gctgtggtcg
    24541 cttcatcttc ggcacctgtg atttcattgg tcccttccaa tggcgacact ttaaaggtca
    24601 gatcattgaa gccagtttcg gtagtgcccg cagctgatcc agcaaattta aagaaatcaa
    24661 agccaaagcg aatcacttgt tcttttgccg taacatttcg ggtaatcacg gcattttttt
    24721 cgctatcatc cacctttttg atggatacag gatactttgt ttcatcaagg ctatatcctt
    24781 cgggcgcttt tgtttcttgg caataatact cgttgatagc taaatgttta acggcaactt
    24841 ggttcttttc gtctaaagtc aaagtgaccg tttcatcaga acctttcgtt cccttcgcta
    24901 actctggttt aaaagcctca ctccatttaa cagcttttcc atccttagca gtaaacaagg
    24961 tgtattcagc accctcaaag actgccttac cttgtgcttt atccccagta tctttgtctt
    25021 ctttcgtcaa agtggtttcg ccagtgactt cttgattttg cccttttacg ttactggtaa
    25081 caggggctac ggtttgattg gcatacttca attcgacttt tacaggtttg aacgtattca
    25141 cgaacccatg actagcttta gtttcggtca cataataggt tcccaattcc aacgcattag
    25201 caatctcttt tggtgtttcc gcatgaccgt tttcatccgt ggtcatttct tgaacgattt
    25261 caccggttgg actgtctttg cgaatggcaa acgtattacc agctaaggaa taattgtcat
    25321 tccaaagatc actccctgtt tcaactccgc ttttatccag aatgatttgc cctttttccc
    25381 gtgcgttttt tgaagtcacg taaatggttt cgcctgcttt aatggtggta gtcatggggg
    25441 tggtatcaat cgtataaggc gccggtactg atttttcggt aatggttact tttgtaccat
    25501 gaggaatctc atccaatgta gcaatgcctt ctttgtcagt agtcacgtct tttgcaggta
    25561 aagtttttcc aaagtctaaa tggaaaaccg ttcctggtac aacatcaccc gattctttat
    25621 cgactttttt gatctttaaa gaaccctccg tttccacatc aattttgatt gtgtaggtat
    25681 taggtttatc aatcgccccg gccattaaag tctgttggcc agccttttta taggcgacag
    25741 gtgttccggt accagcagat ttcttaagcg tcagcacacc actttctttt gaattggcat
    25801 ttggggtgat tatcaactga ttaccattca cacgataatc aatgtttgcg gcattttcca
    25861 caacctcatc gaactctgac aaattcagat tattcgtatc cgtcacagta gtcgattgcc
    25921 ctaatactgt tttagccgta ctattatgga aggttggttt cttctgataa tcgtcaatgg
    25981 cttgatttat ttttgcctca atggctgcga tatttacggc actaccatct gatcgtttta
    26041 ttgagtgaag ggtataccca ttaacttctt gccagatcat cttctgtgcc acaatatgag
    26101 tatctgcatc tgtacctact tttttccata aaacggaaac taatttggct ttatcggaca
    26161 tatctggtaa cgggtttttc tcatagccag gagtgaactc attgtaaatc gggactcctg
    26221 gttcaataca gaaaatatct tgtttaaagc cgttgtacac tgcataatgc ggaacagtga
    26281 cttctgacat tggacgacca tcactaaaag ttccttcaac agcatattga tgtgctaaat
    26341 ctagttctac ggttaccgtt tgtggatggg taatttcatc agcaaaagta atcgtaggag
    26401 caattgctcc aacaattgct tgaacagcca aactcaaaat agttaataca cataatttct
    26461 ttttttgttt tttatacatg aaatgttctc ctttcaaatt aagcacaaaa aaataagatg
    26521 ctcgcttgaa catctttgtt tataaagtct ttttaaaatt tttattgact gaatagaatg
    26581 gatttgagaa tgaatgagcc taaaaattta atctaaaata cgaaatatac tgcctttaaa
    26641 ttagaacaca ttttagaaca caaacatttc aaatttaatc aaatataata aaatatattt
    26701 acgggaaaac ctttataaat caaggttttt caaacttatt caatgatacg aagaaaaaac
    26761 caggtcttaa gaaagcccgt aaagcttcac agttctcaaa acgttaattg cgattatcgt
    26821 tcgagttcac gagttacgag aatcgtactg ggcgaagcaa tgctgagcgc agttacctta
    26881 atcctaactt ttctggcact ttccacaatt tggaaagtgc cttttttgtg tttttagcta
    26941 tgttaatagg taggattctt ggtctataat atcattcgat ctataaacaa acgggaaaaa
    27001 gataaagtat catcaaacaa tattatttat ccgcttctga ggttgtgaca gaagtggtca
    27061 gcttcaagaa ataagaagga atttacaaaa attgcttctc atgtttgata aatattgtga
    27121 cttgtgaccg aggagttggc aggagaacac cgtttattcg gaagaaggga ttgtgaaaag
    27181 tcttttgtca taacctctta tttggtagct aattgtaaat cccaaacggc atgtattatt
    27241 tttcataagt ccgtttttaa ggattaatgt tttcttttgc tttttatatg gtctggcttc
    27301 agcgagaagt tccaacgcat atacaaggac gacttatgtc aacgataaca acgattacca
    27361 tggtggccag tccacttgga ttagcactgt ttggtgcgct ctttgattgg aagacaacgc
    27421 aaccacgtct gcgagacagt ctaatttttc tgggagggag tcttgctata gttatcctta
    27481 taaccggtct ggtaaaatta ctcaagcttg atctgaaaga agcaaaaatt ttcagtgaag
    27541 aggaatacag agaaacgact ttggtcgaaa agaaagaatc aggaaactga tacaagcgta
    27601 taacaaactg cctgataata ggaaagacac gtattgtccg attgtttttt ttgacattca
    27661 attggaaaat ttacactttc tttatacatt tctggctgct ttctttatac ccgtcaacta
    27721 tacttcactc atcaataaga aagaaggata agtatggatt ttggaaatga aacggtcttg
    27781 aaagcaactg gtataaccaa aaaatacggt gctgccaaag cattagataa agtatcaatc
    27841 gaaatcaaac gtggaatgat ctatggattg attggggaaa atggagcggg taagtctacc
    27901 tttatgagaa ctattatggg tttgatctcg attgatgaag gttcgatcga attatttgga
    27961 acgacggatt tgcaggcagc aagacgacgt atgggtcaat cgattgaaac ccctgctctt
    28021 tatccagaat taactgcaag agataaccta cgtatccaag cagcgaacgg cggtgtgtcg
    28081 gatagagaaa tcgaagacct gctgaaaatg atgcgattgg aaaatacagg gaaaaagaaa
    28141 gcaaagaatt tctctttagg tatgcgccag cgtttagcta ttgcaaatgc actgatcacg
    28201 aatccagaat ttttgatttt ggatgaaccg acgaatggga tggatccagc agggatggca
    28261 gaaatgcgag aaatcatcca acgtttggta aaagaacggg gcatcaccgt cttgctttct
    28321 agccacttgc tagatgaatt gtcacagatc gccactcact atggtatttt gcatgaagga
    28381 cacttgatca aagaattatc gaaagaagaa ttggcacaag aatctcgtca atttataaaa
    28441 attgacactt cggcgacgga acaagcagta acggttctcg actctcttgg ttatagggat
    28501 tattttgtac aatctagccg tgttatccag ctttttgaag gaattgacca agtagcagcc
    28561 atcaatcaag cattggtaga agcaaaagtt cctgtggatg gtattcacct agttgggcaa
    28621 aaactggaag attatttctt acaattaact ggaggcaatc cacatgttta atctattaac
    28681 tgctgaaaaa ataaaagtat ggaagagccg taaaatgtgg gtctcactag gactgatggt
    28741 cgtacttccg cttttttatt cttggaacga atggtatatg catacaaaat acggcaatga
    28801 attaaaccaa gcaacggata cagtcatcaa cggtgcaaca gggattttaa tggtagaaaa
    28861 aatggctgca tggattcttc tggctttcgc agcatttgcg tgcttttata ttggggaaga
    28921 atttcaaaac ggtacgattc gcaatacgtt atctttagga cggaatcgaa tgacttatta
    28981 tgtgtcgaaa cttctcgtta cgcttttgat cacaacactt ggcgtggttt tgatcacggg
    29041 actagccatg cttgcttaca cacttgcttt cggatttggt gaagttgagg gaatcaaaaa
    29101 ctatggaaat tatgttctaa aagtatttcc tgtattgctt ctgctgacat tagggacatt
    29161 atctgttcca gtggctttga cattcatcac tagaagtacc agtgtcagtt tgcttctttc
    29221 ttttctttat atcatgggaa cggccttcat tcccggtgtt ttcgcaaaaa tcaaaggctt
    29281 ggaatttcta acggagtggt ttacggaaac gtggctcatg tatacggact ttgcgcaaca
    29341 agcaacttat tcgcaagcac cgaaaatgat tctggtcagt ctagtaacaa tcgtggtatc
    29401 atcagctcta ggaatgttta ttttccaaaa atctgacatt aagtaggtgg tcctgctgga
    29461 aactattttg attatcgaag acgatgaagc tgttcattcc ttactggagg aggttcttga
    29521 acagagatac aaacttcttg atgcttattc aggtacggaa ggaaaattat tgttaagcac
    29581 atatcctgtc gatttgatcc ttctagatct catgcttcca gggctttcag gtgaagcatt
    29641 gttagcagaa atccgacaga catcgaatgt tccgatcatc gttctttcag caaaaagcga
    29701 tcaaagagat aaagtttcct tgttggcagc aggagcggat gactatgtga cgaaaccatt
    29761 tgatatcgaa gaacttttgt tacgaatcgg catccaactt cgtcatcaga cttctgtcca
    29821 agtaaaagat ctgtcgcaac gcatctatta caaggaaatc ttactggaca aagagtcgag
    29881 ggatgtaaat gtgaatggtg tggctgtcca tttgacagga agagaattcg atctgctgaa
    29941 gcttttttta gaacatccga aaaaagtatt ttcgcgagca aatctttatg aaacagtttg
    30001 gcaagaacca tattttgata gtgagaaaac agtgaatatg catatttcca atcttcgagg
    30061 gaagctagca aaggcacatg ctgtgtatat tcatacggtg tggggagtgg ggtttagatt
    30121 tgattaaatg gctgttgctt ctcgttgtta tctttatggg aagttttatt ttcttcctgc
    30181 aaagggaaat caagagatta actaagaaaa tcgccactct ttctgaacga caagaatatg
    30241 gtggtcgtct ttcagtcgaa tttcgagaaa agaatctaat ggacctagta gatgcactga
    30301 atacatggtt ggatgaaaat gaacaaaaag gacaagcatt tcaagaaatg gaagaaaaca
    30361 tcagactatc gatcgtcggc ctttctcatg atctcagaac tcctttgact gctattaacg
    30421 gttatgtaca attactggaa caggtagagg atacggagaa acgggagaag tatttaacga
    30481 tcatcaaaca atctgttgca agattactca gtatgacgga tcagttttat gatcttgctc
    30541 gaatagagac gaagcaaaaa gaaatggatc tccatacgct ttctttttct aaagaggtgg
    30601 aagaagtatt tttctctttt tatgaacaat tttctgagaa aaatatcgag gtagcgttcg
    30661 ctgaacactc gataaataca actgtattag cagatccgct catgttgctg cgagtgctgc
    30721 aaaatatcat acagaattta ttgcgttatg cagatggaca agcaaaggtg acattttcaa
    30781 aagaggatcc attcattcga ttaacagtgg ccaatcctat caaaccagga acgcaaacct
    30841 ctgttgaaaa agtcttccag cgtttttata ccgaaaatac cagccgcaca aatacggaat
    30901 ctagcggaat cgggctttac ctgtcaaaaa aattggtgga aggaatggga ggagagatga
    30961 ccgctagatt agacgggggc atcttctcca tctccgtcaa gttgcggagg gtttaattat
    31021 acaagattag tgagaactag tagaaaaatg tattatctta tatatattta atagagaaaa
    31081 aagcctgtga tataaatatt gcaggctttt ttattgattt gaaataatca agaatattag
    31141 aaacttctac attaatcaag aactttcttc tgtatgaaaa tagagataat aattgtacac
    31201 ttaagctgta ttcaggaggt gaagataatg ggcatcgaat ttagcgaacg gcaaatgaaa
    31261 atactatact ttttgattga aaatgagcac acaacttaca aagaattatc agaacaatta
    31321 tatgtatcta cgaaaacaat tagcaaagat attttaattt tgaaagaaat aatccaaaaa
    31381 atgccagatg tacaaatgtt ggttcaacct aaaaaaggcg tttcgcttat tggaaaaaaa
    31441 gaaacacttc gaaatttatt atccagtcat aaaatcaata tcgcagactc tccggaggac
    31501 cgtctaactt atgtgtttgc taaattattg aatactaatg attctctaaa gattcaaaat
    31561 cttgctgacg aattatacgt gagcaggtcg acggtagaaa gcacaatgaa agatgttaga
    31621 aaaagactaa acaagcatgg gatgtcctta gtttcagatc gaaatggcgt gcgcatcaaa
    31681 gctgatgaaa aaagtaaaag aaggttgatg tcagaagttg tgaactatta ttggggtggc
    31741 tttacagcat ctaacagtaa aacagatctc ttagacttga atatcaattt cattggtgaa
    31801 gcgaataatt tcttaaataa ggacatattg aaaaaagtag ccgaattgct taatcgatac
    31861 atgaataaga cacaattgaa atttacagat tatgaatttc aaactttagc tatccacctt
    31921 gctattgcga tagaaaggat tgaaacaagc aatttcttat cacgtagtaa taattctgaa
    31981 gaagaaatct caaagaatac acttatttta atcaaaatga ttgaaaaagc attttcaatt
    32041 attgtgccaa tagatgaaca agcttatgtt gacattcaca ttacttcgat tgaaaaaaat
    32101 gctatgaatc acgacttaga tcaaacgaag ctttcctatt tagatatggg tgttgagcta
    32161 caagagttag taagaattaa attaaatgaa ttttatcctg atgaagaatt gatcaataat
    32221 ctagtattac atctcaatgc agcagttaaa agactcaaat tgggagtaaa tatctataac
    32281 ccttatacgg acaaaatcaa atattctttt aaaaggtcat ttatgataag tgtagattta
    32341 ttagaggaaa tcgaagagag attctgtata cattttaatg aagatgaaat cgcttacgta
    32401 acactccata ttcaatcact tttggatcgt tacaaacctg acaaaacgaa agtgatatta
    32461 gtttgtagta gcggatatgg aacatcaaaa ttattagagc aaagaataaa taacggtttt
    32521 gcagatatgg ttgagataga agatgtacta agcatcaatg aactacaaga tagaaaagtc
    32581 actgatgaac ttgttataag cacgattccg attgaaaata caaattttcc tgttattgtt
    32641 gttagtcctt tgatgttagg ggcagatgtc agaaaagtaa aacagtatct acagttaaat
    32701 gaacaaacag aagatgcttt tctaaattta ttagataaac gattttgctt ttttacccac
    32761 caaataaagg ataatcaagt gaacgtttta aatataatta ccaaaaaatt ggtaaaagaa
    32821 aattacgcga aagaagggat actagaggga gcaatcaaaa gagaaaaact atcttcaaca
    32881 gcgatgaatg attttgctat gccgcatatc gaagtagatt ttataaaaaa gccagtaatc
    32941 tgtgtctacg ttaatccaga aggaattgat tgggatggga cacttgttca ttctgttttc
    33001 ttttttgcac ttaatgaaaa agtaaaacct gaattgaata aaatttatga aacattcaat
    33061 gaaatcgctt cgaatcatga gttattagca aatttaaaaa cgagttcgtc gtttgaaaaa
    33121 ttagtttata tattgaaaga aggcttataa atgatcagtg aaaaaatcaa tataaataat
    33181 atagtgatag atttaaatgc atctgataaa aaaagtgtag tcgaaaaatt atcgaaattg
    33241 ttagagacta atgggtttgt gtctgaccac aaacaatttc aaaaagacgt atttgacaga
    33301 gaaaaccata tgaccacagg aattgggaaa aatatcgcca ttcctcatgg caaaagccta
    33361 gcagtaattg aatcaacagt ggctgtagcc aagttaaagc ggtctgttga atggggcgca
    33421 ttagatgatc aaccagttga tctcgtcttt ttgttagcaa taaaagacac tgataaaggt
    33481 actaatcatt tacgtatttt agcagaatta tctggaagat tgatggatga cgagtttgtt
    33541 aaaagaataa aagattcgag aaataaaaaa gaattggtaa aagctctatc attttaaaag
    33601 gaggaatttc atatgacaaa aatagtagca gtaacagcat gtatcgcagg gattgcccat
    33661 acttatatgg caaaagctaa tttagaaaag tttgcgaaga agaaagggtt agatattaaa
    33721 gtagaaacac agggggctat ggggatagaa aacaaacttt caatttcgga tgtttccgag
    33781 gcagatgttg tcatatttgc agtagataca aatgtttcag aacgaggtcg ctttgacggt
    33841 aaaccgattt taacagtagg gacctcagag gtagtaaaag atggtgaaga gacgattgat
    33901 gaagcaattg cattaattaa caaataaaaa atatatcgtg ggggagttac taatggaatt
    33961 caagaaacaa ggaagagaaa ttctaaatca tttccaaagt ggggtttcct atatgatccc
    34021 gcttgtcgta gcagcagggt tactaacttc tattgcagtc atttttggtg gcacaggtgt
    34081 ttgggaccag acggatacat tctggggagt cctgcgaatg attggtcaga cgggattaca
    34141 gtttatcgta ccgatgattt cagcctatat cgcatattca attgctgacc gtcccggtct
    34201 agcaccagct tttatcactg ggatgatgtg tcaaaatctt ggtatgggat ttattggagg
    34261 aatggttgct ggattagctt gtggctattt agctaatgca ttgaaaaaag taaaagtacc
    34321 gatgcaagtt attagtctaa aatcaattat gctgattcct tttgttacta cattagttgt
    34381 tggaatcata ttatggtatg tactagctac gcctattcaa gcaatgacaa caggactgac
    34441 taattggcta gaaggcatgt caggagcaaa tgctgctttg atgtcggcta ttttaggagg
    34501 aatgatggcg tttgatatgg gaggacctat caataaaatc gcatatgctt ttggggttgc
    34561 ttctttttca agcggaagct ttggacctag tacagccatg ttattaggaa ttgctattcc
    34621 gccatttgga atgtttttag caacaatttt aaacaaaaaa ctttatgatg aatcggaacg
    34681 agataatgca aagacagcta tgattatggg ccttgtgggg attactgaag ggacgatccc
    34741 gtttgccgtg aaagatcctt tacgagtaat accaagcatc gtggtgggca ctgcagtagc
    34801 atgtggtcta aatggatttt ttggtgttac tcaagaaaca atgctttcaa cctttatggc
    34861 aattcctttt gttacgaata tacctttata tttagtatca attgttattg ggggagtggt
    34921 tacagcgtta atggtcaatg cactaaaatc catcaagtat aaaaaagaac aacaagtaaa
    34981 ttcagtagaa gaaggaaagt aggagaatct gatatggtaa atgcacatat aattcaacac
    35041 acgcactggg acagagaatg gtattttaca aatgaagatg ccatcgtatt gtctgatcag
    35101 gtctttactg aagttctaga tgaattggaa aggaacccta aagtaaactt ttgtttagat
    35161 ggacaaactt caatcgtcga tgagtatttg gagatcaatc cgcaaaagct taaggttatc
    35221 caacgactga tgaaaacagg acaactattc gtgggaccgt ggtatgcaca aacagatgcc
    35281 ttattagtgg atgctgaatc aattttaaga aacttaatga ttggaatcag tgatacgatc
    35341 cttaaatatg gagatccaat gatggtaggg tatttgcctg acacgtttgg gttcaatgcg
    35401 aatttgccta cacttttgca tcaagtagaa atcgataatt tcatgtgttg gcgaggatta
    35461 aactttgaaa cgatggttcc atctccttat tttatctgga aaggattggg agataaattt
    35521 gtttatgcca tgaattttcc atttggttat atgactggaa tgatgacctt agacgcaatt
    35581 aaagatattc ctgaatttgc aaaagaaaag ttggatgaag cagttgaaat gcttcatatg
    35641 caaggaaaaa acaaagatat tttagtacct tcgggtgtag atcaaaagtc aatgatttta
    35701 aattttgatc aagtcgtaga gaagttgaat aaatacagta aattccacaa tattatttca
    35761 gattatcctt cttatgtaga agttatcaga aacaaaaaag aattacctga atataaaggt
    35821 gaacttcgtc agccggttta ctctcgtgtt catcgttcta ttggctcagt aagaacaaaa
    35881 atgaaaatag aaaattttaa attggaacaa aaaattctaa aacgagttga gccgctcatg
    35941 ctgattgcag aaagaaatgg tatccatatc agtaaaggcc tacttcgtcg gttatggaaa
    36001 aagattttgc aaaaccaggc tcatgatagt attggtggct gtgtttcaga taatgttgcg
    36061 gaagatattt tccatcgaat caaagaagct aatgagatag ctgacggaat cgaaaacctt
    36121 attgtaaaac ggatggctga tgcattaaag ctaaaatcga atgaagtcct tgttttcaac
    36181 acaacgactg agaattatag tggatcgaaa atcgtgcatg tagtagctga ttcaaagaaa
    36241 atacacttca aaggtagtcc ctataatatc attgaaaaag aagtttattt tccagaacgg
    36301 aaaaatgctt atcggatgat tgctacaggt ttcgaatatt ttgatgaacc agcttattat
    36361 gaacttgaca taaaaataaa tgtaaatgtg ccagcactag gatacactgt tgttgaattt
    36421 gagaaaaata atgaaaaatt agaaacagca atcgagatag acaaaactga gataagtaac
    36481 aataaatata aactttcttt taaagatggg caattgaatt tgatagttgg agatagacaa
    36541 tatcttgatt tcgttcattt gatcgattca gcaaatgatg gcgatacgta tgattattca
    36601 ccattagaag gagatacaga actatctcta aaattagaaa cagctaaagt atataaagat
    36661 tctttacaag aaacattagt tgtttacgga aaagctcaac ttcctaaaga tttaaaagat
    36721 cgtttatctg aaaaacccga aatggaagaa atatcttatg aaatatcgtt cagtctggga
    36781 gaaagtcaaa tcgttgaagg cacactcaaa attcataata ctgtttactc gcatcgtatg
    36841 cgcttagcaa ttaatgtttt agataaatct gaaaaatcaa ttgcacaaat tcaaaacgga
    36901 tttattgaaa atacccctga tgtcattcct ccgaattggt cagaaactat ggtagagaaa
    36961 cctgttaatt tagaaatatt cgataagagt gtctctgtgg cagaaaatga tcattatttt
    37021 acgttttatg cagatggttt aaaagagtat gaacgtatag aaaatcaatt aatcataacc
    37081 ttattttcaa caacagggga acttggaaaa cctaatcttg cttggcgacc tgggcgagct
    37141 tcaggagata cgacaaatga agggcatgtc atgatggaaa caccgctagc tcaagagata
    37201 ggtgagtata aagtcacttt tggatttaat gtagaagaag gaagatttaa tgagttcaaa
    37261 gtggcaaaac aggcagaaaa acggttagaa caatctattt cttatcaaaa gcagaaactg
    37321 aatgtattta ttcacaggtt agataacaag atttggccat tacaaaatga acttgatgtt
    37381 cctcgtaaat tttcattgat tgaattacct gaagacttaa ttgtatcatc gatttatcct
    37441 tcttattttc atgaacaagg atttgttctt cgcttagcta atccaacaga acaagaaaaa
    37501 atagtacctg aggctatcct ttcccttggg acagttgtca atgcactgga gaataaacaa
    37561 gaattaacaa caattcctcc ctatgattat ttgtcaattt tgataaatga gagataaata
    37621 tgtaatatac aagaaaaaaa caccttttat tttgaaaggt gtttttttac cctcttttca
    37681 aatagcgcaa cagtgctata ctaaagctaa caaataaagg aacggagagg gatatgggtc
    37741 aaagagaatt tatcgtttta attattattg cagctgttat tttattagtt gctttagaca
    37801 ttttttcacg tttgaaatta aaggaaacag ttcgtagcaa atgggaaaaa attccttatc
    37861 aaccgagatt tgataaagaa gaaagcttga aggaagcatg gctcacggag aagaagttcc
    37921 gttcatggga tagcgaaatc gatgatctta cttggtatga cctggatatg ttcgaagtat
    37981 tcgaaggaat caacagtacc tattctagtg taggttcaga agcgttgtat caacgtttac
    38041 gcagctttga ttttggtgaa gatcagcagt tagaaaaact gattgctttt tatcaggaaa
    38101 atcctcaact gagagaaaaa atccaatatc aattcgctcg tttaggaaaa aaagatcata
    38161 attttgcaaa acaatatcta gcggatggaa aaagtaaaaa gattggacgc gtaggtttat
    38221 ttatttttct tggcttgctt ccgctggtgg gtttgggttt gcttctttta ggacaagtac
    38281 taggaatcta tttggcaatc ggtgggttag tattcaatac ggtctattat ctagtgaaga
    38341 aacaaacact agaaacagag ctgaatagca tgagttattt ggtttcaacg atttcagccg
    38401 ccaatcagct agcaaaactg ccgacaccac ttcaagaaga gctgaaacag caaactaaac
    38461 cattaagaga catccctaaa ttaggttttt catttcgtat gaagagtggg agtgaagcag
    38521 agttgatttt tgaatatgtc aacatcatgt tcatgctgcc atttatctcc tatcatttcg
    38581 ttctcactaa attagaaaag cattcaactg aagccatcaa gctgtgggaa ttgctgggag
    38641 aattagaagt agccgctgca atattgaatt accgtactta tatgcccatc acttgccgac
    38701 cagagtttac tgagggcggt gtgacagcga aagatatcta tcatccattg ttaaaagaag
    38761 cggtagtgaa tccggtcaac tggcagaaga atacattagt cacgggttcc aatgcatcag
    38821 gaaaatctac gtatgtcaaa agtatagcca tcagctgtat tctagcacag acgatccaaa
    38881 cagcgattgc agaacagttt accctgcaac ctggacatgt gttgacttcc atggcaatcg
    38941 aagatgattt atttgaaggc gacagttatt ttgtttcaga aatcaaatcg atcaaacgtt
    39001 tgttggacca agtggcttcc aaagaacgct gctactgctt cgtagatgag attttaaaag
    39061 ggacaaatac gatcgaacgg attgcttctt ccgcttcagt cgttcggtgg cttgcggagt
    39121 atccatcttt agcatttgtc gccacacatg atatcgaatt gacagagatt ttaaagaatt
    39181 attgtgagaa tgtccacttt gaagaacaag taacagaagg aaaaggaatc agttttgatt
    39241 tcaaattaaa agaaggacct tcaaggaccc gaaatgcgat tgccttgctg aaagttttgg
    39301 attacccaga gactttagta gaaaccgcaa aaacggaagc cgcgttattt gatgaaaagc
    39361 ggcagtggca tgtattggga taggcgaagt gatcgaaagt atttattgga tcaaaaatct
    39421 tggcttttgc ttccgccgtt tatacgtgtt tagagcgtga gacaaaagtg ttctttactt
    39481 ttgtctcacg ctccttttgt tgcacatatg aatagtaatc ctttatcttc gatttgtttt
    39541 ttctgaaaag gttgtctgac ttcgtccgat tgatgtcttt cattaggaga ttttcctgag
    39601 cccagcaacc tgatttttcg ctggaaccaa gttgtttttt catctgctgt attccatgat
    39661 acaataaaga atcatgtcta taaggagata ggaaataaat ggcaaatgcg cttgaaataa
    39721 ataatttgaa aaaggtttat ggaagtgggg tcgaagcatt gcgaggaatc gatctttccg
    39781 tgaaagaagg agatttctat gcgctattag gaccaaatgg agctgggaag tctacaacga
    39841 tcggaatcat tacttcttta gtcaataaaa catctggaaa agtgaatgtc ttcggttatg
    39901 atctggatac ggatctagtc cgagcaaagc aacagattgg attagtcccg caagagttca
    39961 atttcaatcc ctttgaaacg gtccagcaaa tcgtcgtgaa tcaagcaggc tactacggtg
    40021 tctctcgcaa agaagcgatg aaacgcagcg aaaaatattt gaagcagtcc aatctttggg
    40081 aaaagagaaa tgtccgtgca cgtatgcttt ccgggggaat gaagcgaaga ctgatgattg
    40141 ctagggcatt gatgcatgaa ccacgtttgc tgatcttgga tgaaccaaca gcaggagtcg
    40201 atattgaatt gagacgcgag atgtgggagt ttctccgaga attgaacgaa aatggtacaa
    40261 cgatcatttt gacgacgcat tatttagaag aagctgaaat gctttgtcgc aacatcggga
    40321 tcatccaatc aggtgaactg atcgagaata ctagtatgaa agagcttcta tccaaactgc
    40381 aatttgaaac atttatcttt gatttagcgc catatgagac caaacctgtg attgaaggat
    40441 acgcttgtac atttgaagat gaacagacaa tcgctgtcga agttgaacga aatcaagggg
    40501 tgaacggaat cttcgatcag ctgacaagac aaggaatcaa agttctttct atgaggaata
    40561 agtcgaatcg tttagaagaa ctgtttttga aaatcacaga agaaaataat catacggagg
    40621 agaaaaatgt ttaatcttta ttttactgca ttaaaaagtt tagctatcaa ggaaacgaac
    40681 cgctatttgc gtatctgggt ccaaacactg gttccgcctg tgatcacgac atccttgtac
    40741 tttgttattt tcggaaaaat gatcggtggt cggatcggtg acatgggtgg tttttcatat
    40801 atggaattca tcgtgcctgg tttgattatg atgtctgcta tcactagttc gtatgccaac
    40861 gtttcttcgt ccttcttctc tcaaaaattc cagaaaaaca tcgaagaaat cttggtagcg
    40921 cctgttccta cgcatgtgat catttggggc tttgtgattg gcggagtggg tcgcagtatt
    40981 ctcgtcggct ctctcgtaac gatcatttcc ttgttctttg taccacttca tgtgtattca
    41041 tggttcatgg tcatcattac attattgatg acggcaatct tgttttcgtt agcaggactg
    41101 ctcaacggga tttttgctca gtcttatgac gatgtatcga tcgtgcctac atttgtcttg
    41161 cagccgttaa cttatctagg tggcgttttc tatgccatct ctatgttgcc gccattttgg
    41221 caagctgttt cgaaagtcaa tccgatcgtt tatatgatct tcggtttccg ttacggattc
    41281 cttggaacaa tcgatgtgcc agtagcaaca tcgatgatcg tacttgtgct attcatcgtt
    41341 gttttgtacg ccgttacttg gtatttgatc aataaaggaa gaggattgag aagttagaaa
    41401 agaggttgtg aaatcagcgt tttgacctga gtattagaga aacaaacggg aaaatagttc
    41461 ctcatatttt gtcgtttgtt gagctaatac cgagggtcag ctgattgaac accgtttagt
    41521 ggagaggttg tgaaaaagct gttctgcatc aagtaataag aacagccaaa caaaaatagc
    41581 tcctcctatt tttcgtttgg ttggacttat taccgcagat gcaagctttt gaacaccatt
    41641 tattccggaa aaggaattgt gataagattt ttgtcacagt ctttttttgc tcaatcagaa
    41701 acatctttta agctgttcac tttgcttctt tttttgtttc ctagtataat gacaatgttt
    41761 ttgagaaaga ttgcgacttc actgcttgat agaatggaga gtaacaaata aatgactact
    41821 aatttttgga aagagctgcc aaagcctttt tttgttttag caccaatgga agatgtaaca
    41881 gatgttgtat ttcgccatgt cgtaaagcat gcagcagcac cggatgtctt ctttactgag
    41941 tttacaaact cagacagttt ctgtcatcca gatggaaaag acagtgtacg aggaagattg
    42001 acctttacag aagatgaaca gccgatggtt gcccatattt ggggagacaa gccagaattt
    42061 tttagagaaa tgagcattgc tatggcagaa atgggcttcc aaggaatcga tatcaacatg
    42121 ggctgcccag tccctaatgt agcagaacgt ggaaaaggca gtggcttgat ccttcgccca
    42181 gaagtagcgg cacaattgat tgaagcggct aaagctggcg gtttgcctgt cagcgtcaag
    42241 acgcgtatcg gctatacgga aatgtctgag atggaagaat ggatcagtca cctacttcat
    42301 caagatatag ccaatctttc tgtccatttg cgtacgcgta aagagatgag taaagtagat
    42361 gcacattggg aagtcatccc taaaatcatt gctttacgtg accaaatcgc tccgcaaaca
    42421 ctgatcacga tcaacggaga tatcccagat cgccagacag gagaagaatt ggccgaaaaa
    42481 tatggcgtag atggcatcat gattggccgg gggattttca agaatcctta tgcatttgaa
    42541 aaagaaccga gggaacatac agaaaaagaa ctgatcggac tacttcgtct gcaactagat
    42601 ctgcaagatc attattcaga aatcatcccg cgttccatca caggactaca tcgtttcttt
    42661 aaaatctata tcaaaggatt tccaggagca aatgacttgc gagttcagtt gatgaatacg
    42721 aaatctactg atgaagtacg agagatctta gacacctttg aaaaagaaca tccaacgctt
    42781 ttcagtgaac aatgatttct tgcttgaaca ccgtgaatta gaggtcgtga aaaagctgtc
    42841 ctgcatcaag taataagaac agccaaacaa aaatagctcc tcatattttg tcgttttttg
    42901 agcgaatacc gagggtcagt tgattgaaca ccgtttaaaa gaggttgtga caaaagtctt
    42961 gtcgcaacct cttttttaac caaaaaagtg caaatagttt actttaagta agagaataat
    43021 tatgatgaat aaaagtggaa caaatagcat actatatata ttgaaatgaa aactgtatgg
    43081 atgctccaac ttgatgaagc taaggaggaa ttcgtatgtc agaaaaggaa ttaatgaagg
    43141 tagtcggttt ttatgaaggg ttgtcgatcg atgatccaaa aagctttatt gatgaaaaag
    43201 aacagatacc tgtacccagt tcacgggatc ttctggtaaa agtcaacgca gtatctgtta
    43261 atcctgtcga tacgaaactt cgccaagata acgggataag aaatgcttta cgtatcttag
    43321 ggtttgatgg agtcgggaaa gttgttgctg taggtgatga agtgcaaaaa ttttctgtcg
    43381 gcgatcgcgt tttttacgca ggtacaacga caagagcagg aagcaatcaa gaataccaac
    43441 tggttgacga acgtattgtc gctcttgcac caaaaaatct ttcagatgaa gaagccgcag
    43501 ccttgccgtt gacctcattg accgcttatg aattgttatt tgaaaaattt ggtctgactc
    43561 cagaagaaaa tgccaaccgc gggaaaaaaa tcttagtgat caacggagct gggggcgtcg
    43621 gttcgatttt gaatcaacta gcgcattggg ctggcctaga agtctacgcg actgcaagtc
    43681 cgaagaattt tgaatggctg aaaaaaacag gtgtagacta tccaatcgat tatcatcacg
    43741 acttaagtaa aagttttcgt gaattaggaa tcgatcaagt agagtatgtc gctgtattat
    43801 tcgatattac ccgctacttt gatcaaatca aacaccttgt ccgaccattt ggacatgtgg
    43861 gaacgatcgt agggatcaaa gagccgttag atataggtgt ttggaaaaac caatctatca
    43921 gcttcgactg ggagtatatg ttcgctaaaa cagactataa ctatgaaatc gaaacgcaag
    43981 gagaagcttt agcgcacatt gctgctttag caaatgaagg gattatccgc agtacattga
    44041 gcaaggtcta ttcagatggg atgaatgcga agaacttgaa acaagcaacc aaagacgtag
    44101 aaacaggaca tatattagga aaagtcgttg ttagtggtcc gttcaacgga taagcaagaa
    44161 tacgccgatt tttttcttat ttgactttga taaaaacaag agcgcatgac agtaatttgc
    44221 cataagctct tgttttttta tttataataa gcgaatcaac gaaagggaaa gaattcctac
    44281 caagacgatc accaataagc tttttgaaag aatagcagct aaaagtgtag gaactgaagc
    44341 caaagcattt tcgagattga tctgcggcag atgtccgata ttttgggtaa atagactgct
    44401 gaaccataaa gcagacataa tgacgatcgg gacaaaactt aaatactcaa gcaacggttg
    44461 cggcaaatcg aattttttca gtaagacaaa tggtaggatc ctagaaagcc aagttgctaa
    44521 agtacaacct aaaatagtca gcaatgtaaa ctcaaaagaa ggcatgtttg atccacaccc
    44581 ccaatccgca cccaatcagt gttacaacta aaatcactaa gttacttggg ataaaaatca
    44641 gtccgacata taccagaatc aaagtgagac caatcaggat caactgtaat cgtttagagg
    44701 tgtttctatc agaaatgact tgaagataaa gtaacccgat aaacatagcg acgatggcga
    44761 aatccaaacc aaactttttc ggatcagtaa taaaattccc taaaagtgcg ccaacaagag
    44821 aagaacccac ccacgtcaaa taagaaatcc aattagctgt attcatccaa cgaaagttta
    44881 acttttgtcc agtatagttc aatttattca ttcccagtgc gaaactttca tctgtcagca
    44941 atgtgccaat cagcagattt tgaagcagcc gatttttctt gaaataagga gcaatcgtca
    45001 tactcatcag aatcattcgg gaattgacta gaaaggtcga gagaacgatg gagagcagtg
    45061 gactatggct agcaagcatg ctgaccgtga taaattgggc agaaccagca tagatcagca
    45121 gggacatcaa cgtgacgacc agcggatgaa acccggcagc tttcccaacg ataccaaatg
    45181 caagtccaat accgatataa ccaaaaacgg taggtaacgt gtctttgatc gcagttttta
    45241 tatctaactt ttcatccaat aacaaaactc ctttagcacg acataaattt tcataattgt
    45301 cgtcaaactt gtgaatattc taaattttta atgaagactt gtcaacttca attttaaaca
    45361 aataaaaaag actggacgta gtttaaaaaa atcttctcaa gttagatttc cagacctaac
    45421 ttctgaggat cactacattt gtcacagtct cttatactga caagtataaa tgaagtatga
    45481 agaaaaagta tttgttattg gtatttctca ttattcttgt tttctaatgt gttcacttca
    45541 agtggaattt tagaatatta ttcaactaac aagtctacat cgtatctaaa tccatttgta
    45601 tcacttaatt gcttgtaaaa ttcgccactt tttttaattg ttcttttttg agtagtaagg
    45661 tcgagtgaaa ccagcccata tcggttttta taggcgttga tccatgacca gcaatcaata
    45721 aatgtccaaa gatgataccc cttaatgtta catccttcat ttattgcctt ccataagtaa
    45781 gcaagatgat ctttgatata ctcaatccga taatcatcaa ttatttgccc tgtatcatcc
    45841 atgaaacgtt cttcatccgc aacgcccatt ccgttctccg agataaatgc ttcgatgttt
    45901 ccataatggt tcttgatatc catgcacaaa tcataaattc ctttttcgta aatttcccat
    45961 ccccggtatt cgttcattcg tcgtccaggc atgatatgtg gttcgaaaaa ccactctggt
    46021 aaaaatggac tagatgtatc caccatagaa gaacgagatt tgacgcgacg tggctcgtaa
    46081 tagtttagtc caagaatttg agcggtattt tcgtagatac atacaagatc ttctttcgta
    46141 taatcttcag gaagctgatt atattcttgt aataacagga taagcttttt agaaaaattc
    46201 cctttaacca taggatctaa aaagctccgc gtataaaata aatcagcgat ttctgctgct
    46261 ttcacatcag caggattttg actacgagga aaagtaggac tcaaatttag aataacgcct
    46321 attttactag gaagagctag ttctttaaat acttgtattg cttttgcgtg tgcaagtata
    46381 gtgaagaatg ctgcttgcgc tccgcgttta aagtcaatga ggtttggata atgaaaatct
    46441 tcaagataag tacctaaaat aggcccaagt ggttcattga aggtaaacca atgacaaact
    46501 aagtcaccaa attctttaaa acaagtttca gcgtaatgaa cataagcatc aactacttca
    46561 cgtgattccc atcctccttt atcttgaaga gtagcaggca tgtcaaaatg atataagttg
    46621 gcaaatactt tgattccgtt attattcatt tcggaaaata attttcgata gaaagaaatg
    46681 gctttgggat ttacttttcc ggtaccgcca tttggaaaca ttcgtgccca agaaatagaa
    46741 acgcgaaagg agttatgccc agtttttttc attagttcta tatcttcttt atattgatgg
    46801 atgtgatcgg ttgtaataga ggaatcgatt cgttgataaa accggtaagg ttgttcttta
    46861 aaccagcgat cccagatagt ctctgctttt cctgtttctc cttttccttc tgcttgctca
    46921 gctgaccaag cggaacccca ccaaaaatca tctggaaaag ttagcttttt catagttgtc
    46981 ataatttttt tacctcctgt ttccacttaa tgataacacc gcttacaact taaagtaaat
    47041 agttttgttt aaatatatat atctttttta ttgtgtgtat aattttgatg tggattttat
    47101 cggtttattt atgttaaagt atagatgaag atatgttttt gattggaggg tcaccattga
    47161 aaaaaattgc gaagtatatg gaaatttact tagatataaa gaaaaaaatt gtaaatggag
    47221 agtataaaat tggtgagaaa cttcctacag gagatgaatt ggcagttatt tatcatacaa
    47281 gcaagttaac agtaaagaaa ggcgtagact tacttgtttc cgaaggagtt ttgcgaagta
    47341 gaagtgggta tgggactgaa gtcgtcagaa caccaattga taactcacga gttcttggtc
    47401 caaacgaagg tttgtttagc gtggttggtg aagaacacgt agattcagaa atccatactt
    47461 tctcaattga acttccatcg aaaaaagtag ctgataaatt aagtatcagt gtaaaagact
    47521 atgtatataa tattgtacga agccgtttta ttgattgtca accatactcg attgagcaaa
    47581 cattcatgcc attagcagtc ataccaggct tggaaccaaa acatttaaag aaatctgtat
    47641 atgcttatat cacaaatgaa ctgggtctgg aaataaagac ttcgcacata cgtatcaaag
    47701 gagatactgc gaatgaattt gacgcaaaaa tattaaaaat agatccaggt gaatttatga
    47761 ttgagataga caaagttgtt gctctatcgt ctggtaaacc tttcgagtat tcggtatcga
    47821 gacatttata tcaagacttt gtttttgaag ctgtttttgt tgaaaaataa gctcgggaaa
    47881 attatttttt cagagcttat ttttttatac taaaaaagat atatatattt aattaaaagt
    47941 atttactttt tttgataagt gatgtattat ttgggtggaa aaagtaatcg attacaataa
    48001 gcataaagga gaaaaaatat gggcaaaagt agcaggaagg acattgtatt tgaaaaattt
    48061 ggtatagttg cgactaagtt ggggaatcag atccatttaa gaactttgcg agatgcgttt
    48121 gctacattta tgccatttat gatgttagca ggatttgtga cacttattaa ttcagtgatc
    48181 ttagaaccaa caggattcat gggggaaatt gtcgatacta gtacattgac cacgttacaa
    48241 caaattggta cttcaattgc caatggtaca ttaagcataa ctacattatt agttgtagca
    48301 gcagtatctt atcatatgtg tgcaagcaga aattatacaa atcatattgc agcggtatta
    48361 gttgctattt cgacatttgt tgtattaaca cctatgcaga tgatgtttac tcctgaaaat
    48421 gcggataaag ctgttgaagt gacgggtgtg atacctatat cctatacagg agcttcagga
    48481 atgtttgtag gtatatttgt tggattagcg gctacagaat tatttattaa actttctaat
    48541 aataagaaac tacagattca gctatccgga aatattcctc ccgctgtttt aaagtctttt
    48601 aatgtattaa tcccaatcat tattacgatt acctcgtttg ctttattgtc atttataatc
    48661 attcaattgt ttaatatgga tataaataag ctaattacca cgatagttac agagccatta
    48721 agtaaaatta caactggttt accaggattt ttgttaatta catctattgc taatctattt
    48781 tttggatttg gtatccatca agcagtaata tcaggttcat tgttagatcc gtttttgatt
    48841 cagaatatgc aagaaaatat ggcagcttat gcaaacaaag agcaaattcc acatattatc
    48901 aatatggcct ttaaagatac ctttgcggta atgggtggat cgggaaatac gattgcttta
    48961 ttgattgcaa tatttatctt cagtcgtaga aaagattata aggactttgc taaattatcg
    49021 gtaactcccg ctatattcaa tataagcgaa cctattattt ttggattgcc tattgtattt
    49081 aatatcagtc tgattattcc ctttgtgctt gcaccgattt tttctttgac agtagcatat
    49141 tttgcaactg ctgtgggatt aattaatcat gtagtagtgc aaattccttg gactacgcca
    49201 ccagttatat cgggattttt agcaacagcc ggagactggc gagcagctgt tttacaaata
    49261 ttaattatag ccgcaagtgt gtttatatat ttaccgtttt tacgaattga cgaacacgtg
    49321 acaactaaat taagtgaaaa tgaaacataa tttactatga gataacaaaa attaacacaa
    49381 gaagtcgttg gaatataaat agccagtaat ttcaataagg atcatatttt ttgataagta
    49441 cgaacatgta gaaaaacaaa cattttttgg taaggataat cattattttt cacaagacaa
    49501 acctatctgt gtatatacgt ctatttagta tatgcaaaga taggtttttg gtggtgaaaa
    49561 ataaagactg ttaatattat taatcgttat tttaatattt atctttaaag attagagtgg
    49621 ttcgacacac ctgaaagttg ttgtcagaat tttatccaat atacagatgc agtggaacct
    49681 ctcttacaaa atgtagaaaa gtatcataaa atctatcaac aatgccggaa aatctaccca
    49741 gcaacgaagg aaatttgtca tgacttataa aacaaaacgc gtacctaata gtcaaatgac
    49801 taatattggg tacgcgttaa gtcttcttta cttcatcctt tgcatgattc gtgtttttaa
    49861 agcaaggtct gctgtatttc ttgtggattt aggatggttg ttatcgttta cttgctattt
    49921 ttcattgaaa tcgtctttct ttaccgtttc tttagttttt ttatctattt ttttcttttg
    49981 gttagaaaat tctttttccc gaaagtcttc tttcaggatt tttatggagt agcgttttga
    50041 ataagaggcg tcagtatgat cgacttggca gacgaaccaa gagaacattt gccttttttc
    50101 tgcatgaggt gcttctgcta ataattcttt ccataaataa taacagtcct ctatcagaag
    50161 aagggttcgt tcatcctcag gctcccaata gaatgtttcg agagtagcaa tcacttcatt
    50221 gatcaattcg aatgcgggaa gataggcatt ttgctgaatg agcgattcaa ctgcttcttg
    50281 gatgaacttt cgcatctcca tgatgaactt cgctgttttc ctgtttttgt ttttcttgat
    50341 aatatgatcg tatttatgga tgatagttgt taatttatct gaataattta ttgcatgaaa
    50401 tggcatctta actttgaagc gttcgataaa ttgcggatgt tctataagaa tctctgttaa
    50461 aagctcccgt acaagagagg aactagcgga ggaaacaagg gacgagagtt cggaaagtga
    50521 gtagttggat ttttttattg gacgttcttc gtaagcgaat aagacagcag ccatgtgttt
    50581 gcattttttt ccttctatgg catatagaca atcacaagtc atgttggtct tttcatccca
    50641 agtgatcact accttataat cttccgtgcc agaaactgtt gcggacaagt acttgcttgt
    50701 atagttcaaa tgacggacaa aaccgtgcag atgatagctg taaccgcgtt ccaaaacgtt
    50761 ttcaggaaaa tactcaagcc aattattcat ctagttctcc tttcatgaaa cacttattta
    50821 tatttagtat aaagaaacgt ttaagaaaaa agtagctgat tttacatatt tgataatcac
    50881 ttttgctcga attgtcaaaa attcattctc cttttctttt cttagagaat gtgagaaaaa
    50941 gtaaaagaat agcgtgtttt aaagatggaa ctttttttgt tttttattta tctgacaaaa
    51001 atgaagtgat tttgttagaa ataaataatt gttccaattg ataaaagagg tatgatgtat
    51061 cgcaaagaaa aatccaagca gcgttctgat cagcagtttg aacaacaatt tgctttgtac
    51121 agtaggtagt aagcggatta actaagggaa taaaagatat ttttaccgct ttgtttactt
    51181 agaatgtgtc ttccccaaaa tttatttttc taaaggtaaa acatagatag gcagataata
    51241 ccaattatcc gttacgaaac gctgcttgag aattttcttt agatccatac aacattttct
    51301 acatctgact aaattggttc ctgattcatc aagtaattga ctttctttga taatatgata
    51361 aaaaggcaag caaggaggaa gataaatatg cttaaagatc tgaaccaatt gatcgattac
    51421 atcgaagaac atttgacaga aggactttcc tataaaataa tagccgaaaa tacaggaatc
    51481 tctgaatatc aattaaaacg catctttacg tttattgcag ggatgtcgct aatggactat
    51541 atcaaaaacc gtagattggc tttggctaat cgcgatctcc tagatggatc aagagtgaca
    51601 gaaacggcgt ttagatatgg atatgaaaca gtcgaaggtt tttcaagagc ttttcgtgat
    51661 tggtcaggat atctgccttc agaaatcagc agatacggca tacaaaaatc ttttccacgc
    51721 ttgacatttt ttattgatgt cagaggagga aaatcgatgg aatttcgtat cgaaaagaaa
    51781 gaacagtttt atgtggcagg tgtggcgaaa aaagttcctg tcaaatttga aggagtcaat
    51841 caagaaatac aaaagttagc gcagacgatc acggagactc aaagggaaga gatgcataag
    51901 ttaggagatt tatatccaaa tcaagtaatc aacgtttctt atggatttga tggagcccgc
    51961 ttggaagaaa agggcagttt gatgcacatg attggctttg ctacgacaaa agaaaatccg
    52021 tacgctgatt tagaaatgct tgctatagaa gaacacactt gggccatttt tcctaataaa
    52081 gggccatttc cccaaacttt acaagatact tgggggaaaa tctatgcaga atggcttcct
    52141 tcttcagatt atgagttagt cgaagctccc gagatctctt ttacaaaatg gggagaggat
    52201 ttcagtaatg tttacagtga gatttggatt gcagtgaaag caaaaaaata gttaaaaaat
    52261 aaaaatatcc ctagaaaaaa gagcgtaatc gtgtcatttc cctttaattt tatgtaagat
    52321 gaacttaata taatagaaag ggtgggagaa atggcaaagt ataggatcga agaaaaacca
    52381 gattttacgg tgacgggtat tggtgtggaa ttgaagagta gcaatacgga ttttgtcggt
    52441 ttggctgcag agaaacaagc atttgagcaa aaagttatcg gtaatggaac aatagatgca
    52501 ttgaaacaag ctgctgaaaa tcattatttc tttacagtca acgaacccta taacgggaaa
    52561 atgatgtact atgtcggtgt tcaaacgagt caggatgttc caaacactac ccgaatcatc
    52621 ccattcccaa aaggaacgta tcttgttgta atcgctttag cagaatcgcc tgaagaatta
    52681 agaaatgatt tgacgaatac ggcctttggg catgttttaa gagaagtaga agattacgcc
    52741 tatgttgggg gtccaaatgc tgtagtcgaa atgggagtta ccaacgggaa atatatggga
    52801 gaaatctgga taccggtagt caataaacca tgataatata gaggtcgtga aagtagcgct
    52861 ttgacctgag cattagagag gaaaaatggg aaatagcttt ccatattttg catttttcag
    52921 aactaatgcc gagggtcagc tacttgaaca ccgtttatca agacgtcatg acttcagcgc
    52981 tttgacctga gcattagaga ggaaaaaggg gcatgacaag gcttttgtcc cccggagaat
    53041 aaaagaattt atgtagacga ttccgatcag aaaaatgcag gcacagtaga gagaattata
    53101 aaaatctctc tacgaggcct gtgttttctg gtcttttttc tttttccaaa acggctattt
    53161 tatgtatcag actatcaagt gtgatcttag ccatttcttc ttctgccttt ttctgcagcc
    53221 gagcgtagct ttcttctaaa gcttcttgga tattcgcacc aacgacgcaa tttggatttg
    53281 tttttggatc gacttggata agctgtgtat taccttcgat gctgcgatag acatctagta
    53341 aagaaatctg atctggactt ttggcaaggg aaggctgcgg ttttccggtt tttgtaaaga
    53401 tcaaaccgga ttttttgagc tgactcatga ttttacgtac atttgctgca tttgtttcga
    53461 tactactggc aatcatctca ctagaaagtg atcgggtatc ttgaaacatt tcaatatagg
    53521 caagtacatg gattgcatca ctaaattgca gagaatactt catcagttgt catcctttca
    53581 ttttttttgc ttgacttatc ttaccacact ctttatgata aaggtgtaat ttataaatgt
    53641 acagctgtaa atcttaacaa ataaaaagaa tagggagtgg caaatatgca ttatttagta
    53701 acaggagcaa ctggaggttt tggtggttat gcgctggaat tcttaaaaaa gatggttcca
    53761 caaaatgaaa tctatgtact agtacgtagt gaagaaaaag gagaagcact gaaagaagct
    53821 ggatttaata tacgtattgg tgattatgat gatttagact caatgaaaga agcgttgaaa
    53881 ggaatagatc gattgctttt tgtctctggc gttccaggaa atcgtcaagc cgaacataag
    53941 aatgtagtga aggcagcaca agcagctggg gtatcttata tcgcttatac aagttttgca
    54001 gatgccgatc actcgatcag tgtattagcg ccagatcacc aattcactga aaaaatgatt
    54061 aaggattcag gtatttccca tacattttta cggaataatt ggtatgtaga gaatgaatta
    54121 cctatcattg ggcaggcttt aaaaactggt caattcgttt atgctgcagg aaatggaaaa
    54181 acaggttggg cattgaaacg tgaatatgca gaagctgcag caaaagtact agtagaaagt
    54241 gactctccag aaatcttaga attatccggc aatctaactg actatgaaga attagccaag
    54301 gcattagagc gtgcgacagg taaaaagtta gaaattattg aagcaagtga tgcagcgttt
    54361 gtcgaaaatc taaaagaagc cggattccct caagaagcag ctgacatgtt tttagcgatc
    54421 caacatgata ttaaaaagga tcagttagct atttcctctg atgatttaga aaaagtattg
    54481 ggaaaaccat taacttctct tgaagcagca ttaaaagaaa tattggatca ggaataatgt
    54541 ataaagaggt tgtgaaaaag ccgattagtt ttgagtatga agaaaaattt tgtgaaaatg
    54601 gctttccaca tttttgcata aatttgactt cataccgata aactggtttt tgaacaccgt
    54661 ttatcagagg ttgtaaaaaa gctgttctgc atcaagtaat aagaagagtc aaataaaaaa
    54721 tagctcttca tatgttacgt ttggctggac ttattaccgc ggagttggca ggagaacgcc
    54781 gtttttattc ggaaaaagga gctgagacaa gatttttgtc tcagctcctg tttgtttatc
    54841 gataaaaaag tctggggaag acgatggctc aggctttgtt ggaaacagag ccgattttat
    54901 catttgcttt tacatcacgt gcgggttcga tttctaagct ttggactttg tcgccatttg
    54961 tgaatacaac gaccatatcc gtgtttttgc ctgcagattg aatagcggca agatcaacta
    55021 aagcaatcaa ttgacctctt gcgacttttt gaccttcttt tacatataaa gtgaatggtt
    55081 cgccttttaa gtcaacagtg ttgatgccca tatgaagcaa gacttcgata ccagcatctg
    55141 tttggattcc gacagcatgt ttcgttggga aaatgtttgt gatcgtacca gcgattggag
    55201 agaagatttc tccattttct ggtaatacag catagccgtc acccatcatt ttttccgcga
    55261 atacatcatc tgaaacttca gtgattggaa tcagtttccc atttgcgata gcgtaaatat
    55321 cttgttcgat cgttccaccg gcaaatgttt ctgttttaac cacggtgttt gtttgtgcag
    55381 gtcgtgttgc agcaccacca tgtagttttg ccatctcatc tgcaacgaat tgtacttctg
    55441 tacctacgat gacttgcgca ttttttccgt cgatgatctt cataccaggt acgccagtgg
    55501 ctttgatttt attttgatcg atcaggcctg tatctttgac ttgcaaacga aggcgtgtag
    55561 tacagttatc gatcgaagtt acattgtcgg ctccgcctaa tgcagcgtag attttgcttg
    55621 cttgtgccgc atatttattg tctgaattag tcgttgcagt atcgtcatct gaaagatctg
    55681 cttcctcgcg gccaggagtc atcagatgga attttttgat agcaaagtta aagccaaagt
    55741 agtaaatcac tgctgttact aatccttgga cgatcaacat caatggctga ttcgcaatcg
    55801 gatttttcaa agataggaag taatcgacaa atccggcact aaaggcaaat cctgctgtcc
    55861 aatggaagaa tgcactcact gctaaagaaa tcccagtgaa aattgcatgc aagacatata
    55921 atggccaagc aacgaacata aatgagaatt caagtggttc tgttacacca gtaaagaatg
    55981 aagcaaaagc tgctgctaac atcaaggaag ctgtttttgc tttgttttct ggacgtgcat
    56041 ttctgtaaat agcatatgct cctgctggaa gcccgaacat catgattggg aaaaatccag
    56101 cttgatacat tcctgttacg ccttttgttc cttcagaagc caaaaattta ccaatatcat
    56161 tgattcctgc tacgtcaaac cagaacacgg agttcattgc ttgatgcaat cctgtaggga
    56221 tcagtaaacg gttgaagaag ccataaagac ctgcaccacc ccagcctaaa ctcaaaatga
    56281 atttagcaaa agaaactaaa gcagagtaaa ccggaggcca tacgagcagt agcactgcac
    56341 tgatcagcaa catgacaaag gcagccaaga ttggcactag acgcttgccg ctaaagaaag
    56401 ataaagccat cggtaatttt gcttcgtgga aacgattata aagagctgcc gcaatcaatc
    56461 cggcaataat accaataaat acgttactga tctgagcaaa agatggatca actgattctg
    56521 gtttgatatt caataaagta gctactgaat cagtttttaa aacatttact ggaacttcaa
    56581 atgcgaccaa acctgccaag gcagcagcac ctgatttgtc cactgacatc cctagtgcta
    56641 acccaacggc aaacagcaaa ggtaattgac ctaaaactgc gttaccgcca gcttgtaaaa
    56701 aatgagcgac aatgtcgtta ctataagatg cccaccagtt tccgatccct actagtaaag
    56761 cagcagcagg taagaccgca actggaagtt gaagggaacg tcccattcgt tgtagatatg
    56821 ctttcaattt ttttcctcct catgattgtt aacatttgtt tgtacgattg cgcttcttaa
    56881 acgctcgata tggatggcca agtaacccag ttcttctttc gtgatcgcga gctggtattc
    56941 tttggtcagc aacacacgga tttttttaga aatcttatag ctttctgggt attttcgttt
    57001 gaccatttct acgatttccg tgtctaaaac agcatattgt tgcgcatggg ttcgttgtag
    57061 aagcagtcgt aaatgattca ccagtcttga ataattcagt gaaaatggtt ctgaatggat
    57121 atctgtttcc aattctgatt ccactaaatg gatgatgtca gatacaatcg tcacttcacg
    57181 gatactgcga tggttattgt tccgaccgct tcgtccgctg tgaatatgga ttgcgatata
    57241 tcctgcttcg tcataagtat atggaatccc aagctctttt gtcagatact ctactgccca
    57301 ttgagcaata ctgaattcat cactgtaaag cacttcgatt tctcttaaaa gtttgttgcg
    57361 gatgacgata ccgttattaa tgttctccgc agaaaaagca atgtggtcgg tcaaagcgat
    57421 caatacatgt tcattcaact tttccatcaa aacagtttcc gcatgatcaa tgatcgtttc
    57481 tgctgcaaat aaaaagcgtt catcaatctg attaagtaaa ttttgcaact tgatttgacc
    57541 ttctggttcc atgacaaata tccgttcgac atcacgatca aaaatcaaat catttctttt
    57601 tctgtcaaat ccaatccctt ttccaatagc tactttttcc tgtccaccgt catctaccaa
    57661 tactgcattt tgattaaaga tctttttgat tttcatgcgc gcaccctttt tgaagatttg
    57721 gttttcacgt ttgctagacc agttgattgc ggccaaacaa aaatagacac gaaaaacctc
    57781 ctagggaaac catgttcctc agagattttt catgcctagt atgatctagt cacatgaaac
    57841 gaattattgt taacgtaatc aattttatat agacagttat gttttgtcaa ttcttttttt
    57901 ataattatag taatttactg gaattatcag aaataaaagg aaggaacaaa ttaagaaact
    57961 tatgttattt aatcattgga tcttttgctt aacttattcg ttgttaaaaa gctgaaagcg
    58021 aaggaaaaaa actctactta actgtatttc tccgttagga catctaaaaa tttacgagca
    58081 gcaggagaaa gaggctggtc ttttttccaa atgagattca gtgaagcttg taatggcgga
    58141 tcgagtggga tgaatacttt ttccgtccca taagtattga tgatcccatc tagagctaat
    58201 gcaccaaaag tcccgttatc gaccatcaaa gaagcattgt acaataagtt atatgttccg
    58261 acgatcgata actgatctat gctgaaacct agccattcag ctagttgact gtccactgac
    58321 gtttgccggg aaatgatcaa aggaacagtt ttcagttcct gcggatggat gactgtttgc
    58381 tttgctaaag gatgattccg atgtatcaaa agtccccaac gatcttgggc tggcagtcga
    58441 aatgactggt atttttgttt gtttgtcggt tcgattacga gtccaaaatc aagtaatccg
    58501 ctatccagtt tgtctatgat atcatctgca ttcccgctat ataaatgaaa ctggatatca
    58561 ggatgcgcct tgatcaacga atgaaggcta tcagaaatga aagagattgc tttagtttcg
    58621 cctccgccaa tgtagatttc tccagagatg attgcgtcat ttgttaaatt gttcgccgtt
    58681 ttttctacca acgacaagat ttcttttcct tttgaataaa gatatcgacc agaatccgtc
    58741 aactgtatct ctcgggagcc tcgagtgaaa agtgtcacac caagttcttc ttctaaatct
    58801 ttcagttgtt ttgagagagt aggttgcgtg atatgcagag attttgctgc tcggctgata
    58861 tttttttctc tagctactgc taaaaaatac gtcagtatac gaatatccat tttttccctc
    58921 ctgtttttaa aaaagcatac cactaagtat agttaaaagg aatagttaag tatgttttat
    58981 aagtattaga catcataagc cgtccgttat aaagtaagga taaaaggagg gagcaaaatg
    59041 acaagcctca tcgaaaaaca aatcgtcaat aaagagattc gagtaacgga tccgctcttt
    59101 gaagtgatcc atcagatcca agcggaaaac gaagagcctt tagcagcgct gaatactggg
    59161 taccatgaac cagcagatat ccgcaaacgg ctagaaaata tcattagtga caaagtagat
    59221 gatacggtga ctgttctgct gcctttttat acagatttcg gcaaacatat tagtataggg
    59281 aaaaatgtct ttatcaatcg tcaagtgatg ttcgttgact taggtggcat ctgtttagaa
    59341 gattcagtat tgattggtcc tcgtgtcaac ctcatcacgg tgaaccatct aaccgatcca
    59401 aaagaacgaa gaggcttgtc agtaaagcca atccacatca agaaaaatgc ttggatcgga
    59461 gcaggagcaa ctattctgcc aggcgtaacg attggcgaga atgccattgt tgcagcgaat
    59521 gcaactgtta cgaaagatgt cccagacaat accatcgttg caggaatccc tgccaaaatc
    59581 gtcaaacctg tcgaacggat tatttgaaca gtaagatgaa ataagagata gtgagtataa
    59641 gcaagagatt tttcaaaaca ggaggagaat agaatgaatg atttatcaga aaaagtgatt
    59701 gtcatcatgg gcgcttcaag cggaatcggt gaagcaacaa cgaagctatt agcagaaaaa
    59761 ggggctaaac tagttatagc tgcacgccga gaagatcgcc taaaagcaat aaaagaatct
    59821 ttaccagaag ctgagctata tattcaaaca gcagatgtaa gagattttgc acaagtccaa
    59881 gcagttatcg atttagcgat ggaaaagttc ggtcgaatcg atgtattgta caataatgca
    59941 ggaatcatgc caaccgctcc attagttgaa ggtcaccgtg atgaatggca aaatatgctt
    60001 gatatcaaca tcatgggtgt gttaaatgga atttccgccg tacttccaat catggaaaaa
    60061 caaaaatctg ggcatatcat ttcaactgat tctgtggcgg gacatgttgt ttatcctgat
    60121 tccgccgttt attgcggaac aaaatttgct gtccgtgcaa tcatggaagg attacgtcaa
    60181 gagcaacggc aaaataacat caaatctacg atcatctctc caggagctgt tcaaactgaa
    60241 ttatatcaaa ccatcagcga caaaaaggct gctcttgaac ttcatgaagc gcaaaaagaa
    60301 tggggattaa catctgaaga tatagcttct gctgtagcat ttgcgataga aacgccagac
    60361 cggatgtctg tcagcaacat gattatccgt ccgacgacac aggaagtata atccataacg
    60421 gataatatcc agagagaatt acttgttaaa tagttattca aacgaccatc aatttaaggc
    60481 gtgaatggag aagaatattc ttcatttacg tctttttatg cagaaaaagc aaatttatta
    60541 aaaagaaaca aatgttattt aaatatggac attgacttgc ttttactttt ttagttacac
    60601 tgttatttag gaaaagtttc ataattcaaa ggagaaaaaa gatgaaagca aagaatttat
    60661 tatttagcgt tattctttta ggaaatctag cagttgccaa cacaacagtg ctagctgaag
    60721 aatcgacaga aactgtcgca actcagacat cgagttcttc agaaacagga attgaaaacg
    60781 atacagaaag tactttgcag tcagctactg tctcagaaaa tacaatttca accgaagaac
    60841 caacaactga aacaacagaa caaacacaaa ccgaagacag tacatcagca gaagctcaga
    60901 acaatccaga gaatgcagaa gaaatcacga accaattgac agatgggcaa acattgacac
    60961 atacccatca agcagatttg tctgaattcg aaaactatac tggctatgct ttttatgctc
    61021 gctcatctgc taattctcaa caagcgttca ttgatagtat tgcctcaact gcacaaagtt
    61081 tggcaagtgc caatgactta tatgcatcag tcatgattgc tcaagccatc gttgaaagtg
    61141 gctgggggaa cagtactttg gcttctgccc ctaattacaa tctatttggg attaaaggaa
    61201 gctataatgg ccaatctgtt acgatgccta cttcggaata tgtgaatggg caatggatta
    61261 cggtgaatgc cgcttttaga aaatatcctt cgtacaaaga atcattgcaa gataacgtaa
    61321 cggtattgaa gacgacttct tttcagccag gcgtttacta ctattccggt gcatggaaaa
    61381 gcaatacgaa ctcttacaaa gatgctacgg cttggctaac aggtcgttat gccactgcgc
    61441 ctaattatgg ttctacattg aacaatgtaa tcgaaaccta taatctgaca cagtatgata
    61501 cagcaccaac ttcaacttct ccgatgtatc gtctgtataa ccgtcataca ggagaacatt
    61561 tatatacatt gaatgccgga gaaaaaaatt atctgccaac tgtaggctgg gaatatgaag
    61621 ggattgcttg gcaagcacca aacagcggac aacctgtcta ccgtttatac aatccttaca
    61681 gtggagacca tcattacaca atggcacaat ctgagatcaa ctctttaaca aaaatcggtt
    61741 ggcgttatga aggtctaagt ttttattctg gtggttccaa gccaatttat cgactattta
    61801 atccaaatga aaaaactggt acccatcact ataccttgag tgctaaggaa agagattggc
    61861 taacaccgat gggctggaga tatgaaggaa taggatttta tggttattaa aaagaggtag
    61921 tgaaaaagct gccctgcatc aagtagtaag aacagccaaa caaaaacaga ttttcatgtt
    61981 ttttgtttgg ttgaacttat taccgcagat gcaagttttt gaacaccgtt tactcaaaaa
    62041 aggttgtgac aacatcttgt cataactttt ttattactgt ttaagcatac tgatccaatt
    62101 tctaatattc gacgggattc ttgcaaaagg cgatttcgga aatttttggc ggtgtaaaat
    62161 tatctactgc agaagagcta tcttctatag tttgataaga atcaataatc ttggttttag
    62221 cgttgtgctt tctttatcat tttttccgtt cattctacaa aaaatttctt ttctctatac
    62281 ttttctattg agagcgtttt ttttattaca cagctgattc tttgactttt ttcttttttt
    62341 attctattat tgacaagtca atcgttgacg agtcaattat tagaaaggag gaaattatgt
    62401 ctttagaaga gattagtcac cttttatatc aaatcaaatt agccgatcaa aaaatcgtcc
    62461 agatctttga agaaaatatc ggtataagtc ttactagata cgagatcatg atgagactga
    62521 aagaagaaaa tccattgcta caaagcagtt tgcaagaaaa gttgcagatt gatcaaggag
    62581 cagttactcg tcatctgaag gtattggaag caaatggcta tgtttcgcgt cagagaaatc
    62641 ctcagaacaa ccgggaagtg tatgtagcgc taacagaaaa agcccagaag aaaatcaagc
    62701 attgtgaaat ggaccataaa gatacaggtt ccgtgctatc cccggctttc agcgatgtag
    62761 aaatgaaaca atttctaaca cttttagata aattgaatac ccaactttgc aatataacaa
    62821 cagaaacgaa agaggttaat aataatggaa caaaaaaatg atttttcaac aattatgact
    62881 ggtagaagat ccgttcgcgt atatgatgaa acatacaaaa tcccacacga agaaatgctg
    62941 gaaatgatca aagaagcgac gactgcacct tcttctgtca atatgaaacc atggcgcttt
    63001 gtggtcgctg aaagcgagga agcaaaagaa atcttgcgtc ctttgatccg tttcaatacg
    63061 agacaaaatg atacttcttc tgcaatggtc atgatttttg gcgatatgca atgttacgaa
    63121 tatggcgaag aaatctacaa ccaagcttac gaatcaggga agatgccaaa agaagtcaga
    63181 gatcaacaat tagctgcgat cattccttat tacaagagtt tttcaagaga agaaatgaac
    63241 gatgtagtca aagtcgattc tagcttagcg gctatgcaat tcatgttagt tgcgcgtgat
    63301 catggctatg aaacaaatcc aattggtgga ttcgaagcag atcagttggc agaggcattt
    63361 ggtttggata aagaccgtta tgttccagtt attattttat caataggaaa agcggtagaa
    63421 gaaggttacg agtctgttcg tttagcacca gaaaagatca ctatgttcga ataaaaggtt
    63481 ttcagttact attcagtagc tattgctttt tttgaatcgt gtgtggtaac ctaataaaaa
    63541 aggaggtcgg aaaaatgact gcatcgatga aattacgttt cgaacttttg aataacaact
    63601 aaataaattc aaaagacatg tataaacaac aatttcatgg atggagtatg tccatttttt
    63661 cgaacgacag tagaataggt gaaagctatg acagatttgg tgatttagtc atagttattt
    63721 cgttcagagt aggaaaagat gtgatgcagt ttttttgcaa acagattttt ctgctctttt
    63781 ttagtatcga taattgttgt tttacatgaa ataggagtgt aaaacatatg gaaaatttag
    63841 cagtaaatat aacaaatcta caagtcagct ttggcaacca gctagaacta tctattgatt
    63901 ctcttcgtgt ctatcagcaa gatcgtatag ggatcatagg agaaaatggt gtcggtaaat
    63961 cgactttgct caaactaata gccggtgaac tttttcccga tcatgggaaa atccaaacag
    64021 agatcacctt caactacctg cctcaattaa cctatctcgc tgaagcaaag gacctaaatt
    64081 tggaattagc cagtcatttc cagttaagac tggaagaaac ttcagagcgg aaatggagcg
    64141 gaggggaaga acgaaagatc gagttgatac gtcttctttc ttcttatgaa caagggatgc
    64201 ttctagacga gccaacaacc catctagata gaaaaagtat tgatcgactg attgaagagc
    64261 ttcgttatta ttatggtacg ctggtttttg ttagtcatga tcgctatttt ctagatgagc
    64321 tagcatcgaa aatctgggaa gtaaaagacg gagaaatccg agagttttcg gggaattata
    64381 gtgcctatct cactcaaaag gaattggaga aaaagactca gctacgagaa gcagagtcga
    64441 tcatgaaaga gaaaaaacga ttggaaaaat cgatccaaga aaagaaaaaa caagcggaaa
    64501 agttggaaaa agtgtccagt aaaaagaaaa agcaacaaat cagactggat cggttgtctt
    64561 cctctaaaca aaaagacagt gtacaaaaag ccatccaaaa gaatgcgaaa acattagaga
    64621 gaagactcca aaaaatagga gaaacaacca agccgcagca gatgaaacaa atccgttttc
    64681 cagtaccaaa atctcttgaa ctccacagcc gctatcccat catgggacaa aatgtccaat
    64741 tggaaagaag tgggagaaca ttactggtaa atggtgattt tcagttttct ttaggtaaaa
    64801 aaatcgcgat tgtcggtgaa aatggttcag gtaagacaac cttattagaa catatccgca
    64861 aacaaggaga aggaatcctt ctctctccga aagtaagctt tcaagtatat cagcaaaagg
    64921 gttatcaaat gacatctgaa gaatccatca ttcgttttgt catgagacaa acagagtttt
    64981 cagaatcgct tgtccgtagt ttgctgaatc acttagggtt tgctcaggaa actttgacga
    65041 aaccgttatg tacattaagt gggggagaag cgacccgtct gacgattgct ttgcttttta
    65101 ctaagccaag taatgtgttg ctgttagatg aaccgactaa ttttattgat atggcaacga
    65161 tcgaagcttt agagaagcta atgcaaatat atccgggaac gattttgttt acttctcatg
    65221 attcttactt tgtcgagcgt acggctgatg aagtttatga aataaaaggg cagaaaataa
    65281 aaaaagtact tacgagaaat ttttaaatgt gctacgatga ttttcgtgac aaatttaaag
    65341 gagtggtaca gattgcctac aaataaaaaa gaaggaataa tttttacaac aatgatgtgt
    65401 tttttgatgg tcttggggat gagcgcgtat aatttatggc tgcatcaaga tcttcattta
    65461 tcaagtttgt tgattggtct agtgcctgga ttcgtggtgg ctttcatttt ggatgtcttc
    65521 atcatcggtg tgctggcgaa aaaaattgcc tttaaactac cagtaaatcc agaaagcaag
    65581 gtccaaatga tcttaactat ttcatgcttg atggttttag gaatggtgac atgtatgtct
    65641 ttattcggtg tgatcatgga acaaggaata cctgatcaat tattctctgc ttatatgaat
    65701 gcttggaaaa tgaatgtgat tgccgcttta ccgttgcagt tgatcatcgt tggcccgctt
    65761 tcaagaagtg tgctaaaagc aattcaaagt aatgaatgat ggaacactgt tatttgaaaa
    65821 aaggccttgg catgcttttt gtcatagtct cttgaatgca gttctagaaa aatagtgatc
    65881 cgaacgatag gttttggcct gtcgttcgga tcgctttttc ttttccaact gtattgctct
    65941 tttattgaag ataatttgct cttcaataga tatttctaga aaggttctgt ttatccctta
    66001 ctcaaaaaac tgctgcattt gttctcttac ttgctgacac cattggagtt cctgctgaca
    66061 aagcataatt ctgcgctgga tcacaacata acgtccaaat tcttgtttct tgtctagcga
    66121 gaggtcattc aatttttttt gatttttttg catgattttg ttcagctgtt gttctctagt
    66181 gaatagcagt ccagtagctg ttgttttatc gagcgtcgaa aaagcataga tttttgctag
    66241 aaattcatct ttttgcgtag gagcgggagt ttcctctttg atccattcct ctacaagagc
    66301 acgtccttca tcagtcaagt ggtagatctt tttttgagag tgctcgtctt tgtccaaaac
    66361 ggtcacataa ccctgctggt ctaacttggc aagcgttgta tagatctgac tgtgatgggc
    66421 ttcccaaaaa attgtgatca gctgcttcaa ttcataccca gaacgtggtt tggataaaag
    66481 cacgcataat aatatatagg ctaaagtatt cataaaaatc ctacctctcg tcttattttc
    66541 tttattatat cacagtaaag tttccacgtt ttacatgtgc atattgacat gtaaaataaa
    66601 tcatgtatga tgctaataga gagaaaggga tggaaatatt ggagaatcaa atagatatac
    66661 aagaacaaac aaatgaagaa acaaagggga aaagttcagc gctagttgta gcagctacct
    66721 gtatgatcgc ttttatggga gtgggtctag tagacccaat cttaaaaacg attgctgcac
    66781 agttaaacgc tacgccagct gaaacgaccc tgttatttac tagttacatg ttagtcactg
    66841 gtgtggtgat gctatttaca ggtttcttat ctagtcggat cggtttgaaa aaaacattga
    66901 tgacaggatt gtttatcatc gttgtattcg ctggtctttg cggattatct gggaatatcg
    66961 gaatgctgat tgggttacgt gccggctggg gtgtcgggaa tgctttattt gtctctactg
    67021 ctttagcaac gatcgtcagt attttagttg gtaacacaga aaaagcaatc atgatgtatg
    67081 aagcagcact tggctgcgga atggctgttg gccctctagt cggtggggtc ttgggcagtg
    67141 gttcatggcg ttatccattt tttggggtag catttttgat gttttgcggt tttatagccc
    67201 tagcggtact tttacctgaa actgagaaac caaagagaaa agtaggcctt tgggaaggtg
    67261 tcaaagcgtt aagcaatcat aaacttcgtt cagttggact aatcgcattg ttatataatt
    67321 tcggattctt tactttactt gcgtatgcgc catttttatt gattggctat tctgaattag
    67381 aagtcggttt tgtatttttt ggctggggtg tgcttctggc tattgcttcg attttcgttg
    67441 caccggctct tgagcggaag tttacgactt atcggacaat tttagcagca tttgtcttat
    67501 tcattatttg tttgctgtta ttaggagctg gaacttctat gccagtcctt gtaccgatca
    67561 gcattgtgtt agcaggattt ttccaaggaa ttatcaatac actgttgacg acgattgcta
    67621 tggaaatacc aggtctgcaa cgaaacgttg cttcgtccag ctatagtttt gtccgctttt
    67681 tcggtggagc tttagcacca tttattgcag gtgtgattgg tgaacatatt ggtgaaagct
    67741 atgcgtttta ttttgcaggt gccattgttc ttatcagtgt atgttgtatc atttatcacc
    67801 gtaattattt tgctacaaat gaagaagaat aagaaatcag aggttgtgaa aaagctgtcc
    67861 tgcatcaagt cataagaaca gccaaacaaa aatagcttct catatttttc gtttggttgg
    67921 gcttattacc gcagatgcaa gcttttgaac accgtttatc aggaggttgt aaaatcagcg
    67981 ttttgtcctg agtattagag aaacaaacgg aaaaatagtt cctcatattt tgccgttttt
    68041 tgagctaata ccgaaggaca gctgattgaa caccgtttaa cagaggttgt aaaatcagcg
    68101 ttttatccta agtattagag aaacaaaacg gaaaaatagc ttctcatatt ttaccgtttt
    68161 ttgagctaat accgagggtc agctgattga acgccgttta ccaagaggtt gtgagactgt
    68221 cgtttaactc tgaatcacaa ggaacaattt caaaaaacag tttctcatgt ttgttgaata
    68281 ttgtgactta tgattgagga gttggcagaa gaacaccatt tattcggaaa aaggggatgt
    68341 gacacgattt ttgtcacatt cccttttttt ctcaaaataa tgaaactgga aaattttcag
    68401 taggtaagct aaaattcatt taagaaaaag aatgggggga taatagatgc tggaagaata
    68461 ttttcaggaa gtcgggttga cactcgttga attacccatt gaattttcag atgatcggtt
    68521 gatttatcgt ggctatatca aagaattgcc aataatcaat gcggaaggtg aaactagaaa
    68581 agcgatgcag caaaaaatag ctggtatgta tcaggtgtat cgggaacagc tactggctga
    68641 atcagcggaa gaagaaaaag aaaaaacgat gaacttgtcc atggatgagc tgcttcgtta
    68701 ttatgatggc gaaacatttg acggattttc gcttcttaac gaagaggaaa cagatttacc
    68761 aggcagctgg taggcgaacg ccgtttatat ggaaaaagag gctgtgacaa aatctttgtc
    68821 acagcctcta aaaagtccgg cagttttttg ccgatcttca aggcactcta cttatcgtac
    68881 ggatattttt tagcggaaag tccgattcaa gtggttttta ttcagctgat atttataaga
    68941 taagtgaaaa atcccagcaa tcagtagcgt aatcgtataa gcgattgaat gatcgaaaaa
    69001 agaatagtaa ttgattgtcg ctaataagca taatgcaacc atgtaactaa tgtgcaagcg
    69061 tgtaaccagc cctttttgcc cgattttgtc aaaatactgc gaccattttt cccgttgact
    69121 ccctatgctt cgttttttga attgaaagaa aagggataaa ataccgactc ccagaaaagc
    69181 agcgatattc agagaaataa ggtaaataaa aaaaatcatt aaaatttcaa acatattgac
    69241 acctttctag ttggatagaa cctttacaga aaaaaggcaa aaggcgggaa taatgtttca
    69301 cacgaaacat tattcgcgga tttgtccatc gccataaatg atatattttg tggaagttaa
    69361 ttcatttaac cccattggac cacgggcatg caacttttga gtagagatac caatctctgc
    69421 accaaaacca aataccgatc cgtctgtaaa acgagtggag gcattgacat agacagccgc
    69481 cgcgtccact tcatttaaga aacgttgact agccgcataa ctatctgtca cgatcgtttc
    69541 tgaatgttta gtattatgcc gattgatatg tgcaattgct tcatccaaag aatcgaccac
    69601 tttgacagca aggatataat ctagatattc agtatcccaa tctgtgtctt ctgctaaagc
    69661 agccgtctct agatattcta acgcacgagc atccgcacgt aaagagaccc cgtgttctac
    69721 cagttctttt tcaatagctg gcaagaaaaa aggtgcgatc tcagaatgga tcagcaatgt
    69781 ttctgccgcg ttacagacag atggacggga agcttttgca ttgacgatga tgttcactgc
    69841 catagataac tgtgcttctt tatcgatata tacgtgatta tttcctgtac ctgtttcaat
    69901 gataggtacc gttgcctgtt ctttgactcg ttggattaat tttgctccgc ctcgaggaat
    69961 cagcacatct aagtattctg tcaatcgcat caactgtcct gcagtttcat gagaagtatc
    70021 atcgacaaac tggatggcat aaggagattc gccagcactg accagtactt gctgcaataa
    70081 ttggactaat aattgattcg agaagaatgc ttctttgcca ccgcgaagga taacggcatt
    70141 ccctgatttg aatgctagac tagctgcatc tgtcgtgaca tttggacgag attcgtaaat
    70201 gatgccgatc acgcctagtg gcacgcgctt tttgccgatt ctcaatccgt cggtgttcgt
    70261 ccacatctca tctacttttc caataggatc gtctaacgaa gcaatttttt cgatctctgt
    70321 agccatggca ttgattcgct caggtgtcag ctgcaagcgg tcttgcatcg tttcactgat
    70381 attgttttcg actgcttttt ctaagtcttt atgatttgct tcaatgatcg cttgttgatt
    70441 ctttttgatg gtttgtgcca tttgaacaag tagctcattt ttgcggtttg taggcataag
    70501 tgctagttgt tgcgcgctat cttttgcctg ttgtcctaat tgcatcaagt cagtcatttc
    70561 aagtcctcct taaataaagt acctacttct tttcctgcaa gaatatcaaa aataattgcc
    70621 ggattttttc catttgctaa aatcattgcg ctgtttgctt caaatatcct agaagcggct
    70681 tttaatttac tctgcatacc gcctgttcca aatgtgctac cagcaccacc tgcttgtgcc
    70741 agaatctttt catcgatttt atcgatatag cgatacatct gagcctcggg atttgtagtc
    70801 ggattatctg aataaaaccc gtcgatatca gaaaggacga tcaaaaggtc tgccgcgaca
    70861 agttttgcca tgatggctga cagctgatca ttatcgccaa attttgtcag atgatctaat
    70921 tcatccactg ctacagtatc gttttcgttc acgataggga tcgtgtccat aatcaaaagc
    70981 tgttccaatg tattcctcac atttttacga ctttcaggat aatctaccac gtctcttgtc
    71041 acgaggatct gtgccgtctg ttgattatat acactgaatc tttgattata gatattcatc
    71101 agttcggctt gaccaacagc cgccgcagct tgctgttcag gaatcaaagt cggacgttct
    71161 gacaaatgta atttatgcat accaacgcct acagctcctg atgagactaa gatcacttcc
    71221 tttccccgat ttctaagatc agaaaggaca aaagctaact gatcgattgc cgcaagattg
    71281 atatttccat tgggataaat caatgtactt gttcctactt tgacaataat tcgttttgca
    71341 tttttaattt ctttacgcat gtgacacctc aatcaaatat ccactcatta atttcgttct
    71401 attctacttg ttttgacgag taaagtcatg taaaaaatag tttttttgta caacttgcag
    71461 tcgtttggaa aaagaacgaa ttaatggttt actttaagat agcagaattt cttgaagaat
    71521 ttgtaaagaa agtcttttac agcctcggat atgttagaat aggagtagtt tatttaggga
    71581 ggaaaaccgg tgagaaaaag aggatttgaa gttatttcta aatataaaga tcaaaatatc
    71641 aatctaccgc agcgcgctac acatcatgct gctggctatg attttgaagc agcagaagag
    71701 gtcgtgattc caagtatctg gaaagcaggc tggaaaaagg ggttggaaac agtcgcagat
    71761 ggattgaaaa aagatgtatc agaaaatgaa aaacaattga aaccaaccct cgtgcctact
    71821 ggaatcaaag cttacatggg agaagatgaa tttttgcagc ttgctaaccg ttcaagcaat
    71881 ccgttgaagc ggtttttagt gttgacaaac ggagtaggtg tgattgacag tgattattac
    71941 aataatgagg caaatgaagg ccatatcatg ttccaattca ccaactttgg tttcaaggat
    72001 gtagtgatca aaaaaggcga gcgtatcggc caagggatct ttttgccgtt tttaaaagca
    72061 gatcaagatc aaacgactgc tcaacgtcaa ggcggatttg gttcttccag taaataaaac
    72121 gttaaagata cttaagcttg atttaatgtc tcatgattag gataaaagga ggcggtacaa
    72181 tggcaaaaaa atcaaaagta caatttgttt gccaaaattg cgggtatgtg tcgcctaagt
    72241 tcttgggacg ctgcccaaat tgcggaaaat ggaatacgat ggtcgaagaa atcgaacaag
    72301 atacaacgga ccgcagaact cggacaagtt tgacaggaga aaaagcaaaa ccaacaaaaa
    72361 tcgcagacgt cgtccctaaa aaagaacctc ggatcaaaac aaaattagaa gaactgaacc
    72421 gtgtgttagg cggaggtgtg gtgccagggt ctatggtctt gattggtggc gatccaggta
    72481 tcgggaaatc cactttgctg ttacaagttt ctcagcaatt agcagccata ggcggaaaag
    72541 tcttatatgt atccggcgaa gaaagcgcag agcagataaa attacgcgcg gaacgcctcg
    72601 gaagtatcaa cacagaattt tatctatacg ctgaaacaga tatgaacgaa atcagtcgtg
    72661 ccatcgaaca tatctcccct gattatgtta tcattgattc catccaaacg atgacgcagc
    72721 cagacatcac aagtgtggca ggaagtgtca gccaagtaag agaaaccact gcagaactac
    72781 tcaaaaccgc taaaacaaat gggatagcca tttttatcgt cggtcatgtt accaaagaag
    72841 gctcgatcgc cggcccaaga atgttagaac atatggtgga tacggtgtta tattttgaag
    72901 gtgaacaaca tcatagtttt cggattttgc gagcagtaaa aaaccgattt ggctcaacga
    72961 atgagattgg gatttttgaa atgcatgagc atggattgga agaagtcgct aatccttcac
    73021 agattttctt agaagaacgc ctagacggtg caactggctc tgctatcgtt gtggcaatgg
    73081 aagggacacg tccaattttg gtagagatcc aagcattagt cacacctact atgttcggaa
    73141 atgccaaacg aacaacaaca ggtcttgatt tcaatcgggt atctctgatc atggcagtgc
    73201 tggaaaaaag agctgggttg cttcttcaga atcaagatgc ttacttaaaa gctgcaggtg
    73261 gggtaaaatt aaatgaacca gcgattgatt tagctatcgc tgtcagtatt gcttcaagct
    73321 ataaagaaaa aggaacacag ccaacggaat gtttcatcgg tgaaattgga ctgactggag
    73381 aaatcagacg tgtcagtcat attgaacaac gagtcaaaga agtccaaaaa ctcggcttca
    73441 caaaagtata cctacctaaa aacaacttag gtaactggga agcgccaaaa ggaatcgaga
    73501 tagttggagt agcaactttg gctgaaacgt tgaagcgtgt gttccgctaa ctaataaatg
    73561 attgtcgaaa cggaggaaaa acgatgcaaa aacgtgtcat tactttgctg atgatcattg
    73621 tcggcgcaag cttgggtatt tcatttttac cctcagcttg gcaggcaatc ggtcaacagt
    73681 caaacggatg gctgaataat acatttacca acagcctgtt aggcgcactt attttctttt
    73741 tattatcttt gctgttggcc aaacatatct cagctattat caaacaagta gaacaaaaac
    73801 taagtgaggt cagcttgact tatcttctct tcggtgcgat cggtgcgatc atcggattga
    73861 ctttaggtgt tgtgatctcg acccctcttt acaatatgga aattccattc gtcaatagtg
    73921 tattgccgat tttactgatg atcatcttag gctatctggg attgagaatg ggaacgacac
    73981 gaatcgaaga atggaagaaa gtttttggtt cacgaagcaa aaaaatcgag caagaaacag
    74041 ctagtcaagt tgagagtcaa ctgttagaac gaaaatcagg agaaaatttc cataaatata
    74101 aaatcttgga tacaagcgtg atcattgacg gtcggattta tgatattaca aagactggat
    74161 tcttagaagg aaccttgatg atccctaatt ttgttcttta cgaactacaa tacatcgcgg
    74221 actcagggga tagtttgaaa cgtgtgcgtg gtcgtcgcgg attagatatc ctaaatgcct
    74281 tgcaaaaaga agagggtata tctgtcgaaa tgtacgatgg agactttgaa gatatcagtg
    74341 aagtagacag caaattgatc aaattggcta agcttttaga tggtgtcatt gtaaccaatg
    74401 actataactt gaataaagtc tctgaattcc aaaatgtacc tgttcttaat atcaatgcgt
    74461 tggcaaatgc cgtaaaacca gtcgtcatcc ctggtgaaac gatgaatgtt ctagtagtaa
    74521 aagccgggac agaacgccaa caaggagtag cctaccttga tgacggcacg atggttgtcg
    74581 tagaagatgg acagcattac atgaatgaac gtatcgaagt cgttgtaaca agtgcccttc
    74641 aaacagctgc tggacgcatg atctttgcta aacctgcaca tgcaggacga ggaatcgaca
    74701 ataaaaaaga gacagagaaa aagactaaac aatcaaaata aaaaagaaat tcagccaact
    74761 ggtaaggaaa ttcaaccagt tggctttact atttacctta gaaaaggtaa aatttgtgct
    74821 gagtggcttt aaataaatga tatttgagaa gccgtaactg acggaatttt tgctgttagt
    74881 caaagtctag tttgaaagaa gaggaagaaa atgacgaaag tacgcgtacg ctatgcacca
    74941 agtccaactg ggcacctgca tattggaaat gcaagaactg cattattcaa ttatttgttt
    75001 gcccgccaca atgatggaga ctttattatt cgtattgagg atactgacca aaaacgtaat
    75061 atcgaagatg gcgaaaaaag ccagttggaa aaccttgctt ggttgggaat gaattgggat
    75121 gaatctcctg aaaatcctgg agaatacggc ccttaccgtc aatctgaacg taaagaaatc
    75181 tatcaaccgc tgattgatca gctgcttgct agcaatcgag catacaaatg ctattgcaca
    75241 gaagaagaat tagaagcaga acgggaagca caacgcgcaa gaggagaaat gcctcattac
    75301 gcaggaacat gtgcgaactt gacacctgaa gaacaagcag aaaaagaagc acaaggtcta
    75361 gaatctgttg tacgtttccg tgtgcctcgc aatacagaat acgcatttac agatatggtg
    75421 aaaggcgcta tttcgttcga atccgataat atcggtggcg actttgtcat ccaaaaacgc
    75481 gatggtatgc caacttataa ttttgctgtt gcggtcgatg atcatttgat gaaaatcact
    75541 cacgttttac gtggagatga ccatattgcc aatacaccaa aacaactgat gatctatgaa
    75601 gcatttggct ggacaccacc aaccttcgga catatgacat tgatcatcaa cagtgaaaca
    75661 ggtaaaaaac taagcaaacg tgacgaatca atcttgcaat ttatcgaaca atatcgcgaa
    75721 ttaggctatc taccagatgc aatgttcaac tttattgcat tattgggctg gtcacctgtg
    75781 ggcgaagacg aaatcttcga acaccaagaa ttgatcaaaa tgttcgatcc aaaacgccta
    75841 agtaaatcac ccgcagcttt cgatgcgaaa aaactgcaat gggtaaacaa ccattacatc
    75901 aaagcaatgg atctagatgc atttactgac atgtgtctgc cttatctaat cgctgatgga
    75961 cgtgtagcag aaaacccaga tgaaaagaca atcgaatggg tgaagaagat cgttagcctg
    76021 tatcaacctc aaatgagcta tgctgcagaa atcgttgatc tgtctacgct atttttcaat
    76081 gaacatcctg tattgaatga agcagcaaaa gaagtacttg ctggtgaaac agttcctact
    76141 gttctccacg cattcaaagc acagctagaa caaatggaag tagtagatgc tccaactgtc
    76201 aaagctgcca tcaaagctgt tcaaaaagaa actggcgtaa aaggaaaaaa tcttttcatg
    76261 ccaatccgtg tagctgtatc aggacaaatg catggtccag aattgcctga aacgatcgaa
    76321 ttgcttggtc gtgaaaaatc attagcacat ttaaatcaag tactgaacta aacacgatga
    76381 acagaaaaaa gtaagcagct gatcattaca agagagatac cgggtggtgc aaggtatcaa
    76441 tgaatggttg ccgaaatgcc tctgggagtg gtcttggcga aacacagaaa acaggaaaaa
    76501 ctgttttata ggaaacaaag tagccaagaa cgtataacag gcgttagctg tcagagataa
    76561 tcattgatta tgaagaaaag tggaaccacg cggaaagcgt cttttgataa ggaaacttat
    76621 ttaaaaggcg cttcttttct ttataagaag atgatttcct acataaaatt cttacgaaag
    76681 gagtggaaag tcatgagttg gtttacccga gcgattgatg cagcaaagag aaacgatccg
    76741 gctgcacgtt cgacagctga gatcgttttg acataccctg gttttcatgc cttgttttgg
    76801 catcgtttct ctcatttttt atatcagcac cggctctttt tattggcgaa gatcaatgct
    76861 caattctggc gtttcctaac tggtgttgag atccatccag gagcaacgat tggaactggc
    76921 gtgttcatcg accatggaat gggaatcgtc attggcgaaa ccgcagaaat cgaggacgat
    76981 gtgattttat tccatggcgt aacattaggc ggcacaggaa aagaaacggg taaaagacac
    77041 ccaactgtta aacaaggagc catgctctct gcgaatgcgc agatattagg accggtaacg
    77101 atcggaaaaa acgccaaaat cggtgcaggt gccgttgtgt tgaaagatgt tccagacgac
    77161 gctacagcgg taggagtacc agcaaaagtc gttcgaatca aaggagaaaa agtgaggaga
    77221 tcagaatgat aaaaatctac aatacattga ccagaaaaaa agaagaattc atccctatac
    77281 aaaagggaca tgtcggtatg tatgtctgcg gaccgactgt gtataattat atccatatcg
    77341 gaaatgctcg tagcaccatt gctttcgata caattcgacg ctattttgaa tatcgaggct
    77401 ataaagtcga tttcgtatcg aatttcacgg atgtagacga taaaatcatc cgtacagcaa
    77461 acgaattagg tattacagca ccagaagttg ctgatcgttt catccgagca tttgaagaag
    77521 atacgaaagc gttgaatgtc gaacctgcta cactgcatcc aagagtcatg gatcatatgc
    77581 cagatatttt agcatttatt caagtgttgg ttgaaaaagg ctatgcttat gaatcttcag
    77641 gtgatgtgta ttaccgtacc cgtaaatttg aaaattatgg gaaattaagt gaccaatcca
    77701 tcgatgaact ggaagtcgga gcaagtcagc gtacagggga agaacagcag caaaaagaag
    77761 acccattaga tttcgcttta tggaaacaag caaaacctgg cgagatttct tgggattcac
    77821 catggggcaa aggacgtccc ggttggcata ttgaatgttc cgttatggca acaaaacatt
    77881 taggagaaac gatcgatatc catggtggcg ggcaagattt agaatttcct catcatgaaa
    77941 atgaaatcgc acaaagtgaa gcgaaaacag ggaaaaaatt cgctaattac tggatgcaca
    78001 atggctatgt aacgattggc gaagacgatg aaaaaatgag taaatctcta gggaatttca
    78061 tcactgtaca tgaaatgatc aaaaacgttg atccacaagt cttgcgtttc ttcatggcga
    78121 ccacacaata ccgtcgtccg atccgctaca gtgaaacgac gatgaaagaa gcagccacca
    78181 atttacaacg actgaagaat acgtttgaca atttgaaatt ccgccaagaa actgcctcag
    78241 agaatttggc agatgatgcg gataaaatcg ctttgttaga aaatttagaa cagcgattca
    78301 tcgaagaaat ggacgatgat tttaatgcag caaatgggat cacagtggtc tatgagcttg
    78361 ctaagtggct gaacacttat ctagaccagc cgaaagtttc tgccgctgtc ttggcacaag
    78421 gagaagcttt attctcgcaa tggctttcga tcttcggtat tttatttaca gcagaagagt
    78481 tattagatga agagattgaa caattgatcg aagaacgaaa tcaagcaaga agcaatcgtg
    78541 attttgtccg aagcgacgaa attcgtgatt tgttgaaaga aaaagggatc ttattagaag
    78601 atacaccgca aggaacccga tggagaagag aagcatgaga gattacacac aattgaacgg
    78661 tttagccttg gcttatgttg gagatgcgat ttacgaaatc tatgttcgtg accacctgat
    78721 tgatcaagga gaaacaaaac ccaataagct gcatcgtgcg gcaacgaatt atgtttctgc
    78781 gaatgcacaa gcctttttga ttcagcaaat gctggaagaa catcttttga cagaagaaga
    78841 agaactttat tatcgcagag gaagaaatgc taaaagccat acgagcgcaa aaaatgctga
    78901 tgttacgacg tatcgggtcg caacagggtt cgaagcgctc atgggctatc tgcatttgac
    78961 aaaacaaacc gagcggatgg aagaattgat ccgctggtgt attcagaaag ttggtgaaaa
    79021 aaatgggtaa agaaagaaca aacaaacatc aatcgaaacg tggatttgaa gacaaaaaac
    79081 aaaatagata ttcaaataaa aagcaaactg gacgaaatca gtcagtttct gatcaaaatg
    79141 aagaagcatt agaagataat ttcgtctttg ggcaccatgc cgcagtagaa gcgctacaac
    79201 aagggcgagg gaataaactc tttcttcaag aagattcacg aggagaaaaa gtagaagaac
    79261 tgaaagaaat cgcacgtgaa caagctgtgc cagtcaaatg ggttcctaaa caaaaattag
    79321 aaactttgag cgatcacggc gtgcatcaag gagtcgttct tgcgattaca ccttatgaat
    79381 atttatccct tgaccaactg attgaacgaa caaaagagaa aaaagaagac cccttctttt
    79441 tgattttgga tagcatcgaa gatccacaca attttgggtc gattttacgg acagcagacg
    79501 caagtggcgt cgatggtgtg atcataccaa aacaccgagc agttggaatc acacctatcg
    79561 taacaaagac ttccactggg gcagtcgaac atgtccctgt tgcacgcgtg acgaatttgg
    79621 tacaagctgt gcaacagctc aaagaaacag gattttgggt atttggcaca gatatgaccg
    79681 gcacaaactt ccaaaaatgg aatgccaaag gatcaattgc tttagtcatt ggtaatgaag
    79741 gcaaaggaat gagtccagga ttgaaaaaag aaatggatga attgctgacg atcccgatga
    79801 ccgggcacgt ccagagctta aatgccagtg ttgctgctgg tttgcttatg tatcaggctt
    79861 tttcccaacg tcagggggag tagaccatga aaaaacagct gttgattgta gacggctaca
    79921 acatgattgg ttcgtggccg gaacttgtcc agcttaaaaa gcaggacaaa atggcggatg
    79981 caagagaagc tcttttacat cgtttatcaa attatgcgaa atatgaagga atggaagtca
    80041 tcgttgtctt cgatgcccaa ctagttccgg gcatccaaca aaattataag aaataccagt
    80101 tggatgttgt ttttacgaaa gaagacgaaa cggctgacag ttatatcgag agaatcgctg
    80161 gtcagctgaa tgaccgtttg acacaagtga cagtggcgac aagcgattta gcagaacagt
    80221 ggatgatctt ttcccaagga gcattacgaa cttctgctta tgaattatat aagacgataa
    80281 aaaaaacgga aaaaacaata gcccaagaaa ataaagatat gcattttatt gatttccgac
    80341 gtaattctcc atggaatgaa gagcagttaa aaaaattgtc c
//
LOCUS       DACDUU010000005        72502 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.005, whole genome shotgun sequence.
ACCESSION   DACDUU010000005 DACDUU010000000
VERSION     DACDUU010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 72502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.005"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            313..3147
                     /locus_tag="IWL30_000317"
     CDS             313..3147
                     /locus_tag="IWL30_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9705112.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            3338..3829
                     /locus_tag="IWL30_000318"
     CDS             3338..3829
                     /locus_tag="IWL30_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAP9705113.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            3932..4921
                     /gene="manX"
                     /locus_tag="IWL30_000319"
     CDS             3932..4921
                     /gene="manX"
                     /locus_tag="IWL30_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAP9705114.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            4954..5757
                     /locus_tag="IWL30_000320"
     CDS             4954..5757
                     /locus_tag="IWL30_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAP9705115.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            5778..6692
                     /locus_tag="IWL30_000321"
     CDS             5778..6692
                     /locus_tag="IWL30_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAP9705116.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            6775..7149
                     /locus_tag="IWL30_000322"
     CDS             6775..7149
                     /locus_tag="IWL30_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAP9705117.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            7288..7533
                     /locus_tag="IWL30_000323"
     CDS             7288..7533
                     /locus_tag="IWL30_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9705118.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            7705..8769
                     /locus_tag="IWL30_000324"
     CDS             7705..8769
                     /locus_tag="IWL30_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP9705119.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            9015..9440
                     /locus_tag="IWL30_000325"
     CDS             9015..9440
                     /locus_tag="IWL30_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9705120.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            9433..9882
                     /gene="nusB"
                     /locus_tag="IWL30_000326"
     CDS             9433..9882
                     /gene="nusB"
                     /locus_tag="IWL30_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAP9705121.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            9979..10833
                     /locus_tag="IWL30_000327"
     CDS             9979..10833
                     /locus_tag="IWL30_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAP9705122.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            10834..12171
                     /locus_tag="IWL30_000328"
     CDS             10834..12171
                     /locus_tag="IWL30_000328"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAP9705123.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            12176..12400
                     /locus_tag="IWL30_000329"
     CDS             12176..12400
                     /locus_tag="IWL30_000329"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAP9705124.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            12402..13292
                     /locus_tag="IWL30_000330"
     CDS             12402..13292
                     /locus_tag="IWL30_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP9705125.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            13301..14119
                     /locus_tag="IWL30_000331"
     CDS             13301..14119
                     /locus_tag="IWL30_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAP9705126.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            14226..14675
                     /locus_tag="IWL30_000332"
     CDS             14226..14675
                     /locus_tag="IWL30_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP9705127.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            14693..16375
                     /gene="recN"
                     /locus_tag="IWL30_000333"
     CDS             14693..16375
                     /gene="recN"
                     /locus_tag="IWL30_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAP9705128.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(16430..16546)
                     /locus_tag="IWL30_000334"
     CDS             complement(16430..16546)
                     /locus_tag="IWL30_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAP9705129.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(16619..17575)
                     /locus_tag="IWL30_000335"
     CDS             complement(16619..17575)
                     /locus_tag="IWL30_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP9705130.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            17746..18114
                     /locus_tag="IWL30_000336"
     CDS             17746..18114
                     /locus_tag="IWL30_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAP9705131.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            18284..18715
                     /gene="mraZ"
                     /locus_tag="IWL30_000337"
     CDS             18284..18715
                     /gene="mraZ"
                     /locus_tag="IWL30_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAP9705132.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            18736..19695
                     /gene="rsmH"
                     /locus_tag="IWL30_000338"
     CDS             18736..19695
                     /gene="rsmH"
                     /locus_tag="IWL30_000338"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAP9705133.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            19700..20101
                     /gene="ftsL"
                     /locus_tag="IWL30_000339"
     CDS             19700..20101
                     /gene="ftsL"
                     /locus_tag="IWL30_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAP9705134.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            20102..22288
                     /locus_tag="IWL30_000340"
     CDS             20102..22288
                     /locus_tag="IWL30_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP9705135.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPSED
                     PDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYRNL
                     EADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVTGW
                     SKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            22314..23276
                     /locus_tag="IWL30_000341"
     CDS             22314..23276
                     /locus_tag="IWL30_000341"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAP9705136.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            23290..24651
                     /locus_tag="IWL30_000342"
     CDS             23290..24651
                     /locus_tag="IWL30_000342"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAP9705137.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVSECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            24677..25765
                     /gene="murG"
                     /locus_tag="IWL30_000343"
     CDS             24677..25765
                     /gene="murG"
                     /locus_tag="IWL30_000343"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAP9705138.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            25780..27000
                     /locus_tag="IWL30_000344"
     CDS             25780..27000
                     /locus_tag="IWL30_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAP9705139.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            27156..28481
                     /gene="ftsA"
                     /locus_tag="IWL30_000345"
     CDS             27156..28481
                     /gene="ftsA"
                     /locus_tag="IWL30_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAP9705140.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            28504..29745
                     /gene="ftsZ"
                     /locus_tag="IWL30_000346"
     CDS             28504..29745
                     /gene="ftsZ"
                     /locus_tag="IWL30_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAP9705141.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            29762..30439
                     /locus_tag="IWL30_000347"
     CDS             29762..30439
                     /locus_tag="IWL30_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAP9705142.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            30453..31052
                     /locus_tag="IWL30_000348"
     CDS             30453..31052
                     /locus_tag="IWL30_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAP9705143.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            31067..31354
                     /locus_tag="IWL30_000349"
     CDS             31067..31354
                     /locus_tag="IWL30_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAP9705144.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            31403..32185
                     /locus_tag="IWL30_000350"
     CDS             31403..32185
                     /locus_tag="IWL30_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAP9705145.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            32206..32913
                     /locus_tag="IWL30_000351"
     CDS             32206..32913
                     /locus_tag="IWL30_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAP9705146.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            33175..35973
                     /gene="ileS"
                     /locus_tag="IWL30_000352"
     CDS             33175..35973
                     /gene="ileS"
                     /locus_tag="IWL30_000352"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAP9705147.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(36022..37548)
                     /locus_tag="IWL30_000353"
     CDS             complement(36022..37548)
                     /locus_tag="IWL30_000353"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAP9705148.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(37563..38210)
                     /locus_tag="IWL30_000354"
     CDS             complement(37563..38210)
                     /locus_tag="IWL30_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAP9705149.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(38394..38723)
                     /locus_tag="IWL30_000355"
     CDS             complement(38394..38723)
                     /locus_tag="IWL30_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAP9705150.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            38900..39628
                     /gene="sfsA"
                     /locus_tag="IWL30_000356"
     CDS             38900..39628
                     /gene="sfsA"
                     /locus_tag="IWL30_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAP9705151.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            39644..40657
                     /locus_tag="IWL30_000357"
     CDS             39644..40657
                     /locus_tag="IWL30_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9705152.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            40657..41934
                     /locus_tag="IWL30_000358"
     CDS             40657..41934
                     /locus_tag="IWL30_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP9705153.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            41997..44699
                     /locus_tag="IWL30_000359"
     CDS             41997..44699
                     /locus_tag="IWL30_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9705154.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            44851..45168
                     /locus_tag="IWL30_000360"
     CDS             44851..45168
                     /locus_tag="IWL30_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9705155.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            45198..45518
                     /locus_tag="IWL30_000361"
     CDS             45198..45518
                     /locus_tag="IWL30_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9705156.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            45626..47086
                     /locus_tag="IWL30_000362"
     CDS             45626..47086
                     /locus_tag="IWL30_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9705157.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            47154..47375
                     /locus_tag="IWL30_000363"
     CDS             47154..47375
                     /locus_tag="IWL30_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705158.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            47406..47588
                     /locus_tag="IWL30_000364"
     CDS             47406..47588
                     /locus_tag="IWL30_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAP9705159.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            47588..48001
                     /locus_tag="IWL30_000365"
     CDS             47588..48001
                     /locus_tag="IWL30_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAP9705160.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            48124..49305
                     /locus_tag="IWL30_000366"
     CDS             48124..49305
                     /locus_tag="IWL30_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9705161.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            49694..49767
                     /locus_tag="IWL30_000367"
     tRNA            49694..49767
                     /locus_tag="IWL30_000367"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:49728..49730,aa:Arg,seq:cct)
     gene            complement(49836..50975)
                     /locus_tag="IWL30_000368"
     CDS             complement(49836..50975)
                     /locus_tag="IWL30_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9705162.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(51274..51909)
                     /locus_tag="IWL30_000369"
     CDS             complement(51274..51909)
                     /locus_tag="IWL30_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705163.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(52022..52657)
                     /locus_tag="IWL30_000370"
     CDS             complement(52022..52657)
                     /locus_tag="IWL30_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HAP9705164.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(52691..53152)
                     /locus_tag="IWL30_000371"
     CDS             complement(52691..53152)
                     /locus_tag="IWL30_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HAP9705165.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(53282..53713)
                     /locus_tag="IWL30_000372"
     CDS             complement(53282..53713)
                     /locus_tag="IWL30_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP9705166.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(53731..54051)
                     /locus_tag="IWL30_000373"
     CDS             complement(53731..54051)
                     /locus_tag="IWL30_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9705167.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVNLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            54350..55126
                     /locus_tag="IWL30_000374"
     CDS             54350..55126
                     /locus_tag="IWL30_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP9705168.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            55141..55344
                     /locus_tag="IWL30_000375"
     CDS             55141..55344
                     /locus_tag="IWL30_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9705169.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            55360..55698
                     /locus_tag="IWL30_000376"
     CDS             55360..55698
                     /locus_tag="IWL30_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAP9705170.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            55685..55864
                     /locus_tag="IWL30_000377"
     CDS             55685..55864
                     /locus_tag="IWL30_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705171.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(55907..56377)
                     /locus_tag="IWL30_000378"
     CDS             complement(55907..56377)
                     /locus_tag="IWL30_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705172.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            56464..57162
                     /locus_tag="IWL30_000379"
     CDS             56464..57162
                     /locus_tag="IWL30_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP9705173.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            57340..57681
                     /locus_tag="IWL30_000380"
     CDS             57340..57681
                     /locus_tag="IWL30_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705174.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            57674..58345
                     /locus_tag="IWL30_000381"
     CDS             57674..58345
                     /locus_tag="IWL30_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP9705175.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            58351..59037
                     /locus_tag="IWL30_000382"
     CDS             58351..59037
                     /locus_tag="IWL30_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705176.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            59040..59789
                     /locus_tag="IWL30_000383"
     CDS             59040..59789
                     /locus_tag="IWL30_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9705177.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            59801..60070
                     /locus_tag="IWL30_000384"
     CDS             59801..60070
                     /locus_tag="IWL30_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705178.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            60232..60534
                     /locus_tag="IWL30_000385"
     CDS             60232..60534
                     /locus_tag="IWL30_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705179.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            60531..60692
                     /locus_tag="IWL30_000386"
     CDS             60531..60692
                     /locus_tag="IWL30_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP9705180.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            60689..60994
                     /locus_tag="IWL30_000387"
     CDS             60689..60994
                     /locus_tag="IWL30_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705181.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            60994..61350
                     /locus_tag="IWL30_000388"
     CDS             60994..61350
                     /locus_tag="IWL30_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAP9705182.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            61310..61555
                     /locus_tag="IWL30_000389"
     CDS             61310..61555
                     /locus_tag="IWL30_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705183.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            61552..61971
                     /locus_tag="IWL30_000390"
     CDS             61552..61971
                     /locus_tag="IWL30_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAP9705184.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            61968..62525
                     /locus_tag="IWL30_000391"
     CDS             61968..62525
                     /locus_tag="IWL30_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP9705185.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            62522..62818
                     /locus_tag="IWL30_000392"
     CDS             62522..62818
                     /locus_tag="IWL30_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705186.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            62895..63308
                     /locus_tag="IWL30_000393"
     CDS             62895..63308
                     /locus_tag="IWL30_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP9705187.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            complement(63766..64041)
                     /locus_tag="IWL30_000394"
     CDS             complement(63766..64041)
                     /locus_tag="IWL30_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705188.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            64495..64701
                     /locus_tag="IWL30_000395"
     CDS             64495..64701
                     /locus_tag="IWL30_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705189.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            64897..65064
                     /locus_tag="IWL30_000396"
     CDS             64897..65064
                     /locus_tag="IWL30_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP9705190.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            65090..65434
                     /locus_tag="IWL30_000397"
     CDS             65090..65434
                     /locus_tag="IWL30_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP9705191.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            65439..65720
                     /locus_tag="IWL30_000398"
     CDS             65439..65720
                     /locus_tag="IWL30_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705192.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            65823..66137
                     /locus_tag="IWL30_000399"
     CDS             65823..66137
                     /locus_tag="IWL30_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAP9705193.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            66115..67809
                     /locus_tag="IWL30_000400"
     CDS             66115..67809
                     /locus_tag="IWL30_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP9705194.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            67829..69007
                     /locus_tag="IWL30_000401"
     CDS             67829..69007
                     /locus_tag="IWL30_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP9705195.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            68970..69656
                     /locus_tag="IWL30_000402"
     CDS             68970..69656
                     /locus_tag="IWL30_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP9705196.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            69656..70816
                     /locus_tag="IWL30_000403"
     CDS             69656..70816
                     /locus_tag="IWL30_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP9705197.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            70826..71701
                     /locus_tag="IWL30_000404"
     CDS             70826..71701
                     /locus_tag="IWL30_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705198.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            71698..72009
                     /locus_tag="IWL30_000405"
     CDS             71698..72009
                     /locus_tag="IWL30_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705199.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            71999..72352
                     /locus_tag="IWL30_000406"
     CDS             71999..72352
                     /locus_tag="IWL30_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP9705200.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
BASE COUNT        24528 a        11896 c        15226 g        20852 t
ORIGIN      
        1 ctttgtcttt ttacacttaa tatttttttg ccaatttgga atcaggatgt tttgataact
       61 atcgttttca attgatggaa gctatattgt ttgctatcag tgaaataatt atatggtaaa
      121 cagtgttctt tttcttgttt ccattataaa aatcgtatcg ggatttttcg ttcttttaaa
      181 ttgaacattt ctatctatcc gcgtgttatc aacaaaacaa aatcaggtaa atagttgacg
      241 aaacagaaat aaatacatag tattatgttt tgaagcctaa gtacaatagt ctatagaaaa
      301 ggagcgatac cattggcaaa acgcattgac cgaatttata tgtatgtcaa agaaaaaacc
      361 gctcatttaa caaaggcaga atatgatcaa ggagtaacaa cacaggaaat tgctgaagtg
      421 ttagggattc agcgaacaaa tagcagtaaa gatctgaatc agctagtgcg cgaaggaaaa
      481 ctgataaaga cagacggacg accagtacgt tacatatatc aaacactcgt tacgcacgta
      541 aagccagtta caaaacatgt agtcagttac aaagaaacgc cggtctctga agaaaaaaat
      601 acactccaaa tagatactaa ggatatcttt gcaaaaatca ttggagctaa cggcagcatg
      661 aaaaattcag tggaacaggc aaaagctgcg attttgtatc cgccaagagg attaaattgt
      721 ttgattacag gacctaccgg atcaggaaag acttattttg cacacgcgat gtatcatttt
      781 gcaaaagcgc atcatgtaat agatgaggaa acggaactga ttgttttcaa ttgtgctgat
      841 tatgccaata accctgagct tttgatgagt catttatttg ggtacgcaaa aggtgctttt
      901 acaggcgcag atgaagaaaa aatggggatt atcgatcaag cggatggcgg tatgctgttt
      961 ttggatgaga ttcaccggtt gccgcctgaa ggtcaagaaa tgatctttta ctttatggat
     1021 catggaactt acagtcgtct tggggaaaca acgaaatccc atgaagcaaa tgtacggatc
     1081 gtaggtgcga ccacagaaga cccaggttct tctctattag aaacttttgt tcggcgtatc
     1141 ccaatcaata tcaaacttcc ggcttttgaa aaacgtcctg caaatgagaa agtcgattta
     1201 gtaaaaatca tgattgccca cgaagcaaac cgcacgcagc gaaagatttc tttgacagaa
     1261 gatgtagcaa aagcattgat cggcagtgtg acctatggaa atataggaca attgaagtca
     1321 aatatccagt tagtatgtgc acgaggattt ctaaatcata tgcaatcacc cgaaatctcg
     1381 atcacaatcg atgatttgac agaagggatt cgtagcggat tgattcaatt agcaagcaac
     1441 cgcagtgcaa tggcagagct ttctaaaata ttggagccaa agataactgt agcaccaaat
     1501 gatacgatca tgaaaatcca atcggactcc tatgagttac cttataatct atatgatatc
     1561 atcggagata aagcagcttt actgaagtcg gatggtttgg atcaagaagc aatcaatcat
     1621 ttcatttcta cagatatcaa catccatctg aagtctttct ataaagacca tggtttttct
     1681 tttaatgcag acagcaaatt agcagaattt gtagatccga aaatcataga agtgaccaat
     1741 cagatttatg taatggtcaa aagatcttta ccatatgagt ttcagcaaaa ctttatttat
     1801 gctatgagct tgcatatcag ttcttttttg aaaaaaatca acttaggaga agaacggcat
     1861 acgaatgata acatccgtga gatggctttt gattttccag atgaatatgc tgttgcgaaa
     1921 gaagtgcgta gttatatcga aacgtatttt caagtggaga ttccagaaag tgaagactat
     1981 tacttgaccg ttctactcgt ctcgttacgg gcaaatcaag caagcggcag aatcggtgta
     2041 gtcgttgcag ctcacggaaa cagcacagca agcagtatgg tccaagttgt ccaacagttg
     2101 ttagatgcag agaacgtacg agcagtagac atgcctttgg atatggatcc gaaaacagca
     2161 ttggaccgaa ttgaacgcaa tgtacaagaa gtagatgaag gaagcggggt catactctta
     2221 gtagatatgg ggtcccttgc gtcgtttaat tcgcaaattc aaagagacac tggaattcca
     2281 gtacgtactg ttgatatggt aacaacttct ctagttttgg aaactgtacg aaaggtctca
     2341 gtattaggga cagatctaga tatgttgcat gaatcattga aaaattttcg aggttacgca
     2401 gaaatatctt ctgtagaatc tgttcaggaa gcaaaaccta ttcataagaa aaaagctgtt
     2461 ttggctatct gcgcatctgg cgaaggtaca gcacaaagaa tcaaagaatt gatcgaacga
     2521 gcggtaaaca agcgtcaaga aacagaatta gaagttctag ctttgtcgat tgtggaagtg
     2581 aaagaagaac tgccagttat ccaaaaagaa tatcagatca tagctactac tgggattact
     2641 gatttgaata tccaagcccc atttattcct ttagaacgtt ttattgatca aaatatcgaa
     2701 gtcattttgg atcagttgct gatggaatca gaattggagg aaactgaatt catctcccta
     2761 gatgaagagt cagctaaaaa tacgtgtgtt gagtttatct ctgataactt catctttatt
     2821 aatggtagta agttaatcga tccaatgtgg caattttcta cacaaatctc tcagacgacg
     2881 ggaatcggcg atgaagaata tggattcaaa atcaacctag tgatgcacac agcaggaatg
     2941 atcgaacgta ttattcggaa tgaaccattg actgtggaag aaaacgaatt gacaaataca
     3001 acgaatgatc ctctttatag ccaattggca gcctcagttg ttctgttgga agatcagata
     3061 aaagtaaaag tgccgataga agaaatgtat tatctgctac gtctagtcca taatcagtta
     3121 gataaaaaag aatacactgt gccatgaata cacaaaaacg atacactttt atagtgtatc
     3181 gtttttgtgt attggttaag aatcctttaa attcaattag tgtatcgtaa aaaagttggc
     3241 acgctttttg cttatagtag tgtgtgctag gaggtggttg ttcacacaga aatgataggg
     3301 gtttctgtga agtagtgagg aagaaggagt tttgcagatg gatattcgat tggcacgtat
     3361 agatgatcgt ttaatccacg ggcaagtcgc aacagtttgg tcgaagcaaa caggtgttga
     3421 acgtatcatt gtgatcagcg atgtagtcgc tcaagataaa ttgagaaagt ttctactaaa
     3481 agaagctgca ccgccgggga tcacagcaaa tgtaataacc gtgcagaaaa tgattcaaat
     3541 ctatcatagt ggattattat cacgagcaaa agtgatgctt ttattcacca atccccaaga
     3601 tgttgaagcg gttgtccgct gcggtgtaca gttgaagtcg ttgaatatcg gcggtatgag
     3661 ttttactgaa ggcaaatcaa tgattaccaa ttttgtttca gtaaatcaac aagatattga
     3721 ttcctttcgt tacttgaaca gtcaagaagt ggagttagag attcgaaaag tacctgctga
     3781 caggaaaatc cttttgatgg aactactagc gaaaatcaaa aaggtgtagc aagaatggtt
     3841 gttggaaaag gtcaaataat tctgttgtaa tggttagcaa ttattaatat ttttgaattt
     3901 gttacaacaa aagtaaatag gaggtataaa tatggtaggg attatcctag caagtcatgg
     3961 acaatttgct gaaggaatct tacaatccgg ttcaatgatc tttggcgaac aagaaaacgt
     4021 caaagccgtg attttgaaac caagtgaagg tccagatgac ttgagagcca aattggaaga
     4081 agcggttgct tcttttgata atcaagacga agtgttattc ttagttgatt tatggggcgg
     4141 aacaccgttc aatcaatcaa acacattatt cgaagagcac aaagataaat gggcaatcgt
     4201 cagcggtttg aatttaccaa tgttgattga agcctacgca tcacgctttt caatggaatc
     4261 tgctcatgaa attgcagcac acatcattga aacagcaaaa gacggtgtaa aagtaaaacc
     4321 ggaagaatta gaaccggcag aagcaccgaa agcagcagtt gaagacgcac aacctaaagg
     4381 agcacttcca gaaggaactg tggtcggaga cggaaaaatc aaatatgtat tagcacgtgt
     4441 tgactcacgt ctgttacatg gacaagttgc gacagcttgg acaaaagcag tgcaaccaaa
     4501 ccgtattatc gttgtttcag atgcagtatc aaaggatgat ctgcgtaaga gactaatcga
     4561 acaagctgct cctccaggtg taaaagcaaa tgttattcct attagcaaaa tgatcgaagt
     4621 agcaaaagac cctcgttttg gaaataccaa agcattgctt ttatttgaaa atcctgaaga
     4681 cgtattgaca gctgttgaag gtggcgtaga catcaaagaa ttaaatgttg ggtcaatggc
     4741 gcattcggtc ggtaaagtcg ttgtaagtaa agtattgtct atgggacaag aagacgttga
     4801 agcgtttgag aagcttgaac aaaaaggtgt gaaattcgat gtacgtaaag tacctaatga
     4861 ttcacgcgat aacatggatg atattttgaa aaaagcaaaa gctgaattag caaaagcgta
     4921 ataaataata ttaaaattta taggaggctt atcatgtcta ttatttcaat aattttagtc
     4981 gtattagttg cctttctagc tggtatggaa ggtattcttg atgaatttca atttcatcaa
     5041 ccactagtgg catgtacatt gatcggttta gttaccggta acctcgaagc tggtatcgta
     5101 cttggcggta cgcttcagat gatcgcttta ggttgggcaa atatcggtgc cgctgtagca
     5161 ccagatgctg cattagcatc agtagcatca gcaattattt tagttttagg tggacaaggt
     5221 gttaaaggtg ttccttctgc gatcgcgatt gccgttcctt tggctgttgc agggttgttc
     5281 ttaacaatga tcgtacgtac aatcgctgtt ccaatcgttc atttgatgga tgcggctgct
     5341 gaagaaggaa acatccgtaa ggttgaaatg tggcacatca ttgctgtatg tttacaaggg
     5401 gttcgtatcg cggtcccagc agcagcgttg ctatttatcc cagcacaaac tgtgcaatca
     5461 ttccttgaat caatgccagc atggttaaca gatggtatgg ctatcggtgg gggaatggtc
     5521 gtagctgtag gttacgcttt ggtaatcaac atgatggcta caaaagaagt atggccattc
     5581 ttcgttattg gtttcgttgt agctgcaatt tctcaattaa cattgattgc tctaggtgct
     5641 ttaggtgttg cattagctct tatttacttg aacctttcta aaatgggtgg ttcatcaaat
     5701 aatggaggcg gaagcaactc tggtgaccct ttaggcgaca ttttgaatga ctattaatct
     5761 tgaaggagga aaataaaatg gcagaagaaa aaatcaaatt atcaaaaaaa gatcgtttag
     5821 ctgttgcgtg gcgctctacc ttcattcaag gttcatggaa ctacgaacgt atgcaaaacg
     5881 gtggctgggc attttctatg attccagcaa tcaaaaaatt atacaaaaca aaagaagaac
     5941 gttctgcagc attaaaacgt cacttggaat tctttaatac acacccatac attgcttctc
     6001 caattttagg ggttacattg gctctggaag aagaacgtgc gaatggcgcg cctgttgatg
     6061 atgtagcgat tcaaggggtt aaagtaggta tgatgggtcc tttagctggt gttggtgacc
     6121 cagtgttctg gtttacagta cgtccaatgc taggtgcact aggtgcttca ttagctatgg
     6181 gaggaaatat ccttggacca atcatcttct tcttaggttg gaacattatt cgttgggcat
     6241 tcatgtggta tacacaagaa tttggctaca aagctggttc taaaattact gatgaccttt
     6301 ctggcggatt gttacaagac gtaacaaaag gtgcttcaat tttagggatg ttcgtattag
     6361 ctgcccttgt acaaagatgg gtatcaatca aattcttacc agttgtatca actgtaaaat
     6421 tagataaagg tgcttacatc gaatgggata aattgccttc aggtggtgaa ggaatcaaga
     6481 gtgcatttga acaagttaat aatggcttag ccctttctcc tgaaaaggta acaacattac
     6541 aagacaactt agaccaattg attcctggtt tagctgcatt aggattaaca ttgttatgta
     6601 tgtggttgct taagaaaaag gtttctccaa tcgttatcat ccttggttta ttcgtagtcg
     6661 gtgttgttgg acacgtcatc ggacttttat aatccataaa aatatagctc gggcgttgtc
     6721 ccgagctttt tttatatagt atactgttca taaacaaata tctggaggga gaaaatggtt
     6781 caatcaatca atacaaaagt agacttggta attgatgcta cttcgtttac gggtctgaca
     6841 gattacgggc aaatcatgat aggtgataat ggttttgagt tttaccatgc gcgagataga
     6901 cgcaaatata tccaaattcc atgggaagaa gtggattatg tactagcatc agtcatgcta
     6961 aagggaaaat ggattcctcg atatgccatt caaacgaaaa aaaatggtac atacacattt
     7021 tcttccaaag aacctaaaaa agtgcttcgt gcgatcagag aatatgtaga tgaagataga
     7081 atggtacaat cattgagttt ctttgatgta gtgaaacgtg gtattcgtag tatgtttaaa
     7141 cgcaagtaaa aaataaagaa aacttctaag aggaggtttt ctttattttt ttactttgca
     7201 gaagaaaata tgaaatagca aaaccaatta tttgtcccct ttaaatccca tatgctaaaa
     7261 ttgattagca aagaaggagg ttatattatg agttggttat ggacattgat tgttggagcc
     7321 gtgatcggag caattgcagg tgcgatcacc agtaaaggaa attcgatggg ctgtgtaacg
     7381 aatatcattg ctggactagt cggttcaagt attggacaag cgattttcgg cgaatgggga
     7441 ccaaaagctg caggtatggc acttattcca tctattttag gcgcagtgat tttggtagca
     7501 gttgtctcat ttttctttgg aaaacgatca taagttttta caaaatagta aactaaaagg
     7561 agttgcagcg agactgtgac tcctttttgg ctttatttat ttaaaataaa aagaagtttt
     7621 ccggaaattt ttatttaact gtcgtgtacg aagaaaagtt tgctataatg gaaaaaagcg
     7681 tgcaaagaaa gagggagttt cattatgatg ctacgtgtag aaaaattgcg caaaaaaatg
     7741 caagaagaaa atttggattc atttttggtt accagtccgt acaatcttcg ttacttaacg
     7801 aactttacag gtacgacagg attagctgtt attactttgg aaaaagcatt ttttattact
     7861 gattttcgtt ataccgagca ggcggcagcg caggctcaag ggtttgaaat tattaaaaat
     7921 gtcggaccta tttttgatga ggtagcaaac ttagtacgca aagaagaact gcactcatta
     7981 gcttttgagg aaacaacagt ctcattttta gaatattcgg ttttagaaga aatcatcgat
     8041 gcggaattag tacctgtttc acagatgatc gaagaactca gagaagtgaa agatgaagaa
     8101 gaaatcgcta ttatcgaaaa agcatgtcat attgcggata tggcatatga tcacatcctg
     8161 aaaatgatcc aaccgggcat gactgagatt gaagtcgcaa atcaactaga cttttatatg
     8221 cgttctcttg gcgcttcagg tgtttctttt gatacgatcg ttgctagcgg attgcgttcg
     8281 gctatgccgc atggtgtagc aagcaaaaaa atcatcgaac aaggtgactt gatcacattg
     8341 gactttggtt gctattacga agggtacgtt tccgatatga cacgtacatt cgctattggt
     8401 gatcctgggg aaaaattaaa agaaatttat caaatagtct tagatgctca attagcagtt
     8461 attgatgctg caaaaccagg aatgactggt gtccaattag atgcagttgc tagagattat
     8521 atttcaaaac atggttacgg ggaagcattt ggacattcta caggacatgg gatcggttta
     8581 gagatccacg aaggaccaaa cgtttcttct cgtgcagaaa agcaatttgt accaggaaat
     8641 gtgatcactg atgaaccagg gatctactta ccaggaatcg gtggagtacg tattgaggat
     8701 gacttgttga tcacagaaga tggaaatcgt gtgttgactc gttcaccaaa agaattgatc
     8761 atcttatagt ttcatcaata gaagaagtaa gcgtaaaaat cagtgttcac agactttgtt
     8821 atagaaaatc actcgattta atgatacact atagaagaac ttttaagctg taagagataa
     8881 taaagttggg tgttttctcc aattcatagc agtttgcagc tgatcggcgg cagttttctt
     8941 accacgtgat cactatgccg catcatagag aattcggatg tatctcgtgc ttaggcaaag
     9001 ggaggaataa ataaatggca gatgaaaaaa atttgatttt aaacgcaaac caagatttag
     9061 gagagatcgt gatcgctcct gaagtcatcg aagtaattat cgggattgca gcttctaaag
     9121 tagaaggcgt atacggaatg cgcggaactt ttgccagcaa tgtcactgaa ttattaggac
     9181 gtgcagctca cggaaaaggt gtttacttag caaacgagga agatggcttg aaagtagatc
     9241 tttactgtta tctagaatat ggtgtttcag ttcctaaagt agcaatggaa atgcaggaac
     9301 gggtcagcca gcaagtcctt ttcatgacgg atatcgattt atctgaagtc aatatccatg
     9361 tagtagctgt tgttcctgaa aaattagaac aaccaacaat cgaagaactt tttgaagacg
     9421 aagaggaaga gaatgaataa agaattatca agacacgaga ttcgggaaat ggcattgcag
     9481 gctttatttc cattagactt caatgcagac ctaacaaaag aagatgcgat tttcaacgca
     9541 atcgaattag accaccgcga catgatcaac gaagatgaat cagagtttgt tcctgtttat
     9601 cttgatacgc tagttggcgg tgtctgtgca aaaaaagatg aattggataa agtgattgaa
     9661 aaacatttaa aaaacaattg gcacatcaat cggatctcaa aaatggattt ggtgatcttg
     9721 cgtatcgcga tttttgaaat gacttatgtg acagatgtac cagcggctgt tgctttaaat
     9781 gaagcaatcg agttggcaaa aacatttagt gatgaccgtt cacgtaaatt cgtcaacggt
     9841 gttttatcca atgtattgaa agaattagaa gctggggcgt aagtcctagc ttttttctat
     9901 gaatcatcat aaacctgtta taaatcggga aaattctatg gtagaatgaa aacacaaaaa
     9961 atatctggga gtggttagat ggcagaattg attaacggaa aagaattagc tgaaaaaatg
    10021 caggcagaga ttgctgaaaa aatccaaaaa ttgaaagata atggtatcca tcccggatta
    10081 gtcgttttat tggtagggga aaatccagcc agccaaatct atgtaagaaa taaagaacgt
    10141 gtagcaaaag aaatcggaat ccattcattg gtagagcgtt atcctgagac gatttctgaa
    10201 gacgaactac ttgcagaaat tgaaaaatac aatcaagatc ctagttttca tggtatattg
    10261 gtccaattac cattgccaaa gcatatagat gaagaaaaga tcctgttagc tatccgccct
    10321 gaaaaagacg tagacggttt tcatccgatg aatttaggca gactccttgc gggtaaaccg
    10381 gacaagattc cttgtactcc atacggcatc atgaaaatgt ttgaagctta ttctatcgat
    10441 ccggcgggta aacgagcagt agttatcgga cgaagcaata tcgtcggtaa accgatggct
    10501 cagctactct tgatggcaga tgcaactgtt actgttgctc attctaaaac cgaaaatctg
    10561 gctgaactgg cacaagaagc agatatttta gtcgtagcaa taggacgtgg acattttgtc
    10621 acaaaagaat tcgtaaaacc aggtgctgta gtgatagatg tagggatgaa tcgtgatgct
    10681 aacggtaagc tgatcggtga tgtgaaattt gatgaagtgg agcctattgc aggctggatc
    10741 actcctgtgc caaagggtgt agggccaatg actatcacga tgctgatgta tcaaaccgta
    10801 aaatctgccg aagcttttgg agcaggtgaa tagatggcac aagaatattt aactgtcact
    10861 acattaacga agtatttgaa aagaaaattt gatgccgatc cttatttaga acgcgtatat
    10921 ttaacaggcg aaatatcgaa tttccgttta cgtgccaatg cccatcaata tttcagctta
    10981 aaagatgacc atgcaaaaat atctgctatt atgttcaaag gagcattcca aaaattacga
    11041 ttccagccaa aagaaggaat gaaagtcctt gtcgttgggc gtatttcatt atatgaagcg
    11101 ggaggttctt atcaaatcta tgtagagcac atggagcctg atggaatagg tgctttatat
    11161 caagaattgg aagaacgaaa agaaaagctg atgaaggaag gcctttttca agggcctaaa
    11221 aaaatgctgc cacgtttccc aaagcgaatc gcggtcctta ccagtccaag cggagcggtt
    11281 atccgcgata tcatcacgac ggtgaaaaga agatatccaa ttgtccaatt ggttttgttc
    11341 ccaacagttg tacaaggaaa ccaagcagcg gacaatgttg tcaggaatat ccagagagta
    11401 gaagagatgg gcaatttcga tacaatgatc atcggtcgtg ggggtggttc gattgaggat
    11461 ttatggccgt ttaatgaaga gcgggttgct cgggcgattt atcaagctaa tacgccggtg
    11521 atttcatctg taggacatga aacagatacc acgatcgctg atttagtagc agatgttcgg
    11581 gctgccactc caacagcagc tgcagaatta gctgttcctg ttttgtcaga agagttgatg
    11641 cgcattcaag aacggcaagc tagattgcag caggcgtttg tacgccagat ccagcgcaaa
    11701 caagaacggt tcgagcgtgc tcgacgttcc tatgtatttc ggcagcctga aagattgtat
    11761 gaaggtcaga ccatcaaatt agaccagttg aaacagcgat tatatcaagc tacacagcaa
    11821 atttatcatg cgaaagaaaa acaaacgatc gcattaagcc accaattgga acaagtggct
    11881 ccgctttatc gagttaaagc agctcgccaa gaaactgctt atctagaaaa acgcttagaa
    11941 gagaaaatcc aacaatatat gaaacaacag cagcagcgtt ttgctcaagc tgttcaatct
    12001 cttgatttgt tgagtccatt gaaaatcatg ggaagaggct acagctatac gacgttaaat
    12061 gatcaagtag taaaaagtgt caccgagatt catcaagggg atttgctgac gatccattat
    12121 caagatggaa tcgttaaagg acaagtacaa gagatagaca aaggagaata aaaatatgga
    12181 aaatccaact tttgaagagt cactgcaaga attagaaaaa attgtcatgc agttagaaca
    12241 aggagacgtt ccattagaat cagctctaga ttcgtttaaa cgaggaatgg aattaagcaa
    12301 acattgccaa gatacattag ctaaagcaga agagaccttg acgaaaatga tgacagaatc
    12361 aaatgaagaa gttgattttg acggaaacga ggaaagctga gatgagatta actgactttt
    12421 cagctattca tctacctgct gtagaaaaag aaatcttgtc ttttttagat gagcatacaa
    12481 cggatagagg actctatgat gcgatgagtt attcagtgaa agcaggcggg aaaagaatac
    12541 gtcccttatt acttttaacg gccgttgctt cctttgatga gcctattgat gttcccgtct
    12601 atcaagtagc agctgcttta gaaatggtgc atacgtattc cttgatccac gatgatttgc
    12661 ctgcgatgga caatgatgat cttcgtagag gaaaacctac caatcataaa gtattcggcg
    12721 aagcattagc tatattggcc ggagatggtt tgcttacagg agcattccag ctaatcagta
    12781 tggcacactt aggtaattct cctaagcttc tgctgcttca gcaattagct gtttgtgcgg
    12841 gatcacaagg tatggttgct gggcaagctg cagatatcga gggcgaatcg aaaaagcttt
    12901 ctttagaaga attagcattt attcatgaaa gaaagacagg gcatttgatt cgttatgcct
    12961 tgctggctgg tggcatatta gcgaagcaac ctgaagaaat cttattgcta cttcaacgat
    13021 tagccgaaca tttaggttta gcttttcaga taagagatga tctactcgat gtgattggta
    13081 caacgaaaac attagggaaa acagctggta aagatgaacg tatggaaaaa aatacttacc
    13141 cgcgtttatt aggcctagaa aagacacgtg aggcgttgga gattgagctg ttatccgcca
    13201 acaagatcat agacaaatta gaagaggacg ttttttcttt tgatggcggg ctgatgcgcc
    13261 agatgatcaa gcaatttgat gtggagaaat gagggaaaga atggaaaaag aacgcgtaga
    13321 tgtattagcg gtcaaacaag gattatttga aacaagagaa caagccaaac gttcagtgat
    13381 ggctggtttg atttacaacg aaaaaaacga gcggtttgat aaaccgggag aaaaaattcc
    13441 agtgagtagt gaattaagag tcaaaggtaa aaaacttcct tatgtttctc gagggggatt
    13501 aaaattagaa aaagcactca aagcatttga tctatctgtt gaaggaaaaa cattgttaga
    13561 tattggtgca tccactggcg gatttactga tgcagctttg caaaatggcg caaaaatgag
    13621 ttatgcttta gacgttggct acaatcagct agcttggaaa ataagacaag atcctagagt
    13681 cgtcgtaatg gaaagagtga attttcgtta tgcaaaaccc gaagacttta ccttaggaat
    13741 tccagaagtt gctgttatcg atgtttcttt tatttcacta aaactgatgc tgcctccatt
    13801 acatgctatt ttaaaagaag agggagaagt gattgcttta atcaaacccc aattcgaagc
    13861 aggcagagaa gctgtaggga aaaacggaat tgttcgtgac cctcagaccc atcaaaaagt
    13921 tttagaagac attttgtcat ttgcggcaca aggtggatat gatgtgctgg aattaagtta
    13981 ttctccgatc acggggggag aaggaaatat cgagttcttg gctcacttga ggaaagtacc
    14041 cgaaagcgga acaatcaatt cagctattaa tatggcagaa gtggtttcaa atgcacacga
    14101 acagtttgac cataaataaa ttgtaactgt gaaaatcaag tgagaagtat gcggattctt
    14161 ctttattata ccaaggaaat aagctatcat tgattaagaa gaatatgcta aggagcgaag
    14221 acgtaatgag aaaaaaagat cggcatcgtt tgattactcg attgttgagt gagcaagata
    14281 tccgaaagca ggaagaattt gttgaaatcc tgaaaagcaa aggaatatct gttactcaag
    14341 ctacgatttc cagagatata aaggaactga aactgatcaa ggtaccggct tcagatggcg
    14401 gctaccgtta tagcatacca gctgaaacaa acgaagatgt aaatactaaa ttggaaaaac
    14461 tattaaaaga cgctttcgtg gcagttgacc aaatggaaaa atttgttatt ttgaaaacat
    14521 tgccgggaaa tgcttcggca gcagcaaatt tgattgataa acgttacaag aaagaattat
    14581 tttcgattat caatgatgac gataatgttt tgatgattac acgaactgaa gaccaggcag
    14641 ttttattgaa gaaagacttc ctgcattacc tttaagttga ggagcgaatg tgatgctaca
    14701 agaaatcagt attactaact ttgcgattat tccggaattg cggctttctt ttcacgaagg
    14761 aatgaccgcg ctgaccggag agactggtgc gggtaaatcc attattatcg atgctttagg
    14821 tctgttagca ggaggaagag gttcaagcga ttatatacgt caaggagcag aaaaatgtgt
    14881 gcttgaaggg ttgtttgaac taccaaaaca agaaggattc tctgaactga tggttgaatt
    14941 agggatcgaa acagatgagg acaatctgat tgttcgacgg gatatgtcct taactggaaa
    15001 aaatgtttgt cgagtgaatg gtcatattat cacattagct aatctgagaa gaatcggcag
    15061 ttatttagta gatatccaag gacaaaacga acatcaggaa ctgctccagc ctgaatcaca
    15121 tcttgcttta ttggatcgtt ttggagatgt agctttccaa cagaagaaaa agagttatca
    15181 gcaagaatat atctcttatc gtgagctgga aaaaagggta cgaaaagtgc agcaaaatga
    15241 aaaatcctat gttcagagaa tagatatgct ccatttccag caagaagaaa tagctgctgc
    15301 tgatttgcaa gtcggagaag aagagaaatt aaaagaagaa agagaaaaac ttagcaatta
    15361 tcaaaaaatt gctgatggat tagctgcagg ttacggagca ctgacgaata atgaacaaaa
    15421 cagtgtagat ggtgttggct tggccgtttc agaactgcaa gggatcgccc atctagatgt
    15481 ggagtatgaa gcaatttatg aaaatatcca aagtgcttac tatttgctgc aagatgcaat
    15541 cggcgatatg agtcgtcaga ttgatttatt ggagttagat gagagtcgtt tagaagaagt
    15601 tacgcaacgt ttagaattaa tccgacagtt gaaacgtaaa tacggcgaat ccattgagtc
    15661 aatattggct tactatgacg aaatcaccga agagctagct tcttctgatt tttcagaagg
    15721 tcaattggac aaaatgaaag aggaactaga gcaaaaagag cttctacttc agcaacaagc
    15781 cgcagatctt catgaagcgc gtaaaaaaat cgcaaaagaa ttggaaaaat ccattctgca
    15841 tgaattgaaa agtctgtata tggaaaacac tgagtttgag gtacgtttct taaaagaaga
    15901 aaatagacaa ttgaatcatg atggatttga ccaaatcgaa ttttacatta cgaccaatcc
    15961 aggagagcca ctgaagcctt tggtgaaagt cgcttctggt ggcgaactgt ctcggatgtt
    16021 gcttgctttg aaaactattt tttcttctga gcaaggtgtg acaagtatta tttttgatga
    16081 agtggataca ggtgtgagcg gaagagttgc acaggccatc gcagataaaa tttctcaaat
    16141 atcgaaatac tctcaagttt tgtgcatcac tcatctgcct caagttgctg cagtcgccga
    16201 ctatcaatat tatattgtga aagcagtgat tgatggacgt acgcaaactt ctgtttcaga
    16261 gttgaaaaca aaagaaagag aagaagaaat tgcacgtatg ctggctggaa gtgagattac
    16321 aaaactcaca ctcgaacatg caaaagaact attgaaactc gcaaaaagga cctgagccac
    16381 ctcgcagtgg ttcaggtcct tttcagcagc tgatagctat attttatttt tacatgacac
    16441 tcattacagc agtcaataca acagatccaa tcgttaaaat cagcattgtc caaatgacaa
    16501 attttgttac tttagcaaaa gtacttgttt gttttttttc acgcattgtt ttcaacctcg
    16561 tcattaatta gttgattcaa gtgtacaatc ttttttccat ttccacaaga gggctttttt
    16621 ataaaaactt cccttttttt aaataatagg tagttacgat acaaataaat aatgttaata
    16681 gaactagcca tataaaggca tcatcccttt cagcaatagg taacttaatg ttcattccat
    16741 agatactgcc gacgattgtc ggtatagtta aaataatcgt taaagaagtt aaaattttca
    16801 taatattatt cagattattc gatacgattg ctgaaaaagt ctgattcatt tgttctacta
    16861 gttttaattg aatgcgagaa gtagtcattg cctgctctgt ttccaccaat acatcacgta
    16921 aatgattttt caaagcatga ttttcttgga aaagtttatt ttgcgctagt tctttcaatg
    16981 catctaaatt agcagaagtc gcttctctga aataaacgag gcttttttgg atatccatga
    17041 tttgataaag ttgtttgttt tcagtggata cttgaagttt ggcttccaaa gtatcgcatt
    17101 gagattgaag cgtagctaaa taactgttgt aagagagtgt gacctgccaa atgaattgaa
    17161 agaagatatt gagattaggc gaaatatcac tttttggaaa ggtctggcta aagactttct
    17221 ttaaaaacag tggttcatga ttcgtaattg tgataagctt ctttttgggc gtcaaaataa
    17281 tagagagcgg gtatgtatcc acttgaaaat aaccgctagg acttgtttta gcaaaaggat
    17341 attgtaacaa taataacgct ggctttgtca actttgattg attgaattct tctgtacggg
    17401 aattttctgc atcgtccaat atggaagtga tgtaatctga cggaatttca aattgatgtg
    17461 tcagctgatg aatctcttct tgattaggac gttctacaca ataccagatc gtttggtcag
    17521 tttcctttcc ggtattacgc agtttctcat tatctaatgt aaaatatttg atcatgattt
    17581 tcctcctgtt gcctgtttct tttattataa cggataggga attggaatac atggaatgtg
    17641 tttgtaagat atagaatata actgcataaa tttaaataaa atgaaacagt tgtgtaaagt
    17701 atatacagta tacttgaagc aacgataaaa aaggagttta cgttcatggg cacattcaca
    17761 ccacttatct tgttttggta tatttacccg gtaatcgttt tgtttggttg ccaatttctt
    17821 gtaaaattat tttcgttaaa tcgtcgtttt aaaataaaag cccctgattt ggcgattcct
    17881 tttttgtggg gcggtatgca tgcattgtct agaaatacag ggacaatttc tatcttaccg
    17941 tttttcttta tttctgtttt gctaatggga atttgtattg ctgttttcca agcatattat
    18001 tatgaagaaa ttatttatcc gcgttatttc aagatgactt ggcgtttggt ttttctgttg
    18061 acaatcgtct tgtacgctgt cttgatcatt gtgaacatac tttcttacct atgaaaaaaa
    18121 tagaaaagct gtaaaaagtc ttttaaaagg ctttttgcag cttgattttt aaaaaaatgt
    18181 ttatgaataa aaaaagttgt tcccggattt cccagaaaag gtggtagaaa gtgggggatt
    18241 gtggtagact aattttagtt agtggaggaa tggggggctt tcaatgttga tgggtgaatt
    18301 ccaacataat attgatgcaa aaggacgttt aatcgtgccc tcaaaacttc gtgaagaact
    18361 tggcgaaaag tttgttctga cacgcggatt ggatggctgt cttttcggct atccgatgag
    18421 cgaatgggaa aatctggaag ctaaactgaa cgaaatgccg ttggctaaaa aagacgcacg
    18481 tacttttgtg cgattcttct attcggctgc cactgaatgc gaacttgata aacaaggacg
    18541 aatcaatata cccagcacgt taagaaatta tgcagctttg acaaaaggtt gcgtagtcat
    18601 cggtgtatct aaccgaattg aaatttggga tgagacacga tggcaagatt tttctgcagc
    18661 agcagaagag aactttgacg aaattgctga aagtatgatt gatttcggac tataagaata
    18721 ggaagaggct gactaatgac tgaaacattt caacactata cagtcatgtt gaaagaaacg
    18781 gtagatggat tgaacatcaa accggatggc gtttatgtag actgtacact cggcggtgcc
    18841 ggacatagtg aatatttatt aagccaacta agttctaaag ggcatctgta tgcgtttgac
    18901 caagatcaaa aagcaatcga tcacgccaag ctgcgtttag cttcttatgt cgaacaaggt
    18961 atggtcacat ttatcaaagc gaacttcaga gaattagaag aacgcttgag tgagcatgta
    19021 gataaggtag acggcatttt gtatgatcta ggggtttcct ctcctcagct agatgaagca
    19081 gaacgtggat ttagttatca tcaagatgca ccgttggata tgcgcatgga tcagtcagct
    19141 cctttatccg cttatcacgt aattaatgaa tattcctacc atgaattagt aaagattttc
    19201 ttccgctatg gcgaggagaa attttccaaa caaattgctc gtgaaatcga acgagtacga
    19261 aaaacaaaac caatccaaac aactggtgag cttgtcgaaa tcattaaaca agtcatccca
    19321 gctcccgcaa gaagaaaagg cgggcatcct gcaaaaagaa tttttcaagc tgtgcggatt
    19381 gcagtaaatg acgaactcgg tgccgaagag gcctctttag aacaggcaat ccggttattg
    19441 aaagtcaatg gaagaatcag cgtaatcaca ttccattcat tggaagacag aattgtcaaa
    19501 agtatgttta aagaatatag tacggtacaa gatctaccac cgggacttcc tgtcatacct
    19561 gacgaattcc aaccagaact gaaagtcatt actcgtaaac caatccttcc ttcagaggaa
    19621 gagctaacag aaaacaatcg atcacgcagt gcaaagttac gtattgcaga aaaaataaga
    19681 atgaaagagg agtagaatga tggcagaatt aaagaaaatg caagattacg aatatgattt
    19741 acctgaattg gtggatcagc cggaacaaca aatgcctgat tcttcttcga atcaagaaat
    19801 cattcttcca caatcaccgg caaaacgatt aaagcagatt tctgctttgg aaaaaattgc
    19861 tgtggcaagc gtggcgattg ctattgttgc tttatgtgtt ttaacagtta tgctgcgcac
    19921 aaatatcagc ggagctgaaa aagaaatcac gtcaatacaa acagaaatca atgataaaaa
    19981 gcaagaaaaa acgagtttag aacaagaaaa aaacgaactt tctcgtactg aacggattaa
    20041 acaaatcgca gaagaaaaag gattgtctat taatgacgac aatttaagga aagtgaagta
    20101 aatgagtctt aaaaataaaa ttcggcggtt tatggaaaag aaaaatctaa atccgatgaa
    20161 caatcgcaaa aaagtaggta tcattttatt tgctacgagt attggattgt tctttttatt
    20221 tgccgtccgt ttctcgtata tcgttattgg aggtcatgtt gctggtacct cattagctga
    20281 aaaaacaaaa cagttgtacc aaggaagtga agtagtaaaa gcaaaaagag gaacgattta
    20341 tgatcgtaac ggtgtggcgc tcgcagaaga tgcctcctct tattcaatta aagcgatact
    20401 ctcaaaaaca tatacctctg gggataaaaa actgtatgtt gaagaaaaga attttgataa
    20461 aatagcagaa atcctgcata agaatctttc tatagacaaa aaagatgcac tgaatatttt
    20521 agaggacggc gcaaaaaaag agctgtatca agttgaattt ggtagctatg ggaaaaatat
    20581 ctcccaagaa accaagcaaa acatcgaagc ggacatgaaa aaagaaggtg tcgctggtct
    20641 ttattttgtc gaccatcagg ctcggatgta tccaaatggc gtcttttctt ctcattttat
    20701 tggatacgca gttccagaca aagatgagaa tggcttagtt ggtaaactag gtttggagtc
    20761 tgcttacaat gatatcttga gcggtaaaga tggtaagatt atttatcaaa aagataattt
    20821 tcaaaatcct ctgccaggta cagtggcaga agaagaaaaa gctgtcgacg gacaagatat
    20881 ctacacgact ttagacagca gactacaaag ttatttagaa acactgatgg atcaagtaaa
    20941 tgaagaatat cagccagaag aattgacagc agtcctgatg aaagcaaaaa ccggagaaat
    21001 cttggcaatg ggacaacgac ctactttcaa tcctgaaaca atggaaggat tgactggaaa
    21061 agatgctatc tggcgtaatt tcttggtaca agatagttat gaaccaggat caaccatgaa
    21121 agttttcact actgcagctg ctatcgaaga aggagaattc aacgagaatg aaaccttcca
    21181 atctggaaaa atccaagtag aagatgctac gatcaatgac catgactttg gtgaaaaagg
    21241 tgtgctgacc atgcgtcaag ctctttcatg gtcaagtaac gtcgggatgg tcatactgga
    21301 acagcgatta ggcggacgat ggtataacta tttacaaaaa ctgggatttg gacaaagtac
    21361 tcattccgga ttagatgatg aagtaaacgg tgctttacct acattaaata ttgtcgaccg
    21421 agcaatgagt gcttatggac aagcggtagg tgtgacgaat ttccaaatga tgaaagcttt
    21481 cacttctatt gcgaataacg gtacaatgat tcaacctcga tatatcagca aagtggtgga
    21541 tccccaaact ggagaagagc gaacaactca aacagaagtt ctagggcaac ctttttcaaa
    21601 agaaacaaca gaaaaagtcc gtgaatatat gagagatgtt gtcgaaagcg aaaactacgg
    21661 tagtgcttat ggtgttgtac ctggatacaa tgtatcggca aaaaccggaa cagcccaaat
    21721 cgcttctgat acagggggat atcaaacagg agatacagcc tacctgtatt cgattgtgga
    21781 gatggttcct tctgaagatc cagactatgt cttgtatcta acaatgaaac accctaagac
    21841 atatgatcga atggcattgg ctaaaatcgc aaatccattg atgaaacggg caatggattt
    21901 taaagaaact gaagaagatt cagatacaga aacaaaaaca gaaaaagtat ccgtagccga
    21961 ttatcgtaat cttgaagcgg acgttgcagc agctgatgct caaaaaagtg gactgcaacc
    22021 tgtagtgatc ggtaatggta aaaaagtaca aaaacaatct actgccaacg gtgatcagtt
    22081 gatctctgga gaaaaactga tcctatatac tggtggtgat aagctgatgc ctgacgtaac
    22141 aggatggtca aaagcagata tcatgaagct ggggaaaatc ctcggtattg aagttagttt
    22201 tgatggagat ggttattgtg tcaaacaaga acttgctcca tatgaaaaaa tcactgaaga
    22261 caaacttagt tttactttag aagaataaga agagaaatag gagagaaaag agcatggaat
    22321 ggacacaagc attgattccc attgtcagca gttgtgcaat gacaattgcc gcaatgccgc
    22381 tatttattgg ttattttcaa atgaaaaagc aaggtcaggc gattcgtgag gagggaccaa
    22441 agtggcacaa tagcaaagcc ggaactccta caatgggcgg cttagtgttt ctgatcggct
    22501 ctattttgac aggaatttgg gttggagctt ggcaaaaaca gctaactcct actcttttca
    22561 tcttgctttt tgtattagcg ttatacggag taatcggctt tcttgatgat tttataaaaa
    22621 tattcaagaa aagaaacatg ggactgaact cgaagcagaa attgattgga cagattctag
    22681 ggggaatcat cttttatctt gtttaccgtt ctgaaggtta tccaggtgta ttgaatttct
    22741 tcggactaga tctaccttta ggaatcattt atggtatttt cgctctcttc tggcttgttg
    22801 gattttcaaa tgcagtaaat ctgacagatg gcatcgatgg attagttgct ggcttaggaa
    22861 gtatctcttt tgcagcttac ggtctaattg cttggcatca gcatcagtat gatgtattaa
    22921 tcatttgctt aagtgtttta ggaggtttac tgggtttctt cgtctacaac agaaaacctg
    22981 ccaagatttt tatgggagat gtcggttcat tagcgttggg cggactttta gcggcaattt
    23041 ctataatgct aaaccaagaa tggacattgc ttttgattgg tttgatctat gttatggaaa
    23101 cagcaagtgt gatgcttcaa gttacttcct ttaagcttac aggaaaacga atttttaaaa
    23161 tgtctccaat tcatcatcac tttgaaatgt gcggatggtc ggagtggaag attgatacag
    23221 ttttttggtt gatcagcatt gtgacttcgt tgatcacatt atggatcgtt tggtaggaga
    23281 tgacgggaaa tgaaaaaaat atcaacatat gaaaataaga aagtgcttgt cttaggactc
    23341 gcaaaaagcg gagtaagtgc ggccaaatta ttacatgaat tgggcgcact tgtcaccgtt
    23401 aatgatggaa aaccttttga cgaaaatccg gaagcacaag agctgctttc tttagggata
    23461 aaagtaataa caggtagtca tccaatcgaa ttgttggatg aagaattttc attgatggtc
    23521 aaaaatccag ggattcccta cagtcatcct ttagtagcaa aagcccaaga aatgggtatt
    23581 ccggtaatta cggaagttga gctagcttac gaagtatcgg aatgcccaat tattgggatt
    23641 acaggaacga acggaaaaac aacgacaaca acgatgacag gactactact gaatgcagga
    23701 gcagatcaag gaattgcacg tttagcgggt aatattgggt atccagcaag tggtgtagcg
    23761 caagaagcaa aatcagaaga taaaattgtg atggaattgt ctagcttcca actcatggga
    23821 atcacagatt tccgccctca tatagcggtg attaccaata tctatgaagc acatattgat
    23881 tatcatggta caagaaaaga atatgtcaaa gctaaatgga accttcaaaa aaacatgaca
    23941 gaaaaagatt atctgatcct taactggaat cagtcagaac tacaagaatt agcgcaaaga
    24001 acaaaagcac gcgtactgcc tttctcgaca aaagaagtgt tggaagacgg tgtgtatgct
    24061 gacgattact caatatacta taaaaaagaa aaaataatgg aaatatctga acttggcgta
    24121 ccaggtaagc ataatgtaga aaatgcacta gctgctattt ctgtagcaaa attgtatggt
    24181 atatcaaatg aagctatacg agaaacgttg cattttttcc atggcgtacc acatcgtacg
    24241 caatatgtag gagaaatcca aggcagaaaa ttttataatg attcaaaagc aaccaatatt
    24301 ttagcgacta aaatggctct aagtggattt gaaacgtcta aggtcgtgtt attggctggc
    24361 ggattagaca gaggaaatac atttgatgaa ttaattcctt cattaaaagg aatcaaagca
    24421 atggtcgtat ttggccaaac aaaagaaaag ttgatggatg ctggaaaaaa agcaggcatc
    24481 gagacaatag taacagccga ttctgtagaa caagcagttc ctttagcatt agaaaacagt
    24541 acagatggtg atgtagtttt attatcacca gcgaatgcca gctgggatca atacccaaat
    24601 tttgaaacta gaggaaacag attcatggaa gctgtgaacc gattaaaata gaaaaagtag
    24661 aagcgagcga taaaaaatga aaattttggt tacaggcgga ggaacagggg gacatattta
    24721 tccagctctt gcctttgtca attatgtaaa gactcaagaa ccgaatgctg aattcatgta
    24781 tgtgggagca aaacgaggtc tagaaaacaa aatcgtccct gatacaggaa tgcctttcca
    24841 taccttggag attcaaggat ttaaacgaaa aatttccatg cataacatca agacggtccg
    24901 attgtttctt aagagtatcc gtcaagcaaa aaaaatcctc agtgattttc aaccagatat
    24961 cgttatcggt actggaggat atgtttccgg agcggtagtg tacgctgcag ccaaaatggg
    25021 tattccaaca gttatccatg aacaaaacag tgtaccaggt gttaccaata aatttttaag
    25081 ccgatatgtg gatcggatcg ctctttcatt taaagatgcg gcttcctttt ttcctgaaaa
    25141 caaagcagtt cttgtaggta atcctcgagc acaagaagta gcagatacta aaaaatcaga
    25201 tattttgaaa acatttgatt tggatccaga aaagaaaaca gtcttgattt ttggcggtag
    25261 tcaaggagca cttaaaatca atcaagcagt atcagttttt ttaagtgaat atattccttc
    25321 ttcttaccaa gttttgtatg cttcaggaga tagatattat gaagagatcc taaataaaat
    25381 aaatcctgaa atacaaaata ttagtgtcca gccttatatc aaaaatatgg cagaggtcat
    25441 ggcttccagt gatctgctgg tggggcgtgc tggtgcaact tcgatcgcag aattcacagc
    25501 tctggggtta ccggcagtgt tggttcccag cccatatgtt acgaatgatc atcagactaa
    25561 aaatgcaatg agtcttgttc acgcaggagc agcaaaaatg attgcagata atgaactaac
    25621 aggtgaatca ctaagtcaga cagtcaacga aatcatgggt gatgaagaat tgcaaaagca
    25681 aatgtgccga gcttctaaag aacaaggaat ccctgatgct tcaaaacgtc tttatgactt
    25741 agtaaaacaa ataatcaaaa aataaaggcg gtggtcttag tgagtaagaa aaaagaacca
    25801 aaaaaattgt ctcatccgtc ttctgatgaa aacaatttga ctccttggca aaaggctaac
    25861 cgaaaatatc tggcagaaca agagatgaaa aaccaagagc caaaaaaaga agacataaca
    25921 ttagatacac ccctttcaga ttctgaagaa gaaaaaacgg ttcaagagga acagcaagaa
    25981 gaaagccagt ccgagaaaaa aatcgaattt tttgaagaga tcaagcaaaa gaaaaacggt
    26041 ggaccatata acggttcttt tttgaaccgg ctgcccaatt taaaaagtca acggcacaaa
    26101 gtactatttc gtcggttagc tttgatcatc actgtattgg gtataccgct gatttttttg
    26161 atctattatg tttctcctta tagcaaatta caagcagttg cagtatcagg caataagaca
    26221 gtcaacagcc aagaaatcat ctctgataca aaattgtcat taggagaaaa tgtttgggga
    26281 cagtattttc atcgatctac ttatatcgat cgccttaaaa aagcacagcc aagaatcgaa
    26341 acagcaaata tacatttcaa agggatgaat gaattcgatt tggatgtaac agaatataaa
    26401 gagattgctt tgatcgcaaa aaacaatcaa tactacccag tgattgaaaa tggaacagta
    26461 ttagatgaaa aagtagcaaa tcctacaaaa aatctcccta tcttggaaga gttcaaagac
    26521 tctgcaaaaa tcaaagaatt aacaaagcaa tacaatcagt tatcatcaga attacaaaaa
    26581 gcaatttctg aaatcaagta cacgccaaga gcaagcaata aaaatctgat ccaattgaat
    26641 atgaatgacg gcaatcaagt tattgtaaat atcaataatc ttgcgaacca aatgaaatat
    26701 tattcgcaag tagccaaaga tatggatgaa aaaggtgtga tcgacatgga agttggtatc
    26761 ttctcgcatc cttacggtag cactcaagaa acggaagaat cgggccaatc ggaagaaaca
    26821 actgaaagtg aagaaaatgc ggatcaaaca aacgaatcaa gtgaagaggc agaaacgcaa
    26881 aaagcacaaa cacctggatc gtctagcaca aacggatcat ctgattcaaa tgattcaagt
    26941 gaaacaagtc aaatagaaga taatccaagt acaactccgt caagtttaaa tgaaaattga
    27001 taaaacaact ttaaaaagta agaatgtact ttctaaaaac cagtaattta tggtagaatt
    27061 ggaagcagtg aatatatcta ttaataagta tattttaaaa cggacgctag ttgttcgctt
    27121 gtatatggaa tagtaagtag gagggaatcc cattcatggc aaaaacagga atgtatgtag
    27181 gccttgatat tggaacgaca tctgttaaag ttgtcgtggc tgaatatatc gacagccaaa
    27241 tgaatattat tggagtagga aacgcaaaat cagaagggat taaccgaggc attatcgttg
    27301 atatcgataa aacggtccaa gcgatacaac gagcagtacg acaagcagaa gaaaaagcag
    27361 gtatccagat caaaggcgta agtgtcggtt tgccagcaaa catgcttgaa gtagaaaatt
    27421 gccagggaat gattgcagtt aacggagatt caaaggaaat caccgatgaa gatgtcagaa
    27481 atgtggcttc agctgccctt gttcgttcaa tacctcctga acgtcaaatc gtgtcaatat
    27541 tgccacaaga ttttactgtc gatgggttcg agggaatcaa agatcctcgt ggaatgatcg
    27601 gcgtaagatt agaaatgtac ggtctgttgt ttacaggacc taaaacgatc atccataaca
    27661 tacgtaaatg cgtggaaaat gccggattaa tcgtcaacga aatggtgatt actcctttag
    27721 cgctgactga atcaattctt tctgacggag aaaaagattt tggtacgatc gtcattgata
    27781 tgggtggcgg ccaaacaaca acggctgtta tgcatgataa gcaattgaag tttaccaatc
    27841 ttgaccaaga aggtggcgaa tttgtcacta aagatatctc tattgtatta aatacgtcat
    27901 ttaacaatgc agaagcgttg aaaataaact acggggatgc atacccagaa cggacatcac
    27961 cagatgaaga atttccagta gatgtgattg gtcagtcaga accagtgaaa gtcgatgaac
    28021 gctacttgtc agaaattatt tcagctcgaa tggaacagat tttcaacaaa gcaaaagatg
    28081 cattggacca aatcgaagca ttagagttgc caggtggcgt cgtattgaca ggcggagctg
    28141 ctagtcttcc tggtgttgtg gacttggcac aagaaatctt cggcgtaaat gtaaaattgt
    28201 atgttccaaa tcacatgggg ctgagaaacc cggtatttac aaatgtaata agtatcgtgg
    28261 attattcagc aaacttgagt gaagtgtatc aattggcaaa aattgctgtt acgggtgaaa
    28321 cctcagcagc tcgtcaaatg gtagttgaac aagaaacaac aaacacatat gagagctatg
    28381 aagcacctga agaaacaatc tacgatgagc cggaaccaaa agagtccgga gaaaatgtaa
    28441 aaaataaaat caagggtttc tttacaaata tttttgatta attcatcctg ggaggaataa
    28501 gatatggaat tctcaattga taataatatc aatgacggcg cagtaatcaa agtcatcgga
    28561 gtcggtggtg gcggtggaaa tgctgtaaac cgaatgattg aagaaaacgt taaaggtgtt
    28621 gaattcatta cagccaacac agacgtgcaa gcattaaaaa attcaaaagc tgaaacagtt
    28681 atccaattag gccccaaata tactcgtggt ttaggtgctg gttctcagcc agaagttgga
    28741 caaaaagcgg cagaagaaag tgaacaatcc ttacgcgaag ctttagacgg tgcagatatg
    28801 attttcatca ctgcaggaat gggtggagga accggtacag gtgctgcacc aatcgttgca
    28861 ggtatcgcga aagaattggg cgcattgact gttggtgtcg tgacacgtcc attcactttt
    28921 gaaggaccga aacgtggccg ttttgctgca gaaggtatcg ctcgtttgaa agaaaatgtg
    28981 gatacattat taattatctc aaataaccgt ttattagaag ttgtagataa aaagacaccg
    29041 atgcttgaag ctttccgtga agcagataat gttttacgac aaggtgtaca aggtatctct
    29101 gatttgatca cagcgccagg ttacgttaac cttgactttg ctgacgtgaa aacagtgatg
    29161 gaaaaccaag gtacagcatt gatggggatc ggtgtggcta gtggagaaga tcgcgtagta
    29221 gaagcaacga aaaaagcaat ctcctctcca ttgcttgaaa catctattga tggagctgaa
    29281 caagtacttc ttaacatcac aggcggtttg gatatgacat tgttcgaagc acaagatgct
    29341 tcagatatcg tagcaaatgc agcaactggc gatgtaaata tcattctagg tacatctatc
    29401 aatgaagaaa tgggcgatga aatccgtgtg acggtcatcg caacaggtat cgatgaatca
    29461 aaaaaggaac gcaagtctag ccgaccagct agacaagcac aaatgcaatc acctgctcaa
    29521 aagactgtat tagatatgga tcaagctaaa ccaacttctt cagaagaaga aaacagtttt
    29581 ggcgattggg atatcagacg tgaacaaaat gttcgtccta gagttgatga ttccaatttt
    29641 gacaatatcg aaaaaaaaga atttgataca ttcaaccgcg aagaaacaaa atctcatggc
    29701 gatgatgaat taaatacgcc accgtttttc cgtcgtaaaa aataaaggag aggaagagca
    29761 catgattgct gacaacttgc agaaaatcaa gcaagaaatt caggattcgt gtgctcttgt
    29821 tcatcgtgat ccgaaagaag ttacattagt agctgtaaca aaatctgttg aatccgctca
    29881 agctgaagaa ttggttagag ctggcgttat gaattgtgca gaaaaccgag tagataaatt
    29941 attagaaaag aaacaagatt tatttgattt tcctgaaata aagtggcatt taattggtaa
    30001 tttacagcgc agaaaggtaa aattgattgt aaatgaaatt gattactttc atgcgttgga
    30061 cagtttaaga ttagcagaag aaatccagaa gcggttagag aaagaattat cctgttttat
    30121 tgaagtaaat gtttctggtg aggaatcaaa acatggaatt tcaccagatg agcttttacc
    30181 gtttgtggaa tctttagctg cctgcacaaa aatcaaggtt gttgggttga tgactatggc
    30241 gcctaaagaa gcttctgatg agcaaatcca taaagtgttt ggtacgctga aagaattgcg
    30301 tgatcttgtt caagcaaaag gttatgccca tgcaccatgt agtgaattaa gtatggggat
    30361 gagtcaagac tttcaaattg cgattgaaga aggtgcaaca tttatccgtg taggaacagc
    30421 gctattcaaa gacgaataaa ggaggaacaa aaatgccgct tttaaacaga ggagcaattg
    30481 ctaatttttt cggattagct gatgaggaag agtacgaata ccaagattat cctgaaacaa
    30541 caaaaaaggc tgtacagcca actaaaaaaa atacaacaca gaatcaatca actcattctg
    30601 tatctgctgc gaatactcat gaaaaacaaa aaacggcgac ttcacgtcct gtaacgcatg
    30661 aaagtattca gccggctgaa aagaaaaaag taattgaact acatcaatca cctacaaaaa
    30721 ctgtagcgaa agaaagaaca gcttcaagaa cctctacaca acctaaagca aatcttgctg
    30781 gaaaaatcac tgtaatggaa ccaagagctt attctgaagc tatggcgatt gctaaacgaa
    30841 tcattgctgg agaagcaacc ttagtgaatt ttcatttagt agaagaaaaa caagcgagaa
    30901 gaatcgttga ttttctaaca ggaacagttt atgcactaga tggagatatc aaacgtgttg
    30961 gagatgagat ttttctttgt acaccgactg ggatggagat tgacagttca accgctcaat
    31021 cttttgcaga tgcgaatttg tttgatctgt aagaggagga actacgattt atactattgc
    31081 actattactt catttacttc aacgggcagc ctatttctat agcattatct tgatggtata
    31141 tgcattgctc tcttggtttc caggcgggta tcaatcaggg ctaggacgat tattagctaa
    31201 gatctgcgaa ccgtatatca gtttgttcga tcgtctgcct ttgaggattg gacccttgga
    31261 tttcacaatt ttaggggcaa tcctgatctt gaacttagcc gtacaaggtt tagcacagat
    31321 cgttatatgg ataactgtcc atttagttta ttgattgaag caacaaaatc tttattctca
    31381 gaatagatag gaggggaaaa ttttggatgc aaatgtatac cagcactttc gggcagatga
    31441 acgtacattt atcgattcgg taggcgattg gattgaacag gttcaaggac aatatgcacc
    31501 ctatctgacc gaatttcttg acccaagaca atcctatatt ttagaaacac tgattcgaca
    31561 aagcagtgat ttgcgtttca tgttttatgg cggatatgaa caggcagaaa gaaaaagatg
    31621 tttgattttc cccgattatt acgaacctgt agaggaagat tttgaaacag cattgtatga
    31681 aatccattat cctagcaaat ttgccagcct aagtcatggg aaaatccttg gaactttagt
    31741 gggaacgggg atcaaacgct cttactttgg cgatatcatc tcggacggtg atcgctggca
    31801 agtatttatt gcaaatgagg tggatcattt tgttgctgca caagtaacaa agattggaaa
    31861 ggttgctgta cgcttggaac caatcaaata cacagaaatg attatgccga aagatagctg
    31921 gacacaagaa agaggaacag ttagttccat gcgactagac agtgtgatct ctgagattta
    31981 taatatttcc agacagcggt ccaagcagct gatcgaagct ggtaaagtaa aagtgaactg
    32041 gacagaaaat acaagacctg attttgtact tgaactatta gatattattt cagtcagagg
    32101 ttatggcaga gtccagatcc aagaacttga ggggaagacg aaaaaagaaa agtacagggt
    32161 actgttcggt gtattacgta aataaaataa aaaggtggtt tactcatggc tttaactcca
    32221 ttagatatcc aaaataagac attcccgaca aaaatgcggg gatataatca agacgaagta
    32281 gatgatttct tagatttagt tgttcgtgac tatgaggaat tgactcaaag aaatcgagaa
    32341 ttagaaaaag cagtcaaaca ttctgaagaa aaacttgagt acttcaatga attaaaagat
    32401 gctttgaacc aatcaatcat cgtagctcaa gatacggctg ataaagtaaa aacaagtgca
    32461 agcaaggaat ccgaagtgat cgttacttct gcacaaaaca aagcagatga gttagttgcg
    32521 aacgctgaaa aacgtgcaca tcaattaaca actgatgcag aagaaaaagc aagaaaaatc
    32581 ttgacagatg caacagaaaa agcaagacag cttgcgacag aaactgaaga tcttaagaaa
    32641 aaaacacgag tattccatca acgtatcagc ttgatgcttg agtctcaatt agaacaagtg
    32701 aaaagtccgg aatgggatga aattttacaa ccattctcca gctacgtgac tgacagccat
    32761 gaagtgatca aagaagtttt agcaaaacag cttgacaatg aaaatgatac tgatgtaaac
    32821 tcagacacag gagtcataga agcacctatg acggcatttg atacacaggc tattgatatc
    32881 tcgatcgtgc ctggtgaaaa tgaaaacgag tagaacagaa aagcactttg ttattcttaa
    32941 agcgagccga tgagagtgga agttcggtaa gacactgcat ggtgaaagat cctctacttg
    33001 agacctgctt ttccgaaatt agagtaagaa gcagcggctg acctcgttat tggtcacaga
    33061 gagtttgcac tagatagatg caactaaatt tgggtggtac cacggcatgt tcgtcccttg
    33121 cggctgaaca tgcttttttg tgtccggaaa acaaataaag gagtgtatgg ttgaatgaaa
    33181 atgaaagaaa ccttgcaatt aggaaaaaca aaattcccta tgcgtggtaa tttgccaaac
    33241 cgagaaggcg agtggcaaaa agaatgggaa gaaaatcgaa tctaccaaaa aagacaagag
    33301 ttgaacgaag gaaaacctag ctttgttctt catgatggac ctccatatgc caatggtaat
    33361 atccatttag gacattcatt gaataaaatc agtaaagaca ttatcattcg ttcaaaatca
    33421 atgtctggct atcgtgcacc atatgttcca ggatgggata cacatggatt gccgattgaa
    33481 caagtattgg ctaataaagg cgtgaaacgt aaagaaatgt ccatggcaga atacttggaa
    33541 aaatgtcgtg aatatgcact ttcccaagta gataagcaac gagcagattt caaacgccta
    33601 ggagttgcag gagattggga acatccttat gttacattag atccttctta tgaagctgca
    33661 caagtacgcg tatttggtaa aatggctgaa aaaagctata tttacaaagg attgaaacca
    33721 atttactggt ctccgtcaag tgagtcttct ttagcggaag ctgaaattga atataaagat
    33781 gtaaaatctc catctatttt tgtagctttc aatgtcaaag acggcaaaga tattcttgat
    33841 gaagatacag cttttgtcat ttggacgaca acaccatgga cattgccagc aaaccaaggt
    33901 attgctgtaa atccaaaatt tacgtatgta ttggtggaag ccgatggccg taagtttgtc
    33961 gtagcgaaag atttgctaga aacagtccaa tcagaaatcg gttgggaaaa tgtaacagtt
    34021 ctaaaagaat ttgctggaca agaaatggaa tatatgactg caagtcatcc attctatgaa
    34081 cgtgaatcac ttgtgatttt aggcgatcat gttactttag acgctggtac tggattagtc
    34141 catactgctc caggacatgg ggaagatgac tatatcgctg gtagcaaata taaactatct
    34201 gtagtgtcac ctgtagactc aaaaggattg tttacagaag aagcacctgg atttgaaggt
    34261 atcttttatg acaaagcaaa tcctatgatc actgatctat taaaagaaaa aggagctttg
    34321 ctgaagctag atttctttac tcatagttat cctcacgatt ggcggacgaa aaagcctgtg
    34381 atctatcggg caactccaca atggtttgca tctatcgata aattccgtca gaatattttg
    34441 gatgaagtag aaaaagtaga gtgggttatc ccatggggta aaacacgttt gtataatatg
    34501 atccgtgacc gcggcgattg ggtaatttcc cgtcagcgtg catggggagt accacttccg
    34561 attttctatg ctgaaaatgg agaagcaatc atcacaccag aaacaatcga acatgtggca
    34621 aaattatttg ctgaacatgg atcaattatc tggtttgaaa aagaagcgaa agaacttctt
    34681 cctgaaggat ttacccaccc aggttctcct aatggcgaat ttacaaaaga aaaagatatc
    34741 atggatgtct ggtttgattc tggttcttct catgaagctg ttttacgtca gcgaccagaa
    34801 ttatcattcc cagcagatat gtatttagaa ggatcagacc aatatcgtgg ctggttcaac
    34861 tcaagtatca cgactagtgt ggctatcaat ggtgtagcac catacaaatc agttatttct
    34921 caaggatttg ctttagatgg tgaaggccgt aagatgagta aatctttagg aaacatcatt
    34981 gcaccagaaa aagtgatcaa acaatttggt gcagatatcc ttcgcttatg ggtatctagt
    35041 gtagattatg aagcagatgt tcgtgtttct atggatatct taagtcaagt ggcagaagta
    35101 tatcgtaaaa tcagaaatac gatgcgtttc ttattagcga atacagaaga tttcgatcct
    35161 aaaaaagata ctgtttcgta caatgattta cgttctgtag acaaatatat gaccgttcga
    35221 ttgaaccaag taatcaaaga aatccgagaa gaagggtacg acaaatataa cttcatgcat
    35281 atttaccgta cagttatgaa cttcttgacg gttgatctat cgtcattcta cctggatttt
    35341 gctaaagatg tagtttatat tgaagcagaa gatgattatc aacgtcgttg catgcaaact
    35401 gttttctatc aaacagctgt agctttgaca aaattattga ctcctatcat tccgcataca
    35461 gcagaagaaa tctggtcatt cctgaaagaa gaagaggagt acgttcagtt aagcgagttt
    35521 cctggttatg aagagtttgc aaaccaagaa gaattgatgg atacctggac tgctttcatg
    35581 gacttccgtg ataatgtctt aaaagcattg gaagaagcaa gaaacagcaa attgattggt
    35641 aaatctttgg aagcaaaact aactgtttat ccaaaacaac aagttcgtga aatgttaaaa
    35701 gctttggatg cagatattgc acaattattg atcgtttcac cagactactt tgaagttatg
    35761 gcagcagatg aacaagtgcc agataacgct atggtatttg atgatgttgc tattacagtg
    35821 gaaaaagcag acggggaaac ttgcgatcgc tgtcgccaag ttcgtaaaga tgtaggagta
    35881 gatgaaaaac ttcctcatct atgcggtcgt tgtgcaaaaa tcgttgaaga cttctatcct
    35941 gaagctgtag ctgaaggatt cgaagaaaag taaaagggaa aagggggtgt gacatgttct
    36001 ttgtcacacc cccttttgtt atcaatcgtt taattttcca cgttcacgat accagttatc
    36061 tggttgccag atccattcga atccgtcttt ttcaagtagt tcgaaagctg ctttaggtcc
    36121 cattgatcca gctttgtagt tgggaaaatc cggttgtgtt tggtcccaag cttttcgaat
    36181 gacatccacg atatgccaag attgagcgac ttcttcccaa tgagagaagt tcgttccatc
    36241 accattcaac gcatctaata gtaatttttc gtaagcttct gggctatttt caaccatttc
    36301 tgcactattt ctaaattcca atttgattgg ttccgtttca aatccttgcc ctacttcttt
    36361 cccattcaag gataaagaaa atccttcagt tggctgaata tagatcgtta gaacattagg
    36421 tggaagtgtc gtatcgtcac atggttcatc gacagatgtt ttgaatacat taacaggaac
    36481 ttgtttgaat acgatgttga tccgagtccc tttttcagtc aaacgtttgc ctgtacgtac
    36541 atagaaagga acacctgacc aacggaagtt atcgatagtg aatttgcctg cgacgaatgt
    36601 ttcagtctct gattgagcat ccactttgtc ttcttcgcga tagcccttga attgtacacc
    36661 atccaattca ccttgagcat attgcccgcg aacgaagttt tctaacactt cttcttcgtt
    36721 ataaatgcgg atagctttca atgctttgat cttttctgta cggatttctt tttcggaaaa
    36781 tgctactggc ggttccattg caagtaatgc tacaacctgt aaaatatgat tttgcaccat
    36841 gtctttaagt gcgccactat gatcgtagta gcccccgcga tcttctacac cgaggttttc
    36901 gccaaaagta atttggatat tgtcgatata gcgattgttc cattgagcct caaaaatatt
    36961 attggcaaaa cggatagctg agatgttttg gatcatttct tttcctaaat aatgatcaat
    37021 cctgaaaata tcttgttctg ggaatacttc tctgatttgc tggttcaatt caaaagcaga
    37081 ctggtaatca gaaccgaacg gtttttctat gatcaaacga ttgaagccat tttttgtcat
    37141 aatattttgt gatttcaaat gagaaacgat cgttccaaaa aattgaggtg ccatagccaa
    37201 ataatagaca cggttaccgt ttaattgata gtgtccatct aatcggtcag acaattcttt
    37261 taatgtctga taatgcgttg aatcatttac atcatgggat tgatagtaaa aatggcttgc
    37321 aaaggtagtt gcttcttcat ctgaaggatt caatgcacgg attgtttctt tgacaacttc
    37381 acggtagtgc tcatcgctcc agggacggcg agcggttccg attacagcaa aatgctcgcc
    37441 taaatttcct tttctgtaaa ggcggaacaa agacgggtac aatttacgct gggctaggtc
    37501 acctgtaccg ccgaatatcg taaacaatac atttttttca ttagacatgt ggatacactc
    37561 ctttatattt tatcgacaaa aatcgtgctg gccgccttaa agctgacggg gattttcttt
    37621 ctatcatttt ggatagtgat cggtccttca taagctgcga tgtcaacgat ctcatattct
    37681 tcgttgatat tcaggtcaat agtgactaag taatccaaca attctttttc atcaagcact
    37741 cgtgcgatac gcacttttgt accgacagga taggactcca atgtttgtcg gcgttcttca
    37801 tggacaactt cctctttttt aggaatcgat cctccatgag gacaatgttc aggatgattc
    37861 aaatagtttt ctaaatgatc tgcaagatgt tccgaagtca catgttccaa tacttcagca
    37921 tcatcatgga cttcattcca gctatagtct aaatgttcaa ctaaaaaaac ttcccataag
    37981 cgatgtttgc gaatcagact gctagctttt tgcagtccgc ttgcagtcaa ttgtacacct
    38041 tgataagggg aatgttctac aagctcttct ttcacaagtt tagaaatcat ttcactgaca
    38101 gatgcagctg agacatctag actagatacg atttgtttat tgttgacttt cgttgtatct
    38161 ccacctagtt ctaaaatgat tttcaaataa tcttctcgat ttggtgtcat attcgatcat
    38221 cctttcatgg taaaaaacca tcattagtaa gtgtaacaaa aaaaacgtta attgactatt
    38281 tttctaaata aaaaacaaag gatttttaga cgtttttaca acaaaaaacg aaagatgata
    38341 gagaagactc attctttcgt ttataaagga tatatatata cattgggagg gatctatttt
    38401 tcagataaca acttatatga agtgtctgca gattcgtcat aaagagtttg ttcgggataa
    38461 gaaaagataa gataaaaacc atactgatca cgtacgacta aacgatattc atctatttct
    38521 accacagatg tgccaagaga ctgaccgttg atcaaaaacg tgtaatcaga tgtaatctgt
    38581 aattgttgtt tttgctggct atcactaaag aaccatgtac cttgatactt tgagagtcct
    38641 tctgactttt ccgcttgttt tttctttttc aagtaaataa aacttgagag aacggtaatg
    38701 agtgaaccaa gaaaatagat catttttttt atcaccaaat cactccttta tgagaaaata
    38761 atcgcataga catattcttt cttctttatt tcattgtacc gaataaaagc taaaatttct
    38821 ctaagacttt cgactggttt ttttactgtt tttctacaaa ttatgatagg atacatagta
    38881 ggaagaaagg tgacataatt tgaagtatca gcaagtggaa atagcttttt ttattgaccg
    38941 cccgaatcgg tttatagcac attgtatgaa aaaagacgga gaaataatta ttgcgcatgt
    39001 caaaaataca ggtagaggaa aagaggtcct gataccaggc gctgaagtgg ctattgtatt
    39061 tgctcctgga cctaaacgga aaaccgccta tgatctgatt gctgtaaaaa agaagggtga
    39121 ctggtttaat atcgatagtc aattgcctaa tcggctggca attgatggta ttttagacgg
    39181 cacgatcctt ttaccaggat taactggtcc atttacggta tacaagcgtg aagtaactta
    39241 tcttcactcc aaatttgata tttatttaga gacggtggtc ggacaaaaag catttgttga
    39301 agtcaaaggt atgacacttg aaaataaagc gattggtgct tttcctgatg cgcccacact
    39361 cagaggattg aaacatatag gagaattaac gtatgccgca caagatggtt atgccgctta
    39421 tgtcttgttt attgctcagt tcgaacatct tcatctggca acgatccatg aagaaatgca
    39481 accagcacta gctgatatgg ttcgacatgc gcagcaaagc ggagttcaaa tcctcgccta
    39541 taattgtcaa gtatcggcag atgaggtaac tgtcaagcag gccattccat ttgatgtaga
    39601 tatccctttt gaagatccta tacaatagaa agaaggtaaa ataatgatca acttaggtat
    39661 tatcggaaca aattggatta ctcatcaatt tgtccaagca gctttaagca caaaaaaata
    39721 tgaattgaca gcggtatatt ctagacattt ggaaaaagca caggaatttg gttctcatta
    39781 tgaaggggat atcgaattcg caacagattt gaagactttt ttcaatatta ctcatatgga
    39841 caccatttat attgcttctc cgaatagtct tcactttagt caagctaagc aagcaatttt
    39901 ggcaggaaaa aatgtgatag ttgaaaagcc agctttttcg acgccaaaag aaatggatga
    39961 aatcatccat ttggcaaatg aacacaatgt gtattttttt gaagcagcaa gaaatatcca
    40021 tgaatcaggt tttaaaaaga ttgccgatta cttaccatta aaagacgaga taatcggtgc
    40081 aaatttcagc tatatgaaat attcttcgcg ctatgaccaa gtattagatg gagaagaacc
    40141 gaatatcttc tcgactcatt tctcaggtgg tgcattgatg gatttaggtg tttaccttgt
    40201 atatgcagct gttgcttggt ttggaatgcc aaaagaagtc cattatttcc caagaaagat
    40261 ttctactggt gtagatggtc ttggatgggg gatcttacgt tatgagaatt ttgatgtatc
    40321 gatccagcca ggtaaaatcg gtgattctta tttaccgtca gagatttatt ttgactcagg
    40381 aacgctagtg atgaatggag tcaatgcaat tgaacaagca caatttcatg atcgcgcgca
    40441 taaagaaatc attgatttgg cggtaacagc tgcagaaaat ccaatggcag atgaagcaga
    40501 ggattttgcc aatgtcatgg ctcatccgaa agatccagct tggggattgt tgtatgaaga
    40561 gtggatcgag ctagctagaa atgtgaacca agtgatttat gatttaagaa aaaatggcgg
    40621 catcaaattc gatgcagata atgaggaaga tttttaatga acgaatttgt acaagtattt
    40681 cccgaaaatt ggcagaaaaa atatgaggat aaagggttta tctcaccttc tcttattcaa
    40741 cgagcagttt ttcagccact aagtaataaa caaagtatag tagcggtttc tcctacaggt
    40801 tcaggtaaaa cactggctta tctttggcct ctactactaa acgtggaacc aggtgaagca
    40861 agtgcgttgg tcatttttgc ttcttctcaa gaactagcta ttcaagtagc agatgttgca
    40921 cgtgaatggg gaaaagacaa agagttgaaa gtccaatctt tggtgggcgg agccaacgtc
    40981 aaacgtcaga ttgagggttt gaaggaaaaa ccagaaatct tgattggtac accaggaaga
    41041 atattggaat tgatgaaagc aaaaaaaata aaagcacatc aagtgaaaac aatggtattt
    41101 gatgaagcag accagctatt tgacgctggg aacagccaaa tcattgatca aattcttcat
    41161 caagctccaa cagagtatca gctagctttt ttttcagcga cagcagatcg ttcccttgaa
    41221 agcattgaaa aaatcactgg gaaaacgttt gagacaattg acgtgacagc agaggacgat
    41281 tctcgaaaag gactgagaca ttacttttta cgtgtaccga tacgaaagaa agatgactac
    41341 ttgagacgtc tcacgcatat agaagatttt catggtctga ttttctttaa tcaactgact
    41401 gaattaggcg taatggaaga aaaattgctg tatagaggtg taccggtagg aagtctggcc
    41461 tctgatcaga acaagctact ccgaaaagca gcgctagaac aattcaaaaa gaaaatggtc
    41521 agagcacttt tgaccacaga tgtggcggct agaggtttag atattacagg tcttccttat
    41581 gtagtgaatg cagaagttcc gttaagtgaa gaagcttatt tgcatcgttc gggaagaact
    41641 ggtcgaatgg gtaatgaagg aatagtaatt acactcgtac aagataataa tctaagagat
    41701 ttaaaacggc ttttacgtcc atcaaatata acacttgaag agattttttt atacggaggt
    41761 aagctgcaaa ctgagcagcc aaccaaagaa gatgcagctc ctgcagtaaa taaaaagtat
    41821 gcgaaaactt tttctggaaa ggagcagaac ccagaatata acaaagaaaa aataagaaaa
    41881 aaagttaaaa ataaatcaaa aaaagagaga aataaaggag caaggcgcaa ataagcgcct
    41941 ttttatgcta tgattatgaa aacgggaaca aaattggttg ccagaggagg ggttatgtga
    42001 aagaacgtca agaagagatc ttgcagctat tgacagaaaa ccaacaagga ttgacagcaa
    42061 gtgatgtagc tgagcgctta actatcgatc gaagcaatgc aagccgatat ttaagtgaac
    42121 tatacaaggc ccatcatatt gtaaaaaccg ctggccgacc agtcgtttac tctttgccaa
    42181 cggaaaagtc aaaatctgat gaagtgcatg tcgactcttc tacgcaagtg acttttgaaa
    42241 cactcgttgg agaaaatgat tctttaaaag tcagcataca gcaggcaaaa gctgcgattc
    42301 tttatccacc gcgggggttg catacgatta tttttggaga aactggtaca gggaagtcga
    42361 tgtttgctga atgtatgtat cattttgcaa tcgattcaga gatgctttct gcagatgcgc
    42421 catttgtttc tttcaattgt gcggattatg ctcaaaatcc gcaactgctc tttggacaca
    42481 tctttgggat caaaaaaggg gcttacacag gagcagctca agatagtccc ggtctaatag
    42541 ccaaagcaga cggcggtata ttgtttttag atgagatcca ccgtttaccg ccggaagggc
    42601 aggaaatgct gtttactttc attgataaag gtatttaccg tccgcttgga gaaagtgcac
    42661 aagtacatga agcatctgtt cagatcattg gggcgacgac tgaatcttca gaaagtttct
    42721 tatctacgtt caatcgacgg attcctatgg caatcacctt gccgaatctt gcttcacgtt
    42781 cactggatga gcgttatgaa atcatctctc tttttatcaa acaagaagcg aatcgtttga
    42841 atcaacgtat cgatatcgaa aaagaagcga ttcttgcctt catgctttat gatgcggaag
    42901 gaaatatcgg tcaagtgaaa cgagacttga aactagtctg tgcaaaatct tttttacatt
    42961 accgtactca tcatgagaaa aagctaatca ttcgaaaaga agaactttcc cttcaagtac
    43021 aaaaaggatt actgaaaatc aaggaaatac cggaacgtct cgaccgattc atggacagca
    43081 aaagtcagta tttgacattt gaaccaggat ttgctgatgt tgtctggtca caagatccag
    43141 aacgaaacat gcaggtctat aacgatattg aggaaaaagt cacttctctt tccgaaaccg
    43201 gtgtggagaa cattgatcta gagaccctca tttcaaaaga tgtggatgct tatttccaga
    43261 catatgtcaa agaattgact aaaagttcga ttcccaaaga tcttttgcca gatgaaatct
    43321 ggcggttaac caatcaattg tatgatgtgg ctgaaaaacg gttagaccgt atttataatg
    43381 aaaaagctcg ttttgctttt gctttgcatc tgcaaagtac attggatcgt gtgagagaag
    43441 gtcatatgat cgtccaccca gatttgaata acgtacggaa aaattttaag agggaattcc
    43501 aggtagcaat cgatctttca acgatcattg aagaagaaga acaagttgag attccttttg
    43561 atgaaatcgg ctttattagt atgtttcttt ctatcaatct aggagaaagt gagcctgtta
    43621 aagaaaacaa agtagatgtt ctcatattga tgcacggcag acagactgct tcaagcatgt
    43681 tggagatggc acaagaacta ttaggaacaa aagtagggaa agcttttaat atgccactgg
    43741 ttatggaagt tcaaacaatg tatgaagaag tgttacaata tgtcaaaaac tatcaagagc
    43801 atttgacgaa tggcctactc ttgttaacag atatgggatc attaaacacc ttcagtgatt
    43861 tgatttatga agagactggc attcggacga aagctatcac gatgacaagt accatgattg
    43921 tgatagaagc gatacggatg gcaagtgttg gaagaagctt agaagatatt tatcaaaata
    43981 tccagctttc ctttgaatcg atcgtacgtg atcagttcaa agcggaaaag tctgaaactt
    44041 tacagcgtaa aaaagccgta atcgtgacat gctttactgg tgaaggagta gctgccaaat
    44101 tatatcagcg tattatgcca gtgattaatc aaaataaggt agaactgatc cagatgcagt
    44161 ttttagaacg tgaatcattc aaaaaacaca tcgatgcctt attggaagaa tatgagatca
    44221 aagcaattgc gggaacagtt gatgttgatt atcaaaatat cccatttttt tcagcctatg
    44281 acgtttttga tgatgaaaaa ctaaatattt taaagcgtat agctgatgat gagatcccga
    44341 cagctaaaat tgtcgattca cttgctggat cattaagcga tgtcttttct attgatgaac
    44401 tgatccgttc attgcaaaag acagttcagc agatccagac cgatttgtcg attatcgtgg
    44461 aacctggagt agatactgga atcgttatcc atttagcctt catgttagac ggattgatca
    44521 aacaagaaag ccatcggcaa tttgagaaac tgtctatttt ctcaaaaatt tatcgtttgg
    44581 aaatggatat cgttcgggca aacttgatgg cactagaacg aaaatatcag gtaaaaattc
    44641 cagaagatga tatcgcattt cttacacaaa tgtttcttga aaataaaata aaaaaatgaa
    44701 cacactaatc atactgttta cacactaaac atctgtgtgc gcactataaa aaagagctaa
    44761 aaagcatgaa aacgctattt cagctctttt ttttattggc atgcttcttg cgttatataa
    44821 aagtgtaaaa aaataaaagg agtgttaaaa atggcaaaaa aaacaatcat gttagtttgt
    44881 tcagcaggga tgagtacgag tttattggtc acaaaaatgc aaaaagcagc agaagaaaaa
    44941 gggatggaag ccgatatttt tgcggtctct gcctcagatg cagataataa tttagaatct
    45001 aagaatgtcg atgtattatt attaggtcct caagtacgtt tcatgaaagc tcaatttgaa
    45061 caaaaacttg cgcctaaagg tattccatta gatgtcatca atatgcaaga ctatggcatg
    45121 atgaacggag aaaaagttct tgcacaagca gaaaatctaa tgaaataaat caagtatgca
    45181 gttgggggta taacaacgtg gaagatcaac aaaatctaga agccatcatg gggttaatca
    45241 tgtttggcgg taatgcaaag agcgatgcaa tggaagcgat tgcggctgct aaaatcggtg
    45301 attttgagtt agcggatagg aaaatcgctg atgcagaaga atcacttata caagcgcatc
    45361 atgcacaaac agacatgttg acacaagaag cacaaggaaa tcatatgcaa gtgacattat
    45421 tgactgttca cagtcaagat cacttaatga catctattgc gtttactgat ttagcaaaag
    45481 aaatcatcga actttatcgt cgcgtataca gcgaataaat acaacttctt ttttgaacgg
    45541 aagaagaaaa atttttgaac ggatttttta ttttgttata aggagaactc tctttatata
    45601 aaaaacttat aggaggaagt aaaaaatgaa cggactatca agttggatgg agaaatacat
    45661 cctgccggtt gccgcaaaaa tcggagcgca aaaacattta gttgctttgc gtgatgcttt
    45721 tatcggcaca ctgcccgcaa caatggcggg atcagtcgct gtaatgatca atgcaattat
    45781 tcgtgattta ccgcaacagt ttaacagtac ctatgctgac agtttagcag cagacaaagg
    45841 aatctttgcc gtattgaatg tcatcatcgg aatcaatggt tttgtctgga acggtacact
    45901 agcaatcgcc ggattgatct ttgccttttc ttgggggtat aatcttgccc gagcttatgg
    45961 agtaaatgat ctagctgggg ggatcgttgg tttagctacc ttgattcaag gtatttcctt
    46021 tggtccagca ttatcagcag cattagatgt gaatgttcct tcaaatgtcg cagatgcaat
    46081 caatagttct gagattggtg caacgattgc agatggcaca ctgtcagttg gattatgggg
    46141 ttggctaaaa ctagatcatt tgaatggaaa cgcttatttt acagttatga tcatgggagc
    46201 tatttcagtc attatttttt gtaaattgat gcttgccaac attacgatca aaatgcctga
    46261 ttcagtacca ccagcagtat ctaaagcatt tgcagccatt ttaccagcga cgatcgcttt
    46321 atacgtcatt gctattatca attatatcgt aggtcgtgta gctgatgggc aactgatcat
    46381 tgatttggtg caaaaatata ttgcagaacc attcctagga ttatctcaag ggttgggagc
    46441 agtattgatc gtcacagtat ttgttcaagt attctggttc tttggtattc atggaccaaa
    46501 tgtattggca ccagtgctag agggaatctg gggacaagca caactaatca atattgatat
    46561 tttccaaaaa ggttataacg gaaaaactgg tacaccagcc gtgttagatg caatcgaaga
    46621 tggaaaagct tatatgtggg tccgcggatc gttcgatgct tttgcatggt ttggcggatc
    46681 aggcggaacg atcgtcttac tgatagctat cttgattttc tctaaacgtg cagattatct
    46741 aacagtagct aaattatcca ttgggcctgg tatcttcaat atcaacgaac cagtgatgtt
    46801 tggtctgcca atcgtattga atgcaatcat gtttatccca ttcttgattg ctccagtagt
    46861 agcaacagca attggatgga cagctactta tcttggccta gtcgctccgg tatcgcagca
    46921 agtcacatgg gtagtaccgc cgttcttatt gtcattctta gcaacaggag cggattggcg
    46981 ggcaccgatc gtaacattag tatgtatggt cgtgactttc ttgatttggg caccatttgt
    47041 catctctgca aacaaaatgg accctgccgc aggcgaacaa caataagatg aacgtgagga
    47101 gcgggacgct ttaagtttcg ttcctcataa tgaattttcg aggaggaact gaaatgatta
    47161 caattgccag tgcaatgcaa caagatgaaa tcaaagaatt gttagaagga tacaatgaag
    47221 aaggaatgac ttttacattt aaagaaaaac aaggaatcaa gctgttcttt gaaacaagtg
    47281 cagaagatcc ggaagttgcg gctaaaaaag caaaggaact aatcaaagca cagccttggg
    47341 gaacagtcct ttattttcaa tcaacagctg tttagaaaat ggtttaaaaa gataggatgg
    47401 ttgagatgat aaaaaacggt ttatttttat gtagtattgg ttctttgatc gtgttgtata
    47461 gtttgaatcc acattcgcaa aggtatgatt tgtatagtat ggtcacaggt ctttttctaa
    47521 caggattagg aatctatttc ttcatgaagg ggaaaaaaag agaagaagca aaggagaaaa
    47581 ataactaatg gaaatcacga aaaaattaaa tattccagca tcgttttttt acgataaagt
    47641 tatggactct gtccttttcg atgtacgtaa agcgaccggc aaatcagtaa ctagaaaaca
    47701 gctgaaaaat ttggagtacg tcaaacaatt ctcgcaaaat aaccgggctc gcataaaaat
    47761 cgaagaagtc attgaaaacc agtcttataa gtttcgtaca tcgacaacga agaatgaatt
    47821 cgtcgttcat tatcaaatca aaccattgga cgattcgacc tgtgaggttc attacacaga
    47881 aaaaatggag tcttatggca tgcttcaaaa gttgaatgat atgcttttag gaacaatctt
    47941 gatgtatttt aaacggagac gtttcaaaaa aatgctagat atgatcgaag caacgtatta
    48001 aatcaatgaa acatggtact atttagttat tgaaaaatga agtaagctag aaggaatttt
    48061 tcataggaag atagatcttt cctgcgaatc ctcactagct tttttgtagg aaggggcagc
    48121 aaaatgacaa acaaagtaag agagctaggt gtatcttcga ttgaggaaat gtttgattta
    48181 gccacttatg catttaatgg ggcagataca cctgaaaggc gagaacgttt tcgtacactt
    48241 gcggagaatt cttggaatta cggatttttt gatgaggagg atcggctaac aagtcaagtc
    48301 atggcgaccc cttttccagt gaatttttac ggacaagagt acctaatggc cggcattggc
    48361 tatgttgctt cttatccaga agcaagggga caaggcggga tcaatcagat catggaaaag
    48421 atccttgaag attgccgtga tcgtaaagta agcttatctt atctggcgcc attctcctat
    48481 cccttctatc ggcgctatgg ctatgaacaa tcatttgata aaatcagtta tcaactaaac
    48541 agtcgtgatg tgccgtttat caaaaaaaaa tctggaaaaa taaagcgtat ggattttgaa
    48601 gatgcaaaag ctgcaatcag aagaatttat gaacagatgc cggaaaacca acgagctggt
    48661 ctgaagcgcg aagactggtg gtatacgtat aaattcaagc aaaaaggcaa ttaccagttt
    48721 gctttgcatt ttgatgataa tgaggaagca acgggatata tcgtttatca gcttgcaacg
    48781 tcttccttta ttattgcgga gtggggattt ttggatcatg atgctttttg cagccttgtt
    48841 cgcttcgtaa gttctcacaa tggtgctttt gaaacatttg aatattcatg cggctacggc
    48901 ggtaatgatc aaaattatct attagaaaat ccatttgctt ctgtaatgat cacaccttat
    48961 atgatggcga ggatcgtaga tatttctttg tttttggaaa attatccatt taaaaagaga
    49021 caagctgcat ttgccctaca aatagaagaa gatcattatg cgaaatggaa tgaaggaatc
    49081 gtggaagtaa caatcgaaaa tggaaaaaat catctctcaa aagtagaaca aacaaaactc
    49141 ccaatcgttc gaggaagtat ccaacatatc acgcagctat tattagggta tcgtaaagtg
    49201 gaagagttgg tattccaaga aagaatacag gtgacaaaag aatatgaaga aacgttaagc
    49261 tcaatcttgc cacagcaaca gccaattctt aatgactatt tttgataaaa aacgggtgat
    49321 gacaatattt ttgtcaccac ccttttgttt ttcatttggc atttgaagga aaatgttaaa
    49381 gtaaatcctc ttttttcgta aatttgaatt ttttgttttc ttttgtttta gggttttctt
    49441 ttttgtggtc ttggattgct tctttcgtaa tcttttcccg atttttttta gatgaatcta
    49501 agcgttctgc gtatactttt tcttttggct tatcattcat tggaaaggac ctccctacat
    49561 tatttcattc tacctctagt ttatcatctg ttaaggacta gtggaaacgt tttgcctaac
    49621 ttataagctg atttttttag cagataagct tgcaagtaaa aaacaaacaa gctacaattg
    49681 atttattgaa attgttcctg tagctcagta ggatagagca atcgcctcct aagcgatagg
    49741 tcgctggttc aattccagtc aggaacgtat aaataacccc tgtatccttt gcggtacagg
    49801 ggtttgtttt gtatttgtgc caaaaatgtg ccaaactagt tatcattttt atataaacta
    49861 tcgaacacag tattgatttt ttctttatcc tgatcttcaa gctctcgaac aatatgaaga
    49921 taagtagaca ttgtagtttc taaactgcta tgtcctaaac gtttcgatac gctaagtata
    49981 tttactcctt gataaagtaa aatagatgca tgtgtatgcc ttagtccatg caaagtaaat
    50041 tgcttttcaa tccctaattc ttttaatttt ttgcgcaaat atttgctgac ggcgtttgat
    50101 gaaactaatc cattttttaa gttgaaaaaa acaaaattat gagggttttt gatttcaaaa
    50161 ttctcgaata attctttttg attgatttta aactttttta agagatcaat cgtatgttta
    50221 tcaatggaaa ttttcctatt tgaagtttcg ttttttgttt ttccccattc atttaattta
    50281 taattccatg ttttattaat agtgatgatt tgatcttcga aatcgatatc ttcccatgtt
    50341 aatcctagaa gttcagcaaa tcgcattcca gtggcaccag caactaacac gagcatagga
    50401 gaagagtact gcgcagaaag gttttcttct acgagtttca ttaaagattt aaattgatca
    50461 taatctaaat atttatcttc ttttggcttc aatgaatctt ttcctttgat tactgctttt
    50521 ctagttggat caaacggaat taatccttct tctacggcat ctttcaacga cgctctaata
    50581 tggttattaa acttaataac ggtagatttt acatggtctt tagcatactt atttaaaaag
    50641 cgttgatatc ccactctatc taaatcagaa atcaataccg ctggcatata tttctttatg
    50701 ttcattaacg tatcttcgta ttttctataa gtgataggag atactgttcc ttctttataa
    50761 agttgcatcc aatcctcaaa gtaatctgat aataatagat ttttgcgatc ccctttaagg
    50821 cctttgttca agttatattc taattcgttg gcggcatctt ttgcttctcc ttttgtctta
    50881 aatcctgatt ttcttatctt cgtgtatttt ccgtcatctt ttttgtatga aatttcgtat
    50941 tgccaactat ttccacgttt gactaatctt gccataattg aatcagctct ctttctctga
    51001 tacaatagga actataaaga agcccattgt ttgggtttta ttattttttg cacatccttt
    51061 ttgcttgccg gcgcaaggat gtgctatttg tcgttttaat ggtataattg gatgtgtatt
    51121 agatcatcca atcagaggtg tttattatga ttaaagtgat ttttaaaaga ataactgaac
    51181 ttccagagtt tagattattt gttgattatt ggtatgtctg gttttttatt gttgcattgc
    51241 tgataggtct agatgtcata ttagaaagaa aacttaagta agagttgagt taataaattg
    51301 aattcctaaa tgagatattt tgtagcccat attttgtttg ccgacatcac ctaatactat
    51361 ggagctaatc gttttttcta acagaccaaa atcagataat ctatttaagc taccatctat
    51421 tagctcttgt gaatagccag acatgctgat atttccagtg aaattttcgt ttccacgact
    51481 cattaagcat gctaacaatt gaatctcaat cgatgttaat tgattcagta caagaagata
    51541 atactcctcg ttatcccatg aagcgttgtt aattcgaagt aaacaattct tataaagatt
    51601 tttatacata tctattttat tttgtgcctt tgatttttct acctcgtcat ttatcccttc
    51661 gaatattcca atgagttggt ccttgttctc taagttgaaa acatcttctg gaatttgatc
    51721 gcgaatgttt tctaatgatt cgtttaaatt tttgtagaat ttctcgatgc gtttgaaacg
    51781 cttctcgttt ttagacccaa aataaagtga ctgcacaacg ccaccaatat aaggaattgc
    51841 tccaataccg ccttcaacta ttaattcaag atattctttt ttggttagtt tttcattgct
    51901 attatccata gttacctcct attttgtcat ttcctttaca agcattgtga ctcctgatgg
    51961 cactcttttg tataggtttt attattgtgc acaatctaac tcttagtggg gcgtggttca
    52021 tttatttttt acttacttta ctattaatca tatcccattg agcataggat ttttctatgt
    52081 tcgatcctct tgaactaatt tctcctgtaa aagttacttt atcgcccttt gtaaaggtat
    52141 cagcattgtt taagtccttc gcaaaaatta catatgaaac tttaggtgtt gcagaaatat
    52201 cattccaact tttatcatcg tactctttgt ctgctataat ggctattcta gtagtcattg
    52261 attcgataac agttccttca aaagtatatg ttttaccact aatatattta gtaaaagctg
    52321 tattttgctc agtagctgga gtaatagaat aataactatc taagaaagca ccccagtcat
    52381 gattatttgt ctctaaaaat tcttcaaatt gagttaggct tttattgctg ctgttagatg
    52441 tagtagtttg agtatcagat gatgagcttt cctgtttgtt attgtcattt gcctcgtttg
    52501 atgaggaaag cgagaaacct acggtagtaa aaataatccc gataataagg agaatcatag
    52561 gtgttttctt atttgttttt ttgataaatc ttacaattaa catgattaaa cctataataa
    52621 ataaaacaat accagtaaaa ccaaatatac cgtccattaa tagatcctct ttctcttttg
    52681 atattgaata ttataaacca agcaattctt ttttcttaaa atcgaattct tcttgattaa
    52741 taatcccttc atctaacaac tctttatact ttttaatttc atctgcactt gaaatttgtg
    52801 tttctttgga attattatct aatatagatt gttgcttctc tatatatttt tttaaatttt
    52861 ccatttcatt ccaatatttt tttgtaaaca taacggtgtt ttcatctttc gtggccgcta
    52921 atatacctcc gcgactttcg tttccgccta gcaaggtaaa ttgtatataa ccattactca
    52981 tacctggttt ttttaattgt acggctgata tatttttaaa ggggatagtt ttttctcctt
    53041 taataccttg attaactaaa tttataaatc cttttctaat aattgaaata gaatcatcag
    53101 ttactttaat caaggttttt cctggagatt ttattaaaat ctctttttcc attttttctt
    53161 cctcatttct gtgatatgat tttttttgta gaagctcata tatgaggaaa gagcccgtgt
    53221 tgcagcacgg gcttttttaa tgttttgctg aaatcggttt ttttaataat tcttgatgta
    53281 ttcatacata ttgccttgcg taagaatatt ttttcttaaa atagcattgg cagacagcat
    53341 aacaagtgta tctgagctta ttctgaatag aataatactc cataaatttt tcgagattaa
    53401 actgagattc atcagtcagt tcgttctcaa tatagatatt taataaaatt agaatagcta
    53461 ttttatctgc ttctgtttca aattttgagt gaaaagttgt agaagtatcg tacaaaactg
    53521 aaaattcaaa atgagaggcg ctgaaatgtg caagttcatg agataaatga aaggcttctg
    53581 cagtttcacc gtatagattt tcattcaaaa aaatgattct gggttttgga tagtagaaac
    53641 caggctcttt catctccata taaactactt tcaaattgta ttcgctcaac atttctttca
    53701 atttcaaata catacaaacc atcactccaa ctattcattt tcctctaaag ctttagcaat
    53761 tgcaatcgct ttacgcattg tctccttaga tatttctttt ccatcaaaag aaaaaacagt
    53821 atcgtcttct gataaattca catgtttagg ggtttctctt tcttctctac ctagaaggta
    53881 gtctacagag acatcgaaat agtcagcaat ccgacttaat tcatctgaat taggagtatt
    53941 atttttccac tttgctagat aaccgttaga atatccaaaa ttaatttcta actgccgtat
    54001 agatatcttt ttcctcttcg ccaagtcttt tattatttca taggtattca tcgataaaac
    54061 aacctttcta aatgtttaca aaaaaagttt agaaaaatac gcagaaataa tttgactaat
    54121 tctgagtaat gagctatact atatcttgta aacagattta acaactaaaa agacaacaaa
    54181 aaacactatt gatatgtaaa tgcagaccgc caagaaagct ttaaaatcaa tgtttttatg
    54241 tcttatttaa ttatgctctt agtatagagt attactcaga tactgtcaat tgaatttaga
    54301 aaaaagttgt taaatttgtt tacgaatata aaagaaagga atgagaaata tgaacacacc
    54361 gcaaattttt aatttcgaac aaaacgaagt tcggacaatt ttagtaaatg atgaaccata
    54421 ttttgtaggc aaagacgttg cgagtgtttt gggttattca aacactaaag atgctttgtc
    54481 gcggcatgta gatttggaag ataagatggg gtcgcgaatc acgacctcag gtcaatcaag
    54541 agagatgaca atcatcaacg aatctggttt gtacagttta atcttaaaat caaaacttcc
    54601 ctctgccaaa aaatttaaac gttgggtaac aagtgaagtg ttgccagcaa ttagaaaaca
    54661 tggaggttat ctaactccag aaaaagtaga agaagctttg cttaatccag atacaatcat
    54721 tcaattagca actcaactaa aagaagaaag aactggaaga ttaatcgcag aacaaaagat
    54781 tgcagagtac gaacctaaaa tctcctattt agatagcata ttatcttcta cagattcagt
    54841 aacaattagt cagattgcag cagattatgg gatgtctcca caacagatga ataaattact
    54901 tcataaacta ggtattcaga aaaaagtcgg taaccaatgg ttattgtgca aaaaacacat
    54961 gaaccaagga tacacaaaat ctcatacaac tgagatcccg aaagccgatg gtggcactaa
    55021 aattgtaatg aataccaaat ggacacagaa agggcgtcta tttatctacg aattactaaa
    55081 aaaagaagga tattaccctc aaatggattt agaggaaatt ggttagaaag gagttttagt
    55141 atgactgaca ttgcagaaat cactcaacga gatagagaaa aaatcaaaga atatgtcgaa
    55201 agttcaaagt tcttaactta caccatgctt gctgaaagat ttggaattag caaaagctac
    55261 ttatctttaa ttttaaacgg taaaaagact tctgcagaag caaacagaat tatagattcg
    55321 attatcacta tgtacgaatt gtaaagggag gaacagctaa tgcaatatct agaagcaaaa
    55381 atcccaattc cagaaggcta tgtaattatc tcccaagtgg attatgagga gttaaaaaaa
    55441 gctgatgata ctggtagatg gatgacgttg ccagaagtgc tagaacggat taacagaaaa
    55501 tatgattggt ttacttctag agttttaaag aacccaagat atagaaatat tatcgatatc
    55561 gagaaaaaca aaaatggatt tgtctattat ccagttgaag gaagagacac atatctattt
    55621 ttaagaagta aaacacttga atttttagaa acaaattttt cggaaatctt aaggaggcaa
    55681 gcggatggga aaatttaata gagcgctagt gttcagcgca ccgctaatca tctacgcttt
    55741 aggactttgg ggaagcaggc aagcgttgat aggaacgatt gtttacatgg tttggatttt
    55801 tatggggctt gatgaagctg aagctgagta cagagcgaaa aagccaaccg agggggctga
    55861 ctaaaaatga atagaaaaga gaaactagaa tgtatattac tattactcag tttaattctt
    55921 tcactaattt ctctattggg aagtttgtat ttttgatatc aacaattttg tggatttggg
    55981 ttctatttag agtaaatatc atttttgttt ggtgattaag gagtttatta aaattaattt
    56041 tccctacttc aaatgcaagg taacaagata tagcactttt aggaggaata tttagcggta
    56101 cagcttcaga gtatattcta ttatcatcga ctgatcctcc tttagcaatt aatttcttaa
    56161 atggagtagc tgaccatctg cggtttaagt tagcggatga aagttctaaa ttaactaaca
    56221 tagctggttc tgttgaatag ttagaaatga ttacttttgt ataaatcata tctgcagcga
    56281 agtaagaagc attaagttca acttctagtt gagggcgcat tccttttatt tttatccctg
    56341 ttaaaaacgt actcaatata aaacttatta ttgacatcca ttgaaaaatt gttaagtgaa
    56401 aaaaattcaa aataaacacc accagttttt aactaaatta taccaaaaag gagagaagaa
    56461 ataatgcaag aattagtaat tttgaaaaat aaagaagctg tgactacgag cttacaagtg
    56521 gcagaaagct tcgagaaaaa acatcaacat gttttaagag atattgatgc actaaaaaaa
    56581 gatgtgtcca attttggaca gatgtttgta gaaggtaatg aaccagattc atatggcaga
    56641 aatcgacgag ttttcttcat tagtagagat ggatttttct tgctggctat ggggtttaca
    56701 ggaaagaaag ctatctactt caaacaaaaa tacattgaag cattcaacga aatggaagat
    56761 gttattcgca agaatactgt tcctcaaaca attgaagata tgatgatcta tcaattagaa
    56821 gaaatgaaag atgttaaaaa agatgtttcc atgcttaaag atactatgcg aattagcgga
    56881 caacaagagt ttgaaattaa gcaaaaagga aatatgaaag ttatgggagt tctaggggga
    56941 aaagaaagcc gagcttatga agaaatcagc aaaaaagtat tctcaaaatt ttggtctgaa
    57001 tttaaacgta ccttttcaat cccaagatat ggcgagttac ctcgtaagag attcgatgat
    57061 gctgtttcat ttattgaaat gtggttgcca gaaactgcga tccgtatgga aattgatcaa
    57121 ctgaacagac aacagagact ttttggtgat gacaatgaat agagctgaag cgctaagaat
    57181 agggacggca attgctaatt gctggtggaa atactataaa ccaatcatcc taagccaaca
    57241 acatattgac aagcaaaaag catggcaaca aataaaaaag tgactccgcc ggcaagcaaa
    57301 gagtcacaaa gaaaacacat cataaggaga ttttagcata tggaaaaaga actttccact
    57361 ctagatcaat atttgattga tcctgattgg ggcaagccga aaattgagga aacaagtggt
    57421 cgaaaaatca gacgaaatct tttgacgaat gaagaactag cttgggatca agatgattta
    57481 ggcaaccatg taactatttg ggatcatgtt tatcttatcc atctatcgaa gcattcgaat
    57541 aaacctgaat atatttacgt catcgaagat ggcttgattg atgcgctaga agagtacgac
    57601 agagataact tgattgatat ctcttattac ggaccaggta agaaatacat tgctgaaatg
    57661 gaggcagaat ttgatgagtg aaatcaaagg gacaacgaac tttgaaaaac tttttagtcg
    57721 taagttaaat aaaattctca agaaaaaagg aaattttgat tatttatctt gggctcacgc
    57781 gtgggagatt atgaaaaaga atgatccaca ggcaacggta actattaatg agtacaaaca
    57841 ctacagggtt gtttctggaa ctcatcaaga ctttcttgtt gaggaatata aaccttttct
    57901 tatggatgaa actgggactt atgtatctgt ctcagtaacg gttaaaggac acacggaaac
    57961 cgaattattt cctgttttag attatcgaaa ccaaccagtt gttaaaccaa atgcaatgca
    58021 aatcaataac tcattgaagc gatgctttgt gaaagcattg gctctacacg gactgggatt
    58081 atatgtattt caaggggaag atattccaac accacctaga atcgatacaa agaaattaaa
    58141 catgctagag acgattctag aagctttcaa tgagcagatg ggtaaagata tgaccaaaac
    58201 cttaatcgaa tatgttaatg agcagacaga taaattaggg ctcttagctg ataacgttga
    58261 aactattgaa cagttaagct atgagcaatg tgccttgatg gagcgagcaa tagcagctaa
    58321 gagaaaagaa ttagataaga agtgatatga gtgtttaaac cattaatcga ttcatattca
    58381 gcggttctga aaaagttcaa aggaaaagac ataagcgcaa ccatcaatga ggaagtgaac
    58441 attgatcgac taaagacgat gtatgacggc tacgatggtg atcgagtcat tgaaattcgt
    58501 tttattgatc ctagacgttt caccgtacag caacgaaact tcatctatgc gctgataggc
    58561 gatattttta tcgatacagg catgccaacg gacttctgga aggaattctt ctactttcgt
    58621 tttgaaggtg tcacagggcg caaaataagc ctgaaagacg aatcgaatac aactgtgagt
    58681 gatgccaatg tcttagcaaa tatcatctta gatttcatct ttgaacatca tattcctttc
    58741 aaagaaggct atgagatttt acctgcgaat caagaatatt acttctacaa atgcatcaca
    58801 aaaagagtct gctgcatctg tggcaaaaca ggagctgaca tcgatcactt tgacaaagcg
    58861 ctaggaagac gaaagcgcaa agaagttgat catgcagagt acacatttgc agcactctgc
    58921 agaatccatc acacagagaa gcacaaaata ggtgtgatta atttcaaaaa taaatatcaa
    58981 atcaaaggga tcaaattaaa ccagaaaaca atcaaaaagt taaatattgg agggtaaaaa
    59041 tgacagaaca tcgaagttat tatgcgatta taccagccaa cgtaaggtac gacaaaagac
    59101 ttaaaccaaa tactaagttg ttatacggag agataacggc cttgtgtaat gaaagaggct
    59161 tttgttgggc aggcaatgag tactttgcag atttatatgg tgtgaataaa gagaccatat
    59221 cgcgatgggt aagtgatttg attaagtttg gatacttgaa tcgggaaatc atttacaaag
    59281 agggtaccaa tcaaataatc aataggtacc tacgaattaa tcaataccct attgacgaaa
    59341 aacgcaatac ccctattgac gaaaaagtca aagataataa tacatctatt aataatacat
    59401 ttaataatac aaaagaatat ataagagagt taccgccttc gaaaaaatcg aaggctaagc
    59461 ccgtccgtca taaatacgga gagtataaaa atgttctttt gtcagatgag cagatggaga
    59521 aactcaaaac agaattccct aatgattacc aagagcgaat cgaacgactg tcagagtatt
    59581 gtgaatcatc tggtaagact tataaaaact atttggcaac tattcgaagt tgggcaagga
    59641 aagaaaaaag tgaacctaag aacgcaagca gtggatacaa gcgcacagga agacgagaga
    59701 agcttcctga atgggcaatc gaccaagaag cctatctcaa gaaaaaagcg ctagaacgag
    59761 ctaatagaca atcaaaagca ccattctaag aggtggaaaa atgaagatcg attatctaga
    59821 actaattaat gaaatagcga attataaaaa gggcgaggaa ttagacgtcc tgagagacgt
    59881 atatgatcaa ctcgaagaag ctggaattga aggaattaag aatgatcgtt cgagttggag
    59941 taagctcaga tactatttcg cactttatat cgatacaaca caattaagaa atttagcata
    60001 taccaaatta ctatttgttg attgtgttaa aggcttgcaa aaacatctta atgaacttga
    60061 gcaggtgtaa tcagatggac ctaaagacat ttacagcaca gattgaacta atgcatcaag
    60121 aagctttaag acaaagtgta tcgtacgaag acaagtggct caacacgttc catggcggac
    60181 gtgagagcgc acttgatcaa gtactcaaat tactgaaagg agaatgtcgg gatggataag
    60241 aaagcggcaa tgcagcgaat tatcgaattg acttattcag aagattggca aaatgacaaa
    60301 gaagctgctt cagaagtgat gaggcttgga agagcgatgt gggcagacaa gagcaacaag
    60361 ccaagaccac gaaaaatcgc aatttggcac ggtgacaaac ttctagtgat agggacagct
    60421 gaacagttag caagtctcac aggcttgcac gagaaaatcg tgaggaaaag agcaaggtgt
    60481 ggctacacag acgctaagaa gagaacgttt agatacgtgg aggaatcatc atgacaacag
    60541 aagaagtgat tcaaatgcgt attcgaagcc ttcagcgtga gattgacgat ctggaacgaa
    60601 caaaggcagt gatggtcaat gaaacggcga gaaaggcaat cgatttgcac atagagaatt
    60661 taagaaggga aatccatcga ttggaggaat gagcgtggat aagaaagcag caatgaaaag
    60721 aattgctgaa ttaaccaagt cagaatcttg gcaagaagac aaagaaatag ttgcagaagt
    60781 ccaaaagctc ggtaaatcaa tgtggactga aaagcccaaa cggagaacgc cgagaaaaat
    60841 tgcaatctgg catgatgacc gaattttagt aacaggtact gctgaacagt tatctgaaat
    60901 tactggatta agcaaaaaca ttatctggga tagagctagg agcttatgga ttgattcaaa
    60961 aggacgacag tttaggtatg tggaggagag ataatggatc tcattacaca atacagtgac
    61021 atcatcctca agaaaatcat gatgaagatt cagaaagata aaaaatcaaa agaacgagct
    61081 gaattagtta agttagaaat ggctgaaaca ggagcaggag tgcgaagtag caggcattgg
    61141 aaagcagcag caaacattga attttattac aacgaaattc aaaaagggtt cgatcagatg
    61201 cgtgagctgg atcggcaaac aaattggagc aagaaacttc atcaagatcg tttcaaattt
    61261 gtagagaagt ataaagaaat attagaagag tatttgagga ggacagcaaa tgataaaaaa
    61321 actcgttcaa ttcagcatgg atttatatga tatcgaatca ggagcaacac tatctgtgga
    61381 atcggaccat ctaatcataa attttggtgg aaagcgccag attattttgt gggtagttga
    61441 tgatgtactg tttccagaaa ttgttcatga tttcgaagaa tcaaaagcgg ttgagtttga
    61501 aatagtgaaa aaagtaatgg aattgattga aaaatacgag gaggacagcg aatgataccg
    61561 aagtttagag cgtgggataa acgaaagaac gtaatgagag atgtagccgt cttgcatttt
    61621 actaaaaacg gcaaaacaaa ctttattgaa tattggataa atcctaccga attgaaatca
    61681 tatcacgtgc gaaacatcga cctcatgcaa tccacaggaa tgaaagataa gaatggtgtg
    61741 gatatatttg aaggggatat agtattagtc agcgtgcgaa atggcttcga ttacttagat
    61801 aataaagtct gtattgtcaa aaattcaata gattattccg gattagtttg tgccactgtt
    61861 gatgaagact tagagtatca aatttttaac acagagctgt ttgaagaata cacgtacgaa
    61921 gtcaccggaa atatatacga gaatagcgag ttattggagg aacagcgatg aataaacagg
    61981 aagcaatcaa aaagttagaa agtattaaag cgataggaaa tgatgcaata gctgcttgct
    62041 ataacgagag tataaattca ggtattacgt taatgaaaaa aatagacgaa ccgcagaaac
    62101 cagttattcc acaactcgtg gccggttggc ttgagaaatc tacggaccct tttacaaaag
    62161 ctgaaaaaat agcgtattta atcaaatcta aagatggtga ttcatattat ttctgtgatt
    62221 ggtttgtacg agatggcata gtgacgcaag agcaaggaga agaattactc gcttgggcaa
    62281 caagacaatc atatgaaaca ctattgagcc tatacaacgg ctacgaggtt gagaaagagc
    62341 cgttatgggc aataaagaat gccgatggaa actatcttac taaatgtgct ttatggggaa
    62401 aagatggagt aaattatagt tttgaatgca atccatctca tcgattgctt ttcactgata
    62461 aagcaacagc ggatgctgca gcattgttgg tgaatggaac agtggaagag gtggtagaaa
    62521 gatgaaacta aaagacggat tttacgctag cagtcacggt atcggcggtt taatgctaga
    62581 tatgccgaca aagaacccta aaacacgcaa gaaatcaaaa ttcaaagtcg gtgacatggt
    62641 ccgctgtgaa gcagaagagt tcgtttatcc gttcagagga tacgttaaaa agatactgtc
    62701 aaactcagca atcattcgta ttgaaaatac aatgaagtgt gatcgttcga ctgcaaaaca
    62761 caagcattat ttagctgtag cgagattagt tgacatggaa gtaatcaaga gcaaataaaa
    62821 aaagccggat cgctccgact gattcaataa atccaacaca tttattatat cacataaagg
    62881 agcggtttga cttgatgcaa ttgttacgag aggtagattt caaacagaca agatgtaatg
    62941 cgagagatgt gctgaagaac tttcggcgtt tggagcggat ggcaggtcgc tctttgatag
    63001 atattaagtc gccgattata acggatatgc cgaaggcacc gaagcatggc aataaggcag
    63061 aagacgcgat tattcagatg atggatatag aaacggagag agatgcgatt ctagcggctt
    63121 tgatggctct tagtctgatt agtcgtcaga tactctacta cagcttctgt gtgccagata
    63181 gcttctcaaa ctacagaatt agccgtgaag tgggttattc agaaagaagt atacaacgga
    63241 tgaagtcgga agctctaata gagtttgcag aagcatataa acacggaaaa ataattgctt
    63301 ataaataatt tggcggtttt ttggcggaat gatggcggtt tttagacatt taccagtgtt
    63361 attatggtag tgtcgaaaga ttaggaaaca ggatcgacaa aataaacgaa gggaggcaat
    63421 ctccctcatc gttttaaact tcttcttaga tagacagcaa ctaattatga agaaaaggat
    63481 gtgaattcaa ctcctcctaa attcttcatc ggttgctgtt tattttgatt agacagcagc
    63541 actcacaaaa ctaaacatgt tcttgtcgtc tttcaactgc tgctgtctat taatttatgt
    63601 attggaggga agaaaaatga aattatcagt agaaggcact ccagaagaaa tacaagaatt
    63661 gctccaagct attggtagta gcaaggagca aaaggtaagt gtttctaatt cagatattga
    63721 cggtatttgc aattgtttaa acagagaaaa aatatctaat ccttactaag agaaaatatg
    63781 ttcgttaatg aatttccaat tttcctctgt agctctacct tgataatttt tttgcagttc
    63841 acagatgaat aaggaatcgt tattatcaag tactgtattt aatttttcac acatttgatt
    63901 tggattcaaa ttagttttaa ctaaccaaaa agatttttgt agctttatat atgccccaaa
    63961 ttcttttatt atttcaaata ctttatcata cttttgccct gggttattaa gatcataact
    64021 aatcatgtag ggtttataca tgttgtcttg cacatccttg ttttatattt cagcggacca
    64081 ctcgctgata aataaaatta tacgcttagt atttattttc acaatattaa tttatcgtaa
    64141 taacttttga gtaaatagaa caaaaaacct gcactagttt ccgctagtac aggcagtgac
    64201 tatatcggtt gataatctag catatattaa aaataattgc aagaaaagaa atttatagtg
    64261 gtttatacca aattatcaaa gcaatgttat tttgttgctg tctattgttt tttaattatt
    64321 cacacgataa ctaaaggtgg gtgaagagaa atgattccat taataatttc aatttttgcg
    64381 ctctgtctta atgtctatat gattggattt aaaaatgggc aaaataaaaa atagtagcag
    64441 ccaagaataa ttttatagtg tcactgtggc ggaaagggta gacgctaagc atgtgtgcta
    64501 ggtcaatgct tcggcaacca tgcaaggttc gattccttgc cagcgacatt aaatgcctat
    64561 gacggttacg actaccgaaa aaagatcgtt aagaagctat acggtgctac gtacggcaat
    64621 gtagtaagtg tgctatctgt acaccaccaa gcttcggtca ctgtggcgga agtagaagac
    64681 gcagcggtaa atggcgagta gcctcgtgag agcctggtaa gttctcgtga gtggtgcaat
    64741 cccactccag cgactttaag caaccgaggc atcggcggtt taaaaatata ggggtgcgca
    64801 atttcgtacg cgttttgtgc atcgtgcaag ttactattac atattagatc actctttgag
    64861 tggtcttttt atttttgcac aaaggaggaa acaacaatgt atagaccaca atacttagaa
    64921 cagaagtatg aagtaatcac tgttcataaa ggtaatggcg aaaaagtata tgagtataga
    64981 agaccaataa agagcgatac atataaacga aaggaaaaca atgaagttat tccattgtat
    65041 ggcaaaagaa tagctaagca ttaaataaga ttgcgaaagg agacggaaca tgaccgagga
    65101 attctataga tggctattac agttgacaag agaagatcgt ttggttaagt tctatcagtc
    65161 tcctaaatgg cgcaggctta gagagaaagc gatgaaacga gatcactatg aatgccaaga
    65221 gtgtagaaga ctaggtaagt atcatagagt agagaacgtt catcatataa aggaagtcaa
    65281 ggatagacct gacttagctt tagatttaga taatcttatt tgtttatgtg ttgaacatca
    65341 taatgaagtt catggcagat atcttacagc attagataaa caagagaaga agatagaaag
    65401 ctttgctaac ttcgatgcaa gtgaaaggtg gtaagtgcat gatcatcaat gataatggca
    65461 gagagtatga tacagaaaag attgaagagt attcatctta tactcaggga ttaattaaac
    65521 gtttgatata cgttcgctat gtaggtatta gggatctgtt atcagataac tgttgtagta
    65581 aatacaaagt gaatcaagta agagaagcgt tgaataaaga taataacgtc gaaagaataa
    65641 aaaatgtttt tggatatagt attgaagaga ttaattatta cattgacttc gctgaagctt
    65701 tcattccgat ggtgagataa ccccccctta aaataaatcg caaatttttt gggggtgatg
    65761 aaacggaggg ggctgtcagg aaaagagatt ttttcgaact ttatcatgaa aggagggcta
    65821 aaatgtttaa aaacgaattg tctcaaaatc gctacagaga aaaattacgc cgctctttaa
    65881 taagccaatt ggaaagtcag aaaacaaata ttgagccatt cttagataat gttgatcgtt
    65941 atatcagttt atgggaaacg gcgatatcac tggaagaaga tatatccgag aacggcatta
    66001 gactggagaa tggtaaaaag aatgaatcag tagcgttgct tgtttctgtc aacaaacaaa
    66061 tgggattgat gttggataaa cttgccatta ctcctgaatt ggtaggtgaa gcaaatgaat
    66121 caattcctga gttataagca tattgaaaat tggttcaaag ctatagaaga aggcactatc
    66181 aaggtatgca aagagcaatt attgctaaaa aattatctag aagaaagagt ctttactaga
    66241 gaagatattt acttcgataa gcagatggta gaggattcaa tcaatatacc agcacaatac
    66301 tttccattcg aattaattcc gtgggaaaaa tttctacaat gttttattta tggtgttcga
    66361 tggaaaaaag ataaaacact agtgttcaat agatatcttt cattaatggg acgtggtaat
    66421 ggtaaaactg gttttgcttc ttggaacaac ttctttctac taaccgctaa acacggtatt
    66481 aaaaattatg atattgatat ctatgccaat aatgaaagcc aagcaaagac tagttttgat
    66541 gatgtattta aagtaattaa agatcatcct gatttagata aaaaagtatt taaagctacg
    66601 aaggaagtta ttcaaaatat cgctacaaac agcaaacttc gttataacac ggcaaatgct
    66661 agaacaaaag atgggaagcg accaggtgca aaccgctttg atgaaattca cgaaaatgaa
    66721 gattattcaa tgataaatgt ggctacttct ggtggtggta aaattcgaga ttatagagaa
    66781 ttttatgata caactaatgg tcatgttcgt ggtggtccgc ttgatgacat tatagaagaa
    66841 tcaaaaatga ttctttctgg agaacttgga attgacaagg atggagcaga attttctagt
    66901 ttgtttccat ttatttgtcg cttggataac gataatgaag ttgatgatcc cgacatgtgg
    66961 gaaaaagctt gtccaactat taattacaat gcagatttaa aacggaaaat gtttcaagaa
    67021 tactctcaaa tgcaacgtaa tgctggttta agacttacgt tcatgaccaa acgaatgaac
    67081 agacctatgg aagatacacg atttgctgtt gcttcatatg atgatgttct gcatacgaaa
    67141 gaaaaagaat ttcctgaaaa aatggatgaa gtgataggaa cagtcgattt tgctgataga
    67201 cgagattttg ccagcgttgg gttgctagga aaatacgata aagatgttta ttttacacaa
    67261 catactttta tccacgaatc agcccttcga ttacaaaaca tcaaacgaga ggttatagat
    67321 atttctatag atcaaggaaa atcacagatc gttcatggaa aaaatataga agctgattat
    67381 attgtaggtt ggtttcttga aatgagtaat aaatattata ttaaaaaaat cgctatggat
    67441 atgtaccgtg caaaaatatt gaagcccgct ttagaagaag caggttttac tgtggaaatt
    67501 gttcgaagcg gatctgttac acatggtatg ttaaaagatc tggttgatga cctttttatt
    67561 aatcaacgtt tattttttgg tgacgatgcg attatgcgtt ggtattgcat gaatgtatat
    67621 gaagagcata tttctaatgg aaatatacgc tatgaaaaaa tagaacctga aactagaaaa
    67681 acggatggct ttttttcatt ccttcatggt ttgaattttt tagatgatat ttatgattct
    67741 gctcctgtaa cagtcacaaa tagctcagta gaaaatacag gaactggatt tactcctcta
    67801 gtattctaac ttgaaaggag gtgagaaagt ggggattttt caaaaggcgg taggatactt
    67861 cacaaaaaaa gcaacggttc ctttagaaga atacttttgt aaattgcaag ttgattttgt
    67921 gtatcgaaaa tttgcgattg aaacttgtat tgatttgatt gcaaatgcga tgagtaaagc
    67981 ggaattcaag tcatatgaag atggaaaaaa taaaaagaat gatctttact ataggctgaa
    68041 tgtagctcct aataagaaaa ataatgcaac agaatttaga aaaaaactga tcaggagatt
    68101 aatattctac aatgaagtat tgatcgtttc tccgtctaat aattctagcg aaatatttat
    68161 tgcggatagt tgggatgtca cagaatatgc attgaaagat gatgtgtttt ctcaagtgca
    68221 aattaacaac atagtccttg atagagaatt tctagaaagt gatgttatct atataaaata
    68281 cgcagatcaa caaattaggc aactagtcga tgcgtattat caagcgtatg ggaaactcat
    68341 ttctagtgcc atgaatgttt acaagcgctc taacgctcgt agatacgtac tgaaagggaa
    68401 tttattccga ccgcaagaca atacaacaca agatcaaatc aataaaatga tgacatcaca
    68461 atttaaggct tttatggaag ctgataatgc aggtgcggta tttcaattac aaaatgagta
    68521 cacattagaa gatttcagcg gaaacttcca aagcaattca agagatataa aaaacttaat
    68581 agacgacatc tttgagatga cagcagcagc gtttcacgtt ccgaaaaacc tactaaaggg
    68641 agacatgagt gggttatcgg atcaagtgga cgctttttta atgttcgaaa tcataccgat
    68701 tgctgaactt attcaggatg cgtttaacgc tagtctctat gaagtagaag aatacttgtc
    68761 agggaatttt gtacgtgtgg atacaactat gatcaagatt actagcttca aagatttggt
    68821 tgacgctatt gatgtaggca ttagaaatgg agtatttaca atcaacgaag gaagagaacg
    68881 cgttggaaat gatcgctctg ataaggcgat ggcagatgaa atatttataa ctaaaaacaa
    68941 tcaacaagta tcgaaaggag gtgaggcgaa tgacgacaat gaaaacattt ctagcagtaa
    69001 agaatgaagg cgcagtaccg caaattttta ttcagggatt tattggttct agttggttct
    69061 ttgaagggaa tactgacaag ggaatcaaaa atattttgga tagtctaggt gatcaagaag
    69121 aaattgaagt agtaattaat tcaaacggtg gagacgtatt tcaagggatt gctattggga
    69181 acttacttaa gtcaaataaa gcaaaagtta acgttgtgat taacggctta gccgctagtg
    69241 ctgcttcaat tatcgcaatg gctggcgata ctataaaaat ctacaacaat gcacaattga
    69301 tgattcaccg cgcttccaca tacggagaag gaaatgtcga tgacttccgt acgattgctg
    69361 accaactgga atcaattgat aaatcggtaa aggcttcata taaaacacga ttcaatggca
    69421 cagatgaagc attgcaagaa cttcttgaaa aagaatcgtt tatggatgca gaaacagctt
    69481 tgagttatgg attggtcgat gaaattatcg atgcagaaaa tagctcaggt actgaagcta
    69541 aaaaagaaca aagcgttgaa gaaattttga atgacgttaa agaaaaaaga gcagaaaaaa
    69601 ttgctgcatt tacagcagca ttaaataaaa catttggaca aggagatgta aaataatgac
    69661 agttaaaaat ttaaaaggtg taacagctgc aagcgaccaa ttgatgaaag cttttaaaga
    69721 tggtaacgaa gaatctttta gcgcagctat ggtaagctta tctaaggaaa ttcaggataa
    69781 aattttagaa gaagcaacag caaaaaatca agatcaatta gtattaatga accgtggtca
    69841 gcgtgtatta actacacaag aaacaaaatt ctataacgaa gtagtgaaaa acgaaggttt
    69901 tgcaggggtc gaagaattag tgccagctac tgtatttgaa cgtgtatttg aagatttaga
    69961 acaatctcat ccactattgc aaaaaattac ttttgttaac acaactggtg taacagaatg
    70021 gattgtgtca cgtggagtca atccagcatg gtggggtaaa ctttgcgaag ctgttaaaaa
    70081 agttttagat aatggctttg atgtaattaa catgaagcag ttcaagctat caggttatat
    70141 tcctgtatgt aaggcaatgc ttgatttagg tccagtatgg ttagatcgtt atgtccgtac
    70201 tgttttagta gaatcattga gaattgcatt agaacaagca attgttgatg gtactggtaa
    70261 agatatgcca gtcggaatga tgcgtgacat gagcaaacaa actagcggag aatatgctga
    70321 aaaaaaagca gaacctatta cagctttaga tgctgtaact atgggcggtt tgatggcgcg
    70381 actatcaaaa ttcaatatcg aaggtgtgaa tgatccgatt tatcgtaatg tgaatccttc
    70441 tgatgtggtc ctaattgtga atccaactga ttactggtct aaagtattcc cagctaagac
    70501 tgtactaact gctaatggag aatacgtaca agtattgcca gtaccagttt cagatttgca
    70561 gtcaacggct gtgccagaag gaaaagcagt tattggggta gcctcagatt acttcatggg
    70621 tgtaggatct acactaaaaa ttgaagcttc agatgaatac cattttgttg aagacgaacg
    70681 catttatcta gctaaacaat atgcaaacgg tcaacctaaa cgtaacgata gtttcattgt
    70741 attagatatt agcgctttgg gaactactac tacaactaca aaaccaacaa ccacaacaac
    70801 tacaacacaa gcgtaggtga tcagaatgaa gtatattctt tgtcagccgg caatcaatcg
    70861 gtttaaatgg gagcttgaag tttgtttaac taatctgaag aaactaggaa tcaaagatat
    70921 cgtattgctt ttcagcagac acgatgatca gattcctatt ttttttgaga aggaatatgg
    70981 tgttgaagtt catgtgtacg atgatctgcg ggacgacaaa gagtatattc cttcgattaa
    71041 accatattta tggtggaaat atttagaaga agatcattcg cgtgaggacg accgatattt
    71101 ctatatcgat tcggatgtca ttttcaataa aagaattaat ttgcgcaaat tgccttctaa
    71161 agatgatgtt tggtattgta gcgactgctg tagttatcta agtcttgatt atattagaag
    71221 ctgtgaaaac ggagaaaata ttctaaaaga tatggcaaac attgtaaatg ttacagtaga
    71281 atctttggaa actataaaca ctaattcagg aggcgcacag tgggttatta accgtcctaa
    71341 agcgaattat tggaaaaagg tttatctgga ttctaatcgg ctatatcgct accttagagg
    71401 gcaaaaaaca aatatacaaa tctggacagc cgagatgtgg gcacagcttt ggaacatgat
    71461 gtatttcaat attggtccta aagttcacga ggaattagac ttttgttttg ctactgatcc
    71521 aatagaaaaa gttaaagaag taaaaatctt acacaatgct ggagtaacaa caaacgatga
    71581 agatttattt ttcaaaggga gatacgtgac ttctacgcct tttgatgaag atttatcatt
    71641 tgtaaacaag aaaaaatgct cttacgcata tgttaaagca attaaggcgg tggttagatg
    71701 acgcctgaac aagtgactga agaattgcta atagctgtga aggataatat ttacgttacc
    71761 tggaacgaag aagatgagtc aattaaaaag atgatagcta aaaatgctgt ttatcttcaa
    71821 agtaaagtga gtacaacact ttctttttct cctgaaagct tagaatacgg attgctaatc
    71881 gaaagatgta gatacgactg gaatcgtgct ttagatgagt ttgaacaaaa tttcgctagt
    71941 gagttattag gtttcattca acattatgcg ctacaagaat atattgcagg tgatgtgaat
    72001 ggcgaataat cgtagactcg aagaaacatt caacgatggt tggttaaaga ttttgacgca
    72061 aactaccaaa agaaatgaac taggaaaaaa gattggtgta gaagatacag aaatcacttc
    72121 tttaaaattt agaaatcttt ccatgagaga tagtgatata acagctatgg atgcgatggg
    72181 atcgaaatta actaagaaag taaagactcc atttcatcca atcgccaaga aatttaataa
    72241 agatcaatat tttatcgtaa tcgatagtat gcgttacaac gttatctatg ccgattacga
    72301 taatttttat atctattttt atcttgaaag tgtgggtgaa tatggtgatt gataattcta
    72361 aagaaaaaga acgtttaaat aagcaaattt ctgctatcaa aacttcctta gaagagcatt
    72421 ttggcctcaa actctttcaa gactccgtag gcgaggatga gctacctgat gattttaatt
    72481 acttcattct cgaaacagga ga
//
LOCUS       DACDUU010000006        65879 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.006, whole genome shotgun sequence.
ACCESSION   DACDUU010000006 DACDUU010000000
VERSION     DACDUU010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 65879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.006"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            27..1313
                     /locus_tag="IWL30_000407"
     CDS             27..1313
                     /locus_tag="IWL30_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9705201.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            1450..2319
                     /locus_tag="IWL30_000408"
     CDS             1450..2319
                     /locus_tag="IWL30_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9705202.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            2316..3203
                     /locus_tag="IWL30_000409"
     CDS             2316..3203
                     /locus_tag="IWL30_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9705203.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            3370..4053
                     /locus_tag="IWL30_000410"
     CDS             3370..4053
                     /locus_tag="IWL30_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP9705204.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            4050..4823
                     /locus_tag="IWL30_000411"
     CDS             4050..4823
                     /locus_tag="IWL30_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP9705205.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            4820..5218
                     /locus_tag="IWL30_000412"
     CDS             4820..5218
                     /locus_tag="IWL30_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAP9705206.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            5403..6074
                     /locus_tag="IWL30_000413"
     CDS             5403..6074
                     /locus_tag="IWL30_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705207.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMSYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            6133..6573
                     /locus_tag="IWL30_000414"
     CDS             6133..6573
                     /locus_tag="IWL30_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705208.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            6585..7253
                     /locus_tag="IWL30_000415"
     CDS             6585..7253
                     /locus_tag="IWL30_000415"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP9705209.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            7317..7940
                     /locus_tag="IWL30_000416"
     CDS             7317..7940
                     /locus_tag="IWL30_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP9705210.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            7996..8382
                     /locus_tag="IWL30_000417"
     CDS             7996..8382
                     /locus_tag="IWL30_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAP9705211.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            8595..9986
                     /locus_tag="IWL30_000418"
     CDS             8595..9986
                     /locus_tag="IWL30_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAP9705212.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            10597..10965
                     /locus_tag="IWL30_000419"
     CDS             10597..10965
                     /locus_tag="IWL30_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP9705213.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            11145..12086
                     /locus_tag="IWL30_000420"
     CDS             11145..12086
                     /locus_tag="IWL30_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAP9705214.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            12155..12886
                     /locus_tag="IWL30_000421"
     CDS             12155..12886
                     /locus_tag="IWL30_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAP9705215.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            13030..15210
                     /locus_tag="IWL30_000422"
     CDS             13030..15210
                     /locus_tag="IWL30_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAP9705216.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            15230..15712
                     /locus_tag="IWL30_000423"
     CDS             15230..15712
                     /locus_tag="IWL30_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAP9705217.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            15728..16489
                     /locus_tag="IWL30_000424"
     CDS             15728..16489
                     /locus_tag="IWL30_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP9705218.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            16620..17954
                     /locus_tag="IWL30_000425"
     CDS             16620..17954
                     /locus_tag="IWL30_000425"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAP9705219.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            18261..18746
                     /locus_tag="IWL30_000426"
     CDS             18261..18746
                     /locus_tag="IWL30_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAP9705220.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            18739..19422
                     /locus_tag="IWL30_000427"
     CDS             18739..19422
                     /locus_tag="IWL30_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAP9705221.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            19548..22193
                     /gene="polA"
                     /locus_tag="IWL30_000428"
     CDS             19548..22193
                     /gene="polA"
                     /locus_tag="IWL30_000428"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAP9705222.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            22238..23074
                     /gene="mutM"
                     /locus_tag="IWL30_000429"
     CDS             22238..23074
                     /gene="mutM"
                     /locus_tag="IWL30_000429"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAP9705223.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            23038..23667
                     /locus_tag="IWL30_000430"
     CDS             23038..23667
                     /locus_tag="IWL30_000430"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAP9705224.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            23719..24051
                     /locus_tag="IWL30_000431"
     CDS             23719..24051
                     /locus_tag="IWL30_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP9705225.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            24363..24854
                     /gene="nrdR"
                     /locus_tag="IWL30_000432"
     CDS             24363..24854
                     /gene="nrdR"
                     /locus_tag="IWL30_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAP9705226.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            24877..26250
                     /locus_tag="IWL30_000433"
     CDS             24877..26250
                     /locus_tag="IWL30_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAP9705227.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            26250..27176
                     /gene="dnaI"
                     /locus_tag="IWL30_000434"
     CDS             26250..27176
                     /gene="dnaI"
                     /locus_tag="IWL30_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAP9705228.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            27305..27745
                     /locus_tag="IWL30_000435"
     CDS             27305..27745
                     /locus_tag="IWL30_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP9705229.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            27889..28590
                     /locus_tag="IWL30_000436"
     CDS             27889..28590
                     /locus_tag="IWL30_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAP9705230.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            28587..29708
                     /locus_tag="IWL30_000437"
     CDS             28587..29708
                     /locus_tag="IWL30_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAP9705231.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            29723..30955
                     /gene="pepT"
                     /locus_tag="IWL30_000438"
     CDS             29723..30955
                     /gene="pepT"
                     /locus_tag="IWL30_000438"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAP9705232.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            31087..31995
                     /locus_tag="IWL30_000439"
     CDS             31087..31995
                     /locus_tag="IWL30_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAP9705233.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            32030..32302
                     /locus_tag="IWL30_000440"
     CDS             32030..32302
                     /locus_tag="IWL30_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAP9705234.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            32313..33359
                     /locus_tag="IWL30_000441"
     CDS             32313..33359
                     /locus_tag="IWL30_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAP9705235.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            33610..36219
                     /gene="clpB"
                     /locus_tag="IWL30_000442"
     CDS             33610..36219
                     /gene="clpB"
                     /locus_tag="IWL30_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAP9705236.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(36370..37041)
                     /locus_tag="IWL30_000443"
     CDS             complement(36370..37041)
                     /locus_tag="IWL30_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705237.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(37034..37528)
                     /locus_tag="IWL30_000444"
     CDS             complement(37034..37528)
                     /locus_tag="IWL30_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP9705238.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(37548..38768)
                     /locus_tag="IWL30_000445"
     CDS             complement(37548..38768)
                     /locus_tag="IWL30_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9705239.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            38915..40750
                     /gene="lepA"
                     /locus_tag="IWL30_000446"
     CDS             38915..40750
                     /gene="lepA"
                     /locus_tag="IWL30_000446"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAP9705240.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            40946..41857
                     /locus_tag="IWL30_000447"
     CDS             40946..41857
                     /locus_tag="IWL30_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9705241.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            42853..43104
                     /locus_tag="IWL30_000448"
     CDS             42853..43104
                     /locus_tag="IWL30_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705242.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            43187..43459
                     /locus_tag="IWL30_000449"
     CDS             43187..43459
                     /locus_tag="IWL30_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705243.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            43549..43788
                     /locus_tag="IWL30_000450"
     CDS             43549..43788
                     /locus_tag="IWL30_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705244.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            43935..48218
                     /locus_tag="IWL30_000451"
     CDS             43935..48218
                     /locus_tag="IWL30_000451"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HAP9705245.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            48317..48682
                     /locus_tag="IWL30_000452"
     CDS             48317..48682
                     /locus_tag="IWL30_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705246.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            48684..49136
                     /locus_tag="IWL30_000453"
     CDS             48684..49136
                     /locus_tag="IWL30_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705247.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            49750..51060
                     /locus_tag="IWL30_000454"
     CDS             49750..51060
                     /locus_tag="IWL30_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAP9705248.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLV
                     RMLIDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGH
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPN
                     MLIAGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEE
                     MIICLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDME
                     ARNRVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            51083..51394
                     /locus_tag="IWL30_000455"
     CDS             51083..51394
                     /locus_tag="IWL30_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705249.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            51755..53011
                     /locus_tag="IWL30_000456"
     CDS             51755..53011
                     /locus_tag="IWL30_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9705250.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            53008..53553
                     /locus_tag="IWL30_000457"
     CDS             53008..53553
                     /locus_tag="IWL30_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705251.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            53554..54054
                     /locus_tag="IWL30_000458"
     CDS             53554..54054
                     /locus_tag="IWL30_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705252.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            54135..54691
                     /locus_tag="IWL30_000459"
                     /pseudo
     CDS             54135..54691
                     /locus_tag="IWL30_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            54711..55055
                     /locus_tag="IWL30_000460"
     CDS             54711..55055
                     /locus_tag="IWL30_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705253.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            55074..55985
                     /locus_tag="IWL30_000461"
     CDS             55074..55985
                     /locus_tag="IWL30_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9705254.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            56064..57056
                     /locus_tag="IWL30_000462"
     CDS             56064..57056
                     /locus_tag="IWL30_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9705255.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            57053..57271
                     /locus_tag="IWL30_000463"
     CDS             57053..57271
                     /locus_tag="IWL30_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705256.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            57284..57694
                     /locus_tag="IWL30_000464"
     CDS             57284..57694
                     /locus_tag="IWL30_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705257.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            57746..60274
                     /locus_tag="IWL30_000465"
     CDS             57746..60274
                     /locus_tag="IWL30_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP9705258.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            60290..62500
                     /locus_tag="IWL30_000466"
     CDS             60290..62500
                     /locus_tag="IWL30_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705259.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            62508..63599
                     /locus_tag="IWL30_000467"
     CDS             62508..63599
                     /locus_tag="IWL30_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP9705260.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            63620..64162
                     /locus_tag="IWL30_000468"
     CDS             63620..64162
                     /locus_tag="IWL30_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705261.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            64384..64614
                     /locus_tag="IWL30_000469"
     CDS             64384..64614
                     /locus_tag="IWL30_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705262.1"
                     /translation="MCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQKGYPYGPTN
                     LKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            64628..64960
                     /locus_tag="IWL30_000470"
     CDS             64628..64960
                     /locus_tag="IWL30_000470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705263.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            65005..65553
                     /locus_tag="IWL30_000471"
     CDS             65005..65553
                     /locus_tag="IWL30_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP9705264.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            65560..>65879
                     /locus_tag="IWL30_000472"
     CDS             65560..>65879
                     /locus_tag="IWL30_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705265.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCL"
BASE COUNT        22157 a        10755 c        14199 g        18768 t
ORIGIN      
        1 aatggatgga aaaggagcgg ataaaaatga acatcagaaa gttaattgct ggagtcgttt
       61 gtttagcggc agcatcaggt ttcttaacag gatgcggaag tgctagtagt acagcagaca
      121 aagaagaaaa agtcactgtc tgggcgtggg atgagacatt caatatcaaa gcagtgaatg
      181 aagcaaaaaa agtttatgag aacgaagaaa cagagataga agttgttacg atgtctcagg
      241 atgatatcgt tcagaaactg aacaccgctt tagcgagcgg gaatacagaa ggattgccca
      301 atatcgtctt gattgaagat tataggatac aaggctattt gacttcttat ccagatgcat
      361 tcgctaattt gtctgctatt gtaaaggaag atcattttgc accgtataaa tttgcggtaa
      421 acaaagtagg agataaaata tacggtgttc ctttcgattc gggcgtaaca gcaaacttct
      481 atcgtacgga tctgatggca gaagcaggtt atacagaaga ggatatggag aatctgaagt
      541 gggacgagta tattcaagta gcgcgagatg tcaaagaaaa gacgggaaag aaaatcgctg
      601 aagtcaatcc ttctgacctt ggtcgagtaa ggatgattat gcaacaggca ggagaatggt
      661 atacatctga agatggggag acagtcacga tccaagacaa tgcatcttta aagtatggtc
      721 ttgaattgtt tgccactctt ttgaaagaag atctagtcga acaaacctct gactggaatg
      781 ctggagtaac tgcgatacaa tcaggagcag tagcttctag cccaactggc gcttggtatt
      841 cttcaacgat ccaaggagca gaaaaccaat ctggaaaatg gaagattgca ccaatacctg
      901 cacttccaga aaatctgcaa aaagcgcaag cttctaacct tggcggcgct ggctggtatg
      961 tgatcaaagg agtagctgga gaagattcag cgaaagattt cctagaagca acgtttgctt
     1021 cgaatgaaga actgatgggc actcttgcga aagaaatcgg actagtatct acgatgctta
     1081 gcgcgaatga acaagaagct tatcaagaag cttctgaatt ttacagtggt caaaagatct
     1141 ttgacgattt ttcaacatgg acatctgaaa taccagaagt gaattacgga catgagacct
     1201 atgcaattga agcagtcgta gctgaagctc ttcatcgaat catcaatgga gaagagacag
     1261 acaaggtact agcagataca caaaaacaag tagaagcaca attggctaat tgatcggtgg
     1321 taaaaaagtg aatttcttcg ctttttcatc agacaaacga aaagacaaaa aagatcacgt
     1381 ctgccttaca gatattgtct ttcttgtctt ttcatgtaga taatcaatga tagaattgga
     1441 gggaaaagaa tgaagcagaa agatttattc aaaaacgggc atgctttttt aatcttgcca
     1501 gtcattttga tatcaatcat ggtttttatt ccgatggttt tggctttgat cacgtctttt
     1561 caaagtggtc cccctgtcaa tatgacattt aacgggttag gtaattatca gcggatgttg
     1621 acagatgcaa cttttaaaaa agcttttttt aatacattcc tgtatttact tgtacaagta
     1681 ccgatcatgt tgtttctagc actttttatt tccaaccttt taaatgataa aaagttgaaa
     1741 tataaagggt tgtttcgaac cgctatcttt cttccatgta tcacttcgat ggtcagttat
     1801 tctctgatca tgaaaagtct attttctcaa tctggccttg taaacaactt gttgttgggc
     1861 attggtatga ttgacacacc gattggctgg ttgactcatc caatttgggc gagagttctg
     1921 atcattcttt cgatcacttg gcgttggact ggatacaata tgattttttt cctttcaggc
     1981 atgcaaaata ttgattcgga gatttatgaa gcagcatcta ttgacggtgc ttctaaatgg
     2041 cagcagttta tcaaagtgac gatcccaaat ttaaaaccaa tcattttgtt tacttccatt
     2101 acttcgacga tcgggacatt gcagcttttt gatgaagtac aaaatattac tggcggggga
     2161 ccagcaaatg caacgaccac cctatctcaa tacatctata atttgagtta taaattcacg
     2221 cctaattttg gatatgcggc agcagtttcc tttgttattg tttcctttat tgtgatattg
     2281 tcgataatcc aaacgaaagt aggaggagaa agtcgatgaa aaatatggta gaaaaagaaa
     2341 caatcgaacg ccccaacgtc aaaaaacaag cattagaact tcgttcgaca attgggtcag
     2401 tattaaagta tacagcatta agcatcctat catttatttc cattttccct tttgtctgga
     2461 tggttttggg aatgacgaat acacccgtgg atatcacatc gggaaaaata aagattggca
     2521 gtaacttgat catcaatttt caaaatttgt tttccaatga gctgaatttt actcgttccc
     2581 tttggaattc agcgatgatc gctttggtga ccactgtgtt tgcattgctg atttcgtcaa
     2641 tggcaggata tggatttgag atttatcaaa gtaaacgaaa agacctaatt ttcaatattc
     2701 tactgctatc gatgatggtt ccttttgcgg cattgatgat ccctttatat cgaatgtttt
     2761 cacaattgaa cggaacggtt ttgggaatca acagtttctt ggttgtcatc ctgccatctg
     2821 ttagcactgc ttttttgatc ttcttctttc gacaaaatgt taaatctttt ccaaaagatc
     2881 tagtagaagc cgctcgatta gacggtctaa aagaactaag tatttttttt agagtgtata
     2941 tgccgacggc taaaaatacc tatgccgcgg ctgcaatcat cacattcatg agtagctgga
     3001 acaattattt atggccctta gtagctttgc aatctcctga taaacggacg gttccgcttg
     3061 ttttatctgc tatgggtgct tcctatacac cagactatgg aatgatcatg gctggattag
     3121 tcatcgctac tttaccaact gccatcgtat tttttgtttt gcaaaagcaa ttcgttcaag
     3181 gaatgatcgg ctctgtaaaa taaaaaaaca caataagcgc gttccttcaa agtcattttt
     3241 ccaagtaaaa agatactttt gggacgtgtg ttattttatg ttgagaaaaa ttaaatgata
     3301 acgttttaag tttgtgctcg aaaactcttt cattttaagg taaacttaaa gagtaaagga
     3361 ggaggataca tgctaaaaaa ttttaaagaa gtgattgctg ctatattgcc gatgactgtc
     3421 ctgattgtca ttttaacgtt tgtttttgca ccgttagaag gagaagacct cgcttcattt
     3481 ctggttggtg cggcaatcat gatgatcgga atgacgctct ttttgtttgg agcagattat
     3541 tctatgatgg aagtcggaga tctagtaggg aaatatatga tcaaaaagaa aagtttgacc
     3601 gttttggtca gtttgggatt tgctatcggg atcgtgatca cgattgctga accttccgtg
     3661 caagttttgg gacaacaagt atatgatata tctgacggag agattggcag agtgttgctg
     3721 attgggatcg tcagcgtcgg cactggagta tttctagcct ttgcgctgct tcgggtcgta
     3781 ttcaagctat cctattatca attgatggct ataggctaca taggcgtttt gatcgcctca
     3841 tttttcacca gttccgaatt tatgccgatt gcttttgatt cggggggagt gactactggt
     3901 ccagtaactg ttccatttat cttggccctt gccggaggga tgactagtat gatcaaacaa
     3961 aagaaaaatg aaaatgacag ttttggaatg gtcggtattg cttcgttagg gccgattctt
     4021 gccgtgatga ttttgggggt gatcttccaa tgagtttctg gcagcaagtg atagatggaa
     4081 tcgaaatcgt taccatggaa gtcattttgg cagtggcacc tatcgtaatc ctatttgttt
     4141 taatgaatat cattagtttt cgtatgcgca aaaagcgttt ctttgacatt atgaaaggtt
     4201 tcttgataac gactattggg ttgatccttt tcctgcacgg agtcaatatg gcttatgtac
     4261 cggtaggaca gcatttaggc agtgctattg cgggattgcc ttacaattat atactggttc
     4321 cccttggctt tttaatgggg ttcttagtag ggtttgctga gccggcgatt catgtaatga
     4381 tcaaacaggt tgaagaattg agcgagggga gaatccgatc gaaaatcatg ctgatggtaa
     4441 tttcaattgg gatcggcgca gcagtaggat tgtctatgtg gcgtttgctc gtcggattct
     4501 ctctttatta tttcttgatc ccaggatacg ggctagcatt tattttagga cgaaaagtgg
     4561 acaagatctt tttagcaatg gcttttgata acggtggagt agcaacgggc ccgatgtgtt
     4621 cgacatttat tttatcaatg agcgtggcaa tcgctgcaca aattgaagga agagatccga
     4681 tcattgacgg gtttggtgtg gttgcaatga ttgcattgac accaatatta tccactttag
     4741 tactaggata tatttataaa cgaaaagatg aaaaagacag aatcgaaaaa gaaagattgc
     4801 aaaaattggg ggaaagcaaa tgaaagatct tgtagcattg aatcttcaca tgattgtgac
     4861 gatcgttgac ggaggaatgg gcgaagatgt tatcgacttt tcaaaagaag caggtgcagc
     4921 aggcggaacg atcttacatg gaagaggttc tggtgtacat gattcaggga aatttttagg
     4981 tttggacatt tcgccagaaa aagatattgt gttgacatta gtacccgata gtttgacgaa
     5041 taaagtaatg gatgcgattg gtcaaggcat caacatcgat attccaggta atggtatttg
     5101 ttttagtatt gatgtagaca aagtcattgg tatcactaag attcatcaat atcgagaaat
     5161 tatcggaatg caagaacttt tagatgaaaa taacgataga gaaaagtctg aatcatagac
     5221 aagcgtttga agaggaaata aacacaaaca agaaggcgga tgagcgttat ttccgctttt
     5281 ttgtttgtat ttttctcgtg aatacttatg aaatagtaaa tttttatgaa attctactct
     5341 aagcatcaga ctttcttttc aaaatatgct attttaaaaa tgatgaaaaa agggggagaa
     5401 aaatgaattt tttagttgtt ttaaaagata ctctgattga gcgatctaca tttctatatg
     5461 ttcaattgca ggaaaataaa acattgctgc atttttcacc agagttccat ctagaggggc
     5521 taacattagg agaaatctat caagcagaag ggctgtcggc tgttttgcac ttttttaagg
     5581 tagaattgga attaccagta aaagattata tcgagctttc tcttgaatct tgggatcgag
     5641 cagtggttga actgtttcca gaaggtttaa tgatcgatac aaacgatggg aagatccatc
     5701 tgactgcagc agaactgcaa aaacaaatga gttatcaaat agatgacgaa aattcttttt
     5761 ctatttttag acgtcagcaa aagattctaa aaagcttttt aaaagtgatc agtaaaaaac
     5821 ggaatctgat caaaacgaca cagctgttga aaaaatatcc aggtctgctt cacacgtcta
     5881 ttcaatttcc tcaattgatt acgatgatcc atcgttttat acgtatgcaa cagctaccat
     5941 ctaagaagct gtttcttcca ttgacggaca catttcgagt agtcaacaga gaagataaaa
     6001 agaaagtgat tgtgattgac tttaccagaa atcggaatgt ccttcaccaa gttgcaatgg
     6061 aaaaggccaa ttaaggcttt ttctttttgg atttttttgt cataataaat attagatgat
     6121 ggaggagata atatgatgca aacatggtta gaaaggctaa tacgatttca gcagaactat
     6181 cacaaactta tgcagggaag atatgcgaaa tttgatcagt tgaatcgcgg tctattgatc
     6241 gctgcgcttg tctttagttt gctaagtcgt tggttgccct atagaatcgg acaattgttg
     6301 ttcatcgtct ttttcggatg gatgatattt cgtttcctat caaaaaaaat ctaccccaga
     6361 ctaaatgaga atcaacgtta tttgaaatat cttgatagag tcaaagaaaa atatcatcag
     6421 ataaaaaaca aaaagcaagt caaaatggat cgaaaaagtt atactttttt tgagtgcccg
     6481 aattgtcatc aaaagcagcg tgcacctaaa ggaaaaggac gaatacgtgt gacatgcaaa
     6541 acatgtggta taaaattcga aacgaatgtt taagggagaa aagaatgaaa ttagtattat
     6601 tacgtcatgg cgaaagcgaa gcaaattttg aaaattactg gacaggatgg ctagatgtcg
     6661 ccttaacaga aaaaggaaaa gaacaagcag cagttgccgg tcaaaaaatg cttgaagccg
     6721 gacttcaatt cgatgccgtt tatacctctg ttttgaaacg tgcgattctt accggacaag
     6781 tcgcactaga agaaatgggt cagttatggc ttcctataat aaaaacatgg cgtctaaatg
     6841 aacggcatta tggggatctt gtgggtaaaa acaaagataa gatgaaagaa atatacggaa
     6901 aagatcaagt aaaaacatgg cgcagaggat atacggaagt tccgccgtta accgcagaaa
     6961 atagatttga ccgcagatat gatcacttgg atccgcattt gatcccatac ggagaaagtc
     7021 tggaaatgac agtgaatcgg atcattccct tatggcaaga tcatctagca ccaaaactaa
     7081 aagatcggca agatttacta gttgtgggtc atggtaatag catccgagca ctgacaaaat
     7141 atttagaaga tgttcctgaa gatcaaatgg atacgatcga tatcccaaat gcacaaccaa
     7201 tccagtatac attagcagat gatttgacaa ttctggacaa aaaaatactt taaagaacac
     7261 ctttatctaa aagtgagaga tagaaaaaag aagcaaagga atggaacaaa aggaacatga
     7321 aaaattatat atgggacttt gatggtacat tgtttgatac gtatcccgca atggtagatg
     7381 gtgcatggca agcactaaaa gattttggta tcagtatgga caaaaaagaa atctacttca
     7441 aaatgaaaaa gtattctact agttatttga tcaatgaatc aaacttgaat gccagagagt
     7501 ttaatgaatt atttcaccga tatgaaaaag aaagtacgga agtttcccgt ccatttccag
     7561 aaacgaaaca agtactggaa atgctaaaag acaacggcgg tcgtcatttc atcctgaccc
     7621 accgtttgac agaatcaaca tggggcctgt taaaagaaca tcgattggca catctgattg
     7681 aggaagtagt agggatcgat caggactttc ctcgtaaacc tgatccagca tctttaaatt
     7741 atttgatcga tacttttcat ttagaaagaa cagacacgat gatgatcggc gatcgtcgtt
     7801 tagatatcga agcagggaaa aatgcaggag tcgctacttg tctttacgac atcgatcatt
     7861 ttctgggtga gatcccggca gattatgtgg taggaaatct gaatgaaatc cttaccttgc
     7921 agagcatcat taaaaagtag tatccccttt caaaggagat atgttataat tttcataaag
     7981 gagtgataaa attatatgga aatcagaaga caaaaagaag ttgtagaagc atttcattat
     8041 gatatgagat tgccgaacca agaagtagaa acagatttga aagtaggctt ctcgccttta
     8101 aaagaaacga tcgaaaacta tccgaaagaa catagcatcg taggagcacg cttagagttc
     8161 cgtttagtgt ttgatacata tgttttatct ggaagtgtga gtcagatcaa tcatatcctc
     8221 aatcggaaaa ttgaaggtca aaatgatgtc acacaagaag aagtggacga gctagtagca
     8281 ccgttattcg atatcgtacg ccgaatgtct tatgaggtga cagagatcgc cttagatcag
     8341 cctggggtaa aattgaattt tcaatcagaa aaacaagaat agacagtaaa aacatacctg
     8401 cgatagatta aagctatcac aggtatgttt ttttattact aattgtaagc aaaaagaatg
     8461 tcatttcgat aaaaatcgtc taaaatataa aagtcactga aaaaaatttt ttcaggagga
     8521 tggttgaaga atcattctcg ggaaccttta cttccttcga tggacttgtc attaataata
     8581 tgaggaggag aaaaatgaca acactcatca cagttttagt ggttctagtt tttgcggcat
     8641 tattatatgt gttctatcgt atgcaaaagc gccatacaaa attttcaaca cgcgtcttta
     8701 cagcattagg agttggtatc gtctttggcg gtctgcttca attgatcttt ggtgtagacg
     8761 acaaagtcgt tactcaatca atggaatgga tcgggattgt aggtcaagga tatgtatcat
     8821 tcttgcaaat gctagttatg cctttagtgt ttatttcgat cattggtgca ttcacaaaaa
     8881 tgaaagtttc tgaaaagcta gggaaaatta gtgcgactgt attgacttct ttacttggaa
     8941 caacagctat tgcagcattg atcggaattg gttcaacaat gctgttcgga ctccaaggag
     9001 ctaaattcac acaaggagct gcagaaacag cacgaatctc tgaactggca acacgtcagg
     9061 aaacagttga aaacttatcg attccacagc agattttatc ctttatccct aaaaacgttt
     9121 ttgctgattt agcaggtagt cgtgcaacaa gtacgattgg agtcgttatt ttcgcggcat
     9181 ttgtcggaat cgcttatcta ggcgtgaaaa gaaaagcgcc aaaagaagca gcattctttt
     9241 cagatttagt cgacagtctg tataaaatca ttatgcggat cgtgacgctt gttttgcgtt
     9301 tgacacctta tggagtattt gctttgatga caaacgtttt ggcaacaagt gattttgcag
     9361 ctatcgtcaa tttaggtaaa tttgtcgttg cttcttacgt agcattgctt gctgttttga
     9421 ttgtccactc actgattttg attggtgcaa aagtcaatcc aatcaactat ttcaaaaaag
     9481 cctttccggt attgagcttt gcgtttactt cacgttcaag tgctggagct ttgccattga
     9541 atatcgagac tcagacaaaa gctttaggag tagatgatgc aacagctaat ttctcagcaa
     9601 gttttggttt gtctattgga caaaacggct gtgctggcgt ttatccagct atgttggcaa
     9661 cgatcgtcgc accagcagct ggagtgaatg tctttagttt ggaatttatt ttgatgcttg
     9721 tggcaattgt aacaatcagt tcttttggtg tagctggagt cggtgggggc gcaacatttg
     9781 cttcactgat cgttttaggg gcaatgaact tgccagtagc aatcgttggg ttagttatct
     9841 ctgttgaacc attaatcgat atggcacgta cagcagtaaa cgtcagcggt agcatggtag
     9901 ctggtatcgt gacaagtgca cgcatcaaag atttagatcg cgatgtcatc aatgatgatt
     9961 ctaaagtgat cgatgtgaat gcatagtcaa aaagaggttg tgatagaagt ggtcagcttt
    10021 aagaaataag aagaagtttc ctaaaaatag tttctcaaat ttttgtgaaa tttcggctta
    10081 tttccgagaa gctacttctg aaacaccgtg aataagaggt tgtgaaaaag ctgccctgca
    10141 tcaagtaata agaacagcca aacaaaaata gttcctcata ttttacgttt ggttgagctt
    10201 attaccgcag atgcaagctt ttgaacaccg tttaagaaag aggtcgtgag cctgccgttt
    10261 cactctgagt cacaaagagc aatttcaaaa aatagcttct catgtttgtt gaatattgtg
    10321 tcttgtgacc gaggagttgt caggagaaca ccgtttacca gaggttgtga cttgttttta
    10381 gtcacgacct ctttttttat ttattccata taaatacaca ttatttattc cgaaaagtga
    10441 aagcgcaaat aacaaaaaca accaacttat ttattattaa agaaaagcgt atttaagtag
    10501 gacgattctg ttgagagctt tcgtagattt tcccattttc ctatggtaaa ctgacaaagt
    10561 tgagaatgtt agaacagatc gtttaggagg aaacagatgt ttacgaatga aataaaaatc
    10621 atgctttatg tggacgatgt agaaaaaaat gcaacgtttt ggagagcagt tggctttgcg
    10681 gaaaaagatc gtcaagaaat ggatgggacg cttataattg aagtaggagt tgaggacagt
    10741 caaacctcat ttctgttata tgatcgagca tttgttgagt cccattcacc tggcacagct
    10801 actagtgccc catctttgat gttttacagt aaagatatct ttggactata taaaaaaatg
    10861 cagacgcaat cagtcacatt aggtgatatg gtacagcttg gagaagaata tgtctttaac
    10921 tttgctgatc ccgatggcaa ctactttgcg gttacaggta aataatagaa ataaagactt
    10981 tgtttttctg tgcgaaacag gaaagcgaag tctttttaat tcaaataata atattatgta
    11041 aacaaaaaac tttccatgcg taattataca caagcaaata aatcgtcaaa tgcgaacgcc
    11101 ttctttatac tgtttaggaa cgaaaagaag gaaggaagaa atacatggat tatcaagtac
    11161 tgctttatta caattataca aaaatcgaag aaccagagga atttgccaaa aatcatttag
    11221 ccttttgcaa atctttggat cttaaaggtc gtattctagt agctgaagaa ggaataaatg
    11281 gtactgtgtc tggaaccgtt gaaaacacga ctgcctatat gaaagcaatg aaagctgatc
    11341 ctcgatttgc tgatacgatg ttcaagatcg atccaagcga aggacatgcc tttaagaaga
    11401 tgtttgtaag ataccgtcca gagcttgtaa gtttaaagct agaagaggac attgatccaa
    11461 accaagtgac aggtgcctat ctttcaccgc aagaatttaa agaagctatt ttagatgaac
    11521 agacagtggt gattgatgca cggaatgatt atgaatatga cttgggacat tttcgagggg
    11581 ctgtacgccc agatatacgc agttttcgag agcttccaga ctggatccgc gagcacaaag
    11641 aagaatttat ggaaaaacgg gtagtgacct actgtactgg tggcattcgt tgcgagaagt
    11701 tttctggttg gcttgtccgt gaaggattca aagatgtcgg acagctgcat ggcggtattg
    11761 ctacttatgg taaggatcct gaagtccaag gagaattatg ggacggcaag atgtatgtgt
    11821 ttgatgaacg gatcgctgtc gatatcaacc aagtagataa acaaatcgtc ggtcgcgact
    11881 ggtttgacgg aacaccatgt gaacgatata tcaattgtag caatcctgag tgcaatcgtc
    11941 agattcttgc ttccattgaa aatgaagaaa aacatttagg agcatgtagc atagagtgtg
    12001 cgaaacatcc gcgcaatcgc tatgttcaag accatggaat ctctccagaa gaagcagaac
    12061 tgcggtttac agctttaatt aaatagaaaa caatagtttt gattgtcgac catttccaat
    12121 atgcgctata ctagcgttga ggatggtgat ccacatgaaa tcattgattt atgctattgg
    12181 ggatgtccat ggcaaacatg agctttttga aaaacttatt cactattatg atccaaagat
    12241 ccatcagctt gttttattag gggatctcaa tgatcgaggt cctcgtacaa aagaatgttt
    12301 tttattagga aagcaattag tcgaagaaac aggagccatt tacctccggg gaaaccatga
    12361 agaatatttt ttgcaatttc tgcaatcacc ggaggactgg tatgcgcctt atatgagaaa
    12421 tggtggaaaa gaaacgtttg aaagcctgat tcacccaggg gcatcggagg aatactcccc
    12481 aactgaaatc tcgatgatga tccaatcgag atattcagaa ctgattaaat ttttagtcca
    12541 gcgccccttg tattatgaat gggaaaatta tctttttgtc catgccggtg tggatttgac
    12601 aaagaaagac tggcgtaaga caagtctaag agactttatt tggataaggg aaccatttca
    12661 tgaagggaaa aacaatacag gtaagacaat tgtatttgga catacgatta caccaatgct
    12721 ccatggagat atgcagacaa cagacctctg gattcaggat cataaaatcg gaatggacgg
    12781 tggggctgta ttcggagggt cattgcatgg ggttgttttt gacaaggaag gtattcttca
    12841 agatattgaa tatcagaaca acgaaggacc ttggcatgga atctgactaa tggaaagaat
    12901 gaaggagctg tgactttgtt gcggctcttt ttatttttat agagaatatt gtttctttca
    12961 gtagtttaac ccaggataaa aagtatgata gaataaagaa gcaattttta gcagaaaaga
    13021 ggcattttaa tgacggaaaa tttgaaccag acgatcattc agcttgtaca aaaagaactt
    13081 tcagactatc gtcctaagca gttgactact gtacttaatt tgttaaatga aggaaacact
    13141 gttcctttta tcgcgcgtta ccgaaaagaa atgactggaa gtcttgatga agtccagatt
    13201 cgtgaaatcg aagagcggta tgcctatctt gaaaacttag aaaaacgtaa aactgaagtg
    13261 attcggctaa tcgatgaaca agggaaactg actcccgagt tagaagcaga aatcacccaa
    13321 gccgtgaaaa tgcaacaagt ggaagatttg tatcgtccat acaaacaaaa acgacggaca
    13381 aaagcaacga ttgctaaaga aaaaggactt gaaccactcg cgaagtggct gatgcagctt
    13441 acagatggag aggtgcaatc tgaagcagaa aaatatattg acaaagaaaa agaagtctct
    13501 tctgccgaag aagcgttgca cggtgctcat gagattattg ctgaacaagt cagcgataat
    13561 gcaaaatttc ggacttggat tcggtcttat acgtataaca aaggtatgta tgtcagcaat
    13621 gtcaaggatg agcaagcaga tgaaaaaggc gtatatgaaa tgtattatga ctttgcagaa
    13681 cccgtccata aaatggtttc tcatcgaata ttagctacta atcgtggaga aaaagaagat
    13741 attctgaaag tattctttca agtagatgaa gcagcaattt tagcttactt ggatcgccaa
    13801 atagtcaaaa atcctgcctc tccttcttct tcattcgtca gagaagccta tcaggatagt
    13861 tataaacgct ttattcagcc agcaatcgag cgagaactgc gcaatgaatt gactgaaaag
    13921 gcagatgagc aagcaatcgc tattttcggc gaaaacctcc gtaatctttt gttgcaaccg
    13981 ccactaaaag gtaaagttgt cctgggattt gatccggctt atcgtactgg atgtaaacta
    14041 gcagtcgtag atgcgacagg gaaagtttta gcaatcgaag ttatttatcc tcataaacct
    14101 gcagcgcaag caaaaagaga agctgctgga ccagctttta tccaattaat taataaatat
    14161 caagtagata tggtagctat cggaaatggt acagccagtc gggaatctga actttttgtg
    14221 gctgagcagt tgaaatccgc cgaccataaa acctattacg ctatcgtcaa cgaagcaggc
    14281 gcttctgttt actccgctag cgaaattgca cgaaaagaat ttccgcatct acaagttgaa
    14341 gaaagaagtg ctgtttctat tgcccgtcgc ctgcaagatc ctttagctga attagtcaaa
    14401 atcgatccta aagctgtcgg agtaggccag taccaacatg acgtttctca aaaacgtttg
    14461 gctgaacaat tggattttgt tgtggaaaca gcggttaacc aagtaggtgt ggatgtgaat
    14521 acagcaagcc cgcagctctt gcagcatata tcaggattga ataaaacaac tgcccaaaac
    14581 attgtcattt atcgtgaaga aaacggagaa ttcaccgcac gaactcaatt gaaaaaagtg
    14641 ccacgcttag gaccaaaagc atatgaacaa gcaatcggat tcttgcgagt ccctggtggg
    14701 aaaaacattt tggacaacac aggtatccat cccgaaagtt attctattgc aaaagaaata
    14761 ttaacttcag tccaactttc agaaaaagag cttggtacag aagaagcagt agaaaaattg
    14821 acacgattat ccgtagagaa actggctgaa agcctatcag tcggagaaga aacgttagcc
    14881 gatattttag ctgggctgac acaacctgga cgagatatgc gtgatgagat gccagcgccc
    14941 ttattacgta cagatgtatt gagtatggaa gacttgaaac cagggatgga attgactgga
    15001 acggtccgta atgtcattga cttcggcgca tttgttgata tcggtgtgaa gcaagacgga
    15061 cttgtccata tatcgaaact cagcaaaaaa ttcgtcaagc atcctacaga tgtcgtgtca
    15121 gtaggagata tcgtaacagt ttggatagaa caagtcgata cgaaaaaagg cagaatcagt
    15181 ttgacgatgc tttcccctta tgaagagtaa caatgaggag taacaaaaat tgacacaaga
    15241 agaactgcag cagttagtag aagagatatc attaaaaagc ttccagcttc ctttcaagca
    15301 ccaagcaatg ttcaatccac gcttacgtac aactggcggg cgctatcatt taaacgatca
    15361 tcatttagat tttaatgcga agctatttga agagtcagac gaagagacga ttgcaggaat
    15421 catcaaacat gaactttgcc attatcatct tcatctagct ggaagagggt atcagcataa
    15481 agatatggat ttcaagtatc ttttgaaaaa aacaggaggg ttgcgctatg caccagcctt
    15541 gacgaagaaa cagataaaga tggagtatta tcgatgccaa gattgttctg ccacgatctg
    15601 ccgcaagaga agaatcgatg tacgcaaata tcgttgtgga agatgtcatg gagaaatcaa
    15661 atggattgag acaaaaagag aagactttaa tgaagcacaa aaaatcagat gaaaggatag
    15721 aaggcctttg gaaaaaagtg agcagaggaa tctaaaagac tggaatcagc tattaaattt
    15781 tttagccgca cctgaagagg gaaattttga ggaactgacg gcacctacac ttattttagc
    15841 tggaaactgt cttcctatct tagctgataa agcagcacaa atgtatttaa atggccaagt
    15901 agatcaattg tttttggtcg gaggggtggg gcatgcgact agaatccttt atgagaattt
    15961 tgaaaagcaa ggatttcatt ttgaagatgg catgagtgaa agtgaaatct gtcgtcagta
    16021 tctaaaagaa gtctatgatt tgcccgataa ggcttttctg atagaatcga agtctacaaa
    16081 ttctggagag aacgccactt tttcactgga gattcttcat tccttaggag ctgtaccaga
    16141 aaaaatctta ttgatgaatg atccgacact gcaacgcagg actcgagcaa cttttgagaa
    16201 agtatggcaa aatgaacaaa ctattttcgc gaatgctgtg ccatttgtgc cggaaatcct
    16261 tcacttttct gaagagatca tctttacagc aaaagaactg aatcatcaat ggcctaagga
    16321 atatttccat gcgctagtat taggagaaat ggaacgtctc catgacgatg agaacggcta
    16381 tggtccgaaa ggaaaagatt ttattcctca cattgatata tcagaggaag tttggtcttc
    16441 ttacaccaga attaaagagt caataaaaac ggatttttct cgtacttgac cattttagca
    16501 agcgaatcct cctttccaaa taaatttggt tgaaattttc aatcgtttat aagataatga
    16561 ggagcgtgtt gaatgtaata gctaaaagaa tggacgctag ataagagaag aggattcaaa
    16621 tggaaaatca aaataaattg tatcattttg ttggtatcaa aggttcagga atgagctcct
    16681 tggctcttgt tttacatgaa caaggtctaa atgtccaagg atcagacatt gaaaaatatt
    16741 tctttacaca aagagattta gaaaaagcaa atatcacgat tctgccattt aacgcagata
    16801 atgtaaaacc agggatgacg atcattgcag gaaatgcatt tcctgattca catgaagaaa
    16861 tccagcgagc aaaagaatta ggtttggaag tgattcgcta tcacgacttt attggtcatt
    16921 ttatccaaaa ttatacgagt atcgctgtaa caggctctca cggaaaaaca agtacgactg
    16981 gactgctctc tcatgtattg agtggtgtcc gtccaacaag ttatctaatt ggagatggaa
    17041 caggacatgg tgatccgcaa gcggaattct tttcatttga ggcctgtgaa tatcgccgtc
    17101 atttccttgc ctattcacca gactatgcaa tcatgaccaa tatcgatttt gatcatccgg
    17161 attattatac aagtatcgac gatgtattta cagctttcca aacaatggct ggacaagtga
    17221 aaaaagctat ttttgcgtat ggtgacgatg cttatttacg caaattaaaa gcaaatgtgc
    17281 cgatttatta ttatggtgtc acagaaaacg atgatatcca agcacgaaac atcgaacgca
    17341 caacttctgg ttctgctttt gatgtctatc atggggatga atttgttggg cacttcactg
    17401 taccagcatt cggtaaacac aatatcttaa atgctttagg tgttattgca gtagcttatt
    17461 tcgaaaaact ggatctgaaa gaagttgcag aagaaatgct gactttccca ggagtaaaac
    17521 gtcgattcag tgaaaagatc gtagcagaca tgaccgtagt cgatgattat gcgcatcacc
    17581 ctgcagagat caaagcaacg attgatggcg cacgccaaaa atatccagat aaagaaataa
    17641 ttgcagtttt ccaaccccat acgtttacgc gtacgattgc attgatggat gaatttgcag
    17701 aagcacttga tttagctgat aaagtttact tatgcgatat ttttggttcg gctcgggaag
    17761 aacaaggaaa cgttaagatt gaagaccttg gagcgaaaat caaaaaaggt ggagaagtca
    17821 tcaaagaaaa taatgtatct ccattgttag attatcatga tgcagtagta atcttcatgg
    17881 gtgctggaga tgttcaaaaa tttgaacaag cgtatgaaaa acttttaagc agcacgacga
    17941 aaaatgtact gtaatacaat tactgcagtg tccttataaa aatagaaaga gggggagtgg
    18001 caaaaatctt gccaagcccc cttttttgca acttatgata aacggtgttc aaaagcttgc
    18061 atctgcggta ataagcccaa tcaaatcaaa aacatgagaa gctgtttttt cttggctgtt
    18121 cttattattt gatgcaggac agctttttca cgacctctgt aaactatagg aatctgttaa
    18181 atcaagggag cagaacttgc cttttacgct acttttggta caataatgta tgattaaaaa
    18241 taaactggag ggtttctatc ttgaacatag gtaagccgag aaaactagga aaaacaataa
    18301 atgaaatcga ggaaggcgac tcgttatcct taacagaatc tattgaagat aaccagcttt
    18361 tgctgtattt gggattgacg aatgatgcaa atcctttgta tatccaacat gactttgcac
    18421 aaaaaacaga atatcaacag ccaatcgttc catcagtgat gctgatgggg atcatcacaa
    18481 gtgcaatttc aaagcatctt cctggaccag gatcacatgt tgtcaatttt tctgtcaatt
    18541 ttattgaacc ggttttccat tatgaaacac tgacttttca gtttgaggtc ataaaagtag
    18601 ataagatgaa ggatgtggtg acgatttctg tagaagcgga gaatacgaac gaagatcgtg
    18661 tgttggatgc tgtcgttatg gtccaaccgc cacaagttcc tgaattggaa atagcaatcg
    18721 aaagtgaagg tgaattgaat gaataatcca agtacatcaa tgtcaggtat caatcgtttt
    18781 tatgcaaaag tttatggatt tttaggtatg ggacttgccc ttagtgcagt cacttcttac
    18841 ttggctcttt gggtatttcc tttgcaatta gcaactttta tcaataattt tccactaggc
    18901 tttgtgggtc tgtggatcgt tgaactgatt ttagtaatgg ttctaggcgt aaaagcacaa
    18961 aagaatccga gcttagcaat cggtggattt attgcctact ctcttttgaa tggcataaca
    19021 ttagcggtta ctcttgcata ctacgatatt ggaacagtaa cgcaagcttt cttgacagct
    19081 actttgatgt tcatggggat gtctgttgtc ggagtaacaa caaaaagaga tcttagcggg
    19141 gttggaagag caggacaggc agctttaatc ggagtgatca ttgcactagt cttgaacatc
    19201 ttcattttga gaagtagcgt agttgagctg ttcatctccc tactgttagt cgtcatcttt
    19261 gcagggatta cagcttatga taaccaaagc atccgtaata tctatgtcca gacaaacgga
    19321 acagcacata gtggaattgc tgtattcatg gcattgaagc tgtatttgga ctttatcaac
    19381 ttgttcttgg cgtttctgag aatctttgga cggaacaact aaaaaagaaa aaaattcatc
    19441 agttggggct ataccgaatt atttggtatg gtcccgcttt ttcttttcgc attccgcagg
    19501 attctttgtt agaatgaata agaactttac agtaaggagt taagaagatg acaaaaaata
    19561 aattattact cgttgatggg aacagtgtgg cttttcgcgc attctttgcg cttcacaatt
    19621 ctcttgaacg tttcaaaaat agaaacggtc ttcatacaaa tgcaatctat gcattcaata
    19681 cgatgtttga aaatgttatg caaaaagagc aaccgactca tgttctggtt gcgttcgatg
    19741 caggaaaaac gacttttcgg acagaaatgt atgcggagta caaaggtgga agatccaaaa
    19801 cacctggcga attcaaagaa caaatgccat atatacgtga tttgttgaca ggactagggg
    19861 tacagtatta cgaattgccc aattatgaag cagatgatat tatcggtact ttggcagaaa
    19921 aagtagacaa agatcaattt gatgtcgtcg tgctttcagg tgatcgggat ttgactcaat
    19981 tagctagtaa agaagtaaaa gtggatatca ctgtaaaagg tgtcagcgat atcgaaagct
    20041 atacaccaga acatgtagcg gaaaaatatg atgggttgac gccaaaacag attattgaca
    20101 tgaagggact agccggagat gcctctgaca atatccccgg tgtgactaaa atcggtgaaa
    20161 aaacagctat caagctattg aaacaatatg gctcagtcga aggaatttat gaacatatcg
    20221 atgaaatgaa gcaaagcaaa atgaaagaaa atctgataaa tgacaaagag caggctttct
    20281 tatcgaaaaa attggcgacc atcaatactt cagcgcctgt ggatgtctct atcgactcat
    20341 tgaagtatga agggaaaaat ctcgataaac tcgttccgtt ttataaagaa atggatttca
    20401 atcagttcct ttctaaattg aatatcgtag aagaagaagt aaaaatggat gatattttgt
    20461 ttgaagtcgt tcatgaattc aaagaggaaa tgtttactac ggatatggcg ttgtacgtgg
    20521 aaatgatggg cgacaattat catacagagg aaatcgtcgg agtagcgtgg gggacagaaa
    20581 agaaaattta tgtcacgaac gatctatctg tattcgaaaa ccgagcgttc cattcttgga
    20641 tcactgatcc gactcggctg aaaaaagtat atgatgcgaa acgaacttat gttgctttaa
    20701 accgctatac tggaaaaacg aaaggaatcg attttgatgt cttgctaggt gcataccttc
    20761 tcgatacgaa tgaaaaaagc acagatatcg aaggaatagc tgctcattac ggatacaacg
    20821 atatccaatc agatgaagct gtatacggca aaggggcgaa aaaaggttta ccagaagaag
    20881 aggaactctt ttttgctcat ttagcgcgaa aagtagctgc aatcaatgcg ttgacaccaa
    20941 aattgagcca agaactggca gaaaaaaacc aagaagatct tttccggaaa atggaactgc
    21001 cattgtctat cattttggca gagatggaaa taagcgggat caaagtagat gcgactcgct
    21061 tacaagaaat gaagggcgag ttctctgctc gcttaagaga gatcgaacaa aaaatctacg
    21121 aagaagccgg cgaagaattc aatttgaatt caccaaagca attaggagtg atcttatttg
    21181 aaaaaatggg attacccgtt atcaaaaaaa caaaaactgg atattccaca gccgtagatg
    21241 tactggaaca gcttcgtgaa caagcaccaa tcgtagagga tatcctcacc tatcgtcaaa
    21301 ttgcaaaaat tcaatctacc tatgtcgagg gactgctgaa agtgatccaa tcagatggaa
    21361 aagtgcatac ccgttatgtc cagacattga cccaaacagg acgattaagc tctgttgatc
    21421 ctaatttgca gaacatcccg attcgattag atgaagggag aaagatacgt caggcatttg
    21481 ttcctagaga aaaagactgg ctgatttatt cttctgacta ttcacaaatc gagttacgcg
    21541 tacttgcgca tatctcaaat gatgaacatt tgaaagctgc cttcctagaa ggtcaagata
    21601 tccatgccag cacagcgatg cgtgtatttg gaatcgaaaa ggcagaggat gtcacaccaa
    21661 atatgcgtag gcaggcaaaa gcagtgaatt ttggtatcgt gtatggcatc tctgattacg
    21721 gactttcaca aaatctagga atcagccgaa aagcagcaca gcaatacatc gatacgtact
    21781 ttgaaaaata cccaggtgtc aaagaatata tggaaagaat cgtacgcgaa gcaaaagacc
    21841 aaggctacgt cgaaacgctg tatcaccgcc gtcgatatct gccagacatc aattcaagaa
    21901 attacaatat ccgttctttt gcggaacgaa cagctatcaa tacaccaatc caaggaagtg
    21961 ctgctgatat tttgaaaatc gcgatgatcg aattggataa acgattaaaa gaaacaggtc
    22021 ttcaagctac gatgctgtta caagttcatg atgaacttgt cttcgaggta ccggaaaaag
    22081 aacttgaatc attagataaa ttggtgaagg aagttatgga acaagctgtt tctcttcatg
    22141 taccactgat cactgacagc agttggggaa aaacgtggta cgaagcaaaa taaacattgt
    22201 ttaagacaga gcaatataat gaaggtgaaa agaaaaaatg ccagaattac cagaagtaga
    22261 aaccgttcgt aaaggattgg aacggttagt agtaggaaaa acgattcaaa aagttcaggt
    22321 cttgtggccc aagatcatcg agcaaccaga gacacccatc tttgaggctt ctcttgttgg
    22381 cgaaaccatc caatccattg gacgtagagg gaagttttta atttttcatc tcgatcattg
    22441 tgaactgatc tcacacttgc gaatggaagg aaaatatcaa tttacgaaag aaaatacacc
    22501 aatcgataaa catacgcatg tacttttctt ttttgaggat ggaagccaac ttcgctacaa
    22561 cgatgtacga aaatttggtc gtatgacaat cgtcgaaaaa ggagcaagtg caacttaccg
    22621 aggaatcatg aaattgggac ctgagccttt gccagatagc ttcttattag cagatttcgc
    22681 aaatggactg aaaaagtccc acaaagcaat caagccattg ctgctagacc aacgtctagt
    22741 gactggtcta ggtaatattt atgtagatga agcattatgg gaagcgaaga tccatccaga
    22801 acaaccggcg aacacactgc gccccaaaga ggtagaacaa ttacgtctat cgatcattga
    22861 tgttttggat cgtgcaatag aagccggagg taccacgatt cgcagttatt tgaatgcatt
    22921 aggagaatca ggcggatttc aagtagcact gcatgtctat cagcaaactg gcaaaccatg
    22981 tatacgttgt ggaacgccga tcgtgaaaac aaaagtggca caaaggggga cgcactattg
    23041 tccgaattgt caaaggctaa aaatcgcaaa gtaggatttg ttttaggttt gacgggtgga
    23101 atagctaccg gtaaaagtac ggcagccaaa gtctttcagt ctcatggatt tcctttgatc
    23161 gatggcgatg tgattgccag agaaactgtt gagcctggaa cgcttgctct gaggaaaatt
    23221 gtatcagtgt ttggccaaga ggtattgcag cctgatgggc aactagatcg agggaaactt
    23281 gggatgattg tttttccatc aaaggaaaaa cggcaaaaac ttgatcagct attagatccg
    23341 tttatccgaa aagcgataaa agatcaaatc gcctctctaa gttccaatca tcctttagta
    23401 attgtagata tcccattgct ctacgaaggt cattatgacc actatatgga tgcagtagct
    23461 gtcgtttata ctacgccaga aactcagctt cagcgtttga tgaaacgtaa tcaactgacg
    23521 ctagaacaag cgcagcagag aattagtagt caacttccaa tcgaagaaaa aaagcagcgt
    23581 gcggatattt tgttcgacaa caatggaaca aaagaaaaac ttgtgggaca aatcgaaaat
    23641 tggctttttg ctaatcgctt tctttgagta tctgtgtcaa ttttgctatc ctgagtatat
    23701 agaaagtgag ggatagcgat gggatcatta aaaaatcaaa aggataaagt cgtaggtaac
    23761 gcaaaagaaa aaatcggcga gatgctcaat aaagaaaatc taatcgatga aggtcgtacg
    23821 caatcagaaa cagcagaaga aaaagaaaag cagtaccaag caagaatcga tgaaatagaa
    23881 gaccagcagg cagaagaaga agcgaagcgg caagatttga ctcaagaacg aaaaaatccg
    23941 acacctgatc gtagtaaaac accgctccca gaaagtgacc acattcccga tgagcaaaaa
    24001 atcgaggaac agcgaatgaa acactataca ggaatcgaag agaagttata aaatagtaaa
    24061 aaatcgagat gtgacttgaa gcagatgaga aggtgttcat ctgcttcaag ttttttgtgc
    24121 tagatgacta aatcagcttt tgtgtaagtt atttctcagt aatcaaggtg atcagtattg
    24181 gaaaattgaa atttagtaag gacaggggac acaattacgc atgaaagtag aaaaaattgt
    24241 tcttatatcc gtgttttttg ggtaggacag tcgcgtttgt gatttgtttc gtgttataat
    24301 acaactagtt gtaaagaaag acacatcctt tatataaaat gcgatgcaag aggtgagaca
    24361 aaatgagatg tcccagatgt caacataata attcacgagt aatcgacagc cgtcaagcag
    24421 atgatggacg tgcgatccgt cgccgtcgcg aatgtgagaa ttgttcgttc cgattcacta
    24481 catttgaacg aattgaagct gctccgttat tagtaatcaa gaaaaacggg gaacgcgaag
    24541 agttcaatcg cgataaaatt ttaagaggac tcatccgttc agcagaaaaa agacctgtag
    24601 ctatggaaca aatggaacaa atcgtcaacc atgtcgaaaa ccgcgtacgc agtctcggag
    24661 aaaacgaagt atcgacgaat ctaattggtg aatatgtgat ggaagattta gttgatttag
    24721 acgaaatcgc ttatattcgt tttgcaagtg tttatcgcca gttcaaagat atgtctgttt
    24781 tcttaaaaga gttgcaagat atcgtggata aagcaaaaga aaataataaa gagaataata
    24841 aagaaaacaa ttaagaaaca aaaaggaggc atttccttgg aaaatgcttg gaaagagctt
    24901 caaccaaaaa atatttacca aggaagaaaa gcggctccgc tgagagaaga cagcaagaac
    24961 gccttgctgt atctttatca gccactaatc ggcggagaag ccttgagtat ctatttcacc
    25021 ctgttatcag aaatctctca agaatctggc aaaggtcctg aagggatgca tgcagatctg
    25081 cttagtagtc taggctgcgg tatcccgcaa ttttatgatg caagaaaaaa gctagaagga
    25141 atcggcttac tggatgttta ttttaaagaa gatagtgatc tgggacctcg ttttatttat
    25201 gagctgttag aaccactcat tcctaaagca tttttcaaag actctgttct ggcttttctt
    25261 cttctggaaa aagttggtga acgacgattc cagaagctta cccaattctt tgaacctaaa
    25321 aggcatacag cagagggata tcaaaaaatc acaaaaaaat ttttagatgt ttatcgcttc
    25381 aatgaagcag tgtacgctgc taatcaagaa aacatcgaat cgctgaataa atcttttgaa
    25441 gaatctgaat cagtagcatt acttgaagca gattcgacat tcgattggca gtttttgaat
    25501 gattggctgg caaaaaaaca tattgcaagt cttgatgatc caatcatcaa acaagtcgac
    25561 atgtatcatc agctttacgg tttcgatgaa ttgacacttg gcgaactgat cagtcaatct
    25621 tatgacttta gtgaacaaaa agtatctgaa aaagaactgc aaaaagccat tttggcaatg
    25681 cagagacgtc cggtgaagca aacaaaagaa acagttgcgg tttctgaaaa cagtaatgaa
    25741 ccctcgtcaa acgaacattt gacaaacgca gcgcttcagc tgataaaaga agcacagagc
    25801 attccaccga tgaaatatct ggaagcaatc aagtcagaaa aaggcggata tgtttctaaa
    25861 caagaaaact ggttactgca agatcttgtt tctcaatcag gtctttccag tagcgtgatc
    25921 aatgttttgc tgaattatgt actagtgatc aagaataatg ctagcttaaa tgccagttat
    25981 gtaaatacag tggcaaatga atgggcacaa aagaaaatcg ccacagcaga agaagcgatc
    26041 cagcacattc ggcaaataag taatcaagca aaacaatcaa aacaaaagaa gcaaacaaat
    26101 tatcaaactg gcaagcgaaa tgttcgacgg gaaaagcttc ctgattgggt caaccagcca
    26161 aaagatgaaa aacagatcag cagcgagaaa aaagcagaga ttgaccgccg ttttaaagaa
    26221 tacctggcaa aaaaggaggg tgatatctag tggaagacgt aggcaaagaa ttgaagagga
    26281 tcatcagcaa acgcaattat caagaaaagt atgctgaaat gatcgctgaa gtcttaaatg
    26341 atgcggatgt tcaagctttt ctaagcgcac accaagaaga attgacacaa gcggatattg
    26401 aaaaaagtta cgctaaattg tatgaattcg tgcaagaaaa gcgtaaatat caaaaaaacg
    26461 atccaacgat gatcgctccc ggctatgagc cgaagctgac gttgaatttt cattatgtgg
    26521 atgtcactta tgtaccaacg gaagcattgt tagcacggca aagacaagaa gaaatcagaa
    26581 gccgagtaaa agcaatggat atgccaaaag atattcaatc ggcttctttt gaaaactttg
    26641 aacgtacaga tggacgagga tatgcgagct tagaagccct agattttgta gagaaatata
    26701 ctgctgatcc aaaagggtat cataaaggac tgtatctagt aggaagtttt ggtattggaa
    26761 aaacatattt attaggagca acagcgagag aattagcgat tgctggattc accacgacgc
    26821 tagtccattt tccgacattt gccgttgaga tgaaacaggc aatcggaaaa gatcaagtgg
    26881 cagaaaagtt agatgcagtg aaacgttcac ctattttgat gatcgatgac attggtgcag
    26941 atgcgatgag tagttggata cgagacgaag tattcggtgt tatccttcaa tatcggatgc
    27001 aggaacagtt accaaccttc ttctcatcga attttacaat ggatgaattg gaacagcatt
    27061 tgtccgtcac tcaacgtgga gatgaagagc cgctaaaagc gaaacggata atggaacgca
    27121 tacgttattt gacaaaagaa atcgaaatga ctggtcgcaa ccgacgcaat ggttgaacag
    27181 ttgacaagtc caactgacat ttgacctagt agtaaaagca ggtcagcgaa tcattatatg
    27241 gacagaaatg accggagaca atcaccgcaa caagtaatgc agaaacttaa acggaggatt
    27301 ttttatgagg gctatttttt ctagtatatc tgtagttggt ctattttttc ttttagcagg
    27361 ctgcggtagc gcagatagca acacaggttc tgttgattcg attgatgcgg gtcaaacagt
    27421 aaaaacaagc agtacgaaaa cggagagaca aagcatattt gtcggaacga ttgaaagtat
    27481 cactcaagta gaagcagaag aagaaacgct tcagttgaaa ctcgtcgatg tcatggccaa
    27541 agaagatcca gcgaatatgg gaacgtcatt cagcaatgac ggagtgatct taaatgctac
    27601 tgctgaaaaa ataagcgtgg acataaatga attgaaaatc ggtgacaaag tccaatttac
    27661 attagttgag atgccgatca tgacgatgtc tatcccaccg caagttcctg gagattcgat
    27721 tattgatgtt tcattgaaca aataaccaat aacatttttt caaaaataag aaaatgttgt
    27781 tttatagaac gaaaaggtat attttttaag gagatatggg aattttttta ttcctagtct
    27841 ccttatttta tttggaaaat gctataatta tctagaagga gtgaagctat gaaccattta
    27901 gatttatctc agcgcttagc cgcagtgggt gactttgtcc ctgaatccgc tcgtttagca
    27961 gacattggat cagatcacgc ttacttaccc gttgcattga tattaagagg aaaaatcgaa
    28021 tatgcagttg ctggtgaagt cgttcaaggt ccttatgaat cagcacgtaa gcaagtcaaa
    28081 aaagatggat tagaagaccg tatcaccgta cgccttgcaa atggtctaga tgctatccag
    28141 ccacaagatg gtattacagc tgtcacgatt gcgggtatgg gaggttcact gatccgagat
    28201 atcctagaag ctggcaagtt gaatcaacgc ttaagcggac aagaacgttt gatccttcag
    28261 ccaaacatcg gagagcgtac attacgatct tggctgcaag aaaatcagta taaaatccaa
    28321 tcagaaaaaa tacttgaaga aaataaaaaa atatatgaaa tcattgtagc cgaaaaagaa
    28381 caaaaaccag ttacttacaa tgaaatggag ctgatgttcg ggccatttct gcttgaagag
    28441 aaaagtgaga tcttcaaatc aaaatggaca agagagcttc aacaaagaaa agctatccgt
    28501 gaacagctca aacaagcagc taatcaagag gcacgtcaaa aacaaatcga gcaggaaatc
    28561 caaatgattg aggaggtttt aaagccatga caatcagcgg acgcactttc atccaaaagt
    28621 ttgaaagcta ttgtcctcag tggctagctg aagaaggaga cccagtcgga ctgcatatcg
    28681 gaacattgga taagccaatc caacgagtaa tgatgacatt agacgttcgt ccagaagtgg
    28741 tcgaagaagc aatcaagaaa aaaatcgatt tgctgattgc taaacatcct ccaatctttc
    28801 gtcctgtaaa acggctggtt actgatcagc cacaagaaaa aatgtatgca gatttgttga
    28861 aacatgatat tgccgtctat gcagcacaca cgaacatgga tattatttgg gatggactga
    28921 atgactggtt ttgtgaactt ctcggtattg aagtggagag ttatttagta aagactcatg
    28981 agattcatta taaaaaactg gccgtttatg tacctgtgga tcatgcacag aaaatgcgtg
    29041 aagtattagc tgcagctggt gctggtacac aaggtgatta taccggtaca agttttacct
    29101 cgattggaca cggtcgattt actccggaag ctggtgcaca accagcgatt gggaaagtag
    29161 ggaagacaga acaagttcaa gaggcaaaag ttgaggttat tttgccggaa actatcgaaa
    29221 aacaagtgat tcaagccatg cggtcagctc atccttatga ggaacctgct tatgatcttt
    29281 tcgctataga tgaaccagtc gaaatgtttg ggttaggacg agtaggtgaa ttgccgcaag
    29341 aaatttctat agaggcattt gtagaacaag taaaagaagc gttccaacta gatggactga
    29401 gaatagtcca gccaaaaaat gcaaaatcct ctgtgaagcg cattgcgatt tgcggtggaa
    29461 gcggtgaaaa attttatccg caagcaatcg cccaaagagc agatgtctat atcacgggag
    29521 atatctatta tcatacggct catgatatgc aaagcgctgg gttgattgca attgatcccg
    29581 gtcattatat tgaatcgtta tgcaagcaac gatttatcga gaaatttgaa tcttggaaac
    29641 aagaagaaaa ttgggatatt aactttttcg tttctgaaac aaatacaaac ccatttcaat
    29701 ttaattaaaa ggagagaata ctatgtacga aaatcttttg ccacgttttt tacgatatgt
    29761 caaaacagaa acacgctccg atgcaacaag caaaacgact ccatcgactc agacacaagt
    29821 tgcttttgcg caagaattga aaaaagaatt agaagagtta ggcttagctg atgttttcta
    29881 taatgaagat aacggattcg ttatgggaac attaccaagc aatgttgatc atgaagtgcg
    29941 ttccattgga tttatttccc acatggatac agcagatttt aatgcagtag gagtcaatcc
    30001 acaaatcatt gaaaactatg atggcgagtc tacgatcgta ctagatgcgg aagaacgcta
    30061 tacattgaat acaaaagatt ttccaaattt gaaaaattat gaaggccaca cattgatcac
    30121 aacagatggc agtacattac tgggagcaga tgacaaatca gggattgcgg aaatcatgac
    30181 tgcaatggag atcttgatca ataatccttc tatccctcac ggtaccatca aagtggcttt
    30241 tggtcctgat gaagaaatcg gtgtcggagc ggataaattc gatgttgagc aattcgatgt
    30301 agattttgct tatacagtcg atggtggtcc tttaggtgag ttgcagtatg aaacgtttag
    30361 tgctgctcaa gcaaatatta cgatccaagg gaaaaatgta catccaggga cggcaaaaga
    30421 tacaatgatc aatgctctac agttagcaat cgattttcat aatcagcttc cagccgatga
    30481 agttccagaa aaaacggaag gatacgaagg tttcttccat ttgatggcat tggatgggaa
    30541 tcctgaagaa gcttctatga gttatatcat tcgcgatcat gatcgtgaaa aattcgaagc
    30601 acgcaaagca caaatcacac atatccaaga agagttgaat cagcgatttg atcaagaacg
    30661 aatcaaagta gatatgtatg atcaatatta caacatgaaa gaaatcattg aaaaagatat
    30721 gagcatcgtg gaattagcga aacaagcaat gatcgagctt ggtatctctc cagtgatcga
    30781 accagttcgt ggaggaacag atggttcaaa aatctcttac atgggtattc cgacgccaaa
    30841 tctatttgcc ggaggagaaa atatgcacgg acgattcgaa tttgtttctt tacaagtaat
    30901 ggaaaaagca acagacgtga ttgtaaaaat tgcagaattg aacacaaaga gttaaaatcg
    30961 aaaaagaaca gcttatgaca aggcgttcgt cccaatgatg gaatgtcatc tgtctaaggc
    31021 taaagggcag gacaatggaa ttggttgtcc cgccctcttt tttgaagagt aaataggagg
    31081 taaagaatgg aaaagctgct agttttttat aacaaaagtt cagggaaaga tgagggtgag
    31141 aaattagcac gctggtttca agattatgta aaagaaaagc agccagaatt atatgtagaa
    31201 ctagctgaaa ctggcccatc gattgatgat gcggaattag tcaagaaagc agaagccata
    31261 gaagctgata ctttagtaat tataggtgga gacggcacga ttcatcatat cgtccaagcg
    31321 ttccaaaaaa cattatctca ataccatgtc ggattacttc caggtggaac tgtcaacaac
    31381 cttgcacgtg ttcttgagat tcccctcaaa aaagaagaag ctgctgacgt gatcctggaa
    31441 ggcaatgtac ggaaaataga ctatggtcaa gtcaatgatg aagtcattat ctctacactg
    31501 acaatcggaa tcttagcaga tacagcagtt tggatcagtc aagaagaaaa acagcgttac
    31561 ggctcgtgga tctttatcaa gaagtttttt aagttattgc tgaagaagaa aaaatatcat
    31621 ttggatttag aaacagacga ggagcattgg aaaggaaaat cccaactgtt gacagtcacg
    31681 atgtctaact ccgtgggcgg ttttacaaat tttgacgact cagcgaccct agatgatgga
    31741 aaatttcatg taacgatcgt accttcctta gatattttcc gatttatttt ttatttgcct
    31801 agaatcatga gaggcaaatt ctatacgatc cccggcatta cttatttcac agccaaacgc
    31861 atgaaaatcc aagcgcagga aaaatctgtg ggcactcgaa cggatggaga cacgacagat
    31921 gatcttccag ttgaattatc tgttgtatca aaaggattaa cagtttattc accaaagaaa
    31981 gaagaaaaag cttagaaata gtagttaaca gaaagttgga agaagagtaa tggatgtatt
    32041 gttacacaat cctttgtggt tttacctttt cattgtgaat ttttacttat tttgtctgat
    32101 ggggtatgat aaacatcagg ctagaactag cggctggcgt gttcctgaga ataatttgat
    32161 atttgtcagc ctgttaggcg gtggtttggg aggattgatc tctcgaaaag ttttccatca
    32221 taaaactcgg aaaaagaagt ttactatagg attcgctgca ggtgtacttg ttgatttgat
    32281 tctcatttat tttttccatt aaggagcagg agatgtcgtc taaaggaacg aaaactaaaa
    32341 tcattctgaa tctgcttttg ctgattgtga tttttgccat tatttattat ttaatcaatc
    32401 aatcatttgc agatatcttt gccgaattgc tgtctaccag tgttgttgtt ttggcagcgg
    32461 ctgttatatt agggacagtt taccaaatcg ctgaagggcg ctcaatcaaa gaaattgctg
    32521 aacctttcgc gccgaatttt acaacgaaag atgggttctt gacctctagc tatattgcgt
    32581 tttaccgaat cgtttcgttt ggtacaggaa cgctgatctc ggagatttat ttttataaca
    32641 aaaaaggaat gaagctatcc cagggagcag gggtgacagc actccatatg attatgtaca
    32701 aactggcagt gatcacttat gcattagctg gcttggtcat ccaattctct cttttttatt
    32761 ctaatggtcc atcaatgatc tggtttatca tcgctggtat ccttttgacg atcgtgattg
    32821 ttgccttttt attggcgatg tccgtcagct tgaatgttca aatatttttt gtcagcttat
    32881 cgaataagtt atttaaaagt gagcgtttgc ggaaatgggt ggatacatgc aatcagcaga
    32941 tctactcttt aagggaaacg gtccaaacca ttatccaaga caggactgcg ctattgcgga
    33001 tttatctatg gaatatgtta aaacttgtat tttggtatgt acttccttat ttgattctga
    33061 ttgaaaacca tcctaaaata gattttctac tgaccatttc gtttatcagc tttgcagtag
    33121 tgctttcagg ggttattcct acgcctgctg gtattggttc ctttgaattt gtctatttgt
    33181 tattattcag accagttgtt ggaacagttg acgcagtttc ttcgctttta ctgtatcgtt
    33241 tcagttcctt tatcttgcca tttctctatg gatttgtgta tgtgattgct gaacggcgaa
    33301 gagtgatcaa agaagaaata atagaagtaa aaaaagagaa taaagaaaat caagggtagg
    33361 tcttttataa gatctaccct ttttatcaaa tcaaagaaaa acgttatgga taagataatt
    33421 ttacgtttgt ttggctcttt ttttatatac atacaatcaa ttttgttttt ttaagattta
    33481 tataagtcga gaaaaaaagt caaaaaacta tgtgtatatt ttgaccaaaa ttgaccaaag
    33541 cgatataata gtttttgtaa agatcaaaga aataagtgat cttaccttta attaggaggg
    33601 tatacctgta tgaatattga gaaaatgact acgacgcttc aagaagcgat tgctgaagct
    33661 caacagattg cggtcactcg aaaacatcaa gatatcgaca ttgcccatgt atggaaaatc
    33721 tttttacaat ctaatcattt tggtcgaaat ttctatacgg atgccggatt ggatgtcgaa
    33781 tcttttgaac atgaaattga tcgtctactg gatgagtatc cagttgtttc tggtggaaac
    33841 gtccaatatg gacaaaacct cagccagaat ttctttcgat tattgaatga agcagaccaa
    33901 atacgtgaaa gcttcggcga cgagtttctt tcaacggaag tcgttatttt agcgttgatg
    33961 aaattaaaaa attatccgtt aactgtttat ttgaataaaa acggattatc tgaaaaagaa
    34021 ttgcgtaaaa acatcgaaga gatgagagga ggagaaagag tgacctcaca aaaccaagaa
    34081 gaacaataca aagcattaga aaaatacggc gttgatttag tacagcaagt caaaagcgga
    34141 aaaatggatc caatcatcgg tcgtgatgaa gaaatccgtg atgtcatccg tattctttca
    34201 cgaaaaacaa aaaataatcc tgttctgata ggagaacccg gtgtagggaa aacggcaatc
    34261 gttgaagggc ttgctcaacg tatcgtacgc aaagatgtgc ctgaaaattt aaaagataaa
    34321 acgatttttt cattagatat gggtgcattg atcgctggag caaaattccg tggtgaattt
    34381 gaagaaagac taaaagcagt cttgaaagaa gtcaaaaaaa gtgatggacg tatcctcttg
    34441 ttcatcgatg aaatccacaa tatcgtaggg gcaggaaaaa cagaaggaag tatggatgcc
    34501 ggaaatctgt tgaaaccaat gcttgctcgc ggtgaattac atttgatcgg tgccacaact
    34561 ttagatgagt accgtcaata tatggaaaaa gacaaagcgt tggaacgtcg ctttcaaaag
    34621 gtattagtca aagaaccaac agtggaagat acgatcagta ttctgcgtgg attaaaagaa
    34681 cgttttgaga tccaccatgg tgttaacatc catgacaacg cattagtagc agcagctact
    34741 ttatctgacc gttatattac tgatcggttc ttaccggaca aagcaatcga tttgatcgat
    34801 gaagccagtg caacgattcg ggtagaaatg aactctatgc caacggaatt agatcaagtt
    34861 actagacgtt tgatgcagtt ggaaatcgaa gaagctgcac tgaaaaaaga atcagatgat
    34921 gcaagtaaaa aacgcttgaa aaatttgcaa gaagaattag ctgaacttcg tgaagaagca
    34981 aatgcgatga agatgcagtg ggaaacagaa aaagaagaag tcaattctgt ttcagcaaaa
    35041 cgtgctgaaa tcgataaagc aaaacacgaa ttagaagatg cagaaaataa ctatgattta
    35101 gaacgcgctg ctgtattgcg tcatggtacg atacctcaat tagaaaaaga attgaaggaa
    35161 ttagaagcta aagcaaaaga cagcgagatt aagatggttc aagagtccgt taccgaaaat
    35221 gagattgctc aagtcgttgg cagattaacg ggtatccccg taacgaaact tgtagaaggt
    35281 gaacgagaaa aactaattaa attgaatgaa acacttcata aacgagtgat tggtcaagat
    35341 gaagcagttg atgcggtcag tgacgcggta attcgttcac gggctggatt gcaagaccca
    35401 aatcgtcctc taggttcttt ccttttccta ggaccaactg gtgtcggaaa aaccgaatta
    35461 gcgaaagcgt tagctgagaa cttgtttgac tcagaagatc atatggtacg gatcgatatg
    35521 agtgaatata tggaaaaaca tgctgtgtct cgtctagtcg gagctcctcc aggctatgta
    35581 ggttacgaag aaggtggtca attaacggaa gctgtcagac gaaatcctta tacaatcgtc
    35641 ttgttggatg aaatcgaaaa agcgcatccc gatgtcttca atatcttgtt acaagtccta
    35701 gatgacggac gcttgactga ttcaaaaggg cgagtagtag atttcaaaaa tacggttctg
    35761 atcatgacca gcaatatcgg ttcacagtta ctgctggaag gcgtgactgc tgatggaacg
    35821 attccagaag cagtggctga acaagtcaac acgctattac gtggaaactt caaaccagaa
    35881 ttcttgaacc gaatcgacga cacgatttta tttacaccat tgagcctaga caacgtgaaa
    35941 ggaatcgttg acaaaatggt ggcgcaattg gcacaacgtc tggaacatca agaaatcctg
    36001 ctgacaatca gtgatgaagc caaaacgtgg attgctgaaa atgcttatga accagcatat
    36061 ggtgcaagac cattgaaacg atttatcact aaggaagtag aaacaccact agctaaagaa
    36121 atcgttgcag gacatgttat gcctaaatca aaagtaacga ttactttact tgatggacaa
    36181 cttcatttca aaacagaaga gctagaagaa atcgtctaat cttccattga gactataaaa
    36241 gaaaaaaggg gtctgacaca actagtgtca gacctccttt tttcaaataa acagtgttcc
    36301 agaagtagtt tcttcgaaaa ataagccgaa atttcttgaa gctgcccgct tctgtcacaa
    36361 cctcttttat taggtatttt tcactttttc taatttataa ctttcaaagt aacgctcatc
    36421 ttttttactt tgagaccatg caaacaatcc cattaaggca gcacctatcg tatttgtcag
    36481 caagtcgccc atcgtatcca tcaatgctgc tcgtccgaca aacagtgtgc catcagaagc
    36541 agcaaaacgt tgaagattca tgcctaagaa ctggtcgcat aagaattccc agaactccca
    36601 aaacacgccg caaagtccag caaacgcaaa gccgaataag aggaacaacc aaggactggt
    36661 ttttgatatc tcagcatctt tcatcaagta accaatcaat ccatagccaa gagctgtcag
    36721 gaccattgga ctcaccgcat gtaagatttt gtcccaaaaa gagatgatac tgatcaaatg
    36781 catccctgtg cctaaaaaga cagagatgaa caagaaaaac cagtaaaaca aaacgattgc
    36841 tgctggtatt ttaagtttga aaactttcaa aatgacttgt ggcatgaaaa tcaatgcaat
    36901 ccctaataaa gattccacga taaatgtgat tccttgagaa gtcgaatatt tttgtgtgat
    36961 aaattcataa aaattatagc agacaacgaa tacaccaaaa gcaagtaaag ctatttgcac
    37021 ctttttgatt tctttaatca ctgaaagtcc ctccatctaa aaatcttttt acttctgaaa
    37081 gcaactcaat ttctgaagaa accacttttc cgttcaattt gatcaaacct attgtatata
    37141 agttcaggta gtgaaattga ttttcagctg tttcttgtag tgcagcgatt tttttcgggt
    37201 gggttccgcc ttgctgtcga gtatcagtat ataagccgat cactgggatg cctttcgcat
    37261 aagccacacc gatttccgat gctaccccgg catcgatcgt cagcccatct agtaaagcaa
    37321 tcattaaatc actagccaac actttttctg tgtcagcaag agcaatcatt cgactgtctg
    37381 cataagcagt tttgtcattg attgcttcat tttcttgagg aagatagatc gaaagctcag
    37441 aagagagctc tctgatttct ttaactaact gttggttata taacaaatca cttttagcaa
    37501 acattggtgc ggcaaaataa attttcatct aaacatcatc ctttttatca aaaataatcg
    37561 ctaaagtaag cttccatgtt tggaatgata cgctctagtg acttcagtgt ttcttcatct
    37621 gtttctaacc gttcgattct tgccaaataa gaagcacggc gataattcat catcaagcaa
    37681 gtcaatgttt ggatatccag tcgaacggat tttcctatag gttcatttaa gacacggact
    37741 tgtccctgtt catcccatgt taaagcgaag acaccattat tccaaggagc gacaggatct
    37801 tcaatgatga aatgaaaagc atcagctgtt ccaacaaacg gaaaccgttg aaggaattct
    37861 tttacatcaa cgatccgagc cataaaatat ggttcgatct gctctttgat ctgactatct
    37921 tcaatcaaaa atgctaaagg ctcattttta taaatatctc catgtaccca gtagatcatg
    37981 gaaaaatgtg cggaaataaa gttccataag ccatttcgag cttcttgatc aagatagaac
    38041 atttctttta cgtgaaatat ttcttcagct acccagtaga acagaacacc tcttggttca
    38101 tggttgctat cgtagtagac agcggctgtg cgctcatctt cattttcgaa acgccaatac
    38161 tcttcccaat gaaaatcact gcggaataaa gcaccatggt tttgttgggc aaatttgtca
    38221 taaacggaaa atacgtctgg atgatcaact ggtaatcgct caaccatacc acttactggg
    38281 atttgtttag gtagctgtgt atctctgatt ttaaaggaaa gtttatcaga catgatttcc
    38341 catcctttac gccgatagta ggggatactg taaggataca aataagagat ccattgctta
    38401 ttcttgcgca tattatccaa tgcaacgata attagatctt gcatcaatcc atggttagca
    38461 tattctggat aagttccaac tccagtcaca ccacccattt tataacgtgc accgtgaata
    38521 ttgacttcac aaggatagat agcgatttga gagatcagtt tattttcgtg gaaccatccg
    38581 aacactttcg atacttccaa aatcggtttt ttggaacgga taaaagctcg tttattctca
    38641 aacccgcttt cctcgatgtc tgcttcagtc acttggaaca cataggaaag caattcattg
    38701 aattgatcga tatgttcttg agtgaccggt tttaattcaa gattttctct aaaatctttt
    38761 tgatccattt tttcatctcc atttattagc ttgataacag tatagcaaaa ttctacaaaa
    38821 aaaacttcta tagctgacta attaaaaact ctttaaatta ctgcttttcg ttggtataat
    38881 ggaaagtgct gattatatga aaagaggaga cgctatggat ataaagaaaa tgaaagaacg
    38941 ccaagagcag attcgtaatt tttcgatcat cgctcatatc gaccatggga aatctacatt
    39001 ggccgatcgt attttagaaa tgacgcacac tgtgacgtct cgcgaaatgc aagatcagct
    39061 gttagactcg atggatttgg aacgtgaacg tggaatcacg attaaattaa atgctgtaga
    39121 actcaattac acagcgaaaa atggagaaac atatatcttt cacttgattg acaccccagg
    39181 gcacgtcgac ttcacctatg aagtctctcg aagccttgct gcctgtgagg gtgccgtttt
    39241 agtggtcgat gcagcacaag gaatcgaagc acaaacattg gccaatgttt atttagcgtt
    39301 agacaatgat ttggagatcc tgccagtcat caataaaatc gacctacccg cagcagatcc
    39361 cgaacgtgtc cgtcaagaaa tcgaagacgt aatcggaatc gatgctagtg aagctgtatt
    39421 agccagtgcc aaatcaggga ttgggattga agatattctc gagcagatcg tggaatatgt
    39481 tcctgcacca agtggagact tagatgcacc gctaaaagct ttgatctttg actctattta
    39541 tgatagttac cgcggtgttg ttctaaatgt ccggatcacg gacggtatgg tcaaacccgg
    39601 agacaaaatc aaactgatga gtaatggcaa aacatttgat gttacagagg tcggggtctt
    39661 ctcgccaaaa gcagttgcta gagacttttt gatggtcggt gatgtcggtt atatcacagc
    39721 aagtatcaaa actgtccaag atacacgagt aggggataca gtaacgttag cagacaatcc
    39781 tgccgctgaa gctttaccag gttaccgaaa aatgaatccg atggtttatt gtggtcttta
    39841 tccaattgat acttctcgtt acaacgattt gcgggaagct ttagaaaaac tgcaattgaa
    39901 tgatgctgct ttacaattcg aaccagaaac ctctcaagca ttaggttttg ggttccgctg
    39961 tggattcctc ggacttcttc atatggatgt cgtccaagaa cgcttggaac gggaattcaa
    40021 tctagaattg atcacgactg caccatccgt tatttaccat gttaataaaa cggatggtac
    40081 gactgctaca gtagataacc cggcagattt tcctgaacct gtaacgattc aggatgtaga
    40141 agaaccattc gtcaaagcac agatcatggt cccaaatgat tttgtcggtg cagtaatgga
    40201 actttctcaa cgaaaacggg gagaattcat tacgatggat tatttagatg attaccgagt
    40261 aaatgtcgtc tataatattc cgctttctga aattgtcttt gactttttcg acaaattgaa
    40321 atcaagcacg aaaggctatg cctcattgga ttatgaaatg tctggttatc aaaaaagcaa
    40381 gttagtgaag atggacatct tgctgaatgg agaaaaagtc gatgcattaa gctttatcgt
    40441 tcaccgagac tttgcctatg aacgtggaaa agcaatcgtt gaaaaactga aaaaactaat
    40501 cccacgccaa caatttgagg tgccaatcca agcagctatt ggacaaaaaa tcgttgcccg
    40561 ttcagacatc aaagccttgc gtaaaaacgt cttggctaaa tgttatggtg gagatgtttc
    40621 tcgtaaacgt aaactcttag agaaacaaaa agaagggaag aagcggatga aacaaatcgg
    40681 ctccgtcgaa gttccgcaag aagccttcat ggccgttctg aaaatggatg agcaagatag
    40741 tgtgaaataa cttataatca atcgaaaaac ctattaaata aacctttttg agtagatgca
    40801 acactgagga ttatctgtat aaaagatttt aaactgtcat ttggttatca cggcgatact
    40861 aaaattaaca aaattggaat atttttcttg aagattttta gctgtcagta tatgatgaaa
    40921 gcatctcata ggaggtgctt ttttcatggt tgataattca agtggaaggg ttcttaaatc
    40981 tctaagaaaa gagaaaaaac tatctcaaaa aaagttagct gatttagcag gtatttcaca
    41041 aagtacttta gttaaatatg aaaaagggtc aagaaaaatc cctaaagacg tagataatac
    41101 tctaagcaag attctgaata tagaaactct aataaaagat gaggaagata agattgagat
    41161 tctcatcggt aaactaattg catacagaga tatgaataag ttacttaaca aagagttagc
    41221 tgataatata ggaattagtg aagtattact cagttatgtg ttaaatagaa agagaaatcc
    41281 gtcaaaagaa atgcaaaaaa aaatcgatat ttttctatta agtaatgaaa aagaaatttt
    41341 gaaggagatt aatcgtgata gtgagatatt tagtttatca aaagacgata aaattgttat
    41401 gggaaaaaga atacgagaag tccgaaagaa ccgggaagag acgcttgaaa aatttggtaa
    41461 aaactttacc atctatacag gcaaaaatgt tataagtaga tgggaaaaag gtattaatat
    41521 tccagatatt gaaaaactaa tgaatattgc ctatcttggt aaggtcactg ttccttacct
    41581 tatgtacgga gaagattata agaatatact tccaaaagat gaaagagtta gcgattttaa
    41641 aaagataaat tcttttagta tgggattaag gatgaggaaa attaggaaag attattatct
    41701 agaaagggag gagtttggaa agcttttttc tccttcaatt tcaaaatggt ccatagatag
    41761 gtatgagaat ggtagagata tacccaatac taatcgaatt atccaatatg cttatatagg
    41821 aaatctttct ttagaatttt tgatttacgg gatataaaaa tagtttacgt cgatacttat
    41881 taagttatcg acgtttttta ttatagaaaa aatttataaa aggaggtttc ttgatatgat
    41941 aagaaacaaa aagggtttgt tgaaagttgc tttactggca gcttttttct aagtaacttt
    42001 ttgacaagct caatggcaac acaagcggaa gaagtaatac aagaactaac tgttatgaat
    42061 aacagctaga taaaatgggc tatgtatgtt atccaacaat ttagtaatca ctagaagcct
    42121 agcgaaagtt cgcttgataa gttatagatg agtattgatt tgtagtgttt ccaaatcaaa
    42181 agggtacaga gccacatgaa atatgttgtc ctctgcagcg tcaggatggg aatccatacc
    42241 atggagattc ggagatctgt gagggaatac ttacgtggtt aatatgtcag ttgtgttgga
    42301 cgtagtaatg gtcatagtgg gtagattgac tgaattctta tatgagaaaa tgaaaccaac
    42361 aagaagatag ggttatttat acagaattgg ggatcttttc tggaaaagta atcggataat
    42421 gcgagattcg tacggtcttt tttaaatttc tctaaaaaaa acggcatcga atgggttggg
    42481 tgattgataa gcaatgaatc atagactgat atagaataaa aaatgattct atttatcttt
    42541 ttacatctat cgttttttca agtagccgca aattatatca gcattcaaca atttttgtat
    42601 acaagttcta gtaaaaggat ctgaagaaaa agttggttgt tgtgaagata aaaaaagaat
    42661 caggttttac tgagtttttc ttcaaaattt gtgttattag gttaggacaa agataagaag
    42721 aagtatgaag atttaaaaaa ggaatgagga aaagatagta atttccgatt attggagttt
    42781 ttttatttat aaagaagaag cgaataagtt gtgattccta gatttcgcgc gtgattaatt
    42841 aatgagcaaa aaatgcggaa agttttagtg tttaatacac gaggacaaag tttcttaggt
    42901 attgaaggca cacgtgtaac ggttgatggg tggtgtgatt gtttcgaggt tggaaaacat
    42961 gttgaactta tggcatttac tgagctaaaa gataagaaca acgtggaatt tttcgagagt
    43021 gatattggtt gggacgatca tgaagaagtt tatggaaaat ttattttgcg gaagggaaat
    43081 tcttatacga atggggaagc ataagtagcg atttgttaga gatcattgat aggattgaaa
    43141 ttgtcgggaa tatttatgag aatctagagt tatttgaggt gatagaatga gtgatggttt
    43201 tgaacaagta tcaataatta aaaatcaagt tcgggagatt cttagaaaaa aaggctattt
    43261 ggtagatagt tattttgaag gagattacga aacatgggta ggtgtctatg ctcgtccaga
    43321 gaataaacct acctatttag atcctacgac gtcagaagat gcctatttac aaaataggta
    43381 tcgagtagat ggttttaagc aagactttgc ggagtggttt gaatgggaaa ttgaaaacgg
    43441 agaagtaaaa gaagaataag agtacttgtt gtatcaaaat aagccagtag aacaaccaaa
    43501 ccagtagcta gtaatgacaa taatactgag gaatcggatc attatggtat gattgatatt
    43561 tctcttccag aagatcttaa gccagatcct gtaacaattg gcaatagtgt catgattttt
    43621 gattcattag atgaagctat gtcatatggt tggaaagaaa ttaaggatga aggtagtaat
    43681 tggtatggca tgagatttgg tgatggtgaa gttgccaaaa aagctggtat tccaattggg
    43741 aaatggacag ttgatttcca cgaagcaggt tctttttttg ttctgtaatt atagtgggag
    43801 ggtaaaatca ttgatgaaat gtgttcagat aagagcgcca gataaaaata gaagagacag
    43861 ttcacgtcgt tatcactttt ggtaacggcg ttttgtttta ttatataaaa ttctcaggaa
    43921 aaggaggaat tcttgtgtat gcaaaaacga aaatgaattg gttgaaagca ctcgcattgg
    43981 tggctttttc tcaattctgt ttcttggtgg gaactgctca agcacatgca gaagaaacga
    44041 cggctataca acctacgatg gaagccattg cccaagtcgc ttctgaagtg acagaagtcc
    44101 aaccagcaga agccggggtc ccagctggtg aagccactgt tgaacaggcc cctgtgacag
    44161 aagcgccaac ggtcaattcc aaaacgactc tcgatcagac gattggggaa acacaacacc
    44221 cagggaatag tcctggtact tcacaaccag ttgaagaaac tgcctcaatt acaacccctg
    44281 cttcagaaac gtccactcta gaaggagaaa aagctgccga agcaacgcct tctgtgcagc
    44341 cagagtcttc tgagaaacaa gcggaagaaa agaaacgaat agaagaaaca gtggaacaac
    44401 cagcggctac tgctgacaaa gaaggaaaaa atgaggaaaa cgttgaaaaa acaatctctt
    44461 caaccgcagc tagtcaaaat caaccaacga aaccaaacaa agaatctacg gctagtattg
    44521 aaaatcaaaa gaaaaaagag cagaacattc caaaaacagg tacacctaca ctgttagtaa
    44581 cagtaccaac caatcgttcg gttcgtgttc gccgtgcaac caatgcagct gttttaggtg
    44641 gtcggaaaat tgattggggc gaaaatattg gtgttacttc tagtccgaac aatctaaact
    44701 tcactttaaa ttatatggtt cgttttgaga ttaatggaca agtggcgttt tgtattgagc
    44761 ctggtgtctc agccgcagaa ggtcaagttt atacagctac tgctttgaaa aactatttga
    44821 aaaattcaaa tgtacggaaa aaaacaagct tgatctctta tttaggatat gtcacaaata
    44881 aagacaaatc cgatgaacaa tacattgcgg cacaatttat gatttgggaa gctttgggca
    44941 caaaaatccc aatcaaaaat atccgtgttg attatgatcg tcgaaaagca gaaattcaac
    45001 gtttgattaa taactttggt gaaaaagcaa cttttaataa tcaaacacat acggtaaaaa
    45061 ttggcgaaac gttgaaagta aagaataaaa atgatttttt gaaatatgtg aaagacattc
    45121 aaaaaccaaa aggtgtcaat gtatctattc acggcaatga agtatggatt acagtcacaa
    45181 aagaggcacc ggataaagca aaaattcaat tgaataaaat taaagttcaa ggtgcgccat
    45241 tagcctatcg tcgtccagga gctcaaacaa ttggtgtgtt acatccaaca gaacctaatg
    45301 cgtctgttat taatttacaa attctaaaac aaggaaacat tcgtgtgttt aaagaagatg
    45361 cggaaactgg tgtgaaagca caaggtgcag cttctttaga aaatgcggtc tatggtttgt
    45421 atcaagcaga tgggaaaaag attaaagaaa ttaccttgaa aaaaattaac ggcaaagtac
    45481 aggcagaaat taaagatctt gatcctggca aatatgtttt aaaagaaatc aaagcgccaa
    45541 aaggttatgt gctttctgat gaagcaattg aaattgttgt tgaacctgga aaaactgtca
    45601 atgcgaccgc aaaagatcaa gtgatcaaag gaaccattga tttagtgaaa gttgctaata
    45661 aagatttagt cgattcaacg aatccagata acaaaccaaa attagctggc attgaaatca
    45721 ctttgacgtc aaaaacaact ggcaaagtgg tgaaaaccgt tgtgacagac aatgatggtt
    45781 atgctacttt tggtaaaaat accgttgtct ttgatacgta cattctttct gaaaccaaag
    45841 gaaaagaagg gtataaacta tttgaaccac ttgaagtcac aatttctgaa aatgggcaaa
    45901 ctttccatta tgttttagaa gataaagtaa ttgaacaacg tcttaaagtg gtgaaagtgg
    45961 atcaagaaac aggaaaagtg attccacttg ctaatacaga attcaagatt tttgatactt
    46021 tagccaatcg ttatgtcaca atggagattc caaatgatac agaaacaacg gatatcttta
    46081 aaaccaatga aaaaggcttt ttcctaacta acggtacact tacttatgga aaagatcgct
    46141 atcgtttgga agaaatcaaa gcccccgtaa attacgtttt gaatcaaaac ccattagtgt
    46201 tctcgattac caatgataca gaaccgatca aagtgatcca ttttgcgaac cgtcaagcac
    46261 ggaaaagtgt aaaattattc aagtatgaag aatggaacaa gaagaaaacg ccgctcgcag
    46321 gtgtgaactt taccttgtat aacggtaaaa atcaagcaat tggtgaatat accactgatc
    46381 aaaacgggga aattttagtg aaaggtctag ttgccgatga ctattacttc ttggaaaatg
    46441 ccccattaga aagtttccaa ccaaatacag ataaacaagc attcacggtt gcattcaaac
    46501 aaaatgcgcc agatgatggg gaaacgttag tggtttctgt acgtaattac ttgattccac
    46561 caaaactaga aacacaagcg tcaaataaag ccgatggtga aaaagtggtt gatccattag
    46621 aaacgatcgt cattaaagac agtgtttctt ataccaactt gtttgtcgga aaagaataca
    46681 ccatttcagg agtattgatg aacaaggcaa cgaatgcgcc aattttacaa gatggcaaag
    46741 aaatcacaag taccttggcc tttattgcag acaaagtcaa tggcacaaaa gaccttgaat
    46801 ttgtggttaa tgctagtgtt ctgaaaggtc aaacgatcgt tgtttttgaa aaacttttcc
    46861 gtgatggcat tgaagttgca gcacatgcgg atatcaacga tgaagcccaa agtgtgaaag
    46921 tgaataatcc aaccgttcat accacggctg tctttgaaga cggattgaca gtcgcagacc
    46981 caacaggatt gtttacctta aacgataccg ttgaaattac cgagattctt ccaggaaaag
    47041 catacttggt caaagggatc ttaatggata aagcaaccgg tgaacccttg cttgtgcatg
    47101 gaaatacggt agaaacgagt gttggcttta ttgccaaagc agaaaatgaa acggtgatcg
    47161 taccatttac ctttgatttg acaggtataa atggtcgtga aatcgtcgtg tttgaaagtc
    47221 tttttagaaa tgatgaagaa ctagcaaacc ataaagacat caacgacaaa gggcaaacgg
    47281 tacgagtaac taacccaagt gtacgtacaa cggctacaca taaacccact ggcttgaaag
    47341 aggttaatcc attaacgaaa gtcaccatta cagacgctgt gaaataccac gatttggtcg
    47401 ttggaaaaac atacacagtc aacggtgttc taatggacaa agtaacggaa aaaccagtgc
    47461 tagtagatgg aaaagaagtc acaagtacct taacgtttgt ggcagaaaca acggatggta
    47521 cagtcaatct agactttatt ctagacgcac gtgcgttgcg aggcaaagaa gtcgtggtct
    47581 ttgaagatct gtttagagaa ggcgtattat tggcttctca cgcagatatt accgacaatg
    47641 accaaaccat caaaatcagt aatccaacca ttacgacgaa agcgacgaat aaaaatggcg
    47701 gaaaagagat cttggccttg ccaaatcaaa ctgtgcttga atgggtcaaa gtagacggtc
    47761 ttgcgattgg tcaaatctat cgcttaattg ttcaaggcta cttaacacca gacggcacac
    47821 catttgaagg cactcatgcg gaaaaagtct ttgcggccga taaagaaatg atggaattct
    47881 tatttgaatt cttagtggac ggtcgcaatc ttgcaggaaa aggcttgagt tttgcggaat
    47941 ggctccaagc gaaaacggaa gatgagaaag aaacgtttga agaagtggcg aaacacaatg
    48001 tagatctaac aaacaaagat caaacggttc aaattgtgga agcgccaaaa ccaccgcaac
    48061 cagtgaaacc agaacaacgg gttaaaccag ctactgttgt accaatgttg ccaaaaacag
    48121 gtgaacaaac aagtagtata cttatggttc ttggattagt agtattagtc attgctttag
    48181 gtataacact ttataaagta cgtaaagcca aagattagtc atgaggaaca agcggttagg
    48241 atttttaacc gcttgtttga ttgtaatgta gaaatattta gaatttattt tggtttttaa
    48301 gaggaggaaa aactttatgg cagaaaaagc attgcgtatg aagaaagagt ttttaggtca
    48361 agtattttta ttgggttcac gggaacctaa tccaccaaag gaaattaatg gtgtgtctgg
    48421 gcgatatgtt caattacaag cagagtgttc gcgagaagaa cagcctgctt tttttgttta
    48481 tctcacagct gaagaggata aaagattacg attaccagaa aaagaggtgc gtatttcatt
    48541 tgaagaagct tattttgttt ttcgtaaaga acgagtgaat agtcgtgaaa caaatgatat
    48601 gggacaaaca gagcgtcgaa caactggcta tcaagatgtt ggtgtctttt taactggtgt
    48661 aaaaattggg gaggttttgt aagatgggat taaaatttcc aaataattat gacattacag
    48721 aagcattaga tgaaacttgt ttaggagccg tacgctttgg cgcattagaa gagtctgatc
    48781 gtgtatttga taatgaaaca ggcgattata cgggagaaat tcgatcacag cgagcacaat
    48841 tagtttttgg aaatccagat gaaccaaaga ttgcacgacg agttaaattt ccagcagatg
    48901 tagatttgac tggttttaaa cgacgtgatc ctgtgaaatt aattgatcca gaatatcatg
    48961 tggtatatat tccagcacaa gatggcaatc gtgaatattc agatttagtg atttatgcga
    49021 aaggattaga gaaagtagaa gctccaacaa ataatcctaa tatgaaacaa tcacaagaaa
    49081 aaggtttaga tcaaaaacaa ccaagtgttg attctaagga aaaagagaaa aaataaggtt
    49141 tttttgaaag aataggaagg agtcttccta ttctttttat ctaagataga aggaagggga
    49201 gattttgaat tcagataaaa ccaatcaaat actttgagag attgtgcttg ttatcaaaat
    49261 atccaacaat tgattgagta ttttggagac aatccaacga tttttgtaca aacaaatgca
    49321 tatatttgtg cggttattgg gcagtgtatg tttttacgtg atcttgttcg tttttcatta
    49381 ccgtaccagt ggaaacaaga tcatcaggaa gtgatttgga ttcgtatagt acgaatatgt
    49441 aatatgtgtg cacaccctta taaaaatcga ttaaatcttt ttcgattatg tttttttact
    49501 aaatgtgata ttcctgtatt agttgctttt tgtcaaaagt ttttagcaca ggaaaagtaa
    49561 aaacagaaag gaggaatgtg acaattgaaa gctcattttt tcaagtatcg tggtcggcga
    49621 attcgctatt tcaatcgtta tttatggttt tgctattatg gtttcttttt gtttccattt
    49681 ttgattctgt taggttggtt tagttggaaa gtattttatc cacgtttaag tacgttttcg
    49741 aattggcaaa tgtatcttat tcctggaata agtattgtgt tgtatttgtt gctggttagt
    49801 gggttaattt tagggttgat gcattggtcg cgactaaaag aaggatattt tgcctctgtt
    49861 tattggcgtc agttacttgt tcgaatgtta attgacaatc ggtttgttta tacaaagaaa
    49921 caagttagtg agaaacaaac aagggaaaaa atgagattac caaaagttta tttttatcaa
    49981 aagaaacaag aattagtggt ttcttttcct ttagatggtg ggcgttttca cgaacgattt
    50041 ttaaaaatgg gtcatttgtt atctgaagta tttttgcgtg atttaattcg tgaagaacga
    50101 gaaaaagccc gaatcaatta tgtattcctt tcagattctg gtcggattcc tattgatcaa
    50161 tgtatagcag aaaatggacg tattcattta atgaaaactt tggattgggt atatgatcaa
    50221 tcaccaaata tgttgattgc tggtgcaatc ggtggtggga aaacctattt attgtattct
    50281 ttaattcaag cttgtttaag tgttgggaca gtagatattt gcgatggaaa agcggcagat
    50341 ttagcagcat taggtgacgt cgatgtattc aaaggacatg tgttttacaa aataaatgaa
    50401 gaaatgatta tttgtttacg aaatgcatta aaagaaatga atcaacggta tgcctatatg
    50461 aaaaccatga aagatcctaa atatgaacca gggaaaaatt atgcttatta tgggttacca
    50521 ccgcatttta tcttttttga tgaatgggca gctttttatg gttcgttgga tatggaagcg
    50581 agaaatagag tcgataaatt gattcagcca ttagtattaa agggtcgtca agcagggatg
    50641 tacattattt tagcaatgca acgaccagat gcagaatatt ttccaagtgg tgttcgggat
    50701 aatttaacat tccgtatttc tgttggtcga ttgtctccca ttggttattt aatgactttt
    50761 ggagaagatt ttaaaaataa gccattttac aacaaagatc aacctggtcg tggatatgct
    50821 tttccaggat ttgatgtacc tgtagagttt tttgcgccat ttgtttacaa agattatcac
    50881 tttatcaaag aatttgagaa atttactgaa atgattgtct atgattttac agtaaaagag
    50941 gatagtcaag atcaaacgat gggagatgat aatcagttgt tacaagaaca actagagcgc
    51001 caaatgtatt tagcaaaaga tccaaaagga acaagttttt ctgatgcgca tattttgtaa
    51061 tcaactaagg aggtgaagtt aaatggcaga gagttgttat tatagagtag ctacagcaat
    51121 acgaatgatt aatccttctc tttcttccag aacgttttat gattggttga atcgtattga
    51181 acaagtaact gattaccgct ttttgcgcaa agaacgtgtt tttacaggaa aagttattaa
    51241 tcaagtattg ctaacgaaaa aagatataga acgattgact cgattgtatc attatcgagt
    51301 agatttagaa gaggatttaa ctttgtcaat ttatcgcgtt ttttctcctg aaaaatatag
    51361 tgaaataaca aaattagatc atttaatttt gtagttatta tctatcggga gggctctaga
    51421 cgtagtctgg tagccgcact ctgcctggcg gtgcgcatta aggctgcgaa gcatgggcct
    51481 ataatgcgca ctcgctagca gcgtgtgcag ctcacaaaga ggtcgtgcgc ttgcgcacgg
    51541 cctctttgtg taaagccccc cgatatataa tatggggggc agaaataaca taacacatag
    51601 aaaaaagttt tataaaccct tttgtatcaa gtgattatct aaggtcagca gttgtactac
    51661 ttttcccaaa aaacgagggg gactagttga tatatcggtg tttctaaagg gttaaaaaaa
    51721 atcgattttg ttattttata aaaggagcga gaaaatgaca ccgattgagc taaaaagcta
    51781 cagaaagaag ttaagtgcaa ttcataatat aagtggagat aaaaagccgc tatcacaagc
    51841 aagatttgcg caattgatgg gctgctcaaa agattacatt agaaagatgg aacaagggaa
    51901 aagaaaaatc ccagaggatt ttgaacaacg aatcaaggaa cgctttcgat tgtatatgaa
    51961 aagcggaata gatcttcgag caatgattga ttatttgcgt ctttcctttt ttgggacaga
    52021 ctacaaaaca gtgattcaac gtgtattacg aatggatctg acagaatttg aaacacgtcc
    52081 aacgggcttg tatctttttg actgtctttc tgttcgtggg aatatctggg tgttctggca
    52141 ttcaaaacga actgtaacag aaaatgtact tgtgcaattt tcaggacaag gttgccggga
    52201 atatgaggaa gtcatgcgtg aacaacaaac agattggcga acatggctat attccatttg
    52261 gaaaaatcaa ggaaagttgg aagctggtta tacacgtgtg caatgtagcc gattcgatat
    52321 tgcgttagat gaactttggg gattggaaac ggaagaacat ttcgatctct ttgaattact
    52381 agaaaaacaa aaagcaggtt tattagaaat ggactttaag agattccaga atattggcgg
    52441 ttgtttttta gatgatggct attttcgaag tgaaggatta tctttgtatt ttggatcgcg
    52501 aaagagtcct ctctttttta atttttatga gaaacgtttt gaaattgcga accgagaaaa
    52561 aatttcagaa ttagaagcat taacgaaata tggtatttat aatcgatatg agttacggtt
    52621 agccaatgag aaagcaacac aagcagtaga agcatttatt ttaggtgatt caccgaaacg
    52681 tttaggagag attggcgttg gtttaattaa tgcaaaactt attgtttggg atagactgga
    52741 aagtgatgca cggattgttg ataaacgatg ggcaaaatta tttcgttcga tggaagaatt
    52801 ggattttaca gtaaagccag aaaaattctc attagatcgt tcagaacgct ggtttttgaa
    52861 acaagtagct ccgacattaa agttgttaca agaagcagat cgtattttag gaagagactt
    52921 tgtacaagaa gggattgata atgccgaact aaatgacgag caacaaaact atctagaatg
    52981 gatgggaacg tcaggaggga aaattgcatg aatgcatata cgacaacaga atggctgcgc
    53041 ctgttggatt taaaggctgc aatcgaagca ttaaatgaaa agatggttga tttgtcttat
    53101 tttcgttttc gagttcctta tattgaacaa gcggtaaaag ctggacgtta tcaggaaaaa
    53161 gagaactggc aagaaattgc acgcttatta gaagttcgta agggatacga acaagaactc
    53221 gaagagttgg aatttagccg aaggaaagga aggctagaat tcattcgctt ttatcgattt
    53281 tcattgccaa tacctgcgat tttagcggtt aaaaaaggct gcgataaaat gaagatatat
    53341 gaaaactgtg tggcagcact tagcagcgaa aaacctctta tggaagaaat ttcattggtg
    53401 actgtcactt gggaaatgag tcgccagcca acagaagaac agacatatct ttctttagaa
    53461 gagattgaga tagaattaga ggaaattggt cgctatgcca cttgttctac ctattgtgga
    53521 tcggtcatta gtattgctgg tgtgattgtc taaatgaaaa aaattatgat ttatgttggt
    53581 gcgtattggt cacgtgatcc aacagtatta gaaaatccag aggcggtaca ctattgcttg
    53641 cgccaattat tctatctgta taaagaacga ttagaatcgt tgattcgtca gttaccatat
    53701 actgacagac gtttagatga acttcttcta aggtatccag caatgtataa acgacgaaaa
    53761 aatagattgt taccagaaga gtatcccatt gaaaaacatg aacttgaagg gcgatttgtg
    53821 gcttatttct atgatgatgt tcgtatgcgc ttagtagaac aacgaatgga aatagaaaat
    53881 gatcgctact attttatcaa ttattgtaag aagcgtaagt atcaagtaac tgatgatttt
    53941 tatgattgta ttttacaaga tggggaagtg attctatcca aaattgctcc aagttttcgt
    54001 gaacttgtgc cgattgattt tttgaaaaaa tgtcatttgc ggattcttcc gtaaaaaaga
    54061 gctataaaag gaaagaaaag tagaagccaa atcggtttct gcttttttat tttttttagg
    54121 aaggagaaga tggtatgtca ctggaatttt taggagagcc aattgaaaag gaagagttcg
    54181 tttcgcatta tatggatttg tttattcagg tgataaaaaa ctttgtgcga ttgatgaatt
    54241 ttatccacgc gaacaagact atctagccgc tgaacgaata aaaatcagtt tattatggga
    54301 aaaattcaca gatgaaaata accgaccgcc agattatcgc tatttatcag attgtttaac
    54361 gaataatgtg ctttctaaag aatacctatt tcacaaacgt gagaaaaatc aaatgtcggt
    54421 tgaacatttt tgtgattgtt atattgcatt attgcgtagt caacagttat taacagtaga
    54481 acgatatggt gcagagtttg gttatgtcat gcaagctgag cgtcatagag ctacaagatg
    54541 ctatgaaaaa gaagatcaag tgattgatgg ctatgaagaa ttatgtattc gaaacaatcg
    54601 ctatttgcaa caacgtttat atgaacaatt gatgatggat ttttcaaatc tctatagagg
    54661 gtatgaatgt tcgatgattc aagaaaggta gaagaaaagg gggaattttt atgtggcgat
    54721 tattattttt ggtggtatgt ggtatggcat tgttctatgt gtatcgtgtc tatctctatc
    54781 cacgaatgga acgatggaca agttggtata gagatccttt aaaacaacga aaaggattga
    54841 agcggtatcg ttatgaatta cttgttgaat tttcggatgg gcgacaagca cagtattatt
    54901 tttttgcaaa ctatccaaaa tacaacacca gtgaactatt gattcaatta ttcaaaaata
    54961 atccatatgt tgggttagaa gaaaacggac gggagttctt ttatcgttca gataatatta
    55021 gtgcggcgtc tatgaacaaa acacgaattg cataaaaaat tggaggaata atcatgaaaa
    55081 aaacaacatt agcagtcgta acaacactag gaatttcatt attttcgttt caattaggga
    55141 gttcattggt ttatgcagat gaaactgtag aaacacctaa gtcagaaatt gtagaaacat
    55201 tgccagggga aggattacca gatgttgaga cagagctaaa tggaggaaca acggaaaaac
    55261 cagcagaacc tgaacaattg cagtcagaga aacctgaaaa agagccagtg acaccagaac
    55321 cagagtttcc tgaatcaact ttaccgactg attcgatcga agaagaaggt gtgcatagtt
    55381 caacaacaac ggattcaagt atagtcacaa ctccggaaca atcaggaaca accagttctt
    55441 ctgaaaagaa tactgattcg tcaagtacgg aacagccaac gatgtcgtct accagtgaag
    55501 aacaaaaaga agaaccgaaa caacctgttg aaaaatcaga agcaattcca aaagaaaata
    55561 aacctattgt tccagcaaaa gaagtcacag tatcggtaac cccaagcggt gaaattacga
    55621 cgaatacttc tcaaggaatg agtgtaccga ttgtcactag taatgtagag gaattgaccc
    55681 atattccaac accaaccacc ccattaaaag tagcaggtga tcaaacaatt gttggtgtaa
    55741 aagatggtat tccattaatc caagatagtg aagggaattt ggtaaaggat ttgtcgattc
    55801 ctgtaaagaa acttccaagt ggcaatattg aaatcaaaac agcagatgga aaaacaaaag
    55861 tcttaccaaa gactggtgaa gaaatacatg ttgcactttc tgttctagga ggagtgttga
    55921 caagtattgc tggatttgtt ggatataaaa gaaagagaaa aggaatagaa aatgagggta
    55981 attgaatgga ctatttttca gaaaaacaag cttatcgttg tcaatcgtac gtactagtaa
    56041 aaacatacta gaaggagtag cttatgaaaa aacgacaact acggcggaaa gaaaaagttt
    56101 atacagagaa agtaccgaag caaaagaaaa tccattcaaa aaaagcagtg aaatggattt
    56161 ggtgtttggt tttatttctt tgtttttcag ggggcttggc attttttcaa tccatgaaaa
    56221 cgaaggttca agtgtcgttt ttgaaagatg aaatccaaca attacaaaag aaagaagtac
    56281 aaagtcggtc agaattaacg tacacacctg aaattgaatc ctttatgaat cgttttgttg
    56341 ctttgtatat gaatacattc gataatcatg aacaagaaca acaacgtcaa gaaacattaa
    56401 aaaaagagtt ttatgcacaa aactttattc aagaaccagg aagtgtttca ggtgttcgaa
    56461 aattaactgc tgcttcgttt attggattaa aagaaattga aaccattcca actgctagtt
    56521 ataaagtaac ttatgatatt cgctcgatga atccagagag taagaaagaa acaagtgtga
    56581 caatcacaca agtactcaat cttccctttt ctatttcaaa caagggctgt cgtattattg
    56641 cttatcctta ttttacggca ctaccaaaga atacagaaaa acaacgaatg attacttatg
    56701 atgacacatt atacgaaacg gttgatgcgt cggaagcgaa agatgttgaa ggatttgtgg
    56761 aagatttctt agataagtat gcgaaaaaca atgcaaaaga tatgtcatac atgatgaaag
    56821 aacctgaagg actcaatcaa acagctaaag ttgatcaagt agagatgaaa gtattgcgtt
    56881 ataaagaaac gaaaaaacct tttattgtaa aagcaacggt cgtctttcga gatggtgata
    56941 cgtcactttt acaaaaagaa caaatgacga ttgtcattac gaaaaaaaca aatcagtatt
    57001 atgttgaaaa attaacacat aattggttgg ataggaaagg agaagaacgg aaatgacact
    57061 gaagggaatt gtagatttta tattaacgca aggtggctat tttttaataa tggtggttgt
    57121 ttatctggga ttgcgaaatt ttatgaaatc aaaagtagga aatgcattaa tatctatcgc
    57181 agcaggtgcc gcagtttatt tcttcttaaa tgatccaaca cgtgtatttg atgcgtttgg
    57241 taaaatcatt gaaaaattat ttatggatta ggaggatagt gaagtggatt gggaacctta
    57301 tgattattcg attgtttatg cggaacctta tcgcgtacaa cagattacaa gcaattggcg
    57361 tcttccattt ccagttgcat tagtcaatgc agcaactttt ttgttggtcg taatctttga
    57421 atatttattt ttacgttcat ttattggttg ggtcatttct ttttttccac cagcaggagt
    57481 tgttttttat ataggactcc cctatttatg tgtgttagca ctggcaaaac taaaacctga
    57541 tggaaaaaaa gtgcattttt atttatggga ttggttttgt tatatgattc gtatgatgaa
    57601 tggtgattgt gtgtattata actgggacaa ggtatcaaac aatgcattgg aagaattact
    57661 ttttgtgaat aagtatgttt atttagagga ttagaaaaag ggacaatcaa ctggttgttc
    57721 cttttttatt gggaggtgaa attcattggt gcttgtaaaa gaaccagtaa aagcattcaa
    57781 gaaaaattgt ttgttaaatg gagaggattg tatctgggcc tactatcatg tttttccacg
    57841 aacaatttca aaaaacacac caaaaaagac acaggaaata aaagaaatga tggctcgttt
    57901 actgttgctt ttagcagatt atgaagatgt ggagttggtg atgtatccac gaacaatgaa
    57961 tttacggacg aagtttgaca cattaggaaa gggattttct cccgatagtc aagcaattgg
    58021 tgcgtattat gcaaaagaaa cagtgacatt gttggaacga gaaggtcaag tctatcgtcc
    58081 ttcatttatt ttaggaatca aagtcaaaga aaaagcaacg gtggagagtt ttcatgatgt
    58141 aataaatcta atctggttag atttagcagg aagtttagca cgcttatttg gattagaagt
    58201 agatgatttg gaaactttgt tttctgaaag tgatccttat gtaaaagaag tgacacaatt
    58261 actttatagc ttttctattc gtcccttagt aaaaaaagag ctgttgtatc tcaatcgtct
    58321 gcaatatatt cgaggaatgg ctcacaaagt agaagaacaa aataattggg aagtactgaa
    58381 agacaatcaa ggaacgtatg tacgtatacc gaaggaacat aaaggccttc ttcaattaga
    58441 agatgagtta gggagtagtt atttagcatt gttgcctatt ggggaaacac ctgaaaatat
    58501 gagaaattgt gatctatttc aatttgctca ggatctttct ttcccagcag aattgcgaat
    58561 gaaaattcac tatccacgct tgaaaggaca gggaggtatt ggtttaaaac tgaattggct
    58621 atcggaacgg ttcaaaacag agcataagga attggttcaa aatgatgaag aacctagtga
    58681 acgcttatta actgttcgag gattagtaaa acatttaaag caacgagtag ataatgggca
    58741 acctgtcgtc gattggatcg catgttttat tgtatacggt caaacggttg aagaagttcg
    58801 tcaaaatagt gcaacggtta ttcaacaact tcatacgatt atccatatcc accgtgcaaa
    58861 aaatgatcaa cgagaattgt tctataaatg tttattaggg caaccattgg gacggcagtt
    58921 acattggaag caccgaacaa ctgtagaagc agttggcgaa tggttgtttt cagcagtatc
    58981 agaattaggt atggaatccg gctggtatat tggtcggcaa gatacattag gggatggtgg
    59041 ttctcgagga gaaaaaacac cattagaaga attaatttat gcgtcaaatc gttttgtctt
    59101 tttgaatctt ctagccattg cggaaggaat caaaggtgcg ttgtttgatg cgcctcatgt
    59161 agcagtaact ggaaaaaccg gtaaagggaa gaccttttta gttggcttgc tctttttgta
    59221 tagttcgttt atgaatgtac aaagtttatt tgtggatccc aaaggagaaa agcgcaaatg
    59281 gtttacgaaa ctcattcaag acccgtatta tcagaaacat taccctttgt ttgtagaaca
    59341 tttgcaaaaa atgagttatg tcacgttaga tgctacaaat ccccgtaatt atggtgtatt
    59401 agatccaatt gtttgtttat cccgagtaga tgcgaaacaa gtagcccagg atatgattga
    59461 tgagttaagt ccgttaggta ccaatcacct tttaaaaggg gcggtattaa agggaatcca
    59521 agaagtttta gttcaaaaag aagccggaca acaagttggc ttgatgcatg tggtcgacta
    59581 tttagaacaa atggaacaaa aagaggttcg agaatatggt gagtttttac gcttgacggt
    59641 agaagattcg attttacgtt taggattttc ttatggagaa aacccgggat tagactttaa
    59701 tgccaaaaca acgattttag agatccaaga tttgaaatta ccaaatgaca atgtccgacc
    59761 agaactatat acagatgcgg atcgaaagtc tttgtgtttg atgattagtt taggtcgttt
    59821 ttgtgaaatg tttggaaaac gagatagtag taagaaaaca gcgatttatt ttacagaagc
    59881 atgggtattt aataattcga acgcaggacg tagtattatt gcagcaatgg cacgtgtggg
    59941 gcgttcccag atgaatcaat tagtgttaga tacacaattt attggtgact taggttcaga
    60001 taaagaaaaa gggaattttg gtgtcgtctt tgcttttgat gaagacagtg agcgagaaaa
    60061 gattttgaat catttacgtt tggaagtaaa tgaagcgaac ttagctgaaa tgagccacat
    60121 gattaaagga cagtgttgga tgttagatcc ctatggacgt gtaggaaaac tcaatgtaca
    60181 cagtttgttt gaagaaatga ccgctgcatt acaaacaacg gaaaaaacaa gtcactctca
    60241 agtagaagaa caggatacgt taatcatgca atagaaatga ggtgaaacaa tggcttatat
    60301 aaagcataag gaaaagaaat ggtgcgtttt gtttttttta acgattggat tcggactgct
    60361 ttttgttggt ggattggtgt atgcggcaga aaagcaggtg gatcaatcga tctatgagat
    60421 tgggaaattt catattgata ccttttcttc ttatttatct gatagtgaat tttcattagg
    60481 caaaatgtta tttggggcaa atggcacaga ttttctaaaa atgatgacga atatgttctt
    60541 tagtttaagt aaattgattt ggcaaggcct tgattttgtc attgagaagc tctatgaagg
    60601 agctgcaatg aacgagttaa ttcaccagtt tttcactttt tcggatacta tttattcaaa
    60661 aatatacgat cctattggtg cggggattgt cgtcttgtat gtgttatatc tatttactct
    60721 tcgtatattt agagggaaac attacgcaaa aatggtgttt attcgatttc tcttcgttgt
    60781 cattttttca atggtttggt ttggctatgg tagtaacgta cctagtaaag gagaagcttt
    60841 taccaaaggg ttaaatgatt tgtccgtgga ggtagaaggc tttatttttt ctgctacaaa
    60901 tagtctagat gaattggcga caaaagatgt caatggtgtt ttagtgccaa cgacacaaaa
    60961 ggaagccatt caaaaagtgc gccaacttta ttataaagcg gctgttgttg atccgtatat
    61021 attgttaaat tatgggacaa caaatttaga agaattgtat caagcagaga ttgaaccaca
    61081 agaatttcta gccaaggaag cgtcaagtga agaattatct gatattgaga caaaagtgac
    61141 cactgctgca ggagaagata gagggaaaaa gaaggcttat cgtgtctata ttcaaccaac
    61201 taaagctgtt tataaagcag tcgttggttc tatggtgcca ttgcttaatc taagtattgg
    61261 attaccactt gtagtgattg gcatgattcg ttttatcttt caaatgttgg tgttgtgttt
    61321 attacttgtg attccttttt cattactcgc ttcttttgtt ccagtatttg attatatgct
    61381 catgaatacc ttaaagtcct ttttgggctt gatgtttcaa aaatcttttt acagtgtgat
    61441 tattttggtg gcatttttga tctataacat agtagacgat ttaattccaa tgaatactgt
    61501 ttttggtttt ttaggaaata tgtttgtcaa agcactccta tcaattttac tcattgtaaa
    61561 acggaaagat ttattaaaga aactagggtt aggagctgca gatcaaacta taaacaacac
    61621 gaaaaacata gcaagtcatt ctttgtctca aatgaaacaa ctgcctggtc aactatcaac
    61681 tgctggacaa aatttggcaa tcaaaggtgc aggattagca gggaatgtgt atcgagcaac
    61741 gaatccactc gcttctgcag cgatacatgg atttcaaaag tttcaacaaa aacgtacacc
    61801 acaagaatcg gtaaaaggga ggcaagtggt taatcccttt gaactaggtg ggcgttcaaa
    61861 tcaagccttg ccttatagtg gtcgtcatgt tcgttatcat ggggtagagc ctgaaatgtc
    61921 ttctaccaaa tcgaaaacag agtggaattc tggtctttca aatggtttga ttccaaattc
    61981 gtccatgaat tcagtaagac caaaacaaca ggtacaatca ttggcacaat tgagtaagca
    62041 acaagggaat aatcaaagaa aaccattgtt aaataaaggt atcgcttttg ttggtcaatc
    62101 gattccgttg ccttctggaa aagtacccgt gccacaatta acagctgctg gttctctttc
    62161 acaacagatt cgccaagtga aaaatcaggc gaaaaatggg agttatccag caatgcagca
    62221 gttattgaac caatactctg ttcctaattc gtcggtagcc actctatcag gaaatcgttc
    62281 tgtgcaacaa ccggggcaac gttcgtctga aagtaaaccg tcgatttccg caccaaaatc
    62341 gttacaacct gcattttctt ctttagataa gccaacagag caatcggaaa aaatagaaaa
    62401 ggatcctaaa acactgccga ttttggacgc aaaaccagga actgatttta ctttaccaaa
    62461 aggacgaaca gtccaaaaac cattatcgaa gaaaggatga ggaagaaatg aagaaagggt
    62521 gtctaggttg tcttcttttc tttattcttc ccttttttct attgccattc tttttttcag
    62581 gagaactttc tgagcatgga ggagaagcta caggtctagc tgtcgctttt aatggggaat
    62641 ataccgaagg attaccgatt ttttcagaga ttaaaggacg tggtccaatc acagatgaac
    62701 atgcgcaagc agcagttggt gcagctgtaa agtatcagtt attaccgagt gtgattcttt
    62761 ctcaattagg ctatgaatca gcttatggac agtcacaatc tggaagaaac gatacgaact
    62821 tttttgggat tacctggttt cctggttgtc cctacccaca aggttctgca cgtggaattg
    62881 gtggttctga aggtggtttc tatatgaagt ttccgaactt aaaagcttgt tatgactatt
    62941 atggctttat ggtggcgagc caaagcaact ttaatcaatg tgtgggcaat aaagatcctg
    63001 gaagttgttt attgattcta ggtcgtggag gctatgcggc agcaggtatt accgaaggga
    63061 gtccgtatta tcgaggagca atgagtatca taacgtccta taacttaacg gaatatgata
    63121 cttttgcgat caagcattgg aatagcctac caacaaaaaa aacttcgctt attgcaggaa
    63181 aaagtaaagg caatgcgaag gttttggatc ggatcattgg acgatccgta aataatgggg
    63241 aatgttatgg tggtacggca tggtatgtat ctcaattagg tggcccacag ttaatgggtt
    63301 ctggacacat gtttgcgatg ttaatagggg aagattataa ctggacaacc tatggttggc
    63361 aagtaatcat gaacccaaaa ccaagtgatt tacaagcagg agatgtggtg aattggcaag
    63421 caggtggtgc attgtctcct ggaatctatg gtcacaccgg aattataaca gatgtttcat
    63481 atggcggaca acatttctct agtgtggaac agaatgccga gcaaggacgg attatggctc
    63541 ggtatcaacg cacgtacaat caaacaaaaa ttcgtagtct ggttcgtaag gtgaaataag
    63601 aaaggcagtg actagaaacg tgaaaaaagg gataatttta gtatttagtt ggttatgttt
    63661 gataggtctt actggttgtc attggttttc ttctgaaaaa caaaaaacac aattgagaac
    63721 gacagaagaa acaatcgaag cagtaaaaga aacaagtgga actcttcaaa gtactgaaag
    63781 taacagtcaa attggttcat tagaagacga gacagcgcaa caaatgcaaa tagatgaaat
    63841 gacaaaaaat ttcttaaaag attttgctag aaaatggagt aatttcacag atgtttataa
    63901 acgcaatcaa gctgtgaggg actatatgac agatcgttgt attaaagaca atagtattga
    63961 tgtagacccg catgtacaaa tgaaaacaac tgggaaactt taccaggtga cacaagatat
    64021 agagaatccg gatcaatatt tgttatttgg agaagaaaca atgaataagg ttactcaatt
    64081 tgtattactt caattgaaag tagatcaaga agctcaaaag attgatgcga tcaagattta
    64141 ttatgtacgg agtgcgtatt aaaataaaaa atgaacactc atatttatta tacaccgaag
    64201 aaatagaaaa aaacagactg tttttttaag aaaaactatg tcattcttta cttgaaaaaa
    64261 gttgttatag gacagaaagg ataaaacaaa aacggaaaaa atatgtctta tgaataacta
    64321 taagaactat tctatttttt aggagatgga tacgagatga aaaaactaat tggctatagt
    64381 gtgatgtgta ttggatttgt tggtgtggga ttgtttgtat cgactgaatc tgcttctgcg
    64441 gctgaatggt ataatcctat ctggtataat ccgaacattg aatgtcaaaa aggctatccg
    64501 tatggaccga caaatctgaa aaatggaaaa tgtagagtaa attggaatgg cgttgtagat
    64561 agtgtaatta ctggttcaat ggactctgct ctacatggct ttacgggtca ctagaaggag
    64621 gaagaaaatg tctgaagaga aaaaagaaat cgttgaatca ttagtagctt taaaagattc
    64681 tctttctaag attgaatatg ttgataggca agaaattcaa aaactaattg atgatactat
    64741 tattgaaatc caagatgcgc gttgtgaagg tataaaaatt tctgtagctt tatctaaagt
    64801 aatagaaaaa atgaaccgtt cattagcgtt taatggattg aagctagata gacaaactag
    64861 tctgatatgg gatcatttaa aagacttgta tgacaagtct aaaagaagtg aacggactgc
    64921 tgtatcaatc ttaaaaggcc tttggggaat gaattcataa acaagatatt taaaaagtaa
    64981 cttatttttt atggaggaga aattatgaaa agaataatta aaatatttgg tgttttaggt
    65041 gttttcttat taggtttaag tacttttgtt agtactggaa caagtgtgta tgccacagaa
    65101 aaagtgaata cagttgattt agaaataaag aataaattag tagatgaatt aactttttat
    65161 tttgatacag ttggtttttt agacgaacat ggtgtatacc atataaagga ttttggtgct
    65221 attcgtgaac gaatcagtca agataacgaa caaggaagag cagcaagagt cttttatgaa
    65281 catattattg ttcctagaat ggagaaagtt gattggaaac gatatgctgg atgtgtagcc
    65341 attaattctc ttcctggagg aggagtggct tgggctatag gtacgcaggg agattttatt
    65401 gaagcattaa aagcttttaa ttttgaaaaa gctgcggatt tattattaaa aattgcagaa
    65461 aaacaattag gaaaagctgc agctaaagag ttagctaagg taaatattgt agcaggaatt
    65521 gcagcagctg ctgctagctg tgcattagag taaaagttta tgaaaagttt agaacaagag
    65581 catatggctt ttaaacaagc aatgtttaaa gaaaatatct atttaaacca taactatatt
    65641 cgtgtttcta aggcatgttc accagtattg aatatgcttg gaggtggaaa tggattatat
    65701 catttactgt ttgtcgatgt ttgttggtta atttttttac cagatgaact agttatagtc
    65761 aatgaaaaaa taacaagtaa aaatgaagtg tttaattatt ctttaacaag aattaattac
    65821 aaagagatta ccaagttttc actagaaaaa gttccttttt ggggagaatt ttgtttaaa
//
LOCUS       DACDUU010000007        57517 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.007, whole genome shotgun sequence.
ACCESSION   DACDUU010000007 DACDUU010000000
VERSION     DACDUU010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.007"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..589
                     /locus_tag="IWL30_000473"
     CDS             <1..589
                     /locus_tag="IWL30_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705266.1"
                     /translation="FLDMNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYF
                     GNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLI
                     VGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMA
                     VKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
     gene            complement(829..912)
                     /locus_tag="IWL30_000474"
     tRNA            complement(829..912)
                     /locus_tag="IWL30_000474"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(876..878),aa:Leu,seq:gag)
     gene            1295..2512
                     /locus_tag="IWL30_000475"
     CDS             1295..2512
                     /locus_tag="IWL30_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9705267.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            2696..3532
                     /locus_tag="IWL30_000476"
     CDS             2696..3532
                     /locus_tag="IWL30_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9705268.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            3608..4756
                     /gene="nagA"
                     /locus_tag="IWL30_000477"
     CDS             3608..4756
                     /gene="nagA"
                     /locus_tag="IWL30_000477"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP9705269.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            4977..6035
                     /locus_tag="IWL30_000478"
     CDS             4977..6035
                     /locus_tag="IWL30_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAP9705270.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            6054..7073
                     /locus_tag="IWL30_000479"
     CDS             6054..7073
                     /locus_tag="IWL30_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAP9705271.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(7175..7891)
                     /locus_tag="IWL30_000480"
     CDS             complement(7175..7891)
                     /locus_tag="IWL30_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP9705272.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(7860..8645)
                     /locus_tag="IWL30_000481"
     CDS             complement(7860..8645)
                     /locus_tag="IWL30_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9705273.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(8636..9385)
                     /locus_tag="IWL30_000482"
     CDS             complement(8636..9385)
                     /locus_tag="IWL30_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAP9705274.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            9683..10207
                     /locus_tag="IWL30_000483"
     CDS             9683..10207
                     /locus_tag="IWL30_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705275.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            10223..11722
                     /locus_tag="IWL30_000484"
     CDS             10223..11722
                     /locus_tag="IWL30_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAP9705276.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(11810..12412)
                     /locus_tag="IWL30_000485"
     CDS             complement(11810..12412)
                     /locus_tag="IWL30_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9705277.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            12516..13472
                     /locus_tag="IWL30_000486"
     CDS             12516..13472
                     /locus_tag="IWL30_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAP9705278.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(13777..14733)
                     /locus_tag="IWL30_000487"
     CDS             complement(13777..14733)
                     /locus_tag="IWL30_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9705279.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            14907..15467
                     /locus_tag="IWL30_000488"
     CDS             14907..15467
                     /locus_tag="IWL30_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705280.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            15460..16285
                     /locus_tag="IWL30_000489"
                     /pseudo
     CDS             15460..16285
                     /locus_tag="IWL30_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
     gene            16295..16912
                     /locus_tag="IWL30_000490"
     CDS             16295..16912
                     /locus_tag="IWL30_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAP9705281.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            16930..17613
                     /locus_tag="IWL30_000491"
     CDS             16930..17613
                     /locus_tag="IWL30_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP9705282.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            18296..19579
                     /locus_tag="IWL30_000492"
     CDS             18296..19579
                     /locus_tag="IWL30_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9705283.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            19595..20482
                     /locus_tag="IWL30_000493"
     CDS             19595..20482
                     /locus_tag="IWL30_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9705284.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            20499..21335
                     /locus_tag="IWL30_000494"
     CDS             20499..21335
                     /locus_tag="IWL30_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9705285.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            21338..21961
                     /locus_tag="IWL30_000495"
     CDS             21338..21961
                     /locus_tag="IWL30_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705286.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            21958..23250
                     /locus_tag="IWL30_000496"
     CDS             21958..23250
                     /locus_tag="IWL30_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9705287.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      23609..23706
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            23822..24403
                     /locus_tag="IWL30_000497"
     CDS             23822..24403
                     /locus_tag="IWL30_000497"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP9705288.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            24406..25713
                     /locus_tag="IWL30_000498"
     CDS             24406..25713
                     /locus_tag="IWL30_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAP9705289.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            26129..26617
                     /gene="purE"
                     /locus_tag="IWL30_000499"
     CDS             26129..26617
                     /gene="purE"
                     /locus_tag="IWL30_000499"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAP9705290.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            26601..27734
                     /gene="purK"
                     /locus_tag="IWL30_000500"
     CDS             26601..27734
                     /gene="purK"
                     /locus_tag="IWL30_000500"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAP9705291.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            27747..29042
                     /gene="purB"
                     /locus_tag="IWL30_000501"
     CDS             27747..29042
                     /gene="purB"
                     /locus_tag="IWL30_000501"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAP9705292.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(29222..29599)
                     /locus_tag="IWL30_000502"
     CDS             complement(29222..29599)
                     /locus_tag="IWL30_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705293.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            29855..30583
                     /locus_tag="IWL30_000503"
     CDS             29855..30583
                     /locus_tag="IWL30_000503"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAP9705294.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            30583..30837
                     /gene="purS"
                     /locus_tag="IWL30_000504"
     CDS             30583..30837
                     /gene="purS"
                     /locus_tag="IWL30_000504"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAP9705295.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            30839..31510
                     /gene="purQ"
                     /locus_tag="IWL30_000505"
     CDS             30839..31510
                     /gene="purQ"
                     /locus_tag="IWL30_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAP9705296.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            31511..33733
                     /gene="purL"
                     /locus_tag="IWL30_000506"
     CDS             31511..33733
                     /gene="purL"
                     /locus_tag="IWL30_000506"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAP9705297.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            33718..35157
                     /locus_tag="IWL30_000507"
     CDS             33718..35157
                     /locus_tag="IWL30_000507"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAP9705298.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            35189..36232
                     /locus_tag="IWL30_000508"
     CDS             35189..36232
                     /locus_tag="IWL30_000508"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAP9705299.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            36229..36807
                     /gene="purN"
                     /locus_tag="IWL30_000509"
     CDS             36229..36807
                     /gene="purN"
                     /locus_tag="IWL30_000509"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAP9705300.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            36804..38339
                     /gene="purH"
                     /locus_tag="IWL30_000510"
     CDS             36804..38339
                     /gene="purH"
                     /locus_tag="IWL30_000510"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAP9705301.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            38355..39629
                     /gene="purD"
                     /locus_tag="IWL30_000511"
     CDS             38355..39629
                     /gene="purD"
                     /locus_tag="IWL30_000511"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAP9705302.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            39751..40665
                     /locus_tag="IWL30_000512"
     CDS             39751..40665
                     /locus_tag="IWL30_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAP9705303.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(40798..42363)
                     /locus_tag="IWL30_000513"
     CDS             complement(40798..42363)
                     /locus_tag="IWL30_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP9705304.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(42356..43807)
                     /locus_tag="IWL30_000514"
     CDS             complement(42356..43807)
                     /locus_tag="IWL30_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9705305.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIRESKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(43821..44837)
                     /locus_tag="IWL30_000515"
     CDS             complement(43821..44837)
                     /locus_tag="IWL30_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9705306.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            45057..46145
                     /locus_tag="IWL30_000516"
     CDS             45057..46145
                     /locus_tag="IWL30_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP9705307.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            46199..47725
                     /locus_tag="IWL30_000517"
     CDS             46199..47725
                     /locus_tag="IWL30_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP9705308.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            47727..48932
                     /locus_tag="IWL30_000518"
     CDS             47727..48932
                     /locus_tag="IWL30_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9705309.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            49189..50166
                     /gene="guaC"
                     /locus_tag="IWL30_000519"
     CDS             49189..50166
                     /gene="guaC"
                     /locus_tag="IWL30_000519"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAP9705310.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            50430..51854
                     /locus_tag="IWL30_000520"
     CDS             50430..51854
                     /locus_tag="IWL30_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP9705311.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            52315..53424
                     /gene="pdhA"
                     /locus_tag="IWL30_000521"
     CDS             52315..53424
                     /gene="pdhA"
                     /locus_tag="IWL30_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAP9705312.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            53427..54404
                     /locus_tag="IWL30_000522"
     CDS             53427..54404
                     /locus_tag="IWL30_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAP9705313.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            54429..56072
                     /locus_tag="IWL30_000523"
     CDS             54429..56072
                     /locus_tag="IWL30_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAP9705314.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            56080..57486
                     /gene="lpdA"
                     /locus_tag="IWL30_000524"
     CDS             56080..57486
                     /gene="lpdA"
                     /locus_tag="IWL30_000524"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAP9705315.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
BASE COUNT        18433 a         9891 c        12660 g        16533 t
ORIGIN      
        1 ttttttagac atgaatatct tatttttatt gctatttttc ttaattttcc ttctaacata
       61 taggaaatgt caaaactatt atgtgcgtat attgactgca ttaccgtttt tcttaaacct
      121 gattatttat tttggtaata caatgggtca aagctttact tacgtcaatg gaaataaaag
      181 ggcaatgatt tggagtagta gtaacctgaa taatctcttt acagagttag gaacgaaact
      241 gtcacttttt tatccaggta cttggatagc tactttagta gtcttggcat tattactatg
      301 tttaattgtt ggaatttatt taagttttga caataagaaa acttctattt ttttagttat
      361 tttgatgata atgggatttt gttcaaggtt aattatggga ttttctccca cagtatgggc
      421 ttccggaatg cggacgtact atatacttta tgttgttatc gccatcttag tactaatggc
      481 agttaaagag ctaatgaaaa gcatgagtgt acaaaaaaat gaatttatgc aatttggatt
      541 aaccgtgctt ggtatttgta cctttataat aacggtgata aacagataat atacaaaaaa
      601 tccctcgctt tagtattagg tgagggattt tactcgtttt tcgattaatt gttttaacac
      661 ttgcaagttt ctacgagtag cttgtttccg accttcatat actcacctca ggctgtttac
      721 atgattgtac gttctttttg taaaggatac agtgatatgt atataatggc ataaagaaac
      781 gttcaaacaa aaaaactcgt gaagcaaaat taacttcacg agttgtagtg cagatgatgg
      841 gactcgaacc cacacgagct aacgctcaca tgaccctcaa tcatgcttgt ctgccaattc
      901 caacacatct gcgtaataat aacacactaa tgatagcgag gttttacata gttgtcaagc
      961 tcaaaaatgg gaggacttat taagatggag aaatatgacg gagaattttc aggactggga
     1021 atgatcttag gtgtattgat tggacttgca ttcggacgtt ttttgcttgg ttttatgcta
     1081 ggaatcattt gtggaattgc aatggattgg gcagcgaatc tatggaatga ctatcatgac
     1141 aattaagttc tcaacgcttg tttgtgtttt tttaatactt acgtttttcc taaataagtg
     1201 gcaaaatttt ctaggatgga gtatgatatt gaaatagtaa ataacaaaaa atttggaaag
     1261 ttctaacttt ttgaaatgag ggattggcta agtgatgaga gaatttgaaa aatccaacaa
     1321 attagaaggt gtcagttacg atgttcgcgg tcccgttcta gaagaagcgg agcggatgca
     1381 agaagagggc attagtattt taaaattgaa cacgggaaat cccgcacctt ttggatttga
     1441 agcaccaaac gaagtcattc gtgatttgat catgaatgcc cgtgactcag aaggctactc
     1501 agattccaag gggatctttt ctgctagaaa agcaattgag caatattgcc aactgaaaca
     1561 ttttccgaat gtgacgatca atgatatcta caccggcaac ggagtgagcg aattgatcac
     1621 gatgtgtatg caggggttgc tggacaatgg agatgaagta ctggttccaa tgccggacta
     1681 tccgttgtgg acggcttcta ttgcattagc tggaggcaca cctgttcatt atatttgcga
     1741 tgaagaggca gaatggtatc ctgatattga tgatatcaaa tcaaaaataa cgagtcggac
     1801 aaaagctatt gttatcatta atcctaataa tcctactggg gcactttatc caaaagaatt
     1861 attagaagaa atcgttgaag tagctcgaca aaataacctg attatttatt ccgacgaaat
     1921 ctatgatcgc ttagtgatgg atggtctaga acatattccg attgctacat tagcgcctga
     1981 tttgttcgtt gttacgctga acggtctatc caaatctcat cgtgtagcag gatttcgagt
     2041 aggctggatg gtcctaagtg gagataagtc acacgtcaaa ggctatatag aagggctgaa
     2101 tatgttgtcc tccatgcgtt tgtgttccaa tgtattgtct cagcaaatta tccaaaccgc
     2161 gctaggcggt tatcaaagtg tcgatgagct gctcctgcca ggcggaagga tctatgaaca
     2221 aagagaatat atctataatg caatcaatga tattcctggt ctttcagccg taaaaccaaa
     2281 agctgctttt tatatattcc caaaaattga tacaaagcga ttcaatattt tggatgatga
     2341 aaaattcgtt ttggactttt tacatgaaca tcatattcta cttgtacatg gtggtggatt
     2401 taattggaac cagccagatc attttcgaat cgtttatctt cctaaaatgg atgatttgaa
     2461 acaaacagct aagaaaatga gagaattcct agcaacctat cgccaaaaat gattttaaaa
     2521 accaaatttt tcagtaggag ggccacagac aaacacactg aggctctctt tctagtggag
     2581 ttttcttaaa taactaaagt atattattta gttgcaagtc ttttcttgaa gtttgcgact
     2641 tcctaaagta aaatacgaat agaatgcgaa tacgcatatt ttggggagga tacttatgga
     2701 aacgttaatc gagtcctttg gaacgatcat taaagagatt cgaaaagaac aaaaaatgac
     2761 gcaaaaaatg ctttcgcatg atatttgttc acaaagtgta ttaagtcgga tcgaaaataa
     2821 tgaggaactg ccgaatgtat tggtgatgat gcagatttgt caacggctag gggtaacgat
     2881 tgatcatgtg atgaatctct ctcgaaaaac tactcggaca aacgataaat tatttgaact
     2941 gttggatagt tgtttccaaa aacgtgatta tcggaagtta gaacaagtac taaaatcttc
     3001 tgatatttct gaaaatctgt atcaagcaac tgactttcaa cgctattatt attatctcgg
     3061 tgtttgcgaa ttcacgttgc gtcaaaatat ctctcagtcc ctttattatt tgaaagaagc
     3121 attaagctac tctagccaaa aagaaagaat ccatgtttct gacacagaaa tacaactgat
     3181 tagttgtatc ggaaaaatat atggtgtggc aggaaaaacg gctgaagcac gctactattt
     3241 gagtcgaagc atccaactgc tgcatcaatc agctgatgaa cgatcaaaaa gtgaattttc
     3301 gcaaattttt tataattatg gaaatttctt attccatcaa aatgaaatcg acgaagcgtt
     3361 agaacaggtg aatcaaggaa tctattgggc tcaagagaag aatagctact attacctcaa
     3421 tgatttattt gtgcttaaaa gcttaatata caagcgtaaa gaagaaccag aaagagcagc
     3481 attctatgaa ggcttagcac aagctgtgaa acaaatttca aaaaatttgt aaatggtgta
     3541 gaccattctt gacttcttct ctaaataaga gtactttaga ggtagaaaaa caaagggaga
     3601 gaatagaatg agaactttta tctatgctga caaattcttt ttagcgtcag gtgtcaaaca
     3661 tgcaggctat cttgaaatta ccgatggact ttttggtgaa tacaccaaag aactacctga
     3721 gggagaagtc aacatcattg accaaagcgg caaatggatt gctcctggtt tggtagatac
     3781 acatattcac ggctatatga atcatgatgt aatggataat gatgcagaag gcatcaaagt
     3841 gatgtctgaa ggtctgttat cttgtggagt gacttcattt ttaccaacaa cattgacgtc
     3901 aagcaaagaa cgtttaaaag atgtagctga gacaatcggc aaaatgtatc aggaagcacc
     3961 aggagctaaa atcaaaggaa tctattttga aggtcctttc ttcacagaag aacacaaagg
     4021 ggcacaaaac ccaagttatt tcggggatcc tgatttggat actttccatg aatggcaaga
     4081 agcttcaggt ggtttaatca aaaaaattgc tttagcaccg gaacgtcaag gtgtagaaga
     4141 gtttgtcaaa caagtgactg aagaaggagt agttgtttct ctaggacaca gtaatgctac
     4201 actagaagaa gcgcaaaaag ctgttgaagc aggcgcaagt gtatttgtcc atgctttcaa
     4261 tggtatgcgt ggattgaatc atcgcgaacc aggtatggtg ggtgcgttat tatctttaga
     4321 acatgtcttt tctgaattga tttgtgatgg ccatcatgta catccaaatg ctgcgggaat
     4381 cctaatggaa aaagcaggac atgatcatgt cgcattaatt acagactgta tgatggccgg
     4441 tgggatgcct gatggaaatt acaatttagg cgaatttcca gtagtagtaa aagaaggaac
     4501 agcacgtcta gaatctggca acttagctgg aagtatattg aaattgaaag aagcaattaa
     4561 aaatgttgta gactggggat tagcaacacc agaacaagca gtcatgatgg ctagtctgat
     4621 tccagcaatc agctgtaaaa tcgatgatac atgtggaaaa atcgctcaag gacgcgatgc
     4681 agactttatc gtattgaacc ctgatatgac attggaagca acttatctag atggcgtgga
     4741 acgtttccac gcataatata atttaagtta accttaaaac taaacttctt aagtttggtt
     4801 ttaaggtttt ttttgtgttc tagtaaaaat cactttacat aagtttcaaa agtttcttta
     4861 atttcctatt gttcaataag taacaatgta aagataaaat taaaaatgtt aatacgtatt
     4921 agcaaaataa aagctttttt tattgtgaat aaatgaacat aattaggagg agaaagatga
     4981 acttatttga catttttcga tcgactttga cagatatgtc gattgtcagt gcaatcactt
     5041 caacagtttt tattatatta ttagggtttg tttgtagaaa aaaggggatt ttttctgaat
     5101 ctgttggaaa aattctatcg aaagtcgtct taacagtggc tttgccagca ctggcatttc
     5161 gagctttcat gcaagatatc aatacagaat tactgatgca aggaatcaat gtcttgattt
     5221 ggggattagt tatttatatt attcttattc tagtttcaaa accttttttc ttaagctata
     5281 aaggggataa gcaaacaacg ttgcgtgtcc tgacgatctt tggttctacg acctttttcg
     5341 gaacgcctat cgtcagtgcg atttatggtc ctgtcggcgt gatgtattcc tcaattttca
     5401 atattggcta ccgaatcttt ttgtattctt atggatacat aaaaatgagt ggcttgaaga
     5461 tggaagctaa aaatatcaaa gcgatgttcg tcaatccaat cattatcgct acttttgccg
     5521 gccttttcat ttggctgttc caagggtatc tgccacaagt gtcagttaca ggtgcagaag
     5581 gagagataag ccaagtagcg tttttgcgaa ttgaccagac tgcaccgtgg ctatttcagc
     5641 cgatggatta tttagctggt ttaagttcac cattggcgtg gctagcaatc ggcgcaacat
     5701 taggtgaagt aagcttcaaa aaagctgcat ctgaaaaaac ttcatggtat tacagtctag
     5761 tcaaagtggt atttgtacct gcattgaata tcgtgattct agcagttttg acggcaacga
     5821 atattctacc agtcaatttt gaagcactgg caacaatcgt tatcatgatg gctacaccag
     5881 ctgcaacagt ggcagcagcc tatgctatca gttttgatag ggaggcactt ttagcctcca
     5941 atgcatcctt actgtcaact atttttgctg tggtactgac acctgtatgg atcgttgtat
     6001 tacaaatgat tcatggcata ggtttatttt cataagaaat ggaggaagaa agtatgagaa
     6061 tcgcatgtta cggtgttcgt ccgaacgaga tagatttttt tgaaaaattg aatatttacc
     6121 attatgatct cagtctttat gaagaactgt tgacacatga caatatcgtg tcagcaaagg
     6181 accatgatgc tgtattgttg aggggaaatt gtatagcaga tgagaccaat cttgcaaaaa
     6241 tgcaggaata tgggattcgt tatgttttta cacggactgt gggagtcaat catatcgatc
     6301 tacaagcggc agctgatttt ggtatgacag tagcccgtgt accatcgtat tcgccaaatg
     6361 cgatagcaga gttatctttg acttttgcca tgatgctttt gagaaatact gcgtatacga
     6421 cgatccgaac atctttcaaa gattttcgag tggatgagca aatgtttagt cgtgaaatca
     6481 gaaattgcac agtagggatc ataggaacag gcaggatagg gttgacggaa gctgttttgt
     6541 ttaagggact aggagccaaa gtgatcggct acgatattca tcagacagaa gctgctaaaa
     6601 aagtattgac gttccactcg cttgaagagt tattagccaa aagcgatatt gtcagtattc
     6661 atgtacctca tatcccaggt gaaaacgatc aaatgatcaa tgaaggattt ttagctcaaa
     6721 tgaaaaaagg tagcatcttg attaatacag cccgtggaga gcttcaagat aatgaagcta
     6781 ttttaagagc tttgaaaagc aatcatttag ctggttttgc aaccgatgtt tttgcaaatg
     6841 aaacagaggt ttttttccga tccttcaagc catgggagac gattccagac cctgctatcc
     6901 agcaattagt agaaatgtac cctagagttc ttgtaacccc gcatgtaggt tcaaatacag
     6961 atgaggcatt atcaaatatg atttcgacaa gtttcgagaa ctttcgtgaa ataatagaaa
     7021 ctggaaaaac gaaaaatgag gtatctcttc ctaaagcccg tcaattaaga taagaaaaca
     7081 tggatattcg tcaataaaat gtttaaaaat agtccaagta aacagttgtt acttctgtat
     7141 acttggacta ttttgtatgg gataattcga tgactcaaca gtaatctgtg agctttttcc
     7201 gcccattctt tgtcaattca taacaggtta gcggccggcc gatttccaaa tatttgattc
     7261 tttcgttcaa tatatcttgt tctactaaaa aattcaaata tttcttgacg gatatacgtg
     7321 acagtgagct tttttgagcc agttcttttg ttgaaaaaat agtatcttct tttagaataa
     7381 atgcacttat tttttttaaa gtcagttttg acagaccttt aggcaaatcg atgaatgatg
     7441 gtatttcgtg aatgggagtg gtgtggttga atacttgatc gatcaatttc tgatcggctt
     7501 tttcagtcgt ttggattgtc tgagagaaag caagatactt ttcgatcgcc agctgaaaac
     7561 gttgcaaagt gaacggtttg atcaaataat ctaaggcacc gtagcggatc acattcttta
     7621 tggtttcgat atcatctgca gcagtgatca aaatcactgg gatatcattt ccttgctgtc
     7681 taagccaaga taaaaaatgg ataccacttt cgccaggcat gtatacatca agcagcagca
     7741 gatctgcgtt tttttgtctg aaatattttt ttccttcttc tacatttctg aaactttcga
     7801 ttacgttcat attagggatg ctttccacaa aagtacgatt caattctgca accattggat
     7861 catcttcgat tatcaggaca tttgtcatca ttttgatctt ccttttttgg taattctaga
     7921 tagacgattg taccgccatt tgtttgtttg gaaaattcta aaaatccacc gtggcgtttg
     7981 acgattgaat ggatgagatt caatccaaac ccacgattga ttcccttcgt cgaaaaacct
     8041 agcgtaaata ttttttctga gatttcttct gatatcccga caccgtcatc gcagacggtc
     8101 acgatgaata cttcagcttc tgtgtcatag tttaaatgaa tccggacctg tccgtttctt
     8161 tcatgttttt gtagcacggc ttcttttgca ttatcaagta agttccctaa cacttgcaaa
     8221 agtgaatgga tggagtcatc catctccatt ttagggataa aagaatcgct ttctaacagc
     8281 atttcaacag attgttcttt ggcttcgttt attttgccta ataaaaaacc ggcaattgca
     8341 ggtactttga tcagacttga gacttggcca atctcttttt tataattttg atttagctgc
     8401 tggataaaat gggtgacttc ttcatacttc ttcaaatcaa tcaaaccaga aatgacatga
     8461 agtttattca tgaattcatg ggtttgagct ctcatggagt ttatatattg tttcgtcccc
     8521 ccaagctcct ccattaattt cattacttct gatcgatttc gtaaagttgc gactgctcct
     8581 aaaaaacggt cattgctaaa aagaggtgta acgctagaaa tagtttctat cccatttaaa
     8641 tacatggatt gagtccgaac ggtcgtttga tgctcaatcg tctctttaaa caaaacgtca
     8701 tatagattat cactgatttt tttcctactt cgaaatccaa tagctgctct gtttggtaga
     8761 aaatgttttt tgcaggtgta ttgatcaaga ggatagtttt atcagaagag atggctaaga
     8821 ttccttcatc tacttcattt tcgataatcg ttttttcact taactgttct gctatttctt
     8881 gtggttcaag tccaagcagt acttttttca ttttttgggc aagaaaaacg gcgcctatga
     8941 tgcctacgct aagtccaata atcaatgaaa aaacaatcat tttttgtcca tctttgactt
     9001 gattgtcaat cgtgttgttt gttaagccaa cacaaactgc accaataatc tcttgatcat
     9061 aataaatagg gacgaaatag cggaagccga accctaacat tccttctttt tcagagaaaa
     9121 attcttgtcc tccaatcact tttttcacat cttcatgact tgagaaagga agaccgatgt
     9181 aacttgggtc tgcgtgagaa tatcgaatgc cttcagggtc catgacaacg ataaaatcta
     9241 aattcgatgc aatcatgatt tcatttgtta ttccttgaat cgcatgtgtc gggtcaccag
     9301 tctgcagctt tttttggatg agtttatcat tggcaacgat tttagcaatg ctagagattt
     9361 tatctgacac actagatcgg ttcatcgaaa gaataaaatt cctgatcaat atggcagaaa
     9421 caatcaaaga aacgaatgtg gagaaacaaa cgacaaggat gatcgtgttc tggagcttta
     9481 atggtttttt cttaaaaaaa ggaaacatag gctacctcga aagaatttat tgtgaaaatt
     9541 ttaacatgaa aacaaaaatt taacaaattg tcaacgataa acacaacctt cctataaaaa
     9601 acaccactga gtcggtaaaa gaagctatac agaattttca aacattggta tgatagacgt
     9661 agaaatggaa ggaggcgtgt atatgatagg gggaatcgca cttgagacga tttattggta
     9721 tacattgctg atttgtgcag gattggcagt cctattgatt atttttggag atgtattcga
     9781 ttttgatggc ccgcttgatc cgatgcttat tatcccttgg ttaactttca caagcttatt
     9841 tggttttcta ggtgagcgtt taacagatca atcatcttta tttttattta ttgctagtgg
     9901 aggcatatcg atagtgttgg tatttttgct taatttctat atattgatgc cgatgaaaca
     9961 tgccgaatca acgatgtcgt cttctgaaaa aactctggaa ggacaagtag ctgttgtgat
    10021 cacaccgatc cctgtacgag gtatgggaga aattcagttg aaaagtgtta ccggttccat
    10081 tagtcgtcca gcttgttttt acagccccca agaacagaca gctgagcggg gaacgaaagt
    10141 attgatcatt gagatcaagg aaagaatctg ttatgtcatg ccttatgaaa gcatgataaa
    10201 agcttaaagg agcgtgtgtt aaatggaaat accagttgtt tttatcgcga tcgcaatcgc
    10261 agctttgatt ttactgatgt tgctgatcgt ctttgtagcg aagtaccaga cagcaaaacc
    10321 ggatgaagcg ttgatcatca gtggaagtta tttaggaagc aaaaatgtgc acattgatga
    10381 aagcaataac aaaatcaaaa ttgtgcgtgg aggtggcgca ttcgtcttgc cagtttttca
    10441 acgttcgaat cggatcagtc tattatcgag taaattggac gtctccaccc ctgaagtgta
    10501 tacggaacaa ggcgtaccgg tgatgtgtga tggtacttcg atcatcaaaa tcggttcatc
    10561 tgtagaagag attgctacag cagcagaaca gtttttaggc aagacaagag aagaactgga
    10621 aaatgaagca agagaagtat tagaaggaca cttgcgctcg attcttggtt ctatgactgt
    10681 agaagaaatc tatcaaaatc gcgataagtt cagtcaaagc gtacaagaag tcgcttctgt
    10741 agaccttgca aaaatggggt tgatcatcgt ttctttcact atcaaagagg tcagagacaa
    10801 aaacggttat cttgactctt tagggaaacc aagaatcgcc caagtcaaaa gagatgcaga
    10861 tatagcagaa gcggaagctc taaaagaaac gcgtatcaaa aaagctcaag cagaaaaaga
    10921 atcacaagca gctgaattac agcgacaaac agagatcgca gaatcattaa aagaaaaaga
    10981 actgaaatta gcaacctaca aacaagaaca agatgttgct aaagcaaaag cagaccaagc
    11041 ctacaattta gaaagtgccc gagcacaaca acaagtaatc gaacaggaaa tgcagattaa
    11101 aatcgtagaa cgtcaaaaac aaatcgaact ggaagaaaaa gaaatcacac gtcgtgaaaa
    11161 gcaatatgat tcggaagtga agaaaaaagc agatgccgat cggtacgcta aagaacaaga
    11221 agcacaagct caaaaagtca aagaagtgac tgaagccgaa gcagaacgat tccgtgtgga
    11281 agcactggca gaagcggaag ccaataagac acgtttagct ggacaagcag aagccgaagc
    11341 agcactagca aaaggaaaag cagaagccga agcaaaacaa aagatcgcca atgccttcaa
    11401 ggaatatggc gaagcagctg tcttgagtat ggttatcgat atgctaccgc aattgatgcg
    11461 cgaagcagca cagccgctag gaaatatcga aaaaatctct gttgtcgata caggaagcag
    11521 ttcaggagaa accagtggag cgaaccgagt gacaaattat gccacaaatt tgctttcttc
    11581 tactcaagaa acactaaaag cgacaacagg acttgacttg aaggaaatcg tagagaattt
    11641 gtcgacacgt gggactacta gccaagtaaa tatgtatgaa gcgccaaaag aggcagaaca
    11701 aaactcgatg aaggaagaat aaagtagtac acttcaccgt tcaatcaaaa atggaggaag
    11761 actttttaaa gttcttctct ccattttttt attttattgc aaaagaaaat tagtcctcgc
    11821 tatttttctt ttgtttttgg atcttagctt ctatctcttg taaatttgtt cttagcaatc
    11881 gttctttctt ttggagttcg accaaatctt ctgatagttc ttccttcttt tctgtttcag
    11941 gaggacgaag aaagaaattg ataccgattg caatgaatgt ccctataaaa gtatctagta
    12001 cacgttggat agcatacaaa gcggattcgc cttgagggat gctcaacgca atcagcaaca
    12061 acgtagctat tgcggaaatg ataccggaat tattgttaat accatctgac acgacgatca
    12121 caataataac tagaatagga agcaagatca attctaccaa aaaatcattg tgaaaatagc
    12181 ttttgacaaa aaaataaatg atagctaatg taccacctaa tgtattgccg atgatcctag
    12241 actttccaaa agaaacggtt gtagtcaagt cctgacgcaa agaaaaaaca gcagataatg
    12301 cagcaatcat cgggattcct cgttgagtca aatgaaaaaa caagatacat aaaaaaacag
    12361 ctaaggcact tttgatcgta cgcataccta gtcgaaaacg gccaaattgc atagagattc
    12421 tcctttttgg tctttttact atcataccta aacttttgtc tgatagctag tttttcatta
    12481 ttcaagtata attaaaataa aaaaggggga agtaaatgcg tataggaatg cgaacagtca
    12541 aaacagtgat cagtgtcttc ttttcgatgg tcacagcggg tagcttatcg ctgttgtatt
    12601 ggccagctgc agggatcatt gcattattaa gtgtaggaaa taccaagcgc tctacattgc
    12661 gtacgggcat gaatcgaatc gcagccttta tattagctac ggcaacagca ctgttcagtt
    12721 tttttgctgt tgggtatact atttttggct tcagtctttt tttattcctg ttcatcccgc
    12781 ttgcctctcg cttcaaatta accgaaggga tagtagtaaa ttcggttttg gtcactcatt
    12841 atttagtgga ggaaaacatg tcttggcagc tgatcggcaa tgaagcggga ttaatggcga
    12901 ttggattagt atttgctttg cttgcaaatg tttacatgcc agatcgtacg aagcagctga
    12961 aggaaaacca aatacagatc gaaaaagaat ttcgaaatat tttgagacaa atggcagaat
    13021 ttcttttatc agaaaataaa gaagatgtgc agatcaaatg cgaacattta ctgacattca
    13081 ttcgcgaaag ccaagaagat gccagagaac atcaagaaaa ctactggctc agacagccgc
    13141 tatactacga aacttacttc tctatgagaa gagcacaggc caatgtgatt aaagacatgc
    13201 tggaaaatct agagagaatc cagcaaccag cttattatgg aaaacatatc tatggtttat
    13261 tgatttatac tgcagaaaca ttttcagagt caaatgatgg cagacaaatt ttaacacgta
    13321 ttgaagaagt ttatgtattg tatcggcaaa tgcctctgcc gactagtcgc cctgaattcg
    13381 aggatcgagc agaattgttc cagtttttgc agtcttttaa aagttttatt gaaatcaagg
    13441 cagaattctc ccaacaaaaa atccaaaaat agaggttgtg aattcagcta atattcaggt
    13501 caaaaagctg atttcacaac ctcttcttta aacggtgttc tcctgtcaac tcctcggtca
    13561 caagtcacaa tattcaacaa acatgagaag ctgttttttg aaattgctcc ttgtgactcg
    13621 gagctgaacg gtaggctcac aacctctttc ttaaacggtg ttcaaaagct ttcctctgcg
    13681 gtaataagtc cagccaaacg aaaaatagga ggagctattt ttgtttggct gttcttatta
    13741 cttgatgcag aacagctttt tcacaacctt ttaaaattac atatttttcc atagaacttg
    13801 catttgatcc aagatttcga tgacatcttt tgtcaagaag agagagcgat tgggcaagtc
    13861 accttcaatc atgtgtacca tattctttat ttcgtaattc attgcttcag atgtattccc
    13921 gctttcgatg aattcctttg taccatctgt aaataagatc tccgcacgat cagcacgcgg
    13981 gtaatcagca atcgtcacat aaccgttctc aaaggcaacg ataccgactt ttggcatttt
    14041 tgcctgaaaa gtcaagctga cagcagcaag ttcattttct ttgttacgca agatagtgac
    14101 ggattgttcg tccacgcctg tttcaaaagg aaccattgta gaggcaacta cttcaggttg
    14161 agagctcaaa aagaaacgag cgaaagatac agcatacgta ccgatatcca acagagctcc
    14221 tccagctaat tctggattga agaaccggtt tttagggtct ggttctttgt aactgccgaa
    14281 aggtgcttgg atcattttta aagcccccag ttttcctgta tccattattg agcgtaactg
    14341 ctgataaagt ggcatgttga agattgtcat cgcttctgct aagatcaaat tcttttcttc
    14401 tgctaagcgc atagcgtcag ccagttcttt tttattcatc gtgatggctt tttcacatag
    14461 aacgtgtttg cctgcttcca atgctgccaa gatatggtcg atatgctgtc tatttggtac
    14521 agcaatgtac acgatgtcga tttcagcatc gtctaacatt tcttgatagc ttccgtaagc
    14581 ttttggaata ttgtaacggt gagcgaagtt ttccgctttt tctgaagttc tggaagcaac
    14641 tgctgttagt tcgctcgtct cttgattgaa gctttcggca aactcatgtg cgatggtacc
    14701 aagaccaacg attccccatt gatatttttt catctgaaaa cgctccttca tagttggatt
    14761 gagtttcttg tctgtctatt attataacaa aaatcacgaa gaataaagag gagaggactt
    14821 gaaacattaa aaaaaatgaa agcagcgatt taagaggtgc tttttgatca aaacttaagt
    14881 agaatagtta aaaggagggg ataaacgtgc atcaagtata tattgatctt attatgagtg
    14941 tattattaga acagtttgaa actgaaaaaa attttttaga tgaatacttg ctgatcagtc
    15001 cagaacaatg gcaaaaatgg aaagatggcg agaaaaatct gccatctgaa gcgatgcaga
    15061 aagtaaaaaa tcttttcact gattatgaat ggatgctaac gcaaaagatt ttgcgtcaaa
    15121 cgattatttt tcccgaaaaa cgaaacgttg ccgttgcaga gtacaagcaa atcaaaacac
    15181 gcatcgcgca aaaatggctt agcagcaaag cagcaaaagt cgagctgatc cctttgaaaa
    15241 ataatcagca ggctggtaga taccttgact tgaaagtaag tattcaatat gatgaatggg
    15301 gctatgatga tattttgaat tttcgtctgc cagcagatgt tcaaaaacag atcgaaggag
    15361 aaaaaatcga acttctcgat tgggtgaatg aaaatctgga agaaacttac gttcatgagg
    15421 cgaatgggga acgtgaagaa caacgaaagg atttttcaag tgaataaatt tttaggattt
    15481 ggagcagttg tgattatgat cgcagcaatg ggctatgtta tttttagcga tgatgattcg
    15541 catgttcaag ccggcaatca agcagtcgaa cgaacaagca ccaaaacaac tgctagtaaa
    15601 gaaaagaaca gtcaaacaac cagcagcaag gtaaaggaca aagaaacaaa agatcgtacg
    15661 tccaaacaat tgccagatgt taaaacagac gattggaagc tagttttggt tggtccagat
    15721 cacaaattag aaaaagaaat tgatgagtct actcaattgg catcattaag taacggatac
    15781 ttgatcgaca aacgcattgc tccagaatat gaagcatttg ccaaggctgc tgaagcagct
    15841 ggatttccat tagtgatggt ctcagcttac cggtctgtta tcatcccaac aacaagtttt
    15901 cgaacaaaat gtccaagatg tgatgagtcg tcaaggtgta tcagaagagg aggcgacaaa
    15961 gattactaaa gaaacgatta cggagccagg atacagcgaa caccacacag gtctggcagt
    16021 cgatgtcgtc gatcaaaact ggtacaacag ttatccttct actgtattgg atgcaagtta
    16081 cggagatcag cctggagcta aatggatcgc agataatgct gcgcagtatg gatttatcgt
    16141 tcgttatcca aaaggacacg aagatataac taagatcaca tatgaacctt ggcacttacg
    16201 ttatgtcggt aaagaaaacg ctgagtatat caccaaacac caattgacgc tagaagaata
    16261 tctgaatgaa ttaaatgaaa agtaggaatt gatcatggcg aaaaaaaagt ggaaaaagca
    16321 aaagcgcaag aatatccgtt ggccagcagt ggttgccggt gtagcagtga tactcttagc
    16381 ctttgttttt tccttgaaga atctgtcttc tccttttact gataatcagc aagaaaatga
    16441 agcgcaatcc catcagcaat ttattgaacg gcttgttccg catgcccaag aactacaaga
    16501 tggttatgga gtgcttccta gtatcatttt aggacaagct atattagaat caaattgggg
    16561 aaaaagtacc ttggctagca aatacaataa tcttttcggg atcaaggctt atggggatca
    16621 gaagaaagta agcctggaaa caaaggaatt tgttaacgaa gaatggataa ctatccaagg
    16681 agacttcaaa gtgtatgatt catgggaaca gtccatggat gaccatacac aactgttcgt
    16741 ccaaggagta gattggaatc ctgcgctgta tgaaaaagtg attacggcca ccaactatca
    16801 ggaagccgca caagcattgc aagatgcagg ttatgcgaca gaccccggtt atgcgcaaaa
    16861 aatcatccag gtcatcgaga cgtatcaatt gaatcagtat gaccggagat agcaaaggag
    16921 aagtggctga tggcagaaca atttacgcca gaaatcatga cggaattgag aaaagatatc
    16981 gtaagagtac cggaaatcat tttagaagca agcgggatca agatatttgg aaaattgatc
    17041 cgttcgatca tctttactac ggatattgcg atcatacgta atacaaatgc gaatgcagta
    17101 attgctgtgt atccttttac acctcatcca gcgattacta aaagcattat tgaggcagct
    17161 gacattccag ttttctccgg tgtaggtggt ggtttgacac aaggtgctcg ttcttcctac
    17221 atgagcatgt tcgcagaagc gcaaggttcg ttaggggtcg ttttgaacgg tcctacgcct
    17281 ttagagacag taaaagaagt ttgtcgagta gttgatattc cagttatcag tacggtaacg
    17341 tcgaaatata cagaaataga tgaaaagtta gcagctggta tttcgattat caatatcagt
    17401 gcaggtaaag aaactgcaga agcagtacgc cattttcgta aaaaatatcc tgaacttcct
    17461 atcattgcga caggtggtcc aacggaagaa agtattgctg agaccattca agcaggagca
    17521 aatgccatca catatacgcc accaagtagc ggagagcttt ttagtcgtaa aatggaaaaa
    17581 tatcgggaaa ctgagaaaaa gaatcatgaa taacaggtga gaatcaaaag acgaggaaga
    17641 gcagatagtg gctcttcctc gtcttttgta ctccagtact atttgttcag aattcaaaaa
    17701 aaagagaaat ttttttgttt ttttacctgt aatgtacata agaagagtta aaaccttttg
    17761 ttattattag cattctaaca aaaaaatgtc ttgatttatt gaagcgcttt ctatagaatg
    17821 aaaaacgaat aaaaactaaa aaaggcaccc gacaatttag tagcagctaa attgtcgggg
    17881 cttgaaaagt cagcgggaac tgaccaatca agttgccata gtcaatgctt gtgcatcgac
    17941 gtgtatattg tacctaattt catacagaat tactagttat ttttctgttt tttagggaag
    18001 agcgcacacg aagaagcatg cttatttagc tatgtcaagg atattgggca gttgtctgat
    18061 atcctttttt ctttgcgtaa ttatcctccc atcattgaag caagaagtgt ttttagcgat
    18121 gaaagaagaa tcagcactat ctaatttttc ttgaaagtat tgagttgttc atcctaggca
    18181 gaacgataag gcaagtcttg gtttttattc cccttgaaaa agatcgttta ataaatatgt
    18241 tctgtataaa aatacagtat attgaaaaat aacaatggaa gtagaggtgc agagaatgaa
    18301 gaaaagaatg agaatcatct tagctttaag tgttatgtta ttcttcttga cggcttgtgg
    18361 gcaagaagat gacaaaaaaa tcacgctgaa catggcttgg tggggttcgc aagtccgaca
    18421 cgatgcaaca gtaaaagtaa tcgagcttta tgaagaaaaa aatccccatg tgacgatcga
    18481 ttatgaattt tatgattatg aaggatatta cacaaaatta ggtacactag cagcatcaga
    18541 tgatatctgg gacattttcc aactgaacaa tcaatttcct caatacatca atcagattga
    18601 accattagat gaatatgtgg agcaagggat aattgatatt tctaatgcag atgaatccta
    18661 tttagcaacg acgacttacg acgggcaatt agttggactg tctaacgggg tgaattcgtt
    18721 tgcgattgct tacaataaaa atatatttga acaattaaat atccctgaac cagcttcaaa
    18781 ttggacatgg gatgaatttg aacaaatcag ctatcaaatc actgaagaat taggaatcta
    18841 tgctatttca agattcgaag attttatagc tggctgtatt attcaaatac cacaagttga
    18901 gaaaggattg aatttttata atcgggaaga tctatctaac agcttgggat ttgaaaatcc
    18961 agactatcta acagattttt tccagctgcg taaaaatctg gtagacaaag gggcccatcc
    19021 agaaccaggt ggtggagctg caagtacgac ggatctgtct tttcaggcgg ttagggatag
    19081 agaagcggca atggtgtttc tatcaagtaa ccagttgaca gaaatattgt caggggctcc
    19141 tgaagatatg gagattcagt tgattaatcc acctcgcaga aaagcagatg gagaatctgg
    19201 tatcccttta cgttcttcgc aaatgctgag tatggcaaaa agctcagccc ataaagaaga
    19261 agctgctaag tttattgatt tctttcaaaa cagtgaagaa gccaatgaaa ttttaaaagg
    19321 agaacgtggt gtaccaatca tgtcgaatat tcgagaacag ctcgctcgtg cagatgatgc
    19381 aactttgcaa gcgacatttg attatttaga aatgatttcc gatatggaca acggcgaagt
    19441 gaatgtcttt gatagtgctt tgaatccgga aattcaagac caatacaatc ttttagttga
    19501 aaaagtcatt tatgaagaag taacagcaga agaagcagca gagagtcttt atgagtttgc
    19561 gtcaaacatt ctaggatagc agcaggagga aggcatgaaa aaagaaaaac taaaacggtt
    19621 cttggatcaa gacaatgtgg tcggttacgt atttgcttct ccattcatcc taggatttct
    19681 attgataaca gtgataccga tgatcttgtc actttattat tcgttcaccg attatcgttt
    19741 gggaaatcca gtctcatgga tcggcttgga taattatata aagatgctga gtgatgcccg
    19801 ttttttgaac tccatccgag tcacttttct ctatgtattt gcatctgtac ctgtgaaact
    19861 gatctttggt ctattgattg cttatctttt aacgaggaat atcaaaggaa cgactttctt
    19921 tcgttctttg tattatgtgc ctagtttgat cggtggcagt atttctatcg ctctagtttg
    19981 gcgggagttg ttttctaaaa acgggttagt gaatattttc ctggaacgat taggcgcacc
    20041 tgcgctttcc tggtttagcg atcaaaaact ggtcatgatc cctcttgttt tgatgtctgc
    20101 ttggcaattc ggttcggcaa tgatcatctt tgctgcaggt atcaaagaga tcccaggctc
    20161 gtattatgaa gcggcagaga tcgatggagc gaacaaatgg cagacgtttt acaagatcac
    20221 gttacctctt ctttcgccga tcattttgta taacttggtt atgcagacaa tcgcggcgtt
    20281 tatgactttt acgcaggcat ttatcattac ccaaggtggt ccgaatgatg ccacgaactt
    20341 ttatgctctt tatgtgtata accaagcttt ttcttttaat tctatggggt atgctagtgc
    20401 tatttcgtgg atcatgctgt tgattatggg agctgtcacc tttctgattt tcaaatcatc
    20461 aaaaaaatgg gtcgttgtgt gaatgagagg aggaagaaat gaataataca gtaacgaaat
    20521 ggtttcaata cggttttatt ttgctattag gtctagtcat gatttatccg gtcttatgga
    20581 tgatcgcagg ttcttttaaa acggatcaag agattatgag tggttcgctg aatttgattc
    20641 cagagacttt tcaatgggaa aattacgcaa aaggatgggc ggggtttgct ggaatttcat
    20701 tttttacttt ctttaggaat tcgtttgtca tctctgcagt ttcgacgata gggacagttc
    20761 tgagctcgac ttgtattgct tacgcattat cgagaatcga tttcaaagga aaaagattct
    20821 ggttcgtcgt catgttagta acgatgatga ttcctgcaca agtgattttg attccgcagt
    20881 ttattattta caatcggctg aatttggttg ggacgtatgt gccgctgatt ttaccacatt
    20941 tttttggaca agcatttttc atttaccaga tcatgcaatt catggtcagc atccccaaag
    21001 agttagatga atcagcgatt attgacggat gcagcaagta tagtgtcttt accaaaatta
    21061 tttttccttt gctgaaacct tcgatcatca cgacgatcat catccagttt tattggaaat
    21121 gggacgactt catgggacca ttgatttatt taaacaagcc gagatcttac acggtatcga
    21181 ttgccatcaa attatttgca gacgctggat ctagtacgac ttacgcgagt atgtttgcga
    21241 tgtctacgtt gtcgctgcta ccggtttttc tgattttctt attcttcaat aaatatcttg
    21301 tccaggggat cagtacaagc ggtctgaagg gctgaatatg aaaaagccaa tagaagagat
    21361 gtttctttac aagctcaact actatgtctg tcggattttt ttaaccagtc ttatcagtaa
    21421 tgctcttttc gctttagttc taggatgttt gttcacttta ggattagtgc tgtctgctcg
    21481 tgttgtctat ctgatttatt ttttgatgat gtatccttct tattatctat ctgtccatta
    21541 cttggcagaa aaaaaggtat gttggaggaa aggaaaaatc aaacggatgt gtttcatcat
    21601 tacgttgttt cagctgtttg tcattatttt tgcagttgat tggtatttat tagtgggaaa
    21661 gattggagct tggtatctga ttccttttct attgtgtctg ttgctttttg tatcccatag
    21721 tttttttagt acgctaattg atatctgcaa agaagataaa gcagatagtc ttgtttattt
    21781 gatcaaaagg aatatctggc tgttgtcaaa aaacgtcaag gggtttctgt ggtatttatg
    21841 ccttttgtat ggactgttct ggtgtataca gcatgatttt ttactcactg cattactgta
    21901 tccagggctg ccatttgtca cagtattttg cttaaaagaa aaggagaatg actttaaatg
    21961 aagaaaaaat atgtacaagt cggacttggt gggagagcgc gtttttttta tgaggcaatt
    22021 gcctctacgt atcatcaaac ttgtgaacta gtcggttttt gtgatgtatc tcaaacaagg
    22081 atggattatg caaatcggat acttgtgaac aagtatggac agaaaaaagt aaagacatgt
    22141 atgccatcac aatttgagca actactggat gacgtacggc cggctgaagt cattattacg
    22201 tcagtagatc gaacgcatca tgattatatc attcgtgcaa tgaaaaaagg atatgatgtt
    22261 atttgcgaaa aacctctgac tatcgatgag gaaaaagctc aagaaataat aacggtccaa
    22321 aaacagacgg gaagaagcct gcgagtcact tttaattatc gttatgcacc gtatcacacg
    22381 aagataaagg aactgataga aagtgatgtg attggtgatg tgttttctat ccacttcgaa
    22441 tggctgttaa atacgcaaca cggggcagat tatttcagaa gatggcatcg aaataagcac
    22501 aatagcggcg ggcttcttgt tcataaatct acccatcatt ttgatctagt gaatttttgg
    22561 ttgaacagcc gtccccaaaa ggtatttgca ttcggtggat taaactttta tgggatgcat
    22621 aatgcggaga aacgcggagt agaaatattt tacagtcgat gctatggaag cgaactagct
    22681 aaagctgatc catttgctat agacctgtca gccaatgagg aattgcgaga aatgtattta
    22741 gaagcagaaa aagacagtgg atatattcgt gaccgcagtg tattttccga tgatatctct
    22801 attgaagata cactaggcgt aacggtcaaa tatgaaactg gtgcgatttt gacgtattcg
    22861 ctaaacgcgt atatgccgtg ggaaggattc aatgtttctt tcaatggcag caaaggacgg
    22921 atcgaaatga aagtgatcga aaagtcttac atcaatgcag gtggagataa aaaggatgaa
    22981 ggagcagccg ttcttcagtc catccatgtc tatccgatgt tcggtgagcc atatgaggta
    23041 atcgtcgaac aagaagcggg cggacatggc ggaggagatc cgcgtttatt agatgatcta
    23101 tttggtgaag aaaaagaaga agatccgtgg aatcgggcag caactcacgt agatggtatt
    23161 ttgtcgattt tgacaggaat cgcagcaaat cattctattg cttcaggaaa agcagtagtg
    23221 attgacgagc ttgtcagctt aaaagaataa gaatagattt acggaaatta cttcccgtag
    23281 tttataaaat cttgccttat tgccgatagg ctgtcttttg aacactattt attcggaaaa
    23341 aggggctatg acatgctttt tgtcatgcct tctttttatg tgggctgctt ttttagatag
    23401 gagaaaataa aaataactat ttaaacccga acaaaaagga ataatgcatt aaaaatcatt
    23461 agattttaca aaaaaataag taaaccacgt tgtcagtttc agaaagtagt gttagactgt
    23521 tttcaatatt tgaaacaaca ttttttattc aaatatggca aactgccttc acgtgcaaaa
    23581 cagttgcaag aaaatgaatt tacactatta ttagttttac atacctactt atatatcgtc
    23641 ataatatgga tgacagtttc tagccagtac cgtaaatgct ggactataag taaaagattg
    23701 gctatttcac ccttcttttc ttatgataaa gtggagggtg tttttcttta acagaaacat
    23761 agtaggtttt tgcacaaaag cggttatttc ggtattaata aatgataaag gagcaaataa
    23821 agtgaaagaa ttagttgaac gtattcaaaa agacggacgt atattaggcg agggcgtatt
    23881 aaaagtcgac agcttcgtca ctcaccaagt agaccctgta ttgatggaac aaatgggaaa
    23941 acggtttgca gaagtattta aagaacaagg aatcacgaaa gttgtgacca ttgaagcttc
    24001 tgggatcgca ccagctttat ttgctgcaag agaattagat gttccaatga tcttcgctag
    24061 aaaagcaaaa agtttgacga tggatgaaga attattgaca gcttctgttt attcttttac
    24121 gaaacaagtg acaagtacga tttctatttc tcgtaaattt ttatcttcag aagacaaagt
    24181 attgattatc gacgatttct tggcaaacgg acaagcggca aaaggattga tcgaattatg
    24241 ccagcaagct ggagcgcaag tagaaggaat cgggatcgtc atcgaaaagt ctttccaaga
    24301 tggacgtcag ttacttgaag aaatgggatt gagagtcgtt tctcttgcgc gtattgcttc
    24361 cttgacgaat ggacaagttg agtttcttga ggaggacgca taatcgtgga aaaagaaaca
    24421 caaaatggaa aagctgcggt tttagggctg cagcatctgc tagcgatgta tgcaggagca
    24481 gtagcggtgc ctcttttgat cggtacagga ctaggcttta atcaagaaca gatgacttat
    24541 ttgatttcaa ttgatatctt tatgtgtgga ttagcgacat tattgcaatt gacagttaac
    24601 cgatttttcg gaattggctt gcctgtcgtt ttaggatgtg cgatccaagc agtagctcca
    24661 ctgatcttga tcggaaccaa tgaaggagta ggagctatct acggatctat tatcgcttct
    24721 gggatctttg ttgtgttagt ttctggtgtc ttttctaaaa ttaaaaaact attccctcct
    24781 atagtaacag gaacggttat caccgttatt ggtttgactt tgattcctgt agcaatcgag
    24841 aaaatgggtg gaggtaatgc aacagcagct gattttggtg acaaaacaaa tctgctactt
    24901 gcatttgtga cgattctcct aattgtaggc gtgcaaatgc tggcaaaagg gttcattcgt
    24961 tcaatcgcgg tattgattgg tttagtcgga ggaagcatct tagctgcttt catgggaatg
    25021 attgatctga gcgcgattgg agatgcgccg ttgttccatg ttccacaacc cttttatttt
    25081 ggtaaaccaa ctttcgacgt atggtcgatt ttactaatga tcatcatttc catggtcagc
    25141 atggtagaat caacaggtgt atattttgca cttggtgaca ttactgggaa aaatgttgga
    25201 gaagaagaat tgaaaaaggg atatcgtgca gaaggacttg ccgttatctt aggcggtatt
    25261 ttcaatacat tcccttatac tggcttttct caaaatgtcg gattggttca actttcggga
    25321 atcaaaacaa gaaaacccat ctatttctct gcattcttct tgattgtttt aggattattc
    25381 cctaaaatcg gtgcgttagc acaaatcatc cctgagccgg tacttggtgg aggaatgtta
    25441 gtcatgttcg ggatggtggc agtccaagga atccgtatgc tgttggaagt tgatttcagc
    25501 aatgataaaa atctattgat tgttgctgtt tctgttggat ttgggctggg tttcaatgtt
    25561 acgccgacat tattcagtca aatgccgcaa acattgcaaa tgttcactgg aaatggcatc
    25621 gtaatgagta gcattacggc tatcatattg aatttgatct ttaatggatt gaaaaaagat
    25681 gaagaaatat tggaagaagc cttcaagaag taatagagca aaagaaaggt tgtgaaatca
    25741 gcgttttgac ctgattgtta gagaaacaaa cggaaaaata gttcctcgta ttttgccgtt
    25801 tgtttgagct aataccgagg gtcagctgat tgaacaccgt tgaaggaaga ggtcgtgagc
    25861 ctgccgttca tctccgagtc acaaggaaca atttcaaaaa aaagcttctc atgtttgttg
    25921 aatattgttc cttgtgaccg aggagttggc aggagaacac cgtttatcca taaaaaggaa
    25981 tgtgacctaa cttttgtcac attccttttt tttatcttta ataaacacga acaataaaat
    26041 gtttttcttt gttaacattc gaaatttctt tgacatcaaa aaaagtgatt gttaaaatat
    26101 gaatgtagaa aaactgggag gaaaaacaat gacagcaaaa atctctgtga ttatggggag
    26161 tacttcagat tgggaaacaa tgaaagaagc atgtcaaata ttggatgaat tcggtgtgtc
    26221 ttatgaaaaa aaggtcgttt ctgctcatcg aaccccagat ttgatgtttg aatatgcaca
    26281 aaacgctcga aaaaaaggaa tcaaaatcat tattgcagga gcagggggag ccgcgcatct
    26341 gccgggtatg gtcgcatcaa aaacaacttt gccggttatc ggtgtaccgg tccaatcacg
    26401 aacattaaat ggtttggatt cgcttctttc gattgtccaa atgcctggag gagtacctgt
    26461 agctactaca gcaatcggaa aagcgggagc aattaatgct ggactacttg cgatacagat
    26521 gctttccatg tatgatttag aattggaggc taaattggct gaaagacgtt caatgttggc
    26581 taaaactgtt atagaaagta gtgatcaact tggttaaacc tttgttgcct ggttcaacga
    26641 tcgggatcgt cggtggcggt caattaggtc gtatgatggc gattagtgca aaagaaatgg
    26701 gctttaaagt aggtgtgctg gatcctgtga gcgattgtcc agccgcacaa gttgcagatt
    26761 ggcatattga aagtgtctat gatgacacct ttgcgctgaa agagcttgcc agaagaacag
    26821 atgtcattac ctatgaattc gaaaacgtca gtgtagaggc gttaaatgcg attttaccca
    26881 tgtcatttat tcctcaaggg acagacttgc ttgcgattac gcaagaccgc ctgttagaaa
    26941 aatctttttt ggaaacgaat aacatcgtga ttgctcctta tgcaacgatc gtcagtccga
    27001 cggatatcca agatgcgatt gacgggatcg gctatccttg tgttttgaaa accactcgag
    27061 gcggctatga tggcaagggg cagtatgttt tgaaaagtcg ggcggatcta gctcctgcca
    27121 tggacctttt aagagaagga acctgtgttt tagaagcatg gatcccgttt gaaaaagaga
    27181 tttcgatcat ggtggcagga aatggtcaag gagatttcac gacttttccg gtcgtggaaa
    27241 acatccacca caacaatatt cttcatgaaa cgattgctcc agccgcgatt gatcaggatg
    27301 tgatcgagga ggctgagcgt attgcccgtg tgattgcaga agcagtgagc ctttcaggtg
    27361 ttttaggggt agaaatgttt ttaacaaaaa caggcgggct atatgtgaat gaattagctc
    27421 ctcgtcctca caattctgga cattattcta ttgaagcttg ttctatgagt cagttcgatg
    27481 cccatattcg aggtatttgt ggttggccat taggagaaaa agtacgtttg ataagcaatg
    27541 cagtaatggt caatgttttg ggagaacaac tgctggaaag ctatcgcttg attgagaata
    27601 agccagactg gcaattccat ttctatggaa aaaaacaagc aaaaaaaggt cgcaagatgg
    27661 gacatatcac tatcttgaca gaagacatcc agcaaacgct ggaagaaatc aaacaaacga
    27721 atatttggga ttagaggaga aaaaacatgt tagaacgtta tacacgccct gaaatgggga
    27781 atatctggac ggaagagaac aaataccgtg cgtggctcga agtcgagatt ttagcagatg
    27841 aagcttgggc agaacttggt gaaatcccgc aagaagatgt gaaaaaaatt cgtgagcatg
    27901 catcatttga tatcgatcgg atcttggaaa tcgaaaaatt aacacgccat gatgtcgttg
    27961 cgtttacacg agcggtttct gaaacattgg gagaagaaag aaaatgggtc cattatggtc
    28021 taacaagtac ggatgtcgta gatactgctt atggctatct catcaagcaa gcaaatgata
    28081 ttttacggga agacttgaaa cgttttacgg aaatcattgg cgaaaaagca aaagaacaca
    28141 aatatacagt gatgatgggt cggacacacg gtgtccatgc tgaaccaact acttttggat
    28201 tgaaacttgc cacatggtat tcagaaatga aacggaacat cgaacgattt gaacatgcgg
    28261 caaaaggagt agaagcagga aaaatctcag gagcagtggg aacgttcgca aatatcccgc
    28321 catttattga agagtatgta tgtaaacaat tggggatccg tccacaagaa atttctactc
    28381 aagttctccc tcgggatcta catgctgaat atttctcagc gatggcgctt attgctacaa
    28441 gtattgaacg ttttgcaaca gagatacgcg gattgcaaaa atccgaaaca cgtgaagtag
    28501 aagaattttt tgcgaaaggt caaaaaggtt cttctgctat gccgcacaaa aggaatccga
    28561 tcggttcaga aaatatggct ggtctagcca gagtggtgcg cggacacatg gtcactgcct
    28621 atgaaaatgt cacattatgg catgaaagag atatttctca ttcttcagca gaacgaatca
    28681 tcttgcctga taccacgatt ttattgaatt acatgttaaa tcgttttggc aatatcgtta
    28741 aaaatctaac ggtctttcct gaaaacatga agcggaatat gtctgcaaca tttggtttga
    28801 tttatagcca gcgcgttatg ctgaaattga tcgataaggg aatgacaaga gaagaagctt
    28861 atgatcttgt tcagccaaaa actgcgtacg cttgggacaa ccaagtgcag ttccgcccgc
    28921 tgttagaaga agatccaacc atcacatcgg tattatctaa agaagaaata gacgatgcct
    28981 ttgattatca tcatcatttg aaaaacgttg acgtgatatt caaacgagta ggtttggaat
    29041 aaagaagagt gagttgcaaa attcaattaa aatagaatag caaaaataac caaaaaagaa
    29101 gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc aataatagga
    29161 acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa aaatagagga
    29221 attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga tgttgcagca
    29281 agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg atacgataat
    29341 gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct ttgtgttctg
    29401 tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct cggcggattt
    29461 ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg atcgacagca
    29521 aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa agctgacctt
    29581 gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa atttttagtt
    29641 aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta taagattttc
    29701 gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca tatttttgtt
    29761 gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa aacgatgtat
    29821 ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac ttttgtatga
    29881 aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga tagaatattt
    29941 agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa aaggagaatt
    30001 gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa taaaaaatca
    30061 ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa ttatccctct
    30121 ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag gtgttcccga
    30181 aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc agttggatga
    30241 tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg aagaaatcag
    30301 tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat ttgctaaggt
    30361 cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac aaattttgct
    30421 tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga atgaacatat
    30481 ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc aagaagtata
    30541 taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt cgtaaaagta
    30601 tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa aaaagcagtc
    30661 catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt tgagatacaa
    30721 cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga caagctgctt
    30781 gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga agtataacat
    30841 gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt tatgggcagt
    30901 tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct ctttagaagg
    30961 atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga gatgcggtgc
    31021 gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag aagggaaacc
    31081 agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt tgccaggtgt
    31141 actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga aagtcaaccc
    31201 caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc cagttgctca
    31261 tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa aaaataatca
    31321 gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg cagggattac
    31381 gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag tagaagactt
    31441 gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact tcggaaaggt
    31501 gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga aaaaaagaat
    31561 ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag atatcttagg
    31621 aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg aacattgttc
    31681 ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc atgtgttgca
    31741 aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag ttgtcttcaa
    31801 agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg cgacaggagt
    31861 tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt tgctagattc
    31921 attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag aagtagttgc
    31981 aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg aaattgcatt
    32041 tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt tgattgaaca
    32101 caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt atgtaggtgc
    32161 aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat ttaatcaaga
    32221 agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa agctgctgct
    32281 tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta tacaggatat
    32341 gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg gctcaggact
    32401 tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt atgaaatgat
    32461 gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg aagaaaaaat
    32521 ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag taacagacga
    32581 cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag tcgatgcatt
    32641 agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa tcgtagaatt
    32701 tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat tgcttgatct
    32761 gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg attcacaagt
    32821 acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg ttagagggac
    32881 gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt tggatccaaa
    32941 aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta gcggtggtaa
    33001 gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag aagttttttg
    33061 ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag gaactcctgt
    33121 tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct atccaacacc
    33181 aatgattggt atggtcggat tgattgagga tcataaacat atcactactc aggaattcaa
    33241 aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg atggcagtga
    33301 attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg atctatccat
    33361 tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga ttgaaagtgc
    33421 tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt tcaaacatca
    33481 gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag aaactcaatc
    33541 aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa tgatgggaga
    33601 tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt cagcaactga
    33661 cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg caatcgtatg
    33721 tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg gggtcatcca
    33781 gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg acaagaaggt
    33841 gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt ggggctgctt
    33901 tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc tgcaatcgga
    33961 catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc ttttctattc
    34021 aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa tgctcgttcc
    34081 cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga tacggagata
    34141 ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt aaaaagtgct
    34201 ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc aatgattgcc
    34261 gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa cggagcatac
    34321 gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat aagagatgtc
    34381 cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg ctttacgaag
    34441 gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc agattcaaac
    34501 atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt agcgaaagaa
    34561 tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc agccgcaagc
    34621 ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa tcaatacatt
    34681 gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg aatgaaatta
    34741 tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga ttcaattgtt
    34801 cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc aaaagaagtc
    34861 catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat tgatattcaa
    34921 acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga atgtatcggt
    34981 gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg tctggaaaaa
    35041 gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc gacgccgtta
    35101 tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt cttttaaaag
    35161 cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag ctggcgtaga
    35221 tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa aaacggaacg
    35281 tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat cggtctttca
    35341 attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat tagcagtggc
    35401 aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt gtgtcaatga
    35461 tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa cggggaaaaa
    35521 tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg ttcaaggaaa
    35581 tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag gtaatgatta
    35641 tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga caggagaaac
    35701 gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt caaatgggta
    35761 ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat caaaactgcc
    35821 agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct atgtttcttc
    35881 cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca ctggtggagg
    35941 gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa tcaaacttgg
    36001 ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa ttcctgagat
    36061 ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcg aagaatcgaa
    36121 agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg aaatcgggaa
    36181 agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat gagaatcgct
    36241 gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct atcgaaaaaa
    36301 ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata tgttttgaaa
    36361 cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt tgattcaaaa
    36421 aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga tttgatcgta
    36481 ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga taaaagaatc
    36541 atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat acgtgatgcc
    36601 tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca aggtgtagat
    36661 actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac gttcgattct
    36721 ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat tagtgaaatt
    36781 attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca gataaaactg
    36841 ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt tcaacaggag
    36901 gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa gaagtgacag
    36961 ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc catggtggtc
    37021 ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa aatatccaac
    37081 caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg aaaccagatg
    37141 tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg ctccgtagtg
    37201 cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat gaagaagtac
    37261 tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa ctagcggcaa
    37321 aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta acggattggg
    37381 tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa acgcttcgtt
    37441 acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg gaaaaatatt
    37501 cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat atccgagatg
    37561 cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc gccttgaaac
    37621 acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg gaagcagcgt
    37681 atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga gaagtagatc
    37741 tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc ccttcttata
    37801 cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg acagtagact
    37861 ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt ggacttcttg
    37921 tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg acaaaacgta
    37981 agccgactcc agcagaagca aaagcattag tgtttgcttg gagagctgtc aaacatgtga
    38041 aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga gccgggcaga
    38101 tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa atggaaggtg
    38161 cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat gcagccaaac
    38221 atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat tctattgata
    38281 tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt agacattaga
    38341 gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa catgcaataa
    38401 gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca ggaaatccgg
    38461 ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat tcagcgttaa
    38521 tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa gttcctttgt
    38581 taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt ccgacaaaag
    38641 cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat acttatcaaa
    38701 tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg tatatcaaag
    38761 aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa ggtgtagtcg
    38821 tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac cagcatcgat
    38881 ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa gaattctccc
    38941 ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa gatcataagc
    39001 gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat tcaccagtac
    39061 cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa ccagcggctt
    39121 tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt ttgattgcta
    39181 cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct gaaacgcaag
    39241 tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta ttagagaatc
    39301 gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg gttgcagctg
    39361 ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg gaagaagagc
    39421 tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga gcaggaggac
    39481 gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa aaaatttaca
    39541 ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt ggagaaaaag
    39601 ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa aaaatacaac
    39661 aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt ggtacacttt
    39721 tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg tacttctgat
    39781 cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga agatccagta
    39841 aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc agatatttat
    39901 ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc aatcaaacga
    39961 gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt tgatttttct
    40021 aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat ggattatgtg
    40081 gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt agctgaagcg
    40141 tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa tcaaaaacca
    40201 atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa tcagctgcaa
    40261 tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat gacagatgaa
    40321 gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga tatgacgatc
    40381 caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg gaacgaaaaa
    40441 ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac gacaccaact
    40501 ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat cgccggtacg
    40561 atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat gtctcgggaa
    40621 gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt gtcataagca
    40681 ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt cagcttcgag
    40741 atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt tcgtttatta
    40801 gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa tcccttgttt
    40861 tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa aagcaatatc
    40921 tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc tttgttctgt
    40981 cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga aatgagaggg
    41041 acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg taaaattttc
    41101 tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg aaggctgtga
    41161 gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa gcgagtcgtc
    41221 ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt gcgggatttt
    41281 cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac gatataatcg
    41341 tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag atacttcgtt
    41401 tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat caactggaag
    41461 tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg aactggcggt
    41521 ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc tttctgcgac
    41581 aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat ggaccaacgt
    41641 ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa ctattaaaaa
    41701 tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc taaataaaaa
    41761 cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt ggtttgctgc
    41821 taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag caagtttcaa
    41881 tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat tttttttcaa
    41941 ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta aagcagttac
    42001 tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca gataatcatc
    42061 tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac taataaatat
    42121 gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc cgtccatgaa
    42181 aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg aagacagtgc
    42241 catctcacca tctccagctt cgccgacata agtgattgga taataagaac caaggacagt
    42301 tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa aaactttata
    42361 catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg acagttgttc
    42421 cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa taaagaagga
    42481 gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg atccctttgg
    42541 ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg ttgtctataa
    42601 ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat cctttgtgac
    42661 ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag atcattttta
    42721 aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc atatggccgt
    42781 atcttatttt taagatgact agataatcat tgatatggct aatttctttt tcaagaggaa
    42841 tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca tgggtcatag
    42901 tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc aaactgttat
    42961 aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt gctaaatggt
    43021 attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt ttattcatgc
    43081 tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag ccaaggttcc
    43141 ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg acctttctct
    43201 ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact ccaagttcca
    43261 ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt gctaagtcga
    43321 tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca gaatctacct
    43381 gattgatgat ctcttcattg gtatccataa aatcttttgc caagatatta ttgatcagct
    43441 cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac gtactaattt
    43501 ctttaagtgt atttgtatta tttttgctct ctcgaatatc attcctaagt tcttctagga
    43561 tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc cacatacttt
    43621 ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata tttgcgatcg
    43681 tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc aaagggataa
    43741 cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg cttaaatttt
    43801 ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat attctgctgg
    43861 gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt ttgaatattt
    43921 ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt acactcgact
    43981 acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc atcaatagtt
    44041 acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc taaagcaccg
    44101 tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc atttgtttct
    44161 aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac aattcggtga
    44221 acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt tggtttacga
    44281 ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt ctctgaacca
    44341 gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt gctgacaaat
    44401 aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac gaaaagctcg
    44461 ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc tcgctttgct
    44521 tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat catatccact
    44581 ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat gtagccattt
    44641 cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc agtatgcttt
    44701 ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg gtcaggtgct
    44761 tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag ggctaaaaaa
    44821 aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta tcttatcaga
    44881 taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa acttaaaaaa
    44941 atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa aaaatcaatg
    45001 ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga taaaaaatga
    45061 aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc attctattaa
    45121 gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga atcgctatgc
    45181 cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg gaattagaaa
    45241 aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat caagtagcac
    45301 aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct atcgatggtt
    45361 cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg atcgcctatg
    45421 atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat aattttggag
    45481 taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa ggaaaaggac
    45541 cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta atcaattaca
    45601 acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc gtgccatctg
    45661 gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg gcgcaagcaa
    45721 ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct gttttgtcgc
    45781 catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg tatgggagtg
    45841 aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc gttaaatcga
    45901 tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta gcaaaaaata
    45961 cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat gatactgaaa
    46021 cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta tctgtcgata
    46081 aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc gatctgaaaa
    46141 attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga taagaattat
    46201 ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa tccgagcgtt
    46261 agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg gagaaaacgg
    46321 agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg gctcctatga
    46381 cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag atagtgaaga
    46441 aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt caataaaaga
    46501 gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg aactgacaga
    46561 aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga atcttctagt
    46621 atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat tatcaaaatc
    46681 cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag aaagcgcgaa
    46741 tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca ttatttcaca
    46801 taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg atggacagac
    46861 gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg gaatggtggg
    46921 cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag tttattttaa
    46981 agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa acgaccacat
    47041 caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg gagcgggcag
    47101 aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat ccggacgtat
    47161 attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg caaacggact
    47221 cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg atattcggga
    47281 aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag ataaagaaaa
    47341 agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa actccatcca
    47401 tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat ccaagtggct
    47461 gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag atgttggggc
    47521 aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag ctgtctgtct
    47581 tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata cgatgaacga
    47641 aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct tgatgaaatt
    47701 aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg gataaaaaag
    47761 agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac agcatggtga
    47821 tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt ttctggcgtc
    47881 cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta gctgcaggga
    47941 tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg atggcgtttg
    48001 ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg atgacagtta
    48061 tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg gtagcttatg
    48121 cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt ggactgacac
    48181 aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa aaaatgtcta
    48241 cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca ttgattttag
    48301 gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat cgtcggaaaa
    48361 atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta tttacagcag
    48421 tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg attattttga
    48481 tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca gtagcaggtc
    48541 gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg atcaaaacaa
    48601 agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta gctggattag
    48661 ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg gaactggatg
    48721 caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga acaatcatgg
    48781 gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg attttaggcg
    48841 tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc gttgtgctag
    48901 acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca gaaaaaagac
    48961 tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca ttgtttcgtt
    49021 tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc tttttgctct
    49081 tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc ttgattaaat
    49141 taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat gaaagtattt
    49201 gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg ttctgaatgt
    49261 gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc tgccaacatg
    49321 cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta tttctatatc
    49381 atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa aaagcgtgga
    49441 ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat agagaagttg
    49501 gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg acatgctaac
    49561 tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt tgtgattgcc
    49621 ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc agatgcaaca
    49681 aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg ctttgggact
    49741 ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa gccgatcatc
    49801 gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt tggtgcgaca
    49861 atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga aacaaaagtc
    49921 gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca aaaaggcgaa
    49981 aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt gaaagataca
    50041 ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg aagagatctg
    50101 gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa tggagacaca
    50161 atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca ttaagtaata
    50221 agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt attatcgcag
    50281 atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt tttgtcacag
    50341 tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta aaatcaaaag
    50401 aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa aaacaaggac
    50461 cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga aggatcaatc
    50521 ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg aatcgttcca
    50581 ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc aaacgtggca
    50641 gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga atcgattggc
    50701 ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa ataagagaag
    50761 caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg ccagatgagc
    50821 ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg gaagaaaaag
    50881 aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat ttggaaagta
    50941 aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt cagcaagcaa
    51001 tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta gagtcacctt
    51061 ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt gggataccga
    51121 tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa aataaattga
    51181 aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg agtcaaaaac
    51241 gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt gaagacgatg
    51301 tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga acgattgatc
    51361 cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca acgaaaatcg
    51421 gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa gaaatgatgg
    51481 agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat gaagcaattg
    51541 atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg ataagcgctt
    51601 ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac tatgcctgga
    51661 ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta gatatgggtc
    51721 ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga atcaattaca
    51781 gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt ctagaaaagt
    51841 ggttaacaga ataatttcac aaagtaaata gattaaagaa tataacagct tggatttaaa
    51901 tttcaagctg tttctttttt ttcggaaaaa gaatacgata taaagaaact aagaaaaaac
    51961 cattatttgt acgtccgttc acgatgtgta aataaaataa atgtttgcaa caaaactgta
    52021 taacaccaac gtttttaaat agaaacagat ggttgtaagt ttgattagaa aatagaaaaa
    52081 atgcagatga aatataaaac gctttcctta ttgtgatttt attcactaat gctgttaata
    52141 tgcggattgg cataatttcc tttcatcgtg aaaatttgtg cttttagctt gtatttctca
    52201 taaatccatg ataatttatt aatgaagacg gttaaatagt gatacagttt tattcactgt
    52261 ttcaatcgca gaatgaagag attttcttat agatgagaga ggaatgtgtg acaaatggca
    52321 aacaaaaaag caccgatcga cttccaagca ctgctagatg aagtcaatgc agatttccca
    52381 gtatatcaag cacttgatca agatggaaac gtagttaaca aagatttagt acctgattta
    52441 tccgatgatg aactcgttga attaatgact catatggtat ggtctcgtgt attagaccaa
    52501 cgttcaacag cgctgaaccg ccaaggacgc ctaggctttt ttgcaccaac tgctggacaa
    52561 gaggcaagtc agctggctag tgcttacgca tttgataaag aagatgttct tcttccaggt
    52621 tatcgggatg tacctcaact agtaaaacat ggtttacctt tatctcaagc ttttttatgg
    52681 tctcgaggac atgctgcagg taattcttat ccggaagaat tgaaagctct accaccacaa
    52741 attatcatcg gtgcacaata tgtacaagca gccggtgttg cattaggttt gaagaaacga
    52801 aacaagaaaa atgtcgtctt cacttatact ggtgatggcg gttcatcaca aggtgatttt
    52861 tacgaagcaa tcaatttcgc tggcgcttac catgcaaatg ccgtatttta tatccaaaac
    52921 aacgggtatg cgatctctac acctcgtgaa aaacaaactg cagcaaaaac acttgcacaa
    52981 aaagctgtcg cagctgggat tcctggtatt caagtcgatg gaatggatcc gcttgcagta
    53041 tatactgtat ctaaaatggc acgggaatgg gcgatcagtg gaaatggacc agtcctaatc
    53101 gaaacactga cttatcgtta tggaccacat acactatctg gtgacgatcc aacccgctac
    53161 cgtacaaaag acacagatga cgattggcag aaaaaagatc cattgatccg cttccgtaaa
    53221 tatttgacgg aaaaaggact ttggtctgaa gaaaaagaag aacaagtcat cgaagcaact
    53281 aaagaagaaa tcaaagcagc tattgctgaa gccgacaaag taccaaaaca aaaggtttca
    53341 gatttcttga aaaacatgtt cgaagtatct ccacaatcaa tcaaagaaca aattgcaatt
    53401 tatgaagcga aggagtcgaa ataatcatgg cacaaaaaac aatgatccaa gcaatcacag
    53461 atgccttagc acttgaacta gaaaatgacg agaatgtcgt tgtttttggt gaagatgttg
    53521 ggaaaaacgg aggagttttc cgggcaactg aaggattgca ggaaaagttc ggagaagacc
    53581 gagtattcga tactccttta gctgaatctg gaatcgcagg attgagtttc ggtcttgcgt
    53641 tagaaggatt tcgtccagtt cctgaaatcc aattctttgg ttttattttc gaagcaatgg
    53701 acgaagtagt agctcaaatg gctcgtacaa gatatcgaat gagcggcact agaaatctac
    53761 ccatcacaat tcgttcgcca tttggtggcg gtgttcatac accagaattg cattcggata
    53821 atttagaagg tttgatcgct cagtctcctg gtattcgagt agtgattcct tccaaccctt
    53881 atgatgcaaa aggactattg atttcttcta ttagaagtaa cgatccggtt gttttcctag
    53941 aacacatgaa actatatcgc tcattccgag aagaagtgcc ggatgaagct tacgaagttc
    54001 ctttggataa agcagcagtt actcgtgagg gaacagatgt atctattatc acatacggag
    54061 caatggtgcg tgaagcaatc aaagcagcag acaatcttgc aaaagaaaat atatctgttg
    54121 aaatcattga tcttcgtaca gttgcgccat tagatgtaga aacgatcatt caatccgtgg
    54181 aaaaaactgg tcgcgtagtt gttgtccaag aagcacaacg acaagcgggc gttgccgcgc
    54241 aagtagtttc tgagatctca gaacgcgcta tcctttcatt agaagcccca atcggacgag
    54301 tttctgcacc tgatacagtc ttcccatttg gacaagcaga aaatgtctgg ttgccaaatg
    54361 caaaagatat cgaagacaaa gtaaaagaaa tcgcggaatt ttaagaaaag aagggacgaa
    54421 acaaagtcat ggcttatcaa tttaaattgc cggatatcgg tgaaggaatc gcagaaggtg
    54481 aaatcgtcaa atggttcgtc aaaccaggag atacgatcaa tgaagatgat acattactag
    54541 aagttcaaaa tgacaaatca gtagaagaaa tcccgtcacc tgttacagga acggttaaaa
    54601 atgtgattgt tccagaaggt accgtagcaa atgttgggga tgtattagta gaaatcgatg
    54661 caccaggaca cgaagacaat gaaggggaca gtggtgtagc cgcagaatcc caaactccag
    54721 ctaaaccggc tgctgaaccc actgttgata cagaatcagc tggatcgtca agcgaaggag
    54781 tcttccaatt taagttaccg gatatcggtg aaggaatcgc agaaggtgaa atcgtcaaat
    54841 ggttcgtcaa accaggagat acgatcaatg aagatgatac attactagaa gttcaaaatg
    54901 acaaatcagt agaagaaatt ccgtcacctg tcacagggac agttaaaaac gtgattgttc
    54961 cagaaggcac tgtagcaaat gttggggatg tattagtaga aatcgatgct cctggacaca
    55021 atagtgcacc gtcaactagt gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat
    55081 caggttcagc aagtgtcgtt gaagctgccg atccaaataa acgtgtattg gctatgccat
    55141 ctgttcgtca atttgcccgt gaaaaagatg tagatatctc acaagtaact gcaactggaa
    55201 aaggtggcag agtaacaaaa gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg
    55261 caccagcgaa atcagaagcg ccagaagcgg cagcaccgaa agaagcagct cctgcagcag
    55321 aaagcaaacc agctgcacca gcaaaaccat tcaagtccaa cttaggagac ttggaagaac
    55381 gtgtagcaat gacgccaaca cgtaaagcta tcgctaaagc aatggtcaac agtaagcata
    55441 cagctccaca cgtgacattg catgatgaag tagaagtgtc taaactatgg gataatcgga
    55501 aacgatttaa agaagttgct gctgcaaatg gtacgaaact tactttcttg ccatatgtcg
    55561 tgaaagcttt aacagcgact gttaagaaat acccagtatt gaatgcatct atcgacgatg
    55621 caaaccaaga aatcgtttat aaacattact acaatatcgg tattgcaact gatacggatc
    55681 acggactata tgtaccaaat gtgaaagatg cagaccgcaa aggaatgttt gctattgcag
    55741 acgaaatcaa tgagaaagca aaattagctc atgacggcaa actatctgca gaagatatgc
    55801 gcaatggaac aattacgatc agcaatatcg gatctgtagg tggcggttgg tttacaccag
    55861 tcatcaacta tccagaagta gctattttag gtgtaggaac gattgcacaa caaccaatcg
    55921 taaatgctga aggcgaaatc gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc
    55981 accgtatcgt agatggagca actgcgcaac aagcaatgaa taatatcaaa cgtcttttag
    56041 ctgatccaga gctattaatg atggaaggat gataaacaaa tggtagttgg agattttgct
    56101 gtagaattag atacagttgt aattggtgcg ggacctggtg gctatgtagc tgccatccgc
    56161 gctgctgaaa tgggacaaaa agtagcaatc attgaaagag agtatattgg cggtgtttgt
    56221 ttgaatgtcg gctgtattcc ttcaaaagca ctgatagctg caggacacca ttatcaagag
    56281 tcactagatt ctgaaatgtt tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa
    56341 acacaagaat ggaaagaaaa caaagttgtc catacgttga cttctggtgt tggtttccta
    56401 ttaaaaaaac ataaggttga aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca
    56461 ctacgcgtca ttcatccaga ttcagctcaa acttattcgt tcaataacgc aatcatcgca
    56521 acaggttccc gtccaatcga gattccagga tttaaattcg gaggacgtgt gttagattct
    56581 actggcggat tagcattgaa agaagtacct aagaaatttg tgatcatcgg tggcggtgtg
    56641 attggtgccg aattgggcgg agcgtacgca aacttaggag cagaagtaac gattcttgaa
    56701 ggctcaccac aaattttacc aacttacgaa aaagatctag taaaattagt agaagatgat
    56761 ttcaagaaaa aaggcgtaac agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac
    56821 ggtgacagcg tgacagtgaa atatgcagtg gacgggaaag aagaatccgt tactgctgat
    56881 tatgtgatgg tcactgtagg tcgtcgtccg aatacagatg atatgggctt agaacaagcg
    56941 ggtgtcgaag taggagaacg aggattgatc acggtggata aacaaggacg tacgaacgta
    57001 cctaacatct atgccatcgg tgatatcgtt ccaggggcgg cattagcaca caaagccagt
    57061 tatgaagcaa aaatcgctgc tgaagcaatc tctggcaaaa aagtagctgt agattataaa
    57121 gcaatgccgg cagtagcctt cactgatcct gaattggctt ctgtaggtat gacgatcaaa
    57181 gaagcaaaag atgctggtat cgaagcaaaa gcatataaat tcccattttc agggaacggt
    57241 cgggcattat cgcttggtaa aacagaagga ttcatccgtc tagttacgac gatcgaagat
    57301 aacgtgctta ttggcgcgca aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta
    57361 gctttagcaa tcgaatcagg aatgaatgct gaagatatcg ccttgacgat tcatcctcat
    57421 ccatctcttg gcgaaatcgt catggatgct tcggaattag cattaggttt accaatccat
    57481 atttgatttg aataatacag gcgaaacgca actgaaa
//
LOCUS       DACDUU010000008        54149 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.008, whole genome shotgun sequence.
ACCESSION   DACDUU010000008 DACDUU010000000
VERSION     DACDUU010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.008"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..954)
                     /locus_tag="IWL30_000525"
     CDS             complement(<1..954)
                     /locus_tag="IWL30_000525"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HAP9705316.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAE"
     gene            complement(1231..3033)
                     /locus_tag="IWL30_000526"
     CDS             complement(1231..3033)
                     /locus_tag="IWL30_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9705317.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(3047..3799)
                     /locus_tag="IWL30_000527"
     CDS             complement(3047..3799)
                     /locus_tag="IWL30_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9705318.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(3808..4170)
                     /locus_tag="IWL30_000528"
     CDS             complement(3808..4170)
                     /locus_tag="IWL30_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9705319.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(4346..5218)
                     /locus_tag="IWL30_000529"
     CDS             complement(4346..5218)
                     /locus_tag="IWL30_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAP9705320.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(5269..5757)
                     /locus_tag="IWL30_000530"
     CDS             complement(5269..5757)
                     /locus_tag="IWL30_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP9705321.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(5895..6725)
                     /locus_tag="IWL30_000531"
     CDS             complement(5895..6725)
                     /locus_tag="IWL30_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAP9705322.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            complement(6741..7349)
                     /locus_tag="IWL30_000532"
     CDS             complement(6741..7349)
                     /locus_tag="IWL30_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAP9705323.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(7342..7659)
                     /locus_tag="IWL30_000533"
     CDS             complement(7342..7659)
                     /locus_tag="IWL30_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9705324.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(7815..8543)
                     /locus_tag="IWL30_000534"
     CDS             complement(7815..8543)
                     /locus_tag="IWL30_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAP9705325.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            8702..9583
                     /locus_tag="IWL30_000535"
     CDS             8702..9583
                     /locus_tag="IWL30_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9705326.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(9820..10863)
                     /locus_tag="IWL30_000536"
     CDS             complement(9820..10863)
                     /locus_tag="IWL30_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9705327.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(10866..11534)
                     /locus_tag="IWL30_000537"
     CDS             complement(10866..11534)
                     /locus_tag="IWL30_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9705328.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            11676..14012
                     /locus_tag="IWL30_000538"
     CDS             11676..14012
                     /locus_tag="IWL30_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9705329.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(14127..14438)
                     /locus_tag="IWL30_000539"
     CDS             complement(14127..14438)
                     /locus_tag="IWL30_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705330.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            14619..16094
                     /locus_tag="IWL30_000540"
     CDS             14619..16094
                     /locus_tag="IWL30_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAP9705331.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPTMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            16141..16725
                     /locus_tag="IWL30_000541"
     CDS             16141..16725
                     /locus_tag="IWL30_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAP9705332.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            16727..17146
                     /locus_tag="IWL30_000542"
     CDS             16727..17146
                     /locus_tag="IWL30_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAP9705333.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(17427..17711)
                     /locus_tag="IWL30_000543"
     CDS             complement(17427..17711)
                     /locus_tag="IWL30_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAP9705334.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(18063..18773)
                     /locus_tag="IWL30_000544"
     CDS             complement(18063..18773)
                     /locus_tag="IWL30_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAP9705335.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(18770..20242)
                     /locus_tag="IWL30_000545"
     CDS             complement(18770..20242)
                     /locus_tag="IWL30_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9705336.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            20380..20853
                     /locus_tag="IWL30_000546"
     CDS             20380..20853
                     /locus_tag="IWL30_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9705337.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(21006..21557)
                     /locus_tag="IWL30_000547"
     CDS             complement(21006..21557)
                     /locus_tag="IWL30_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9705338.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            21662..22192
                     /locus_tag="IWL30_000548"
     CDS             21662..22192
                     /locus_tag="IWL30_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAP9705339.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(22675..23118)
                     /locus_tag="IWL30_000549"
     CDS             complement(22675..23118)
                     /locus_tag="IWL30_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9705340.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(23134..24294)
                     /locus_tag="IWL30_000550"
     CDS             complement(23134..24294)
                     /locus_tag="IWL30_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9705341.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            24801..26501
                     /locus_tag="IWL30_000551"
     CDS             24801..26501
                     /locus_tag="IWL30_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705342.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(26603..27436)
                     /locus_tag="IWL30_000552"
     CDS             complement(26603..27436)
                     /locus_tag="IWL30_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP9705343.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(27740..29905)
                     /locus_tag="IWL30_000553"
     CDS             complement(27740..29905)
                     /locus_tag="IWL30_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9705344.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            30165..32459
                     /locus_tag="IWL30_000554"
     CDS             30165..32459
                     /locus_tag="IWL30_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP9705345.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            32452..33117
                     /gene="pgmB"
                     /locus_tag="IWL30_000555"
     CDS             32452..33117
                     /gene="pgmB"
                     /locus_tag="IWL30_000555"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP9705346.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            33110..34117
                     /locus_tag="IWL30_000556"
     CDS             33110..34117
                     /locus_tag="IWL30_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP9705347.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            34139..35158
                     /locus_tag="IWL30_000557"
     CDS             34139..35158
                     /locus_tag="IWL30_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9705348.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(35439..36806)
                     /gene="nhaC"
                     /locus_tag="IWL30_000558"
     CDS             complement(35439..36806)
                     /gene="nhaC"
                     /locus_tag="IWL30_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP9705349.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(36915..38336)
                     /locus_tag="IWL30_000559"
     CDS             complement(36915..38336)
                     /locus_tag="IWL30_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9705350.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(38579..40456)
                     /gene="tdc"
                     /locus_tag="IWL30_000560"
     CDS             complement(38579..40456)
                     /gene="tdc"
                     /locus_tag="IWL30_000560"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP9705351.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(40747..42003)
                     /locus_tag="IWL30_000561"
     CDS             complement(40747..42003)
                     /locus_tag="IWL30_000561"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAP9705352.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            42583..43884
                     /locus_tag="IWL30_000562"
     CDS             42583..43884
                     /locus_tag="IWL30_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP9705353.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(43953..44552)
                     /locus_tag="IWL30_000563"
     CDS             complement(43953..44552)
                     /locus_tag="IWL30_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9705354.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            44806..45687
                     /locus_tag="IWL30_000564"
     CDS             44806..45687
                     /locus_tag="IWL30_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9705355.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            45700..47310
                     /locus_tag="IWL30_000565"
     CDS             45700..47310
                     /locus_tag="IWL30_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAP9705356.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(47387..48046)
                     /locus_tag="IWL30_000566"
     CDS             complement(47387..48046)
                     /locus_tag="IWL30_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP9705357.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(48329..48994)
                     /locus_tag="IWL30_000567"
     CDS             complement(48329..48994)
                     /locus_tag="IWL30_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705358.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(49141..49746)
                     /gene="dhaL"
                     /locus_tag="IWL30_000568"
     CDS             complement(49141..49746)
                     /gene="dhaL"
                     /locus_tag="IWL30_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAP9705359.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(49774..50757)
                     /gene="dhaK"
                     /locus_tag="IWL30_000569"
     CDS             complement(49774..50757)
                     /gene="dhaK"
                     /locus_tag="IWL30_000569"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAP9705360.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(50759..51154)
                     /gene="dhaM"
                     /locus_tag="IWL30_000570"
     CDS             complement(50759..51154)
                     /gene="dhaM"
                     /locus_tag="IWL30_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAP9705361.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            51308..51628
                     /locus_tag="IWL30_000571"
     CDS             51308..51628
                     /locus_tag="IWL30_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAP9705362.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(51740..52609)
                     /locus_tag="IWL30_000572"
     CDS             complement(51740..52609)
                     /locus_tag="IWL30_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9705363.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(52705..54009)
                     /locus_tag="IWL30_000573"
     CDS             complement(52705..54009)
                     /locus_tag="IWL30_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9705364.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
BASE COUNT        16158 a        11191 c         9688 g        17112 t
ORIGIN      
        1 ctccgctcct ggcagttctt gagtctcaaa tgtgataccg ccatgattta ctaatttatt
       61 cccgcgcatt tccggtccat cttctccaaa atttgctgta aaaatcacga ttcctgaagc
      121 atctgttttt acttggatcg atactttttc atctgggctt gtcaattccg cagcatttag
      181 taaaccagat tgttttttca acaaaaatgg atgatcgatg ccgcccacta tcattgattg
      241 gcgaaaatca ctggcaaaga cctcacctaa ttttttagga cgttggaagt caaatgctgt
      301 gccacttact gaaactactt ctcctgtagg cgttgtatct ttattgagcg tagcaaataa
      361 tcggctattc aaccaaagta tatgttcgtc gattgactgt gctggatcac cggttaaatt
      421 gaaatagaca tgatttgtag gattgaatac tgttttgcgg tcactgatcc cttggaccgt
      481 cactcgccat gtatcatcgt tcgtcagcgt ataccgtact tctacatcta aagttccagg
      541 aaatccgttt tccatatctg ggctagtctg atagaaaatg accgatgctt cgttttcacc
      601 attgatgact gtataatgcc actgcttggc ttcaaagcta ggtgcaccac catgtaaaca
      661 atgaccagtg tccgggtctg tttttacttc gaacgtttca tcatctaatt tgaatgtgcc
      721 attcgtaatc cgtccagcca tacgtccgat tgaagcaccg atataagggt ctttttctag
      781 atattctttt gcttcatcaa aacctaagat caactctcgt ttttcattat cgattattgt
      841 ttgaaaatga acaacccgtg ccccaagatc actgacagac aaagatgtcc cattttgatt
      901 cgtgatcgta atcaagtgat agttttcgcc aaaattttgt tcattgattt ccatgttcct
      961 tgcaccctct tttctttcat ttgcactgat tccagcatac atggaaaccg ttttcttaac
     1021 agcttattcc gtgagtaacg agaaaaaatt tactatattc taataaaaga ccgtgaaaaa
     1081 aaggttgtga caaaaatatt gtcacaacct ctttttccga acaaacggtg ttcaaaagct
     1141 tgcatctgcg gtaataagcc caaccaaacg aaaaatatga ggagctattt ttgcttggct
     1201 gttcttatta cttgatgcag gacagctttt tcacaatctc tttttcaata cgattccagt
     1261 atataaccat gtcgtcagta catagtagat gaaatacaat acgaagaata tcgtaaacgg
     1321 aacccaaata tctttgtatg gattttgcat gaatagacca aacatttgta atccgaataa
     1381 gacatggact acacctaaga ttcctggtgc taagaataac acgcctactt ctctacggat
     1441 cgattgttta agcaacccgc gtcgtgttcc tatcttttca agcatttcat aacgagcaat
     1501 gtcactcttg cttcctgaca agatcttgaa catcaaacag ctagcaagca tggtcaagaa
     1561 agctaaacct aggaagaatc ccataaattc aaaaccagag aatagaccat tatacacttc
     1621 atacatttca tatttctggt tgctgtactc tactttttgc aaagatggat tgttcatttt
     1681 attttgagca acaagtgttt cgattggttt caggtttgct tggaagtctt ttacctgaac
     1741 tagctgtaaa gtggattgtg gcaagttcag ttgatcaaat tctgattgac tgactacctt
     1801 gatttctttt tcttgctgtt ccggcaattc atagcttcga agttcctctt gtacagtcag
     1861 ttcattcatt tttttagcag gaactttttc tttcgtgatc ttcggcggtg ttccatcttc
     1921 atgttttatc atgatcaatg gttgagccgc aaattcactt gcattatagt aaatcgttcc
     1981 cgcatcttct ttttgagtat aagaagcaga aagtgttggt gaaagttctt tgattttttg
     2041 ctgatcttct gcccgaggat tattcatcac tagatcatat ccagaattgt tttctgtcaa
     2101 gtctgcaatt tcatttttaa aacctaatcc taccgtcaaa gcgcccagtg ccaaggcaaa
     2161 cagcatagcg accattgaga gcatttgtgt atattcacga atacgaaagt ttaactgtga
     2221 caatgtgaaa ttgttcaatt ttttcaatga aatctgttct gatttcttta gtaatgataa
     2281 aaagaaaatg atcacagaat ggaaaatgaa ataagttcct aatacgatcg taactaaggc
     2341 tacccctata ccaaagactt gataggtccc cacgtgtttc atcatatagt aaccgcagcc
     2401 tagaaaagca attcccagta ctacttcaag gaaaaacaag aatttatgct gcttgattct
     2461 tgttggtgtc tgatccgcac gaatcaatgc taaaatcggt tttttgacaa tcgaaaaagc
     2521 attgacaaca gcagctaata aaaacaaaac agcaaaaaac aaaacagtga tcaacaaccc
     2581 gtgaatacta aaaggtgtaa aatgcgaaat attgagatct aactgatcca ctaataatcg
     2641 gtggacgata cttgtcaatc ctacaccgac agctgaaccc actaaagtag ctgcgatccc
     2701 tactaagaat gtttcagaaa aaatcatctg tgcgattttt ctgccttttg ctcccagcat
     2761 cataaacatt gcatagtctt tttgccgcat cgtcatcaaa aaggaatttg catataaaat
     2821 ataaacgaat gtgatgatcc ctagtaaaac agaaccgaat cggaaaatga taactgtagc
     2881 cgcaacgatc gtattacttt tcaaaaaagc ttcgttgctt gccattgatt caaacatgta
     2941 aaaaatagca gatgccataa ctaatcctga gaataacacc aagtagtctc ttaaacggct
     3001 cttgatccca gtaagtgata atttccaaag catagtcaat ctccccctac tgttccaagt
     3061 ttccaagaat agatagtatt tctcgataga aggaatcacg gctttgatct ccacgtttca
     3121 cttcttgatg gataccgcca tccttgatga ataaaatacg ttggcaataa cttgcagaaa
     3181 acggatcatg cgtcacaaga agaatcgata catgatcttt tgtattcaat tcgtccatcg
     3241 tatctaaaag atcccgggca cttttggaat ccagcgcacc tgtaggctca tcccctaata
     3301 gaatcgttgg ttgtgtgatc aatgcacgtg cggcggcgac ccgttgtttt tgtccaccgg
     3361 aaatctcaga aggatagctt tccaaaatat gagagatgga taaacgttct gcaatttttt
     3421 ggacacgttg cttgatttct tttggtctga cgccttgtaa agaaagagga acagcgatat
     3481 tttcttgtgc tgtcaagctt tctaataaat tgaaatcttg gaaaataaag ccaatctcat
     3541 tcgcacgaaa atctgctagt tgatttcctt ttaatgtggt cacatctttt tgattgattc
     3601 ggatatgccc atctgttggt ttatctaatg tggataaaat attcaaaagt gtcgacttac
     3661 cagaaccaga agcgcccatg atcccaataa attcgccttt ttctacatcg aagctgatgc
     3721 cgcttaatgc ttgcgtcttt ttctcattgt tttttccgta tacttttgtt acattctgta
     3781 cttcgactat tttgttcatt tctttcccta ccctttaatt agtttttcca gtgctgtttt
     3841 cggcacgtct tttttttcga cttcttcata cgaagtgaca cctttatttt tgttccaggt
     3901 gatttttaaa tatgctcctt tacgaagcgg tcgagtcaat tgtcccttga agtccagttc
     3961 tttttcctta ccctctttcg tatagccagg caggatatat ctatagttga ctgtttcttg
     4021 tccagagtct gattttgaaa ctgtttttgt accatctgtc gtaatttgta catagtattc
     4081 ttctccaccc ataactacgt aatctgcgat tttcacccct gcaagtacca caacgatgaa
     4141 aacaagtaga ccgatcagaa gttttttcac tttttcccct tctctcttac tttatatact
     4201 gattatagaa aaaagcgaac aattctgcct tcgaatcaac tatcattctt ctttcaattt
     4261 tgtcattttc tttaaaacaa ctaaaaaaag agttcaagac aataatccgt cctaaactcc
     4321 ttcttcctat cttacaaaaa gtatgtcact tacggtcttt catgtcgcct gtattgataa
     4381 caggttgcgt tcctttgtct gtaatgattg agacaagtaa attattgatc aattggactt
     4441 tccgatcatc ggtgaaattg atttcttgtc catcttcgat ttgctctaag gccatttgag
     4501 tgattgtgac agctccttca acaatggttt gtcgagcaga caaaatggct tttgcttgtt
     4561 gacgttggag catggcacta gcgatttctg ttgcataggc taaatggttc aagcgtgttt
     4621 cgattacttc gacacctgct actgctaaac gctcttgcag ctctttttgc aactcgtcag
     4681 acactgccgt cgtgtttcct cgaagcgtca aatcattctc attgaatgta tcatacgggt
     4741 attggctagc gatatgccga atggctgtct cactttggat ttctacaaaa tcttgataat
     4801 acgctacatc gaataatgcc tttgctgtat caacgacttt aaagactact actgctgaga
     4861 tttcgatggg gtttccatcc aaatcattga cttttaaaac agaactattg aaattccgaa
     4921 ctttcaacga aacagtcatt ttttgtgcca aaggtatcgt aaggaaaaag ccattttctc
     4981 gaatcgtacc taaatattgt ccaaaaaaca aaattacttt tgcttgatta ggactgacta
     5041 cagttgccga acttaacaac aataacgtga tcaaccaaag aaaaatactt aaaaaaagtg
     5101 cccaaacttt cgcttgccac atacctaaat agaagaaaaa caatccaact aaagctaata
     5161 cgaccaatcc gacaatccct gcataaccat tgatataaaa tgctcttttt tctctcatgc
     5221 tacttcctgc gctcctttat ccttcaataa atatctaatg ttactttttt aatttttttt
     5281 tcgaatacgc gccattacac cttctagttc taactctttg tcttcagtga tgtaccaacc
     5341 gcttgcctgt gtccctccgg ctgcctgcgg taaataatcc aattttgttc gaacccagtg
     5401 cttttcttct gtaaaatagt cttctaccaa gacctctaga tccatccctg ctgaaagagg
     5461 caaatcttcc cgaacagatt taaagataaa tcgttccttc ttcccatcat acttcaatat
     5521 tcctgtatcg ataatcggta aagaataata atccagctct ttcttcaccg tcaataaatg
     5581 tttgaaaact tcatggtaca cttgctctaa ttggcgacct tctgaattgt tcgggtaatt
     5641 aaccatcact ttttctttgt ctggatcata gaaggtttgg cttagttgag tgatataagt
     5701 atgtaatctt tccatttctg tttttaactt ttctctttga ccttcttcta ccttcatata
     5761 ctctttcacc ctgttttctt tttagatatt ttctttctta ttttactata gttatgtgcg
     5821 ttaagcgatg aaaaacagca aagattggaa ggatctttgc tgttttttct taaaatatcc
     5881 agttgagtac aggatcaatc tgtttcattg tcctcttgat ccgtcgtaat attgatcgat
     5941 gtgtctttag cagtgatctt tagctttttc tgtccttgtt ctaacaaagt atttgttatt
     6001 ggctggtagt ctttttcaat ataagattca ccagacgttt ggatagtata gccactgttt
     6061 tccatctcat gcaaagataa attgccaccg tttaggatga cttttccttc tttgctgatc
     6121 aaactgtctt ctagatggaa gttgctgttt tcacctgtga cgatcaagtt atccacattg
     6181 gcctcatcta atcgaagtga gcctaattca tctttcgcag ttattgttcc tcctgtaaat
     6241 tcattgaacg atacccagcc atcatgtgtc ttagtattca cttttttgat tgatacttcg
     6301 ttgatatcaa ttgcactatt attcccttcg attgtcaatt cagataattc atgatctgca
     6361 gggacaacaa tagtgatttg tgcacttgtg tcttctaaac taatgattgc acctgttgat
     6421 tttttgactg cattcacagt caacacgcca tttttctcct ttatttctgg cttttctgtc
     6481 ttatcataag tcgtttttat ctcaaaatcc aatccgcgta tgaccgaaac tgtttgattt
     6541 tctgcatgga tgacgatttt gtttgcatcc ttgatttttt ttgtctcagt aaccatatcg
     6601 atcaaccgag gaccattatc ccaagtcaga ctcttttgag cgcctaatgt ataagttgca
     6661 cctgctagga ggccaccagt aagcatcaga ccaataccgg taatcaaaaa atgtttcaat
     6721 ctcaatttat gtgtcctcct tcaaacgccc gctgaatgca gatctctttt tgtaataaaa
     6781 cgtcgtccca tccatttaac aaagttcatc atgacataaa aaagcccttt tgttcctttc
     6841 agcaacagtg gcgaaataaa tactgcgctt cctgtggcga acagaccgac accgataaag
     6901 aataatccac tcagaatcga ttgtccaaac acactgattc ctgagatgat catgaatagg
     6961 ccaacaccta agatactgat acataaaaca tagatactaa aaatgagcgc tagtaaagca
     7021 accaatagtc ccccaataac caatataaac cccagtgcta gtggaatgag gacaggagaa
     7081 gcaaataaac cgataatgat taaccaaatc aggcgaattt gtctttttgg atttttcact
     7141 aactcgtttt ctgcttgttc ttcttctgaa aagtaactga tttttaattt taaggcaaac
     7201 tgctttggtg taccatattc atcaatgatc tgatctaatg tttttccaga atctaaaaaa
     7261 tattcctcat aatactggat caaatcaaaa cgatcgtctt caggaatttc ttgaagatga
     7321 tccacaactt cattcaaata ttcattcatt tgtgctctcc tccaataacc ggtctaaaga
     7381 atctcgaaaa acaatccatt catcaataat ttcttctaaa tgacgacttc ccattggtgt
     7441 caacgagtaa tatcgacggt ttctgccttc aaaaggttca tcataggtct caagatatcc
     7501 ttcttttttc aatcttctca aaaccggata tagtgtagat tcactgacag agagactttc
     7561 ttgtacgatt tttgttaagg catacccata catatcttcc ttgcttaatg ctgctagcac
     7621 agtaccatca agaagaacag ttggaatctg gatgtgcatt acgtcacctt ctattctcaa
     7681 gtgtaatatt ttatttcaat ctatattata caacatataa tatagtttca aaatcagttt
     7741 gtcaaataca aaaaaactga ctaaaagatt tgatcctttt tagtcagcaa aaaatggctt
     7801 ctctttgatt tttattataa cagtggcata atccaatttg tccacatcca gctcagtggc
     7861 atgataacga tcattgcagg tatcatgtca gctactggga acattttcac gttgatcatt
     7921 ctaaaaccgg tagcgaccat caagaatccg ccgcaagctt taaaatctaa gatcatgtct
     7981 ggtgtagtca atggataaat aaattttgcc aataagaaca aaatataaaa aataatgaat
     8041 tggggaattg ctatcacaga aacgacatac cctaaattac acgcaaaaat agctgctgtg
     8101 aaaaaatcaa ggacagattt agaaatcaaa atcgtactgt ctcctgtcat cccagaatct
     8161 aaacttccat agatccccgt accacttgca caaaatagaa caatgaccgt cactaatgtg
     8221 ctgataaatt catcgtgggt cataccaagc ttttcactag ggaagatttt agaaataggt
     8281 acttgcatca gcgcagcccc cttatttatc ccattaccta aatgaacaat caaaccaatc
     8341 gctgttccta tcactaaagc aaaaataaca gccggcatat ttttcatcgg cgcaatggaa
     8401 tagattccca tccccatcga acacacacca aaaaccatcg tcagctgtgc tttaaaatgt
     8461 tcacttaatt tatttcctac taatccgcca attagtccgc caaacaaaac ggaacctaca
     8521 ttgatcaaaa ctccagttgg catcaaaaaa ctcctctctt tttctacgaa gcgaaaaaaa
     8581 tcagaaaaat ttctgtaatt attgctttcg ctgcaagaaa gattgtaaca gacagagaag
     8641 attacaccaa ttcatttttt gtgttaaagt attctttatt ggaataagta ggaggaatat
     8701 gatggatatc cataaattcg aagtttttct tgatttagcc gaaacaatga atttcaccaa
     8761 aactgctgat cgacaattca ccacccaagg aaatatttct aaacagattc tttcgttaga
     8821 aacagaatta gacgtcaaat tattcgaacg cgcccataga aaaatcgaat tgaccgaagc
     8881 tggaagctta ttgctcccat atgtaaaaaa tgtcgtagaa caataccatg ctatgcaaga
     8941 agtgcttgaa acctatacga aagacaacaa tctttcatta aatatcttaa cgataccgac
     9001 aatgatcaat tataaaggtt tttccaaaat caccgagttt ttgaaacgcc atcctgaatt
     9061 tacagtccag ttgaaagaag tggaaagtgt cgaattaact tacaatgacg ccaatgaaca
     9121 tagtgacacg atccactttg ctagaagttt ccaggcccca ccaagttcga ttgagtggtt
     9181 gcctactgaa acagacgatt ttgtagcagt tctacctaaa aatcatcctt tagctagtaa
     9241 aaagaaattg gatctaagcg aactgaaaaa ggagaacttt ttgctccttg gccccactac
     9301 gaacttgtat caacctgtac tagatctgtg tcgcgaagct ggatttgaac caaaaatctc
     9361 ttataaagga gcaagaatcg acttgatcat caatatgatt gcaaatgaca tggggattgc
     9421 tattgttatg gaaaaaacag tcaagaatct attaaaagaa aatactgtga ttctccctat
     9481 ttctccaaca acagagagtt atcttgcatt tactcgtaaa gtcggtgagc attctttggc
     9541 ttcagataca ttttgggagt atttaaatca agaagaaaac taagccctac attttctgtc
     9601 catctatcca cccaaaaaaa gagagcgacg catgactttc gctctctttc ttgcatcatg
     9661 tccttgtttc aatgacacct ctaacatttc tcgtagttca ataagaaagc agcgattttt
     9721 atgccaaaag aaccgtaaga acatttccct aatcacaaaa aaacgactaa aaacccctaa
     9781 aaaccttcaa tagaattttt aggggtttta ttctttttcc tattgtttac ttaaccgtat
     9841 cattgctgtc gttcctactc cttcgatact gctataatca atacgtccat catgcagttc
     9901 tagaattttt ttaaccagtg aaagtcctag cccattccca gaaatttgac gacttgtatc
     9961 tccttgataa aatttttcaa acattcgatc tctggtctct tcattcattc ctacacctga
    10021 atcggtcact tccagtacaa tctctgtggc tgtctcaaat agtttgatat gaatctgtcc
    10081 attgatttga ttgtacttta tggcgttatc catgatattc agccacactt ggtacaaaaa
    10141 ctcttcattg cctgtgtaat ttactgcagc aagttcgata tccaattcta gctgttcttt
    10201 ctcccattta ggctgaaaaa aaacgattac ttctctgatt tgttcatcta atcgatactc
    10261 tttcttttcc agttggatac gctggttttc cagtttgttc aattttaaga tgttctctgt
    10321 taattgtgaa agctgcgtga tactttcgat aatccgatat aaaaatattt ggcgttcttc
    10381 gtcagagaga tttggggctt gtaacaattg aacatatcct tggattgttg ctagtggtgt
    10441 tttgaattca tgggatacac tggacacaaa gtcattcctc agtgtctcga tactattgag
    10501 ttcttggacc ataacgttga aatctttata cagttgctgt acttctgcta ctttctgctg
    10561 ttcatccatt cgaatcgaaa aatctcccgt tgctaccaga ctcatatttg tccctagcgc
    10621 actgattgga tgcaggatac gccgcccaac aaaaatcgat attcctgtac ccacgaccat
    10681 actgaacccc gcaaagacga ataacgggta taacgttggt tttccagccg attcagcagg
    10741 cgagatagaa tgtcgagaca ataagaaaat aaaaccgaga aaacacagca gtgtcactaa
    10801 gatcgaaatg aagacaatac tagcaaaata gatccataat tgtgaaaata gctttttttt
    10861 catctttaaa taaccgcctt atagcctagt ccccgtactg tgatgatttg aaaatcttcc
    10921 gtcttttcaa aacgggtcct taaacgtttg atatgtacat cgatcgttcg ttcttctgta
    10981 ttcgtatcta atccccaaat atcatccatc aactgttggc gagtgaagat cttgttggga
    11041 taagacagta atttatacag aagcagaaat tctttttgag gcagaatcaa cgaatgctga
    11101 tcttgtaaca ctgtataagc atctagctct aattttgttt gtccgattat caagctactg
    11161 ctgtaattga tctttgaacg tcgaagcaat gcttccacac gtaggatcat ctcattgaca
    11221 tcgatgggtt ttgtcatata atcatctgct cctaatttga acccgcgttt cttatcctca
    11281 aaatcggcct ttgccgtaat cattagaaca ggtgtgtcaa atctggtgct acgtaatgag
    11341 tctagcaagt catagccatc cattttcggc atcatgatat ctgtaatgat taattctata
    11401 cgtgtcgctt ctagtttttt cagcgcatcc tctccattca atgcggtgat gactgaaaag
    11461 cccgctcgtt ccaacacaga acggtaaagc aactgtaatt gcggatcatc ttctactact
    11521 aaaatacgat tcattgatct ccctctgttt tttgaaaaat ttctagattt tttcttgtag
    11581 ttcatcttca gttaacattt atttcgtata ctacgatcat tgttctgata tacttattag
    11641 tataccgaat atttcaatac gggagaaggg tgattatgct tcaattaaaa gatataaaaa
    11701 aatattataa agtcggcgaa accaccacca aagcattgga tggtgtgtct gtcgctttca
    11761 gacaaaaaga attcgtagcg attttaggtc ctagtggttc tggtaaaacg actatgctga
    11821 atgtgattgg tggactagat aattatgatt ccggtgatat ggtaatcaac ggaaagtcta
    11881 ctaaagactt taaggacagt gactgggatg cctaccgaaa caactctatc ggtttcattt
    11941 ttcaaagtta caacttgatt ggtcatctag gcattattga aaacgttgaa cttgggatga
    12001 cattaagtgg tgtttctaaa gacgaaaaac gaaaacgggc agaagatgca ttgcaccgag
    12061 tcggcttgac aaatcatatg cataaaaagc caaatcagtt atctggtgga caaatgcagc
    12121 gagtggccat cgctcgagca ctagccaatg acccagatat ccttttgtgc gatgaaccaa
    12181 ccggagcgtt agatacagaa acaagtatcc agatcatgga attgatccaa gagttatcga
    12241 aagaaaaatt agtgatcatg gtcacacata atcctgaact ggcccatcaa tacgcagatc
    12301 gaatcattga attttctgat ggtaagatct taacagattc ccatcctcac atcgaacgac
    12361 caaaagatga tcaatttaac ttacgcagaa caaagatgag tttttggaca gcgttaaagc
    12421 tttccttcaa caatatccga acaaaaaaag gacgtacttt cttgacctcc tttgcttcca
    12481 gtattgggat catcggaatc gcgatcgttc tgtccctttc ttctggattc caaaaacaaa
    12541 tcgataacac gcaagctgaa acgatggcga aatttccgat tactatttct aaggtaacga
    12601 ctaaccagac tcgtgacgat gccggattgg gtgcaagcaa agcggattat ccagacagca
    12661 aaacgattac tgcaaaagtc agcgaagaag ataaagcaca gcacacgaat aagattgatc
    12721 aaacatacgt cgattacgta acagacatcg atcctaactt aagtaataat atcggattta
    12781 cccgtactac aggaatcaat cttttacgtg atgtaaatgg caaagtacag cctgtcagct
    12841 tctcgaatca aaatcctgat acggaatctc tttctttatc tagtacgatg tcagcaatga
    12901 caggtgtggg agtgtcaagt ttccctacac agcttgacac tagcaaagaa aactttttaa
    12961 aagacaatta ttctttactt gccggaagtt atccagcctc agcaacagat gtcgtgctga
    13021 tcgtagacgg caataataat actaatatca acgcattgaa gaatcttgga tttgatgtga
    13081 aagaagacga aaaacttgat tttgatgata tcgttggtac aacattcaaa ttagtaaaca
    13141 acaatactta ctatacaaaa cttccaactg ggaattttat ccctaataca gattatgatg
    13201 cgatgtatca gaatgcttct gatgaattga agatctcggg gatcctacgc gtgaaatctt
    13261 cttctacaat gaatctctta tctccaggaa tcgcttatag cgatcaatta acgactcaaa
    13321 tcgtcaacga aaacaaagaa tcagaaatcg tcaaagctca aagagacagt ggtgtaaatg
    13381 tcttaactac tgaaaaagtt gacgaaaatg ccaaacaaac attgcttagt tatttaggtg
    13441 gagacagctt gccttcaagt atcatgattt atccgaacaa ttttgaagac aaagaaaaga
    13501 tcttggatta tctagatgat tacaataaag gcaaatcaga tgaagataaa atcatttata
    13561 ctgatttagc tggaacgatg actgaactga ctggcggatt gatggatgct atcacttatg
    13621 tccttattgc ttttgcaggg atctctttag taacaagtat gatcatgatc agtatcatta
    13681 cttatacatc tgtgattgaa cggacaaaag aaatcggtgt actaaaagct ttaggtgcac
    13741 gtaaaaaaga catcactcgc gtatttgacg cagaaacgtg tattttaggt atttcttctg
    13801 ggatattagg tgtgtttatt gcatggctag ctacgttccc tatcaatagc atcttataca
    13861 acatgacgga tctaaaaaat gttgctcaac taaatcctgt ccatgcgatt attctagtga
    13921 tcgtctctac tatattgacg atgcttggcg gtcatcttcc tgcacgaatg gcagcgaaaa
    13981 aagacgccgc tatcgctttg cgtgcagaat aaggttgtga caaccacatt tagatttgag
    14041 tcataagagc gctgaaatag tagaaaacag caagaaacga ggaagtaata tgttccttgt
    14101 ttcgcgctgt tttttttaat tattttttat aattctggta tacttttctt tcctttgtac
    14161 cacaactctt ctggcgtata cctgtctttt ccatttatga atactgttcc atctgcttca
    14221 atcgttactt cagtcccaat ccacatatta cgagaaagtg cgagaagatt ttttcttttt
    14281 cctcttcctt tttttccaaa ttgcaaggca tcggttatct cttctgtcca atgagatttc
    14341 acgtatttta cttctccgtt ttcaaatgta actttcaaaa tattcgctat attttccact
    14401 tttactgctt taggaaatga ctcctccatt tttttcactt tccttcctcc aacactaagt
    14461 atgcgctttc ttttagtgat aagtatatca tagtttattc tattatcaaa aaaacgcatc
    14521 cattccattg tagaatactt taccgaacaa gttgaattgt tttcctcttt ttcacctttt
    14581 actatagata ggtaaagaca actaaggagg aatctctcat gacagatcaa ccgtatgatt
    14641 taagaaaagt catcgaagaa ctaaaaacac ttccaggtca atatcacgaa acagatattg
    14701 aaatcgatcc tgaagcagaa ttatctggtg tttaccgtta tatcggtgct ggcggtacag
    14761 taaaacgtcc gactcaagaa ggaccaacta tgactttcaa caatatcaaa ggtttccccg
    14821 gcacacgtgt gaacatcgga acaatggcta gtcgtaaacg tgtaggtgaa attttgcacc
    14881 atgattatcg aacacttggt cgtttcttga aagacgctgt agaaaatcca atcaaacctg
    14941 tgaaagtaga caaagcagac gctccagcac aagaagtcgt tcatctagct accgatgcag
    15001 acttcgatat tcgcaacatt ttgcctgcac ctacaaatac tcctgaagat gctggtccat
    15061 acatcacaat gggtgtagtc ttaggatcag atccagaaaa aacaatgagc gatgtaacga
    15121 tccatcgtat ggttctagaa gataaagata cgatcggtat gtacatcatg cctggcggac
    15181 gccacattgg tcacttccaa aaacaattcg aagccttgaa taaaccaatg cctatcacta
    15241 tcaatattgg tttagatcca gcaattgcca ttggcacgac tttcgaacca cctaccactc
    15301 cacttggcta caatgaacta tggtgtgcag gagctttgcg taacgaacct attcaaatcg
    15361 ttgatggtat agcagtcgat gaagtgggta ttgctcgtgc tgaattcatc attgaagcag
    15421 aaatcatgcc aaatgaaaca atgcaagaag atatcaacac gaactcagga aaagcaatgc
    15481 cagaatttcc tggctatact ggggatgcta atccagctgt acaagtagtg aaagtcaaag
    15541 cagttactca ccgaaaagac aatccaatca tgcaaacaac gattggtccc agtgaagaac
    15601 acgtatctat ggccggtatt cctacagaag caagtattct tgccttggtc gataaagcta
    15661 ttccaggaaa agtagtaaat gtctacaatc ctcctgcagg tggtggtaaa ttgatgacca
    15721 tcatgcaaat ccgtaaagaa aacgaagcgg atgaaggtat tcagcgtcaa gcagctattc
    15781 ttgctttatc agccttcaaa gaattgaaga cagtcatttt agtcgatgaa gatgttgata
    15841 ttttcgacat gaacgatgta gtttggactt tcaacactcg tttccaagga gataaagata
    15901 tcatcgtgat cccaggaatg aggaaccatc cattggaccc atcagaatta ccagaatata
    15961 atcctggctc tattcgtttc cgtgggataa gtgcaaaaac tatctttgac ggtacagttc
    16021 ctttcgatat gaaagaacat tttgtacgtg caaaattcca agaagtagaa gattgggaaa
    16081 aatatttgaa ataatgaatg atctgcaact gttttgacat cataaaaagg agtgcggaaa
    16141 atggaacaat tggcacaacg gaagaaaaaa atcgtgattg gtgtaagtgg cgcttcaggc
    16201 accatttatg ccatcaacct agtgaagaaa ctaaaagaat atccgatgat agaaacacac
    16261 ggaatcatca gtccatgggc aaaacagaac ttaaagctgg aatccgactt atcactaact
    16321 gaattcgaaa gcctgtttga ttaccattat tcaaacaaag atttaggtgc tgctgtcgcc
    16381 agcggatcat ttttgaccga tggaatgatc atcgtaccgg caagtatgaa aacagtcgca
    16441 ggtatctctg ttggtttagg cgacaacctg attgcacgtg ctgctgatgt aacaatgaag
    16501 gagcaacgaa agttagtcat tgttcctcgt gaaacgccat taaatgccat tcacttggaa
    16561 aacttgacta aattagcacg actaggtgtg caaatcatac caccaattcc tgctttttat
    16621 aatcagcctc aaacgatcca agatcttgtt aatcaccata caatgaaact tttggatcaa
    16681 tttgaaattg taaatgagca ttcaaaacgt tggcagggtg attaatatgg aaaaagaaac
    16741 gatagttgaa gtaaccaaac taggtttcac gatccaagct gctgttaaag taatcggtaa
    16801 agacttattg atcactttaa caggcggaga tacaccacat atcggaacag tcactacatt
    16861 ttctcgtgat acagagatgc aaactgtacg tttccctagc cacgatggtc ggttccataa
    16921 agacgatgtc ttagccgaaa aaatagctgt gatcatcaaa actgccgttc cgggaagttg
    16981 tacgatcact tccggtatcc atgttgacca tatctccaat gcacaaatca acgcctcttt
    17041 tccgatgtca gaagagttag gttacaagat tttagattgg ttggaaaaaa tagatttcca
    17101 tatcaaagaa ccagtttatt ataaagatgg tgaaaaacca ttatgaaaga agaggttgtg
    17161 aaatcagcgt tatgacctga gtatgagaga aaaaaatgga aaaatagctt ctcatatttt
    17221 gccgtttttg agctaatacc gagggacagc tgattgaacg ccgtttatca agaggttgtg
    17281 ataaagccgc ttcgtttcga gtagtaagaa aaattttgta aacatgggtt tccacatttt
    17341 tgcataaatt tgacttacta ccgagaaact ggcttttgaa caccgtttat caagaggttg
    17401 tgacacgtct tttgtcacaa cctcttttat ttttgcttca ctgtaactgc tacaatatca
    17461 ccgatttctt ttccgatgtt tttacgaata tcttttcgta gtccaaggat gtagcagact
    17521 gaaccatctg gatttttcac gcccatattc acgatactgc cttcatatgg cacattgtca
    17581 aaagttgctt ggactttaac tcttccagca ccgaattctt ttcttatatc atacggaaaa
    17641 ataatatatg ccccgccctt gccgacttcg gaagcataga tttttgattc aaaagaataa
    17701 atttccctca ttttatttct cctatttttt acatctagta tagcatagca aaaaaaagaa
    17761 ggaactgtga caaaagtctt gtcgcagttc cttttttcga ataaacggtg ttctcctgcc
    17821 aactcctcgg tcacaagtca caatagtcaa caaacatgag aagctgtttt ttgaaattgc
    17881 tccttgtgac tcagagttaa acggcaggct cacaacctct tccttaaacg gtgttcaaaa
    17941 gcttgcatct acggcactaa gcccaatcag atgaaaaata tggagtgcta tttttatctg
    18001 attgttctta ctgcttgatg cagagcagct ttttcacaac ctcctacatg gtgttcaaaa
    18061 tattattcgc cttttgcttc tttcactaat tttgcgaatg cttgttgttg gaattcagca
    18121 gctaaggctt tcgtacgttg ttcttgctct gctttgctca tttttggatt ttcagtcatt
    18181 gcttttacca taccgtcttg aacataagct tttgcttcac tttccatcgt actttgctgt
    18241 ttcttcacta attcagctaa cttggctgct tggtccgtac ttaattctac ccatgtctct
    18301 gggattttga atgtattcac tcggctgtca tattcgtgat tgttatcagc aatattgaac
    18361 cagaatttat acatgtcgtt tttgtattcc caataagtag ttttcgtcac tttttcagca
    18421 tcgccgtctg tcttttctac tttatttgtt tcgttatccg taccagttgc ttttggtttt
    18481 tcttgttttt catttgttcg gtaaagatac actttttctt ttccattccc taaagcttta
    18541 tacaaaagca tatctgctcc ttgggaatca gcactggaaa caattttttc tgtttttgtt
    18601 tccgttactt tttccattcc aaagtgattg gacaaattag cgacgatcaa acttagtgaa
    18661 gcgacgaaga ttagtccgaa aatcaaagat aaaaaggttt gccatgtttt tttagcgaac
    18721 acgttcgtta aagcaaaagc aagcaagctg acaatcaaaa ctccaataat catgcattat
    18781 cccccgcttt cattagaaca ggttcttttt tcttcaataa aaaggcaata ataaagccaa
    18841 gaatcccaaa gatgatcgct acgataaacg cggcatggta acctgacagt gtggcattga
    18901 ttgcctgatc tttataagct aatggcgtat tatccaatac agatttgctt gggatatttg
    18961 cttttgtcac attcgtcaac acactgatca aaatagctgt accaatcgag cttgcgattt
    19021 gacggaatgt attatttacc gctgtcccat gactgatcaa gttatttggc aatgcattca
    19081 taccagaagt cgttaccggc atcatgacca tggagatccc aaacatacgg atcgcataaa
    19141 ggatcacgat atataaaact ggagtcgttt ttgtcaagaa agcaaatggt gcagttgccg
    19201 ctgtcaaaat gaacatccct gtgatggcta aacgtttcgc gccgtattta tcgaaaatcg
    19261 cacccgtcac aggcatcata gcccccatga tcaatgcacc cggcaacaac atcaagccag
    19321 aatggaatgc tgattctcca cgaatatttt gaatatacaa tggtacgacc atttctgccc
    19381 ctaccatcgc catatttgtt acaccagcta aaacagctgc aatcgtgaat gtagatgatt
    19441 tgaatacacg aagttccaag aatggatgtt ccaaatgtaa ttgacgccat gagaacaagg
    19501 cgatgaaaat cacaccaaca actaagaaac caattacttt caagcttccc cagccgtcat
    19561 ctcctacact agaaaatccg taaagtaaac taccgaatcc aatcgttgat aaaatagcgg
    19621 ataaaatatc aatgctagga tttgacaatg gaataacact tttcattagg aagaatgcta
    19681 aaaccaagac aagaacaacg attggcagta ccataccaaa caagtcacgc caatggtagt
    19741 tatcaatgat ataaccagat aatgtagggc ctaaagcagg agcaagtcca ataacgatcc
    19801 ctgacatccc cattgctgat cctctttttt ctggcgggaa aatagatagc atgatatttt
    19861 gcaataatgg catcgatact cccacaccgc aagcttggat caaacgaccg attagtaatg
    19921 tactaaaatt aggtgctgta aagcaagtaa tcgttccgat caaaaagatc gtcatggcag
    19981 aaagatataa atttcttgaa ctgaatttat tgatcagcca tgctgttatt gggatcatga
    20041 tcccgttgac tagcaagaaa ccagtcgtta accattgcac atcagacgct gaaatatcga
    20101 aagctttcat caatgtagga aaagctgtcg tcaataaggt ctgattcaaa acggtacaaa
    20161 atgtaccaat taataagaca acaactaaaa gtgtccgatt atatggtttt ccgtaaatat
    20221 ctacgggttg gttgttactc atatttctcc ttctttctga attttttctg aaactactag
    20281 aatactttag tcctaaattc gctatttgtc aacctacttg acacataagt taatacggat
    20341 ataattaagg gaagcaagag gacaaaagga gtgacaaaaa tggttggggc ttcccttaaa
    20401 cgtttgttgg aaaatgatca attggttgtt ggcatcagtg aattaagtga tatcgtaggt
    20461 gtgtctccac gtcaattaag gtattgggaa caaaagggat tcattcgttc gatcgaagct
    20521 gatgcgaatt gtcctagaaa atatcgttta cctacagtca taaaagtaga actgatcaaa
    20581 aaatatttag atgacggatt tactttgtca aaagctgcag aaaaggcaga aatccgccaa
    20641 aagaaaatgc accatgtccg taaagttttt tcaaaagcaa tcaaagatat cgaattgatc
    20701 gacgaacgct atacgatttt acaaatcggt aacttcaaag aagagaatgc taagatgtac
    20761 atcattcatg acaacgagac agacgaatta acttacaagc tccttcctgt cgatgaaaaa
    20821 gctgactacg aacagttgtt aaaagaactc taaacgccac taatctccta agtaccaagc
    20881 tttttaataa gtagatgcta cataaatact tgaaaaaagg cctgtttgaa cataagaata
    20941 tccgaacggt gatctgcgtt tctttcatat agaaaggaat tctccatcac tgttcggata
    21001 tttttttatt tttttcggtt actttcttct aatacatctg ttagccaatt tgcatattct
    21061 gtttcccgat tgatcgcatg cgctaaaatc aggtagtgtc cgtaattttc atgtttttct
    21121 ttttcagtag gaaagatcgt tttttttcgc tctaatagat gaagtcgttt ttcttcatga
    21181 aggtcacgct gctgctgaat aatttctact aatgccggat cattcacgtc tttgacgaaa
    21241 tacaatttca gaacaaattc gtctcgatta accggtagtt cattggtggg tgtatgaatc
    21301 cactctgcca attgttcctt cccttcttct gttaattcat aaactttctt ttccaatttt
    21361 tcacctgtga tctctacttg atgctgaatg attccttgct cttccatttt tgcaagttca
    21421 ggataaatct gactatgttt tgcttgccaa aattctccta tttcattttt gaacgattga
    21481 ttcaattcat atcctgtcat cttcttctta cttaataacc ctaacaaaat atatcgaagt
    21541 gtattttttc ttgccatcag ttttccctcc ttgacaaatc tttaaatcca ttctatcata
    21601 aatgagtaat cgaaaacatg tagtttatta catgttttaa tttataagga gatgcttaaa
    21661 catgaaagaa tttaaagaat tggaagattt tattggaact cactttatct atacgtatga
    21721 caatggttgg gaatatgaat ggtatgccaa aaatgaccat actgttgatt accgtatcca
    21781 tggcggaatg gtcaaaggcc gttgggtaaa agaccaagaa gctgatatcg ttaaattgac
    21841 ggacggcgta ttcaaaatca cttggacaga gccaacaggg acagacgtgg cattggactt
    21901 tatgccaaat gaaaagaaac ttcacggaat gatctttttc cctaaatggg ttgaagaaca
    21961 tccagaaatc actgttactt tccaaaacga acatatcgca gaaatggaag cagctcgtga
    22021 aaaatatgaa acttatccaa aattacttgt tcctgaattt gctacgatca cttatatggg
    22081 tgatgcaggt atagacaatg aagatgttat tagcgaagaa ccatacgatg gaatgccaga
    22141 tgatatccgt agcggaaaat acttcgatga aaattataaa cgagtaaaat aaggttgtga
    22201 aagagaggtt gtgaaatcag cgttctgacc tgagtattag agaaagaaac ggaaaaatag
    22261 ttcctcatgt tttgccgttt tttgagctaa taccgagggt cagctgattg aacaccgttt
    22321 atcaagaggt tgtgaaatca gcgttatgct ttgagtaata agaacgctag ttagaaaata
    22381 gcttttcata ttttctaggc tagtgggctt attaccgaga agcagccgtt tgaacaccgt
    22441 ttatcaagag gttgtgaaat cagcgttttg acctgagtat tagagaaaca aatggaaaaa
    22501 tagttcctca tattttgccg ttttttgagc taataccgag ggtcagctga ttgaacaccg
    22561 tttatcaaag attgtaacaa ctgcatattc acacagttaa attagtgact tcaaaatgaa
    22621 ataatgctcc cagcaaaaaa ttaggctggg agcattattt cgttattctg ttcatcaaaa
    22681 atcagcgttt tgattccatt tcatcacaag ttcctcctca tttactgctt catctttttg
    22741 ctgtgtttga attgtaaaac cttgtgaaag atagaaatga atagctgatt gatttttttg
    22801 atagactgtc aaagtcagtg ttttattggt ttcttttatt tttttcatta gttgcgtacc
    22861 gattctttgt tgccggtatt cccttttcac aaagattcct gcgataaact gatcaatcac
    22921 cccagcaaaa ccaataattt cttgatctat ttctgctaag taaatcactg catctggtaa
    22981 agctttccta acagattcta cttgattctc ccaataagtt gatggaatga attcatgaga
    23041 atccaagtta gcatgcagcc aaatcgatag aacagattcc agttctttgg cagacaattt
    23101 tgttgttgtg cggatcattt tttctctcct tacttattca ttgattttaa aataaaaacc
    23161 atttttttga agctctttta caagttttcg cccctcaaaa aagtatatct tctcttgttt
    23221 tacttttcta ataccaaaag aaacagcaac tttaggatca tcagctgaaa cgattcgata
    23281 agttccacca ggcaattgat ccagcttgac tttcccatga tggtcgcttt gtactgaaaa
    23341 cggcacacca tttctagtaa gtgttttttt cttatgtttc aaaatgaatg aactaccatt
    23401 taccggtttc cctgacgaat cactaataat aaatgaaaaa tcaagcactt ttttacttaa
    23461 tctatactga tagattagtc gtccgataaa cactaacagt atgactacag ctattatcgt
    23521 tcctgcaaga ataaaccatt gccgcatggt tctttcctga tttccttttc cttcttcttt
    23581 ttttaccgat tcgttataag gaatccgatg tcctgtcact agcaaacgat gcgtgttgat
    23641 catatacggc gtacatgtaa gcaaggtaac taagtcttct ccttctgaaa tcgttagaac
    23701 agacgtgtca tctggcagta ccacttgtat atttttaact tgataagcca atttctcccc
    23761 caaaacagtc aaaataaacg tgtctccgtt ttctaaacga tctaattcgg taaataaccg
    23821 tcgattaggt aaaccgctgt gagcagagat cactgaatgc gtcgaatttc caccaagcgg
    23881 gaaagaggtt ccttgcaaga cgcctgctcc attttcaagg atcctaccat tgagccgatc
    23941 ataaagtgaa atatttaatt ggatcttagg tatattgata cttccgatca gccattcctc
    24001 taattggctg gatgttgtac tttcatttct gttcttttca ttgaaaggat cagtaccagg
    24061 attgaatcct tggtcagtta atcgttggtt tttctcctcc attttctttc gctgcacttc
    24121 aatttcagct gcggtccgtt tttgtacttg ttcgatacgt ttttgatcaa taaaactatt
    24181 taaagaatcc acataaaatg gatataaagc gatcagtaac cctacgacga tacatattaa
    24241 taaaaacaaa cgaaacaaaa gtcgacgaga ctgtttttgc ttactctgct tcacatgtgt
    24301 cctcccttgc aagttttttt gttctttctt ttattatatg ggttcaggaa aaaaatagaa
    24361 aatgaaagcc gatgaggttg tgaaaaagct gtcctgcatc aagtaataag tacagcccaa
    24421 aaaaatagct actcatattt tttgttgggt tgggcttatt accgcagatg caagcttttg
    24481 aacaccgttt aaggaagagg ttgtgaaaac tgcgttatgc tttgagtaat aagagcgcta
    24541 gtcagaaaat agcttctcat attttctagg ctagtgagct tattaccgag aagcagctga
    24601 ttgaacaccg tttaccgaga ggtcatgaaa aagctacttt gtttcgagta ttaagaaaaa
    24661 ttctgtttca aaacaaggag atgcaacaaa acttatgtcg catcctctct ccttagaact
    24721 ttcacttatg tttttcttgt aaaattcata aatattttca tgaattttct aatttcttgc
    24781 ctacctctcg cataggatat atgatgaatt tatctaaagt ttgcaaagga gttcgacaat
    24841 tgaaaaaata tggcccattt ttagcaattt ttttcattgc tttggcattt ctcatgcctc
    24901 atttattcac acataaacta attttgggtg ctgatgcccc attccattac aatcggttct
    24961 atgatgccgc gcaacaaatt aaatatgggg atttccatta ttttccttcc ctttatggat
    25021 tccagcaaag tggcaggatc gtgaatgccg tttacggccc atttttcgct tatttacaag
    25081 gattgatcgt gttgcttgca ggtagctggt ttcattatca gattctttca aatttcattc
    25141 tttatcttat ttcaggattt tctatgctga ttttactgag atatgtcaat gttcgaaatt
    25201 gggtaagtgt accagtagcc gttttattca tgagtacgta ttctattcaa tactggtcta
    25261 tcaaccaagg attcactagc tgggggactt gtttttatcc tttatgtatg atccctattg
    25321 ctgatttcgt ccttcataaa aagttccctg ttatcaaagt cgctgtctca gtgggactta
    25381 tgacacagat ccatttactg tcttcgatca tgttagcttt gatgtatatc cctttttatc
    25441 tttattactt ttttaaccag actgaaaaag gaaaagcact gcttcaactg attaagtcgg
    25501 tcttgcttta ttttgttctg acagcaaata tttgggcagg attagctatt gtttacagtg
    25561 ggaataagat tcttcctccc tttgttaaca gagaaatgag tcaaaaggcg atagatctgg
    25621 atggcagtta ttggctccat taccctaagg cctttcctat attgatatta tcaggttttt
    25681 tgctttttat tttctttcaa caaaagaaca ccttattcca aaaaatcgtg ttgtacacaa
    25741 gtcttgtttt cttttttctt tctactagct tgattccatg gacgacattg atcgaaaaaa
    25801 atgtgccttt tattagtttg atccagtttc catttcgatt tttcgtgcca tttacgctat
    25861 tgcttctact ttaccttgcc ctttcactga accagtggtc tgaacaaaag tggttccgtc
    25921 ttttaagtat atttggcatt cttatttttt cagcacagac gatccagaac ttataccagc
    25981 atctggaaaa gtgggaaaat gaaacatttg tcagtcgaca tacttatcta ttcgatacgc
    26041 cggaagaagc aaggaaaaca ttcttcagca aagatatgtc taccagcttg ttcgttttcc
    26101 aaaaaaccac acctgactat ctgccaatct ataaggagac aaaagacaat aaatacgacc
    26161 ggtataacga agaaatacta gaaaacgaaa caacttttat aaaaacacat ccaggcggtc
    26221 ttttgacaat caagtggaaa aacactgggg acaaaactgt tcatatccct gttatcgtct
    26281 acgatcggac tgtgctacag caaaatggaa aaacattgac tgattatgaa gtaacagata
    26341 tcgggacccc aatcgtgaag cagcaaaaag gaatcaacga gctcacgctt cattatcaaa
    26401 cgccaatcta tttctatttc attctttctc ttactttgat tggctggttt accttgctat
    26461 gtttatttat ttatcataga tacaagcttt tacgtgctta ggcaaaaggg ataaatacat
    26521 ccgatcaaaa aactctccaa acggtataat cgtttggaga gttttatttt aatcagtttt
    26581 tttatgttat atgttatatt ttttattcgt tcacatctaa ttcgatttcc accccgtagt
    26641 gatcactgat gattggacta tcgatcccat caaaaatccg ttgatatttt ttaacctgca
    26701 ttccttcagg gacgaaaata aaatctatac gcaatttttc tgtattttgt tcccaaccat
    26761 ctattttctt ttccacagta gctgcttttc ctttttcatt cgcgacaaca tacgaatctt
    26821 gaagaagaag gcaactttct cttacagtat aataaccttg agagtctact ggattattga
    26881 aatcacctag gaaaaaaagt ggtacgcgag tttccaaaaa gtagttttcc aagtttttcc
    26941 actcaaaata aaagccactt tttttatctg tccaccaaga aaagtggcag ctcgagacaa
    27001 aaatgtcctg atcatctatc tttgttttcc caatcaagat cttgcgtgtc tgataatctt
    27061 ctttcttttg aaattgggag acggtaagga cctgactttc caatgttttt ttcgtaagca
    27121 gtgcatttcc ttcttcatat ttatcataac cgatgtggct catttcccaa ctccaaaaat
    27181 actcttttcc gtgttccttt aaaagctgaa ctaaacggta ggcaaaatta tctttacgga
    27241 taggtacttg attctttacc ggacagaact tcattagctt cgcaggttct acttcttcag
    27301 attccagtaa ttggttgatt tcttgtaaag caatcacttc ataatcttct aacaggatct
    27361 tatctgctaa ttcttgcaat tttttctctg gttctggctc aagccagcta tgcgtgttga
    27421 gtgtcatgaa tttcattggt tttctccttg tcttcatttc gaaacaaaag ggttgtgaca
    27481 aaatttttgt cacaacctct ccagcaaata gtgttctcct gctaactcct cagtaataag
    27541 ctcaatggac caggagctat tttttgtttg gctgttttta tcacttggtg cagggcagct
    27601 ttttcacgac ctctccacta aacagtattc aaactgctgc ttctcggtaa taagcccaac
    27661 caaacgaaaa ataggaggag ctatttttgt ttggctgttc ttatgacttg atgcaggaca
    27721 gctttttcac aacctctcct tacattttaa tggagccgat cactttacca gctgtcactg
    27781 tacctaattg cttgatcact acttcttcta ctttatcgtt tgtaaatacg atcatgatcg
    27841 ttggatcttt ttcttcgttt tggatcacat caagatccat ctcagctaat ttcgttcctg
    27901 ttcctacttc ttggccttct ttgacagcaa tctcaaaagc tggctgattc atttggactg
    27961 tatcgattcc catatggatc aaaacttcga taccatcttt cgtttccagc ccaattgcat
    28021 gtttgctagg gaaaatactt gtcacttttc cagcgatcgg cgcaacaact tctcttgatg
    28081 ttggaataac agcaaagccg tcacccatca ttttttgtga gaataccgga tcttttactt
    28141 cagataaagg aatcacttct ccagaagctg cggaaactag ttcttgttct atacctaagt
    28201 attgcttact acctgcttgt ccatttcctg tagaaaccat ttctggctct ggaatctctg
    28261 ctcctgattg cagcagatct tcgatatctg atttcaagac atctgctttt ggtccataaa
    28321 ctgcttggac tccattgtct ttgacgatca atcccatcgc ccctgctcgt ttccaggctt
    28381 cttcagtccc tactttttct cgatcattta cacttacgcg taaccgagtc atacaagcat
    28441 cgacatcttt gatattttct tttcctccca gcaaatgaat gatctggacc acttgctgat
    28501 ctgctgttcc tgttccgctg ttagatacaa cggtttcttc actgtcattg tcgtaatttc
    28561 cgttacgtcc cggtgtagca aagttgaatt ttttgatcaa gaaatttgct aagaaatacg
    28621 taacaacacc aaaagcgatc acacacaaca cgaagttgat aagatctcca cctagacccg
    28681 ctttgatagc taacggtgtc cgagtcagca attcgatatt cccaaatgaa tggacccgca
    28741 atggcaagat atctgccatc gcaaatgcag ccccttggat cactgaataa atgacatata
    28801 atggcacagc agcaaacata aacatgaact ccaatggttc tgttacacct gttagaaaga
    28861 cagccaacgc tgctgaaaaa tacattgatt tatatcttgc tttcttatca ggatcgacat
    28921 tacgatacat cgcaaaagcc attcccatca agatacctga agaaccaatc atttgtccta
    28981 ctttgaaacg agccggcgtc cagttatgca acacaaactc atattgcgac atatcccctg
    29041 cacctttaag attcactaaa tcagtggccc aagccaacca taacggatct tgcccaaata
    29101 cttgtgtccc cgcctgtgcg cctgataaaa tctcatacga tccgccaagc tgtgtatagt
    29161 tgataggaat cgtcagcata tgatgcaatc caaatggcaa tagtaaacgt tctaatgttc
    29221 caaataaaaa cggtgctaga atcggtgcgc tttcttgtga ttctgcaatc cataaaccaa
    29281 aattattgat tccagcctgt acgtttggcc acaccacagc aagaatcaat gccacgattg
    29341 ttgaccataa gataacaaca aacggaacaa agcgctttcc attgaaaaat gacaaagcat
    29401 ccggcaattt tctataatta tagtacttat tataagccat tgccccgaca aatccagcga
    29461 tgatccctac gaatactccc atattcaaag ctggtgcttc caatacactt gtaaaaaatc
    29521 ctttaaccat gatcttcgta ccgaacagag tgtgggtaaa tgcatcttca ttcgcaagca
    29581 tgtcacttgt gacaccaaag atagccccag tgatccgatt gattaaaata aatgagatcc
    29641 cagctgcaaa tgctccgcct gctctttctt ttgcccaact tcctcctatt gctaaagcaa
    29701 ataaaatatg aagattacca ataattgccc aaccaatatt ttcaattact ccgcccgttg
    29761 tgacaagcgg cgtccaatca ggattgatca agggaattga tttccctata ctgatcatca
    29821 acccagctgc cggcataact gctaccacaa ccattaaagc ctttccaaat ttttgccaaa
    29881 actcaaaact aaatatcttt ttcattccgt ttagctcctt tacgcaatcg gtttcgttaa
    29941 acgtattata gcgtgcgttt tcttttttgt aaatgcttta ttttttattt ttctgtctat
    30001 accattctat aaaacctata atacgtgtgt tcccttctat aattttctcc taaaaattta
    30061 tttttttaag cttttttcta tgaaatattt tcttttgtct attgacttgt gcaatcggtt
    30121 gcgtttatta tgaacgtata aaacagctca ttaggaggag aaaaatgaaa cagatcaaac
    30181 gactatttgc tattgatcca tggaaaatca gcacgacaca attagataaa aaaaatcttc
    30241 gactgcaaga atctttaaca agtattggaa acggttatat ggggatgcgc gggaactttg
    30301 aagaaactta ctcaggggac caccatcaag gtacttattt ggcgggtgtc tggtatcccg
    30361 ataagacacg tgtcggctgg tggaaaaatg gttatccaga atactttgga aaagtgatca
    30421 acgccttgaa ctttatcgcg atagatattt cgatcaacga tcagctgatc gacttggctt
    30481 ctttggaacc agaagacttt tactgggaat tagatatgaa aaatggtgta ttatctcgta
    30541 cattcacaat cactacttct actaataaag tacgcatttc cttcgaacgt tttctgagca
    30601 ttgtcaaaaa agaatcagct tatatctatt taactataga aatgcttgaa ggtagcggaa
    30661 cagtcaaact cgtatcaaaa ttggatggaa atgttcaaaa cgaagacagc aactatgatg
    30721 aacatttttg gaatgagatt gatcgtggaa ctatagaaga aatcggctat ttgacgacta
    30781 aaactattcc aaacaacttt gggattgaac aatttactgt tactgcagca atgcgccatt
    30841 tcgttaatgg aaaatcagtc atccctgact atcacgaaga tactttagca ttacatggtt
    30901 gcttgtctgc agaattatct caaggtgaga catggtcagt ggaaaaagaa attatcgttc
    30961 ttactagtag agatattcca gaagcgcagc aagcagaaaa agctgtctca gaattaacga
    31021 accagacaaa caattatatc caagcaaaag aagagcaaac agcggcttgg gcagaacgat
    31081 ggaagcttgc cgatgtggtc atcgaaggag acgaagaagc acaacaaggt attcgattca
    31141 atttgttcca actattctct acttactacg gcgaagatga acgattgaat atcggcccca
    31201 aaggattcac aggagaaaaa tacggcggag ccacttactg ggatacagaa gcgtatgctg
    31261 tccctcttta tcttgcttta gccaaacctg aagtaactaa gaatctgctg aaatatcggc
    31321 ataatcaact gccgcaagca atccacaacg ctcagcaaca aggattaaaa ggcgcgcttt
    31381 atcctatggt caccttcact ggtgtcgaat gccataacga gtgggaaatc acattcgagg
    31441 agattcaccg aaatggcgcg attgcctatg caatctacaa ttatacgaat tacaccggcg
    31501 atacggaata tttgaaaaat gaaggactgg aagttttagc agaaatcgca cgcttctggg
    31561 cagaccgtgt ccatttttca aaacgagcaa ataaatacat gatccacggt gtgaccggcc
    31621 caaacgaata tgagaacaat atcaataaca attggtatac aaatacgatc gcagcttggg
    31681 tcttgcgtta cacgcgagaa aattatttaa cttatcaaaa agaagctgct atcactatca
    31741 gcgaagaaga actagccaaa tggaaagata tcgcagagaa catgtacttc ccagttgacg
    31801 aagaattagg aatctttgtc cagcatgaca cattcttaga taaagatctc atgcctgtat
    31861 ccgatattcc cacaagcgat cttcctctga atcagcattg gtcttgggac aaaatcctta
    31921 gatcttgctt catcaagcag gcagatgtac tacaggggat ttatttcttc aatgatactt
    31981 ttacaaaaga agaaaagcaa aaaaactttg atttctatga accaatgact gtccatgagt
    32041 cttccctttc gccaagtatc cattcgattt tagcagcaga attaggaatg gaaagcaaag
    32101 ctgtagaaat gtaccagcga acagctcgct tggatttgga taattacaat aacgacacag
    32161 aagacggctt gcacatcact tccatgactg gtagctggtt gtcgattgtc caaggatttg
    32221 ctcaaatgaa aactgcccat gatcagttga gattcgcacc atttctgcca aaaacttgga
    32281 aagcttattc tttccatatc aattatcgcg gacgtttatt gttcatctca gtcacagatg
    32341 aacaagttac tttcgagtta ttagaaggtt ctcctctaga aatttacgtc tatgaacagt
    32401 cttattcttt agcggatcgt ctagaaataa cgctaagaaa ggaggaagca aatgtttaaa
    32461 ggggtattgt ttgatttaga cggtgtcatt accgatactg ctgaattcca ttatcgtgct
    32521 tggcgaaagc ttggtcagga aatcggcatt acgatcgacc acacattcaa cgaacagcta
    32581 aaaggcgtca gccgagaaga ctcactctct ttgcttttag cttatggcgg aaaagaacac
    32641 tctttttcaa aggaagaatt tgcagaatta gcgaaaagaa aaaacgatta ttatttagag
    32701 atgatccaga cgatcgaacc aaaagatgtc ttccccgggg tcgtgccttt actggattct
    32761 ttaaaagaag aaaaaatcaa gattgctctt gcttctgcaa gtaaaaacgg gccattccta
    32821 cttgaaaaaa tgggtcttac accttacttt gatgctattg ccgacccggc agaagcaaca
    32881 aacggaaaac ctgcgccaga tatcttcctc cttgcagcaa aagctgtcgg actaaaagca
    32941 gaagaatgta tcgggatcga agacgcgcaa gccggtatcc aagccatcct ctcaagtggc
    33001 gctcaaccaa ttggcgtggg acgtgctgaa gaccttggag aggagatccc gcttgtccct
    33061 gatacgactt ttcttacgat cgattacttg aaagaaaagt ggcatgacca tggataatcc
    33121 agagatctcc gtcgtaccag ttccagagac tgaaataaag aaaatcacat tgaagaacga
    33181 ctacttagaa gtcgttcttc tcaattacgg tgcccgcctg caccaaatct tcgcaccaga
    33241 caaagaagga aaatcagaaa atgttttatt gtcttacgat cgtttcgaag atgttctaac
    33301 agacaaaagc ttttttggag caactgtcgg acctgtagcc ggaaggatcc gggatgcttc
    33361 ttggggtgag catagattgg agaaaaactg cggatcacac catatccacg gtggcacgaa
    33421 aggctggtct ttccaatttt gggaagtaga agttttcaaa actccatcat caataggtgt
    33481 ggtcttttac ttaaaagatg aattttctac ctatccagga ccaatcactg ctacgatcac
    33541 ttaccgttta acaaaaaacg aattagagat gacaacctct gctagtagcc ttgttgaaac
    33601 catctgcaat ccgaccaacc atgcctactt caacctttca ggaaatggaa caagagacat
    33661 ctatgaacat cagttaacgg tctttttaga tggtgtgctt gaattagatc aagaaaaact
    33721 gcctactgga aattggacaa aaaaagaaaa actgccaatc gactttagaa aatccccaac
    33781 actgcaagag atccttgcat gttatccaga tggactagat gatgtatttc ttttgcatca
    33841 tcctaggctt tccaaaactt ccttacaatt attcgagaag cattctggtc gccaaatgac
    33901 tatagcaact tctaataaaa gtatggtatt attttctacg actggattcg aagctgattt
    33961 ctctgtcaat ggaaaacaga tgcattcaaa ctacggatta gcaatcgaac cgcaagaaat
    34021 ccctgatatc gttcatttcc ccaaatttgg atcgatcaac ctccatcctg gtcaagaacg
    34081 gatcagtcag acgatttatc ggttctctgc acaataacaa gagaaaggtg tttttatcat
    34141 ggctattacc gtaaaagatg tggcgaaaaa agccggtgtt gccacctcga ccgtctctcg
    34201 tgtcatcaac gatcatccta gtatttccga gacaacaaag aagaaagtcc gaaaaattat
    34261 ggatgaacta ggttatgttc ctaacatgac tgcccgcaat ctaggaaaac gcatttccag
    34321 tgccatcggc gtaatcttac cccctcttga ttcaaaagag cggatcggta acccatttta
    34381 tcttgagatc atggaagcaa tcaacgaaga agcgcggaat tatgatatga ctacagcaat
    34441 cgctactgct aaaaattttg aattgctgtt agagaacgtc cagcgtatgc atttacaaaa
    34501 acaagtagat ggttttattc tagtgtattc agatagtcaa gatactgtca ttgattattt
    34561 ataccaaaac cacatccctt tcactttgat cggacagcct tatcatcatg agaacgaaat
    34621 cgtttatgta gataatgata accagcttct aggtaaacaa gcaacagaat ttttgatcca
    34681 gaatggacat aagaatatcc tgtttgtgac aaacacgact catgaaagtt tgtatttcga
    34741 acgatatttc ggttatcaaa aagcaatgat gatggctggt cttcctgcgt tatccagtgt
    34801 agtactagag catccggagg attatctgtc ctttgagaaa acgttgaaag aaaacgaagc
    34861 cacagcattg gtggtcattg acgatatctt cgcccttcgc acgatgcagt tggcgcaaat
    34921 gtatggctat gtcattccag agacactctc cgtcattagc ttcaacaact cgatcttctc
    34981 aacattaaca cacccttacc ttacaagtat cgatatcgat atctccgaat taggacgtgt
    35041 cgctatgcaa aagctgcgcg aattgatcca tgaagaattg agcagtggcg tacgtttggt
    35101 catccctcat cgcttgatca aaagagagac agtcgttcct ttagaaaacc catactaaat
    35161 agctgactca ctaaatacgc tgaccattta tcctatggtc agctttttat gtaaaagaag
    35221 atactacttc taacacattg tttatctgta aaaaagagcg tgggacaaaa gtaaagaaca
    35281 cttttgtctc acgctctaaa cacgtataaa cggcggaagc agaagcaact ccttcggaaa
    35341 taagctgaaa ttcacaaaaa tttaaaaagc aattttcttg agtttcttct tatttcttgg
    35401 agttaaacgc ttctgtccca gcctcttttt tccttccttc aattttttac acgtcgacga
    35461 ttcaataaaa aaccgcctaa tattgtaaag acaggtgcaa gaatcgagaa aaaggcgaat
    35521 ggcaagtaat gcaacgcgct aactttcatc gttcccataa tgaatgtgcc gctgactccc
    35581 cacggtacga gagaattgac ggtcgcacct gaatccgcta gtgttcgtgt caaataggtt
    35641 tttgataagc caacgtcatc aaaagaacgc ttgaatgttt ctcctggaag aatgatggac
    35701 agatactgtt ctccaacgat cagattgatt ccgatacagc tcaaggcagt aaacccgact
    35761 aaacgagaag gtcggtccat cttttctttt aattcttgaa cgatggtatc aacgatcaga
    35821 tatttgatca gtaacccacc taatgccaat gcaagtaaaa tcaaactagc agaacctaac
    35881 atactcatga tcccgcctcg agaaagcagt tcatctagct ttggatcagg tgtatttgcg
    35941 acataaccat tcatcaagat gtctgctgct tttccgaccg tcaaactttt ctcatggata
    36001 aaagaaagaa cgagagcagc tgtcgatccc gctagtaatg tagggatagc cggaattttt
    36061 ttgattgccc ccccaaataa aataactagt ggaatcaatg tccaaggaga gatccaaaag
    36121 ctcgctttca tgttttggat catttcattg atactagcta gatcggctga atgactgtta
    36181 tgtcctaata tcgtataaaa aatcaacgaa aggacgaatg ctgggaaatc cgtataacgc
    36241 ataccagcga tatgttcgaa tagattaact cctccaatac ccgctgccaa gttggtcgta
    36301 tctgataaag gcgagcagtt attccctaaa aaggcaccgg aaacgactgc gcctgtagtc
    36361 attgcatcat tgaatccaag taaatgaccg atccccaaaa aagcaattcc aatcgtcgaa
    36421 atcgttgtaa atgaactgcc gacagtcact ccgacaattg cacaaacaag aaatgctgtc
    36481 ggtaaaaaga aacgcacaga aacaagttta aatccataaa ccataatcgt agggatggtt
    36541 ccagataaaa tccatgaagc aactaagaca ccaatcaaaa gaaaaatcag gattggaatg
    36601 atccctggag tgattccttt gatgatcccg tcatggatct catcccatgt gaacttttta
    36661 aatctgccat aaaacaacaa acacatgaaa gctgcaagga tcgggatatg tggcgagagc
    36721 tggaatccga tgatcagtac tcctaggagt gccaacaaca cgatcaatac agcgatactt
    36781 tcttttaaag aaatattttt attcatcgtc gatctcctat ttcaaatgaa aaggccctgc
    36841 catgaaaaaa ctattcactt ggcaggacct cctactaaat cagcgacaaa acgtcggctg
    36901 aaagacttat ttttttattt ttcttctaaa cttttttcta cctcaatccc ccaagaatct
    36961 ttcttgaagt gattgatgat caatggaata acgaacatga ccactgtgat agctgctact
    37021 aataatgcat cactcaatgt cgctgtacct aatgtagcaa atacgacaac gatgatagag
    37081 aagatcagca tgcagctgac tacccaagct aatccgtttc cgtttttacc aattcggtaa
    37141 ggacgttcga attctggatg tttcttacgc attttaagca atgaaacagc aatcaaaatg
    37201 tacacgatac aataaataac agttgtagca tttgtaagtg tcaagaatac agcacttacg
    37261 tcatccatta caccataaag caaagcaaat aatgaaataa cgattgattg tgttagaacg
    37321 acatttctag atacgccgta tttgttttct ttatggaatc cgaattttgg tggaaggaat
    37381 ccttcacgag ctacttggat gattgttttt gatggtccag taacccaagc tgataattgt
    37441 aaaagcacac caataaatac catgaagctg aagatatttc cgatcacttc tggtaagcct
    37501 aggatttgac agtcaatcaa gataggctgc gtgatatttg ctaattccat ttttccatct
    37561 ggtacgacat ttgctacaag catcgcattg atgacattca ataaaactaa tccgatcaaa
    37621 gcgataaata cgccttttgt ataatttttt gttgcgtctt tcaaacgagg catgtaaaca
    37681 gaactcattt cgatcccaac aaaaataaat gtgataccag ctaagtattt caatgaatca
    37741 atatgttcca agtcagggaa agcttttgac catgaaaagt cacctagata gccgtttgag
    37801 actagtccga ctttgaaggc tgccaaaaca cctaaaacga acatgatgac tactgggata
    37861 tataccccta gccatacacc gatgttcccg ccgacttttg ccatatcgaa tttcaagttc
    37921 aagatcgtga tgatccagta gataactaaa atacaaccta aaacgaacca gtgattattg
    37981 ccaagttcta cattaccaaa tgtgtttcct aacaatggtc ctaaagtaga agcgaccatt
    38041 accatacctg ggaacatttg cacccataat agccatgcaa cgacaaatcc ccatttgctg
    38101 cccagtgctg tttttaccca taactgagga ccgccttctt gaggaagcat cgtggaaagc
    38161 tcaccggaaa ccattgatat cggaccggca aaaaacaaaa ctgcaaaaat tgaataaaaa
    38221 atcaatgtcc aaccgactgc ggctaacgta ggaatactcc gaaccgttgc acacagtgcc
    38281 attgtcagac cgataaaggt cccaagaccc attttactta cttcagattt actcaccgtt
    38341 gacactctcc ttgatttttt ggataaaata acggtgtctt tgaagtagtt tcagcaataa
    38401 acgaaacaat tcagcgaata tttgagggga ttctttgagt ttattgcttg aagggtttct
    38461 tcaaagacaa cctcaaaaca aagggatcga agagcttagg tctccccttg ctcttcagat
    38521 ccaaacatgc gtcagaaaca gttagtattc aagctgtttt ctaacatgat taccttgctt
    38581 attttgcttc gcttgccaat aattggtctg cgtagatttt ttccaatttt tcttgaagag
    38641 ctgctttgat tttttcagca tattcttcaa agtgttcttc tttgttcatg tatggtgtca
    38701 ttacgctggc acgcaataca gttacttttc cggcacggtt ccattcttca tctgagaatc
    38761 ctagttggtt cacaaattgt aatgggctgt tgccatagtc tgggatagca aagtcagtat
    38821 gtgaagttaa gaactcatta ccgtaaatgc ttcctttcac gtatgaagaa taatcatata
    38881 cgtcatggtt caatttattc attgcaacta agtcatcgtt gcctttttct ttgaatacat
    38941 agtctaccat attgaaatct ggataagtta atggatgaac ttcgatttct ttgtctccta
    39001 ctttaaatga caagtcattc aagaagttgt agaaacgatg tgcaccttcg attgatgcac
    39061 ccattaattt accatatcct gtgacattca aaggtaagac atggtgtgct gcccaaacgc
    39121 tggctgctgt tgcccctgct tttgaacctt ccaagatata agccccaagt aatgctggga
    39181 tatctgcgcc tttttcaaat acatatgtcg caaagtaaga gataacgtct ctcatgcgga
    39241 tatctttgat gacgatacca ccggcagaat atggaacata gcccatttta tgtgggtcaa
    39301 tcgttacaga ttctgcttct tcgattgctt tatatgcact gtgtacttct tctaagatat
    39361 aatctttgtt ttctgtaaag acattgtatt tgtaatgaac atcttttaaa tcttcaaatg
    39421 gaatgaagtt gttgtcttcg tctaagaaaa tcgcacggcc gtatccgcca taagcagcat
    39481 ctacgtgtaa gtagaagtaa ataccgtctt tttctaatac gcgacgcaat gcaacgattt
    39541 tatcgatacc gtcgattgct ccttcttctg ttgatcctac aacaccgact acacctagga
    39601 ttggtgtttt ttcagctgct agaccacgaa cgattttttc aagttcgttg atgtccatgc
    39661 gatagttgtg gtcaactggc actggaatca cttggtctaa accgataccg atgatgtcgg
    39721 cagcttttag ccatgaatag tgtttagttt gtggaaccaa ccattttcct aatttttcta
    39781 aatgttttcc gctacgtgct gagtgagctt tgatttcgtc gattttttct gggacgctgt
    39841 ctaacagatt catgatttct tctgttgaca agttcattaa ttcccaatcg ctttttccag
    39901 caactagttc aggtgtcact tctttcattg caagcggcaa tgatttgatg ttgcgggcat
    39961 accaaagtcc ttctaagttt gctaaagaac catcagcaac gatgtggccc cagccatctt
    40021 tatagctcat taattttgca aattccattc ctacttcttc ttccatttgg cttgttgcag
    40081 gagaagattc ataggcaacg ttattaccat tccatagcat agcaaaattg tacgctagta
    40141 aagatggcat caatgtttca gagttcatat gaccccagta acgtccggca ttatgccaag
    40201 gaacggaatg ggtacgcatc cgcgctgaaa tttctgatag aacatccttt gttttattca
    40261 cagtgtgttc aaaactagca ctgctttttt cttctggcgt aataataggc atgtcttgag
    40321 gcatgtagtt ttgacgccag cctaaatgtt catccaccaa ttcattcaac aaagctttat
    40381 agatttggcc gttctcagct ttgtccccaa tgaatagggc atttaggttt agatctttcg
    40441 acaatgattc actcatagtg aaaccctcct aaaaaaatat ttaaattttg tcgagcccta
    40501 cattacacct gttcttctat tctgtaaact aagttttggc ctgattttta atcatatttt
    40561 aatatgtatc aattatccga attgttcaca cttttgaaat aattaaaaaa aacgagggaa
    40621 tttacaaatg taaactccct cgcaaagatt gtattaacga caaccagcgt tctattttag
    40681 gatgggtttc aaaattggaa tgttgtaata gtatttccct ttttcaaggt aaaaacttac
    40741 taaattctaa tcaattactt tggccaaaaa gtaatttttc ttaccttttc gaataaccac
    40801 aaacttacca tcgaattcat cggatggatt tatgacaaaa tttaaatcgg taacgcggtc
    40861 accgttaata cttatagctc cgtttgaaac atcttcacgt gcttgacgtt tagatggttc
    40921 aatttttgtg gaaacaagca gttccacgat attttcaggt gtgcttgaaa tttcaacgtt
    40981 cggcatttta ccgaatcctt gcgcgatttc ttccgcattt agttctttaa tatttccaga
    41041 gaacaatgct tgcgttattt tttgcgcttc tttcaagtct tcttcactat gcacaaatct
    41101 tgtaacttct tctgccaaac ggcgttgtgc ttcacgtttc tcaggttctg tctctacttt
    41161 tttcgctaag tcttcgattt cttcttgaga caagaaagta aagaatttca agtatttcac
    41221 tacatcgcga tcatcttgat tcaaccagaa ttggtagaac tcaaatggtg aagttttctt
    41281 cggatcaagc caaacggcac cgcctgcagt ttttccgaat ttcgttccgt cagccttcag
    41341 catcaatggg atcgttaaac cgaacgcttc tgcttctggt ccttctaatt ttcggattag
    41401 gtctaatcca gctgtgatat ttccccattg gtctgctcca ccgatttgca attgcacatc
    41461 gtatgtttta tgcagatgca aaaagtcgat agattgcagg atttggtaag taaactctgt
    41521 aaatgagatt cctacttcca aacggctggc aacgatatct tttgctaaca tcgtgttgat
    41581 gttgaagttt ttaccgtagt ctctcaaaaa ttcaagtaaa gagatttttg acaaccagtc
    41641 gtagttgttc acaaatgtga tatttgcatc tttaccaaac aattttctca tttgattgga
    41701 aagtgcgtcc acgttatgtt gcacttgttc catcgtttgc aacacacgtt ctgttttgcg
    41761 gccgcttggg tcgccgatcg ttcctgttcc gccgccaatc agaatatatg gatgatggcc
    41821 tgccaattgg aatcttttca tcatcataaa tggaatcaaa tgtccgatat gcatcgaatc
    41881 accagttgga tcaacgccgc aataaagtga aatgctcttt tcttcaacga gctcgcgaag
    41941 tctttcttcg tttgtttgct gattaatggc gtcgcgccaa gttaattcat caataatatt
    42001 cattttactg acctccgtgt agttttttta tgaaagcagg aacctgctag catgtgtaat
    42061 taattatttc ttcaggatag gataggaaat aaaaaaagtc ccttggcata taagccaagg
    42121 gacgaaatct cgcggtacca cccaatttgt atgtctagat atccaacata cctctcgaac
    42181 atttaacgct gtcgcgtggc aatcactgcc aaagactcct gaaatgtaat tcgctttaaa
    42241 gcatgtactg gtttgcagcg accaccagct ttctgaaaca ttggctttaa caactacttc
    42301 gtttcgatct tcgtcttgtt attattaata tgtgtctatc atagagcgca actttttctt
    42361 tgtcaacaga aaaaggatat tttttttaaa ttcctcattt tttctttttt gaatccgctc
    42421 taattccgaa cattttattt tcaattattg ttaaaagaaa taactttttc tgtatttttt
    42481 ctgcaattta tcgagaagtc actttacttt gcctcttatt ctttcttcgt tacaataagg
    42541 aatgggacaa gtaaaaaaat gaaaaagcaa gggacgaggt atatggaaaa aaagcaaaaa
    42601 attattttag gagccacttc tgctttaact atcgttattt taattctaag tttgtttttt
    42661 gtcatccgat catctacaaa aaaagaacca atcattcctg aaaaaaaaat aacagaaaaa
    42721 gcaaaagaac cgacagatga taagcaacaa ctagttactc aagcaaaaaa tctggctcaa
    42781 agctacgatt atgaccaagc gattgcgtta ttggaaaaag atagtaagaa cggatctact
    42841 gaaacacaac agcttcttca aacactaaaa aaggagaaag aacaactgat cgactggtct
    42901 gatcctactc agatctctca tgtctttttc cacagcttga tcgtggaccc gcaaaaagcc
    42961 ttccactctc aacaggcaca aggatacaaa gattatatgg tgactgttga agaattcaac
    43021 cgttcgatcg aacaacttta tcaaaatgat tttgttttag ttaatttgaa tgatttgatc
    43081 caaaaagacg aacaaggaaa tctttccttt aacgggcttg cgctacctga aggaaaaaaa
    43141 ccattggttc tttcacaaga tgatgtcagc tattatgaat atatggataa ttccggtttt
    43201 cctagccgat tggtcgtatc aaataacaat gaaatcaaaa atgtctataa agagaaagat
    43261 aaagaagaaa tcggtgactt tgacatggtt cctctgatcg atgcctttgt caagaaacat
    43321 cctgattttt cttatcgagg cgcaaaaggt accttggccc tgacaggtta caacggagtt
    43381 ttaggttatc gaacatcaaa atctcaatat ggagacaatg aaaaaacaaa aaaagagatt
    43441 aaagaagcta aaaaagtggc ggaacagttg aagaaagacg gctggagctt tgcttcccat
    43501 acatggggtc acttgaatat gactcaagct tcactagaag atgtaaaaaa agacaatgaa
    43561 ttgtggcagc aagaagttgc acctatccta ggaaaaacaa atattctgat ttatccattt
    43621 ggtgcagaca tcagcgactg gcagccttat tcagcagaaa atcaaaaatt tgcttacttg
    43681 aagagtcaag gtttcgatat tttctgtaat gtcgatgctt ctacaccagc ttggggacaa
    43741 ttaggtacgg atttctatcg aaatgctcgg atcaatatcg atggcattcg ttttcaatct
    43801 gatttagacg gaaaaaatcc tattcttgat ccattcatca atgtccatga tgtctatgac
    43861 aaagaagcac gcgaatctgc ttgattttac gtcatttatg aaccaaaaaa gccgaactga
    43921 cattttatcg tcagtttcgg cttttttatg ctttatttct tcttcggtgc gattcccttg
    43981 atcaggactt ctgtccattc tgctgtatat ggaagcagtt ttgcgtatat ctccggctta
    44041 gaaattcctt ctaccattgc ttgaaaatag aggatcaaga actcatccga atatttcaaa
    44101 tccacttttc cttctttccg ccctcgatgg aaaagatcaa gcatgatact gaaactttct
    44161 tgcgtatacg catccatcgc gtcagaaaat tctttttttt ctttttttgt atagtatgtc
    44221 atcaaatcga tataaaattg tttgttcact cgattgagat aacggatttt gttttcactc
    44281 atggcaatca gtgtttcttc aaatggaaga ttttccgcaa tcaacgcttt cgcttcttgc
    44341 cctaaatcat tcataaatgc aaaaaatact tcataaatca gcccttcctt gctgccaaaa
    44401 tatttaaaaa tcgtcgcttt acttacctga gcgttctcag ctaccttatc cattgtcact
    44461 ccagcggttc ctatgtctgt gttcatcaag tcaaaagctg cttgtaaaat ctctttcttt
    44521 ttctcttgtg ctctcttttc aaaaccgttc atttgtagag cctcccgtaa ttttaaactt
    44581 ttataatata tatagttcaa atttattctg aggtttatta taactatcta ttgagaaagt
    44641 atcaagctag caattccttg tttatgaatt cattgacaga aatttataaa aagaatatta
    44701 tgtactaaaa taataaaaaa agttttataa aaaggtataa aaccttttta ttaaactatt
    44761 taatcttgtt tagtatatat ttttagaaaa aggagtgggg tttttatggc agaaatcgtc
    44821 aaagtcacac atttgaaaaa gagctttggt cgtttcgaag ctttaaaaga tgtctctttc
    44881 accgtacatt caggtgaagt cgttggattt atcggaccaa atggttctgg aaaatccacg
    44941 acgattcgga cacttttagg gattataaaa aaagacggcg gaacggcaac gatttttggg
    45001 caagatgtat gggaagatag tttatctatc catgaacgtc tttcttatgt tccaggcgat
    45061 atctctttgt ggggaaattt gactggagga gcgatcatcg atctcttcat gaagcttcat
    45121 ggaaaaggga gtgctcaaaa aaaagatgaa ctgattcaac gtttctctct tgatcctaag
    45181 aaaaaagcaa aaaactattc aaaaggaaat cgccaaaaag tgggcttgat tgctgctttg
    45241 gctgttgatt cggatctcta tttatttgat gagcctactt ctggtttaga tcctttgatg
    45301 gaaacgattt tccaagaaga agttgaaaaa ttgaaagaac agggcaaatc catcctactt
    45361 tcttctcata ttctcagtga agtcgagcga ctagcggata aagtcgtgat catacgcgaa
    45421 ggaacactcg atgaagaagg aacactcgat gaattgcgtc atttgactcg ttcgaccatc
    45481 actatagaaa cagaagcaga gaatagtcac ttagcacaat tgaacggtgt ccatgacttt
    45541 gtgcaaaaag gtcacctcgc tcaatttgct gctgataacg aagctttcga tgtgatttta
    45601 gctgaagcgt ccaaattagg tgtgaaaaaa tttgaatcta ttccaccgac tcttgaggat
    45661 ttattcatga gacattatga aggatgagga ggaagcgaaa tgactgataa attttcacgc
    45721 tggtccaccc ttttttctca aaaccttagc cgtgactgga aaaagatcct gatatggcta
    45781 gtcggtgtca ctgctttttc tggtggtttt gtccctgcgt ttgaagaatt gacttccgga
    45841 caaggattag ctggattata cgaaaccttg caaaacccgg caatgattgc aatggtcgga
    45901 ccaactccag cttcgaatgc tgctgagtat actttaggag cgatgtatgc aaatgaaatg
    45961 ttgctttttt gcagtttgtt tgctgctgtg atctctgttt tacacgtggt gagccaaacg
    46021 agaaaaaaag aagaacttgg tctgattgaa cttattcgtt cctttcgagt cggacgtcaa
    46081 gcaaactctt tagctgtcat cgctgaaagt attctgatca atttgctttt agcaatatcg
    46141 atcagttgta cgatgatttt tttccaagcg gattcgatca ctgcagaagg tgcttggctt
    46201 tttgggctat ctgtaggtag tatggggatt ttggggagtg ccgttgcact gtttgctgca
    46261 caactgatgc caagttcagc cagttcgaat ggtctgtctt tagcgttttt aggtggactt
    46321 taccttctac gagcaataac agatgtagct gattcctccc tttcgatgtg gaatccttta
    46381 ggctggtctt atttgaccta tccatttaca actaacaact ggattccttt gctttatttg
    46441 tttttgttca gtctaatctt gataatcagt tcatttgttt tagaggaaca tcgagatttg
    46501 aacaccggct atatccccga acgtgaagga aaaccgtatg ctaaacgttc tcttttatcc
    46561 ttgcgtggtt tattattcta tctaaataaa gggatgacaa tcagttggct tatcactttt
    46621 ggtttgatgg gggcggcata cggctctatc tacgggaata tgcaacagtt tttagaaagt
    46681 aacgacttaa tgaaacagat gtttatccaa tctggcgtct caattgaaaa aaactttaca
    46741 agcacgatca tggtcgtctt gatttgtcta gctgctattt taccaattgc tagcatcaat
    46801 cgtttattta cagaagaaac ccacggacgt atgaatcagc tgttcgcaac aaaaatttct
    46861 aggaaccaac tttattggac caatatgtta ctagctattc tatttgctat attggcaatc
    46921 ctgattagtt ctggtgcact agggctttct gctttggctt cgatgaagga tgcgaaagac
    46981 atggatctat tgatgtttct taaagccggg ttcaatttcc tgcctgccgt tttagtgttt
    47041 atcggaatta gtggctttct attcggatgg cttcctcgaa tcggtaaact tgcttatatc
    47101 tacttagctt actcctttgc actcaattac tttggcggaa ttttggattt accagaatgg
    47161 ttttccaaaa cagctgtccc tagctgggtt ccacggatgc caaacgattc ttttgatctt
    47221 tccgtgttcc ttacgttatc atgtatcagc cttgcactgt tcgtccttgg ttctattggt
    47281 tataggcgaa gagacttact agaaggataa taaaaaagca tgtacaattt catgctagcc
    47341 tccaaatctg ggtcaaaaat ccaacatttg gaggtcattt ttttatttac aagtcttcta
    47401 actgctcttt atcaatgatc cagacttctc gattctttct ttttacataa ccattctcct
    47461 ctaataattt aaattttcta gataaagttt cgggggtcgt accgataaat gcagcaagtt
    47521 ctttcatttt cattggcagg aaaaagtgat ccttccctgt cgctttagct aagtcaagca
    47581 gatagacagc gactcgttct tctacttttt ccatcatcaa aaaagttgtc tgctgctgta
    47641 cttttgttaa tttctcaacg ttgattgcta aaagtttcag actaagggcg ggataatgca
    47701 gtaagagttc cgagaaatct ttttgtctca atatgcaaat ttccgttttt tctagtgctt
    47761 cgccaaataa agactcattt ggaacgccta gcaactggct ctctccctca tatccgcccg
    47821 gctctattac tcgtaaaagc tgttcctttc cgctagcaga taattgatac actttcatac
    47881 tgccggaggc cacgatcact aattgaggtt ctccattcgg cgcaagaatc tgttctcctt
    47941 tttccacata gtgatgtttg acaagtgcgt gtactttttc ttgatcttgc tgtactaagt
    48001 gattgaacaa aggaacaaat gaaacacata aatgatcatg actcataatc tctcctcctt
    48061 ctaaacagct atttctaata aagaaacata acttccctta ttccaactga taattttctt
    48121 ttatcatagc ataaacgtca agcgaaccag ataccagcaa atagagtgga tttctttttg
    48181 tcccttgctt tattttcctt cttttgagac cttaagtaat tgattcctat caatttttta
    48241 aatatttact ttttgaataa tcaaaaacaa accttcactc gcaagccgag agtaaggttt
    48301 gtttttcgca ttgcttattt cgtgttgtct attgaaattt ttccgcacct agcttttgat
    48361 atagtggtgc tagttcttgt aaggtttcag cggttatttg ctcaacgtgt tcgatttctt
    48421 gcggatagaa gaatagttta cccatttgaa aggttcggtc tctcatcgct tttttcaact
    48481 cttcttgttt ctttgtatcg gacaaatagt cagacaaagc taagtggtcg tcaagttcat
    48541 gttctggttg cggccatatg taataatcct tgatatcgac tgttttgctc cactctttca
    48601 gtatcgcaag caactggtta tacgctgcaa tgctaccata tcgttcttcg cttttataat
    48661 gttggtacat caaatagatt tggtattgtt tttgattcaa caaaactgct agacaggcat
    48721 tctcgtcttg acgagattcg cttcggtacg cgctccaaaa atgactgcgt aaattccagc
    48781 cattcgtcca gctttcgatt ttaggtttgc tcatgccgta catatcaggc aaaagtgcag
    48841 ctgtctggag ctgaatctct ttccattttt gccagtgttc tttatattct ttcttgattt
    48901 gttcaatctt atctgttgga tggctctctt tgagctgttt gaatgcgtag attggtcgat
    48961 caaacaactg ataatctgat tttccaagtt ccattgtttc gcctctttcc gttatctttt
    49021 gtattctacc tgaattttgc gatatggaca aatagaggtt gtgaaaaagc tgttctgcat
    49081 caagtcataa gaacagccaa acaaaaaact gcgatgagca tcaccgcagt tttattgaac
    49141 ttaaggttga ttgatcacat ctaaaagcgt tgaaaataaa atggcactag aagcagctcc
    49201 tgggtcgata tgtccgattg cacgctctcc tagataggaa gctcgtcctt ttttagctac
    49261 caagactttg gtgtgttctt ttgcctggtc aatgatttct tttgttaaat tccctgcctt
    49321 caatgcctct gtgactggca gccaaacatc taacatcgtt ttatcgtcag cttctgcctt
    49381 tcctcggctt tgaatacccg ctgttccctg ctcgatcact tcgcctaatt tttcttgaga
    49441 atcaatagaa tcgacctctt ttgtcgcttt catcatgttc atgaatgcag atccgtataa
    49501 cggaccagaa gccccaccga ctttcgatat catcgccata gagaatactt tgaatgtatc
    49561 gctgattgtt tcaggatgat cggcttgaaa agcttcttca taagctgaaa cccctcttcc
    49621 catgttattt ccatggtctc catcgccaat cggggtatct agatcactga gatattgttt
    49681 gttctgattg attgcctctg caaattgcgt gagccattct tggatttctt gaacggttag
    49741 tttcatatta tcaccctttt atcttgagat tttttaccat gaaatcgtct gtacattgct
    49801 ttctaaagaa tctttccatt gtgtctccgt caagtcgatc agtgttagag aaagaccttg
    49861 catatctagt gaggtcataa aattaccgac ttttcggaaa actacattga cgcctctttc
    49921 ttccaacaga gctaaaacat cattcatgaa cacgaactgt tccatcaaag gcgtcccgcc
    49981 cattccgttg actagtacac ctgcttcttt tggtaaattc gagtaatcat ctaaaatctt
    50041 agtaacaagt tcctttgcca gtactttaga aggctgcatc ttttctcgac gatagcctgg
    50101 ctcaccgtgg ataccaacac catactcgat ctcatcttct cctagttcga atcctggttt
    50161 ccctacttct ggtacagttg ctgctcgaag cgctagaccg atcgtcttag ttgcctgtac
    50221 gaccttttct cccagttctt tcaactcagc tagggaagca ccgtttcttg ctgcatcgcc
    50281 cacgattttg tgtacaagga cagtacctgc aactccgcgt tttccagcag tatacgtact
    50341 attttcaacg gcgatatcat catctacgat cacactttct acttcaatat cgtccatagc
    50401 agctaattcc tttgccatat cgaagttcaa tgcatcccct gtataattct taacgatcag
    50461 caaaactcct tttccactat cggcttcctt aatagccgtc tgaatctgat ctggagttgg
    50521 agaagtaaac acatcaccaa gcaccgctgc acttaacatc ccttccccta caaacccggc
    50581 atgggatggt tcatgtccgc ttcctccacc agaaaccaaa ccaacttgtt ccgctttctg
    50641 attcttggct acaacacggg aagattctac acgatgaaca agttctggat agctttttac
    50701 taatccttcc agcatttctt cgacaatatt gcttggatca ttcatgattt ttttcaattt
    50761 attcccatcc tttctttccg gcattttgtg cttctgttaa gatttgctga gtatcatctg
    50821 ttataccggc agtgatggct gctgcaaaag ctccttccac taaaggcgca tccaccagat
    50881 gataacgttg ctgctgctct tcttccaaca tgtcttttgc cagttccgag ttcaataccg
    50941 cgctaccgat atccgcaaaa acaaaataac tagctgcatc gcttgattca ttgactgctt
    51001 ctacgatttt catcgggtct gatccgatct cgccatttat tccgccgaga gaaaaaattt
    51061 gcacctcttc ggatttttgc atctgctcaa tcatttcttt aataccatct gttacttttt
    51121 gactatgaga aataagcaaa atactttttt tcacaagaat cttcctttct tttacatagt
    51181 tgctaacgat tggtttcgct ttcattaatg aacaattcaa gtatataata aatttctaat
    51241 tttgggaaaa tatgtgtagt gcttcttgaa gtttgttata ctttaattaa aagagaggag
    51301 tgatcgaatg attacaacaa caacgaacag tgtagaggga aaagccgtag tagcttataa
    51361 agggatcgtt tttggtgagg tcattactgg gatccatgca tttaaagact tggaggcggg
    51421 gttgcgaaat atctttggcg gccgctcaaa aagctatgaa aacgaactaa tgggtgcaag
    51481 agaagaagca cttgccgaaa tggccgaacg tgcaaaagca ttaggagctg atgcgatcat
    51541 tggcgtaaaa atggactatg aagtattagg cagcgacaat ggcatgctga tggtcacatg
    51601 tagtggaaca gctgtctcca cgagataagt aggggacatc tgttttaata actaaagaga
    51661 gatgggacat gtagtggcac tcaagaatta gatttttcag tctaacttcg aggtactcta
    51721 ccttcccatc ctctcttttt tatttctttt tgcggatgat tccgtaagct gctgcgacta
    51781 acacacccgc ccctgctaag gaataactga cagaagctgt tttttcattt gtttttggca
    51841 aagtctgact tgttgatttg gatggctctg tcgtttgctg aactgtttct gtcgtgcttc
    51901 cagaggtcac tgatttttct cctgccgaat gcaaaatatc ttttattcgt tggaaactag
    51961 ctgctaagtc agcttttttt gcttcaaaat cattagcaac tgtttgtgct tcgctgatct
    52021 ttccgtggat tgcttgactg gccgctgtga tatccgcttg agtttgactt agctttccag
    52081 aagctgtttg agcaacctga tttaattgct gattgaattc ttttgcttgt tggatcttcc
    52141 cgtcaagatc tgtttggaca gcttccgctt ttgctttcgc ttcggcaagc tttttctcaa
    52201 gttgtgcacg gtcatttccc actttatttt taagatcagc taaatcttga tcgattgctg
    52261 cttggtcttc tttcaatttg ttttttgcag cttctaatgc ttcttcgagt tcttttcttt
    52321 tattttccaa gtcttgcaaa tgatcgattt ggtcagaaga ggtagaacta tttattgatg
    52381 tatcttctga agatgaaatc gtttctcctg aattatctgt cggtacattg gaagtcattt
    52441 ctgtttccga ggcaccgttt tcagcaccag aagagcttat ggttgttgaa ggttctgttt
    52501 gagcaggttc agaagtttcc gcaaaagctg tctgtcccaa taagaaaata ctggaaacag
    52561 caattggcca gattaacggc ttcattcttt ttttcatcac ttttcccatc cttactactt
    52621 tttaactaat ttcttattat aagcaaaaat gtttggaaga accataaaca caacaggctg
    52681 tttgtctaaa aaacggtgca tcttttattt ttttgtacgt tccgggcttt tccatgtccc
    52741 gcttccataa cctaaaatcg ttttgactgc tggaatgaaa gtcgtcacga tcgttatcgc
    52801 gttcactatc cagaacaaaa gcatatatag tggtgcgaaa agaaggtatt tcaattttct
    52861 tcgtcgatca tctacaatca gcgaagctag caactggaaa aatcctgcga ccatttcaaa
    52921 gcagatgaac acaaaactga atgtaaaagt aatataaatc tgttcgtagt ttccttgata
    52981 tacataatgc ccgattaatc cagcaaaaag aactacggaa atacaaaaga atattgacca
    53041 gacaatactc aatgtttgat caatgaagat gatcgttctt ccaatatgtt taaaaggatg
    53101 gagcatcact tttttgaaat tcgtcagcca gacttctgtt ccaccttttg cccaacgttt
    53161 tctgtgtcga tatagcatct tcaacgtcac aggtacttcc atgaaaaaga taatctcagg
    53221 agcaaaaaca gataaccagt catttaactg atgatcccaa gcaatactga tgtcttctgt
    53281 cgctcgatct tgtctgaatc cgcccacatc gatcaaggct tcttttcgat acatcgtatt
    53341 tgctccactg tacgcataaa gaccaccgaa aacagctgac tgtgttcgtt taatgatgcc
    53401 cacgatactt gagaattcaa ccgtttgaga ttttgcaatc aatttggtac gattttggac
    53461 atccatattt gcagtgaccg cagcaatatg tcttgcacca gggcgaatga aataattcac
    53521 atacctaatc agtgcatctg gctccggtac cgtgtctgca tcattgctta aaatcaattt
    53581 cccttttgca aaggcaagtc cgatattaaa cgcgtgggct tttcctttat ttttttcgat
    53641 ccgaaccaca cgaagattag cgtatttttt catcagtcta gctaatattt ctggtgtttg
    53701 gtccgttgaa ccatcgtctg tcactaatac ctcatagttg tgataattca atttcgtcat
    53761 caaatactcg attgtatatt cgatcacaat ttcttcatta tgggctggca ccatgatcgt
    53821 gatgaacggt tcgacactca agggaacatc tacccattct ttttgcttat gtttgtataa
    53881 aaacacataa caccagacac caataaacca tacaaatccg cctaaaatcg gataagaaac
    53941 gagaaaaaaa tacataatag acaaacaagt atctaagaat aaattacctg tatgggtgat
    54001 tgagatcatt tcagttccac cccattttct ttatacaaat ctgcaatgaa tgtcgtatct
    54061 agatttttct cttcggagac agaataaaaa cggaccgttt ctctctcttc ttttgttcca
    54121 aaacgttccg tatacacttc ttcaagtaa
//
LOCUS       DACDUU010000009        51444 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.009, whole genome shotgun sequence.
ACCESSION   DACDUU010000009 DACDUU010000000
VERSION     DACDUU010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.009"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..369
                     /locus_tag="IWL30_000574"
     CDS             <1..369
                     /locus_tag="IWL30_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HAP9705365.1"
                     /translation="AISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVP
                     QNVSKGVVFDRLESIYNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADY
                     VTVSNDKDGVSQAFERILTI"
     gene            650..1870
                     /locus_tag="IWL30_000575"
     CDS             650..1870
                     /locus_tag="IWL30_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAP9705366.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            1867..4587
                     /locus_tag="IWL30_000576"
     CDS             1867..4587
                     /locus_tag="IWL30_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9705367.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            4571..5515
                     /locus_tag="IWL30_000577"
     CDS             4571..5515
                     /locus_tag="IWL30_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9705368.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(5584..6594)
                     /locus_tag="IWL30_000578"
     CDS             complement(5584..6594)
                     /locus_tag="IWL30_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9705369.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(6774..7118)
                     /locus_tag="IWL30_000579"
     CDS             complement(6774..7118)
                     /locus_tag="IWL30_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP9705370.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7111..7545)
                     /locus_tag="IWL30_000580"
     CDS             complement(7111..7545)
                     /locus_tag="IWL30_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAP9705371.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            7683..8420
                     /locus_tag="IWL30_000581"
     CDS             7683..8420
                     /locus_tag="IWL30_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9705372.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            8413..9624
                     /locus_tag="IWL30_000582"
     CDS             8413..9624
                     /locus_tag="IWL30_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9705373.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            9831..10610
                     /locus_tag="IWL30_000583"
     CDS             9831..10610
                     /locus_tag="IWL30_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAP9705374.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            10680..11342
                     /gene="trmB"
                     /locus_tag="IWL30_000584"
     CDS             10680..11342
                     /gene="trmB"
                     /locus_tag="IWL30_000584"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAP9705375.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(11440..13194)
                     /gene="ade"
                     /locus_tag="IWL30_000585"
     CDS             complement(11440..13194)
                     /gene="ade"
                     /locus_tag="IWL30_000585"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAP9705376.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            13326..14255
                     /gene="cpaF"
                     /locus_tag="IWL30_000586"
     CDS             13326..14255
                     /gene="cpaF"
                     /locus_tag="IWL30_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAP9705377.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14212..15261
                     /locus_tag="IWL30_000587"
     CDS             14212..15261
                     /locus_tag="IWL30_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAP9705378.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            15258..15536
                     /locus_tag="IWL30_000588"
     CDS             15258..15536
                     /locus_tag="IWL30_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAP9705379.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            15511..15981
                     /locus_tag="IWL30_000589"
     CDS             15511..15981
                     /locus_tag="IWL30_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAP9705380.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            15998..16300
                     /locus_tag="IWL30_000590"
     CDS             15998..16300
                     /locus_tag="IWL30_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705381.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            16317..16688
                     /locus_tag="IWL30_000591"
     CDS             16317..16688
                     /locus_tag="IWL30_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAP9705382.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            16660..17019
                     /locus_tag="IWL30_000592"
     CDS             16660..17019
                     /locus_tag="IWL30_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705383.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17191..18198
                     /locus_tag="IWL30_000593"
     CDS             17191..18198
                     /locus_tag="IWL30_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9705384.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18223..19407
                     /locus_tag="IWL30_000594"
     CDS             18223..19407
                     /locus_tag="IWL30_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAP9705385.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(19844..21427)
                     /locus_tag="IWL30_000595"
     CDS             complement(19844..21427)
                     /locus_tag="IWL30_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9705386.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22005..22148)
                     /locus_tag="IWL30_000596"
     CDS             complement(22005..22148)
                     /locus_tag="IWL30_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAP9705387.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(22370..22849)
                     /locus_tag="IWL30_000597"
     CDS             complement(22370..22849)
                     /locus_tag="IWL30_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9705388.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(22922..23206)
                     /locus_tag="IWL30_000598"
     CDS             complement(22922..23206)
                     /locus_tag="IWL30_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705389.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(23284..23478)
                     /gene="ssrS"
                     /locus_tag="IWL30_000599"
     ncRNA           complement(23284..23478)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IWL30_000599"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(23550..24836)
                     /locus_tag="IWL30_000600"
     CDS             complement(23550..24836)
                     /locus_tag="IWL30_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAP9705390.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            24974..25645
                     /locus_tag="IWL30_000601"
     CDS             24974..25645
                     /locus_tag="IWL30_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAP9705391.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            25611..26099
                     /locus_tag="IWL30_000602"
     CDS             25611..26099
                     /locus_tag="IWL30_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAP9705392.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26112..27059
                     /gene="prmA"
                     /locus_tag="IWL30_000603"
     CDS             26112..27059
                     /gene="prmA"
                     /locus_tag="IWL30_000603"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAP9705393.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27060..27824
                     /locus_tag="IWL30_000604"
     CDS             27060..27824
                     /locus_tag="IWL30_000604"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAP9705394.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28057..30270
                     /locus_tag="IWL30_000605"
     CDS             28057..30270
                     /locus_tag="IWL30_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAP9705395.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            30281..30727
                     /locus_tag="IWL30_000606"
     CDS             30281..30727
                     /locus_tag="IWL30_000606"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAP9705396.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            30898..32157
                     /locus_tag="IWL30_000607"
     CDS             30898..32157
                     /locus_tag="IWL30_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAP9705397.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(32246..33193)
                     /locus_tag="IWL30_000608"
     CDS             complement(32246..33193)
                     /locus_tag="IWL30_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP9705398.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            33587..34879
                     /locus_tag="IWL30_000609"
     CDS             33587..34879
                     /locus_tag="IWL30_000609"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAP9705399.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            34894..36663
                     /gene="aspS"
                     /locus_tag="IWL30_000610"
     CDS             34894..36663
                     /gene="aspS"
                     /locus_tag="IWL30_000610"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAP9705400.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            36772..37647
                     /locus_tag="IWL30_000611"
     CDS             36772..37647
                     /locus_tag="IWL30_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9705401.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            37913..39796
                     /locus_tag="IWL30_000612"
     CDS             37913..39796
                     /locus_tag="IWL30_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP9705402.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(39871..40155)
                     /locus_tag="IWL30_000613"
     CDS             complement(39871..40155)
                     /locus_tag="IWL30_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP9705403.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40145..40879)
                     /locus_tag="IWL30_000614"
     CDS             complement(40145..40879)
                     /locus_tag="IWL30_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAP9705404.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41008..41631
                     /locus_tag="IWL30_000615"
     CDS             41008..41631
                     /locus_tag="IWL30_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAP9705405.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            41823..42944
                     /locus_tag="IWL30_000616"
     CDS             41823..42944
                     /locus_tag="IWL30_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAP9705406.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            42951..46079
                     /locus_tag="IWL30_000617"
     CDS             42951..46079
                     /locus_tag="IWL30_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAP9705407.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46098..49292
                     /locus_tag="IWL30_000618"
     CDS             46098..49292
                     /locus_tag="IWL30_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP9705408.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(49438..50145)
                     /locus_tag="IWL30_000619"
     CDS             complement(49438..50145)
                     /locus_tag="IWL30_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9705409.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            50910..51308
                     /gene="spxA"
                     /locus_tag="IWL30_000620"
     CDS             50910..51308
                     /gene="spxA"
                     /locus_tag="IWL30_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAP9705410.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT        16840 a         8993 c        10678 g        14933 t
ORIGIN      
        1 gctatcagca tttacaaatt ttttattttt attcctaatc gtcaattgaa agctaatata
       61 tttaaccatt tgaaaaagat gctaaatgtg tctgttactg aatcggcacc tgtaaatatt
      121 gaaatcgtac ctcaaaatgt atctaaaggt gtggtgtttg atcgtttgga atcaatctac
      181 aatttaaaga aacctttgag aatcgcgatc ggagatagtt taaatgactt atctatgttt
      241 gaaagtgcag atattagttt tgcaatggga aatagtcatc aagaaatcat gaaaaaagct
      301 gattatgtca ctgtttcaaa tgataaagac ggagtatctc aagcatttga aagaattctt
      361 accatataaa ctaattaagg tagtatagaa catagaattc taaatacgga aaaatggagc
      421 ataaattcca gtacaaatag tacttcttca gtgtcaaacg aagcgaatat aacatagata
      481 tttaggattg tgacaaaaat catgtcacaa tctttttttc gaataaactt attttttgaa
      541 gtcggccatt tatcgtaatt tctttgcata atacgaacaa attttcggtt attgatgaaa
      601 aaaaacattg aaaatggtat gataaaagaa aaaaaggagg aaatttttaa tggtgaaatt
      661 tatccatgcg gcagatttac atatggatcg gtcatttgaa ggactgacaa ctcttgataa
      721 aactgtacag gaaaaactat tgaagacgaa tctgaccgtt ttatcaaata tcattgaaca
      781 ggcaataata aataacgttg attttgtttt acttgcagga gataattttc atcaaaatcg
      841 tccttcatta aaaatacaga aacatttttc agaacagatg aaacggttag aaaagaacca
      901 tatacctgta tttataatat tcggaaatca tgatttctat caaaaagagc ggtattggtt
      961 ctcctttcct aaaaatgtcc atttatttac atccgaaacg gtagagacaa aaaaaataac
     1021 gataaaaagt ggagaaactg tctctctcag tggatttagt tatcgtcaac cgtggattca
     1081 aaaagacaaa gtgatggaat ttccttctag agaactcaca gattaccata ttgggttgta
     1141 ccacggggaa cctggcgtct ctcaaaaagg aaactatgct cctttccaac cctcaaagat
     1201 gcaagaaaag ggatacgatt attgggcttt agggcatatc catgtgccaa cggtcttaaa
     1261 tgaaaagcaa acaattgttt atccaggtgc gcctcaaggt catactaaaa aggaacaagc
     1321 ttctacatct attctactag ttgaattaag taaaggaagt tgccaaatac atccgattaa
     1381 agtagcagag gtctattgga agacaaagga aatatcattg aggaaagcaa gaacgacaaa
     1441 agaggtcatt gttcacattc gtcagcagct ttcaaaagta gaggatggat ttagtttaat
     1501 tgagataaaa ttaaaagatt atgaccatct caatacagat gttttggaac ggatacaatc
     1561 aggagagcta ttagactatt tgttagagga attctcagat aggataaatg atttctttat
     1621 ttggcgtatt tctctcatag agaatacttt ttatacgaag accccacttg ctgcttctgc
     1681 taaattgatg gaacagttat ttcaatacta tcagacacct caagatttcc aacaaatttt
     1741 aaatgaagta tatagtcatc aagaagcggc tagaatattg agcgaactgc cggaatatca
     1801 agaagaaaca cttgaaaaag ccaagcagct gcttaatcaa gattttttat ttgaggagga
     1861 tcaggaatga agattttaag gatcgagatt gctgcttttg gaaaatggcg tcaaaaaagt
     1921 tttgactttt attcaggcaa tcagctgatc tatgggggaa acgaagcagg aaaatcaacg
     1981 atttatcaat ttattcaagc aatattattt ggtttccctt caaaaggccg taaaaaaaag
     2041 gattatacac caaaagacgg ctcagcctat ggcggcaaaa tatggcttaa acatcctgta
     2101 tatggtgaat ttgctgtcga gcgctacaag cagcaaaatc gcggaaaatc gaaggtgtgg
     2161 ctgggggatc aagtgggaag tgatgagtta ctagaaaaac tgatcgcacc tttgacgaaa
     2221 gagttattcc agcaggtatt tactttccag caagagcagt tgatggaact agatcatttg
     2281 agagaaaaag agctacatga tgcgctgatt tctttaggca tcacgggcag ttctcaagta
     2341 ttccaaaagc ggcaagaata ttatcagaaa gcacaacaat tattcaagaa aaaaggccaa
     2401 aaattactaa tcaatcaaaa attaaatgaa tggaaagagc tgcaagaaaa aattcatcaa
     2461 aaacaagaac aggaagctta ttttggcaag ttgatccaac aagaacagga atacgagtcg
     2521 caacagcagc agttattggc acaattaaaa gaggcgaaca aacaattttc attagccaga
     2581 cagcaagaac ttaatttttc tttatatgaa gaatggcaat ctctcaaaaa aaacagcact
     2641 acaggtcttc ctgatgagca acttctttta gatctggagt ctttctccaa acggtatcag
     2701 caaacaaatg agcgtttgga agaattagaa gcaacactgg aaaagcatag cgggatggat
     2761 cagcagtcag ccagatatta tttttattta gagcaggagc aggaactaaa agatctccag
     2821 caacttcaat ttacagcgga acaattaaac gaagaatggc aaagattaca aattgagact
     2881 agagaaacaa attcagaatt gaagattttg gaacaaaggt ggcattggca gaaaaatccg
     2941 ttaccggaag aatttttata tgaagaagaa tggaagcatc ttctggctga agaaagaagg
     3001 attgctgatc tagaaagcca gatttctgtg cgtctccaaa tagctaaaga acagcaagag
     3061 atgattgaag cagaagtgaa tgacttggaa gagacctatc cagcattatt acaaaatcag
     3121 tcagagatag caccagtctc ttcatctaaa ccgtatatgc tgatggctgg cggagcaata
     3181 atcattgcag gtatatttgt accaatgccc ttgaaagtga tccttttact aattggtgca
     3241 ggcatgattg gctatttctt ctacaaaagg caaccaaaaa ttaaaactaa aaatgatttc
     3301 tctgagataa aaggaatgtg gcaagaaaaa ctaggacaac ttgatctagc cttagctcaa
     3361 ttagctgaag cagaagaaaa acaaaagaga actggagaag aaaaacagat tcttcagcaa
     3421 gaagtatatc gttttgcgga tcatcggcac ttaggaaaga tggatacgct caaactcatg
     3481 aaagaatatg gacaggaagt catagattat cagaaattat atggcgtatt tgagcggaaa
     3541 cataaaagag aacaggaaat acaagaaaaa ctacgacaaa tcgaccaaaa atttgagttt
     3601 gttaaagaat ggttaccgat acaagataag acaatttcag aaaaaatggt attgctttcc
     3661 cgttttatac aagagatgga acagatgaaa tttgccagaa gctatcagca aaatacttta
     3721 ttgaagcaag aaatcaatca attgaaaaag aaacaaaaag tatggataca agaatacagc
     3781 gaacaattga ctcaagcagg cttatcttat ccttcggaag tacctgtctt tttacaaaaa
     3841 gccaaacaag ccgaacagca gatgaaaaga tacaatgaat tatcggaaat gcttactcca
     3901 ctatttcctg aagagctgac aaaagagaaa ttatctgtaa gactgcgcca actgaaagaa
     3961 aagcaatccc aactccagca agaagaaaaa gaagcaagtg aaaatcggca acgactccaa
     4021 cttcaagtag aagagttgca aagagacggc acattggacg aactttatca acaagctagc
     4081 cggcaaaaag caaaattaca ggaattactg gaggaatggg gagcacttga aatcgccggg
     4141 acattcttag gtgatctttc aacagagatg agtgaaagac aattacccca gttgttaaga
     4201 gcagcaggtg attatttagg tattttaaca aaaggaaatt atcagaagat attactgaaa
     4261 aatgatacac tgactttaac agacggatat acagaatttc ccatctatga gttatctaca
     4321 ggtacgaaag atcagctgat tatggcgatg agatttgctt acctctcatt agaagctaac
     4381 cgttcgatat gcccgatcat cattgatgat ggctggcttc attatgatca tcagcgaaag
     4441 tatcaattag ctgaactttt agctaaattt ggagaaaagt atcaaataat ttgtttttct
     4501 tctgataaag aaatggtaag ctattaccat gagatgaacc aaatcgtaaa acaattagaa
     4561 ggagcaaata atgaaaaaaa ttcgtgattt agtaatcgat gagcagtttg aacaatttgt
     4621 cctgatcaaa agtgcagatg taagaatcgc taaaaacgga aaaaaattta ttgcttttac
     4681 tttccaagat acttcaggta ctatcgatgg taaatactgg gatgcttcgg aggaggaaat
     4741 caataaattt acagccggca cagtcgtttt tctaaacggt aaaagagagg tttatcaagg
     4801 aaaccctcaa gtaaagattc ttcatatgcg cttaacaagg gtagatgagc caggagagcc
     4861 tagtttatac atggaaagag cccctttgaa gaaagaagaa atggaagaag aaatcaatca
     4921 attgttgttt gagatcacca atgcgcattg gaatcgtatt gttcgctatc tacttaataa
     4981 atatcaaaaa caattttttg agtttccggc agcaaaaaga aaccatcatg catttgccag
     5041 tggattagct tatcatactt tgacaatgct tcacttaggg aaatcaatcg ccgatgaata
     5101 tcctgagctg aatcgagcat tgttgtatgc tggtatcatt ttgcatgatt taggaaaggt
     5161 gattgaactt tccggtccta tgtcgacaga atatacgctt gcaggaaatt tgatcggtca
     5221 tctggtattg attgacgaag aaataactaa agcctgtctt gcgttgaaaa tcagcgaagc
     5281 tgacgaagat gttcttgtat tacgtcatat ggttttagca caccatggct tattagaata
     5341 tggctcacct gtacgtccaa gaataatgga agcagaaata ttacatcaaa tcgacaatat
     5401 cgatgcttcg atgcagatga tgttgacttc gattcgtcaa acagaaccag gaaaatacac
     5461 cgatcggatt ttcggtatgg acaaccgaag tttctatgta ccaaaagatt tatagcaaaa
     5521 atttattttt ttcattcaaa acaaaaaaag cgagacataa gtcttcgctt tttttgtttt
     5581 cttttatttt gatgaatcag ttgtttcttc agttgaagat tcttttgttg aatcagttga
     5641 ttttgtgtca gaactctttg ttgaagctgt agtttcactt gagtctttcg tacttgatga
     5701 agttgttgtg aatgcactca acacattttc aaaggcatca tctttgattt ttacatttgc
     5761 atcttttaat tcttcaccga ttactttagt tgtgaatgca ttgtcactta gtttagtttc
     5821 agtagcgata tcttttagtt gatctttgta tttatccatg tcattgcctt tattttggtt
     5881 tttcaccatt ttgacaacgt agtattctgt cgcataagaa gtaggatttg ttgtagtaat
     5941 aacatcggag atctcaccgt ctttcaattt gaaagctgct tcttttactt ctgcaggaat
     6001 agttgttgtc gttgagtcga atttaacttt accgccatct tcttttgtct ctgtatcagt
     6061 tgatttgtct ttggctaatt tagagaaatc atctccatca tccgctgatt ttttcacatc
     6121 ttttgcttca tcttcacttg ataatttgat gatttgtgct tctacttctg gatggaaaga
     6181 tttccaagca gttttcaaat cgtcatctgt aatatctacg tgagctttca agccagcttc
     6241 aaaagctaaa ttgttacgaa tatattcttt ataagtatct ttcgtatatc cagcagcttc
     6301 taattgtgat tcaaaagtat cacctaatga tttcgcttgt ttgtcgtatt ctgcatccac
     6361 ttgcttatcg gttactttat cgccatattt gttattgaat actttataaa tgatcatccg
     6421 ttgtactaag gattggttcg atgattcaag ttttgcttca tcatagaaat cagatacagt
     6481 gatcgttccg cctttcatcg ttgcgatatc tttattagta tcgcctgaac aagctgctag
     6541 agttaaaaca gccaacgcac tagtagctgc caaaataatt gatttttttt tcattaaaat
     6601 ttgcactcct gtaattagtt attattttta tatattaact ataccatatc ccatttcctt
     6661 taaaaacgtt gtcgtggtag tttcaaacaa aattcaaaat ttcatcacat tcctaggaaa
     6721 tacgcaaact tttctcactt atatagttaa atctgttgag aaaataatca ttattatatt
     6781 tgttcaggat actctggcag atcagctttt agtttttcta cttgttcatt gatttgtgtg
     6841 atacgtggtt cggcttggaa tttaaaatct tcgatatctt ttttgattgc ttcttggaaa
     6901 gcaggaatca cagattcagc tgtttctttt gtttcgatca atgcgctttt aaaatgattc
     6961 aggctgttat ttaaatcatt tgtcagagtt gttgcttctt ccaattcttc gatcagtttc
     7021 tttcttgttt cattcccgct cctgggcgcc aataacagac ctccgacagt gccagcagca
     7081 gctccaaata acagtccttt gaaaaagttt ttaatcatcg caggacacct gctttacgat
     7141 tgtttcttga atagctttta gttcttccga tccataacta tcttgatgat tgacaaagtg
     7201 aatagaaaaa tcatcctctt tgctgtagcg agggactaaa tggatatgtg aatgaaaaac
     7261 agattgatag gctagttcac ggttgttatt caagatatta agacctttca tttctggaaa
     7321 agctttttcc aatgcttgag caatcttagg cacacgcgaa aaaacctctc ctgcaagttc
     7381 gggatcatat tcaaaaatat ctgctacatg acgtttaggc acaactaatg tgtgtccttt
     7441 cgttgcttga gagatgtcta aaaaagcata gaccttgtcg tcttcatata ttttatagct
     7501 tgggatttct tgattgatga ttttgcaaaa aatacagttt tccatataaa acatcctttc
     7561 tataaagaat aaatacttca gcttaacttt accataagtt aaagctattg taaggaaaaa
     7621 aacaaaatac cgtcattttc ttcgtggtat aataaataaa aataaatggc ggaggaaaaa
     7681 gaatgagttt aattatcgat catgtcacag gggggtacgg acatctccct gtgttaaaag
     7741 atatcagttt cgatgtacag cctggagaga tagtagggtt gattggactg aacggagcag
     7801 ggaaaagtac gaccatcaag cacattatcg gtctgctgga agcttcaaaa ggaacgatag
     7861 aaatcaatca tctatcctta aagaaaaata cggaagaata tcgcaaacaa attgggtata
     7921 ttccagagac tccttcttta tatgaagaat tgacattgag agaacatatc gaagtcactg
     7981 cgatggccta cgatatacct atggatatag caatgaaaag agcagaaaag ttgttggaga
     8041 cttttcggtt atctaatcgt cttgactggt tcccagctaa tttttcaaag ggaatgaaac
     8101 agaaagtgat gatcttatgc gcctttttga tcgagccttc tttatatatc atcgacgaac
     8161 catttttagg cttggatcca ctagctatca atgcattgct tgaattgatg gtagaaatga
     8221 aaaaagaagg agccggtatt ttgatgtcta cgcatatttt agctactgca gaaaaatatt
     8281 gtgatcgttt tgtggtctta cataacggag agatacgagc agacggttct cttgagatgt
     8341 tgagaaaaga atttcagctg ccggattctt ctttggacga aatctatatt gctttaacaa
     8401 aggagcaggc acatgagtag tttttttcaa aaacgtttag caagacatca aaagaaaatg
     8461 atgaaatata tgcgttatgt attgaacgat cattttgttt tagtttgtct tttcttgatt
     8521 ggcggtgtgg gattatatta ttctaattgg ttgaaaaccc tgcagccacc gtttgcaatt
     8581 ggagggctca taatctctgt attttggatg ctctgtttat tcgtgggtaa gatggctact
     8641 tttgcagagg cagcagatat tgttttttta cttcctaaag aaaaagaaat gcaaacctat
     8701 ttggaacgag cttttcgcta ttcttgcatt tttccgttga tctttttgat attggcatgc
     8761 ggattggcca tgccattagt ggtggttagt acggagcaat ccttttcgat gtttttcgtc
     8821 tatctgatca tattatggag tttgaaattc agtcatctgc aaatcaagag agcagaattg
     8881 tttcgactag atcaacaagt tattcgttat tggaaattag gctggttttt ttcttctctg
     8941 atctttttgt ttctatcatt ttattggcta atatgggcag gaatagcagg tgcattagta
     9001 caggcaagtc tatattactg gtttttgtgg aagcaacaag aaaaaggatt ggattgggag
     9061 aaaatggtgc atgtagaaga acataggttg catcagatct atcaatttat caatttattc
     9121 acagatgttc ctgaaattac ggcaaagatc aaaagaagac gctatttgga tcctatattg
     9181 actcatatta aaagagaatc aaaaaacact tatctttact tgtttgcaag aagattggca
     9241 agaggttctg attttagtgg tctttatttc cgattagtaa ttataggtgg gttattgatt
     9301 gccggagtag tcgactttta ttttagtctg attatcggtt tcttattttt atatttgatc
     9361 ggtttccaac tgttaccttt agcgaaccag ttccgctata tggttctcgt tcagctttat
     9421 ccaatccata agaatcagaa gaagcaggcg attcagtatc tactcacatg ggtattaatt
     9481 atcgcgtcgg ttatttttgg tttgattgct gcagtcgtat tagatgggca gcagcgatgg
     9541 gttccgctag cggcttattt gattgttact atgctctttg ttaaactata tgtaccaact
     9601 cgtttgaaga agatgtcaga ctgaaaacaa agctgtgtag ggtaaaaatg agaatgaaaa
     9661 atagagcgtt gacagtaaga atgctgttcg ttctattttt tttgagaaaa gctggttttt
     9721 tgaatggcaa ttaaatgaaa aagaacgtat atgtgcttga cgtgtagtgt tcaggcgact
     9781 agaataataa gtactgcaaa acaaagagta agaggacggg agaatgcatg atggagtttc
     9841 agttagacca agagtggcgg ttacgtccca tcaaaggcga cacaggaaaa acatacatag
     9901 gcatgcgtga tgaggacaaa gtctttatta aacgaaacac gactccgatg ttggctgctc
     9961 tttcaaggga aggaattaca ccaaaacttg tttggacaaa acgaacgggg aatggcgata
    10021 ctttgacagc acaagagtgg ctggaaggtc gcttgctgac tcctaatgag attggtcaaa
    10081 gaaacgacgt gattgatgtt ttgtaccatt tacatcattc aaattctctt aaaaccatgc
    10141 tgacccgtat cggtggtcgt gtcatgacgc cggagaagat gctgcaaaaa tatgaaaaag
    10201 aattaccgga agaactccag aaaaatcaat ttctgcaaat tgtccatcgt tttcttcgtg
    10261 agcagatccc gtcaatagat aaggaaaact tatcagtagt acacggagat gtaaaccatc
    10321 gtaattggtt agtatgtaaa aattatttat atctagtcga ttgggattca atcatgtttg
    10381 cagaccctgc ggtggatatt gcaacgattt taggaaatta tgtgccactg tctagctgga
    10441 atcagtggct gatcagttat ggcatacgcc cgactgacga aatgcttgaa aaatttcatt
    10501 ggtatgcggc catgaatttg ctccaagaaa tcaccagata ttgcaaacgt gaagatcatc
    10561 gacggatgaa cgaagaaatt ttacaattaa aacgaatttt cagtggctga ggtttggccc
    10621 aaactctgcg gagatttggc cagacctttt taattagagg aaaggaatgg agataaataa
    10681 tgcgtgtccg aaatcgtccg ggagcagcgg aaatgttagc ggctcacccg aattttgtaa
    10741 ttagtgatcc aacattgtgg aagggaaaat ggaatgaatt gtttgaaaac gatcatccaa
    10801 tccacattga gattggtatg gggaaaggcc agttcatcac tggaatggca aaagcccatc
    10861 ctgaaatcaa ttatattgga gtagaaatgc aggtcagtgt tgtttcgatt gcactagaca
    10921 agttgatcga acaaccgctt cctaacctga aacttctaca tgtggatgga tcagcgttga
    10981 cagagtattt cgctgacagc gaagtagatc aaatctatct gaatttttcc gatccatggc
    11041 caaaaaaacg tcatgaaaaa cggcgattga catataagac ttttcttgct gtagatgaac
    11101 agattcttcg tccaaatgga gaaatccatt tcaaaacaga taatcaagga ttgtttgagt
    11161 attctctggc aagtttttct caatatggca tgatcctcaa acaggtatgg ttagatttac
    11221 atcaaagtca atttgaaggc aatattatga cggagtatga agaaaaattt tcttcaaaag
    11281 gtcaacgtat ttatcgagta gaagctcaat ttcaagataa gaccaaaaag aaaagtctgt
    11341 gaaactaact ttctgctttc ataaaaacat taatatccat aaataaatct cagaaaatga
    11401 acattacaga tgtaatgtat tttctgggat ttatttgatt taaaatgtat ttatttcatc
    11461 ggtttttcca caaggtggaa tcaattggaa gcgagaaaat gaaaataatc ctttgtcggt
    11521 gatcttcaat tcgggtatga cagataatgt taagaaagaa agtgtcaaaa aaggatcaaa
    11581 ggttttagct gctcctagtt gatgtgcttt agttattaat tcattcaact gttcatagac
    11641 gatcgaaaaa ggttcctgtg tcatgattcc gcctatagga agagaaacac aagctatttc
    11701 ctgctgtttg ttgacaacgg tcatcccgcc acctttttga atcaaaagat tagcagcata
    11761 cagcatatcc tcgtcgtttg tcccaacgac aactagatta tgcgagtcat gggcaatcgt
    11821 tgtcgctatc gctccttgtt tcagctgaaa tcctttcact atgcctaatc caatgtttcc
    11881 cgtttgatgg tgacgctcga tcaccgcaat ctttaattgg tctttttcga ttgaaggaat
    11941 gaatcgttcg ccttgtcggt ccactttttc taccaaatct ttagtcacta gactattcgg
    12001 gataatttcg atcacatggg cttgtgaaga agaaagaagc agctctaacg aatgtggatt
    12061 taatggttga gcgtttagtt ttggcagaca acacgtatat gaatttgaat gatcagcaaa
    12121 taaatggtct tggagttgcc cagattgaac aactaatgtt cccttataga aaacttcatg
    12181 aattggcagc ttggtaagat cgtctgttaa gaagaaatca gcttgatacc caggggcaat
    12241 cgcacctagt gttttcaaat caaaacattc tgctgcattc aatgttgcca tctgaatggc
    12301 ttgtaatgga tcgattccaa gtgcaatagc ttgacggacg atatgatcga ttgatccttc
    12361 ttttactaaa tcattgatca gtttatcatc ggtgacaaag aagcatcttc ttgaattttc
    12421 aggtgtgatc gccggtagta gcgatgccag atcttttgca acggtccctt ctcgaaccat
    12481 taaatacata ccaagatcca aacgttcttt agcttctttt gcagttgtag cttcatgatc
    12541 cgtccgaata cctgctgcag gataaacatt taactcttcc atgccgatac cagctgcatg
    12601 cccgtcaatt tttttcttat gctggtgcat caattggatc ttttctagga tgtctgtttc
    12661 attatttgct actgcttgat agttcatgac ttcagctaag cctaaaactt cgggtttttg
    12721 taaaaaaggg gctaattttt ttgcatttaa atttgcacca ttggtctcaa aaggggttac
    12781 aggtacacaa gaaggtagca taaaaaagat attcatgagt gtttgtcttg catcttctat
    12841 cataaactca agacctgtac tcccagcaat gtttcctatt tcatggggat ctgtcactac
    12901 agtagtaact ccatgaagca aggatacttt tgaaaattcg cttggtgata aaagtgagct
    12961 ttcgatatgc acatggccat cgatgaatcc tggcactaga tattgcccat tagcattatg
    13021 tacgcttttt ccttcgtatt caccgattcc tacgatcatt ccttcgcaga ttgctacatc
    13081 tttttccatg atttctttcg taaagacgtt gacgatctta gcatgcttga tcactaggtc
    13141 agcaggcttt tttttacttg ctacttcgat atatttcctt aatgttgttt ccaatggagt
    13201 gcctcccaag ataaattttt ataagtataa cataaattcg gcttttgcca atattttttt
    13261 gtaaattcat tttatttgaa acaaaaagat caaaaagacg tatgtttttt gtggagggga
    13321 gaataatgga aataaaacga ttatcggatc aaatgataag tgaaagtttt gccaaacatg
    13381 cccaagacgt atatatattg ccagcttcag aagtatttta catctatttt cgctatggaa
    13441 aaaaacgaac attctggaag aaagtctctt cggcagatgg acagcaactg atctcaagat
    13501 ttaagtattt gggacagatg gatatcggag aaaaaagaaa agctcagtta ggtgcattta
    13561 cttattcttt agaaaaacag gaagttcgtc tgcggttgtc ttctgtagga gattaccttc
    13621 aacgagagag tttagtgatt cggctattat acggtacttt tgaaacaccc catcgttgct
    13681 ttagttctcg agattttgca actattttac agaaaaccgt cagacgtgga ttataccttt
    13741 tcagcggacc agtcggatca ggcaagactt ctctaatgta tcggttggct ttggaagaaa
    13801 aattgcaagt gattactatc gaagatcctg tagagataga agaaccgttt tttctgcagc
    13861 tacagatcaa tccgaaaata gcacagactt atgatacact tttgaaactc gcattacggc
    13921 atcgtccaga cttgctaatc attggcgaga taagagatga atcaactgca catgctgcgg
    13981 taagagcagc actaacaggg caccgggttt ttgcaaccgt acatgcacgc aatcttgaag
    14041 gtacattggt cagaatccat gaactgacaa agaaaaaaga agagctatct gaatgtttag
    14101 ctggtgtcat ttatcaagaa cttttggaag atgccaatca ccatccttct gttttatgga
    14161 gttatcaatt tatggaggaa gaatcagtaa aaaaatcatg ggaggaaagt tatgaagaag
    14221 ctcaggaaaa tatttggcag tcagaactat tctaaaggat taacaagaag ccagcaaaac
    14281 cagtttgcac aaaccatggg agaactgctt ttatctggtt ttagtctgca agaatccctt
    14341 tatttattac tcacgatccg ttcgtttcca cctgttattt tggcaaatat ccaacatcag
    14401 ctaaaggaag ggcaagcgtt tagtgaggta ctttcgcaaa taggatatgg agcagagcaa
    14461 ctggtacaga tcgagttagc tgaacgtcat gggaatctgc cgacaacttt gttggaaatt
    14521 gccaaacagt ttcggcttgt tgaaggattt cgcaatgatt tgaaaaagct gcttgcatat
    14581 ccgatgctat tgctcacctt tttgctaggt atcttgttta gtatgcggtt gttcgttttg
    14641 cctcagctta ttggttcagg aatggtcatg caagagcatt ggggtattca gctgatacaa
    14701 gtctttcatt ggtatgttct actattcatc ctagcggtta ctctattggc tttactagta
    14761 aggtatcgtc tgatgaagtt gtctcctgtt gatcagagtg aatggataag cagacaatta
    14821 ttgattggac cgatatattc cttgtatcaa tcggcttatt tgtcactaga gattggaaag
    14881 ctttttcatg aaggtttcga attaaggcaa atcatagtct gcttaaagga aacgaaacaa
    14941 gaaagtctga ttcagcatct tgcctctaag ttgattcaag gattggaggc aggtatgtca
    15001 ttggctgagc agttccagaa ttatcgtttt ctatcagaag aattcagtaa aattgtttta
    15061 cagggagagg caaagggaag tttaggtaaa gaactgattt tttacagcga atggacaaga
    15121 aaacagcttt tcaaacgtct tcatcactgg atgcatttta ttcagccaat catttttttg
    15181 ggcgtagcgg ctttgatttt attagtttat gccgctgttt tattacctgt ttacggaagc
    15241 atagaggagg ttttgccatg aaaaagagaa aaatatctta ttatagcggt tttacattga
    15301 tcgaaatgct aatcgtctta ctgatcatca gtgtattagt gttgttgttc gtccctaatt
    15361 tgtcgcgtta taggaatcat gtagaccaag aaagtcgtga agcgatcatc cagttggtag
    15421 acacacaaaa agagctctat gcgcttcaaa ataatggaag agttcctact gtggaggaat
    15481 tgttgaatga aggctatatc aaacgagaac atgcagaaat ctatcagcgg ccttgaaggt
    15541 aaggttttag catcaggata tacgttgttt gaatcactct tactgatttt attacttaca
    15601 acgatccttt cttttccagt tctcgcattt tctgcatgga agaatgaatt ggcagtctat
    15661 caatttttcg gccaatttga aaaacgaatc tattctacgc aaaaaatcgc tattgtgaat
    15721 cagatccaaa caggctttta ttggaatagt gaagagaatc aaatcatttt tcatgttccg
    15781 aatccagata aaataaattg ggaagtatta gatattcctg atgagattac cttgaaacgc
    15841 catgcttcta ttacttttgc tgctagaacc gggaatgaaa gttcactaaa agcttatcaa
    15901 ttttattggg aagaaaaaga gcggacgatt acgtatcaat tccagatagg gagtggtcgc
    15961 tatacaaaaa gaattgaata aaacaggatt tgttttgttg gagtccctta cagcgcttat
    16021 gatcgcttct gtctgtgtct acttagtgag ctttggccag ctgtttttgc ttcaggcaga
    16081 aaagaaaagt ggggaggaac tgctatttat tcgaatgatt tatgaagata tcaagacgca
    16141 gcggatgtat cacaaaagac cagagcgaag ggaagaaatg atcccatacg atatccaaat
    16201 tgtcgataat aatggagtaa tcgaaaaagt gtttgtcaat atggagaaag aagcgatcag
    16261 tattgaaaaa acaagtaatt ccagcattta cgctgattga atgtctaatt ggactgatga
    16321 tattgagcag tctattgctc acattcaatc ttttgattca gcaaaccatc caagtaagtg
    16381 aatttttgca gcgtaaagat caaaaagagt ggctaatatt tttagcacaa ttagaagaag
    16441 aactcaagag cagtcacaat gtctctgtga aaaatcagca actgcactac acgatcgagg
    16501 ataaacagta tatcatcgaa agatatcagg caatgatacg taaaagaaga acttccggcg
    16561 gacatcaacc tatgttaaca agtatcactg agcttcaatt attggaaaaa gaacacacaa
    16621 tttcttttta tgtacacttt gaaaatggag aagatggata tgccatatgg acaaaaaacg
    16681 atcagtagaa aatgttataa gggctctatt ttattttctg tcttgctttt atttctttta
    16741 tgtacgtttt tattgatggc agcattagaa agctaccgtt tgtcaatgga tcttaatata
    16801 cgaatcaaaa attattatat agcaaaaata atgaagcaga tggctttatc ggaacagtct
    16861 acgcttgccg aaagttcgga aggagttttt tactacacaa ctggatctgt aacatatcag
    16921 tgggtacaac agtccctatt cttagaggtg gaagtctcac cgtttatctt tcggttcaca
    16981 gaagaagtaa aaaatgatac agatactagt acagaatgat ggaatgttcg tgaaaacgcc
    17041 ctcttttctg ttataacact gtttttaata aaaaaatgtt tgccgtttca caaataaaaa
    17101 acagtgaaaa cgattgaaat tttgtcaatt gttcgataga attaaacagg tgaaaaaatt
    17161 gttataatat atctagaaat gaggtgcacc atgttaccag aaaaaatgga acaagggttt
    17221 aatcaaaacc tggaagctat tcagctttta cagaatgcat tgggaacttc ctttttagaa
    17281 gcctatgtcg aaaatgcaga aaatttaata gatgattatc aagtacgtgt ggtggatgga
    17341 gtgccgacaa aagagacaac gcaacggatc acagctcttt atgaagagtt gaagaaactt
    17401 tctttcgagc cagaagaatg gcgcagactg tctcaattac ttttattgaa gggcagccag
    17461 acagaacatt tgcaggcgaa ccatcaactc actcctgata gtatcggttt tttatttgtt
    17521 tttcttattg aacaactata cacaaataaa aaagcaccgg ttaagattct ggatattgcg
    17581 gcaggaatgg gcaacttgtt attaacagtt cttttaaacc tatcgaatgc ggggtaccaa
    17641 acagaaggaa ttggtgtgga tattgatgat acattgttgg ctgtcgcagc ttctacaagt
    17701 gatttgacac aagcaaatgt tcaatacttt catcaagacg ggttgcagga attattaata
    17761 gatcctgtcg attttgcaat cagcgatttg ccaatcggtt attatccgaa tgacgaaaaa
    17821 gcaaaagagt ttttaacaag taccgaagaa ggccatagtt atgcacatca tttattgcta
    17881 gagcaatcaa tgaaatatgt gaagccggat ggttttggtt tgtttttgat gccgagtgga
    17941 tttttagaaa cggatcaaag tgaagaaatc aaaaaatggt ttaaagaaga aggttatctt
    18001 caaggaatga ttcaattgcc agatgaattg ttccgtaata aacaatcgca aaaaagtatt
    18061 ttgatcttac agaaaaaagg accacaagca caacaagtaa aagaagtctt gcttgtgaaa
    18121 cttgcttcat taaaagaacc agagaaggtg acggaatttt tcaacgagtt taaaaactgg
    18181 aaatcatctg ctctgtaatc attcaacgag aaggagagtc atatgtctaa aacaattgca
    18241 atcaatgctg ggagttcaag tttgaaatgg caattatatc aaatgccaga agaaaccgta
    18301 gttgcgaaag ggatcgtcga acgtatcggt ttacaagatt ctatttttac catcaagtac
    18361 ggcgataatc aaaaattcga acaaatcatg gatatcgaaa accacgaagt agcagtaaaa
    18421 atgctgttag atcagttgat tgaactgcaa attttaagct cttatgatga aatcaccgga
    18481 gtagggcacc gtgttgttca cggaggagaa acttatgggg attcagtagt gatcgatgag
    18541 aaagtaatgg accgcattgc tgaattagca gaatttgctc ctttgcataa tcctgcaaac
    18601 ttaatgggga tcaaagcgtt taaaaagatt ttacctgaca ttttgagtgt ggctgtattc
    18661 gatacatctt tccactcaac aatgccaaag caaaattatt tatatagctt accggtgtct
    18721 tattatgaag atttcaaagt aagaaaatat ggcttccatg gcacaagtca tcgttatgtg
    18781 tcggaacgtg cggcaaaatt attgaacaaa ccgatcgaag aactaaaaat catcacttgc
    18841 cacttaggaa atggtgtatc gatcacagca gtcgatggag gtaaatccat ggatacttct
    18901 atgggattca caccattagc tggggtgaca atgggtacac gttcaggtga tatcgatcca
    18961 gctgttctcc catatttaat ggaaaaatta gagattgata ttgatgaaat gatcaatgtt
    19021 ttgaacaaga aatctggtct gctaggccta acaggattgt ctagtgatat gcgtgatctt
    19081 gaaaagaact atgaaaaaga acatattcgt ttagcttatg acattttcgt ggaccgcatc
    19141 cgaaaataca tcggcggtta tgtgacaacg atgaatggtg ttgatgcaat tgtctttact
    19201 gctggtatcg gcgagaatga cggacatgta cgtagtgaaa tcatcaaagg tatgtcttgg
    19261 tttggttgtg aaatcgatcc agcgttgaat gaattgcgtg gtgaagaagt agatatctct
    19321 acaaaagaat caaaagtaag agtattagtg atcccaacag atgaggaact aatgattgcc
    19381 cgtgatgtgg agcgtctgcg cggctaagcc aaagcattcg ttaggataaa agatgaaacg
    19441 catgcaagag ggaattcttg catgtgtttt ttttgcggag aagaccgcaa aatggtaatt
    19501 taacttcaat ttcccttctt ttttacagga tcttcttagg gaattttgct tggaaaagtg
    19561 gtcaaaaggg aaaagactgg cattttattt attgattttc aggaaaatcc caatatttta
    19621 ttttcattca tctttataag ggacattaca tatgtaaaaa gaaaaaacat ttttagatca
    19681 gcctcgtggg ctgtcgactt tattcttcgc tttatgttta gctagaaaac cgatccgaaa
    19741 cattcattga tcgaataata aaaacttgat tttccacctg tactttgcct gaatcgtgaa
    19801 agcacaggta tgaaaatcaa gttttttgtt gcgaaaaaac gtcctagtca atcagttggc
    19861 cgagcggctg gcggctgatc tgctcctgat caataacgag tgcttcttgg aagtgttgct
    19921 gcaaaaagta gcggtcatgg ctgactgcta ggagcgttcc ttgatagtct tgcagcattt
    19981 cttcaatttc ttctcgagca tagctgtcga ggtgattggt tggttcatcc aaaatcagga
    20041 cgttgatttt ttctaaaaag agtttcagca acgccaaacg gattttttca ccaccggata
    20101 aatctttgat ccgtttaccc acatcgcttt gataaaagcc acttttggct aattgccgtc
    20161 gagcgacttc ttcgtttcca gtcaatgtga tggcgtaagc cagcaaacgg ctatcgggat
    20221 cggcaaactg cagctgctgc ggcaaataac cgatttgcaa gctactacct aatttgatgg
    20281 tgccaccgta aggcttttcc tcaccgagta ataatttgag taaggttgat tttcctgcac
    20341 cattgtcgcc gatgatggct aaatgatctc cacgatagac tgcaaaactg ctgtctttga
    20401 atagtacttg atcagcgtac tgctggtgca agttttgaat gatgaaaact tctttgccag
    20461 attttccacc attagacaag ctttgcagcc gatttttcgg gggaggtggc ggtttgacga
    20521 gtgtcagttt cgccaggcgg cgttccagtt ctttggcttt tttgaagaag gactcattgt
    20581 ctccttcatg tgcccactga cgaaaacgac gaatcatgac tttcaaccgc tggacttctt
    20641 tttgctgcag ttcgtaattt tttgtcagct cagccaagcg agcttctttc aagactcgaa
    20701 aacggctgta gtttccggga tactcaatca atgcaccttc ttccagttcc catgtttttg
    20761 tggtgacttg atcgagaaat tgccgatcat gagaaacgac cagaaccgat ttttttgtat
    20821 tctgtaaata gttttccaac cagcgaatac tgttaagatc taaatgattg gtcggttcat
    20881 cgagtaaaag taaatcggca tcggctgcta aaattttggc taaagcgact ttaactcgtt
    20941 cgcctccgct aagttctgtt aaccgtgctt cctgacggtg tggaatccct aagccttgca
    21001 agatcgctgc aatccggtct tctaatacgt aaccgcctag ttcttcaaag cgatgctgca
    21061 gctcaccgta acgggccaga cacttctcta agtttgtatc tggttctgtc atcaatgctt
    21121 cgtagcgact catttgctgt ttgaggtcct gtatctcagc ggcagaatgg agcaagtagt
    21181 ggttgacggt cgcttcggaa actgtaagtt cttgttcgac cgtcccgatc tttaagcctt
    21241 ttttgcgact gataacaccg ctgtcgacac cttctctgcc ggtcaagatt tgtaaaaagg
    21301 ttgatttgcc agttccgttg ataccgacta aggcaatttt ttcaccagct tcaatttgac
    21361 aggataaatc ctcaaataat ggattcccgc cgattgtttt tttaatagaa attgcttgta
    21421 tgatcatgat ctctctcctt gagaccactc aaaaaggcca tggttatccc tggcctgtgt
    21481 tatttaaact cagattatca tgagaaaaat accttgagat ggccattgat tttacgtgaa
    21541 ttcaactgtt ttggacaaac ttgtcccgtc aacagcaact gtaaaaccaa ttgcatggct
    21601 aaatgcgata tggtatcgtt ggtaattttc attacttgtt attttagtca tgcgcatcat
    21661 agtattttcc ctccttcgtg tagttgtctt ttccagtgta cctgaaagtg tcagaaagtt
    21721 caactagcgc tgacatttac ttaatattta tcgatgtgta attctgtcag agagcttttt
    21781 ttatctgact gagggtaatg tctcgtggac cacatcattg tattctgagc taaaccagcg
    21841 ttcaggcgaa atcatatggt gtttgataaa aaactttgtc tacatgactt ccttttgcca
    21901 tttgcttaat accgttgcat taaacagctc ttctctttgg aaatctcacc aacatcgcgt
    21961 tcaggagtat cgttgtggtt gcaatggcaa gcggtagcct ttcatcaatt cttgaatgat
    22021 ttaccgaatg tagcacttgc cttctcgcgg tacccgaacc cgaaataaaa ttttagtttg
    22081 gcgataaaat cggtatccgc catcgatact tcatattttt tcgtatcaga ttcatcggaa
    22141 acttccacat tgacgaacca ataagctttg gcatgcttag gtcgttttaa accacaccag
    22201 ggcgactaat cgccctggtg ttcacttttt ttattttact tgaactcatt gactgttttc
    22261 cctttatttt aaacattata aagaccgatg agtgcttgcc gcgtcttttg tttttatcac
    22321 ggttttttgt gcaaaaaaac ctgccgatag ttggcaggtc gtctacgtgt taagctttgg
    22381 tgtggtcttt agctggcaat tggttttcta aatcagttcg gacaacaagc acatcgcaag
    22441 ctgcgtttct aataacatat tcagaaacag aaccaataaa taaacgttct acagcattca
    22501 atcctgtggc tcctaacata atcaaatcta cttgattatc ttctgggatt tctctggcaa
    22561 taatttgttt tggtgagcca tattctacga cagaggttac attattcaag ccagctttct
    22621 tcgcattgct ttcataatct gccaatgttt gctttgccat ttcagtagct tgttctgcaa
    22681 gcattccatc aaaggatgaa acggtttgga aagaacgtgt gtcaatgaca tgtgctaata
    22741 gaagttctcc gttatttcgg atagctacat tgaccgcctt tttaaaagct aattcagctt
    22801 catcagaacc atctacggca accatgattt ttttgtattg ttgtaacata agcattcact
    22861 cctcaagtca atcgtattgt actcttttat tgtagaacac tttagggaaa aagagaatta
    22921 tttactgctt tggttcagca attttttttg caaatcggat actgaaggtg gcagaaacaa
    22981 taattacgat cagtaaaaag accaaggcaa taaagcgttg attgaaaaaa gctgttaaaa
    23041 tacccattac gcctaaaaca gcatatgcag caccgtaaaa ttgtaggaat ccttgatttt
    23101 tatcattttt gttgatcaat acaaaaaaga tatcctgttt attaaataaa taatagctaa
    23161 cgaataataa tactaaagtg gcgataatca ttaaaatcct aatcatgaac aaatcctcca
    23221 aaataagcaa caaatagaaa aagcaatttg ccaagagcaa attgctttat tctttaaaag
    23281 atggtaatct ccgttaatgc cgttgtttgt cctgtagtat tgatcccgcg aattcaagca
    23341 ggtgggtccc atggtcgtag cttcgaacta ccaaatgata aggcatgtta ccagcagtga
    23401 cacaagacgg gtcccaagat atcaataaaa atgttcggtc aacacacaaa gaggacaatt
    23461 cgaacatcac agatttccca cctctatagt agcatatgaa tttaaaaaga gcaatgagag
    23521 agctcgcaga aacagaaccg ttttcttgtc tagttttttg gaggatgctt tttccactct
    23581 tttatacggt aatactgttg acccagagcc tgttcatatt tccctgtatc ttttggctga
    23641 taatactgag cattcttcag tttatctggt aaatattgct gattgaccca agcctgatca
    23701 aaatgatgag gatactgata acccacgcca cggttgagtg atttcgcacc tttgtaatgg
    23761 ctatctctca aatgatcagg aacatctcca gctttgccct ctcgaatatc agcaagtgct
    23821 gcatctaatg ccatgtaggc agaattggat tttggcgaca agcaaagatc gacgacgacg
    23881 tctgctagtg ggatacgtgc ctcaggcaat cccaattttt cagcagctaa tacagcgttc
    23941 actgtacgag ccgcagcagc aggattacct aaacctatat cttcataacc gataaccatc
    24001 agacgccggc aaatacttgc cagatcacct gcctcaacta aacgggcgag ataatgaagt
    24061 gcagcatcca catcacttcc acgaatcgat ttttgaaaag ccgaaataac gtcataatga
    24121 gcatctccat ttttgtcatg ggtaagagct tttctttgga cacattcttc aatgatcgac
    24181 agtgtaagat gaatccttcc ttctttatct gaaggagtcg atagtgtagc cagttccagc
    24241 ccattcaatg cactccgcaa atcgccgttc gttgcacgcg aaaggtgaag aagagcatct
    24301 tcgtctaact ggattgcttg ttgtccaagg ccgcgttctt tatcccttaa agcatgttcg
    24361 actgccaact ggatgtcttg ttctgttaat ggtttgactt caaagatctg tgtacggctg
    24421 cggatcgctg gattgatggt aatataagga ttctcagtag tcgctccgat caagatgatt
    24481 cggccacttt ctaaatgagg aagcagaaaa tcctgttttg ttttatctaa tcggtgtact
    24541 tcatccaata agaggatcac tgtaccactc atttttgctt cttccgctac aacttgtaga
    24601 tccttttttg aatctgtagc ggcgtttagc atgcgaaatg cataattagt agagccggcg
    24661 atcgcacttg cgatactcgt ttttccagta ccaggaggtc catatagaat catggaagag
    24721 agcatgcgag catcgaccat gcggcgtatg atttttcctt ccccgactag atgctgctga
    24781 ccaacgactt catcgatcgt tcgaggtctc atgcgataag ccaatggctg ttgcatagga
    24841 ttttcctcct tcttgttttt acgtccctta attatatcat atttagcaag atgcctagaa
    24901 agaacagcct tctgttgttt tgttttcata gcttatccaa ctaatattgt gtatgataaa
    24961 gtaggtgaga aaaatgacgg ataaacaaaa gatagatgaa tttttttgga ataaaacgac
    25021 gccagaagtg gcacgggatt tattggggat gtatttggag catgacacac cagaaggaag
    25081 attagctgga tatattgtag acgcggaagc atacctaggt ccagaagacg aggccgccca
    25141 tagctatggt ttaagaagaa caccgcgtgt gcgggcaatg tatgaaaaac caggtacgat
    25201 ctacctttac acgatgcata ctcatcggat tttgaacatc attactcagc cagaagggat
    25261 tcctcaagga gtaatgatac gtgcaatcga acccgccgca atgattgatc aaatgagtaa
    25321 aaatcgtggc ggaaaaactg gtccagatat cagcaacggt ccgggaaaat tagtggaagc
    25381 attagcaatt cctcaagaac tttatggtca gtctatagca gatagttcgt tacgcttagt
    25441 ttttgagaaa aaaaagacac caaaaaagat caatgctttg cctagaatcg gtatcccaaa
    25501 caaaggtgta tggacagaaa agccgttgcg ttttgttgtg tcgggaaatc cttatatctc
    25561 tttacaaaga aaaaaccaaa tcgagaaaga ttggggttgg aggaaagaaa atgaaaaaga
    25621 aggatctatt aacatattta gatgaaataa ttgaaaaaca agtgacagac tatgatgtag
    25681 ctttagattg ggatacaaaa aatcatacga tcgagatcgt tgttcgcttg tttgcagaaa
    25741 ataaagcaca atttgagatt gatgatgcag aagggatcgt ttcgcaagaa ccaattatcg
    25801 aatttgaaga cggcgtactt ctttttaatc ctcagaaatc tgtttttgat gaacgcaatt
    25861 atctagcagt aatcccttat gaaggaaaaa aagggttggc aaaatcagta gctgattctt
    25921 tagtggaata tctaaatgaa gtattagctc aagggcaaag cgatttgctt gattttttgg
    25981 atgaagacga tgacgaagct gtattcgagc ttcactggga taatcaacaa ctagcagaat
    26041 tagtagaagt aaaacagcaa aatgaagcag atacttattt accgtaccca agttattaag
    26101 gagtgacaga tatgaaatgg aatgaagtaa aagtagaaac tgaaagtgaa gcagtcgaag
    26161 cagttgctaa tattttgatg gaggcagggg cgagtggtgt cgcgattgaa gattctttag
    26221 atgttgaaca ttttcagtcg gatcaatatg gtgagatttt agataaaaat gattttacat
    26281 cgctgaaaga aggcgcacta gtcatggctt attttccgga aacgactttc ttaccggaaa
    26341 tcttgccttt tatcaaagat cgtatcacac aattacctga gtttggtcta gctattggaa
    26401 aaaataaagt aacagttagt gaagtagagg aaagcaactg ggcaacagcc tggaagaaat
    26461 actatcatcc ggtgcgtatt acgcgaatgc tgacggttgc gccaagctgg gaaaactacc
    26521 agacagaaga tcctctagaa aaaattatca cactagatcc tggtatggca tttggcacag
    26581 gcactcatcc cacgacttcc ttgacattga cagcactaga aacagtcttg cgtggtggtg
    26641 aaacattgct agatgtggga actgggtcgg gcgtgctaag tattgcagca aaatatcttg
    26701 gtgcaaaaga tgtctatgcc tttgatttag acgaagtagc cgttcgttcg gcgaaagaaa
    26761 atatggatat gaatgaaata gccaaagatg tccatgtatc agctaacgat cttttgacag
    26821 gaatcgagat tgaaagtgat gtaatcgttg cgaacatttt agcagatatc atcttattga
    26881 tgatcccaga tgcttggcgt ttactgaagc aaacaggcac gctgatcgtt tcaggaatca
    26941 ttgaagagaa gaagcaaatg gttctggatg ccatgttaga acaagggttt atcgtggatc
    27001 aaattttcca gcaaaaagac tggtatgcaa ttattttgaa aaaaccagag gtggaataga
    27061 tgcagcggta ttttttaaaa gaatcatatg aatcaaaaga ctattttgtt ttagaagggg
    27121 aaaactatca ccacattgta cgggttatga ggatggaacc cgaggatgaa gtttttttgg
    27181 cattcaatga tcaaatagcc attgttgcag aaatcacgga gattactgaa gaggctgttt
    27241 atctgaaaga aattaaaaaa gaagaaaatc aaaaggaact gccaagttcg attaccatag
    27301 cgagtggcta tcctaaaggg gaaaagctgg attggatcgt tcagaaatcc actgaattag
    27361 gagcgcaagc ttttcttggt ttcccggcat cagcctctgt cgttaaatgg gatgcaaaaa
    27421 aacgtatgaa acgacaacaa cggcttgaaa agattgctca agaggcagcg gaacaatccc
    27481 atcggcagat cgtgccatca gtagagttat tggaaacgaa acaagattta tttgagcggc
    27541 tagatgatta tgacgtggtc ttggttgctt atgaggaatc agcaaagcaa ggcgaaaaat
    27601 caaatgcagc caaaattctt agtgaaacaa aaccagatag tcgaatctta gctgtctttg
    27661 gaccagaagg tggattatcc cgcgatgaag tcgattattt tgtagaacac aaagggatac
    27721 tttgtggatt aggaccaaga atccttcgta cagaaacagc acctctttat ttattaagtg
    27781 ctgctagcta tcaatgggaa ttaatgaatc cttctttcag ctaacgttct tcattaataa
    27841 aagagtatcg acagaaagaa ttgaaaaaat agtcaacaat ttgtataata gaaacaatta
    27901 tttatacaat ggatacgaaa aatcgtttga aaaagctttt aaaaagcgaa catttccgta
    27961 aaaaaacgta taactgcacg gattttggga caattatcaa acagatattt gtcccgattc
    28021 ccgttttacc gaaaaaagaa agggggatcg attcctgtga caaaagaaga aattttaacg
    28081 ggacccggtg tcataaagct cgttagtcaa tacatgggtc ccgaacatgt agcatttgtt
    28141 gagaaagcat gcgaatacgc gactgctgca catgacggac agtttagaaa atcaggcgaa
    28201 ccctacatta ttcatcctat ccaagtcgca ggtatattag cagatttaaa aatggatccc
    28261 catacagtgg ctacaggctt cttacatgat gttgttgaag atacagaaat cactttagaa
    28321 gatctgagag aagaatttgg cgatgacgtt gctatgttag tggacggcgt aaccaaatta
    28381 gggaaaataa aatataaatc ccacgaagag cagctagcag aaaaccaccg aaagatgctg
    28441 ctagcaatgg ctcaagattt acgagttatc atggtcaaat tagctgatag actacataac
    28501 atgcgtacgt tgaagcacct gcgagaagat aagcaaagaa ggatcgctca ggaaacttta
    28561 gaaatctatg cgcctcttgc acatcgtcta gggatcagcc ggatcaaatg ggaattagaa
    28621 gatacagcac ttcgttatct aaatccaaaa cagtattacc gtatcgtcca cttgatgcag
    28681 acgaagagag aagaacgtga aaaatacgta agtggtactg ttgaagatat tcgaatagcg
    28741 acagaagagc tggggatttt tgcagaaatc tatggacggc caaaacacat ttattcaatc
    28801 tatcgtaaaa tgaaagatca gaaaaagcag ttcaacgaaa tttatgactt gctagctatt
    28861 cgagtgatcg tagattcaat caaggattgt tatgctgtat taggagcaat ccacacaaaa
    28921 tggaaaccaa tgcccggcag atttaaggat tatattgcca tgccaaaagc taatatgtac
    28981 caatctttgc atactaccgt gatcggtccg gcaggaaatc cggtagaaat tcaaatccga
    29041 acacaggaaa tgcatgaaat cgctgaattc ggggttgctg cacactgggc ctataaagaa
    29101 ggaaaaaatg aaaaagtaga accagatggt atgacgaaac aattaagctg gttccatgag
    29161 atactcgaac ttcaagacga aagctatgat gcttctgaat ttatggaagg cgtaaaagga
    29221 gatatcttta gtgataaagt ctacgtcttc acaccaaaag gagacgttac tgagttacca
    29281 aaaggatccg gaccattgga ctttgcatac agtatccata cagatatcgg taacaaaacc
    29341 actggtgcaa aagtaaatgg caaaatggtg cagcttgatt acaaattgaa aaacggagat
    29401 atcattgaga tcatgacttc tccaaattca tttggcccaa gtcgcgactg gttgaaatta
    29461 gttgctacta gcaaagcaag aaataagatc aaacgtttct tcaaagccca agatcgagaa
    29521 gaaaatgtga tcaaaggcca cgaatccgtg gtcaaatgta ttacagatct aggatttacg
    29581 cctaaagata ttttgacgaa gaacaaactg caagaagcac tcgatcgttt taattatcaa
    29641 acagaagatg atctctatgc agctgtaggg tatggagaag ttagcccctt gacgatggcc
    29701 aatcgtctga ctgaaaaaga acgtaaagaa caaaaaatcg agcagcaaaa gcaagaagca
    29761 gaagaaatca tgaatcagcc gaaaaaagaa cctgacaaaa tgaaagtacg tcatgaaggt
    29821 ggcgttgtca ttcaaggggt agaaaactta ctaattcgta tcagtcgctg ctgtaatccg
    29881 attcctggtg atgatatcgt tggttatatc actaaaggca gagggatatc cattcatcgt
    29941 cgagattgtc cgaatgttca gcctgacaaa ccaaatgtag cagaacgttt gattgaagtc
    30001 gaatgggaag atacatcgaa tacacgaaaa gagtatgatg cagatttgga aatttacggc
    30061 tataatcgtt caggcttatt gaatgatgta cttcaaacag tcaatgcgct aacgaaaaat
    30121 ctcaacagcg ttgaagcacg gacgaataaa gataaaatgg cgacgatcca tttgacggtt
    30181 ggtatccaga atttatccca cctaaagagt atcgtggata aaatcaaagc agtacctgat
    30241 gtctacagtg tacgccggac gaatggatag gaggacagat atgagagcag ttgttcaacg
    30301 ggtaagtaaa gctgctgtgt cagtcgatca gcaagaaatt ggaaaaattg gctacggcct
    30361 acttgtttta ttaggcattc atgaaaccga tacgcaaaaa gatgtggact atctggtaaa
    30421 aaaaattgca caaatgcgta tttttgaaga tgaacaagga aaaatgaata ttagtgtaga
    30481 agatgtggga ggacaaattt taagtatctc tcaattcaca ctgtttgcgg atacgaaaaa
    30541 aggaaatcga ccaagctttg tggaagccgc acgaccagaa acagctattc ctttatacga
    30601 atcattcaat gaaggaatca gaagtcgcgg catcacagtc gctaccggac aattcggagc
    30661 agatatgtcg gtttctctcg tcaatgatgg acctgtaacg atcattattg attctaaaaa
    30721 caaataaagt aatctggttt tcactagtaa aaactatgaa acacgtcgct taagttagac
    30781 tgataaactc taacttaagt ggcttttttg tctgcaaaaa gcgcttcttt tgtcagcagt
    30841 cggaaaaaaa gttaagctta aattatacaa gttatttttt ataggaggtt gctgaagatg
    30901 aaagaagtac cagtagtaga tatcaaaatc acagatttaa aaaaattgta tcaaaaagga
    30961 tacaacatgt tggaggagtt acgccatgaa gcggacgctc ccgtcgtcaa agcaaagatt
    31021 ttcaataaag aagctatcac gatctacgga tcatcagcag caaaagtatt ttatgatcca
    31081 aggaatttca aacgaaaagg tgctatgcca aaattagtat tgaagacact ttttggtcaa
    31141 ggtggagtac agactttgga tggagctgct catcaccatc ggaaaaatat ttttatggat
    31201 ctaatgacac cggaaagaat ggaagattat catcgtatct tagataagaa tctgacacaa
    31261 gcacttgaag cacagcatgg tcaatttgaa ctatttgatt taagtaagat ggttttcttt
    31321 acatctattt gcgagtgggc aggtatcaat ttatcagcga tttctaaaga tgaggtcgaa
    31381 aaattagcag aatatcaaat ctctatgatc agcggaacgt ttacttctcc gattgatcac
    31441 ataaaaggtg tcgagaatcg caaaaaatca gaaaaatggg cgcaaggctt gattgaagaa
    31501 gctcgacaaa atcctgtggc tggaaaagaa aatgtggcat tatatgcttt tgccaatgcc
    31561 acagatttag atggtcagct tttacctctg gaagtggcag ctgttgaatt attgaacatc
    31621 atccgtccaa cggttgctct aactgtgtgg gcggcattga tgggacatgc acttttcagt
    31681 agaccagatt tgtatcagca attaaaaaat gacttttcga cgttacaaga tccgtttatt
    31741 caagaaatga gacgctatta tcctttcttc ccaatgctac ctgcgatttc gctgaaagaa
    31801 gtagaagtag atggttatcg tataccagaa ggcagttggg ttattttaga tttatatgga
    31861 acagaccatg atgaacgaac agttgaagcg ccagattcct ttatgatcaa acggtatgta
    31921 gggaaagcaa aagatatttc ctataaagaa gaatatgaga tgattgcaca aggcggggga
    31981 aattttcgac aaatgcatcg ctgtgcagga gaatggatca ctcttcatag tctacgtgtg
    32041 ttttccgatc agctagtaaa taagtttgag ttctctgtac ctgaacaaga ttggacgatt
    32101 ccattcaatc agtttccgac ttatccgaat agccgagcgc tactttacaa gaattagatt
    32161 agcataaact tatcgaaaaa aaggaagatc aaacagtgct gatcttcctt ttttcatatt
    32221 actataaatt gataaggtca attgatcaaa atccacgaaa tgcttcatca atggtttgat
    32281 aatcttcatt ggataaatgc aaatctaaag cttggacatt gtcagatact tgctctggac
    32341 gcttagctcc aggtataact acagacactc gtggattctt catgtaccaa gctaaaacaa
    32401 gttgcgcagg agtggcttgg tatcttttag caatggggcg aagtacatcg acagcggtta
    32461 aaatttctct gaaacgttct ccttggaaat ctggatttct ttttcgagga tctccttcac
    32521 caaattgttt ttcctcacca agttcatatt ttcctgtcag caaaccggaa gcaagaggaa
    32581 aatagggaac aaaactgatt ttgttttttt caagataagg gaataattct ttttcagcct
    32641 gccgatggat caaactgtat ttatcttcta ctacatcgac atatccatct gcattcgctt
    32701 ctttcaattg ttctaacgtg aagtttgaca caccgactgc tcggattttt cctgcttctt
    32761 ttaattcgtg caatgtagca actgattcat tttttggtgt gctttcatcc ggaaagtgga
    32821 tataaaaaat gtcgatgtaa tcagtttgca gacgttttag tgcatcttcc accgattgtt
    32881 tgagaaactc aggagaatta tcaaatgttc gccctttatt tggtacatga gcagcttttg
    32941 tagcgatgac gacacgtgag cgatcatatt ctttttcctt cagtacctcg ccaatcagtt
    33001 cttcggaacg gccgtttccg taagcataag cggtatcgat caaattgatc ccgctattca
    33061 aagctgtacg aactacttgt tttcctgtct catcttccaa accgtcaaaa agattatgtc
    33121 ctccaacggc atttgctcct aatccaagct gttctgcgta gacttgtgaa tgtccgattc
    33181 ttatttctgc catagctgat tcctcctttt tgtctagtct aaaacaaaat accaaaaagc
    33241 tgaactaaaa cgtttttgtg aggggaaata gtcagatatc tcttgacttt gagactggtt
    33301 tttagtaggt tataagtaat atcgaatgtc aatgaaagag cgagtaagtt tttgaaactt
    33361 tacaagagag gatcatcgag gctgaaaatg atctaaagtt tcattagcct aaagacactc
    33421 tggagatgga cgagtgaaaa gccgtcccgc gtacgagcgt tattcgtaag aggaaagaga
    33481 aatctttcaa aattaggtgg taccgtgtat gaatttcatt cacccttgtt catgatgaac
    33541 aagggtgatt tttttattta catagctaag aaagggagag aaaactatga gtttccaaag
    33601 accaaaagga acaaatgata ttttgccagg cgaatcagaa aaatggcagt ttgtcgaaga
    33661 aacagcacgt ttattattca gagattatca gtataatgaa atgcgtacac cgatttttga
    33721 acactatgaa gtgatatccc gaagtgtagg ggatacaacg gatatcgttt caaaagagat
    33781 gtatgatttt tatgacaaag gggaacgcca tgtgacgctt cgaccagaag gtacagcacc
    33841 aatcgttcgt tcttttgttg agcataaatt gttcggccca gagtatgcga aaccgttcaa
    33901 aactttttat atgggaccaa tgtttcgcta cgaacgccct caaaaaggac ggttacgtca
    33961 attccatcaa atcggagtag aagcatttgg atcagaaaac cctgcaactg atgtggagag
    34021 catggtgatg gcacttgatt tcttcaaaca gttggggatc acgcaaatcc ggctagtcat
    34081 caattcatta ggtgataaag aaacgcgtca agcctatcgt caagcattga tcgactatct
    34141 tttacctttt gaatcagagc tgagtgaaga ctctaaacgc cgccttcatg aaaatccatt
    34201 acgcgtattg gacagtaaag acaaacgaga tcaaaaattt gtggcagaag ctccatctat
    34261 cttagattat ctaagcgagc ctgctcaaaa acattttgat accgttgttt ccatgctgga
    34321 tgcattggct gttccttatg aaatcgacag taatatggtt cgcggactag attattacac
    34381 acatacgatt tttgaaatca tgagtgacgc accaaaaatg ggggcacaag caacgatttg
    34441 cgcaggcgga cgttatgaca atctagtgga agaactaggt ggtccttcaa cacctggctt
    34501 cggatttgca atgggtatcg agcgattact cttgactatg gaggcagagg aagtagttat
    34561 ccctgcattt aatgagttgg atgcttacgt ggtcgcactc ggagacgaaa caaatatcga
    34621 agcattgaaa gtggtccaag cgattcgaaa ctttggtttt tcagctgacc gcgacttcat
    34681 gaaccgtaaa gcaaaagcgc aatttaagac agcagataaa gcgaatgcca aattagtatt
    34741 ggtgatcggt gaagatgagc ttgctagtca aacgattaat gtcaaagcaa tggcaagccg
    34801 aaaagaaaaa agttttccat tagaagagat atatgaacat tttgatgaag tctatgatga
    34861 aatgacttta ttcgagtagg aggaaaaaaa taaatggcac aaagaacgat ctattgcgga
    34921 ttagtttccg aacaacaatt agaacaaaca gttacattaa aaggatgggt acaaaaaaga
    34981 cgagaccttg ggggagtaat ctttatcgac ctacgtgatc gtgaaggaat cgttcaaatc
    35041 gtctttaatc ctgaaatcaa caaagaagca tgggaaatcg cggataaatg tcgcagtgaa
    35101 tacgtgatcg aagtgactgg ggaagttaga agacgcggag aaaatgcagt aaatcctaaa
    35161 atggccactg gtgaattcga agtgattgca agtgaaatcg tgattttgaa tcaagcaaaa
    35221 accccgccat ttcaaattga agatgatcaa gcaatcagtg acgaaatacg tttgaaatat
    35281 cgttatttgg atctgcgtcg tccaaaaatg actaaaaaca tcaaattaag aaatgcgaca
    35341 acaaaagcga ttcgtcatta tttagatgat aatgactttc tcgatatcga aacaccttac
    35401 ctagggaaat ctacaccaga aggcgcaaga gactacctag ttccttcacg tgtccatgct
    35461 ggacatttct atgcgttgcc gcaatctcct caattgttta aacaattgct gatgaatgct
    35521 ggattcgacc gttattatca aatcgttcgt tgtttccgcg atgaagattt acgtggagac
    35581 cgtcagcctg aatttacgca agtagatatt gaaacaacgt tcttaacagc agaagaaatc
    35641 caaacttaca cagagggatt gattgcgaag gtaatgaaag aagttcgcgg tatcgaagtc
    35701 acattgccat ttcctcgtat gacctatgat gaagcaatgg ctcgctacgg cagcgacaaa
    35761 ccggatacac gttttgatat ggaattgatc gatctaagcg atacagtcaa agaagtagaa
    35821 ttcaaagtct tccaaatggc tcttgaaaac ggcggtgtcg tgaaagcatt gaatgctaaa
    35881 ggcgctgcag atcgctactc acgaaaagac atggatcagt taggtcaata tgtaggacaa
    35941 ttcggagcta aggggcttgc atggctgaaa gtagaagaag acggattgaa aggtccaatt
    36001 gctaaattca tgggagaagc aacagaagca atcattaaag caacagatgc aaaaccaggt
    36061 gacttgctga tgtttggcgc agataaaagt gaaatcgtcg ctgctgcatt aggtgctatc
    36121 cggacacgtt taggaaaaga attaggacta attgatgaat cgaaattcaa tttcttatgg
    36181 gtaactgatt ggccacaatt tgaattttca gaagaagaag gtcgatatgt ctctgctcac
    36241 catccattca cgatgccaaa agaagaagat atcccgttat tggctgaaga ccctgcaaaa
    36301 gtctatgcag aagcttatga tatcgtcttg aacggttatg aattaggcgg aggttctatc
    36361 cgtattcata ctcgtgagct tcaagaaaaa gtactagaaa ctctaggctt tacaaaagaa
    36421 caaatggaag aacaatttgg tttcttgtta acagctttag attacggatt ccctccacat
    36481 ggcggtattg cattaggatt ggaccgtttt gcaatgttat tagccggaga agataatatt
    36541 cgtgaagtca ttgcctttcc taaaaacgga aaagcagcag atgtgatgag tgatgcacca
    36601 agtatggttt cgcctttaca attgttcgaa ttgaatattg atgtaacgaa tatagaagaa
    36661 tagccgataa aaaggtaaaa aggatttgtc gaatacgctt cttttgacaa ccttgattta
    36721 attgaaaaac actttataat gaagccataa catataaagc ggggcttttc catgaaaaaa
    36781 acattcgaaa attgggctta tcatgattac acgaccaagc tttcggtatc tatcgtttat
    36841 gcgttgatcg catcagtcgc attgaatttc ttttatcagc caggacatat ttattcaagt
    36901 ggtattactg gtctagctca gattttgacg actttgtcaa aatcactgat aggattcgaa
    36961 gtaccggtat caacaacttt gtatttactg aatatacctt tgtttgtatt ggcgtggtta
    37021 aaaatcggga agaagtttac aattttcact tttatcacag tcactttgac ctccctttgt
    37081 atccatattt taccggaaac agtcctatct accgatccaa tcatctgtgc gttgtttggg
    37141 ggggctgtga tgggactagg tattgggcta gcgttgagaa acggcctatc atcgggcggt
    37201 ctagatatcg tgagtatcac gattcgtaaa aaaacaggaa agtcgattgg ttctatttcg
    37261 atctacttca atgcattgat cgtttttgtt gccggaattc tatttggctg gcaatatatg
    37321 ttttacagta cgttgtctat tttcgtcagt ggaaaagtaa ctgatgtcgt ttatactaaa
    37381 caaaagaaga tgcaggtaat gatcgtaacg aaacatccag aaaaagtagt aaatgaaatc
    37441 caaaactgta tgcgtagagg gatcacgatc atcaatgaag cagaaggtgc ttataagcat
    37501 gataagcaga cagtattgat gacgatcgtt acacgatacg aactgcctct acttagagaa
    37561 gcaatgaaag cttctgatcc acaagctttt gtaagtattg cagaaaacgt ccagatactt
    37621 ggtcgtttct atgaggatgg catttagatc gagtttgaca aaaattcatg aaatactgct
    37681 gtagacagac gttcgtttgt ctgcagtttt tttctaccat agtagaatca gcaaagaaaa
    37741 actgcttttt tatttgaaat tttcttaaag ttgaaaaaaa gcatgtttat ttttttaaat
    37801 ttatgggatg atagaaaaaa atagtagaga aaggacacga ttctacttgc tattttcact
    37861 taatacccgt atattacgaa tgaaaaaagt ttatgaaaag ttggaatatt gaatggaaaa
    37921 acaaaaaagg ttaaaaaaca gccgctacat cactgggttt gatgggctcc gttcattagc
    37981 tgtggtgggt gttatcttct atcaccttct gccaactagt atgaaaggcg gctatttggg
    38041 cgttccgatt tttttcgtcg tatctggtta tttgatcacg gatctgctgc ggcaggaatg
    38101 ggaacaaaat ggaaaaatca atatttggca attttatgtc agaagaatga agagactcta
    38161 tccggcactt gtttttatgc ttgtgacagc ttcggcttat atcactttgt ttcagcgagg
    38221 gctgttaaac aatcttcgag gagttgtggt cagcagctta ctgtatgtca acaactggtg
    38281 gcaaatcaat aacggtttat cttatttcga tcgatttgct aatgagtcac cgtttacgca
    38341 tatctggtct ttagcagtag aaggtcaaaa ctatttggtt tggccgatct tgtttgtatt
    38401 gttgatggta tttgttcgta aaagaaaatg gatcttttat actgtgttag gtacatcctt
    38461 cctctccgcc atcttgatga tgatcttgta tacaccagga ggcgatccaa cccgtgttta
    38521 ttacggtacc gacactcgtc tgttttctat atggttggga agtgcgcttg cgtttgtttg
    38581 gccaagtacg cgtttaaaga aaaacatccc gaaacaagcg aaaaatattc tgaatatcgc
    38641 tggaggaatc tcgcttttgg cactgattct ttgcttcttc tttttagatg atcattacaa
    38701 tttcatctat tacggaggaa tgctcctagt gagtctgtta tgcgtagtgc tagttgcagt
    38761 gacagcacac cccggagcaa gtttgaatcg atggctgaca aatccagtat ttacttggat
    38821 tggcaaacga agctatggca tttatctgta tcagtttcca gttatgattt tttatgaagc
    38881 aaaggtgact gatattgccg atcatgtttt gttgcatact ttagtggaaa tcacattgat
    38941 tctaatcatc agtgaacttt cttatcgata tgtggagcgt ccgctagcac gttttgatta
    39001 tggtcgtaca ttccaagtga tgaaaggatg gttcagcaaa ccgatcatca gtaagagcaa
    39061 accatgggtt gttccaggca cattacttgt agcaattgca ctcgttggtt tcgttacggc
    39121 accgaaaaac gctgtaacag ccgatcaaaa acaactgcag gcaaaaatcg ctgaaagtaa
    39181 aaaattagca gaagaaacca gaaacaattc agaaaacacc actagttcgg ttgaccaagc
    39241 gatccttgat aagtacgagt tgtccaaggc acaagggaat aaggcacaaa aattagaact
    39301 aacggcattt ggtgattcag ttatgttgga tgctgcaacg gatctacaag aactttatcc
    39361 aaaagtagtg gtagatggag atgtcggacg acaattatat gccagtgtac cgtacattga
    39421 aaaattaaag gaacaagatt tgttgaaaga cactgttctg attggactag gaacgaatgg
    39481 ctcattctcg gaagctcagt ttgatagtgt gatggatgca atcggtgatc gaaaagtata
    39541 ctgggtcaat gtccgagtac cgacgaagcg ctggcaaaat gacgtgaatg cgatgcttga
    39601 acagatgacg aaaaaatacg acaatatgac gctgattgat tggtacagct acagtaatga
    39661 ccatagtgac tggttttatg atgatcaagt ccatccaaat cctgaaggga tgacacatta
    39721 cattcactta gtcagcgaag ccttgctagg aggaagttca agtacgaaca gcagtgagac
    39781 aaacgtgaca gaatgatagc tacaaaaaac ttcaggcaaa aacatttgtt tttgcctgaa
    39841 gtttttttat atttaccgtg tttttaacat tcatatagat tcaggaagca aaacggttga
    39901 gtgtgtctgt aaataacgtt ctttttgttt tcgggtgagt tttttgaatg cagcttctgc
    39961 ttttgttgct tcgcttcttg aagaaaatac ttctgcatgg atcatcttaa caggtcttct
    40021 tttttgaaga cgagtatatt tagcaccagt accattgtta tgttcctcta atcgtctaga
    40081 aagatcggtt gtatatccac cataaaatgt ctgatcttta cagaataata cgtaaaaata
    40141 atgttcattt gccatagagc atctcacgaa tctctgcagt gtattctcca tccatattgt
    40201 aagtgatcaa tggtggagcg actttgaatc cgtctaattt gccttctttg attccttcaa
    40261 ctaacaaaat atttgcctct tttccaggtt ttgggtaaac aaattggatc cttttaggtg
    40321 caattcgata gtcttccatg acctgaatga tttctaagaa ccgatcagga cgatggacca
    40381 tagtaaagcg tccatttgtt tttagtagct tacttgaaat atcgatcact tcttctaacg
    40441 tcgtatgaat ctcatgacgg gcgattgcca ggtgtttatt agggttcgtt ttatttgttg
    40501 gcagcccttt gaaataaggt ggattacaga cgactaagtc acaagaatta tgtcgaacga
    40561 cagataagct gtctttcaga tcgatggtgt ggacagtcat ttgttcattt aaatggttaa
    40621 gcgcgatact acgtcttgcc atgtctgcta gacgaggttg aagttcgata gcgtcgatag
    40681 ccgcctgggt tcgtttactt aaaaaaagtc caactgcgcc atttcctgca caaaggtcaa
    40741 caatcttccc ttttttaggt acacgggaaa agtgagcaag cagcacagca tctaaagaaa
    40801 aagaaaacac tttggaactt tggataatct tgatatcttc tgcgtataat tggtcgattc
    40861 gttcgtcttc gtgaagcatg gattccctcc aaaatgaaaa aaatagtcgt ctgtattata
    40921 ctatttttat taaaaagaac caaggtggct tgtcaaacct ctatttttta tgcatactat
    40981 gaccataaga agggaaagga agagactatg ttttatcgat ttatgcgcgg ggtagttcga
    41041 gtactattag ccttagttaa tggaaatgcc cgctatgaga ataaagaagt tttaccgcaa
    41101 gatgagaatt acatactagt tgctccccat cgtacttggt gggacccatt atatctggcg
    41161 gtagctgcag cacccaagga attcagcttt atggcaaaag aagaactgtt caaaaatcca
    41221 gttctgcgat ttatattaac tcgatccaat gcttttccag ttaaaagaga aaatccagga
    41281 ccaagcgtca ttaaaacacc tgtaaagatt ttgaaatcga caaatttgag tttgatcatg
    41341 tttcccagtg ggacaagaca tgctacagaa cttaaaggcg gaatggcatt gatttccaga
    41401 atgtctaaag cgaaaatcgt gccagctgta tatcaaggtc cgttgacact tggggatctg
    41461 ttcaagcgtc agagagtgac agtgcgtttc ggcgaaccaa tcgacttatc agatatcaag
    41521 aaaacggata aagaaggtat ggcggagatc gaacgacgaa cacaagcagc ttttgacaaa
    41581 ttggacaaag aagtgaaccc tgatttcaaa tatgaagtca aaaaaaaata aaatgataag
    41641 tttatgcgtg ggcggtcttt tgaatctact gtcctgttca gttcagactt tttcaatgaa
    41701 aacgagggag agacgtcaag tctgccctct ttttgcgttc caatatgtaa aatagaagac
    41761 aggtgtcatg taatggatca aagtgttata ataattagaa ggacattttt tgaggtgatt
    41821 gaatgcgttt tttacataca gccgattggc atatcggaaa aaaactacaa ggttatgact
    41881 tgattgaaga ccagcgtcat gtactatctt ctatactaga tatcgcgaaa gaagaaaaag
    41941 tagaggcaat cgtgattgcc ggagacttat atgataggag tgtcccagct gttgatgccg
    42001 tagaattata caatgaattg atggtcgatt ggaatttaaa ggaaaagatc ccgatttttg
    42061 ctatatcagg aaatcacgat agcagtacga gactttcagc gggtacgcca tggttcagtt
    42121 attcgaacta ttttttgcga acacagctag cagaagcatt tgaaccagtc gagtttaaaa
    42181 acgtacaatt tttctttctt ccttactttg aaccaattgc agcgagatta tattttgaag
    42241 atgacaatat ccgaacgatc aagcaagcgg tagccaaggt gatagataaa atgcaggagc
    42301 tatttgattc agaaaaaaaa caagtattga ttagccattt ttttgtaatg ggaagtgtaa
    42361 aatctgattc ggaaacgaaa atcgaagtag gcgggttgga ttcgatccct ggtgaactac
    42421 ttacaccgtt tgattatgta gcactaggac atttgcataa tcaagctgct ttgagacagg
    42481 ataatgctcg ctatagcggt tccccattga aattctcttt atctgagatc aaccaaaaaa
    42541 aaggcgtatg gatcgttgac ctgacaacag gcctggagtt gaattttagg gagatcgaac
    42601 ccctgcatga tattgaagaa ataacagcga gtttcaaaga attgctggat cctgcctttt
    42661 atcagtccgt caatcgggag aattacatct atatctatct cactgataga acgatcatcc
    42721 cgaatatgat gaaccagctt cggcaaatat atcctcgtat tttggggatt gaacgggcaa
    42781 acggtcgagc agaacaaaca aagcgcaaac gaaaagtact agaaatgaaa gatcctcaaa
    42841 aattagcaga agcatttttt agtgaaatga cggaagagca gctaactgca aaccaagaaa
    42901 aatggctcgg agaaacgttg caagaaatca tggaaatgga atgatcaaga atgaaaccga
    42961 aaaaattaca acttaaaaat tttggtcctt ttatcaatga gaccgtagat ttttcacgtt
    43021 tgacagaagc accgcttttt ttgatcagcg ggaagacagg agctggcaaa acgacgattt
    43081 ttgatgggat caccttcgca ctttttggtg aaacttccgg aagacttcgc tctggaaaag
    43141 agatgcgttc tttgtttgct acaccagacg aagaaacaag cgtgactttt tcttttgaac
    43201 atcagcagat gagctatgaa atcgaacgaa aaccagaaca agttttagca aaacgaaaag
    43261 gaaacggaac aaaaaaacag gcagccaaag taacgttgac catttttgat gggaaaggaa
    43321 aagaaataaa acaaatcaca aaacgtacag aagcagatca gttgatcaga gaactgatga
    43381 atctcgatgc gaaacagttt tctcaaatcg ttttactacc ccaaggagaa ttccgtaatt
    43441 ttttggtttc atcaagcagt gataaagaga ctgttttacg tcatttattc ggtactcaat
    43501 ttttccaaca gttcaatgag cgattaaaag aaaaagcaaa acaacagcaa caaaaattgg
    43561 atcatttaga gcaagagctg catcttctgc aaaaaaggtt tgttttagtc aaagaagagg
    43621 aaccagcagc agggtttgaa gccgttttag cccaatggac aaaccatcag gcaatactcg
    43681 aaaaacaaat cacggaagaa aaagaagctc taacggaact tcaggaaaaa caaaagcaac
    43741 tagaaaattc ctactatcgt tttgaaagct tgaaaaaaag ttatgaagaa aaaacgatat
    43801 taataaacaa gcaaaaggaa ttagctgctc aaaaagacga aatagaggaa aagaaacgat
    43861 ggattgggta ttatgaattt acgcaacgat tagtcgaacc aatccagcgc ctacaagaaa
    43921 taaaagttga aaaaatagaa ctgtcacaaa aaagtagtga gcaaacaaag aaactagcga
    43981 tgactacgca gaattatgaa gagtggcaaa aaaatgaagc aaaccgcgta cgccgacaaa
    44041 caaaaatcaa agaagctgct caaaaactgc aaaacaaaca gatgatggtg ccaattgtcg
    44101 aatccttacg tagtaaaaaa caagacgcag aacaattaac aatttcattg gataaagaag
    44161 aattgaatgc cgagaagctg tatcaaaatc tcgctatcta tcaggaaaga actcgaattc
    44221 ttcaagaaaa tttactggat caggaaaaac tgcaagaaga tcgtcttgct tttggacagt
    44281 taaaatatct agatgaaaag cagcaagaaa tagaaaaaca aaaagcaaaa gacactgggt
    44341 cgctcgcaca gataagagaa gaactcaaaa aagcaactga agaaataaca aaaaatagtc
    44401 agctattgaa agaacagaca gaacaagcag aacagctgaa aagccaatgg gcaaagcagc
    44461 aaattgctcg tcttcagctt tttttgcgtc caggggagcc atgtcttgtt tgtggatcaa
    44521 cggaacaccc catacaacaa ttaaaacacg aacagccaaa taaggaagat atcttaaaag
    44581 tagaacaaca actggcagaa gctgagtctg ctgtagaaag aactcagcaa atcattgcca
    44641 aaagtgaagc acatgaaaat aatcttcaac aacaattatc agatttagaa caaaaaatca
    44701 tggcaacgaa tatacaattg gctgaacaag aagaacactt gcaacaggct ttgaaaaagg
    44761 catttcctga gataacagaa caaaaaacaa aaacaactct tgtagtaatc gaacaatctt
    44821 tagatagacg aaaaagcaag atggccaatg caagaataga gctggaagaa ataagacaac
    44881 aagcagaaaa gctcgaacaa caattagctg tctacaaaca gcagcagaca gaaaaaaata
    44941 atcagctcca gcaactgcaa ggagaaatca aaagtttaga acaacaattg caagatacgg
    45001 atagtaactg tttagaagag gagatacgac gacttgaaaa acagttagaa gaagactcga
    45061 atcaactagc tgttgagcag aaagcaggag tgcaattgtt gcaagatctt catacgcttc
    45121 ggacacagct tgatcttcaa catgaacaaa tagaaaaaat agatgaaaaa atagaaaagg
    45181 aacaagaaag aatagctaaa aagttagaaa gccagtcgta tttttcaaca attgaagaaa
    45241 tctgtgcatt tgcacaacag caagcacaat ataaagaaaa ccaacgtaca gttacttctt
    45301 atcatgaaga gcaattagtg acagctgacc ggctgcgtca attagaaaat atccagctaa
    45361 ctgagacatt cccagataca gaagagatca gccaacagtt agaagaaata agacgtatga
    45421 tcgaaataaa gcaagaaaag atatacggtt tacaagaaca aaaaattagt aatcaaaaaa
    45481 tctacgatga atgtctttcg atttatcaca caagccagac gaaattagat gagcttacgc
    45541 aattacagca gctttcgcaa acattcaacg gagaaaatcc taagaagact agtttggaac
    45601 ggtatgtgct tcaggtttat cttcaagaag tgttacaagt agcaaatgat catctccaac
    45661 gtttaacgaa aaatcgttat caatttgagc tagcggatac cattggcagt tatcgaggga
    45721 aaaccggatt agaaatcaat atctatgatg atcaggcagg tatgtctcga agtgcgcata
    45781 ccctgtctgg cggtgagagt tttattgcag cactttcttt agcattagct cttgccgatg
    45841 tgatccagac acaagctggt ggtgtagcaa tcgatgcatt gtttattgat gaaggatttg
    45901 ggtcattaga tgaagaagct ttggaaatgg caatggaagc tttagaaacg atcgaaagtg
    45961 aaggaagaat gatcggcatc attagtcatg taagagaatt gaaagaacga gtattacaac
    46021 aaataagaat tgatacagaa ggtagcggac aaagcaaaat acgctatcaa cttggataaa
    46081 aagggagatt ggtaactatg aaatggagta ttccagaaaa agtcattgca cgtggacgtc
    46141 aatacttgca agaaggccga gtgttatcgg ttgtccctga ccaagaagaa aacgtgtggc
    46201 atgcagaagt cttgggcagt gaattgtata tggttgattt ggatggtaca gcaaaagaga
    46261 tagattactg ccaatgtccc tattgggagg aacataaata ttgtaagcat acagttgctg
    46321 tggaacttta tttacggtcg aaaaatctat ctcgtatcat gaaaaaagac cagccaatgc
    46381 tagaaccggt aaaagcaaca agtgagggag aaatcattgc aaaaggtttt tctcgtctaa
    46441 aaacacaaaa caatcctcaa gcggtgcaac ctttgattat tgaataccaa gtagaaacga
    46501 ttgagactaa tcagtatcac ccagagcttt ctattttggc actttacttg cgtatcggtt
    46561 cattggcatc accgaaaaaa acatatatcg taaaaaacat ctatgatttt ttacaagctt
    46621 acacagaaaa agaaacttac actgttaata aacagtacca gtttcgtcta gataaacggg
    46681 cgtttcaaaa agaagatcaa aaaatactca cgcatcttgc aggaagtgct cagacgcatc
    46741 aattattagg tacgaacggc ttacaagtca aaggaaaact agataaaaga tatctgcttc
    46801 tcccagtaga acaagcccgc ttcctgttag agcagatgaa tcagactaca cgtttgcatc
    46861 tgcagataaa tgaacaaaac gatcaaggaa tacaattttc cgatcaaaac aaacctttat
    46921 cattcaaagt ggaaaaattt ggagaagatt atttgctgca agcattgaat gattttgatt
    46981 ttttctttat gcattatcag tggggagtta tccaagggac attttattct ctgacgaaat
    47041 atcaacaaac ggtttatcaa acttggaaac aattgattcg ccgattggag aaaccagaag
    47101 tagtgttcaa gaaaaaagaa ttaccgtctt tgtttaaaga gattatccct ctgctgtcag
    47161 agatcggcga cgtagctatt gcccctgcag tagaagagga agtagcagat atccccgtcg
    47221 aatttatttt ctttttccga aaagcaaaag gaaaaatcca agctcgagtc gatttcgtct
    47281 atggtgaagt catctattcg actgacccga agcatgaggt ctcgacagaa caggcttttc
    47341 aggtgttacg tgacacagta gaagagaaac gagtacttga tctattcaga atgtatcgct
    47401 accagaaaaa tgaaactggt tatgaacgtc cattgccaag cggggaagaa ctatatgcct
    47461 ttttccgcac agaactatct gtcttccgtc agtttggtga agtcagactt ggaagaaaat
    47521 tgcgagagtt gtatttagat gcgcataaac atcaaccgaa aatcgaagta accgagaccg
    47581 gttcatggct cgatattcga ttcgatgtga cagggataga ggagcaagaa atcgatcatg
    47641 tcctgcaaag cttgctgaga aatgatgctt tttatacatt agaaaatggt caggttcttt
    47701 cttttgattc agaagaattt caacaaacaa gccaagtgtt gcaacaatta cgggaatcga
    47761 tacgtacaga agaaggaacg atccatgtac ctaaaaatca aggattgatc attcaaaatc
    47821 aattggaaaa aagcaatgct acttttagtg aatcgttcca aacgatggta caagatctca
    47881 ttcatccaga aagataccag gcacagcttc ccaaaggatt gaatgcaacg atgagagatt
    47941 atcaaaagca aggttttcga tggttgaaga tgttaggtca ttatcagttt ggcggtattt
    48001 tagccgatga aatgggactt gggaaaacac tacagacaat tgccttttta ctttcggaaa
    48061 aagaagaacg gaaaagcttc tctgctttga tcgtagcgcc tgcgagctta atttacaact
    48121 ggcaggcaga ggtgagaaaa ttcgcccctt ctttaagtat ccaagtaatc aatgggaata
    48181 aaaaagaacg tgaagaatta ctggctaaag acacagatat ccgagtgact tcttatgcca
    48241 gcttaagaca ggatctagct aattatcagt cgcaaaaaat tgattatttg attcttgatg
    48301 aggcacaaat ggtcaaaaat agcagtacga aaactgcaca agcattgaga gaattagctg
    48361 tacctcagcg gtttgcactg agtgggacac caatcgagaa taatttagaa gaattgtggt
    48421 cattgtttgc aacgattatg ccaggatttt tcccgactaa aacaaaattc agggaacttg
    48481 ccgcagaaga aattgcgcaa atgattcgac cgtttatttt acggcgagat aaacaaactg
    48541 tgttgaaaga cttgccggaa aaaacagaga tgaatctata ttctgcactt acagaagaac
    48601 aaaaaactgt ttatttagct tacttgcgtc aaatgcgtga agaaatcaca tcaatggatt
    48661 ctgaagcatt caagaaaaac cggatcggga tacttgctgg tctgacaaga ctgcgacaaa
    48721 tttgctgcga tcctcgtctg tttattgaag attaccaagg cggttccggc aagctcgaac
    48781 aagtaaaaga tctactggta gctgccaaag agaacaaacg aagagttttg ctgttttctc
    48841 aatttacagg gatgctgact atcttgcaag aagagttagc cgagctaggg atctcgacat
    48901 tttatttaag aggcagtacg aaaccacaag atcggttaag tatggtagac gcatttaatg
    48961 ctggcgaaaa agatgttttt ctgatttcct taaaggctgg gggaactgga ttgaatctca
    49021 cgggagcaga tacggtcatc ttgtatgacc tttggtggaa tcctgccatt gaagaacaag
    49081 ctgctggacg tgcacatcgg atcggacaaa aaaatgtggt agaagtgtgg cgtatgatct
    49141 ctgaaggaac gattgaggaa cgcatggatt cactccagca agaaaaacgc gaactgttcc
    49201 aaaaagtgat ccaaggtaat gaagaacagt tgacgaaact gactgaagaa gacatccgca
    49261 tgattttaag tgtaggagaa atcgaggagt aagagactcg aaaaagcgtt cagtaatggg
    49321 taagaaagcc tttctaaaga agcgattgtt tatttgattt aaaaaatagc aaggaggctg
    49381 ggattccatt ttatcccagc ttccttgcta tcattttttt atttctattt ttaaaggtta
    49441 tacaaagatt gtcttcattg tctctaacag ctgatcaaaa gaagaaatct cgatatcgca
    49501 aattggagtc tgttcaattt tgcgttctcg atggttgaac caaagcgctt gccagttagc
    49561 actttttgca cctagaacat cattgtcgta attatcgcca acgtataagg tctcttctgg
    49621 aagcatatga aattctttct cagccagctg aaaaatctct ttttctggtt tttggaaacc
    49681 tgttgcttga gaaatgagca tattgcggga aggaatccaa ttgttcaatt gaagttgctt
    49741 cagttttttt gtctgatggt cagtcggccc atttgtgata attcctaatg ggacattctt
    49801 ttccttcaag aaatcgaggg tttttttgac ctcctcatgc aagcagatat tatcaagttc
    49861 ttcctcgtaa attttttgaa ataagaggcc atcttcttcc gtaatatgag ggtagtctaa
    49921 atcttttagt gattggctga tccgatgtgc tcgcatatat tctagtgtcc aatctcctgc
    49981 cattactttt gggaagtttt catcactatg atggcggaac cggatatata gtggatgcat
    50041 atctgcatct gaaacaagcg gaacgactct tttgaccgcg tttcgaaatg gctgctgctg
    50101 atcatacatc gtatcatcaa catcaaacac aactgattta atcaaaattt tacacgcctt
    50161 tcggttttca ttgatttttt cacaaatcct tgactattgt aacagaaaaa attgtctaaa
    50221 aaagctgata aataaaggtt tttcttacta tatttctatt tccttgacag aataaggttt
    50281 ttatatttca gtttttttca ctaaaaatcg tgaaacacgt caataagttt gatgaacaaa
    50341 ttaatcatta tggtagaagt cgattgattt attttaatgc aatctctgtg gaattttcga
    50401 aagatttatt tttttaatac agtctcaata taactattca aacataaaat aacgttattt
    50461 gatttatttg tttatagtaa attttattta gtaatcattg ctttaaaata ctacgcttat
    50521 ataacagaga aaataagcgt tataataaag aacagtatta tttaaaatga cagaaaacat
    50581 tactattaaa gaaataaaaa acataaaaaa tcttaagaaa tcggtttcat ttagcttttg
    50641 agataaaata atagtacagt tttatacgct gtttcatatg tttagaaaat ttgaattaga
    50701 tttgaaaact ctaattttta aatgataaaa aaagcgagaa tgctggtttt actgtatatt
    50761 aaccttagat aactttcatt tttttaaaaa aattatgaaa aaaaggtagc tatttaaaag
    50821 ttgattatgt ataatagaaa cagatgggag attattatga tcttctgtta caaaaaagtg
    50881 attcttatca cgaaaaggag tggtgcacgt tgttaacatt atatacttcc ccaagttgta
    50941 cttcctgcag aaaggctcgt gcgtggttgc aagaacatca gattccattt gttgaacgta
    51001 atattttttc agaaccattg aacagttctg aattaaaagc gattttacaa atgacagaag
    51061 acggaacaga agaaatcatc tcgacacgtt ccaaagtatt ccaaaaactg aacatggatt
    51121 tagatgatct gccgttacaa gaattattgg aacttgtgca aaacaatcct ggattgcttc
    51181 gccgtccaat catgattgat gataaacgtc tgcaagttgg ttttaatgaa gatgaaattc
    51241 gtcggttcct accgcgcgat gtacgacaat tagaattacg tcaagcacaa ttgatggctg
    51301 gattatagaa ggataatacg aaacttggaa aagactggct ctttgtcaaa tgatgttggt
    51361 aaagagaaat cttagaataa tttggacaga acagaaagaa gaaaacctct ttctgttctg
    51421 tttatttggt taaaaatgtt ttgc
//
LOCUS       DACDUU010000010        51303 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.010, whole genome shotgun sequence.
ACCESSION   DACDUU010000010 DACDUU010000000
VERSION     DACDUU010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.010"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..446
                     /locus_tag="IWL30_000621"
     CDS             <1..446
                     /locus_tag="IWL30_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705411.1"
                     /translation="IKSLVRIIHQLIKENHILLFCPSITMMHRLKATIQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            443..1162
                     /locus_tag="IWL30_000622"
     CDS             443..1162
                     /locus_tag="IWL30_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAP9705412.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            1290..1841
                     /gene="raiA"
                     /locus_tag="IWL30_000623"
     CDS             1290..1841
                     /gene="raiA"
                     /locus_tag="IWL30_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAP9705413.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            2063..4597
                     /gene="secA"
                     /locus_tag="IWL30_000624"
     CDS             2063..4597
                     /gene="secA"
                     /locus_tag="IWL30_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAP9705414.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDLGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            4723..5824
                     /gene="prfB"
                     /locus_tag="IWL30_000625"
     CDS             join(4723..4785,4787..5824)
                     /gene="prfB"
                     /locus_tag="IWL30_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAP9705415.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            5929..6615
                     /gene="ftsE"
                     /locus_tag="IWL30_000626"
     CDS             5929..6615
                     /gene="ftsE"
                     /locus_tag="IWL30_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAP9705416.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            6599..7492
                     /locus_tag="IWL30_000627"
     CDS             6599..7492
                     /locus_tag="IWL30_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9705417.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            7644..9092
                     /gene="cls"
                     /locus_tag="IWL30_000628"
     CDS             7644..9092
                     /gene="cls"
                     /locus_tag="IWL30_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP9705418.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEIAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYILQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLIYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            9429..10286
                     /locus_tag="IWL30_000629"
     CDS             9429..10286
                     /locus_tag="IWL30_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAP9705419.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            10401..11321
                     /gene="pstC"
                     /locus_tag="IWL30_000630"
     CDS             10401..11321
                     /gene="pstC"
                     /locus_tag="IWL30_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAP9705420.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            11321..12205
                     /gene="pstA"
                     /locus_tag="IWL30_000631"
     CDS             11321..12205
                     /gene="pstA"
                     /locus_tag="IWL30_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAP9705421.1"
                     /translation="MNAKRSDKIATGVLYVVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            12220..13026
                     /gene="pstB"
                     /locus_tag="IWL30_000632"
     CDS             12220..13026
                     /gene="pstB"
                     /locus_tag="IWL30_000632"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP9705422.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            13042..13800
                     /locus_tag="IWL30_000633"
     CDS             13042..13800
                     /locus_tag="IWL30_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP9705423.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            13813..14490
                     /gene="phoU"
                     /locus_tag="IWL30_000634"
     CDS             13813..14490
                     /gene="phoU"
                     /locus_tag="IWL30_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAP9705424.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            14727..15758
                     /locus_tag="IWL30_000635"
     CDS             14727..15758
                     /locus_tag="IWL30_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP9705425.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTNQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKHGLFALGSLIINIVLLLLLLLIFTKIEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNLWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            15755..16522
                     /locus_tag="IWL30_000636"
     CDS             15755..16522
                     /locus_tag="IWL30_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP9705426.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTTIELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            16622..18190
                     /gene="liaX"
                     /locus_tag="IWL30_000637"
     CDS             16622..18190
                     /gene="liaX"
                     /locus_tag="IWL30_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAP9705427.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            18490..18807
                     /locus_tag="IWL30_000638"
     CDS             18490..18807
                     /locus_tag="IWL30_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAP9705428.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            18807..19157
                     /locus_tag="IWL30_000639"
     CDS             18807..19157
                     /locus_tag="IWL30_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP9705429.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            19324..20262
                     /locus_tag="IWL30_000640"
     CDS             19324..20262
                     /locus_tag="IWL30_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAP9705430.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            20277..21107
                     /locus_tag="IWL30_000641"
     CDS             20277..21107
                     /locus_tag="IWL30_000641"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP9705431.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            21151..22176
                     /locus_tag="IWL30_000642"
     CDS             21151..22176
                     /locus_tag="IWL30_000642"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP9705432.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            22191..23129
                     /gene="galU"
                     /locus_tag="IWL30_000643"
     CDS             22191..23129
                     /gene="galU"
                     /locus_tag="IWL30_000643"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP9705433.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            23254..23694
                     /locus_tag="IWL30_000644"
     CDS             23254..23694
                     /locus_tag="IWL30_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAP9705434.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            23696..24298
                     /locus_tag="IWL30_000645"
     CDS             23696..24298
                     /locus_tag="IWL30_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP9705435.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDTKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(24357..25463)
                     /locus_tag="IWL30_000646"
     CDS             complement(24357..25463)
                     /locus_tag="IWL30_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP9705436.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKILP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            25673..26674
                     /gene="ccpA"
                     /locus_tag="IWL30_000647"
     CDS             25673..26674
                     /gene="ccpA"
                     /locus_tag="IWL30_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAP9705437.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(27011..29356)
                     /locus_tag="IWL30_000648"
     CDS             complement(27011..29356)
                     /locus_tag="IWL30_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP9705438.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSISDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLSKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            29635..30537
                     /locus_tag="IWL30_000649"
     CDS             29635..30537
                     /locus_tag="IWL30_000649"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAP9705439.1"
                     /translation="MLLGSHVSMSGKKMLLGAAAEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            30750..32525
                     /locus_tag="IWL30_000650"
     CDS             30750..32525
                     /locus_tag="IWL30_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAP9705440.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(32591..34177)
                     /locus_tag="IWL30_000651"
     CDS             complement(32591..34177)
                     /locus_tag="IWL30_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAP9705441.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETVEEIE"
     gene            34428..34652
                     /locus_tag="IWL30_000652"
     CDS             34428..34652
                     /locus_tag="IWL30_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAP9705442.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            34794..36545
                     /locus_tag="IWL30_000653"
     CDS             34794..36545
                     /locus_tag="IWL30_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9705443.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSVKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIISAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            36545..38329
                     /locus_tag="IWL30_000654"
     CDS             36545..38329
                     /locus_tag="IWL30_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9705444.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLTKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVECGNHETLLAQNGLYADMYKLQSSGV"
     gene            38787..39638
                     /locus_tag="IWL30_000655"
     CDS             38787..39638
                     /locus_tag="IWL30_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9705445.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELYQVIQKKYKDTFGCVEKIADFLAEIFNYQISTDEKMYLTIHTARL
                     VHENRCK"
     gene            39735..41582
                     /locus_tag="IWL30_000656"
                     /pseudo
     CDS             39735..41582
                     /locus_tag="IWL30_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
     gene            complement(41623..42525)
                     /locus_tag="IWL30_000657"
     CDS             complement(41623..42525)
                     /locus_tag="IWL30_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9705446.1"
                     /translation="MLLREKIKQISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLDETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            42755..44158
                     /locus_tag="IWL30_000658"
     CDS             42755..44158
                     /locus_tag="IWL30_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP9705447.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            44158..45591
                     /gene="ascB"
                     /locus_tag="IWL30_000659"
     CDS             44158..45591
                     /gene="ascB"
                     /locus_tag="IWL30_000659"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP9705448.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            45736..46662
                     /gene="trxB"
                     /locus_tag="IWL30_000660"
     CDS             45736..46662
                     /gene="trxB"
                     /locus_tag="IWL30_000660"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAP9705449.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            46876..48603
                     /locus_tag="IWL30_000661"
     CDS             46876..48603
                     /locus_tag="IWL30_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP9705450.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EENHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            48675..48968
                     /locus_tag="IWL30_000662"
     CDS             48675..48968
                     /locus_tag="IWL30_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP9705451.1"
                     /translation="MPDTQKSKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(49060..49920)
                     /locus_tag="IWL30_000663"
     CDS             complement(49060..49920)
                     /locus_tag="IWL30_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAP9705452.1"
                     /translation="MTSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            50012..50440
                     /locus_tag="IWL30_000664"
     CDS             50012..50440
                     /locus_tag="IWL30_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP9705453.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGKIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            50602..50778
                     /locus_tag="IWL30_000665"
     CDS             50602..50778
                     /locus_tag="IWL30_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAP9705454.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            50805..51251
                     /locus_tag="IWL30_000666"
     CDS             50805..51251
                     /locus_tag="IWL30_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAP9705455.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT        16656 a         8974 c        11015 g        14658 t
ORIGIN      
        1 agataaaatc tttggtgcgg atcattcatc aattaattaa agaaaaccat atccttttat
       61 tttgtccaag tatcacgatg atgcatcgtt tgaaagccac aattcaagag gctcttccga
      121 gatatgagat cacagatgtt tcttcaagag acaatcagcg ggcggaaaaa gtccagaaaa
      181 tgcgagaagc aaaatatgac atactgttaa ccacgatgat ccttgaacgt ggagtcacat
      241 ttgaaagaat atccgttatc gttttaggag cggatcatcc ggtttttact aaatcttctt
      301 tggtacaaat cgcaggaaga gccgatcgta aagggcctta tacgaacagt caagtatact
      361 ttttctatga agaaaaaact gcagctattc gaaaagcttg tcaggaaatc aaagaaatga
      421 atgaagaagg gaagaagatg ctgtgaggtg cagttgctgc agtaaagata tcgtccaaaa
      481 tttaacattc agcgagatta ttggtgtttc gaaatacaga ttacctcaga cttgttcagt
      541 ttgtcgcgca caatttcatt tattggaaac aagaggctgt tctggctgtc aaaaaccaat
      601 ccatttcaat ctagagagta cgaaaaagaa agtaaagtct attcccgttt actgcagtga
      661 ctgtcaagaa tggaagaaga aatatccgac ctatgatttt cagcaccgtg ccttttttgt
      721 gtacgatgaa aatatgaagg cctggctcaa tcaatacaag tttatgggag atatccgact
      781 agcaggcacc tttgcatata aatggacgca gataaaaaaa gaatacccga catacatcta
      841 ttgtccaata cctttatctg aaaaaaggcg aacagaaaga ggattcaatc aagtaagcga
      901 gatgttgaaa gcagcaagaa ttccttttca gatgctactc aaaagagaga agcatctgct
      961 ggctcaagca aaaaacacgc gaaaggaaag attggagatg cctcaacctt ttgaacttgc
     1021 agtcaccttt tctaagatac aaaataaaaa aatattgatt attgatgatg tctatacaac
     1081 aggacagact cttttccacg cagcagattg tttgcaggtc gcacagccga aaatcatccg
     1141 aaccatttct ttagcaagat gaattagatg tggaaatttg atagcgattt aatttgcaaa
     1201 taacctagag ttcgatataa tagaattaag aagagggaaa gagtggtcct tctccttctt
     1261 gattgtggta gacgaaaggg gtaattttta tgtttagata taatgtacgc ggcgaaaata
     1321 tcgaagtaac tgaagcgatt cgcgactacg ttgagaaaaa agtaggcaaa ctagaacgct
     1381 atttcagtga cgcacccgat gcaacggcgc atgtgaactt aaaagtttat acagaaaaaa
     1441 cagcaaaagt ggaagtaacg atcccattac cttatcttgt attacgtgcc gaagaaactt
     1501 ctccagattt atacgcaagt atcgatttgg ttgttgacaa attggaacgc caaatccgca
     1561 aattcaaaac aaaaattaat cgtaaatccc gtgaaacggg catgaatact gcagatgcta
     1621 ctgctttatt caatgaagag gacgaaaacg aaaatgattc tgaattagat atcgtacgta
     1681 cgaaacgcct ttcattgaaa ccaatggata gtgaagaagc agtcttacaa atgaacatgc
     1741 tgggacataa cttctttatt tttgaagatg cagaaacaaa tggtacaagt atcgtttacc
     1801 gccgtaaaga tggaaaatac ggactgattg aaacagactg atcagcctta cttaaataac
     1861 aacgaaaact tgcagcagct actgacagct gccgcaagtt tttttgagtt tcctcctctt
     1921 ttagatgaaa tatgaataaa ttatgaagtt cttactttaa tacgcttaag atatttcaat
     1981 tatctataac taatgataaa atgaaaagta aagtctacat agtctggata atacacagaa
     2041 ggaaaaggag attacacaac gcatggccaa tttgattcgt tcacttatcg aaaacgataa
     2101 gaaagaacta aaacgtttag atagcattgc aaaaaaagtt gaagcatacg ctgaccaaat
     2161 ggcagcatta actgatgaac aattacaggc aaaaactgct gaattcaagg gcagatacca
     2221 aaaaggagaa acattagatc aattacttcc ggaagctttt gcagtcgtcc gagaagcagc
     2281 aaaacgtgta ttggggttat atccacacca cgtacaattg atgggtggaa tcgtgcttca
     2341 cgatggaaat atccctgaaa tgcgtactgg tgaaggtaaa accttgaccg caacgatgcc
     2401 agtatatcta aatgctctga ctggcgaagg ggtacacgta gttaccgtca atgaatattt
     2461 agcaacccgt gactcgacgg aaatgggcga actttataat ttcttaggat taacagtggg
     2521 gttgaatatc aactcaaaat cttcggatga aaaacgcgag gcttataatt gtgatatcac
     2581 ttatagtacg aataatgaat taggatttga ctacttgaga gacaatatgg tcgtttaccg
     2641 ccatcaaatg gtacaacgtc cgctgaatta tgccattgtc gatgaagttg actcaatctt
     2701 gattgatgaa gcgagaacgc cgctgatcat ttcagggcaa gcagaaaaat ccacagcttt
     2761 atatacaaga gcagacaact tcgtgaaacg tttgaaagaa gaagaagatt ataaaatcga
     2821 tgtacaatca aaaacaatca gtttaaccga agcagggatc gaaaaagcag aagaaacatt
     2881 cggtttggat aacttatacg acattgaaaa tactgcattg acccatcact tggatcaagc
     2941 attgagagcc aactatatca tgcttttaga tatcgattat gttgtccaag acggaaaagt
     3001 actgattgtt gaccagttca ctggtcggat catggatgga cgccgttatt cagatggact
     3061 tcaccaagca atcgaagcta aagaaggcgt agagatcgaa gacgaaacga aaaccatggc
     3121 gacgatcact ttccagaact atttccgtat gtacaagaaa cttgcgggaa tgactggtac
     3181 agctaaaaca gaagaagaag aattcagaga aatttataac atccaagtat accagattcc
     3241 gacaaatcgt ccggttatcc gtgatgatcg tccagatttg ctttacccaa cattacaaag
     3301 caagttccac gctgtcgtgc aagatatcaa agatcgctac cgcaaaggtc aacctgtgtt
     3361 agtcggaact gttgcggttg aaacatctga gttattatca gatatgctga atcaagaacg
     3421 tattcctcat gaagtgctga atgcgaaaaa tcactttaaa gaagcagaga tcatcatgaa
     3481 tgctggtcaa aaaggtgctg tgacgattgc taccaacatg gctggtcggg gaacagatat
     3541 caagttagga cttggggtaa gagaacttgg tggtctagca gttatcggga cagaacgaca
     3601 tgaatctcgt cgtatcgata accagcttcg aggacgtgca ggacgtcaag gagaccttgg
     3661 tatgtcacag ttctacttgt cattggaaga tgatttgatg aaacgattcg gttcagaacg
     3721 aatcaaagct ttcttagatc ggatgaagat cggtgaggaa gatgctgtta tccaaagcaa
     3781 gatgctgacg aaacaagtag aatctgcaca aaaacgtgta gaaggaaata actatgacac
     3841 acgtaaaaac gtcttacaat acgatgatgt gatgcgtgaa caacgtgaag taatctataa
     3901 acagcgacaa gaagtaatca tggaagaaaa aagcttatcc aagccactaa tgaacatggt
     3961 caaacggaca atcagtcgtg tagtagatgc gcatacacaa ttagagaaag aaaattggaa
     4021 tttggaaagt atcatcgatt ttgccggcag tactcttgtc catgaagata gtattactct
     4081 tgcagatatc gaaggaaaaa cagctcagga gatcaaagag tatctaaacg aacgagcaca
     4141 agaagtattt aataccaaag cggctcaact aaatggtcca gagcaactgt tggaatttga
     4201 aaaagtcgtg atcttgcgtg ttgtggactc taaatggaca gaccatattg acgcaatgga
     4261 tcaattacgt cagtcaatcg gcttacgtgc ttatggacag aacaatccat tagtcgaata
     4321 tcaaacagaa ggctataaga tgtttgaaga tatggtaggc gccatcgagt atgaagtcac
     4381 tcgaatcttt atgaaagctg agatccgtca aaacgtacag cgtgaacagg tttctcaagg
     4441 aacagcttct cactcagaag acggagatgc caaagaaaat agtaatacta gcgcgaaaaa
     4501 acagccaatc catgtgaaca aggtcggaag aaatgatcct tgtccttgtg gaagcgggaa
     4561 aaaatataag aactgtcatg gaaaaaatgc aaactaattt ctgacagcag gattggtgag
     4621 acaaattctt tttttaagaa ttcgtctcac cttctttctg ttatttgctg attctgttag
     4681 actagatata atgaaaaaag tatagtgaaa gaaggaaaaa acatggaaat atccgaaatt
     4741 cgaaatctcc tgacagaaat gcaggaaaag gtcacaagtt tcaggaggtc tctttgactt
     4801 agatcagctg gaagaagaca tcgcggaagc agaagcaaaa atggctgaac ctggtttttg
     4861 ggatgacagc gaagccgcac aaaaagtgat cagtgaaaat aacgctaata aagaaaccta
     4921 tgaccaattc cattctcttg ccgaagaact agaagaaatc gaattgatgg tggagatgct
     4981 gcaagaagaa cctgatgcag caatgcaaga agaagcagaa acacgtatca atgcattaga
     5041 tgaaaaaatg aagacgtatg aattatctat gctgttagac ggaccttatg atcgaaacaa
     5101 tgcgatcgtc gaattgcatc caggggctgg cggaacggag tcccaagact ggggcagcat
     5161 gcttttacgt atgtatacac gttgggcaga gcagcatggc tatcaagtag aaacactaga
     5221 ttatcaagca ggtgatgaag cagggatcaa gagtgtgacc ctgctgatta aaggacataa
     5281 cgcttttggt tatctaaaat cagaaaaagg tgttcatcgc cttgtacgga tttcgccttt
     5341 tgactcagcg aaacgacgtc atacttcttt ttgttcggta gatgtgatgc cagagcttga
     5401 tgaaacgatc gatatagaaa tcaatccaga tgatttgaaa atcgatacgt accgagcaag
     5461 tggtgcaggt ggtcagcaca tcaataaaac agaatctgct gtacggatca ctcacttgcc
     5521 tacaggaaca gtagtagcta gtcaggcaca acgctcacaa ttgaaaaacc gtgagcaagc
     5581 gatggggatg ttaaaagcta agctttatca attagaagtg gaaaagaaag aacaagaagc
     5641 agctgcactt cgaggagaac aaaaagaaat cggctggggt tcacagatcc gttcttatgt
     5701 tttccatccc tattctatgg taaaagacca tcggacaaac gtagagacag gaaatgtcca
     5761 agcagttatg gacggtgacc ttgatttatt tattgacggc tatttaaaaa tgcatctaca
     5821 ataagaaccc attgttaaat tgaaataaaa ttgtaagatt ttgcaagcat gttgaaacat
     5881 tgccatgcta taatagctaa agactgaaaa agatatggag agaacgccat gattgaaatg
     5941 aaggatgtaa tgaagaagta ctcaaatggt acgaccgcta tccgcaatct ctcgattgtc
     6001 atcgaacaag gagagtttgt ctatgtggtt ggtccgtctg gagccggtaa atctacattc
     6061 atcaaattga tgtatcgaga agaaaaagct tcaaaaggaa ctttgaacgt cgcaggtcat
     6121 gatctaatag caatcaaaaa ccgcgaagtt ccttatctac gcagagaaat cggcgtggtg
     6181 ttccaagact ataaattatt accaagaaaa acagtatatg aaaatgttgc gtacgctatg
     6241 caagttatcg gcagaaaacc tcgagatatc aaaaaacgag taatggaagt gctggacctt
     6301 gttggtttga aacacaaagt tcgagtcttt cctagcgaat tatccggtgg ggaacagcaa
     6361 cgtgtctcta ttgctcgggc aatcgtgaat acgcctaaag tacttatcgc ggatgaaccg
     6421 acaggaaatc tggatccaga aaactcatgg gaaatcatga aattattaga tcggatcaat
     6481 gcccaaggaa ctactgtcgt gatggctaca cataacagta cgatcgtaaa tacgattcgg
     6541 caccgagtaa ttgctatcga aaacggccgc attattcgag atcaagcgga aggggaatat
     6601 ggatacgatg attagaacct ttttccgcca tttattagaa agtatcaaga gtttgaaacg
     6661 taacggctgg atgacagttg cttcagtcag tgcggtaacg atcacgttga ctttagtcgg
     6721 tgtattcatg gcagttatca tgaatgcaac taaattagct caggatatag aaggaaacgt
     6781 tgatgtatct gtattcgtag atatcggtac aaaagacgaa gagatgaaaa agctagaaaa
     6841 agaattgaat gacttagatc atgttaaaaa agttgaattt tcaagtaaag aacaggaatt
     6901 gaaaaaaatc caaaacgaaa tgggtgattc ctggaaattg tttgaaggtg acaataaccc
     6961 gttatatgat gtgtatgtag taagtgctac tgatccttct tatacaaaac aagtagcaga
     7021 agaagcagca gatttgaaga atgtttctcg ttctgattac ggtggagctt cctcagatcg
     7081 gattttccaa atcgctggtg cagttcggac atggggatta gttgcagcgg cattgctgct
     7141 gttcgtagcg atgtttctca tttcgaacac gattcggatc acgattttgt ctagacagcg
     7201 tgaaattcaa atcatgcgtt tagtcggtgc aaaaaatgga tatattcgct ggccgttttt
     7261 ccttgaaggt ggctggatcg gtctgatcgg tgcgattctt ccgattttga tcatcacatt
     7321 tggttatgca tggtttttcc aattgatcaa tccatcatta ttacgttccc attattcatt
     7381 gattcatcca caaaatatcg tttggaaaat caatctttta atggtcggca ttggtgtgat
     7441 cattggttca cttggctcga tcatttctat gagacgtttc ttgaaattct aataccacaa
     7501 tataaataaa atgagaaagt tggacagttt cgttccagct ttcttttttt attggctagg
     7561 agaatagaag gaagcggctt ttttcccttt actttaaaaa acaacttggg tattcttaag
     7621 ttggtagagg aagaggtgaa gccgtgaaaa ttatcgttga caattttttt acgatccttt
     7681 tgattatgaa tattttgctt tcctttatca tcgtttttcg agaaagaaag gaaactgctc
     7741 aaacatgggc atggctgctc gttttgatgt tcattccagt tgtaggattt attttatata
     7801 tttttttagg aagagggatt tccaaagaga aaatctttga tttgaagatc caagggaaaa
     7861 tcggtaagaa cctggaaatt gaagaagaca aacaggcatt gatgcgaggg ctttatcctc
     7921 atccgcctac tggacaagta gatgtgaaac aattgatcta tatgctcacg gtatttgaaa
     7981 gtacgctcta tacgactaaa aatgaaatca ctttatttac agatggtcgt gaaaaattcg
     8041 atgcgctgat tgaagatatc aagcaagcag aagaccatat ccacttccag tattatattt
     8101 atcgcagtga tgcattaggt gaagaagttc gagatgcatt gacagaggcg gcaagacgag
     8161 gggtaaaagt cagagtcctt cttgatgcat ggggttccac acaagtttct tccagctttt
     8221 ttcagaattt gaaaaaagca ggcggagaaa tcgcattttt ctttcctcta tttgttccgt
     8281 atattaatcc tcggatcaat tatcgcaatc atcgtaaaat cgtagttatt gatgggaaaa
     8341 tcggatatac aggtgggttc aatgttggaa atgaatatct aggattggta aagaaatttg
     8401 gttattggcg agacaatcat ttgcgtattt acggagaagc ggtgtatatc ttgcaaaacc
     8461 gttttttgat ggactggaat tctcagcata caaaagaagt aggctattct cccaaatttt
     8521 ttccgtccat tcactctact ggtgatatag ctgttcaaat cgtgacaagc ggaccagata
     8581 ccgaacatga acaaatcaaa atgacttatt tgaaaatgat ttcgttagcc aaacgggaga
     8641 tattgatcca aacgccttat tacataccgg atggatcgat tcatgaagca ctgaagttgg
     8701 ctcttctttc aggcgtaaaa gttcatatcc aaatacctaa caaaccagat catcttctcg
     8761 tttactgggc aacgtattct ttcgctgcag agctgatcga gtatggagca aggatcgaaa
     8821 catatgagaa tggatttatt catgcaaaga cgatgattat cgatggtggc attgtttcag
     8881 ttggctcagc aaatatcgat gttcgttcat ttcgcttgga ctttgaggtc aatacactga
     8941 tttatgatga acgaatggct tcacgtgtaa gaaaagcatt ttttgaagac tcgaaaattt
     9001 ctacgcatct cacaaaagaa atgtatgaaa accgaggaat aatcatcaag atgaaagaag
     9061 gattagcaag attgatttcg ccgctccttt aaaacacaaa aaggacatga gaaccatcaa
     9121 cagatggaga tctcgtgtcc ttttctaacg gggccatttc tgagagtacg tcagaaatgg
     9181 agaaagcgtc tcgcatgagc gaaattaact ttggggtatc tcttgttttt tggggaaagc
     9241 aagaaataag ctaatatatg ctattaaaag agacacattt caaagccagt atagcataga
     9301 aacttttaat gagatagttt tttttacaat tatgaggatt tattttgtta tttaatgatt
     9361 ttgattcgtt ttcatcaagt aatgaaaatg ataaaataaa catactttag gattaaagga
     9421 gatttctaat gaaaaaactt aaattattct taccaataat aggattagct attcttttat
     9481 caggctgttc aaagtggatt gatcgaggag aatccattac ggctgttggc tcttctgctt
     9541 tacagccttt agtagaaaca gttgctgaaa cttaccagac agaaaatcct ggaaaattca
     9601 tcaatgtaca agggggcgga agcggaactg gtttgtctca agtacagtca ggagcagtcg
     9661 acatcggaaa ctctgatttg tttgcggaag aaaaatcggg gatcgatgcc tcagcattag
     9721 tcgatcatcg agtagcagtt gttgggatca ctcctattgt gaacaaggat attggcgtaa
     9781 aagacatcag tatggaaaac ctgaaaaaac tatttacagg cgagatcaaa aactggagtg
     9841 aagtcggtgg aaaagatcag ccagtagtta tattgaaccg tgcttcagga agcggaaccc
     9901 gtagcacgtt tgaaaaatgg gtattggacg gagaaacagc tgttcaggca caagaacaag
     9961 attcaagcgg aatggtgcgc cagatcgttg ccgacacacc cggtgcaatc agctatgttg
    10021 ctttttctta tgtttccgat gatgtagata cgttgaatgt taacggtgta gaaccgacag
    10081 atgaaaatgt gacaacgaac aaatggacga tttgggcata tgagcatatg tacacaagag
    10141 atcatccaaa aaaactgaca aaagaatttt tagactatat cttatctgat gaagtacaag
    10201 ataacatcgt tggagaacta ggttatatcc ctgtttctca tatgaaagtc gaacgtgatt
    10261 ggcaaggaaa tatcgttaaa gagtaatctt tacacaaaat ttacaaactt agctaaactt
    10321 ttacatgtta ttaaaaccaa attgatgttc aatttgtaca ctgagtgagt atgttaaacc
    10381 tgattaaggg ggagtcatct gtggaagatg tgaaaaaagt attattaaca aaatcaaaac
    10441 gttcaaaaat ggaacagcgt ggaaaattca tcagtttttt atgtatcgcg ctgatcgtgt
    10501 tggttgtatt atctattttt tattttgtag ctagtaaagg attggccacc ttttttactg
    10561 ataagatcaa tgtatttgat tttctgtttg gtacagattg gaatccaagc caaatcggag
    10621 caaatgggaa accaatggta ggggcattgc ctatgatcac aggttcgttt attgtgacct
    10681 ttttatctgc tattgttgcc acaccatttg ccattggtgc agcagtattc atgacggaga
    10741 tctctcctaa aaacggaacg aagatcttac agccggtcat cgagctgctt gtaggtattc
    10801 cttctgtcgt ttatggtttt atcggtttat cagttatcgt gccttttgtc agaagtattt
    10861 ttggcgggac agggtttggt atcttagctg gtacatttgt gttgttcgtt atgatcttgc
    10921 cgactgtcac aacgatgact gttgatgcat taaaggcagt accacggcac tatcgggaag
    10981 cttcattagc gcttggtgcc acaagatggc aaacaatcta caaagtcgtg ctacgagcag
    11041 ccgtgcccgg gattttaaca gcagtcgttt ttggtatggc tcgtgcgttt ggtgaagcct
    11101 tagctatcca aatggtcatc ggtaatgcag cattgatgcc aacaggactt actactcctg
    11161 cttctacatt gacgtccatc ttaacaatgg gaatcggaaa tacaatcatg ggaacagttg
    11221 aaaataatgt gttatggtca ttagcgctga tcttgctatt gatgtcatta ttctttaata
    11281 tcgtgatccg tatgattggg aagaaaggag agctgaaata atgaacgcaa aacgctcaga
    11341 taaaattgct acaggtgttt tatatgtagt ttcggggatc atcgtattga ttttagcagc
    11401 tttactactt tatatcttag ttcggggaat accgcacatt tcatggtcct tcttgaccga
    11461 accttccaaa gcctaccaag aaggcggtgg aatcgggatc cagctattta actcgattta
    11521 tttgcttttg atcacgatga tcatcagttt tcctatttct ttaggtgcgg gtatctatct
    11581 ttcagaatat gctggaaaaa actggctgac agatgtgatt cgcacgtcga ttgaaatctt
    11641 gagctcattg ccttctgtcg ttgtcggttt attcggtttc ttggttttcg ttattcaatt
    11701 ccagtatggt ttctcgatta tttctggtgc attggctttg actgtattca acttgcctct
    11761 tttgactaga acagttgagg agtctttaca agcggtccat tacacacaac gagaagcagg
    11821 attggcttta ggtttgtcac gctgggaaac agtgataaaa gtggtggttc cagaagcgac
    11881 accagggatt ttgactggtg tgatcctaag ttctggaaga atttttggtg aagcagctgc
    11941 attgatctat acggctggac aaagtgcacc tgcgttagat tttacgaact ggaatccatt
    12001 aagtgtttca agcccgatca gcattttccg tcaagctgaa acattagctg tccatatttg
    12061 gaagatcaat acagaaggca cgatgcctga tggtgtttct gtttcggctg gcgcttctgc
    12121 cgtgttgatc attgctgttt tactgttcaa cttcggcgct cgtgcgattg gcaaacgatt
    12181 ataccgaaaa atgacttcag cctaaggaga agaagcaaaa tgaaaaaata taactggaat
    12241 gaaacaaatg tcatcaccat gccaaaagag aaagaagtag cactatatac ggatgatcta
    12301 catgtctggt atggcgacaa tgaagcaatc aaaggagtgg atctggaatt tgaaaaaaat
    12361 aagatcactg cactgattgg tccttccggc tgcggaaaat ctacgtatct tcgttcgttg
    12421 aatcgaatga atgatgggat cgctaatacg aaaatcactg ggaaaatcat gtatcaaggc
    12481 gtagatatta atgcgcctca agtggatgtt tatgagatgc gcaaacggat tggcatggtt
    12541 tttcaacgtc ctaatccgtt cagcaagtcg atttatgaca atattacatt tgctttgaaa
    12601 cagcacggcg aaaaaaacaa aaagaaatta gatgaaatcg ttgaaacaag cttgaaacaa
    12661 gctgccttgt gggaccaagt aaaagacagc ttggacaaaa gtgccttggc tttatctggc
    12721 gggcaacagc aacgtttatg tattgctcgt gcgatcgcga tgaaacctga tatcttgctg
    12781 ctagacgagc cggcaagtgc tttggaccct atttcgacag gaacggttga agaaacactt
    12841 gtaaacttga aagaagacta tacgatcatc atcgtgacgc ataacatgca acaagcggct
    12901 cgtatcagcg actatactgc ctttttctat ctaggaaaag taatggaata tgacaaaact
    12961 aacaagatat ttaccagacc aaaaattcag gcaaccgaag attatgtgtc cggtcatttt
    13021 ggttaagacg gaggcctcaa catgacaaaa gaaattattt catctaaaga tcttcatctg
    13081 tattatggaa agaaagaagc actaaaaggg atcgatctaa gtttcaatca aggggaaatc
    13141 actgcgatga tcggtccttc tggttgcggg aaatccactt atctgcgttc tttgaaccgg
    13201 atgaatgacc tgattccgaa tgtaacgatc accgggagtg ttttatataa agacaaagac
    13261 atctatggtc caaaaacaga tgttgttgaa ctaagaaaag aaatcggcat ggtattccag
    13321 cagccaaacc catttccttt ttctatctat gaaaatgtca tctatggatt gaaattaaaa
    13381 ggggaaaaag ataaaaaagt ccttgatcaa gtagttgaag aaagcctgaa ggcagcgtct
    13441 gtttgggatg atgtgaaaga caaattgcat aaaagtgctt tatctttatc cggtgggcag
    13501 cagcagcgtg tatgtatcgc acgtgttttg gcagttaatc cggaaatcat cttattggat
    13561 gaaccaacaa gtgcgcttga tcctgtttct actgggaaaa tcgaaagtat gctgttagaa
    13621 ttaagagagc ggtatacgat gatcatcgtg acccataata tgtctcaagc ttcacgtatt
    13681 tcagataaga cggcattctt tttagatggt cacttgatcg aatacaatga tacgaagaag
    13741 atattcatga atccagaaaa acaagagaca gaagattata tttcaggtcg atttggataa
    13801 ggaggaaacg gaatgctacg tacacaattt gaagaagaat tgctgaatct ccataatcag
    13861 ttctacgaaa tggggatgat ggtgagcagc gccgtgcaca agtcagttcg tgcatacatc
    13921 aaacacgata aaatcttagc ccaagaagtt atcgaaaacg atataaatat caataacatg
    13981 gaaacacgct tggagaagaa aagttttgaa atgatcgcat tgcaacagcc agttacaact
    14041 gatttgcgta tgatcattac ggtcatgaaa gctagctcgg atttagaacg tatggccgat
    14101 catgcggtgt ctgttgctaa atccacgatt cgcgtaaaag ggcagacgag aataccggaa
    14161 atcgaaaaag agatctccga tatgtccgat tatgtgaaaa aaatggtgga caacgtccta
    14221 gttgcttatg tcaaaacaga cgaaaaagac gcacgtacga tcgccaatat ggatcaaaga
    14281 gtcaatgact atttcaatcg aatctataat gcaacaatca aaaatatgca ggaaaatcct
    14341 gagacagtca tcagtggaac agactacttg aatgttgcca gttatctaga acggatcgga
    14401 gactatgtga caaacatctg tgaatggatc gtctacttgg ctacagggaa aatcactgag
    14461 ttaaatacga accacaatga gattttctaa actaaagcgg tgattctgcc gtttgtttag
    14521 gaaaagaggt tgtgaaatca gcggttgtga aaaagctgtt ctgcatcaag taataagaac
    14581 agtcaaacaa aaatagctcc tcatattttt catttggctg gacttattac cgcagatgca
    14641 agcttttgaa cactgtttat tcgggaaagg gtttgtgaca aaatatgtca caaacccttt
    14701 tcatcatttc atagaaagga taaagaatgc aaaaaaaaat tagcatcatc actatatttt
    14761 ttatttgtgt gcttacttca tgggttattc agcatcaagc tgctttatat gaagtacctg
    14821 tcatcgagat cgaaaaagta cgaataaaag aggatcatca attgattaca ggaagactga
    14881 tgaacacgaa tcaaaaagga gaatcgattt atttatcggt tccttatcaa aaaaatcaaa
    14941 tagaaacagc aaatataaaa gcgagacaaa tgtatctagt ccaaatgcat ggagatgaat
    15001 ggcagctagt agcaaaaaaa aatgatggct ggcttttctt cttcacaagt atcttcattg
    15061 gcgccatgtt gcttattggc ggaaaacatg gtctatttgc tttaggaagt ctcatcatca
    15121 atatcgtttt acttttactt ttgttgctca tctttactaa aatagaaggg atttctctat
    15181 taaatatatc tatcctgttc gtcgttatag ggatgattat tagcatattg gctttagatg
    15241 gatggaatag aagcagtata atcaaaattt cagctgcagt tacgagtgtt tttttagcat
    15301 tttttatttg tcttttgaca atgaatcttt ggaaagacca aggactgcgc tatgaggaac
    15361 tgaattatct gacacgaccg tatcgttctg tttttctttc tagcgtactg attggaacag
    15421 ctggaggaac actcgatacc gttatcacag tcattgcgac attagaagaa ttgactaagc
    15481 gacaaccaga aataacagcg aaacagttat ggctttcggg aaggcaagtg ggtcgagatg
    15541 tgattggctc gatgaccaat atccttttgt tcgtctatat cagtgggtca gttccatcgc
    15601 ttcttttata tttaggaaat caatggtcgt tcaaagaaac gatagaacag catttatcat
    15661 tggaattttt acgcgtcatt gctggcagct taggaatagt attatcgatc cctatctcgg
    15721 tcctcttttt tctgactgta aggaggctga aacgatgaat gtacttatta tacttgttgg
    15781 gattttagca atcagcgtgc tacttattgg cggtacgcaa ggtatgtata ttcttttagg
    15841 cttattcatc aatttaggga tctttttctt gttgttattc ggttatcatc agaagtggcc
    15901 gatactggca ttgtcggtca taggattttt attgattgct gtggttatct tattttttat
    15961 caacggttat aatctaaaaa tgcgtgcagc ttttgtgagt atactgatct ttttattttg
    16021 tttcctacta ttgcttccta tcactgattt tttagcaatc caaggtttta caacgataga
    16081 actagaagaa ttatctggct tagataagac tttagcgatt gattttagat tacttacgag
    16141 atcgttgctt ctgattagtc ttagtggtgc tgttttagat gcgagtgtag caatttctag
    16201 tgggacattc gaagtatatc aggcaaatcc gcaattatct ttttaccaat tgcgccattc
    16261 tagtttcgct attgctaaaa aaatattggc gtcaacagta atgacacttc tttttgcatt
    16321 catgggaagt tctttagcgt tgatcctctg gtttattgat ttagacattc cttttcagca
    16381 agtgatcaat gaaaaatcat ttgttctaga atatacgatg gctattctta ccacattgtc
    16441 cgcactactt gttcttcctt taacctgcgt gactacagca tatctcttta caaaaaagaa
    16501 agaacaactc aagatggagt aaaagttaca acttaagtcc tatgacattt ttactgaatc
    16561 agtgcataat atggatgtaa ggaaaagctt gcaaacaatt ttgcttaagg aggcaatttc
    16621 catgaaagaa agagaacgta ttttagattt agtgaaaaaa ggaatccttt cgactgaaga
    16681 aggcttagat ttattagaaa gcatggcaac tgaaaaagac gaaaagcaaa taaaaaaaga
    16741 agcagacaag gtaactgcta gtcataaaga aaaagatcag gcaagtcaat tgatcgataa
    16801 acttgaaaac ggcgaagaag aaatttctga gccagttgat ccaaaggaaa gagaaagaca
    16861 agatcaagaa aacttagaaa agatcttaga tgaactagca actgaagcga ataaaacttc
    16921 ggcgcgtcta gatgaagtaa atgctcaatt cgctgacaat aaggcagcac gcaatgagaa
    16981 acaggaagca ttgatgcagc tgaatacaaa agaagaatta ggcgagttga cagaagaaga
    17041 attagcacag cgtcagacgt tagaagcaga aatcaaagag ttagaagcta gtggcgatac
    17101 actgttagaa gaacaattga aattagaagc agaattaaaa gatatccgta agaatcaatg
    17161 gtctgagaaa aaagaaacgt ttactgataa gtttgaattg ccagatgatt ggaaagatca
    17221 agcaacagat acactgaatc aagtaggcga aaaaatgtca gaagctggat cacagttagg
    17281 aaaattcttg aagaaaacat tccaaactgt ttcagaaaca gtgaatgata acatggaatg
    17341 gaaagatgtc agcttacgtg tccctgggat tgcgacaacg aaatttgagc atgaatttta
    17401 ctatgaagct cctgcagcaa gtatccttga tatcaaggca gcaaacggaa acgtgacgtt
    17461 aaaaacgtgg gacagcaacg atgtgaaagt tgaagctaag atcaaacttt acggaaaaat
    17521 gggtgctgaa ccatttgaag cattctctga acggagccag attgaggtta atgaagatca
    17581 tatctccttc caaattccaa ataaacgtgt gcgtgcagac cttgttttct acttacctaa
    17641 acgagtatac gaccacgcag ctattaaact tctaaatggc aacatcatga ttgaaacatt
    17701 ggaagctaaa gatatttata caaaatctac gaatggtaac atcatcgtgg accaattaac
    17761 tgcaacgatg ctggaagtag aaggtgtaaa cggcaatatc gatattcgaa acgggaacat
    17821 cttagattct attatcgaaa cagtaaatgg tacagtgaca tttggtgcga cagcagaaaa
    17881 tctttctgtg tctttagtaa atggtgatgt acgtcttacg attaaagaag ataatttgaa
    17941 aaaagtagaa gcaagttcag tgaacggaaa tgtcaaagtc gctttaccag atggcatcgg
    18001 tttggaagga catgctaaaa caagtcttgg aagtatcaat agccgtctgt ccaattacga
    18061 agtagttcgt gagaaaaaag aacggacaaa ccaaatgttg caattccgtc gtgtttcaga
    18121 tggtgaaatc gcgcaagtgc aactttcaac gacaacagga agtatctatt taaaagatac
    18181 agataaataa agaggttgtg aaagagatgt tcagccttga gcaataagaa gcgattttgt
    18241 aaaatagctt cccctatttt acaaaattgt gccttattgc cgataggcta tctcttgaac
    18301 accgtttacc aagagattat gaacttgcca cttatcgccg agtcacaagg aataatttca
    18361 aaaaaagctc gtcacgtttt atgaatatgg taatttgtga ccgagaattt tgcagagaga
    18421 caccgattaa gaagaaggtt gtcacaacga ttttcttcat ttaaaatagt agaacagagg
    18481 tgagacatga tgaaaaaaaa gttgacaaaa tccaataaaa atgttgtttt gacagggact
    18541 ttagcaggaa taggagaata catcgggatt gatccgactg ttatccgtgt gctttatgta
    18601 ttcctgagct tagttgcttt tggaagtcca atcattttat acattttatt agctttgatt
    18661 atcccatctg cgcgttctgc acgaacaaac tatggacata acaatgcata ttatgaaaaa
    18721 aacaattatc gtgaacaaac tcgtgaacaa acaaaaaaac gcaaagaagc ggaaaaaatc
    18781 gatgatgacg aatggagtga cttttaatgt cgtactttca acgtatttta gttaatacgt
    18841 taacattcat atcattggct gttctttttc ctgcgatggt ccatgtaagc agtatttgga
    18901 cggccgttct tgccagcttc gtattgtctg tgttgaatat gtttgtcaaa ccgattttga
    18961 cgatactgtc cctgcccttt acattagtca cattgggatt gtttacgttt gttatcaatg
    19021 cagcgattct taaaatgacc tccttttttg tgggagcaga acattttagt ttttcttcat
    19081 tcggtgcagc gttgttagta gctatcttga tgtcgcttgt caatgtaatt gtcagcgaac
    19141 ataacagtag aaaataaaag aaagctgtgc cgaactatcg gcgcagtttt tttatttaga
    19201 atcaggaatg cgttttctat tacattggta attaaataaa aacgtttttt ttaattggta
    19261 gtacttttgg ctagacaatg ataaaatgat agagaacact ctaatagaaa tgaggtagca
    19321 tgaatgtcag aagttgtaaa agttagccaa ttagtgaaaa aattatcttt ggaaatcgtg
    19381 acaggcgacg agaaaagctt agaccgtgtc atcgtaactg gggacatctc acgcccagga
    19441 ctagagttga ctggttattt taattattat tcccataatc gtttacagct atttggcagt
    19501 aaagaaatca gttttgcgga acggatgatc ccagaagagc gtttgatcat catgagaaga
    19561 ttatgtagtg aagatatgcc cgcatttatt atttcaagag gattaccggc tccagaagaa
    19621 atgatccagg cagcaaatga aaatgggata gctgtactgc gttctcccat ctcaacttct
    19681 cgtttattag gagaattatc cagttattta gatggtcgcc ttgcccctcg aacaagtgtc
    19741 cacggtgttt tagtggatgt ttacggatta ggtgtactga ttcaaggtga cagtgggata
    19801 ggaaaaagtg aaacagcgtt ggagttgatc aaacgcggtc atcgattgat cgcagacgat
    19861 cgagtagatg tttatcagca agatgaatta acggtgatcg gagaaccgcc taaaatcctg
    19921 gaacatctaa ttgaaatccg aggaatcggg atcattgatg taatgaatct ttttggtgcg
    19981 agtgcggtac gtggctctat gcaagtccag ttggctgttt atctggaagc atgggcaaaa
    20041 gataagaaat acgaccgtct gggctctgaa gacacgaccg ttgaaatcgc ggaagtgggc
    20101 attccacaaa taaaaatccc tgtcaaaaca ggacgaaacg ttgcaatcat cattgaagta
    20161 gccgcgatga atttccgtgc acgtacaatg ggctttgacg caacgaaaac atttgaagaa
    20221 agattaagtc gcttgattga agaaaactca ggcaacgact agcaaggagt gacaatatgt
    20281 tagcacaaat caatcgagtt gcttttgagc ttgtagggat tcctatctac tggtatgcat
    20341 tgatcatcgt atcaggtatc attatcgcca tgtggcttag cagcagagag gctgttcggg
    20401 taggattaaa agcagatgat gtgacagatt ttatgttgtg gggattgcct atctccatta
    20461 ttggcgctcg cttatattat gttttatttg atcttcccca gtatatagct gatccaatcc
    20521 aaatattcaa tatccgttca ggaggcttag ctatttatgg cggactgatt gcaggcgcga
    20581 tcacgctgta tttttttact aaatatcatt ttatttcttt gtggactttt ttagatatcg
    20641 ctgcaccaag tgtattatta gcacaggcaa tcggtcgttg gggaaatttc atgaaccacg
    20701 aagcatatgg accagacacg acgcgaagct tcttagaaag tctgcattta ccgcaattca
    20761 tcattgataa tatgtatatc gaagggatct atcgtcaacc aacatttctt tatgaatcac
    20821 tttggagttt gattggattt attttgcttt tgtttttacg aaagaaacca caccttttga
    20881 aaaaaggcga agtcctttta tgttatctaa tgtggtatag tttcgggcgt ttctttattg
    20941 aaggattacg tacagacagt ctttactttt taggtgtgat ccgtatttct caattattat
    21001 ctgctgtgtt atttgtcggt acattgattc tatttatttg gcgcagaaaa aaaggaaatt
    21061 taccggatta tgaccgctca ttaggaaaaa atcaacaaat tatctgattt ttagagcgtt
    21121 aaaaatgaaa aaaatttcag gagtggtaaa atgaattata gagtagcggt tttaggacca
    21181 ggctcttggg gtactgcttt ggcacaagtc ctcgccgaaa acggacatga cgttcggatt
    21241 tggggacatc gtgcttcaca agtagaagaa atcaacaccc aacacacgaa tcaacgttat
    21301 ttaccagatc attatctacc cgattccatc caagcattca ccaatatgga agatgcagta
    21361 aaagacgcag atgcggtgct attcgtcatt ccaacgaaag caatacgttc tgttgcaaaa
    21421 gaactagtgc ctaaattgaa tacaaagcca gtgatcatcc atgcaagtaa aggattagaa
    21481 cagggaacac ataaaaggat ctctgacatc ttgttagagg aaatcccagc agaaaaaaga
    21541 aaagctgttg ttgttctttc tggaccaagc catgcagaag aagtagcaaa acatgacatc
    21601 actacgatca cagctgcaag tacagatgca aaagcagcaa aatatgtaca agagctgttc
    21661 atgaataatt attttcggat ctataccaat cctgatgtga tcggtgtaga gatgggggca
    21721 gctttgaaaa atattattgc tattggtgct ggcgctattc acggcttagg gtttggcgat
    21781 aatgcaaagg cagcaatcat gacgcgcgga ttagctgaga tcagccgttt aggtgtagca
    21841 atgggtgcca acccgttgac ctttatcgga ttaagcggag taggcgatct agtcgttact
    21901 tgcacaagtg ttcattctag gaactggcgc gcaggcaact tgttaggaca agggcagccc
    21961 ctagaagagg tgctagccaa tatgggaatg gtcgttgaag gtgtgaatac tactcaagca
    22021 gcgatggaac tagcccaaat aatggaaatc gaaatgccca tcactcaaac aatctatgaa
    22081 gtgctgtatg aacaaaaaga aatcaaaaca gccgcaaaag aaatcatgtt acgtgatggg
    22141 aaaaaagaaa atgaattctc attaacaatt ttttgaagga gcgaatgtat atgaaagtca
    22201 aaaaagcagt tattccagca gcaggattag gaacaagatt tttaccagca acaaaagcta
    22261 tggcaaagga aatgttgcca attgttgata aaccaacaat ccagttcatc gttgaagaag
    22321 cgttgaaatc aggaatcgaa gacatcttga tcgttacagg aaaagctaaa cgaccaatcg
    22381 aagatcactt tgattcaaat attgaattag aaacaaacct aagtgaaaaa ggaaagaccg
    22441 aattattgaa attagtagaa gaaacgacag atgtcaactt gcatttcatc cgtcaatctc
    22501 atccaaaggg tttaggacat gccgttcttc aagcaagagc ctttattgga aatgaaccgt
    22561 ttgtcgttat gctaggcgat gatatcatgg aagatgaagt accattgaca cagcaattaa
    22621 tcgatgatta cgaacgtact catgcttcta cgatcgccgt tatgcaagtt cctcatgatg
    22681 aaacttcaaa atacggaatt atcaatccag gagaagtatt ggaagatggg ttatacaatg
    22741 tcaaaaactt tgttgaaaaa ccagatccaa gcaaagcacc aagtgattta gctattattg
    22801 gacgttactt attgactcca gaaatttttg atgtattgtc cgagcaacaa ccaggagccg
    22861 gaaatgaaat ccaactaact gacgcaatcg atacattgaa taaaacacaa cgcgtatttg
    22921 cccgtaaatt cacaggtaaa cgttacgatg tcggtgacaa atttggtttt atgaaaacaa
    22981 gtattgaata tggtctaaca catccagaag taggtgcacc tttacgtgag tatatcctgt
    23041 ctttaggtaa aacattagaa gcagaaggaa gcaagatcga tcataatcca aaaggaataa
    23101 cagaaatcaa agaagaaaag aaaaaataac ccttttttct aacgagcttc agctgctaat
    23161 tgaataaaaa gtagttgaag ctcgttttta tttgtttaaa aaacacgaaa atgctatcct
    23221 tatagaaaag agagacaaga agggagtttg atcatgtcag gaggagaaat tgcaggatta
    23281 atcgcagcaa tcgcctttgc gatacttgtc gtttttatcg ttcgtgtgtt gttgcaagtt
    23341 tctaaaactt tagaaaaagt agatcagact gtagcagaag caaatacgac gatcgaaatc
    23401 gtcacaaaag acgtagatct tttgtctaga caagtagaag gcttgctggt caaaagtaat
    23461 gaactgctct tggatatcaa tggaaaagtc gcaacgatcg atccattgtt cacagcagtt
    23521 gctgatttaa gtgaaagtgt atcagaactt aatcattcta gtaaaaacat cgcgacgaaa
    23581 gtcggcggaa tcggtaaaac gacagctaaa gcgacggttg ccggaaaagt cggtgggaca
    23641 gcgatgaaat tttttaaaaa caaaaaacat actgaaacga caggaggaaa ataaaatggc
    23701 taaaaaaggt ggatttttat taggtgcagt agtcggagga acagccgcag caatcgccgc
    23761 gttattgtta gcaccaaaat ctggtaaaga acttcgggat gaattagctg cccaagcgga
    23821 tgatttgatg gatatggctt cagattacac agatatggcg aaagaaaaat cgactgagtt
    23881 aacaggtatt gcaaaagaaa aagcgcaaga tctttctcaa caagcaggtg atctagcagg
    23941 tagtgtcaaa caaaaagtaa gtgaatcagc agatgaagca ggaagtgaat ctgacgatat
    24001 cttgttaaat cttaaagaac aatcagctga cttatctaat cgtttcaaaa aaacggcaga
    24061 agatatgaaa aaacctgcag atgaattagg aagtattgct aaagatactt cagaagatat
    24121 catgattgat gtaaaagacg ctgctggtaa agcaggagat actgtcaatg aaggaatgtc
    24181 agaagcaaaa ccagttgcag atgacacaaa agaaatgatg aaaatggcaa aagaagatgc
    24241 gaaagaagct aaaaaagaaa taaaagatac aatgacagat tcatctacaa atcactaaaa
    24301 tcacaaattt atagaataaa aaggtgggag cggactagct ctcacctttt ttactcttat
    24361 ttttctaaaa caagtaattc ttttggtgta gtcgtgaatg gcacacagcc atcgcttgtc
    24421 acatatacac agtcttcgat acgtacccct actttttcag gaatatagat tcctggttca
    24481 agagagaagc acatcccttc ttcgattact aagtcatttc cttctacaag ggaagggaat
    24541 tcgtggacgg ttgttccgat cccgtgacct aaacggtgat tgaaatattc gccgtagcca
    24601 tagctgttga tcacgtttcg tgcgatttga tcaagttcac ctgctgtcac acctggacgg
    24661 acggcttctt gtgcggcaag ctgtgcttca agagtgatgt tgtatatttt ttcttggaag
    24721 tcggttggtt tttgataagc aattgtacga gtagcatcac tgcaatagcc attccaaaca
    24781 acgccaagat cgaataacac aagttcattc ggtaaaatct tcgtgtttcc tggtgtacca
    24841 tgagggcttg cagcatttgc tcctgcaagg actaatgtgt cgaaagacat ttgtgaaaca
    24901 ccttgttttt tcaattggta ttcgatttct gcaatgatcg cttgttcagt cacgccttct
    24961 ttcactgctt taaagccaat ctcaaatgca acatcggccc atttaccagc ttcgatcagt
    25021 ttgtcttttt cctctagagt tttgatcagt tgtagacgtt ggatgatgga agttagatca
    25081 gtgtaagaat ccgttcccgt caagatatca gagatagcat cgaagcgctc gacagtcaag
    25141 gcgttctttt caatccctac attgccaggc tgaccatagc gagcacgaag ttcttttccg
    25201 atgatctccc aagggttttc gctgtctaaa tagccacgga catcatacgc ccaactactg
    25261 ttttgagcat cttcaacttc caaagcagga gtgaaaagga aaggatcttg ttcaagaggg
    25321 ataaaaagag ctagtacacg ctcatgagga tcactttcgt atccggaaaa gtaagcgatg
    25381 tggccagggt cgctgatata agccaattcg acttggtgtt gtgccatcca ttgacgtagt
    25441 tcattgattt ttacgttatt catttggcaa cctactttct aaattaattt tgtattcaat
    25501 aattgtacca ctgatttatt aaaagaccaa cattctaaaa actgaaaaaa gtaaaaattt
    25561 tcatgaaaac agagataaaa cggttgcaat tcttgagatt atttgatatt ctattttaga
    25621 aatgtaagac gtattttcag aaaacatata aaatatgaat aggagaacac aaatggaaaa
    25681 gcaaacaatc actatctatg atgtagcgag agaggcaaat gtttcgatgg ctactgtttc
    25741 acgtgtcgta aatggcaatc caaacgttaa accagcaaca agaaaaaaag tcttggaagt
    25801 tatcgatcgc ttggattatc gtccaaacgc agttgcgcgt ggcttggcaa gtaagaaaac
    25861 aacaacagtt ggtgtaatca tccctgatgt cagcaatatg ttctttgctt ctttagcacg
    25921 tggtatcgat gatgtggcaa caatgtacaa atacaatatt atcttagcaa attcagatgg
    25981 caacgaccaa aaagaagtca atgtattgaa taacttactg gctaagcaag tggacggcgt
    26041 gatcttcatg ggtcaccata tcacagacga aatccgtgga gaattttcac gttcaaaaac
    26101 tcctgttgta ttagctggct ctattgatcc agatgaacaa gtaggtagcg taaacattga
    26161 ttatgtatcc gcaacaaaag atgcagtaaa caaattggca aaaagcggca ataaaaaaat
    26221 cgcttttgtc agtggtgcgt tgatcgatcc catcaatgga caaaatcgtc tgaaaggcta
    26281 taaagaagca ttaagcgaaa ataacctacc atatagcgaa ggattgatct ttgaagcaca
    26341 atacaacttc aaagatggtc tatctctagt cgatcggatc cataatagcg gtgcaacagc
    26401 tgtctatgtt tcagatgatg aattagccat cggtattttg gatggtttat tagaccgtgg
    26461 cgtgaaagta ccggaagaat tcgaaatcat cacaagtaac aattcactac tgacagaagt
    26521 agctcgtcct cgtttaacaa gtatcacaca accattatac gatttagggg cggtatctat
    26581 gcgcctattg acgaaaatga tgaataaaga agaagtcgaa gaaaaaacga ttatcttgcc
    26641 ttatggaatc gaagaaaaag gatctacaaa ataataaaag gacaaccatt cagaggttgt
    26701 gtgcctgccg tttaactctg cgttacaagg agcaatttca aaaaacagca tctcatgttt
    26761 gatgaatatt gtgactcgtg attcggagtt ggcaggagaa caccgtttat ttgggaaaag
    26821 gggttatgac aaaattttgt tataatcctt tttttgtgtc ttaaaaaaga gagaaagtgg
    26881 ttgaaattat atttatcgta tataaaacaa tccgtgtata gatgctgtat tttgttcacc
    26941 atcatcaaaa aaataaggga atgtgacaat actattgtca cattccctta ttactgtgta
    27001 ttctattaaa tcaatcatct ttctcatctg attctttggt tgcttgattc gctcttgcat
    27061 aagctgaagc ggtgttccag taatctgtat agttttcatc ggtaccgcca acgccaaact
    27121 taaaggcgct tttcttcggt ccatttttcg cccatagcga ttcaactgtt tttcctggtg
    27181 tttggatgga ttttttattg acagtaactt ttccaggtgt ttgaccagta aatccagata
    27241 cttttttctt catcacatca gatgataatt cgaatttctc atcagtgcca aaaatgtcag
    27301 gttttgcttg gtaagcttga ttgatcagat tggcaagata attagccgaa cgtatccttg
    27361 ctttgctgtc cattccagta ttgtcatcgt gccctgccca gctactgatg gtaatagacg
    27421 gagtagagac aattagccat gaatctcggt attcattggt agaaccagtt tttccgaccc
    27481 aatctgcttt tcctagattt ccgtttaagc ttgagatggc atctttgaat ggtgtagtga
    27541 tttttgcatt gatcacactg cgcatcatgt cattcatgat cgatgccgtt gcttccgaat
    27601 agatccggac agagttgctt tcgtgtttat aaattacatt tcctgcgtta tctgtgatgg
    27661 aatcgatgat atagccttct tcatagacgc cgccgtttgc taaagcctga aatccgttgg
    27721 tctgttgaag cgtcgtgaca tctactgtac ctaaaggagc agattcaacg ccccaattgt
    27781 catttgccgg gtagttcatc tttgacaagt agtgctcgta agcatattgt ttcgatccac
    27841 cggaattcaa cacatcttga tataagtgat aagccgggat attgttggac cattctagtg
    27901 actcacggac ggtttggaag gtgttcgatc cttcgttagt agcattgacg attttctcgc
    27961 cagcattttc tccttcttgc caagttgttg cataatctga tacacgggat tctgatccga
    28021 taagccccat gtcgattgca ggtccgtaaa caagaactgg tttgattgat gagccagctt
    28081 gacgttctgt atcgaaggca tggttatttt gattctgact gtaattccgt ccgccaacga
    28141 atccgtagat gcggccagtt ctgttatcca tcaatacatt tccggtttct acttgactgc
    28201 cagcaccatc atctaacatg tatccataat tttcaacgcc attttgcaat gcagcataga
    28261 catctttatc gattgtcgaa tggattgtat atcctttatt ttggatcgtt tgctgtgcct
    28321 tttgataata agcgtcactg atcgatgaat cagacatatc agcgttgtct ttttcagcca
    28381 attgttgtgc aatgattctt gttgcttcat tcatcacagt ataatacaag aattctctgt
    28441 cagtctgttc tgcgatttgt tgtggtagaa agtcttttgt aaggtcatag gcttttgctt
    28501 cttcgtattg ttctttcgta atttggcctt cacgatacat actgaataaa acaaaatctt
    28561 ttctggcaag gccggcagaa agatcttctt ttagcgcacc tgtgttcgtg tatggactat
    28621 acgtgatcgg gctttggggt aatccagcga tataagcagc ttgcggcaga gtaacatctt
    28681 ttgcatctac accaaagatt cctcttgcag cttcctggac tccagcaatg ttttgtccct
    28741 tattattacg accaaatggt gagacattca aatacgtagc aatgatctca tcttttgaaa
    28801 agtatttttc tacttgtgca gaaagaatga tctcgtttgc tttacgtttg aatgttgttt
    28861 catctgtaag gatctgctgt ttgactaatt gctgtgtcaa agttgatcca ccagaagagc
    28921 caataccagt agcttcagat actaatgctc gtaaaacagc tttaggaacg taaccttggt
    28981 gtttatcgaa gtattcatcc tctgtgctga taattgcagt tttcaataaa ggtgaaattt
    29041 tatcagatga aatagtcgtg cgcatcagat ctgtctggat ttttgatatc tctgtattat
    29101 ctgcataaac aatctttgac gtttctgtaa tgtttcctag ttctgtctgc agtgcttttt
    29161 tagtcggcaa ttgtgtgtct tcgacgagat aggcaaaata gccagcgcct attccaatac
    29221 ctagagctgc gaacatcaaa agaagaacag taataaaaac agtcaatgat tgaaaaactc
    29281 taaaaataat cttaggaaca aaccattttt ctttttttct tctattctgt ttttttcgag
    29341 tatttttgcg tggcaaaagg gtcacctcaa ctaatggttt tatttcgcta attatagcaa
    29401 ataatagaac aaaaaaacag acaaggctgt ttttggcagg aatttctttt ttaaaggagt
    29461 taaaacgtga gaaaatgatc ttttttataa aagagttctt aagcttttct ttagagaaaa
    29521 tttcagttac tatggagaac agggcaaaga aaaatcaatc attttctttg tatatcatct
    29581 taccaatata taggaattgt tagtaacaat tgcctttcaa ggaggaatcc atccatgtta
    29641 ttaggctcac acgtcagcat gagcggaaag aaaatgctgt taggagcagc agcagaagca
    29701 gccagttatg gatcttcgac ttttatgatc tatacaggcg cccctcaaaa cactagaaga
    29761 aaagccatcg aagagatgaa tattgatgcc ggaaaagcgt acatggcaga aaacggtcta
    29821 acaaatatcg tcgttcatgc cccttatatc attaatttgg ggaatacgat caaaccagaa
    29881 atgtttcctt ttgccgtcca atttctgaga gaagagattg ttcgtgcgga agctttaggc
    29941 gctactcaaa ttacacttca cccaggtgct catgtagggg caggaacaga agccggaatt
    30001 gctagaatta ttgaaggctt gaatgaagtt ttgacaaaag aacagacggc acaaatcgct
    30061 ttggagacaa tggcaggtaa aggtacggaa atcggtcgaa catttgagga attagcgcaa
    30121 atcatcgatg gagtaaaatt aaatgaaaaa ttatccgtta cttttgacac ctgtcatacg
    30181 aatgatgccg gttataatat taaagaagac ttttcaggtg taatggaaga gtttgataag
    30241 atcatcgggt tagatcggct taaagtactc catatcaatg attctaaaaa tccgcaaggt
    30301 tcgcataaag accggcacgc caatattggt tttggaacaa tcggattcga cacgctgaat
    30361 aagatcgtcc atgatccaat gtttgacaat atttcgaaaa tcttggaaac tccctatgtc
    30421 ggcaaagata aaaagaaagc ttacgcacca tatggaaaag aaatcgccat gttcaaagca
    30481 caaatattcg atccagaagt gtttcctgag ttattagcag aagaagtgat actctaagat
    30541 tttctatctg acagcagcaa aaaaggacca ggcacattgc cttgtccttt tttgtttaag
    30601 aaacaaagga gtactttttg cagtcttttt catcatgcat cttctccccc ctctttttaa
    30661 atttagacaa acaaggtata ctgttagaaa agtaaaaaaa cgaaaagttt cacaagcaag
    30721 tggcaaaacg tgattggaag gacgaaaaga tgctgaacac tttaaaaccc ttgatcaacc
    30781 aactaaaaaa gaaacataca tactttattc ttggcatagc cctattattt acaattattc
    30841 gtttgtattt atatagtaaa gcaatctatg gaacagacat gtcggcaacc tatgatgacc
    30901 agctgctgat tcgctatgct caaaatattt tggacggtaa atggctaggg gagtatacga
    30961 ctacaacact ttccaaagga atctcctaca gcttattttt agtacttgca cataaactgc
    31021 atctttctta tgctgttttg ttcggcatgt tcagtatttt tgccagtata ttggttgcac
    31081 tcgcgtttaa accgatcgtg aagaacaagt atcttctttt aagtatctat ttttattttt
    31141 tgttttctcc gattagtttt acacatgaat attccactcg aacatatcgt aatgccctcg
    31201 tgattccggc tgttgagatc attatcgctt gtttactagc tatttattac cgcaaatacg
    31261 gttctagcag aaaattgctg ccatggtttt tagggctttc tgtcgctttt cctttctttt
    31321 ggttcattcg cgaagattct atctggcttc ttccttttac tgtagtagct ttgggtatca
    31381 gtttcgtcca gatcttgcta gggcaagact tttctttcaa agaagtaaag aagttaaaaa
    31441 aacgactctt tcttctctcg aaacgggaat ggctgaaact ttcattgttt ttacttccta
    31501 tagttggctt ttccatcgtc tatcagtgga tcgaatataa aaatgagaca acatacggta
    31561 ttgcaaccat caatgaccgt tccagtggag aatttggacg tttgatgaaa caactgatcc
    31621 gaatagatga tgaaacgaac ttgaataaag aaaacagtga gatatgggtc tcacatgctg
    31681 cactaaataa agcattagcc gcatctccta cattccatac tgtttctacc cgtattgacg
    31741 aattatatac gactcacgcg tggactcaag gtggaaaagt aaaagaatta cccggagata
    31801 tcatcttttg ggcattgcga gatgtggtaa gcgaatctgg ttattaccaa aacaatgcaa
    31861 aagaaaccaa tgatttttgg aaaaaagtca accaagaatt agaagaagcc tatcacaagg
    31921 gaaccatcaa aaagaaaaaa gagttctatc tgatgggatc aggagatgga aagacgatga
    31981 gtgatctgcc aatcgtattc gatttccttc aatcaggata cgcttatgca tggcgatata
    32041 aagatttcca tcaaggagga aattatagtt tcggaacgca agaacaagtc aaacaggctc
    32101 aagaattatt aaaccttccg ctaatggaga attggatcga tagtaatgaa ccgagaacac
    32161 ctaatttcca attaacaaaa tcagcaaaag tcgcgaatgt catcataaaa atttaccaag
    32221 ttctggctat tcccgtgctg attgcttcaa tagcagggat attacttatg atactaggaa
    32281 ccgtatttgc aaaagctgat cgtgcctatt atagttcctc tttgattgtg gtcaccggat
    32341 taatactgac acaattggtt tttcttttcg gtgtctcgtg gttttgttct tatgcgccag
    32401 aaaaaaagga ttatttttta agtgtctaca ctggcgcagg cgtgccatta acgcaattca
    32461 tcgtagtaac aagcatgcta gttttcagta aattaaatct gaaaaaggct ttatcttcta
    32521 aataaaaaga cccttggtag ctagattttt aacctaacta tcaagggtct ttgtgtggaa
    32581 aaatcagttt ctattcgatt tcttctaccg tttcctgcaa acgctgttca tattttttgt
    32641 ttcctttata taaatcacca atcgtccatg ccaacaggag aaggacaaga acaatgatta
    32701 cataagcgat gtgatcagcc aatgctagtt gagcagaagt ggcttgttca ccaaagaaac
    32761 tttcgatctg tcctggtaat cctcgtaagt tcaagtaagt tagactaatc acagagaacc
    32821 agccaagaag ttttacgatg aatgaatttt taaatcggct acccatctcc gcttggctgt
    32881 tggtcatcat cagtaaaggg atcatcgaaa acggcaacgc aaaagccaag aacacctggg
    32941 attcattcat caagttattc aaagcggtat gttcttcgat cgttcctttt gtacttgtaa
    33001 acaaaacaca aatcaataca ggaatcacag aaagcaatcg tgtgaccagt cttcttagcc
    33061 aaatcgggat acgcatatgg atgaagcctt ccatgatgac ttgaccggtc aacgtaccag
    33121 taatagtaga gttctggcca gaagcaagaa gtgctacagc aaataaagtg gataagatac
    33181 cagattttgc tacagcaatc aagataccat tgctcatagc tgatggatca gataaggctt
    33241 catacagacc aaaaaaggaa ggatcttgga cagaaccggt tttgaagaca gcgacaccca
    33301 tgatcaatag cagtgcattg actagaaaag ccatcgacaa ttggatatta gagtcccaag
    33361 tagaaaaacg tacagcttgt gctacatctt cttcgtcttg gtgatcaatc ttccgagttt
    33421 gagaaactgc tgaatgaaga tataagttat gcggcatgac tgttgcacca ataatcccta
    33481 aagctccagt cagcggagtt tcgccgccga tgaccggtga gtctgagaat gtatgactgt
    33541 tagggataaa gccagcaaat aaactgtgcc aatctgggtt agacaagcca acttgataga
    33601 caaaaacgaa taaaatgacg aaaattaagc agacaaccag tgcttcgatc ttacgaaacc
    33661 cgacttttgt cagtagtaag agaacgaaaa catctaatac agtaataaag acggcaatca
    33721 ccaatggaat atcaaaaagt aagtagaggg cgattgctgc accaatgact tctgcgatgt
    33781 ctgttgccat aatggctaat tcagtcaaga tccataaaat gacacccaat gtcttacttg
    33841 tgcgtgctcg gattgcttgg gccaaatcca tttgggtcac gattcctaat tttgctgcca
    33901 tatactgtaa aagcatagca atcagactag acatcaaaat aactgacatc aataaatatt
    33961 ggaaattttg tccaccagtg atcgaagtgg accagttacc tggatccata taaccaactg
    34021 ctactaatgc acctggtcca gagtaagcaa gaagtgtttt aaaaaaactt ttcccctttg
    34081 gtacatcaac tgttccattt acttcagcta aagataaacc atttgtatgt tcaataagtc
    34141 gatgcttatg aaattgtttg ttggattgat tattcaagaa aaaaccacct ttcctattta
    34201 tctacgatta cattctacac ctcaaaaaga aaaaattaaa gaaaaagatt cggtgtgcct
    34261 aatttatttt cgtggcgcgt cttgttagat gacgaaagaa attttgttct cctctaaaaa
    34321 aaccactatt ttttgcatga aactaaagaa tacgcctttc ttttttcaat aaactttagt
    34381 aaaataagtc attgagagca attcggaaat agaaaggaga gatttatatg ttatcaacag
    34441 gtattgtcgt acttattgct attattgctt tgctaatcgg tgcagtaggt ggctttttcc
    34501 tagcgcgtaa atatatgaaa gactatttag aaaaaaatcc tcccgttaat gaggacatgt
    34561 tgcgatcaat gatgatgtca atggggcaaa aaccatctga gaagaaaatt cgccagatga
    34621 tgcagcaaat gaagaaccaa gggaaaaaat aggttctgac ccaacgactt tttacgggct
    34681 gattttttca gcccgttttt tatttcgtaa tttgttagac tattcagatt attcagcaaa
    34741 aaagacaagc acagacaatc ctaggctctt gataaagaca aaggaggagt aaaatgtcga
    34801 tttttagaaa gttaggctgg ttttttcgtc aggaaaagaa acattattta attggggttt
    34861 tctcgctatt cgcagtggcc ttggtacagt tagttcctcc aaaagtgatt gggatcatca
    34921 ttgatgagat tgctgataaa aatttatcag taaagatcat tttcgggtgg atcttcctat
    34981 tgattatcgc tgctttttta caatatcttt ttcgttatat ctggcggacg aatatttggg
    35041 gaagtgcagc tcggttggag aaagaacttc ggcagcagtt attcaaacat tttacagaaa
    35101 tggacaatct cttttaccaa aaatatcgga ctggggattt gatggctcat gcaacgaatg
    35161 atttgaatgc gatccagaat gtagcaggag ctggtatctt gacctttgca gattcagtga
    35221 ttacgggcgg aacaacgatc attgcgatga ttttgtttat tgattggcgc ttaacactga
    35281 ttgcgctgat tcctctgcct ttgctggccg tcacttcgcg agtgctgggg tcaaaactcc
    35341 atgatgcttt tcgtgattcg caagaagctt tttctagtat caatgataag acacaagaaa
    35401 gtatcactgg gatcaaagtc attaaaacat ttggacaaga aaaagaagat ctagcggatt
    35461 ttgaagaaaa gattgaagat gcaattatta aaaacaaacg agtcaatttt ttagactcct
    35521 tatttgatcc attcattacc ttgattatcg gtctgtctta tgttgcaaca attattttag
    35581 gtggaaagta tgtcttggaa ggcacgatct cgatcgggca gatggtctca tttattagtt
    35641 atatcggtat gcttgtttgg ccgatgtttg cgattggtcg attgttcaac gtattggaaa
    35701 gaggaaatgc ttcttatgat cgcgtcaatg aacttttgca tgaaaaaacg catatcgtgg
    35761 aacaacttgg tgcagtgcag accccggcaa aaggcgagct gaatgtagct atttcctctt
    35821 ttaattatcc gaaagaagaa tcaccagcat tagaacaaat caagtttgca atccaagaag
    35881 gagaaacatt aggtattgtt gggaaaactg gtgcgggaaa aacgacgatc atcaaattac
    35941 tgatgcgtga atacgatgat tatcaaggga aaatccaatt tggcggacgt tcgatcaaag
    36001 aatattcatt gaatgcttta ttaggatcaa tcggctatgt gcctcaggat cacttcttgt
    36061 tttcgatgac cattcgagat aatatccgtt tttctaatcc aaaactttca gaagaagaag
    36121 tagagcatgc ggcttcttta gcatttatca acaatgaaat caaggaatta ccaaaaggtt
    36181 atgatacctt agttggagaa cgtggagtct ctttatctgg cggacagaaa caaaggattt
    36241 cgattgctcg agcattgatc aaaaatcctg aattattaat attagatgat gcactttctg
    36301 cagtagatgc caaaacagaa gaagctatct tgcataattt aaaagaaata agaaaagaga
    36361 aaacaactat catttcagct caccgtttaa gtagcgtgat gcatgcaaaa gaaatcattg
    36421 ttgtcgatca agggaaaatc attgaacgag gaacccatca tgagctgctt gcactagggg
    36481 gctggtatga acaaatgtgg gagaaacagc aattagaagc gaagatagaa ggaggcgagg
    36541 cctgatggat gaaaattatc aatcggaatg gacaaaatcg attccactta aagaacagaa
    36601 aaagattatc aaacgactga tgaagttcgc caaacctttt agaagaacgt tcatcgttgc
    36661 tatcttattt gcgtttgctc tgtctgtgat caatgtccta ttgccaagga tcatccaaac
    36721 ttttatggat gatcatctgg ctaaacagtc ggctacgaca caagttattt tgttttttgc
    36781 tggtatgtac ttatttggag tcatagtcaa aagtatcatt tggtttttcc aatggtattt
    36841 gtattccatg gcttcattga agacctatca gtatattagg gtaaaattat ttgaaaagct
    36901 gcatacacta ggtatgcgtt attttgatca gacaccggca ggatcaaccg tttctcgagt
    36961 gacgaatgac acagaaacgc tatttgaatt ttggtacgtc tttttgatgg tattgactgg
    37021 gatctttgca gtcgtttctt cattcattgc catgtttcaa atcaatggag aaattgcctt
    37081 gtggactttg gtgttcttgc caattttagg aatcgtcatc tggtattacc agaaatttag
    37141 ttctcgtatt taccggaata tgcgagaaaa attaagtcag ctgaatacga aattgaatga
    37201 atcgatttcc ggcatgcgta ttatccagca gtttcgccaa gaagctcgtt tagctaaaga
    37261 atttgaacaa gtgaacaatg agtatttaga cacgcgctat gcgatgatca aaacgaattc
    37321 gattctgtta agtccaatca tcaattttct atatgcattg gcgattgcgt tggtgttgac
    37381 tttgtttggg atcgatgcgt tgcattctcc cgtagaagtt ggactgatct atgcatttac
    37441 gacctatgtc cagggatttt ttaatccaat gtctcaaatg atggactttt tgagcatctt
    37501 cacagatggg atggttgctg gtagtcgtat cttgaagatt atggatacag aagaatatgc
    37561 accacaacaa aaccctggag cagatcaaat gattacttca ggtaagattg agtttcgcaa
    37621 tgtaagcttt tcttatgatg gagagcacaa tgtattaaac aatatctctt ttgttgctaa
    37681 tccgggagag accgttgcat tagtgggaca caccggaagt gggaaaagct cgatcatcaa
    37741 tgtgttgatg cggttttacg aattttatga aggtcagata ttgattgatg atcatgacat
    37801 tcgagaatat ccaatggaag agttacgtaa gaaaatgggg ctggtcttgc aagatgcttt
    37861 cttgttttat ggggatatcg caggaaatat tcggatgatg aatccagcta tcacagacga
    37921 acagatacgc catgcggctg agtttgttca agcaaacaaa ttcatcgatg aattaccaaa
    37981 aggatatcac tcaaaagtga tcgaacatgg tgccagctat tctagtggac aacgacagct
    38041 gatcactttt gcgcgaacaa tcgtgactga tcctaaaatt ctagtattgg atgaagcaac
    38101 agcaaatatc gatactgaaa cagaaggatt gatccaagaa ggattgacaa aaatgcgaca
    38161 aggacggaca acgattgcga ttgcccatcg gctgtccacg attcgggatg cagaattgat
    38221 tttagtttta gacaaaggtc atatagtaga atgcgggaat catgaaacac ttctagcgca
    38281 aaatggtttg tatgcagata tgtacaaact gcaaagcagt ggtgtgtaaa aagtggttgt
    38341 gacagggctc cgttcagctt taggtgataa gaaaaaaatt gtaaaaaagg ctttccagat
    38401 ttttgcataa atttggctta tcaccgataa gctggtactg gaacaccgta tattagaagt
    38461 tgggacagta gcatagtagc attttgacct aagcactaga gtgaaaataa aacagagctt
    38521 cccagattct tcatctgtga gcgttagtgt cgtaattacc agatttaaaa aaaatcacga
    38581 cagctagtca gaagtgccca atagtcgcta agaaagcgtt ggcaaaagtg cttgctttcg
    38641 ataatataaa gaaaacgata caacttggat agtgattgcc tgatgcaagc taaacctgat
    38701 gagtctccca tggttccatg gacggctttt tgggtttttc tttttgtttt ggatacagcc
    38761 ctatatatca tggagaggag taaaacatgc tgattgagaa agtactgaat aacaacgtcg
    38821 taataaccag ggatgaaaga aatcaggaaa tgatcgtgat gggaaaaggt ttggcattca
    38881 aaaagaaagt gggtgacccg atttttgact catgcatcga caaaaaattt cagttgacaa
    38941 atgacggact ttcacaacag ttccaagaat tggcacaagc gattcctctt gagtacttag
    39001 aaatttcttg tgaaatcatt gaatatacaa aagaggctct acaagtccag ttgaatgatt
    39061 cggtgtatat tgcattgacg gatcatttgt atacggctat cgaacgtcac aaaatgggaa
    39121 tatttgtacc aaatgtattg ctgtatgata tcaaacaact atttcctaaa gagtatgcca
    39181 tcgggaaaaa aacagtgaag aaaatctcgg ataaatacca catcaagtta tcagaagatg
    39241 aagccggatt tatcgcattg catttagtca atgcacaagg cattcaaagc caacaagaca
    39301 atacctacct gtttactaca acaatacagg atattctaaa tatcgtccgc tattactata
    39361 agattacatt tgatgaagat tcagtccatt tttttcgatt cgtcacacac ctacgttttt
    39421 ttctgacacg cgtcctagat ggagaaaaat cagaagtggg agaagtttct gaggaattgt
    39481 accaggttat tcaaaaaaaa tataaggata cgtttggttg cgttgagaaa atagcagact
    39541 ttttagccga gatattcaac taccagatat ccacggacga aaaaatgtat ttaacgatcc
    39601 atactgcgcg tttagtgcat gagaatcgtt gtaaataaat aaaaaaagga ggataaatta
    39661 acaagaacgc atgaaataag ggtagtgaca ttcagttggc taaaccttcc actaataaat
    39721 tggagggaat agaaatggga aaatatcaag aattagcgag caaaattgta gaaaatgtcg
    39781 gcgggaagga aaatatcaat ggtttgacac attgtatcac aaggcttcgt ttcaaattaa
    39841 aaaacgaaga aaaagcaaat gatgagatct tgaaaaatat ggacggtgtt gtgacggtaa
    39901 tgcgagcggg aggacagtac caagttgtca tcgggaatca tgtgccagtc gtgtttgaag
    39961 aggttataaa agcaggaaac cttacttttg atgaggctgt ttcaacagaa aaaatgagac
    40021 cgtttgatat gcttattgat ggaatatcgg gctgtttcca gccattcctt gctattctag
    40081 ctgctggtgg gatgattcgt ggactgacag cattacttgt cttcttaggc gcatttgacc
    40141 gcggttctgg cacatttgtt atgtttgata atatcggtga ttctgtattc caatttatgc
    40201 ctgttattat cggtttaaca gctgcccgta aatttaaagt aaatgaattc gtagggatgt
    40261 tgcttggcgc agcattaatg aatcctagct tgtcgctgga agctttgagt ggtgcagcag
    40321 aagcacctat ttatcaaaca gtattcggca ttccctggat tgcgcgtaac tacgcttctt
    40381 ctgtcattcc cattatcttt attgtgttgt tagctagcca aattcagaag ccaattaaaa
    40441 agttagtacc tgagatgatc gcgaatttct tcgttccatt cttcaccgtt cttattacaa
    40501 tgcccttagg ttttttactc gttggaccag cattcacatt cgcaactgat attttaatgg
    40561 ctggatttga aacactttta gcactttcgc cagttattta tggagcaatc gtaggcttct
    40621 tctggcaaat tctggtgatg tttggtctcc attgggcaat cgtaccaatg ggattgatgc
    40681 aattttctgt gaatggttgg caaaatatca tgacacctgt tgcagtagtg tcttttggat
    40741 aaactgcagc acttactgca ctctacttta agcttagaaa cccaaaagac aaagcaattg
    40801 caataccagc cattgtttct ggtattgtgg ggattacaga gccagctatt tatgggttta
    40861 cattgccacg taaaaaaata tttattttta cctgtgtcgg tggtgcgtta gggggcgcgt
    40921 atagtggatt aatgaattta acttcttgga atcaaggtgg actaggcatc tttacaattc
    40981 ccaactatat tagaccagat ggtgatttaa cggatgtgat taatgtattg attgggatcg
    41041 ccattgcgat ggtggtatcc tttgcgctga cttttttctt ttggaaggag gaagcaggtg
    41101 aaacagatga tagacagaaa agatcaggta aagaaattgt aaaaacaccg atacaaggac
    41161 aaatcgctcc actgaatgca gcaaaggatg cagcttttgc acaaggaaca ctaggtcgag
    41221 gaatcttgat ctaccctgaa aaaggagaag tacgtgcccc ttttgacgga acagtcatga
    41281 ccctgttccc ggcgaaacat gcaatcggaa tggtttctga agcaggtctg gaattgctga
    41341 ttcatgtcgg attggatact gttcagttgg aaggaaaata ttttgaaagc ttggtgcagc
    41401 aaggagaaag agtgaaacaa ggcgatctgt tgctccgttt cgacaagaag aaaatcgaag
    41461 aggaaggata cagtttagaa acacctgtga tcgtcaccaa ttacacggat tatttggata
    41521 ttttggaaaa tcaaggggag acggtcgggc cagacgatgc acttatcacc gcactgacct
    41581 gaacggttag gtgaaagaat ggactaggct taactattct ttttattcca cttctttatt
    41641 ttcgaaattg aaaggctctt tcatgatggc cgagctgata gggcgtttat cgaatttttg
    41701 tcctacagag attagatgga ctaaattctg ttgataattt tctgcaaaaa cacaactata
    41761 taacacatct agaagataac aaatcgatgt attgatcgta aaattaccaa ttttcgaata
    41821 cagtcgttcg cgtgtcgtaa gtcgcaaagc ggctgtactc attgaagata gcgttgaatc
    41881 accgatactg gtcaaagcta aaattggaac atgattttgt ttcaatattt ctgcggtacg
    41941 aaggatcaca ttgttttcac ccgtataaga aatcagaatc ccgcacatat cactcgtgca
    42001 attgtacgct tcatagaacg attcaccaaa agtggtgaca atcgtgacat ccattttgat
    42061 gcgcttcatt ttcagcatga aatcttgaga aatcaaagta ttcgagttac tggcgaaaat
    42121 cacgattttt tttgcttgaa gcattagttg tttggctttt tgcagttggt catgatgaat
    42181 gagagaaagt gtatcttgaa tcgtcatctg ttctaaagaa gccattttat ttgcgatcgt
    42241 cataataccg tctgtggtgg aaaaaggaag attcgcatct acgtcttgaa aattcccttc
    42301 aagataggct gtttcatcta aaaaagcatt acgaaaactg acccagccat caaaacccat
    42361 tttttttgct acacggatga gtgtggacgg gtggacataa catcgctcag caatctcttg
    42421 gatggtcatt tcgccaagtg ctggagaatc gcttaagaga aactgaatca cttcattttc
    42481 tgaaggagaa aaggagattt gtttgatttt ttcacgtaat aacatgtaga taccttcttc
    42541 ttgttttttt acgtaaatta taactttttt gaaaatgatt tgtaaacgga tttatgttat
    42601 atagcttaat aaagacattt tttgacaaga tatttacaaa aatccagaag aaaacactaa
    42661 cttttgtaaa tgttactgga aagaaacgaa tttctattgt ttgttttgtt tacaaaaaag
    42721 ataataaaag tgtaaaaagc aaaggagtga gaacatggca aaaggaatca aaatcgctac
    42781 gattggtgga ggcagcagtt atacccccga gttgatggaa ggatttatca aaagatatga
    42841 ggaattaccg atacgagaaa tctggctcgt agatgtagaa gaaggaaaag aaaagcttga
    42901 gatcgtcagt cagatgtctc agcgtatgtg ggatgcttct ccgtatgaag tcaaaatcta
    42961 tgcaactctg gatcgggaag aagcattgaa agatgcggat tttgttacga ctcagtttcg
    43021 tgtcggtcaa ttagatgcac gaatcaaaga cgagcggatc cctagttatt atgggatgat
    43081 tgggcaagaa acaaatggtg ctggcgggat attcaaagct tttcgtaccg ttccagtggt
    43141 tttggcaatc gttgaagaca tgaagcgttt atgtccaaat gcttggctga tcaatttcgc
    43201 gaacccaagc gggatggtga cagaagcagt tatcagatat ggcaaatggg ataaagtgat
    43261 cggattatgc aatgtgccag taatggcgat gatgacagaa ccaacaatgc ttggcaagaa
    43321 aaaagaagag ctgatctaca agtttgccgg tctaaatcac ttccattggc acaaagtagc
    43381 agataataaa ggaaatgacc gcacggcaga attgattgat cgtttatacg atgaagacaa
    43441 tggattgccg aaaaatatct ttgacatccc gtttttcaaa gaacagttaa aacaaatgaa
    43501 catgatccct tgtggctacc accgctatta ctaccgtgaa gaagaaatgc tggaacatgc
    43561 tttagaagaa tatcaaacga ttggtacaag agcgcagcaa gtgaaagaaa cagaagcaga
    43621 gctgtttgaa ttatacaaag atccagaatt ggatcataaa cctgaacaat tgcaaaaacg
    43681 aggtggtgct tattattcag atgctgcctg tgaaacgatt gcttcgatct atggaaataa
    43741 agaaacacag atcgtggtat ctacaaagaa tgatggagct gttcctgatc tgccggcaga
    43801 ttgtgtagta gaagtcactg cctatgttgg cgcacaaggc gcacgaaatg tagcgtttgg
    43861 ttcccttcct gctgctgaaa gaggctggtt gcaagtaatg aagaatatgg aattgctgac
    43921 gatcgaagca gccgtgactg gtgattatgg aacagcttta caagcgttta cgatcaatcc
    43981 gcttatacga tcaggaaaga cagcaaaacg gatcatggat gagttgttta ttgcacatca
    44041 gcagtattta ccgcaattca aggaaacaat cagccggtta aaagcagaag gaatcgaagt
    44101 ccaagatgaa ctggcaagag aattatcaag caatacacat gtaatggagg aagtatgatg
    44161 ggattcagaa aagatttttt atggggcgga gctacagcag ccaatcaatg cgagggcggt
    44221 tatgatgaag gcggacgtgg gttagcgaac gtagatgttg tgccaatcgg agaagaccgt
    44281 tttcctgtaa ttactggaaa aaagaaaatg tttgaatttg atgaagatca tttttatcca
    44341 gcaaaaattg cgatcgatat gtatcatcat tataaggaag atattgcttt gtttgcagaa
    44401 atggggttca agacttatcg gatgtccatt gcttggagtc gtatttttcc aatgggcgat
    44461 gaaaaagagc caaatgaatc tggtttgaaa ttttatgagg atgtctttaa agaatgtcaa
    44521 aagtacggga tcgagccgct tgtgacgatt acacattttg attgtccgat gcatttgatc
    44581 aaagaatatg gtgcgtggcg ttccagagaa ctcgttggtt tttatgaaaa tctgtgccga
    44641 gtcatcttca atcgctataa agggcttgtc aaatattggt tgacattcaa tgagatcaat
    44701 atgatccttc atgcaccatt catgggagca gggctgtatt ttgaagaagg agaaaatgaa
    44761 gaacaaatca aatatcaagc ggcacatcat gagttggttg caagtgccat cgcaacgaag
    44821 attgctcatg aagtagatcc ggagaacaaa gtaggctgta tgctggcagc aggagcatat
    44881 tatccataca cttgcaatcc tgaagatgtt tgggcttcta gaaaatcaga tcgagaaaat
    44941 tatttcttca ttgatgtcca gtcacgagga gaatatcccg cttatgcatt gaaagaaatg
    45001 gaacgaaatg gcattcagat cgaaatggaa cctggggatg ccgcattgct gaaagaacat
    45061 actgttgatt ttatctcctt ttcttactat tcttctcgag tttcgactac tgacaacgaa
    45121 ctcttggaac agactgcagg aaatatcttt gcttctgtca aaaatccata tctggaagca
    45181 agtgaatggg gttggcaaat tgatccatta ggcttgagaa taacgatgaa tgatatttat
    45241 gatcgttatc aaaaaccact atttattgta gaaaacggct taggtgcagt cgatacgccc
    45301 gatgagaatg gttatgtgga agatgactat cgaatcgact atctggcagc acatatccag
    45361 gcaatgaaag atgcagtaga attagatgga gtcgatctat taggctatac gacttgggga
    45421 tgtattgatt tagtatcagc tggtacaggt gagatgaaga aaaggtacgg attcatttat
    45481 gtcgatcgtg acaacgaagg aaatggcaca ttagcccgtt cgaagaagaa atcattcaac
    45541 tggtataaaa aagtaattga ttcaaatgga gaaaatcttt cgaatgatta atcaatgatt
    45601 tataaaagtc tattgttttt cagcggattt ttcgagtata ataaagctat tcgagaaacg
    45661 caagcagaac aatagacttt ttttcgcatt ggctgtaaag tatgcttact tgattaagta
    45721 aaggagaatc tacatatgta tgatgtaatc gttattggcg caggtcctgc tgggatgact
    45781 gctgctttat atgcttcacg ttcaaactta tccgtattga tgatcgaaca aggcgcccct
    45841 ggcggccaaa tgaataatac ggcagaaatc gaaaactatc ctggtttcga ttcaatcatg
    45901 ggtcctgatc tagcttataa gatgtatgaa ggtgtttcaa gatttgggac agaaaatgct
    45961 tatggaatcg tgcaagatat caaggatcac ggcagccaca aagaagttat ttgtgaagat
    46021 aaaacttacg aaggaaaaac agtcattatc gcaacaggct gtatccaccg taaattaggc
    46081 gtacctggcg aggaagaatt tgctggacgt ggcgtttctt actgtgcagt ttgtgatggt
    46141 gcgtttttcc gtaacaaacg tctagtagtg atcggaggag gagactcagc agtagaagaa
    46201 gccatctatt tgacacaatt tgcttctgaa gtagtcatcg tccaccgtag agatgaatta
    46261 cgtgcacaaa agatcatcca agaccgtgca tttgcgaatg aaaaaatttc ctttatgtgg
    46321 gatacagtcg ttgacgaaat cgttggaaac gaaatggtag tgacaggtgt gaaagcacgt
    46381 aatgtcaaaa ccggcaatga atttgattta gcagcagacg gagtattcat ctatgttgga
    46441 ctggatccat taactgcacc ttttagagaa gcaggtttga cgaacgaagc tggctggatt
    46501 ccaacagaca acgaaatgaa aacgaatatg ccaggcgttt ttgccatcgg agatgttcgt
    46561 gaaaaagatt tgcgacaaat cactacagct gttggtgacg gcggtattgc tggacaacaa
    46621 gtgtataaat acttggagtc tttagaagaa acaacagcat aagtttaaaa agagtgaaag
    46681 aacggtttca attgttcttt tactcttttt tttcgcgtga aatccactga atccgattta
    46741 tgtgaatact tgcaatcatg cttgataaaa gaatcctttc ttgatttaag cgttttttgg
    46801 tcctcatact ttcatatcta ataagtgaat gctatactta ttaagaacca tatttcagaa
    46861 agaggtgtta ttttcatgtc ttgggaacaa gtgtatgaac aatgggtaca tgaagaaaat
    46921 ttagaagaaa acttaaaaaa acaattaaat gatctgggtg aagatcctga aaaacttgaa
    46981 gatgcttttt atgcaccgtt ggaattcggt actgcaggca tgcggggaat cttaggacct
    47041 gggatcaatc gaatgaacat ctatacggta agacaagcaa cagaaggact agcacgtttc
    47101 atggatacac aaggtgaaga aacaaaacgc cgaggtgtcg ctatcgctta tgactcacgc
    47161 catcagtcac cagaatttgc aatggaagca gccaaaacac tggcacatca caatatcccg
    47221 tcttatgtat tcgaaagtct gcgtccaaca ccagagcttt cttttgcagt tcgtcatttg
    47281 aagacgtttg cgggaatcat ggtaaccgca tctcacaacc cagcggctta caatggatat
    47341 aaagtctacg gagaagatgg cggacaaatg cctcctgcag acgcagatgc attgacgaaa
    47401 tatgtccgtg aagtcagcaa tccgctaaaa gtcgaagtat tatctgacga agaagctaaa
    47461 catagcgggt tgatcacgat catcggagaa gaagtggacg ctgcttatct agaagaaatc
    47521 aaagcagtga cgattgatcg tgaattggtt gaaacaatgg ggaaagattt gaaacttgtg
    47581 tacacgccac ttcatggaac agggaaaatg ttgggcgaac gtgcgttgaa acaagctgga
    47641 ttcgaacaat tcgtactagt tcccgaacaa gctgtggcag atccagattt caccacggtc
    47701 aaatcgccaa atccagaaga acattcagct tttgaatatg ctatacgact tggagaaaaa
    47761 gaaggagctg acttgctgat tgcaacagat cctgatgcag accgtttagg cgcagcggtt
    47821 cgtttgccag atggttctta tgaagtattg actgggaacc aaatcggagc attgtttgct
    47881 caatatattt tggaagcaca tcaatcaaga ggcactttac cagaaaacgc agcggtattg
    47941 aaatcaatcg tttccagcga attgcctaca gcaattgcgg aacactacgg tgctaccatg
    48001 ttcaatgtgt tgacagggtt taaattcatt gctgaaaaaa tccaacaatt cgaagaaaac
    48061 cattcacata cttttatgtt tggattcgaa gaaagttatg gttatttagt taaacctttt
    48121 gttcgcgata aagacgcgat ccaagcattg cttttattag cagaagtagc agcttactat
    48181 aaaaaacaag gcaaaacgtt gtatgacggc ttgcaaacga tttttgaaac ctatggctat
    48241 ttcgcagaaa aaacgatttc tgtgacaatg agcggacaag aaggttctgc gaaaatctct
    48301 gctttgatgg aaaaattccg taaagaagca ccgacagaat ttgccggagc aaaagttatt
    48361 caaacagaag actttaaaga attgacgaga acagaccgtg aaggaaacgt agaaaaactc
    48421 acaacaccgc catccgacgt attgaaatat acattggaag aaaatggctg gattgctatc
    48481 cgcccttcag gaactgagcc gaaaatcaag ttttatattg gtgtaaaagg aagttctaca
    48541 gaagacgcag ccgaaaaagt agcctcttat gaagcgatta tcaaaaagat gacagaagaa
    48601 taaaaaactt gagactgatg gaaaaagtgg taaactttag aagtcagtct tttttcaagt
    48661 tggaggtgaa tggattgcca gacacgcaaa aatcaaaagt agagaaagtc agtcaattat
    48721 tcaaaatgtt gagtgatccc actcgtctta aaattctgct atatctgaaa gacggggaac
    48781 aaaatgtgac agcaatcacc caagcagtgg aaatggagca gtctgctgta tcgcatcaac
    48841 tacgtttatt aagagaaaat catgtcgtca agtctcatcg cgagggaaaa gcgattttgt
    48901 atagtcttga tgattctcat gtactggata ttttgaacca gacactcaaa catgtggaac
    48961 atcaataacg ataggtgcaa atagataaac actggagtgt gacaacgaag aaatttcgtt
    49021 gtcacactcc agtgtttttt aagaaaaaga caaaaatgat caatcgcttt tttctgttgt
    49081 ttcagttcca taataactat gatcttctag gtctaaagca ttcaaaataa gcgaagccgt
    49141 ttcttcaata gataaagatg ctacattgat cactacacat cctagttttt cataaagatc
    49201 attcgcaaaa tcgagttcag ctctaatttt ttcgatatcg gaataagcag tatcagggtt
    49261 taatccgtaa gagcgcatcc gttctttacg gatattattt aaaatatctg ggttatttgt
    49321 tagtcccacg atttttttag gatcgatctc ccaaagctgt ttgggaatat gtgcttgtgg
    49381 gatcaaaggt aaattggcaa cttttaaatt tttgttagct aaaaacaagc tcaaaggcgt
    49441 tttggaagtt cgggatacgc ctagcaaaac gacatctgct tctaaaaaac cacgaggatc
    49501 tttcccatca tcgtatttta cagcaaattc cattgctttg atccgtttga aatagttctc
    49561 attcaataaa tggagggcac caggttgccg aattggcttg ataccagtta aacgttcaat
    49621 ttctgatatc ggtggcgtca tcacatcaaa actaaataat tgatgttctt cacaaaaaga
    49681 acgtgttatc tcgatcagtt cttgattgat taatgtatgc aggacaagtg ctttttcttc
    49741 taatgcatct tgcaatgcct gccttaatgt tttttcatct gtcacgaacg ttctgcggaa
    49801 taggttgaac tcaacggtag gatactgagc catagtagct tgcgctagtt ttgtcgcagt
    49861 ttctccagca gagtcggaga tgacaaaaaa agtaacgatt tctgtagaat cggaagtcat
    49921 ggataaaacc tcacttgcat tttttttatg tttattatac catactctag aatgcgtact
    49981 tcgtaatcat tttgatttgg agggataagt catgaaaaaa cagacattga tcgaacaatc
    50041 attagaaaca ttgaaaaata acggttttaa atatactaaa aaaagagaag cattgttaac
    50101 ttatttagtg aagcgtaacc gctacgtatc tgctaaagaa gtttttgact atatgaatca
    50161 ggaattcaaa ggggtaagtt atgatacgat ttaccggaat ctccatgatt ttgcagagat
    50221 cgatttactg gaagaaaccg atttaaatgg agaaatgaag tttcgttttc attgctgtca
    50281 ggggaaaatc ggccatcacc atcattttat ctgtacagtg tgcggaacaa cgaaagaaat
    50341 ccatatgtgt ccgatgaatt atttcgaaga acagctaggt ggatgcacga tcgaagggca
    50401 tagattcgag attttcggaa gatgtgaaaa ttgtcaataa ggatttaaat aaaataacat
    50461 ttgataattt taaaaaaaag caagatttcc ttgacggtca aaacagcatt agatataata
    50521 tttacaggac agtcttgttt tttgagatat atttcaataa caattacggt tactttttaa
    50581 gctcggaggg agggaattaa catgtcaaaa acagtcgttc gcaaaaacga atcacttgac
    50641 gatgctcttc gtcgcttcaa acgttccgtg tcaaaagcgg gtactttaca agaatctcgt
    50701 aaacgtgaat tctacgaaaa accaagtgta agacgcaaga aaaaatctga agcagctaga
    50761 aaacgcaaaa aattctagtt ctgtgatgaa ttggagttgg tagtgtgtca ctactaagta
    50821 gattaaacga tgacatgaaa gcagcgatga aagcgaaaga taaggaatct ttacaagtca
    50881 tccgaatgat caagtcatct atacaaaacg agcagattaa agaaggtcac gacttgacag
    50941 aagaagaaga actaacggtg ttgtctcgtg aaatgaaaca gcgacgtgat tctttgcatg
    51001 agtttgaaga agcaggacgt gacgatttag ctgaaaaagt aaaaagtgaa attgtaatag
    51061 tcgaaaaata tatgccggaa caactttccg acgaagaaat tcgacaactt gttcaagaag
    51121 caatcactca aactggtgct tcatcaatga aagaatttgg caaagttatg ggtgcaatta
    51181 tgcctaaagt caaaggaaaa gcagatggaa accaagtgaa tgcgattgtg aaagaactat
    51241 tacaatctta attttatcat ttaaactgcg gacagtgatt atccgcagtt ttttgtgttc
    51301 gta
//
LOCUS       DACDUU010000011        50025 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.011, whole genome shotgun sequence.
ACCESSION   DACDUU010000011 DACDUU010000000
VERSION     DACDUU010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.011"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..169)
                     /locus_tag="IWL30_000667"
     CDS             complement(<1..169)
                     /locus_tag="IWL30_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP9705456.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVN"
     gene            complement(171..368)
                     /locus_tag="IWL30_000668"
     CDS             complement(171..368)
                     /locus_tag="IWL30_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAP9705457.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(762..2489)
                     /locus_tag="IWL30_000669"
     CDS             complement(762..2489)
                     /locus_tag="IWL30_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9705458.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2482..3675)
                     /locus_tag="IWL30_000670"
     CDS             complement(2482..3675)
                     /locus_tag="IWL30_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9705459.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3862..4506)
                     /locus_tag="IWL30_000671"
     CDS             complement(3862..4506)
                     /locus_tag="IWL30_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9705460.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4600..4734)
                     /locus_tag="IWL30_000672"
     CDS             complement(4600..4734)
                     /locus_tag="IWL30_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAP9705461.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4736..6868)
                     /gene="feoB"
                     /locus_tag="IWL30_000673"
     CDS             complement(4736..6868)
                     /gene="feoB"
                     /locus_tag="IWL30_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAP9705462.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6865..7338)
                     /locus_tag="IWL30_000674"
     CDS             complement(6865..7338)
                     /locus_tag="IWL30_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAP9705463.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7739..7963
                     /gene="nrdH"
                     /locus_tag="IWL30_000675"
     CDS             7739..7963
                     /gene="nrdH"
                     /locus_tag="IWL30_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAP9705464.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8122..10281
                     /gene="nrdE"
                     /locus_tag="IWL30_000676"
     CDS             8122..10281
                     /gene="nrdE"
                     /locus_tag="IWL30_000676"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAP9705465.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10331..11296
                     /gene="nrdF"
                     /locus_tag="IWL30_000677"
     CDS             10331..11296
                     /gene="nrdF"
                     /locus_tag="IWL30_000677"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAP9705466.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11385..12524)
                     /locus_tag="IWL30_000678"
     CDS             complement(11385..12524)
                     /locus_tag="IWL30_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9705467.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12833..13546)
                     /locus_tag="IWL30_000679"
     CDS             complement(12833..13546)
                     /locus_tag="IWL30_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAP9705468.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13800..14501)
                     /gene="nagB"
                     /locus_tag="IWL30_000680"
     CDS             complement(13800..14501)
                     /gene="nagB"
                     /locus_tag="IWL30_000680"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP9705469.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14735..16267
                     /locus_tag="IWL30_000681"
     CDS             14735..16267
                     /locus_tag="IWL30_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9705470.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16458..17660)
                     /locus_tag="IWL30_000682"
     CDS             complement(16458..17660)
                     /locus_tag="IWL30_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9705471.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17742..18431)
                     /locus_tag="IWL30_000683"
     CDS             complement(17742..18431)
                     /locus_tag="IWL30_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP9705472.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18537..18905)
                     /gene="rplL"
                     /locus_tag="IWL30_000684"
     CDS             complement(18537..18905)
                     /gene="rplL"
                     /locus_tag="IWL30_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAP9705473.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(18958..19461)
                     /locus_tag="IWL30_000685"
     CDS             complement(18958..19461)
                     /locus_tag="IWL30_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAP9705474.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(19871..20560)
                     /gene="rplA"
                     /locus_tag="IWL30_000686"
     CDS             complement(19871..20560)
                     /gene="rplA"
                     /locus_tag="IWL30_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAP9705475.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20664..21086)
                     /gene="rplK"
                     /locus_tag="IWL30_000687"
     CDS             complement(20664..21086)
                     /gene="rplK"
                     /locus_tag="IWL30_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAP9705476.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            21429..22097
                     /gene="sdaAB"
                     /locus_tag="IWL30_000688"
     CDS             21429..22097
                     /gene="sdaAB"
                     /locus_tag="IWL30_000688"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAP9705477.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            22110..22982
                     /gene="sdaAA"
                     /locus_tag="IWL30_000689"
     CDS             22110..22982
                     /gene="sdaAA"
                     /locus_tag="IWL30_000689"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAP9705478.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(23073..24092)
                     /locus_tag="IWL30_000690"
     CDS             complement(23073..24092)
                     /locus_tag="IWL30_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP9705479.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(24265..25152)
                     /locus_tag="IWL30_000691"
     CDS             complement(24265..25152)
                     /locus_tag="IWL30_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9705480.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(25229..25774)
                     /gene="nusG"
                     /locus_tag="IWL30_000692"
     CDS             complement(25229..25774)
                     /gene="nusG"
                     /locus_tag="IWL30_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAP9705481.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25875..26045)
                     /gene="secE"
                     /locus_tag="IWL30_000693"
     CDS             complement(25875..26045)
                     /gene="secE"
                     /locus_tag="IWL30_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAP9705482.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(26069..26221)
                     /gene="rpmG"
                     /locus_tag="IWL30_000694"
     CDS             complement(26069..26221)
                     /gene="rpmG"
                     /locus_tag="IWL30_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP9705483.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            26383..27027
                     /locus_tag="IWL30_000695"
     CDS             26383..27027
                     /locus_tag="IWL30_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9705484.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(27083..27586)
                     /locus_tag="IWL30_000696"
     CDS             complement(27083..27586)
                     /locus_tag="IWL30_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP9705485.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27599..27645)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            28183..29148
                     /locus_tag="IWL30_000697"
     CDS             28183..29148
                     /locus_tag="IWL30_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP9705486.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(29247..30419)
                     /locus_tag="IWL30_000698"
     CDS             complement(29247..30419)
                     /locus_tag="IWL30_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9705487.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(30406..31551)
                     /locus_tag="IWL30_000699"
     CDS             complement(30406..31551)
                     /locus_tag="IWL30_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9705488.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(31565..32489)
                     /locus_tag="IWL30_000700"
                     /pseudo
     CDS             complement(31565..32489)
                     /locus_tag="IWL30_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            32606..33685
                     /locus_tag="IWL30_000701"
     CDS             32606..33685
                     /locus_tag="IWL30_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9705489.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            33717..34310
                     /locus_tag="IWL30_000702"
     CDS             33717..34310
                     /locus_tag="IWL30_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9705490.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(34420..34647)
                     /locus_tag="IWL30_000703"
     CDS             complement(34420..34647)
                     /locus_tag="IWL30_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9705491.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(34750..37338)
                     /locus_tag="IWL30_000704"
     CDS             complement(34750..37338)
                     /locus_tag="IWL30_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAP9705492.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(37358..38017)
                     /locus_tag="IWL30_000705"
     CDS             complement(37358..38017)
                     /locus_tag="IWL30_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9705493.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(38066..38569)
                     /gene="trmL"
                     /locus_tag="IWL30_000706"
     CDS             complement(38066..38569)
                     /gene="trmL"
                     /locus_tag="IWL30_000706"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAP9705494.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(38790..39656)
                     /locus_tag="IWL30_000707"
     CDS             complement(38790..39656)
                     /locus_tag="IWL30_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9705495.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            39765..40406
                     /locus_tag="IWL30_000708"
     CDS             39765..40406
                     /locus_tag="IWL30_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAP9705496.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(40468..41829)
                     /gene="mgtE"
                     /locus_tag="IWL30_000709"
     CDS             complement(40468..41829)
                     /gene="mgtE"
                     /locus_tag="IWL30_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAP9705497.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(41847..42746)
                     /locus_tag="IWL30_000710"
     CDS             complement(41847..42746)
                     /locus_tag="IWL30_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP9705498.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(42748..43545)
                     /locus_tag="IWL30_000711"
     CDS             complement(42748..43545)
                     /locus_tag="IWL30_000711"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAP9705499.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(43523..44227)
                     /locus_tag="IWL30_000712"
     CDS             complement(43523..44227)
                     /locus_tag="IWL30_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAP9705500.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            44358..44933
                     /locus_tag="IWL30_000713"
     CDS             44358..44933
                     /locus_tag="IWL30_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAP9705501.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            45051..45728
                     /locus_tag="IWL30_000714"
     CDS             45051..45728
                     /locus_tag="IWL30_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP9705502.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(45807..47615)
                     /gene="pepF"
                     /locus_tag="IWL30_000715"
     CDS             complement(45807..47615)
                     /gene="pepF"
                     /locus_tag="IWL30_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAP9705503.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSDAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(47791..48789)
                     /locus_tag="IWL30_000716"
     CDS             complement(47791..48789)
                     /locus_tag="IWL30_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAP9705504.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(49015..49668)
                     /locus_tag="IWL30_000717"
     CDS             complement(49015..49668)
                     /locus_tag="IWL30_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAP9705505.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT        14969 a        10495 c         8777 g        15784 t
ORIGIN      
        1 aattcaccaa tctatttatt aaaggaccgg gatctaattc atatttttgt ttttctaatt
       61 ttaaatatac cttttgtaaa acctcaagaa tatcagaata tttaggatct ttacttatag
      121 atatatcatt atataattca tgaacaattt gcttagcatt ttttttcatt ttagcacttc
      181 cctggaattg ctccacctaa aaaaccacct atagacattc ctgcaataca agtagttgcc
      241 ttggcccaat cgaccgtaca tttattttta gtgcaataca ctccatttcc ataatatttt
      301 ccactatgag tggtaccccc atagataagt tgtgtctctt taatagacaa aatttttaaa
      361 tgtttcataa tctaatcacc cctaaattaa tgtttttaga ctattattct aacattcatt
      421 aaatttcaaa aaacaattcc ttcttgaaac gtagcaaaat aattcctgaa taagaattta
      481 atcactttta agtatataaa ctatgtatat tctttaatat tttttgagaa tgcacttcgt
      541 acataataaa ctttaatcac attagtcttt catacctacg atcgatctct ttgactcgca
      601 cccataaatt cataatagtt tgtcggcgtc tttggacgtt tcgaagatga gctacgcatt
      661 ctcttttttt atagacaaaa aagatagaga cgaacatgga gcttccaaat ttggatattt
      721 gatccaattt tggaagctcc atttatttcg tgtctgtctt tttatttttt ccagctttcg
      781 actaattctt ggttctcttt gatccagtct tttgctgctt gctgtggatc ttttccattg
      841 ttgatttcca acataacttt ttccatatct ttttctgacc aatggaaatt gtctaatact
      901 ttgtaagctt ctggctggtc ttctttcaat ccttttcgaa ccatcgtatg gatctgctct
      961 tcgcttccca ttccgttttc aggatcttct aagtatttca gatcatattt ggcaaacatc
     1021 caatgaggtg tccatcctgt aactacaatt ggttcgtgtt ttttgatcgc ttgacctagt
     1081 gcaacggtca ttgctcctga agaagatgtc tctactttcc agtctttcaa gttgtcatat
     1141 ttttggatcg tgttttcagc agccgtaact acaccagctc ctggctcgat tcctatgatt
     1201 ttcttacctg cctgatcggt taactcatcg atcgaattta catccatgta ggctggcact
     1261 gctaaaccaa cttttgctcc tgtcaaattg gcacctaggt cttcgacttg atctttatat
     1321 tgtgcatatt gtgaaccatg agttttaggt agccaagctg aaaccatggc atctgactcg
     1381 ccatttgaaa cagatttcca catgattgaa ttatctaatg gcgtcatcgt tacatcatag
     1441 cccatttgtt tcaatacttc accgactaca tttgttgaag caacttctgt atcccattca
     1501 acataagata agttgatacg ttttgtttct ttggcagatg aaaaactccc tataccaatc
     1561 gcacctaaga tcactactac tacagcacca atgatcagtc cttgacgttt tttacttttc
     1621 tgtgttgttg ctcctgcagc ttttttattg tttggctggt tcaaatgctg tgtaaaacgg
     1681 tcaatgatga ttgctaagat aactaatgca accccgttga caaatccatt cccgacttgt
     1741 gctctttgca atgctgataa tacgccgcgt cctaagccgg gtgcaccgat catagaggca
     1801 ataacgacca ttgaaagtga cagcattgtt gtttgattga tccctgccat gatcgtactt
     1861 ttagctaaag gcaattctag tttgaatagt ttttgacgac ttgtgctccc gaatgaatca
     1921 gaagcttcta ctaattcttt aggaacttgt cgaatcccta aattcgtaaa acgaacggtt
     1981 ggtggcagag caaagatgac tgaggcaaat acccctggaa ccatcccgat accgaaaaat
     2041 gcaactgctg gaatcaaata aacgaatcct ggcattgtct gcataaagtc aaggatcggt
     2101 ttgataatac tttgggcttt actgcttttt gccatcaaga tccctaatgg taccccaatg
     2161 atgatcgaga tcacactaga taaaagaaca agtgtgacag tattcatcaa atcattccat
     2221 agaccttggt tataaataaa ccatagaccg attaatgtga acaacgtaag tcctggacgt
     2281 ttcttagaaa taaagaaagc tgctaccgtc aataacaaga taaacaaagg agctggaatc
     2341 actaacagta aatttgtaat ccccgacatc actgcctgtc caatcgtttg taaaaatgaa
     2401 aataggccag aaaaagttgt cgtaaaccat tctgtgatat tttctaccca gtttgccact
     2461 ggtaattgat agttcaatag attattcatg ttcactcacc tcgcttgttt ctgctaatgc
     2521 ttcaatcaca ctacctcgga tgacgacacc ttttaatttt ccatcctcta caactgcaag
     2581 tggagtcggt gagtcataaa taatattgaa aatatcactt accaccatgt ctttcccaat
     2641 caccgtcaca ttttcatcta ggaagtcgat caatggacgt ttttgcttcc tagcttctaa
     2701 cgcttgatct gcagtgatga tccctttcag gtgtctcttt ctgtcaacag ccatcagcat
     2761 gctgacttct tcattacgca tccttgtcaa tgcaacgttt ggtccatcag attcgatatt
     2821 cgtcgtcaat gcaggtacca tgatgttttg agccgtaagc actttggaac gatcgacttc
     2881 ttccacaaat tcacgtacat aatcatttgc tggattggtc aatatttctt cacctgttcc
     2941 gatctgcatg atttcgccgt ctttcatcaa ggcgattctg tccccaatcc gtaaagcttc
     3001 attcaaatca tgggtaatga aaataatcgt tttttgaaca ttggcttgca ggtccagtaa
     3061 ttcgtcttgc atttctcgcc ggatcagcgg atcaagagcc gagaacgctt cgtccatcag
     3121 caagatctca ggatcatttg ctaaagctct ggctaggcca acccgttgtt gcatcccacc
     3181 agataactga ctaggatatt ggtctttaaa tgaaagcaaa ctagaatttt ctaatgcttt
     3241 ttctgctttt tcttgtcgtt cttctttagg tacaccacgg acttctaacc cgtattctgt
     3301 attttccaag atcgttcgat gtggaaacag accgaagttt tggaaaacca tattgatttt
     3361 atgacgacgg acttctcgca attcttcttt gcttaacttc gatacatctt ctccatcaat
     3421 atagatactg cccgaagttg gctcaatcag acgattcaat agacggatca atgtggattt
     3481 cccactcccg gaaagcccca tgatcacgaa gatttctcct tcttttacgt caaaattcac
     3541 gtcatacaca ccaaccgttg caccggtttt ctttaaaatc tccgttttcg gctggtgatc
     3601 attcatcata tctaaagcgg cttgtgtctt tttaccaaat atctttgtta aatgttttac
     3661 ttctactttt accaaacaaa ttctcctttt ttatgtaaat tcatttttct ttctgaaaaa
     3721 agtgaccaca ctatgttaac atcatgtagt cactttgtca aaaatgaaag aaatttgctt
     3781 gtccaaactg aaaaaaactt ggctaaatct ttgtaaataa aaggtttagc caagttaata
     3841 tttttttaat atttcacaca atcattctgc aagctgatgc tccgggtaaa aaaagtttgt
     3901 catacgctgt agtgtatatt catttccaat aaaatgaacg atgtcccctg ccgtaaattt
     3961 agcatagggg ccaggtgaaa caagcagttc ttcattatga gaaattgcga taactgtggc
     4021 gccagtcgcc tgccagatat tcaactcgct aattgaacgg tccagatgca ttgctttttc
     4081 tgataaagat aattcaaacg gaagtaaagg attcatctta tgtattcgct tcgtttgatt
     4141 gaccagctga tccaataatt gagaaaaatg attcagctca tcttgttgct tctccacact
     4201 tgccatgatt tctgcttttg tatcttggat ggattgtaca tcttgatatt gcaataggaa
     4261 atcttttgct ttttccttcg aagcaacaaa tgccccactt ccatgccgta cttccatgat
     4321 tccaagatct accagcacat tgatagcctt gcgggtagtc tcaggagaag cattgtacgt
     4381 actggcaagt gttgagcggg catggatctt ctcacccact ttatatcggt tttcaacgat
     4441 acgttcggct aaatcaacag caatctgctg gtagcgcggg cggacgattt ttttctttcc
     4501 agacattttc tttcctcttt ctttttttac tttccctcat cttactatgc tctagcaaaa
     4561 taagcaaaaa aggggagaat agcctcttgc aacgactgat catcgcttga tctgttcctt
     4621 ttttactggg caagctgttt gacagtcatc acaattctta ccggatttta aacgagtata
     4681 gacaattgcg cctgcacttc caaagattaa cacacttaat aagaatgtcg ccatcctatt
     4741 cctctccttc taatgacaat atagtataaa actggttctt ggattttggc tgacggaaca
     4801 aaacataaac gagtcctatc atcagtaaaa tagcaagata cgtccctaac gctacctgtc
     4861 caccctccaa taagtgtccc aactgataaa tcacaaaact aactaaataa gctaaaccgc
     4921 attgatagcc tatggcgatc caagtccatt tcttggcatt catttcccga tggatagccc
     4981 caattgccgc aaaacaaggc gcacaaagta aattgaaggc taacaatgaa taagctgctg
     5041 cgggagtata tgtgtgttgt agcacttgcc agatttctct gccattttcg gatacttccg
     5101 catgaccaaa caaaatacca aatgtgccaa taatattttc tttagcgacc aatcctgtaa
     5161 tcgctgcgac tgttccttgc cagtttcccc aaccaagcgg ctgaaaaaca actgccagga
     5221 ttcgcccgaa aaaggctaag atactttggt cttcagaaac aggttgagca taaaaattat
     5281 aagaagagct gaaccagatc acgatactag aaacaaagat aatcgttcct gcttttttaa
     5341 caaaagactt gctgcggtca taggtttgtc ttaggaccgc tgaccattga ggaaaatgat
     5401 atgcgggcaa ttccatgata aaaggagctg tatctccagc aaacaaacgt gtttttttca
     5461 agctgattcc agagaaaata attgcagtca taccaagaaa ataggcagaa ggagctaccc
     5521 aagattgatg aggaaaaaag gcccctgcta ctaaggcaat aatcggcaac tttgcagaac
     5581 aaggcatgaa ggttgtgacc atgatcgtca aacgacgatc ttgctcgttt tcaatcgtgc
     5641 gactggccat gacgcctggc actccacatc ctgtagcaat cagcatagga atgaaggatt
     5701 ttcctgaaag accgaatttc ctgaaaagtc gatccatgac aaaagcgatc ctcgccatat
     5761 atccgcagtc ttccagaaag ctcaagcata aaaacaatat aatcaattgc ggtagaaatc
     5821 ctaacactgc tcccacgcct gcgatgatgc cgttcaagat cagctcctgc atccatcccg
     5881 ctacttgcca ttcagctaac aatctgccaa catgttctgg taaccatgta ccaaacaatg
     5941 tgtcattgat ccaatctgtc cccattgttc ctactgtttg aatagccaga taatagatca
     6001 gccacatcac aaatgcgaag atcggcaagg ctaaccaacg attcgttgca atctgatcga
     6061 ttttgtcact catcgataat tggaaagaat catttgacac aacacagagt gtacatagtt
     6121 gggtaataaa ttcataacgt tcattgacca gaatcgtctc actatcgtcg tccaatagtt
     6181 tctctgttaa ctggatcagt tgctcgatct ctttttgctg agaaactgct atctcgtaat
     6241 cctctcttac cttctgatct cgttcaaaat atttcattgc ataccagcgt ttcttgccgc
     6301 tttgttctcc tagtacatct tcaatttcat gtaaagctgc ttctaaacgt ggatcatatg
     6361 tacagggaac aggaatagaa ggccgctttt ttgcagactc ctgaatcttt tcgattagtt
     6421 tttcgattcc ttgctttttc aatacgctga ttggtaaaac tggtacacca agaccatagg
     6481 aaagcttttc tagattgatt ttttttcctt cttttttgat gacatccatc atattcagac
     6541 caatgatcat aggaatgtct aaatccaata actgcgtagt taaataaaga tttcgttcta
     6601 aattggtagc atcaataatg ttgatgatca gttcaggctg ttcctgaacc aaataatctc
     6661 ttgcaatcac ttcttctggt gtataaggag acaaagaata gatgccgggt aggtcctgaa
     6721 tatgaatgtt cggatttttt ttcaacgatc cttctttttt ttctactgtt acgcccggcc
     6781 aattccctac atattgattc gaaccagtca agagattgaa caagctagtt ttcccgctgt
     6841 ttggatttcc gactaacgcg atattcattc tttttcctcc ctttgtatca agattagttc
     6901 ggcctctgac tttcgtaacg tcagttcata ccctcgcact gtcagttcga ttggatctcc
     6961 cagaggggct agttttcgga taactatttt tacatttcgc gtcaatccca tatccattaa
     7021 ccgtcgcttc actgcgcctt gtccgtggat tgctacgacg atcccagtct ctcctgcttt
     7081 taattgatca agagaagtcc agctttcaga ggaagcagtc tttttattga caaagatttg
     7141 acctagtatt tcccgactta ctgccactcg atgacttttt accagtaaaa tggcattgtt
     7201 gcctgataaa ttgacgacga cgattttact tccggcaacg atcccgatat tttgcaaatg
     7261 gcgttttgtt ttttcttctg tttcgattcg ttcgacttga taaacgaaat tctgctttgc
     7321 ctgcgctaag gtctgcatat tgattcctct cttgtgaaat aaatcatgta ttaaaatcat
     7381 tcattatatg ttctaatttt cttacgtgtt acgcaaataa atgagaatca ttctcacata
     7441 gaatactctc ggtaattgag aatgtcaatt atacttgtga tattatgagc gaatctttag
     7501 tattgataga taaaaatgag aaaaaaatat ttcttcatag ttttccacaa ttttttctct
     7561 attaaatttg ctattttccc acacgatact tgttttcagc tcttttttca aaaacacaaa
     7621 acttagtatt ccacagtttc cacaacacaa tatgtagtgt ttgttgtttg acttttttac
     7681 taagagtttt atactatcta ttatagataa taagaatgtg ttagaggagg cactcaccat
     7741 gaacgtaaaa atcttttcta aaaataattg tatgcaatgt aagatggtca aacggttttt
     7801 aacagaaaat caaattgctt ttgaagaagt caatatcgat gaacagcctg aagcagtaga
     7861 ctggttgaaa gcacaaggat tccaaagtgt tcctgtcatt acttctgatg cagaaacagt
     7921 tatcggattc cgtcctgatc agttgcgtaa acttgccagc taacatgact aaaagaatca
     7981 aaacgtgata catggatttg aagggcttga gacagcacca gtttttgtcg caaacttcca
     8041 aatccttttt taaagcttaa aaacaatgca atgatttgca atcaagcatc atcaaatacg
     8101 aaatgaaggt tgggcgtacg tatgagtctt aaaaatttac aagatgtcag ttatttcaag
     8161 ctgaataatg aaatcaatcg gccagtcaat ggtcaaatcc ctttgaataa ggataaagaa
     8221 gctttagttg ccttttttga ggaaaatgtc aaaccaaata cgatgacttt tctatctatt
     8281 atggaaaaaa tcaattactt gatcgatcaa gaatatatag aagcagaatt cattcaattg
     8341 tattcaccat catttattga aaagctatat caatttttgg acgagcaaca tttttctttc
     8401 aaatctttta tggctgctta caaattttat tctcaatatg cgttgaaaac caacgacggt
     8461 tccgcttatc tggaaactta tgaagatcga gtagccttca atgcgcttta ctttgcgaac
     8521 ggggatgaag aattagcact tgctcttgca gacgaaatga tccatcaacg atatcagcca
     8581 gctacacctt ctttcttgaa tgctggacgt aaacgtcggg gcgaattggt gtcttgtttc
     8641 ttgactcaag tcactgacga tatgaactct atcgggcgct caatcaactc tgctttgcaa
     8701 ttgtcccgta ttggtggcgg cgtagggatt actttatcaa atcttcgtga agccggcgct
     8761 cctatcaaag gatatgaggg tgctgcaagt ggtgtcgttc cagtcatgaa attattcgaa
     8821 gatagtttca gctattctaa tcaattaggt cagcgtcaag gagctggcgt ggtttatttg
     8881 aacgttttcc atccagacat tattgctttc ctttcagcaa aaaaagaaaa tgcagatgaa
     8941 aagattcgcg taaaaactct ttctcttggc gtgatcgtac ctgataaatt ttatgaattg
     9001 acacgtaaaa acgaagatat gtatttattt agtccatata gtgtcgaaaa agaatatggt
     9061 gtcccattct cttatgtaaa tattacagct gagtatgatc atctagttgc aaatccaaac
     9121 attcgcaaac aaaaaatcaa agcgcgtgat cttgaaaatg aaatctcaaa attacaacaa
     9181 gaatctggct atccttacgt aatcaatatt gatacagcga accgcgagaa tccaatcgat
     9241 ggcaagatca tcatgagtaa tttatgttca gagattttac aagttcaaac gccttctgtg
     9301 attaacggca aacaggaata tgaagtatta ggaacggata tctcatgtaa cttaggctcg
     9361 acaaatatcg tgaatttgat gcaaagccct aactttggtc aatctgtgcg tacgatgact
     9421 cgtgcattga catttattac agatgcatct gatatcgatg tcgtaccacc gatccaaaac
     9481 ggaaataagc tgaatcatac gattggatta ggtgccatgg gcttacacac ttatcttgca
     9541 aaagaacaaa tggaatatgg ctcagaggat tcattagact ttacaaatat ttacttcatg
     9601 ctgttgaatt attggacact tgttgaaagc aacaatatcg ctcgtgaaag aaaacaagtt
     9661 ttccataatt ttgaaaaatc agcttatgct gacggcactt atttcgaaaa atacgtaact
     9721 ggagaattcc agccaaaatc agaaaaagtc aaagcattgt ttgaaggcat ttttatccca
     9781 actgctgaag attggaatgc tttgaaacaa gcagtaatga aagatggact gtatcatcaa
     9841 aatcgcttgg cagtagcacc aaatggctca atttcatata tcaacgatac gagtgcaagt
     9901 attcatccta tcactcgcat gatcgaagaa cgccaagaga agaaaatcgg taaaatttat
     9961 tacccagcgc cttatctatc caatgagacg attccgtatt acacttctgc ttatgatatg
    10021 gacatgcgta aagtaatcga tgtttatgca atggcacaac aacacgtcga tcaaggcatg
    10081 agtttgactt tattcatgcg ttctgaaatc ccagaaggca tttatgaatg gaagacaaca
    10141 acgaagcaaa cgacacgtga tttgaatatt ttaagacatt acgctttcca caaaggaatc
    10201 aaatccattt attacgtacg tacctttact gatgatgcag aagaaatcgg aagcaatcaa
    10261 tgtgaaagct gcgtgatcta atatcataga tagattcgct tattcatatt ttaattaaag
    10321 gagcattctc atgtcagaaa catattatga agcaataaac tggaatgaaa ttgaagatat
    10381 cattgataaa tccacttggg agaagctgac agagcaattt tggctagata cacgtatccc
    10441 actttcaaat gacctagatg attggcgtac attgtctcaa ttggaaaaag atacagtagg
    10501 acatgtattt ggcggtttga cactgctaga tactgtccaa tctgaaagtg gcatggatca
    10561 gttgcgtaaa gatgtccgca ctccccatga agaagccgtt ttgaacaata ttcaattcat
    10621 ggaatccgtt catgccaaaa gttattcttc tatttttagt acactgaata cgaaaaaaga
    10681 aatcgaagaa atttttgact ggacaaatac gaatccttat ctgcaaaaaa aagctgagcg
    10741 tatcaatgaa atctacaaac acggtacacc gctagaaaag aaaattgcca gcgtcttttt
    10801 agaaacattc ttgttctatt caggttttta tactcctctt tattatttag gaaacaacaa
    10861 attggctaac gtagcagaga tcatcaaact aatcatccga gatgaatctg tacatggaac
    10921 ctacatcggt tacaaattcc aattaggatt caacgagtta cctgaagcag aacaagaaac
    10981 gttaaaagac tggatgtaca atcttcttta tgagctttac gaaaacgaag aacgttatac
    11041 agaagaatta tacgatccaa tcggctggac agaagaagtc aagacattcc tacgctataa
    11101 tgccaacaaa gcattgatga acctagggat ggatccactc tttccagata ctgccaatga
    11161 tgtcaaccca atcgtaatga acgggatttc aacaggcaca agcaaccatg acttcttctc
    11221 acaagtcgga aacggctatt tgttaggtca tgtagaagcg atgaaagacg acgattattt
    11281 gattggattg gaataaggat ttattttaat tcaaaaattt ttcgaaaaat aaaaagagtt
    11341 tagcgtacag atattttaaa tgtacactga actctttttt atgcttattc acttgtttct
    11401 tccagcactt ttggactgtc taacttttcc gaaacatgtt ccattcttct caataaatag
    11461 attttccgga tatttaatat tatcgtctta atcactgcat agacaggtat gcaaatcagc
    11521 atgcctaata ttcctgcgac acttcctgct ccaatcaata ccaagatgat cgtcaaagga
    11581 tggatattca ttgatttccc aaataacaac ggcttgatca aatttccatc taattgttgg
    11641 atcactaaaa tcgacaacgc catatacaaa gcttgaaatg gagagataaa caaaccgaca
    11701 atgattgctg gtaccgctcc aataaaaggg ccgacatacg ggataatatt cgtgattccg
    11761 caaatgaccg caagcaacaa gctataaggc tgatcgaata ttctcattgc caaaaagctc
    11821 attacaccaa taatcaaagc atctaagatc gtactgctga tataggcaga taatgtttcg
    11881 tttaattctt ttaccgtttg gcgtaactct ttacgaatcg ctttcggaaa gaagtgagac
    11941 aacgcatcaa taaatttgtg cccatccttg aacataaata gcaaaataaa tggaacggta
    12001 aataacaaaa taaaaaattg catcataaat gaaatgattt ttgagacact tgcagataaa
    12061 ctgactaata tgatattgaa caaattacta agagataaat ttgctgatgc taattgttct
    12121 tccaaattga aatttttaaa ctcttcccgc tgcgccaatt catttaacca tttgcttgtt
    12181 tcatctgcat acattggcaa gctctgggtc aattgaattg tctgttccat taactgaggc
    12241 actacattca tgacagttaa aacaactaaa atgattacga ccaaaaacgt caataaaaaa
    12301 ccgaacactc tcgggatccg atgcttttcg agaaaaacaa cgattggatc gaacatataa
    12361 tagataaaac cagcaacaag caatggtaaa aaaactgccg aaagcatttt caatattgga
    12421 ctaaaaatat tcggcatttg taaaataaag tacacgccga tgatcgtgag tactagctcc
    12481 actgtccaga aaaataattt tgattgttta aaacgtgatt gcatacatct cctccaaggt
    12541 cctttgatac ttctattcta tcatttaaca aagagttacg cgctgagatt ttcatttgct
    12601 taaaaaaaac aaaaaaaata aacatatttt aatgagtaga cgagtactta aacaaaaaca
    12661 cggtttggag tagtgacaaa aattatactg cagtcccttt ttccaaataa acggtgttca
    12721 aaagcttgca tcttcgtaat aagcccagcc aaacgaaaaa tataaggaac tatttttatt
    12781 tggctgggct tattacttga tgtaggacat ctattttaca acctcagatc atttattgcg
    12841 caatactgac taaatgcaac tttgtgatat ttttgtgtac ggacaactcc tcgatcacat
    12901 cggcataagt gagccctttt ggaagatcaa tcgtatagat atttttgtac actcggtagt
    12961 catcaacgaa ttttacgtca aaattcacat cttcgattac gatatttttc tcttcaaaat
    13021 aatcggtaag aaaatctttg gtttcttttc tatggataaa ttggatttct aactttttag
    13081 tcgtaggtac atggatcacg cgtttcacta ctgtgagtgc aatagcaatc ccgataaaac
    13141 tgatgatcgc aatggaatag taacccatgc cgatggcgat ccctaatcca gcaattgccc
    13201 ataaagaagc ggcagtcgta agtcccgtca ccgattgttt cgtgacgatg atcgttcccg
    13261 cgcctaagaa accgacacca ctcaccactt gagcaatcag ccttgcttca tctgaacgaa
    13321 tcgttccagc aagctgtgga tactctaaag catctcttaa tgcaccacta gcgatctcaa
    13381 cttggatcaa tgcaatgata catgctccca tacaaactaa aatatgcgta cgcatacctg
    13441 ctggacgatt tttatattgt cgttcaaaac cgattgctcc gccaaatagc atagcgatcc
    13501 ctaaacgtaa aataatctct ggaattgtca ataccatatc attcatctaa acaccctcca
    13561 gtccaatata tcatgtctga aaggagcaga caaaaaaagt gcaattttta cctcttacga
    13621 aaaaaggact gtgacaaaat ttttgtcata gtccttttta tgaataaacg gtgttcaatc
    13681 agctgaccct cggtattact caacaaacgg caaaatatgg ggagctattt ttcctttgtc
    13741 ttctctaata ctcaggtcaa aacgctgatt tcacaacctc tttagcctac tattgaaaat
    13801 tacaacttac ttgctgcttc ttcatcgatg atcacaacaa cattgtcgtg attttgcaat
    13861 acacttgctg gcaaatgatt cgttacaggt ccttcaatca tccctttgat tgcatcagct
    13921 ttgctttcgc caaatgccat caagacgatt tctttacttt tcataattga accgattccc
    13981 attgaaacag cttttgttgg tacatcttct gctttatcga agaatcgttt gtttgcattg
    14041 attgttgatt ctgttaagtc aactactgaa gtcgttccgt caaatgaagc accaggttca
    14101 ttgaaaccga tatgtccatt ttgcccgatt cctaagattt ggatatcgat tggatgtgca
    14161 tcgatgattt gatcatactt tttgcatgct gcttccagat cttctgcttt tccatctggt
    14221 acaaacgttt ctttgaatgg ttttttatca aataattgga catccatgaa atgacggtag
    14281 ctttgttcat ctgaaccgcc tagtccaacg tactcatcta agtttacaga agtcatttca
    14341 ctgaaatcca aatcgcttga agtcatttct ttatataaag taactggtgt gcttcctgtt
    14401 gccaatccta atacttttgc acctttgttc ataccttctt tgatgatttc aaatgctttt
    14461 tttccgcctt cttcagcgtt ttttacacga atgatttcca tagtgattgc tcctttatat
    14521 tttggtatag cccaattata gcattctttt tttcttttta caataattag tatagaccaa
    14581 attgcatttt tttcattttc ttgacaataa ataggaatcc gtgttaggtt tcataagatg
    14641 gttcaatcaa acaatccatg ttgcatgaaa accaagcaag tgattcactg tggacgcttt
    14701 atgtctgcag tttttttatg aaaaaggagt tattatgagt atactgacat taaacaacat
    14761 tacccagcaa tttggcgaca aaatcctcta tgaaggtgtc gatctgcaat taaatgctgg
    14821 cgaacatctt gggctgattg gacaaaacgg agccggtaaa agtacattga ttaaaatcat
    14881 cactggtgag atcttaccag atgacggaca gatccattgg caaaaaaata ttcacaaagg
    14941 ttacctcgat caatatgtgg aagtcgatga aacattaaca atcgaagaat ttctaaaaac
    15001 tgcgtacgca aaagaatacg aaaaggaaga aaaaattgct tttctttacc aaacatatag
    15061 cgagaccttc gatgattcct tgctggaaaa agctgggaaa ctccaaacgg aattagacca
    15121 aggagtgttt tatcaaatcg acaccttagt tgctgagatg agcagcgggt tggggatcga
    15181 tgtcttaggt ttagattcac tgttgaaaaa tctaagtggt ggacaacgtt caaaggtgat
    15241 tttagcaaaa ctacttttag aaaacccaga tgttctgatc ttagatgaac caaccaatca
    15301 cttagatgat cagcatatcc aatggctgac gcagtttctt caggattttg acggtgcgta
    15361 tatcgtgatt tcccatgacc aagaatttct tgatcagatc accacccata tcgcagacat
    15421 cgagtttgga aagattacta agtatactgg ccatctaaaa caagcattga aacaaaagga
    15481 acaaaatcgc gaaagctatt tgcgccaata tcatgctcaa caaaaacaaa tcgaaaagac
    15541 cgaagcttat attcgaaaat acaaagccgg ttcccgatca acgatggcaa aaagccgaca
    15601 aaaacaatta gataaaatcg aacggctgac gcctccagct tcagcaagta aagctgtctt
    15661 tgattttcca ttctcgccta ttgtcacgac attagcagtg gaaacaactg atttagtcat
    15721 cggatatgat cgaccgctac ttgcacccat caatttgacc atccgctttg gtgaaaatat
    15781 tgccattcga ggcttcaacg ggattggaaa atctacttta ttaaaaacac tgatcggtga
    15841 gattgaaaaa ataagtggcg atttccactt cccagataac acaaaaatca attacttttc
    15901 acaagatctg tactgggaaa atccattaga aacaccacta caatatttaa gcagtcaatt
    15961 tccaaaagcg actatcaaag aattacgtcg ccaactagct aaagcagggt tagttaatca
    16021 attagcaagt gaacctctca ctacattaag tggtggggaa caaactaagg tgaaacttgc
    16081 acagttgaca atgaaccaag gtaatttact gattctcgat gaaccgacga accatatcga
    16141 tcaagaaacg aaagaaagct tacaagacgg gatacagaaa tttccaggaa cagtaatcat
    16201 cgtctctcat gaacaagaat tttatcaaga tcttgtgcaa cgagtcattg agattgaacc
    16261 aaaataaacg tttgattgaa tagttgaaaa aaaggagctg tgacaaaagt tttgtcacaa
    16321 ctccttcttc tgaataaatg gtggccaatt agctgacctt cggtattagc tcaaaaaacg
    16381 gcaaaatatg aggagctatt tttatttggc tattcttatt acttgatgca gaacagcttt
    16441 tcacaacctc ttttcgtcta ttttttattt tcgagatttc tctttccttt aaaaacaact
    16501 gtttgaaaga agaacgcaca tccttgaaaa acaagcccgc taaccgttac acctaaccag
    16561 ccatactgct gccacattgt cgtccctagc aatgaaccag ccgcacctcc aataaagtac
    16621 ataaacataa atacagtatt atttcggttg cttgctgctt cacctagccc ttgtactcgt
    16681 gtttgattgg cgacttgacc aaattgattc ccgacgtcta ataaaataat agctaaaagc
    16741 aaaatggcca aatggcttcc gccaaccaat aaagcaataa agcttattgt ttgcataaca
    16801 agtcccatat acacgatttt tctttcagaa taagaatctg ccagtcgtcc cacgataggt
    16861 gcagcaactg ctccagacaa accaaaaatc gctaatatcc ctgcttcgaa tgttccccaa
    16921 ttgtaggcgg gactgctgat ataaaaaatc aacgtagacc aaaagatcga aaatgttcca
    16981 aacatgaaaa atccactgac tgccgcttct cttaatagac gttgagaagc aactaaatgc
    17041 ggcaaacttt taagtgaatc aaggtatgaa acagctgtac tactttgctg gacagaacgc
    17101 ggcattttcc agtaaagcag aagtgtaaga attccgactg cacataaagc aaataaatag
    17161 atcatccgcc aagaaaaaag actggcaaca ataccagata ccgtccgaga aagcaaaatc
    17221 cccacgagta aaccgctaag caatgtaccc atgaccttcc ctcttgcttt tggtcccgct
    17281 agtaccgcac cgtaaggaat gatgatttgc ggcacgatcg aaagtaatcc tattaaaaaa
    17341 gaagcgcatg caaataacgt aaacgaaggc gcaaaaaaag cggccaacag agagacggca
    17401 gataacgctg ccattcgaat gatcaatttt ggacgatcca ccacgtcccc taaaggaact
    17461 aaaaacagta accctaacgc atagcctaat tgggtcaaca tcgtcaatat cccgactgca
    17521 cttgtgctaa cagataaact agctgctact tttgttccaa tcggctggat gtaatacata
    17581 ttggcaacaa caacgccaca agtgattgat aatagtaaaa taagcgaagt tgttaaaggt
    17641 tttttttcat tactcttcat tgatttcccc catttcctat cttagtttac aacttatgga
    17701 aaagattgga aatagaaaaa tacaactatt cgccacactt attatttgtg cttttgtttt
    17761 ccttgttttt tcttgcgatc tttataaatg aagacaacta accctactag cacagtaaca
    17821 cttgcaacca acgtgactgc agttttgtta tatccgtgaa acagagcttc tccaccacac
    17881 gcatacaaca cagacgaagg gatgactcca atcacgatcg acaatatgat ttgctgtaga
    17941 ggcaatttca tcgcatctgc agcaaaattg accatagaag atggaataac cggtatcata
    18001 tagcctaaca ttacacctat ttttggatgt ttcatttgtc ggattgcttg cacccaatga
    18061 ttcgtttcgg ttttcttatc taaaaatttt aagcgatgca taaggaaaat tgcagtgatg
    18121 ttaccaagag cattacccaa aatgttgatg atcgcacctg tcagtggtcc aaagctcaaa
    18181 ccagcaatga ctccaactac tgaagtagga atacctggaa cagcacacat caccgctgtc
    18241 aatacgatca agagtatcga tgcaaaagtc ccatgcgaac gaatatgtgc gagtagctct
    18301 gccttactcg ctctcggatt taaaaataat tggatgtctg tctgatattc taaatacaac
    18361 cgatagccca ctaccagtat cagaatgatt cccaacgaga tcaatatcaa tcggattatt
    18421 ttctttttca tatttcctcc tgaaaaaaat ctatattcat taaataatag tttaacataa
    18481 aaacaaaaaa gcaccgaaat ccaacatagg atctcagtgc ttttaaatag taaaaattat
    18541 ttaacagtta cagatgcgcc aacttcttca agtttagctt tcaattcttc agcttcttct
    18601 ttagatacgc cttctttaac tggtgcagga gcgccatcaa ctacagcttt agcttctttc
    18661 aagcctaaac cagttgcttc acgaactgct ttgataactt taactttttg gtctccagct
    18721 gcagttaatt ctacagtgaa ttctgtttgt tcttcagcag cagcagcgcc accagcagca
    18781 gctacagcta caggagcagc agcagatacg ccaaattctt cttcaatagc tttaactagg
    18841 tcgttaagtt ctaggatagt tgcttctttt aactcagcaa cgatgttttc aatgttcaat
    18901 gccattataa tttcctccgt ttaattgttc ttttttattt aatgctttat gcggcaatta
    18961 agctgcatct ccatcttctt tgtctgccac tgctttgaca gcgtaagcca cgttgcggac
    19021 tggcgcttgt agtacagata gcaacataga tagaagtcct tcgcgatttg gcaattttgc
    19081 caatgctgtg atttcttcca atgaagagac tttaccttcg atgataccgc ctttgatttc
    19141 taatgctttt gcttctttag caaattcgtc catgattttc gctggtgcga ctacatcttc
    19201 gttactgaat gcaacagctg taggtccagt gaatacttcg tccatgcctt caaggccagc
    19261 ttttttagct gcacgtgaaa gaatagaatt tttgataact ttcatttcta cgttagcatc
    19321 acgaagttgt ttacgtaaac gtgttacttc atcaactgtt aatccgcggt agtcaacgac
    19381 tactactgat gcagctgctg taaatttttc gcttacttca tcaacgattg ctgctttttt
    19441 tgcgataatt gcttcactca tttttgagtt tcacctcctg aattttgatg gaagagtttt
    19501 ttgcactgaa aaggcaagtc acttttatag ttactttgct caaccagtat tattcatcac
    19561 ctattcactt cgttcatagt gattctactc gctttgctcg aaaggaaact cctttcgctt
    19621 tgctcaacca gtattgttca tcatctcttc acttcgttca tagtgattca caataaaaaa
    19681 ctctatgcca ccgcagacat agagggacta ttgaatatat tcaataaacg tcctcggtag
    19741 gaaattaagg cttgatgcca cctactgtct tcggtacaag ttaattatta acctcaacta
    19801 gcttagcatg ctaaactagt tgagtcaaga attatttttc agttggatca atcaacattc
    19861 taatatttaa ttagaaagaa gcttggtcaa catggatacc aggtccaaat gttgttgtga
    19921 tcgttacatt tttcatgtat tgacctttag ctgctgatgg tttagctttt agcaatacat
    19981 catggattgc tgcaaagttt tcaactaatt tttcatcgtc aaatgaaact tttccgattg
    20041 gtacatggat atttccagct ttgtccacac ggtaagttac tttaccagct ttcacttcgt
    20101 ttactgcttt tgttacgtcc attgtaactg taccagtttt agggtttggc attaagcctt
    20161 ttggtcctaa tactcgtcct agacgaccta cagtagccat catatcaggt gttgcaacaa
    20221 ctacatcaaa gtcaaaccat ccgttttgga ttttttgaac catgtcgtcg tcacctacga
    20281 aatctgcacc agctgcttca gcttcttttg ctttgtctcc tttagcaaaa actaaaacag
    20341 tttgtgtttt accagttccg tttggtaata caacagcacc acggatttgt tggtctgctt
    20401 ttttagggtc tacgttcaaa cggtatgcaa cttcaactgt tgcatcaaat tttgcaatgt
    20461 ttgtttcttt tgctaaagct actgcttcgg caacaggata aactttactt gaatcaactt
    20521 tttttaatgc atcttgcatt tttttgctct ttttagccat tttggttcct ccttgattgt
    20581 ggttataacg gttgtacctc ccacgtgttc tatatctttc aatatagagc caactgagaa
    20641 gctgctgtct tagggtgtat agattattct acagtgatcc ccatgcttcg tgcagttcct
    20701 tcaaccatgc gcattgctgc ttcaacgtta gctgcgttta ggtcttccat ttttaattca
    20761 gcgatttctc ttacttgatc gctagaaact ttcgcaactt tatttttgtt tggttcgcct
    20821 gaaccttttt cgattttagc tgcttttttc aatagaacag cagctggcgg tgtttttgta
    20881 acgaatgtga atgaacggtc ttcgtataca gaaataacaa ccggaatgat caaacctgct
    20941 tgatcagctg tacgagcgtt gaattctttt gtgaatccca tgatattgat acccgcttga
    21001 cctagtgcag gacctactgg tggagctgga gttgctttac ctgcaggaat ttgcaattta
    21061 acgatttttt ctactttctt tgccacgaga catacctcct taagtccgtg atgtggttaa
    21121 ttggagatgc atatttctcc tcccacgttt tatctgatct gaaaaaatcc agacaaataa
    21181 tgtgcaaaac aatgcacact ggtacagtat agcaaattta tttgtttttt caagattttt
    21241 ttctgctatt tttacatttt gcttgaataa acgctaaatt tattctaaaa aaacaacaat
    21301 ttttcgttct catttcttct tttatgttac acttgcgcta gcatggttca acagatgaaa
    21361 gcaaacaata acacggtttt ttagaccctt gtacaaagat tcctacataa caaaatgagg
    21421 tggagaaaat gaaagattta aggtatagaa gtgtctttga tattattgga cctgtgatga
    21481 ttggtccaag tagctctcac actgccggag ctgctagaat cgggaaaatc atacgtagta
    21541 tatttggcga acagcctgat tctgtagata tttatctata tgaatcattt gcaaaaacct
    21601 atcgggggca cggaacagat attgcattag ttggcggcct gttagggatg gaacccgatg
    21661 atgaacgttt agctgattct ttgaaattgg catatgaagc cggtatggaa gtcttgtttg
    21721 ttcctaagag tgaaaaagcc gaacatccaa actctgtaaa gatgcttcta aaaaaaggaa
    21781 cacgaaagct ttcagtcact ggcatttcga tcggcggtgg caatatccag atttctgaat
    21841 tgaatggatt caaaatttct ttaagcatgg gtactcctac cttgatcgtt gtccaccaag
    21901 atgttccagg tatgattgct aaagtgacta gtatactttc tgagaccaat acgaatatcg
    21961 gcactatgac agtgacacgg gaatcaaaag gtgaaaatgc cattatgata attgaagtag
    22021 atgatcctca agtagaagaa acggtaaaaa gactcaaaca gctgccaaat atcgacagcg
    22081 taaattattt cgattaagga gctaaaaaaa tgtttttatc gattgaagaa ttagtagatc
    22141 aagcaaaagc ttatccaagt gtagcagaat tgatgattgc agttgaaatg gaaatgagtg
    22201 gccgttcccg tagtcacatc atcgagatca tggaaaaaaa cctctcagta atggaacaat
    22261 caatcaacga gggaaaagaa ggtgttacct ccgtcacagg aattaccggt ggtgatgcaa
    22321 aaaaattaga tgattattta aatgaggcag actttttaag tggagaaaca atcttaaaag
    22381 ctgtacgcaa tgctgtagct gttaatgaag tcaatgcaaa aatggggttg atttgtgcta
    22441 caccgactgc cggaagcgcg ggtgttgttc ctggtgtgct gatggctgtc cgagatcggc
    22501 ttgatttgac acatgaacag caattggaat tcttatttac cgctggcgct ttcgggttag
    22561 tcatcgcaaa caacgcttct atcagtggtg cagaaggggg atgtcaagca gaagttggtt
    22621 ctgcaagtgc aatggcagca gcagctttgg tttgtgcaaa aaatgggact cctcatcaag
    22681 cagcacaagc agtagccatt acattgaaaa atatgatggg actgatttgc gatcccgttg
    22741 caggcttagt tgaagtcccg tgtgtcaaac gtaatgcttt aggctcttct caagctttta
    22801 tctctgctga tatggcatta gcgggtatcg aaagtgtcat tccgccggat gaagtcgttg
    22861 ctgcaatgta ccaagtcgga cgtcaaatgc cgtcgatttt caaagaaaca gcagaaggtg
    22921 gcttagctgt tacgccaact gctaaacgac ttgcggaaga gattttaaat aagagggcgt
    22981 gataaagctc cttaatttcg agtattaaga gattagttat cttcataaca aaaaacgaag
    23041 agaggactaa ttgcccttct cttcgttttt tattaatcca tcaatttcgt attttttact
    23101 tcatcgatcg tttttgttcc tgcaagttgc atcgtgatcg acaattcttt gttcaggtgt
    23161 tcaaaaacag atgttactcc ttctgcccca cctagattca acccataaat cactggtcgg
    23221 ccaatcgcaa ctaagtcagc accacttgcc agagctttga agacatgctc tcctcgacgg
    23281 acaccagagt cgaaaatcac tggtacacgt ttgttgacta cttcggcaat ttttggcaat
    23341 acttcaaagg acgcaggacc tccatctagt tgacgtccac catgattgga tacccagata
    23401 ccatctgccc ctgcagagat agcgattact gcatcttctg gcgattgtat tccttttacg
    23461 ataactggaa gatgggtgat ttctttgatt gttttgatgt catctggtgt caagccttgt
    23521 tttgcagctg cataaatttc tgcgattcct tttccttcgc cattaccact tgcactttgt
    23581 tcgctatacg cagctaagtt aggcattggt aatggaaatt ggaattgatt gatgacgtct
    23641 tcttcacggt aaccaccgag cgttgaatct gccgttaaaa tgatagcttt agcccctgct
    23701 tctactgctt tgttcaaaat gaactcattg aatctgtcgt ctttactcat atataattgg
    23761 aaaaactgcg gtgcgcctgg cgctgcatcg gctgcgtctt tgattgtcgt atttgcataa
    23821 gtgctgatgg aaaagattga tccagcagca gctactcctt tagctgtatc agcttcccct
    23881 ttttcatgtg ccaatccttg ggccgctgat ggcgcttgga taatcggtgt atctaattca
    23941 attccgaaaa cagatgtatg caaatcggct gagtcgattc cgcgtaatat ccgtggcatg
    24001 attttctttg tattaaaaga agtcgtattt tctttcatcg tccactcatc ttcagaacca
    24061 ccgcgaatat agccaaaagc tcctgcttcc atgcgctctt ttacacgacc ttctagttag
    24121 gcaatgttta caatatcgat tggatgttct tcattacttg tatgataagt catagtagat
    24181 ccttctttca tcttcttgat tacatataaa tcatactaaa cttacaaaaa gtaagcaaac
    24241 atttactctc agatactttt atcgctattc tttccaaata aatcgactta caagctgatc
    24301 tacttcttgg ttttcatgaa gcatactatg agaggcattt tcacctgtca ctactttttc
    24361 ctctatttca tttccgcgtt gcttcaacaa cgcatagacc gctaatgcac tgtttaacgg
    24421 tactgttcca tcactcaagt ctgtttcatc tagttgacca gcaatcaaga aaaatcgagt
    24481 ggttatagga atattaccga tcatttgctg gtaatcttga tagcggttag aaacgacagc
    24541 aggccccttt gctaactcat ctgtcagaga ctgcgcacta tcatcgacaa aatcattaaa
    24601 aggcgcgcca attgctgtta atgtctgaac ttgtggcaat gacgtatcct gtccatacgt
    24661 tcctaaatag cgtagtgcac taactcctcc catggaatgc cccactatgt tgactttatc
    24721 tatgccgtag ttttctttca gatacaaaag acaagttttg atccattctg cttgattcca
    24781 ctcgttattt ttattatctt caaacaatac ttggaccatc ggattgtctg cttttccaga
    24841 caaatctcct gttgcttgta tttgtccatc tgttcctact gttaaaacca attcttttac
    24901 tgcttgctga tttttttcca aacgccgcaa catcccttta aaggaatttt ctgtcccgct
    24961 gtagccgtgg aaaaaaagtg ttggaatagt ggttttttct atcactttac tggattctgc
    25021 cgttttttct ttatcagaaa cttcagtagt atttatcttc gtcgtctggc aaccacttaa
    25081 cgcaaacaaa ctgataagga ctaaagctgc tgcagttgca cgtttgcata aatttttgat
    25141 tggttctgtc atacacctct tcctttccat atagaaaaaa gaaagacttg aaaggccaat
    25201 catatgatcg acatttcaag tctctttatt aatcgatttt atctacttga tcgaaatcaa
    25261 gttctgtact ggtttcacgt ccaaacatat cgatgttgac tttcaatttt tctttttctc
    25321 cgtcaacttc tgttacttgt ccttctaatc cagcaaatgc gccttcgatg attttcacga
    25381 cttcgccaac ttctacttct agatcatttt gtcgcaggct cataccgata gaacgcaaga
    25441 tatgattgac ttcttctgga agtaatggtg ctggtttgct tccggctccg tgtgaaccaa
    25501 cgaatcctgt cacaccaggt gtgttgcgga caacatacca tgaagcgtct gtcatgatca
    25561 tttcaacaag tacgtatcct gggaatgttt tatgaacgat ttctttttct ttcccatttt
    25621 tgacttcagt ttctttttct tcaggaacaa cgacacggaa aataaagtcg gccattttca
    25681 tactttgcgc acgagattcg atatttgctt ttactttgtt ttcatatcct gaatatgtat
    25741 gcaagacata ccagtttttt tcaaaagatt ccattgaagg ctcctcattt cattgtaaaa
    25801 taaaaaaacc tcagtacacc cgaagttttt ctttcgcttc attatagcac taaaagtacg
    25861 ttgtggctag cgagttattt taaaatccag ccaaataagc tttggatggc tgtatccatt
    25921 acgtaaaaca tcaatccgaa aataatagaa gtttcgatga ctactaacgt atctttacgg
    25981 agttgttttc tgcttggcca agttacttgt ttgatttcat cttttacgct ttttagaaat
    26041 ttcatgagac aacctccctt gctactgttt atttcgtttc tttatgtaat gtatattgat
    26101 tacaatattt acaaaactta ttgatttcca aacgttcgcc gctttttcct tcactgactg
    26161 attttgtgta gttgcgagac ccacaaacgg aacaagcgag tgctgctttt ttctttgcca
    26221 tttacttctg cccccttgct gagaatcaga cttatacttc ataacagtat cacgtaactt
    26281 tttacttgtc aatttgtgaa ataaactatc tattgttttg gataaaatta tgatatgatt
    26341 aatttgaaat taagctaaaa tgagacaatt ggaggtatag cgatgctatt aaaaacactt
    26401 gtatataaga aaaaagatct gacaaccgtt acagagacta gtacattaga agaagcatta
    26461 aaaattttag aaacttccgg ataccgttgt gtgccgatct tagattcttc aggtaatatt
    26521 ttccgtggaa acatttacaa aatgcatatc tatcgtcata aagcaaatgg cggtgatatg
    26581 acgcttcctg tcactcatct tttgaaaaac gctacaaagt tcatttacct ggatacttct
    26641 ttcttcaaag tattctttac gatcaaagag cttccttata ttgctgtcct tgacagtgaa
    26701 aatcattttt atggtatttt gacacatagt acgttgctta atatgctctc tcaatcttgg
    26761 agcgtagaac aaggaagcta tgtactgaca attgcttcca caggaaagca aggagatttg
    26821 gctgctattg caaaaatcat ttcaaaacac agcagcattg ccagctgtat tactcttgat
    26881 atcgaaaaag acgaatacat tcgacgtaca ttgatcacgc taccggctaa cactgaagcg
    26941 aaagtatgtg aaacgatcgt caaccactta gagaaaaaga attataaagt ggtagaaatt
    27001 gaaaatttgc aaaaccaaga agcataatca aaaagcgaaa aaagaaaaca gctaatctgc
    27061 tttctttttt cgctttttat aattagacac gcttttcaaa atcaagcgcg cgatctaaat
    27121 aatacatcaa tggtgctgaa acagccatga cgataaactc acttaatgca agcgtgccat
    27181 aggttgtcca aaatgcttgg ccacctgttg gaactaacat ataagcaatc agtcccatgc
    27241 tgatggtaaa gaagatcgtg ttcaatacta aacgcatctt tacatttttc actttgtttt
    27301 gcaataaagc tgtcaggcct aaagaaagca atgtttggcc gccaccaaaa accacatcca
    27361 tgataccaaa accaaagaag gcattataga cgatgacccc gcctaaaact ccccaaagca
    27421 atttacggtt aaagacaact aaatgattca aactttctga aatacgaaac tggatcggcc
    27481 ctgaggatac aggcgcaact aaaatagtta acgctagata aagagccatg atgatgccat
    27541 ttacaacgat tacggataac cgttgtctgc ttttcgtttt ttgcattttc tctcctcttt
    27601 tctcctagtt tttttaacgc gggatggttg atgaaccgcg gttatgtgaa acaaacaata
    27661 attataacag acttttcatt tatttcaatc cgtcttatct gcttttaaaa aagcaaaagc
    27721 ttaaacaatg taaacgtttg ctaaaaatcg tgggatatgg tataaagtaa gtgtaagggg
    27781 tcataagaat tcccagtaaa gcagacgaag taggatctgc aagaacagaa ttgttgtgaa
    27841 acttaaataa ataaaaaagg atgtgggaat ttgagcaacc tcttactaga gaacggtcta
    27901 gcaagcagct gaagatctaa gtggacatgc cgaaccttgg aacttctcct tacgtaagta
    27961 atggatcatg cgcttgctag gcttttttgt gtttttcggt cttcggacct ttttcatttt
    28021 cctagacttc tgctataata accaagaatt aattttgtaa cagatttttt tgaaaaaaca
    28081 agtttctttc attcaaaaaa tagaaaaagc ttgctaatta ttggttaacc agcaaacagg
    28141 agtattaaaa tatcttgttt tattgaatag caggaggaaa tcatgaaatt ccattatcat
    28201 atattgatca atccatcggc aggcagcgga aatggctata aagtagcaga aagaatttta
    28261 ccggtattaa aaaataagca tattgactat acacttcatt atagtgaata caaaggacac
    28321 gatgctgaga ttgctgaaac tttggcaaaa gaaacattga ttccttggat agaagaagaa
    28381 cagaaaacaa tcgatacttt cccccttttg atcatcgtag gaggtgacgg caccttacat
    28441 caggtacttg atactttcta tcagatggaa gtagaattcc cagttgctta tattccagca
    28501 ggttctggag atgattttgc tcgaggggca ggccttccaa aaaatcccaa aaaaggactt
    28561 cagctgattt tagcagctca atcaccagaa aaagtccaca taatggctta tgaagaaaaa
    28621 ataagtgaaa aaaaaggaat agctgttaat aatttcggta taggattaga tgcagctatc
    28681 gtccatgcga caaatcattc ttccacaaaa aagcgattga acaaatacaa cttaggatca
    28741 ttttcgtatt tattctctat tctccgtgct ttattcactc aaaaaggatt tccaattctg
    28801 gtagatgcag gaggaaaaca attaagtttt tcaaacgcat ttttatgtac agcaacgaag
    28861 catccttttt ttggcggagg aatcgcgatt gttccaactg ctgatgcgtc taaaccagtc
    28921 attgattttg tcgtggtcga acgaatcaat ttctttaaaa tcttgtggct cattctttta
    28981 cttatacaga aaaaacaatt gaaatcgaaa tatttccatc actacacaac aaacagatta
    29041 cgtatcgttt caacaacacc gcaacatggt caagaagatg gagaagaaat ggaaaaacga
    29101 ccttttgata tcacaataac cacaactaaa cagttgtttt ggtgttaata aaagaggttt
    29161 tgtcagaagc tacatctgga acaccgttta ttcggaaaaa aggactgtga cacgactttt
    29221 gtcacagtcc tttttttact atgcagttaa ttctttgccc aattgggttg ccatcgatag
    29281 tttctttctt tacccaaaat aaattggatc attgaaaaca ccgcagcaaa agcaactaag
    29341 acacaaccat tgaaccaaaa agattctgca aatgtttgat tgtaactgag tgtttcactg
    29401 atcaagttat tattttgaac aaaccaatat gttggtgtga atgccgctat tttgctaacc
    29461 atttctggca agtattctgc tggtacaaac accccaccga taaagcaact gcctgtgata
    29521 aaaatatctt tgatcccacc gctggtttct gagtttttta ccaatgaagt gatacaagcc
    29581 gaaaagctaa tgatcggcaa taaaaacaag aaagtattta aaatatgata ccataccgtt
    29641 atatcccacg aggtgtggat cactaacaga accattagaa gtaagaagcc actgaacaac
    29701 agcagcgagt aactaatcaa actaaaggtt actttgcgat tcaagtttcg tttggtaatg
    29761 ggcgcactct ggtttcgttt actgatctct ttgcgattaa atgctagatt gatgacgctg
    29821 aaaccgctaa aaatggatag aaacaaccca taagacaaga aattaaaggt tgtgcccgtc
    29881 aacagctttt tcatccgctg actatatccc gcacttagct caacttctcc agtaaccgag
    29941 agtgccattt gtgtctgttc catagctgca tcgattgttt tatcttcttc ttgaaacaga
    30001 atcagtgttt gcaaaaaggt gttcacttgc tgtgtaacaa ctgttttaga aaaggcatct
    30061 gggcgactct gtatcttgat cgtcggcatc tttcctactt cgacttgttc ggtaaagtct
    30121 gcaggcagat gtaagataat gtttacacga ttaaaataaa gagcatcatc gatttctttt
    30181 tgtgaggttc catccaatgt aacgactgtt tccttgtcat tcaaatattt acgaaggcca
    30241 tcgactacag gcgactcttc ttcactaatc aaggcgattt tcgcatcttg cagagaggta
    30301 ttttcaacat caatattttg cgcaaaaaag aatgtggcaa caacagtgat aattacacct
    30361 atcagtaaat tcgatttatt tgctttaagc aatgtccaaa tcgttttata aagcgtcata
    30421 acgcacccct ctttccatcc aagcatttcc taacgcaaaa aggaaaataa atccgatcaa
    30481 ccatagaaga ttggtataaa aactagccaa atcagtgtag aaataaagtt tatacaaact
    30541 ttcgctgatc aagttgacga gatttaattg accaacaatc ggaatatgaa gatcaatcca
    30601 atacttcact tgttccgacc ccatcatacc agcaaaaaag ctcatcgcaa tcgtcactga
    30661 aatcaagatg ctatcctttt gcaccagatt catttttggt atcaaattac caaccaatgt
    30721 acctaacaaa atcgcacaca acgaccccag agcggtaaca atcatcaacc attgccaacg
    30781 ttcaccaaag tctacttgat agataaaacg aaagacagct aacataatat aaatctctgt
    30841 caaaacaatc gtaaagcttg ctgccaaatt agcgattgaa actaacgatt gctttctagg
    30901 aatcaaggac aagcgaatac catttgacga ttgatttgct tgctgatcct gtacattgcg
    30961 gaccccccac atcgtcccgt agagaatcgc cattccaatc agagtgaaga aataaaaact
    31021 tttgaaactc atattacgac ctgcatgaac ttcttgaaaa gatacttctt gttcaaaaac
    31081 agtcatgatg ggcgcgagat caacgttctc cacttctgcc actttactcg aaattatacc
    31141 aatacgctgt aaaaattgat ccatgacgtt tttcatcaac gtctgactca ttcctgcttg
    31201 acccacatgg agcgaaacag atgaaccatc aatgagataa aaaccagcca aatcatctgc
    31261 atttagcttt tcattcgctt ctacttccga tagttcctgt acttcaaata atggtttatc
    31321 attgacttcg acttgttcta agactgtggt aaaattttcc gaaagcgctg tttgatccgc
    31381 tttgattaca attccagtat taatcgtttc gagattgcca ccttgatcaa tctcgccaaa
    31441 ggccgccata aacatcaatc ctaaaagaat tggaaaggcc aatgtccaaa acaacatgga
    31501 tttatttcgc agcagtattt ttgtgcgata tttataaaaa tgccaaaaca ttatttcccc
    31561 tcctctagtc tctcaaatcc ttgccagtaa tctctaaaaa gacatcattc aaggacgctt
    31621 ctttggttgc tatttttccg taactgatct tttcttcttg taagattgta agaatcggca
    31681 acaatactgt atttagttca gtagttgtta cttggagctg acctttttta taagcgattc
    31741 catcagaagc tattgttcgc tgacaacacg ttttctggga gtaatgggat gtcgatcgtc
    31801 aaacgatccg ccgtccgtac catatctttt agagattctt tggttccttt agcaatcacc
    31861 tgtccttgat ccatgatcac gatctggtcg caaagaattt ctacttcttc catgtaatgt
    31921 gttgtataga caatggttgc tccttgtcga ttcagctcct tgatactttc taaaatttta
    31981 ttccgacttt gggggtctac agctactgtc ggctcatcta aaaaaattaa ccgcggcttg
    32041 tgggcaatcc cacatgcgat gttcaaccga cgtttcaagc caccactaag ttctcctgga
    32101 cgaaacgaaa catatttttc taatcccact aattgaatca cctcttcaat caaggctttc
    32161 cttttctctt tttcggaaac atataaaccg caaaaataat cgatattttc ttgcacactt
    32221 agttcgtcaa agaccgcaac gtcttgagga acgaccccaa tcattgcctt gacagcataa
    32281 ttttcaggag tcattgttgt cccgaacaat ttaatggttc ctttgtcata atttaacaaa
    32341 gaaagcatac agttgatggc agtcgatttc ccactgccgt ttggtcctaa cagacctaag
    32401 acttctcctt cttgtacttg cagatcaaaa tgattcaatg ctacaaaatc tccatagcgt
    32461 ttgactaatt tatcaatttc gataatcatc atgaaaaact ccttccaaat ttcttgttga
    32521 tctgactata ataaagtgca agaaaagaaa ctagtgacga ttggctaaac cacacatgac
    32581 atttgtcaca atgaaggagt ctgctatgca taaatctcgg ctactcaacg aaactagctt
    32641 tctactggtt atctcattgc ttttgctggt aagtcaagga ttcgttgcta tgcaagttat
    32701 ctatttattg atcgccgttt tattcactgg tgtcagttat ttactgccta caaagccaaa
    32761 ttggattttc caagttattc tatttctttt atgtgccttt ttacctgatc ttgtcctgtt
    32821 tctgccagcg acgatgcgag tgatcgcccc tgaagaaagc caccttgatt acttgttttt
    32881 gttaggtttt ttgttgattt cgacggttgg taatcatccc cccatcaagg tcgcctttgt
    32941 cgtaattttg ctgttttttt ccttttactt acgaaaaaaa gatcaacgaa cgaccgatct
    33001 tgagaaaaag tacattgtct ccaaagatac tcattgggaa aaacaacaag agttatcgga
    33061 acaaaataaa gcattggccc tgtcacaaga aacacaatta gccttgcaaa ttgtggaaga
    33121 acggaaccga attgcccgag acatccatga caatgtcggt catctgctgt caagtgcgat
    33181 tttacaaatt ggtgcgcttg aaatgatcaa tcaagaacca aaactaaaag aaccattaga
    33241 aaatttaagt gccaccgttc acactgggat ggatcggatt cgtcaaagtg tccatgacct
    33301 ccatgagaca tcgatctctt ttcaacactc cttagagttt ctgatcgctg attttccttc
    33361 ttctgttacg attgaaggaa acttgtttga aggattgaat gaaacgcagc aaaactgctt
    33421 cttaatggtc atcaaagaag ccttaaccaa cagtatcaaa catagtcatg ccaaaaccgt
    33481 tgttttacat tttcgaacgt tgccggcctt ttatcgcttg caaatcacga atgatggcac
    33541 cagcccacaa aaaaattctg ctcccggtat tggtttgaaa acgatgcgtc aacgtgttca
    33601 agaacaaaag ggacaagtcc atattttcca agaaaaccag caatttcagt taacgatcat
    33661 tttaccaagg gagggacacc aatgataaca gtagtcatta ttgagatgat ccatttgtga
    33721 cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat ccgtgtgtta ggcattggtc
    33781 attgtgccaa ggatgcgcta gtcttatacg aaacccatcg tcccgatgtt ttgctgacgg
    33841 acatccgcat gcctaaacaa acgggcatcg atgctgccaa agaaattttg gctaaatttc
    33901 ccaaagccat ccttttgctt ttgacgacat tcaaagatga agaatacatc cgcgaagcat
    33961 tttccatcgg tgtcaaaggg tatttaatca aacagaactt gcaagcgatt attccttctg
    34021 tcaaatccgc ttataatgga caggccgtat ttggtaatga aatcatcgag actttcactc
    34081 aaatgatgaa gaataaaccg acgattgata gtcatgctta tgcttctttt agtgaacgtg
    34141 agcttgcaat catcaaagaa gtagctgccg ggaaaaacaa taaagaaatt gcagatgcct
    34201 tgtacttgag tgacggcact gttcgcaatt acatcagcca attactagaa aagctagatt
    34261 tacgggatcg cacgcaattg gctatctatt tttatcagca tttgcaataa gtcttatcta
    34321 acacaaaaaa acctgaatga tgctccttcg catcattcag gtttttttca catcttctat
    34381 tcaattgtcc cgcaaaccat ttcctcacga ttgaaagaat caaggattcg cagggctgcc
    34441 aggattcgtt ggattgttcg gcgtgttagg cggaaaaata ggcgccttgt ctaacgtttt
    34501 ttctagtttt gattcttcac gatcgtctcg ttctacatca ggactttcat tcaatttgaa
    34561 ttctttttcc atctcatcta actttttaaa gtcattgcca gattcttttg ctaatttcgc
    34621 taatttatct tcggtttctt tagccataaa gtaacgcccc ccttttgatt tcagcttagc
    34681 atatcacact gaaaaaacaa aagaaaacgc taaccaacga tcatttttag ctatttctca
    34741 tctttaaaat caagcatttt ttgccggtat aaaatcgtag ggagaactgt tttccatacc
    34801 aatcatcgga tctactaact ggttatcgat cacttctgct gtaagaacga aatctttaga
    34861 aacagcaggt tctacctctt ctttcttttc ttctccaaca aatgaagaaa acagatctgg
    34921 ttccactgtt tccattactt ggtttttcgt ttcattcgtc ccactgtcat cttctacagg
    34981 tagcttttct tttacaaccc gtggctcttt tgtatagttc ccatcttcaa atggatcatt
    35041 tccgaccaaa gaatcttcat aagctgctag tttggtccgg agagtgatat tcattttatc
    35101 attatccatg atccttgatt tcaacgtagt catccggctt gctgattcat tcgctacaca
    35161 agtgacatag gcttgctctt cccctaacaa aatcaacagt tccttactgc gagtcactgc
    35221 cgtgtagagc aaattacgct gaagcatccg ctgatattga tggaccatgg gcaaaataac
    35281 catcttgaat tcactgcctt gtgatttatg gattgagcaa caataagaaa gagtgatttt
    35341 attccattcg tttcttttat aagtgacctc atttgcatca aactggataa ctaattcatc
    35401 cactttatct tcagaatctt ttgccaagat gatacccgtg atttctccca tatctccatt
    35461 gaatacattc aattcgggag tattgactaa ttggaggact ttgtctccta tccgataaac
    35521 ggtgtcgttc caatgtactt ccttttttcc aggtttccct ggattgaata tctcctgcat
    35581 cattttattt aacgcatcga tccctgcagg tccccgatac attggtgcta atacttggat
    35641 atcttgtgca cggaatccct tatttttcgc tttttccaca attttagaga tcaagggttc
    35701 aatttgataa gcatcacttc ggaaatagga acgatctttt tgattctttg tgaaatcttt
    35761 tggcagacgt ccttctttga tctcatgggc aagaggaata atactagaac catcaccttg
    35821 ccgataaatc tgattgagtt ccattttggg aatttgttcg atttccaaaa gatcatggag
    35881 tacttgacca ggaccgacag agggaagctg atctttgtct cctactaaga taacttgcat
    35941 attcgtcgga atcgctttga ataatgtgtt agccagccat gtatcgacca tagacatctc
    36001 atcaacaatc aaaagtccgc cttctaattc tttcgtactg actgaaggtg ttttttcccg
    36061 tccattcagc cccagaagac gatggatcgt actggcaggc agacctgtcg tttcgttcat
    36121 tcgtttagct gctcggccag ttggtgctgc aagtagaata ggaaaaattt cttgggtata
    36181 ttttgaagga tccaattcca gtccgttcaa ctctgcaaaa agattgacga ttccgttgat
    36241 cacagtagtt ttccccgtac ctggtcctcc tgttaaaata aatagagggg aacggattgc
    36301 ttcagcgata gcttgttctt gcgaaggtcc atactgtatg ccgaatattt tttcgatacg
    36361 ccgaatattc ttctgcactt ctttttcagg ataatggatt tcttttttac gactgagaag
    36421 gcgctggatc gaattaccga tcccccattc agaaaagtac aaactgtttt cgtaaagatt
    36481 agtctcttct tgttgtatct ttccttcttc aacaagttgg ataattacag tagcaacctg
    36541 atcaggctgg atttcaattg ggcgacttgt ttctaaaata cggatcgttt gctgaagcaa
    36601 tgtctccgcc ggtacgtatg tatctcctgt ttgaactgca tgctgaaaaa tctgatgcaa
    36661 gatagctgct cgaattcttc tcggtgaatc tgctgcaata ccgatttgtt cggctagatt
    36721 atctgcacgt ttgaacccga ttccttctat gtcttcgact agctggtacg gattttcttc
    36781 gatgatatcc aaagactcgt tcttataggc ctggtagata ctgaaagcta actgactacc
    36841 gaacccgtaa cgattcagtc ccatgatcac ttgatccatc ccgtggttta gccgaatcgt
    36901 ctcagcaagc atttctcttt ttggcttcgt caaaccggag atttgtttta ataccgaagg
    36961 atcagcaatg attttttcga tagcatcttc tcccagcaat tctacaattt tttctgctgt
    37021 tttcttcccg attcctggga atttttcgct ggaaagatac gctacgatcc catttgcagt
    37081 tgttggcttt tcttgctcat atctttcgag tttaagctgc cttccgtatt taggatgatc
    37141 cactaattct ccataaaaac gataagtctc attttcttga atatcgccga aacttccggt
    37201 gactacaatc tctttttcac ggtaatcagc tgtggtttca ctgatgtgta ccaaaagcac
    37261 tttgtaaaaa ttactaggat tttgaaaaaa aatcgcggca actgtaccaa cgaaataagg
    37321 catttcttct ggctgcatcg gaagccgcct ccttcttcta tagtaaaggt tctggtgctg
    37381 tttcagttcc taaaaaatct gtttggttcc atactttcag ttcatatggc aataatctgc
    37441 aaggctcttc agtctctaaa atcgtcaaac tactattgaa caatccaccc atttcacgca
    37501 actcacttag ctcttttcct gtcatccatt gaatggctgc agttaaagaa gctccatgtc
    37561 ctacgaataa aagtgggcca tctttatgtt gggcaaccgc atctgctaca acagtttcga
    37621 tacgtgtgat tgccgattca atcgtttctc cgtgaaaagg agaaggatca tacagatcta
    37681 aatgattacg caaattcggc agattatccg gatatttctg gtacatttca tcgatcgact
    37741 gaccttctaa agtacccagc cctagttctc ttagcttatc tgtataaaaa atctctaccg
    37801 gacgagcaag ctctttgttg ataccttctg ctgtatcacg tgctcgcttt gctgtactgg
    37861 aataaattgc tttaaaagga acatatttta cctttttccc aaatgctttg atttcttcgt
    37921 aactttgtgg caaaagcgga gaatcgccat tgctcccttg aaaacgtctt tcctgattcc
    37981 attgtgtctt cccatgtcgg gtaaaatata gttccatacg cagcaaactc ctgtctttct
    38041 tttattttac tctataataa actgtctaat ccagcttgtc attttcatag tgatgcgtca
    38101 attctaaatt agggaatcct tgctggcgaa gtgcttcata cactatcatt gcagctgtat
    38161 tcgataaatt caatgagcgg acatgctcat cattcatagg gatgcgtaaa cacttttctt
    38221 cgttttcacg cataaatgtt tctggcaaac cggttgtttc ttttccaaat aaaaagaagt
    38281 gctcttctcc atcagcaaaa ttttcgtcac tatatacttt atgggcaaat tttgagatca
    38341 aatgtagttt acgattacct aaaaatgtta aaaaagcttc taaattactg tggtaagtaa
    38401 tatttacatc attccaataa tccaaccctg cccgttttag atgtttgtca tcagttgaaa
    38461 aacctagtgg ctcgattaaa tgaagcggag tattcgtggc tgcacacgta cgggcaatat
    38521 ttccagtgtt tgcagggatt aatggttcaa ataaaacgat atgattcatc aggatcattc
    38581 ctctcatatt tatgttcgtt tctattttct gacaatttga tttttgcata aaaaaaaacg
    38641 tatcaactcg gtgtcagtca ctgcgagtcg ttacgttagt gaagctcttc tcactaactt
    38701 ttatcagaac aggaacctga taaaaaccaa tgaaaaaaca acttgttgct aatataacac
    38761 cctcaacttt tttttgcaac ctctactcat taaaaactgc tagaaaaacg ttttctctta
    38821 gttcctttta ataattctat gtcaattgta atcttcttca aaggattctt atataagtct
    38881 gctttgattc gctcttctac ctgccattct aaaggtgaca tggccaaaag atctttttga
    38941 ttttcctctg gtatctcaaa ttcataggtt acacgctgac gttcagttaa tttcattgct
    39001 tcagcaaact tggctactac acgttcattg gaataactct gtttttcagg ttgattcgga
    39061 taaaaagcct gacgtagttc ctttaaataa ttttcttgtg gcaccacctt caacacttct
    39121 ccatcttctg agagtacacg ttggaattct tggtaatgag aaggcgaaaa gatatttaaa
    39181 accgcgttga atgtttgatc agcaaacggc aaattggtca aatcagctac acaccaaaat
    39241 gcttcggcag attgttctgt agctgcgtag actccttctt ttgaaatatc aaatccaaca
    39301 gcattttgaa tctttttcaa ttttgaaagt tcattcaaga agctaccttc tccgcagcca
    39361 acatctaata gcgagacttc ttcaggtaaa tgctgcgcaa tagtctcaat aacgggctga
    39421 tacatgccag attggatcat tctacgcctt ggaacgaaca ttccttgatc gtattctgtt
    39481 ttaaccgcat gggataaaaa ataaagtgtt ccttttttgg aaaggtcaaa acggtgatta
    39541 tttttgcata tcaagccatt atcagacttt tttacttctt cttggcaaac cggacagcgg
    39601 aacagctgat gattttctgc caaaaactgg cgtcctcggt caattttttt taacatagtt
    39661 ccccctcatt tctgttttat cttaccgtta gatgctcact ttggcaactg ctaaaaatat
    39721 gatacaatgc ctagaagaaa gtgaaagata aaggagaaga ttagatgatc aaagaatttt
    39781 gcgctgaaaa ctacacatct attcctctag caattgctag aggagccgat cggatcgaat
    39841 tgtgtgacaa cttagcggtc ggtggcacaa ccccgagtac tggtgtgatc gaagaaacgt
    39901 taagttatgc tggggaaaaa gatatccccg taatgacgat tattcgtcct agagggggcg
    39961 acttcgtcta taacgatatc gaattgaaaa tcatgcatac tgatttgatt gaagcaaaga
    40021 aattagggac ggatggcgta gtatttggtt gtcttactcc ttcaggctgg ctagacgaag
    40081 aggccttaga actattgatc gaaacagcag aagggttgca gatcaccttc catatggctt
    40141 ttgacagtat tcctgaagat cggcaattcg aagcaatcga ttggctggta gaacatggtg
    40201 ttgaccgtat cttgactcat ggcggtcctg ctgacaaaac tattgtagaa aatttcccac
    40261 ggcttaaaga attgattgcc tatacagatg gccgcttaat cattttgcct ggtggtggtg
    40321 tcactgcaga gaatgcagca catgtagcta aagaattgaa cgtaagcgaa gtacacggaa
    40381 ccaaaatcgt ggatcttcag ttataatctt ataaacaaaa aaacatttgc aacaggcata
    40441 atgcttattg caaatgtttt tgttttttta cataaaatag cccataaata aagaagcaat
    40501 attgaaataa atcagtacac tcgtcaaatc gctcaaagtc gtgataaagg gaccgcttgc
    40561 tactgctgga tcaaatccta gacgatccat tagcatcggg attaaactac ctgcaagatt
    40621 cgccactgtg attgcaaata gcatagccat ccctatgaca aaccccaaga taaaattatg
    40681 tttccaaaaa cctacgatta gaaaaatcgt gatccccgta acagctcctg tgacaacgcc
    40741 ggtcaagact tcagccaaaa caagtcgaac aaatccatga tctttctcat cagaaatcgc
    40801 aagtcgacga acagccacag ctagcgactg ggttccagca ttaccggcag ttcctgtaat
    40861 cagcgaaatg aagactgcta aaatactcgc ttcacttacc aagtcttcat agtgactgat
    40921 taaagaagcg gtcgacatac ctaaaaacaa cagagtgatc agccaaggca aacgtcgaga
    40981 cgctgccttt actggatttt cattaatttc ttccacattg accccggcaa gtcctgaata
    41041 atcgctagca gcttcatcat caataacgtc gatgatatca tcgactgtga cgattcctaa
    41101 caaatgatca tcataatcgg taacaggaag agctaagaag tcataatctc taaatgtttg
    41161 tgccacatct tcttggtcgt cgcctacatg aactgaaagc actcgttcgc tcatcagatc
    41221 agaaatcatt gcgtcgtcat cattcacgat taggtcgcgc agagaaataa cccctactag
    41281 ctgaccttct tgattaacga catagatgta atagattgtt tcagctacat ctgcttcgtt
    41341 cttcagcacg tacatcgcag agcgaacagt ttgattggct acgatcgaaa caaattcagt
    41401 tgtcatgatc gcgccagccg tttcatcctc gtaatggaga agttccttga tttcactggc
    41461 atcgtctgcc gacatcaaac ttaaatattt agcaatttgc tttttatcca gcgtattaag
    41521 caagtcaaca gcattatctg tatacatctc agaaagcata tcagctgcat agctaggacg
    41581 catttcatct aggtattctt tcatatgttc gttgtcttct tcaatcacat cgaacatatc
    41641 cgctaattct ttaggcgaca gataggaata gatcagctgc ctgttctctt ctgtaagaga
    41701 ttggtagaat tgcccctgct catagatatg catctctaaa aaactgtttc tgaatgcctg
    41761 aatattattt tcccttaaat cttgagtcaa ctgggcaaat ctttcctcta attcttgatt
    41821 ttcatccaaa ccaatctcac ctcgccttat tcttcaaacc gtctcacaac tgcctccatg
    41881 tctgcggcta aaggctgctt gaactccagc aactcttctg taaatggatg gtaaaaacgc
    41941 agatcataac aatgaagggc ttgtcttttt attcccagat ccattcgccc gccatacata
    42001 tcatcaccga gcagggcaca gccaattgcg gaaaaatgaa ctcttatttg atgtgtcctt
    42061 cctgtatgca gctggatatc aactaacgcg atttctgtta gtcgcttctc tatccagtac
    42121 tcagtctgtg ccgaacgtcc ttcatcagag accattcgct tcaatagcga agaataatct
    42181 cgggcgatcg gcaaatcgat atgtccatgt gccgataaat ctgaaagcct tccactgact
    42241 aaggcctgat attttttcac aaacttcttt tcacgaagtt gcatatctaa ttttgcgtgc
    42301 gcaaatccat gctttgcaaa aagcatcaaa ccagatgtat cccgatctag tcgcgtcacc
    42361 acatggatta cttggttttc gtaaccttgt tttttataat aagcctttac acgattagcc
    42421 attgtcccat ttggatggta ttgggcagga atagacgaaa cgcctgctgg tttatttact
    42481 accagaacgt gttcatcttc atacacgata tcgatcggtg tttcatccaa aagtacggtt
    42541 tcatgttctc cttcagcagg aatcacaatg gttaccttat catccttagc tagagaaaac
    42601 aagacatttt ctacttgatc attaactttg atttgaccgc cttgaaattt tattttcgct
    42661 aacagaccct ttgagatccc tttttctttt aaaaaatatt ttacttgctg agaatgatct
    42721 ttctcataat cccatgtaaa ctgcatatca atcctcacta ataaaggcat ctttcacacg
    42781 atgccaaaaa tgcatatgtc gataagaagc aaaatggatc cgttcttctg ctattcgata
    42841 ataaattgag cggatatttg cttttgcgac attcaattga tcaaccgtta caagataatc
    42901 ttctgtattt tccaatttta tttctagcca ttcatcatgg gcaatcacga tcggagaacc
    42961 caaagtacga aacactcgat tattcagtga agcaatctct gccaactgaa aggcattgat
    43021 actaggatgg atcacagcac cgccgacact tttattatat gctgtcgaac cagtaggcgt
    43081 agaaatcgtc aatccatctc ctctaaaact ttcaaacagc tcgtttttga tgtatacatc
    43141 ggctaccatc gtacgattgg cccgtttaat cgttgattca ttcagtgcta aaaaatgctg
    43201 gtccggtgtt tcatccaaat aactgatccg aacatccagc aagggataac taacactttg
    43261 ttcccggttc gttcgtaagc tgtctactag ttcttccagc tcgtaatccc gccaatccgt
    43321 gtaaaaccct aaatgacctg tatgcacgcc caagaaacgc acatcattca gacaatgact
    43381 gtagtgatgg aacgccgaca ataatgtgcc gtctccgccg actgagatga ccaattctgg
    43441 ctcatgatca tctcgttgaa tccctgcttg ttctaaaagt gcgttcaaac gttctgtcac
    43501 ttctatagtc tttgcttctt tattatgaac gatcgctact ttcatcccct gcctcctctc
    43561 tttcgctttc tttcttcaaa agcgttcgat agtagacatc atctgatttt cctcttccat
    43621 gggagaataa ccgctgtgct tcttggattt cttcacggat actggacatc tcttcatcca
    43681 actgataggc agcttctgcc gcacgctgaa gtcgttcact catttcttct ggaaaaacgc
    43741 cttgatattt ataattcagc gaatgctcaa tcgttgccca gaaattcatc gcaagagtcc
    43801 gaatctggat ttccgccaaa attttcttct caccgctgat cagctgtaca ggatactcga
    43861 tcaccagatg ataagaacgg tagccacttg cttttttatt tgtgatgtaa tctcgttctt
    43921 ggagaacttt catgtctttt cttttacgga tgatctcaac aacttgatgg atatcttcta
    43981 caaactgaca catgatcctt aacccggcaa tatcagacat ttcttcttct aaacgtccca
    44041 ttggaatatg ccgtaattgt gattttgtta caatactatc gactggcttg actctcccag
    44101 tgacaaattc aatcgggaca tgtcgatttt gttcacggaa ctgtttgcgg atcccccgca
    44161 gtttcacttt caattctgat acagtttgtt catatggtgc taaaaatgct tcccaatctc
    44221 gttccatagc tgatacctct acttcattaa cattcattgt ttattctacc atatatgcaa
    44281 agttttttcg tactcttgat ttatgcattg accaaaaaaa gaaaattagg caaaatagac
    44341 aaaaaggagt cgatcaaatg acacaaatgc ttgaaatcga atataaatct atgctgacaa
    44401 agcaagagta cgagatattc atcgctcact atcaattaac agataaagat ttccgtgtac
    44461 agactaatat ttatttcgat accgttgatg aacagctgaa gaaacaaaat tgtggcctac
    44521 ggatccgttt cgttggtcat caagctgaat atacattaaa aacacctgct gaaaaagggc
    44581 gtctcgaaac aacggatacc tttataacag aagaagcaga agcatttatc ttcgaaaaac
    44641 gtctgcctcg ttcaggcgca gtttttcaaa aactttctga tttgaatatc gatcctgctt
    44701 cattaatcca aaccggaaag cttacaacaa aacgggctga attccctatt gaagaaggac
    44761 ttttagctat tgatgaaagc tggggagaca atctacacga ttttgaactg gaactagagg
    44821 taggggatgc atctgtggga aaaattgctt ttataaattt tttaaaacgt ttttctttac
    44881 cttatcgtcc tgctaagaac aagatccaac gtatgcttga agctaaaaat tagaaacctt
    44941 tcattttcac aaggcacttg agttcatttt tttacttttt tctgttaagt tataggatgt
    45001 gaaagaatct agtcactagt tgtatagaat ctattttggg ggagattcat atgatcgaaa
    45061 tctatttatt tgttaatcct ttaggggaat actgtttcga aatggaacaa cagttattac
    45121 aatttatcga agaagagtac gggacaccat caaaagaaaa gatgcaattt cgctttcttc
    45181 ctttggtcaa tttgcagaca attggggatg tcatgcaaca aaaaggaatt tctcagaaca
    45241 atcttgagga acgtaatcac ttattctcaa tgacttattc tgcagcattg gactgcaaag
    45301 cggcgcagtt tcaaggaaag aaaaaaggcc gccgtttttt gatcaaattg caagaagcag
    45361 tcggctgtaa tgacatacca tattctcaag aacttgctga atccatattt gaagaaatcg
    45421 gcggcgattt gaatatgttt aaagaagatc gtcagtctga cttcgttaaa gaagtattct
    45481 tttctgatca aaaaattgct cgggaaatgg gcatcacaaa acacccttct gcagtcgttt
    45541 acaattatgc ctgcgaccgt gattttggtg ttttagtaga agtaaaaggt acaagtagcc
    45601 tttggagaat cacagagctg tgtaaaacaa cagataactc atatcaagtt tttaatggtg
    45661 aacatttaca tacaaatgat tctcagttcc attcaagaac tagccattta cacttgcttt
    45721 ctaattaaaa aaatacgtcg actcccttac aagagagccg acgtattttt ttcgtttcta
    45781 ttcgagaaaa tcaaaaatag aaagccttat aaacgatcga tcaattcttc taattcattt
    45841 agacgttgtt caaacatcga cattgcatct tcaatatatg cagcctgtgt catatcaact
    45901 cctgcttttt tcattacttc cacaggataa tcgctgttac ctgcttttag gtaagccaag
    45961 tagttctcta gtgcctctgg ttcctggttt agtattttct tcgccagtgc tgaagcagca
    46021 gaaaagccag tcgagtattg gaaaacatag taattataat aaaaatgcgg tatccgtgac
    46081 cattcgaatt taatttctgg gtcttcttcg actgctggac cataatattt tgcattcaat
    46141 ttaccataac tatcacttag gtattcacta gtcaatggca cacctttttc atcttccgta
    46201 tgcatgaaat gctcgaattc agcgaattgt gtctggcgga aaactgttcc tttaaaccca
    46261 tccaagtaat ggttgagtac ataagcccgt acacgagggt ctttttctgt ttccaataaa
    46321 tactccgtta ggatattttc attcgttgtc gaagcaattt ctgctaaaaa gatggagtag
    46381 tcgccgtaca cataaggctg gttcgaacga gtgaaataac tatgaacact atgtcccatt
    46441 tcgtggacaa gcgtaaatag ctgatccagc gtatcatgcc aattcaataa aatatatgga
    46501 tttgtgtcat agcttcccga agaataagca tcgcttcgtt tccctttatt ttcgacaaca
    46561 tcgatccaac gttcagagaa tgctttttct acgatggcca tgtattcttc acccattggt
    46621 ttcagtgctt ctaaagcttt ttcttttgct tcttcgtacg taaaggtaat tggagcttcc
    46681 cctaagaccg gtgtataaag atcatacatg tgcagttttt ccacttctaa taaacgtttc
    46741 cgtaattcca tgtatcgatg taacaaaggc aaatgcttgt ttaccacgtc taccaaagta
    46801 tcgtatacac tttcaggaat atgattattg ctcaaagacg cttctctggc agagctgtaa
    46861 ttacggactt tcgctttaaa attatgtcct tttatatgtg tgcctaaagt agaagcaaat
    46921 gtatttctaa attgttcgta aacactgtac aatcccttaa atgctgcttc gcgcaccctt
    46981 cgatccgtgc tttctagcaa ctgaccatac acgccatgag ataattggac tatttcacca
    47041 ttttctcctt caatcgttgg aaaaactaga tctgcattat tcaaaacagc aaatgtatca
    47101 cttgaagcat caaagatttc acctgctcca gcaagaagag attcctgctc agcagataag
    47161 acatgggctc gattatctac tatttgctgg atataatggc gatagacttc caattttggt
    47221 tcttctttga aatattgcca aatctggtca tctgacaatt gcaatatttc tggttcaaac
    47281 caggaaacgg cttcactaac tttcgaaaac agactgcttg ctcttgcata aagcgcttgg
    47341 taatctgtat ttccagtgtc ttgatcgttt ttaaggtgcg catatacata aatgacttca
    47401 gtttggcgat aaaccctcaa tacgaattca atcgcattta aaaattgaga agcgccttga
    47461 tcaagtgttc ctttgtgttt ttcagattgt tttaactctt ctgataattc caaatatttc
    47521 tcatcaaact cttggtcgct tgaaaagatc ttggtcaagt cccaagttaa attttcaggc
    47581 aattcttctc gttttggcaa ctgctttact tccatgaaat ccactccttt tcttccctta
    47641 gctctatcta tcaaaataga tcatagtcta acatccacat gtcacagcat ctaacttttc
    47701 tactcattca tgtgtctgct tccatcaatt ttagcataac taaatcttgt tttacataga
    47761 aaaccgattc ctagcctatt tttatactta ctattttttc aaagaagaca agtgtatgca
    47821 ttcgttaaaa aactgctgat agaccatctc ttcatcaatc ataggaaaaa gccattccct
    47881 tttatgttct tttatttggc aaagccatga gttgaaatca ggaactttct ggttttccaa
    47941 ggcttcttcg tacaacattc gataaagaaa aacctgttct tgtaaataaa agaaaaaccg
    48001 acttttttga tagatccacg atgatagatg caaaagatgt ttatgacgca agtagcattg
    48061 ttcttgtatg gatactgtat tcttttgctt gtgaataagt tggcgtgtca gccatttctt
    48121 cccatcttca ctcacttttt ctattttttt catttggttc attttaattt cgttactagt
    48181 gaatatttct ttaaggtccc tactagaaaa agtccagcag ctactgcaaa aggaaatatg
    48241 ccctgataca gtctctgtaa tatgatgata caaataaagc ttttgtttct tgagatcagc
    48301 tttccagcaa tgcactcctc tttttctatt atagtagcag caactttttt ctatcaatga
    48361 gaattgcgat tgtccaagaa aagaattacc aagtatccac catacttggt aactgttatt
    48421 caaataattt tgggttcttt caataaatct ctggtgagac agtctagaac attggatttc
    48481 tattgctaga ttcgccaaca aaatatccgg cctttgccgc aacctcggca aataggcttc
    48541 caaaaatact ggttctactg actgtcccag ccaatccatg aagcattcct ttaactgtaa
    48601 gtgctctttt gattctcttt cactaaagac cgaacagtta tctgattttt gatgggcaaa
    48661 atgagcacat tttttctttc cctttttcaa aatcactctt tttcgacagc cacgacaata
    48721 atatttcttt cctttttctg catgtttagc aaaacagcac ttattatatt cattcattgc
    48781 aagcagcatt tttctccctc tgaacttatt tacgtctaag aatttattta cacgaaaaaa
    48841 gagggcgtga aaaagctatt ctgcatcaag taataagaac agctaaacaa aaatagttcc
    48901 caatattttt cgtttggttg ggcttgttac cgcagatgca agcttttgaa cacatttata
    48961 cgaaaaaaag actgcgacac aatttttgtc acagtctctt actataatgc tgatttatac
    49021 aaaataaaat ttcgttcgct cgatagcatt acgcgccatc agacattgtc catattcatt
    49081 caatgtttct ggtgtacgat cgctcttttc agcaaactcc aaaattcgag caatctgatt
    49141 ctctacatcc gcttggttga gattttccat ccagaagtga acgactaggt aatagcgctc
    49201 ttcgaataaa tacaaatcag tccaagcaga ctctagttcg acatctgttg caagttggat
    49261 cacttgttca aaatcttcaa aagcaaaagt tgcttgtctt tcctgttctt ctagggcgct
    49321 tccagataat tcttcctccg gatcgctgcc aataatctgc tgacgaagaa agtcactcac
    49381 atcttctgaa tcaacagtag aatcttctga ttgagaaatt tcttcatccg gaagattttt
    49441 actgataaac agttctaatc cgtctccttt tggcaaaact tggaaagtga cagcttcgct
    49501 tcctcggaac tcctcttcga tatcaacttc ttccaaaata ctataaaaga aattttcgat
    49561 ttctttgtga ttgccaagta aatccaaaaa tgaaacgccc cgttcctcta aatcttcatg
    49621 accaattagt acacggatcg tattttcatt aatatgttcc atttccattt tgctacacct
    49681 cactttgcca caatgtttaa tatagattca ttgtagcgaa atcagcttaa atgtaaagaa
    49741 aaaaaatctc gctgttaagc gaatttaata aaaccagcgg atttttcaac ctttcaagct
    49801 atcttattta actataacat ttttggcaga cgaatactat atagccgact tttcagctaa
    49861 ttttccctct ctatttttcg tgcaatcatc atcttctact acctttttat acatctaatc
    49921 aagaatactt tttgtaaaaa caaaaaaact tggaaaaact atctttgtaa tagtcttttg
    49981 gctctatact ttttgatgtt ggtgaacttc accaacatct ttttt
//
LOCUS       DACDUU010000012        49351 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.012, whole genome shotgun sequence.
ACCESSION   DACDUU010000012 DACDUU010000000
VERSION     DACDUU010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.012"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            316..624
                     /gene="rpsJ"
                     /locus_tag="IWL30_000718"
     CDS             316..624
                     /gene="rpsJ"
                     /locus_tag="IWL30_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAP9705506.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            655..1284
                     /gene="rplC"
                     /locus_tag="IWL30_000719"
     CDS             655..1284
                     /gene="rplC"
                     /locus_tag="IWL30_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAP9705507.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            1312..1935
                     /gene="rplD"
                     /locus_tag="IWL30_000720"
     CDS             1312..1935
                     /gene="rplD"
                     /locus_tag="IWL30_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAP9705508.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            1935..2225
                     /gene="rplW"
                     /locus_tag="IWL30_000721"
     CDS             1935..2225
                     /gene="rplW"
                     /locus_tag="IWL30_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAP9705509.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            2264..3097
                     /gene="rplB"
                     /locus_tag="IWL30_000722"
     CDS             2264..3097
                     /gene="rplB"
                     /locus_tag="IWL30_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAP9705510.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            3140..3418
                     /gene="rpsS"
                     /locus_tag="IWL30_000723"
     CDS             3140..3418
                     /gene="rpsS"
                     /locus_tag="IWL30_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAP9705511.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            3441..3788
                     /gene="rplV"
                     /locus_tag="IWL30_000724"
     CDS             3441..3788
                     /gene="rplV"
                     /locus_tag="IWL30_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAP9705512.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            3802..4458
                     /gene="rpsC"
                     /locus_tag="IWL30_000725"
     CDS             3802..4458
                     /gene="rpsC"
                     /locus_tag="IWL30_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAP9705513.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            4461..4895
                     /gene="rplP"
                     /locus_tag="IWL30_000726"
     CDS             4461..4895
                     /gene="rplP"
                     /locus_tag="IWL30_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAP9705514.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            4885..5073
                     /gene="rpmC"
                     /locus_tag="IWL30_000727"
     CDS             4885..5073
                     /gene="rpmC"
                     /locus_tag="IWL30_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAP9705515.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            5098..5364
                     /gene="rpsQ"
                     /locus_tag="IWL30_000728"
     CDS             5098..5364
                     /gene="rpsQ"
                     /locus_tag="IWL30_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAP9705516.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            5422..5790
                     /gene="rplN"
                     /locus_tag="IWL30_000729"
     CDS             5422..5790
                     /gene="rplN"
                     /locus_tag="IWL30_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAP9705517.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            5827..6135
                     /gene="rplX"
                     /locus_tag="IWL30_000730"
     CDS             5827..6135
                     /gene="rplX"
                     /locus_tag="IWL30_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAP9705518.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            6163..6702
                     /gene="rplE"
                     /locus_tag="IWL30_000731"
     CDS             6163..6702
                     /gene="rplE"
                     /locus_tag="IWL30_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAP9705519.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            6721..6906
                     /locus_tag="IWL30_000732"
     CDS             6721..6906
                     /locus_tag="IWL30_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAP9705520.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            6942..7340
                     /gene="rpsH"
                     /locus_tag="IWL30_000733"
     CDS             6942..7340
                     /gene="rpsH"
                     /locus_tag="IWL30_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAP9705521.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            7372..7908
                     /gene="rplF"
                     /locus_tag="IWL30_000734"
     CDS             7372..7908
                     /gene="rplF"
                     /locus_tag="IWL30_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAP9705522.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            8069..8425
                     /gene="rplR"
                     /locus_tag="IWL30_000735"
     CDS             8069..8425
                     /gene="rplR"
                     /locus_tag="IWL30_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAP9705523.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            8446..8946
                     /gene="rpsE"
                     /locus_tag="IWL30_000736"
     CDS             8446..8946
                     /gene="rpsE"
                     /locus_tag="IWL30_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAP9705524.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            8961..9140
                     /gene="rpmD"
                     /locus_tag="IWL30_000737"
     CDS             8961..9140
                     /gene="rpmD"
                     /locus_tag="IWL30_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAP9705525.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            9184..9624
                     /gene="rplO"
                     /locus_tag="IWL30_000738"
     CDS             9184..9624
                     /gene="rplO"
                     /locus_tag="IWL30_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAP9705526.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            9624..10919
                     /gene="secY"
                     /locus_tag="IWL30_000739"
     CDS             9624..10919
                     /gene="secY"
                     /locus_tag="IWL30_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAP9705527.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            10981..11628
                     /locus_tag="IWL30_000740"
     CDS             10981..11628
                     /locus_tag="IWL30_000740"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAP9705528.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            11819..12037
                     /gene="infA"
                     /locus_tag="IWL30_000741"
     CDS             11819..12037
                     /gene="infA"
                     /locus_tag="IWL30_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAP9705529.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            12070..12186
                     /gene="rpmJ"
                     /locus_tag="IWL30_000742"
     CDS             12070..12186
                     /gene="rpmJ"
                     /locus_tag="IWL30_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP9705530.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            12204..12569
                     /gene="rpsM"
                     /locus_tag="IWL30_000743"
     CDS             12204..12569
                     /gene="rpsM"
                     /locus_tag="IWL30_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAP9705531.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            12597..12986
                     /gene="rpsK"
                     /locus_tag="IWL30_000744"
     CDS             12597..12986
                     /gene="rpsK"
                     /locus_tag="IWL30_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAP9705532.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            13066..14004
                     /locus_tag="IWL30_000745"
     CDS             13066..14004
                     /locus_tag="IWL30_000745"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAP9705533.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            14032..14412
                     /gene="rplQ"
                     /locus_tag="IWL30_000746"
     CDS             14032..14412
                     /gene="rplQ"
                     /locus_tag="IWL30_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAP9705534.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            14684..15523
                     /locus_tag="IWL30_000747"
     CDS             14684..15523
                     /locus_tag="IWL30_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9705535.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            15499..16368
                     /locus_tag="IWL30_000748"
     CDS             15499..16368
                     /locus_tag="IWL30_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9705536.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            16358..17155
                     /locus_tag="IWL30_000749"
     CDS             16358..17155
                     /locus_tag="IWL30_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP9705537.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            17358..18104
                     /gene="truA"
                     /locus_tag="IWL30_000750"
     CDS             17358..18104
                     /gene="truA"
                     /locus_tag="IWL30_000750"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP9705538.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(18559..21228)
                     /locus_tag="IWL30_000751"
     CDS             complement(18559..21228)
                     /locus_tag="IWL30_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP9705539.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(21466..21654)
                     /locus_tag="IWL30_000752"
     CDS             complement(21466..21654)
                     /locus_tag="IWL30_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAP9705540.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            21924..22286
                     /locus_tag="IWL30_000753"
     CDS             21924..22286
                     /locus_tag="IWL30_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9705541.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            22338..24020
                     /locus_tag="IWL30_000754"
     CDS             22338..24020
                     /locus_tag="IWL30_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAP9705542.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            24137..26173
                     /gene="recG"
                     /locus_tag="IWL30_000755"
     CDS             24137..26173
                     /gene="recG"
                     /locus_tag="IWL30_000755"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAP9705543.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            26222..27223
                     /gene="plsX"
                     /locus_tag="IWL30_000756"
     CDS             26222..27223
                     /gene="plsX"
                     /locus_tag="IWL30_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAP9705544.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            27345..27587
                     /gene="acpP"
                     /locus_tag="IWL30_000757"
     CDS             27345..27587
                     /gene="acpP"
                     /locus_tag="IWL30_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP9705545.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            27855..28859
                     /locus_tag="IWL30_000758"
     CDS             27855..28859
                     /locus_tag="IWL30_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9705546.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            28860..29804
                     /locus_tag="IWL30_000759"
     CDS             28860..29804
                     /locus_tag="IWL30_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9705547.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            29804..30766
                     /locus_tag="IWL30_000760"
     CDS             29804..30766
                     /locus_tag="IWL30_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9705548.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            30793..31707
                     /locus_tag="IWL30_000761"
     CDS             30793..31707
                     /locus_tag="IWL30_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9705549.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            31733..33514
                     /locus_tag="IWL30_000762"
     CDS             31733..33514
                     /locus_tag="IWL30_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9705550.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            33862..34548
                     /locus_tag="IWL30_000763"
     CDS             33862..34548
                     /locus_tag="IWL30_000763"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP9705551.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            34568..38149
                     /gene="smc"
                     /locus_tag="IWL30_000764"
     CDS             34568..38149
                     /gene="smc"
                     /locus_tag="IWL30_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAP9705552.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            38158..38967
                     /locus_tag="IWL30_000765"
     CDS             38158..38967
                     /locus_tag="IWL30_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9705553.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            38978..39976
                     /gene="ftsY"
                     /locus_tag="IWL30_000766"
     CDS             38978..39976
                     /gene="ftsY"
                     /locus_tag="IWL30_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAP9705554.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            40163..42430
                     /gene="gshAB"
                     /locus_tag="IWL30_000767"
     CDS             40163..42430
                     /gene="gshAB"
                     /locus_tag="IWL30_000767"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAP9705555.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(42519..42971)
                     /locus_tag="IWL30_000768"
     CDS             complement(42519..42971)
                     /locus_tag="IWL30_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAP9705556.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            43121..44071
                     /locus_tag="IWL30_000769"
     CDS             43121..44071
                     /locus_tag="IWL30_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP9705557.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            44064..45023
                     /locus_tag="IWL30_000770"
     CDS             44064..45023
                     /locus_tag="IWL30_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP9705558.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            45020..45775
                     /locus_tag="IWL30_000771"
     CDS             45020..45775
                     /locus_tag="IWL30_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9705559.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHIGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            45798..46751
                     /locus_tag="IWL30_000772"
     CDS             45798..46751
                     /locus_tag="IWL30_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9705560.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(47395..47691)
                     /locus_tag="IWL30_000773"
     CDS             complement(47395..47691)
                     /locus_tag="IWL30_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAP9705561.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            48046..48402
                     /locus_tag="IWL30_000774"
     CDS             48046..48402
                     /locus_tag="IWL30_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9705562.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            48412..49311
                     /locus_tag="IWL30_000775"
     CDS             48412..49311
                     /locus_tag="IWL30_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAP9705563.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT        16379 a         8828 c        10658 g        13486 t
ORIGIN      
        1 attgcaggat caggaaaaaa gacaggagta ttataaatag aagaaacacg aaataaaaga
       61 gtcagtattt tctaaatgaa actttatgaa aacaaaatgt tttttacaag aaaaagcctt
      121 gctttgtgag tttatatata ttataatgac aaaggtgcgt ttactttagt gaattgcaca
      181 gacgtaatca gaatgatttc gtcggctaag aaacgagagg ttgcgacacg cccggacgct
      241 ttgccacgaa cgagcgtgac ggaaattttc gtggagctat gtctactttt caaaataggc
      301 gaaggaggga acaagatggc aaaacaaaaa attcgtatcc gtttaaaagc gtatgaacac
      361 cgtattttag atcaatcagc ggataaaatc gtggaaactg caaaaagaac tggagctagc
      421 gtgtctggtc caattccatt accaactgaa cgcagtcttt acacaatcat ccgtgcgact
      481 cataaataca aagattcacg cgaacaattc gaaatgcgta cacacaaacg tcttatcgac
      541 attgtgaacc caacaccaaa aactgttgat gctttgatga agctagactt accatctggt
      601 gtaaacatcg aaatcaaatt ataaaactaa aacaaaaata atggaggtgt actcatgacc
      661 aaaggaatct tagggaaaaa agtgggaatg acacaaatct tcactgaatc tggtgaatta
      721 attccagtaa ctgttgtaga agctactcca aacgtagtac tacaagtgaa aacaatggaa
      781 acggatggct acgaagctat tcaagttggt taccaagata tgcgtgaagt tttatcaaac
      841 aaacctgcga aaggtcatgt tgcaaaagca aacacggctc ctaagcgctt cattcgtgaa
      901 ttcaaaaatg ttgagctagg agaatatgaa gtaggaaaag aaattacagt agatgttttc
      961 caagcaggag acattattga tgtaacaggt actacgaaag gtaaaggatt ccaaggggtt
     1021 atcaaacgcc acggacaaag ccgcggacca atggctcacg gttctcgtta ccatcgtcgt
     1081 cctgggtcaa tgggtccagt tgcacctaac cgtgtattta aaaacaaacg tcttgctggc
     1141 cgtatgggtg gcaaccgcgt aactatccaa aacttggaag ttgtacgtgt cgatgcagaa
     1201 agaaatgtta tcttgatcaa aggtaatgtt cctggagcta aaaaatcttt aattactatc
     1261 aaatcagctg tgaaagctaa ataattgtag gaagaaagga ggaactaagg aatgccgaat
     1321 gtagcattat tcaaacaaga tggaagccaa aacggtgaaa tcacacttaa cgaagaaatc
     1381 ttcggaattg aacctaatga aagtgttgtt tacgatgcaa tcgtaatgca acgtgcgtca
     1441 ttaagacaag gaacacacgc ggttaaaaac cgtagcgctg ttcgcggcgg cggacgtaaa
     1501 ccatggcgtc aaaaaggaac aggtcgtgca cgtcaaggat ctatccgctc accacaatgg
     1561 cgtggaggtg gcgtagtctt tggaccaaca ccacgttctt acagctacaa acttcctaaa
     1621 aaagttcgtc gtttagcaat gaaatctgta ttgtcagaaa aagttgctga aaacaacttg
     1681 gtagcaatcg aaggcttgaa cttcgacgca ccaaaaacaa aagaattcaa acaagttctt
     1741 gctaacttat ctatcgactc taaagtattg gttgttctag aaaaaggtaa cgactttgct
     1801 gctttatcag cacgtaactt accaaacgtt tctgtagtaa cttctgataa tgtgagtgtg
     1861 ctagacgttg tatcaaatac aaaagtgtta gcaacacaaa ctgctcttac tcaaattgag
     1921 gaggtgcttg cataatgaac ttactagacg taatcaaacg cccagtgatc actgaaaaat
     1981 caatgcttgc catggatgac aagaaataca cttttgaagt ggacactcgc gcaaacaaaa
     2041 ctttagtaaa acaagcggta gaagctgctt ttgacgttaa agtcaaaaac gtgaacatcg
     2101 tgaacgtacg tgcgaaattc aaacgtatgg gcaaatatgc aggatataca aaaaaacgtc
     2161 gcaaagcgat cgttacattg actgaagatt caaaagaaat ccaattattc gaagctgctg
     2221 aataagcact aatagataac tcattaaatt aggagggaaa aacgtggcga ttaaaaagta
     2281 caaacctacc tcaaatggtc gtcgtaacat gacaggttct gattttgctg aaatcacaac
     2341 atcaacacct gaaaaatcat tgttacaacc attgaaaaac catgctggac gtaacaacaa
     2401 cggccgcatc actgtacgtc atcaaggtgg cggacacaaa cgccaatatc gcgtgatcga
     2461 cttcaaacgt aacaaagata acgttgttgc aactgttcaa acgatcgaat atgatccaaa
     2521 ccgttcagct aacatcgcgt tagtacacta tgaagatgga gtgaaagctt acatcttagc
     2581 acctaaagga ctagaagtgg gtatgaaact tgtttcaggt ccagaagcag atatcaaagt
     2641 cggaaatgct cttccattag aaaacattcc agttggtact gttatccaca acattgaaat
     2701 gaaacctggt aaaggtggac aattgatccg ttctgctggt acaagtgctc aagtacttgg
     2761 taaagaaggc aaatacgtat tgatccgctt gaactctggt gaagttcgta tgatcttagc
     2821 tgcatgccgt gctacaatcg gttctgtagg taacgaacaa cacgaattaa tcaacatcgg
     2881 taaagctgga cgctctcgtt ggatgcgtaa acgcccaact gtacgtggta gcgtaatgaa
     2941 ccctaatgat cacccacacg gtggtggtga aggtaaagct cctatcggac gtaaagcacc
     3001 agtttctcct tggggtcaac cagctcttgg ttacaaaact cgtaacaaaa aagctaaatc
     3061 agacaaactt attgttcgtc gtcgcaaaaa taaataatta ctttgaccat gtgtcgcaca
     3121 ttttgagagg aggttcacca tgggtcgtag tttaaagaaa ggaccttttg tcgatgagca
     3181 tttaatgaaa aaagttgaag ctcaagcagg ggttgaaaag aaaaaagtta tcaaaacttg
     3241 gtcacgccgt tcaacagttt tcccaagttt tgttggatat accatcgcag tttatgatgg
     3301 acgtaaacat gtaccagttt acatccaaga agacatggta ggacacaaat taggtgaatt
     3361 tgcacctaca agaacttatc gtggacatgc tgctgacgat aagaaaacga aacgctaatt
     3421 tgaggggagg ataaatccaa atggcagaac aaatcacatc agctaaagca actgcaaaaa
     3481 cagttcgcac ttcacctcga aaagcccgtt tagtaattga ccttatcaga ggtaaaagtg
     3541 tcgcagatgc aatttcaatc ttgaaattca caccaaacaa atctgctgga atcattgaaa
     3601 aagtattgat gtcagcagtt gcgaatgcag aaaataactt tgacttagat gttgaaaact
     3661 tggtagtaag cgaagctttt gttaacgaag gacctacaat gaaacgtttc cgtcctcgtg
     3721 caaaaggatc agcttctcca atcaacaaac gtacaagtca tatcacggta gtcgtatcag
     3781 aaaaataagg agggataatc agtgggtcaa aaagtacatc caattggaat gcgtgtaggc
     3841 atcatccgcg actgggatgc taaatggtat gctgaaaaag agtatgctga gttcttacac
     3901 gaagatttaa gaatccgtaa atttatttca actaaacttg ctgatgctgc tgtatcaaca
     3961 gttgaaatcg aacgcgcaac aggtcgcgta aacatttcaa ttcacacagc taaaccaggt
     4021 atggttattg gtaaaggcgg ttctgaagtc gaaagcctaa gaaaagaatt aaacaaatta
     4081 actggtaaaa gaatccatat caatatcgtg gaaatcaaaa aaccagatct agatgcaaaa
     4141 ttagtaggtg aaggaatcgc acgtcaatta gaaaaccgtg ttgctttccg tcgcgctcaa
     4201 aaacaagcca tccaacgctc aatgcgtgct ggcgctcaag ggatcaaaac tcaagtatct
     4261 ggtcgtctaa acggagcaga tatcgctcgt tcagaaggat actcagaagg aactgttcct
     4321 cttcatactt tacgtgcgga cattgattac gcatgggaag aagcagatac aacatacgga
     4381 aaactaggag ttaaagtgtg gatctatcgt ggagaaattc ttccaactaa aaaaaacact
     4441 gagaaaggag ggaaataatc atgttagtac ctaaacgtgt aaaacaccgt cgtgaattcc
     4501 gcggaaaaat gcgcggggaa gctaaaggcg gaaaagaagt agcattcggt gaatacggtt
     4561 tgcaagctgt tgattcacat tggatcacaa accgccaaat cgaagctgct cgtatcgcaa
     4621 tgactcgtta catgaaacgt ggtgggaaag tatggattaa aattttccct cacaaatctt
     4681 atactgccaa agcaattggg gtacgtatgg gttctggtaa aggggcacct gaaggatggg
     4741 ttgcaccagt aaaacgtggt aaaatcatgt ttgaaatcgc aggcgttcct gaagaagtag
     4801 ctcgtgaagc gttacgtcta gcttctcaca aattaccaat gaaaactaag atcgtaaaac
     4861 gtgaggaaat gggtggtgaa tcgaatgaag gttaaagaaa tcagagaatt aaccactgcc
     4921 gaaatgcttg atcaagaaaa acaattaaaa gaagaattgt ttaatcttag attccaatta
     4981 gcaacaggtc aattagaaaa cactgcacgt attaaagaag tacgtaaatc gattgcacgc
     5041 atcaaaactg tgttgcgtga acaagctaag taattatgga aggaggccat taagcgtatg
     5101 actgaaggaa gaaatcaacg caaagtttat caaggtcgcg tggtttcaga caaaatggat
     5161 aaaacaatta ccgttgctgt cgaaacaaag aaaaaccacc ctatctatgg caaacgcatg
     5221 aactattcta agaaatacaa agtgcatgat gaaaacaaca cagcaaaagt tggcgacatc
     5281 gtgagaatta tggaaactcg tccattatca gctacaaaac gtttccgttt gttggaagta
     5341 gtcgaagaag cagttattat ctaataacac gagattttcc tatatagatt gaatgcaaaa
     5401 tctgaaagga ggatacacaa cgtgatccaa gcagaaagtc gtttaaaagt cgctgataac
     5461 tcaggcgcac gtgaaatttt gacgatcaaa gtccttggtg gatctggtcg taaaactgct
     5521 aacatcggtg atgtgatcgt tgctacggtt aaacaagcaa cgccaggtgg ggttgtcaaa
     5581 aaaggtgacg tcgttaaagc cgttatcgtt cgaacaaaat caggagctcg tcgtacagac
     5641 ggttcatata ttaaatttga tgaaaatgct gcagtgatta ttcgtgatga taaaagccct
     5701 cgtggaacac gtatctttgg gccagttgca cgtgaactac gtgaaaacaa cttcatgaag
     5761 atcgtttcac tagcaccaga agttttataa tcttgagaca cttatcaaag gaggtgcgaa
     5821 acagttatgt ttgttaaaaa aggcgataaa gttaaagtta tcactggaaa agacaaaaac
     5881 aaagaaggcg ttgtactagc agcgttcccg aaaaaagata aagttattgt cgaaggtgta
     5941 aatgtcgtta aaaaacacca aaaaccttct caagctgctc ctcaaggtgg aattgttgag
     6001 atggaagcgc caattcatgt ttctaacgtg atggtggttg actctactgg tgtagctggc
     6061 cgagttggct acaaagaagt agacggcaaa aaagtccgtg tttctaaaaa aaccggtgaa
     6121 gttttagata aataaaagtt taaggaagga gggtcttact gaatgaaccg cctaaaagaa
     6181 aaatatctta aagaagtaac tccatcattg atggaaaaat ttaactatag ctctgttatg
     6241 caaacaccta aagttgaaaa aatcgtcatc aacatgggtg ttggtgatgc ggtatcaaac
     6301 gctaaaaact tagataaagc agttgaagaa ttaactttga tcactggtca aaaaccaatg
     6361 attacaaaag ctaaaaaatc aatcgctgga ttccgtttac gtgaaggaat gccaattggt
     6421 gcaaaagtta cattgcgcgg agaaagaatg tacgaattct tagacaaatt ggtttctgtt
     6481 tcactaccac gtgtacgtga cttccatggt gtaagtaaaa aagcctttga cggtcgtgga
     6541 aactatactt taggtattaa agaacaattg atcttcccag aagtagatta tgatttagta
     6601 gataaagtac gtggtatgga catcgttatt gtaacaacag caaactctga tgaagaatct
     6661 cgtgagttgt tgacacaatt aggcatgcca ttccaaaaat aattaaagga ggcgaactac
     6721 gtggctaaaa aatcaatgat tgctaaaaac aaacgtcctg caaaattttc aacacaagct
     6781 tatactcgtt gcgaacgttg cggacgtcca cattcagttt atcgtaagtt ccatctttgc
     6841 cgtatttgct tccgcgaact tgcctataaa ggtcaaattc ccggcgtgaa gaaagctagc
     6901 tggtaaaaag taaattcgca taagaaggag gtaaaagatc aatggtcatg acagatccaa
     6961 ttgcagattt tctaactcgt atccgtaacg caaacatggt taaacatgag tctttagaag
     7021 tacctgcatc aaaaattaaa cgtgacatcg ctgaaatctt gaaacgtgaa ggtttcgttc
     7081 gtgatgttga atacatcgaa gatgacaaac aaggcgtgat ccgtgttttc ctaaaatttg
     7141 gtaaaaacgg cgaacgtgtt atcacaaact taaaacgtat ctctaaacca ggtttgcgtg
     7201 cttatgtcaa agctgacgaa gtaccaaaag tattgaacgg tttaggtatc gcaatcattt
     7261 cgacttctga aggtgttgtc actgataaag aagctcgtga aaaaaatatc ggcggcgaag
     7321 ttatcgctta cgtatggtaa tcaagtagaa tctacaagga ggtgtcttta agtgagccgt
     7381 atcggaaata aagtcgttgt tatccctgaa ggagtaacag ttactcaaga cggaaacaac
     7441 gttacagtta aaggaccaaa aggtgaattg actcgtactt tttcagcaga tatcaaaatg
     7501 aatatcgaag gaaacgaagt aactttcact cgtccaaacg acagtaaaga aatgaaaaca
     7561 attcatggaa caactcgtgc gaacttcaac aacatggttg taggtgtcag cgaaggtttc
     7621 caaaaagcgt tagaactaat cggggttggg taccgtgcac aattacaagg atcaaaactt
     7681 gtattgaacg ttggttactc acatccagta gaaatcacac caccagctgg agtaactgtt
     7741 gaagtaccag ctaacacaca agtaattgtt aaaggtgcga acaaagaaga agttggcgaa
     7801 ttagctgcaa acattcgtgg aactcgtcct ccagaacctt ataaaggtaa aggtattcgt
     7861 tatgttggcg aatatgtacg ccgtaaagaa ggtaaaactg gtaaataatc gtttagaaaa
     7921 attgcataga agccagcagc aggaaaaagg catttggctg aagtttacct tcaaaaaaat
     7981 acatttttac ttgtgccgca gcttctattt ttcgaaacgt atttactgct tgaaatagta
     8041 gtacaataaa aataaagagg tgacaattgt gattacaaaa ccagataaga acaaaacacg
     8101 tcaaaagaga catcgtcgtg tacgtaacaa tatctctggt actgctgagc gcccacgctt
     8161 gaacgttttt cgttctaaca aaaacatcta cgctcaaatt attgatgacg tagcgggtgt
     8221 aacgctagca agtgcctcta ccttggataa agaaatttca ggtggaacaa aaacagaagc
     8281 agcacaagct gtcggtaaac tagttgctga acgtgcagct gaaaaaggca ttaaagtagt
     8341 agtctttgac cgtggtggat acctttacca tggccgtgtg caagctttag ctgaagctgc
     8401 tcgcgaaaat ggactagaat tttagaagaa ggaggaatac cattcatggt ttatatcgat
     8461 ccaaaacatt tggaattaga agaccgcgtt gttgcaatta accgtgtaac aaaagttgtt
     8521 aaaggtggac gtcgtctacg ttttgctgct ttagttgttg tcggtgacaa aaacggacac
     8581 gtaggatttg gtacaggtaa agctcaagaa gttcctgaag caatccgtaa agcaattgaa
     8641 gatgcgaaga aaaacttagt tgaagtacct atggttggtt caacaatccc acacgaagta
     8701 atcggtgctt ttagtggtgg tagaatccta atgaaacctg ctgtagaagg ttctggggtt
     8761 gctgctggtg gaccagttcg tgccgtatta gaattagctg gggtagcaga tatcacttct
     8821 aaatctttag gctcaaatac accaatcaac gttgttcgcg caacagttga aggtttgaaa
     8881 caattgaaac gtgcagaaga agttgcagaa cttcgcggca aatcagttga agaattaatc
     8941 ggataaggag gacaacaata atggctgaat taaaagttac tttaaaacgc agtattatcg
     9001 gacgtcctca aaaccaacgc gatacagtta aagcgttagg tcttacaaag attaacagtt
     9061 cagtggttaa gccttctaat gaagcaatta aaggcatgat caatactgtt tctcacctag
     9121 tggacgttga agaaatttaa tttagaaaaa tcataactag aacattaagg aggtgccaaa
     9181 cctatgaaac ttcatgaatt aaaacctgct gaaggttcac gccaagtacg taaccgtgtt
     9241 ggtcgtggta cttcatctgg taacggaaaa actgccggac gcggacaaaa aggacaaaaa
     9301 gctcgttcag gcggcggtgt gcgtttagga tttgaagggg gacaaacacc attgttccgt
     9361 cgtttaccaa aacgaggatt cacaaacgtg aaccgtaaag actatgcagt cattaatctt
     9421 gatgtattga accgctttga agacggagct gaagtaactc ctgttgcttt agttgaagct
     9481 ggaatcgtga aaaacgaaaa agctggaatc aaagtattag ctaatgggga actaaacaag
     9541 aaattaactg tgaaagcagc aaaattctct aaagcagctc aagaagcaat tgaagcggct
     9601 ggtggatcaa tcgaggtgat ctaatgttta gactattaaa agatgctttt aaagtaaaag
     9661 acattagatc gaaaatccta tttactgtat ttgtgttgtt tgtatttcgc ctaggtgcac
     9721 atattactgt accaggcgta aatgctgctt cattgcaaaa cctttcttct ttgccatttt
     9781 tcaacatgtt gaacttggta agtggtagtg cgatgcaaaa cttttccatt ttctcaatgg
     9841 gggtttcacc atacattact gcttctatcg tcattcagct tcttcaaatg gacatcgtgc
     9901 ctcgatttgt tgaatggtcg aaacaagggg aagttggtcg taaaaaactg aatcaagcga
     9961 ctcgatatct gacattagta ttggcattta tccaatctgt cggtatcact gctggattca
    10021 atcaatacgc tcaattaggt ttcgtgaata atcctaacgt agcaacttac gtaacgatcg
    10081 gtttgattct aacagccggt acgatgtttg ttacttggct aggagaacag atcacagaaa
    10141 aaggtatcgg aaatggcgta tcaatgatca tttttgcagg tatcatttct agattgcctg
    10201 aaagtatcag agaaatttat gaagattact ttataaatat tgatccttca gacatatgga
    10261 aatctgctat ttttgtggcc attttactag tagcggtctt gttgatcgtg acattcgtaa
    10321 catttttcca acaagctgaa cgcaagatcc cgatccaata tacgaaacgc gtagctggcg
    10381 cgcctacaag cagctatctg ccgttgaaag taaatgcagc tggagttatt ccagttatct
    10441 ttgctagttc gtttattgct acgccaaatg ctgttttaca agcattgagc ggtagattcg
    10501 gttctgaaaa ctggtacaaa gtgatgatgg agatctttaa ctataataca gcacctggtg
    10561 ctacgattta tacgattctg atcgttgcct tcactttctt ctacgccttt gttcaagtaa
    10621 accctgagaa attagcggaa aacttgcaaa agcaaggaag ttatatacca agcgtacgcc
    10681 ctggtaaggg gactgaagag tatgtctcaa gattactgat gcgtttaagt acagttggtg
    10741 cagtcttcct tggtttagtg gcattgcttc caatcattgc tcaaatggtt tgggatcttc
    10801 cacaatcaat cggattaggc ggtacaagct tactgatcgt tatcggtgta gctttagaaa
    10861 ctgcgaaaca gttagaaggc ttaatgctga aacgtaaata tgttggattc attgagtaag
    10921 aaagcgtgtt gaggtttccc ctcaacacac gttctcacac atttattagg agggaaaaca
    10981 atgaacctca ttttaatggg gctgccaggc gcaggtaaag gaacacaagc tgaaaaaatc
    11041 attgatgctt atggtattcc gcacatctca acaggagata tgttccgcgc agcgatgcaa
    11101 aacgaaacag ctcttggcct ggaagcaaag tcttatatgg ataaaggtgc attagttcct
    11161 gatgaagtaa caaatggaat cgtgaaagag cgactagctg aaccagatac agagaaaggc
    11221 ttcttattag atggctttcc acgtacgcta gatcaagcaa aagctctgga cgcaatgcta
    11281 aaagatttga ataaaaaaat tgatgctgtc atcgatatcc atgttggtga agaaatcttg
    11341 gtcgaacgtc tagccggccg atttatctgc agcaattgtg gagcaactta ccataaaatt
    11401 ttcaacccta caaaagttga agacacatgt gatcgttgcg gtgggcatga attctatcaa
    11461 agagaagatg ataaacctga gacggttaaa aatcgtctgg ctatcaacat caaaaatagt
    11521 gaaccaatct tggcttatta taaagaacaa ggtttgctaa acactattga tggtgctcgt
    11581 gaaatcgatg ctgtatttgc agatgtacaa aaaatcattg aaaaatagca cgattgcccg
    11641 tagccgactt gcattatgca gttactcttg tcaagttcaa taaattatga tagaatatgt
    11701 cagtccgact tctagagaat cctctagatg atccattttg agaattttga ggtgggaact
    11761 gcagtttccg ccattttatc gtgtgaaaat gcgaaacaag tacaaggagg tactgtgcgt
    11821 ggcaaaagaa gatatgattg aagtcgaagg tacagtcgtc gaaactttgc cgaatgcaat
    11881 gtttaaagtt gaactagaaa acggacacca agttcttgct actgtttcag gtaaaattcg
    11941 tatgcattac atccgtatct tacctggtga caaagtaacg gttgaattgt ctccatacga
    12001 tcttaaccgt ggccgtatta cttatcgctt taaataattg tactccgtaa aactacagga
    12061 ggtattatca tgaaagtaag accatcagta aaaccaatgt gcgaacattg taaagtaatt
    12121 cgtcgtaaag gacgcgttat ggtgatttgc ccagcaaatc caaaacataa acaacgtcaa
    12181 ggataattgg aggtgctata aatatggctc gtattgcagg agtagatatt cctcgtgata
    12241 aacgtgtagt agtttcactt acttatatct atggtattgg taacactact gctaaaaaga
    12301 tcttagctga tgctggtgta tctgaagatg ttcgcgttcg tgaattaacg aatgaacaaa
    12361 cagatgctat ccgtgcagaa gtagataaat taaaagttga aggggatctt cgtcgtgaag
    12421 tgaacttgaa catcaaacgt ttgatggaaa tcggttctta ccgtggaatc cgtcatcgcc
    12481 gtggattgcc tgttcgtgga caaaacacga aaaacaatgc acgtactcgt aaaggcccag
    12541 ctcgcgctat cgctggcaag aaaaaataat tcttaagtga aggaggttaa aacttcatgg
    12601 cagcaaaaaa agtgaatcgt aaacgccgtg tgaagaagaa tatagaagca ggtattgcac
    12661 atatccattc tacattcaat aacactatca tcatgatcac tgatactcac ggaaatgcat
    12721 tagcgtggtc atcagctggt tcattaggtt tcaaaggaag caaaaaatca actccttttg
    12781 ccgctcaaat ggcagcagaa actgctacaa aagcagcaat ggaacacgga ttaaagactg
    12841 ttgaagtaac agttaaagga cctggttctg gacgtgaagc agcaattcgt tcattacaag
    12901 ctacaggttt agaagtgact gcaattcgtg acgtgactcc agttcctcat aatggtgccc
    12961 gccctccaaa acgccgtcgt gtttaatgag tgccgctcat tttgagtttt atcagtaacg
    13021 tcattgaaca agacactttt cgttttgaaa ggggtaaaga taagaatgat tgagttcgaa
    13081 aaaccaagaa tcgcaaaaat tgatgaagaa aaagattatg gcaagttcat cgttgaacct
    13141 cttgaaagag ggtatgggac tactttaggg aattccctac gtcgtatttt attatcttct
    13201 ttgccaggtg ctgccatcac taatattcaa atcgatggtg tactgcatga attctctacc
    13261 atcaaaggtg ttagagaaga cgtcactcaa atcatcttga acattaaagg cttagcgctg
    13321 aaaatgtatg gccaagaaga aaaaaccctt gaaatcgata ttaccggacc tgctacagta
    13381 actgctggcg atattatcgt tgatagtgat gtagaaattc ttaacaaaga tatgtacatc
    13441 tgtagtgtct ctgaaggagc tacgttccat gctcgcttaa cagtgaaacc tggacgtggt
    13501 tatgttcaag cagatgaaaa taaaaaagaa gatatgccaa ttggcgttct tccagtcgat
    13561 tcaatctaca cacctgttcg tcgtgtgaac tatcaagttg agaacacacg tgttggacac
    13621 cgtgaagatt ttgataaatt gacgatggag atttggacag atggttcaat tgaaccgttg
    13681 gaagcaatga gtttgtctgc gaaaatcatg actgaacatt tggatatttt tgtgaactta
    13741 actgatgaag caaaaaatgc tgaaatcatg gtcgaaaaag aagaaacaca aaaagaaaaa
    13801 atgttggaaa tgacgatcga agagttagac ttgtctgttc gctcttacaa ttgtttgaaa
    13861 cgtgcaggaa tcaatactgt acaagaactt acaaataaat ctgagcctga aatgatcaaa
    13921 gtccgtaact taggacgtaa atcattagaa gaagttaaag caaaattgca cgacttaggt
    13981 ttaggtttac gtaaagaaga ttaaaacctt ttacgaagga gggaaaccaa cgtgagttat
    14041 cgtaaactag gacgcacatc tagccaacgt aaagcgatgt tgcgtgattt aacaactgat
    14101 ttattaatca acgaacgtat tgtgactact gaagctcgtg cgaaagaagt tcgctcaact
    14161 gcagaaaaaa tgatcacttt aggtaaacgc ggagatttgc atgcacgtcg tcaagctgcg
    14221 gctttcgtac gtaatgaagt tgccagcgtg cgtgaagaaa atgaggacat cgtcattgaa
    14281 tcagctttac aaaaactgtt caacgatatt gcacctcgtt acgcagaacg tcaaggcgga
    14341 tacacacgca ttttgaagac agaaccaaga cgcggtgatg gtgcaccaat ggttgtctta
    14401 gaacttgtct aaaatcacac taataacatc actttataga tgattctttt agagtgttat
    14461 gatgttggaa gatatttttc cgagtctagc tcagcgctgc tcctcaagcg aatatcgctt
    14521 ggggagtaag cactcatcat aaagtgtttt tttatacctt caattacctg tcatgacaga
    14581 tatgtttgaa ggcataaaaa aacattttaa gcgagaaata attgaaaatt ttgtcttttt
    14641 ttgatagtgt tagtaggaac tttaggatga agaaggagta atcatggagc caattattga
    14701 attagaaaag ataaattata aataccagcc agatgatctt cgccccgcat tgaaagatgt
    14761 ctcctttaca attgataaag gtgagtggat cgctattatt ggacacaatg gttcaggaaa
    14821 atcgacactt gccaaaacga tcaatggatt gcttctgcca gaatcaggga tcgtgaaagt
    14881 gggaaatcaa atattggatg aagaaaatat ttggacgatc cgacagatgg ttggaatggt
    14941 ttttcaaaat ccagacaatc agtttgttgg ttcgacagta gaagatgacg ttgcttttgg
    15001 tcttgaaaat caagggattc ccagagaaga aatgctagtg cgtgtgaaag atgcacttga
    15061 gaaagttaga atggctgagt ttgcttcacg cgagcctgct cgtttatcag gagggcaaaa
    15121 acaacgtgtt gccattgccg gcgtggttgc cttacgacct gatatcatta ttttagatga
    15181 agcaaccagt atgctagatc cagaaggtcg tgaagaagtg atctctacga tcaagaagat
    15241 caaagaagaa agtcaattga ccgttatatc tatcactcat gatattgatg aagcagctaa
    15301 tgccaatcgg atactagtga tgagacaagg cgaacttgtc cgtgaaggta caccaaaaga
    15361 aattttttct gctggtcctg aactgatcga cttaggtcta gatctaccat ttcctgaaaa
    15421 actgaagagc gcactgaaag aacgtggggt tgatgtgccc agtgaatata tgacagaaga
    15481 aaggatggtg gattggttat ggacatccgt tttgaacaag tagacttcac ttatcagccg
    15541 aatacaccat ttgaacaaag agccttattt gacatcaata tgacgatcaa agaaaacagt
    15601 tatactgcat tagtagggca taccggaagc gggaaatcaa ccttacttca gcatttgaat
    15661 gcgcttgtta agccaacaag cggtacagtc catattggag aacgagatat tcagccggat
    15721 acggataata agaatttaaa gcccatccga aaaaaagttg gcatcgtctt tcagtttcca
    15781 gaagcacagc tattcgaaga aacggtagca aaagacattg ctttcggtcc taaaaacttt
    15841 ggtgtcagcg aagaagaagc actagtccta gcaaaagaaa cattagaaca agttgggctg
    15901 gatgaaagct atttggaacg ttcgccattt gaactttcag gagggcaaat gcgtagagta
    15961 gcaatcgctg gtgtgcttgc catgaggccg gaagtacttg tattggatga accaacggca
    16021 ggacttgatc cccagggcag aaaagaaatg atggagatgt tttggcgtct tcataaggaa
    16081 cagcagatca ctattgtatt agttacgcat ttaatggatg atgtagccaa ttttgcagat
    16141 tatgtctatg tgttggaaaa gggacgaatc gttaatagcg gggagccgca agaggtcttt
    16201 caaaacattg aatggctaaa agaaaaacaa ctaggcgtac ctactgccac cgagtttgca
    16261 gaagaattaa tggctaaagg gatgaacttt gctaccttgc ctttgacagc tgaagaatta
    16321 gcagacgcta tcgtccatta tgctggagga aatgcccatg atgaataaat tgatatttgg
    16381 tcgttatatg cctggagatt ccttgatcca tcgattggat ccccgagcta aattattagc
    16441 cagtttttat tttatcggaa tcattttttt agcaaataat tgggaaacgt accttttgtt
    16501 ggcatttttc acattacttt ctgtcttctt gtcaaaagta gatttgaaat tttttatccg
    16561 aggcattcgc ccattgatct ggctaatcct cttcactgta gctttgcagg tcttgtttac
    16621 aagtggcgga gaaacttatt ggagttgggg tgttttccat atcactgaat ttggtgtgat
    16681 gaacggcctc tttattttct gtcgattcgt gttgatcatt tttatgtcga ctctactcac
    16741 gcttactaca ccgcctttgg aattatcgga tgcgattgag tacttgcttc gtccattgaa
    16801 agccgtacgg ttcccagtcc acgaaatttc attgatgctg tcgattgctc ttcgttttgt
    16861 gcctacattg atggacgaaa cagaaaagat catgaacgct cagcgcgctc gcggtgttga
    16921 ttttggtgaa ggaaacctag ttcagaaaat gaaagcagtc attcctttgc tgattccttt
    16981 atttgtcagc agcttcaacc gtgcggaaga tcttgcgact gctatggaag caagaggcta
    17041 caaaggtggg gaaggaagaa cgaaatatcg tgtattgcat tggcataata gagacacgat
    17101 ggttatcatt gcttttgtat tattaactgt gctactgatc tttttaagag gataaacgtt
    17161 cagtgggccg ctatctattc ttttcttgct gatctgtttg caagaaagga tgcaagcagt
    17221 gtatgacaag aaatattagg gtctgtggca gactaaggtc acaggcctct ttcttttatc
    17281 tgaaaagaaa gaacgaaaaa attttataga aaaatcatta ttccagataa ccttttagga
    17341 acaaaggaga gaaaaacatg gtacgttaca aagcaatcat tgcgtacgac ggaacaaatt
    17401 ttaatggatt ccaaaaacag ccaaatgggc gtactgttca agaagaagta gaaaaaacat
    17461 tacaaaaaat ggcaaatgga aaagaaatca cggtctttgg ttcgggaagg acagatgccg
    17521 gagtacatgc gatcgggcaa gtaatccatt ttgattatcc agaggagcgt ccgttagaac
    17581 gaatgcgttt cgcgctagat acccagtcac cagaggatat tgctgtaaga caagtagaaa
    17641 ttgtttcaga agacttccac gcaagatatt tggtcaaaga aaagacgtat caatttcgag
    17701 tggatatagg gaaaccacgc agtccttttc gacgccatta tgcaagttat tttccttacc
    17761 cacttgattt ggaaaaaatt cagcgggcgc ttcctgattt attagggact cacgatttca
    17821 cctctttttg tgcatcagga agctcgatag aggataaaat acgtacgatc tacgaggcta
    17881 agatggaggt aaatgaaaca aaagacgaat tgttgtttac atttagggga aatggcttct
    17941 tgtataagat gatacggatc ttagtaggaa cgcttttgaa aattggcaat ggccgattgc
    18001 ctgaagatag tatcccagat attattgcga aaaaagatcg taatgcagca ggacctacag
    18061 cacaccctga aggactttat ttgtatgaag tgaaatatga ataagaggtt atgatagaag
    18121 tggtcagctt caagaaataa gaaggtattt acgaaaatta cttctcaaaa ttttgcaaaa
    18181 tttcggctta ttttcgaaga agctacttct ggaacaccgt ttaaggaaga ggtcgtgaaa
    18241 tcagcgtttt gacctgagta ttagagaaac aaacgggaaa atagctcctc atattttgcc
    18301 gtttgttgag ctaataccga aggtcagctg attgaacacc gtttaagaaa gaggtcgtga
    18361 gcctgtcatt taactctgag tcacaaagag caatttcaaa aaacagcttc tcatctttga
    18421 tgaatatcgt gacttgtgga cgaggcgttg ataagtgaat catctttttg ttacctattt
    18481 tttctgtaac caaaaaacga tggaacgttg ttttgattgc ccttggtgcg gacagtctga
    18541 acaggttcca tcgtttcttt attttctacg tcttcttctg tgtgtgtttc gtggttgttc
    18601 ttctattgct ggatcaatcg tgccataaaa ggggcgattg cggtcaaggt accagacata
    18661 aagaataaac aatgcaatac ataggacaaa aaggattgca cctatcgtaa atccttcagg
    18721 caagtaactg aaagtgacag tatgtttacc tgctggaact tttatcatca agaatgcgtc
    18781 tttgaaattg ctgaccttga ctggtttccc atcaatttta gctttccatc ctttgtcata
    18841 aggaatagtt gtcaccaaca tggaatcttc agcggtattt acagtcccag aagcttttct
    18901 gccagtggct tccatttcga cacctttttc tttgatctga ttcattgcgg cttgataggc
    18961 tacaacatct agaccaacga ctttaggctc catgaaactt atttctttcg tcccataaaa
    19021 acttgcagta aatgtcactg ttgtctcttg aggataatag ccaagatcat agtattgacc
    19081 agaaatgttg atttgcgttt ttcgcgatgt accattgaca gtgatcgtcg cactagaact
    19141 ttctaattgc gcaaaattgg ttgggaacaa acttaagtaa gcttgtgtat ttgcaggcac
    19201 atggacttcc caagtgagct cttttggctg gttatctgct gtttctgagt aagtgacgcc
    19261 agtcgttgtc tttgtgatcg tcgtattcaa ctgattcttc aaagtaggtg tatagaatgt
    19321 aaaatactgt tcatttagac cagacaatgt attgatcaag tttgtctgac tggtcaaatt
    19381 atctgtcact ggctgctcaa catcattaac ggacaacggt gcagtaaatg ctagtggcaa
    19441 tgcattgaca ttctgataca gtgtatagtc gccgctttga ccagcttggc ggaagccata
    19501 tttactgaac gtgctttcgt ttttagaaat gttatatttg attcctgtaa acccatccat
    19561 cagcaaagta ttattgttat aacggatatt caaatttgtc cctcttgaac ggaaacctaa
    19621 tttatccagg taagaggaag aattacgatt tcttatagaa gaaaacaaac tgataccact
    19681 gtaaccatag ttgatcgaat cgtttgcaga tactggatcc agattttcca agcggtaaaa
    19741 cgagtcggaa tctttttttg tttcatttac aagagctttc aagtcaggat aaggatcagt
    19801 atataaactg cgggaagcat aattccaatc atcaagaatg ccatggatca tactgttcgt
    19861 attgaccagt gcttcagcag acatgatcaa taataacaat atactcagat gattgaatga
    19921 aagtttttta tgctgataaa atcccacagc aaaagcataa atccctaaga aaaccactgt
    19981 caatacaaag gaagtcatcg tcacataggt gtagcttcct tcaggtttga tgccccatgc
    20041 caccgcaaag agagcagcta cgaccaaaat gattcctaac aacagcccac gatcgtcttg
    20101 ttctatcttt tcccatcctt ttcctgcaag caggagcact aagaaagaga acagataggc
    20161 ataacggaat aaaaacatat tcggtgcatg cataccatgc cagaacaaat tcaatggaac
    20221 aagataaaaa cttgcgataa gtatggcgaa aagactgccg tacaaaaatt tatttttcaa
    20281 cggtactttc cgactggcaa aataaaacag acagaggact aatggtagca gacctgcgta
    20341 aacgaacgga atcgatccgt attttgtcgt gtcataaaca ccaatcatat ttttcatgat
    20401 tagatccaga taagaagtcg cttctgtttt caacgtagtg atctgactca gtgcctcgcc
    20461 atttgaccga agatccagca ctgctggcaa tacaatgatc attgaagcgc cgcctgccag
    20521 taaagaagtg ataccataag gaacaatcgt ttttttatat tctgaccagt ttgtcaatag
    20581 acgggcgata aaatataaga aagaaaatac gccaatcata aaacccatat aaaaactgga
    20641 taaaaacaag agtaaataac tgataaataa aagtttcggc cgtttaagat ccataacacg
    20701 atgaatcccc aaaatcacta atggaagata aaaaaatgca tccagccaca tgatgatctc
    20761 cgaatgtgca gtgataaatg agatcaatgc gtaactgacg ctaagtgcaa catgccccca
    20821 gcgaggaatc ttgaacgtac gtgcaagaaa ccaaaaagcc aatccggcac agccgacctt
    20881 aagcaaagtc agaacataca gtgcatctgg catcatctgg tttggaaaaa agaacaccag
    20941 tggcgtgaag atcccgccta gatagtaaga aattaaagat aaataattca atcctagaga
    21001 agcattccat gtataaaaaa tactttgctt tccaagaaga acatttcgga agctggcatg
    21061 aaaattagaa aactgtgaaa aggcatcact tgccaaaata cttctgctgc ttccaggata
    21121 gattccgata cttagataaa caagtgctaa aatcaaaaac ggaatcagga aactagctgc
    21181 tgcgtagcgt ccgttttctc taaagaatga ctttaaattt tttttcatat ttattttcca
    21241 ccaaatttct atgaagttgt attcagttta tcatatcctt gtttttctct gcttaaaaag
    21301 agtctagttt tcacaaaaaa ataaaattta gaataaataa cttcataatt tgttcgtata
    21361 acaaaaataa acgcaaaaaa agggtaggaa tcaaaagtcc cacccttaaa aatttttatt
    21421 aaataaaaaa ccgttcacta tctgaacggt ccaagtatca ttcaattaaa cgcgttcaac
    21481 ttttcctgat ttcaaagcac gagttgacac ccaaactttt ttaggttttc cgtcgaccat
    21541 aacgcgaact ttttgtaagt taggtttaac agtacgtttt gttgagttca tagcgtgtga
    21601 acggttgttt ccactttttg tcttgcgacc tgtaaagtaa caaacttttg ccatggtaat
    21661 ttcctccttt gccttgattt ggagcaatag aataattcag ctcataccag actaacatat
    21721 catatagaat gggaatttgc aagtacattt tcatagatac aattgacaat tgagaagaat
    21781 aattgtaaat taaacaaggt tatacctgaa attttgcaag gtttttgagt actttgggta
    21841 actgcctttt atttcaagtt gcgctatgat aaaatatgat agaagaaaaa tgggagtttt
    21901 ttacccgtta aggaggctca ttcatggctg taaaaattca aacgtcagct ggaaccattg
    21961 aaattaccaa tgatgtaatt gcaacagttg ttggtggcgc cgcgacagat gtgttcggtg
    22021 ttgtaggaat ggccagcaaa aatcaaatta aagataatat tactgagatt ttgcgtaaag
    22081 aaaattactc aagaggagta gttgtccgcc aagaagataa tggtattgct gtagatgttt
    22141 atacgattat cagctatggt acgaaaatct ctgaagtttc ccgcaacgtc caagaaaaag
    22201 tgaaatacaa tctagaggca ttattaggcg taactgccaa ttcagttaat gtctttgtac
    22261 aaggtgttcg tgtactgcca gactaggcag gagactggct ggtaactcag cagtcgatct
    22321 gaaggaggat tatctaggtg agtgtaacag aaataagtgc aagccagttc caggaaatgg
    22381 tccaggcagg tgcgaatcgt ctgcaagtaa atgcagaata cgtcaattca ttaaatgtat
    22441 tccctgtgcc agatggcgat acgggaacaa atatgaatct atctatgact agtggagcta
    22501 aggcagtagt agattcaacc tcagaaaaag taggagaatt agcagcaatg ctttcaaaag
    22561 gattgctgat gggtgcgcgc ggaaattccg gcgttatttt gtctcaattg tttcgtggat
    22621 tttctaagca aattccagat gttgtctcat taaatgcttc tgatcttgct gcagccttca
    22681 ctcatggagt tgaaactgcg tataaagcag taatgaaacc agtagaagga acgattttga
    22741 cagtagctag agaagcagcg aaagctggag aaaagaaagc aaaaagtact gatgatgtcg
    22801 ttgaagtaat gacagctgtt gtaaaaggag caaagcgtgc tttagctaaa actccagact
    22861 tgcttccagt cctaaaagaa gtcggtgtcg tagacagtgg agggcaagga ctgctgtttg
    22921 tttatgaggg ctttttaagt gcattgaatg gagaattcca agcggatgaa acatatgaac
    22981 cttctccagc agaaatggat gatatggtca atgcagaaca tcatcgcagt gtccaaggtc
    23041 aaatggcgac agaagatatc cattttggtt actgtacaga aatcatggta aaaatcggag
    23101 aaggacctac agtcgatagt acattcgatt acgaagaatt ccgtaactat ttagatggta
    23161 taggagattc tttacttgtg gtcaatgatg acgagatcat caaagtccac gtgcatacag
    23221 aaaacccagg agaagtcttg aattatggac aaaaattcgg ttcattagtc aaaatcaaag
    23281 tcgacaacat gcgcttgcag catgagacga ttttggaaca cgatgaagaa gtgaacgagt
    23341 ttgcgaaaaa agaaatacca cgtatcccgt atggtatcgt agcgatcgct gctggtaaag
    23401 gcgtccaaga gctgtttgaa agtttaggcg ctaattatgt tatcagtggt ggacaaacaa
    23461 tgaatccaag tacggaagac attgttaaag cgattcaaga agtaaacgca gataaagtga
    23521 ttgtcttgcc aaacaataag aacatcttca tggcagctga tcaagcagca gaagtagcag
    23581 atatcccagt agcagttgtt ccttcaagaa cgatttccca aggattgaca gctatgcttt
    23641 cattcaatga tcaagcttct ttagaagaaa acaaagaaac gatgacagaa atgttgtcta
    23701 gcgttgtgag cggtcaagtc actcacgcga tcagagatac ctcaattgac ggtgtagaga
    23761 tcaaagaagg agactttttg ggcatgatcg atggaaaaat cctgatctct aacccagata
    23821 tcacagcaac agcgttaagt acactagaac aaatgatcaa tgaggacaca gagatcgtaa
    23881 cgatcttggc aggtgatgaa ggaacagttg aacaagcaaa tgaatttgct gagaaactaa
    23941 cagaaaaaca tcctgatttg gaaatcgaga tccaccaagg agatcagcct gtctatcctt
    24001 atctattttc tgctgaataa tttctgtgtc agaatatctt tctaacacag tattcattca
    24061 taaaaggcct tcggttagtg atcaggtatg atcgctgacc aagtcctttt tttgttaaag
    24121 gagggaggaa aaatttatgc gttctttaga tgatccaatc actatgctgg caggagtcgg
    24181 agaaaaaaga gcagctagtc ttgccagctt gggcatccaa acgattgaag acttactgac
    24241 ttattatcct tttcgctacg aagatattca ggaaagaaat ctaaacgaga tccaggatca
    24301 agaaaaggtt actttgaaag gaatcgtagt ctcgcctcct gtgatgaatc gatttggtta
    24361 taaaaaaagc cgattgcaat ttcgtatgat gcaagaacat gatgtgttca acgtttcttt
    24421 ttttaatcaa ccttatctaa aagaaaaagt cattttatcg gaggaaatcg ccgtatacgg
    24481 taaatgggat gcaaagcgta aagccttgaa cgggatgaag attttaggtt ctcaaacaat
    24541 ggatgacttt tcgccgatat atcacgtcaa caaatcaatt cgtcaaacga cattagttga
    24601 tctaatcaaa aaagggtttc aagaatacgg cacattgatt gaagaaaatc tgccaaacaa
    24661 ccttgtggag aaatatcgac tgcttgaccg agcttctgct gtaagaagta tgcattttcc
    24721 aaaagaccac gaagaaaacc atcaagccaa acgacgtgtc gtttttgaag agttcttttt
    24781 atttcaaatg cgtcttcaag gattgaaaaa agcagaaaaa gcagaaacga atggtttgga
    24841 gatcctttat gatgtccaaa aattgaaaca atttactcaa aagctacctt ttgaactgac
    24901 aaatgcacaa aaacgtgtaa cgaatgaaat ctgtcgggat cttcgcagtc cgcagcatat
    24961 gcagcgactt ttgcagggag atgtaggtag cggaaaaaca gtggttgccg cgattgcgtt
    25021 atacgctacc gttacagcag gtttccaagg tgcgttgatg gtgcctacag aaatcttggc
    25081 acaacaacat atggaaagct tgcaacagct gtttgatccc aatgaagtaa ccatcgcatt
    25141 gattacgggg tcaacgaaag caaaagaacg acgtgagctc cttgaaaaac tagagcaagg
    25201 ggaaattaac gtcgtgatcg gcacacatgc attgatccaa gatggagtcg aatttcagaa
    25261 tttaggtcta gttattacgg atgagcagca ccgttttggc gtgaaccagc ggaaagtact
    25321 tcgtgaaaaa ggctggcgac cagatgtctt atttatgaca gcgactccga ttcctagaac
    25381 attagctatt acagcatatg gtgagatgga tgtatctatc attgatgaac ttccagctgg
    25441 acgtatcccg atcgaaacac gctgggtaag aacacctcaa ttggacagtg tactggattg
    25501 gacgaaaaaa gaactggcaa gaggacatca aatgtatgtc atctgtccgc tgattgaaga
    25561 atcagaagca ttagatgtaa aaaatgcagt ggaaatctat gaaaaacttc gtgaactatt
    25621 tgcaccaaaa tatgaagttg gcttgttaca tggtaaaatg aagaatcagg aaaaagaagt
    25681 aatcatggaa gccttcaaag acaaccagat gcagatatta gtttcgacta ctgtaattga
    25741 agtaggggta aacgtgccga acgcgaccgt catgctgatc atggatgcgg atcgatttgg
    25801 attggcacag ctgcaccaac ttcgtggacg tgttggacgc ggaagtgatg cttcctactg
    25861 tatcttagtt gctaatccta aaaatgaact aggtgtggaa cggatgaaaa tcatgacaga
    25921 gaccaccaat ggttttgtac taagcgaaaa agatctggaa ttacgtggtc ctggtgaagt
    25981 atttggcttc cgtcaatctg gtctgcctca atttgcgatt gctgacttgg tgacggatgg
    26041 aaatgttttg gaggtagcca gagaagaggc tgcagccctt tggcaaataa aggattggca
    26101 gatgcttcct gagtatcagc ctttagcgca aagcctccaa atagaaaatc aggataatcg
    26161 ttatttcgat tagcagactg tgctatactt tatactgatg acggaaaaag gagtggaaac
    26221 aatgaaaatt gccgtagatg caatgggcgg agatcacgca cctcaagcaa ttgtagaagg
    26281 cgtggcttta gcgcagcaag attttccaga agtagaattc ttactttacg gaaaagaagc
    26341 agaaatcaag aaatacttaa caaatgaaaa aaacatcacg attatccata cagacgaaaa
    26401 aatcaatagt gatgatgagc cagtaaaagc aattcgacga aaaaagactg catcaatggt
    26461 ccttgctgca caagcagtga aaaacggtga agcagatgct gtattttcag cagggaatac
    26521 aggcgcgcta ctagcagcag ggttgttcat cgtaggacgt atcaaaaata tcgaacgccc
    26581 tggcttgatg tctaccttgc cagtagtagg gaaagaaggt gccggtttcg atatgctgga
    26641 tcttggcgct aacgcagaaa acaaaccaga acatttgttg caatacggta ttttaggttc
    26701 tttttatgca cgtaaagtcc gaggtatcgg acgtcctcgt gtcgcactct taaacaacgg
    26761 aacagaagca acaaaaggta gtgaagtgac gaaaaaagcc tatgaactcc ttcagaatga
    26821 aacaagcttg aattttattg gaaacgtaga agctcgtgag ctactcaatg gtgtggctga
    26881 tgtagtggtc acagatggat ttacaggcaa cgctgttttg aaatctattg aaggaacagc
    26941 aatgaatatg atgaatctat taaaatctgc tattctaaat gaagggatca aaggaaaaat
    27001 gggcgctatg cttttaaaag acggcttacg ttctttgaaa gcagaaatgg attattccaa
    27061 acatggcggt gccgttttgt ttggcttgaa agcaccagtc attaaaactc atggagcaac
    27121 aggccccgat gctgttcgct atacgatccg ccaaatccac acaatgcttg agacagatgt
    27181 agttggacaa ttagtcgaac aatttgaaaa aagcgaagaa tagtatttgg atatctgaag
    27241 cgatccgttc cattgaaaaa aaggtggaca ttcctgaaaa atgccagtaa aattagccat
    27301 ggtgtaggta attgacaagt gtatgattct tggaggtgaa aaagttgact cgcgaagaag
    27361 tatttaacaa agtagcgaaa attatttcaa atcattttga gatagatacc gataaagtga
    27421 cggatgaatt gaacatcaaa gatgatttaa aagctgattc aattagtatt atggaattcg
    27481 ttttagagct tgaagatgaa ttcggtacgg aaatttcaga tgaagacgct gaacaaattg
    27541 aaacagtcgg tggcgcagta gattatatca gcagccatct taaataattt caaataaaag
    27601 caaagaagga taaaaagctg catgcagctt tttatccttc tttttttaat ctttttgaca
    27661 cgtaaaagtc atttttctga taattctggc gataaacaga aattatgttg caaagtaaag
    27721 cggttttcta tataattatt aataattcat tacacaaaga taagaataac ctttgttata
    27781 aaacgattta ttctgtgtaa tgtccttttt tattaagaaa aacttccatt taggaagcag
    27841 agaggggtat tcgattgtcc gataaccaat tattagaagt tcagaaccta catacaggat
    27901 tccgactcaa agatgactat tacgatgcag tagatgacgt ttcatttact ttagacaaaa
    27961 acgagatttt agcaatcgtt ggggaatctg ggtgcgggaa aagtaccttg gctactacga
    28021 ttatgggatt acttgatcct aataacacaa aaattacagg tgagatttta tataacgatc
    28081 tgaatttaat cgacctgaac gaaacgctct ataataaaat ccgcggaaat gatatcggaa
    28141 tgattttcca agatccgttg tctgcattaa atcctctcat gagaatcgaa gaccagatca
    28201 aagaaggact ttcctatcac acaaagatga atgcagaaca gcgtcaagct cgtgcactag
    28261 aattgctaga acaagtaggg attcctaatc ctgcgagagt tggacgccaa tatccccatg
    28321 aactatcagg cggtatgaga caacgtgtca tcatcgcaat cgcaatcgcc tgcaaacctc
    28381 caatcgtcat tgcagatgaa ccgacgacag ccctagatgt aaccatccaa gcacagattc
    28441 ttgatttact gaaagacctt caggaagaaa caaaaacagg gatcatcttg atcactcatg
    28501 acttaggagt cgtagcagaa atggctgatc gagtggcagt gatgtatggc ggacagttcg
    28561 ttgaagtagc aggtgtaaaa gaactgtttg atcatcctaa gcatccttat acacgctctt
    28621 tactgaactc tataccacaa gaaggaaatc atgatgcaga actccatgtg atcgaaggtg
    28681 tcgttccttc tctgaaaaac atgcctcgta aaggttgtcg gtttgcacca cgtattccat
    28741 ggataccagc aagcgatcat gaagaagagc ctatccttca tgaagtcgca ccgaaccact
    28801 tggtacgttg ctcctgctat aagcatttcc actttagaga cgaaaaagga gaggtgtaga
    28861 tgacagaatt aatcacaatc aaagacctga aagttcatta ccctattcgc agtggattct
    28921 tcaatcgagt aacggatcat gtatatgctg tagatggtgt ggattttatt atcgaaaaag
    28981 gaaagacata tggtttagtg ggagagtctg gctctggaaa atctactacg ggaaaagccg
    29041 ttgtgggatt agaaaaggta acagccggac aaatcatgta tgaaggaaaa gatgtaacga
    29101 agcgaagcaa ccgcaaaaaa atgggttata acaaggatgt gcagatgatc ttccaagatt
    29161 caatgtccag tttgaatcct aaaaagcgag tgttggatat cattgctgaa cccatccgaa
    29221 attttgaacg gttaagcgat caagaagaaa agaaaaaggt aaaaggactg ctagatatcg
    29281 ttgggatgcc ggaagatgcg ctatataaat atcctcatga attttcaggc ggacaacgtc
    29341 aacgtctagg agtagcccgt gctgttgcta ccaatccaaa actgatcgta gccgatgaac
    29401 ctgtatctgc tctggatcta tctgttcaag cgcaagtatt gaatttcatg aaacgaatcc
    29461 agcaagaata cggcctcagc tatttattca tttcacatga tttaggtgta gtaaaacaca
    29521 tgtgcgacaa tatcgcaatt atgtacaaag gtcgatttgt agaaatcggt acaagagaag
    29581 acatttataa tgacccgcga catatctata cgaaacgtct gctttcagct attccgcaaa
    29641 tcgatgtaga aaatagggaa agccacaaag aaaatcgacg aagagtagaa caagagtata
    29701 tccaaaacca aaaagaatac tatgatgcga ctggtcgagt ctacgatttg cgtacgatca
    29761 caactacaca caaagtggcg ttgaaagatg gaggtgcaag ctaatgtgga aaacgattct
    29821 gcgacgtgtt ttattgatga tcccgcaagt tattatttta agtgttctga tcttcttatt
    29881 agcccaagcg atgccggggg atccatttac cggtttgatc aatccaaatc aggatccggc
    29941 agtgatcgaa cagatgcgac aagcagctgg cttgaatgac ccatggtatg tccagtactt
    30001 ccgttggatc ggaaatgctt tgcaaggcga ttttggtcaa agtttcttat acaaacgctc
    30061 tgttgctgac ttgatcggcg aacggatcat caacacttta tatctatcga tcttgacagt
    30121 tgtccttact tatttgattg ctgtgcctct ggggatgctt gctggccggt atcaaaattc
    30181 aatcttggat aagattgtcg taatctacaa cttctttagt tttgctgttc ctttatttat
    30241 cttcggcttg atcatgctat ttgtttttgg ttatcgttta ggctggttcc caacaagtgg
    30301 atcacaaaca ctaggtttgc aaggtggatt tatcactcaa tggatagacc gtttgaaata
    30361 tattctgtta ccatcaatta cccaagcatt tttggcgact gccgtcacaa tccaatatct
    30421 gcgaagtgaa gtaatcgatt cgaaatcgct cgattttgtc agaacagccc gttccaaagg
    30481 ggtaccgacg aataaagtat tcagtcgaca tattttccga aatgcagcgt tgccgatggc
    30541 ttctcaaatg gggtatgaaa tcacttcgtt gattgctggt tctgtagtta tcgaaaagat
    30601 ttttggttat ccgggagtcg gaaaactatt tattgattcc atcggccaac gggactatac
    30661 cgtgatcaca tccttagtat tgattttagg aatcgcgacg ctagtgggga cattactttc
    30721 agatatcatc atgagtatcg ttgacccgcg tattcggatt caataaaacc aatcagaaag
    30781 gagaatggat cgatgagtga aaaaaacaat acagcagttc aagaaagtat tcctcctatg
    30841 gggatacgaa tgattgctcg agaatttaaa aaagataaaa ttgctatttt ttcattaggt
    30901 ttgctagtgg ttttattatt agtgatcttt atcggtgcga tgctgaccga tcaagacaaa
    30961 gtgatgtatg ttagtatctt cgacaaatac gctgcaccag gaacagtttc gacacaagga
    31021 caaaagttca ttctgggagc agatgaaggc ggacgtgatg tattaggaca attgatcata
    31081 ggtgctagaa actctgttac gatcggattt gcgatcacga tccttacttc gatcatcggg
    31141 gttggattag ggatcatctc tggctactac ggagggatca ttgacaacat cttgatgcgg
    31201 atcgttgatt ttatcatgat tctaccgatc atgctgatca ttatcgtctt cgtatcgatt
    31261 atttccagat acaatgtctg gtcctttata ttcatcatga gtgcatttta ttgggtagct
    31321 aaagcccgat tattccgaag caagacatta tctgaagcaa gaagagatta tgtcagtgcc
    31381 tcaaaaacaa tgggaaccaa tgattttaaa atcatgttcc gcgaaatcat gccgaatcta
    31441 agttcattaa tcattactaa tttgacgatg aactttgcgg caaacatcgg tatcgagaca
    31501 actttgactt tcttaggttt cggcttgccg gcaaatgtac ctagtttagg tacattgatc
    31561 ggttatgcaa gtaacggaga agtcttagtg aatcggcaat ggatctggtt acccgcatca
    31621 atccttattt tagttttgat gttgagtatc aattatgttg gacaagcatt caagcgttct
    31681 gcagatgcgc gccaacggtt aggctagtat agaagcgaag ggggaaatag gaatgaagaa
    31741 aacgattttt ggatttgcag cgctgatgtc tgctgtcgtt cttgcagcat gcgggggcgg
    31801 aggtacagat acctctggaa acaactctgg aggcaacggc aataacaaag aggaattaac
    31861 ttttccaatt gcaacaaaaa ataatgatga tgcaatcgaa gacggcgaat tagaagctgc
    31921 ggtcgcaacg gacagtcagt ttaaaggatt gttccagtgg gagttttacc aagattcata
    31981 tgatgcagct tttatggctc cttctcatga accgctattt gcaaacgatg acgatttcag
    32041 aatcacagac gacggagctg caacgcttga tctagatgaa gatacgaaaa aagcgacaat
    32101 caaagtaaaa gacaacgtca aatggtcaga tggtgaagat gtaacggcgg acgatgtgat
    32161 tttcccttac gaaatcattg ggaataaaga ttacactggt atccgttatg acgacacttt
    32221 ccgtaacatc gtaggtatgg aagaatatca tgatggaagt gccgacacaa tctccggaat
    32281 caaaaaaata gacgacaaaa cagttgaaat cgaatataaa gaagtaaacc caagcatgct
    32341 tcaatcgggt ggtggtatct ggtcatatgc agcaccaaaa catacgttag aaggtgtagc
    32401 aatcaaagat atggaatcaa gcgatcaagt acgtaaaaat ccagtgactt ttggccctta
    32461 ttacatgagt aatatcgttg caggagaatc agtagaatac ttgccaaacg agcactactg
    32521 gaatggtacg cctaaattga aaaagatcac gatgaaatct gtaccgatgg caagtgcaac
    32581 tgaagcattg aactctaaat tatatgatat gattttccaa atgccaacag atacttacga
    32641 tacctataaa gaaattgctg gctatacaaa cttaggccgt gaacaaactt cttatactta
    32701 tctaggattc aagttaggta aatggaatgc agatacaaag agtgtggaat acaatccaga
    32761 ttcaaaaatg gctgataaat cattacgtca agcaatgggc tatgcattag ataataatgc
    32821 agtaggtgaa cgtttctatg caggtttgag aagcaatgcg acttcattga ttcctccagt
    32881 attccagtca taccatgatg atgagatgaa aggattcaca caagatctag acaaagcgaa
    32941 aaaactatta gatgatgctg gctacaaaga tgtggatgac gatggtttaa gagaagacaa
    33001 agatggaaat aaactaacaa tcaactttgc ttcgatgtcc ggtggggaaa cagcgcaacc
    33061 attggcagat tactatatcc aacaatggaa agaaattggt ttggatgtcc aatacacaac
    33121 aggtcgtctg atcgaattca attctttcta tgataaatta gaaaacgatg atcctgaaat
    33181 tgatgtttac caagctgctt ggaatacagg aactgatcca aatccaagtg gactatgggg
    33241 accaaatgct gcgttcaact acactcgtta tgaatcagaa gaaaatacaa aattgatcaa
    33301 cgatatcagt tcaagcaaag catttgatga agagtaccaa aaagaagcat atcaaaaatg
    33361 gcaacaatat gcatttgaag aagcatttgc gattccaacg ttgttccgta atgaagtatt
    33421 gccagtcaac gaccgtgtca aagactggga ttggtcttat gatgtaccaa acccatgggc
    33481 aaatgtttct gttacagcag attcacgttc ataatcacta aaaatgaccg aaaaagggcg
    33541 atgtcctttt tcggtttttt gatatttttc tgtttatttc tctaatattc aggtcaaggc
    33601 gctgatttca tgacttctcc aataaacggt gttcaaagtc cagtttctcg gtattaagtc
    33661 aaatttatgc aaaaatatgg aaagccattt tcacaaaatt ttttcttcat actcgaaact
    33721 aaacgaattt ttcacaactt ctcgtcaatc actaggatta ttaaagcttt acttaccttt
    33781 tgaccgaacc atttcaaatt tgacaaaaaa ccagtataat aaggaagtat cgaaaaaata
    33841 tccataaaag gaatgtggaa aatggacaac cagctgacaa aagaactaaa agaaaactat
    33901 aacatcgttt ttcacgatct aaatttgctt gaacaagctt ttacccattc atcctatgtg
    33961 aatgagcatc gcaatctgca attatcggat aatgagcgac ttgaattttt aggagatgcc
    34021 gttttagaac tgatggtttc tcagtattta ttccgtttat atcctcattt gccagaagga
    34081 aaattgacaa aaacacgagc tgcgatcgtt cgcgaagaca gcttgtcgaa atttgccaaa
    34141 gaatgtcact ttgatcaata tgttttgttg ggcaagggag aagagaattc aggaggacgt
    34201 acgcgaccag cattgctttg cgacctattc gaagcttttt taggcgcgtt atatcttgac
    34261 cagggattcg acacgaccca tgcattcatc gaaaaagtga tttttcctaa agtcaaagca
    34321 ggtgcttttt cacatgagat ggatcataaa acaaaactac aagaagtctt gcaaaaatca
    34381 ggtgatgtat caatcgaata ccgattgatc aatgaagaag gtccagctca tgaacgagta
    34441 ttttggatcg aagtttatgt agatgatcaa ttgattggca ctggacaagg gaaatcgaaa
    34501 aaattagcag aacaagctgc ggcggaaaat gcattggcag cactgtaaaa gtaagtgagg
    34561 gaaagctttg tatttgaaac gaattgaaat agccggtttt aaatcatttg cagacaagac
    34621 agtcattgat tttgaaaata gcgtcacagc agtggttgga ccaaatggca gcggaaaaag
    34681 caatattaca gaggctatcc gctgggtatt aggagaacaa tctgctaaaa gtctgcgagg
    34741 cggaaaaatg ccggatatca tctttgccgg ttccgatacc cgtaagcagc tgaatattgc
    34801 agaagtcaca gtgattttag acaatagtga tcattatctg ccattggagt acaatgagat
    34861 cagtgtaacc cgccgttatc gacggactgg ggaaagcgag tttttcatca ataaacaaag
    34921 ctgcagatta aaagatatcc aagaattgtt cttagattct ggattaggaa aagaatcgtt
    34981 ctcaatcatt tctcaaggaa aagtagaagc gattttcagt agtaaaccgg aagaccgtcg
    35041 tgggatcttt gaagaagcag ctggtgtctt gaaatacaag cagcggaaaa agaaagcaga
    35101 acagaagctt tttgaaacag aagacaacct cagccgcgtc caagacatca tccacgaatt
    35161 ggaagaacag ttaactcctt tggccgccca gagtgaggca gcaaaggagt ttttacggct
    35221 aaaagaaaca ctgactcaaa ccgatgtctc tttgatggtt gctgaaatca agacagcaaa
    35281 aaaagattgg gacaacaaac aagcacagct tgcgaaattc aatctagaat taggcaaact
    35341 atctgagtcg atccaagaac aagaatccat tttggcgaag cagcgtaaag aaaatgcgca
    35401 agccgatcgt ctgatcgaaa aaaatcagca agtattatta gatctttcgg aaaaactgaa
    35461 gcagacagaa ggacaaaaag atgttttgca ggagcggaca aagcatacac aaaaaagctc
    35521 gcaagaatat caaacttcat tagcagaagc acaaaaaaag gttaaacatt ttgaaaagct
    35581 tcaagaaagc ttgatgaaag cagcagcaga aaaagaaaca gagatccaaa aagctgaagc
    35641 aaatctcata aaaacacagc aagaattaga aaaataccaa aaatcaacga aagaattatt
    35701 ggcagaatta agagatcaat atgttgattt gatgcaagaa caagcagctg taggaaacga
    35761 actgaagtat ctagaacgtc aatatataca agaaacagca aaaagcaagc agacgttagc
    35821 caagcaatcg gaagtagaag catcagtaga ccgattgatc ttacaaaaag aagaattgac
    35881 acaaaagcag gcgcagctaa aatcatcttt aactgaaaca aaagagaagt tagaaatgat
    35941 ccaacaaaac gggaaaaagt tccaagaagc attagcaaaa gaacagccta aaatgtatca
    36001 attaatgaat caagtccagc agctacgtgc tcgtaagaaa agccttcaag aaatccaaga
    36061 aaactatttt ggtttttacc aaggggttcg attggtcttg cagcataaac agcagctttc
    36121 gggtattgtc ggagcagtgg ctgaactgat cgatgttcct gcagatttca cgttagctat
    36181 cgaagcagct ttaggtgggg cagcccagca tgtcatcgtt gaaaatgaaa aagatgctcg
    36241 gcaagccatc acgtatttga aacagcaaag aggaggaaga gcaacattcc ttccactgac
    36301 gacaatcaaa ccaagacaat taccggctca tatattgaca caagcagcag ctgtagaagg
    36361 gtttataggg attgccagtg aacaagtcag ttatcccgat caaatccaaa ctgtggttca
    36421 aaacctgcta ggaacgatcc ttttagccaa agacttaact agtgccaatg caatcgcgca
    36481 aacgatccgc tatcaatatc gagtggtttc cttagaaggt gatgtgatga atgccggagg
    36541 atccatgaca ggtggagcga ataaacgagg aaaccaaggc agtttgtttg tacaaaatca
    36601 agaattgaaa cagttgacta gtgaatttga agaagcagat aaacaactcc aagcacaaga
    36661 aaaaaaagtt caggagctgc aacaagaaac agctcgttta gccgaagaac aagaagtttt
    36721 gcgtacacga ggcgaacaat tacgttttga agaacaagaa gcgacaaatc agctacaaaa
    36781 catcataaat gaacttgaac gttttgaaaa agaaaagcaa atctcaactt ttgagacacg
    36841 tgaactgcag cagtttatcg aagattacga aaagcaaacg aacgaattga aagacaaaca
    36901 aacagatctt gaatcgcaac gacaaaaaat cgacgaagaa atcaaatcgt taagtcaaga
    36961 aagcgaccag atggaagcca gaagagcaca agtacaaagt caaaaagcac aagaacaagc
    37021 agatcttgct gtcttgaaag agcagtttaa tcatctgcag atccagctcc gcggtgcccg
    37081 agtccaaaaa gcagaggcaa ccgaacgtca agaagcgata gaaaagcagt tggcgacatt
    37141 gacagcagac ttttctgatc atgaagtcac agaagaaagt ttggaaaaac aaattaacga
    37201 attatctgct cagcgagaaa cattaaaagc cgagttggca aaagccaaag agcagcgaga
    37261 tagaaggcaa aaggagatcg ataagcttga agcagtcttg gcagagcgta atcgtgagca
    37321 aaaagcacga ctgtcggaac aatccaaatt agaagtccaa aaagaccgag ctgaaatgat
    37381 tttagataat catttgacct atctgcagtc agaatatcag ctgacatttg agaaagcttc
    37441 ccaagactat caagaaacaa ccgatatcga agacagtaga acaaaggttt catccttaaa
    37501 agaacagatt gaaaaactgg gaccagtaaa cttaaattca atcgaacaat acgaacaagt
    37561 cagtgaaaga catacattct tagcaacaca gcgagatgat ctgttagcag cgaaaaacca
    37621 attgtttgaa acgatggatg agatggatga tgaagtgcgg acaagattca aagaagtatt
    37681 tgaagctatc cgtcaagaat tcaaaattgt ctttccaaat atgtttggcg gcggtaaagc
    37741 tgaattagta ctgacggaac caactgattt gctgaaaaca ggtattgaga ttgaagtaca
    37801 acctcctggc aaaaaactgc aaagtctaag tttgctttct ggaggagaac gagcacttac
    37861 agccatcgcg ttgctctttt ccattatccg tgtctgtcct gttccgtttt gtatcttaga
    37921 tgaggtagaa gcagcgttag atgaagccaa cgtgaaacgt tttggtcgtt acttgagcga
    37981 tttccaagat gacacacaat ttatcgtagt cacacaccgc aaaggaacga tggtcgcagc
    38041 agatgtactg tacggagtga cgatgcagga atccggtgta tcaaaaatcg tttctgtacg
    38101 gatggaagat atcaatgaag aggcgaaaat caacgaaacg aaagtgtagg aacgaaaatg
    38161 atcaaattag tagcaatcga tttggacggt acattattaa atgaacaaaa gctgatttca
    38221 gatgagaata aacaagcatt agcacaagca aaacagcaag gggtcaagat cgttctatgt
    38281 acaggacgcc ccttagctgc gatggcgcat tatctgcaag aattaggact ggtagatgaa
    38341 ggtgactaca gcatcacttt caacggcggt ttggtacaaa aaaatgatac tggtgaaatc
    38401 atggaaaaga aagtgatgga agtatcagat atccaacgct tatacaaact ggctcagcaa
    38461 ttggatctgc cactagatgt tttgtctgac aatgtggttt tacagctgcc tagtgcccca
    38521 aggaagcaat ccttgtacaa tgtgttgaat aagctgctgc aattccaacc agcggatata
    38581 agcgatatta cagatgaatt tgtgcttaat aaggcagtaa tcgcttatga ccaagaagaa
    38641 ttagatccta aaatcaaaga aataccagca gaattccatg aaatgtatga gatcatcaaa
    38701 acaagaagta tgcttttgga atttatgcca aaaggtgtta caaaagctta tggtatttct
    38761 ttattggcaa aagatcttgg tttgaaacaa gaagaaatca tggcgatcgg tgatgaggaa
    38821 aatgatttac cgatgatcca atatgctgga ctaggcgtag cgatggagaa tgctgtgcct
    38881 ttcgtcaaag aagcagcaga tttcgtgaca gccagcaatg aagaaaatgg cgtagccaaa
    38941 gtgatccaaa aatttgtatt agagtagggg gagtcaaatg ggattttttg ataaaatcaa
    39001 gcgtgctttt actggcgaag agaaaacaga agaagttgaa gtacaggaga agtacgacaa
    39061 gggtttagaa aagacgcgta aaacatttgg tgaacggatg aatgagttgt ttgccaattt
    39121 tcgaacagtc gatgaagact tttttgaaga attagaagaa acacttatcg gtgcagatgt
    39181 aggttttgaa acagcgatac gaatcaccga agcattacgt caagaagtga aactcagaaa
    39241 tgcgaaaaaa tcggcagaag tccaaaatgc gatcatcgag aaacttgtgg atctctatga
    39301 agcggaagga atcaatgaag taaatgaact aaatatccaa gaagacggac taacagttat
    39361 tttgtttgtc ggtgttaatg gtgtagggaa aacaacaagt attggtaaac ttgcccatca
    39421 gttcaaaaac gaaggtaaaa aagtattgat ggcagcagca gataccttcc gcgctggagc
    39481 aatcgaccag ttagttgtct ggggcgaacg cgctggtgtt gaagttgttc gtggcaatgc
    39541 aggaggcgat ccagcagctg tcgtattcga tgctttggaa agagcaaaga cagagcaggc
    39601 agatgtttta ctagttgata ctgcaggccg actgcaaaat aaagtgaact tgatgaacga
    39661 attagaaaaa ataaaacgag taatccagcg ggaattacca gaagcacccc aagaaacact
    39721 tcttgtatta gatgcaacca ctggtcaaaa cgcgatggtt caagcaaaac aattcaaaga
    39781 aacaacagat gtgaccggtc ttgttttgac aaaattagat ggtacagcaa agggcggtat
    39841 cgtacttgct atccgtaatg aattgcattt accagtgaaa ttggtcggtc ttggtgaagg
    39901 aatcgatgac ttggagccgt ttgatccaaa tgattttgtc gttggattgt tcaaaggatt
    39961 actaaaagaa gaataaccca tgtctaaaaa gcaggagctg tgacaaaagt tgcagctcct
    40021 tgtttgaatg attgttttta acatagctgt gtttttgatg ccttcatttt tatacttatt
    40081 tgtaagcggt tctcaaacaa tttcgttctt tttcttagac tttttgatta taattagatt
    40141 catatgaatt ggaggtcact taatgatgaa ttttaagcaa ttattattgc atgtcaatgc
    40201 gcgccctttt atcgaccaag ctcgatttgg tatagaacgg gaaggacagc gggttgatct
    40261 tgcaggaaat ctagcaaaaa ccgatcatcc agcaatcttt ggcgatcgat cctatcaccc
    40321 ctatatccaa acagatttta gcgaaacaca aacagagatg atcacccctg ttaccgattc
    40381 tattcccgaa ttatttcagt atctcgctgc tgtttatgat gtgactgctc gttctatacc
    40441 gaaagaagag atgatctggc cattaagtat gccacctgcc ttaccggaaa aagacgaaga
    40501 gatcattatt gcaaaattaa aaaatttcga agatgtcttg tatcgacgtt atttagcaaa
    40561 agaatacggg aagcgaaaac agatggtaag cggtattcat ttcaattttg aatttggcga
    40621 tgagttgcta agaacattgt tcagccatca ggaagaattt caagattttt ccgaattcaa
    40681 aacagaactt tatttgaaaa cagcgagaaa ctttatgcgc tatcgctgga tgatcactta
    40741 tttattcggt gcttcaccaa tgagtgagaa gaactacttt ttagatgaat cacatccgca
    40801 agaacctgtt cgcagtattc gcaacagtgc gctgggctat acgaatcatc caaatgtgaa
    40861 agtttcctat gcttctatga aacagtattt agcagatatc gagcgaatga tcgaagaagg
    40921 gaaactttcg gaggagaaag aattttatac accgcttcgt ttccgaggcg gaaagaaagt
    40981 cgcagattta gcaacaacag gtgttcgtta tattgaattg cggaatatcg atttgaatcc
    41041 ttatgcaaga ttgggtatca atccagagca agttcggttc ttacaattat tcctgatgta
    41101 catgttatgg acagaagaaa aagaagactg tgaccaatgg gtagcagaag gtacgactcg
    41161 aaataacaaa gtagcactag aacaaccatc agatcaaaca gaatttcatc aagaaggtag
    41221 agaaattctt gaaggaatga agcaaatgct ggttgaattg gattggttgg attctcttta
    41281 tttagtagag gaagcattga ctcaaatgga tcatcctgaa caaacgttag cagcgaaact
    41341 ctatcaagaa gcacagctat cgagtcagca agaagttgct gtggcattgg gacatcaata
    41401 ttataaagaa agccatgaac gtccctatca gttggccggt tttcgcgaaa tggaactttc
    41461 tactcagata tttatgttcg atgccatcca aaaaggtgtc caagtcaaag tattagatga
    41521 gtcggatcaa tttttgcgtc tgcaattcca agaccatgta gaatatgtga aaaatgcaaa
    41581 catgacaagt aaggacagct atatcgtccc acttatcatg gaaaacaaaa cagtaacgaa
    41641 aaaagtctta aaagaagctg ggttccgggt accaggaggc gcagaatttt catccatgga
    41701 agaagcggta aaagcttatc cgagatttgc cgagcaggca tttgtgatca aacctaaatc
    41761 aaccaattac gggttaggta tcacgatttt caaggaagga gctagtctgg aagattatca
    41821 agcaggttta gccattgctt ttcgtgaaga cagttcggtt ttagtagaag aatttatgcc
    41881 gggaacggaa tatcgtttct ttgtgatcga cggagaagtc caagccatca tgttgcgagt
    41941 ccctgctaat gttattggtg acagtatccg aacagtgaaa gaacttgttg aagaaaaaaa
    42001 tagtgaccct ttgcgtggaa cgaatcatcg tgcaccttta gaattgatcc aattaggtga
    42061 attagaacaa ttgatgttga aagaacaagg gttgacgatc gaatcagttc cccaagccaa
    42121 tcaaattgtt tatctaaggg agaactcaaa tatcagtaca ggtggtgatt cgatcgatat
    42181 gactgatgaa ttttctgaag cttacaaaaa aatcgccgtt tctgcggtag aagcactagg
    42241 agctaagatt agtggtatcg atttgattat accagataaa gaaatcgatc ctacaactga
    42301 taaaaaagca tatgggatca tcgaagcaaa tttcaatcca gccatgcata tgcatgttta
    42361 tccttttgca ggaaaaggca gaagattgac gatgaatgta ttgaaattgt tatatcctga
    42421 agtattttaa aagaaaaagc ttttgacaga ttgtcctatt tgatgtcaat ctgatcaaaa
    42481 gctttttttg catgtaaaaa tttatgtgtt ttttcgtttt aaaataaacg atcccagaag
    42541 ctttttttct tttttggttt ttctttttca gctggcgaag agttttcttc tgggtatttt
    42601 ttttcgatat caatcacagg ttcatttact gcttcttttg ctgttaagag caatccgaat
    42661 tcctcaggat ttgcagtgac gtaatcgttg actacagtga aacggctatt gtttttcgtt
    42721 gctagctgga tatattggtt ttggacagaa aggggtagag ccccattcaa gagtatggaa
    42781 gcatctggat gttttttgag ttcttttaaa aagttttctt tgttttttgg ttggatcatt
    42841 tcagcaacag tcatactaag atagcagcgt tccctgaatg tccctaaata tttatgctgt
    42901 tcttcagggt tcactaaagg tgtaccataa agtcccttat ccaaatggct ttttacatca
    42961 tcttgtgcca taaaaaatac cctccttctt ctctttgtct ttcttacgat tatagcatat
    43021 tcccaataat acctaaaaca tatgaaaaag cggttgcttt tgttcaacga ttcacttaaa
    43081 ataagaatga aaatcattct caattagaaa ggtggattgg atgcgctacg gacgactagt
    43141 agcattatta gttattttaa gtagtatttc attgttcatc ggagtatcga atgtacaatt
    43201 gtcagaactg ctccagttag atgggcaaag cttacaaatc ttgctagtga ctcgtttgcc
    43261 acgaacgatt tgtttgatcc ttgtgggagc gaccagtagt atttgtgggt tgatcatgca
    43321 acatttgacc caaaataaat ttgtttcacc aacgactgcg ggcacgatgg atagtgcgcg
    43381 tttaggtatt ttagtggcta tgattttcct acctggtgca tccttgattg tccgatcctt
    43441 gacagcattt tgctttgctt ttgcaggaac attgcttttt ttatccctta cgcgattgtt
    43501 cctccagaaa aatcaattga tgcttccgtt gattggagtc atgttaggga atatcattgg
    43561 ttcagttgct actttttttg cttatcaatt ccagttggta caaaatatga cttcatggct
    43621 tcaagggaat tttgcgactg tgatacgagg aaattatgag ttattgtatg ctgttgttcc
    43681 attgttgctc ttggtttggc tatttgctta ccgatttacg atcattggtc tgggcgaatc
    43741 gtttgcagta aatctgggaa tagattacca gaagatgcag tttttaggca ttggcttagt
    43801 atcattggct agtgcagtca tgttgatcat ggtaggaagc attcctttta tcggagtgat
    43861 cattccgaat cttgtctcta gaatatatgg agatcaagtg cacaaaacaa ttggcgtaac
    43921 tgctttagca ggtagtttgt tcttagtatt ttgcgatctt gtcgctcgcg tgttgatttt
    43981 tccttatgaa ataccagtca gtgtgatcgt tggaatcatc ggtggggcta tcttccttta
    44041 tcttctgata aggggacgac gatatgctta aaaaaaagcc atctattttt attggtatgc
    44101 ttgttcttgt tttatttgtt acaggaatgt acttgacgat ccatacatat ggaaactggc
    44161 attttgcatg tgcattgagg agtaagaaaa tttttgcatt tatccttgtc tcgctagctg
    44221 tcagcagtgc cacaatcagc tttcagacaa ttacgcataa ccagtttttg actccttcag
    44281 tattgggact tgagtcgtta tatgtccttg tccaaacggt ccttttcttc tttatcggag
    44341 gagtgacgat gcttcagcag acaggtacag tttttttctt attgaacgtt gggctgatgg
    44401 tcgggctgag tctattgtta tttttctttc ttttgaaaaa aatgagagcg aatctttttt
    44461 tattactgat gtcagggatg attttaggta cgctttttgg tagtgtcagt acctttttac
    44521 aagtcatcat ggatccgaat gaatatgatt tgcttcaagg cagattattt gcaagctttg
    44581 gcaatatcca gagtcagtat cttttgcctg cttctttgat cattatcggc tgctggtacc
    44641 tgttatttcg atatcgacat gtgctagacg ttttccatct cggaaatgaa caagcagtaa
    44701 acctcgggat agacacagat cgttttgaat ttttgatttt atgttgtgtc agtcttttga
    44761 cagggacatc aacggctctt gtgggtccag ttactttttt agggtttatt gtcgcaaata
    44821 tcagttatcg tgtcttttct acttatcgtc atggtcagct atttatcgga agctttctgg
    44881 tagctatgct gtttctgaca ggaggacagc tcgcagtaga gcagattttc ggctggaata
    44941 caacagtgag tgtcgtcgtt gaatttgtcg gaggaattta ttttgtcaca aaattgattc
    45001 gcgaaaggaa gagtatccta tgattgattt acaaaaggtc aataaactgt accatggaaa
    45061 atcagtggtt aaagatgtac agttaacgat tcctgaaaag aagttgactg cttttatcgg
    45121 tccaaatggt gctggaaagt caacggtgtt gtcgatgatc agtcgtttga taccaaaaga
    45181 tacaggtgaa atctatttag accataacga agtaaaggct tggaaatcca atgaattagc
    45241 caaaaaatta tcgatcctac gacaatcgaa tgaaataaat gtccaaatca ccgtgaaaga
    45301 attagtagaa tttggacgat ttccttatac gaaaggaaaa ttgacgacaa aagacgagga
    45361 aatcgttcag gaatcactgg aacacattgg gttagaagaa atagcggatc aatcgattga
    45421 cactctttca ggcggtcaac tccaacgtgc gtatattgca atggtgctag cgcaagacac
    45481 ggattatatt ttactggatg aacctttgaa caatctagat atgaattttg cggttcaaat
    45541 catgcagatt ttaaaaaatt tagtacaaga aatcgggaaa acaattgtta ttgttcttca
    45601 tgatatcaac tttgctgcaa gttatgcaga cgaaattgtt gcaatgaaag atggacagtt
    45661 atttacgcaa ggaacgaccc aagaaatgat aaaaaaagat gtattggatg aattatacga
    45721 aatggacata cggatttgtg agattgaagg ccgcagattc tgtctttatt tctaaaacta
    45781 acattaggag agaagagatg aaaaaattag tagtaggtat cttgattcta gcatcatttg
    45841 gtttagcagc atgtggaaat agcaacaaca ccagtcaggc agacacaaaa aaaagcagca
    45901 cacaaactga aactacattg acgataacag atagtaatgg agaccaaatc gaggtgccaa
    45961 ataatccaga aaaagtcgtc gtattcgaca atggttcttt agatacgatg gatgctcttg
    46021 gtgttggaga taaggtagtt ggagcagcta caagcagcct tcctgagtat ctttcatcct
    46081 ataaaaaagt agaatcagca ggtggaataa aagaaccaga cttagaaaaa attaatcagc
    46141 ttcagccaga tctaatcatc atttctggcc gacaaagaga ttttcaaaaa gatctaagtc
    46201 aaattgctcc gaccattttc ctttctttag atgcgaaaaa tccatgggaa tctttccagc
    46261 aaaatgtcac tgctttaggg gagatctttg gtaagcaaga agaagctaaa actcaattag
    46321 aggaattatc tagtgcaatc gatcagacga aaaagaaagc agaagctact gacaaaaaag
    46381 cactagtcac acttgtgaat gaagggcaat tatcagccta cggatctggt tctagattcg
    46441 gtttcatcca tgatttattt ggctttgaac aagcagatga tcaaatcgaa gcatccactc
    46501 acggtcaaag tgtttcttat gaatatgtgc tggaaaaaaa tccggatatc ttatttgtag
    46561 tagacagaac aaaagctatt ggtggagacg acagcaaaga tgatatttct gccaatgaac
    46621 tggtagcaca aacgaatgct ggtaaaaatc agcagatcat ctcccttgaa ccagatgtct
    46681 ggtatttaag cgggggcggt ttagaatcaa tgaaactgat gatcgaagat gtgaatcaag
    46741 catttaaata gcataaataa cagaggatta aactctcata ctatgtaaca ataatgtttc
    46801 ttacattctt aaatagcaag agcctatcct caaagaagtt atcaaaaacc atccgtaaaa
    46861 tcgtattgat gattttacgg atggttttta tttatttgag aaatcttttt tcaacgtgaa
    46921 ttcattatct acatcatacg ggttgattct agaatgggga gtggcaaaaa atagtgtgat
    46981 ccattttttc agcatcaaga tcgcctcctt tgatttgaat caaaggtaca aagcgaaact
    47041 tagataaaac ttaaatttag atatttttat tttgtaccga tctcttcttt aaacgatgtt
    47101 ccatctgctg cttctcggca ataagtccac tagactagaa aatatgagaa gctattttct
    47161 gtccagcgtt cttattactc aaaacaaatc gcagttgtca caatctcttc taaacgatgt
    47221 tccatctgct gcttctcggc aataagccca ctagactaga aaatatgaga agctattttc
    47281 tgtccagcgt tcttattact caaagcaaat cgcagttgtc acaatctctt ctaaacaatg
    47341 ttcaatcagc tgaccttcgg tattagctca aagcactgat ttcacaacct ctatttaggc
    47401 atgtgtctca gcaagcggtt gatagttgat gatctgcatt tttcgataaa aatcggcggg
    47461 aagcttactc caccgtttta gtaattgata gaaataactc tcttctgctt tgattttata
    47521 ctcgcttaat atcaatccaa aactattcgt tttttttgaa gctaattttt tataaatatc
    47581 caatgctttg gttttatctt cattgacaaa gagtgttttt acttcaatat aagaaccctc
    47641 gatattttgt gtttcttcga taaaatagat gttttttata tattccttca tagtgattcc
    47701 tccaaagaag tcttttattt gataagttta ctctaccgaa gagttgttaa taatccgtga
    47761 acaaaaagta attctttttc caatagttgt taaaggtttt ttatttttta tagtattttt
    47821 tgttatttaa gaaaggaata aggttaaaca aataacaggt tgctttttgg gtctttttcc
    47881 gttcaagaaa ctgaacgaag aaaagggaaa gttgtctctt ttgtgataag agaaatcata
    47941 ataaacgaat gacttacttt ccaataaatt gagaacagtc agaaccagac aaattagttg
    48001 catctgatga ataaagtgat tattctatga catgaggtga tacagatgaa attagaagtg
    48061 acaaaagaag cacaagaagt attgaagaat aaattagaag cagacgatca acttttatta
    48121 gatatcgaaa acggtgacgg tccatttgca gaaagccaag tcagttgcca attggatacg
    48181 gcatttcgat tgatcattgt caaaaaagat acacaaacag atctaagtct ttattctgta
    48241 gtggctgata caccagttgg accaatcaaa atcaaagaca gtgcaatact ttacatggat
    48301 gatccatcca cacttgcttt agaaccaact tataatagtt tacaattaaa aggaccaagc
    48361 ggcttgagaa aaggcaactt gcaagttgtt cgaaaagact agagggataa aatgggaaaa
    48421 ttggcgatca tcagcgacct acatgtggac atcaataaat tcggcgaatc agagctgatg
    48481 cagctttggc aagttttgca aaagcacaag atcactcggc ttcatctagc cggagatacg
    48541 gctaataaag tagaccgttg cctagctgtt gtcgaatttt tcgtcaataa aggattatct
    48601 acgacatttc atttcggcaa tcacgagttg gcagatgtgc ccaatgaaca gatgatggac
    48661 cattttccag acccacattt tttaaatgaa cggtatattg ctttaaatga gcagaccgtt
    48721 cttttaggga tgaatggctg gtacgactat cagttttccg aattgaaaga ttacgaggca
    48781 atcgtacgaa tgaagcgttt gtattggtat gatcggttca tccagcgtgg gaaaacagat
    48841 ctcgaggtca atgcagaaat gcttgcagct gcaaaaaatt tgctagacca gctggaagca
    48901 aaaaaacttg aggtgatttt atccactcat ttcgttccta aacgggcatt tatcatgtat
    48961 caatctgctc cttatgagcg ttggaacaaa ttaaatgcat ttttaggatc agaatcattc
    49021 ggagaattac ttgaccgtta ttcaaatatc aaacaagtag tttttggcca tacgcatcgt
    49081 cgctttgaag ataaatccat cggtcacacg atttacagct gcagaccttt tggttattat
    49141 tatgaatggc agctgacccg agattttgtc ttgaaagaac agttgctaga cagctatgat
    49201 cctatgaaat tacgtgtcct cttgcgtgcc catcacccag cttttcaggc ctatcaaaca
    49261 caacatttgc aagaggaatt tgaacaagcg atgacaatcg ttccgtattg agacagcatt
    49321 agtaatgtgt aaaagaaaat accttgtaaa a
//
LOCUS       DACDUU010000013        48940 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.013, whole genome shotgun sequence.
ACCESSION   DACDUU010000013 DACDUU010000000
VERSION     DACDUU010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.013"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            591..1280
                     /locus_tag="IWL30_000776"
     CDS             591..1280
                     /locus_tag="IWL30_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9705564.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="IWL30_000777"
     CDS             1267..2475
                     /locus_tag="IWL30_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9705565.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="IWL30_000778"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="IWL30_000778"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAP9705566.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="IWL30_000779"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="IWL30_000779"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAP9705567.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="IWL30_000780"
     CDS             complement(6578..7273)
                     /locus_tag="IWL30_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAP9705568.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="IWL30_000781"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="IWL30_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAP9705569.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="IWL30_000782"
     CDS             complement(8472..8663)
                     /locus_tag="IWL30_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAP9705570.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="IWL30_000783"
     CDS             complement(8684..9574)
                     /locus_tag="IWL30_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP9705571.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="IWL30_000784"
     CDS             complement(9561..10328)
                     /locus_tag="IWL30_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9705572.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="IWL30_000785"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="IWL30_000785"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAP9705573.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="IWL30_000786"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="IWL30_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAP9705574.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="IWL30_000787"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="IWL30_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAP9705575.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14944..15282
                     /locus_tag="IWL30_000788"
     CDS             14944..15282
                     /locus_tag="IWL30_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705576.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="IWL30_000789"
     CDS             complement(15450..16208)
                     /locus_tag="IWL30_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP9705577.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="IWL30_000790"
     CDS             complement(16226..17044)
                     /locus_tag="IWL30_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP9705578.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="IWL30_000791"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="IWL30_000791"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAP9705579.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="IWL30_000792"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="IWL30_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAP9705580.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="IWL30_000793"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="IWL30_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAP9705581.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="IWL30_000794"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="IWL30_000794"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAP9705582.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="IWL30_000795"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="IWL30_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAP9705583.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="IWL30_000796"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="IWL30_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAP9705584.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="IWL30_000797"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="IWL30_000797"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAP9705585.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="IWL30_000798"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="IWL30_000798"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAP9705586.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /locus_tag="IWL30_000799"
     CDS             27086..27385
                     /locus_tag="IWL30_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAP9705587.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="IWL30_000800"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="IWL30_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9705588.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /gene="rpsR"
                     /locus_tag="IWL30_000801"
     CDS             27973..28209
                     /gene="rpsR"
                     /locus_tag="IWL30_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAP9705589.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30325
                     /locus_tag="IWL30_000802"
     CDS             28352..30325
                     /locus_tag="IWL30_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAP9705590.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30331..30783
                     /gene="rplI"
                     /locus_tag="IWL30_000803"
     CDS             30331..30783
                     /gene="rplI"
                     /locus_tag="IWL30_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAP9705591.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31057..32424
                     /gene="dnaB"
                     /locus_tag="IWL30_000804"
     CDS             31057..32424
                     /gene="dnaB"
                     /locus_tag="IWL30_000804"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAP9705592.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33204..34496
                     /locus_tag="IWL30_000805"
     CDS             33204..34496
                     /locus_tag="IWL30_000805"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAP9705593.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            35283..36146
                     /locus_tag="IWL30_000806"
     CDS             35283..36146
                     /locus_tag="IWL30_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9705594.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(36275..37897)
                     /locus_tag="IWL30_000807"
     CDS             complement(36275..37897)
                     /locus_tag="IWL30_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9705595.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            38146..38667
                     /locus_tag="IWL30_000808"
     CDS             38146..38667
                     /locus_tag="IWL30_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705596.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(38853..40583)
                     /gene="spxB"
                     /locus_tag="IWL30_000809"
     CDS             complement(38853..40583)
                     /gene="spxB"
                     /locus_tag="IWL30_000809"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAP9705597.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            40748..41566
                     /locus_tag="IWL30_000810"
     CDS             40748..41566
                     /locus_tag="IWL30_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAP9705598.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(41669..41866)
                     /locus_tag="IWL30_000811"
     CDS             complement(41669..41866)
                     /locus_tag="IWL30_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705599.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            42035..42613
                     /locus_tag="IWL30_000812"
     CDS             42035..42613
                     /locus_tag="IWL30_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP9705600.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(43658..44161)
                     /locus_tag="IWL30_000813"
     CDS             complement(43658..44161)
                     /locus_tag="IWL30_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP9705601.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            44520..45545
                     /locus_tag="IWL30_000814"
     CDS             44520..45545
                     /locus_tag="IWL30_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9705602.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            45868..46746
                     /locus_tag="IWL30_000815"
     CDS             45868..46746
                     /locus_tag="IWL30_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705603.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            47222..47338
                     /locus_tag="IWL30_000816"
     CDS             47222..47338
                     /locus_tag="IWL30_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP9705604.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            48266..48604
                     /locus_tag="IWL30_000817"
     CDS             48266..48604
                     /locus_tag="IWL30_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705605.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            48698..48928
                     /locus_tag="IWL30_000818"
     CDS             48698..48928
                     /locus_tag="IWL30_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705606.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
BASE COUNT        15089 a         9137 c         9590 g        15124 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tgtattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa acctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagat caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgtaaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggatcaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggtggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccggc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagacatt gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atggggaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatcc
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaatcct tgaaaactat
    25441 atcgaacatc aacgcgaagt catcacacga cggacggtct ttgaaaaaga aaaagcagaa
    25501 gcaagagcac acatcttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaggcaaaag caacaatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgtacagc caaaggaatc ccaatcatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggtcagagg gatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aaaatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaataa ccatcaagac taagtgtgta agctagcaag
    26881 aataaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataattag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattagatga
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaat gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atattgaatc ttcagcttct tcagcttcag agctagtttc
    29761 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    29821 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    29881 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    29941 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    30001 tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat
    30061 agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat
    30121 tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt
    30181 ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact
    30241 ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat
    30301 gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa
    30361 ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta
    30421 atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa
    30481 aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa
    30541 ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt
    30601 ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc
    30661 aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac
    30721 gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa
    30781 taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac
    30841 cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag
    30901 attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa
    30961 aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt
    31021 gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga
    31081 atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca
    31141 gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat
    31201 caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt
    31261 actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat
    31321 ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc
    31381 gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa
    31441 ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg
    31501 gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc
    31561 acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact
    31621 ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt
    31681 gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga
    31741 acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta
    31801 aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt
    31861 tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt
    31921 tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta
    31981 gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt
    32041 aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg
    32101 gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag
    32161 cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat
    32221 gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac
    32281 gatgatggaa acgagccaaa aaatgacaac gtcatcgaag tcatcatcga gaagaaccga
    32341 agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg
    32401 ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct
    32461 ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat
    32521 ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc
    32581 aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg
    32641 tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt
    32701 gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt
    32761 ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct ttcttaaacg
    32821 gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgagaagcta
    32881 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    32941 cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa atttgagaag
    33001 caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac aacctctttt
    33061 ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa aataaaaata
    33121 aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt tcaggattaa
    33181 ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg caatggggcg
    33241 atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg attgcacggt
    33301 accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact tataaactgc
    33361 atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga aacggcgttg
    33421 tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa
    33481 caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat atcaaattgg
    33541 accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt aaaggaatcg
    33601 gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac ttattagata
    33661 aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt caatttgtaa
    33721 aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat tatgaatacg
    33781 gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat gctttggatg
    33841 caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc gaccagggaa
    33901 cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc ggaagcggtg
    33961 tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg
    34021 gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc cgcgaagtag
    34081 gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt gattctgtag
    34141 ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac tcgatcgatg
    34201 tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat ggcgaactaa
    34261 tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat
    34321 tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg ccagaaaacg
    34381 cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca acattttctg
    34441 tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat
    34501 aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg
    34561 ataataataa cttgaagagg tagtgaaaga gacgtttagc cttattgccg ataggctgtc
    34621 tcttgaacac tgtttattca gaataaggga ctgtgacaaa aaccgtgtca cagtcccttt
    34681 ttccgaacaa acggtgttca aaagcttgca tctacggcac taagcccaat cagatgaaaa
    34741 atatggagtg ctatttttat ctgattgttc ttactgcttg atgcagagca gctttttcac
    34801 aacctctcca ctaaacggtg ttctcctgcc aactcctcgg tcacaagtca caatattcaa
    34861 caaacatgag aagctgtttt ttgaaattgt tccttgtgac tcggagctaa acggcaggct
    34921 cacaacctct tggtaaacgg tgttcaatca gctgaccctc ggtattagct caaaaaacgg
    34981 caaaatatga ggaactattt tttccgtttt tttctctaat actcaggtca aaacgctgat
    35041 ttcacaacct ctgattcacg gtgttccaga agtagcttct acggaaataa gccgaaattt
    35101 cacaaaaatt tgagaagcaa ttttcgtaaa ttccttctta tttcttgaag ctgaccactt
    35161 ctgtcacaac ctcttatttt gattagaact tgcgaaaata aaccggttaa agtgtgtcta
    35221 gcgcaagaga tttgctataa tagacgataa gggtaaaatg taacgagaag gggttgaaaa
    35281 atgtgtatca aattgtaaca gattcttgtt gtgatctgcc ataccatgta ttagcaaaag
    35341 aaaaagttga ttttttatca atgaatctcc aattgaatga tcaagagtta gtagatgacc
    35401 tcggaaagac attcaattat gaccgctaca tgaatcagct gaaaaatggt gcgatgccta
    35461 cgacatcaca aatcaatgtt ggccgatatt tagaattttt ccgttcttat gcggaaaaac
    35521 agattcctgt tttatatgtg gctttttcca gtggattaag cggctcttac agtagtgcgc
    35581 ttcaagcggt tgaattgctg aaggaagaat atgaagaccc tgagatctat gtttttgata
    35641 caaaagctgc aagtattggg gaagggcttt tagtattgga agctgtaaga ttgaaaaaag
    35701 aaggtaaaag catcgaagaa accctagagt ggctggaaag taattatttg aaagtccatt
    35761 catgggtaac agtagacgat ctgaaatatc tagaacgagg cggtaggatt tccaaagcaa
    35821 gtgctactct tggcagtcta ttgagtgtaa agcctattat tacggtggat aaagaaggaa
    35881 ggctgacgaa tgttgacaaa gttcgaggaa gaaacaagtc gatccagaca atcgtcaatg
    35941 agacggtaaa aacaatcgtt gcaccacttg atcaaatcgt ttatgtggct tacgctgggg
    36001 atctagagag cgcggagaag gcaaaacggc ttttggaaga gcagataaag atgaaagatg
    36061 tgaagcttta tcccttagga ccaacgatcg ccagtcacac aggctatggc tgtatcgcga
    36121 ttttttcgat gggaatcagt cgataaaagc agtaactcat tctgtaaaaa agcccagaaa
    36181 ttgattgtaa caaaaacagc gactcttaaa agatagatga gcatcctatc tcttagaggt
    36241 cgctgttttt ttatttttta agctaaatag gccattatgc ccataactcg ttcaattgtt
    36301 tttgaatatc tgcattttcc aaaaattcat catatgtcgt atcagctcga tcgatcacac
    36361 ctttatccga aacggcaatg atgcggtttg ccaatgtttg gataaattga tggtcatgtg
    36421 acgcaaataa gattgagcca gtaaatgcca tcaagccgtc attcaatgct gtgattgatt
    36481 ctaagtctaa atggttcgtt ggatcatcta atacaagtac gttagctttc gaaagcatca
    36541 tttttgaaag catgcaacgg actttctctc caccagataa aacattcact ggtttcaata
    36601 cttcttcacc agaaaacaac attcttccta agaagcttct taagaaggtg ttatcatctt
    36661 cttctttact tgcatattga cgcaaccagt ctaaaatcgt tagatcagta tcgaattctt
    36721 tcgttgtatc ctttggtaaa taagaattac ttgttgtcac accccaacgt actgttccag
    36781 tatctggcgt aatttcaccc atgataactt tgaatagcgt tgttgttgtt atatcatttt
    36841 ctgcgacgaa agcgaccttg tcttctttgc tcaaattaaa agatatatca tctaaaattt
    36901 tctttccatc aatcgttaca cttacatttt ctacctgcaa taagtcgttt ccaatctcac
    36961 gttcaggttt gaatccaacg aatggataac gtctagaaga aggttggata tcgtctaaag
    37021 taattttttc aagcattttt ttacgtgaag ttgcttgttt tgattttgat gcattcgcac
    37081 taaagcgggc aataaattct tggagttctt tgatttgttc ttcttttttg gcatttgcgt
    37141 ttgcttgaag tttagccgct agttgactag attccaacca aaaatcataa tttcctacat
    37201 aaagtttgat tttaccaaaa tctagatccg ccatatgtgt acatactttg ttcaaaaagt
    37261 gacggtcatg ggatacaacg atcacagtat tttcaaaatt gatcaaaaat tcttccagcc
    37321 aattgatcga ttggatatcc agaccgtttg ttggttcgtc tagcaaaaga acatcaggtt
    37381 taccaaatag tgtttgtgct aataacactt ttaccttttg tccaccagtt aattcactca
    37441 tttgctgatg gtgcaattct tcagggatgt tcagaccttg cagtaaaaca gctgcttctg
    37501 gttcagcttc ccatccatta agctcagcaa attctccttc taattcagct gctcgaatac
    37561 catcttcgtc agtgaaatcc tctttcatgt aaatcgcatc tttttctttc agcacttcat
    37621 acagacgctt gtgtcccatg atgactgttt caagtacagt atagtcttca tagtcgtaat
    37681 ggttctgttt caatacggcc atccgttcat ctggtcccat agtaacggaa cctgtggaag
    37741 gctgcagatc ccctgataac actttaagga acgttgattt acctgcacca tttgcaccaa
    37801 tcaagccata gcagtttccg ggggtaaatt taatattcac atcatcaaat agtttgcggt
    37861 ctgaaaagtg caaactcaca tcatttacag taatcaattt ttttcctcac ttttatcatt
    37921 attagttttg acaagtctta tagaattata taggggaaag gtaaaagatt caaggcaatc
    37981 gaacattctt tgtgtttcac gtgaaacaga atgaaaaaaa taaaaataaa agcggagcga
    38041 aaatagctac tataggattt attagtttgc aacagcataa tttttgacgt tatttataag
    38101 agggagtact ataaaactgt aaaagaaatg aaagaaggat tgaaaatgga tgaaaaaaga
    38161 accatcgact ggggctcatt gattttggga attttatttg tacttgtctc attgttatca
    38221 tttcaagacc ctgtcggaaa tttagttgca attgtagtag tctttgcgat ttttgccttt
    38281 attaaaggga tatttgaact gtttgtacgg aacagattaa aagagctaac aggatataaa
    38341 ggaaagatgc ccttaataat cgggatcatc gatattctag ttggcgtctt tttcttattc
    38401 aatattggtg caggagtcgc agcattgcct tttgtttttg cagtatggtt tattgctgat
    38461 tcagttttag cgttatttac tgctgacttg gcaagaggag taagtgaagg atattattgg
    38521 tttacgatca tcgtcaatat tttaggaatt ttattaggaa ttttcttatt gtttaatcca
    38581 atttcttctg cgttgacatt aagcttttta gttggattct attttatgtt atttggtatc
    38641 acacatatcg tctatgcatt tagataagta aaatcaaaca aataaaaaag ccggcttccg
    38701 attacagttt tactgttgta attggaagcc ggctttttta tcatggacaa atatgcacga
    38761 ggtgattttt aggtgtcgag acaaatttat gcaaaaatgt gagaagctat atacacaaat
    38821 tttatcttaa tactcgaagc atagcgcagt tgtcacaacc tcttttcata taattcattc
    38881 aatactggca aatcatggac ttcatatttt tctttgaatg cttggatttc ttcttcagaa
    38941 tattttcttg gatctaaatg cagatcttct actggcaatg ggcgcttgtt attgatcttc
    39001 acatcgatta ctacaggacg atctgatttt gcagctgcat caaaggcaaa tcgaagttgc
    39061 gagtattctg tgactgtaaa gctatttgcc ccgagcgctt ctccaacttt accgaaatct
    39121 gcatcttcta aaaatacacc aaatttcttt tgttccgtat cttcttgttc tgcttcgatg
    39181 aaaccaaaag aatcattcga aaagacaaca ttaataattg gaagctgata tttgacttgt
    39241 gtaatgatat cttgcatgac catagcaaac ccgccatcac cacttaaggt aaatacttgt
    39301 ttttctgggt agcttagttg tgcggcaatt cctcctggca caccattacc catagttgcg
    39361 aaccatccag atgtcgtatg tttttgttta ccgttcatat tcaataaacg aatagaatgg
    39421 atagtcgtgt taccgacatc tgtgacgaaa atcgcatcgt cttcagcaat acgattgatt
    39481 tctttataga cagcctctgg acgaatcggt tcttcctctc gatcttcaaa agatttcaac
    39541 caatttaccc atttttcttt attttcttga tttgctaggt accagctatc agcagggcga
    39601 gcatccccta actcgacaag tctttgcaaa gctgtgtttg catcaccgag gatcgacaaa
    39661 gatgtttcat ggcgtcgtcc aaattttgac gcatcgatat cgatttggat aaattcagca
    39721 gttggattaa aaaatgcacg tccaaatggg aagtcgctac cgacaaataa gatcagatcg
    39781 gcttctgcca atgcttcatt tccgggttta gtagcgacac gtccagcaaa accaagaaag
    39841 ttttcataat catcaggaat aattcctttt gctagaacgg aagcgacaac aggaactgaa
    39901 aaatgttctg agaattcttt gatttgcgga aatccgtttc gcgttccttg accaacataa
    39961 agaactggtt tttttgcttt ttcaagataa ggcaaagcag ctagcaaatc tttttcttct
    40021 ggtaatatca cacttgtttt gtgagtgtga gcggtggaga aaggttgttc gtcaatttct
    40081 tcaaaaccaa gatcaacagg gatagtcacg atagccacgc ctttatgctc ataggcggct
    40141 ttaatggctt catctactac atgcggaaga ctttgtggtg tcatgactgt acgattgtat
    40201 acgctaacat ctgcgaaaat aggattttca tttagttcct ggaaagagtt atagttcatg
    40261 gatgtagaag caacttgtcc taagaacgct actagaggca catggtccat ctgtgcatca
    40321 tacaaaccat tgatcaaatg tgtcgcacca ggtcctgcag agccgaagac agcgccgact
    40381 ttccccgtta attttgcatc tgcagcggct gcgagtgcgc cgacttcttc atgacgaact
    40441 tgaatgtagt tgatttcggt tttttcttga tatagcgcat ccataattga attgaaagat
    40501 cctccgggaa tgccgtaaat atggtcgata ccccagcttt caagaacttt taccattgcg
    40561 attcctgcat tgatttttga catggtgttt cactccttta gcaatgatga tacataaatt
    40621 ttttgtatct agagtataga actttttatc gaaaatccca actaattcgt cttttcacaa
    40681 aaatgtagtc atgtttttgg gaatatgcta gagtagtaca tagatagatt caagagaggg
    40741 gaatggcatg gttgaatggc tattgagact tgaaccttgg cagcaagcac ttgctgggac
    40801 agcttttact tattttatga cagctcttgg tgccgggttg gtttttttct ttaaagaaat
    40861 caaaaaagaa gtgctaaact tgatgttagg atttgcttct ggtgtcatga ttgctgcaag
    40921 tttttggtct ttgttagatc cggctattac aaaagcagaa gaaaatggag atattgcttg
    40981 gctagttgta agtattgggt ttggtttagg cggtgtgttt ttatatatgg cagataaaac
    41041 actgccccat atgcattttg gaccacaaca tgaagcagaa ggattgccta cccatttaaa
    41101 acggacgatc ttgcttgttt tttctattac attgcataac attcctgaag gtttagccgt
    41161 gggggtagca ttcggtgcag cagcaactgc ggataatccg acagccgcta tattagccgc
    41221 tgtttcggtg gcgctaggta ttggtatcca aaacttccca gaaggagcag ctgtatcaat
    41281 cccgttgcga caagaaggtc tgagtcgtaa aaaggccttt gtttacggac aagcttctgg
    41341 aatcgttgaa ccaattgctg gagtgattgg agcgctacta gtaacgaaag tggagctgct
    41401 gcttccttat gcacttgctt ttgccgcagg agcaatgatt tatgtcgtgg tggaagaatt
    41461 gatccctgaa gcacagcaga cattgtccag caaacgtcat tttgcagttt ttggcgtcat
    41521 gagtggattt atcattatga tgattttgga tgtggcatta ggataaataa aaaaaatata
    41581 tatgatatat caaatagggc cgcgaactta tatcgcgacc ctattttttt agtaagttat
    41641 atcataatga agtgaatggt atcatacatt aataatagtt ttttctatgt gtatttcctt
    41701 tgatccagcg aacaagccag atgataagtg cgattgggat tagaactttg accgcaaacc
    41761 ataaaagccc gcttaggaca ctgacaaaaa gactgatgac gaagctagct gcaattagca
    41821 ctaaaagaac catgaagata tttaagaaca tatttttttg acgcatacaa aaccctcctc
    41881 ttgttcattc tttactatag aaaaagtata acagggattg tttaaaaaca cctccaactg
    41941 aagactgatt tttacttcat ctacttaaat gatcaaacaa ttgctttttc tctggtttat
    42001 catctaaaat agagcggaaa aggaggagaa tatcatgcct aaaaatcgtc ttgaagcatt
    42061 tacagatgct gtaatcgcga ttatcatgac tattttagtt ttagaacttc atgaacctaa
    42121 cggagacacg tgggaggcgc tcgctgcatt aggttaccga ttttttatct atattgtcag
    42181 ttttatcatt ttagcgattt attggaacaa tcatcatcat ttatttcaag ttgtacataa
    42241 gataaatgga cgggtcttat gggcgaacag tttctttgtt tttgcgcttt cgctttttcc
    42301 gtttgcaact tcatggatca gtgagtacat caatagcctg gctccagaaa tgacttatgg
    42361 ttttgttacc ttgaccgcta atattgcgta ttatatcttg gcaaaagaac ttgtcaaagc
    42421 aaataaaggc cacgagaaag cattgaaggc gatgttgaat aattacaaga aatcgtatct
    42481 atcgattggt ttgaatgtgt taggaattgt cttagggtat ttagtagcgc cagtagccgt
    42541 gctgatcgca aacatcgtga ttctagctgc ttggatcatc ccaagtcgta ccatcgaatc
    42601 acaatatcgg taaaatgatc cgtgtttaaa atttaagaga aaaatataga aaaaataaat
    42661 aaacgatcaa atatccgagt gatactagta ttcaaagagg gatgcagatg gatcaaaaaa
    42721 gaattcatgt aggactgatt actagttttg tcacgatgct tatttatttg gatcaaggtc
    42781 atttgacttg gacagtagtt ggtgtcgtag gatttttgac attattcaca gcaataaaaa
    42841 aataaacatc tttatgtaat ctgatgttct atctatttaa gaaaagcatg tttacagcag
    42901 tatgattctg ttgtaaacgt gtttttattt gtggaaaatt taatttttag aaaagtgtat
    42961 cgaaaaatag atagttagtt tttccacagt ggataacttc gtaagagata aaaataaaaa
    43021 attagtcaaa gaagggcgta tagatttctt ttgataggag atatacaaca gaaatgaata
    43081 aatattaacg tgttttcaaa cagtataaaa cggtgtggtt tttggaataa aaaaataaca
    43141 tatctcattt tacacttttg ttattttata cttaaaaaat aacgacacag aaaataccgt
    43201 gtctcagatt tctctactac ttctaaaaac tactgttttt ttcctttgaa atgccttcaa
    43261 aaagtaggat caggcagaat atactggcag aatcaaggag aagggagcct aaataaggag
    43321 aaaaaccata gaaaatataa agtaattact tataaaacaa gctttttata gaaaaataat
    43381 atacctgagt agggtataaa ttaagacgga attcctttct agattgagat attgactagg
    43441 aagaatgtta agattttaag cctttaagct aaaaaaaaac cctttttgtt aaataagggg
    43501 aatatagcct gtcagtttca aactgcaagt cgtcctgctg caataatata gtagattaga
    43561 gatgaaaata gattttttta aaccaatgac tgaataaaaa gaccgtcata gatacacatt
    43621 ggaatgtgtc tattacggtc tttttttacg aacgatttta ttgaagacgg acagttcggg
    43681 tcaaaaataa aaaagctacg atcagaaata agaataaaat gtatcccaaa ataatggcta
    43741 gacggtctaa cttatttgtc gatagatgtt tgtttagaag ttgataggcc cataaaccta
    43801 tcagtcctcc tgttgtatta gttatcaaat cagtgatgtc cgctacgcca ataccgaata
    43861 ggaattgaat caattctgcc aagaagctta caaggaaaat aatccctaat tttttagaaa
    43921 aagaaagtct cttgaaattg acattcaaaa gcaccccaaa aggaaaaaag acaatcaagt
    43981 tataagtggt ctcacgtaat accgtcgtat tttcaaaaga aaaagtagaa aaagggacga
    44041 gattgacatt gctgtaagaa taagtgagaa attgtggaag attaaatgat agcttgaata
    44101 aaatcaacca taatagaaag aataaataaa tccctaataa aaattttgat acataccgca
    44161 tagacatctc ctccatttaa aaagtatacc atcgaaaacc gctgaaaaat cataaaaaag
    44221 tatgaagttt aaggaccaac gtagttatcg tacgaaaatt agaagctttt ttttgttggg
    44281 aatccggttt ctttaacaaa gctaagatag cgagttgctt ggaaaagttg ctatcttttt
    44341 tctgtcaaaa cgactcggaa tgttcgtttt ttgttctaat ttctccaagt tgtttacttt
    44401 ttgatactaa gtttatgaaa aatcaagaaa tagttgttat actaggtaat gctgacaaac
    44461 gggattggca tttcatcttg gttcagcttt ttttccaatt actaggaagg agggatagat
    44521 tgttcaagac ttattttcgt ttcaataaaa tacaagataa tccttttcgt atcatgaatc
    44581 tgatgctact tatctttatt gtattatggt taggttacat aaatcatgat tttttgattt
    44641 caagtttttc atcattaggg atctttactt ttttctatta tcaaaacata ccgatgaaat
    44701 atttgctgaa acgtatgttt ctgatcggtt gcggactgtt ggttgctttc gttttgggga
    44761 tcttatcgac ttatgtttta tggcttgaac catttgccgt agctttggtt gctttttcca
    44821 gcagattcgt gttgcgatta ttccatattt ctaaaccagg tggcttgttt tttgcgatgc
    44881 tttctgccat gggaacgagc atgcagctgc caatcgctca attaccgatc gtctcacttt
    44941 atttttttat gggtgtcgtt tttgccctga ttgcggcagt gattacaaaa ttactggatt
    45001 cacggcctga gcagacaatt gaaaaagcaa ctttaaaaga aagatttcac gaagagcctt
    45061 tagtgatcat tgattctgtt ttctacagcg ctgccttatt tctttccgtt tatgtcagtc
    45121 acggcttgaa tctgcataat ccttattggt tgaccttgtc atgtgcatcg attttgctag
    45181 cagaaaactt ggatgcgatg aagcatcgac aagtccaata tctgattgga tcgatgggtg
    45241 ggttatgtgt gtcggcattc ttgtcatttg tgccgtttac gcagttgcag accatttttc
    45301 tgatcacttt tctatatgga atcgcacaat ttctagttgc tagaaattat gcagttgcta
    45361 atatttttct taatccgatg gcgttgatgc tatctacact tattcgtgga gcatacttga
    45421 tttctttgat cgaatatcgt tttttaggga tcgtcatagg aagtttcatc ggtttaggtg
    45481 ttgcgtgggt aatgacggtt ggtttgcaac attatttaac ggttgttcgt caaaaattag
    45541 aatagaagga caaatatatg tgccaaaaga ggttatgaca agatttttgt cacaacctct
    45601 aattcaaggt gttcaaaagc cagattctca gcattaagtc aaattcacac aaaaatgtgg
    45661 aaaggaactt tcaaaaaaat tttatattta ttctatcaaa aaattttgtt tatgatggta
    45721 actgaataaa ctttcctgag atggtcaatg ctttccttga aagtcttaca gatattcttt
    45781 cttcatagaa agtttaagtt ttatagttag tgtacaataa aagtatgttt ataccatatg
    45841 gtgtccggat aatcagtcat ttaaacaatg aggttcaaag atatgaaaag gggaaatttg
    45901 atgaagaaaa taagcaatga aatccaaatt ctggcagaaa ctttaactct tcccaaagtc
    45961 gttatagagc aaatgattgt acaagcaaaa aacatccttg atgaggtgtt tgagcaatat
    46021 agtcatgaac taatggatcc taataaagca aaggcgactg caaagaaaat agacaaagtc
    46081 tatgaagagg aaccttttgc agcattagct atttatctat atgcagcttc tcaaagttgg
    46141 aaaaaaattt atcaaccatt gaagattcca agaactatct atttggcaac aatgaatgct
    46201 tttactagat ttattcatga acatttccaa gtaacaggaa gctatcgctt tgaccgtggt
    46261 ttttggattt ggcattacac tagtggcttg atctttcgga ttggtgaatt ggagtacgaa
    46321 atgacatatt ttccgaaagg gtacacggat ctaagattag aaggaaagca gtgcctttct
    46381 cttcatattc cttccgatgc agatttatct cagaataaga taagagatag ttacgaaagt
    46441 gccattcgtt tttttgaact ttattttcct gattaccatt accaagtgat gtacacagat
    46501 acttggttac tatcacccaa tcttactaaa tggttgaaaa aagagtcaaa aatctgtctt
    46561 tttgcagccg attatcgatt attatcagtt gatgaacagg acgatagtgg agtcccttgg
    46621 atctttggac gagtagatgc ccaaatacat gattatccag aaagtacgag tttacagcga
    46681 caagcaaaag agcaattgct tgcaggcgag cacattggct ctggcttagg tattcttact
    46741 tcataagttt agtacttcga aatagtttaa aagagcgtga gacaaaagta aagaacactt
    46801 ttgtctcacg ctctaaacac gtataaacgg cggaagcaga agcaactcct tcggaaataa
    46861 gctgaaattc acaaaaattt gaaaagcaat tttcgtgaat ttcttcttat ttcttggagt
    46921 taaacgcttc tgtcccagcc tcttttttag tgttctatcg aacacacagt acgttcaata
    46981 ggtaaactag ctataacaat agatattaaa gttaacggac aagagggcct tttttaggta
    47041 gctaaaacgt aataatgaaa atttatttcc agagatagga gtcaatcagc tagtctgatc
    47101 gactgtttta acaagttgac tttcatttta acttttgata ccatttaaaa cacaaatcgt
    47161 aattgtttcg atttgtgttt tttagcatga ctacaatcaa agaagaaagt gggtcgtcca
    47221 tatggaaaaa acagatatct caagtgcata ccgccgcttg aaaagcccaa atatcaaaac
    47281 acgaaaacgt gcgctgaaga tcatcaaaga tgtgaaacgg aatagtggga aaaggtaaga
    47341 agaaggaaac ttaattaaaa aaatagccga aaaacgtttt tcggctattt tcaataatcg
    47401 gtttttgtgt aaggtttccg aaagaactgc tgttgatttt ccatttttaa catatgataa
    47461 attggtagaa tctttctttt caaattgata ccattcccta attccagtta tatagttaac
    47521 tacagaacca caaagcacgt acgtttataa aagtattatc tgtagttatt tttcaattcc
    47581 ctttcaacct cttattttga ccagtagtat tgatcacttt accttttaac acctttgttc
    47641 gtttttgaaa catttttagt caagaatgga aacgtaacta cataatcaaa ggttttgcga
    47701 aatatttcat gaaatatttc atgtagaagc gagcaatacg gttgcaaata accattatcg
    47761 tttaataagc agttacgaaa gctcccaata tctctaacgt tcagctcact ccatttcttt
    47821 tcttcaaaaa aatcagtgat tcgtatctct ttctatttgt cgaaaaagtt aactttaaaa
    47881 agtaacgccg aaaaaatatg caaaaaaata aaaaagcagt tactattgct agaataacat
    47941 caaggaataa taagttttgg ttttcagata atagtcgata agttgtgtat tggaatttta
    48001 gatacagtaa taaagtattt ttcatgaaat tttgtgagaa tcactattac aagtatcttt
    48061 aataaaatta atggttaagg aggaacttaa agtagaatgt aaatgaatag ttgtagtcag
    48121 tcgattgtga tactagactc aaagttgcag gaataagttt ttcaatttgt gattggaaga
    48181 taaaaaatga aaaagaaggg ctttttttta tactttgttg attgtagtgt ttattttccc
    48241 tctgtttagc caagtaacat tttctatgac tcaaccagtt tcaagtgagg aacatgataa
    48301 cctttttcgt gctacagaag agtttaagaa atatcaggaa aatattgttc cggagactcc
    48361 attagctagt gagccaatta ctgatattga tggaaaccaa ataggatggt atatacagta
    48421 cgaagttatg gtggtaaata atgactcaat agtaaaaaat acagtaggct tagagctaac
    48481 accgattgaa aaggtgagtt cattgttaac gtatatttat actgatgaaa gtgacgagac
    48541 tgagatcatt ttaacggatt actcaaaaat tgctactgat aaaaagtata tattgtggat
    48601 gtagacacta gtgaaatact ttatgatata gaaattgatg aaaatttact agatgtagca
    48661 caagaaatcg atcatcaaaa gcaggaagct attcgatatg ctggtagaaa agggaaaaga
    48721 aaatatttat tacgtgaatg tagcaaaagt tagagaagat gaaaatgagt ggaaagaatt
    48781 caagagtcgc tactcaatta actcgactcc gacatttaca gtttacagag aaggatcgat
    48841 agaaaaaact gttttttgga ctaaagaaag tggaatgtct ttagctgaag tagaagagtt
    48901 tttggattat gttagtatgc aacagtaagg gagttcttat
//
LOCUS       DACDUU010000014        44635 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.014, whole genome shotgun sequence.
ACCESSION   DACDUU010000014 DACDUU010000000
VERSION     DACDUU010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.014"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..362)
                     /locus_tag="IWL30_000819"
     CDS             complement(<1..362)
                     /locus_tag="IWL30_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705607.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEA"
     gene            complement(472..2331)
                     /locus_tag="IWL30_000820"
     CDS             complement(472..2331)
                     /locus_tag="IWL30_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP9705608.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(2449..3609)
                     /locus_tag="IWL30_000821"
     CDS             complement(2449..3609)
                     /locus_tag="IWL30_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAP9705609.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(3916..5340)
                     /gene="araA"
                     /locus_tag="IWL30_000822"
     CDS             complement(3916..5340)
                     /gene="araA"
                     /locus_tag="IWL30_000822"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAP9705610.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(5356..6054)
                     /locus_tag="IWL30_000823"
     CDS             complement(5356..6054)
                     /locus_tag="IWL30_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP9705611.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(6011..7672)
                     /locus_tag="IWL30_000824"
     CDS             complement(6011..7672)
                     /locus_tag="IWL30_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAP9705612.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            7890..9290
                     /locus_tag="IWL30_000825"
     CDS             7890..9290
                     /locus_tag="IWL30_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAP9705613.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(9542..10621)
                     /locus_tag="IWL30_000826"
     CDS             complement(9542..10621)
                     /locus_tag="IWL30_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9705614.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(10707..11666)
                     /locus_tag="IWL30_000827"
     CDS             complement(10707..11666)
                     /locus_tag="IWL30_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAP9705615.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(11678..13201)
                     /locus_tag="IWL30_000828"
                     /pseudo
     CDS             complement(11678..13201)
                     /locus_tag="IWL30_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
     gene            complement(13211..13774)
                     /locus_tag="IWL30_000829"
     CDS             complement(13211..13774)
                     /locus_tag="IWL30_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP9705616.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(13780..14643)
                     /locus_tag="IWL30_000830"
     CDS             complement(13780..14643)
                     /locus_tag="IWL30_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9705617.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(14657..15553)
                     /locus_tag="IWL30_000831"
     CDS             complement(14657..15553)
                     /locus_tag="IWL30_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9705618.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(15728..16999)
                     /locus_tag="IWL30_000832"
     CDS             complement(15728..16999)
                     /locus_tag="IWL30_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9705619.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(16989..18506)
                     /locus_tag="IWL30_000833"
     CDS             complement(16989..18506)
                     /locus_tag="IWL30_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP9705620.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            18704..19594
                     /locus_tag="IWL30_000834"
     CDS             18704..19594
                     /locus_tag="IWL30_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAP9705621.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(19667..20491)
                     /gene="nadE"
                     /locus_tag="IWL30_000835"
     CDS             complement(19667..20491)
                     /gene="nadE"
                     /locus_tag="IWL30_000835"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAP9705622.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(20503..21975)
                     /locus_tag="IWL30_000836"
     CDS             complement(20503..21975)
                     /locus_tag="IWL30_000836"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAP9705623.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(22612..22974)
                     /locus_tag="IWL30_000837"
     CDS             complement(22612..22974)
                     /locus_tag="IWL30_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP9705624.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            23197..23949
                     /locus_tag="IWL30_000838"
     CDS             23197..23949
                     /locus_tag="IWL30_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9705625.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(24089..25012)
                     /locus_tag="IWL30_000839"
     CDS             complement(24089..25012)
                     /locus_tag="IWL30_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9705626.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(25027..25299)
                     /locus_tag="IWL30_000840"
     CDS             complement(25027..25299)
                     /locus_tag="IWL30_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705627.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
     gene            complement(25769..27394)
                     /gene="groL"
                     /locus_tag="IWL30_000841"
     CDS             complement(25769..27394)
                     /gene="groL"
                     /locus_tag="IWL30_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAP9705628.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(27445..27729)
                     /locus_tag="IWL30_000842"
     CDS             complement(27445..27729)
                     /locus_tag="IWL30_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAP9705629.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            27954..28616
                     /locus_tag="IWL30_000843"
     CDS             27954..28616
                     /locus_tag="IWL30_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP9705630.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            complement(28692..29984)
                     /locus_tag="IWL30_000844"
     CDS             complement(28692..29984)
                     /locus_tag="IWL30_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9705631.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            30154..30783
                     /locus_tag="IWL30_000845"
     CDS             30154..30783
                     /locus_tag="IWL30_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAP9705632.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            complement(30886..31695)
                     /locus_tag="IWL30_000846"
     CDS             complement(30886..31695)
                     /locus_tag="IWL30_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9705633.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(31750..32619)
                     /locus_tag="IWL30_000847"
     CDS             complement(31750..32619)
                     /locus_tag="IWL30_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705634.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            complement(32620..33936)
                     /locus_tag="IWL30_000848"
     CDS             complement(32620..33936)
                     /locus_tag="IWL30_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705635.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            complement(33933..35768)
                     /gene="walK"
                     /locus_tag="IWL30_000849"
     CDS             complement(33933..35768)
                     /gene="walK"
                     /locus_tag="IWL30_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAP9705636.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            complement(35773..36477)
                     /locus_tag="IWL30_000850"
     CDS             complement(35773..36477)
                     /locus_tag="IWL30_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9705637.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            complement(36667..37527)
                     /locus_tag="IWL30_000851"
     CDS             complement(36667..37527)
                     /locus_tag="IWL30_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9705638.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            complement(37514..38083)
                     /locus_tag="IWL30_000852"
     CDS             complement(37514..38083)
                     /locus_tag="IWL30_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAP9705639.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            38464..38536
                     /locus_tag="IWL30_000853"
     tRNA            38464..38536
                     /locus_tag="IWL30_000853"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38497..38499,aa:Lys,seq:ctt)
     gene            complement(39032..39418)
                     /locus_tag="IWL30_000854"
     CDS             complement(39032..39418)
                     /locus_tag="IWL30_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP9705640.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(39415..39936)
                     /locus_tag="IWL30_000855"
     CDS             complement(39415..39936)
                     /locus_tag="IWL30_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP9705641.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            complement(39938..40972)
                     /gene="sppA"
                     /locus_tag="IWL30_000856"
     CDS             complement(39938..40972)
                     /gene="sppA"
                     /locus_tag="IWL30_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP9705642.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            complement(41005..42420)
                     /locus_tag="IWL30_000857"
     CDS             complement(41005..42420)
                     /locus_tag="IWL30_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAP9705643.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            complement(42389..44542)
                     /locus_tag="IWL30_000858"
     CDS             complement(42389..44542)
                     /locus_tag="IWL30_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HAP9705644.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
BASE COUNT        13057 a         9309 c         7371 g        14898 t
ORIGIN      
        1 gcttcagatg tattgaatag atcttcatct acccctgatt caagatcgat actttggatc
       61 atttcttcac agcctgctac atatgaaaga taagcttttt caaaattttt atgaatgccc
      121 attgcttttg ctggtggacg caaagtactg actttcttaa gcattgcccg atattttttt
      181 gttccttctt caaataacag gatgatctct tcaagagtcg atttgtccag ttcacttgtt
      241 tttccttcat caattgcgtt tctgatcact tcatatttcg gatgcattcc ttctccgatt
      301 gcttctgttt ccttgacgat ttgatcgatg gactgcacat aatagccaat attcggtaac
      361 atgttcgttt tcctactttc tccattgttg ttattattta ttctacttgt ttttatcctc
      421 aaatgacaag tacttgaaaa aatttaatgg tttctataca gctcgccatt cttattcgtg
      481 ctcaaagaaa ccatctgcta aactatattg tttccctgcg acaatgtcat attggatcaa
      541 ttgatattcg tcgtaaatct cttgttgcga gcgattgaat tgcgcttctt tataccactg
      601 tccgttttga tagtacagtt ctcgttcaaa cgccggaagt tcctgttcta atgataaaag
      661 cagctgataa aagccatttg ttgtctgatt cgtctgttcc attagattcg ctgcaaaata
      721 aaacggactg gtgattgcat cgtgagtcgt ttgcttttct aattctcctt tactgtcgaa
      781 cataagaaat tgtgtttcat gcaatgtttg tttgtcattt tttgcttgga tcgtatctga
      841 atagatccct ggaagatgat ccccccaaaa aacaactaaa gtccttctag aaagattctt
      901 caattcttcc gtgaactgct tcagtgccgt gctgctatag gaaatatctt gcagataatt
      961 ctctaagtca agtgtcccac ttccatctga agtttttgca ggatagtcta gattatcata
     1021 ctttccatcg tagggcatat gtgtctgcat cgtgactaaa tgaatgaact gaggtgtttt
     1081 ttcttccttg agaagtgtca ttacttcttt gtaagctgac tcatcagaaa tatatggatt
     1141 attctctatc gtatccgtat aggtcatcgt acgctcgctg atgaactggt caaatcctaa
     1201 tgtctgataa acatcctctc ttttatacat cgaagtattg tatggatgga tcgctgtcgt
     1261 ctggtatgat tgtgcgttta acgctgaaac gatagaagga agctgatcca tttttggtac
     1321 aagcatcgtg taaggggtgg tgagttgcgc attaaataac gccatagaaa aaccagtcaa
     1381 tgcttcaaat tcgatatttg ctgttccacc gccatagttt tgagaaagca tattgccact
     1441 atatgtttga tcagctactt cataataatc tgccaatggc tcaccagata cttctacgcc
     1501 gtttaatcga ctaggatctg agaaactttc actcatcacg aacacgatgt tcggcgactc
     1561 ttcctctaca gttttatttt tttcatcggc tagcttctgg tatttttcag tgatttcttt
     1621 gatcttttct ttcgaatatc cttcaggttc atccattggt tctaccttca gattgtacaa
     1681 aaacccaccg ataaaaccag tgttatagta gttcattttc tggctgtaag gtatccatag
     1741 tgccgtttta ttatatgctt ttcgcaacaa attatcggga ttgttgaaat tactgatata
     1801 ccctagcagt ccaattgcca caagcagtgt cgttaatcgg atcaattgga atcgccttcc
     1861 ttttttcagg cttcgaaaca tgtaccagca aaacaatcct atcacggttc ctgcggcagc
     1921 taagatcact gtaaacagca tagtgccggt catatctttc agcaatccga tttcagtgat
     1981 catcttcaaa tcgtctggat aaatcggttc ttgccgataa ctcattttca tataatttgc
     2041 aaaacctaat acaccgatgg ctacgacata cgtaagggaa cctaaaggga cagatcctgc
     2101 tagtgcaatc agaaaaataa gaataatcag caagacgaga caggctaaga agaatttttc
     2161 cgtatgccaa gaaaaagcaa atttcaacgc aaggtctacg gatagatcat tttgacacca
     2221 ctgcaaaaat aaattactaa aaacaacaag aaaaaagata ctgatgattg ctgctgttcc
     2281 ttttaaaatc tttttctgtt tggtcgaaag tctgcttata tatcttttca aatgcttgct
     2341 ccttcatttc tatcaataaa agaatgttgc ttctcttatt ttagttgtac gaaattcgaa
     2401 agtaaagaac ggaaagaaga attaatgaaa tccaaatatt ctctttcttt atggcctttc
     2461 tggtttcttt aataagcgga cctttcgtac tccagaaatt ttttctagct gttgtttcac
     2521 ttgattgagc tgttcaaaat cagtactgtc tatgtcaatc aatatgtaga catgctcatt
     2581 cttatggtta cgcgatatat ctgcgatcgc tagctcgttc tcattcagca gagaaaatat
     2641 ctctgctaat atattggttt tcttttgata aaatatagtg aatcgaaacg gcgcttggaa
     2701 aaacattcgt gcggatggat aattgaccgc ttctctaacc gtaccgaaca aaagataatc
     2761 ttttaaggct tcaactgcca aacgtccgct gtctgccaaa gcttcctgag tcgatccccc
     2821 tagatgaggc aacataagta tacggtcatt ttctaagaac tcttcttctg gaaaatcagt
     2881 caagtattta gccagatgtt gtttttccaa tgcttgcata acagcatatt tatcaacaat
     2941 ttcacttctt cctacattga taagtacagc atcttttttc attttttcta tattcgcctg
     3001 atcgatcaat ccttttgtat cttctgtcaa cggcagcatg actacgatgt aatccgaggt
     3061 agaaagaact gtttctaaat ctgcttgccg aacataatcc aactgggcat cgcggataga
     3121 ataacccaag acatccatcc ctaagctata gcaacttagc gctacttttg tgcctatcgc
     3181 tccaagacca agaagaccga tggttttgcc ttgcaattct cgtcccacat aagcacttct
     3241 tttgttttct gcctgttcta aaatattagg acctgttagt gtttgtacca tacgacttgc
     3301 ttcgattatc ggacgggaag ataagagcaa acagcacaaa acaagctctt ttactgcatt
     3361 agcatttaca cctggtgtat tcatgacgat cgtcccgttt tcagaagctt tttcaacgtt
     3421 gatcgtgttc accccaacac cagctcttga aattgctagc aaatctggtg taagccattc
     3481 atctttcact ttcgtactac gaataactat tccttcggct ggcaaatcat ctattatttt
     3541 gaaatattcc ccttgtaact tttctatctc ttgttctgga atagcatcaa tcaaatgaac
     3601 gtgatacata aaaccccctc cttactaaca agtatctcga aatttctgca attagacaac
     3661 tgtttaccat ttgacttcct agaagcaaaa agagcagtta gagcagagta ggctgagtga
     3721 taaaaataag gctatgacaa gttactgcca taagccttat tttcgagagc aaaatattga
     3781 gggtgagata tcttgataaa tggtgttccg ttagctgaca ttgataataa gactaaattt
     3841 caaaaaagtt gaaaagcaaa tttcgtcaat ctcttcttat ttcttaaaac taagtggctt
     3901 tttcacaacc tctttttatc taataaccac tgtttccaac tcaactaatt ttgcccacgt
     3961 ttcaatttga tctgttgtta gatttagcga gacaacggta tgatgaccgc cacctgcctg
     4021 aatccatgaa tggacacctt catggaaatt cggtttagtt ttccataaca cacgtgcaac
     4081 aggtaaatta ggtgcttcag tcgttggttc aaaggcttct acttcattga tcagcagttt
     4141 ataatgcgta ccgaaatctg ccatcgatac gactacccct tctcctgctt tcccatcaaa
     4201 tacaagacgc gccggatctt ctctatctcc cattgataaa ggagaaacaa cgattcttgg
     4261 tttcgtcgca gcaagcgtcg gatctacttc catcatatgg gattccaaga ttgcttcttg
     4321 gccactagct agctcgtaag tataatcttc catgaatcct gtatctttcc ctcgagccat
     4381 gattttcagc aaccgatcaa tcgctgcagt tttccagtcg ccttccccag caaaaccgta
     4441 tccttctgcc atcaaacgtt gtacagctag tccaggtagt tgtttcattc catacaggtc
     4501 ttcaaaatta gtcgtaaaag cgttatagcc tccttcttct aagaaacggc gaataccgat
     4561 ttcctgctga gcttgtactt ttacatgttc ctcccatgtc ttttcttcat aatcaccata
     4621 gtcgaaggta tacaattcct tgtactcttc aaataattga tcgacttctt cgtctgatac
     4681 gcgatcgatt acttgtacaa gatctccgat accaaaataa tcaacagtcc aaccaaattg
     4741 aatctgtgct tccactttgt ctccttctgt gacacccacg tttctcatgt tgtcaccaaa
     4801 acgagccact tttattccaa aactttcatt ataggctact gcaacatcca tccaatcagc
     4861 aatctctttt tggatctcag gtcgtttcca gtagccaacg acgattttat tttgtttatt
     4921 taatcgagca ttgatgaacc catattcgcg gtcaccatga gcggattgat ttaaattcat
     4981 gaaatccata tcgatcgttt tccaaggaat gctttcgtta tattgcgtag ctaaatgaag
     5041 caatggtttt tgtagaagct ttgtcccgcg gatccacatt tttgctggtg agaaagtatg
     5101 catccaagtg atcacacctg ctacttcttc tcgataattg acctctttca tgatttttgt
     5161 gatcgtatca gcagtaactg ctaattcttg aaggcggatt ggataaggca attggccatt
     5221 ttcattcagt tcatcgacca tttcttgtgc atgtttcttt acttctcgca gtgcttcttc
     5281 cccatataaa tgctgtgatc ctacaacaaa ccaaaattct ttttctccaa tattcaacat
     5341 aattagccac tccttttatt tgttttgtcc gtaatacgca ttttttccgt gttttcgtaa
     5401 ataatgttta tctaaaatcc gttgaggcag ctcttctgaa aatgaattga gctgtctggt
     5461 aaataaattc atcttgcata cttcatctag tacgactgcg ttcattaccg cactttgcgc
     5521 atctttcccc caagtaaatg gaccgtgtcc gtgtaaaagt acccctggaa ttgctaaggg
     5581 atcaattttg cgttctttga atgtttctat gataactttt cctgtttcat attcgtaccc
     5641 gctgtcgatt tcctgttgtg tcaaaaatcg agcacaaggt accgatccat aaaaagtatc
     5701 tgcatgcgtc gtccccatcg caggaacatc caatcctgct tgtgcccaga tcgtcgccca
     5761 tgtcgagtga gtatggacga ttcctccgat ttgaggaaat gaacgatata aaacagcatg
     5821 agtaggcgta tccgaagagg gattgagttt accttcaatg atatgattgt ccaagtccac
     5881 aacgaccatg tcgtctgctg tcatctcttc ataactgact ccgctaggtt tgataacgaa
     5941 atatcgtgtt tcaggatcaa aagcgctcac atttccccat gtatatttga cgagtccgtg
     6001 tttaggcaag tcaagatttg cttggtatac ttcttctttc agttgttcca gcattttcta
     6061 tcactccttt ttcttgcaat tgatgaatcg ctgttttttc aatgtcgatc ccctcacggt
     6121 aacgtctcat aaatatctga tagcctgcta tttcttctgc tgaaggtgtg atggtgattc
     6181 cttggtcttg tccaaacacg tcttctgcca agaagttttc taaagacttc ccatgtcgat
     6241 ccatcatata ggctgctaat aacgcgattc cccacgctcc accttctccg gctgtgtcca
     6301 tcactgtaac tggtgcctcc atagcagatg ctagaatccg ctgtccaact tctggtgttt
     6361 taaaaatccc cccatgcccc actaaacgat caatttggac attctcagat ttcaatatat
     6421 ccattccgat tctcattgca ccaaaagcac tggctaaatg gatccgcatg aaattcgcta
     6481 aatcaaaacg gctacctggt gtacgtacaa ataatggcct tccttcattt actcctgtaa
     6541 tgttttcacc ggaaaaataa ccataagaaa gtaatccccc gcaatccgta tcgccttcta
     6601 gcgctttgtt gaataatacc gtgaataatt tatctctgct tatctccata cccaaagaat
     6661 tggtaaattc ttcaaaaata ctgacccaag cattgatatc cgaagagcag ttattcgtat
     6721 gaaccatgcc tactagttct cctgaaggtg tagtgaccat gtcgatttcc ggatggacat
     6781 tttctagttc tttttctaat acgatcatag cgaaggcgga agtaccagcc gaaatgttcc
     6841 ctgtccgttt ggctacactg ttcgtagcga ccataccggt tcctgcatcc ccttctggcg
     6901 gacaaaccgg gattcctggt ctcaaatttc ccgtagggtc gatcagataa gcccctgatt
     6961 cgcttaactt gccagcttct tctcctgcta ctagaacttt aggcaaaatg tgatccaagt
     7021 caatcgctac gtgcgctttt tcgattaact gaccaaaaat atttttcatt tcttgatggt
     7081 aggttttcgt tgaaggatcg ataggaaaca tgcctgatgc atctcctacg cctagtactt
     7141 tctcaccagt aagctgccaa tggacataac ctgcaagcgt tgtcaaaaaa ttaatttctt
     7201 tcacatgttc ttctttattc aaaatagatt gatacaaatg agcgatactc catctctgcg
     7261 gaatattgta ttgaaatttt tccgttaata ttcgttctgc ttcttcagtg attgtatttc
     7321 gccaagtacg aaatggtact agtaactgtt ccttgctatc aaatgctaga taaccgtgca
     7381 tcattgcact gaacccaatc gacccgattg tcgtaagatt ctcgccatag atttcgaata
     7441 cttctgctgc catttttcgg taactgacgc gcagtccttt ccaaatatct tctaatgaat
     7501 aggtccagat accattttct agtttgtttt cccactcata actaccagcg gcaataggag
     7561 taaaagatga atcaatcaat acagctttga tcctagttga tccaaactca atccctaaag
     7621 atgtacgacc atctatgaca tctaacatac tttgcgcatt tacttccttc ataatgattt
     7681 aactccctcc ttctcgtcaa acctgatcaa accgtctttt tagtagcgct tacaatgatt
     7741 attgtatact tttgtacgta catgtcaagc ggttatttac aagaatacaa taaaaaacac
     7801 ttgttttgta cgtacataag ttataaaatg aaattgctta caaaatctga tcaaccgcct
     7861 tctcgaaatt ctagaaggag agaatactta tgaaaaaaga aaaaaaagtt tcctcatcgt
     7921 ttatttattt ttttggcgca tttggcggca ttctatttgg atatgatatt ggtgttatga
     7981 ccggcgcctt acctttttta cagcatgatt ggggtttggc aggtaaagct tctttgatag
     8041 gatggatcac gtcttcggtc atgttaggtg cgatccttgg cggctcttta tctggtcttc
     8101 tttctgataa gcttggacga cgaaaaatga ttcttctttc tgcacttatt tttatggctg
     8161 gttctgtcct ttctgcatct gcccctcaca atggttctta ttttctgatt gcagcaagga
     8221 ttcttctagg tttagctgtt ggcgctgcat ctgccctagt tcctgcttat atgtctgaaa
     8281 tggctcctgc tagactaagg ggaagattgt cagggatcaa tcaagtgatg attgcttctg
     8341 gcatgctcct ttcctatgtt gcagattatt tgctaaaagt gttgcctgaa acgatggctt
     8401 ggcgcgtgat gttagggctc gctgctgtcc ccgcgctgat tttatttttc ggtgttttag
     8461 ctctaccaga gtcaccgcgt tttcttatgc aatctggccg cttggaagag gcaaaaagag
     8521 tattgaacta tattcggaca ccaaaagaag ctgaacagga attcgaacaa atccagctga
     8581 atgtcaaaca agaaaagaca accggaactt cttggcatac tttgttttta gaaaaatatc
     8641 gttccttagt cattgccggt attggtgtag cagtattcca gcaatttcaa ggagctaatg
     8701 ctatttttta ctatattccg ctgatcgtag aaaaagcgac tgggcatgca gcgagcgatg
     8761 cattgatgtg gccgatcatc caaggaatca ttttagttgc tggttctttg ctctttttgg
     8821 ttattgcaga caagttcaat cggcgaactt tattgaaaat aggcggtagc gtgatgggac
     8881 tttcattcat cctaccagct gttttaggaa ctgtactgga tgctcataca aattcgttgt
     8941 tgattctctt gtttttatgt atttatgtcg cattttattc ttgcacatgg gctccactga
     9001 catgggtaat cataggcgaa atattcccac tagctgtccg cgggcgagca tctggcctag
     9061 cctcttcttt taattggatt ggttctttcc tagttggctt gctttttcct gtgatgactg
     9121 cttctatgtc tcaagaaatc gttttcggca tttttgggat catctgcttt ttaggggttt
     9181 tatttattca agagatcgta ccagaaactc gtgggaaatc tttagaggaa atcgaacaaa
     9241 gtgcatctaa aaaaacttat cctaaacgta tttctatcca tcattcttaa agaggttgtg
     9301 acagaagtgg tcagcttcaa gaaataagaa ggaaccgctt agtttccagt cacaaggaac
     9361 aatttcaaaa aacagcttct catgtttgat gaatattgtg acttgtgacc agttggtagg
     9421 agaacaccgt ttagtggaga ggtggtgaaa gaaacgttca gccttgagcg atgacaaaag
     9481 ctctcacaaa cattgcgacg agtaaccgta ggagcaataa gaggcggtaa aaacccgtct
     9541 cttatttttc gttttctttc actgtttccc gttcaactag tacaggttca tagagaatat
     9601 ttggctgtgc ctgtcctttt tcaatcgttg tgacgatcca ccgtgcggca tcttgtccca
     9661 acttttcttt tggatgcgtc aatgttgtca attttttatt ccctaaatgg cttaaagaag
     9721 aatcgtctgt tcctacgatc gataggtcat ctggaatatg atagccattt tctgtcaact
     9781 tatctagcat taaactagct atttgatcgt tatagcaaat gatgccatct aaagcttgtt
     9841 cttctttcaa ccgttgtact gttttttcag cgattgtttc tcgtgtttcc gtggtgaaag
     9901 taatgatatc ttccgtcaaa aaacgcacat tatactgttc acacgcgctg ataaaacctt
     9961 tcatcctgta ttttccttga agatcgtcga tttttgtgat gaacaacaga cgctgatgtc
    10021 ctttttttat caaatattct gctgccataa aaccgacttt cacatcatct aaacaaatat
    10081 aagaggcatt taactcttca taagcagcat tgatcatcac catcggaatg ccttgttctc
    10141 tcaaagcaac ataaagtgct aagtttgggt tatactgatt acttttcgta ggctcaacga
    10201 tcaaaccatc taccccataa gcaatcattt tcttcaaaca ctctttctcc aattcgtgat
    10261 cgttattcgt actggctaaa agcaatgaat acccagattc tcgtaattca ttttcgattc
    10321 ctcggataat cgacggaaaa atataatcag agagatatgt ggtgatgaca ccgatcgttt
    10381 ttgttctgtt caatggtgtt ttttctttct tatagctgtc atctacatat gtgcccgacc
    10441 ctttttcttt acgcagatag ccctcattta ccaacacagc gattgcttgc cgaatcgtat
    10501 gtctgctgac gtcatatatt tcttgcagct tgaattctga gggaatggtt gtatttactt
    10561 cgtattcctc acgaaggatc ttttgtttta tctcttctgc aatatcttga tattttgcca
    10621 tttgccttgc ccctttttat ttgtacgtac atatatcata acgagtctgc aaggtttatt
    10681 caagcaaaaa tcaaggtttc ttgtgctcat aaggcatacg gaacaggttc accaaattct
    10741 ggaaatcctt gctcattcca cgtaaagcat tgcgcgttcg cgtgccgatt aggattgtct
    10801 aacggatccc ctttttggtt cttctctggt cgagcatgat agatcaaaat atcaaagcac
    10861 ccgtcttctg aaaccgtaaa cgagttgtga ccaggaccaa attgcttatt tttttcagaa
    10921 gaaacgaata ctggctccga taatttatgc catgattctt ttaataaaag attcttgtgc
    10981 tcatcagccc acagcaggcc catggcatag ttttcatctg ttgcactacc agaatacgta
    11041 atgaatattt tcccattgcg agtaaggact gctggacctt catttactga aaaaccccgt
    11101 ttttcccatt catactcagg tatagaaagg aggatctgct tcccctttaa cgtccaagga
    11161 ttatccattt cggatagata aagattagaa ttcccagtaa tttcaggatc ttgttgggcc
    11221 cacacataat atagctttcc ttgagaagag aaagttgttc cgtctaaact gaaagtttcc
    11281 cttttcgttt ttatttgtcc cttttctatc caatctgtat tcattggatc tatatgctga
    11341 ttttcaagta cgaacatgcg gtgatgatga gattcatcac gtatagtttt atgattgctt
    11401 gcagcaaagt aaatatacca ttttccattg atataatgga cttcaggtgc ccagatcaat
    11461 tcactcatcg gtccttcttc atgagcctgc catacaatca ggcttgcagc atgttctaat
    11521 tcattcagtg actttgccct tcttaattca atcacttggt agcctggaac cgatcctgta
    11581 aaataataat aaccatctgt atgtttatat acccaaggat ctgcccgttg ccgaacgata
    11641 ggattattat aaagtgtctt catcataaca aatttcctca cattttcttt attctaatta
    11701 cattccaaga gtatttttct atccctacat ttaattcgtc atgtttttct attgaaaaat
    11761 agtttatttc aggttttatt ttttctggtt cttcacgtgt atttttgtcc aacagatcgc
    11821 tactggttaa tgtgtagtgc tctccttgat ctagcaaatt gaaatcatgt tcaatcgtta
    11881 tcttttgttg ttcatttgtg cgattgatca agaaaatagt cagctgattg ccgttatcta
    11941 cgatcacact gtccacataa ggaacatttt ttatcttttc acaatcataa acagggcaaa
    12001 tgatttgttg tttcaatact tgacctttag catatttcga taagtgttgc atcacataat
    12061 aagttggatt cctccatgct tgtcctttag catctgtcag tattaaaggt attgtgttta
    12121 ctaacaatga ttgacaacta atctttacgc gatctgcgtg tcgtaaaata gccagcccta
    12181 atgaaccaaa taataatgta tcttccatag taaagtaaca ccaatcgatg ggactaccga
    12241 cttcaaaatc cctatgtctt ttttccggtt gggcatgtac attccattca tcaaacgcta
    12301 gatacattgt tttctcactt ctcaaatatc cttttacata atcacaagta gcaacgattt
    12361 cttcaatttg acgatcgaat cgataagctt ttgcaaggta tgtcgctgta tcatcacgct
    12421 catttttagg aggtttagtc aagtcttctt catcatactt atcaatatag ttatgcaaag
    12481 ctaaataatc tacattctca taagcttctt ctaatgcgat acgatcccat tcagggtacg
    12541 atggtaaacg tggcgtagag cttccaacta agattgtttc aattgaatca tccactaact
    12601 tcatgacttt tgaggcttca tttgctaacc taccatattc ataagcactc ttagccccca
    12661 cttgccatgg accgtctagc tcattcccta aacaccataa tttaacgcca aaaggctttt
    12721 tttgcccatt ttttattctt aaatcactcc agaaggttcc ttttggaaaa ttacaatatt
    12781 ctactaaatt ttttgcttca tcaattcctt ttgttcctaa attcaccgtg aatatcggtt
    12841 tcgcttgtac atgactgatc cattaaaaaa attcgtggag tccaaaagaa tttggttcta
    12901 tacctcgcca tgctaaatca attttttttg gtcgttgttc tttcctacct acagtatcca
    12961 cccaatcata accagatgta aagtttcctc caggatagcg aatgacacct aattctaatt
    13021 cctttacata agagaggaca tcttgtctaa acccgtcacc atcagctgtt tcatggcccg
    13081 gttcataaat ccctgtatag acgacacttc ccatatgctc aacaaatgag ccaaacagcc
    13141 gagaactgat ctctcctacc gtaaaatcct ctctgatttt tactttcact tcttcgtcca
    13201 acagctttcc ctacctttca tttcctatag tcaagataat agatacaagt ttccatacgc
    13261 caccagcaaa gataaaaata aaaggaggga aaagaaatat gagcagtaca aatcctaata
    13321 acaatacgca acaaagaata tttctcaaaa aatcaaaaaa tactttccat gcactttgtt
    13381 caaaaatttg taaaaatgtg atatctttgt tttcagaaat cacatatatc caggataaaa
    13441 gaaacatgga ataaataagt aaaactacat atccactaat tgaaagtacg attgtctgtt
    13501 cttctaataa ttgattaaca agaaaataaa taatgatcgt taacagacta aggaacaagt
    13561 tcttaccaaa atgttgcttt attccttgaa agaaaaaata aatgatattt cctgatccct
    13621 cacctttcca ttgcgccatt acataaaaaa cagcacatgt agcaggcacg atagtaataa
    13681 caggcaaaca cacgattacc cagagaatac ctaataagat gtaatcagaa accacaatca
    13741 actgtccata aaatttagtc tcaactattt tcatcttcct cactctttca ttccagacat
    13801 agctgttcct tgaacaaaat atttctgtgc aaaaatataa accactagta atggcagcat
    13861 tgaaataacc gttccagcca ttacaggtcc ataaaaagaa atgttctgcc ctgtaaataa
    13921 agctaaccct gctggtaaag ttctcatttt tgaactactt gtcaatatca atggataaag
    13981 caagtcattc caactattca tcagcgtgaa aatccctaat gataaaagag taggtttgct
    14041 caatggcaac atgattttca aaaaaatatt aaactcactt aatccatcaa tgcgtgctgc
    14101 ctcttccaaa gaatctggta aagtaataaa aaaggaacgc atcatgaaga taccaaatgc
    14161 agttgtcata cgaggaatga tcaaacctgc ataagtatca agcatgccaa gcaaattgac
    14221 ttcaataaat agaggaatca tgaagacttg gaatgggatc atcattgtca gcaaaacgaa
    14281 atagaacaaa actgattttc ctttgaaacg catacgagca aatgcatacc ccgcaagaga
    14341 atcaaagaat acagaagtga tcactgtccc tccagcaaaa atcactgtat ttttgatgta
    14401 atctaataaa gggatcgttg tccaaacctt aatatagtta tctagggtat agctatctgc
    14461 aaatattcta ggcggaaatg agataatgtc tttttcgtgt ttaaaagaag aaataatcag
    14521 ccacaaaaaa ggaaacgcaa taaccaacaa tataactagc atactaatcg ttcgaatgat
    14581 ttttttacca ctaactgctt ttcgactatt cacagtaact gtaatcgagc tttctggtgc
    14641 catacaggtt tccctcctat ttcatttgtt tttctttccg tgacatgtac acattagtgg
    14701 agatagctat agttgcaaca atgataaaca aaaccgaaga cagtgctgat gcatagccca
    14761 attcataagg tgctgtaaat cctcgactgt aaatatattg aacagctgtt tctgtcttaa
    14821 attgtgggcc accagcggtt gcaacatata cttggtcaaa cacttgcatc gaaccaatca
    14881 gattcgttaa aatacaaaaa cctaaggagg gaacgatctg aggtagcgta atgttaaaaa
    14941 actgctgtcg atttgttgca ccatccattt gagcagattc atacaaagag ggagagattc
    15001 cctgaatagc agtcaagaga atcgtcatag taacaccaaa attcttccaa acagtcatga
    15061 tggctaccgt cggcatagca agggttggat cgcgaaaaaa ttgagggatc ggcaagccga
    15121 cttgtgaaaa caagtaagga atcgccccta cattcggatc aagcaacata gaaaaaataa
    15181 tcgcaattga cgtcaatgaa caaataactg gaatgtaaaa tgtcgaacga caaaaacgat
    15241 tgaatctggt atttttagat aatgctactg ctgctaataa accaacgata atttgtgttg
    15301 gcgtttcgag tacggtgaaa tataaagaat gaatcaacga attaactgct cgttcatcac
    15361 taaatatacg tgtgaaattt tcaaaactta caaaatttct tgatgtaaag aaaatattca
    15421 aatctgtaaa actaatagca aatgttccta aaagcggtat gacagtaaat aataataaaa
    15481 ctaaaaaaga tggagttaaa aacaaatacg cagcacgatc aggacgattc caatatttgg
    15541 tgaatccgtt catatagtcc acctcatctt tcattttgat aaaatataca atcttggttt
    15601 aaacgaatct gaataagata tccgaacaat cgctgtgatt tttgcaataa cacctatctg
    15661 caaatactag gtcgatcatt cagatatctg tatcttgcca acagtctatc ctgtataaag
    15721 ttctgtacta tttttgcagt aattgattga tggcatcatc tgcatcgttc atgagtgtct
    15781 caggatcttc tcctgctaat aatttttcaa tcagtggatt gatgatatcg ttattgatcg
    15841 cagagatttg ggtaaaacct ggtaaatatg gctctgcata gtccatttgt ttcgataatt
    15901 cagatacaat aggatcattt tttacttctt catcttttgc tactgaatcc aagtaggcag
    15961 gaaaaccatt ttcgatactc cattttttac caatttcagt tgtattccaa tatttcaaaa
    16021 actcatagac agcttctttt ttattattat ctgtacttgc cggaatggca tatcctacgc
    16081 catctaaaat agctgttttt ttgtctgaat cactcccttg tggtaaggta gtcacattat
    16141 aatcaatctc gtttgctttc aatccgttat ttaaccatgg cccattgatt tcaatggcta
    16201 gttgaccagc attcattaaa ttgtccattt cggcgccaga aatcgattct ggaccagtct
    16261 tgtctacatg gatcattttt tggatcgctt ttaacgtttt aaggttctca ggagacgcaa
    16321 attcagactt agtataatca tcgttcacta atttaccgcc atttgctaac atccagttat
    16381 ataacggtgc agttccatcc ttattgaaga caaaacctga tacattttta ctactatcag
    16441 ttaattttgg tgccatttct gtcaattctt cccaagtagt aggtggttgt tcagtatcta
    16501 atccagcttg tttaaacaaa tcattattcc aatacatata catgccttgt acctgcatgg
    16561 ggacaaaata ctgtttacct tctacttcac ctaattcaat agccgtttct ttaaaatcag
    16621 atttatctac tccatcatat tcccagaaat cgtctaatgg ttgtaccgct ccgttttcta
    16681 catactgtgc aaaatctgga taatttaatg caataaagtc aggtgctttt ttagaagaaa
    16741 tcgctgtagg aagtttttca ttcaaattgg cccaagtcat gatatccatt tcaattttga
    16801 tgtttttatc atttgtttta ttaaattcat taacgatttc ttgcaatatt tcaccatcag
    16861 atgcagtaaa cccattccaa aaagtcaaag tcacttcttt atcagaatca gattctgctg
    16921 aacttccgcc accgcaagct cctaaaatac ctgttgcagc taccaacaaa gagccaatta
    16981 accatttttt acctttcatt ttttgttcct ccttcatctg ataaacgaca tctgaccaca
    17041 ttccaagata gcggtttcaa taaacaattg attttcccat cttcagcttt tacatttgtt
    17101 gaatgacttg gttttacttg ttcactttta ggaagattag ttgcttttgt atcaaatcca
    17161 ctcatttcag aaaaatcaat cactccctct aatgataatt cagacaagtg aacagttaga
    17221 ttcatttctt cttctccacg gttaactgca aatatgacaa gttctttgtc atttattacg
    17281 gctatacttt ctaaatacgg cacttcttca aaatcttccg tcgcatagtt tgctgaacga
    17341 atttgtgggc ttaacacctc tccttgtcca tacacagcag cttgcataaa tggataatag
    17401 atcgtctgtc tccaagcttc tccgtttgtt tctgtcctga ttggtgcaat aacgtttacc
    17461 aattgagcaa gacaagcaat ctttactcta tctgcatgtt tcaataaagt gattaataga
    17521 cagcctaata gtaatgcatc ttcaaaatta tagttatctt ctagtaaagg aggagcaatc
    17581 tgccaaggcg ctgcttgctc atcttgttcc gctgaatgat accaaatatt ccattcatca
    17641 aaggacaggt tgatctgctt cttactatgt ttcttcgcct tgactgtatc gcacatcgct
    17701 gtcacttcag aaatgaaccg atccatgtct aaggaacgtg caagataatg agaaagatca
    17761 tttttttggt tgccataata ttgatgcaga gacaaataat ctatttgatc gtacgcttga
    17821 tcaagaactg ttaactccca atcaccaaat gtaggcattt gccgattcga acttccgcat
    17881 aaaaccacct caattgaatc atctacgagt ttcatcgcct tagccgtttc ctctgctaac
    17941 cgtccatatt cttccgctgt tttatgtcca atctgccaag gaccgtccat ttcattccct
    18001 aaacaccatg tcttaattcc aaaagggctc tcttgaccat tcgaacgcct taaatcactc
    18061 caataagttc ctttaggaaa attacaatat tcgactaaat tacgagctgc atctactcca
    18121 cgagtaccca gattgacagc catattgatt tcggccccca catgctgaca ccagttataa
    18181 aattcatgaa tcccaacttc attcgtttca attgtccgcc aagctaaatc caaccttcta
    18241 ggtctatttg ctatgggccc aatactgtct tcccaacgaa atcctgacac aaaatttcca
    18301 ccaggatatc taatcatggg aatgttcaat tcttttacca agtgaatgac gtcttggcga
    18361 aatccacttt tattagctga tggatgatct ggctgataaa tcccttcata tactgctcgt
    18421 cccatatgtt caataaatga actgtacaac cgcggatcaa ctttgcctac acgcttgaca
    18481 ctactaatca acattgtggc ttccataact gacctccttt aatttataca tgaataatac
    18541 acctcgttga aagcgttgtc aataatataa atgttaaact tacttatttt ttatcgattt
    18601 taattaacca aaatattaat aatgattaaa acaaataatt taatataact tttattaaca
    18661 aatatgttaa tatataagaa aaacctttta agaggtgata tatatgcaac tacaaataaa
    18721 cgaagaatcc ttaccggtat atgaagcatt agctagtaaa acaaggatca agatcatcca
    18781 gctactttcc aaaaaaaaga tgaatgtcaa agatttggca aaagaacttg gagtgagcag
    18841 tgctatcacg acgatgcacg tgaaaaagct agaggaagca aacatcatca aaacagaaaa
    18901 agtaggacaa caaaaaattt cgagccttcg tgtcgataaa atcgatatta gttttccaga
    18961 aaaaatattc aatgcttttg atacgaaaga aacatcgata cctattggtc actatacaaa
    19021 ttatgcaatt gaaccaacat gcggtttagc tacgatccat gattttatcg ggaaagtgga
    19081 tgaaccacgc tattttatgg atcctcgaag gatggatgcc cgcattctct ggtttaccag
    19141 cggatttgtc gagtatcagg cacctaattt tttaaataca gaggatacat tggagatgtt
    19201 ggaagtttct gtggaaattt cttctgaatt tcctttttca aatgacaatt ggccttcaga
    19261 tatcactttc agcctaaatg gtgtggagct aggaacatgg acgagccctg gagattttgc
    19321 tgatatccgc ggtaaatata cgcctgattg gtatccagat aatcttaatc aatacggctt
    19381 gctgaagacg atccgaatta ccaaacactt gacgaatatg aatggagaac ccttgtcaaa
    19441 catcacgatc aacgacatcc ctaaagagca agacacttgg catttgcgaa tcgaagtgaa
    19501 agacgatgcc aaacatgtgg ggggctgtac tttatttggt aaaggattcg gtaattacga
    19561 tcaggatatc aaattaaaag tttactacag ctgatatttt tcttttctat tttataaaac
    19621 aaaaacagcc aatccctatg aagggacggc tgtttttgct ttattgttat ttccagaaat
    19681 catccaaaac ggaaattggc aaatgtcgtt tatgttgtgt cttgttccac catgcttcga
    19741 ttttttcttg tgcttgagcg ggaacttctt ttccttctag ataatcatca atttcattat
    19801 atgtgacacc tagtgcgact tcatctgcaa tcattggttt cccatcttca agatctgcag
    19861 tcggaatttt tgtgtataat ttttccggtg cattcaatgc ttgaagcaat tgtttccctt
    19921 gtcgtttgtc caaacggaat aatggcaaaa tgtcggctcc accatcacca aatttcgtga
    19981 agaatccagt aatgttttct gcagcatgat ctgtccctaa tacggctcct gctctttctc
    20041 cagctacggc atattgcgta atcatacgtt gacgtgcttt gatatttcct ttgttaaaat
    20101 cgctgattcc cacacctgct tcgcttaata cttgtacttg tgcatcaaca gctggtttga
    20161 tatttactcg cagactaaca tcgggctgga taaagtcaag agcagctttt gcatcttctt
    20221 catctgcttg ttcaccataa ggaagacgaa cagcgataaa ttgatagttt tcatcttttg
    20281 tttcttcacg catttcttcc atcgttagtt gcgccaaacg gcctgctaaa gtagaatctt
    20341 ggccgccgct gatacctagt acaaaagttt taagaaacgg atattttttc aaatatgctt
    20401 tcataaagtc aatactttta cgtatttctt cttttggatc aattgttggt ttgacaccta
    20461 attcattgat aatttctgtc tgcaggctca tttattctcc tcctatgctt ctgtatgaag
    20521 ttctgctacg ctttttttca ttcgttccat gatagccatt ttatggttcc agcattcagt
    20581 agacagatcg actgggtatt tttgcggatt caagtctcgt ttatactctt cccatagtga
    20641 atctagattt tcttttgtat attctttgat ttcgtttagt gcaggtaatt cgtaaactct
    20701 ttttccttcg ataaagatat cttgtaaaac tgggcgagct gtaaagtctc gaacagtttt
    20761 attgataaac gtatgcactg gatggaacat gaagatagct tcttcttctc gagggtcttc
    20821 atcccataat gtcacgtagt ctccttcaga tttcccatca aaatttcgag tgatgcgcca
    20881 cacttgtttt ttccctggag tagttacttt ttctgcattg cttgataact tgatcgtatc
    20941 catcattttt ccttcatcat cttcgatcga tactagcttg aatactgcac caagggctgg
    21001 ctgatcatag gctgtgatca attttgtccc aacaccccat acatcaattt tagctttttg
    21061 catctttaaa ttcaaaatcg ttgcttcatc caagtcattc gaagcataga tttttgcttc
    21121 cgtaaatccg gctgcatcta attgttcccg aacacgctta gatatatagg ccatatctcc
    21181 actgtcgatg cgtacgcctt ggaagttgat tttgtcacct aattctttcg ccacgcgaat
    21241 cgcacttggc acacctgatt tcaacgtatc ataagtgtcg actaagaaca cgcagtcttt
    21301 atgggtttta gcgtaggcca taaatgcttc ataatcattt ccataagatt ggactaacga
    21361 atgagcatgg gtcccgctag ctggtatccc aaatatcttc ccagcacgta cattgcttgt
    21421 tgcatctgct ccgccgatat atgccgctct agttccccag actgctgcat ccagttcttg
    21481 tgcccgtcta gttccaaatt ccaatagtgg atcttctccg atgacagatt tgatacgagc
    21541 tgcttttgtc gcgatcaacg tttggaaatt gaccatattc aacagtgctg tttctactaa
    21601 ttggcagtgc gctaaaggcc cttccacttg gatcaatggt tcatttgcaa aaactagttc
    21661 tccttcacga gcagaacgaa ctgttgcttt aaattcaaaa ttttgtaaat actccaaaaa
    21721 accttcagga tagacttcta atccacgcaa gtaagcaata tcggaatcat tgaacgtcag
    21781 attctctaaa taattgacta accgttccaa tcctgcaaaa accgcgtatc cgtggttaaa
    21841 aggcatttct cgaaagtaac attcaaaaac agcatgtaaa tcagctcgac ctaattccca
    21901 gtatgtctgc atcatattga tttgatatag atccgtatgc agcgtcaaac tgtcgtctgg
    21961 gtaagtatta ttcatcttgt attactcctc ttggcaagtt ttcatttcct acccattata
    22021 acaaaaaaaa ttaataggaa aagaaatctt ccattttttc tgaaaaagca catgttttac
    22081 ggatatttct tgctaaaaat gaactccatc tgctaaaact atcctttctt attggtattt
    22141 aacctaaacg agaaagaata cttattttat tcaatgtatc cgagacacga agaaattagc
    22201 gttttctatt tttcttactt gataaaataa gtagattttc ctactacaat cgtagccgtt
    22261 aagataccga tgattgaaca aatataacca aatgtctcta caacataatc aatggcaaaa
    22321 gtttttgaag caataatacg tctttccttt tttgcttctt atccccaaag aaacggatat
    22381 aacttattat gtattgaatt tttttggaaa ggcgaattat gacaagatat accttgcgtt
    22441 gaaaaaactc gcggaaaaaa tatcacacgt ttttaaaaag atacaaagta agaaagcagc
    22501 tgtcaataga atggaaacaa aaatggcgag acatcaatca gtctcgcctt gttttttatt
    22561 gtatacatta gatatggagc aggttaccat aaagatgatt attagtcgtt attaaataga
    22621 atccttcgta tttttttgtt ctatcactac aatatcttta tttggacgtt tgaattgtat
    22681 tccggcacgt tctagctcct gtttcactcc tacatggact ttgacatctt ctcgtgtcat
    22741 ttttaaaacg tgatgaatcg catagtcgat ttcttgcctt gtgatttttt tcttcgtatt
    22801 gatcatcacg acttgctttt tatcatatgt gtctaccaat agcagttgtg ttcgatctaa
    22861 cgaaaataat ttgtaataat taatagccgt attcccaaaa aagaattcca ctactttagg
    22921 ataaaatgtc ttaatattta aattactatt cacaggtgtt tcgattagct tcatctcatt
    22981 cctcttttct atatagtcga tttagatcca atcttaagtt atgttaccat ctttcctagc
    23041 attaaggaat cggttacaat tgaaccttta aagacaaaag gatttacctt ttatcactta
    23101 atagtataac ataatttcat gagaaaaaaa tacaattttt tcttgaccat ctcgttacgt
    23161 aaaggattat gcttacattt gtcaggagga aaaataatgg agtacacaat aaagaaaatg
    23221 tcagagatat caggagtcag tccgagaacc ttgcgctttt atgatgagat tggactgcta
    23281 aaaccagcta ggatcaattc ttcaggttat cggatttatg gcaaaaaaga agtcgatcgt
    23341 ctgcaacaca tcttatttta tcgaacaatg gcattcaagt tggatgatat ccaagaggtc
    23401 ttagacaatc catcttttga ccaccagaaa gctttaatca agcatcagca aatgttgctg
    23461 gaaaaacggg cacaaatcga taccctcttg acaactgtcc aacagacact tgatatgtac
    23521 gaaggaggaa gaaaaatgag tgacaaagaa aaatttgaag ggtttaaaca acaaaagttg
    23581 aaagaaaatg aagaatccta tggacaagag attcgacaaa aatacggaaa aaaaaaggtt
    23641 acagcatcca atgaaaaatg gatgaatatg gataaagaaa cgtatgaaga aatgcaaact
    23701 gttgaaaagc agatgttatt tgatctcact ctttacttaa aagaaccaga agatgaaagc
    23761 ttagcagacc gtatcttcca agctcataag aagtggttat cttacagctg gcctgaatat
    23821 cagccaaaag cgcataaagg actaggaatg atgtacgaag ccgatgaacg gttcactgca
    23881 tattatgacg agcgaagtgg tcatggtgca gcaaaagctt taaatgaaat tatccaaaaa
    23941 aaggcgtaat cttttacatg atccaacaaa aaagtacacg taaaatgacc taataacaaa
    24001 acagcagaca cccaagttag atttttatgc taacttttgg tgtctgctcc attctttcac
    24061 aagttttttc atcctacttt aaatatattc atcgttgaat aaacgtataa ggtattttca
    24121 ctttatcctt tttttctgta agaaacaatt gaaatgccat ttctccgcaa gcttccagat
    24181 ggtggtcgat ggttgggaaa ccaagaagct cgctgatcag caaattatcg cgtccaatca
    24241 ccactggtgt cttatcttgc agatagcttt gcaagatgcc tgctgcaacc atatccgaat
    24301 ttgtaaagac accatcaact ccaagctctt taaaaaagaa gcctgcttgt tttcctttct
    24361 ctgcatctat acaatcccaa aaaacaagtg attcatcaaa agatggaaaa agttgatgac
    24421 atagctgcaa agtgattttt gagttataac ttagcctgcc acttcgcccc aacgtgatgc
    24481 ccaattgctt tataccttgc tgcttcaaat aagtaagacc ttctgtaatt gattgttctc
    24541 gatcaataaa gacacagctt gcctgttttc ccttgatttc ttcacaaaaa actagaggtc
    24601 catatttttg atatgccaat aaatcatcta gtgtattggc acgagacgta atgatcaatc
    24661 catcgaaagc tttagctgca aattcttcaa gataccttcg ctctttttct ttctgatagc
    24721 ccgtcggtaa aagtgtcact ttgtaatcat aatcaaacgc tgcttcaatg atcccactga
    24781 tcaactgatc aaaatacgga tgattggtat aaggcagaat cactccaatg cttttcgttt
    24841 ttccataact taactgtctg gcatttgcat tagggatata atcgacttct ttcatgaccg
    24901 caagtatttc tttccttttt tcctcatcta catatggatg tccattgatt acacgtgaaa
    24961 tagtagaaac tgaataacct gtgagctttg cgatatctcg aatgtttgcc actgctgctt
    25021 cgccacctat acaaaaagat tttcttgtaa atagtctacc acagctggac cgcattcaaa
    25081 aaactgatac atttttcctt catgcgaaaa actgatccgc tgcccgcccc aatatatatc
    25141 ttttgtcaaa ttttcgattt cattcattgg tatttctttt actatattcc tattcaaccc
    25201 ttgaaaactg tacaacgtta atacggatcc cttgaactcg acaaacgcat aaaaataatt
    25261 gttcgttgtg ccatctgtta tctctacata agtattcatt gcaagccctc cttttgatac
    25321 tttgagtata gtgtatgaaa cgcgtctcat agcaaggagt ttgttttgcc aaagaaaagt
    25381 tttattcctc tttcattatt accaaagtct cttattttaa attgaataca ttataatgat
    25441 actgtaaata tatctttatt attagtgaat ggatatttac ttttaataga taaaatatta
    25501 taactattaa aaataactat tttcattata attacacaca aaaaagagcg tgagacaaaa
    25561 gtaaagaaca cttttgtctc acgctctaaa cacgtataaa cagcggaagc agaagcaact
    25621 ccttcggaaa taagctgaaa ttcacaaaaa tttgaaaagc aatttccgtg aatttcttct
    25681 tatttcttgg agttaaacgc ttctgttcca gcctcttttt atctgtcttt cactaatgat
    25741 cagttgtaga taagcaagtt gctttttctt acatcatacc gcccatcatt gatggatcca
    25801 ttgcaggagc tgctggcgct gctggttctg gtttatctgc aacgactgct tctgtcgtta
    25861 ataacaaggc agaaacggaa gcggcatttt gcaaggcaga acgtgttact ttagttgggt
    25921 ctacgatacc tgcttcaacc atgtttaccc attctccagt agcagcattg aaaccagtgc
    25981 ctagttccac gttcttcaat ttatcaacaa tcactgatcc ttcataacca gcgttttcag
    26041 cgatttgacg aattggttct tctaaagcac gaacaacgat cttgatacct gttgccacgt
    26101 ctccttctgc ttctacagca gaaactttgc tgataacgtt cactagagct gtaccaccac
    26161 ctgagaccat tccttcttct actgcggcac gtgtagcatt caatgcatct tcaatgcgta
    26221 attttaattc tttcaattct gtttcagtag ctgcaccaac tttaacaaca gctacaccac
    26281 ctgctaattt agctaagcgt tcttgtaatt tttcgcggtc aaaatcagaa gttgtttcag
    26341 caatttggtt tttgatcaat tgcacacgag cttcgattgc ttctttttct ccagaacctt
    26401 ctacgatcgt tgtattgtct ttatctacaa caactttaga agcatttcct aagttttcga
    26461 ttgttgcatc tttcaattct aaacctagat cgtcagtaat tactgtacca cctgtcaaaa
    26521 tagcgatatc ttcaagcatt gctttacgac gatcgccgaa accaggagct tttacagcta
    26581 ctacgttgaa tgttccacgg attttgttca atacaagagt tggcaatgct tcaccatcta
    26641 catcgtcagc aataatcaac aatggacgtg attgttgcaa gatttgttct aacaatggca
    26701 gaatatcttg gatgttagag atttttttgt ctgtgatcaa gatatatggg ttttctagaa
    26761 cagcttccat tttgtcgtta tctgttacca tatattgtga taagtaacca cgatcaaact
    26821 gcataccttc tacaacatct agttctgttt cgatcccttt tgattcttcg attgtgatga
    26881 caccgtcgtt tccaactttt tccattgcat cagcaatcaa atgtccaact ttatcagaac
    26941 ccgaagaaac agctgctact tgtgcaatgg cttcttttga atcaacaaca gttgagatat
    27001 tatgcaattc ttcgactgct gcttttgttg ccaattcgat cccgcgacga atgcctagag
    27061 gattagcacc tgcagttacg ttttttaaac cttcgcgaac gattgcttgt gtcagaacag
    27121 ttgctgtcgt tgtaccgtct cctgcaatat catttgtttt tgatgctact tctgatacaa
    27181 gttttgcacc catgttttca aaatgatctt ctaattcgat ttcttttgcg atcgtcacac
    27241 catcatttgt gatcaatgga gagccgtaag atttttctag tacgacattt cggcctttag
    27301 gtcctaatgt tactttcact gtatctgcta atttatctac cccgcgtaac atagctgcgc
    27361 gtgcgtcttc tgcaaatttc aattcttttg ccatgattga ttcacctcat tgattatttt
    27421 tgattatttt tttatcttcc aaatttactc aacaatcgcc atgatatctt ttcctgcgac
    27481 gatcagatat tcttttcctt cgtattttac ttctgtaccg gcgtattttt cgaacataac
    27541 ttggtcgcct gcttttacag cggcaggaac tttttcaccg ttttctaaca aacggccttc
    27601 accgactgca acaactgtcc cagtttgggg tttctcttta gcagcagaag ctaaaacgat
    27661 tcctccgaca gttttttctt cttcttttgc gacttcgata atgacgcgat cacctaatgg
    27721 ttttaacacg ataaatccct ccgataactc tttttttaat ctgttagcac tcttaacctt
    27781 cgagtgctaa cctacatttt taataatact cattctccct cttttttgca agcttttaaa
    27841 tcaaagaaaa gaaattttat tctgacagct gcttattgaa attatgatat aattgaaatc
    27901 aatcgatata agcttgcctt gcatgcttgt tatcccttaa aaggaggaat tacatgtctt
    27961 taaaaaaata tagcttcctc agcatttttt gctatggtct aatttttatc tcacccttga
    28021 tcctagcttc gataggactt gtaaaatcta cgtctgaatt gatcacagca actgcagtca
    28081 tgtatatact aggcgccgtt gtcctagcta ttttttattt taagcaacgt gaacccttag
    28141 cgatcgaatc agcggtaaaa aaagcttctg ctccaaagat tgttatttat ggattggttg
    28201 gaatctttgt cgcattgatt ctgcaaagca tcgctgtcat gttagaatct gtattgtttg
    28261 gcaaagcaac cccttccgag aatacacaaa atatcatcca gatgatcatg gaagcacccg
    28321 cttttatttt ggcgacaact gttgctggac ccatcatgga agagttcgta ttccgccgca
    28381 gtatactggg gatcatcagt cgatattcaa atttttgggt cggagcagtg atcagctctt
    28441 tgctgtttgc gtttgctcat aatgatggtc atctgctaat ctactttttc ctaggtttct
    28501 tcttcagcct agagtacaaa gcaactggtc ggatctggac atcgatgatc acacacgtag
    28561 gaatgaacac attagttgtt cttgttcaat tagctgtcca aaaagggcta gtataaacat
    28621 gactataaat actcctgtca tcctagattg gtgattttcc caatctaaag cgacaggagt
    28681 atttttgttt tttagtcgtt catcttttct gctgctccat cattttttgt ctgtctttcc
    28741 tttttgcagt ttgcagaatc tatttttttc aaaaatggga aataaatgaa aaaactaatt
    28801 gacaacacga taccttgtaa aatcgctgca ggcactccgc ctactaagaa acccgagagg
    28861 atcggcggtg ttgtccaagg gacttggact gctgtaaata aaggaacgaa tccaaaatat
    28921 aaagcggaat aggtaatcaa tcctgaaacg accggtgcga aaataaaagg aacagccatc
    28981 atcgggttca tcacgatcgg tgtggaaaaa ataattgatt cattgatgtt gaaaaaggct
    29041 ggtaaaagag caagatttcc taattctctg tatttcttcg atttcgcaaa aaccgtcata
    29101 aaaaatacag cccctaacgt caatccagca cccgtaaccg tcatgaactg atcgagaaat
    29161 tgctgagtaa cgatatgtcc gccatttgcc accgtcaact cttgtcctga ggctaagatt
    29221 tctgcatttg ctaatgaatt cgcttgcaag acgggactaa ccaacccatt gaccatcgca
    29281 gatccatgaa gaccaaataa ccataaaaac ggaacaaaaa aacctaataa aagcacacca
    29341 aacaacgtgt ctatgaacga ttgaatggga agctggacat atgtccatac tccttgaaca
    29401 acagtcattc tgaacaaatg ttgaaaaacc gctgaaccga taagcgtaaa agtgactata
    29461 acaatgattg gaatcaatga agtaaaagac tgccaaacat tttcaggtat cttctctctt
    29521 tgcttccacc ctttcgcaag tcttactaaa cgtgcatagc cccatccgat gatgagccca
    29581 gttgtaactg ctcctagtat cccttcagca cctgtccaga cagtatctat ctgagtattt
    29641 agtggctgaa taagcagtag ataagcagac aaggctgtta tgccgactgg taagcgatcg
    29701 atgtcttctt cttttgcata agcgtatgca atggcaaaaa cgatccaaaa ggctatcaca
    29761 tcaaatgtag cctggtatac ttgtgtaaaa tacatagaaa atcctgtttt gtctatccac
    29821 tgagcgatgt aatccacagg aaaatgagca aataagaaaa acagcgagcc aacaataatt
    29881 attgggatcg tgtaagtcat tccattacgc aaagcagtta agggtctaag attcacaaca
    29941 gatgttatca ttggtaaaaa ggcacgatct aaccactctt tcatttctta tctccttcaa
    30001 cttctatttt tatcaatcag ttttcttaat ttaactatac ccaatgatca cgaaccatga
    30061 aaggaatgcg cactgagctt ttcaaaaatc ggattctttg acgaatattc agcaactgct
    30121 tacaatggac aggaataaaa aaggaggttt tttatgaaaa ttgcactcat tggagctact
    30181 ggccatgcag gttcatttat tttagacgaa gcattgtcca gagatttaga cgtgacggca
    30241 atcgtgcgtc atccagaaaa attgccatca gatgttccgt ttatacaaaa ggatttattt
    30301 gagttgacaa ctactgatct tgcatctttt gatgttgtcg ttgatgcttt caacgctccc
    30361 aaaggaaaag aagaaatgca tcaaacttct ttgtcccatt tgattgagat gcttgaagga
    30421 accgatacac gcttaattgt cgttgggggt gcctcttcgt tattcttaga tacagaaaag
    30481 acgactcgta tgattgacca agtacctaaa aatgcaccct tctatccaac agctttcaat
    30541 atgtacgaag gattgttaaa attgaaacaa tctaaaaatc tacattggac ttatatcagt
    30601 ccagcttctt tatttgatcc acatggcaaa cgtacaggta cttatttgtt aagtgatgat
    30661 tatttacaaa gaaacgctgc tagcgacagt acgatcagca tggcagatta cgcgattgca
    30721 ttagtagatg agatactgga tgagaaacat gaaaagcagc acatcagtgt agtcagtcga
    30781 taatacggtt atttttttaa tatgctaaaa aaataaaaat agaagataaa accaaccctg
    30841 attgatttta tcttctgctt tttatatttt tattgattga actgtttaaa tcgaaaatag
    30901 ctcagttgct tcatctggat ctgtatcata gatttcaaaa tcatgtccaa cgccaaaatc
    30961 tttttctttc aaaacatttt ccattgttaa atgagcaagt tctttcatat tgttttcctt
    31021 gctaagatgg ccaagataga ttcgtttggt ccgattaccg atcacatcag tcatgacaag
    31081 tgcaccgtct tcgttggaaa gatgtcctct atctcctaaa atacgctgtt ttaaattcca
    31141 cggataaggt cccatgcgta acatctcaag atcatgatta ctttccatca aataaccatc
    31201 ggcattttca atgacacctc gcatataatc actgcaatat ccggtgtccg tcaacatgac
    31261 gaatgaccga ttttctttat ggaaacgata aaattgcgga gccgctgcgt catgggagac
    31321 gccgaaactc tcaatatcga tatcgccgaa tgttctgact ttccccatct caaatagatg
    31381 tttttgttcc actggtacat ttccaatcag tggttccatc gcttcccaag tctgctgatt
    31441 ggcaaaaaca tccaaatgat acttacgagc caagacaccc acgccatgta tatgatctcg
    31501 atgttcatgc gtcacaagta ttccatctaa ttggtccggt gtccgatcaa tttgagccat
    31561 caaggaagtg atttttttac cgctaagtcc tgcatcgacc aaaagccgtt ttttttcact
    31621 ttcgatataa agtgaattac ccgaacttcc acttgctagg atactgattt taaaagctgg
    31681 tttttcttca gccatttgac tcctacttcc cttcttactt tgtaaacttt tttcattata
    31741 ctcgcttttt tattgatctt ccacttttgg tacagtattg tttgtcacga tcgtattgct
    31801 gaaagcgttg acgctctcaa tttggaccac tttatcgctt gtttcgatcg ctacatgcca
    31861 aacaggaaca tacacatttt tttcccggac tttcagaata caggaataag ctagctgacg
    31921 ccataaaatt tttgatcctc ttggtatttt gttattgatg tacaatgtat tgattgcttc
    31981 ctcttccgta tacagttccg tcttttcgcg cagctgctcg atatcagata aatgcgtctg
    32041 catgtatttc gtgatgctat aatcattatc gtttttttct aaagaaagaa cgatttttgc
    32101 tgtatcatca ttgaatggga tgccttcata ctcttggctt gcttggattt ctagtggttc
    32161 ttcttctaaa tgagaaaagt ttttcgtgta cacataatca ttaccgaata aaatattgtc
    32221 tttgttagat aaaaaatctt ccaaggaact actaatttta tctggatcga tcacgttctt
    32281 ttcagcatct gaactggata aagatttcgt caaaacatca ccgtctaaaa acacaccact
    32341 tgccagcaag ccgcttttac cagccttgcg ataattcgct aatgaagtgc taagattggt
    32401 ctgctccgcg ctaagataat agccttgctt gttctcagac gaaatcttcc ccgtgtaagt
    32461 gatatcgtcg ctagccagac gttgttggat tgattcagtc tgattagagc gaatgacatt
    32521 gttttcttgg tataccgcgt catgataact gctgaataaa aacacattca accctaaaaa
    32581 agcgacgaaa aatatccatt cgattttttt gaaatccaat taattcgcct ccaatccagg
    32641 taatttttcc agaaggtcgt tagctgaata ccagttatct ttatatttca cataccattc
    32701 aggtaagaga tcaacgacac gattcgtttc tttgacattt tgccatgtat agccaacgat
    32761 cactgaacca atcttgttgc tatctgctcc cttagctttt aactgctgga tgatttcatt
    32821 cgtacttgca agttctactt cttcgtctga aggtatcggc acttgaatcg tattcaaact
    32881 ggtttggata ttcacttgca cagaaccatt cgtttcaccg ccaacttcca caccaatctg
    32941 tcctctgctg tctgtcccaa aaaccgggaa gccttccacg aatatccgat aatcgatcgt
    33001 attgtcactg cggtcaaaat atctgaggtt ccctaaagaa ctgcctaatt tgctaatata
    33061 ggggaaactt tcagaaaata cactagcatt ttcttttttc tctggcagct ctccacgaaa
    33121 atcaactact tgcgtttccg aatggataga cattgtttca ttcccaccgt aaaaaatcaa
    33181 ttcattgctt tcatcattat ttttcacgct atctggtttc tggaaaaaag catttctaaa
    33241 aagtgtatag ggttgctgtg acaggatata actatacttc ttcagtttga tcgaatgttt
    33301 cgtagcatat tgcgtgtcga tcgtcgatgg ttctgttgtc atttctgtcc attttgtgtc
    33361 ttttgctgtt aattctttat tgatatctgt ccaattcaac gaaatatttg cttcatatac
    33421 gagatggttc gaataattca aaaatcggat ttttttattt ccttgatcga attgtattct
    33481 agtaaaatac atattttcgc tttcttttgc caagttcgac acatctaaat ctaaatcaaa
    33541 tgcttccttg tattctctta gcaaaaaagg cgcattatag gccaactcaa aaccagagtt
    33601 cagatttttc acctgttcaa actgtgattt gtctcctgaa actacttcag aaagctttcc
    33661 aaatcttgct ttatcgatga tcaattgcaa tcggtacacg agattttcac tgttggtttc
    33721 ttggatcatt cccgaatggt gccatgtaac atgaagcgga agaaagactt cggaagtttt
    33781 tcgattgctt tgagactcga tcatttgagt gtttgtctca tctacgttga ttgaactttt
    33841 tcctgctggg ctcagccaga ttgcataaga gaagtatatg cttaacgcaa ccatcaaaat
    33901 gagtcctata cgaacgactt tttcagagat tctcattccc accattcctc ctcataaggt
    33961 tcatatggca gcgagatata aaaagtcgac ccttttcctt ccttgctctc tgcccaaata
    34021 gcgcctcgat gcgccttaat cacttctcta gaaatagcta atccaagtcc tgtcccacct
    34081 tgtgctcttg cacgtgcttt atctactcga tagaaccgct cgaacactcg gttcagatct
    34141 tttttaggaa tccccaatcc ttgatcggtg atacttaaaa tcacgttatt atgcgtttct
    34201 aataagcgac aagtaatcgt tcccccatct ggtgaatact tgatcgcgtt attcatgata
    34261 ttatcgacta cttggatgat tttatctgta tctacttcga cccaaagatc tctttgcgta
    34321 aattcacgac ggatcttata atttttttcc tgatttccca ccatcatatc aaaccggtca
    34381 agaacaaaat tcactaactc gttgaaattg acatattcta attgaagctg ggtattacct
    34441 gtatccatgc gagatagatt taagaggtca ttgatcattc gaatcattcg gtctgtttca
    34501 tctagcgtta ccttcaaaaa atttggtgct acttcctcgt ctttccacgc accttcgctc
    34561 aatgcttcga tataactacg catacttgtc aacggtgtac gaagttcatg ggaaacatta
    34621 gagacgaatt cacgacgttc ccgttctgtt ttttcttgtt ctgtcacatc atgcaagaca
    34681 gctactaatc cgctgataaa acctgattcg cgacggatca tagaaaagtc tactcgcaag
    34741 atcatctgat cactttccaa tgtactggtt gaacggtcaa gtaccatttc gtttggatct
    34801 tctaataatt tccgcaacgt atattcttct tcgatatcta aaagtgtcaa aatcgattgt
    34861 ccgattgctt cctcgttttt gatatctagc aaggaaacag ccatatcatt gatcgtaatg
    34921 actttacccc tgcgatctgt cgcaatgacg ccgtctgtca tatgggatag cacgctatcc
    34981 aaacgattgc gttcagcttc cattgtatct tgagcttctt caatacgttc agctaactga
    35041 ttgaaggtat cagccagctg tccaagttca tcttgtccat gcactttgac tttccgactg
    35101 tagtctcctc tagcgattcg aatggcttgt tctctcattt caccgatcgg ctgtgtaatc
    35161 gaacgagcaa ctaaaacaga tacgatcatt gaaataaatg ccgcaattag tgaggcagta
    35221 aagaaaatga ctgctgtgtt attgatttcc gaatattttt gttcgatatt acttttgacg
    35281 taaagagccc cgataacggc atcgccggtc ggggacagga tcggttgaac attgatattc
    35341 acccgtttat cgtcgttatc taaagcaaca taacgtttag tcgtgaaatc attgatatca
    35401 acataatcat ttttctttcc cacagcgctt tgttcattga catcggttgt tcctcggatg
    35461 atcccttttt cgtcaacgac ccgtatttcg atgatatcgc tagtcgcact gttttcaata
    35521 aatcttttga tattcgcatt tacctcttca tcatctgcat tatcttttcg attcaattcc
    35581 gggctgattg ttgttgctaa tgattccacc gtttgattca tatctttaat aaatgtattg
    35641 atcgtggaac gttctaaacc acgtataaag taggcaccaa taatttcgat cgagatcaat
    35701 aaaatcagaa taaaggtaat agcgatctta aaattgactg atcgaaaaaa gcggactttt
    35761 cttttcatcc aattactcct gttctgggtt acgcaaatag taaccgacac cacgacgtgt
    35821 cactaaataa tttggatggc ttggattatc ttcgatcttt tcacgcaaac gacgtacagt
    35881 tacatccact gttcgtacat ctccaaaata atcataaccc caaaccgttt gtaataaatg
    35941 ttctcttgtc atcacttgtc caaggtgctt cgccaagtaa aagagtagtt cgaattcacg
    36001 atgtgttaac tcgattgttt ctcctcgttt cgtcaccatg taagcttctg gatggatcgt
    36061 caaatcacca atcgtcaatt ccgcaggtgc tgcctcttct ggttccttcg cagccgtggc
    36121 accgcgacga agatttgctt tgacacgtgc aactaattct cggttggaaa atggcttggt
    36181 cacgtaatca tcggcgccta actctaagcc taggacttta tcaatttcag aatctttggc
    36241 tgttaccata atgatcggca tatcataagt tttgcgtacc tcacgagcaa cttctaagcc
    36301 gtccatttta ggtagcatca aatcaagtaa aatcaaatca ggttctacct cagcaacttt
    36361 ttccaaggct tcttccccat catatgcagt ataaacgtca tagccttctt tggctaaatt
    36421 gaatttgacg atatccgaaa tcggcttctc atcatcgaca actaaaactt ttttcataaa
    36481 gagccacctc ttatatatta ttgtcatacc ttctcattat accgtatttc tgcttttgtt
    36541 tcatctttgc ctataaaaca aaagagaaaa ttaaaatgaa agtgcaaaat actttattat
    36601 tttttgttct aacttcaata aaaaaggaaa acactgattt tcacctcagt gtttcctttt
    36661 tttgatttat ccttctaaaa cttgtacacc gacacccaat aatcccggac cagtgtggac
    36721 tactaatgcc ggagaaatct ggccaaagta gatttggttt gcttgtggga actctctttt
    36781 aagtctcgct tccatttcaa ttgcttcttc tttcgcatcc ccatgggcaa cagctaagtt
    36841 gaagaccttt gcattaccga ttttttcttt gacgtaagca accgttttat ccaaactttt
    36901 tttacgtccc ctagcttttc ctactgtgta ataaatccct tcttcattac atgagatgat
    36961 tgggttaagc tttaaagcgg ttcctacaat agaagcaact aaaccgatcc gtccaccttt
    37021 ttgaagatat tccaatgtcg caacattaaa gaaaactttt gttctttcga ctaactcatt
    37081 cagattactg atcagttgat cccattctac tccttcttcc agccattttg ctgcttggat
    37141 cgcagaaaaa cctgcaccaa taccaatatt tttcgtatct agtacatatg cttccaattc
    37201 ttcttgctgt tgtgcaatta aacgaaccac attatatgtc ccgctcaatc cactggaaat
    37261 tgttaccgca agtacctttt cataaccgtc ttttttgatt tgctcaaaga tttctgtgat
    37321 cgactcacca tccggcaaag atgtaccagg gatttcctct ggcaaacgtt gatagatctc
    37381 ttcaggggta atgtcaattt tatctgtata tacctgatct ttataaataa ttttcaatgg
    37441 gatcatatac atctcgtatt tttttatcaa ttcttctgga acatccgtac ctgaatctac
    37501 taaaattgcg attttattgt ttttcatttg ttttctcctc ttttaaatat tcggtttcta
    37561 gaacaatcgt tttttccgca agaagtttca ttgcaaacga taaagttgcc gctcttacag
    37621 caatatattc tacaggggtt gcctggtcga atttttgcgg atgagattta ccttgtgcta
    37681 attggctgac catccacaca gctgcttctt gttcatcgca aaatagatta taggcttcac
    37741 gaattcctac tgtttttcct tggaacaata tcccttcttt gatttcagga atagtcaata
    37801 cttgtttcaa cgtagtgatt gcaagtaaaa atgctacgtg ttctttgtta tatctttttt
    37861 taactggtgc tggtatcaaa cctagtttaa cgtaattgtt taccatagaa gaggtaagca
    37921 atggatgctt ttctccttgt atcacaggtg aaagataacg atcaactaac gttctgactt
    37981 ggtccatata aagttctaag tcagggagtt cttcccagtg aggcagacgg aatgtttcta
    38041 gttccttccc ccactccatt aattgctgac gcattttttc catcataacc gctccttttt
    38101 tcatttcata aacatgatat cagacttatg ataaacattc aatctagttg ctttaactag
    38161 gtgatttgat tagcgggatt ccatgaaaac aaaaaacaag aaggctaagc cttcttgtct
    38221 tttatcgtta agaacaggag acccgccggg gagacggcgg gaaaggagta aaaaatgaaa
    38281 aaagtgttag ggtttgttct cttgcaagaa ctttgtttgt tggtatgctt atataatagc
    38341 gagcaaatgt gaataatcta tgaatgattt cctataagga agtaaagatt tttctgattt
    38401 tctttaaatg tagtcttgca ttttaaaatt cttagtgcta ctattattaa gtactttttg
    38461 tacgggccgt tagctcagtt ggtagagcag ctgactctta atcagcgggt cgcgggttcg
    38521 agcccctcac ggcccattgg gtgccaaacc cacggaacag gtaaatgttg gtgcgaaagc
    38581 atttcgaaaa catttacgct gttctttttt tatttttcaa aataactagg gctttttatt
    38641 tttaactcaa agtccactgt agccctttgg actttgagtt aaaaaagaag cataattatg
    38701 aacagtaaag tttctaatat aacaataatt cgagaagctt tgcaaaatca tgctgccaat
    38761 gaactctcta aacatatagg attaaatcta agtactataa aaggttaaaa agtgaagaat
    38821 atttaataga aaaactaaat ttacttgctg ctttcccctt ttccattaat acaacaatca
    38881 gagagtgtct ttttttcgaa aaaattagaa taaataaaag acacgtagtt tttttatcta
    38941 catgtcttcc atcgactaca agttatcatt tctcaaaacg tatttagctt ttaattgatt
    39001 aatcgtttca cgttgtctac acattgccta attaagattt tcttattaga agcatcaatc
    39061 ccgcaataat acctaaaact ccaggaatta cccacaataa tgttaaaaga aaaaatggaa
    39121 taatagataa aatgagtaaa ctaataccat agaccactgt tttttgattt tttcttacta
    39181 aaaaacctat tatgaaaatc attatcgaaa aaactagggt aattcctcca aataaaacaa
    39241 cattcccgct tagattgact aacatatcta tatccttact ggttagttct gtagtattta
    39301 aagtagttga atcaatagaa gatagaaaca taccacccat caatgctaaa agactacata
    39361 aaatccctgt tattccacta attaaaatca aaataaactc tgtttttctc ttattcattt
    39421 aattctctcc agattaagtg ttttagaatt agaaattttt tgaccatagt taatagcttc
    39481 taatgaacga ataacaacca ctgacgtgtt agaaaaataa tccccaatat gttgtttctt
    39541 gggcgtgaaa gcagccacca aatacccaat ttgaaataca aaatttttta atataaacct
    39601 acatatacac tcacgaataa agatagtttg ccaacttagc tctttttcct caaagctcac
    39661 tactcttatc ccaaaaatca ttttcccaat tgtttgtcct gaattcagct tagtcaaaag
    39721 tacaaaatag gttaaataaa tagctaatga cgctatacca taaaaagtaa taatggattt
    39781 gtcagattcc agtcccatga ttttgtaaaa gagacctata gtagcatggg tgatgatgct
    39841 aatacaaatt aaatcaataa gaaaagcaaa aaatcgtaac caaaatcccg caaatacata
    39901 attaggcaaa gtttcaatgc cactcttctt attcatttta tagccctcca tagtaataca
    39961 ttggttttgg agtatcagaa aatccaagtt tttcaaaaac tgaaattaca cgatctgatg
    40021 tagttgcctt tagcccttgc cattcagcaa tcttattacc taaccaagaa gaagcaaagc
    40081 cagttgtgtc atttttatat tctataaccg tcgcatcagt taatttcttc tcttttttta
    40141 gggaatctaa tgcatcttca gagtaaccaa tttgatccac caacccattt tctactgctt
    40201 ggttaccatc ataaattctt ccatccgcaa gttctttcac tgcattagta tccatatttc
    40261 ttccttttgc tacaacattg acaaaacgat cataagcact catcacaaat tcctgtataa
    40321 ctttattctc ttcctctgaa ggtttgtgtt gaggtgacat catatctttt aatgcaccac
    40381 ttttatacgt cgcatcagtt acgccaagtt tttctaataa gccagaataa tttaaaccag
    40441 aaataatgac accaattgat ccagttgttg tttcttccgt agcaaatatt ttatctgaac
    40501 cagcagaaat gtagtaccct ccacttgccg ctgtattttt aaatgcagta tagataggaa
    40561 tatctagctt tttaattttt gccatttctt tagcaatctc agcactttca tatattcctc
    40621 ccccaggtga atttacttct aataacactc ccttaaccgc tttgtcttct tgaattttct
    40681 ttagctgtgt taaaaaattc tgatggttat attgttccct actaaaaaga ttgctttcac
    40741 ctgtatctgc aataactcca ttgacggata gtttgacaat ctttttattt gatgcgcctt
    40801 cttctaacgt ttcttctgtg atttcgttag atccgtaaaa aatcttattt attcctgtta
    40861 gggttgtatt tgatgcttcc tctttatctt ttcctggaat tagcgcgacg ataatggata
    40921 gtaacaataa tccgcacgct actcccacag caatccatct tcttttattc atataaactc
    40981 ctcctatatt cttagtaaaa ataattattt tttatttaga aacacatcga caatttgttg
    41041 ccaatatgtt ttttctccta caattaaaga caactctcca gttaatccat aacgaaacgg
    41101 aaacgattga gttgttttta acgttccttt cactgcataa aaaacacctt tctcagtggc
    41161 agtagaattt tctgaaattt catctaaagt tccgtcgatc gtaatcggag ccccaccttt
    41221 ttgatccaat ttaaaatgaa ccttcatccc ttctttaata taggtacttt cattcgcagg
    41281 taaaagtgct gtaaatttca tttttgattt ttcgtggatc ggataaattt ctgcaagcac
    41341 tgttcctttg gctagttctg cttgtccctt tgtttcttca tttaaatgaa ccgttccaga
    41401 tacaggagct tttagtattc cttttgaaag ttgatcgtcc aaacttttga taactgactg
    41461 gttcttctct tgtttttcat ttagctctgt aactgtttgt tttacagttg ttattgcttg
    41521 ttctagctct tgctttcctt tttccgtttg actaattctg tcattctcag aagtagttgg
    41581 tttaactaat ttagcttttt ctgactgata ctgttctact tcttttttta gttgctcaat
    41641 ttgttcattt atgcttgctg aaattgtcag tttcacttga tttttttgat catcagaaac
    41701 attatttagt tgttcttgcc aattctcata gtttgccatc atttcttttg aaaaatcttt
    41761 taatgattga ttattactcc aagctacctg tacttgttgc cattcttgta atttactatc
    41821 tgaactctca atttgttttt ctagttgtct ttttgtcttt tcgtatactt ctaattgctt
    41881 ttgatcattt aattcacttt gcttcaaagc gtagcggagt gattcttttt ctgacaacat
    41941 gctctttaat tgattactgt agccaaaact gtcatccgtt gaaaacaaat tttttccttt
    42001 acttatactt tcaataaata gctgagccat ctttatttgt tgagtaatac gttggttttc
    42061 ttgttctatc tgagcctttt cattctctat taaagaacaa tccaatctaa ccaacacatc
    42121 gtccttatga acaaattgat tttcttttag gaagttttcc attattttag cttctaatgg
    42181 cacttgtaac ttctctattt tttcggggtt caattgagcc gttgtccgaa taacaacctc
    42241 ttttttagca aaggttaaaa acaatccaat aatacataaa aaaagaatta cagggtataa
    42301 cacccaacgg tagaagctat gatgttgttt agtatagata ctagaatgtt cgaaccaatt
    42361 attattctgc ataaattgtt tccctctcct agatttccca taatttttga tagataccat
    42421 cggcaaaacg aagttcatcg tgcgttcctt tttctaccaa atgcccttca tggagaacta
    42481 aaatttgatc acatgctttt gctattggta aatgatgcgc aataaaaata atcgtttttt
    42541 cttttaaatt gaggagataa ttcataattg catgttctaa caaggtatct aatccactag
    42601 ttgcttcatc taaaattaat atgtccggat tttttaataa agcccgtgct aaggctaatc
    42661 gttgtcgttg ccctcctgaa agattatttc caccttcttc caacaatgtc tcgtaacgta
    42721 atggctgttg attaataaat tcatttagtt ggactatctc acaaacttct attatttgtt
    42781 cgaaacttgg tgggctatct aaaccgaata taagattctc aatgattgtt ccactaaaga
    42841 aaaatgactc ttgaggcaaa tatgtcactc gatcgcgtaa tatctgaaaa ggaatgtcta
    42901 agtgattaat gtttccataa tggatcgatc cttctgatgc ttcataaaaa cgaacaaata
    42961 actttgcaag agttgatttt ccagaaccac taactccaac tatagccgtt ttactgtgcg
    43021 aaggaattgt acatgaaatt tcgttcaacg ttggggcctt catgctatac gagaatgaaa
    43081 cgttttttac agtgatatct tgttgaaata attgttcaga aagcatatat tcaaaagact
    43141 ggttcgattt ttctgaatca atcgcgaaaa tttcatttaa acgattattt gcaacttgag
    43201 ccgtttgcat tttcacttgt aaattaataa tgttttgtaa aggatctgtc aaaaatacta
    43261 gtaaagcatt gtatgtgata agttgaccta gactaattct attatccata acaaaataag
    43321 aacctagcca taaaattaat gcactgctaa ttaattgaat caaatgttta attccttgtt
    43381 gaatattatc aagcgtaacc gtacgaaatg attttttcat taatttaaca aattcatggt
    43441 ctatacggtt atatactttc tcttcaccgc tatatgcttt aattgtttct attcctttaa
    43501 gactttctat gatactagaa tttacagctg caccggctgc catttcttcc ctgttcgatt
    43561 tttcatagct tttcacgaaa gcgaatattg caactacata aaaaggcaaa gagcatagag
    43621 tgattaaaaa caacgtacta ttctgaatag ctaacgtaac cccaacaagc aacaccatac
    43681 caatatctaa aaatattgat aacgtagcac ttgctaaagc atcaataatc ttactggcat
    43741 caagaaaacg agaaataatt tcccctgatt ttctcgtagc aaaaaaattc attggtaaag
    43801 ttaacacatg tttgaaatac tgaagcataa ttgccatact cattcgctgt cctaaaataa
    43861 taagaagata actacgacta tattcaaaca atacccgaaa taaatagaca gcgatcaagc
    43921 caatggaaat tatatttaaa gttgaacgtg cttgattagg gatgaaaaaa tcaagaatac
    43981 cttgaaaata atatgagctt cctatgccaa aaaacgtaat gaaaatcgat gctaaaacga
    44041 tgtggaatac taatcctttt tgtttccaaa ttataggcaa aaaagaactt aatccagcta
    44101 ctttttcttt aataggaata taagtttctc taggcatagg taataaaaga atacctgtcc
    44161 attctttttg aaacgcttca atcgtttttc tattttttcc attagcggga tctgcaatcc
    44221 atagtacgtt tcccttcgtt ttataaatta caacgtagtg catatagctg ttatcaacca
    44281 atatatgtgc aattaatggt aagataagat ctctttcttt ccagatctca ctatctgctt
    44341 gaatagccat acaatcaaaa gacaattttt caaatgtctt tttcaatcca aatgctgaag
    44401 taccctctaa atcagtccca gataggtccc gtaatttatg aatcgggata tcagttttaa
    44461 aatattttaa aatcatcgaa aggcaagcaa caccacaatc tttttcatct tgttgctgca
    44521 caaaaggaaa tgattttatc acttatctaa cacaactcct tctaaaaaat ttttatagaa
    44581 aaattctcaa tatagcttta atacagtgaa aatattgtct cataattcca caaaa
//
LOCUS       DACDUU010000015        41316 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.015, whole genome shotgun sequence.
ACCESSION   DACDUU010000015 DACDUU010000000
VERSION     DACDUU010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.015"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(531..2027)
                     /gene="lysS"
                     /locus_tag="IWL30_000859"
     CDS             complement(531..2027)
                     /gene="lysS"
                     /locus_tag="IWL30_000859"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP9705645.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(2138..3142)
                     /gene="dusB"
                     /locus_tag="IWL30_000860"
     CDS             complement(2138..3142)
                     /gene="dusB"
                     /locus_tag="IWL30_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAP9705646.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(3143..4030)
                     /gene="hslO"
                     /locus_tag="IWL30_000861"
     CDS             complement(3143..4030)
                     /gene="hslO"
                     /locus_tag="IWL30_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAP9705647.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4247..6358)
                     /gene="hflB"
                     /locus_tag="IWL30_000862"
     CDS             complement(4247..6358)
                     /gene="hflB"
                     /locus_tag="IWL30_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAP9705648.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6448..6993)
                     /gene="hpt"
                     /locus_tag="IWL30_000863"
     CDS             complement(6448..6993)
                     /gene="hpt"
                     /locus_tag="IWL30_000863"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAP9705649.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6993..8438)
                     /gene="tilS"
                     /locus_tag="IWL30_000864"
     CDS             complement(6993..8438)
                     /gene="tilS"
                     /locus_tag="IWL30_000864"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAP9705650.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8558..9028)
                     /locus_tag="IWL30_000865"
     CDS             complement(8558..9028)
                     /locus_tag="IWL30_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAP9705651.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(9074..9499)
                     /locus_tag="IWL30_000866"
     CDS             complement(9074..9499)
                     /locus_tag="IWL30_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAP9705652.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9566..9835)
                     /locus_tag="IWL30_000867"
     CDS             complement(9566..9835)
                     /locus_tag="IWL30_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAP9705653.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9846..11441)
                     /locus_tag="IWL30_000868"
     CDS             complement(9846..11441)
                     /locus_tag="IWL30_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9705654.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11456..14977)
                     /gene="mfd"
                     /locus_tag="IWL30_000869"
     CDS             complement(11456..14977)
                     /gene="mfd"
                     /locus_tag="IWL30_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAP9705655.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14994..15554)
                     /locus_tag="IWL30_000870"
     CDS             complement(14994..15554)
                     /locus_tag="IWL30_000870"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP9705656.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15907..16881
                     /locus_tag="IWL30_000871"
     CDS             15907..16881
                     /locus_tag="IWL30_000871"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP9705657.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            17051..18451
                     /locus_tag="IWL30_000872"
     CDS             17051..18451
                     /locus_tag="IWL30_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAP9705658.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18529..19251)
                     /locus_tag="IWL30_000873"
     CDS             complement(18529..19251)
                     /locus_tag="IWL30_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9705659.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19330..19836)
                     /locus_tag="IWL30_000874"
     CDS             complement(19330..19836)
                     /locus_tag="IWL30_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP9705660.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19959..21758
                     /locus_tag="IWL30_000875"
     CDS             19959..21758
                     /locus_tag="IWL30_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAP9705661.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21826..23400)
                     /locus_tag="IWL30_000876"
     CDS             complement(21826..23400)
                     /locus_tag="IWL30_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705662.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23655..24164)
                     /gene="mreD"
                     /locus_tag="IWL30_000877"
     CDS             complement(23655..24164)
                     /gene="mreD"
                     /locus_tag="IWL30_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAP9705663.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(24168..25022)
                     /gene="mreC"
                     /locus_tag="IWL30_000878"
     CDS             complement(24168..25022)
                     /gene="mreC"
                     /locus_tag="IWL30_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAP9705664.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(25177..25815)
                     /locus_tag="IWL30_000879"
     CDS             complement(25177..25815)
                     /locus_tag="IWL30_000879"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAP9705665.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25816..26466)
                     /locus_tag="IWL30_000880"
     CDS             complement(25816..26466)
                     /locus_tag="IWL30_000880"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP9705666.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(26479..27378)
                     /gene="rsgA"
                     /locus_tag="IWL30_000881"
     CDS             complement(26479..27378)
                     /gene="rsgA"
                     /locus_tag="IWL30_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAP9705667.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27543..29612)
                     /gene="pknB"
                     /locus_tag="IWL30_000882"
     CDS             complement(27543..29612)
                     /gene="pknB"
                     /locus_tag="IWL30_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAP9705668.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29609..30349)
                     /locus_tag="IWL30_000883"
     CDS             complement(29609..30349)
                     /locus_tag="IWL30_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAP9705669.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(30362..31720)
                     /gene="rsmB"
                     /locus_tag="IWL30_000884"
     CDS             complement(30362..31720)
                     /gene="rsmB"
                     /locus_tag="IWL30_000884"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAP9705670.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31720..32658)
                     /locus_tag="IWL30_000885"
     CDS             complement(31720..32658)
                     /locus_tag="IWL30_000885"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAP9705671.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32651..33142)
                     /gene="def"
                     /locus_tag="IWL30_000886"
     CDS             complement(32651..33142)
                     /gene="def"
                     /locus_tag="IWL30_000886"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP9705672.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(33157..35565)
                     /gene="priA"
                     /locus_tag="IWL30_000887"
     CDS             complement(33157..35565)
                     /gene="priA"
                     /locus_tag="IWL30_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAP9705673.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35818..36123)
                     /locus_tag="IWL30_000888"
     CDS             complement(35818..36123)
                     /locus_tag="IWL30_000888"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP9705674.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(36128..36742)
                     /gene="gmk"
                     /locus_tag="IWL30_000889"
     CDS             complement(36128..36742)
                     /gene="gmk"
                     /locus_tag="IWL30_000889"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAP9705675.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRRAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(37229..37513)
                     /locus_tag="IWL30_000890"
     CDS             complement(37229..37513)
                     /locus_tag="IWL30_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705676.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37531..38421)
                     /locus_tag="IWL30_000891"
     CDS             complement(37531..38421)
                     /locus_tag="IWL30_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAP9705677.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(38441..38659)
                     /locus_tag="IWL30_000892"
     CDS             complement(38441..38659)
                     /locus_tag="IWL30_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705678.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38629..40329)
                     /locus_tag="IWL30_000893"
     CDS             complement(38629..40329)
                     /locus_tag="IWL30_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAP9705679.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40596..>41316
                     /locus_tag="IWL30_000894"
     CDS             40596..>41316
                     /locus_tag="IWL30_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705680.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTD"
BASE COUNT        11227 a         8912 c         7481 g        13696 t
ORIGIN      
        1 agagtgtttc ccgattcatt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagcgattgt caactctttt ttaaaagttt ttttcgattt cgtttttcga aatctttcgg
      121 caactcattt atcataactt atcgtttgtt atttgtcaag aacttttttg aagttttttt
      181 cgatagaatc aagcttcgca agtggttctt ttttaacgac tcagttatct tatcatgtcg
      241 ttagtaacat gtcaataact tttttgttat gtttttgaat aaatattttc ttctgtcttt
      301 tggacaagta ataatatacc aactaaattt agcttggtca acgaattttt tgtatttttt
      361 caagtttttt tcaataaacg attattcata acttttcttt ctttatcgtt cgttttcgtc
      421 ttctttaatg tctagaatag tcggcattag cttttgaact ttcgttatta tttattcctt
      481 tctacacagc aaaagctgcc taaggaattc cttagacagc ttttattcga ttatctcatt
      541 gttgggaata gcaaaacatc ccgaattgat tgtgcatcag ttagcaacat tactaatctg
      601 tcgatcccga tgcctagacc accagttggc ggcataccgt attctaaggc ttccaagaag
      661 tcttcgtcta caccgtgagc ttcgtcattt cctagttcac gttctttttc ttgcgcttcg
      721 aatctttctc tttgatcaat tggatcgttc agttctgtaa aggcattagc aaattcgcgc
      781 cccactatga atagttcgaa tcgatctgtg aatcgaggat cttctggatt tttcttcgct
      841 aaaggcgaaa cttcgactgg atgtccgtat acaaacgtcg gttgtgtcaa tgtttcttca
      901 acaaatgttt caaagaattc attgataaca tgtccgacac ccatagcttc ggttacttct
      961 acattgtgtt tttcggctaa agctaatgct tcttccaatg tcatttcttg ccagaagtct
     1021 acaccagttt gttctttgat tgcatctacc atatggatgc gtttaaagtc actttctaag
     1081 tcgacagctt gtccatcata tgtgattttt gctgttccta gtactttttc agctgcgtta
     1141 cggataatac cttctgtcag attcattaca tcattgaaat cagtatatgc tgtgtatgct
     1201 tctaacatcg taaattctgg attatgtgta gtatcaattc cttcattacg gaaaacgcgg
     1261 ccgatttcat aaactttttc cattccgcca acaattagtc gttttaaatg aagttctagc
     1321 gcaatacgca aatataaatc aatgtccagt gcattatgat gcgtgataaa tgggcgtgcg
     1381 gcagctccac ctgcctcatt gtgcaatact ggtgtttcta cttcaatata gccattgcca
     1441 tctaaatagc gacggatttc gctgataatt tggcttcgtt tcataaatcg gtcaaagctt
     1501 tcgcgattac tgattaaatc caagtagcgt tgtctgtagc gttgttcaat attcgttaag
     1561 ccatggtatt tatctggcag tggacgaagt gcttttgaca aaatcgtgat ttctgaagct
     1621 ttgatagaaa cttcgcctgt gtctgttttc atgatttgtc cagttacacc aaagaaatct
     1681 ccaaggtctg catgtttgaa tacttcatag gcctcatcac ctacttggtc ttttcttaca
     1741 taaatttgaa tttggccttc acgatcttgt agatgcgcaa atccagcttt cccttttcct
     1801 cggcgagtca tcatacgacc agctacacta gcggtcaatc ccatttctgc tagttcttct
     1861 tttgttcttg gatcaaatag ttcatgcaat tcttctgagt tgtgcgtgcg ttcaaatcgt
     1921 tttccaaatg gatcgattcc atcttcacgc aattgttcca ttttttgacg acgtacaagc
     1981 atttgatcat ttaaatcttc tacttgatgt tgttgttcct gtgtcacaaa agttcctcca
     2041 ttctcatgtt aatctctttc ctataatgcc aatgaaacac aaaaaaagca agccgaactt
     2101 ggaatttcgg cttgaaaatt cacgaaaaac ttagaaatca caacgtttgt ttcaatagta
     2161 ctttttccgc tttttctaca aattgatcca gcaggtcatt gatttcttgc tgtttttctg
     2221 tctgattgat tgctactttc actttcgctg ctcgaggaat cccttttaaa taataagccg
     2281 catgtgttct gaattctcta caagctactt tttctccttt gagatctgct aagcgttgta
     2341 aatgaagttt agctgtagca attttttcgg ctggtgttgg ttcaggaatc agttcccctg
     2401 tttcgagata atgctgtgtg cggtgaatca tccatggatt gcccagcgct gcacgaccaa
     2461 tcatcacacc gtctgcaccg acatagtcaa gcatccattt tgcatcttct ggcgttttga
     2521 catccccgtt ccccataaat ggaatcgtca aatgtttttt cacttctttc aatacttccc
     2581 agtccgcttt tccttcatac atctgaaccc gtgtacgtcc atgcatagcg atggcacttg
     2641 ctccagctga ttgtgctgct aatgcatttt ctacagcaaa gacgtgctct tcatcccaac
     2701 caatacgcat cttgattgtt actgggatat ttactgctga agaaacagct tggaccattt
     2761 cgtatacttt ctcaggatct aaaagccatt ttgctcctgc ttctgctttg attactttat
     2821 ttactggaca gcccatattg atatctatta tatcggcagc tgtgttttct tcgacaaatt
     2881 gagcagcttc aactagacta tctttatttc cgccaaatat ctgtaaactt aatggatgtt
     2941 ctgtttcttc aatgtggagc atctctaaag tttttttgtt tctgaaatga ataccttggt
     3001 cactgatcat ttcacatacg actaagcccg caccaaattc tttgacagtg actcgaaatg
     3061 ctgaatttgt tattccagcc atcggtgcaa caacgacacg attagggatt tccacattgc
     3121 caattttcca tgtgtgttcc acttaaatcc ctcctaaggc ttctttcttc aatccatcta
     3181 attcttcttc gctatacttg tatttgttat tacagaaaga acaaaccgct tctgcaccgt
     3241 gatcttcgtc tatcattgct tggatttctt ctgcacctaa cgcgataatt gcttctgcaa
     3301 atttttcttt tgaacagtca catttgaatt gaacaggcat tttttccaaa atgttcaatt
     3361 cttctgtacc aagcaatcct ttcaatattt cttctggtgt ctgtccttca tctaagaggg
     3421 tagagattct tggtgtcgtt tttaattgtt cttcgatctt gctgattgtt tcttcatcgg
     3481 cacctggcat gatttggatc atgaatcctc ccgccgtctt gatgctgtcg tcagtattga
     3541 ccaatacact cagtccaaca gctgaaggga tttgttctga gatagcaaga tagtaagtaa
     3601 aatcttcagc gatttctcca gaaacaatcg gcgtttgccc agaaaaaggt tcttttaatc
     3661 ctaagtcttt tgtgacagta aacattcctt gtgttccaac tgctccccgc acatcgattt
     3721 taccttcact atttagagga agactgagat gcggattttt gatgtaacct ttcacatctc
     3781 cctttccatt tgcatctact acaattcctg ttgctggacc atcgccttgg attcgaacag
     3841 ttagtttgtc ttctcctttg agcgtcgcac caagtagcag actaccgacc aatgtccgtc
     3901 ccaaagcagc tgaggaggtg tgccaagtat catgacgtct ttgtgcctcc gctacggtat
     3961 ttgttgcttg aacggcgtat gctcggacga atccgtcgtg agcaagtgcc ttaaccaaat
     4021 aatctttcat ttgttatgta ctcctttttc cgtttttaaa ctcatagtga atcatactag
     4081 caactactgc ttttttcaac tttggaatgc ttcttttaaa aataattcag aatcatctag
     4141 aaaacaggac atcaccctat ttacaactta aataaagaaa tctcccgaaa accggttttt
     4201 gttccggttt tcgggagtca tatcagatca attgacctat atttttttat ttgtattgat
     4261 cgtctgaatg attatcttct gattgttgag aatctgaatc atgttctgct ttattttcct
     4321 cattcagttc atgttttgct tcttcaaaat cacgtttttc ttccgcttgt ttttcagcgt
     4381 ctttttcttc caaagcacgt tttgcttctt caaaagtttt cgcttctttt tcgcttggat
     4441 aatcatttga ctcagtacct tcaggcatca caccgtgttc aaacaatgac ttgattgctt
     4501 tggcatccaa tgtttcgaat tccaaaagtt tttcagcaat cagtttatgc tgtgcacgat
     4561 gagcttcgat gatttctcga gctttatcat gagcttccat caagattttt cgtacttctt
     4621 gatcaatctc gaaagcgact tgttccgagt aagcttttgt ttgaccatag tcgcggccaa
     4681 caaacacttg atggtttcct tcatattgaa caggacctaa acgatcactc attccatatt
     4741 ctgttaccat gcttcgtgct agagctgtcg cttgctcaaa gtcattggaa gcaccagttg
     4801 attggacatt gaagatgatt tcttcagctg tacgtccacc aagtaagcct acgatttgtt
     4861 caaacatatc ttctttcgtc atcaagaact gatcttcttt tggtaaagcg atcatatatc
     4921 cgccggcgcg tccgcgaggg atgatcgtta ctttgtggac gacccgagcg cgactcaata
     4981 ctaatccgac aatcgtatga ccagcttcgt gataagcaac catttcgcgt tcttttttgc
     5041 tgattacgcg atctttttta gctggacctg caatcacgcg atcttccgct tcatcgatat
     5101 cagaagcatc gattttcttt ttatttcttc tagcagctac taacgctgct tcattcaaga
     5161 cattttctaa atctgctccg gcaaaacctg gtgtctgttg agccacaact ttcaagtcaa
     5221 cgtcatctgc catcggttta ttccgtgcat ggactcgcaa gattgcttca cgacctttta
     5281 catctggacg tccaactaaa atctggcggt caaaacggcc tggacgtagt aacgctgggt
     5341 ctagtacatc agaacggttg gttgcagcaa tgacgattac tccttcattt ccgtcgaagc
     5401 catccatttc tacaagcaat tggttcaatg tttgttcgcg ttcatcatgt ccgccgccca
     5461 taccggcacc acgttgacga cctaccgcat caatttcatc aatgaaaatg atagctggtg
     5521 cgtttttctt cgctgtttca aacaaatcac gaacacgact tgctccaaca ccgacaaaca
     5581 tttctacgaa atcagaaccc gagatcgagt aaaacggtac tccagcttct ccggcaacag
     5641 cttttgctaa taaggtttta cctgttcctg gaggtccttc taaaagtaca cctgccggga
     5701 tacgggcacc taattcaaca aatcggcgag gatctttcag aaattcaaca acttcaacta
     5761 gttcttgttt ttcttcttcc gcacccgcta catcagaaaa acgtacgcga ttagcttttt
     5821 tgtctgcttc tttggctttt gatttaccaa agttcatgac acggccgcca ccgccaccgc
     5881 cgccgccttg ttgtcccatc atcatgtaaa agaaaaagat gatgattacg attggcaaga
     5941 agctaagcaa tagggaaacc catataccgc tgcttgattc ttcttttact gttgtagcaa
     6001 ctttgttttc ttttgccagt tctgttactt ggctcaatgt tgtatcattt ggcaaaatga
     6061 ttgttgtgaa atgggtagta gtcgtatctg ttgaacctaa gatagctaac ccaccgctgt
     6121 tagcgacggt ctgctgttct ttgtattcac cagtgatctt atatacacca tttgcaggct
     6181 gcacttcaaa ttctttgatc ttgcctgctt gtagttgttc tgtgaatttt gagtactcga
     6241 tatctgacga tcgctggccg ttattaccaa aaatgaaata aacgaccatt accatcgcta
     6301 agattacgag gacataatac aaggcatttt tcatcatgcc gttgttcttt ttgttcatag
     6361 ttgccctccc taagcgtcaa tttaatttaa cagtttttta tcaatcctga ccattctatt
     6421 gtaccataat ttaattgaga tacagcatta atttgattga taaacactag gtttcaatac
     6481 accgatgtac ggaaggttac gatatgcttc tgcataatcc aagccataac cgaccacaaa
     6541 ttcatttggt acgttgaagc caacataatc tgcttcgata tttactactc ttccttcagg
     6601 tttgtcaagt agtgtaacaa ttttgactga ttttgctttg cggtatttga aaagatccac
     6661 taaataagca agtgttcttc cgctgtcaat gatatcttca acgatcaaca gatcgcgacc
     6721 ttctacattc gtatctaagt ctttgacgat ttttacttcc cctgacgaga ctgttgcatt
     6781 cccgtagctt gaaacatcca tgaaatccaa ttccaaatac gtatcgatag aacgaacgat
     6841 atctgccatg aatggtaccg ctccttttaa gacacctact actaggggat tggtgttttg
     6901 gtaaatttct gttaactctt tgcctagttc tttgcaacga acttgtatct cttcttgtga
     6961 gatcaaaatt ctctcaatat ccttttccaa catcaggttc tccttccaat tgcttctttt
     7021 tgatatttgt aaagaagtat gtagtgtatt ttatcagttt cttcacgaat actcaaatat
     7081 gaaaaggcaa aaggtatcaa gctatatata tttcttttgg catctgtaat gatccaagca
     7141 tgctgccttt gtgaattagg tactttctga tcgataaaat atctagatac tttttttgtg
     7201 agattctctg ttaatttaat gcggtctcct gcttttcttt tttcgacata cagttgcaca
     7261 tcagcaggaa tccaaatgtc atgagaaaat agagaccatg aatctgttgc tttgatgtcc
     7321 cctgcatttt caggtttgac cagaccaatc cattctgttt cattcaaata tattccttgt
     7381 tctggaatca gcaacaagcg ctggtcttct gtctggctag ttgcttgttt ttctttttta
     7441 gagagtacaa caaaatgata ctctcttttc agtatccaat tattgccaat actgagttgc
     7501 cattggcttt ttttctgttg ccactggtct aaaatcgact ccatttgttg ttctttcaaa
     7561 ataattcctg tttttgagaa tgctttgtcg aaaaaggagt aaaaaaaata gtatcgttca
     7621 gcatctgtca tttcttcaat catttctgca gaaaatcgaa attgttcctc ccactgttcg
     7681 acttcctttt cgatcaactg tcccatatat ttttgaatga cttggtctgc ccattggatc
     7741 tgttgcgaaa attgctggaa atgtctcaac gcctgtgtat tttcttgttt caattgagga
     7801 actaccaaat gacgaagccg atttctctgg atatctaaca attggtttgt ctgatcctca
     7861 aagtaaacaa gctgcgattc cgctgcataa gtatataggt cttctttgct aaaactcagc
     7921 aacggacgaa tcagtctgcc cgtagcaaat ggttgtactt ctttgattcc agaaaaagta
     7981 tttaactgtc ccccacgaac gattttcatt aacaccgtct cgatttgatc atctccgtga
     8041 tgagcagtca tcaacgtgtc atattgtcct tcttccatca cgcgagtgaa ttcttgataa
     8101 cgaaagttac gagcttctgt ttctatggcg gatttggctg gatcttccca aatacttaca
     8161 tataaaggga gattcttctc ttcacaatat gagcgaagat aggcagcttc ttctgctgat
     8221 tcttctctta attggtgatt gatgtggacg atacctaaag aaaaaccgat ttggcgttgc
     8281 acttgtaaaa gtaaatggag caaaaccatc gaatcaacac cacctgatac cgctgctaaa
     8341 attctcgatc cttgttgcca gaatccttgt tcattcccgt gccgaataaa tgactgctgt
     8401 aatagcggtg cttttttccc gcctttttca acgttcatcc tttccacacc ctttcttcct
     8461 aaaaaaaaga gagcgggaaa acgtctaaaa cgacaattaa cctgccctct gttgatacta
     8521 tgacccaaat agtcgcacta tcattatttg cggcttttta gctgcgacgt ccgccacggc
     8581 caccgcgctt tccttctgtg tttcgcttca atgaagataa gcggtcatcg ctatctttca
     8641 aaaatgaact catcaatgaa tcgaagtctt ctttttgagc tgaaggttga ctgcgagaaa
     8701 aagatttttt ctgtgctgga cgatttcggt tgtcacgggg atctttttgt tgatattttt
     8761 taggacggct ttcttctcca gattcagcag gttgttcctg cgctttccgg atagatagtc
     8821 cgatttttcc atcatcattt actgaaataa ctttaactgt tacttcatct cctacactta
     8881 aaacatcatg gatgtctttt acatagccat ttgacacttc gctaatatga actagacccg
     8941 tttttccagc acctagatca ataaacgcac caaaatttgt gattcctgat acttttcctt
     9001 gcagttttgc tcctacctcg attgacataa aaaaaatgtt cctcctaatt atttcccttt
     9061 tttatattcc tgcttatttt gcagttgtag aactagttga ttgatctgtt tcatttgtcg
     9121 agctgtctgt atttgattct aatataggat aaactttttc accgtctttt gagtaaaaga
     9181 atttgcttct ggcaagttta gctacatact catcatcttt cagcaatgaa acatcttggt
     9241 gcagctgatc aacgtctgcg ttgacttctt tcatttcagt atttgcttcg actctcaatt
     9301 cgttcaattg atgcatcctt tgcatgtcat ggaaaatctg cacacctatt atcgcgaaaa
     9361 tgaccaaagc accaacaaaa attgcagcca gtctgcgtct tcgaaaaatc agttgacgtt
     9421 gttgttgctg aaattcagca tactttttct ttgcatattc cgtatctagc gcagcgattt
     9481 tattgctttt tttcgtcact tggattcgct ccaatctttt tgattgtctt tttcattata
     9541 ccaatatcgt gaaagcttgt ctaatctatt tctctagtgt ttctgtgcga ttttcagaaa
     9601 taatttcgta cattttttgt gcatcttctt ttttggttga ttcatgtaat tccgtaattt
     9661 tcacttccaa cgttttgttg ccaaactgga ctttcaaaat atcgcctact ttgacatcac
     9721 tggaagattt cgccaattta ccgttgattt gaattcttcc tttatcagct acttcttttg
     9781 ctactgatcg tcttttgatg attcgggaaa tttttaaaaa tttatctaat cgcatatctt
     9841 attcctcact ttttaatttt tttatttaat cgcaggattt tcttaccaaa aggcaacatt
     9901 agccattctc gtactgtaaa cagacgaaga cgaatcactg cataaatgaa cacagaagcc
     9961 ccgatcccaa ctcctatcac gctgacaaaa aaagcagtgg aacgatgagt tacaggacca
    10021 aatactatat tcaatacacc ataatacagc agcagtacta ctgtcataca aagaaggctg
    10081 aacatcaact tccgtccaaa atgccgttct ttccaaaaag catttagctc ttttgtttca
    10141 attcgaacaa aataccaaag cgtcacacct aatcctaaaa gggtagacag gcttgcacct
    10201 gctgttccta ataaaccggt cagaaaagaa gtagtcaatc cttttaccaa tagtccccat
    10261 cctgcgcctt ttaaagaagg gcgaaacgag ttcttgcttt gagcaatact ttgataagct
    10321 tggatgactg cagtgaaagc aatggaaaaa acaaataata caagcgctgc atttcccgca
    10381 taatctttaa acaacgcata attgatataa gggagcagca aggctaatcc caaagacgca
    10441 gctaatgcta aagcggtcgt caatctaaga tagattttgg ctgtctgtaa aaactgacga
    10501 tagatccggt tggtcaaatg acgagtcaac gcaggcagga atgtcgcact taatgcagtg
    10561 gcaatcacta aacctaattg tactaaaggc tgacctcgat catagacccc ttttgctatc
    10621 ttcgcaccgt attctgacag tccgtaaact tccaaagcat tttttacaaa gaaagagtcg
    10681 atcagctgga aaaagataag caatccacta taaatcgaaa ccagtccgcc ttctaccaga
    10741 aaacgccgga tcaagggctt tgacggctgt ttgaacaatg gaaaccgtcg gaagcttaac
    10801 gctcctccat gaatcttttg ttcatagtag tgaaggatcc catatgcgca taaaccgcca
    10861 aaaacagctc cactcattgc tgccgtccct gtttggtaca ccgtccatcc aagctttcta
    10921 aaagcaaacg cactacagac aatcacaaaa acacgaacga actgctcaac tacttgagaa
    10981 acagcagttg gttccatcaa gaatcttcct tggaaattcc ctcgataaaa agatagacca
    11041 ggcatcagca aaaaggtaaa ggaaactact tggatcagcg gtttcaattg tatgtctccc
    11101 atcatccaag caatccagct gctaaatgca aagacaaaca accacaaaaa gacacctgtc
    11161 caaaatacta agggataaag attttgcaaa gcttgctttc gctcgaaagg attctttctt
    11221 tctgcaacgt actttgatat gaactgcgga agaccggata aagctaaagt cattgctatc
    11281 ccataaattg gataaacttg ctgataaaca taaaaacctt cgtcgccgac taagttttgg
    11341 aggggaattc ggtaaagggc acttaatact tttgctatga aagaagcaat cgtcaataca
    11401 aatgctcctt gcatgacgcg ccgcatttct ttatgatcca aatcattgct ccttcctacg
    11461 ctgacagata tttttgctca cgcaacgctt tgacaaactt ttggatttcc tgaagccata
    11521 ctgcttcttc catttggttg gggatcgtca agcgtatcac catctgttct ttttcaacgc
    11581 ctagactggc ttttagtttt gtcgctgtca gtgcttcgaa taactgttcc acactatagc
    11641 gttttgttcc aactttactt agagtaaaga ctatatgcgc ctcctgtttc cttatcgttt
    11701 ctaggagcgc tcgatcgcca tccattttga tctgcccaat tgtcaagaga tgtgctactt
    11761 cttctggata ttctccaaaa cggtcgagca gatcatcttc tagttcatct agcatttctt
    11821 gcgaatcaag ttcacgaatt cgtttataga tctcgatttt ctgccgttga tctgccacgt
    11881 atgtttcagg tagatacgca tcgacaccga ggtcgatctc aacagtggtc ttctccactt
    11941 gactattctt tccttgttta cgagaaacag cttcgcttag catttgagaa tacatatcga
    12001 aaccgactgc atcaataaat ccatgctgct gagcacctaa gagattacct gcaccgcgaa
    12061 tggaaagatc ccgcatcgca atcttgaatc cagatcccag ttctgtaaaa tctttgattg
    12121 cctgaagacg tttttcactg acttcgttaa ggatcttctg ctgctcatac ataaagtaag
    12181 catatgcgac acggctgctt cttcctaccc gccctcgcaa ttggtataac gtggataatc
    12241 ccatataatc agcgttctca acgaacaacg tatttgcgtt cgggatatct actcctgttt
    12301 caatgatggt tgtagtgaca agaacatcat attgaccttc aataaattca aaaagcgtat
    12361 tttctaattg cgcctctgtc atttgcccat gagcataacc gatccgtgct tccggaacga
    12421 gttcttggat ttcttctact ttttgttcga tcgtttctac ccgattatat aaatagaata
    12481 cttgtccacc tcgtcccatt tctcgatgga ttgcttcgcg aatagcccca ggattttttt
    12541 ccataacata cgtttggatc ggataacgat tcgcaggcgg tgtttcgatt actgaaagat
    12601 cccgcacgcc taacatcgac atatgcaaag ttctcggaat cggtgttgca gtcagagtca
    12661 aaacatccac ttgtgcacgt aactgtttca atcgttcctt atgttttaca ccaaaacgtt
    12721 gttcttcgtc aatgatcagt agacccaaat cgctaaattc aatatctttt gacaaaattc
    12781 ggtgggttcc tacaacaata tcaacttgac ctgtacgcag ttgttcgatg gtttcttttt
    12841 gttgctttct tgttctgaaa cgacttaata gcccgatatt tacggggaaa ccttcaaatc
    12901 tttccagcat cgtttcatag tgctgctgtg ccaagatcgt tgttggaact aaaaaggcta
    12961 cttgtttgct ttcctttatc gctttaaagg ccgctcgaag cgctacttct gttttcccat
    13021 aaccaacatc acctacgagt agtcgatcca ttggtttttc tttttccata tcccgtttga
    13081 tctccgctgc acttctcaac tggtcatcag tttcagagta ggggaaggca ttttcgaatt
    13141 ctttttggta ggcgtcatca ggtccaaaag catatccttt ttctgactct cttgcggcat
    13201 ataatttgat tagatcgtca gcgatatcct cgatttttga agagactttt cgcttggttt
    13261 tcgtccattc acttccgcct agcttattga tgcgaggcgc tttggcttca gaggcgacat
    13321 atttttggat cagattcagt tgagtaacag gaataaacag tttgtcgtcg ttttgataca
    13381 aaatagtcat atagtcttgg tgaacgccat ctacttctag cgtttccatc ccaatgtatt
    13441 tcccgatacc atgattggca tggacaacgt aatctcctgc ttttaactca ttatagcttt
    13501 tcaatcgctc tgcgtttgtt actgtttgac gtctcgtttg ttttttcgtc gttttttgga
    13561 aaatttcttt ttctgtgata gctactattt tttcttgagg aagttcaaaa ccagcctgca
    13621 aagctccttc aactaattgt acctgtcctc tgatcaatgc atttttttct gtttctacta
    13681 ctaatatgtc ttcatctcga agcatttgtt ctgctttgcg tattctgtcc tttgtcggaa
    13741 taaagatcac aacagtctgt ttttgcttgc gccaacggtc catttctgtt ttcagcaatg
    13801 gcatttgtcc gaagaactgt tgcatcgtcc gatattgaaa attatggatt gcttggaaac
    13861 gaagatttcc catccctttt tggaagagtg agaaaaaggt tgtggtgaac ttttccttct
    13921 gcacgatttt ttgtatatct gctgaaaatg tctgctcaga aaacacccgc aattcgctga
    13981 ttttttgagt ctgccattct gccgcttccc tttcgatttc ccgatttgtt tccatgattc
    14041 gctgataatc atcaacgact agtaaactat ttgaagggaa ataatccaag atcgtcacag
    14101 attcttggta aagaagatct gagtagaatt ttgcttcttc tgtcgggaca cctgcttgcc
    14161 agcttgaagt cagctgaccg aagtaatctt gcaaaaactc gcggtctgct tcttctgtgg
    14221 tcgcagcaag ccgtttttct agcatttttt gcatttgctc tattccatgt tctagatcct
    14281 ctggtccgta gacttgttca ctcgttgggg aaacccagat ttcttccgtt ttttcgattg
    14341 agcgttgtgt ttctgcttca aaataccgca tagaatctat ctctacatca aatagttcta
    14401 cacgaacagg ataggttgtc gttaacgggt aaatatcgat aatgcttcct cgaatactga
    14461 attctcccgg ttttgcgacc atctcttctc gtacatagcc catcaaagtc aattggcgag
    14521 gaagattttc cacgtctatc tcatcaccaa tatgccaatt caattgcgcc tgtagccaag
    14581 ttttcttatt cggcagtcgt ttgtgcaaag atgcgactgg caacaaatag atacctgctt
    14641 ttttttcttg aagtgcgtta agcgttgcaa ctctctcgcc tcgcgcttct ggagaagaaa
    14701 aggccatttc agcagaaagc acttcatcca ctggaaataa atggatctga tcttgcggaa
    14761 tcacatgttg cagatcttct gctaactgat tcgcataata caaatttggc atcagaataa
    14821 tcatgttttt atttgttttc ttaaacgctc gagcaaatag aagtgttttc gctgagcccg
    14881 ctaatcctgt gattagttgt ctaggcattt tttcttctaa attgctgacc cattctttta
    14941 cttgcggatc ttttccgact aattgaataa tatccattcc ttttcctcct tcctcaattg
    15001 aatcgattca tcgcatccac gaacgaatgc ccttctatga gatattcgac tgctttaaca
    15061 gattgacaca tgctttcttc tatcaaaggc tgattttctt tgctgaaagg actaagcaca
    15121 tgctggacta ctgttttttt cccttctggc ctgccgattc ccaccttgat tcgatcaaag
    15181 acattcgtat tcaaatggga aataatactt ttgataccgt tatgcccgcc ggcacttccc
    15241 ttttgacgga gccggatttt tcccactgcc agatccaaat catcgtagat tacgactaat
    15301 tcctctggat aaatcccaaa ataagtcatt agtgggccga ctgcacgacc agattcattc
    15361 atgaatgttt gtggtttgac taaaaggatc ttttctccat tatgaaaaaa ttcggcaact
    15421 tctgcttcga acggattttt tttgaaagta gcttgatgct cgcgtgcaat tttatccacg
    15481 accataaagc cgatattgtg ttttgtatat tggtatttgg tacctgggtt tcctaacccg
    15541 acgatcattt tcatttttgt tttccaccta attctaaaaa gcagcacaaa aggctggact
    15601 gctaattagc ggtccagtcc atcgagcttt tttctttttt acacttgtca atgcatagta
    15661 taacacaaaa caaggcgttt cggctaaaag agaatcgtta ataaatgata aagaaattaa
    15721 aaatttcttc aaagtttaat gaaataattg atatataaaa atagtgaaat aacgcacatg
    15781 taagctttta catataatac aaccttgtta tctgttatat tttatagcca aatagcgtga
    15841 catggggctt gagaaatgtt agcataagca tgggaaaaaa caaatcaatg gaaggattga
    15901 tactaaatga ctgcagctgc agaaaaaaaa gatcatcaaa aagttattct tgttggagac
    15961 ggcgccgtag gttctagcta tgcctttgct ctagttactc aaaatatcgc tcaagaagta
    16021 ggtattgtgg atatcaatac tgctaaaact gaaggagacg caattgactt gtctcacgct
    16081 ttggcattta cttcacctaa aaaaatctat tctgctactt acgcagacgc acatgatgct
    16141 gacttagttg ttattacagc tggcgcacca caaaaaccag gtgaaactcg tttagactta
    16201 gtaaacaaaa acctaaaaat caaccgcgat gttgtcacac aaatcgttga atcaggattt
    16261 aacggtatct ttttagttgc cgctaaccca gtagatattt tgacttactc aacttggaaa
    16321 ttctcaggtt tccctaaaga acgcgtaatc ggttcaggta cctctcttga ctcagctcgt
    16381 ttccgtcaag cacttgcgga attagttgac gttgatgcac gtaacgtcca tgcttacatc
    16441 ttaggtgaac acggagattc agaattccca gtttggtcac atgcaaatgt tgccggcttg
    16501 caaatctatg aatgggtaaa aaacaaccca gaaatcgatg aagaagctat ggttaaccta
    16561 ttctttggcg tacgtgacgc tgcttacaca attatcgaga aaaaaggcgc tactttctat
    16621 ggtatcgcag tagcattagc acgtatcact cgtgccatct tagatgatga aaatgctgtt
    16681 cttcctttat cagtattcat gaatggcgaa tatggattga acgacatcta cattggtgca
    16741 cctgctgtta tcaaccgtca aggtatccaa aaagtaatcg aaattccttt aagtgacagc
    16801 gagcaagacc gtatggctgc ttctgctaag caattaaaag aaattctaga tgaagctttt
    16861 gctaaactag acgctgaata atctttgaac atgacggaaa acatctattc ggtgttttcc
    16921 gtttttttat tttatcaccc ttatggtttc taaatttttt cataaagtag agataacctt
    16981 cgtcttttcc tctaaattat gctatcatga gaccgttcca ccctaaactt ttacttaagg
    17041 agttgtcgat atgacaagat cttcgcatcg aaaaaaatcg aaacatgttg ctttgctttc
    17101 agttcttggt gttctcttag tgctgatcag cttttatacc tatcgcagta catactacac
    17161 aaagcatttt ttgccgaata cagaaataaa tggaataaat gtcagcaatc taacagtcga
    17221 aaaagcaaat gaaaaattaa aagaagctta ttctgataaa aaactggcta ttaaagaaga
    17281 tggaaaagtc tggcaggaag taccaaaatc cgagctcggc tacaaagatg atttcacttc
    17341 ggagctttct ccgatcctaa atgaacaaaa cggatggact tggggaatga cttatgtctc
    17401 agcggcagaa aaacaagaaa tagatcctgt cagtcaaaac caccaaaaac tagacactac
    17461 cgttcagaca ttgacaacta agctgactga tttgaacaaa gaccggacac ctactactga
    17521 tgcaacgatc gaaaaagcag gtgactcttt cgttatcaag cctgaagtca atggaaatac
    17581 gatcgacgtc gatgctgcgg tcaaacaatt aaaaagtgcg gtaaactctg gtaaagatac
    17641 aattgaattg actgaattca aagaaaaacc aaaagtaaca tctgaagaca gctcactgaa
    17701 agaacaactc gcatccatga atgctatcgc aaatgtaaaa gctacttaca gtataaacgg
    17761 tgagactttc cagatcccat caagtgatat tatgagttgg ctgacctaca atgatggaaa
    17821 agtcgattta gatacagaac aagtacgtca atatgtaaca gatttaggca caaagtataa
    17881 cacaagtacg aatgacacaa aatttaaaag tacaaaaaga ggagaagtta cggtcccagt
    17941 tggcacttac agttggacga tccaaacaga cagtgagaca gaagcattga aaaaagcgat
    18001 tctggcaggc caagatttta ctcgttcacc cattgtgcaa ggtggcacaa ccgctgatca
    18061 tccattgatc gaagatacgt atatcgaagt tgatctggag aaccagcata tgtggtatta
    18121 caaagacgga aaagtggccc ttgaaacaga tatcgtttcc ggaaagccaa ctacacctac
    18181 acctgctgga gtcttctatg tttggaataa agaagaagat gcaactttaa aaggaacaaa
    18241 tgacgatgga acaccttacg agagtccggt aaactactgg atgccgattg actggacagg
    18301 tgttggtatc cacgattcag actggcaacc tgaatacggt ggagacttat ggaaaacccg
    18361 cggttcacac ggctgcatca acaccccacc aagtgtaatg aaggaattat tcgggatggt
    18421 cgaaaaagga acgcctgtat tggtcttcta acaaaaataa tatataaaaa gaaagcaccc
    18481 gtacgattgg tacaggtgct ttctttttat tatctaacaa tgtgctactt acatttcagc
    18541 agcagtaaag acttctgaaa catcatcatc gtcttctagc ttatctacta gacgttccaa
    18601 ttgctctttt tgttcctcat ttaaatcaat cgtcgtttgt ggaatcatcg tcaattctgc
    18661 ttgcgccaaa gtgaactctt tttccaactc atctcgtaca gcagcaaaat cttctggtgc
    18721 agtataaatc tcgaatactt caggagaagt ttgcagatct tctgcccccg cctctaatac
    18781 atcctcaaac atcgtctctt catcaacagt caacccatcg cgttcaatag cgatatatcc
    18841 tttgcggtca aacaagtaac tgactgaacc cgtttctccc agtgatccac cgtttcgagt
    18901 aaaagctact cggacatttg tcgctgtccg attacggtta tctgtcaatg catggactaa
    18961 gattcctact ccacccggac catatccttc atacgtgatt tcatcgtaat tcgtttcatc
    19021 tacggcactc gtagcctttt taatcgcccg ttccacattg tcgttaggca tatttgcagc
    19081 ttttgctttg tccacaacaa gccgcaaagc tggattcata gatggatcag gaccgcctgc
    19141 tttagctgcc atataaattt cacgagaaag tttctgaaag actttccctc gttttgcatc
    19201 ttgggcattt tttcttcctt ggatattatg ccatttacta tgtcctgaca tgatttttcc
    19261 tcccttgttc ttttgagaat acacttctat tctagtacta tttcacaaaa aaacaaagcc
    19321 acagccaatc tataaacgct tcttgaatgc cgcaaaaagc acacataata tcacagcagt
    19381 cagagcaccg atcgaatcca acatgacatc ttggaacata ggtgttcgtc caccggtcat
    19441 catctgatgg aattcgtcca aacctgcgta ccctgttgct gaaagccaag caaggatcgc
    19501 tgtcagccac caaattttta gcttggggaa aacacctaaa aacaagctgc ctcccaaaat
    19561 aaaataggta ccgaaatgtg cgcccttcct taaaaagaac tctaaaaatt tggcgtagcc
    19621 cagatggttg atactcacca tacttccacc ataattaaaa cgtatacccg ctaatgtgtc
    19681 tttaaatggc tgattaggca gccagctttc taatcgcgaa acttgtgatt gctgtccgta
    19741 agtttgagaa gaactgtaaa ataagattat aacgactatc agtgcgatta ctaaataaat
    19801 atttccgttt ttcatttgtt tttttgaaga attcataagt cacctctgta ttttatctta
    19861 gacttttttt gaagaaacaa caatcatttt catagagact tcttccataa aaaagtgcta
    19921 tacttaattt tgtattacaa tatgaaggga gttttttcat gagttattca ttaaattgga
    19981 atctagattc tattttttcc ggaggcagtc attctgatgc tttgaaccaa cggatgaagc
    20041 aattggaaga ccaaatgaac gagtattatc accgcgtaac aaaatggtca cctagttctg
    20101 acaatgcaga gcagttaaac gctattcttc aattgcaaga aacgattacg aatggtttta
    20161 cccaatgcag cagctatatt actgcattgc tttctgccaa tgtcaatgat tcagatgcaa
    20221 aggtcttatc aggcaagctt tacgcaatgc tgcctcgtct gcaatcagcc gaaactgtat
    20281 tgtcaaagaa atttgcagaa atatctgaca atgattggaa tcagatgctt tctcaatctt
    20341 cgtttgaaac aattgctttt cgtttaaacg agattcgtcg tgatggctca caattattat
    20401 cagaagctga agaaaacatt ataaatacgc tttctttaga tggtctaaat gcttggagca
    20461 gtcattatga tacgattgta gccagcattt cgattccctt cgaacaagat ggacaagtag
    20521 tcgaattatc tgctggacaa gccttcaata agatgatggg cgatccagat ccaaaagtca
    20581 gagaaacact tttcgcagca tgggaagaag cctggaaaga aaaggcaccc ctcttcacgg
    20641 atactttgaa tcatttagat ggttttagac tttctgacta tgaacttcat ggtgtcacag
    20701 attttctaca aaaaccatta gagtacaatc ggttgaagaa agaaacattg tctgtcatgt
    20761 gggatacaat ccagaaaaac aaacaacccc ttgttaatta cttgactaga aaagctaatc
    20821 ttttcggtaa agaaaaaatg gaatggcaag atcaagatgc acctattatt ttaggcgatt
    20881 tgaaagaaaa aacctttagt tttgacgaag cagcagcatt tattatcgaa aactttaaca
    20941 aattcagtcc aaaaatggct aaatttgccc aatctgcttt tgaaaagagc tggatcgaag
    21001 cagaagatcg tcctgggaaa cgtcctggtg gttattgtac tgagttacca gaaacaaaag
    21061 aatcaaggat ctttatgacc tacagtaatt ctgttaatga ggttgcgaca cttgctcatg
    21121 aactaggaca tgctttccat agcagcacga tgtgggattt accttcttta aatcgcgaat
    21181 atgccatgaa tgtcgcagaa acagcaagta cattcgcaga actgatcgta gccgatgcta
    21241 cattaaaagt cgcaaaaaca aaagaagaaa aaatcaattt gctagataca aaactgcaaa
    21301 atgctttagc tatgttcatg aatatccatt cccgattcat ttttgaaaat cgattctatg
    21361 aagcacggca agaaggatta gtctcagaag ataagatcac agagatgatg gttgaggcgc
    21421 aaaaagaagg ctatcaagga gctttagcta cttaccatcc atatttctgg gctgcaaaat
    21481 tgcatttctt tattgatgac gtaccgtttt ataacttccc atacacattt ggctacctct
    21541 ttagtttggg catctatgct tacgccaacc aaaagggagc aaattttgaa gaagaatata
    21601 tccatttact acgcgatact gcctcaatga cgacggaaga tcttgcgaag aaacatttga
    21661 atgtcgatct tacccaacca gatttttggc aagcaggtat cgacatggtg actgaagata
    21721 tccaaacgtt catggaattg acagaagaat ttatctgatt tctattttat cacaataaaa
    21781 aaagagttag tccagaatgg gctaactctt tttgattaat cattcttaca tgctgacagc
    21841 aaagtcaggg gcaaaccatt gaactgaacc aacttttacg ctttcgcctg gttgaggagc
    21901 atggatatat tgtcctccgc ctaaagcaat cgctacgtgg taagttccgc cttgtgaccc
    21961 ccagaataac aagtctccag cttttgcttg agaaactgaa atttttgttc cagctgattc
    22021 ttgaggaact gtccaaccac cgatgtcacg accagttact tgcatgtaaa catagcgtgt
    22081 gaaccctgag cagtcaaagc cacttggatc tttaccgccc caaacatatg gagtaccaat
    22141 atatttgtaa gcttctgcta cgattgctgc accattcaca cttccagaag gagctggagc
    22201 aggtgttaca ggttttggtt gttcaggtgt tggtgttggc gttggagccg gagtactact
    22261 tcctgtgccg tttcctgtat caacactttg atctgttgat ggtgttgtag gcgcaggtgt
    22321 tgttgattct tctgttgttg aagattcagg cactgttgtt gattcttctg ttgctgaaga
    22381 ttcaggcact gttgttgatt cttctgttgc tgaagattca ggcgctgttg ttgattcttc
    22441 tgttgctgaa gattcaggcg ctgttgattc ttctgttgaa gattcaggtg ttgttgattc
    22501 ttctgttgct gaagattgtg tagctgatga tgattcttca gtcattgaag attgtgtagc
    22561 tgatgattct tctgttgctg agctttgtgc agtagatgaa gcttgagtag cttgtgcttc
    22621 agcctctgct tgttcgcgtg cttctttttc agctttttct tgtgcagctt gttgacgagc
    22681 ttgttctgct agacgttgtt gttcacgaat acgagcttgc tctgcttcag cttctgcttt
    22741 ttgacggttc aagtcagctt tcttgtcttc agcagttgct tgttcagcag ctaatgaagt
    22801 tttcagtacg ttcaaatcag cttgtttaga taaaagatcg cctttttgag attccaaagc
    22861 agcttggttt tcagctaatt cttttagttt agcatcgttt tcagcttttt tgtcttctac
    22921 agctttttta tcttgttttt gttgttccat caagtcgttg tttgctttta ccatcgttgt
    22981 cattgcttga acgcgtccaa tagcatctgc taatgaatca gcattcaata cagcatcgat
    23041 gtagtttgag cttgtgttgc ttacttgtgc ttcgcgtgct tgtttttgga ttgtatcttc
    23101 acgtttttca atacgttctt gtaaatctgc aatgtctttt actaattgtg cagattcttg
    23161 acgcaatgta tcttgttttg ctaataggtc ttgtgcttgt gtgttgattt cagaaacttg
    23221 gctttctaag gcatcgattt gtgattgtgc atctgcttgt tgatttttca agtctgcaat
    23281 cttttgatct tgctgttgta tctgagaatc aaaatcgtcc gccgcggcag cgatcggtga
    23341 tgctacggca gtcaaggtca ttgaacatac cattactgct gatattaaac tctttttcac
    23401 tcttcattcc tccgactggc ttaatttaat aaatatctaa atatatttaa gtctttctta
    23461 acgatataaa aattgtacca tagcagtgtg tcaacgatat aatagttatg ttacaaaaat
    23521 gtttcaaaac aacttttaaa aatgacaaaa ctgttcgttt cagaaaatga tcatgaaaaa
    23581 gccgcgttaa gtctagcatt tcttaagata acaacgattc tttttcgctt tcgacctaaa
    23641 aataaaaaaa gaaattattc atcactgaat aatttcttaa agggaaaaat gaataataca
    23701 aatataatca tgttcaacag cagtgtaggt cctaaaaacc ttgtaataaa atacgtgtta
    23761 tttactactg ctaatttaaa aatcaactgg accaccatcg taaacaattc ataacccgtg
    23821 acaaagatga tcatactgaa aaattcagta aagatgttga catggataac gtctttcatt
    23881 acatacatca accatacaat taaagggaga gctactgcat agataccgat aacgccaata
    23941 tagtaagcgt catagatagc cccgagaaca atcgtggtaa tcagtaaata acgtttttca
    24001 aacgccatac tgcaacataa taatgccaat atcagaaagt gggcatttga catataggtg
    24061 cctttcgacc actcccctag cattcttgtt aaatgaccat ctatcaacat caaaaagaac
    24121 aaaacgattg gcagatagta cttcattgtt tctttcttca acatggacta atcctccgct
    24181 agccgttgga caatcgtcac aactgacacg tcatacattt ctgcataagg tttcacataa
    24241 acttcccgat ccagcccata actatctggt ttcgttttaa taaccgtacc gatcggcaag
    24301 ttagctggag aattccctcc aagaccggat gtctggacaa catccccttc tttgatatcc
    24361 gtatcaccag ttaattgggt caccactaag gcatggagct tttcatcata gtttttaagc
    24421 aaaccaaacg cttcgccatt agccgaagat acccgtactg gaaaatgatt ggagctttca
    24481 ttagaggatg acagtaattc gattttagac gaagccgtat tgacctcgat cactcggcca
    24541 atcaatcctt tttgcgacat gactgccata tttgcttcga taccgtcttt agaacccttg
    24601 tctacgatca gcatgtcctg ccaactgtca ggcgaacgag taatgacatt tgccgttact
    24661 ttttcgtaac tagtcagcgt ctgattcaac tcaagttctt ttttcaacgc ttcgatttct
    24721 ctctcgtaat tcttgttttg ctgtgaaacg tcatcatagc tatcgatttt ttcttttagc
    24781 cgctcatttt cggcataagt tgaaaaaaga ttatggacag aatctactcc atttcgtacc
    24841 caccgtactg gggcagagat tgttttatca accattgcaa cactgtcatt aacgatggat
    24901 tggaagaaat tgggctttcc ttcattcgct cgctgcgcag cggtcacact tagtatcgtc
    24961 accacgatga taacaataac caaggtaata ataatatttt tattaggatt gaacttcttc
    25021 acaaagacac ctcgaattaa ttagactctt gtgttcaatt ttatcactaa atgccgtgaa
    25081 acaaaaggta agttgctcct cttaagcttt tttttagcaa ataatagatt ctgtcatttt
    25141 tagcctgaaa gacaaatttt tttatttgtt tatggatcaa tctttgcttt gaataactgc
    25201 aatcatacca tctgaaaaag aaaattctgc ggtatctgaa agaaattcgt tacttgcata
    25261 agatgtcgga tattctactt ctttattttc caatacatac ttgctgcctt ttaatgtaag
    25321 attcgagacg ggagtcaagc agcaataagc taaatacttc atatctggta tctgtcttac
    25381 tatatgctct ccaggcaaat aataagacaa ataattttga caatctctga taataagctg
    25441 atgggcaaag ggtttgaagc gaggttccat acctaaccag agattggcta aaagatgatc
    25501 caatcttccg cccgtagctc caatcaaagt gacttccgct tgcgggtaac gttcaaatgt
    25561 ttttaagata cctaactgtg tatctgtatc gtctttttct gcaggggaac gggtatactc
    25621 tttggctttt gcaaatactt ccttctgctc ttcgttcgac aatgaatcaa aatcacccac
    25681 tgccagatct actgggtagc ctgcttgaag caggtacagc gcaccacggt ctatcccgac
    25741 ataggcatcg tatttggaca gcagagactg cggccagttt tgcggattac ctccagcagc
    25801 taataaaatt ttcatttagt ctaatgcctc ttttaatcgt tcgatttgtc cgactggatt
    25861 ttcggctcca tagatatatg atcctgcaac gaagacatcc gcccctgcat ctttacagat
    25921 ttttgctgtt tctggaacga ttccgccatc tacttcgatt gcataagtcc aattgttctc
    25981 tctacgtaga tcagcgagtt ccgtaatttt ttctactgtt tcttcaataa agctttgtcc
    26041 gccgaaccca ggattgacag tcatgactag tacttgatca gccaaaggca aaacatgttt
    26101 gatcatggag actggtgttc ctggattgat aactactcca gctttaactc ctaagctttt
    26161 tgtcatttga agtgcccgat gaatatgagg agttgcttcg acgtggacag taatgatatc
    26221 tgctccagct tttgcaaatg ctggaatgta gttttctgga tcaacgatca tcagatggac
    26281 gtctaacggt aatttagtaa caggacggat tgcctgaaca atatttggcc ctaatgtgat
    26341 atttgggaca aatcggccat ccattacatc tacgtggatg taatctgcgc ctcctttttc
    26401 cactaactca atgtctcttt gtaaattggc aaaatctgca ctcaaaatgg atggtgctat
    26461 cttcattctt gtttcctcct attttcgttt accttctttt tttgcataca ttggttttct
    26521 tttttcgatt tcttgtaaga attgcaaata attgtcgtaa cgtgtctgtg cgataccacc
    26581 tgtttctact ctatgtttta cttcgcagcc aggttcttta gcatgcatgc actctctaaa
    26641 tttgcaatgg acagaagctt ctacaaattc aggaaattgt ctcggcagtt ctcctgcttc
    26701 aattgtcaaa aagtcaatcg agctaaatcc tggtgtatct gctactaacc cgccatagat
    26761 aggaatcagt tccacatgac gtgttgtatg ccgtcctctt ccaagtgcat cagaaatctc
    26821 tcctgttgct aaattcagtt ccggtgatat ttgattcagt agcgtcgatt ttcctgctcc
    26881 agactgaccc ataaagaccg atagccgttc tgggaagtaa cggatcagtt ctgtcgtatc
    26941 acccgtatga ttgggtacaa tcaccggata gcctatttta gtataggttt gcttgatctc
    27001 ttcgattttt tctgggtcgt ctgacaagtc ggtctttgtc aaaaaaataa tcggttcgat
    27061 cgactcgtat tccaaggtaa ctagaaagcg gtcaagtaga ttataagaaa atgcaggctc
    27121 tacgatactt gtgacaatga ttccaagatc aacatttgtg actggtgggc gaactaattg
    27181 attttttctc ggcaagattt ccagaagata gccgtctgtt tgattttcac tctcaaagat
    27241 cacctgatct ccgacaagcg gcgtgatttt ccgattccgg aaatttcctc ttcctcttgt
    27301 ttgataggtc tctccgtttg cgtatacata gtaaaaaccg ctgattgctt ttctgatttg
    27361 tcctttgata agtgccacct ccgtttttgt ttattttacc atacgaagag gaaagtaggt
    27421 gttattccat gcaaacttct caaagtatat ggcttgaaaa ttgatcaaaa gagatagaaa
    27481 cacaaaaaag agaatgcaac aaatcaatgt cgcattccct tctgatatat tttattcagc
    27541 acttattttg aactttctga ggtgctagct gatgaacttg tggagctgga agattcttct
    27601 tttgaagagt tgcttgtgct actggagcta cttgagctgc tagaactgtc tgaagagtct
    27661 ttttcagaac tagaagacgc tggatctggt cctttagagt aaattacgtt cacaaccatt
    27721 cctttggtaa ttgtcgttcc tgcccctggt actgtacgaa tcactttatc tttatccacc
    27781 gtgtttgaat attcggaagt ctcatgcccg atatattctg caccgacact agctaaataa
    27841 ctttgtgctt cgctttttgt atagccggag atatcattca atttaggctc tgttccttgg
    27901 cttacgacaa aattgatgta agtttcttct gctacaactt cacccgaggc tggatcctga
    27961 ctgatgatcg aacctgatgg tacagtgtcg ctatagtcat aagtagcact aatattattg
    28021 atattgaatc cttgtgtaac aagccaatta cgaacagttt cgtagttttc tcctacatga
    28081 ctactcaaag tcactgtttc tggtcctttg ctgactgtga aagtaatcgt gtctgtttta
    28141 ggatcgactt ctgtatcttc tgatggagat tgcgaaatga tcattccgct ttctacctct
    28201 gaactgaatt cctcttcttt ttgaatcaag tcagcagaaa accctaattc tttcaaactt
    28261 gcttcggcat cttcataact tttaccactg taatcatcta atttgatttt tttcgtgccg
    28321 ctactaatat acagagtgac ttcgcgtttt tgcttgactg tcgttcccgc tgccgggtct
    28381 gtcttcacta cattaccttc ttcgattttg tcatctgcaa tttcttctgt ttttgttgct
    28441 acttctaatt tcgcttttgc gagtgtttct ctagcagaag cttcagaaag atcagaaaca
    28501 tcaggaacaa caacttctcc tcgcccgcca gaagcaaaga atacagctgt tccgattcct
    28561 aaaacagcaa gaatagctaa taggatcagc caccattttt tctgtccttt ctttttagga
    28621 tcaggcagaa cttcttcctc ctctggtaag gtttcgtttt ctggcttgtc ttttggcaga
    28681 ggcatagatt tgaatgaatc aggcattggt gcatcttccg taaggggtgt gataactttt
    28741 gtttcattat ccatcacatg aggctgccat ttttcttcgt ttgcacgagc tggtgataat
    28801 actgtagaaa gatcatcaga catttcctcg gctgacttat aacggtctgc aggttcttta
    28861 gccgttgccc gtaaaatcac gttttccagt gcttgtggca catttggatc aagtgctttt
    28921 atagatggaa gatcatcttg aaaatgtttt aaagcaattg ttacagcaga ttcaccatca
    28981 aacggtacgc tacccgtcaa catctcataa aggatgatcc ctaaagcata aatatctgac
    29041 tgcttcgttg ccatacttcc tctggcctgc tcaggggaca gataatgaac agatcctagc
    29101 atcgtattcg tctgcgtgat cgatgtttca gataaagcga tagcaatccc gaaatccgtg
    29161 atttttacaa caccttcatt atcgatcagt acgttttggg gtttcaaatc tcggtggatg
    29221 atttgatggt tatgagctaa agaaatagca gaaaggatct gctgcataat atttactgct
    29281 gtttcataag gaacaggata atgtgtttgg atatatcgtt tcaagtccat tcctttgacg
    29341 tattccatca ccagatactg catgttgtct tcttcaccaa catcgtaaac actaacaatg
    29401 tttggatgaa cgagttctgt tgctgctaaa gcttctctct gaaatcgtcg aatcgcagtc
    29461 tggtcatttt gaaagtcaaa tcgtaagact ttaactgcta catcacgatc caagattaaa
    29521 tcgtgcgcca aaaatacatt tgccatacca ccgctaccga tatttccagt gatttgataa
    29581 cggccattta attttttgcc caactcgatc atacactgtc ctccttatat tcgataagaa
    29641 gaacggtgat attatccgct ccgcctgctt cattggcacg atcaatcagc tcagttactt
    29701 tatgagacaa tgggccttct tgattgatga tcgttcctat ttcttcttca gacagcatgt
    29761 tcgtcaagcc atctgaacat aacagcagtc gatcttttag ttgccaaaga tgagtagaaa
    29821 catctacttc gactgttcca ggcatgccga cagaacgtgt taaaatattt ttccttggat
    29881 ggttcactgc catttcttcg ctgatttctc ctgatttgac tagttcattg actaaagaat
    29941 gatcctccgt caattgcatg attttatctt cacgaatcaa ataagctctg ctatccccaa
    30001 cattggcaat cgtgaaccag tcatctgaca gagcggccgc tacgattgtt gttcccatac
    30061 cgttatattc cggctgttct tgtccacgct gataaatcag catattttct tcttgaatcg
    30121 attgaatgaa ccattgggcg attttttcat cgtcaacagc ttcttgtttt tcccaagctt
    30181 cgcctagatt cgttaccgcc atctgactgg caatgtctcc ggcacgatga ccacccatcc
    30241 cgtctgctaa tacagcaagc ttgagacctg aacggctggt aaatacactg gcatagtctt
    30301 gatttgtgtt acgtcgtctt cctacatctg attgatattc aatctgcatt ttttcacctc
    30361 attacttttt gcgcaaacaa caaatgaaaa atccatctgt gtaaaactga tgcggataca
    30421 aagtcaacat tttctcttcg attgatgatt gtagcatagg attggtcaaa acatcaatcc
    30481 tctcaaaatc agggtgtctt tccaagaaag cagaaacaac gtcttggttt tcttctttta
    30541 agatcgtaca agtgctataa gtcagaatac cggaagactt taaagttggt gcgcagcttt
    30601 ctaaaatttc caattggatt tttggtaaat tcgctaattc atccggctgt tttttataac
    30661 gaatatctgg ttttcgccga agcagcccaa gacctgaaca aggcgcgtct actaaaatcc
    30721 gatcaaactg ctctgcttcg aactgtttac tgacttctct agcatccatc ttttgagcgt
    30781 agacagcaga agagacacct agacgtgctg cattatcttc aatgagtttg actttgtgtt
    30841 cgtgtacatc taaagcgacc acttctccac cgcatgacgg atctaaaaaa gaagcaatat
    30901 gcgtcgtctt acctccgggt gctgcacagg catctaagac atgatgttca ggctcgattt
    30961 gcatagacgg cgctacaagc atcgaacttt catcttggat cgtcatgacg ccatcgtgga
    31021 acaatgagct agaagctaaa tgcccttttt tcgctactat accgtatgga ctgacttggc
    31081 ttttttcagc tgtgatctct tcttcttgca aaacagtgat ggcctgatct ctggaaagtc
    31141 ggcgtgtatc tactcgggca ctagcatggc taggctgata tagcgataaa ccgagctttt
    31201 cagtttcttc gtatccgatt tgatccacca gtcgtttcgt catccataga ggcaggctga
    31261 ttttgacaga aagtctttcg ataggatcag caatgccttg cagatctggt gctccttgac
    31321 gctgataatt cctcaagaca ccatttacaa atttccctgt cccaggattt ccttttactt
    31381 ttgcgatttc gaccgcctcg tttaaaatag catgtgcagg gactttatct aaaaattcta
    31441 attggtatac agaaagataa agaagcatct tgacccagtc atccactttt tttgctttag
    31501 cgatcaatgg tgcaacgtaa aattccaata gtcgctgatg gctaatcgta ccatagatca
    31561 attctgtaaa taaacccaca tctttttgat ttagctcgct ttgtttgatc atctcattca
    31621 acaaaagatt cgagtaggcg ccacccttgt tcactcgctc taatgtcagt aatgccatat
    31681 atcgtactga ttttttcatt tttttaggga tttttttcat tatttcacct gctgaccaat
    31741 ctgaacttgc tgaccgcttc cgttaaggaa atcatgtacg gcctgttttc ctttaccagc
    31801 tggctgtaat tcattgatgg ctaagacggt ctgtttaccg caagcaatcc atagattttt
    31861 tttgtctttc cggatgatcg taccaggtgc tttgtctgta gtttctgcca atacttcaac
    31921 cgataataat ttccagcgta tgccctcata ggtcgtgaat gcaattggcc aaggacgcat
    31981 tccgcgtact tggttgtcaa tttcttctgc agttttatgc caatcgattg cttcttgttc
    32041 ccgtgtgatg tttggcgaaa aagttgcttc tgactcgtct tgaggtactg gtttttgtcc
    32101 atccaaaata ttgggcaatg tttctagcaa tagctttctt cctaaaaggc taagcttttc
    32161 aaacatcgtc cctacatcat cttgtttagt gattggtatg ctttcttgcg catagattcc
    32221 gcctgcatcc atttttttga tcatttccat gatcgttaca ccagtttctt tctccccatt
    32281 catgatggca taatgaacag gcgcacctcc ccgatattta ggaagtaaag aagcatggac
    32341 attgattgcc cctaattttg gtacttgcaa taacttttca ggcaagaatt ggccaaatgc
    32401 cgcagtgacg atcacatcag gcaccaattc aatgatttct tccatttctg gcgaaccgga
    32461 gattttctct ggctgcagca cacggatacc gtgcttcaga gccgcttttt ttaccggtgt
    32521 cggcgtaatt actcgtttcc gcccaactgg acggtcgggc tgtgttacca ctgcctggat
    32581 ctcgtaacct gattctatca gcccttcaag aatcggcaca gaaaaagcag gtgtgcccat
    32641 aaatactagc ttagtcatca agatgttcct ccatatattc ttcaagttct tcttccggga
    32701 tctgctgtat cattttctca atgaacaaga taccgtctaa gtgatcgatt tcatgctgga
    32761 aggcacgggc taaatagcca aaagccgtca cttctatctc ttctccgtct cgatcataat
    32821 aacgcacggt cacttcatct gctcgcttga ccgttccata gacatgcgga atactgagac
    32881 aaccttctac gtcaaggctt tcaccttttg cttcgatgat ttctgggtta atcagttcaa
    32941 atttctcgcc ttcatctact tcgatcacag caattctctt gttttgtcct acttgaggag
    33001 cagcgatccc tattccgtca ttcgcaatca ttgtctcata taaattgtct aataaagcga
    33061 ttgtctcgtc cgtgattaca tcgattggct gagcagttct ttttaattta tcattagggt
    33121 ggatcaatat aggatagcgc ataatttggc cctttcttaa ataaagttta atggttcgtt
    33181 gtcaattgcc acaaaaagac cttgtcgctg ttctttctgg cttccattca aaatttcatc
    33241 caaaacatga ggaagattcg gttcatgttt atatttgatg attagttgat aataatatcg
    33301 gtttttcact ctcaaaatca tgctgggcgt tggccctaat agcaagcttt gagggcttaa
    33361 cacttctttt agctgattgg caatctgaaa gattttcttt gccgctactt gttcttcagg
    33421 atggctggcg gttattttca cagtaaagta ataaggagga tagcctccac gatgccgtac
    33481 atgcatttcc tgctgataaa actgctcata attttgtttt ttagccaatt ctatggcgta
    33541 atgttgagga ttgaatgttt ggatcacgac ttcgcctgct ttttccgctc gccctgctcg
    33601 tcctgaaact tgggtaagca attggaatgt atgctcacta gaacgaaaat ccggtaaatt
    33661 cagggaggta tcagcattca acaccccaac taatgtcact tctggaaagt ccaaaccttt
    33721 agcaatcatt tgtgtcccta ataatatatc tgcttctttt gcaccgaatt tttgtaaaat
    33781 ctgttcgtgt gccccttttc ttcgagtggt atctacatcc atacgcaaaa tcctagcttg
    33841 cggataaagt tctcggagtt cctcttctac tttttgagta cctgttccat agtagcggat
    33901 tttattgccg ccacaattag gacagcgatt gggaataggt tcttcatgtc cgcaataatg
    33961 gcagcgcatt gaacgtgtat ccatatgcag tgtaagagag atatcgcaat taggacatgg
    34021 aagaacaaat ccacagtccc tacacatcac aaaagacgaa tatcctcgac gattgagcag
    34081 taggacagtt tgttcttttt tgtctaatcg attttgtatt ttttcctgca aattagcaga
    34141 aaaagtagag gtccgatgat tctgaaactc ttcacgcata tctacgatct caatagctgg
    34201 aagctgtgca gctgctttgg ctcggtggtt taactgcaat aactgataaa cgcctttttg
    34261 agctcgtgct cttgattcta aagagggcgt cgcgcttcct agaactattg ggcaatgatg
    34321 atatttactg cgccaaattg ctagatctct cgcatgataa cgcggtgtct catcttgttt
    34381 gtaacttgat tcatgttctt catcgataat gatcagcccg atatttttca gcggggcaaa
    34441 aatagcagat cgtgcgccta ctactacttg cgcttcttcc cgctcaatct tgcgccattc
    34501 atcatatttt tcaccttggg aaagtccgct atgtaagacc gcaactgatt ctccgaagcg
    34561 tcctttgaat cgctcgacca tttgcggagt caatgcgatt tcaggcacta acataatagc
    34621 cgttttattt tctgacaaaa catcagcgat tgcctgaaga tatacttctg ttttcccgct
    34681 gccagtgatt ccctccagca aaaagacctg atctttttgc tgctgacctg ctgtgatgat
    34741 ttgttcgaca gcattttttt gttctgcatt cagttccaat gccgtcgttt ggtcaaatac
    34801 tcggtcttta taagggtcgc gataacgttc tgattctata aactccagcc agcccttttc
    34861 ttttccttga ttcaagacag cagtactgat tcctttattt ttgaaataag caattggttc
    34921 ttcttggctc agttcagata aacacaaaag aagttctttt tgtttcttgg cattttgccg
    34981 taattttgcc cattcttctt cgatttgctt ttcagttaac aaagaacgga ccaaacgaat
    35041 cgtttttact ttatttcgtg tatggacttc atatttgata tctactaatt gctcttggcg
    35101 ccagcgaatc aattgagaaa ggctgccttc ttcttctgct tccttccagg aaatcacttc
    35161 tcgctgatca aacaatacct gaacttctgg tgtgtctgcc aaaggataga tcaatttatc
    35221 atattctgct ttcatcacac tcggaagcat cgtttgtaga caagtgattt tgaatgcgta
    35281 agtaattttt ttcatataat cagctaatga aagtaattct tcattgacca ccggagcaag
    35341 gtctagcaaa cgaataactg ccttcaaaga tggattagct gattcttctg atttttggat
    35401 agccataaca aatccttgga catgacgatt gccattacca aaagggactt ctactcgcat
    35461 cccgacttgc aaagctgttt ccatttcaga gggaaccaaa taagtgaatg gctgatcggt
    35521 ctgcatagta ggtacgtcca caatgacttc agctgttaat gtcatggttc gtttcctcct
    35581 ttttttgtct tttcttatca agcgacaaaa agttccttgc ctattttact agagatcgat
    35641 ggatttgtct tatcttctgc caaatcctaa caaaaaatta acagcagata agaaaaaaaa
    35701 gaactgtgtc aaaaagttca gacacagcct tttctaccag acaataagtg gcgaatgttt
    35761 gaaaaggaaa cagctgggtt tgcttttctt tttagatgtt cgctctattt ttctttatta
    35821 cattttttgc tcttcacgga tacgtgattc caattcgtgt tgttcacgct ctttttgtgc
    35881 ttgacgttcc tcttcttcca tcttcaaacg ttcacgtttt aattcaggat gtggatcatt
    35941 gactacattt cctgcatcga tttcttctaa agcttgaccg acgcttttta ctgaatcaaa
    36001 agaatctaga gtaggctgtg cacttgcatc caattcatgc gcgcgtttac ttgctaaaat
    36061 cactagagaa tattttgaat ttacacgatc taatagtgag tcaattgaag gttttaacat
    36121 cataagacta catctccttt aacattttaa tatatttacc gattacgcga tctactcgaa
    36181 aatgttcact ggcaataatg tttttgattc gttcaactgc tagcggcact tcatcattta
    36241 caacagcata gtcgtaaaga gccatcattt cgatttcttc ttttgcaact ttcattcgtt
    36301 tttcgattac ttcatctgca tctgttcctc ggcctacgat ccgtgatttt aattccgcta
    36361 aatctggcgg agtcaaaaag atgaatacac catcgggcac tttttctttg acttgttttg
    36421 ctccttggac ttcaatctcc aagaaaacat cttttccttc atccaatgtc ttgttgacat
    36481 atgacaaagg agttccataa taattcccga catattctgc gtattccagc atctctccag
    36541 cttcgatcat tgattcaaac tcttctttgc ttcggaagaa ataatcaacg ccttcgacct
    36601 ccccttcacg cattttacgg gtagtcattg agatcgaata ctgaaaatca ttatcctcac
    36661 tctcaaaaat tgctcttcgt actgtgcctt ttcctacacc ggatggacca gacaacacga
    36721 ttaataatcc acgctctgac atgacgacgt cctttcaact agtctttttg tacttatcgt
    36781 ttattttgca ctttttttct gcaactttca aggttatctt taattatagc tagaaattgg
    36841 taaaaaagct accctgtacc aagaggtcgt aaaaaagctg tcctgcatca agtaataaga
    36901 acagccaaac ataaacagct tctcatgttt ttcgtttggt tggggttatt atcgcagatg
    36961 caagcttttg aacgccgtgt ataagaggtc gtgaagaagc cgattagttt cgagtatgaa
    37021 gaaaaatttt gtgaaaatag tttctcacat ttttgcataa atttggctta ataccgagaa
    37081 aatggttttt gaacatcact tattcaaaat aaaaaaagat tgtgacacga attttgtcac
    37141 aacctttaaa tgtattccta gagcaaagaa gctaagcgaa gaaatccaat tctgtttatt
    37201 tattgtattt ctttgttcag catccttgct aaaagcccat attggacaaa gtattcacta
    37261 cacccatgat ctcatctttt cgagaacgtg ctttttctgt cggatcttct acctcttctg
    37321 tgtcaattcg aacaataaat ttccgattca tcgtcacgta agcaccgtcg ttgatcaaaa
    37381 taaacagttc gttctcatta tagtctgcac aggcatcttt ccatacgtca aagccgtttt
    37441 tttcagaagg gatatcccgt ttaaccacag tttttccact taattcgtgg aacgttacgt
    37501 cataatattt cattatctct acctctttcg tcattcaata ttttgtactt gttctctgat
    37561 tttttctaag atcgtcttag actggacgac ttgattttta atttcgattg catttgattt
    37621 ggaaccgatc gtgttgattt cccgattcat ttcttggatc aggaaatcta attcccgacc
    37681 tacaggacat tgtgcttcga taagttcatc tagtttttgt acatggatcg ccagacgatc
    37741 cagttcctcg tgaatatctc cgcgttccaa caagatggct agctccgtca acaaccgttg
    37801 ctcatcgact ttcgctccaa gaaattcctc cagctttttt tgatacctta tttgaaattc
    37861 cttttcatag atttctacaa aactatcgag tttttttagt acttctttga attcttcagt
    37921 attctttttc aacactaaag ctaaagcttg accttctttt tctcgactcg cctcaataga
    37981 atcaagtgcg cttttcaatg tatctagagc taactgtgcc aattcttcca tgctctcttc
    38041 cggtttttct tctaccgtta cgaagttttc gtccagcacg aagcgttccg ccatccgact
    38101 aattgacact tgattttgac taccgtatcg ttgttgaatt ccgtctgtca attgagtgat
    38161 aagtgaatca atcaattccc agttgatgac gatctgtttt tcgtttttgc caatataagc
    38221 tagattcaca aatacttcga ttcgaccgcg gcttaactgc tgtttaataa gctgacgaaa
    38281 atcgttttca acaaagttca ggattttagg acttcgtatt tgaacatcta gaaaacgctg
    38341 gttgacactt tttatttcaa tatctaactg atacgctttt gtttcgcgga tggctttgcc
    38401 aaaaccagtc atacttttca ttcgacaggg tccttcctca ttacatattt tctattgctt
    38461 tttgcagttg ttcaaactct tcgttcgtca atgtcaggcc tttccccatt ttttcatggt
    38521 ctggcgccca atctcgaaga tcgaattttg gcgggcgacc attccagctg actaggttca
    38581 gttctttacg ccaccccttg ttgttttctg acaatatagc gatttcttct acgatttcat
    38641 atgaaaattc ttgtgccatg gttgttcctc cttgagataa tataaatata ggatcagtaa
    38701 tgtgtcaact ggttgcttaa gcaattcatg ctgcaaacga tccttgcgtt ttccttgtag
    38761 cagataatca aaataaacag ttttcgtgcg ctcattttgt attttttgct ggtattttcg
    38821 ataccttcgc cattcttcag tacttaattg accaatttgt gccaataaat catccggcat
    38881 actttttata tcttgttggc agatcatttg aatcatcatg tctacttgat agatttcatt
    38941 gggcagtaaa aaatgttttt ttgctaaaag ttctagcatc gccaatccgc atgtctctgg
    39001 tacgaccctg ttcttatata aattacgcag tttttgccaa aattttatgc ttttgaaaaa
    39061 agataattct aactgaattt cttttgttaa gtaagacaaa aatcctcgcc agtatgttct
    39121 tgcaggttta acagaatgaa atgtatacca gttcccatag taatagccta atcgtttttt
    39181 catttccaga tagcctaatt gtaaatttaa ttggttcctt tggctatcaa aaagcagaaa
    39241 gctgcctagt gtccacaatg tttgacattt ccaatggata aaaatgtctg gcatacgaat
    39301 ttttttcgct gggcctggcc cttgcacatc tacgacaaat gcctctgttg ctcctttgtt
    39361 aatcgcaata tcaataggta ttttgttgcg atacccaccg tctgcatatt tattttttcc
    39421 gatctttcga taagccatag cgggataaaa tgaagcagag gcgagtatcc aatctggtat
    39481 ttcttctgtt tttgtctgtt ggatatcagt cactacttcc gctagttctg gcagtttagt
    39541 agacaccgta tataaacgta ttttgggatt atgtttgagt aaggacaaat ccaaattgtc
    39601 tttgaccatc ttttccaatg gagcagaaga aaccccgcct tcaattagtg ctgttttcgt
    39661 catttggcgt ctttctcgat agaagcgttc acgaaggtct tcaactgcag ccatttctgg
    39721 taacgcaagc acttgtcttg tcgacagctt tttccataaa ttcacagctt ttttcatatc
    39781 tccttgtaaa atcaatgcgc cgttcaacgc acctaccgag gtacctgtga taatctcaaa
    39841 gttgatatca tgttccttca gcgcctgcca cacaccaatc tgataagcac cgtgcgctcc
    39901 tcctccacct aaaacaagtg ctgtatgata gaccagcttt ttatttaatt gtgtggttcc
    39961 ttgatagccg tttttatgaa aaatacgcaa ccattcttct gatagaggag attgaaataa
    40021 aaaacaacag ttttctttaa taaaaaaacg agcgcaagct tccatatacc gaaaaaattt
    40081 tttccattca aaagtttcac cttcaatcaa cagattatta aaaataacat ctcctctact
    40141 tttagcaata gtaaaataga cggtatattt tttcgatact gctttaaaag cacattgctt
    40201 ttttgcagaa cgccaaaaag cgtctctact ttcttgatga ctttgaaaaa atggaagggg
    40261 ttgttttgct tgctggatag gcgaatacag cgacattttc tgtgaaaagt aaactttttt
    40321 tatctccatc gcgctctcct ttttcttcaa gtataccagt acgtcataaa aaggtcatga
    40381 gtctcgtatt cgactttgta gattccataa gaaaacaatt cataaaacct cctttaaata
    40441 aaaaaataca aaaatcacaa aaattaattg aaaatgagag tgttttcaat gaaaacgtga
    40501 ttttgtaatg gaattttaaa ataaaagaaa caagcttttt taatgcaaat gttgtaaaat
    40561 aaaagagatt aagaggaaat aagaggagga acatgatgac tgagtcagtt tatgtacata
    40621 ttgataccac aagtaatgct gttttaacaa aagggctgac tgctactgac tttgcaaaca
    40681 gtatcgtgca ttttcctcaa aacttgctct tgcttgatcc atctgcttct gctggagaat
    40741 atgaatccca tacagggctg aaagtaatcc gtggtacaga aaatattcag cgtttctttt
    40801 cctcttctcg taacaggcgt ggattcgatg aattgaagtg gatcgacttt actgatttaa
    40861 cgatgctgaa agaattgaca cctttagaaa tttcagaatt attatatttt ggtcatatga
    40921 aaacacatct tcattcgccg tttttttata aactacagaa taattttgta tacttcgatc
    40981 ttaatgatca gctgaatcgg atttattatc gctatttaga tgaattttat aggatttttg
    41041 ctagtaaatt aagttcgatc ttgtcagata agatcaatgt aaaaaaatct tttttcaaaa
    41101 aagaaacacc tgttgaaaaa atcagtgtgg aattattgaa agaaatgcgt aatttgatgc
    41161 aggaagggat cattttttct tttgatcaag ttggtttgat cgatggtaaa tatcgtatcc
    41221 caatctatgt gattgaagat tatatttgga aattaaaagg cacccggtat cgagaagagg
    41281 aaatcatcgc tactttaatc tactctgtca ccgatc
//
LOCUS       DACDUU010000016        40583 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.016, whole genome shotgun sequence.
ACCESSION   DACDUU010000016 DACDUU010000000
VERSION     DACDUU010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.016"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            666..2276
                     /locus_tag="IWL30_000895"
     CDS             666..2276
                     /locus_tag="IWL30_000895"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAP9705681.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            2568..3437
                     /locus_tag="IWL30_000896"
     CDS             2568..3437
                     /locus_tag="IWL30_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAP9705682.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            3602..4873
                     /locus_tag="IWL30_000897"
     CDS             3602..4873
                     /locus_tag="IWL30_000897"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP9705683.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            4870..6165
                     /locus_tag="IWL30_000898"
     CDS             4870..6165
                     /locus_tag="IWL30_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAP9705684.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            6264..6527
                     /locus_tag="IWL30_000899"
     CDS             6264..6527
                     /locus_tag="IWL30_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAP9705685.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            6972..7421
                     /locus_tag="IWL30_000900"
     CDS             6972..7421
                     /locus_tag="IWL30_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9705686.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            7478..8896
                     /locus_tag="IWL30_000901"
     CDS             7478..8896
                     /locus_tag="IWL30_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9705687.1"
                     /translation="MKIVGVTACPTGIAHTYMSVEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            8896..9756
                     /locus_tag="IWL30_000902"
     CDS             8896..9756
                     /locus_tag="IWL30_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAP9705688.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            9930..10703
                     /locus_tag="IWL30_000903"
     CDS             9930..10703
                     /locus_tag="IWL30_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9705689.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            10850..11215
                     /locus_tag="IWL30_000904"
     CDS             10850..11215
                     /locus_tag="IWL30_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAP9705690.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(11363..12571)
                     /locus_tag="IWL30_000905"
     CDS             complement(11363..12571)
                     /locus_tag="IWL30_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9705691.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(12590..12868)
                     /locus_tag="IWL30_000906"
     CDS             complement(12590..12868)
                     /locus_tag="IWL30_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705692.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            13061..13717
                     /locus_tag="IWL30_000907"
     CDS             13061..13717
                     /locus_tag="IWL30_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP9705693.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(14159..14956)
                     /locus_tag="IWL30_000908"
     CDS             complement(14159..14956)
                     /locus_tag="IWL30_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAP9705694.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(14953..16137)
                     /locus_tag="IWL30_000909"
     CDS             complement(14953..16137)
                     /locus_tag="IWL30_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP9705695.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(16162..17409)
                     /locus_tag="IWL30_000910"
     CDS             complement(16162..17409)
                     /locus_tag="IWL30_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAP9705696.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            17624..19369
                     /gene="dnaX"
                     /locus_tag="IWL30_000911"
     CDS             17624..19369
                     /gene="dnaX"
                     /locus_tag="IWL30_000911"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAP9705697.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            19389..19703
                     /locus_tag="IWL30_000912"
     CDS             19389..19703
                     /locus_tag="IWL30_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAP9705698.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            19728..20324
                     /gene="recR"
                     /locus_tag="IWL30_000913"
     CDS             19728..20324
                     /gene="recR"
                     /locus_tag="IWL30_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAP9705699.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            20388..21032
                     /locus_tag="IWL30_000914"
     CDS             20388..21032
                     /locus_tag="IWL30_000914"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAP9705700.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            21047..21376
                     /locus_tag="IWL30_000915"
     CDS             21047..21376
                     /locus_tag="IWL30_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705701.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            21390..22328
                     /gene="holB"
                     /locus_tag="IWL30_000916"
     CDS             21390..22328
                     /gene="holB"
                     /locus_tag="IWL30_000916"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAP9705702.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            22364..23188
                     /locus_tag="IWL30_000917"
     CDS             22364..23188
                     /locus_tag="IWL30_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAP9705703.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            23181..23528
                     /locus_tag="IWL30_000918"
     CDS             23181..23528
                     /locus_tag="IWL30_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAP9705704.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            23597..24469
                     /gene="rsmI"
                     /locus_tag="IWL30_000919"
     CDS             23597..24469
                     /gene="rsmI"
                     /locus_tag="IWL30_000919"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAP9705705.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            24578..25699
                     /gene="dinB"
                     /locus_tag="IWL30_000920"
     CDS             24578..25699
                     /gene="dinB"
                     /locus_tag="IWL30_000920"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAP9705706.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(25753..26355)
                     /gene="nrdG"
                     /locus_tag="IWL30_000921"
     CDS             complement(25753..26355)
                     /gene="nrdG"
                     /locus_tag="IWL30_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAP9705707.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(26670..28859)
                     /gene="nrdD"
                     /locus_tag="IWL30_000922"
     CDS             complement(26670..28859)
                     /gene="nrdD"
                     /locus_tag="IWL30_000922"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAP9705708.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            29185..29952
                     /locus_tag="IWL30_000923"
     CDS             29185..29952
                     /locus_tag="IWL30_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9705709.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            29942..31984
                     /locus_tag="IWL30_000924"
     CDS             29942..31984
                     /locus_tag="IWL30_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9705710.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            32265..32414
                     /locus_tag="IWL30_000925"
     CDS             32265..32414
                     /locus_tag="IWL30_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAP9705711.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            32425..33939
                     /gene="dltA"
                     /locus_tag="IWL30_000926"
     CDS             32425..33939
                     /gene="dltA"
                     /locus_tag="IWL30_000926"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAP9705712.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            33936..35141
                     /gene="dltB"
                     /locus_tag="IWL30_000927"
     CDS             33936..35141
                     /gene="dltB"
                     /locus_tag="IWL30_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAP9705713.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            35186..35419
                     /gene="dltC"
                     /locus_tag="IWL30_000928"
     CDS             35186..35419
                     /gene="dltC"
                     /locus_tag="IWL30_000928"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAP9705714.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            35422..36690
                     /gene="dltD"
                     /locus_tag="IWL30_000929"
     CDS             35422..36690
                     /gene="dltD"
                     /locus_tag="IWL30_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAP9705715.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            36831..37913
                     /locus_tag="IWL30_000930"
     CDS             36831..37913
                     /locus_tag="IWL30_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAP9705716.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            37913..38716
                     /locus_tag="IWL30_000931"
     CDS             37913..38716
                     /locus_tag="IWL30_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP9705717.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            38786..39526
                     /locus_tag="IWL30_000932"
     CDS             38786..39526
                     /locus_tag="IWL30_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9705718.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            39537..40541
                     /locus_tag="IWL30_000933"
     CDS             39537..40541
                     /locus_tag="IWL30_000933"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAP9705719.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
BASE COUNT        13042 a         7107 c         8458 g        11976 t
ORIGIN      
        1 ttttgtcgca tctccttatt ttttacttat gtgattcgtt accttcaatg aatccctaca
       61 aggcactttt gttgaaaaaa aggtcatctt tgaaaaaatt agtggaattg ttttgttttt
      121 gatattattt aatagaaaag atatgccaaa aatccgagaa ctgtggacta cgttttttac
      181 gtaaaggagg taaataaggg tgaaagaaaa agattgtttc tacgataagc tatataaact
      241 gttgctgcaa gtaggaaaaa gtagttcttt gttaggtggg atattatcta tcataatgtt
      301 tgttttattt ttttatctat tatttcactt aaaatttttc agtaacgatt ttctgatttg
      361 cttaggttta acgttagcta tgtttatatc aagcttatta tgtgggttaa attttttttg
      421 cactcataaa ggacttaaga aataatttct caaaggatat cgaaaaagaa aaaattgtaa
      481 gcggatagta ttcatttgag tacttgactt tcccttagtc agcttgtatt atacaccttg
      541 ggcacccttt tagagggtca gaagtttaca gaatttcggc tccctattct tattgaagaa
      601 tagggagctt tgtttattgt ctgagacttt tcctgcacat aaattaaaaa ggagtttttg
      661 aacttatgac aaaatatatt tttgtgaccg gcggtgtcgt ttcatcaatc ggaaaaggga
      721 ttgttgcagc ttctttagga cgcttattga aaaaccgcgg cttaaaagta actatccaaa
      781 aatttgatcc atacatcaac gtggatccgg ggacgatgag cccatatcag cacggagaag
      841 tattcgtaac agatgatggt gctgaaacag atttggatct gggccactac gaacgtttta
      901 ttgacatcaa tttgaacaaa tactccaacg tgacgacagg aaaaatctat tctgaagttt
      961 tgcgtaaaga acgtaagggc gaatacttgg gagcaactgt tcaagttatt cctcatatta
     1021 caaatgagat caaagaaaaa atcatgcgag cagctaaaat gactgattca gatgtgatca
     1081 ttacagaagt agggggaaca gtaggggata tagaatcttt gccattcctt gaagcacttc
     1141 gtcaaatgaa agcagatgtc ggtacagata atgttatgta tatccataca acattgattc
     1201 cttacttgaa agctgctgga gaaatgaaaa ctaaaccaac gcagcacagt gtaaaagagc
     1261 tacgtggttt aggtatccaa ccgaatattt tggtggtacg tacagagcaa cctgtttctc
     1321 aaagcgtgaa aaacaaattg gcacaatttt gtgatgtcga accagaagca gttatcgagt
     1381 cgccagatgt agatacactt tactccatcc cattagtttt acaagcacaa ggaatggatc
     1441 aaattgtttg cgaccattta aaactagata caccagaagc agacatgaca gaatggaaag
     1501 aattagaaga acgtgtcttg aacttgaaga aaaaagtacg gattgcgctt gtcggtaaat
     1561 atgtcgagtt accagatgca tatatctcag tagttgaagc attgaaacat tctggtttcg
     1621 cctataattc agacatcgag atcgattgga tcaaagcaca agaattaacg agagaaaatg
     1681 tagaagaacg tttgaaagac gcagatggaa tcttggttcc tggtggattt ggtgatcgcg
     1741 gagtagaagg gaaaatcgaa gcgattcgtt atgctcgcga aaatgacgtt ccgtttttag
     1801 gtatttgtct tggtatgcag atggcttgtg tcgaatttgc acgtaacgtc gttggattag
     1861 aagatgcgtc atcagctgaa acgaatccag atacagcaaa caatattatt gatttaatgg
     1921 ctgatcagga aaacatcgag aatttgggtg gaactttacg ccttggatta tacccatgta
     1981 aaatcaaaaa aggaacgaag acggctgaag catatgatgg agccgatgtt gtgcaagaac
     2041 gtcaccgtca ccgctatgag ttcaataaca aatatcgtca attgtttgaa gaacaagggc
     2101 tagtcttttc aggtgtctca ccagataacc gtttagtaga aatcgtagag ctgccagaga
     2161 aaaaattctt tgtgggatgc caattccacc cagaattgat ttctcgtcca aatcgcccgc
     2221 aaaaattaat caaagcattt gtcggagcat cattagcaga tcacgaaaca aaataatttt
     2281 tgaacttgat agaaagagaa aagtagcaag agaaggggat aagcaatttt cttgtcactt
     2341 ttctcttttt ctgatttgtt attatttgta ggaaaaaaga tagaaagcaa aagacttttt
     2401 tcgattaaag cgctattgga tattttattt tttgcaaaaa acttcttgaa aattttcatt
     2461 ttcattgatt ttagtagaaa actaccgaaa tgtttggtaa aatagggtcg ttggttaaga
     2521 attgtcttaa caaatttagt tttacaaaac ttaggaggaa ttttattatg ccagtagtat
     2581 caggagctga atttttaaaa gctgctcgta aaggcggtta cgctgtcggc ggattcaaca
     2641 caaacaactt ggaatggact caagcaatcc ttgaagcggc tgaagcaaaa caagcaccag
     2701 tacttatcca aacatcaatg ggtgcagcta aatacatggg tggttacaaa atctgtaaag
     2761 atttaatcac tgatttagtt gaagcaatga acatcactgt tccagttgct atccacttag
     2821 accatggtga ttacgaagct gctttggaat gtatcgaatt aggctatact tcagtaatgt
     2881 tcgatggttc acatctacca tttgaagaaa acttgaaatt agctaaagac gttgttgaaa
     2941 aagcacatgc aaaaggtatc tcagttgaat gtgaagttgg ttcaatcggc ggagaagaag
     3001 acggaatcat cggtaacggc gaattagctg atattgaaga atgcaaacaa atggttgcta
     3061 caggtatcga ctacttagct tgcggtatcg gtaacatcca tggtcaatat ccagaaaact
     3121 ggaaaggatt agcttttgac cacttacaag caattgctga tgctgtaggt ccagacatgc
     3181 ctttagtatt acatggcgga tcaggtattc ctcaagatca aatcgaaaaa gctatctcaa
     3241 tgggtatctc aaaagtaaat gttaacactg aattccaatt atcatttgct gcagctactc
     3301 gtaaatatat cgaagctggt aaagataaag aaggtaaagg attcgaccct cgtaaattat
     3361 tagctccagg taaagcagca atcgttgaag atgctaaatc tcatatcgac tggttcggtt
     3421 cagctaacaa agcgtaagca tccacataac agatacgata tataggaatg gagaacgact
     3481 tttgaagtcg ctctccattc ttttttactt aaaacggcag accatgctcg tttctttttt
     3541 ttatctatga taaaatacaa gcatatccat gaaaaaagat taataaacaa ggtggaaaca
     3601 gatgaaaaaa atcgtaatca aaggaaaccg acctttagct ggtgaagtaa cgatcagcgg
     3661 tgcaaaaaat agtgcagtcg cattgattcc agctgcaatc ttagctgatt ccccagtgat
     3721 cttggaaggt gtgccggaca tacaagatgt tcattcatta attgaaattt tagagatcat
     3781 gggcgcaact gtccattttt ccaacaacat actagaaatc gatcctcgag aagtcgtatc
     3841 gattccgatg ccgcacggaa aaatcaatag tttgcgtgct tcttattatt ttatgggaac
     3901 actgcttggt aaatttggcg aagcggtagt aggcctgcca ggtggctgtt atttaggccc
     3961 tagaccgatt gacctacacg tcaaaggatt tgaagctcta ggtgctgaag tgacaaatga
     4021 acatggtgcc atgtatttac ggacagaaaa taaaggattg cgtggcaatc ggattttcat
     4081 ggacgtggtt tcagtcggag caacgatcaa tgtgatgtta gctgctgtaa aagcaaaagg
     4141 acagacagta attgaaaacg ctgcacgaga accggaaatc atagatgtgg caactttact
     4201 gaataatatg ggggcaaaag ttcgaggagc gggtacagat gtgatccgta tcgaaggagt
     4261 cgaaacatta catggctgtc ggcatttcat gattcctgac cgcatcgaag caggaaccta
     4321 tctagcatta gcagctgcag taggaaatgg tatcaaagtg aaaaatgtga tctttgaaca
     4381 tttggaaagc tttatagcaa aattgcagga aattggtgtc catatgaaaa tcagcgaaga
     4441 tgaaatcgaa gtttacccct cgaaagaatt aaaacccgca aacatcatga cctatcctta
     4501 tccaggattt gccacagatc tgcagcagcc acttactgcg ttactattga tgactacagg
     4561 cacttcagaa atcatcgata cgatctatgc gaaacgtgtg aaccatgtgc cggaactagc
     4621 acgaatgggc gctgatatta ccgtggaagg aaatatgatc atcgtgaatg gtccaaataa
     4681 acttcacggc acagaagtcg tggcttctga tcttcgggca ggtgcttgtt tagtgattgc
     4741 tggactattg gcagaaggga caacgacgat ttacaatgta gattatatct tacgcggtta
     4801 tgatcatatt atcgagaaac taacagcctt aggggcatca attgaaatga tagaggaagt
     4861 gggagctgaa tgagcgatta tttgacaatg gcagaactag agaacaaaac gttaaaagaa
     4921 gtttatagtt atgccaaaga atttaaaatt ccttattaca gtaagatgaa taaaaaggaa
     4981 ttatctctag ctgtgattcg tgcacaagca gagaaacaag gattttatta tatggaagga
     5041 atattggata tcgttggtca agatggttac ggttttttac ggccaatcaa ttatggacca
     5101 agtgcagaag atatctatat ttctgcctca caaatcagac gattcagttt gagaaatggg
     5161 gacaaagtgg caggaaaagc ccgtccacct aaagaatcgg aacgttatta tggtttgatg
     5221 cacgtggaaa gcgttaatgg aaaagatccg gaagaagcaa aagaacgtcc gcattttcca
     5281 gccttaacac ctctttatcc gcaaagacag cttcaactgg aaacaacacc gcaaagacta
     5341 tctacccgaa tgatcgatat cttttctcca atcggttttg gtcagcgtgg attgatcgtc
     5401 gcaccgccaa aagcagggaa aacttctgta ttaaaagaaa tcgcaaatgg tatcaccgaa
     5461 aactatccgg aagtagaatt aatcgttcta ctgattgatg aacgaccgga agaagtaacg
     5521 gatctagaac gaagcgtgaa aggcgacgtg gtatcttcta catttgatct gcagccgcaa
     5581 aaccatactc gagtttctga attagtattg gaacgcgcaa tgcgtttagt cgaagataaa
     5641 cgagatgtcg ttatcttgat ggatagtatt actagacttg ctcgggccta taacttagtc
     5701 gttccaccaa gcggacgtac attgagtggc gggattgacc cagccgcatt gtataaacct
     5761 aagaaatttt tcggggcagc aagaaacatc gaagaaggtg gcagtttgac gattttagcg
     5821 accgctttag tcgatacagg aagccgtatg gatgacgtga tctatgaaga attcaaagga
     5881 actggaaatc tagaattaca actttcacgt gaattatctg aacgtcggat cttcccagcc
     5941 atcgatatca aaaaatcagg tacccgaaaa gaagaattat tgatgccaag tgaagaatta
     6001 gaagaaattt ggaaacttcg aaaaaatatg actggagatt cattagaata tacggaacaa
     6061 ctgatccgct ttttgcgtaa aacaaaaaac aatcagcaat ttttcaaaga atttcaagat
     6121 gtctcctttg gtaaaaaaaa tccgaccaga aatctgaaaa gataattgca accttgactg
     6181 aaacatgtta agatgttaat gttgtaaatc gacaaatcaa ctctggttca gcaaatggag
     6241 cagggcaaag gagcgaaaaa atcatgaaag aaaacatcca tccagattac cacccagtag
     6301 tattcatgga ttcaacaact ggtttcaaat tcttgtcagg ttcaacaaaa acttcacaag
     6361 aaacagttga atgggaagac ggtaatacat acccagtcat ccgtgtcgaa gtaacttctg
     6421 actcacatcc attctacact ggacgtcaaa aattcacaca agcagacgga cgtgtggacc
     6481 gtttcaacaa aaaatacggt ctcaaagacg caaacgctgc ggaataataa aaacgctgat
     6541 aactcaacgt ttagcagact atctgatagg gatagtctgt ttttttatgt tttttgccaa
     6601 tttaaaactt cgtttgatgc tgaaaaagtc gtaggatcat aatgcacaaa acaatgaaaa
     6661 actagtatat agttttaatt atccacttga taaaaacacc ttatattaac gaaatgaaga
     6721 ggggttattt cgaagtaaaa aaccatttac ctaaaattag aagctaagct ttcctttcaa
     6781 ggaaggcttt ttgttttgtt atagacttgc gattgtttgt gagctagatc gttcatttta
     6841 atgacttata cacgcggatg atgggtcttt ttcctctgtt cattcataat attgaacggt
     6901 acagctagcg atgctatact gacctcagtt ccaagaaaac gttaacattt tttgtggggg
     6961 agaggttttt tatgaggttt gatcaattga tcgcagaaga actgattgtt ttcgatccat
     7021 cgatagattc aaaaggatca ttatttgagc gaatagggga tgtgttgaaa aaaacaaata
     7081 aggtaagaaa tgttcaaaaa gtcgttaagg cactattcaa acgagaggag gagatctcca
     7141 caggaataga agatggattt gggattcccc atgcaaaaag caaatatgtg acaaaaccag
     7201 cactgtgttt ttttcataca gccaaattaa cggactatag cgggatagat gaaatgccaa
     7261 ttgaatatat ttttgccatc gtagttcctg aaaaatcagc agatatgcat ctagagattc
     7321 tcagcacact ctccagacga ttgatgaatg cagattttag aaagggaata aaagaagcca
     7381 ctacgccagc agatatcatc agacagatta gtcaagaatg aaaatagtag agtctttgtc
     7441 cgagtatcga tagtgaagaa aaaggaggaa aataatcatg aaaatcgttg gtgtaactgc
     7501 ttgcccgact ggaattgctc atacttatat gtcggtagaa aaattagaag taacagcaag
     7561 aaatctaaac catcaagcaa aattcgaaac acaaggtgta aaaacagaaa atcagttatc
     7621 tgaacaagaa atcaaagagg cagatgcaat cattcttgca gtagataaag aaatcgaatt
     7681 ggaccgattt gccgggaaaa aagtaaagcg tgtctctaca agccgagcaa tcaaagaacc
     7741 gcaagtagta atagacgaag cattgagaga tattggcgta ttcgttgtgt ctaatgaaaa
     7801 agaaccagct gcaaatgaga aaaagccgac aatctataat catttcatga atggtgtgaa
     7861 ttacatgctt ccatttgtga tcgcaggcgg aatcatcatc gcactgagtt ttgcctttgg
     7921 gattacagcg gctgatccca actcagcaga ctataatgtg ttagcagctg cattttcaag
     7981 gatcggagga gatacagcgt ttgccatgat ggttcctgcg ttaggcgcgg ggattgctac
     8041 atcgattgct ggaaaagcag gttttgcccc aggaattgtt gccggattac tggcctctac
     8101 aggtggttca ggttttcttg gcggaatgat tggtggcgtg ctagcagggt atatctgtga
     8161 ttttctggca aataaaatta gcgtaaaaaa agaattttct gctatttatc agctgattgt
     8221 cgtccccttt ttatcgatct taattattgg gcttttgatg gtatttgtga ttgaacaacc
     8281 gatcacatgg gtgttggatg ctttgacaaa ttggctgaat agcttgggaa atacatccgg
     8341 gctattgttc ggattactta tcgggatcat gatggcagct gatatgggtg ggccgatcaa
     8401 taaatcgact tcgactttct cgatcggttt gatgtcagct ggagtaaatg caccgattgc
     8461 tgcttgtatg gctgcaggaa tgacacctcc cctaggtatt gctttagcca ctttattatt
     8521 caaacataaa tttaccaaag aagaacggac ttcaggtcaa tcatgctgga ttttaggcgc
     8581 ttcgtatatt acagaaggtg ctataccgtt tgcggtatct gacccgatcc gcgtgatacc
     8641 tagtttgatg acaggttcag cagtcgctgc agggatttcc atggcagtag gagttacttc
     8701 aatggcacct catggaggga tttgggtgat gctaattcca aatgttatga acaatttacc
     8761 tatgtatgtc ttggcaattg catcaggtac agtcactaca gcctttatgc ttggactatt
     8821 gaaaaaaaca gtgaaagaga aaaaacaaga caagtggacg aaaaatgaaa atctaacaaa
     8881 tgaggtggaa gcgtaatgtt atatacaatg aaagagttat tgaaagtggc taaagagaat
     8941 caatttgcag taccagcctt caatatttgc agctatgata tgatgaaagc aatcatcgaa
     9001 gaagcagaga gattagaagc accagtaatc ttagaaattc atcctgatga aatcgcctat
     9061 ttaagtgatg agtttgttgc ggcagtaaga gcctatgctt atacaagtaa actgccgatc
     9121 gttatccact tggatcatgg cggtacgata gaagatgtct tacgggcgat tcgaaacggc
     9181 tattcttccg tgatgatcga tgcgtctttg aaatcattcg acgacaatgt acagattacg
     9241 aaaaaagtgg ttgcgttagc tcatgcggta aatgtctcag tggaggctga gcttggaacg
     9301 attgggaata atggttctgc tgaaggaggt gctgaaacaa tcatttatac agatcctgat
     9361 caagcgaaga tttttgtgaa agaaacagga atcgatacac ttgctgtagc aattggaaca
     9421 gcgcatggct tgtatccgaa aaacagaact ccaaaattaa atatcgactt actgaaagaa
     9481 ttgaaccaac aaatcgatct tccatttgtt ttacatggcg gttcaggtaa tccggacgca
     9541 gaagtcagcg aagcagtaaa atacggagtt gccaaagtga atttgagctc agatcttaaa
     9601 agcgtctttt tcaatgcttt gagagaaata ttgagtgaaa atccatcgat gtacgagccg
     9661 aatgcaattt atccagcagc taataaaaag gtacaagaag tggtcaaaca taaaatggaa
     9721 attttgaaca cagtaggcaa ggctagttta tactaaaaat aaaaatgtgt ggtcaagagg
     9781 gtatgacaat agtcttgtca taccctctta accacacacg atgttcagaa gtagtttttt
     9841 cgaaaaattt cttcttattt ctcgaagcca atcacttctg tcacaacctc ttttttcctg
     9901 ttactatata aagtaaatgg ggtgatttta tgttgaatga agtggagaat agacagcagg
     9961 agattgtgaa ttatctaaaa aacaaccaat ttgcaagagt caccgatttg atcgagttag
    10021 taaattatag cgaagccacg attaaacgtg accttgtttt tctggaaaaa agtggattga
    10081 tccgtagagt tcgcggtggc gccatgctta ttgacaatcg aaaaattgac gtgccttacc
    10141 tgatgaagat caccaaatta gaagaagaaa cagaaaaacg taagattgca gatacagctt
    10201 cttctttgat tcgagatgat atggtcttat tcttggattc cagtacaacc tctttacact
    10261 tagtcagaaa cctagcaaga tttgatggtt tgcagatcat tacgaatggc atcgtcacag
    10321 ctgctatgtt gagtgaatat acaacggcaa aagtatctgt attgggaggg gcaattgttc
    10381 cgaaacgagc aacaatcaac ggttcaaaag cctataacga tgcattgtca tattatgcgg
    10441 atttagcgat aatgagttgt cgcgggctag atttcagcca aggagtaaca gaaactcatg
    10501 aaggagaggc aatggtcaaa aaagctttta gaaaacaatc agaccggctt gccgttttag
    10561 ttacacagca aaaaattgca cataaattca tgcatcaagc cattgctttt catgaaatcg
    10621 attatctggt gacaagtaaa gaactgacaa aagaacagta tgcgaaagta aaggaacacc
    10681 atatcgaatg tctgtacgga taaattatta cgtgaatgtt ctaagtggct tcctcatttt
    10741 tagataggac cttatctttg ctcgcttctc tcagccgatg gtataataat gaaaaggtta
    10801 tcttgttaga taatcttact tttttatcca ggagaaagga gtgcagaaaa tgaaacatgt
    10861 gaaggcgttg gtagtgaagg caatcatgat ctgggcgatt ctgtgggtcg tgctgacagg
    10921 tttatacggt gttagcttta tggattcaac gattgttggg gtaattattg tagtcatgat
    10981 ctatgtgcta ggggatctgt tgatcttacg taaagtaggg aacatcgcag cgacgatcgc
    11041 agatgctggt tcagctgcag ttatcttgtg gctctatcta tattttatga atgatacagt
    11101 cgatatttgg atgaaagtat tgattcctgc tctattgatc ggtgtggcag aatggttctt
    11161 ccacaagtgg ttgctaagtc aaggcatcgt accagatgaa cggaaaatgg aataaatcaa
    11221 cagttaaact attcttatcg ataaatgaaa gctttaaaaa taaatagatg tttaattaag
    11281 aataatcata aactattttt actcaaaaaa ccgctcagat tattttcaga tctgaacggt
    11341 tttttttatc tattcatcaa gatcaaattt catttttcaa tgattcaatc aattcgccct
    11401 ctgcttcatg actatgtttt cggccattaa caagatagag tgctgtacat atcaatggaa
    11461 tgactccggc aatcacatac aatcctctaa atgaagtaag agattttagc atccccatca
    11521 aataaggacc aacgcctaag cctaggtcta aaccaataaa gtaggtagat aaagcaatac
    11581 tgattcgatg ttcgcggacg atttttaaac aaacagcctg tccatttgac ataaacgtac
    11641 cataacctag gccgactaat gctccagaga taagcaatgt cacactattc gtcgttacac
    11701 tcagtaaaaa taatcctgct gttaagaata aatagctagg atacatcacg tattgttctc
    11761 ctttgacatc gaaaatcctt ccagtcaatg gacgtgtaaa tgtgatgacc aaagcatata
    11821 cgacgaagaa aaatgaccca gcagcaacta agtggattac ttttgtataa gaagataaaa
    11881 atgataaaac acttgaataa gaaagaccca tcaaaaaggc aattcctgta atgaaaagtg
    11941 ctttcttttc aataaaacta ttgatattcc atgagcgaag agatattttt tgttcttcag
    12001 ataatggaat attttttaca ggaaagacaa ggcaggcaat cgtaacaaag aagaccagta
    12061 caattgaaaa ccagataatg aaacgaaaat cagtcatatt cagcaaaatc attcctaaaa
    12121 atggcccaac agcggcagct aaactagtac ttaatccata ataattgatg ccttcccctt
    12181 ttttagatgc agggatatat gcagtaacga tagcatttgt agctgtagaa actgtaccgt
    12241 agccgaatcc attcaagaat cgaactaaat acaatgcccc gatagttggc gtataaagat
    12301 atgctaatgt tgaaaggaga tagaagatcg cgccgcctct taaggtaaac ttccgaccaa
    12361 atagttctaa ctgtttcccc atgaataagc gtgccagcaa tgttccgatg atatagatcc
    12421 cagaagcaaa acctgcttgg ctcaagctgg cgtgtaaggt gtcttgcgca atgactgcca
    12481 tgatgaccat caacagataa taaatcaggt agacaataaa attgatcaat gtgattccaa
    12541 taaaatattt attaaataat ttcttttcca tgtatgttat acattcccct taagctgaat
    12601 atgcttttaa tttttgtatt tgaacttcta catcgtgata attggatgga tagcgatcaa
    12661 caagttggcg aataaataaa tctcttgtag acataaacag atttaaaaat tcttcgcatt
    12721 cgtttgttgg attctcatcc actgctttga tcaattcgat cgagttttga tacgcatcat
    12781 ttaaaaagac taaagaagct ttcatatcaa atcttgtttg atatgctgtt tctaatcgat
    12841 taatgattgt gttcatccag taattcatgt tttttccctc tattcttaaa aaattttact
    12901 tctgcagtat aacgaataaa aaaaaggaaa aaaatcgcat aagtgatgga aaaaggtact
    12961 taacaaatgt taatatcatt agtaaaatac ctttggactt taaaaagttt taaagtactg
    13021 tatgatcttt ttagatgaaa gtttggatgg gggagcatca ttggatagta gagtcttaaa
    13081 ggaatatttg gataaaaatg attttccgat tatacgcaaa ggatatcata aatatttgac
    13141 attcgaaggg atggaagatt cccatacgta tatcttgaaa gagggaacgg taaaagcaag
    13201 tgttatttca aatgatggga gagaatttaa ccttcgctat atcaaagatc ttgaaatcgt
    13261 ctctctttta aaagacgaat attcacaatt catcgattcg ccgtataata ttcgagttga
    13321 atcagaatat gcagagtttt atcgaatcaa tcgtgtgcgc ttttggcaaa atatcaacca
    13381 agacccaaag ctacaacact atgtaaaaga gtattaccgc tatcgtctgc tttattcgat
    13441 gaaaaaaatg cagcaaatgc tattaaacgg aaagctggga gctgtttgta cgcaacttta
    13501 cgaattatat gatttattcg gtgtgcggac agaagaaggg atgctgatcg atttttcgat
    13561 cacgaatgaa gaaatcgcca aattttgtgg catctcaact gctagcagtg taaacagaat
    13621 tttaaaacaa ctgaaaaaag atggtattat caccgttaaa gaccatcata tcgttatttt
    13681 gaatatggac ttgttagaag acaatattat cttttgatga acaaagaggt tgtgaaatca
    13741 gcgttttgac ctgagtatta gagaaacaaa cggaaaaata gttcctcata ttttgccgtt
    13801 ttttgagcta ataccgaggg tcagctgatt gaacaccgtt taccaagagg ttgtgagcct
    13861 gccgtttaac tctgagtcac aaggaacaat ttcaaaaaac agcttttcat gtttgatgaa
    13921 tattgtgact tgtgaccgag gagttggtag gagaacaccg tttaccaaga ggttgtgaaa
    13981 aagctgttct gcatcaagta ataagaagag ccaagtaaaa acagtctctt atgtttttaa
    14041 cttggctgga cttatcaccg tagatgtaag cttttgaacg ccgtttattt gcaacaaaag
    14101 gggctgtgac acgatttttg tcacagccct ttttttgcga acattcattt agcaattttt
    14161 agaaacttct gccgccaccg ccaaaagtcc cgccaccgct agaatgggta gtacttccgc
    14221 caccgccgct tccaggaggt gggcttttgg ggattcttct tgtcgtgaca aaagaatttg
    14281 tcagacgatc ggttttttca gttaatttga tactggattt ttctcggaat ggatatttgt
    14341 agatgcctga tttcaattga taacgcgaaa cagtcacaaa ataaaagaca agactaagga
    14401 taagagccaa aacaacagca atgatgattt ctactgtcgt caaaacctta tagcgtgtaa
    14461 ttttgccagt ttcttcgtct acacgataat gtcctcctgg aactccgtct tccacatatg
    14521 ttgctgattt agacaggtaa gcttttgcag cattgtaata ggcagcattt gtcatctgat
    14581 cataaacatc atctaagatc gaatcgattc tgctatcagt caaataatcg atcatattac
    14641 cagaagtcga aatgtagatt tcacgctgtc ccatatctaa tagcaatact gagccatttt
    14701 gatcatttcc gactgcgtct cttaagtaat tatcagcaaa gtctcttggt tcttctgaat
    14761 tagatgtagt cgttacgatc aagactcgtc ctttgatttt ttcattcagc ttttctgcct
    14821 gttgttccaa agattggatt tgatctgttg tgaataaccc agcctcatca tttaccgtag
    14881 ctgtatctgc atgagtagcc accggccagc caacacttac tagcaaaaga aagaataaaa
    14941 aaaatttttt catcatatca agtaacctcc gatcagaaag aggatcgcta gcccagcgaa
    15001 aatcccaaga gcggtcagtc ccaatttttt ctggctgatc ggcaggacac cgctgacctt
    15061 tcctgtttgt ccattcattg cgtagtagta gactttttta cttgagtcat tgcttcgata
    15121 agtaacaagc cataccggca gtaatacgta attatttact tcgtttgtga ttgaagcaga
    15181 agttcgcaca ccggtcaatg ttgtatatcc atttgcggta tcttttaaca gcttctctga
    15241 atagtctttt aattcagatt gaatctgtga tttgattgcc tcatattcga tatctctttt
    15301 ttctgcttgg aacccggcta aatactggct tttaaaatca attgctttat ctaacgggaa
    15361 aggttgcacg ccttcgacca tcttttgttg cgtatttttt gataaggcat tttttactag
    15421 ttctttaaaa gaaagcttgc ctgctcttga aacagcaaat tgcttcgttt ctgtgtattc
    15481 gatgtcacct atccgccata tgcggataac tgtaccattc gcctgcatgg caccatctac
    15541 ttcagcatct gtagcccaat aagggaaata cacacctgtc aacttatcga tttgatcctt
    15601 attgaaaaaa gctttgggaa caaaccattt tttcttggtc caagcgagaa atctgttgat
    15661 tgcttcttct ttctcgacag caaaaggaag tactttattt ggcaagaatt ctccgcttaa
    15721 acgtcccgat agtacgacgg gattatggca gtagtaacaa taagtcgcag cagttgtcgc
    15781 atcagtcacg atctctgcac cacagttggg acaaagaaaa agatctatcg ctgcggtatt
    15841 atctgcagat tcgtccgctg tatctgaaaa accgcctgcc tcgtcaaccg ctttgcgctc
    15901 ggcttcattc atatcggcaa gctgttcttg ggctgtaaaa gtaaaatctt cggtaggtga
    15961 aggttcttct ttgctttctt cgccagccat acgcgcttct ttttgctttt gctcatattg
    16021 gctgacttct tcttctgtat agatattcag gcagtattcg caatgaaatt tttggtcttt
    16081 gggatcaaaa gtcaacggac cgccgcaatt aggacattta tgtgtaagaa catccatcag
    16141 ctgcacacct ttctaaataa attaattaac ggggattcct ttaaacggtt taccacagtt
    16201 tggacagaat ttagggatgc cgttggaaag atccacgatc tcattgcatt cactgcatcg
    16261 catttgtagc ttaggctgaa cgttttctac aggtttggca gctccgcagt tggaacagaa
    16321 ttttccggaa ttttctgtcc cgcaaactgg acatgtccaa gtatccgcat ttccttgtgc
    16381 agtttgttga ttttgttttt ctgcttgttg tttgatttgt tcttggttat tttgagctgc
    16441 ttgagaaaga taagaaccat ttgcattcat tcccatacct acacccataa agccggtcat
    16501 tgctcctgct tcattttttc cggctgcttc cataccacgt gcaatggaac cttgcacgta
    16561 tccttcacgt acgctaggat cacctagcat cgccccttcg ttacgcatat tgatcagttt
    16621 cacagaatca tcggtgtaag aaatgctggc tacagccaca gaaacaatct ccatcccacg
    16681 taattcacgc caagaatcat ccaatgcagt atccatgtat ttgcttaatt ccaaactctt
    16741 agatggtaca taagaaatcc gttgaccatc tgcagacatc tgattgattg ctgattgtag
    16801 cgctgttagg aattcagcta aatactgctc attgatatca ttgatttcca cttgtgtttt
    16861 gtttttaggg attgcgtttg tataaaataa aatcgggtca acgacatgaa tagaataagt
    16921 gccatgtgcc cgtaagaata attctgcgtt atagaaattg tcaaagtagt tcaacggcgc
    16981 gcttgtaccg aatttgattc cctttatttc ttggagattg atataaaaaa cttgttgttt
    17041 ctgcggtgtg acgccgccaa atttgaatcg gtcaaaagtt tcagcaattg cttctttcag
    17101 tgttccattg aacatggaag gagctgcgtc atttttaatg gtgtaataac cttcctcagc
    17161 ggtataatcg atgatttttc caccatcgac caaaagcatc atcatgtttg gatagacatg
    17221 gacgactgtt ccgtctgtta aaacatcttc tgttccttta cgattggagc cacgtttgtc
    17281 atctttgcgg actttgacac cttttgtcat gaccgttgta tcactcatat tgtctggttc
    17341 gatgacttct agccattgat ctgccagacc gccgccaacc atacttgttg ctgctttgat
    17401 aagacccata aaaaattcct cctctgattc agctagtgcc aaacaattat tgtttattta
    17461 ttataccata ggaagatgtg aaacttctcg gactagagac ggaattgaaa tggaaaattg
    17521 cgtattgttt aaatacctga aaaagccgag aaggatagta gtttttttac ctaatatcac
    17581 gtataataag agatagtgaa ttttttaaaa aagggggatc cttatggcat atcaagcact
    17641 ttatcgagtc tggcgttcgc agcgtttcga agacatcgtt ggacaaaaag ctgtgacaca
    17701 gacactaaaa aatgctattg tttcccacaa aacctcacat gcctacttgt ttacaggacc
    17761 cagaggaacc ggaaagacga gtgcagcaaa gattttcgca aaagcaatca actgtccgaa
    17821 cagtaaagac ggtgagccat gcaacgaatg cgaaatgtgt cgatcaatta ctgctggtac
    17881 gcaagaagat gtgatcgaaa tcgatgctgc cagcaataac ggagtcgaag aaatccgatt
    17941 tatccgtgat cgggcaaatt atgcgcctac aaaggcagcg tacaaagtgt acattattga
    18001 tgaggtacat atgctttcaa caggagcttt caatgccttg ttgaaaacat tggaagagcc
    18061 aaaagaaagt gtcatattca tcttagcaac gaccgaacct cacaaaatac cagctacgat
    18121 catttcacgg actcagcgat ttgattttaa acggatcaat acggcagata ttgtcgaaca
    18181 tcttgaattt atcttgaaag aatcgactat cagctatgaa ccagctgcat tgaatgtgat
    18241 tgcccgttct gcagaaggcg ggatgcggga tgcattgagt attcttgatc aggcaatctc
    18301 gtttagtgaa ggaacaatta ctttagatga tgccatgcaa gtgacaggta gcttaactga
    18361 cgaaatgatg gacagttact tgagtgcttg cgtgaaaaaa gaagtaccag aagcattgga
    18421 aacattgaac caaattctcg cagccggcaa agaatcacgt cgctttctag aggatatgtt
    18481 acaatactgc cgtgacttgc tgatgtatca gcaagcacct aacctagttg aggaacaatc
    18541 gagtacgatc agaagtacat ttacagaatt ggcacaaact gtttcagcgg atcagctgtt
    18601 ttcaatgata aagattttga gcgatactca aaatgaaatt cgttttacca atagtgccgc
    18661 tatttatttg gaagtcgcta cagtcaaact tgcacagcta ccgtctgctt ctgttccttc
    18721 agcagatgta gcatttgcag atgatcagat agtaaaacag ctacaacaag agattaagga
    18781 actaagaaac gaaatcaagg aactacgcag taatacagga attgagaata caaaagtaca
    18841 gccggtacag caaacaaatc ataaaaaaca gacaagtacc tatcgtgtac cgacggaacg
    18901 agtatataaa gttctgcggg aagcgacaag acagcatctg aaccaagtaa aagaagtatg
    18961 ggaagacttg ctgatgagtc tatctgtcac tcagcgagca atgcttaaag caagcgaacc
    19021 agttgcagct ggacctacag gactagtgat tgcgtttgat tatgagattg tttgtcaaag
    19081 agcagcaaat gacgaagaat tgcagctttc ggtacacaac aacttaagcc gaatgatcaa
    19141 agattatgcg cctgatcctg tttatattac aagagaaagc tggccagaac ttcgaagaaa
    19201 ttatttgacg caggcacaag aagagggaac agaaaactct tttgcttctg aagactcaga
    19261 aggagaaatc gagttgcttc ctcaagaaca agaagtacca gaagaaacac ttgtagtaaa
    19321 agcagaggaa ctgtttggct ccgaagctgt tacagtggta gatgattaat aaaaaggagt
    19381 agataattat gatgcgtgga atgggcaata tgcaaggcat gatgaaacaa gtacaaaaaa
    19441 tgcaaaaaga aatggcagaa gcacaagagc tgctaaataa aacagagttt acaggtaaag
    19501 ccaccaatga attggtcaaa gcggttttta caggcgatcg tcgcatgaaa gatatccaaa
    19561 tcaatgaaga agtcgttgat cctgatgata tcgagatgct tcaggatctt gtcgtaatgg
    19621 cagtcaatga tgcgatcgca caaattgatg tggaaactga gaaaaaatta gggaaatacg
    19681 caaaaggttt gccaggattt tagttttaaa ctgaaggagt aatgaatatg caatatccag
    19741 aaccaattgc gaaactgatc gacagctaca tgaaacttcc aggaatcggt cagaaaacag
    19801 cgacaagact cgccttttat acgatcgata tgaaagatga agtagtaaat gaatttgcta
    19861 aatctttgtt aagtgtaaaa cgcgatttgc atttttgtag tatctgcgga aacatcacag
    19921 aagaagatcc ctgcgaaata tgtagaaatc cttcacgtga tcgaagcgtt atcctagtgg
    19981 tcgaagagcc gaaagatgta atggcaatgg aaaaaatgcg agaatatcac ggactttatc
    20041 atgttctcca tggtgtgtta tcaccgatgg aagggacagg tccagaagac atcaatattt
    20101 cttctctgtt gcagcggctg catgatgcag atgtaaaaga agtgattatc gcaacaaatg
    20161 caacaacaga gggagaagca acagctatgt atctctcacg tctgatcaaa cccgcaggta
    20221 tcaaagtgac tcgtctagca catggtctgt ctgtaggctc tgatatcgag tatgcagatg
    20281 aagttacttt attgaaagct gtcgaaggaa gacgtgagtt ataaatttct aattaagtgt
    20341 tttttcagat acaatataaa agcagcacaa gagatggggg caacaatgtg aacggactat
    20401 ttattacaat cgaggggcct gatggtgcag gaaaaaccag catcttgaat gaactttacc
    20461 ctcttttgaa agaagtagca aaaacagaaa tcattcaaac aagagaacca ggaggcattc
    20521 cgattgctga agagatccga gcagtgatcc ttgatccaaa aaatgatcgg atggatgaac
    20581 gtacggaagc actgctttat gcagcagcaa gaagacaaca tctagtcgaa aaggttctgc
    20641 ctgctctggc aaaagggaaa atcgttttat gcgatagatt tgtcgacagt tctcttgctt
    20701 atcaaggtgc aggccgacgc atcggcgtta ccgagattgc tcggttgaat gagtttgcta
    20761 cagaaggtac tacgccggat tttacccttt atcttgacgt agattcagac accggattaa
    20821 gaagaatcaa gaaaaacaga caaaatcaaa tcgaccgttt agattcggaa ggtttggaat
    20881 tccaccagcg tgtacgtcat gcttacttaa agctggcaga agagaatccg gaaagaatcc
    20941 acaaagttga tgcaagaaaa agctttgaag aagtgctgca aacaagttat catacaatta
    21001 ttgaacagta tccgcaattt tttgaaaact aggaaggtga acgtttatga aaattatttt
    21061 agcgattatc caagacaaag acagcaaccg tttatcaaat gaattgattg atgcaaatat
    21121 ccgtgccaca aaactatcat cgacaggcgg cttccttaag gctggaaaca gtacgtttat
    21181 cgtgggtatt gaagatgacc gtgtagaaga agcattggaa atcatcaaaa aaacgtgtga
    21241 atcaagaaag caatttgtat ctacacctgt tacactggat atttcgatgg atgggggcgt
    21301 cccttatcca gtagaagtag aagtaggcgg tgccacagtc ttcgtcttac ctgttgaagg
    21361 attccatcaa ttttaggagg agctgagcta tgaatcaaga aataaacttg gctcaaatgc
    21421 aaccgatcgt gtatcagcag cttcaacgga gttttgagca tgggcgtatc gctcatgctt
    21481 atctttttga aggggagaaa ggaacaggaa aacatgaagt aggtatatgg cttgcacagc
    21541 acctgttctg tacccaaatg aaagacaatc tgccttgtgg aaaatgcaac aattgtcagc
    21601 ggatcgaaaa gaaagaacat ccagatgttt tggttattga accggaagga caaaccatca
    21661 aagtcgatca gatcagacgt ttgcagacgg aattcagtcg cagcggttac gaatcaagaa
    21721 aaaaagtttt tttgatcaaa gaagctgaaa aaatgaatag cagtgcggca aacagtttgt
    21781 tgaaattctt ggaagagccc cctggagatt ttttagcggt gttagaaaca gatgcagtag
    21841 gaagaattct ccctactatt caatctcgtt gtcaagttct gcattttcaa gaactttcga
    21901 aagaagcact gatccatcga ttgcaagaag agcagattcc attggaaaaa gcaaaacttt
    21961 tggctttctt aacaaacagt ttaggaaaag cagttgaaat ctctcaagat gaatggttta
    22021 atgatgctaa ggacttgatt tatcaatggt tcgtttactt gcaaaagcaa gatactcaag
    22081 cctttatcta tgtccaaaaa aaattggtaa aatcatttaa agaaaaaaca caacagttta
    22141 caggattatc tgtcttgatg ttttattatc aggaagcaca gcgtcaagca gtagcaaacg
    22201 gcgaatatgg caaagtgaaa cagataaacc agataattga acggatattg atcgcagagc
    22261 agaaactacg cagcaatgtc agttttcagg ctgttgcgga acagttcgtt ttgcatacga
    22321 ttttttagct tgccgcaaag gatgatgatc aaggaggatg gaaatggtag aagtagtagg
    22381 tgtccgatat agagatgcag gtcatatcta ttattttgca ccaggagact ctgaatatgt
    22441 ttatagtgac aaggtattag tcgaatctca gcaatcctta catattgcta ctgttgtcct
    22501 tcctaaaaaa gagattgatc ccaatgacct tcctgaagct atccgtccga tcttgcataa
    22561 agcgtcacaa gctgaattag aaaaagacaa aaaaaacaaa aaagatgctt atgatgcatt
    22621 gaaaatcgca aaagaaaaag tgcagttgcg tgagttggaa atgaaaatgg tcaaagccga
    22681 atacacattt gatcgaaata aattgatttt ttctttcaca gcggacgggc gaattgactt
    22741 tagagaattg gtaaaagatc tggccgctgt atttcgtact cgtattgagc ttcgacaaat
    22801 tggtgtccgc gatgaagcaa agataatagg cggaattggt ccatgtgggc gtccattatg
    22861 ctgttcttct tttttaggcg atttcgtgcc tgtatcaatc aaaatggcaa aagatcaaag
    22921 tttatctttg aatccaacaa aaatttccgg tttatgcgga cgcttgatgt gctgtctgca
    22981 atatgagagc gaagaatacg agacagcgaa aaaagaactc cctgactttg gaaaagtaat
    23041 tgctacacct gatggtaaag ggaaagtcgt agggttgaat ctgcttagcc gtatcgttag
    23101 tattcggctg actggacggg aaaatcctgt cgaatatgaa tgggatgaat tgaaagaatt
    23161 tatccaagta ggtgagatga atggataaga gatcattata cgacggattg aatcaattag
    23221 aatcagagtt gagaaataca ttaacagaac ttgttgaaat gaagcatgct ttgcatgaga
    23281 tcgtagaaaa aaatacaact ttggagatgg aaaatcaacg tctgcgggaa cacctgcggg
    23341 aattgaacca agttgcacaa gctccagctg aaaatgtgaa gcaggaactt tctaagtctc
    23401 gtatgaatct tgaaaaaatt tatgaagaag gattccatgt ctgctacgat ttatatggat
    23461 caagaaggga caatgacgag ccttgtgcgt tttgtctaga agtgatctat cgcgaaagcg
    23521 gtcgatgacc aataacaaga ggagactgga gcggagaaat actgctccag tctttttatt
    23581 aaggagattg tgacagatgt ataatcaaaa aagtttcaag ggaaatccgg tatatgggga
    23641 tttatatctt gtaccgacgc caattggaaa tttagaagat atgagctaca gaagtgttcg
    23701 tatgctgcaa gaatccgatc ttatcgcaag tgaggatacg cgtaatacac aaaagttatt
    23761 gaatcatttt gaaatccata caccccaaaa aagtttacat gaacataatt ataaagaacg
    23821 tgtccctcaa ttgattgctt tgttaaaaga aggaaaaacg attgctcaag tcagtgacgc
    23881 aggaatgcct tccatcagcg atcctggaca tgagctagtt ttagcctgca taaaagaagg
    23941 gatttctgtg atctctatcc caggacctac agctgggatg acggctttga ttgcttcagg
    24001 gctagtgcct caaccatttt tattttatgg cttccttcca cgtaagaaaa aagaacagaa
    24061 agaaacattg aaaaagctaa aagaacagac agttacgctg attttttatg agtcacctta
    24121 tcggatcact gctacattga cgaatttctt agaaattttt ggaaatcgcc aagtcgtttt
    24181 gtgtcgggaa ttgacaaagg tccatgaaga ataccttaga ggaagtacgg aagagctact
    24241 cgcttatatc gaagaacatc cagtaaaggg tgaatgttgt ctgcttgtag aagggaattt
    24301 gttcgaaatg ccagtgacgg aagttgatga agagttggga acgttaaagg aacaagtaga
    24361 agcaaagatc gcttcaggcg aaaaaccaaa tgctgctatc aaagcagtag cgctgaaaaa
    24421 tgggctgaaa aaacaagaag tctaccgtca gtaccatgaa ttagactgag atagtataag
    24481 ttgtctataa tgaaagacta atgaggaaat cagctactaa cttttcaaat ctcttgaacg
    24541 cgaacgaatg ttcttgtata atgaataaga ggtgaggatg atggagctgc aatttccttt
    24601 acataatgat acatcaagaa aaatcattca tattgatatg gatgcttttt tcgcttcggt
    24661 tgaagaacga gatcatcctg agtttcgcgg acatccatta gtaattgcca gacaccccag
    24721 tgacactggt gggcgtggag tagtgactac agcaaattat gaagcaagaa aattcggaat
    24781 tcattcagca atgagtgcac aaaaagcgta tgagctttgt ccgcaagcca tctttaagcc
    24841 tggcgaccat cataaataca gcgatatttc caaacaagta cgggaagtat tctatcgtta
    24901 tacagatatt gtggaaccag ttagtatcga tgaagcgtat cttgatgtga ccgaaaataa
    24961 aatcaattgc aagtcagcca tcaaaatcgc ccgaatgatc caagcagata tttgggagag
    25021 tgtccagcta acatgttctg cgggagtaag ttacaataag tttttggcca aattagcatc
    25081 agactatcaa aaacctagag gaatcacggt agttgcccct caagacgctg tgccattttt
    25141 aaaagctttg ccaattgaaa aatttcatgg ggtagggaag aaaactgtac ctagaatgca
    25201 tgagctaggg atttatacag gaaaagacct atatgaatgc acagaaatga tgttgattcg
    25261 aaattttgga aaaatgggct attctcttta tcgtaaagtc agaggaatcc atgattctcc
    25321 agtaaacgtt acaagagagc gaaaatctgt gggaaaagag catacttatg ggcagccgtt
    25381 gcaaacggaa gaagcagtgc tgactcagtt gcgccaacta gctgaaaaag tagaagaagc
    25441 actgagaaga gtccaaaaac atgggaaaac agtagttttg aaggtcaggt acacggacta
    25501 ttctactgtg accaaaagag tgacattgcc agaatatatc tataaaaaag aagcactctt
    25561 ttatcaagca agccttatat gggaggaaat cttgggagta gaaaaaggaa tccgattgtt
    25621 agggattact ttgacgaatc tggacccgat gacttatgaa aacatcgtcc ttccattatg
    25681 ggaaaatcaa gaaatataaa aacagctttg agagttgaaa ccggaaatcc gttttctctc
    25741 ccaaagctgt ttttatatca agtcttgctt tttgatttgt tcataggttt ctgttgcatc
    25801 cgtacatttt tcccaaatca caacttgatt ggattctaaa gatttttgga cgtcaatgat
    25861 ccgctggttg ctacttccgc gaaattgtag ttttaggtca cgtttgctta attcaaagcg
    25921 tccgtcaaca aggatatcga tttgcgaaag gagttctaat ttatcttctg aatcttgtag
    25981 caattcatca aaggtatagc ctgtccatga ccagatatct tttgtcgtac cgaactcttt
    26041 tcggatacgt cgtacgactt gcagacaaac atctgtattc aaaaaaggtt cgccaccaag
    26101 taaagttaat ccttgcacgt aatcgttgcg caagtcagcg atgatttgtt cttctaattc
    26161 aggtgtgaat ggtttgccat atcggaaatt ttgtgcagct ttgttgaagc agccttcgca
    26221 ggcaaataag cagccactaa cgtaaagact gttgcggaca ccttcaccat cgacaaaatt
    26281 aaacggtttg tagtcagcaa tatagttttg gctgtagtct tttgctaacc attcttttgg
    26341 ttttggattt ctcataaaaa aacttctttc atttaaggtt caaagatata gctattgaag
    26401 ataaaatcta ctcgaccaca taaataagaa caaactgacc taaatccatt gttcaagaaa
    26461 gcttgttctt attttgatag agggcgttca aagatacaaa gccatcgaaa gagggtggtg
    26521 aaaaaagact tgtcatagcc tcttattctg aataaacggt gttcaaaagc ttgcatctgc
    26581 agtaataagc ccaactaaac gaaaaatatg aggaactatt tttgtttagc tattcttatt
    26641 acttgatgca ggacagcttt ttcacaacct catttcatgt gtttcacacg tgaggaaatt
    26701 tctttatggc ggccatgtac catcggacga gcttgcggat ttcctaaata gccacaagta
    26761 cgtttgacta catcacatgt ttttggatca tgatttccac attctggaca ttcgaaccct
    26821 cgttctgtcg gtttgaaatc accttcgaaa ccacattgat agcaatgatc gattggcgta
    26881 ttcgtaccaa gatacccgat tttgtcatat gcaaaatccc atacagcttc taaagctttc
    26941 ggattttgtt ggagcatagg gtattcacag tagtggatga atccacctga acaatatttt
    27001 ggataatcct tctcaaattc caatttttca aatggcgttg ggtttttacg cacatcatag
    27061 tggaaactgt ttgtataata ttctttatct gtgatatttt cgatgatgcc gaatttttct
    27121 gtatccaaac ggcagaaacg gtcagttaga ctttcacttg gagttgaata gacactgaaa
    27181 tgataaccat attcatcccc ccatgcatct gcattagctt ttaattcttt gacgatatca
    27241 agtgtgaaag cttttgcttc tgggtttgtt tcccattcgc caccgaagaa agctgaagca
    27301 acttcataca atccaatgta gcctaacgat acagtagctc gacggttttt gaataattca
    27361 ttgacagaat cggtacgtga aagacgtttc ccgaaagcac catacatata taaaattggc
    27421 gcatttgctg gaatggcttc tttacaacgt tctacacgat agagaagcgc atctttcatg
    27481 atttctaagc ggtcagacag taactgccag aatttattct gatctccttt tgattcgatc
    27541 gcgattcttg gcaaattcaa tgtaacaaca cctaaattca ttcgtccgac atttacttct
    27601 tgaccatttt cgtctttcca cccttgcaag aatgaacgac agcccatagg tactttgaaa
    27661 cttccagtta attcaacgat tttatcgtaa ttcagaatat caggatacat tcttttcgtt
    27721 gcacattcca atgctagttg tttgatgtcg taattaggat cttcagggtt taagttcaca
    27781 ccttttttca aagtgaagat cagtttaggg aagatagctg tccgcttttc actccctaat
    27841 ccattgattc gaatctgtaa aattgctttt tgaatttctc tttcaaacca gttttctcct
    27901 agtccaaatc ctaatgaagt gaaaggagtt tgtccatttg aagtaaataa ggtattgatt
    27961 tcatattcca aactttgcat agcatcgaag atatcttttt tggtttttgc ttttgcatat
    28021 tctttttggc gttctggcga atcgatccat tcttccgcgt cttttagatg ttttttatag
    28081 ttcaattctg caaaaggagc aagcaattca tctgttcggt cggcagaaca gccaccatat
    28141 tgacttgacg ctacattggc aataatctgt gagatttgtg ctgtggcggt ctggatagat
    28201 tttggcgatt cgacttcggc attgccgatt ttgaacccat tgttcagcat gcctttgaaa
    28261 tcaatcaagc agcaattcgt catcggtgta taaggatgat aatccaaatc atggtaatgg
    28321 atatcgcctt tttgatgcgc atttgctata tgtggtggca acattttcag tccgattgat
    28381 tttccaacga tcccggctgt cagatctcgc tgtgtattga aaacattact gtctttattt
    28441 gcgttttcgt tcacgacagt ttggtccttg ttgattagct tttcaatcga aaagttgata
    28501 tctgttgctt tactgcgagc aaaatctcgt ttggtacgat aattgatgta ctcttctgct
    28561 aattcatatt cgtttttttc tagtaatata tgttccacga tgttttgaat ctcatagatt
    28621 ttgacatttt cagcaaaccg gcttttgatt tcttggacaa ccaagtctgc aatcatttga
    28681 attttttcat aagtaagtgg tgataatgag ccgtgaattt tcttttctgc tttgatcagt
    28741 gcttcattga tcttttgttc atcgaaatca acatggcggc catcgcgttt gatgactttc
    28801 atctgggatg tctctggctg ctgagtaacg acagtttttt cttcataatt tactttcatt
    28861 atcttcaact cctgctaaat tgttcttgtc tatcataaaa caaaatcggg gaaggagcaa
    28921 ctaaatatcg gctatatata gtattcaatg tgctgtataa ttggatttct atactatatg
    28981 tagtgtatat tttattgtga aaaaaggtga aaacttgtag ctggtggatt tgttttttgc
    29041 atatttaatt ttgctgaata aaataattga ataaaatttt ttattcagtc caagtaaaag
    29101 tgacaaaaac ataagagcaa attattcgta gatatgctac tatgcaaata aatagaaatg
    29161 aaaaatgaaa gaggaggaga agaattggaa aagatgatcg aagtgaaaca tctaagtaaa
    29221 agctacggca gcaaactttc tcccgtcaaa gccttagata atatctcatt cacagtagaa
    29281 gaaggcgagt ttgttgggat tatgggccca agtggtgcag gaaagacaac gctgatgaat
    29341 atcttgtcca cgatcaattt gcctacgatg ggcagtgtct cgatccaagg aaaagaaatc
    29401 acaaaaatga aaaacagtga gctcagtgac tttcgtcgaa aaaaactagg attcattttt
    29461 caagagttca atttgatcga taccctaaat gcaaaagaca atatcctatt gcccctagca
    29521 gttgaacgga tgactaaaga ggaaatggaa aagagagtaa gacatgtagc tcaattgttg
    29581 aatatagaag aactgctcaa acggtatccc gatgaacttt ctgtcggaca gagacaacga
    29641 attgcagctg ctagagcact tgttgttcag ccacaagtga tttttgcaga tgaaccaact
    29701 ggctctttgg actcgaagtc ggctacagaa ttgttgaact acttgaagac catgaatcaa
    29761 aaagaaaaag caacgatact gttagtcacc catgatccat atacagcaag ctactgcgat
    29821 agaattttat ttattaaaga tggtgtgatt ttttctgaag ttgtcagaag agggacaaga
    29881 agagagttct ttgagaaagt aatagacatg caggcgacga ttggcggagg aggaaaaatg
    29941 aatgctgtat aaactagccg ttaaaagttt ttttcggcag tcacgaggct atcttgttta
    30001 ttttttcagt ttgactttat ccgtaatgat ctattattcc ttcagcgcaa tgacctacga
    30061 tcaatcttta gtcagaagag caagtcaaga tacttcgata gatggtggat tgagttttgg
    30121 tggatttatc attactgttg ttttgctttt tttcgtattc agtgccaacc gatttttctt
    30181 gaatagaagg caaaaggaaa taggcatcta tcagcttttt ggtatgaata aactgcagat
    30241 atcaggtata tatgtgttag aaatcatgat aattggattg ttcgcctgta tttctgggat
    30301 attactcggt attatttttt ccaaactctt ttcaatgatt ttggttcgaa tgatggatat
    30361 ggatcttact agtccatttt ttatctccat tccatcagta gtagatactt tatttgcttt
    30421 tttcatcatc cttttagcag tatctgtata ttcaatatgg aaaatatggc gctatccaat
    30481 gattcgaagt tttggagaaa aagaacaagc agatagttca acaatgcgct tcaggacaag
    30541 gcaccgatta ctcgcagtca tcgggttact acttttaacg agcggatatt ttggagctag
    30601 tcattttagg gaaatcactt catggatgat cagttatcat tatcttgata cattagtttt
    30661 cttttttcct tttttaattt ttttcgtttg tgtggtcggt acttatttgt ttttctgtta
    30721 tacatttcgg ttatttttga tcatattgag taaatggaag atttattact atggtatcaa
    30781 ttttttgata atgggtaaca cgcaagttca tttgctcaaa agttggcgta caaattctct
    30841 gattacagtc attctcggaa tttctttaat gacaattggc ggcacggcta gtatctctac
    30901 tattttatcc taccaggaga aagtttcctc cccgatggac tatcagtttg atgttgagac
    30961 agagaaaaaa atcaagccga ttctagcaga ggaagggcag aagataacta aagaaatcac
    31021 cttgcattat aaagtcattg ggagtatcta tagtcaaaat ctcgaagaac ctatgtttca
    31081 aactgctaat ctgatcacgg aaacacaata tcaagccttc agaaaagtta atccagattt
    31141 gccgaacatt tctttgaaat cacctggtca tactgttttg cttgatcaaa tgcaaactct
    31201 ttttcggaat atttcattat atggatctag tattggactg ccagataatc aagaattgaa
    31261 gattcaacaa atgcttccaa gtgtattagg tgacgaaaca atggtatatc taagtcctgt
    31321 actgattgta gatgataccg tttttgaaaa agcaaaaggt gtggattatc aagtagtcaa
    31381 tgtagatgta tcaggtggaa ataatgaaaa aatagatgaa cgactgagca atgacctgtc
    31441 gataaagtgg ctcaatgctg tttattattc ttatgatatc gtaaatggaa gtctacaagg
    31501 agaaattagc acaaagaaac ttccggatga ccaaatgaag gaagcacaat ttagccaaga
    31561 aagctctcgt ttgaatttct cttccagcta ttcgaaattg cgtgcagtta atcggcgaat
    31621 cggtatcaat atttttgtca cactatttgt cgggatgatc gtgattattg ctactggtag
    31681 tattttgaca gtccggcaat tagcagaagc agaggaagaa aaagaaaatt atcacctatt
    31741 gactaagctg gggattccac aaagaaggat cagacggatg atcttcgagc aaaacgcact
    31801 taccttcttt ccacccatga tattagggat cactcacgca gtatttgcaa tcaatgtctt
    31861 cactcagtat ataaaaactg ccgattactg gttggcttat cttgtgagtg gcttattgat
    31921 ccttatctat cttttgcttt atgtcattac gagtcagtta tattgtcgga ttatcgagga
    31981 atgatatacg gaataagaca agataataaa tagaactaaa ttttgaatac catttatttg
    32041 gaataagagg ttgtgacacg atttttgtca caacctcttt attttttata ttttttaaaa
    32101 gtacgagaaa ttataaagaa aaaagcagtg atttgatgtg ctatttttat aaaagctatg
    32161 tgacaaaact gttagtaaaa aaacggtttc tgtaagctag acacatgttt ctttttgtca
    32221 aaattagtta cactttgtgt gaggttatat gaggagggaa tccaatgaac aaatggcgtt
    32281 ctaatccaaa attgaattat tggggaaaat tcatcattcg aacaattttt tatagtgcag
    32341 tactgctgtt cctgatttat ctgtatcact ataaaaatat tcaaggagga acttttatct
    32401 ataacgaatt ttaggaggaa gagtatggaa atcaaaacga ttattgaagc aatcgatgat
    32461 tggggaataa atgaacctga tcgagttgct tatcaggcgg atgagacgca tacttttggt
    32521 gaattgaaac aagcatcaga cgcacttgct tattatttag aaaaaaaggt agaaggcgat
    32581 ggaccaattg tcgtttttgg caatttggag tttgaaatga ttgtctcatt tctaggggtt
    32641 gtcaaatctg gccatgccta tttgccgatt gaagagcata cacccaagga gcgtatccta
    32701 tcgatctttc gggtggctaa accatctatg gtcatcagta tcggggactg gatagaagat
    32761 attccttcgg ttcctgtcat aacaaaaaaa gaatttcaag aaatcaagaa gataccagta
    32821 ggattttatc ccagaaatag cgtaaaaggt acagaaaatt tttatattat ttttacttct
    32881 ggtactactg gagaaccaaa aggcgtacaa atcagtcatg ataatctagt cagctttgtt
    32941 caatggacga tggaagactt tgggatcaag cctggtatgc atttcttagc ccaagcgcca
    33001 ttttcttttg atttatcggt attttctatt tatcctgcat tagtttctgg cggaatgctg
    33061 aagccgttaa ataaagcagt tgttcaagac tttcgtcaat tattcgcgac attgccagcc
    33121 ttgaagttga atgtgtgggt ttctactcca tcatttatgg atatttgctt gatggaacct
    33181 acattcaatg gagaaaatgt gccggaattg gacatgtttt tattttgcgg agaagaattg
    33241 acgaaaaaaa ctgcagaaag tctacttgat cgattcccaa atgcacggat atataatact
    33301 tacggtccaa cagaagcaac tgtagctatt tcaagtattc agattgacca gcatgtacta
    33361 gatagttatg accgactacc gattggctac gtaaaaaaag acacgaaagt ttctattgtc
    33421 caagaaggta aacctgtttc aaaaggagaa acgggtgaaa tcattattgc aggaccaagc
    33481 gtatcaaaag gttacctgaa caatcccgaa aagacagctg ctgcgttctt ttgtcaaaat
    33541 gacttagcaa gttaccatac aggggatgca ggtagactag atgaatatgg gcttttattt
    33601 tatgaaggac gtatggatca gcaggtcaaa ttacatggat accggattga actcggggat
    33661 atcgaacatt atctgctcca agacaaccga atcaaacaag ctgtcgtcgt accaaaatac
    33721 caaggaacaa aagtacagca gctggttgct tttgtcgtat tagaaaacaa aacgaaacag
    33781 ccggattttc agttgactaa atctatcaaa gaacaattac tagaagttgt aatggattat
    33841 atgattcctc agaagttcca ttatgtcgat ctgctgcctc agacagttaa tggaaaaatc
    33901 gatcgaaaaa aattaacagc tgaggtgaat cctgaatgat gttacctcat atgatccctt
    33961 atgcagagcc gttttatttt tttcttttag ccattgcgct tgtaccgatc gttctatctt
    34021 tattgatatg ggaaaaaaga ttaccagttt atcaatcagc agtgacactt ttctttttat
    34081 ttgtcagctt tggaggtaat tattggcaac agggagcggc gctgattggg tatgttttgt
    34141 ggcagacgat acttgtatgg ggctattttg tttatcggca aaggaaaaat cagacaggat
    34201 ggttttttgc cgccgttatt ttagcgataa ttccactgtt tttaacgaaa gtgactcctt
    34261 tttttgaaca tggaaaagca tcgttacttg gttttttagg aatctcttat ttaaccttta
    34321 aatctgtaca aatgatcatg gaaataagag atggcatgat caaaacatat aagccatttc
    34381 gttatatcca gtttctgctg ttctttccaa caatctcttc tggaccaatc gatcgctata
    34441 ggagatttga aaaagatcag ctccatccgc ctgttcgtga taaatatgtt gatcttttgg
    34501 gaaaaggcgt ccaaaatatt tttgtcggtt ttttatataa attcattatc ggttattatt
    34561 ttggtcaagt actgcttcct gtagtgggac gcattgcctt acagcatggt ggtatttcat
    34621 gggcattagt aggttatatg tacgtgtaca gtatgtattt gttttttgat tttgctggct
    34681 atagcttatt tgcagtcggt acaagctaca tgatgggata tcagacacca atcaatttca
    34741 ataaaccttt tttatcatgg aatatcaaag aattctggaa tcgttggcat atgacgctgt
    34801 ccttttggtt ccgtgattat gtatatatgc gattgatgtt cttcatgatg aagaaaaaat
    34861 tttttaaaag caagatcgta atgtcaaata tcggttattt tgctttgttt cttctaatgg
    34921 gtatatggca tggattgacg tggtactaca tcgtatatgg actgtatcat gcttgtctga
    34981 tctgtctgac agatgcatgg atccgattta agaagaagca taaagagcag ttgccttcta
    35041 acaaatttac gcatggctta gccgtatttt tgacatttca agcagtatgt tttagcttcc
    35101 tgattttttc aggattttta gataaacttt tttttagcta acgaatagtg acatattaat
    35161 ttatataaca aaggagaaaa ggaaaatgga agaacaaatt ttatcaatct tagaagaaat
    35221 aacaggaaca gatgaagtaa gaaaaaatcg tgatattgat ctatttgatg aagggttgct
    35281 tgattcttta gcatctgttc aattattagt cgagctagac ggagagctgg atatccaagt
    35341 ccctgtatct gaattcgagc gagaggattg gagcacaccg aataaaatca ttcaacaagc
    35401 aactgcattg aagggttgag tatgaaaaag aaactttttg ctattttcgg cccggttgtt
    35461 ttagctgttg gcttactgtt tgtgtttttt acctcctcct atcaagtaga tatctctaat
    35521 ccagaaacga ttagacgtgc ctcttcttcc atgtcagtga acgtgttgaa aggagatgcg
    35581 ataaaaaatt atgcattatc tgaaaaacaa tatattcctt tttttggctc aagtgaatta
    35641 agcagaatca gtccatttca tccgtctgtt ctggcagaaa aataccagcg aaactatcgt
    35701 ccatttttgc tgggagcacc cgggacacaa tcgttaagcc aatatatgat gatgcgttct
    35761 gcaggagatg ctatgaaaaa caagaaagtc gtctttatta tttctcctca gtggttcgtt
    35821 aaaaatggtg taaaaactga ttactttaat acgtattatt ctgaacttca aacatacgac
    35881 tggttgtttt caatgaagaa agtaacgcct gctgatcgct atttagctag aagattatta
    35941 actttctcaa aagtaaaaga gaatgatacg ttgacggcga ttctgcaaac gatcaaaaaa
    36001 gggaagctac ctttacctga atctttgaat cagctgcgca gccaatggaa catgctgaag
    36061 agagaggatg aagtattcag caatattggg ttgaaagatc gacagcaaaa aatcgatcat
    36121 gagtcaaaac gattaccaaa acaataccaa gaaacagaat tatccatttt agcaaatcaa
    36181 attggagaac gagaaacgac gaataatcca ttcgggttga aaaacgattt ttatacgcat
    36241 cgtatccgag cccacgagcc agagctcaaa cagtcacaaa aaaattggga ttatcgtttt
    36301 tcgccagaat tttctgattt tcagctggtc ttggatcaat tagcaaaaaa ccataacgaa
    36361 gtattgttca tcatcccacc ggttaacgag aaatggagcg attataccgg actatctcaa
    36421 gaaatgttac aaggatttgc caaaaagatc aaatttcaat taaatagcca aggattcaat
    36481 cgaatagctg attttgtcaa tcaagcgggt accaactatt ttatggaaga cacgatccat
    36541 ctaggctgga aaggctggtt ggctgcagat caacagatca gaccgttttt agaagaaaat
    36601 catattactg ctagtaagta tcatcttgac gatgcttttt ttagcaaatc ttggcaacat
    36661 caaataccag ataaactaca attaaaataa agaaaacagg cgagtgttgc tcgcttgttt
    36721 tctttatttt tgaatattct gaataaaaga taaaaatacc aagtttgcct gaacaaaaat
    36781 cataagcaat ggtataatca caatatgaat atagaaatgg gggaaaacat ttggatacaa
    36841 aagaagaaca gctacttatt gatttaactt ctgcaaaagg agtacctgga aatgaagaag
    36901 aggtaagaga agtattcaga gaatatgcca aaccattcgc tgatgatatt ttttatgacg
    36961 gtttaggcag tgtgattgcc aaacatggtg ctggcggagg ccccaaggtc tttatttctg
    37021 gacatatgga cgaagttggt tttatggtaa caaaaataac cgaaaaagga ttcttggaat
    37081 tccaaactct tggaggttgg tgggggcaag tcatgcttgc tcagcaagtt gaaatcaaaa
    37141 cacgtgaagg gaaaatcgtc cacggtgtga ttggttccaa accaccacat gttttaacac
    37201 ctcaagtaag aaataaacct tatgaaataa aggatatgtt cattgatatt ggcgcatcaa
    37261 gccaagaaga agccaaagag tggggcatac gtcctggaga tatggtcact ccatatattg
    37321 aatacaaacg aatgaatggc agtaaatatt tactggcaaa agcatgggat aatcgtatcg
    37381 ggacagctgt ttctctgcga gtattagaga atctatctaa agaagcacac ccaaatgttt
    37441 tattcgcagg aagcgatgtg caagaagaag taggcttgcg aggtgctcga acaagtacac
    37501 accttgtaaa tcccgatatt gcttttgctt tagatactgg tacagcagga gacactccag
    37561 gaatgacacc aaaagaagca gattcgattt taggaaaagg cccacagatt ttgatttttg
    37621 atgcatctat gatcccgcat aaaaaattat tgaattttgt tattgatgta gcagaggaaa
    37681 tggatattcc attccaatat acagtgattt ctggcggagg aactgatgca ggacaaatgc
    37741 atttgacaag agatggtgtt ccgtctgtag ccattaccgt tcctgttcgt tatctgcatt
    37801 cccatacatc agtgattcat gaagatgatt atttgaatac agtgaaacta gtcacagaat
    37861 tagtgaaacg gcttgatagt gaaacagttg aaaaaattcg gagttatgaa taatgaataa
    37921 tagaatacct atttctgaat atcggaaaaa agcacgagtt tctttggaaa atcaatgggg
    37981 aatcaatact tggcttgttt tcttatctat aatatttacg agcgtcatcc agaacttttt
    38041 tgacggtttc agtccttttc cggaagaaag catgcagcag cacttattga gttttctagt
    38101 cgataattta cttttattcg cattcaacta tgcattgttt tacattgcgc tagtagttgt
    38161 acggggtggc agaccaaaaa gtaacctttt attttcagtt ttccaaagaa agtattttgg
    38221 ttcgatactt ggcatcaatt tgatcaattc agtcatcaac tggctactca acagtttaat
    38281 ctttctgccc atgtttttaa tcagcggagt gagtacttat atgcagctgc ttttcagttc
    38341 aaatgcggta cctacgggtg tcatgccaga agattttctt gatatcagct ttgttttatc
    38401 gatgataggg atgatttttg tttcagcact catcatgagt attgttggcg gtttattcca
    38461 atttgctgca tggacaaagt tcgactatcc tgatttgtcg gttattcaat gtttgaaata
    38521 tggctggttt ttattgaaag atagaattgg tacgtatatt ttgttgcagc tgtcttttat
    38581 cggctggtat ttgctaggag caatcgcttt gttcatcggt cttttgtggg tcattgttta
    38641 tgtgaatgtg tcagttgcag aattttatga acaagcacgg attgaaaaag gaggtccaga
    38701 cgcctatttt acttagcatg taagcagttt attcatcaaa aaaataactt ttgtggtagg
    38761 ataaatcttt gagggggaat gtacgatgaa attaactgtt ttaggatgct taggagcgta
    38821 tccttataaa aatcaaggga caacaggtta tttgcttcaa tctgacgggt tcaatttgtt
    38881 gatcgatgca ggtagtgcta cgctgatcaa attacaagaa caccttgatc cacttgatct
    38941 agatgctgtg atccttagcc attatcatca tgatcatatt gctgaccttg gtgtattaca
    39001 gtattactgg caacttcatc ctaagagaaa tccggagaca ttgctttcaa tttatggaca
    39061 cacaaaagat tccattcact ttgaagaatt gacgttggaa aatgtctcaa aaggacatcc
    39121 ttattttgaa gctgaagaat taaaaattgg tccttttctg attactttta tggaaacaat
    39181 ccatccagta gtatgttacg ccatgcgtat cgtagaagaa acaacgggga aagtattcgt
    39241 atttacaggt gattcaggtt atttggaaag tttcgctgat tttgcgaaag atgctgattt
    39301 gtttctagca gatacctatc tttttgaagg aaatgaacat catcatgccc atttcacttc
    39361 aaaagaagct ggagaacttg cgaaatcagc taacgtaaag aaacttgtac ttacacacct
    39421 acctcagttt ggtgatttag aacaattgaa aaatgaagcg caaaaggcag caggcaaagg
    39481 gattgatata gaacttgctg cagttgacaa agtgtttaac atttaggagg aagaacatga
    39541 tttttgtacc aaatgaaaac cacgacccaa gaatcaattt agcaattgaa caatatttgc
    39601 tgcaagaaat gcctttagat gaacctattt tgctctttta tatcaatgaa ccttcaatca
    39661 tcatcggcag aaaccaaaat acgcttgaag aaatcaatcg agattatgtg gaagaaaatg
    39721 gcattcatgt tgtccgtcgc ttaagtggag gcggagctgt ctaccatgat tttggaaacc
    39781 tgaactttag ttttatcatg cctgatgatg gggattcatt ccgtgacttt gcgaaagtaa
    39841 cacagccaat catccaagca cttcatgaat tgggtgttgc tggggcggaa ttgaaaggcc
    39901 gaaatgatct agtcattgat ggcatgaaat tttctggcaa cgcgatgtat gctaccaacg
    39961 gtcggatgtt tgctcatgga acaattatgt tcgactctga tatcaatgaa gtagtcaatg
    40021 cattaaaagt aaaaaaagac aaaatcgaat caaaaggaat caaatcgatc cgttctcgtg
    40081 taacgaatat caaaccattt cttccagaag aaaagcaaaa aatgacaaca gaagaatttc
    40141 gtcaagaaat cttgttgaaa attttcggcg tagagtcagt agaagaagta aaaacgtacg
    40201 aattgactga tgaagattgg aaaaaaatca atcgaatctc tgaggaatat tatcgaaatt
    40261 gggattggaa ttacgggaag tctccagatt tcaatttctc tcgccaaaaa cgtttttcta
    40321 tcggttcgat tgaagtccat ttgaatgtct cagaaggaat gatcaaagaa gcaaaaatct
    40381 ttggcgattt cttcggacta ggagatatct ctgacgtgga aaaaaaattg gttggacaga
    40441 aatatgataa agcatctcta tcaaaaatag tcagccaaat tgatgtgaag aaatattttg
    40501 gtgcgataga accagaagaa ttgcttacct tactttattg aaaataaaaa atagcggaat
    40561 ccacatatat ggattccgct att
//
LOCUS       DACDUU010000017        40232 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.017, whole genome shotgun sequence.
ACCESSION   DACDUU010000017 DACDUU010000000
VERSION     DACDUU010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.017"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            134..409
                     /locus_tag="IWL30_000934"
     CDS             134..409
                     /locus_tag="IWL30_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAP9705720.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            415..1182
                     /locus_tag="IWL30_000935"
     CDS             415..1182
                     /locus_tag="IWL30_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAP9705721.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            1350..3179
                     /gene="typA"
                     /locus_tag="IWL30_000936"
     CDS             1350..3179
                     /gene="typA"
                     /locus_tag="IWL30_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAP9705722.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            3440..5212
                     /gene="recQ"
                     /locus_tag="IWL30_000937"
     CDS             3440..5212
                     /gene="recQ"
                     /locus_tag="IWL30_000937"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAP9705723.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            5326..5628
                     /locus_tag="IWL30_000938"
     CDS             5326..5628
                     /locus_tag="IWL30_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAP9705724.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            5631..6794
                     /locus_tag="IWL30_000939"
     CDS             5631..6794
                     /locus_tag="IWL30_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP9705725.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            6820..10248
                     /locus_tag="IWL30_000940"
     CDS             6820..10248
                     /locus_tag="IWL30_000940"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAP9705726.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            10269..11393
                     /locus_tag="IWL30_000941"
     CDS             10269..11393
                     /locus_tag="IWL30_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705727.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            11383..11820
                     /locus_tag="IWL30_000942"
     CDS             11383..11820
                     /locus_tag="IWL30_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP9705728.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            11804..12142
                     /locus_tag="IWL30_000943"
     CDS             11804..12142
                     /locus_tag="IWL30_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAP9705729.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            12261..12821
                     /gene="rsmD"
                     /locus_tag="IWL30_000944"
     CDS             12261..12821
                     /gene="rsmD"
                     /locus_tag="IWL30_000944"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAP9705730.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            12818..13309
                     /gene="coaD"
                     /locus_tag="IWL30_000945"
     CDS             12818..13309
                     /gene="coaD"
                     /locus_tag="IWL30_000945"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAP9705731.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            13299..14342
                     /locus_tag="IWL30_000946"
     CDS             13299..14342
                     /locus_tag="IWL30_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP9705732.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            14501..15229
                     /locus_tag="IWL30_000947"
     CDS             14501..15229
                     /locus_tag="IWL30_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAP9705733.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            15345..15590
                     /locus_tag="IWL30_000948"
     CDS             15345..15590
                     /locus_tag="IWL30_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP9705734.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            15587..15805
                     /locus_tag="IWL30_000949"
     CDS             15587..15805
                     /locus_tag="IWL30_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP9705735.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            15941..16480
                     /locus_tag="IWL30_000950"
     CDS             15941..16480
                     /locus_tag="IWL30_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAP9705736.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            16473..18746
                     /locus_tag="IWL30_000951"
     CDS             16473..18746
                     /locus_tag="IWL30_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAP9705737.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            18784..19830
                     /gene="holA"
                     /locus_tag="IWL30_000952"
     CDS             18784..19830
                     /gene="holA"
                     /locus_tag="IWL30_000952"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAP9705738.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(19919..20173)
                     /locus_tag="IWL30_000953"
     CDS             complement(19919..20173)
                     /locus_tag="IWL30_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAP9705739.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(20573..20842)
                     /locus_tag="IWL30_000954"
     CDS             complement(20573..20842)
                     /locus_tag="IWL30_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705740.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(21312..21767)
                     /locus_tag="IWL30_000955"
     CDS             complement(21312..21767)
                     /locus_tag="IWL30_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAP9705741.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(21905..23833)
                     /locus_tag="IWL30_000956"
     CDS             complement(21905..23833)
                     /locus_tag="IWL30_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP9705742.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(24030..24863)
                     /locus_tag="IWL30_000957"
     CDS             complement(24030..24863)
                     /locus_tag="IWL30_000957"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP9705743.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(25055..25759)
                     /locus_tag="IWL30_000958"
     CDS             complement(25055..25759)
                     /locus_tag="IWL30_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9705744.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(25782..26408)
                     /locus_tag="IWL30_000959"
     CDS             complement(25782..26408)
                     /locus_tag="IWL30_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9705745.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            26734..29109
                     /locus_tag="IWL30_000960"
     CDS             26734..29109
                     /locus_tag="IWL30_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAP9705746.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(29133..29624)
                     /locus_tag="IWL30_000961"
     CDS             complement(29133..29624)
                     /locus_tag="IWL30_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAP9705747.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            29811..30731
                     /locus_tag="IWL30_000962"
     CDS             29811..30731
                     /locus_tag="IWL30_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9705748.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            30885..31229
                     /locus_tag="IWL30_000963"
     CDS             30885..31229
                     /locus_tag="IWL30_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAP9705749.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(31419..31919)
                     /locus_tag="IWL30_000964"
     CDS             complement(31419..31919)
                     /locus_tag="IWL30_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9705750.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            32447..32530
                     /locus_tag="IWL30_000965"
     tRNA            32447..32530
                     /locus_tag="IWL30_000965"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32481..32483,aa:Leu,seq:cag)
     gene            complement(32854..33492)
                     /locus_tag="IWL30_000966"
     CDS             complement(32854..33492)
                     /locus_tag="IWL30_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9705751.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            33827..34261
                     /locus_tag="IWL30_000967"
     CDS             33827..34261
                     /locus_tag="IWL30_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9705752.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            34284..34604
                     /locus_tag="IWL30_000968"
     CDS             34284..34604
                     /locus_tag="IWL30_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705753.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            34745..34936
                     /locus_tag="IWL30_000969"
     CDS             34745..34936
                     /locus_tag="IWL30_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705754.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(35007..35264)
                     /locus_tag="IWL30_000970"
     CDS             complement(35007..35264)
                     /locus_tag="IWL30_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAP9705755.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            35422..35685
                     /locus_tag="IWL30_000971"
     CDS             35422..35685
                     /locus_tag="IWL30_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705756.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            35709..35897
                     /locus_tag="IWL30_000972"
     CDS             35709..35897
                     /locus_tag="IWL30_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705757.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(35980..36165)
                     /locus_tag="IWL30_000973"
     CDS             complement(35980..36165)
                     /locus_tag="IWL30_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705758.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            36380..36751
                     /locus_tag="IWL30_000974"
     CDS             36380..36751
                     /locus_tag="IWL30_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9705759.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(36901..36973)
                     /locus_tag="IWL30_000975"
     tRNA            complement(36901..36973)
                     /locus_tag="IWL30_000975"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36938..36940),aa:Gln,seq:ctg)
     gene            complement(37019..37447)
                     /locus_tag="IWL30_000976"
     CDS             complement(37019..37447)
                     /locus_tag="IWL30_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705760.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(37507..38265)
                     /locus_tag="IWL30_000977"
     CDS             complement(37507..38265)
                     /locus_tag="IWL30_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAP9705761.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            38560..38757
                     /locus_tag="IWL30_000978"
     CDS             38560..38757
                     /locus_tag="IWL30_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705762.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            38863..39345
                     /locus_tag="IWL30_000979"
     CDS             38863..39345
                     /locus_tag="IWL30_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705763.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(39403..39999)
                     /locus_tag="IWL30_000980"
     CDS             complement(39403..39999)
                     /locus_tag="IWL30_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP9705764.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
BASE COUNT        13366 a         6835 c         8144 g        11887 t
ORIGIN      
        1 aaaaatcagt tgcgttttgt tttttagaat tatcaataat attattatgt aaacttaaaa
       61 atatgacaac ttcttggttt attaccactt cctatgctac actgaagagg aatatgaaat
      121 aggagagttg aagatgcaag aatatcagta tccacttgat ttagattgga caacagacga
      181 aatggtcatt gtaatgaaga tgtgggaaga tctggaacgt gcgaatgaaa aagggataaa
      241 caatgaagct tttttgaaga cttatcagca gtttaaaact gttgtcaaat ccattggtga
      301 ggaaaaacga ttgggacggg aatttgaaaa agcttccggt tattctttat atcgtacagt
      361 gcagcaagca aaaaaaaatc caaacaaaac tttgagaatg aacgggtgag ataaatggaa
      421 cagatgattg atcatatcaa gtcctggctt gaagaagctg gtgagaaaat caaatcttct
      481 caacaaacat taacaatctc gcaaaaatca aatcgaacgg atctagtaac aaatgtcgat
      541 cgaaacatcc agaattttct aatcgaaaaa atccattcct ttgatccaga ggcgaaaata
      601 ttggcagaag aagaagggtg taatcaatta tcggatctcg atggaagagt ctttatcatc
      661 gatcctattg atggcacctt gaattttgtg attgaagggg aaaatttttg tattatgctc
      721 gctgtttatg aatccggcgt tggaaagctt ggtttcattt atgacgtgat gagaaatgaa
      781 cttttttggg gtggaaaagg aataggcgtg tataaaaatg atgagccatt gcctagacca
      841 gcaaataaat ccttatcgga aggcttactt ggtgtgaata gttatctttt cggtcataat
      901 aaatatggaa tccgagaaat tgcggaaagc agcatgggga cacgtatgta cggatgcgct
      961 ggtcttgaac tagtggcaat cttgaaagga aaccacattg cttatatttc aaaccttagc
     1021 ccttgggatt atgcagcggg aaatgtctta ttggaagagt ttggtatggt aacaagcggt
     1081 ctttttggta ctcctttgac atttgaaaaa agggaatact atttagcagg gacttcttgt
     1141 gcatatgcag aaatcgcaga ccttttgaaa aaagaaggct gaaaaaaagg tagaaatcat
     1201 tcacaaaaat gataaaaacg agcaaaagat taattttctc gaaaaaatga aaacatttta
     1261 ctatatttca tgaaaaaagt ctgctataat tagcagtcga gtaaaatttg ccctgtcata
     1321 aagaaccaat aaaaaaggag ttagttacat tgaattatag aaacgatatc cgtaacgtcg
     1381 caattattgc ccacgttgac cacggaaaaa caacattagt tgatgaacta ttaaaacaat
     1441 cagatacatt agacgcacat acgcaattgc aagaacgtgc gatggactca aatgcgctag
     1501 aaaaagaacg tggtatcacg attttagcca aaaacacagc tgtagactat aaaggtattc
     1561 gcgtaaacat catggatact ccaggacacg cggacttcgg tggcgaagta gaacggatca
     1621 tgaaaatggt tgacggcgtt gtgttggtcg tagatgcgta tgaaggaaca atgcctcaaa
     1681 cacgttttgt attgaaaaaa gcactggaac aacatatcac accaatcgtt gtcgtaaaca
     1741 agattgacaa accttctgct cgtcctgagc atgtcgtaga tgaagttctt gaactattca
     1801 tcgaattagg agcagatgat gatcagttag actttcctgt gatttatgca tctgcactaa
     1861 atggtacgtc aagcttgtca gatgatccag cagatcaaga accaaccatg gcacctattt
     1921 ttgatacaat tattgaaaaa atccctgctc ctgtcgataa cagcgacgaa ccattacaat
     1981 tccaagtttc attacttgac tacaacgatt atgtaggtcg tatcggaatc ggacgtgtat
     2041 ttagaggaac aatcaaagta ggagaccaag tagcattaat taaattagac ggaacagtta
     2101 agaaattccg tgtaacgaaa ttatttggtt tctttggact aaaacgtcta gagattcaag
     2161 aagcaaaagc tggagacttg attgctgtct caggtatgga agatatcttt gtcggtgaaa
     2221 cagttactcc tgtggatcat caagatgcgt tacctatcct tcacattgac gaaccaacat
     2281 tgcaaatgac tttcttagtt aataattcac catttgccgg tcgagaaggt aaattcgtaa
     2341 ccgctcgtaa gattgaagaa cgtttgatgg cagaacttca aacagatgta tccttacgag
     2401 ttgaaccaac aaactctcca gatgcatgga cagtttctgg tcgtggagaa ttgcatttgt
     2461 ctatcttgat tgaaaacatg cgccgtgagg gatacgaatt gcaagtatct cgtccagaag
     2521 ttattgaaaa agaaatcgac ggtgtaaaat gcgaaccgtt tgaacgcgtg caaatcgata
     2581 caccagaaga atatatgggt agtgttatcg agtccttgag cttacgtaaa ggtgaaatgc
     2641 aagatatggt ccatacagga aacggacaaa tccgtttgac attcttaaca cctgcccgcg
     2701 gtttgattgg ttattcaact gaattccttt caatgacacg tggttacgga atcatgaacc
     2761 atacttttga ccaatatctg ccaatgcttc ctggtcaaat tggcggacgt caccaagggg
     2821 cgcttgtttc gatcgataca ggaaaagcaa cgacttattc tatcatgagt atcgaagaac
     2881 gcggtacagt cttcgtagaa ccaggtacag aagtatatga aggaatgatt atcggggaaa
     2941 acagccgtga taatgatttg actgtcaaca tcacgaaagc aaaacaaatg acaaacgtac
     3001 ggtctgctac taaagaccaa acatctgtca tcaaaaaacc aaaacaattg acgttggaag
     3061 aatctttgga attcttgaat gatgatgaat attgcgaagt aacaccagaa agcattcgct
     3121 tgagaaaaca aattttagag aaaaatgctc gtgaaaaagc atctaaaaag aaaaaataat
     3181 ctgtaaaagg atgaagagag aaaccagctt gttataaaaa atagctggtt tctttttttg
     3241 tccaactctt ttctgtggag aagtcatctt tgtcaatgga cataaattca gaaaatacgc
     3301 cgaaagacgt ttaaaagact tttaaaaaac ttatttctag agagtttttg tttcataata
     3361 agataatgtt tcacgaaagg agatcaactt gtccttcgaa gaaaatcgaa gtaaaatggc
     3421 aagagaaagg agcgggaaga tgaacgactt attaaaacaa tactttggct atgatgagtt
     3481 tagacctggt caaaaagaaa ttatccagaa agtcattgat caggaaaatg tattaggaat
     3541 catgcctacc ggaagtggga aatcaatctg ttaccagctt ccagctttac tattagatgg
     3601 gcttactgtg gttgtgtccc ctttaatctc tttgatgaag gatcaggtgg acgcggcgaa
     3661 tcaattaggt atacccgcca cttttatcaa tagctcgtta gatggttacg aaacagctag
     3721 acgcttccaa gaaatcgata gacagcagta ccgtttactt tatatcgctc ctgaacgttt
     3781 tatcatgccg gattttatcc aagcaatgaa gcgatggaac gtccgtatga tcgctattga
     3841 cgaggcacat tgtatctctc agtgggggca tgatttccgc ccaagttatt tacagatggc
     3901 gaaccagcta gatcaactgc caaatcgtcc tgtaattgtt gctttgacag ctacagcgac
     3961 tgtgcaagta gctgctgaca taaaaagact tttaaaaatt ccagaaaaca accatattca
     4021 gactggtttt gaacgagaaa atctacgatt tcaagtaatt aaagatcaaa aaaaagaaca
     4081 atatctaatc gaatatttaa agatcaacaa aaatcaatct ggtatcattt atgcagcaac
     4141 gagaaaagaa gtagaccgtc tctatcattt gcttaaaaaa ttcgattttt ccgtgggtag
     4201 atatcatggt ggtctaaatg agaatgagcg aacagaaatg caagaagcct ttttatacga
     4261 tcgcttgcag ctgattgtag cgacaaatgc atttggtatg ggtataaata aaagcaatgt
     4321 ccgttttgtt atccattatc aaattcctgg ctctctagaa gcatactatc aggaagctgg
     4381 tcgggcggga agagatggtc tttccagtga agccatcctg cttttctctc ctcaagatat
     4441 ccaagtccaa aagtttttta tccagcagtc ccaaagagaa gaagggcaaa agcaaaaaga
     4501 atatgaaaaa ttaaaagcga tgacagaata cgtgtatatt gagtcctgtc ttcagcaata
     4561 catcctaaac tattttggcg agacaagcag tccgtgcaac cgttgtggta actgtttaga
     4621 tgacagagaa atagtagaag taacgacaga agcacagatg gttctttctt gcttaaaacg
     4681 tatgggtgag aactatggta aacaaatgct gatgaaagtc ttagcaggat cgaaagaaca
     4741 aaagctgcaa gctttaggtt ttggacattt atctacctat ggactgatga aaaaccaatc
     4801 gcaaaaagaa acgatgcagt taattgaata tttgatatcg aatggctatc ttctgaccat
     4861 caatggtgag tatcctgttt tgaaagtaac agaaagaggg atacaagtat tgaaaggaca
     4921 agaatctgtt tatcgaaagg aacccaagaa agtccagcag ctatcggatg aagaaaccga
     4981 tacactattt gaagtgctgc gggaactgcg aacggatcta gcatcagaag caggcgttcc
     5041 cccgtatgtc gtattttcag attccacatt gaaagaaatg agccgaatac gtccatcaag
     5101 cagattggag atgcttcaga taaaaggagt tggccaaagc aagcttgata aatatggcga
     5161 agccttttta tctcggataa aaaatgcgga ctctaacgtt ctggataagt agaaagaacg
     5221 aatacgtaaa aatgaataaa aacaactggt taaatctgtt ttttttgctc cttatgaaaa
     5281 tcaaatatgt tataattaag gaattagaat gggaggtaag cacagatgga ttctttgtcg
     5341 aaaaaacttg cgatccaagt attaaatgat gaagcagaac ggatcaaacg attaatccga
     5401 aatcaaaaca acagtctctg tatctcacaa tgcaaagcct tcgaagaagt ggtagataca
     5461 cagatgtacg gcttttcccg gcaagtcggt tttgctgtcc gtataggagt aatcggaaag
     5521 aacgagggcc agctgatgtt aagtgagcta gaacgagaat tgaatcagtt atatagtgag
     5581 gtttaccaag aaatttatga taaaaaagaa gttggcaagg aggaatagac ttgcctaaga
     5641 aagtgaaaaa aaggcatcta ttagactata gcatcttgat tccctattta attttatgtg
     5701 tattgggatt gatcatggta tatagttcta cgtcgtatct attattagaa aacggacaaa
     5761 atccgtccgc atcagtcatt aaccagagta ttttttgggt gcttagcttg attgctatcg
     5821 cactacttta taaaatgaaa acagatgttt tgaaaaacca gcgtttgatc atggcagcca
     5881 tcgcagtttt gacgattttg ctgctgattg ttgtgttttt tggtaaggag atcaatgggg
     5941 cgaaaggttg gctgcaaatt gcaggattct cgatccaacc tgccgaatac ctaaaaatca
     6001 tttctatctg gtacctgtct ctgactttat ctaaaagaca aaacagtgta caaaaagatt
     6061 ttcttgggac tgtgaaacgt ccacttgcaa tggttatcgg acttactgca ttagtcgcta
     6121 ttcttcccga ttttggtaat gcggcagtta tctttttgat tattttggtt ctgcttttag
     6181 ccagcggagt caattatgtg tatactttga ttgtaggagt aggaggcttt tgtctaagta
     6241 cttttacgat ctggctgatc aatatcacaa acggaaagat cttacccgga cgtttacagt
     6301 atatctataa tcgatttgct atttaccaaa atcctttctc agacgaatta aataaagggc
     6361 atcaactagt caacggctac tatgccatgt tcaacggagg actatttggc aggggattag
     6421 gtaacagtat ccagaaaaaa ggatttttac aagaagcaca aaccgatttt atttatgcaa
     6481 tcgtagtgga agaactggga gtgattatgg ggattttgat attagcgttg ctattcttta
     6541 tgattgtgcg cattgtcctt gtaggtatcc gttcaaaaga tccgttcaat tcgcttttgt
     6601 gcattgggat cggcgcaatg tttttgatcc aagtatttgt caatcttgga ggaattactg
     6661 gcgttattcc tttgacaggg atcacgttcc catttttaag tcaaggaggc tccagtttgc
     6721 tgatgctttc gatttgtgtc ggctttgtcc tcaacatcag tgcggatgaa aaacgcaaga
     6781 gcttaggttt ataataaaaa aagatgtagg agtggttcga tgaaaaaagt tttagttgct
     6841 aacaggggtg aaatcgctgt acgtgtattt cgtgcttgta cagaattagg aattaaaact
     6901 gttggaatct atgcagaaga agatgaatat tccgtacacc gtttcaaagc ggatgaagca
     6961 tatttagtag gacaaggaaa aaaaccgatt gatgcgtatc tagatattga agggatcatt
     7021 tctatcgcga aagaatgcgg agctgacgct attcatccgg gttacggtct tttgtcggaa
     7081 aacttgaatt ttgcgcaacg gtgcgaagaa gaaggcatca tttttgttgg acccaaactg
     7141 catcatttag atatttttgg agataaaatc aaagcaaaag ctgcagctat cgaagcgggg
     7201 attgcttcga ttccaggcac agatggacca atcgcttcta tagacgatgc cttggagttt
     7261 gcgaaacaat acggttatcc gattatgatc aaggcagctc taggcggcgg cggacgcggc
     7321 atgcgtgtag ctcacgatga aaagagtgca agagagggct acgaacgtgc gaaaagcgaa
     7381 gcaaaagcag cctttggtag tgatgaagtg tatgttgaaa aatacatcgc taatcctaag
     7441 catatcgaag tacagattct cggggatacg catggaaacg tcatccatct ttttgaaaga
     7501 gattgttctg ttcagcgccg tcaccaaaaa gtagtagaag tagcgccttg tgtctctatg
     7561 aatgaacagc aacgtcaaaa aatctgccag gcagctgtcc aattgatgaa atatgtcggg
     7621 tatgtcaacg caggtacagt agaatttctt gttgaaggag atgattttta ctttatcgaa
     7681 gtcaaccctc gcgtccaagt ggaacatacg atcacagaaa tgattacaga tattgatatc
     7741 gtaacaacgc aactgttgat tgctcaaggt ttggatctgc ataaagaaat cggattgcct
     7801 cagcaagaag gaattaaatt aaatggttca gccattcaat gccgaatcac tacagaagac
     7861 ccgttgaata actttttacc agacacaggg aaaatcgata cgtatcgttc acctggtggt
     7921 tttggcgtac gtctggatgt cggaaatgct tacgcgggct atgtagtgac accgtatttt
     7981 gattcattgc tagtaaaagt atgtacgcat ggtgctacct ttgaaacagc cattcagaaa
     8041 atggaacgtt gcttgaaaga atttagaatc cgtggagtga aaacaaacat tccatttatg
     8101 ctcaatgtca tcacacatcc tgaattccaa tcaggaaatg caaaaacaac cttcattgat
     8161 agtacagcaa cattatttga atttcctcga ctacgagacc gcggaaacaa aacgatgaaa
     8221 tatatcggtg aaattactgt gaatggtttt ccaggtatcg aaagtggcga aaaaccattt
     8281 tatgaagaac cacgtatgcc taaagatcta atcacaagaa ctgattacgt aacggctaag
     8341 aatgtattag atgcgaaggg tgccgatgct ttggtcgaat ggatcaaagg acaggaaaat
     8401 ttgttgttga cggatacgac atttagagac gcccatcaaa gtctgctagc aactcgtgtt
     8461 cggacaaagg atttcaagca gattgcacgt ttgactggag aaggtcttcc tgaactcttt
     8521 tcaagtgaga tgtggggggg agcaacattt gatgtggctt accgcttttt gaatgaagat
     8581 ccttggcaac ggttgagaaa aattcgttct ttaatgccta atacgttgct gcaaatgctt
     8641 ttcagaggtt caaatgccgt tgggtattct aattatcctg acaatgtatt ggtggaattt
     8701 gtcaaagaag cagcagcgca aggaatcgat gtatttcgaa tctttgatag tttgaactgg
     8761 actccacaaa tggaaaaaag tatccaagct gtacgggata ctggtaaaat cgcagaggca
     8821 gctatttgct atactggcga tatcaacgat ccaagtcggg caaaatacaa tgttcagtat
     8881 tataaagaca tggcaaagga actagaacaa ctgggtgcgc atatcattgc aatcaaagat
     8941 atggctgggc tgcttaaacc gcaggcagct tatcgtttga ttagtgaatt gaaagaaacg
     9001 acagatctgc caatccatct gcatactcat gataccagcg ggaatggaat catcacttat
     9061 tctgctgctt caaaagcagg agtagacatc gttgacgtgg caatgagtgc aatgagtggg
     9121 aatactagtc agcctagcat gagcagcttg tattatgcac tggtcaatgg tccacggctg
     9181 ccagaaatca cgattgaaaa tgcacagaaa ttaaaccatt attgggaaga tgtgcgcatg
     9241 tattacaaac cgtttgaaaa cggcttgaat gctcctgaaa cagaagtata tatgcatgaa
     9301 atgccaggag gacagtattc gaatctacag caacaggcaa aagctgtcgg attaggtcat
     9361 cgctgggatg aaatcaaaca aatgtatcat actgtcaact tgatgtttgg cgatatcgtt
     9421 aaagtaactc catcatctaa agtagttgga gatatggcac tatttatggt ccaaaatgat
     9481 cttacagaag aagatattta tgaaaaaggc gaaacactaa gtttccctga atctgttgtg
     9541 actttcttcc aaggagaact agggcaaccc gttggcggat ttcctaaaaa attgcaaaag
     9601 attattctta aaggcagacc tgcattgaat gaacgtccag gtcttttggc tgaatcagtc
     9661 gacttcaacg aagtaaaaaa agaattggca gaaaaaattg gctatgaacc aaaacaagac
     9721 gaagtattaa gctatttgat gtatccgcaa gtctttttgg actatcaaaa agcatataat
     9781 caatttggcg atgttacatt actggacaca ccaacatttt tccaaggtat acgtttgggt
     9841 gaaacaatca atgtacaaat cgaacgcgga aaaacgctta tcattcgttt agatgaaatc
     9901 ggagaacccg atatcgaagg gaatcgcgta ctgttcttca atttgaatgg acaaagacgt
     9961 gaaatcacta tcaaagataa ttcgatcatc agtacggtcc aaacccgatt gaaagctgaa
    10021 ccgacaaacc gagaacaaat tggagcaacg atgtctggtt ctgttttgga tgtattagtg
    10081 aaaaaaggcg ataacgttaa aaaaggtgat acgttgatga ttactgaagc gatgaaaatg
    10141 gaaacggcca tcgaagcacg ttttgacgga gaagtcgcac atgtatatgt gtcttcagga
    10201 gacactattt cgtctggtga ccttttgatt gaagtgactg agaaataata agcgaaaggt
    10261 ggggaaacat gaaaagactg ggtttgtttt ggctgattct gctattagtt attgtggcat
    10321 tctacatgga accagtcttg tttccgccaa aacttgaagt gaaggaaaac tctgatcaac
    10381 aaatatcaaa ccaaatgaca acgtggaaat accaggagct taaagcaact ggtttttcta
    10441 cttatatcgg aaaaccgatc gatcagctgg aaaaggattt tggccaagcc tatgatcgat
    10501 taactagcgg atttggtttt gaaacaagat attatgaaaa taaagctagt catctcacat
    10561 ttgaagcgaa catccaagac agcagagtca caactgttaa agtactgcaa acagggcctg
    10621 ctgatatcgc gccgttttat ttagggatga cgatgcaaga tcttacgaaa atcactacta
    10681 tctatactaa tttcactttt gagtaccatg atacagaagt tggaatcgaa ctaatggaag
    10741 aggatatgaa ttatcgtccg ctaattgctt ttgataacga cacttttgct attttatttt
    10801 ttgatcaaac aacaaacggc ctattttcag ttgtgtattt agataaagat agtcttttga
    10861 aactattgcc atatcaagtg tttggtgaag gccttccaca ttatcaagca gataaatcgg
    10921 ctgattggga atcaatcaat cgcgttaaag aacgaaaaag tacgacttta ttaaatatgc
    10981 ttagaagaga agacgaactt cccatataca atcaaacact tgcctttcaa aaccacagtc
    11041 agctgctact acatgatttt ttgacaaggc ctgaggaaat attgtcagag gaacgtatgg
    11101 ctgattggca aaaatcgcta ggcagtgctc aaacgacagt gaaattctca ctgacgaatg
    11161 atgaattgga gaagttaatc agtaaacaaa aattagaaaa tactagtggg ctatttattc
    11221 atccggcgat tgacgctact ttttcgtttt tatattggta cagcgatcct tacaaccatg
    11281 accgtttcat ggacccggga acggattctg taggcattgc tttttctaaa gaaaatatgt
    11341 tagtcttatt gcaagaagaa aacggagaaa gtagtgatag cagtgatcgt taacgaagag
    11401 ctgtttcaac tggaggatca atgcctccaa gtcgctgaaa tgataaaaaa gagtaaaacg
    11461 atggcttctt atcttgaaca taaacagtca atggaagaat ctgcaaaagt ttgccagttg
    11521 aaagaagcgt tcttgtctaa gaaagaagca tttgaaaatg tagcggcata tggtgagtat
    11581 gcacctgatt atcgtgaaaa aaaacgagct gttcgaaaag caaaaagaca gcttgatatg
    11641 gatgagcatg ttgcagcttt ccgatatagt gaaacacagc tgcaaagtct gttggatcaa
    11701 attgtccaag tacttgccga agaagtttct aatgaaatca aagtagatgc aggtagcccg
    11761 ttttttgaaa cgaaaaaaca ctttggatgc ggaggtaatt gtcatgcaat tagacaatag
    11821 tgaatttgaa ataacaaaac gacgcgggct gatcgtatgg gtctacagct taaaacaatt
    11881 aaagaatctg aaaaagttcg gcttgttgca ttatgtctca agaagaatga aatatgtcgt
    11941 gctttactta aatgaagaga cattggaaga aacagaagaa cgaatccgta aattacattt
    12001 tgttcgaaac gtagaacgtt cttaccgacc ggatatcgaa atgaattttg ccgaaaaaat
    12061 aggaaacaaa acagtattaa aagacgaagg tttcgaaatt gaagagctaa atacagaaat
    12121 ccgattagct gaacatcttt aaaaatctct tatgagcagt ttttaaataa aaaataactt
    12181 ggataacgat cgatttttga tcattgtcca agtatttttt tggtatacta gtaccagtac
    12241 catttctaag gagtgagtag atgcggataa tatcaggaaa ttatggcggg cgccgtttaa
    12301 aaagtttagc aggggcgaac accagaccaa ctacagataa agtaaaagaa tccatattca
    12361 atatgattgg accatatttt gatggagaga cagtattgga tctgtttgca ggaagcggtg
    12421 gtttagctat cgaagcagta tctagaggct gcagtcatgc ggtatgcgta gacaaaaatt
    12481 accaagcgct aaaaatcatc aaagaaaaca tcgaaattac gaaagaacca gagaaattta
    12541 ccacaataaa acttgatgcg gacaaagcaa tcgaacggct agcagcggag aaacaacaat
    12601 tcgactactt gtttcttgat ccgccatatg caaaacaaaa aataatcgat caaatccata
    12661 aaatggaaga actgggactt ttttctaaaa ccgcagttat cgtatgcgaa acggataaaa
    12721 cagtcgaatt gccagaaact gtggctggat tccatcaaat aagaaaacag atttatggca
    12781 taacggccgt aactatctat agaaaagagg gaaatcaatg aaacgtgcat tattcccagg
    12841 aagttttgat ccttttacaa aaggtcactt agatacggtg gaacgtgcgg ctaaattatt
    12901 cgatgaggta gtaattggtg tttttatcaa tacttcaaag aaaagtttgt ttcccccaga
    12961 agaaagaatg acattgatca caaaagctgt ttctcatctt cctaatgtaa aagtcatgca
    13021 tcaagaaaat caattgacgg tagaaacagc aaaagagatt ggtgcagatg cgttgatcag
    13081 aggcatacga agtatcaaag actttgaata tgaacgagag attgctcaga tgaatcatca
    13141 tctatatgat gaattggaaa ctgtcttctt attagcaaaa ccagaatata gtcacgtgag
    13201 ttccagtatt ttaaaagaag tactgcactt tggcggagat gtgagcagct acttaccgcc
    13261 agtgatcaac gaagctttag ctggaaagcg tgaatcaaat gaaagctaaa caatggttga
    13321 aaacagtact gattatcata tttggaatgt tgatcattgg atttgtagta gttcccttgc
    13381 cgtattatgt agaatcaccg ggagccacga tcgatctgaa agaactaatt acggtcaatg
    13441 ataaggaaga taaaggttct gggtctttct ctcttacctc tgttggtatc aggcaagcta
    13501 caggtttctt agctttaaaa gctaaaatga ccccttttga agaattgatc agtgaagaag
    13561 aattgactgg tggggcgaca aatgaagaat ataatcaaat gcaacagtat tatatggatt
    13621 cttcccaaaa tgcagcaatc gaacaagcgt taaaacttgc agacaagcct tataaaatgg
    13681 aattcaaagg cgtatatgtc atgagtgtag agaaaacatc gaatttcttt ggcaagcttt
    13741 cagttggaga tacagtaaca aaagtagacg ggaaaagctt tcaaagtaca gaagcattta
    13801 tggattatgt aaaaagccaa aaagttggac aaacaatcga aatcagttat ttgcataatg
    13861 gtgaagaaaa aacagcttct ggtgatctga tcgagttacc aacagataaa aaggcaggaa
    13921 taggtatcac gcttactgac cataccgaga tcgaatcaga tacagatgtt cgtattaatt
    13981 ctggctctat cggtgggcca tcagcgggat tgatgttcac tttagaaatc tatgaacaag
    14041 tcactggtaa aaacttgcga catggtaagc aaattgcagg aactggaacg atcaacagtg
    14101 aaggagaagt tggccgaatc ggaggaatcg ataaaaaagt ggcaagtgcg gataaagctg
    14161 gcgtagagat tttctttgca ccagatgata cgatttcaga cgatgttaag aaagaatatc
    14221 ctgaaataaa aaccaactac gaagaagcaa aagctgccgc taaaaaattg aattcctcaa
    14281 tgaaaatcgt accagtaaaa acagtacaag aagcgcttga ctatctagag aaaatgaaat
    14341 gaaactaaat aattaaagag gggttgtgac acgagttctg tcacaacccc tctttttcgt
    14401 caaaaatctt cacgataaaa ataaaaacaa aagaaaaaat tgacgtaatt agatgttgaa
    14461 gggacgattt tgccagagct cactgtttct tcaattttaa atgaaaggag taatgaatga
    14521 tttttactgg tcattctttg aaaaaagaac aatgatagaa aaaatcagca agaaaatact
    14581 agcttcaccc aaattgatca tttggatgtc tatgatagtt cttatctttt gtatcgcaag
    14641 tggtttttta ttatcaaatt tgctatttta tagagaaaca gaagagactg ctgcaaaaga
    14701 aatactttct tcgtttagtt ccagtggaga agatccatca gctgaaggaa acgatgtaga
    14761 agaggatgct cgtccaaagg tcatgtacac cgatatcaaa ggaagtgtga aggagcctgg
    14821 tatctattcc ttttcttcag aagaacgggt ctatgatgta ctaaagcgtg caggtggatt
    14881 attagaagaa gcggatagcg atcgaatcaa cttttctgcg aagatagaag atcagcaagt
    14941 cctatatatc cctgctgtag gtgaagagct gccagaacat cttgatcagt tggcatcctc
    15001 ggaaggaaaa cagcctacag cagattccga gcctcccaaa ataaatatca atacagccac
    15061 tccatcagaa cttcagcaga ttcctgggat cggatcagtc aaagcgcaag aaatcattcg
    15121 atttagagaa gaaaacggat ccttccagaa agtggaagac ctgcaggaga tttctggtat
    15181 cggtgaaaag acagttgaaa aactaaaaaa cttcgttaca attaaataga atgattcaaa
    15241 atcgactggc acgagcaata aatggacgct aatggatact aagaataatc caattctaag
    15301 agcttggcag gacgaggagg tagtattttt ggataggaaa tagcttgatt gttcgcaaag
    15361 aaacattaaa aaaaccaatg ctgaatgtct atctccaaaa taaaatatct gggattcata
    15421 ttatgaatac ggctgtcagc ggcaataaca gtcaagctct acgagaacgc tttgccaaag
    15481 atgtacttag ctatacagca gataaagtat ttattctcat tgggacgaat gatctagcag
    15541 agcataaaca actttctaaa gaaacgtacc aaaaaatctg cagtggatga tagttcagct
    15601 gaaaaaagac tatagttctg atcatgcttt ctttatcacg ccaccagcag tcgatgagac
    15661 aaaacaaaaa tatcggaata acaagttgat caaagaatat atctctgttc tcacagaagt
    15721 agcaaaacgc catgattgtg aagtatcgat ttttatacgg aatttcttga acaaatggat
    15781 tcgaagaatt tttataatgg gctaatggat gacggacttc atttcggcag tgaagggtag
    15841 gatcttttgt cagacaggat gattcgttat atttaaagaa tattttgttt aagcaatgag
    15901 tactttttaa tcaacttgaa aaaacggagg aatataaaac atgttagaaa aatcttcaaa
    15961 agcatgcgat acgaataaag aaaaagttac tgataagtca cataatgaac gcattccttg
    16021 ggaccagtac tttatggctc aggcagtact gttatcttta cgtagtacgt gtacacgttt
    16081 agaagtagga gcgacactcg ttcgagaaaa aaggatcatt gctggcggct ataatggagc
    16141 agtcagtgga gatgtccact gcatagatga agggtgttac gtcgtggatg gacactgctt
    16201 gcgaacgatc catgcagaga tgaatgcact cctacagtgt gcaaaacttg gtatcccgac
    16261 ggaaggttca gaaatctatg tcacacattt tccatgtttg gcctgtacaa aggctttgtt
    16321 gcaagctggt gtgaaaggga tcaattatct ccatgactat cgaaatgatc cttatgccca
    16381 agcgttgatc gaacaattag gtgtatctgt acaccaagtt gctttagact cacagcattt
    16441 ttcaaaactt caaaatgaat taacaggaaa acatgtctag cgttttagaa aggcatgttc
    16501 gaaatcggtt gatttttccg gcaattacgt cgatgattat ctgtttgttg tattacactg
    16561 acaattgggg ttatcgtttg cttcttgccg gaattttttt atggttttcg ctaaaagaag
    16621 gacgttttat cgcgtacttg tgtattttgt gtagcataac ggtatccatt tcttataggc
    16681 tacaagagta ttcattttta gaggaaaaaa caacatctgc gcaaattact gtatttacag
    16741 atacgattcg tgcaaatggt aattttttga caatggaagg aaggttatca gagaataaac
    16801 aaaagctcca gctttcttat caagtaaaca cagaagcaga attgaaaaca tggcttaact
    16861 tccgaggaaa gggaatagaa ttgactgctg aaggcgagtt catctctcca gcaaaaaagc
    16921 ggaatcttta taattttgac caagaagctt acttcaggaa ccatagaatt tctggacggt
    16981 tccaaattca aaagattgaa cagatcacca tgcaaaatca ctggcgaaac tggcttcaga
    17041 gaaaaagagg atcttttatc tcctttgtcc aatctgaatt tccgcaaaaa acaagtgtat
    17101 atattaattc tttattatgg ggctataaag atgcaggatt cagagaacca gaagaaattt
    17161 ttagggagag tggtttgctg catctgttca gtctatcggg tctacacatc caattttatt
    17221 tagggtggct gtattattta tttcgacgta tcggatggcc actacacgtc agtattattc
    17281 cgttgagtct actaactatc tgttatgttt ctattgcagg aagttctatt tctgttttgc
    17341 gtgctagttt gctgttttta ttgcgattgc tagtcaaatt gctgaataag cattattcca
    17401 ccatggactt atttgcagtt gtcttttggc ttcttttact atttgatcct cgatttcttt
    17461 ttcatgcggg tggacagctt agttttttca tgagtttttt gtttatcctg atttcttttg
    17521 atggagattg gttgaaaaaa acggcctctc acgtcctgtt tactatgatc acgatgccgt
    17581 tgattgtatg gcatttttat gagtggccta ttttaggtag tctttttaca ttactgattg
    17641 caccggtatt caagtttctt tttcttcctg gtgtgttatt tctcacactc ttcaatcgct
    17701 ggctgccgga aactttactt ttgctgatag aagagggatt aatcttattt gagcgaataa
    17761 tcgatttttc ttcagggtcg tctctcatta taggacgttt gccccttgtg ctatctggga
    17821 tgtatatttt cggattactg atttgtatgg ataggttgaa agaacaacct gtgaaacact
    17881 tgttttatgt tggtcttttt tcttttattt ttttgacttt gccattttta cgcttttctt
    17941 caacgatcgc ttttatcgat gtcggtcaag gagacagtat ctttttgcaa agtccttttc
    18001 gaaaagaaac gattttgatc gatactggag gtcagttaca ctttgaaaga gaaaagtggg
    18061 cgcaaggtat gatacgtcct cctgctgaaa gaaatatcgt tccatatcta aaaggtcagg
    18121 ggataagtgt aatcgataaa ctcatcttga cacacgacga tacggatcat gtgggagaat
    18181 tacccacgtt atctcagcat tttactatca aaaaaattta tatcggttgg ggcgctggac
    18241 gaaatgagcc attacaaagg catttatctg aagctcaaaa aaatggtact gagatcattg
    18301 aaatcaaaca tggggacagg atcagcggtt attatgatct ttatgtattg actccttttg
    18361 aaaagggaga gggaagaaat gaagattcga ttggtctatg gatggaatat aacaataggc
    18421 gctttttatt tttaggagat ttgaatcaag caatggaaaa acaattgctt ttgatttatc
    18481 ctaatctgaa agccgatgta gtcaaattag gtcatcacgg tagccgtacg tcaagtaaca
    18541 cagattttct ttcccatatt gaagcaaaac atgggattat atcctgcggc gtaaataatc
    18601 gttatggtca cccgcacagc gaagtattgg agacactaga agaaaatcag ataaaaacat
    18661 ggcgaacgga tcaacaagga atgatcacct atcgctggca tccagttttt catcctcatg
    18721 ggctagtgga aacaatgata gactagtaat tctcatctgc tcatggtagg ataatttttg
    18781 aaagtggtga gtaatttgag tatacaagca gagttacaaa aaatacgtaa agaaaaactt
    18841 gcatcttgtt acttgatttt aggaacagaa aagtttctcc aagatcaagt tcggacagaa
    18901 attgtcaaaa aaatccaatt agagggcgaa gatgacttaa attttttaag ttttgacatg
    18961 gaaaatgcaa gtttagatga ggtagtagct gaagcagaga cgttgccgtt tttcggagat
    19021 caacggttag tattcgtaga gaatccttat tttttaacag gtgaaaaagt aaacaatgga
    19081 atcgaacaaa atactgattt attgacggct tatttgaaag aaccattaga atcgactgta
    19141 ttagtattct ttgcgccata tgaaaagttg gatgaacgga aaaaagtaac gaaacaattg
    19201 aaaaaaacag caattatcat caatgtccaa cagttgaatg aaaaagaagt acgtcaatat
    19261 ctgatgaata ccttagagaa ttcctcaatc aaaatggaac gaagtgcaat tgatttattc
    19321 ttgaggttga ccgacttaga tttatccaaa atgatgagag agttacagaa attggtcctt
    19381 tatggacaaa atcaacagca aatcacggta agagaagtag aagaattagt tcctaaaacg
    19441 ttagaacata atatttttga catgacccaa tatattttaa aagggaagac ggaacaagct
    19501 ttgcgtttat acgaagattt agttatgcaa ggggaagaaa caatcaaaat taatgcgatt
    19561 ttgctttctc aattacgttt atttttacag acaaaatttc ttgtaaagat tggttatcag
    19621 caggcaaata ttgctgaaac attgaaaatc catccatatc gtgtaaaact tgccatgcag
    19681 gaagtaaaaa aattcgatga acatttgctg gtacagttgt ttgatcaatt agttgagatg
    19741 gattatcaaa tcaaaacggg acaaatcgaa aaagaattaa gttttcaact attcgttttg
    19801 cgcacagggc agttgttaag aaatcgataa gaagaaagac gagggcaaag catgcgggaa
    19861 ggtcgtttgt ttttgtcctt tttttgacaa ataaaaaaat cggccgaagc cgattctttt
    19921 atttagcgat ttttttgctt aaacgagatt tgtcgcggtt agctttgttt ttgtggatta
    19981 gaccttttga ttcagccata tcaatagctt taacagcttc tttatataac gcatctacgt
    20041 tgtcagcacc tgaagctaca gcatcttcaa attttttgat tgcagtacgc atagcgcttg
    20101 tttgagatga atttttcaca ttagcgtttt cgcttgtacg aacacgtttg attgcagatt
    20161 caatgtttgg cattagtttc acctccgata acttaagttc ttacgcgtgt taccgcataa
    20221 ttcaacattc attattatac ctaaaggcag cgtgctttgc aatatgaaca tgacaagttt
    20281 ttttccttaa cagtctttta ttaggatttt ttttcagtaa aaacggatag tacaagctga
    20341 aaaaaatcaa aaagacgcta ttgtgggagg caatagcgtc aaaccaaact agatttttat
    20401 ttatctattt aaacaaagtt tgaatgggaa tgtttggtac taagttatta tataacaaag
    20461 agaaccgttt gcaaagtttt ttttgaaaag ggatacgatt ccctgttgtt ctttgttttt
    20521 ctattaagaa actgcagtta ttttataacc agtatccttt gatcagtaaa ggctattctg
    20581 gtgtaccgat tattttaatg tattcatcga tattttttat caacattttt tttatgacca
    20641 actggttccg acggttccat ttgccactta cttccagcac atatttattt aggggcaaac
    20701 gtaagatata ttttgccaaa tagcgatctt taagcaggcc attgatcaca cggtcctcaa
    20761 gctgcaagtt gatcccagtc agcatcattg gctgttcttg taaaacgtac gtatcaatta
    20821 aaagaccttc aaaagtttcc acaagcgtct tcctttcttt tcaaagtaac ctgaaaacag
    20881 gcactaattc ttccattttg atattcgttg aagtggatgc gatcattctt tcttttgtcc
    20941 actgtaatct agtataacaa aaaagagcag aagagccaaa aagaaaaaag agcatcgaat
    21001 tctataggaa gtctcattgg cacggcctaa attattacaa aacattgatt aaaaaaagcc
    21061 cctgcatcgc aggagcatcc cctatatcgt tttaataggt caatcaaaaa gcagtatcaa
    21121 acacattttg tatcaaatca ggaaataata gataagctaa gcccagaatc aaaagtaccg
    21181 ctagaagaaa ccagccgatt gttcctatga aatactttaa tttttcgaac attgtatcac
    21241 ctcgttgtca gtatatcacg gatcagtaaa ttgggggtgt taagaatctc ctaaaataga
    21301 caaaatttgt tctattttcc taaagcaaat acttgtaaga tacagtaagt gataccagaa
    21361 gcaatgaccg gtccggcggc gattcctctt aagaagacaa cacctaaaat cgtgccgaaa
    21421 acaagtgcaa cagtgatttg tggatcagaa gcaatcaatc ctacaccctt ggaagataag
    21481 acagccacta gcccaccgca aaaaacagct acccagccaa taggggattt aaaggcttct
    21541 aatagatcac gtagaccgat ttctcctgtt gcgataggga ttagaatagc cgctgtgatg
    21601 atcgtcaccc caatattgat tcctttgcct tgaagccaag ggaaaaagga acgggaggct
    21661 ggtatgagct tcataatcaa aacaaaagct gccgctatca tcagactttg atttttagct
    21721 aaaattgcta caagtaaaat aattcctaaa aaaagccaac tttccatgaa atatattcct
    21781 cactttcttc gaaacaatca taacatgttt ttagaaagtt acaaaagtag gaattcatcc
    21841 ttcaagacct attcataagc tgtttttttc ataaaataaa caaaatcata aaaaaacact
    21901 ttgcttattt gacagaagac agccgcgctt tgtatagtgg acaagactta catttttttg
    21961 aagaatcaga aagatcaggt aaaggtctta gtaaacgcaa gccattcaga ataaccaata
    22021 ttgtgcttcc ttcgtgtcct acaactccaa gaggtagatt taaaaattgc aggaagttag
    22081 aagcaatcaa caagagaata acaagaatcg aaaaaataat attttgtttg atgatccgcc
    22141 gcatttttaa agatagccga tgactgtaga caaatttatt taagtcgctt ttcattaaga
    22201 cgatgtctgc gatatccata gcggcatctg tcccttgccc catagcgaca cctaaagaag
    22261 cggctgctaa agcaggcgca tcattgactc catcaccaac catcgcattt acttggtaaa
    22321 gttcattctc ttttttgact agttgtgttt tttcttccgg agtacagctt gcatagtaat
    22381 gttcaatccc tacttgttca gcaatggcct ttgctgtgcc agaatggtcg ccggttatca
    22441 tacttgtatg gatgttttgg gatttaaagt aggaaattac ttgttgggta tttgcttttg
    22501 gaatatctag cagacctaaa acagcaatta actgctgatc tttagaaaga taaatcacgg
    22561 ttttgccttg attttccaaa cgttcgatca tttccgcaat ctctttgctg attatcatcg
    22621 tctcaggatc atatgcgtgc ttgccaactt tccattgcgc acctaaatag gtagtttgta
    22681 atccaaaacc agtaatttct tctattggta agtattggat ttcttcaggt attgtgcaat
    22741 cgaaatattg caaaatcgca tgagcaagtg gatgcgtcgt tttttgttcc atagcaacaa
    22801 gaagttgttg tgccaaatcc ttttcatcaa agaaaaagct gtcagttaca actggttttc
    22861 ctttagtgag cgttcctgtt ttatcaaagg caatcgcttt taattcagaa agcttttcta
    22921 aatggatacc gcctttgatt aaaatcccat tttttgcaga atttgaaaga gcagctagag
    22981 aagctggtgt agcagatgcg acaagcgcac aaggagaagc cacaactaat aataccattc
    23041 ctcgataaaa gctttcatcc caactccaac ctaggaaaaa gtgggggaga aggatcatta
    23101 gaggaacagc aagcaaaatc aattttacat aattattttc gattcgttcg ataaatgaag
    23161 ctgtttgagt tggcgtattc tgcgcttctt ctactaattg gataatctta gcaaagacag
    23221 tttcggaact cgtttttgtt actcgaatcg tgatgggatt tccttgattc agcgttcctg
    23281 catacatctc atcgcctaaa tatttttcta caggcacaga ttcaccgctt aatgcagact
    23341 catcgatcgt tgcctgtgtg ctttctaaaa agccatctat cggaacagct gctcctttag
    23401 cgacaaatac caagttatcc acctttagtt gagaaacatc tacttcttgc atttgtccat
    23461 tttcttttaa taattgggct ttttgtggtt gaagattcat caaagcagta atttcttttt
    23521 gacttttatt agttgtgtat tcctctaatg caccgcttaa acaaaagatg aatgtcaaca
    23581 ttgctccttc aaagtaatta ccgatcaaac aggcaccaat agcagctaaa gccatcaata
    23641 aatccacatt cagtgtccga tcattccaca attctttcaa gccttccgat gtttgtttat
    23701 atccaccgat gataatggca ctgatataaa aaagtggggt gatggtaaga gagaatcggt
    23761 cgaagataaa accgaagatc ataaataaaa ggcagccgat tgttgctgca aaagcttttt
    23821 tctgattagt catcgttatt agacctcctt gtcaaaacaa ggatagcata ataaaaatgt
    23881 aattgataat aataacagct aaatgagaac gataatggtt atcatttggt gattaagctt
    23941 gatgttgttt ttattattta taatgattat tattaaagaa aataaaaaac cagaacattt
    24001 ctcaaataag agaagtgttc tggttttttc tacattgcaa taaaccaata accaatcaaa
    24061 atgataccaa ggataatacg ataccagcca aaagcggtaa agtcgttttt cttcaaatag
    24121 cttagtaaga atttgatagc taagacggaa accacaaaag atacgaatgt tcctacaaga
    24181 agaacaacag tttcttccat tttgaatgaa ttcccttcag ctaaatattt gacgatcttc
    24241 aagccgcttg cgccaaacat gacaggaatc cctaagaaaa atgaaaactc tgtagcgaca
    24301 aaacgcgaag caccaatcaa gatagatcct aggatcgttg cgccagaacg ggatgttccg
    24361 ggaacaagtg ccaagacttg gaataaccca acaagaagag ctgcttggta cgtaaagtca
    24421 ttcagagaag tccaacgagg tgttttgttt ttgttgcgtt tttcgacaat aataaaggca
    24481 ataccgtaaa cgatcaaaac aatggcaact ggtaagaact tgtggaaatt tgcttctaac
    24541 caatcatcca aaggcaaacc aatgatgatg gctggtatgc aagcaacgat cactttaaac
    24601 caaagtgtcc acgtatcttt tttttctacc gttgatttac ttggagaaaa tgggtttaac
    24661 ttgtgaaaat aaagaacgac gacagccaag atcgccccta attggataac gacattgaac
    24721 atcgacataa attgtgaact ggcatccagt ttgatgaatt catctgctag gatcaaatga
    24781 ccagtactac tgatgggcag ccattccgtt attccttcaa taataccgag aatcactgct
    24841 ttgataatat ttgcaaaaaa cataaattcc ttccttccca attaagatac atcttatagt
    24901 ataccgttat tttttgagaa acccaatctg ttttttaata ttaagaaaaa cttgagaaat
    24961 aaagcagata agtacacgaa gagaaaaaag aagacaaagc cctcgttcag actttgcctt
    25021 ttatgattct ctggtaaaaa ttaaaatatc cccattatag aaacgagtcg cgactatgtg
    25081 ttttcccgtt atcgatataa gacgtatcat tgatgccgtg gatagaaaat cgaccgtttg
    25141 cataagtgac ctttgttacg ctgccattta aaagcccagc acgaacaggc tgtttaggat
    25201 cgatcagatt aagcaaaaag gcgatcgtca aaccatgaga tacgactaaa acatttccac
    25261 caccattttt ttcgcagtga tgggcgatat cttcaaatcc tgtccataca cggtctcgca
    25321 atgtttcata agtttccgcc cacccagcgg tatctaagtt gtatacagca ttagcaattt
    25381 gttcaaaaga aacttctgtg gtaaacattt catcatatgt tttaaaattc aatacacgag
    25441 ggatcacacc ccacatttcc atgtcatatc caccttcaag agacccgaaa caccattctc
    25501 taatcctttt ttcaataaaa taaggaattt cttttccctt agaatgttct cctagaataa
    25561 tacgggcagt ttcgattgca cgaccactgt cactagaata agcaaatgaa aagtcgacat
    25621 tttttaaccc gcgacctaaa taatgaatgc cttcttgtcc ttgctttgtg aggggtgtat
    25681 cacaccagcc ttgtactcgt tcaattgtat tgaacatcgt ttttccgtgt cgtacgatat
    25741 aaagtgtagt ttctgtcatt ttattctcct cctaagtcgt tttaacgatt gaaaaaagga
    25801 tttgtccttt tttcctgttc aatcgtcgtt gcatcgccat gtcctggata gactgcaaat
    25861 tcatcaggta atacaaacag ctgggtccga atactatgaa gtaattgttc taaattccct
    25921 gtatacagat cggttctacc gatacttcct ctaaacaggg cgtcgccaga aacgacaaaa
    25981 tcatcaaaaa taaagctgac gcttcctata gaatgccctg gggtagggac gacgcgaaaa
    26041 gacatgtttc ctaaagtata agttttcatt tcaaattcat attctgcagg cgagacaata
    26101 acatcagcaa tatcatcatg tctaccgagt ccagaaagat tcaaaatcgg atcacctagc
    26161 catttttgct ctagtgggct gacataaact gggatttgat aatacgcgcg taaaggttcc
    26221 actgcaccga tatgatcgta gtgagtatgg gtaagcaaaa tcgctaccgg ctgttggttt
    26281 gtttttttga tttcttgttt gattcgttcc gcatcttctc cagggtcaat gatcaaaagt
    26341 gcttcttcgt tgtagaccaa ataacagttt tcttcaatca ttcctgtttt taatctttcg
    26401 atatgcatgt gcattcctcc ttgatagcca aaacttcttt catccattag tataacgcat
    26461 tcattgctag gaaacaaaaa caggtactta tttctcttta cttatctgaa ttatcagact
    26521 atatgaaaag tatttggata ttctaaagag aaggaaagta agttttcata gcattttaat
    26581 atgtccttgt tacattagat tcatccaaaa atccaaattc tatatcctat atccctgcct
    26641 aatcagcagg gtgttttttt atgtttggaa agagagctct ttttgctaaa ctaggttttt
    26701 tcaggtaatc ttagagagaa agaggtggtg gaaatgatca aacagtcaaa aatatctgtg
    26761 cggcacttag tagaatttat tttgcgacgt ggaagtatcg acaatcgtaa aaaaagtaac
    26821 catactgcat tagaaggagc gagaatccac cgcaagctcc aaaaagatgc gggagaaaac
    26881 tatgaaaaag aagtcttttt aaaaacaaca gttcaattag atgaatatta cctgatcgtt
    26941 gaaggccgtg cagacggtat tttcaaaaga gacggtattt attatataga tgaaatcaaa
    27001 acctccgaac cacgatttga agatttagaa ccagaacaag tggaactttt ctttcatcaa
    27061 gcaagggtgt atgcctatat ctatagtcaa gaacatgatt tacaagaaat aaatctgcaa
    27121 ttgacttact tccagacggc agaagaagtg atcacgagaa aggttgaaca tcagacaaga
    27181 gagcagttag aaactttttt taaaaagctg actgaagatt ataaagaatg gcttgttttt
    27241 caagaaaact ggcgcactgt acgcaatgcc tctttgatgg ctttgaaatt cccccatgat
    27301 gagtatagga aaggtcagcg tgaactagcg gtagctgctt ataaaacgat ccgtacgaaa
    27361 cagaaattgt ttgtagaagc acctaccgga acaggaaaaa caatctctac gttattcccc
    27421 gctttgaaag ctgtagggga agaagaggga gaaaagatct tttatctaac ggctaaaaca
    27481 ataactagac aagtagctga agatgctatg acagcattaa aagatacagg agctgaagta
    27541 aaaagtgtca ctttgacggc gaaagacaag atctgctttt tagatgagac gacctgtaat
    27601 ccagatcagt gcccatatgc caatggttat tacaatcgga ttaatgaagg tttatgggat
    27661 cttttgaatc atgagaatca gatcactcgt gaagtcatcg aaacttatgc aagaaagcat
    27721 acactttgtc ctttcgaact atcattagat gtcagtatct ggtgtgacgt aatcattggg
    27781 gattacaatt atttatttga tccaactgtt tatttaagac gtttttttga agatgaaaaa
    27841 aacgaggact atctgttttt gattgacgaa gcacataatt tggtgaatcg ttcccgagag
    27901 atgtattcgg cagaactatc ttatgaaaaa acgaaacgaa gtaaggaagc tataccaaag
    27961 gaattcaaaa aacttcatcg ccgtttcaac aaactattaa aagaatttga ttcgatccga
    28021 gaaatagcaa aagaagatca ttgggattat catcatcaaa aagcaccggc tgaatcttta
    28081 gtaaaggcag gttatcaatt aagcgaaaaa attaaagaat ggctggcaga atttcctgag
    28141 cacccatcac aagaacaatt gcttccttat tattttgatt tgcttcattt tttgaaggtc
    28201 agtgaatttt atgatgatca ctacgaaaca acagtcgaaa aaacgtatcg cgatctgata
    28261 gtaaaagagt tttgtattga tccaagcttg tttcttgagc agtcgttgga taaaggaaaa
    28321 agcagtattc ttttttctgc tagtttttca ccactctctt attatcagga aacactgggg
    28381 ggacaaaaaa gtctggctta caaactgccg agtcctttcc cagaaggaaa ccagcaagtt
    28441 ttcatcgcaa attacctaga gacgacttat cgaaaaaggg aacacagtct ggcaaaatta
    28501 gtagagacga ttcatgcttt tgctgatgga aaggtcggga attatttcgt tttctttcct
    28561 tcttatcaat atttagatct tgcggtggaa gcattcagac agcaatatcc cgacagcaat
    28621 gtgctaatcc aagaaacgga catgaatgag gaagaacgcg aacgattttt aacaaaattt
    28681 caaacaaatc ccgctgaaac attgattgga ttttgcgtac ttggcgggat tttttcagaa
    28741 ggaatcgatt tgagagggga ccgattgatc ggcagtatga tcgtaggtgt aggtttgccg
    28801 cagatgaatc atgagcagga actgatcaaa tcatattatg atgagaagga acaccttgga
    28861 tttgcttacg cttatcagct tcctggaatg aacaaagtct tgcaagctgg tggtcgagtg
    28921 attcgtggca tggcggatca aggaatcgtt gtattggcag atcaacgatt ttctagtttt
    28981 tcttatcgac gtttattccc acaacattgg caaacagcaa gagaagtacg aagtgttggg
    29041 gaattggaag aaggaataca gcgtttttgg ttatcaaaag aagcagtaaa tcaaaaaaac
    29101 gaggaatgac agacgtcagc ctcgtttttt gattaacgat agttagtgaa ttgcaactcg
    29161 attggtaagt caagtttacg taataaagca atcacagctt gtagatcatc acggttctta
    29221 cctgtcactc taatctgatc ttcttggatt tgtgctttta ctttcagttt ttcatttttg
    29281 atagctgtag tgattttctt cgcgttttca cgatcgattc cgttgaccaa atcggcagtt
    29341 tgtcgagctt tgccacctag agcgtgttct gattcggaaa aatggatatt tttgattggc
    29401 acatttcttt tgatcagctt accaaataat acatctttga tctgttcgat tttgaaatca
    29461 tcatcgctca atacaactag attgctgttc tctaatttga tttctactgt agaatccttg
    29521 aagtcaaagc gattggacaa ttcttttgtt gtttgctgaa tcgcattttt tacttcttct
    29581 gtattgactt ctgatacaat gtcgaagcta gcatcttttg ccataggaac cctcctgata
    29641 tacatataaa tatttaccat acatctatct tatcatagga ttagggcaga ttcttacctg
    29701 tttgctaaaa ttcttctcat ttgagttcga agtattttat tttaatagat aaactgttat
    29761 tcttacattt ggaataccaa agtaggggag aggtgcttgt gagtaaaatt atgactgcgt
    29821 atcataaaaa tgaaagatta aagaaaatag tggttatttt gataaccgga ttgaccgctg
    29881 cggtcgcttt aaattatttt ttgattccag ccaatgtctt ttctgcaggg atgaatggga
    29941 tcgctcaaat catttcctcg cttttatatg aatggtttca tatcaagata gacacaggaa
    30001 catttatttt tcttttgaat atccctgtgt ttgtattagg gtttctcaaa gtaggaaaac
    30061 gagcaacgat ccttagtttt gtgaatgttg tatgtgtatc ggtgatgacg atgctgcttc
    30121 cagtcggaca gatcactgat aatattttga tgaatgccct agtaggcggt gttttattag
    30181 gtcttggcgt aggattatct ttaaaaatgg gctttacaac tggtgggatg gatatcatct
    30241 ctcttgtact ttcacaaaca actggaaaaa cagtcggaaa ctacatgctg attctaaatg
    30301 gtatcattgt agtcatcgca ggcttcttct ttaactggga aagtgccttg tatacgatta
    30361 tttccattta tgcaatgact caagtaattg atgccatcca cacgagtcat caaaaagtaa
    30421 cagcaatgat cgttaccgtc aagccagaag aggtaacaga agaactagca aaacggatgg
    30481 tacgtggaat gacattactg ccgtctgtcg gtggctattc aaaaaaacaa gggaacatga
    30541 tcatgatggt catcacccgc tacgaaatgt atgatcttga tcagatcgtt catgaaatcg
    30601 acgacgatgc atttatcaat attttaccaa ctcattcagt atttggccgg tttgcaaatg
    30661 aaaacgaaca aaaaatgtat cgaacaactg gcgtattgcc agaaataaaa aatcgttcac
    30721 aaaaaaaatg aggaatcagg tttattttag aataaattta atctattaca tctaataaga
    30781 acatttttat aaatttaaac gagaatctta cttctaaatt ctcatttaca agcagctaaa
    30841 aaaagagtat gataaataaa aatcgtgtta aggagtgtcg tacgatgagc gaagcaaacc
    30901 aagaacggtc tggacaagaa atagaactaa tcaaagaacg tattcttgct gccttggaaa
    30961 tggtgattga tcctgaatta ggaatcgaca tcgtcaattt aggcttgatt tatgatatag
    31021 aattcaatcc tgaaaatgga gaaacagtca ttaagatgac tctaacaact atgggctgtc
    31081 cattagcaga tattttaaca gaatctatcc atgacgcatt aaaagaagtg ccagaagtaa
    31141 cgaaagcaga agtcaaacta gtctggtatc cagcatggac gacagataag atgagccgtt
    31201 atgcacggat tgcgttgggg atacgataga aatcttggct gactagctat gagaagcaaa
    31261 taggaaaaac aaaaaagcct tttatccttt ctagaagaaa agagataaaa ggtttctttg
    31321 tttataaaat tttgtctgct atattataag ttaattaaat aaatgtgttc tattgataaa
    31381 ggacattcaa atccctttcc ttttggaatc cttctttttt acatcagttt gattttcgta
    31441 tagtattctt tctcatcttc acgataaatc gcagccccat ttttttgttg tgctttaaga
    31501 ctgccaagat tttttttatt tacagacaaa agtgcctcat caatgccgat tttttttgcc
    31561 tcttgtagta ctaatgctaa tccttgtgtg gcatatcctt ttttacgata attaggggag
    31621 atgccgtaac caatatggcc agcgccgttt cttaatgttt cgttcagttt atggcgcaaa
    31681 ttaaaaattc ctacatactc atgttcatca ctttctaaaa taaagacggt tgcgggaacg
    31741 taactctcag gaagatcgat tcctaaagac gttttttcgc atttttttac ataatctaaa
    31801 aattcagcaa atgtatagcc ataagcattg ttgataaatc cactctcatt tggtccaaag
    31861 ctagtttgta gctgataagc tttctttaaa tctgcttccc atagtttgat cagtttcatc
    31921 gtattcacct ctttaagtaa ccttattcat ttgttttcta ctatagcact attgttgtaa
    31981 atagccatgc ctatttttat ttattgtgtc atatcgtatt aagaatcatc gaacaataca
    32041 aatatgaatt acaataaaaa aattatttga agttcgttca ttcgttactt atgatctcta
    32101 gaagaaagaa aacgtgaatt acttcagcgt ctagaattcc agaaggatag taatcaacag
    32161 aagaaataat caatatccaa atgaaagaag atagctgaat cgagaaaaag aggaaaaaag
    32221 aaatgaagaa aaaaataaaa tagttactaa tcccagagtc gttcgtttaa cattgaaatc
    32281 gatcatatgg ataaaacaga ttttttcacc aaccctcaat agatgatgga gttgctcgga
    32341 tgaattattt gtgtagatgg cataagggag ggcgtacaga aatgaaaaag aacaaaaata
    32401 tgttcttgac aaaaaacaca tgtaatccta aactaaaaga tgtgatgcgg ctatggcgga
    32461 attggcagac gcgctagctt caggcgctag tgagggtgac ctcgtggaag ttcgagtctt
    32521 cttagccgca taatttgttt ttttataaag agaaggtaca cctggagcga tgagaaagaa
    32581 aataacatat tatctaaaag aatataggaa aaattttaaa caacggaacc agattcaaat
    32641 aaatcaaaaa aaatttgtgt gaaggtggga cagtttaaaa cctaatagat tgattcaaat
    32701 agtagattta tagattctga tacaagaaat actggcaaac gatagttaag gaataaagac
    32761 taaacttaaa ctcaaaaaga ggtcatgcga aagaataaat cgcatgacct cttactatat
    32821 tttgaatgtg tttaaacgtt caaataatga atcttagtac caaccgtttg cttcccagaa
    32881 tgctttcgcc gcttcccaag aaccataacg tgaagtaaca tattgttctg ctactctttc
    32941 ttggtttgca gcagagtagt caccatttaa gtaagaagaa tctaattggt aacggccgat
    33001 gtaacgtccg ttcgttgctg tgtaagaacc gcttgattct ttttgtgcga tccattcttt
    33061 tgcagagctt gtacttgctg gagtagcagc agtttctgtt acagcaggag caggttgtgt
    33121 ttcagcaaca ggcgctgctt gtgtttccgt ttgaaccgtt tcttgtacaa caggttgttc
    33181 agcttgaaca ggttcagctt ggactggtgc ttcttgaacg gctgcttgag gttcagcagc
    33241 tgcttgtgca gagccttctg ttgggattgt taattgttgt cctgagtaga tcaggttaat
    33301 atcagaaatt gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt
    33361 ttgagaaatc gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc
    33421 agtcgttccc atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt
    33481 aagtgatgtc atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg
    33541 aggaatactt taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca
    33601 aatccacgtt ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc
    33661 acataatgca gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa
    33721 taacttgaaa gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt
    33781 atttttgctt gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata
    33841 ccagaacatc atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc
    33901 ccttgactta gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt
    33961 tgagctgacc catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac
    34021 agaagtagcc atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca
    34081 cgcaatagtt gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga
    34141 aaatattgat ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt
    34201 tggatcgacc gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta
    34261 atgagataag ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa
    34321 aaatagaaat tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg
    34381 ctttagtaaa gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac
    34441 aagataaagg attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg
    34501 ataaatttat ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat
    34561 tagataaatt aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc
    34621 agcctttaga aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa
    34681 actagaataa tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag
    34741 gcaaatggaa gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata
    34801 tgaagaagcg aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat
    34861 cgcacaagaa tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt
    34921 tggtataaca gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc
    34981 gttttctttt taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa
    35041 ataataagaa aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt
    35101 gtttcccaat gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc
    35161 agtaaaactg aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata
    35221 ataaaaaaag gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata
    35281 aataaagtcc atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat
    35341 ttatccggta aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt
    35401 gaaagaagga gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc
    35461 aataatattc gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg
    35521 aataaaataa atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga
    35581 agttattata gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat
    35641 ctagatacca aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga
    35701 ggaaaaaaat gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa
    35761 agattgatgt aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga
    35821 atcgtaaata tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc
    35881 cgacacgttt attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag
    35941 cattgtcctc ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa
    36001 tttgtttttg acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata
    36061 aggtcctaat tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt
    36121 cttaatcatt tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt
    36181 tgctatcaac atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca
    36241 ataatatcaa ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag
    36301 gagaaaaatc tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat
    36361 ataacaagga ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag
    36421 tggtattttg ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg
    36481 tatacttgat cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa
    36541 accagaggaa gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa
    36601 tgggaaagaa cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt
    36661 gaaaattagc aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt
    36721 tccggagaaa gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga
    36781 ttttattatt cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa
    36841 tccccctgca aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa
    36901 ttgggatggc agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt
    36961 tggctacatc ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt
    37021 atttttcttg atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt
    37081 ctaatccagc aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat
    37141 attctttgac aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa
    37201 agcgagtata ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact
    37261 cttgatctgc gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg
    37321 tagcaggtct gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct
    37381 tgcagtcacg gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt
    37441 tttccattat ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt
    37501 gaattactag cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga
    37561 aaaatcgttg atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt
    37621 ttctttgtca tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc
    37681 attatttgtg gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac
    37741 agaagtccag acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc
    37801 ggtttttgga aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg
    37861 gggaacatag acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt
    37921 aggatcgcca tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa
    37981 ctggttttta gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc
    38041 acagccattt tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac
    38101 ttctgctttc tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat
    38161 tgtatgttct ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc
    38221 tgtgccccct agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt
    38281 tcagctgttt atctttccta ttataaatgt atttttattg gaaatggaag tattatattt
    38341 taatatgtag gaaataatta gtttttaagg aatatgtttt aattttttga aaaaaattct
    38401 tctaaataca acagctgttt taattgtgaa aacgaaaaca gtacgctata atgcctgtaa
    38461 ggttgatttt taactgtaaa taaaaatcaa tgagttaaat ctccaaagaa atagcagtgg
    38521 taatgtggag aaaagttcca gcagtttagg aggattttga tgacggaaga aatgattaat
    38581 ttaggagaac aatatgcatg caaaccaatt ggttttacaa aaacggtcat tggtgaagtt
    38641 gtatcaaaaa tgacaaactg cgccgtggtc aaagttgccc aatgtgctgc agaggaccaa
    38701 gaattattag atgaaaaagc aagcatggtt gttgcaaaat atgatacatt tgaataaaga
    38761 aaacaattgg acgtgcggcg tgctgcaagt ccaatttctt tgagaaggta aatcagtatt
    38821 cttttcctat tttctggaga agaaaaagcg aggcgagttg tcatggtaac attctttacg
    38881 gttctattgt ttttattcgt tgttgtgatg tttttatttc tttggctggt tcgaaaagaa
    38941 attatttatc ggactgtgcg aaatcgatgg gtctacttgg tgataccctt cttagtgtca
    39001 ctagtcattt ggtatacact catcagtcat cctacagcag atgaattggc aaaagggatc
    39061 ttgtcagcaa tgatttttat tagttttttg ttggacagta gaggaatcac agaagaagga
    39121 cttgttttga atagctttga caaaaaaggt gttccttttt ctgagatcaa taaaattgta
    39181 ttatatcagc caaaaggttc taaaatcgta aaaatgaatt ttttccgaaa cggatggcga
    39241 ggaccgatgc tgaaattttc tgcatcatta gaagaattag ttccattttt gtcccaacgc
    39301 ttaaacgatg aggcagagat tgatattatg attgatccag aataaaaaca gaggttgcga
    39361 caagatgttt gtcacaacct ctgtttttta tttcaaataa aattatccaa atacgcggaa
    39421 gcgatcggca tcgttcatgt attctttttc cccagccggt gtttctggtg aaccgaatac
    39481 taattgcgaa cgtagtttcc agtttgaagg gatattccat tcttttgcaa ctgcttcgtc
    39541 aataactggg ttgtaatgtt gcaagtttgc gcccaatcct ttatcaacta atgctaccca
    39601 agtattagct gttgcgattc cgtttgattg ttctgaccaa tctgggaagt tgtctgcata
    39661 taattcaaat tgttcttgta gaccttttac cacgtcagta tctttgaaga ataatactgt
    39721 accataacca tttttgaaac cagctaattt gttttgtgta tttgggaaag cttctgctgg
    39781 agtcaatggg cgaagtgctt cttcagtgat ttcccaaagt ttttcatgtg catctccgaa
    39841 aaggataact gcgcgtgttg attgagcatt gaaagcagtt gggctttctt taattgcttc
    39901 tttgatcaaa gttgttaatt cttcattaga aagtgaaaca ttacgtccta gatgatagat
    39961 tgaacggcgg tttttcaaag tgtcagtaaa agttgtcata ataaaatttc tcctttgatt
    40021 tgcgttttgc atttttttaa ttgattacga aatgaagtat agcaccttac aaaaagtaag
    40081 taaacagata tgctcacaaa aaataagcga agttcaaaaa acaattttaa taagaaaaat
    40141 cgttatgtaa ggattttctt agttatcgct ttcattgttg tgttttagga atttttgctt
    40201 atttgaaaaa aacagctgat atataattat ta
//
LOCUS       DACDUU010000018        40132 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.018, whole genome shotgun sequence.
ACCESSION   DACDUU010000018 DACDUU010000000
VERSION     DACDUU010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.018"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(321..1169)
                     /locus_tag="IWL30_000981"
     CDS             complement(321..1169)
                     /locus_tag="IWL30_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9705765.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1393..3333)
                     /gene="thrS"
                     /locus_tag="IWL30_000982"
     CDS             complement(1393..3333)
                     /gene="thrS"
                     /locus_tag="IWL30_000982"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAP9705766.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3670..3849)
                     /locus_tag="IWL30_000983"
     CDS             complement(3670..3849)
                     /locus_tag="IWL30_000983"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAP9705767.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3896..5719)
                     /gene="cadA"
                     /locus_tag="IWL30_000984"
     CDS             complement(3896..5719)
                     /gene="cadA"
                     /locus_tag="IWL30_000984"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP9705768.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5997..8276
                     /locus_tag="IWL30_000985"
     CDS             5997..8276
                     /locus_tag="IWL30_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9705769.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8369..9016)
                     /locus_tag="IWL30_000986"
     CDS             complement(8369..9016)
                     /locus_tag="IWL30_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705770.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(9195..9914)
                     /locus_tag="IWL30_000987"
     CDS             complement(9195..9914)
                     /locus_tag="IWL30_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9705771.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(10045..11196)
                     /locus_tag="IWL30_000988"
     CDS             complement(10045..11196)
                     /locus_tag="IWL30_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP9705772.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11413..12153)
                     /locus_tag="IWL30_000989"
     CDS             complement(11413..12153)
                     /locus_tag="IWL30_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9705773.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(12157..12480)
                     /gene="rsfS"
                     /locus_tag="IWL30_000990"
     CDS             complement(12157..12480)
                     /gene="rsfS"
                     /locus_tag="IWL30_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAP9705774.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12537..13130)
                     /locus_tag="IWL30_000991"
     CDS             complement(12537..13130)
                     /locus_tag="IWL30_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9705775.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(13114..13761)
                     /locus_tag="IWL30_000992"
     CDS             complement(13114..13761)
                     /locus_tag="IWL30_000992"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAP9705776.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13774..14085)
                     /gene="yhbY"
                     /locus_tag="IWL30_000993"
     CDS             complement(13774..14085)
                     /gene="yhbY"
                     /locus_tag="IWL30_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAP9705777.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(14101..15210)
                     /gene="yqeH"
                     /locus_tag="IWL30_000994"
     CDS             complement(14101..15210)
                     /gene="yqeH"
                     /locus_tag="IWL30_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAP9705778.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(15207..15734)
                     /locus_tag="IWL30_000995"
     CDS             complement(15207..15734)
                     /locus_tag="IWL30_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAP9705779.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15914..16699)
                     /locus_tag="IWL30_000996"
     CDS             complement(15914..16699)
                     /locus_tag="IWL30_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAP9705780.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16692..17561)
                     /locus_tag="IWL30_000997"
     CDS             complement(16692..17561)
                     /locus_tag="IWL30_000997"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAP9705781.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17562..18941)
                     /locus_tag="IWL30_000998"
     CDS             complement(17562..18941)
                     /locus_tag="IWL30_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAP9705782.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18949..19368)
                     /gene="fabZ"
                     /locus_tag="IWL30_000999"
     CDS             complement(18949..19368)
                     /gene="fabZ"
                     /locus_tag="IWL30_000999"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP9705783.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19393..19869)
                     /gene="accB"
                     /locus_tag="IWL30_001000"
     CDS             complement(19393..19869)
                     /gene="accB"
                     /locus_tag="IWL30_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAP9705784.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVISEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19872..21107)
                     /gene="fabF"
                     /locus_tag="IWL30_001001"
     CDS             complement(19872..21107)
                     /gene="fabF"
                     /locus_tag="IWL30_001001"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAP9705785.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(21126..21863)
                     /gene="fabG"
                     /locus_tag="IWL30_001002"
     CDS             complement(21126..21863)
                     /gene="fabG"
                     /locus_tag="IWL30_001002"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAP9705786.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21875..22792)
                     /gene="fabD"
                     /locus_tag="IWL30_001003"
     CDS             complement(21875..22792)
                     /gene="fabD"
                     /locus_tag="IWL30_001003"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP9705787.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEATPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22899..23123)
                     /locus_tag="IWL30_001004"
     CDS             complement(22899..23123)
                     /locus_tag="IWL30_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP9705788.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(23176..24138)
                     /locus_tag="IWL30_001005"
     CDS             complement(23176..24138)
                     /locus_tag="IWL30_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAP9705789.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(24148..24588)
                     /locus_tag="IWL30_001006"
     CDS             complement(24148..24588)
                     /locus_tag="IWL30_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9705790.1"
                     /translation="MESDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24894..25859
                     /locus_tag="IWL30_001007"
     CDS             24894..25859
                     /locus_tag="IWL30_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP9705791.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25916..26146)
                     /locus_tag="IWL30_001008"
     CDS             complement(25916..26146)
                     /locus_tag="IWL30_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAP9705792.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(26232..27119)
                     /locus_tag="IWL30_001009"
     CDS             complement(26232..27119)
                     /locus_tag="IWL30_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP9705793.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(27208..28431)
                     /locus_tag="IWL30_001010"
     CDS             complement(27208..28431)
                     /locus_tag="IWL30_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9705794.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28448..29527)
                     /locus_tag="IWL30_001011"
     CDS             complement(28448..29527)
                     /locus_tag="IWL30_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9705795.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(29658..31385)
                     /gene="ptsP"
                     /locus_tag="IWL30_001012"
     CDS             complement(29658..31385)
                     /gene="ptsP"
                     /locus_tag="IWL30_001012"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP9705796.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(31385..31651)
                     /locus_tag="IWL30_001013"
     CDS             complement(31385..31651)
                     /locus_tag="IWL30_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAP9705797.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            32130..34364
                     /locus_tag="IWL30_001014"
     CDS             32130..34364
                     /locus_tag="IWL30_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP9705798.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34512..34832
                     /locus_tag="IWL30_001015"
     CDS             34512..34832
                     /locus_tag="IWL30_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP9705799.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(35037..35261)
                     /locus_tag="IWL30_001016"
     CDS             complement(35037..35261)
                     /locus_tag="IWL30_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAP9705800.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(35459..37039)
                     /locus_tag="IWL30_001017"
     CDS             complement(35459..37039)
                     /locus_tag="IWL30_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAP9705801.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(37440..38816)
                     /locus_tag="IWL30_001018"
     CDS             complement(37440..38816)
                     /locus_tag="IWL30_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP9705802.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(38944..40062)
                     /locus_tag="IWL30_001019"
     CDS             complement(38944..40062)
                     /locus_tag="IWL30_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9705803.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
BASE COUNT        11410 a         8618 c         6910 g        13194 t
ORIGIN      
        1 tatttttttc ataaaatctt tcatcatttc ctatccttcc gcctatttac attcatgctt
       61 cattgtaccc aaaaacttct taataaaaaa gaaaaaatgg gcggagcaag aaattcttta
      121 cctaatttca agataaaatc aactttttgt ttcacagcaa tcacttaaag ttacatatcc
      181 aactattttt ttcattaaaa aataaactta tttttgaaaa ttatgttttc ttaagtcgtg
      241 aaacactttg atggatcatt tatttgtgga aaactacgtt ttgttattct aaaaaactta
      301 tataaaaaat agtaaggttt ttatttcgaa tccataaaat attcttggat tgcttctcgt
      361 gtttttttca ctttttcgct aagcttttcg tttttagcca tatagaaata aaaaatgata
      421 tcgatcgtag caaattgggc caaaagtgaa ttagttgccg cacttcgatt cgtgatttct
      481 tttggtctag atgtttgtac gagtacatcg gctatcttgc taagcgggtt atttccaaat
      541 tgcgtcaacg caatgatttc gatattcatg cttttcgcca gctcggctaa tgcgactaca
      601 tctgcacttc tacctgagtt tgaaacaagc caaagcactt tctttttctc attgctgaca
      661 agttgcggga gcaagacgtg gatatctttt tcaaaaatga ttggcttacc gacacgagac
      721 catttttgca gaatatcttc tactactaaa gaagaggcgc caaccccata taaaaaaaca
      781 gtctctgccg attccaaaca ctctacgact ttttggatcg tttcttcttc tagaatatct
      841 actgtttctt ttatcgctac ttgtgcattg ttaccaagtt tgtttttgat agagtaaaag
      901 gactcatttg cttctatatc actaaaacct tcatcgattc cagtctgtag ttctgttgaa
      961 atcgtgattt tgaagtccgt cagactttga aaaccgattt ttttggaaaa acgtacaact
     1021 gtcggcgcac tcaacccgga agcttctgct aattcattag ctgtcattgt agggaccttc
     1081 tcaatattac cgataatgta gttggccaac tttttttctg ctgaagaata atcttccatc
     1141 atactttcaa tacgactaat tacgctcata agttcctcca cccttttaat gatagtctat
     1201 gtaagtatac catatcagac ccttattcac ggtcgtatac tggataaaaa acaaaaaaac
     1261 aatccgccca acagaatatc ttatcccatc aatcccacag attctattga gcatctgtgt
     1321 ctttgaggaa tacctgttat ctattgttcg gattgctttc tatctactac ttttttacag
     1381 gaaatttacc tattagtttt ctctgctgta atttttgact tctgcttcca tagcatcaat
     1441 aaagatattc aaatcttcta cagctgtttc tttacttcca taacgacgta cgttcactgt
     1501 tgcatcttcc aattccttgt ctccaacaac taattggtaa gggatctttt gtgtttgaga
     1561 ggcacgtatc ttatagccca ttttttcatt acgatcatca acttctacac gcaacccctt
     1621 catttgcaaa cgttctttga tttcataagc ataatcgcca tgtgcatcaa cagaaacagg
     1681 gatgattgtt gcttgtatag gtgccaacca tgttggaaaa gcgcctttgt atacttctgt
     1741 taaataagcg acaaaacgtt ccattgtaga aacaattccg cggtggataa ctactggacg
     1801 atgcgtattt tccccgtctt ctccaacata tgtcaaatca aagcgctctg gcaataagaa
     1861 gtctaattgt atagttgata atgtttcttc tgtaccaaga gctgttttga cttgaacatc
     1921 tagttttggt ccgtaaaagg ctgcctcgcc ttctgcttca aagtaatcta gttctaattc
     1981 atccatagcg gctttcagca ttgcttgggc tttttcccac attgcgtcat catcaaaata
     2041 tttatctgta ttgtttggat cgcgatagct caagcggaaa cgataatctg taatattgaa
     2101 gtcagcatat actgccacca tcaattcaag cgtacgttta aattcatctt tgatttgatc
     2161 aggacgaaca aatgtatgac catcattcaa cgtcatttct cgtacacgtt gtaaacctga
     2221 caaagcacct gatttttcat agcgatgcat cattcctaat tcagcgatac gaattggtaa
     2281 ttcacgataa gaatggatcg tgtttttgta gaccatcatg tgatgagggc agttcattgg
     2341 gcgaagaaca agcatttcgc catctcccat atccattggc gggaacatgt cttcttgata
     2401 atgatcccag tgtccagatg ttttgtacaa ctcaacatct cccattattg gtgtatatac
     2461 atgttggtaa cctagactaa cttctttgtc tacgatataa cgttcgatcg tgcgtcggat
     2521 tgttgcccct tttggaagcc agaatggcaa accagaacct acttctgggg aaaccatgaa
     2581 caagtcaagt tcttttccta gtttacgatg gtctctttct tttgcttctt cacggagacg
     2641 gatatattct ttcaaagctt tcttgtcaaa aaaggctgtc ccgtatacac gttgcatcat
     2701 ttgattgttt gagtttccac gccaataagc acctgccact gataataatt taaatacttg
     2761 tatacggcca gtggatggaa catgtggtcc acgacacaaa tcgatgaatt cgccttgttg
     2821 ataagcagta atcacttctt cttctggcaa ttcactaatc agttctactt tgtacggatc
     2881 acttgcaaaa atttctaatg cttcctgttt tgaaagaaca cggcgttcga tcggcagatt
     2941 ttctttgacg attttcatca tttctgcttc gattgccggc aaatcttctg cagttacttg
     3001 gttttgtcca ttatctgtat cgtagtaaaa ccctgaatcg atagctggtc caacgccaaa
     3061 gtggatattt ggatagaggc gacgcatggc ttgggccatc aaatgagctg ctgaatgacg
     3121 gactaagcca agtgcatctt catggtctgg tgtgatgatc tcaatacttg catcttcctc
     3181 gattggacga gtcaaatcaa cgagttttcc gttcacttta ccagctaaag ctttttttgc
     3241 taagcttttt gaaatgcttt cagcaattgc caatgttgtt gttccagact caaattcttt
     3301 gactgcacca tctggaaaag taatttttat cataatagtt tcctccttga aatgttttga
     3361 ttgatcagat catcaagctg aaaaacaaaa aaatcctagc atctcttgaa aagagatact
     3421 aggacgaaaa gttcgtggtt ccacctaatg ttcaaacgca aatgcgttcc tcaagcgttg
     3481 taacggtccg accgcccttg cttcaacaag ggtttcgaaa gtggtcatcc tttggttttc
     3541 tcctggaaaa gtttcagcct agcttttcct ctcttatcga agactctcaa attcagttgg
     3601 ctttctcatc aatcaattat gtgtttattt aaccactaat aaatagaaaa atcaagtggg
     3661 aaactaaact tattttttcc attccccatt tacaccgtat gttccttttt tcatctcatt
     3721 gatgatgaca tggatgtgtt cttttggtgc ctgcgtgttt ttatgaacag cttccgtgat
     3781 atctttcatc atattttcta attgttccgg actgcgacct tctactaatt cgacatgtac
     3841 gaatggcata atgctctctc ctctttctga tcgttctttt ttcaactcga ttctatcata
     3901 ctatgtttgt cttgggtact gaatcaggaa aaatggctgg atcttttttt gctctcaagg
     3961 cactcaaaat agagaccgta tccacgactt cttgcaaaac agctccgaat aatgcgggga
     4021 ttagtcccgt acttgcaatt aacatcaaaa aaatacaaat aaagatgcca atcagtacag
     4081 actgtttcgc tattctcatc gtatctcttg aaatcactac tgcttttgct acacgcaaga
     4141 gatcatcttt caagatgacc gcatctgcac tttcactagc agctgtagaa ccatgagccc
     4201 ccattgcaat accgatatct gcagcagcaa gtgctggtgc gtcattcacg ccgtctccta
     4261 ccatcgcaac aggtctttga tctttaggga tactttctaa aatggtcagt ttatcttgag
     4321 gcaagctacg ggcatgtaca gaatctaatt ggactttttc tgcaatcgtt tgagcaatgg
     4381 cagatccatc cccggtgatc atcatcaaat ttttcacatc aagttttctt agctccttga
     4441 ttgtctttgc tgcttctggg cgaatctgat ccgaaaatag gatcgttcct gcaaaagatc
     4501 gatcaattgt tacatagatt gctgtattgg acgtttctat ttcttcagct gaaacaaaag
     4561 aggcacgtcc aatctttact tcatgattat tgactactgc ttggattcct tgaccggtca
     4621 cttctttcag ttgactaaca ggatacagtt ggctgttttt cacgttgttc actaacgaac
     4681 gagctaagat atggctagat tcctgttctg cactagctgc atacagaagt agctcttctt
     4741 tttttattcc attgactggc tgaatcgcct ctacttccaa tatccctttg gtaagcgttc
     4801 ctgttttatc aaaagcaatc gtttttgtac gtgcgagctt ttcaatcgtt gttccagttt
     4861 ttacaacgat cccgttttta cttgaacggc tcattcctgc aactaaagca atgggcgcag
     4921 ctagaatcag tggacaaggt gatgccacta ccagtacctc agcaaatcgt aaagggtctt
     4981 ttgagaacca ccaagaaata ccaccgatca aataagcaat caacgtgaaa gggactgcat
     5041 aacgatcagc taaacggaca aatcgggcag gcttttcttt tgattcttcg actagtttaa
     5101 taatcatttg atactggctg tctgcggcac gtttttcagc gatcatttgg atcgatccgt
     5161 ctccatttaa agaccctgac ataatctgat ctccaggttt cttggtttcc gggcgggact
     5221 cacctgtcaa agaagattca tcgaccgaag attctccttt ggtcactcga ccatctgcgg
     5281 gtacaagttc gccaggcttg actagtaact ggtcatcaat atttatttca tcgattacta
     5341 tatcctgcaa ttttccttct ttgaatcgat gggcaattct tggggtgtta tctaacaaag
     5401 actgcagttc tttacccgct cgtttattcg cgtaatcctc tagactatct ccgcccgtaa
     5461 gcatgatcaa tatcatcaaa ctagcccagt attctttgac ggctaatgtt gcaaggattg
     5521 cggtaatcgc taatacatct acgccatatc ttcctgaacg taacgtcttg atcatttcca
     5581 aaaacatcaa gaaggcgaga atccctccag taaatgcgac aatcgcaaat gcaagggccg
     5641 gctggtgaaa aacaaattca cttaataaag ctatcccacc aataagcaaa gtaagtaaaa
     5701 attttttgaa atgtgtcatt gttttttctc ctcttttatg tgacttagag aggttagtaa
     5761 taaatttcca gctgcctctt tcatcttaag catagtcctt gactagcact ttttaaaaca
     5821 ataggcctta attgaaaaca actatcacta tcagcgtttc caaaattcat cagttctaca
     5881 aacaaaaata ttcagcaagg attacttttt cgattcatca gttaaaaagt accttttttc
     5941 gaaaaactat gctatacttt taattcgtta tttacggcgg aaggagcaaa catgcgatga
     6001 atgagaaaca actagaacaa acgcacgtgg atgaaacgat cgaactaatc cagatcgaac
     6061 aaaatatgct ggatacaaaa caacaagaat tgactcagga aatgaatcat tcgacaaaag
     6121 acacagctaa tcacaagatt cgagctggtt ccaatgaagc tttttatgaa tctgctgttg
     6181 aatatcgcca acatgagcag gaattgcttt tgaaatacca tactcttgaa gcacagcaaa
     6241 aacgcctgca aacgttgtct gtcatgaaag atagtcctta ttttgcccgt atcgacttta
     6301 aagaagaccg tgaaacagaa acattatatc taggtatcgc ttctttaaga gatctggcag
     6361 aagaaacaat cgttattgac tggcgcgcac caattgccaa tctttattat gaaggtgaga
     6421 ttggtcccgc attttatgaa actgaaacag atagaataga tgtcgatctg cagttgaaac
     6481 gacaatttaa aatccaagaa ggacgtattt tatctatggt agatacgtcg gaagtaatca
     6541 atgatgactt tttgctagag atattggatg aatcatcaag caaccatatg aaaaacatcg
     6601 tctctacgat acaaaaagca caaaatgcaa tcattcggga cacaacaagt aaagtaatgc
     6661 tgattgaagg aatcgccgga agtggaaaga catccgcttt gcttcaacgt attgcttttc
     6721 tgttgtatca taatcgcaag tggctcgagg ctgaaaacgt tcttctattt tcacccaacc
     6781 atttgttttc tgattacatc tcgactgtat tgccttcatt aggtgaaagc ggcgtgccta
     6841 cacagacatt caaaaattat ttggtgcagc tgctgccaga gttttcttta ctagatgaac
     6901 agcaacaaga aagtggtttt ttgctagggg aaaaagatcc gatacaagtg atgaaaagcg
     6961 gattgacctt agtcgatcag attgatcgct atatccaagg catcacaaac tacggtccct
     7021 tgttccgtga tatgaaaata aatggccgta cgatcctatc aaaggaatcg attcgccaat
     7081 ggtataaaga aacaaatgaa caattacctt tacatcatag actttctcta ttacaaacga
     7141 agctcctgaa aaaattaggc ggtctgcaaa aagacgaaac acgtcagcaa tgggtcaaag
     7201 atctggcgga agaacagctc caagaactat acgcaacaga tccaaatctt gaatatacag
     7261 aacaaaagga gcgttcatta cgtaaaaaac tggccaacca aatcgtacgt aaaagattcc
     7321 gtcgaatcca tcgagggatc atgaattatc aatttgtcaa tataacgaaa caatatctcc
     7381 actttctaca aactgtgcca ctttctcttc taacacgaca taaaatcagc gagaccaggt
     7441 ggcaggaaca tttgacaact gttcgtacac acttgcgtca aagagaattg cgtcaagagg
     7501 atgctgtact attcttttta ctgatgcgcc gaacatatcc agtgacggtt acacacaaag
     7561 ctcgctatat ctttattgat gaaatgcagg atttcgcacc atcacaagtg gcattgctgc
     7621 aagccttgtt cccaaaagca aatttgactt tctgtggaga tctaaatcaa aatgtctttg
     7681 gaaatgaaac gatcgttgga tcattggaag accttttcgt ggaccgtgaa gtcactcgtt
     7741 tccaattaac gacaagctat cgctccacaa aagaaatcac tgattttgct aatcatttta
     7801 ttgtgaaaga acatcaagta gaaacaacag caagaaaagg agaccttcct ttgctcatcc
     7861 aaggaacaac agatgatatc aaattgaatt ggttagcaga taccattcga acgactcagg
     7921 gaaaagcccg gtattggcgt acagcaatca tagctaaaac acaagaagaa tgcgaagctc
     7981 tttatgctca actgccagac gagttgaaac aacaagtcca gctgatttta aatgagagtg
     8041 actttatgaa gcgttccatt attttacttc cagccttttt agcaaaaggt ctggagtttg
     8101 accgagtttt cttgtgggat gtagatgcag aacatttttc ttctcagcaa gacaaattga
     8161 tcctttacac catgtgtaca cgtgcgatgc atgaattgac cctttttacc tccaataatg
     8221 aaagtccttt gttaaacaat atggatattt catcttatga gaaaaaagta ctgtaagtaa
     8281 aaagacaact gctatcatta atgttagcag ttgtcttttt atttacaata ataattgcag
     8341 aaaaagtaat tcaatcattt aaggtcattt attctatata taaatccaaa tctgtttcag
     8401 gatattttaa tttgacaagc gaaataatca ttttcagttc ttccatgttt tctggatatt
     8461 ctgggtcaaa taccttgcta tgacggccaa tcccgccatt gtcgatcaat cggtaaattc
     8521 gattccattc aatgaattgg tcaattgatt ggaaattgct gattggatag ccgccacaat
     8581 ccaaaatctc tgatacacga aatgctaggt atgcactatc gatcacttta ttttttttcg
     8641 tgtctatctt ctcatttctt tcgataagaa ttttttcagg acgatgtttc aagctccact
     8701 ctaatagtag aaagaaagcc tccatccgat actcttcttc aaataaccga aaggctgaat
     8761 cagaacgacg aaaaagcagc ggaatcagat aattttgtct ataaatcagc attggtgcgc
     8821 ggtccaccat acactctaaa taacggttca gataccctgt accgacatag tttcctaatc
     8881 gatagccttc aacgccgaat cttgcgtatt tttgcgctaa ttttgaacaa tcagttacgt
     8941 tcacagctaa ctcttctact ttgcggtaac cgcttaagat acgttcaaaa ggttctaaag
     9001 tgatttttgc ttccatattc ttccccgtcc atcttccaca tacttcatat catttcacat
     9061 gatacttatg ctctcatcat accacaggtt gaattagaaa agaaaataaa tcacacaaaa
     9121 aagcaagaca atctgttagt gagattgtct tgcttttcga aaaagactga atacttttcg
     9181 ttaaaactgg ttgcttagtc ttctaatgcc acattatgga acacatgctg tacatcttct
     9241 agatcttcca gtgcatcaat cattttttca aatgtttctt gatcttctgg agatagtgtt
     9301 acctcgtttt gagggatcat ttggatctca gcgattgaaa aatcagtgat tcctttttct
     9361 tttaatgctt cttgtatact atggaaatcc gctacttcac cgtaaacgat gatttgtcct
     9421 tcttcttcca ttacatcgcg aacatcgatg tctttttcca ttaggtattc aaggatctcg
     9481 tcagcgtctt ctcctgcaaa agcaaagatt gctgtattgt caaacatgta agaaaccgct
     9541 ccggaaacac ccatgttccc gccatttttc ccaaatgctg cccgtacgtc tgctgctgtt
     9601 cgattcacat tgttcgtcaa tgtatcaaca atcaccattg agccgcttgg tccaaatcct
     9661 tcatagcgca attcagagta tgtttcatct cctgcacctt ttgctttttc gattgctcgg
     9721 tcaataatat gttttggtac attataagtt ttagcgcgat caatgacgaa acgtagtttt
     9781 tggtttgcat gcggatcagg atcacctgat tttgctgccg cgtagatttc aatcccaaat
     9841 tttgcataaa tccggctatt attagcatct ttagcagttt tctttgctac gatatttgcc
     9901 catttacgtc ccatattttc acttcgcttt ctgatttgat agtttcataa cgcaaaaacg
     9961 tcctgttcca ttatacttgt gaatcacgat tctgtgaagc tttttttctt tcttccttca
    10021 ttcttctcat ttttagtcaa ttatctatac tcggattggg atttgcccga aattctgttc
    10081 cggaatattg cctcccaatt gatagatttg atcacttcta actaacagtt ccgcttcttg
    10141 tgcctctttt tttccgattc gggaataaat agaaatctct tttttttctt ttaagaatct
    10201 tcttccagca tcggtaaatc caagtagtcg aagataattt ttattttgtt ggacaggaat
    10261 ctcttgcttt ttgatattca gcaagacata acaagataat cgttggatcc gtgtccatgt
    10321 atatcttttt gtttttacct ttgacacaaa atcctcaaaa ctatcagcag ttgcagctgc
    10381 tgttttcatg cgcgattcta ttccttcggt catctgatag atatctgtca gttccagcaa
    10441 agtagaagat aacaaccgat aacgcaaata cggccaataa gattcccaag tctgcagcga
    10501 acaagttgaa agatcttctt ctgtctgagg cggcacaagt ccagctattc tttttccttc
    10561 ttctaacgca tggcgaatgg ctgttgcact ggctatcctc tgatctgtga tgattttttc
    10621 atgatagcct gcacttttac gtcggatcgg atgtaattcc atagggtgga catacttggc
    10681 attttccttt gcataactta atcccaagat atgatttggc gaagaaaaat caagcgtaat
    10741 atcgggatac agactgcgaa aaacctcagt cattttttgc ggataactat acgtttgatc
    10801 agacagttct tgaaatgcct cttctatcct atcttgattc tcttggacaa agcgtccgaa
    10861 actgctgtaa tcaaacgaag tctcttcttc tgttccaaaa cacaaactgt cacagtccag
    10921 tgtttgcaag atttttactg cacctttagc aaaatagtca gctgactgaa ccgcccattc
    10981 gacgggaagc tccactacta aatccacacc attttgtaaa gcttcattcg cgcgatgcca
    11041 tttatcgatg atagccggtt cgcctctttg caaaaaattc ccgctcatta cagcaatgac
    11101 gacctctgct cctgttattt ttcgtgccat ttccacatga tagcgatgtc cattgtgaaa
    11161 aggattgtac tctacgataa tcccgcagct tttcatgcgc atcctccttc atagttatcg
    11221 ataaaaaaga aatagagaca agtaaaaaaa ggcaaaaatc ggaaaaaata cccgaactat
    11281 ataaactatt ctcgcaaaca caatacttct gcgacgaaaa gctgcactgc aagagcaatg
    11341 cctatatcgt tcggatatat aatttatgtt gaagatttat tcttttcttg ttaactcgag
    11401 caggcaaaac cgctagtctt tgtatgctac aaagaaccag cgctttgatt tctctgatgc
    11461 tgcttcgtct gtaaaatcag aaaatgcttc gactttcgaa aagccgctgt tttccagcat
    11521 tcttaaataa ctttccaatg gataggtacg ctcttgatgt agttcatctt cacgaataaa
    11581 tcgaccagac ccttcttgtt cttcaacgaa aaaggtaaga aaatgttcga tgctgtgttt
    11641 ctcgcttcct ggataactat cccacaaaaa agcaaaatta tccccttgat aatgatagct
    11701 atattctgga aaaacctcat ctacttgata ggtggagtgt acgtcaaata aaaatcggcc
    11761 attttcttct aacgcttgat agacttcatc aaacacttgc tgtacctctt gtctattttt
    11821 catatagcac agcgaatcag aaaaacaggt aattgcctga tattgtccga tttctgataa
    11881 atcgagcata ttgccttcca caaattggat cgatgcgtct tcttcatatg ctcgctggct
    11941 ggcaatcatc aacatctctt ctgaaaggtc taaccctgtc actttatatc catcttttgc
    12001 aaaggcaacc gctaatgctc cagtaccgca tgccagttcc aaaatatttt ttgtgtcttc
    12061 ttgtaaatga cgtttcgaaa aagctaacca ttgctgataa agtgtttcgt ccatcacttc
    12121 atcgtaaatg aaagcaaatg tttcataagc catatgttaa tccaaccatt cactaagatc
    12181 gactagcgga gcatctgacc atagtttttc cagattatag aaatttcgtt ctggcgcatg
    12241 gaaaacatgt acgatcacat ctccaagatc gatcaatacc cattttccgc cttcttttcc
    12301 ttcaatacgt tttacttcat atttgttttc ttcttcttta tcaatgattt cttctgtaat
    12361 tgcgttgatt tgacgttcgc tatttgctga acagatcatg aaataatctg ccaacaagga
    12421 aacatttcga acgtctaaag cgataatgtc ttctgcccgt tttgaatctg ctgcttcaat
    12481 cgcaattttt aattgttctt tactatctat gatgatttcc tcctaatatg agcagcttat
    12541 ttttttgcta cccagtaatt atatgtttcg attgtttttg gataaatctg ttgttcttgc
    12601 gcaagcaaat gggccaatgt atgttttgtt tcaaatgcta cagcttcgtc tagatcagcc
    12661 catgcaatgg ctcgtgcatc ttgtacccca ggaaaatctc gacctggttc aatatagtct
    12721 gctacataaa tgattttgtc aaggagactc atcttagcag ccccagtcgt atgcacccgg
    12781 attgcatgaa gaatctctgc atcggtgatt cctaattccc gctctacaaa cgaagcgcca
    12841 acaagaccat gccagattgc attattataa ttcaataact ccggatcaaa accgtctctt
    12901 acgattacca ttttgaactc ctcatctggt cgttcttttg cataatcatg tgtcaatgca
    12961 gcaatactag ctttttcagg agaagcaccg tattttttgg caagagccac ggctgtttct
    13021 tctactccaa gcacatgctt gaagcgttgt tcgctcattc tcatctgtat tttttgcatc
    13081 aggttttctc tagaataagc agtatattca ttactatagt ccatattcat acaacccttc
    13141 attttgaata taatcaatta ctttatctgg aacaaggtag cggatagaac agccttcttt
    13201 gatcttttga cgtattttcg tggaactgat gtcgatctca ggtacatcta cccaaattac
    13261 aggatatgga gtatccgttg tgtatcctgg tcgacgtata cccacaaagt tgaccataga
    13321 agtcagctca tcgattttat accattttgg caaatattct accatatccc caccgataat
    13381 aaagtaataa tctgtatccg gattgttttg cgtcaattcc ttcatagtat cataggtgta
    13441 acttttccca ccacgagcta attcaatcgt ttcaatctgc aaaaaaggat tgtcttccac
    13501 cgctaattcc aacatgttga gtcgatgttt tgcatcaatc gtctgtttct catcaacatg
    13561 aggtggctga taagaaggca tcaaaaatac gcggtcaaga cctaaattcc ggccagcttg
    13621 ttcagccatc actaaatgtg caaggtgaac gggattgaat gttccgccca agatccctac
    13681 ttgctttttc ttctctaaga attgaggcat ttcttcagga aataattgtg aacgaacaaa
    13741 tgtcttagct tgcgtattca tcatggtcac tccttatatt gcttttactt cttttgatag
    13801 cctttgatac ttttcttttg tggaaggttt gtataataca ataacacgtc caatgatttg
    13861 gacgatttcg caatctattt ctttttctaa tacttgagct gcttcttctg caatctcatc
    13921 tgtattttga agtaacgata ctttgatcag ttctcttttt tctaaagctt cttcaatttg
    13981 aacgatcatt gctgaattga tcccaccttt tccgatttga aaaatcggct gaagatgatg
    14041 cgctttgcta cgtaaaaaac gcttttgttt tcctcttaaa ctcataattt tccttcttcc
    14101 ttaaatcaat gctttgcgtt tcaatacatc tacacccttt ggcgcccagc ccgcaattac
    14161 tactggcgct gttacagtga tccagcctag accggcaaaa acgatgtctg ttttttcttt
    14221 cacagaaaac tcaaaacgaa caagctccgg aaatgcctcg acttcatcag gacgaggagg
    14281 ctgtaataat ccacctacat gtttttgata aaattcgtct gctttttcca gtttcgttcg
    14341 atgaatattt acatcatttg acacataagc aataaatgaa gcacgattgc cgctgataaa
    14401 atcaaatctt gcaagtccgc ccaagaaaag tgtttgccct tcattcagct gataaacttt
    14461 aggtttgatt tcttttaacg gtgcaatgat ccgcaaatct tttttaccta agtagtgcgc
    14521 catttgatgg cgatggataa ttcccggtgt atcaatcaaa aaatgcccgt catctaaagg
    14581 aatctcgatt ttatccaagg tcgttcctgg aaactgcgaa gttgtgatta catcttgtac
    14641 tcccgctgta tttttgatga tttgattgat aagcgtcgat tttccgacat ttgttactcc
    14701 gacgatatag acatcccgtc catcgcggta ttcttcgatt ttttctaata gttccgtcat
    14761 ctcatgagat ttcttagcac tagttaataa tacatctact ggacgaattc ctgcttcgta
    14821 agcacgttcc ttcatccatt gtgttaattt agtttttttc aatgatttag gtaatatatc
    14881 cactttgttc ccaactaaaa gtactggatt gtcaccgaca aaacggtgta gtcctgggat
    14941 tagtgaacca ttgaagtcaa aaatatccac cacattgacg atcaatgcat ctttttgccc
    15001 taattcattc agtaagcgca aaaagtcgtc atccgtcaat gagacatctt ggatctcatt
    15061 ataatggcgt aatctgaaac aacgctgaca atagacttcc ccggaagcta aacctttttc
    15121 caaagccgtt tgcggcgtgt atcctaattc accgggtttg tttgtttgta taacggcacc
    15181 acagccgata cattgtaatt cttctgtcat tcgattccac ctcgccagcc catatcaggg
    15241 tgttttgcta atagataggc cataattctt ttttcccaaa aacgattaaa ttgtgtcttc
    15301 caactatctg ttgcgataat gggttttaca aggatgctac gtatccctgc acgattggct
    15361 ccacgtatat cagtcataat ctgatcgcct accattacta tctcatcatc tgctaaatcc
    15421 aattgtttct ttgctatgtt gatccctctt gctaaaggtt tcattgctcg tgcaacatac
    15481 aaaagatcaa atttttcaat cgctcgcgcg acacgttttg aattattgtt tgaaacaaca
    15541 acaacaggga ttcctgcatt tttcatttct aaaatccaag taagcaactc ttctgttcca
    15601 tctggattat tccatgcaat caacgtattg tctaaatcgg tcaatactgc tttgatgcct
    15661 agattcttta attgggcagg tgtaatctga tagatggctt ctaccatcca tgttggtttg
    15721 taatttgaaa acatgctgtc tcctcatctg caaaaaatga aggagcacat aaagtactcc
    15781 tcaaccccgt attatactgc atatcagcaa aaaaaactac ctctatactc gttcgtcttt
    15841 cagaaaaagc atttctttac aaaatgacca aaaataaaga ttgatcacag aaaaatttag
    15901 aaacaaaaaa caactaatat tggcggaacc tcttgtatcg tttttccagt aatttatgct
    15961 gatctaatcg aacaagttcc gtcaatcttt caatcaacgc ttttttcata atgtgtgcga
    16021 ctttatatcg tggcaactgc tcgccgttga acgtttcagg aataactcga tcaacgattc
    16081 ctaactcgcg tagttctggc gcagtaattt tcatcaactc agctgcttct gcggcgcgac
    16141 taccgtcttt ccacaaaatc gacgcaaatc cttctggaga aagcacagca taaatcgttt
    16201 cttccagcat ccagacttca tccgctaaac ctagagcaag ggcaccgcca cttccacctt
    16261 caccaatgat gatggcgatg atcggtacct ttaaatcaga catttcgagc agatttctgg
    16321 caatagcctc tccttcgccg cgctcttctg ctcctacacc gcaatatgca cctgctgtat
    16381 tgatcaaggt aatcaccggt ctattgaact tttccgcttg tttcatcagt cgtaaagctt
    16441 ttcgatagcc ttcaggatgg ggagaaccaa aatttctgcg gatattttcc tgcagatccc
    16501 gtcctttttg aataccgatc actgttaccg gtttgccatc taatcgagcg attccaccaa
    16561 caatcgcttc gtcatctgca aataaacgat caccatggca ttcctcgaaa gaatcgaaga
    16621 tctcttcaaa aaaatccaag cttgtccatc gttctttagc tcttgctaac tgaacgacat
    16681 catgtgctgt cttatccacg atctatccat cctttctgtg tatgcaattc aagaagcgta
    16741 tggattttct gtttcaattc catgcgggga acaatctgat cgacaaaacc atgagataat
    16801 aagaattcag ccttttggaa atcttctgga agttcctgct tgatcgtctg ttcgatgacg
    16861 cgtcttccag caaaaccgat caagctttgg ggttctgcta gaataatatc gccttccatt
    16921 gcaaaactgg cagttactcc tccggtcgtc gggtccgtta aaacagttag ataaaaaaga
    16981 ccttcattgc tatgccgttt gacagcagca gagatttttg ccatctgcat tagagaaaag
    17041 attccttctt gcatccgtgc gccacctgaa gctgtgaaca agacgactgg aagactttcc
    17101 tttgttgctc gttcgaataa tctggtgatc ttttctccga caatcgttcc catacttccc
    17161 atgataaaat taggatccat cactccaata atgaagggaa tcccctgaat ggttgcttta
    17221 ccagtgagga ctgcttcatg aagtcctgtt ttatcttgca tttttttaat tttttccgta
    17281 tattcgggaa atgctagcgg atctttggtt acaagatccg tatcccattc ttcaaaactt
    17341 ttttcatcaa taatcaaagc tagacgctgc catgcaccga ttcgaaaagc gtatccgcaa
    17401 tgagggcata ctttttcttc accgatgtct ttcgtataaa ggatatgttt gcaatttgga
    17461 cactttgccc acatgttatc aggaacagat ggcgagttag caggtttgct ctctctatta
    17521 ggattgatcc gaatatattt ctttttctta aacagagcca tctatgcgcc tcctatctcc
    17581 gtttctggtg tccaattcgg taaaaattct tcttgtaaaa atgctgtgct gtaatcacct
    17641 gctatgactg caggatgact aatcaaatcc atctgaaatt ctgcgttcgt gatgattcct
    17701 tctgtgataa attcacttaa ggctcgctgc atcttcatta atgcttcaaa acgcgtattt
    17761 ccatggacga ttatttttgc aatcattgaa tcataatatg gcgggatcgt atatcccggg
    17821 taaactgcac tgtctactcg cagtcccatc ccacctgctg gaagcagtaa gttttggatc
    17881 ttccctggag aaggagcaaa atggaaagca ggattttctg cattaatccg gcattcgatt
    17941 gcgtgtcctt ggaatactac ttcttcttga cacacattca aaggctcacc ggctgctatc
    18001 tccacctgtt tttttactaa atcgattcct gtgaccattt ctgttactgg atgttccact
    18061 tggattcgag tattcatttc catgaaataa aaatcgcctt cttcatccat cagaaactcg
    18121 atcgttccgg cattttcata attgactgct ttagctgctc gaacagcagc gtctcctagt
    18181 gcttctcttt ttgtttgtga gatcactaca gaaggagatt cttccagtac tttttgattg
    18241 ttgcgttgta aagaacaatc tcgctcgcct aaatgaatga catgaccaaa tcgatcacct
    18301 aagatctgaa cttctatatg gcgagcagga taaatgattt tttccaaata catatcatca
    18361 tttccaaatg ccgctgctgc ttcttgctga gctgaagaaa aatgctgtgg gagctcttct
    18421 tttgattgaa ctttccgaat gcctttcccg ccaccacctg cagctgcttt cagcatcact
    18481 ggataaccaa ttctgtctgc tatttccaat gcttcttcta ctgtagataa tacaccagta
    18541 cttcctggaa tgactgggac atttgcttct tgcatcaatt gacgcgcatt gatcttattt
    18601 cccattgcat cgatcgttgc acttttagga ccaataaatg tgatattgca ctcttcacac
    18661 atggaggcaa atcggctatt ttcagataaa aaaccaaatc ctggatgaat tgcttcggct
    18721 tttgtcacga ttgcagcgct cagtacttcc tgaacattca gatatgaatc aacggctttt
    18781 gccgggccaa tgcagattgc ttcatctgct aactgtgtat gaagtgcttc ggcatctgct
    18841 tctgaatata tcgctacggt ttggatccct agttcgcggc atgcacgaat gatacgtaca
    18901 gcgatttcac cgcggttagc tatcaatact ttagaaaaca tatttcacct atccaatcat
    18961 aaaggttaat tccgcttcgg ctactttttt accgtctact ttagcgattc ctttgcctat
    19021 tccggctgaa gctttcactt tcaatatctc aacttcaagg tataaggtat ctcccggtgt
    19081 aactttctta cggaattttg ctttgtccaa tccgccgaaa taagccgtct ttcccttgaa
    19141 ttgttcaaga gaaagtaatg caactgctcc tgcttgagcc attgcttcaa caatcaatac
    19201 ccctggcata acaggttctt ctggaaaatg tccttgaaaa aagggctcat tgatcgttac
    19261 atttttctta gcaacgatcc gttctccttc aaccatttct tccactcgat caatcaaaag
    19321 cattggataa cgatgtggaa tgatttcttt tatttcttgt atgttcattg ttttccctct
    19381 ttcctgcttt gcttattttt gctgaatcct aaagagtggc tgtccatatt cgacaactga
    19441 ctcgttttct acaaggattt ctgtgatcac tccgtcggcc tcactagtta tctcattcat
    19501 cagtttcatc gcttcaataa tgcagataac ttctcctttg gcaacttgat cacctacttt
    19561 tttgaaagct ggtttgtcgg gcgctggttg cagataaacg attcccacga ttggcgaaac
    19621 aacctcgatt ccatcaaata ctgccgattc tttttctttc attatttctg gagcagttgt
    19681 tgcttgttca gatatgacat tgactggctc cgaaactggc aacgtttcgt ttgtttgaac
    19741 catattcatg cctcgacttg ttttattttt attcatatac aattcaaatt gaccttcacg
    19801 caaatcgaat tccgttagcg aggattgatc aaattgactg actaattctc tgatttcatt
    19861 gatatccaaa attattcctc ccagcgtttc atgcacagaa ccgcattatg tccgccaaac
    19921 cctaatgaat tactcattgc ataagtaaaa cttgcaggtt tgctttcgtt tttgacaact
    19981 gtcaagtcaa tctcaggatc ttgtttttct acattgatcg taggtgggat aaattgatgt
    20041 tctaatgcca gcaaagttgc caaggcttcg atcccgccgg ctgctcccaa taaatgtcca
    20101 gtcatcgatt tcgtgctgct gacgtaagta tctctgaaat tttcacctaa tgcgatttgg
    20161 attgcttttg cttctgcctg gtcattggct ggtgtacttg ttccgtgagc attgatatat
    20221 ccgacttgtt ccggctgtac accagcttct tccatcgcaa gcttcattgc tttagatgca
    20281 ccagaaccat ctggagtagg agctgtcata tggtaagcgt cacagtttgc accgtatcca
    20341 acgatttctc catagatttt ggcaccgcga gcttgtgcat gttctaaaga ttcaagaact
    20401 agaacgcctg ctccttcacc catcacaaaa ccattccgat tttcgtcaaa cggaatcgaa
    20461 ccacgattag gatcagttga ttctgagagt gctgttaaag ctgcaaatcc agcaatcccc
    20521 atctcacaaa tcgttgcttc tgttcctcca gcaagaatga catcagaata gccatgtttg
    20581 atgttacgga atgcttcccc aattgaattc gttccgctag cacaagctgt tacgatagct
    20641 gtacaaatac cttttgcacc tacacgcaaa gcaatatttc cagctgccat gttggcaatc
    20701 gccattggta cgaacattgg agaaactcgt tttgcacctt tttcatgtag acgaataact
    20761 tggttttcga ttgttggaag accaccgatc ccagatccga tgatgacgcc aaaccggtct
    20821 tgattcattt tttctttatc taaaccgctc atctccattg cttctaaagc agcgtagatt
    20881 ccatagatcg aaaacatatc cattcgtttg ctatcttttt taataaaata tttatcgtaa
    20941 gggaaatctt tgacttcacc cgctaaggta atccctgttt ctgaagcatc aaatttagtg
    21001 atcggtgcga tgccattcgt cccctctttt aagctattca aaaagctgtc tgcgtcattt
    21061 ccaataggtg aagtgactcc atagccagta attaccactc gattcatttg tttgctctcc
    21121 ttttcttatc cgtgcatgac caatccgcca tcgacattga tgacttgtcc ggtgatatat
    21181 ggattttgtg ctaagaaaac tgctgtttgt gcaatatctt ctacttgtcc gaaacgctgc
    21241 atcggaattt gcttttccgc ttgttccttg actttgtctg ctaaaacttc cgtcatatca
    21301 gttgtgatga atcctggcgc aatggcatta catgtaatgc ctctagttgc agcttctcgt
    21361 gccacagatt ttgttaatcc tactacgcca gctttactag ctgcataatt tgcttgacca
    21421 atatttccaa tcaacccaga tacactagag agattaatga tggccccttc tcgctgcttc
    21481 atcatttttt tcaatacgtg ctgcgtcata ttgaaggttc caatcaaatt gatatccaga
    21541 cattttttga aatcttctgc atccattcgc atcacaagtt tgtcatttgt gattccggca
    21601 ttgtttacta acacatggat cgatccaagc ttttcttctg cttctttgat catttgtccc
    21661 gctttctcat aatcagagat gtcaccggaa actcctacgc attttacacc gaatgcttcg
    21721 atttcttcta tcttttcttt tggaatctct ccgcgaccat tcaaaatgat atttgctcca
    21781 gcttttgcaa aagctaaggc catcgctttg ccgattcctc ttgtactacc tgtgataaat
    21841 acattttttc cttttacatc cataacgttc ctccttatag tattgctatt gtttcatcaa
    21901 atgtctgctt attctcaaca cgcagaactg gtattgtttt atcgattttt ttcataaatc
    21961 cgcttaatac ttttccgggg ccgacttcga tgacttgttc gatgccgatt gtttttaact
    22021 tatggatact ctcatagaaa cgaacaggtt gcatgacttg ctgttcaagt agtcccgcga
    22081 ttgtttcttt cttcattatt tccgttgttg tgttgcttat gataggaaaa gaaggttcag
    22141 agaattgaat ctgacttagg tccttcgcca gtttttttgc tgccggttct aaaatagctg
    22201 tatgaaaagg cccgctgaca tttagaggga tcattctttt gaaccctttt tcttttaata
    22261 gtaaaacagc ttcgtctact gctttttctt cgccaccaat cacgatctgc tgaggtgtat
    22321 tataatttgc aggagatacg atgccgtact tgctagcttc atgacagctt tcctcgatcg
    22381 tttcaatcgg cgcattcatc actgcaacca ttttcccgct tccagctggg gtagcttctg
    22441 tcatataagc gcctcttttt gctactaacg caactgcttc agtgaaggat agcgcattac
    22501 tagctactaa tgccgaatat tctcctaaac tcagtcctaa agctgcatct gggatgattc
    22561 catgctcctt caataagcga taatatgcga tgctgactgt tagaatagct ggctgtgtat
    22621 attgcgtctg atccaaacgt tcattttctg taaagcaaag ttcagccata tcgtagccga
    22681 gaatctcact tgcttcatca aatgtctgct ttacaacggc ttcttcatat aaatcttttc
    22741 ccataccttg atattgtgca ccttggccac taaataagaa tgctgttttc atgtttttct
    22801 ccaatatatc cagaatatat gtttgattct tcctattcaa aaaggccgag acattcctat
    22861 ttcaggaaat gtcccagccc ccaacctaat tttaatgatt atgcagtttg tttttctacg
    22921 taagtcacta aatcttgaac cgttttgatt ccgtcttctg tttcgatttt cacgtcaaat
    22981 tcatcttcaa tttcattgat gatttggaat aaatccaagc tgtcagcttc taagtcttct
    23041 tggatgtcag tagtcaattt gatttcttca gtttcttttc ctaattcttc tgcgatgatt
    23101 tcttgaattt tttcgaatac cataataata tattcctccg atattgtttg ctttttattt
    23161 ttataattta acagtctaca actccatgag aatacttccc catgttaaac ctccgccata
    23221 gcctgtaaat attactttct gctgttttcc taaggtgatc tttccactag aaactgcttc
    23281 atccaataag atagggatgc ttgcagcaga tgtattccca tatttatcca tattggtcag
    23341 gaacttttct tgcggcatct ttacttttcg ggcaattttc tcaataattc tgaggtttgc
    23401 ctgatgcaaa agcagataat caactggttt gtctttcatg acttcttgga tattgtttgc
    23461 aacgtctcgt acggcaaaat caaaaatatc tctgccagtc atttgcaaat aatacgaacc
    23521 ttctgaatct tctgaataga acggattttt attttgctgg tagccagcgg tcaatgagct
    23581 acttctggta ccgtcagctg ctaatttttc tttcagcaat tttttctgtg gattcgcttc
    23641 taagagaatc ccacctgctc catcgccaaa caacacagca gtcgtacgat cagaccaatc
    23701 gattagctta gatagtgttt caccaccaat caccagtccc ttagttgagc cgcagcggat
    23761 cattttttca gccatgctta acgcataaac aaatcctgag cacgcagcac tgatatcaaa
    23821 agcgatagca tgagttgctc ccaatttccc ttgtacttgt gctgctacag atggtgtcgt
    23881 gtaatcgggc gacattgttg ctactaaaat aaaatccaaa tctttagggt ttagattttt
    23941 ctgttttatc aatttttccg ccactaaaat gcataaatca gaagtctcct gatttgttac
    24001 acatcttcgt tcacgaatac ccgtccttga cgaaatccat tcatcagagg tatccatcat
    24061 cttggaaaga tcattattag taataatctt ttcaggaagg acgctggctg ttgcagtaat
    24121 cattccaaat tgttccatta aatatcctca cttatagtct tgcaagaagt cgtgcaagtt
    24181 tttcagtgca tccagcaagg catgctgttc ctccgtagcc attccgtcta agatgttctt
    24241 gaccatttca cgatgaaaat gctgatggac ccgaaatagt aactttcctt ttttcgttag
    24301 tcccaacttt acaacgcggc ggtcatcttc actgcggata cgttcgacat agcctttttt
    24361 tactaacttg ttaatagcag tggtcaaggt cccaacagta attgatagtt ctttggctac
    24421 ttctgaagtt gtttttttct tgtacatccc gattgcctcg atcgtgtgca tctctgtgat
    24481 tgacagatct ttgaattgag attttttcag ctcagactct tcgattgtca aaatatcatt
    24541 gaagacacta acaagataat catttactgt ttttaaatct gattccatgt tccaccgtcc
    24601 actaattagt ttgattatca aatcttttga tgttcaaact atatcttaac ttttttcaaa
    24661 atgcaacctt aaaagatcat ttttttacta tatgagcttt ttttaagatt ttactcattt
    24721 aaaacaactg ataaatcagc atttataatt tcttttatta gaaaaatcat ctatctcaca
    24781 tattttgatt atcaaagtat tttctgtgaa cagaactata gaattcccta aaacttgtcc
    24841 attatttcaa aatttgttat agtcttttta ttcaaagcgt aaaaggagat cctatgttaa
    24901 aagaaaaaat gaaaactctc cccatgctgc cgggtgtcta tcttatgaaa gaccgagatg
    24961 gtaaggtcat ctatgttggt aaagcaaaac ggctcaaaaa tagagtaagc agctattttc
    25021 atcagaataa acagcattca aaaaaagtat tacgtatgat tcatcatata acagattttg
    25081 atttcgtcgt agtagacacg gaattagatg ctttattgct cgagtgtcaa ttgatccaac
    25141 attaccgtcc tttttataac cgacaaatga attatttcag taattataat tatgtacata
    25201 ttaccaataa gggatttgta ttaacagata ctcctacggc tcgaacatac gggccttttc
    25261 gtctctataa aaaaatgccg tctattttgc gtataatgga agaaacttat caaatgcctt
    25321 ggttgtctga aattagtttg ttagctttgc gagtccagct tcctgaccta caggaaatga
    25381 cttttgaaca gaaaaaaaag gaactccaag ggctgtttca aggccgaaat aaaaagctac
    25441 tgacttactt gaaaaaaaga caacaacatt tcatctatca gttgaatttc gaaaaagccg
    25501 gtatgcttca aaaagatatc gaattagtta cttattttat tagacggatc caggaacaaa
    25561 agcagttttt aagaacacca tcgctcacct tcagcatgcc attagcagca gatgagagtc
    25621 agaaaaagca ctacctcgtt tgttatggtc aacttgcaga aacaatgatt acctctggtg
    25681 acgtttctcc tgacttctat tatgaaaaga aagaagctca tttatctttg aaacgtcaat
    25741 tgtcaaaaga agaaatcgat cctgtacaaa tcttgatcag ttaccgcaaa aaattagaaa
    25801 aagaacagtt tgaaattgaa ctgttaaaca aaaaagaagc tgaaaagcaa ctaaattagt
    25861 tgcttctcgg cttcttttag gcatcttcaa cttcaaatgt caattatcct aaaatttatt
    25921 ggattaagtc atagatttcc atcgctacga tatcgatatt atcaaattcg tattgctgat
    25981 gagtatctgt ttcagttaat tcaaatgtat cggtcgcttt atcataagtt actgtacact
    26041 tctctacgcc ttctttttca aaacggcgaa cttgcagttc attatctgct gactcgacca
    26101 tagcctccag tcgttttatg attgcgacta attgtgaatg tgtcatcgga ggtcctcctt
    26161 ttcttatttc tgttatattg tatcaaaagc atagcctact tgcattgatt ttctctaaaa
    26221 aaactcgttt tttatgattt tttttctggt atttttcctt caggccacca aagtttgatc
    26281 aaagcttggg ttccgtcatt ctcaaaacct ttatctgcca actcttcata tagtttcaat
    26341 gcttgggtag ttgctggcaa tacaagatcc attttttctg cttcttctaa tgcaatcttt
    26401 aaatctttga taaaatgttt gacaaaaaaa ccaggtgtgt aatcttcttt taaaatacga
    26461 ggagaataat tgcttaaaga ccagttagct gcacttccac ctgaaagcgt ctcaatcact
    26521 ttctttaaat caagtccgtt tttttgtgca tagacaagca tttccgtcat tcctgtcatc
    26581 gtacctgcaa tcatgatctg attagccatt tttgtatgct gacctttccc agcagaacca
    26641 tgcaatgtaa atgtcgtacc aaattctttg aataaaggca gtacttgatc ataaacggct
    26701 tcttctccac cgaccatgat cgtcagtgta ccattctttg ctcctaagtc accaccggat
    26761 accggtgcat ctaacgcatc tgcgcctttt tcttttgctg tttttgcaat tttttctgcc
    26821 aggcttggtg tacttgtagt caaatcaact aaaatatgtc ctgagatatc tgcagaaaaa
    26881 atccccgact gtccatagta gatttcttct acatctttgg ggtaacctac cattgtaaag
    26941 agaatattac tttgttctgc taccgctttt ggtgtatctg cccagatagc tccttctgtt
    27001 actaaaggat cagcttttgc ttttgtccga ttatagacag ctacttcgtg tcctgcttct
    27061 agtagatggc gtgcgacagc acttcccatg acgcctgttc cgataaaacc aagtttcatt
    27121 ttttcgacct cctaaaaatc aaaaatcctt gcttctatgc tagtatacgc tagaaacaag
    27181 gattttttca taaaaaatgt ttgaatttta tcttttaaat gaatttaatt ttattttgat
    27241 tcgttcaatc gagtcttctt cttcttttct tcgcatctct tcttcatgat aaacaaccat
    27301 gtcttgataa aattcgatca tagcttcgcc gaaatgtgta gatgaaatat cataaagttt
    27361 agcttctaaa acttttggat ctttaggaac ttcactggaa atataatcaa taagtgctgg
    27421 tgcaaagtct tcgtcggttt caaaagtttt ccctaatgaa ggatgatcga acaatcggtt
    27481 caaataagca tttccttcca cgacacaagg tgttccggct gccatcgctt ctgtataagt
    27541 aagcccttgt gtttctgaag tagaagcact gacaaaataa tctgccgctt tataataaag
    27601 tgctacatca ttgttgtcca cttctcctgt gaactgcaca aattcttcta actgcagttc
    27661 tgagatcaat tcctctaact cttctacata aggtcctttt cctacgataa ctaatctggc
    27721 gttaggataa gcggagatga cctgaggaaa cccattaatc aaggcttgga tatttttctc
    27781 ataagaaatc ctgcttaaag aaagaatcat caaatggttt tcctgaagac ctagtgactc
    27841 ccttaatttg ctgatatcct gctgagtgat atcagaacgt ttgaattttt caatgtcgat
    27901 accagtagga atcacgcgaa gcggtgctat tacaccatag ctgcgtaatg tatcaataac
    27961 acgttcactt ggacaaacta cacctgtcgt atgatttgca aataatctag acaagtattt
    28021 tacatgggat tgcctgatca cttttccttt accgatataa tgaaggtagt cttcatacat
    28081 cgtatggtaa gtatggatca gaggtatttt caacttctta gctaccatct tcccaagtat
    28141 ccctgctccg aattctgtat gggtatgaat aagatctagt tctagttctt tggcaaccaa
    28201 ataagcatac cacatgcctc taacaacaac tcttcgatcc ttgaaagaaa taaatggtac
    28261 acttggcatt cgaatcacat ctttttccaa cttatccgca ttaggatcgg tcgttgtaaa
    28321 aatatagact tcatgcccat gtctttctaa ttcttgtttt aatgttttga tcgatgtagc
    28381 aactccgctg acttgcggaa agtaggtatc ggtaaaaaag ccgattttca tgtttttcgc
    28441 ctccaaacta gtccttgacc tgatttactt tttttaattt gctagagact ttttcattca
    28501 gtacactttc gtatacattt ttaagttctt caccgatttt attgatactt cgctcttttg
    28561 ctgtttgata accggcttgt gtcgtactcg gtagttcttg attggtaatc tgttggatca
    28621 gtcggataaa ttcttcgttt gtcttcccca tatagcagtt cttgctatct tctaaccagc
    28681 cattatacac tggaatatct cgaacaagta cgttttgttt gcttgctaaa gcttctaaaa
    28741 caacgatccc ttctgtctct tcataagaag ggaaaaagaa tagatctgca cctgaatacg
    28801 caccttctat gatctctcca cgaatgtaac caggaaacag gacattttca ggatgatctt
    28861 cttttacaat tttccggatg ttccgaggaa tcgagtacat gggaacatga ccaaaccaaa
    28921 taaatgtgta ttcgggcatc ttcttagcaa tttctacgaa atcaatgatt cctttacgtt
    28981 cgaaaaacaa accgacacaa atgattactt tttgatccgg agacagtttg aaatattctc
    29041 tgaacttttg ttctttcatt ggatcagggt gatatttctc taagtcgatc ccattggaaa
    29101 tagcttgaat cggcacacgg ataccatagc tttccaacaa ctgttttgaa taaggtgttg
    29161 gcgtgatcag atagtccgct ttttggtaca atcctaccag atattttttt acgataggag
    29221 aaagctggtt cgatccgata aaggaatttc ggaaatcttc ctctgtagaa tgcgcatgat
    29281 agacaacttt ttttccttct cttctggctt tattgaccat attatgacta ttgattccgt
    29341 atgtattgat gtgcaggatg tcataatctt ctttttcatc tagtgtataa gagataccga
    29401 cttccgtcag agcacgtttt tgatgatcaa gcgcacgccc gattccagat tttgcaagca
    29461 tcttttcact ttcaaaatat aataatacct tcaccttacg tcccccttca cttatatagt
    29521 ataacatagc ctacaaactg tcactcaaac ttaagcccta tttttgtcta ttcttactga
    29581 caaaaacgtt ttcttaaaaa agaacaaaaa aactggcgta gctcttttac aagccacacc
    29641 agcttttgat acgttgttta ttttacatat tcatgaacaa gttcaacgac ttcttccatc
    29701 atatcgcatt ctttcaatgc acgatcagca agttcagcca ttttcgctgt gtccaagcgt
    29761 ttcattaaac tacgcgtttt aagaatagat gtcgcactca ttgagaactc atctagaccc
    29821 atacctacta gcaatggaac agctgtttga tctccagcca tttctccaca catacctgcc
    29881 cattttcctt cagcgtgtgc tgcatcaatt acatttttga tcaaacgtaa aattgatggg
    29941 ttatatggtt ggtacaagta agaaacgcgt tcgttcatac ggtctgctgc cattgtgtat
    30001 tgaatcaaat cgttcgtacc tacagagaag aaatctactt cttttgcaaa cttgtctgct
    30061 aagactgctg ctgctggaat ctcgatcatg atgccgactt ggatcgtatc agatacttcc
    30121 ttgccttcac ttatcaattt ttgtttttct tcttcaaaga tcgcttttgc tgcgcggaat
    30181 tctttgagtg tagctaccat agggaacatg atacgtaaat taccatgaac agatgcacga
    30241 agcaatgcac gcatttgtgt acggaacatt tcgtcgcctt gttcagataa actgatacgc
    30301 aatgcacgat atcctaagaa tggattcatt tcatgaggta attgcagata tggtagttct
    30361 ttatctccac cgatgtccat tgtacgaaca actacaggtt ttccttccat accttctaat
    30421 acagctttgt aagcttcata ttggtcatct tctgttggga agtctggaga atccatatac
    30481 aggaattcag tacggtacag accaactgct tctccaccat tattatggac accaactaga
    30541 tcttttggtg ttccgatgtt tgcagctaat tcaaaatgct tgccgtcagc tgtgacagtt
    30601 tctgcatttt ttaatttttc ccattctgct ttttgtgctg cgtaatctgc acctgctttt
    30661 tcaaattcag ctttttgttc atctgtagga tcgatgatta catcgccttc gatcccgttg
    30721 acagccaaaa tatcgccttc tttgacttta gcagtaattt cttttgtccc aacgattgct
    30781 ggaatttcta aagaacgtgc catgatagca gaatgtgaag tacgtccacc aatgtctgtt
    30841 acaaacgctt ttacaaaatt gcgatctaat tgtgctgtat cacttggagt caagtcatgt
    30901 gcaacaacga ccacttcttc gttgatcatt gatggatttg gtaaagtaac gcctaataag
    30961 tgcgctaaaa tacgtttagc aacgtcacga atatctgcag cacgctcttg catataagca
    31021 ttatcttcca ttgcttcgaa cataccgata tacatatccg ttacttcttt tagcgctgat
    31081 tcagcattta cgctgttgtc tttaatattt tgcttgattt gacctaccat ttctggatct
    31141 gaaagaacca tcaaatgggc atcaaatact tgcgcttctg cttcacctaa gctttgcgct
    31201 gctttttcac gaatttgctg caattcttca gtagatttag ctaatgcagc atccaaacga
    31261 gcttcttcag ccgcagtatc ttctactgtt gccttactga atgttaaatc cggttgaact
    31321 agcaggtaag cttttgcaac ggctacaccg tcactagcgg caatcccttt tagcatttca
    31381 accattattc agataagcct tctttcttca ttgtttcaac gattgctgcc attgcatcag
    31441 cttcgtcagc accttctgca gtaatagtaa catcagaacc ttgacctaca cctaaagaca
    31501 taacgcccat gattgatttc aagtttactg atttaccttt atactctaag tttacatcag
    31561 agttaaattt gcttgctgtt tgtactaata gtgtagctgg acgagcgtgg atcccagttt
    31621 ctgctactac gtgaaattct ttcttttcca tattaatcgg tctcctttaa gtgagtgttg
    31681 tttttatgtg agggttttct tttttagtaa ttcagagaat cctaacaaat gccacccttt
    31741 catctgtaaa gattaacatt ttttttcgtt agatacaaca gttttcattc aatttcagat
    31801 aaaagtattc aaattattcc ggattctttt tcatcctttt cttcacattt tataacaaac
    31861 gtgtttattt cctttttctt gtcgctttct cttctcctga cgtttatcat tctttccttt
    31921 gaccctcttt gttttcatat gaaacgaatt acttgcttcc ctttttattt ttagtataat
    31981 aagagtcaaa ggtcaggaat ggtcaataga gatttcacct tttctacctc ataaatctct
    32041 taaaaaacta ggggttattc ttttacctca tgaaaaacga gatgtaaaat atggatgtac
    32101 ccatttttgt gaaaggacgt gaatgattca tgctttgtca aaactgcggc aagaatgagg
    32161 caacgattca cttgtatgcc actgtcaatg gtcagcggac acaactcgat tactgtcaga
    32221 gctgttatca aaaaatcaaa aatcaacaaa atggaggtct gactaatatg gcccaaaatg
    32281 atccatttgg atttggcagt ttggacgatc ttttccgttc catgtctcgc caaatgcaac
    32341 agcaaggaaa ttatgagcaa acaccaccaa cacaatttgg cggaaacaac aacttcaatg
    32401 gtggtcagcc acctcaaggc aatacagaag gtttattggg agaatacgga atcaatatca
    32461 cagaacaagc acgacaaggt gatatcgatc ctgtgatcgg acgcgatgac gaaatcaaac
    32521 gagtcattga aattttaaat cgccgaacca agaacaatcc tgtcttgatt ggagaaccag
    32581 gtgttggtaa aacagcagtt gttgaaggtt tagcacaaaa aatcgtagat ggtgatgtac
    32641 cgcaaaaatt aatggacaaa gaagtcattc gtttagatgt cgtatcttta gtccaaggta
    32701 ctggtatacg aggacaattt gaagaacgta tgcaaaaatt gattgaagag atccgtcaag
    32761 cagaaaatgt gatcctattc atagatgaag ttcacgaaat cgtcggtgct ggttctgctg
    32821 gtgatggaaa tatggatgct ggtaatattt taaaaccagc tttagctcga ggagaattgc
    32881 aaatggttgg tgctactacc ttaaatgagt accgcatcat cgaaaaagac gctgctttag
    32941 aacgtcgtat gcaacctgta cgggtagatg aaccaactgt cgaagaaacg atcagtattt
    33001 tgaaaggttt gcaaaaacgt tatgaagact atcatcatgt aaaatatact gatgaggcca
    33061 tcgaagcagc cgcaacttta tccaatcgtt acgtccaaga tcgcttcttg ccggataaag
    33121 ccatcgattt gctagatgaa acaggttcta agaaaaactt gacgatccaa atcgttgatc
    33181 ctaaaacaat cgagaaaaaa ctgcaagaag cagaagaaca aaaagtttta gcttctcgag
    33241 aagaagattt cgaaaaagca gcttattatc gcgatcagat caataaactt caaaaaatga
    33301 aagaacgtca gctcactgaa gaggaaacac ctgtgattac cgaaaaagat atggaaaaaa
    33361 tcgttgaaca acggacaggt attccagtgg gtgaattaaa agaaaaagaa cagacacaac
    33421 tgaaaaactt agcagatgac ttgaaagctc atgtcatcgg acaagataac gctgtagatc
    33481 gagtagcgaa agcgattcgc cgaaaccgtg ttggattaaa caaacagaat cgtccaatcg
    33541 gctccttctt atttgttgga ccaactggtg taggtaaaac agagcttgcg aaacaacttg
    33601 cctacgaact attcggttca caagattcaa tgattcgttt cgatatgtct gaatatatgg
    33661 aaaaacatag tgtctctaaa ttgattggtt ctcctccagg ttatgtcgga tacgaagaag
    33721 ctggtcaatt aacagaaaaa gtacggagaa atccttacag tcttgtcttg ttagatgaag
    33781 tagaaaaagc tcatccagat gttttgcata tgttcttgca aatcttagat gatggtcgat
    33841 tgacagatgc acaaggaaga acggtcagct ttaaagatac gatcatcatt atgacaagta
    33901 atgccggaac tggtaaagta gaagcaaacg ttggttttgg tgcggcacgc gaaggtgtta
    33961 ctcgttctgt attgaaccaa ttaaacaact actttacacc agaatttctg aaccgttttg
    34021 acgggatcat cgaattcagt gcattatcaa aagaaaactt gatgacaatc gtcacactaa
    34081 tgctagacga tgtcaaccaa atgctggcag cacagcaact tcacatcgaa gttcctacga
    34141 atgtcaaaga aaaattagtt gacttaggct atgatccatc tatgggtgct cgtcctcttc
    34201 gacgcacgat ccaagaacaa atcgaagatg gtatcgctga attttatctt gatcaccctt
    34261 ctatccatga gttgaaagca aaactggata aagatggaaa aatcatcgtt acttctaaac
    34321 cagaacgttt ggctaaagaa tcagccgaag aaacggctga ataaatccag ttttatagcg
    34381 tgagacggta tttctttgag aaatgccgtc tcattctttt ttcagaaaag tcaaaatact
    34441 gttcatttta taaaaaagaa attataataa gagaaacaag caattaaaaa tacataaaag
    34501 aggtgagcgc catgaaattg gtaaatgtaa caaacagcca ttcccgtctt gtattaaacc
    34561 agcttgaaaa cactgatgcc catctagtca aggtctacac agccggaaat accactattg
    34621 tctacactga agcccctgaa cataatgaaa ttctgctgat caatgacaaa cgtaaaatcc
    34681 agccgaaaga aatcgaagat gctaaaaatt atttcttaga taaaatcaaa gacgccgttt
    34741 accacgagga tgaaatcaaa gtaatcgaat tggcaggcct tgttgaaatc tcgattccaa
    34801 aacatgaatt aggcgctaca ttagccgtgt aaacaaattt tgaacataat aattaaagag
    34861 cgtgggacaa aagtaaagaa cacttttgtc tcacgctcta aacacgtata aacggcgaaa
    34921 gcagaagcaa ctccttcgga aataagctga aattcacgaa aattgctttt caaatttttg
    34981 tgaattccag cttatttctt ggagttaaac gcttctgtct cagcctcttt ttctttctag
    35041 ctttttgatt tttctctttt cttttgtact tttctctttt cattttcagt tctacgcgtg
    35101 tctttatttt taactttttc ctctttatgg atgattgatc gtactttttg tgcaatttgc
    35161 tcgtttttgt cccagtcttt tacatctaat aatcgacata atcgtaacat ctcttctttt
    35221 ttctgttttt ttctttcacg tctttgccat tctgtttcca tgtgtcacac tctcctttct
    35281 ttaatcatat gtacgcaaaa taatttttat atttttttga ttagacattt ctatgatttt
    35341 aatatagtaa aaagagccgt cacggaaaat ccgtcacggc tctaaacaaa atatgagagg
    35401 ctgtttttgt ttggctattc ttattacttg atgcagggca gcttgtgtca agacttcttt
    35461 acatcaaact ttttaatttt acattgggat atttatccgc aaaccagcgt tctgcaaatt
    35521 ggttctcgaa taagaacaac ggttggtcga aacggtcacg agccaagata ttgcgacttg
    35581 aactcatctt ctcatccaaa tcttctggat cgatccagcg agcgattttg tgtcccattg
    35641 gagacatgat tacttcggcg ttatactcat ttaacatacg atattggaat acttcgaatt
    35701 gtagctgacc aacagctcca atgatatatt cttccgttag atatgtttta taaagctgaa
    35761 tcgctccttc ttgaactaat tgatagattc ctttgtggaa ggatttttgt ttcatgacat
    35821 tttttgcagt cactttcata aacagctctg gagtaaatga agggagttct tcataagcaa
    35881 cttttagttt tccttcgaac aatgtatccc caatctgata attgcctgta tcatatacac
    35941 cgatgatatc gcctgctact gctttttcga cgttttctct cgaatctgcc ataaattgtg
    36001 tgacattgct taatttcagt tttttatttg tacgttccaa ccagacatcc attcctcgtt
    36061 cgaatgttcc agaacagatt cggacaaaag cgattcggtc acgatgggct ggattcatat
    36121 ttgcttggat cttgaatacg aaacctgaaa attcttcctc ataaggactt accatctgat
    36181 catcttgtgt tttgtgtcca taaggagctg gtgccaattc aacgaaagtc tctaaaaaag
    36241 tctgtacacc gaagttagtc aatgcagaac caaaaaatac tggtgtctga tcgccacgag
    36301 caattttttc ttgattaaat gcatctccag cctctttgat tagttctact tcttctaaga
    36361 cttgttcata taaggaattt tcagttagag gatgattttc gggaagcagt ccatcttcat
    36421 ccaattctac taatcgttca tcctgataat tttccggacg ataaaactct acacgattat
    36481 ggtagatatc ataaagacct tctagccctt tacccatacc gattggccaa ttcatcggat
    36541 aagattcgat atctaatagc tcttctaatt cttctaacaa ttccaatggt tcacgaccat
    36601 cacgatctag tttgttgata aaagtaaata tcgggatgcc acgttttttt acaacttgga
    36661 ataatttctt tgtctgtgct tcgatccctt ttgcactatc gattaccatg accgcactat
    36721 ccacggccat caaagtacga taagtatctt cagaaaaatc ttcatgtcct ggtgtatcca
    36781 agatatttac tcgtttccct tgataatcaa actgcataac agaactagta acagaaatcc
    36841 cacgttgttt ctcgatttcc atccagtctg atttggcaaa attacctgtt tttttgcctt
    36901 ttacggtccc ggcttgacgg atagccccac caaacaatag taattgttct gtaatcgttg
    36961 ttttcccagc atccggatgg gaaatgatcg caaaagttct gcgattgtcc acttgttgct
    37021 ttaattttgg attattcata acctaactcc atttcttcca ttcaattatt tatattttct
    37081 ctaggcacag attgtccgat ctcttaaaga aagacaattt catatagggc tgtgacaaaa
    37141 gccgtgtagc agtctctttt tccgagtaaa cggcgttcta gagacagcct atcagcaata
    37201 agcccaccca agccaaaaat acgaaaagct gtttttgctt ggctgctctt attacttaat
    37261 gcaggatagc tttttcacaa cctttgtcaa ctactacaac tgaatcagta tagcatggat
    37321 tcagcgaatg aaaaagccac caaaccataa gagtcaaata tttttttgtc agctgactaa
    37381 aagaaaagac gtgttgcttg tttattcaag caacacgtct ttcataacca agtttccaat
    37441 tatcttcttg gctcgtcatc ttcgaattct tttctgaagt atcttcgttc ttcttcaggt
    37501 tccgcttctg tttcttccgg atcatgaaaa atgacacgga ttttaaggat tcgagaacct
    37561 tccatttctt cagaaaccag cgtaatatta tcgacatcaa acgataactt ttcgccttca
    37621 tccggaatca tgcctaatgc tgtaatcaaa taaccagcca tcgtatctac atcactcata
    37681 tgcagatcac tgtcaaaagc ttcattgaac tcatcaatca gcatacgtcc ttggatgata
    37741 tattcgtgtt catcgatttt ttcataaaga ttctcaactt catctgattc atcatcgatt
    37801 tctccgacga tttcttctaa aaggtcttct agtgttgcta acccaacgac gccgccatat
    37861 tcatctaata aaatagccat ctgattctgc gtctttttca tttcatataa cagatcatca
    37921 ataaagaccg tttcaggaac gaataaaggc tcctgcatga ttttcttgat atctaaatta
    37981 tcaaagccga atttatgggc agcttttagt aaattctttg tatgaaggat tccgactact
    38041 ttatctttgt cttcgttata aacagggatt cttgaatagt tttcggataa aacttcattc
    38101 acattttctt ccacagtgtc gttgatatca atcataaatg catccgttcg tgggaccatg
    38161 acttctcggg caactttcgt atcaagagaa aaaacccctt ggagcatttc taattcttca
    38221 ttattcaata cgccttcgtt ttcaagcata taacgcattt catctctggt catttttgaa
    38281 tcttcatcat caaaagtcat cggcgtgata cgcgccaata aacttgtgga agcagaaagc
    38341 agccagacga agggcttagc tactacacca atcaagcgta tcagccctga agtgaacttt
    38401 gcgacttctt ctgatttatt taaagcaata cgtttagggt acaattctcc aaaaacaatc
    38461 gatacatagg tcaaaatagc cagcacaatg atatttgcaa tattttttgc cgctgtgccg
    38521 ccacctaata ccggagctaa ccgttccgat aaagtattag ctagtgaagc accagacaaa
    38581 atgttgacca aagtgattcc tacttggatt gtcgatagga aattatttgg attctgcaat
    38641 atttttaaaa gcttttgaga tttcacatct ccttcatcag ctttttgctc gattcgattc
    38701 ttattgatcg aaacaacagc aatttcggaa gctgctaaaa aagcattgat caatgtcaaa
    38761 acgatcaata ataaaatctg cgctaacagc gactgactct cagggtcagc attcataatc
    38821 gcattctctc cttataaaat taatttttag tttacgatag aaaaaaacgt ggcactatag
    38881 ccacgttaaa atggtatcac aaagcaattt tctttgcaat aaaaacaagt gtactaaaac
    38941 tacctacgag gtcgtagcaa ttttcttctt gttttcattt tgcttgtctt ctttgacgat
    39001 tttgattata taggaaatga tcgtcttgca gattgcgtaa aaaggaacac ctaagaagat
    39061 accaagcaat ccggcaatat ttccagctac tagaagaacg ataatgatcg ttaatggatg
    39121 gattttcagt gatttcccaa ttacattagg ataaatgata ttcccatcca actgttggac
    39181 gattagcaca accacacaac aaagtaatgc ttttaccgga tcattgaaga ctgttactag
    39241 aaatgcaggt gctagtccta gataaggtcc taaatatgga attaggtttg tcaatcccgc
    39301 aatgacccca aataagaaag cataatccac accaattacc atatatccta aaaatgtgaa
    39361 ggtaccaacg aataaacatt cgattgcttg tccgctaatg taattagcca atgttttatt
    39421 cagttgcccc aataactcca caatttggtt tcttcttttt tctgggaaaa actgttggat
    39481 gtttggcact agtttttctc cgtctttcaa catatagaac aagatgaatg gtgctgtaat
    39541 gattactacg gttgccgaag cgatcgttcc aacgatcgaa cctaaactat tggatactcc
    39601 actaaggaat tgctgaatga ttgtcccgta agaaatgtct aattgctcaa aatatttatt
    39661 gatatctact tccttgaaca aaggcaattc ggctacctct tttaaccagc tttctacctg
    39721 tttgatgaaa tcaggcatat tagatgccaa tgaggaaatt tgctgtacta gacttgggat
    39781 gacactaagt aaaatccaaa taattgctgc aactaaaagg agcagcacaa ttgcgacagc
    39841 ccatattctt ttgatttttg ctttcatcaa cagcttgaca ataggattca ataaatagta
    39901 aagaaagcct gcaatcagta caggtgcaaa taacgtggaa aaaaatgtcc cgatcggttc
    39961 gaaaataaag tttatttctg atgaaatcat gatcaatgca gctatgatca ataattcaag
    40021 cgaccaaaac attagtttag actgtctaag tttttcaaac aatagatttc ctcctttctt
    40081 tcttactagt atatattata ttcgttaaaa attcttttat tcttttttta tt
//
LOCUS       DACDUU010000019        39532 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.019, whole genome shotgun sequence.
ACCESSION   DACDUU010000019 DACDUU010000000
VERSION     DACDUU010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.019"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..520)
                     /locus_tag="IWL30_001020"
     CDS             complement(<1..520)
                     /locus_tag="IWL30_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9705804.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNVASIVMSTIFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVA"
     gene            731..1177
                     /gene="nrdI"
                     /locus_tag="IWL30_001021"
     CDS             731..1177
                     /gene="nrdI"
                     /locus_tag="IWL30_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP9705805.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEYEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQRFGFPFLADYELRGTPSDVTRVYQILAEKNK"
     gene            complement(1247..3229)
                     /locus_tag="IWL30_001022"
     CDS             complement(1247..3229)
                     /locus_tag="IWL30_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9705806.1"
                     /translation="MAKNELTLEELAKQIYQGAGGMGNVESIVHCMTRVRMKVINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQEMVDLAGVKLGEKLPSASATTAS
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAGTWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALATGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKAVPQASA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVISSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAADK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(3243..4139)
                     /gene="murQ"
                     /locus_tag="IWL30_001023"
     CDS             complement(3243..4139)
                     /gene="murQ"
                     /locus_tag="IWL30_001023"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP9705807.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELPSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISEHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEAAQAKLAEANGFVKGTL"
     gene            complement(4142..5203)
                     /locus_tag="IWL30_001024"
     CDS             complement(4142..5203)
                     /locus_tag="IWL30_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP9705808.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQNRLIELGSEAKKNKLELMVDISGEALKRAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPNDIDELKEAAADFSQIEAWHNYYPRLETGLDKEWYQEK
                     NQWLKTQGFLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSEHVQEQFASFQKEQTVLLHTEPVDEVFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPVRNTAKRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(5415..5942)
                     /locus_tag="IWL30_001025"
     CDS             complement(5415..5942)
                     /locus_tag="IWL30_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9705809.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQALAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELISYE"
     gene            complement(6009..7511)
                     /gene="lsa"
                     /locus_tag="IWL30_001026"
     CDS             complement(6009..7511)
                     /gene="lsa"
                     /locus_tag="IWL30_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP9705810.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            complement(7769..8890)
                     /locus_tag="IWL30_001027"
     CDS             complement(7769..8890)
                     /locus_tag="IWL30_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9705811.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            9016..9957
                     /locus_tag="IWL30_001028"
     CDS             9016..9957
                     /locus_tag="IWL30_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP9705812.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(10100..10996)
                     /locus_tag="IWL30_001029"
     CDS             complement(10100..10996)
                     /locus_tag="IWL30_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP9705813.1"
                     /translation="MNKNDVLLIAKNKSLYFFPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAEKYKQEFTKR
                     GVTTNYFILPLRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYQFYHSENIFLLQERDLDELPEFLHTPFQPIPSELLEFFDAENWAKR
                     FFTTDSAIYEQYEYDLSSVKNK"
     gene            11278..12618
                     /locus_tag="IWL30_001030"
     CDS             11278..12618
                     /locus_tag="IWL30_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9705814.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGIILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHAADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFAAYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLIIFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLTLRKGTLW
                     DKGISFFALAVLWLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            12813..13256
                     /locus_tag="IWL30_001031"
     CDS             12813..13256
                     /locus_tag="IWL30_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP9705815.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINAMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILTSKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(13518..14936)
                     /gene="nhaC"
                     /locus_tag="IWL30_001032"
     CDS             complement(13518..14936)
                     /gene="nhaC"
                     /locus_tag="IWL30_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP9705816.1"
                     /translation="MEKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKKYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDILEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPKLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(15340..16563)
                     /locus_tag="IWL30_001033"
     CDS             complement(15340..16563)
                     /locus_tag="IWL30_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAP9705817.1"
                     /translation="MTTTLSTNQLKDLIQYRLPEYQALALAIHDHPEVSNYEFYSSAA
                     LIKQLEKEGFWVEKEVAGHRTGFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDEIGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDAALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAALSTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSEKGLASIAIAAELLANTALDLLREPKLLEKIKQE
                     HQEIVARKKQEGATF"
     gene            complement(16595..17434)
                     /locus_tag="IWL30_001034"
     CDS             complement(16595..17434)
                     /locus_tag="IWL30_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP9705818.1"
                     /translation="MKKNIRFAVITFVAIIWLAGCSQGKAATTEKKETTVKLGIIGED
                     TDVWDDVKDRLKKENIHLEYVKFTDYNQPNAALADGSIDLNSFQHQFFLDNYNEEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENKKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDEHARPYVNIIVARKEDEDNKTYQKIVDAYQTEETAKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(17451..18131)
                     /locus_tag="IWL30_001035"
     CDS             complement(17451..18131)
                     /locus_tag="IWL30_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9705819.1"
                     /translation="MTEFFKTYLPNVYLIPDEFIEATKQTLYMSFWTAFIGGIIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILVLVFLSQFISN
                     QLIKKVTH"
     gene            complement(18133..19206)
                     /locus_tag="IWL30_001036"
     CDS             complement(18133..19206)
                     /locus_tag="IWL30_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9705820.1"
                     /translation="MIELRDISVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEAGK
                     IIEKGSSIQIFSHPEEELTKDFIRTATHLDQALETISAHAAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRQKALDYLIKS
                     GVKVTILQKNELDNRIKVIEGGN"
     gene            complement(19856..20527)
                     /locus_tag="IWL30_001037"
     CDS             complement(19856..20527)
                     /locus_tag="IWL30_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9705821.1"
                     /translation="MKGAIFDLDGLLVDTERTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVQAAGSPEKVQEVRAKREEYFYQQLTSGAIQLKPYAKEILTDLKERHM
                     KLGIATTTVTKRATDILDHFQLADYFDTFTFGDEVSENKPSPVPYLTALERTGLEALS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYSVEEKEKLNLLAEGKDLRTVIEMLD
                     KKTEK"
     gene            complement(20615..23293)
                     /gene="mgtA"
                     /locus_tag="IWL30_001038"
     CDS             complement(20615..23293)
                     /gene="mgtA"
                     /locus_tag="IWL30_001038"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAP9705822.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASPNGLS
                     EEQIERSRETYGENAISYGKKTPFIIEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGEYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNDTLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEAAETLDTDTINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVAPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELITGEKISEMNDHELAQVAEEHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGKFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQQVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKIPFIQSNATFAMLVITTLGIIVGSVLPFTGFGAELGLM
                     PLPGNYWLWLFITVVAYLALVTAVKKIYIRRFQELL"
     gene            23953..24390
                     /gene="ndk"
                     /locus_tag="IWL30_001039"
     CDS             23953..24390
                     /gene="ndk"
                     /locus_tag="IWL30_001039"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAP9705823.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFATMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKGADAVPGTIR
                     GDYALPGTENIIHASDSRDAATKEIARFFPPFDTNKPSEKAAK"
     gene            complement(24412..25278)
                     /locus_tag="IWL30_001040"
     CDS             complement(24412..25278)
                     /locus_tag="IWL30_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9705824.1"
                     /translation="MYDGELIKKLRVNKKLTQTQLAEGICSKTSLVGIETNSVKKVSF
                     GTLKSFLERMNMTLAEYEWIRNQLDEPKKLKKKRRMLDRIQEDSFDPYKEIANNRRHY
                     KKTEDLYYLVLNLHMSKKTEVSETEFEFLRYECRIIEEYLMQVPEYGQFEVSILADFP
                     WIFSDRFIENNYLKIKKRMRRIAQLNNHEQYLFTFLTNLTLFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKSENHQEFFAVIEYTLGKNQRNSL
                     EKKLLAIAKPSS"
     gene            complement(25512..27590)
                     /locus_tag="IWL30_001041"
     CDS             complement(25512..27590)
                     /locus_tag="IWL30_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP9705825.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVSKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPATLGQTEGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTIL
                     YPVTGTNAEQ"
     gene            28083..29300
                     /locus_tag="IWL30_001042"
     CDS             28083..29300
                     /locus_tag="IWL30_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP9705826.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEENTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETREKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEASPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(29759..30382)
                     /locus_tag="IWL30_001043"
     CDS             complement(29759..30382)
                     /locus_tag="IWL30_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705827.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWEEYKLTANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLVVLLLTHDGDKVARLKNGKVVTAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     ASSQKNESTELSPSDSGSGQTITGNEENQASIITNESSEGQYQTYGQ"
     gene            complement(30538..31935)
                     /locus_tag="IWL30_001044"
     CDS             complement(30538..31935)
                     /locus_tag="IWL30_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9705828.1"
                     /translation="MKRYQFPKNFWWGSAASGPQTEGRISGDGKGENIFDYWYKNEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HHYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFQLFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKAYHEMDQSGVI
                     GIILNLTPSYPRDESNPEDVKAARIADAFFNRSFLDPAVKGEFPSELIERIKELDLMP
                     EIHPDDLTIIKENTVDLLGINYYQPRRIKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEKGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDRNGFEA"
     gene            complement(31957..32928)
                     /locus_tag="IWL30_001045"
     CDS             complement(31957..32928)
                     /locus_tag="IWL30_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9705829.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMEKISEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISNRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHAIDGLIVFLSEEEWSGFEMLTKMSIPVVALNCPGGPAGIDTIVVNDR
                     ESVAKVIAHHGGQGHKTIGFIAAAQTDYVARERLAGAKEAVQKDPSIHLEIFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            complement(32935..35235)
                     /locus_tag="IWL30_001046"
     CDS             complement(32935..35235)
                     /locus_tag="IWL30_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 3 glycosyl hydrolase"
                     /protein_id="HAP9705830.1"
                     /translation="MYDYPYQNPKLTIRQRVEDLLSRMNIAEKVGQVNQHLYGWECYE
                     KNGDKIELTEKLKEHVKWGGGLGALYGLFRSDPWSKTDYKNGIPAKESWKVANLVQEY
                     VLQHSRWKIPVLLVEECPHGHQGLDGISYPTNIGRGNTFNVALLEQSSRLMAKELAAK
                     GVHLALVSTLDLAKDPRWGRTEECFGEDPILSARFSEAVVRGFQGEIISEKVSFLDQT
                     VEEINKKKDQIGVVLKHCIAQGEALGGHNSGTVTIGRREFSDIYGPLLKSTRNAVGVM
                     AAYNDIDGIPCHINRALFEQTLRKEIGYQGIVMADGVALDRLSDVFTDKKTAAAYALA
                     AGIDLSLWDETYTKIAEAIDNQVVDEKLLDQAVRRVLSVKFLLGLFEQPFANDPKEYW
                     DHLMEESEHLNLETAKESITLLKNDGILPLDQQVKNIAVIGPNAHDVYHLLGDYSAPQ
                     TEDRLKKKIVQEIKTEFPQSKVSYAQGCEVRNQEDQEEKLLEAVTLAQNSDVIIAVLG
                     GSSARNFDMEFLRNGAVSSKGINMDSGENVDVASLSLGGYQEQLIEQLYQLGKPIITI
                     LVQGRPYDLMKIESFTNAIATAWFPGQEGGKAIAQMISGRNNPSGRLSLSYPRNSRQL
                     PVYYYQRAASKQENYYDLSGSPFYPFGHGLSYTTFEYSQMEVKTDIDKVIISIKVKNT
                     GSVSGKTSTLVYVRLIDGVVIQRTKLLKAFRKDELRSQEERLLVFELTSEDFSYMDID
                     DKRHYAKKAVIMVEQQEKEIKCNWQE"
     gene            complement(35237..36562)
                     /locus_tag="IWL30_001047"
     CDS             complement(35237..36562)
                     /locus_tag="IWL30_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9705831.1"
                     /translation="MKVVDRLSEKMLPIVSKMSSQRHLLAIRDSFIVTMPLVMAASVM
                     VLLNALIFSNATVQRILDLSPLAELASIVNNGTMSILAIIVCYNIGWNLATHYIQTGV
                     IDDPSFSPVHAGILSTAVMFILMSLSSTVTLMDGNTAEATGVFLQSLTSSSGLATAMI
                     AALVSTELFIKLAKMKSFKIRMPEGVPPAVAASFNSLVPEIVVILFFSVVVFTLNHTT
                     GLNVPQLIELAIQTPLKAFVLSVPGILFLQFFSDFLWVFGMHGSSILAPIRQAPLLQS
                     IQENMDAFSVGKEIPNIITEPFTNAFGLIGGGGCILPLVIAILWASKRQEQRSIAKFG
                     LTTCLFNITEPIMFGLPVVMNPIYMIPCAIIPSINLVIAYAATSLGIISKTVAAAPWI
                     TPPVIQSFIATGGDIRAAVLTVILIILDVFLFLPFVLAANKAKLAEGGY"
     gene            36793..37218
                     /locus_tag="IWL30_001048"
     CDS             36793..37218
                     /locus_tag="IWL30_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705832.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DITNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGADSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKEATVTFTKIGNYRLKDKDGNIYSL"
     gene            complement(37716..38417)
                     /locus_tag="IWL30_001049"
     CDS             complement(37716..38417)
                     /locus_tag="IWL30_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP9705833.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDTFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(38433..38873)
                     /locus_tag="IWL30_001050"
     CDS             complement(38433..38873)
                     /locus_tag="IWL30_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HAP9705834.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVLAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFEKLPVETYVGEYL"
     gene            complement(38873..39448)
                     /locus_tag="IWL30_001051"
     CDS             complement(38873..39448)
                     /locus_tag="IWL30_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAP9705835.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT        11386 a         8424 c         6852 g        12870 t
ORIGIN      
        1 tagctactac aataaataaa aataatggca ctaatttaca aaatgtgacg atagcattga
       61 caaagctagc attttcaact ccacgattca ctagccatgc atatccccat aagaagatcg
      121 tactcatcac gatcgatgcc acattttgac caccttcgaa tacggggaag aaataaccta
      181 atgcactcat caacaatgtt gcgaacgcca cgtttcctaa ccagcatgtg agccagtaac
      241 cccaaccgct gataaaacca gcgaaacggc caaaaccttc ttctgcgtaa ctaaagatcc
      301 cgccttttaa atctggacgt tttttattga tattattgat cgataaggag agcatcaaca
      361 ttgctgttcc tacgagtacc cagctgaaga tagctgggcc accagccgat ccggagatgt
      421 ccgtcataat accaaatacg ccacctccaa tcgctgaacc aatcactaag gcagcaagtg
      481 gaatcaaacc aattcctttt ttctgttgct ctgtatccat gtaagcatcc tccaaagtaa
      541 aaccgctttt atcaaaaagt ataacaaacc ttttttattt ttacttaatt aataaaaact
      601 ttaatagaaa tataattcta ccgacttttt tctcattata caagcaaagc gcctagtccc
      661 tttccaaatt acaccaccaa aaaaacttaa aaagatgcta tactaaaggg aaagaattgg
      721 gggatgatcc atgaacattt tatatatatc gatttcagga aatacgcgtg cttttgccaa
      781 acatctagca gaatatgctg aaaaaatgca tgcagaagat cctgtcaatc ctgaagttac
      841 tttaaaagaa atccatgaaa atagtgattt tacgaaagaa acagaaccat tttttacttt
      901 cgttcctact tatttagacg gcgggaatgg gttagacaac ggtgacacag aaatcttgac
      961 cgaaacgatg cgcgaatatc tggaatacga agacaatcat aaactttgcc ttggtgtagt
     1021 cggtagcggt aataaaaact tcaacaatca atattgtctg acagccaaac aatacgcaca
     1081 aagattcggc tttcctttct tggcagatta tgaattacgg ggaacaccat ctgatgtgac
     1141 acgcgtttat caaattttgg cagaaaaaaa taaataacac aaaagaagaa gctgaaaaat
     1201 aaatttcggc ttcttctttt tatgttctct attgtttcta ttcttattaa gctttgacat
     1261 ccatcacagg tgcttttgct ttcgctttac ctgttttttc caagacaaag ttttttacac
     1321 gatccatatt ggttaccaag acgatcatgg aggtttcttt gtctgctgct ttgattgctt
     1381 ctaagtcagc ttctgctaca agcgtatcag gtgtcacttg ctgtccttct gttactttca
     1441 catcgaatgg cgtaccgcct aagtcaactg tgttgatgcc catgtgaagc aagatttcta
     1501 agccatttgg tgtcgtaatg ccgatagcat gttttgttgg gaaaacagaa cttattactc
     1561 cagtaaccgg ggaatagata tgtccgtttt ctggttctac aaagtaacct tcacccatca
     1621 ttttttgtgc aaaaactggg tcacttgctt tatctaaaga aacaactgtt ccgtcagcaa
     1681 ctgatgataa gatagtcaca ctttcagctg aagcttgagg tactgctttt tcaggtactt
     1741 catttactgt ggctgtttcg gtcgctagtt cttctttttc taaacggtct ttaggaatac
     1801 cgaagaagaa tgtagcaacg aagccaccag cgtaagcagc caataaacca aggatatagc
     1861 ctaaccattt accatcagag atcaatggaa tcaaagcggc tcctgaaggt ccaatagcga
     1921 ttgctccgac atttccgatc attccgatca ctgccccacc gatacctcca ccgatacaag
     1981 cagtaataaa tggacgtcct agaggtaatg ttactccgta aatcaatggt tcgccgattc
     2041 ctaagattcc gactggtaaa gcccctttga tcatttctgt taattctttg tcttttttgc
     2101 agcggatcca taatgccaat gctgcaccca cttgtcctgc tccggccatc gcaaggattg
     2161 gaagcaataa ggtacgacca gtttcatcaa tcatttgtac atggattggc gtcaagatct
     2221 gatgtagtcc gaacatgacc attggcaaga ataatgtacc tagaacgaag ccggcaaatg
     2281 ctcctccaac accaagtacc cagttgatcc caccgaccat gccgtcagaa atgaaccctg
     2341 ctaaaggcat gatgaagaaa atttcgatca gaccgatggc aatcagggaa agagttggcg
     2401 taacgatgat atctaccgca tcaggtacta ctttatgcag ttttttctca acgattgaca
     2461 ataaccaaac tgcgaagata acacctaaga tcccgccttg tccagttgca agagcttcac
     2521 cggtaaacaa gtttttcagt ggtgcttctg gattcatccc tgtcagcatc acaacagccc
     2581 cgataactcc accaagcgtt ggcgttgcac caaaaacttg tgcagcattg atacctgtat
     2641 agataactag atagctaaac atcccgtttt tcaagatgtt catcacatcg atatattgtt
     2701 gccatgttcc tgcgtctagt gttccagctg tgactaagtt tgctaaaact gcggcaatcc
     2761 ctgccacgat cccagtgccg acaaatgcag ggatcattgg tacaaagata ttagagatgt
     2821 cttttaatac cgatttgatt ttagagggtt tttgtttcga tttttgtgcc gctttcattt
     2881 cttgtgcccg ttcgtttaca cggtcttttc cagatgcaga agaacttgct gtagttgcac
     2941 tagcagaagg aagtttttct cctaatttca cgcctgctaa gtcaaccatt tcctgcgcta
     3001 ctttgttcac tttcccaggt ccgatgatga cttggagctg ttcatcctct actacgccaa
     3061 gtactcctgg aatcgctttt aattccgaga catttacaag atcatcattg atgaccttca
     3121 tacgtacacg agtcatgcag tggacaatgc tttcaacatt gcccataccg cctgctcctt
     3181 gataaatttg ttttgccagt tcttccaaag tcaattcgtt ttttgccatg gtcttttccc
     3241 cctcataagg ttccttttac aaaaccgttt gcttcagcta attttgcttg agctgcctct
     3301 ttatcactat ttgtcaaaat catgacgata gccagtttga cattttgatc tgcttcctca
     3361 aagtactttt cagctgtttc gtagtcagct tctgtcgctt gcatgatgat tcgttttgag
     3421 cgttcaatta gtttttcatt tgttggtttg acgtcgacca tcaagttatt gaatacttta
     3481 ccgataccga tcatactgat agtagaaatc atattcaaaa tcagtttttg tgctgttcct
     3541 gacttcaatc gagtagagcc tgtcaaaaat tctggtcctg catcgacttc gattgggaaa
     3601 tccgcatgct cgctgatttc tgcttgttta ttgcaagaaa tcgttcctgt aacagcacca
     3661 atcgattttg cgtaatctaa tccaccaatc acatatggtg tacgaccgct agctgcgata
     3721 ccaataacta tatcattttt tgttaaattc agcttgatta gatcttcttg ccctaattca
     3781 taagaatctt cggctccttc gacggctaca gtcattgctt cttctccacc agcaattagg
     3841 cctatgacca tttccggttc tacaccgaat gtcggtacac attcagcagc atctaataca
     3901 ccaagtctac cactcgttcc agctcccata taaatcaagc gaccaccttt tttaaaggat
     3961 tcaatcgtgt tggcgatcac tggctcgata cttggcagtt ctttttcaac agcagcggcc
     4021 actttctgat cttctttgtt catgagttgg actgcttgag aaatactcat ttcatctaat
     4081 ccaaaagttt cttggtttct gcgttccgtt gttaggttct ctaaattgat tttgctcatt
     4141 tttatcttcc ttcttttctt ataaattgat agttcatacc agctttgatt tggttgatca
     4201 gcggtagttc ttctttgatg actttggcta ctacattcac tttttcatcc gctggcaaat
     4261 catatttagt taattggatc tcacccatat agcgcaaata cttttcgtta tctaaagtaa
     4321 tagatccttt catacgctta gctgtatttc ttactgggat tggcggaatc tctcgaaaac
     4381 gcgcatctgc gcttcgtatg acatctcttg catcatcttg ccgattcgta tgttttccta
     4441 atatatattc gtaaaaaact tcatctacag gttccgtatg gagcaagact gtctgttctt
     4501 tttggaaaga agcaaattgc tcctgaacgt gttctgacag acctgcatct ccgatataaa
     4561 caatatccgt gttacttgct aatagatcaa gagcacctgc cagaggatgg atccctcgat
     4621 gttcttccaa agttggtaat ccttgataca gtggacctct caactctgta tctcctggta
     4681 caaagccttg tacaaggaat ccttgagttt tgagccattg atttttttcc tgataccatt
     4741 ctttgtccaa ccctgtttca agacgtggat aataattatg ccaagcttca atctgtgaga
     4801 aatcagccgc tgcttctttc aactcatcga tatcattagg cgtgatcgtg ctggcattca
     4861 aactgatttt caattgatgt gaccagtcgg cgatctgctg attagaaata tgataatcca
     4921 ttcttaaccc tgttacacca attgctttca agggtgttag atcttctaat gaaaagccag
     4981 ctcgtttcaa tgcctctcct gaaatatcga ccatcaactc tagtttattc tttttcgctt
     5041 cgcttcctag ttcgatcaat cgattttgat aagccgaaac atcatcttcc ggtatatgca
     5101 tggaagtgaa aataccgaca aagccattgg atgccatttg gcggatatac tgtttcttct
     5161 cttctgacaa agaatcattc ataaatattg aaaagccaaa catattatcc ctctttttct
     5221 gtatccgtct tcatttctat gactagagta tatatctaaa gaaacaaaat ttcaatactt
     5281 ttgttaaaat aattaaattt tatttctaaa aattggattt ataatgccaa aagaattgac
     5341 acagccacaa aaaaggcact gctaagcaaa agcagtgcct tgaaacattt tatttaaata
     5401 acacaaaagt ctaattactc ataagaaatt aattctgtat tgtctaccat gaatgtaaag
     5461 aacccttcat taggcattgg atgaaaataa gcattgatca ctcttatcaa ggcattatga
     5521 cagactaaca agtagacatt ttcttcgtct tccatgcatt ctttcaataa aggaacgatc
     5581 cgtgcgtaca catctaagac cgactctcca tttgggaaac gtacagcaaa cgctaaacgc
     5641 gctttttgaa agttttcatc tttgcttggc agtccgtcat aatccccaaa atccatttct
     5701 accaatcgct catcagcggt catcggcaaa ttgagacgat caactactgc ttgtgctgta
     5761 tctcttgctc gttgaagagg ggaatggatg attttcgtga ttggaacagg caaatctact
     5821 accttttctg ctagtgcctg tgcctgcatc tgtccttttt cagtcagcgg cacatctgct
     5881 cgcccgcaaa ctaatccttg tgcgttccaa gttgtttctc cgtgtctcgt tacataaagc
     5941 atgtgattct ctccttcgtt gacaaagttc tccttaacca taccttgttg tctttgagat
     6001 tacaaggttt agattttctc aagagaaata atctcagtag atactttatc tagaaaggtt
     6061 tgatcatgtt ccactagtaa catagcaggt ttcacgttca aaatcagttg ttctaattgt
     6121 tcttgattga agacatccaa ataattcaat ggttcatccc atatatagag ctcagctggt
     6181 tgggataaag atttagccaa ttccactttt ttccgttgtc ccatactcat ctgttcgatt
     6241 ttattatgaa aaacatctct ttccatccca agctttcgga gattattcaa aaatgcttga
     6301 tagtctactc ggtttttctt cgcgaattcc gccaatgtcc ctcgattgtc ttcataattt
     6361 tggcgtacgt aactaatggt caaatgtttg ggctgggatt tttctcctac aaccttgccg
     6421 ttgaatgtcc ccagaagata atggataatg gatgactttc ccgcgccgtt aggacctgaa
     6481 attgccatcc gctgatgagg ttcgatcgta aaatgaactg gctcgaataa cagattttca
     6541 taccctaatt gaagattttc tacgcttaac agccgtttat gatgagacac ttggctattc
     6601 atcgtcaaag catcgatata ctcgatatct tttaataatt tttctttctc actgatctgg
     6661 gtttccatcc gattcacgat tgctttagat cgtttcatcg tccgtgcagc atcagcgcct
     6721 actgctcctt tattcactcg cctagattcc gtatcgatga atccgacttt tttctttgtt
     6781 ttgtcccctt ctcgggaacg agaccattct gctttttcag ctgctgtttt ctttagcctg
     6841 ctgacttctt ttttcaattt ttcattttta gctatttcaa attcatcacg aagttttttc
     6901 tgttcttcat agatagagaa attcccttga taaagttcca gttggctctt ttcgattgct
     6961 aaaacatggt ccactacttc atcgataaat ccccgatcat gactcaccac gatgaatcct
     7021 tgttttttct ttttcaagta agccgctact tgttttctgc cagagatatc caaatgattc
     7081 gttggttcat cgattaacgg gaaatgagtg tcatccacaa ataagagtgc cagtaggact
     7141 tttgtcttct ctcctcctga tagtgtgctg aattctctcc ataagatttc aggatctgtt
     7201 tgcatcagtt ggagctctct ttcaatttcc catatctcaa aatccataat ctcatttaac
     7261 acataatagg tcaaacgctc tttatctttt gtttgctgcg ggaaatagac aaacgcttgc
     7321 tgatggatca cttgcccttg ataagggagt ttgttttgca ggatattcag taaagttgtc
     7381 tttcctcgac cgtttcgtcc gataagtcct agtttccatt gtgtgtcaaa gtttagattg
     7441 gcttgttcaa ataataatgt gccttggctg tcatagccga atgtcagatt ttttatttcg
     7501 attttagaca ttattccatc acctcgtaaa aaaagaccag tcctattttc agaactggct
     7561 aaagaggatg caagggttgg ggcacacaaa aagacccata catcgaaaga accaagccaa
     7621 tcccgaaaat ctgcacactg ctcccttgtc tccacaaggt acagtctgaa tgcagctttc
     7681 atcaatcgac ttaatttttc aatgcacgga tccctcactt tgtcctatat ttgaaattac
     7741 gatagcataa agaaattgtt tttgtcaact acttcgggag aatgttgaaa aatctgctga
     7801 gtgtctgtcc taagatttct gttttttcta atgtttgcct gtgttccatt gcttcctcta
     7861 aatcgattgg tcctaattga atgcttaaga caatggggag aaaatcaaag agtccttcca
     7921 gctctcggtc tctacttccg caaatagcga ttattggttt tcgatatttt tttgcaagca
     7981 ctgctacgcc atatggaact tttccgtgga ttgtttgctg atcaatccgc ccttctcctg
     8041 taatgatgac atccgcttgt tcgatctgtt tttctaatcc agtgatttgg ctgaccaact
     8101 caaaaccaga gaccatcttc gctcctaaca aaaaaagggc tccgccaata ccgcctgctg
     8161 ctccgcttcc tgggatgtca tctaaatcca cctcccaaac atcttttatc tgctgagcaa
     8221 tcttttcagc ttgtttatcc aagaaagcta acgtcgtatc cgttcctcct ttttgtcttc
     8281 caaattgcca agcagctcct tgttgacctg tatatgggtt ggtcacgtct gcagcgatga
     8341 tcacttttac tttagaaaca agaacgttcc ttcgatccaa atgaagtttt ctagtagata
     8401 acaaaggatt tccttctgtg tacccttcta aagaagcacc caaagattgg agtaatccta
     8461 gaccaccatc gctacatccg cttcctccta aagtgacaat gatttgttcc acttcttgaa
     8521 gcagtgtatc tttgataagc tctcccaagg cataactaga agcaagtcgg atggtttgat
     8581 ccgttggttt atcaagtaaa tctaatccta atacttgtgc agattcaatg actgcggtat
     8641 tttttccttg ccagttcgtc aataaataac tgatttttcg ctgacgaaat aagagatcca
     8701 tcccatcaat cgtacgccag gttcccccga gcgcgtaata gattgcttct gcagtatttt
     8761 ctccgccatc tgcaatcggc acattgattt ttcttccttt acggtcttcc caaccattta
     8821 gaactgcctg atttaattca aatgaagaag cacttccttt aaatgaatcg attgccgcca
     8881 aaatatccat ctatatccct cactttcttc ttaagtaaga cacttttttt cacttttttc
     8941 aaacaaaaca atgctatttt cttctgtttt tctatatact aaaagggaac tcattgaata
     9001 aaggagagaa aaaacatgaa aatcgcaata gctggagccg gagcaatggg cagccgaatc
     9061 ggattaatgt tgcatcaaag cggaaatgag gtcgtcttga tcgatcgctg gcctgctcac
     9121 attgaagcca ttcgtacaaa tggattgatt gctgacttta atggcaaaga agtagttgca
     9181 aaactgccaa tctattcacc agaagaaatc atcgaaagca acaaacacgt ggatttgatc
     9241 gtagccttga caaaagcgaa tcaattagat gatatgtttc gttcgatcca atccattatt
     9301 actgagaata cttatgtttt gtgtttactg aatggattag gtcatgaaga tgtactggaa
     9361 aaatatgtac caaagaaaaa tatcttattt gggatcacga tgtggacagc tggtctagct
     9421 ggtcccggaa aagtcacatt attaggtgat ggagaaatcg aactagagaa cttggaacca
     9481 gaaggcgaag catttacaaa aaaagttgta gaagtctttc aagaagcaaa attgaatcca
     9541 atctatagtc ataatgttcg ttattccatt tggcgaaaag cttgtgtaaa cggaacatta
     9601 aatggattat gcacgatatt ggattgtaat attgctgaat taggtgcgca aaaagcggca
     9661 gagggcatgg ttcgaacgat tgtatctgag tttgcttcta tcgcagcaaa agaaggaatc
     9721 acattagatc aagaagaagt gtaccaacat attgcttcta cttatgatcc tgacaacatc
     9781 gggttgcatt acccatctat gtaccaagac ctgatcaaaa accatcgttt gactgaaatc
     9841 gactacatca atggtgccgt ttggcgaaaa ggacaaaaat atgatattgc tacaccatat
     9901 tgtgcctttt tgacccaatt agtccatgca aaagaaggaa tccttggcgc agaataaaag
     9961 gctctctgat gagaatcagc tggatcagca gctggttctc atttttttac ccccatgaaa
    10021 gttcaaaaag aaaaacacaa ttctcatgtt gccataagaa ttgcgcttgt ttattttcat
    10081 ctatgattgg ttggcacttt tatttatttt taacagagga aagatcgtat tcatattgtt
    10141 cataaatagc ggagtcagta gtgaaaaaac gttttgccca attttctgca tcaaaaaatt
    10201 ccaacagttc tgatgggatt ggttgaaatg gcgtatgcaa aaattccggt aattcatcta
    10261 aatcacgttc ttgaagcaaa aaaatatttt cagaatgata aaactgataa tgagctatgg
    10321 cttgattatc tgtcactagt ttcttttgaa aatacataga ttcaaacgtt cgcaaagtca
    10381 ctccttgctg cccttcccgc aacagttcta gaatccccct cgattccccg gtttttttca
    10441 gatattcttc ataagtaagg tatccaggtt gttcatttcc tcgaagcgga agaataaagt
    10501 aattggtcgt aacacctctt tttgtaaatt cctgcttata tttttctgcc cgttcttttc
    10561 tgccgttatt tgttgcacca aaaaatagat ccgtctttgg ttcgctagaa gaaaaatcca
    10621 tatgtttcga ataaaaggaa ctattatgtt ttagtccaag ttctttcgct tctaaaaaat
    10681 caaaatgata aatctcatcg attgccggtt catcttgtgc ctgcttcagc atcgcctcgt
    10741 gttctgcttt aaagtggttc caaaaaaata acacgatttt tgcattggta aatggacgca
    10801 cgcgtcgagc catctccata tcaaatccat aatctaaaaa aacgatcagc tgataatttg
    10861 ccaactgttg tcgagctgat ttaatgaatt ccgactgtag taaatctttt ccggaccaaa
    10921 catctaaaga gcggatggaa ggaaaaaaat aaagtgattt atttttcgca attagcaata
    10981 cgtcgttttt gttcatctgt tccgcctcac tttctctgtc actttttcct atgatagcct
    11041 gtttaaatag taaaaagcca gtaaaacgct tcctattatt tttataaaaa tttaacattt
    11101 ctatgaaaat acgatgaaat aaatcattct tccattaaaa tatgcccaaa agttttctga
    11161 atagttgtaa ttatctgaaa atttgttatc attagtcatt attattatta ctatttattc
    11221 ttactattta ttcttactat gcattttgat tgtgaattta aaagaaagag gttatttatg
    11281 gaagataaaa aattgaaaaa acaacttagc tcccgacata tcaccatgct agctttaggt
    11341 ggagcgattg gtgcaggtct atttaaagga agtggcgaag cgattggaat cgccggccct
    11401 tctgtattga tcgctttttt gattggcggc ataattttat atatcgtgat gaaaggtctg
    11461 ggaaaaattg ttttaagtgg tggtgatacg caccacggat tatctggatt gattcgtcca
    11521 tatctaggtg ctcatgctgc cgattttacc gattgggtct actggtccat gtggattatc
    11581 aatatcattg ctgaagctgt tgcagctgct tcctttttac agttgtggtt cccccacatc
    11641 ccagcttggg tatttgtttt tatgttggct gttttaacta ctatcatcaa tctttattcg
    11701 gtacgattat tcgcagagac agaatattgg ctggcctttg ctaaaatcag tgtcattatc
    11761 ctattgatca tttttgctgc ttatcttgtc ggtcagcaaa tgttgggtac tggtgttttc
    11821 cctacccttc aacaattaac tgatcatggt ggttttactc cccacggtat gaagggaatc
    11881 atcagttctt tgcttgtagt tatctattct tacggcggtt ctgagttgat tgctatcaca
    11941 gtaagcgaag cagatgatcc taaacgtgcg attcctaaag caatcaaagg cgtcatggga
    12001 agaatcattt ccttttacat tattccttta tttttgcttt tgattatttt tccttggaat
    12061 actttagcag gcacgaacgt tagtcctttt gtgatggtat ttgaaaaaat gaatattcca
    12121 tttgcggcag atatcgtcaa tttcgtgatt gttcttgctc tgttctcgtc tatcaattca
    12181 ggtgtatatg cttcttcgag aattttgttc ttccgcttga aagatcggaa aggttcctct
    12241 agaaaactag ctgttttaaa caagcatcaa gtaccgcaac gtgctgtgct gttttgtacc
    12301 agtgttcttt atctaggtgt ggctctttct tattttgtcg gtgacaaatt atttggctat
    12361 ctggcaggtt ctctttccta tacagtatta ttgatctgga tcattatcag tgctgcgtcc
    12421 tttgtactga cactaaggaa aggaacgtta tgggacaaag gaatcagctt ttttgcatta
    12481 gcagtcttat ggttgatttt ccttggtatc ctattcacga actccattgg ggtaactgta
    12541 ttgaccggtt tgctttacct gttcatttat ttcagttatc aaaaaaagaa tgatgctttc
    12601 gtcttgacga atgaatgaaa ccaatagaga attttattct atttatattg aatatctgga
    12661 gctattgaaa aaaaggagct gcgacaaaaa ttgttgcagt tcttttttcc gaataaacgg
    12721 tgttcaaaaa ccatctgtgc agattaattg attgttctcc acgctcatga catgatagaa
    12781 gaaaggaatg aagaaaaagg agtttgtttt aaatgaaaaa gtattggaag tttttgttga
    12841 tgattggtgt ttcgacggta gtcatgtttg gtatgatgta tttgaatgtt tatgcgcttg
    12901 atcatatctt cttcagtcgc acacgtgtat tcatggcgtt gatgatgggt gcagtcatgg
    12961 cagtcatcat gttgttgttc atgtggaaaa tgtatgaaaa caaaaagata aatgctatga
    13021 ttttagccgc tagtcttctt gtttttgccg gttccttatt tatggtacga agccaaacaa
    13081 ctgtcaacga tacggcgtgg atgaaggcga tgatccctca tcactctatt gctatcttga
    13141 caagtaaacg agctgattta aaagatcctg aagtaaaaaa attagcggaa gaaatcattc
    13201 aggcacaaga agaagaaatc gacaaaatga aaaaaatgat caaagaaatg gaatgaccat
    13261 tatccatcca aataaaaaaa gaggatgttc cagaagtgat caacttcaag aaataagaag
    13321 aagctacttc tggaacaccg tgaatcagag gttgtgaaaa agctgccctg catcaagtaa
    13381 taagaacagc caaacaaaaa tagcttctca tatttttcgt ttggttaggc ttattaccgc
    13441 aaatgcaagc ttttgaacac cgtttattcg taaaagggag tgtgacaaaa gtcttgtcac
    13501 actccctttt tacgttttta ttgttctaat tgcgctgtct tgcttactgc ttcttgcggt
    13561 ttcacaaatt tctttgagat cccccaacct gtaaaggcac tgaccatcga aaacacaggt
    13621 gaaagcaaac tgaaaaacac gaatggcaag taagtgagtg taggaatgcc aaatgtactt
    13681 gctacgaaac ttccggcgat tccccaaggc actaagtagt tgattactgt tccaccatct
    13741 tctagtgttc tccctaatac agttggatca aatcctgctt tttcgtaagt ttctttaaat
    13801 gcattaccag gcaggataac agatagaaat tgttctccaa taaagatatt tactccgatg
    13861 cttgtcatca atgttgtaaa aatcaatttt ggggtgttcg tcatctttct tgatactcgt
    13921 tccataacgg ttgagatcaa cccaaattcg atcaacaaac cacctaatga caatgttaga
    13981 ataatcaatg acacagttgg catcatgctt tcgattcctc cacggctaag tagtgaatcg
    14041 atttgttggt ttccagtatt tgaaacaaat cccgattcga tgacccctgc caacttcgtt
    14101 aaggaagttt gtgggttttg aataaagatc attcctactg ctacagcaat attcaacaaa
    14161 atcgtcaaaa ttgccggtac ctttttccat gcacacaaga gcattaaaaa tagaggaatc
    14221 agtgaccaga tcgaaatagt aaagtttgct tctaaaatat cagtcacttc acgaatagat
    14281 gaaagacttg cggctttact tgtgtgtcct aaaatcatga ataaaaagaa agaaacaaca
    14341 aatgccggaa cagttgacca catcatatgc ttgatgtgtt tgaacaaatc ggcatcaaca
    14401 atagctgctg ctaaatttgt tgattcggat aatggagaca tcttgtctcc aaaaacggca
    14461 cctgaaacaa tggctccaac aacaagggct ggatttagtc ctagtgttgt cccaataccg
    14521 aaaaatgcaa tcccaatcgt tgacataaca gtaaaggcac tccctacagc gctacctacg
    14581 attgcacaaa caacaaatac agaagggacg aaccactgga cactaatcaa tttaaatcca
    14641 atgaccatga gggttggaat aattcctgct tgtatccata cagcaatcaa tgaaccgact
    14701 aaaataaaaa taaagattgg aatgatcccc ggtttgatgc cattgatgat cccttcgtgc
    14761 agccaatctg tcgggtattt ttttatcact gcgtacaaca tgaccattcc gatagctgcc
    14821 aaaatcgtga tattaggtga aacctttaat ccgacaacgc caatcgcaat gcctaaaaca
    14881 atgaccatca atacaagtgt tgcttctaaa aaacttggtg ttcctttttt ttccatgata
    14941 atctctcctt ttttagttgt tcttatgttc taaaaaagtt aagatatcgg attactccgc
    15001 cacaattcat ccgcgattcc tcctttataa aaataaaaag acctgctttc aactaaggac
    15061 gaatgactcc gcggtaccac cttaattaaa aacagatctg tttttcactt tcaaacgatt
    15121 attcaaatgt atggactttc catgtaattc gtcaattagg cttgatcggt tcacagcgac
    15181 caccgacttt ctgaacaaaa cacctaacca ctactgcgtg aaaagttgac ttgtatgaag
    15241 tttaccttcc ataagatcat ttgtcaatga aaaagaaaat attttttcat ttttctgaaa
    15301 attattttta taactttcgc gcacaacttt atctttttac tagaaagtag cgccctcttg
    15361 tttttttctt gctactattt cttgatgttc ttgtttgatt ttttctaata gcttgggttc
    15421 tctcaataaa tctagagctg tattggcaag taattccgct gcaatcgcaa tagaagcaag
    15481 tcctttttca cttttagcag cagctttgaa ttcttctgta tgtccggcaa tatatgtctc
    15541 agagattgct acagtaggct gtatcgttgg aataacttgg ctaacatttc ctacatcaga
    15601 agaacctaga cttgttcttt gttccgtttc tatttcttca tcaggaaccc ctgcttcttt
    15661 tgcatgttgg aagaataagt catcgaaaga aggcgttacg atgacatcat cgactgcatt
    15721 ttggaacaat ccaaactcaa aagtcgtacc cgtgcttaat gcagcacctc gtacgatatt
    15781 ttccactttt tcatatactt tgtctaatgt ttgtcgattt cctgcacgta aatagaaacg
    15841 tccagcagcg tagtctggta tgacattggc cgccacgcct ccatctgtaa tgattccatg
    15901 gatattgaca tcttttggca attgcaagcg tagagcgttg attccgttaa acacttggat
    15961 caaggcttcc aaagcattta ctcctttttc aggtgctgct gctgcatgag aagcgacacc
    16021 gtaaaatttg atatcgactg gatcatttgc taaagattct gtcgttttcc cataacgaaa
    16081 agctggatgg acacataatg ctgcatcgac atcctcaaaa aaaccttccc gaacgaagct
    16141 tcccttagct gatccatttt cgcctccttc ttcccccgga gtaccataca cacgaatttc
    16201 tccgccaatc tcatcaatga attgtttcac tatgctggct gctaatacgg agtaagtacc
    16261 aaataaatta tgtccgcatg catgaccaat acctggtaaa gcatcaaatt ctgccaaaaa
    16321 ggcaattgtc ggacctggtt tattggaaat ataacgtgca tcaaagcctg ttcggtgccc
    16381 agctacttct ttttctaccc agaacccttc tttttccaat tgtttgatta aagctgctga
    16441 ggaataaaat tcataattac ttacttcagg atggtcgtgg atagctagtg caagtgcttg
    16501 atattctggt aaacggtatt gaatcagatc cttcaattga tttgttgaga gtgtggttgt
    16561 cataaaaatt tcctcctatg ctagtcgttt tttcttattt tctcccgaat gtttcccacg
    16621 caggtacgct tgaacctttt gatgtttttt cgattacttt ggctgtttct tctgtttgat
    16681 aggcatctac tattttttga tacgttttat tgtcttcatc ttcttttctt gcaacgatga
    16741 tattgacata aggacgtgca tgctcatcga ctggttctaa gaagattgca tcagaagcgg
    16801 gattaaatcc agcatctaca gctacaccgc tattgatgac tgaaatatct acatctgaca
    16861 aagcacgtgc tgtttgggaa gcatctaact cagaaatgtc tagatttttt ttattctcgg
    16921 taatatcaga aacggtaggc gcttggcctt ttgccttgtc cactttgatc aatccagcag
    16981 tttgcaataa cagaagagct ctgccgccat tcgtgacatc attagggatt gcaacttttg
    17041 ctccatcttt gatatcagcc acatctttta acgtattgga ataaattccc aacggtgcat
    17101 taaccgtatt cccaatcgat actaaatcag ttccgtgttc ttcattgtaa ttgtctaaaa
    17161 agaattgatg ttggaaggaa ttcaaatcga tcgatccatc tgctaaagcc gcatttggct
    17221 gattgtaatc tgtaaatttc acatattcca aatggatgtt ttcctttttc aaacggtctt
    17281 tcacatcgtc ccatacatct gtatcttccc caatgatccc tagttttacg gtcgtttctt
    17341 ttttttcagt tgtagctgct tttccttggc tgcatcctgc caaccaaata attgctacaa
    17401 atgtaatgac tgcaaaacga atattttttt tcattttttc tcctccattt tcaatgtgtg
    17461 acttttttaa tcagttgatt actgataaat tgactcaaaa aaactaagac cagaatcaat
    17521 aatgtagcga caatcgttac atctgtttgg aatcgattat atcctctcga tatagcaaga
    17581 ttccctaatc ctccggcacc tactgctcca gccatggtag tcagtccgat cacattgatg
    17641 atcgtcaacg ctgagacccg aatgatacca ggtaaccctt ctaacaaata aacacgtaaa
    17701 atgatgccga ttgggctcgt gcccatcgct tctgctgctt cgatcacccc aggatcaact
    17761 tctagcagtg cattctcgat ttgacgggca aaaaacggga caacgccaaa aatcaaaggg
    17821 actaaagctg ctttggcacc aatcgtcgtt ccaactaaga aacgagtagt caaagctaaa
    17881 agagaaagca agataatgaa agggatggaa cgaatgatat tgatcaactt atcaagtatt
    17941 tcgaacagca gcctgttagc tagtaatcca tttggacgtg tcactactaa cgtcacacct
    18001 aagatgattc cgatgatccc accaatgaat gccgtccaaa aagacatata tagtgtttgc
    18061 tttgtcgctt cgatgaattc atccggaatc aaatagacat tcggcaaata tgttttaaaa
    18121 aattcagtca ttttagtttc ctccttcgat gactttgata cggttatcta attcattttt
    18181 ttgtaaaata gttactttta caccagattt aatcaaataa tccaatgctt tttgccgctg
    18241 tttggtttct ccagctaaag taacgatcaa acttcctaag ggtgtttctt gtaatagctc
    18301 tacattaccg tataagatat tcgctgttac ttggtacttg ctgtaaagat gagcgattag
    18361 aggttcgttc gtctggttgc cgatataact taattccacc aaccatttat ttgcaatctg
    18421 atcggcaaaa gcagcatggg cactgattgt ttccaatgct tggtcaaggt gtgtcgctgt
    18481 ccgaataaaa tctttagtca attcttcttc aggatgactg aaaatctgga tgctgctacc
    18541 tttttcaatg attttccccg cttccataac cgctaccttg ttgcagattt ctttgacaac
    18601 ttgcatctca tgagtgatca ggacgattgt tagacctagc tgacgattta gtttttttaa
    18661 cagtgcaagg atctgaaggg tcgttttcgg atcaagtgca ctagttgctt catcgcataa
    18721 caacacttta ggttcatttg ccagagctcg tgcgattgcc actcgttgtt tttgcccccc
    18781 cgacagttgt ttaggatacg cagatgcttt ttcttccaga ccgacaagtt ccagtagttc
    18841 attcactttt tgacgacgtt cctgttttga ttttccagaa tactttagag aaaaatctac
    18901 gttgtcaaaa atcgttcgac tttccattag attgaaatgc tgaaagatca taccgatggc
    18961 ctttctttct tttcgcaatt cttttggaga gagctggctt aaattcgtct gattgacgat
    19021 gacttctcct gctgttggtt tctgcaacaa attcatcacg cggactaacg tactctttcc
    19081 tgcacctgag taaccgacga tcccataaac atcccctttt tcaatcgtta aatctacaga
    19141 atctactgcc tgtatctctt tttctttttg tttgaaacat acagaaatat ctcgcaattc
    19201 aatcattaac attcctcctt gtctataaaa aaagcatcct caagaaaatg aaaatagtca
    19261 ttttcttgag gacgcctttt tgcgtgttac cacctcatgt tcaagctgtg ttcacacaca
    19321 actcctcttc aagtaccatc atactctagc aagatatcgg tcgcagccgt caccgcctta
    19381 catctatgct cgggatgaat tgctcgatgg ccattttcca atagtcgttc cttgcttctt
    19441 ctcagctacc gaagctctct gtcaaaggtt cttctatcgt acttaccatt tcaatgcaaa
    19501 taatattcaa aaaaaaaatc cttttactaa cataaactat gtcagtaaaa ggacgacgta
    19561 tcgtggtacc accttttttc ggaagtttcc ttccctcaag cccaacatct caaaaagaga
    19621 gattggcgtt cgttttaacg gtcgaacatt tccgaatttg cctacttatc gacaaattat
    19681 tctccaaggc cattttcagc ttttcttact tgactcttca caccagccag agcctctctg
    19741 caaagtttcc aagcttactt tccttctcaa cgaaatcaat attttgttgt tgggaataat
    19801 ctacaggagg aaaactgaaa agtcaagcat gaaatccaaa ttattttttc tatatttatt
    19861 tttctgtttt tttatctaac atttcaataa cagttcgaag atcttttcct tccgcaagaa
    19921 ggttcaattt ttccttctct tcgacgctat aatttcgaac aaaactggaa tcgggaatca
    19981 aaacaacgcc tagtcctgca cgtgaagcgg ctgtcgctcc tactaaagaa tcttccactg
    20041 caaaagctga aagagcttct aatcctgttc gttccaaagc tgtcagataa gggactggtg
    20101 aaggcttgtt ctccgatact tcatctccga acgtaaacgt atcaaagtaa tcggctaatt
    20161 gaaagtgatc caagatatct gttgctcttt tcgtaacagt tgtcgtagca atccctagtt
    20221 tcatatgacg ttcttttaag tctgtcaata tttcttttgc atagggtttc aattggatag
    20281 ctccacttgt cagctgttga taaaaatatt cttcacgctt tgctcggact tcctgcactt
    20341 tttcaggact gcctgctgcc tgcactaaaa tatcaaaact ttgtttccag ttttttccgc
    20401 cccaagcgtc aacgatttct ttcgggatat gaatatcaaa ttgctggaat ccccaaagcc
    20461 aaccatcccg atacgttctt tccgtatcca ctaacaaacc atctaaatca aatattgcac
    20521 ctttcatcta tacattaatc ctttccaaaa caatacacct agtttagcat gactgatcca
    20581 aaatgaacag aagcatagag cccagccgca tcacttataa gagttcttgg aatcgacgga
    20641 tatagatttt cttcacagct gtcacaagcg ccagataagc gacgactgtt ataaacaacc
    20701 ataaccagta attgccaggg agcggcatca agcctaactc agcaccaaat cctgtgaatg
    20761 gaaggactga accgacaatg attcctaatg tagtgataac cagcatggca aatgttgcat
    20821 tgctttggat aaatggtatt ttcggtgtac gcaatgcatg gagtactaat gtttgtgtcc
    20881 acagggattc gacaaaccat cctgcatgga ataatgcgat aaaaatcact tgttgtgagc
    20941 tatccaatgt atggaaactt ccaccaacta cagcaggaca aataacaaaa tacatcaaca
    21001 agtaagtagt gatatcaaaa atcgaacttg ttggaccaaa ataaaccatg aattttccaa
    21061 tactagatgc attccatttc tttggctcat ccaaatattc tttatccatc cgatcccatg
    21121 gtaaggaaat gcaagaaacg tcatagatca gattcaaaaa tagaagttgc aaaggcaaca
    21181 ttggtaaaaa tggtaagaac gtactggcaa tcagtacgga aaacatattg ccaaaattag
    21241 aacttgctgt tgccttgata tatttcatga tgtttccaaa agtttctctt ccagaaagaa
    21301 ggcctctttc caaaatcgtc aaatcttttt ctaaaaggat cacgtctgct gattcttttg
    21361 cgatatccac agctgtatcc acggaaatac cgatatcagc cactttcatt gctggagcat
    21421 cattgatacc atctcctaaa aatccgacag tatgcccatt tttacgtaaa gctgtcgtca
    21481 atcgtgcttt ttgctgagga ttcaatttta cgaatacttc gtgttcttca gctacttgcg
    21541 ctaattcatg atcattcatt tccgagattt tctcaccagt aatcaattca ttgatttcta
    21601 ggcctacttg acggcacaca gaccgtgtga ctaattcatt gtccccagtc agaactttta
    21661 cagctacacc atgttctttt aaagctttca acgcttgttt tgttgtttct tttggtggat
    21721 caaggaaagc aagataacca atcagaacca tgtctgactc atctttgact gaaaattctc
    21781 caacaacgct tggattggtc ttttgtgcga caccaatcac tcgtaagccg tcttcattaa
    21841 gtgattgtac ttttgcaagc accgcttctt ttcgttcttt tgttaatggt gcgacagtac
    21901 caccaagatc aataaattta gaaatgttca acatttcttc aatcgcgcct tttgtgatca
    21961 tctgtgtttt gccagccgga tcagcgacta caacactcat acggcgacgt tcaaaatcaa
    22021 aaggaatttc atctactttt tgataattga ttgtatctgt atctaaagtt tctgctgctt
    22081 catcaatgat agccacatct aacaaatttt tcagccctgt ttgatagtag ctgtttagat
    22141 aagcatggcg caaaacacga tcatcttcat ttccttcaca atccatatga tattcaagaa
    22201 tgattttgtc ttgtgtcaat gttcccgttt tatccgtaca taacacatcg atcgcaccaa
    22261 aattctggat agcatttaaa tttttgatga ctgttccttt tttggccatc acacttgctc
    22321 ctttgaccaa attcgtcgta acgatcattg gaagcatttc aggcgttaaa ccaactgcca
    22381 cagataatcc aaaaagaaaa gcttctaacc aatctccttt tgtaagccca ttgataacga
    22441 taactgctgg agccatcaat gacataaatt tgatcagcag catggatgtt ttgtggatgc
    22501 ctaattcaaa acttgattgg atgggttttt cagatagcgt ttctgctaca tgcccaaata
    22561 gtgtgtcatt tccgacagaa acaatgattc cttccgcgct tccactgatg acattgcttc
    22621 ccataaagac aagattttca taacttgttt cactttgctg atcgtcttca catactttcg
    22681 catgcttttc tactggatag ctttcccctg tcaatgctgc ttgagaaatg aatagatcct
    22741 ttgcggacaa taaacgcata tccgctggta tcatatctcc tgctgaaagc cgaacaagat
    22801 ctccacagac gatttcttcc ataggaatct ctgtatactc gccatttcgt ttgacagctg
    22861 ccgttacttt cacaagtgat ttcagttttt cagctgcttg attagaacgc acagactgga
    22921 tcaacgtcat cgtcccactg acgataacca tagtgaagat aatggctaca ccaaagagat
    22981 ctttatctcc tggagcggct aatacgtatt ccgtaatgaa tgaaataatt cctaatgcaa
    23041 tcaacactaa tgtaaagggt gtgatgtatg ctttcaatat ttctataatg aatggtgttt
    23101 ttttgccata tgaaatcgca ttttcaccat aagtttctct tgaacgctcg atttgttctt
    23161 ctgacaatcc atttggtgat gcatggaaca attcaaataa ttcttgcttc gtttttccag
    23221 caaaagctgc atagattttt tgtgtatctt tctttttttc catctcgatc tctcctttgt
    23281 gtccaaaaga cagctgttct cgctgttttt ggtcttttgc tattcatcgg atcgatagag
    23341 aaaatgctga aaggggctga gaacagatag ataatagccc tggcctttcc tctaaatcct
    23401 tgtttttcga atgactgtca tccatttgtt ctcatctcct ttactaagta attttaattc
    23461 atgacttcca atcatttttc gaatagttat tttcccaact cgctcacctc cctttttgtt
    23521 ttttcaccta tgggatttgc ggcaacaaaa aaacctctcg ccaaatactc agcgaaaggt
    23581 ttgaaaaagc aaatcaaaaa aagcttctca gatctcatcg ttgagttttg acactgtaca
    23641 acgtaaaaag cagatatctg cttttagcta ccttatgaaa agccttatct cgacaattcc
    23701 tgttctaccc attggcgtct ctcgacgttt ttgggcagta gcctatgttt gtataggagc
    23761 ctcacctaac aaatcctatt cggttttccc tcgttaatat aacaactaaa aaagaggatg
    23821 tcaaatcaat tttccatgtt tttcatactt ttttaaaata cacgcgttat atttcctgtc
    23881 tcacgcagtt ttatttgtct cattttccac ctcccgctat aatagaatta tagtaggaaa
    23941 cgaggtgctg ttatggaacg aacacttgtt attatcaagc cagacggcgt aagacgccat
    24001 ttggtcggaa gcatcatcca acgttttgaa gcaaagggac tggccattgc tgaaatgaaa
    24061 tttgcaacga tgacacctga actagcaaaa gaacattatc agcacttgac tgaacgtagt
    24121 ttttttgatg agcttatcga ttacatgact tctggtccgg tcgtttatct tgtgctggtc
    24181 ggcgaggaag tgatcgatat cgtccgtaag atggtcggag caacaaaagg ggcggatgcc
    24241 gttccaggta cgatccgagg agattatgca ctgccgggga ctgaaaacat catccatgct
    24301 tctgattccc gtgatgctgc aacaaaagag atcgcccgct ttttcccgcc attcgataca
    24361 aacaaaccgt ctgaaaaagc ggcgaagtga tacaaatcct aaactgttgg tctaactgct
    24421 gggtttagca atggccagca gttttttctc taaagaattc ctttgattct tccctaatgt
    24481 atattcgatc actgcgaaaa actcttggtg attttccgat ttttcttccc ctaaagcagc
    24541 agaaatcagt cgttggtaat aatccgccat caatgtttca taaatcactg tacttctttc
    24601 tttatgcgct aaactccgtt tcatatcttc attcacgcta cgtgcctttt ttaagcgacc
    24661 attttctata taaaacaaag tcaagttcgt caaaaaagta aagagatact gctcgtgatt
    24721 attcagttgc gcgatccgtc gcattctttt tttgattttc aaataattat tctcaataaa
    24781 acgatcactg aatatccaag gaaaatctgc tagaatagat acttcaaatt gcccatactc
    24841 tggtacttgc atcaaatatt cttcaatgat acgacattca taacgcaaaa attcaaattc
    24901 cgtctcagat acttctgttt ttttggacat gtgcagattc aaaaccaaat agtacagatc
    24961 ttctgttttc ttatagtgcc tgcggttatt agcaatttct ttgtaaggat caaagctgtc
    25021 ttcttgaatc cgatctagca ttcgtctttt tttctttaat ttttttggct catcaagctg
    25081 atttcgtatc cattcatatt cggcaagtgt catgttcatt cgctctaaaa atgatttcaa
    25141 tgttccaaac gatacttttt tcactgaatt tgtctcgatc ccgactagcg aagttttaga
    25201 acagattcct tccgctaatt gggtctgtgt cagctttttg ttgactcgaa gcttcttaat
    25261 caattctcca tcatacataa tagttctcct tttttaattg aaattaaccc ttggcttttc
    25321 cagaatgctt gccaataaga ggaatgatta tttcaattta aattttccgc attcaataaa
    25381 tagatacgtt aaaaataaaa aatacacgaa aaaaataaaa aaatgttttt ctcataaaaa
    25441 ataccaacct tcaagcatga gactgctgtc taatccctga aggttggttt gattgtttat
    25501 tgatcatttt tttactgctc cgcattggtt cctgttactg gatataggat agtcgttgtt
    25561 ttattgccgt tttttgaaaa gacactggtc gaatgttcac ctttttgctt ttcgattgct
    25621 tcattacgtt ccgcttcgtt tttatagtca tattttttcg gatcaatcgt ctcaaatccg
    25681 tttggcacat aaaaccgcaa gagattttcc tgattcagct tatctgaaag acttaacgca
    25741 gtcttgactt tttcctgatc tttggagatt tcctcgtcga ttcctgctgc tttcgtatcg
    25801 atgatttctc ctgtctgatt attgtaagct tttcctccta ggacggtgta tttgggcgtc
    25861 acgaagttcc catttcgaaa agcaactact tgatcgtgct gagaagataa taagtctgta
    25921 ccaaaatgaa gataatcttt atcatcgatt cccaatagat gtagtaatgt cggcaaaaca
    25981 tcgatttctc caccatattc atgattgact gtccctttag tgtaaccagg catatggatc
    26041 atcaatggca ctcgttgcat ctgtgcgttg tcaaattcat cccatgtatc tggatcttta
    26101 ccaagagcac ttgctaaatc tttattatct gtattcgaga tcccaaaatg atccccgtaa
    26161 atcacaaaga ttgaattttg gtaaatacca gattttttca aatacgtaaa aaattgttcc
    26221 aatgattgat ccagataatg ggccgttcga acataattat ctactgtgct attgccagta
    26281 ttcaaagatg gaaagtcgaa attcttgtca tccgtataat aaggcgtgtg gttggttacg
    26341 gaaagaaatt tcgtgtaaaa cggctgttgt agatgttcaa gatattgggc agattctctg
    26401 aataaatctt tatctaatat cccataacct aacgtttcat cggattcatc aaaataagat
    26461 cgatcaaaga aattgtcata ccctaaattt ttatatacat gatctctatt ccagaaacta
    26521 gctacattcc catggaaaac agcacttgta tagccttctg tttgaccaag ggtcgctggt
    26581 gctgcttgaa agacattatc tgacccaagt tctgtgaata atgagccttg aggtaaacca
    26641 aatgtgccag tttccaacat gttttcagca tcacttgtct ttccctgtcc tacttcatga
    26701 aagaaattgt caaaactgat cgttgcttga ttctggaaaa tactatttaa aaacggcgtg
    26761 acttcttgtc cgtctacctt catattgatc aaaaactgtt gaaaactttc taaatgaagg
    26821 acaatcacat tttttccttt agcaatccca aatgtttcag gatttggctc tgcataatgt
    26881 ttctttgtat agtctaaaac ttcatcaatt ccattactgc ttgcatgcgc gcgaacactg
    26941 ctagtcattc ctgttttgat tccgtcataa atagtgtacg catcgattcc tagatatttg
    27001 acaatatagg aacggtcaaa tgtccgctgt agcaattggg gacggttggc ttcgctcaac
    27061 gctaaatttc ctgaaaaaat caatcctgaa agacaggtaa cagcgatagc catcggtttg
    27121 ctgacagggt tgcttttgat aggtattttc ttgaccttca accaaaccag tagcccaatg
    27181 aacacagcga tatccaacca atagatgaca tcatggggtt tcatcaaaga aaaggagctt
    27241 cctgacaacc cttgtgacac tttcgaaaag cctaatacag atttgactgt aatgaaatct
    27301 gtgaattccc gataaaaaat aatattcaag tacaacaaaa gcgtatttaa aatatcaatg
    27361 atcaaaagaa tggctaacgc tggctttggc tttttaatat acaaagaaag gccaaaaaac
    27421 aataaggtag tcgcaatcgg gttgatccac aagataaagt actggatcgt accttccaca
    27481 cctaatgaaa aatctgcata gtaggcaatg atcgtcttca gccagaaaaa gaaaacagct
    27541 gtcaaaacga ttgctaaacg attttggcac aattttttaa gttgtgtcac gtatatcact
    27601 tctctctatt cacatttttt cttattttgc tcctgaaaaa tgaaaatttc ctaaaaaagt
    27661 tgttatctta agattttttt attctttcta aagattattc cattcatttt tcacagcata
    27721 aaatcgtttc ggtgtaagcg gttaaaatag taaggagata aaaatcttgt ggtgaccgat
    27781 ttactctata gtattcaaaa gtagtttctc ggtattcgta caaaaaaaac atactaagat
    27841 tagaggggaa catcattgac gaatgatgtt ctcctctatt ttctattttt agattatagt
    27901 gaagagtgaa agaaagttcg aacaaagcct tgaggactac gagtccctat caaaaaccga
    27961 acttctttac tgtcatttta gaatcaggtt tacgtatgtt ggggccatga gcccgtgtat
    28021 aggaaattgg aatcgaaatg aagcagtaaa gttctttcaa cacttaaaaa ggaggaaaac
    28081 aaatgcctta cattcttgca ttagatgttt caatgggaca cagctacagt gtcctataca
    28141 aagacgacac ctgcttattt gaagacgaga tcgaacacac tcaaagagga tttcatgcat
    28201 tactcgaaga gatccagcac ttgcctgaac gtccattgat tgtttttgaa gcaacgggta
    28261 tttattccag accagtcgaa aagttctttc aagatcatca cttttcttac tgtttgttaa
    28321 acccgttaga agcgaaaaaa cagatggaag aaaacacctt acgcagttgg aaaacagata
    28381 aatcggatgc ccatcggctc gcacagactc atcttaaaaa tcaacgtcaa ccaaaagaag
    28441 tacaaagcaa tttttactta gagatgcgcg atttagcccg tttttatcaa gaaattgaga
    28501 agaaaatcac tcgactgcgc atggatttac acaactgcct tcagttgact tttccagagc
    28561 ttgaacagtt cttttcaaac aggctgaccc cttacgcatt gacattgatc cgtctttttc
    28621 cccatccaga ttttgtattg gcatctactc ggacaaaaat aaaaaataaa ctgatcaacg
    28681 aaacacggga aaaaatctcc gcgaatcgtg ccgagcaaaa ggcagatcaa atcatccact
    28741 atgcacagtg tgcctatcca gctgttgaaa aagacagtat tcactgtcaa aaaacgatct
    28801 attacgccga acttctccaa gatttactcg aacaaaaaaa agcactggcg acacaaatga
    28861 tcaaaaaagc cgaagcctct ccttgctttt tgttgtacca aacgttccct gggattggcg
    28921 cactcacagc cgctttattg ctcggtgaat taggagatat cacacgattt aaaacccata
    28981 aacaattgaa tgcctttgtc ggaatcgaca tccgacggta tcattcaggt aagtatactg
    29041 gccaagaccg catcaataag cgtggtaatc ccaaagcacg gaaaattatt ttcttcacca
    29101 ttcgaaatat gattcgccag caacgcgcag ctcctaatca tattgtcgat tattactaca
    29161 aattaaaaaa gcaacctatc cctaagaaag ataaggttgc cacagttgct tgtatgaaca
    29221 agcttctgaa atgtatgcac gctatggtta gggcacatac agagtacgac tatgcgtacg
    29281 cggtctcagt ggaccattaa ctaaaccact tacagtatac cacttctttg cccagatcga
    29341 aaatttttta cgaacggggc tttatttagt gtgccttgat tttgtcgctt tcgtttacga
    29401 gctatttttc ttgcaccttt tacttgacta atcgtaggaa agaggctgtg acaaaattct
    29461 tgtcgcagct ccctatttct tgatgcagga cagctttttc acaacctctc cactaaacgg
    29521 tgttcaatca gctgaccttc ggtattagct caaaaaacgg caaaatatga ggagctattt
    29581 tcccgttttt ttctctaata ctcaggtcaa accgctgatt tcacaacctc tgattcacgg
    29641 tgttccagaa gtagcttctt cggaaataag ccgaaatttt acaaaaactt gagaagcact
    29701 tttcgtaaat ttcttcttat ttcttgaagc tgaccacttc tgtcacaact tctgtttttc
    29761 attgtccata ggtttgatat tgtccttctg atgactcatt cgtaatgatt gaggcttgat
    29821 tttcttcatt tcccgttatc gtttgtccac tgccagaatc tgaagggctt agttctgtcg
    29881 actcgttttt ttgagaagaa gcagttgttt tcttgacttg ttcgttggtc tttttccatt
    29941 ccgctagtcg atcggccacc acttttttgt ccactttttc agaataagct gtaaccactt
    30001 ttccattttt caaacgtgct accttgtcgc catcatgagt caaaagcaaa acgactaaat
    30061 gtttgtcttt gaattgatat tgtctgagag aaatcgcact aagtacgtca tcgatattga
    30121 taaattgatt ttcttctttt ccttttggca aatagatgtc caattcacct ttttcgtaat
    30181 tggcagttaa tttatattcc tcccaccaat aagttgagaa ttcttttttg aaattatcta
    30241 ctgattcttt gcttgctacg attttttctg tttctttctg agcaagatct gctgtcattt
    30301 ttcctgaaaa tgctgcggat aaaataagca gtaagaaaaa accgaataac caaaagagaa
    30361 gaaacggtct ttttttcctc atgccttcct ccactcctat ttaattttga ctattctatt
    30421 atatacaaaa aatgaaatag aaagacaaaa aagttgaaaa agatcgtaaa taattgaact
    30481 tctttttcct gaagcggtaa gaacgggaat tttcatacat tctctcttgg ctattcttta
    30541 agcttcaaat ccatttctgt ctgatacatc tttgaaccaa tgacccgatt tcttgatcgt
    30601 tctttttcca tcttgtgcta aatctactga aataaaaccg taacgattct tataggcatt
    30661 ggtccacgac cagttatcca tgcatgtcca catatgataa cctaaacaat tggcaccttc
    30721 ttgaatggct tgatggacat atttcagatg agattgaaca aattcgatcc gataatcatc
    30781 gtggatcata ccatcttcct tgataaatcg tgcttcattt tctacaccca tgccgttttc
    30841 agaaataaaa cagtcgatat tcccataatg atctcgtaca ttgatcaaaa tatcatagat
    30901 tcctttttca tagatctccc atccgcgata aggattcatt ttacgaccag gcatttcata
    30961 attgtcgaag taatcctcag gcataggacc gtttttagta tcgattggtg tttcttttgc
    31021 ttttattcgg cgtggttggt aataattaat tcccaaaaga tctactgtgt tttctttaat
    31081 gatcgtcaaa tcatctggat gaatctccgg cattagatcg agttctttta ttctctcaat
    31141 caattcactg ggaaactcgc cttttacagc tggatcaagg aatgaacgat tgaagaacgc
    31201 atctgctatt cgtgctgctt taacatcttc gggatttgat tcgtctcttg gataactcgg
    31261 tgtcaaattt aagatgattc cgatcacacc gctttgatcc atttcatggt aagctttgat
    31321 ggctaatgct gaagccaacg cttcatgata accaacttgg attgcctttt tcatatctga
    31381 ttcttccgga taatgccatc gatacaaata gcctgcctcg actggaacga ttggttcatt
    31441 atgggtaaac cattttttta ctttcgagcc aaacaattga aaacaacttt ttgcgtattt
    31501 ttcataggct tcaaccgttt ctcggttcaa ccatccgcct ttttcttgca gatacatcgg
    31561 catatcgaaa tgatacagat taacaaaggg ttcgataccg ttttcaatca actcatcaaa
    31621 atagtgatga tagaaacgaa cggcttcttc gttgattgtt ccagattcat caggtatcaa
    31681 acggctccat tggatagagg ttcgaaatga attatgccca gtctgtttca tcaactgtac
    31741 atcctcttga tacttcgtat aaacttgcga ggttttttca ggtcctactt gatcaaagaa
    31801 cttttgcggt tcgtttttgt accaataatc aaaaatattc tctccttttc catcgcctga
    31861 aattcttcct tctgtttgtg gaccacttgc tgcagagccc caccaaaaat tttttggaaa
    31921 ttgatatctt ttcatcaaag cacctctttt tcttttttag ttgattccat gtatgatatt
    31981 ttccatcaca atcacttttt cattttcttc ctcttctttt ttgattcgtt tcaacagctg
    32041 ttctgctgct cgttccccga ttttatctgt tggtggcgtg atcgtgattt ttggaaggat
    32101 actcagatcg tttttatcta aataatcata gtgactgaac gcaatatcct tcccgattct
    32161 gacaccttct tgattgaaac attcgatggc accaatcgac atgttgtaat tgctgacaaa
    32221 aatagcagtt gccccttttt ctaataatgc ttttgccccg taatagccgc tttttctaga
    32281 atagtctcct gtaaagatct ctaaatggat agacggatcc ttttgtaccg cttctttggc
    32341 tccagctaaa cgttctcttg ctacgtaatc tgtttgagcg gcagcaataa atcctattgt
    32401 tttatgtcct tgtccgccat gatgagcaat cacttttgct acactttctc gatcattcac
    32461 tactattgta tcaatacctg ctggcccacc cggacaattc aatgcgacaa ctgggatact
    32521 catttttgtc agcatttcaa atccagacca ctcttcttct gataagaaga cgatcaatcc
    32581 atcgatcgca tgcttcatca ggtattcgat tttttgttcg attttttctg gctgatcggc
    32641 aaacccgctt aataataagc tatacccatt ttcttcagct actctttcga tccctgaaac
    32701 aacctccgaa ccaaatcggt tagatatccc gtttatcaac agtccaatag agaaacttcg
    32761 attattcttc aatcctttag caatgatgtt ttctttatag tccaacgctg aaatcgcttc
    32821 tgatattttt tccatattct tttccttcag ctgctgaccg tttaaatagc gggaaactgt
    32881 tcccactgaa acaccagcca tcttagccac gtcacggatc gatgccatac attttcactc
    32941 ctgccagtta catttgattt ctttttcttg ttgttcgacc atgatgaccg ctttctttgc
    33001 ataatgccgc ttgtcatcaa tatccatata actaaagtct tcagaagtga gttcaaaaac
    33061 aagcagtctt tcctcttgag accgcaattc atctttacga aaagctttca ataatttcgt
    33121 tctttggatg acaacgccgt caattaatcg gacatagaca agagtacttg ttttacctga
    33181 aacacttcct gtatttttaa ctttaattga tattattact ttgtcaatat ccgttttgac
    33241 ttccatctga ctgtattcaa atgttgtata gcttagacca tgtccaaaag gataaaatgg
    33301 acttccgctt aaatcataat aattctcttg tttagaagca gcacgctgat agtaataaac
    33361 aggtagttgc cgactatttc taggataaga caaactcaat cgtccgcttg ggttatttct
    33421 tccagaaatc atctgcgcaa tggcttttcc accctcctgt ccaggaaacc atgctgttgc
    33481 aatggcattc gtaaatgact cgattttcat caaatcgtag ggtcttcctt gcacaagtat
    33541 cgtgatgatt ggcttgccta gttgatacaa ttgttcaatc agctgttctt ggtatccacc
    33601 taaagaaaga gaagcaacat ctacattttc tccagaatcc atgttgatcc ctttcgatga
    33661 aactgccccg ttacgaagaa attccatatc aaagttccgc gcacttgatc cacctagaac
    33721 tgcaatgatc acatctgagt tttgagcaag ggtcaccgct tctaataatt tttcttcctg
    33781 atcctcctga tttcttactt cacatccttg tgcataagaa acctttgatt ggggaaactc
    33841 tgtttttatc tcttgtacta tctttttttt caaacggtct tctgtttgtg gtgcagagta
    33901 gtctcccaac aagtggtaaa catcgtgagc atttggacct ataacagcaa tgttttttac
    33961 ttgttgatcc aacggcaata taccgtcatt ttttagtaaa gtgatacttt cttttgctgt
    34021 ctctaaattt aagtgctctg attcctccat gagatgatcc caatactcct ttggatcgtt
    34081 tgcaaatggc tgttcaaata aacctaataa aaatttgaca gataaaactc ggcgaactgc
    34141 ttgatctagt agcttttcat ctacgacttg attatcaatg gcttctgcaa tcttggtata
    34201 ggtctcatcc cacaaactga gatcgattcc tgccgctaat gcatatgctg cagcagtctt
    34261 tttatctgta aaaacatcgg ataaacgatc caaagccaca ccgtctgcca taacgattcc
    34321 ttgataaccg atctcttttc ttaatgtttg ctcaaaaagc gctcgattga tatgacaagg
    34381 tatcccgtcg atgtcattat aagcagccat gacacctact gcatttcttg tactcttcaa
    34441 taatggtccg taaatatcgg agaactctct gcgtccaatc gttactgtgc ctgaattatg
    34501 accgcctaaa gcttctcctt gtgcgataca gtgctttaac acaacaccga tctgatcctt
    34561 cttcttattg atttcctcta ctgtctgatc aagaaaagaa actttttctg aaataatctc
    34621 tccttggaac cctcgaacga ccgcttcaga aaaacgagct gagagaatcg gatcttcccc
    34681 aaagcactct tctgtccttc cccaacgagg atcttttgcc agatccaaag tcgacaccag
    34741 cgctaaatga acacctttag cagccagttc ttttgccatc aatcgactcg attgttctaa
    34801 taatgctaca ttgaaagtat ttccacggcc gatattcgtt ggataagaaa tcccatccaa
    34861 gccttgatga ccatgtggac attcttctac aagcaaaact ggaattttcc atcgcgaatg
    34921 ctgtagtaca tattcttgga ctaggttagc caccttccag ctttcttttg caggaatccc
    34981 attcttataa tctgttttag accacggatc agagcgaaac agaccgtata aggcacccag
    35041 accgcctccc catttaacat gttcttttaa cttttctgtt aattcgatct tgtctccgtt
    35101 tttttcataa cattcccaac catacaaatg ctggtttacc tgtcctactt tttcagcaat
    35161 attcatcctc gaaagtaaat cttctactct ttggcggatg gttaactttg ggttttgata
    35221 cgggtaatcg tacaccttaa tatcctcctt ctgccaattt tgccttatta gcggctaata
    35281 caaatggcag gaataaaaat acatctagaa tgataagtat tactgttaag actgctgctc
    35341 gtatatcgcc tcctgtagca ataaaagatt gtataaccgg tggagtgatc caaggtgcag
    35401 cagcaactgt tttactgatg attccaagac ttgtggctgc gtaggcaata accaagttaa
    35461 tagatggtat aatagcgcat ggaatcatat atattggatt catgactact ggtaatccaa
    35521 acataattgg ttccgtaata ttaaataaac aagtagtcag accaaattta gcgattgatc
    35581 gctgttcttg acgtttagaa gcccacaaga tagcaataac taacggtaaa atacacccgc
    35641 cacctccgat caatccaaat gcattggtaa agggctccgt aataatatta ggaatttctt
    35701 ttcctactga aaaagcatcc atgttttcct gaatagactg taacaaaggt gcttgccgta
    35761 taggagccaa aatactcgaa ccatgcatac caaacaccca taagaaatca gagaaaaact
    35821 gtaaaaataa gatgcctggg acggataaaa caaaagcttt caatggtgtc tgaatcgcta
    35881 attcaattaa ttgcggaaca ttcaaaccag tcgtatggtt taaagtaaaa acgacaactg
    35941 aaaaaaataa aatgacaact atttctggta ctaaagaatt aaatgacgca gctactgccg
    36001 gtggaactcc ttctggcatc ctgattttaa aactcttcat tttcgctaat ttgataaata
    36061 attctgtact gacaagggca gcaatcatcg ccgttgctaa gccagacgaa gaagtcagac
    36121 tttgtaagaa aacccctgta gcttctgctg tatttccgtc catcaaagtg actgtacttg
    36181 ataaagacat caaaataaac attacggctg tacttagaat tcctgcatga acaggactaa
    36241 atgatggatc atcaatcacg cctgtttgga tatagtgggt tgccaaattc cagccgatat
    36301 tgtaacacac gataatagct aaaatactca tcgtaccatt attcacgatt gaagcaagtt
    36361 cagccaatgg acttagatca agtattcttt ggactgttgc atttgaaaaa atcagagcat
    36421 tcagcaaaac catgacagaa gcagccatta ctaacggcat agtaacgata aaactgtctc
    36481 gaatggccag caagtgtcgc tggctactca tcttagatac gattggtagc attttttcag
    36541 atagtctgtc tacgactttc attatttcat ctcctagatt ctaaaattga accggttcat
    36601 ttttaatata gcactttttt taaagattgc aacacatttt tttaaaacgc tttctaaagg
    36661 tggtggattt tttatgactt ttgaaaaatt attctattta atggtatttt gtgaattttt
    36721 tctcttccat ccgtttgaag ttttactttc caatggtata atttctttat cctatcaaat
    36781 gggggaagta ttttgaagaa attaatcgta ctatttattt tgtctgcggg acttttgaca
    36841 ttgactggct gtgatttgtt tggcggctct gaccaatcct cggacgaaac aaccgattct
    36901 tctgtcttaa aagcggaaaa agcagatatc accaacagtg tatatacgct aaaagtacgt
    36961 aaagatatgg atttatccgc gggacagttg acatttacag ataaaaaaat ggaatggaca
    37021 cgcacgtatc agacacaagg agctgattct tctgaatcat ctgaagaaaa aacagtgctg
    37081 acagatatca agattactac caaagatgac gaatatatta tttcaggaaa agaaaataaa
    37141 aaagaagcaa cagtcacatt taccaaaatc ggtaattacc gtttgaagga taaggatggc
    37201 aacatctaca gtctatagtg ccatctgttg gatcttcttt tgtttctccc cttcttttct
    37261 gctatagtaa agaagcaaga tgactgtaaa ggagacgaaa tggatgatcg atagtcctga
    37321 ttattgtaca tgtaataaca acaaaaatat ccatacagaa tttaatgatc aaggaagttt
    37381 gagtatatgc gacaattgcc acaaactagt caaagattct tttaaatcgt ttgaatctta
    37441 tcctgtcaaa aattatgtaa aaagcaaaag aagataagca aaatcaagca aaactcacta
    37501 tggctgattt ttatgaactt caaactcagc ttcttccttt gatcaagctt caaaaactgg
    37561 aatcagtaaa acaaacagtg gatcatttca gttcacaaat caaagagtaa cgttttattt
    37621 atttcacatc tttgaatata aaataaccaa acgatagaaa gacttattct acatcacgta
    37681 gtaatttttc tttcccgtcg tttggttttt tacttttagc ctaattcttg acctttcgca
    37741 tgctgattat ctgtaaaata ttttccagca ggctttcctt ccaattcagc taatttttct
    37801 tggatctgac gaatcacctt ttttgcatct tctggatttt ctacaaaatc gaatgtatcc
    37861 ccatccactt gtattttagg acatacatca aaatcctcat accattgatt atagcgggtg
    37921 ttcagtgttt cgtaataggt gtataacgtt tcatcgcttt ctagctgttc atagtcacgc
    37981 ccgcgttttt tgatacgttc tagcatcgtg tccaaagaaa ctttgatatg gaccatcaaa
    38041 tcaggacgtt tttttggagc aacatcatct aactcgttta gcatcgtatc taataagcta
    38101 tcgtactgct gtacttcgat atccgttacc cgacctaaat cagcatttag atggaacaac
    38161 agactatctt cgtagatgga gcggtctagc acattgtcat cgttcactaa agcatttttc
    38221 attgctaaaa atcgcttatt caaaaagaag atttgcaata aaaatgcgta ctgctcagga
    38281 tttgaataaa aaagcggcaa tacttcgtta tcttcgacat tttcataaaa tggtttagaa
    38341 tcaatctctt ttgcaagcat atccgtcaaa ctgctcttac ctgcaccaat cgttcctgaa
    38401 atcaaaatca ctgacataca acccttcctc ttctataagt actcgcctac gtaagtttca
    38461 acaggcaatt tttcaaaatc atattcacgt acagcttggt ctgattttaa atacgtgtga
    38521 agactttgtg taatcatcaa gttcgtaggt accgtctctt cttgtacgat gatcatcggt
    38581 tttttcaaca tagttgccac acctaattcg taagccgttc ctggatcgat tgtttgatgc
    38641 tcaaagtcaa ggatagccaa aacaaattct gcatttgtca actcttccat atctttttcg
    38701 tatacttcct gcgcccatcc agcgctgaat agttcataat tgctttcttg gtgatgacgt
    38761 ggagagtaga aacttgtgac tgttttgttt tcctccaacg ctttttcgat acgacttact
    38821 cgatcgattt gctcctctga aaaaaatggt cctgctaaat atatttgact cattattttg
    38881 cctccttcaa taataactga tttttaatag atactgctcg taattcattg accagttcca
    38941 atggattgtt ggaaaagatc acttgatcca accatttttt atccataaaa tcctcttttg
    39001 caaaagtttc taataattgt tgcaagccgc tgtaataacc gccaatattc aatattccga
    39061 ttggcttatc aggtgtcagg ccgatcgcta tccaagaaag aagttgcgaa aattcctcca
    39121 atgtacctag acctccaggg aaaacgagga cagcatctcc tttttcaaat aataatacct
    39181 ttctttcatc aattgtctct gtaggaataa atgtagtaac atctttccca ggcagctcag
    39241 tttcaaaaag gccatttgga tagatacctg tgacttttcc gttattttcc agtactgttt
    39301 tactaatcac tcccataatc ccggagtatg aaccaccgta aaccagttcg atttcttctt
    39361 gtgccatata ttttcctaac gtttgcgcaa taaatttgta actttcttta ttgccaaaac
    39421 gggaaccgca aaaaacagta agtttcataa aaaaacactc cctaactcaa aagtctatct
    39481 tcattctaca cgtgtttttt taaaataaaa gactagattt tgtagttttt ta
//
LOCUS       DACDUU010000020        39217 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.020, whole genome shotgun sequence.
ACCESSION   DACDUU010000020 DACDUU010000000
VERSION     DACDUU010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.020"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            303..2414
                     /locus_tag="IWL30_001052"
     CDS             303..2414
                     /locus_tag="IWL30_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP9705836.1"
                     /translation="MEGIMENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQ
                     IKENNNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAH
                     YQKVGTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQ
                     SINVMNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQ
                     QRVSWYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSK
                     VVGGRAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTAL
                     LWTPQPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKS
                     FKITDTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSF
                     LANAANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPN
                     KKVEQDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQD
                     PVEDRLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREI
                     QLIFTAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLT
                     VIGSILFLTLASLGYVVLKLK"
     gene            complement(2452..4509)
                     /locus_tag="IWL30_001053"
     CDS             complement(2452..4509)
                     /locus_tag="IWL30_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAP9705837.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYMVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVDILKEQIINVTHGESVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFSTPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(4623..4835)
                     /locus_tag="IWL30_001054"
     CDS             complement(4623..4835)
                     /locus_tag="IWL30_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705838.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            5022..5489
                     /locus_tag="IWL30_001055"
     CDS             5022..5489
                     /locus_tag="IWL30_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705839.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            5486..5728
                     /locus_tag="IWL30_001056"
     CDS             5486..5728
                     /locus_tag="IWL30_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705840.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            5746..6048
                     /locus_tag="IWL30_001057"
     CDS             5746..6048
                     /locus_tag="IWL30_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705841.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            6120..8174
                     /locus_tag="IWL30_001058"
     CDS             6120..8174
                     /locus_tag="IWL30_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705842.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKKKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELGQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            8174..10786
                     /locus_tag="IWL30_001059"
     CDS             8174..10786
                     /locus_tag="IWL30_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP9705843.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            10783..13314
                     /locus_tag="IWL30_001060"
     CDS             10783..13314
                     /locus_tag="IWL30_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705844.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PEQAKGMAGNDKSGLSDIQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            13366..13698
                     /locus_tag="IWL30_001061"
     CDS             13366..13698
                     /locus_tag="IWL30_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705845.1"
                     /translation="MDKYSNMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            13699..14292
                     /locus_tag="IWL30_001062"
     CDS             13699..14292
                     /locus_tag="IWL30_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705846.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            14455..16149
                     /locus_tag="IWL30_001063"
     CDS             14455..16149
                     /locus_tag="IWL30_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP9705847.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEIVKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            16837..18714
                     /gene="ltrA"
                     /locus_tag="IWL30_001064"
     CDS             16837..18714
                     /gene="ltrA"
                     /locus_tag="IWL30_001064"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP9705848.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            18845..19222
                     /locus_tag="IWL30_001065"
                     /pseudo
     CDS             18845..19222
                     /locus_tag="IWL30_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            19222..20427
                     /locus_tag="IWL30_001066"
     CDS             19222..20427
                     /locus_tag="IWL30_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP9705849.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            20443..21087
                     /locus_tag="IWL30_001067"
     CDS             20443..21087
                     /locus_tag="IWL30_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705850.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            21098..21904
                     /locus_tag="IWL30_001068"
     CDS             21098..21904
                     /locus_tag="IWL30_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705851.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            21926..22156
                     /locus_tag="IWL30_001069"
     CDS             21926..22156
                     /locus_tag="IWL30_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705852.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            22702..22977
                     /locus_tag="IWL30_001070"
     CDS             22702..22977
                     /locus_tag="IWL30_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705853.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            23019..23441
                     /locus_tag="IWL30_001071"
     CDS             23019..23441
                     /locus_tag="IWL30_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705854.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            23460..25052
                     /locus_tag="IWL30_001072"
     CDS             23460..25052
                     /locus_tag="IWL30_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705855.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            25064..25399
                     /locus_tag="IWL30_001073"
     CDS             25064..25399
                     /locus_tag="IWL30_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705856.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            25546..25740
                     /locus_tag="IWL30_001074"
     CDS             25546..25740
                     /locus_tag="IWL30_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705857.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            25772..28144
                     /locus_tag="IWL30_001075"
     CDS             25772..28144
                     /locus_tag="IWL30_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP9705858.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSSEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            28205..29665
                     /locus_tag="IWL30_001076"
     CDS             28205..29665
                     /locus_tag="IWL30_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP9705859.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFIPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            29676..31880
                     /gene="topB"
                     /locus_tag="IWL30_001077"
     CDS             29676..31880
                     /gene="topB"
                     /locus_tag="IWL30_001077"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9705860.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAESIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ENDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            32119..32712
                     /locus_tag="IWL30_001078"
     CDS             32119..32712
                     /locus_tag="IWL30_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9705861.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            32728..33627
                     /locus_tag="IWL30_001079"
     CDS             32728..33627
                     /locus_tag="IWL30_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP9705862.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            33630..34115
                     /gene="ssb"
                     /locus_tag="IWL30_001080"
     CDS             33630..34115
                     /gene="ssb"
                     /locus_tag="IWL30_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9705863.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            34481..34741
                     /locus_tag="IWL30_001081"
     CDS             34481..34741
                     /locus_tag="IWL30_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705864.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            35182..35388
                     /locus_tag="IWL30_001082"
     CDS             35182..35388
                     /locus_tag="IWL30_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP9705865.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            35388..35639
                     /locus_tag="IWL30_001083"
     CDS             35388..35639
                     /locus_tag="IWL30_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705866.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            35654..36091
                     /locus_tag="IWL30_001084"
     CDS             35654..36091
                     /locus_tag="IWL30_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705867.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFESIEKNGAFYTIESEEKDNE"
     gene            36084..36791
                     /locus_tag="IWL30_001085"
     CDS             36084..36791
                     /locus_tag="IWL30_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705868.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGK"
     gene            37172..37351
                     /locus_tag="IWL30_001086"
     CDS             37172..37351
                     /locus_tag="IWL30_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705869.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            37692..37955
                     /locus_tag="IWL30_001087"
     CDS             37692..37955
                     /locus_tag="IWL30_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAP9705870.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            37949..38299
                     /locus_tag="IWL30_001088"
     CDS             37949..38299
                     /locus_tag="IWL30_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP9705871.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(38323..38937)
                     /locus_tag="IWL30_001089"
     CDS             complement(38323..38937)
                     /locus_tag="IWL30_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9705872.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRL
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
BASE COUNT        14386 a         6252 c         7947 g        10632 t
ORIGIN      
        1 tttttgataa ttaggatgaa atgatgaaaa taataagaat attagttttt aatctatcct
       61 ttttagccgc aacaatattg tgttgcggtt taattttttc tcaattatca atttatccaa
      121 gatatcaacg attgttcatt cattttctaa acattcaatg tgcttccatg ccattagagg
      181 tgattaaaaa gctcttgatc aatccaattt ttatggttag tttggttctt cttacaggat
      241 tggtaacgtg ggtattagga aaaaaagaat gaaaagctta cgatcgttta aaaaaatatt
      301 agatggaggg cattatggaa aataaaattc attgcactac gggaagagta attactttag
      361 tgttgttttt gttccttctt ctttttgcga ggggaactgc agtccaggcc ttagaaaaag
      421 ttgatccgag tcaaatcaaa gaaaataata atctggtgct ttatcatgat agtgattcat
      481 tgagaaatat cgaagtaact gggatcaatg gatttaagat actagatgag tattcaacta
      541 aaaaacaaaa agtacttgct tggccaaaag tcaaagattt gaaaagtacg tcaaaaatcg
      601 ttgcccatta tcaaaaagta ggtacttatc taggaaaaga aattgattgt gacgtgactt
      661 tttctaattt tgtagcgcac gctggggtta accaagacgg attagctaaa acaattaatg
      721 gcaaaaagta ttataattat ttgctctttg ataaaaatat ttatcgagga tttttcatgc
      781 agtcaatcaa tgtcatgaat gcaaaactag aatttaagta tgcaggaaca aaaacaaaag
      841 tagctttgag tgaaccacag ccagaaattc aatcaaaaat gagatcgatg agagcagctg
      901 gtattgattt aaacgataca tttctatcca tttacagttt aaatggacag tatcagcaac
      961 gtgtgtcttg gtataatgac aataatgaat ctgtcggtga acgtaatatt gatatgagag
     1021 aatttgttgg gtatgaaaaa atgggtagct taccttattt tgtcacaaca gatacagcat
     1081 gtgcagaata caaagctcct gcgttatcaa caaacaattt aacttcaaaa gtagtgggag
     1141 gacgtgcaga aaaggcttat agctcgaatg atcatttcac cgatgttgta ggagctgata
     1201 cttatcacag aagtggtgta acgtatacgc ttcaaggcgc ttccccaaca ttcatgattg
     1261 gcgcaaatac gaatagtatg atgtttagct ttgatactgc attgctatgg acaccacaac
     1321 catcgaagcc tacaaaagaa gtgtttaaca aagctaatac tgaagaggca gcacacaata
     1381 ttgacaaaaa agtgattcca caaggatcag atgtttacta tcatattcac caaaagtttg
     1441 atgcattaac agtcaacaca atgaacaaat acaaatcatt taaaatcact gatacctttg
     1501 acagcaaaaa ttttgatatg gtatcggatg ggaaaaacta tgatggcgca ttgtggtatg
     1561 ttgaagcgaa tggtacttgg aaaaaagctg atacaggagg aaagttaact gtatcaggta
     1621 atacagtcac ttatactgct tctgacagct ttttagctaa cgctgcaaat tacggtaggg
     1681 attatatttt gcagctccat atgagagcaa aaaatgatgc aaaaggaatt gcaccaaatg
     1741 tggctactgt aacgtttaac aatgatccga acaaaggaac acaaaaaaca aatacagtag
     1801 aaaactattt ctctcttaaa ccaaataaaa aagtggaaca agatgggaaa gatgttgatg
     1861 gtcgaaatga tggtaaaaaa ggaacgccta cagctccttt aaatgcagga tctgaagtac
     1921 aatatctagt cacacaaaaa tggcatacaa aaggtgtaga cattgcctca gatcattaca
     1981 agcaatttag tattcaagat ccagtggaag atcgtttgac gtataaagaa ggttctgcgc
     2041 gagtgataga tatgagtaca ggaaaagata ttacttcaga aggtacacta acctatgata
     2101 gcaattcaag aacgctgaag tgggaagctt ccgctgattt cttaagtaaa aatcttttag
     2161 atggacgaga aattcagctg atatttacag ctaaaactcc tttgaatgaa gaattggata
     2221 tcgacaacca agcaaaagtt tcagtagaaa gtattgagaa agcaactaat gtagtaacga
     2281 ttggggtaaa acctaactta cctcagatca ttgtgccaaa aactggatcg aatcatttag
     2341 tgctaactgt tattggaagt atattatttc ttactttagc atcattgggc tatgtagttt
     2401 taaaactcaa ataaatataa aaacaaggct gagaatcagc cttgttttct tttatttgaa
     2461 gattggattc gcactaataa atacattatc tacgccaatt ctaccagcag agtcaactac
     2521 aaaattatga cctaatccga gaatactttg gttattataa agtaaatttg cattatgggc
     2581 agggctattt ttccatccta ccatggcatc gatagcacca tcgtctgata aaaggtcatc
     2641 aatatacagc ccagtttgac ttatgttttc tcccttagca ccgtagcagc ctaagctttc
     2701 caataaatct tggaaagtac caccaccagg gcgatagtga ctgaagctga cttgtaattc
     2761 tttagctcga atatctgcag cctgttgaac aatatcattg aattgcattg gggcaacacc
     2821 attttgttgg cgataatcat ttaaccaagc aagttctttt tgacgcataa agttacgtaa
     2881 ttcgtctaat gaaatctttt tatatttgaa tgtgattgtt ttattttgat cgacgttatt
     2941 aatggatcgt tggccgtttt tatccacggt aacggtagga tcgtcatttc tgcacaagaa
     3001 ataattttga tcagtcatat ttttccaagg agtagagaag ctatcccctt cgtatactgt
     3061 ttttgattca ctagagagaa cagcgttggt atcagcattt acatgattaa cagtcacatt
     3121 aaatttacga gtgtagttga aaatataatt ttgactttca gtcacattgt ttaaggttac
     3181 tgaatttgtt ggttctaccc gatcgtctaa aacatatcct tgaatagaag gagcagttaa
     3241 agtgacactt tcaccgtgag tgacgttgat tatctgttct ttcaaaatat caactcgccc
     3301 ctcatttttg tagttgttat tgcacaacaa attgatttga tgcacttgat tataattgaa
     3361 tgtgattgtt gtattacctg tgattgtttt gctttgagta tcattaccaa tcactttgta
     3421 gctaaaatta tctttagcac cttcgctata tttaaatgct tttgccttag cagtgtaagt
     3481 agaaccagaa gtcacttttg cttgatctgt agcaatcact tgacctgttt gattgtcaat
     3541 atggttaatg gtaaccatat attcttttgg cgtttcaggt ttgtgttgca accctttaaa
     3601 tgcagcatca agactatttt tagcattgtt tacttgtgtt tgagaaacct tagaatcgtc
     3661 tagtactttt ttagcattgg ataaagcagt ctgaaaatta ttccaagatt cttctgtaaa
     3721 atctccttta gcagttgatt ttactcggtt gtataaagct tgtaattcag ctttattcac
     3781 agtaggaact ggttttttct ttaatccttt gtaagcagaa tctaatttat ttttggcact
     3841 atccacttgc ttttgagtcg cttttgagtc atttaacact ttcttagcat tgtttaaagc
     3901 ggtttggaat gcgttccagg tattgtcggt atagtcacct tttttcgttc ccttaacgct
     3961 cgtataaaga gcttctagag ccgatttatt cactttgggt ttagaggtgt cagcgccata
     4021 ccaaccgata ccttcgtatt tccagccata gtctttggtt aaccattgta cttctttcca
     4081 acttgcagtg tagtgatgtg agccagattt tacaccagga aggaaaaggc ggtagatcgg
     4141 tttattattt ttttcggcag aacgccaggc aggcccttca taagtccagc cgtaattttt
     4201 agtcaaccat ttcacttcat cccaactagc tgtgtaatgg tgatcacgaa gacctttatt
     4261 gtacaaacga tagacaagat ggccaccgct agtcggtgct tcccaggcgg ggccttcata
     4321 atttccccat tttatcttta ctagatggtc acgttctttt ttgctagagg tgtaaaaatg
     4381 ttcattatta tttggattgt aaaggcgata catgacgtta gttgaagcag cgtcaacaga
     4441 tccagcaaga ctaagtgagg cagcaaatgt tccaatagct aaaacagttt ttggattaaa
     4501 taatttcatt ttttcaactc ctaaatttaa ttaaaaatgt ttacatgatt attatatatc
     4561 ggctaaccga tatagtcaat aaaaaataag aaaagcctta tataattttg gcttttctta
     4621 ttttatttgt tagttgaagt attaatatca acgatttctt cagccaattt atttaatttc
     4681 atagcattta tgaatctcat gttgtctata ctagagcgtt tgttggcaag gtcacttact
     4741 gtagttaagg gtacgccaga ttctatggat atagaacgaa ctgatttacc actatcaaac
     4801 agcaaccatt cgattttctt tttatcagcg tacatttcaa tgctccttct tgttttttct
     4861 ttattatagc tcttttttgt aatgaatcac aatatcaaga gaaaaatttt ctatagaaag
     4921 gtggattttt atgagtattc gttctccgtg tatccaaatg aggtttgttg ctactttcgt
     4981 tatttgatat actaaaaata aacaacgaaa gaaggaataa aatgaagaaa aagcaactat
     5041 ggtttgtctc tatgtttgca atatttttta ttattttaag tggatgttca agtaataaga
     5101 aatcggaagc taaagaagat aatagtttat tgcaaccagg aactgtttgg aaagaagaag
     5161 taggtggtct tattcataat ttaaaaatta tagatgatac tacctgggaa tattctgaaa
     5221 gtgtatggca tccagatcct gtacaaataa cagttaagag gcaaaaagac tatgaaggtt
     5281 tagaaagata caaaatagta gattctgcag gcattaggga atttataaac aaacctgatt
     5341 cattatttat tgttgttccc tatgagaagg atggcgtaaa aaaaataatt tttcttggat
     5401 ctagcaagga tgagaagcga acgaaagaga aattaattca tgatggatca gagttaaata
     5461 aatacaaatt acaaaagact agtgagtgat tgctagtctt tttatttatc ctgaaggagg
     5521 aatcacagtg gcaaaagtta aaacaccatt cgaaaaagcc gcgtctaaaa tcttagaacg
     5581 ccgtggtgtt tctgaaatac agtggaaaaa agaagttctt gaaaaagcac aatttgacct
     5641 attacgagga aaagataagg acttagaaga atatatctat caacgtgaac gtgaaaaact
     5701 tgtattcgat gaaattatta aacattaaaa ggagcgaata atcttatgtt tgaatcaatt
     5761 acaatgaatt ttttaactca accgaaagta gtagctgcag ttgatatttt tagagcatta
     5821 caaactgccg caactagttt gaaaaataat ctgatgattt ttgctgctgt tgcggcagtg
     5881 gcttccattg ctcttggagg tattttcttc ttctttgggc gaagaggagc tgaaaacggg
     5941 aagacacacg taggttatgt attgttgggg gtaggagtta ttttagctgc cgcttctatt
     6001 gtagtttcaa tgttctcaat gtttggacaa accccacaag gactataaaa aaaacaagta
     6061 ggaggagtaa aaagtacgtg gctacttttt actccttttt ttatgaaagg agaatattca
     6121 tgttttttac gatttcagga aatgcaaaat tggataaaga gttaataaga aaagcaacga
     6181 agaaagtggg aaagcgtact tcctttttac cgaaaacaat taaatattcg gtcattgaaa
     6241 cggaagaaca gctacatgtg ttaggacaag tgttagaaca agagaatcaa tcttctgtta
     6301 aattgactct cacgttacat gataaagaag gaaaaataga aaaggctggc acagaatacg
     6361 tttttggccc actggattat caaatttcag aaggttttta tcctacattg atagatttaa
     6421 tccgccttga tgtgtttaaa gatacggatg aaactatgag tgtggaagag tcttatcgaa
     6481 acgaaaaact tttacaagtt ttagaagaag agttagagag cgaccaaaga aaagtgaatc
     6541 aagtgagtga agtcgaagta aatgaagaag taccatggga acgaccaata tttccaaaga
     6601 aggagtcaaa agaaaaagag acagtctatg ttgacgaaca agctgatatt ccgactcaga
     6661 aatcgatatt tgaggaagag aacgatgtga tgggtcaatt ggaggtagat ccctttgaag
     6721 aaacaatgga ggatgaagag acggacgaag tagaagttga tccagttgaa gctaatgaag
     6781 agtcaacatg gactgaaggg gattttcagg aatatattga accacttatc agtgaagaag
     6841 tagaaaaaaa cgaaacagag atacaaaagg caactgtatt tgattatatt ttggatcaat
     6901 atccgccaga gcatgaatgg atgaaaaaaa aaaacaaatt atttttaaat gagctttatt
     6961 cgaaatatca attaccccaa acctatcaag catttttaga gagtaaagaa gaattaatct
     7021 cacagggtaa aggcacactt ttagaacagt tagagtctgt ccaatcaaca aattggcatg
     7081 agcttggaca agaagattta ttagccacct ttgaagaacg tcaacaagaa acagatcaag
     7141 aaattaatga gtatgcgacg gaacagtcgt ctaattggga gaaagtaaaa gaggaactaa
     7201 ataaagaaga acagcgagtg attgaagaag aagtacaaaa agtcaaagag cggtacgaaa
     7261 agaaacgcga caatgcttat tccttgtgtg tagaaaagat caacaatttt actaagacga
     7321 atcaagaagc gttatcgaaa gaaaaagaaa acttgttaaa agaacgtatt aaagaacgaa
     7381 aagaagagtt ctaccgcggt ttacgtacag ataagctaaa gattagcaaa gaattaaatg
     7441 aacaactgaa taagctatat caaacaacga ctgaagttct tatggaaaaa cacgaacaga
     7501 tccagcaaga attacaagta caaattccaa aatggaaaaa agagtatgaa attcaacaac
     7561 aagaattgaa agaacaagaa gaaaaacaac ggcaaaaaga aaaagagcgt gaacaattag
     7621 aaataagaaa acgggaacag gatcttgcgg aacaaaaaat ggcgatagaa aaacaacggt
     7681 tagagcaaga aaaggaaaaa gaagcagctg ctcgtaagga acgtcaagaa caaatggata
     7741 cattgcgtca agcacagttg aattatctag cacaacctca accagtagct gctgttcaaa
     7801 cgccagtgaa caaccaagca aatttaactc cttcacccca gcgttccttt aacggatgga
     7861 tggtgggatg tattgcaagt ttaagcttgc ttctaggtgg aggaacagta tttgctttta
     7921 atcatatcaa tcaatcttca caggctgatt ctgtagccac aacggcgtca atcaaggaag
     7981 aagcccagga acaagcaaga aaagagtatg aacaacaatt agaagaatta ttgaatcgta
     8041 ccaatcagtc aacaacagag tcaagcatgg aaaatagcaa agagagtgat tctagtaagg
     8101 ctaaagaagg atcaagcgat tcaactgtac aatcaaacac ttccgaaagt tcagaatcaa
     8161 atgaggtgaa ataatgcgtg ttagagaaac aacgaagcga aaagacaaac gattcaattt
     8221 agaaaagaat aggtatcttt ctcatttcta tcgctatact ccctcaggag aagagctacc
     8281 agaaaaacat gacaaacgca tttcgaaaaa gattttttat agtggacttg tatacctccc
     8341 tttagagtat ttagtaacaa gagtaccaga agttttcaac cgattggaaa ttgttaaggg
     8401 aagcttacaa ggaataaatc tgtttcaatt acaggaaatg ccacaggtct ttcattttga
     8461 ttcagctggt ttttggaatc catttactcc tacaactagt ttgggtacac gtttagcaat
     8521 ttcgacagtt tgtgtggttc cagctgtact tatgggattg aaaatggaca tgtggtttcg
     8581 tcctctagca gatggacaaa aaggggataa tcgattaatg accttaaaag aaatccaaca
     8641 gatttatccg caaatacctg aaacaaaaac tacttttcca ggttatggag gtattccaat
     8701 cacacattac aaaaaatatt ggtacattca aacagatact gttaatacgt gtattgttgg
     8761 tacctctcga tcagggaaag gtcagattga agtgttagcc accattgaca atttatctcg
     8821 tgcagaaaaa caaagttcaa tggtcgttaa tgatccaaaa acagaattat atgttggtag
     8881 taaagatgaa ctagaagcta gagggtatga tgtatatgcc tttaatatca ccgatccact
     8941 gcaatcaatg agtgataatc cattggcact catcgtgaaa tactggaaac gaggagacat
     9001 agatactgca acacagttaa ccaatacttt cacaaatacg atttattatg atgccaacgc
     9061 ttcagataac aaatacttta acgaaaacgc gcagaaagct gtcaatgcgc ttatttttgc
     9121 gttgttagaa caagctgatc aaacaggaga ttactccaaa ttgacaccga ataatctcgt
     9181 tgagcttctt tctgagattg gcggttttaa ttattatcca gatccagaca atgaattcaa
     9241 acagttgaat gccttagatg aatttatgaa tcagttgcca cctggaaaca ttgcaaaaaa
     9301 acaatatggg gcaacgaaaa ttggatctga taaagcgaag ggaaatattt tatcaacggc
     9361 aatcacggga ttgaatccat ttactctaac gaaaattgca aagatgactt ctcaatcaac
     9421 gattgattat aaatcggttg ggtttccaaa atacttagat ttgaaattac atgaaagtct
     9481 tttaaatcaa cgagtcgtag tagaatttta tcataaggga aagaaatttc atgaagaacg
     9541 tgtcaaagta ggctataaag gatttagtga aatcaatttt gattgtcatt tagttgcagg
     9601 tgatcaagtt tgtttaaaat acgatcatca aggaaaagaa tatgtggctt ggtatgaatt
     9661 ttctcaaaga gtattgaaag acgaagaggg taatatcgtt tatcggaaaa aacgaggaga
     9721 aacccatttg ccagaattag aaaaagaagc cgttctaaca ttagatgagg aaaaaagtaa
     9781 cttattagta gagtctattc ggatgcacta taccgataaa ccaatggcta tttttatgct
     9841 tacacctgat tatgatccat ctaaccatat cattgttagt atttttctta gtcagttata
     9901 taaagaatta tctacgcaat gtgttaagac taaaggagat aaatgtcacc gcaggattca
     9961 ttttatttta gatgaatttg ggaatatgcc accaatggat aatatggaag gaatcatgac
    10021 ggtcacagct ggccgaaaca tgttgtggga tctgtttatt caatcttatc agcaacttta
    10081 tgcgaagtat ggagaacaaa acgcagcgat tatcaaagaa aattgtcaga cacatattta
    10141 tattatgtcg acagatgata ataccatcga agaattttct aaaaaagtgg ggaataagac
    10201 ggttgaacaa gagaacagta ccatcaattc attaggtatg aatactcata tcaatcgcaa
    10261 tgttgattca gatcgtatct taactcctga aagaatttct acttttttag aaggagaaac
    10321 gattgtactc gctcctttaa aacgacaaga tttgaaaggt cataaaatac ggccgtatcc
    10381 tatttttaat catggggaaa cgaatcttcc ttatgcttat caatatattg aagatttcaa
    10441 tccatcgtgg gatatcaatg atatagacat acattgtaca catgcggact tggatcttaa
    10501 ggctttacaa attgattatg attatttcct accagatgaa tatgctagaa atctctatca
    10561 tggactgcat gatcaattag aagaaaaaaa cgaagaaaat atgttttata aaatcgttca
    10621 atctcttgag aatgagacgg ctaaaaaatt gttaattcaa ttttacgaag aacaagcagc
    10681 agatcgactt caagaatatg ttgaaaagtt gatcaaaaat aaagaaattt ctgaaatgat
    10741 tgggaaacgg ttgttattgg aatctgaaaa ggtggtgaaa gaatgatcat tggaggaatt
    10801 ttttctggca tagccgactg gtggtcaggc ttattcggag aaggggatgc aaaccccaaa
    10861 accgtaacga aagtcttaca agattatggg aattatctgc agtatcaaga tgtatttagt
    10921 tggatttgga acacttttct gtggggattg attaaaggat tatataatct caatacttta
    10981 ctagaaaaaa ccatttatca aagctttgat ttaaaagact tattgaacgc cgctggggta
    11041 aatgcactat ttcaaagttt gattagtaag gtattggctc ttttttgtgt cgtcacatta
    11101 atttatttag gcttgaaatt ttttatgagt aaacacccac caaaagtcaa aaatatcttg
    11161 gttaatttat ttatggccat gatcttgatt tttggtggta gttctttaat cgatgaaggg
    11221 ttaaatatca gtaaaagctt ttacggagac gtcactgcag caaataaagc agataaaacg
    11281 ggatcacctg cttttcaaat tattcaaaat aatgtgatcg atgtcagtac gttactagca
    11341 actgacccga ataaagtaga atccctacca acagataaaa gaaatgcttt aacagcgaaa
    11401 aattttaaaa cagcgaatat caatgggatt attactcctg aacaagctaa aggtatggca
    11461 ggtaatgata aaagtgggtt atcagatata caaaaggatc ggttaaaggc attacaatac
    11521 cgattggaac tagatgataa tgggaaagaa attcctgttc aaattagtga cgaaggctta
    11581 gcaaaatatg tctatacttc agggtatcgt cgatacagta gtaaaccagg tattatttta
    11641 gctggtgaat cttctttggc agtagcctat ttatttatcc tatttacgat cgttacttgc
    11701 attattgagc tggtatttaa aaaattctat ttagtgattg ccgcgtcaac ggactttgag
    11761 acaggtcaac ggatgaaaag tgcgatagaa gatatttctc aatccttctt gttattggcg
    11821 tttacgattt tagaattacg aatctatacc ttaatgcttt caggtatcgg tgatttgcat
    11881 gcggcaggaa agatcaatgg attcttgtat gtcgttgctt tgattgtact aaccattgcg
    11941 ctattcaaag gctctcaatc agttacgaaa attttcggcg ttgatacaag cctgaaaaac
    12001 ggcagtaatt ctcttcttag catgtttgcc ttaggaaata ttgcgaaaaa tgtgggtagc
    12061 ggagctaaaa accttgcaag tgctggtaag agtggtttaa gccatttaaa tgatattcgt
    12121 aaaaatggat ttggccatcg aaaagatgtt acccctgata acggggaaga acaatcagac
    12181 gataaagcta caactgtacc taatagtaag aaaacagttt ccgaaatgtt cgggaaagcg
    12241 aaaaatggtg tttctaaagc cgcagaaggt cttggttacg tcaatgaacg tgggttaaaa
    12301 ggaacagctg aagatcttac agaaaaagcc aaagacacct tagaagataa attagacggc
    12361 acgagagcta aagaattggc tgaagctgtg aagaatccat ccgaagcact agaaaaacaa
    12421 aaagaaaaag cttcagaagt caaagaagga ctaaaagatg cgtatggtga aggacaagtc
    12481 aatgcagcag tgaaagctaa tacaacaacc gaaaaagaaa atcaagaagg tatagtgatt
    12541 ccttcagtca aagatcacca agaagcgttg cactctaatg aaaattcgat tgaagagaaa
    12601 aattccatag aagaaagaaa ttcagaagaa ggaaaaaatg gacagttacc aaaaactgat
    12661 ccagaacaat atcgctataa acctaacagt gaccttgaaa atgagaagcg aatttcagat
    12721 ttatcaaaag taaatcaaga agacaaagca gaaaatgagg gacagttacc aaaaactgat
    12781 ccagaacaat atcggtatag atctgttaac tccgaaaatg aaaaacgcat ttcagattta
    12841 ccaaaacgaa atcaagaagt acaaacggat gaggattcca tcgttctgcc aaatgataat
    12901 ggaatcgaag ttggaactga aaaatcaacg aaaattggtt tacctaaggc acaaaattta
    12961 ccaatgaaag atagtcgttt gaatcaattc aaggataatg atcaaagttt tgttcatcaa
    13021 tcacctgcag gtactgttca aatgaaacca agtcgtttgc ctgaaaatgg aaatctgaca
    13081 actgtttctt acaatttatc tgaaagtggt gagacaggga atgcgtccgt tcaaaatgtt
    13141 gtgacaagag aagctgcacc aaaacctatt caaaacgttc atacaggtta caagccagca
    13201 aatgtggcat ttactgcacc aacaaaaata aatgtgacaa gagaaacaaa aaacaggaac
    13261 aatgcattga aagaacaact attgagggat atcaaacagg atatcaaacc ataaaaggcc
    13321 tacaagttag gccttttttc tatgtatgaa aaggagaaat catttatgga taaatatagt
    13381 aacatgaaaa accctccaag atttagtgga gaatggaagt ttttcgcttt tttcttttta
    13441 gatctattaa cgcttggtgg ctgctttttt tgggcgaaaa tggtcaataa tcttgtccac
    13501 ttaccactct tttttgcact cttgaattat ggcttatgga ttgcgatagc gttagtgatt
    13561 gtttggcgac cctataataa cccaggtcaa cgacaaattt ttgcgattat cgagtctatt
    13621 ttttttgcgg acaagaacca ttatcatgcg ttagatccta accgaaatta tcatggtacg
    13681 tataaggagg atgaataaat gcgagtcaaa ggacaaaatt cagttgaaag gaaatccatt
    13741 ttgcagtttg gaaaaccaga agagcgctct atcttggact tatttccata taaatcctgt
    13801 acagaagagg gagtcttaat tactagagaa gatcattttc aacgttatta tcgtgtagtt
    13861 tctacagacg tggaaggatt aaatgaacaa gagaaactag aacgaatgaa tcagttgacc
    13921 accgtcatgc gtacgtatgt gccaacaatt aagttagtta gtttgaccac agagacggat
    13981 ctaacggaac aaatcgtgca aaagcggcaa ttattagata aaaatcgtat ggaacagtcc
    14041 ttaggaagaa atctacaacg attaaaaaaa tatgaaaaga aattagtcga agaaatccag
    14101 gaattgaaac gagcagaaca agaacgtcct gacttaagct tctttttcgt cattgaagga
    14161 aagacaatca aggatttaca aaccagaaac cgtcagttaa tgcgatctag tggcatttta
    14221 gggttaaaac cactctacaa aaaagaattg atcaagattc tgtatcgaat gaacaatatg
    14281 aatgatgagt aggaatggag aagagagaat gaaatatccg ttgatatggt tagaaaaaga
    14341 tgttccgaag gaagagacca ctagaaaatt tcctcacact ttaagcctgg gtaagttggg
    14401 aaaagggcaa tcttttaacc aagtaaagaa tcaaacaaga agaggtgact gtacatgtta
    14461 gaagcaatta aaacagcttt ttcaccacaa aatgatcaaa aagtacttga tccattaaag
    14521 caactaggaa atcgggaaat agaaattgta aaatccaaag ggtatgattt agatttttta
    14581 aaacaagtga tgccacaagg tgggttaaat tttgatgaag aatatgtgac gtatggtaat
    14641 ggttattcac gatgtgtgac agtttatcac tacacaaaaa atcctacgct tttttggttg
    14701 gccaatctca tgaacaatcc tcatacagtg gccacactcg atgttagcac agatgaaaag
    14761 caaaaagtga ttgatcaaat caatcgtgcc ttagatgaat taaatgatcg atcagataat
    14821 gaacgaaaag cgactgaccg aaataaaagc gcggaagaat acctggaact agcggaatat
    14881 gctagaaaat tgacttctaa tggggaaatt tctaagcaag tcaaagtgag agtacatgtt
    14941 tacggaaaca cgattcaaga agtagatgaa cgagcgcagg aactgatcga aaatctcaaa
    15001 gggatggatt atcgtgcggt gacttatctg cataaaacca aacgagattg gcaatcgatg
    15061 tttaccatgt tcgatgaaca agctgaatgg ttcggaaata tcaaagggca atcactacca
    15121 tcgttaaaca ttggcggagg tttcccgttc caccaccaaa cgctaaaaga tccaggaggg
    15181 acacaatatg gggaaaccat gacaggcggc ccatttgtct ggaatgcgac gtataaaaat
    15241 agtgtacggt tgtctactaa taccttgctt ctaggaatga tgggagctgg taaatcgacc
    15301 gttcttaaaa tgatcgcgga agcccattta gctgcaggag actttatttg gggctttgaa
    15361 aagggaaaag actttattcc tttcttaaag gaatataacg gtattatggt gcgtttagac
    15421 ggatctgacg gaatgatcaa tcctttagag atatttgcca cacggacgta tgatgaagca
    15481 tccagtttat atgacgatgg atcagtcaaa gatttgaaga tcaatgaagc agcgtcctat
    15541 caaacacact tagacaaagt ggtctatcag gtacagttag tgagtcctca gctaaaagga
    15601 acgatgaaag cagaatttaa gacctactta aatcgttttt atgaagagta tggaacagta
    15661 ccacgcggct ttacttcctc aaattcgcgt tcaaatactg atacgcaagt cacagggaaa
    15721 gatccagaag cttatccgac gttcaaagaa tttttagact ttttaggaca actggaatta
    15781 ccaggtgctt cccaagagaa aaagaatcga aaagaagaaa tggaatccat cgtagaatcc
    15841 ttatgtgaga cgtatggcat gatttttgat ggtcatagta cgattcgaca ccttgaccaa
    15901 caacagttgg tcttttttga cattgactct attacaggct tagatcgagg ggtacaacaa
    15961 tgtcaaatgt acatggctat gacgttgatt tggaatcagg cattgattca aggaagaaga
    16021 caacgacgtt taattaaaga aaaacaaatg gaagaacaag acttcaaacg cttcttggcg
    16081 atgattgatg agtgccataa tggtgcgacc tgttctgcag tgaaaagctg tgggttgcaa
    16141 gtagtctaaa aactattgta ataactctta taacgagaag tcaaatgagt gggtaacgcc
    16201 acaaagggct tccactgata ctccgacatg caacaagtag tttatgcaga ccaacgttgt
    16261 gtgaagctcg gtgaagtcgg ctgaaggata ccgtaaggta aaagaaatag tgaataccac
    16321 gaaagttctc tagaggtaaa gggtgtatcc gataagtcgg gtgtccaaaa aaacttaata
    16381 tggtgagtat gtacaaacgt actgacgaac tagcgaatgt actgtccgaa aggcatagct
    16441 cctagaaatg ggagagacag tgagcaaaaa atcactcatt gtgctaactt gtggttgagt
    16501 aaaagtcgtt gttatgaaag accatgctgc gttacaggcg taggagaagt tagggggcaa
    16561 aagctagcat ctaagttaag gcatgtggat agatataagg gaacagggga agccatcaac
    16621 atggagagtg catactcgat gaagtggtag taagaaatat gtgaatgaca aataacttac
    16681 tttagtgatg gtgaaagtag agtcacgact gatgaaggtt ctgtaatgga actggaggaa
    16741 caggctcgat tcgtctgtaa aagtcatgaa acccgttcaa ccatgggatt ctcgtatgac
    16801 taaaaaggtc acgagccaat tggagcgtga taaaccatga caaaagaaac aagtccaaaa
    16861 gataggaagt tgcggcatgc agaatattat ggcatgacgg aaatcttcga taagatatac
    16921 tccaaaagtt tgaagggaga taatttcaag aatctaatgc agattatcat ttctgataac
    16981 aacattgtcc tagcatatcg taatatcaag agaaatcacg gtagtgtgac aagaggagaa
    17041 gatggagtaa cgattaaaga tattgaaaat ctcaaccgta atgaattcgt agaaattgtt
    17101 aggaagcgat tcgcacatta taaccctcgg aaagtaaggc gtgtagaaat tcctaaatat
    17161 aatggtaaat taagaccgtt gggtattcca tcaatatggg atagaatcgc gcagcaatgc
    17221 atcttacaag tattagaacc aatatgtgaa gcaaaattca ataatcacag ctatggtttc
    17281 cgcccaaata gatcagcaga acacgcaata gcagattgta tggcccgaat gaatcaaagt
    17341 cacatggaat atgtggtaga tgtagatatc aaaggtttct tcgacgaagt caatcatact
    17401 aaactcatga gacaaatctg gtctatgggt atacgagaca aacagcttct cttaatcatt
    17461 aggaaaatgt taagagcacc cgtagtactt ccaaatggga agttgatata cccaacaaaa
    17521 ggaacaccac aaggcggaat acttagccca ttacttgcga acatcaatct caacgaattt
    17581 gactggtgga tagcaaatca gtgggaggaa aaagatgcca aagagctaaa acatcatttt
    17641 aggaaaaatg gagcttggga taaaggtaat atctatcaca agctcagaaa gagtacaact
    17701 ctaaaagaaa tatacctagt gagatacgca gatgatttta aaattttctg tagaaattat
    17761 aatgaagcga aaaaagtttt ctatgcaact aaaatgtggt tacaggagcg tctaaagtta
    17821 ccaatatctg aagaaaagtc aaaaatcaca aatctttcaa aagaagaaag tgaattttta
    17881 ggctttacga tgaaattagt gaccaaaggt aacaaaaagg ttgttaatac gcatataggt
    17941 acaaaagcac aaagcatgat taaagtgaaa ctaaaaaaac aaatcaaacg aattcaaaaa
    18001 agtcctaata gcaaagatac ctttatctat attggagtct ataacagtat ggttattggg
    18061 attcataatt acttcaaaat tgcgactcat tgtagtgtag caatgcgaaa aattgcaaga
    18121 gaactcagac caagttttga aatacgacta cgagaacaag gaatctcaaa agaaggacaa
    18181 tacggaaagt acgaaggata caagccttac ctacggtcag gacaaatacg atatataaga
    18241 gggtttccaa tattaccagt agggtatatt caacacagac atccgatgtg taaaaaaaga
    18301 aatattaata aatacaccga agaaggtagg gaagccattc ataagaagca acaaaccgta
    18361 gaagaatgga agatatcgtg gttgtgtagc aatccagtgc ttagtaagag agcaacgatt
    18421 gaatttgcgg acaatcgaat ttcaaaattc atcgctcaaa agggaagatg tgcagtaaca
    18481 ggtgaggagc ttatcttatc tgaaatgcat tgtcatcata ttatccctta tcatgaaagc
    18541 aaaagtgata gctatgaaaa cctagtgatt gttactgagg aggttcatcg ggtaatacac
    18601 gctacacaaa gtgaaacgat tgaggaactt ctaaaatatc taaaattaaa tccaaaacaa
    18661 aaagaaaaac tcaatgagtt gagattaaaa gttggtaacg aagaaatatc ttaataaata
    18721 tttgtaggac gggaaaacag aatgtctttt atagacgagg gaacgccgta tgcggtgaaa
    18781 gtcgcacgta cggtgtggac tgggggaaaa gacggagata atatcaaact cttacctatc
    18841 agtatcgatt aatccagatt ttattgaaac ggtaaattat gtcacaaact ttcaacgaga
    18901 aatgcgtaaa gtagaagcaa tgactgtttt agctacacaa tccccacaag aaatggtgcc
    18961 agaaaatatg tcttctgata acttctcgaa actgaagaaa gtctttgaat tttcttctac
    19021 gaagatcttt atgcgtatgg atgaatctat tttacgtcat atagaaggat tagtgggtaa
    19081 atcaatgacg aactcagaat tagaatccat tcctcaacaa aatcaaggag acgctgtgat
    19141 caattttggc tcaaaagaaa ccattcgtgt tcattttagt ccgaacaaac gtcaattaaa
    19201 attgtttgat ggaggaaaat aatggaaaac caaaatgaaa gtttaatcaa gcagtatgtt
    19261 aagcgtcgtg caaagcggcg cttatttttg tggttgtttg gtacttctgc aggtttgatt
    19321 actattttga tcactgtgtt cgtgacccta tttctaatac ttgcggcggg ctcgattgat
    19381 aattcggatt cagattcaag cagtggtggc gaagctttta caggagaata ttcggaaggc
    19441 ttaccgattt ataaagaaat caaaggaaga ggcccttttt ctgatgagat tgcgcaatat
    19501 gccgtaggtg cagcagtgaa atataaatta ttaccaagtg tgatcctttc gcaatacggt
    19561 tacgaatcag cgtttggtac atcggcgtcc gcaagaaatg atctcaacta ttttgggatt
    19621 acttggtttg atggttgtct gtttcctaaa ggaactgcac gaggaattgg tggcattgaa
    19681 ggtggctggt acatgaagtt tcccaactca aaagctgcat tttcctatta cggctttatg
    19741 gtagccacac aatccaattt taatgcttgt gtaggaaata aaagccctgg cgcaagctta
    19801 ttgatcttag gacgaggcgg ttatgccgct gcaggaatta cggaagatag tccgtattat
    19861 acaggttgta tgtcaattat cacttctaat aaattaaccg aatatgatga attcgccatc
    19921 aaacattggg gtgaaggtgg caataacaac ggcacgatta ctggtgaatg gacgaatcca
    19981 ttccctggca gcagtttaga taaaagttcc ttcagcggtg gtcaattatt tggaacgaat
    20041 ccaggcggtg aattccgtcc gaacggcttc catgatggat tagattttgg ttcagttgat
    20101 catccaggaa gtgagattca tgcggttcat ggcggaaaag tcgtgtacgt tggcaatcct
    20161 ggtatttcag gtttgggcgc gtgtgtgatc gtgattaatt acgatggctt gaatatggtg
    20221 tatcaagaat ttgcaaatag tacaggaaat tctcgagtga aagtaggcga tcaagtgaaa
    20281 gttggtcaag taattgggat acgagataca gcacacttgc atttaggatt tactcgaatg
    20341 gattggcgtc aagcacaagg acatgcattc attgatgatg gtacatggat cgatccgtta
    20401 ccatttttga atagcagtaa aaaatagatc aggagaaaaa atatggaaaa taaaacgtta
    20461 aaaatcgtct taacagctgc aattagcgtt agtttaatcg gcaatattgc gttgttttgg
    20521 cagctgaaag aggaacaagc agcaaatacc tataaaattg aagaagtggc taaaaacgcg
    20581 tctgagaagc tcaaaaaaaa ttcctctgag gcacaggcga agatcgacaa gttaaatcaa
    20641 accatcaatg agaaaaacca agaaatcgat cagctgaagc aacaagatgg cacaaaagca
    20701 aaagaggaag taactgaagc acaaagaaag acggcagaag agttcggtca attggccatt
    20761 gataaatcat taagcagaaa tgaattaacg gaaaaactaa aagatgtcgc aacccaagaa
    20821 gtgatcgaca aattaagtcc tacagacgat gatcaccaac atgatgatta cggaagttac
    20881 tcctttacat taggtgacgt gcaaacttac gctcaaacaa gcagtgtaaa agaggaacaa
    20941 aattttgtcg tatttgttga ttatacaatc ggcaatccgc aattcaaaga tgtcaaacca
    21001 caaaaaataa aaggtggatt aactgtcact gaaaaacaag tagatggtca atggaaagtc
    21061 atagacttta gttatttcac tcgttaggag gaagaaaatg agtaagaaaa aaggagaaag
    21121 acaacgtttc aaaaaacgaa aaagttatct tagatggctg ggaatcttta tgattctcgg
    21181 ctttttgatt cttgctagtc cgatttatac gaatcagaag gtagatatac cagatgaggt
    21241 aagcacgaac gcctttttaa acacgaaaga gctgacaatg gtttcaaaag aaaaaggagc
    21301 gaaccagcaa ttagtttttg aatttgcgat cgatgatgaa gatcaaagct tgttaaaaga
    21361 actggctaat cttaattatc gcgtagaagt aaagacagct aaaggggatt atcaagcaat
    21421 acgagccaaa gtgattaagg tgtctgatga ttattttgca gtgaagctaa cgcatgtacc
    21481 agaagaatat atcgccatgc gtttaaccat tattcctgaa aaaattgatc caaaagtgga
    21541 tatgcaagaa ccacaagatc tgatttatta cgtgcacgaa gataaggtta aagatcaagt
    21601 gaaagatgaa gattatgaac aacacgcgat caattacaaa gtgaaaggat acaagaaaga
    21661 acaaaaagca gctcaaaaac aaatggaatc tttccaagca accattgatt tgaatgaaga
    21721 attagtgaag aagctaaaag atcaattgcc ttatcaagtc gcagatgatc aggaaaatac
    21781 tgaaaacaag attaatagct accagcaaga aattgaaaca tcgaaaacgc aactgaaaga
    21841 tcaagaagag atcattcaaa aacttcaaga aaaaattgat cgtatgacaa aagaaaagat
    21901 ttagaaggac aggtggagtg aaaaaatgaa gcgaacaaac ttggctgaca aagatacttt
    21961 cgctgagcag aaagtattga ttaaagccta tctcaaagtt gtaaaagcta aagaaatatg
    22021 tcgagaagtg aatttatcta acacaacaat atctgagttg aagaatggtc gaaagaattt
    22081 agatacttta aaattcagat catttgaagc tctatatgta gctgctaaaa aatattttac
    22141 agctgataac aaataaaaaa caggagtatt ttatgtgaaa aaagtatata aaaaatgctt
    22201 atgaatcagc tatatcacct taaccatttg ttaatgagat gggagaaaac attaaccacg
    22261 attctaacgc aaacatcaag cacataaaac atttaacact cctcctttct ggctgaagat
    22321 ttcaaccaaa caggaagaca gcgtatcaat tatagcatac actttttctt aaattcattt
    22381 ttcatatgac aaaatatttc cttctaatcg acaaagttag aaggattttt tattgtctaa
    22441 atacacaaat aaccttaatt tttaaagagc ataaggaaac gtgatatcac tttctcacga
    22501 aaggtgttag gtaggctgtg cctaccacgt ttctaagaaa gataaatgat atcaaaattt
    22561 tggatttgtg tgtagatttg tgggtaggat gaaagcgaat gtgggcagta aaataagcgt
    22621 ttgtgtgtag caaaatcaaa aaatgtgtag ggatatgtgt agctacacat atccctacac
    22681 ataataaatt tggaggaaaa tatggactta ttaattagaa atattgaaga aaagtatatt
    22741 aataaaattg acaaacgttg tgaagaactt acggtcaaga ctggcaaaaa atggagtcga
    22801 aatcaatacc taaaagtttt gatcgaaaat gattttgatc acgctcttct gaattataaa
    22861 aaagaccaat ttgatcggtt gttagaaaaa tttgtggata ttcaaactta taatacgaaa
    22921 actttagaag aatatattgc aacaaataat caattaattg gtttgttaat tgaataataa
    22981 aaatagtatt gtgaatttat aaaaggagga aatcaatttt ggcaaaagca acaagaaaac
    23041 atgtatatct ttacgatatg catatcgaag tacttgatga aataatttct actcatgaag
    23101 atatatctaa ttatagtgaa gcagtcagac atttgtgtat gatgtatgat ccaaaaatcg
    23161 aagaaaaaat aaatccaatt caaattaaat tgaatgcaat gggaaaagaa atttctattt
    23221 taaccgaaat ggtttcagag tttgcggatc ttcatttgaa agatacagga atactaattg
    23281 gtacaaattc aactgtttat caagaagcag aagaacgtgt ggaaggaaaa attaaatcca
    23341 atcaaaccaa aaaatattct tctaagttaa agaaaccagt gaatgaatct gttcatacca
    23401 gagtagatcc atttgatcca acgaagattc taaaaagata aaagtagaag gagaacgtga
    23461 tgaataaatc gcctgcagtt attcttactt gtcaatttac cttgcccaat aaaaaatctt
    23521 tttcaacata cattgattat atgacaagag aaaaagcact ggaagaaatg gaacgacgta
    23581 cgcccgaaga agaacaggaa ctgcaacgaa tcaagaatgc tttagaggat ttttatattc
    23641 ctgaaggaga aactttttcg gaaagtaaga cggataaaga attcagtgaa acgtcgaatg
    23701 aagctcaatc gttgatggaa catggaatca attttgacaa gttagaggaa caagatttta
    23761 ccaaatatct ttcttatatg acccgtcact atgcgttaga acaaaagaaa gaactgacgc
    23821 aaagggaaca aaaagaatac caacggttga accaagcaat gaaaaaatat caaacagagc
    23881 cacataaaaa agcttcagga aatcttcctg gagttttttc tatggcttca gacgaagtga
    23941 aaggaagtga tctgaaggag atcaaacaaa tcttccaaag aggacaacaa aaaggctcaa
    24001 tcatttatca agatgttatc tctcacgaca attcgtatct tgaaaaaatg ggcttgtacg
    24061 atcccaaaac agatacctta gatgaagaag gtttgaaagc agctggaaga aaaatgatgg
    24121 agacattatt cgagaaagaa caattgaatg aaacaggtta ctggatggca acgattcacc
    24181 gtaacactaa gcatatccat attcattttg cagttgttga aaaagaaaat acgcgtaaac
    24241 cgtatactga gattgaaaat ggtgtgagtt atatcgtacc gaaggggaag agaagacaag
    24301 caacattaga cgcaatgaaa accaattatg tacatgagtt agaacgtttc tcttatgaaa
    24361 aggatcgaat gatcggtatg gagaaacgaa atttattgac acgaaaaagt gatttgcgta
    24421 acaccttaac aaaagtagta aaagaaccag cacgttacga tcgacacgcc atgaacttat
    24481 taaatgaagt atataaaacc ctacctgaaa aacgttcgga atggaattat ggagatgaaa
    24541 atcgaaccaa gctttcacca tttacaaggg gaaaacttga tgagttaacc aaatatgttt
    24601 taacaaaaga acctggatat caagaatata tacagatttc aaaagcattg aaagaagaag
    24661 ccaaaagcct ttatggggaa agcaaaagag aaacaaagaa tgcagaaaaa aacgccctct
    24721 ttgatttaaa aaagcgcaca ggaaatgcga ttttaacaga actgaagaga caggatagtc
    24781 aaataaaacc attaatagaa agtgtcaatc agatgcaatc tatttacaat gaacgatcct
    24841 ttcgaacact caaacagttg ccaacaaatg aatacaagta tagttatcaa gaattcgtaa
    24901 agcgtaggaa acaattccaa caaagagttg tcacgcagcg aagcttgcgt caattaaatc
    24961 gaacaattaa tgcacatgaa gaaaaatacc acgcaagtaa agcatatgaa gagcaacagc
    25021 gaagaatcca aatggaacaa gaaagacaat aggaggaaga gaaatgaaac aatatttaga
    25081 tcaatggaaa gtcatagaag gatcactaaa agaagaacga atcgaacaac ttcctgattg
    25141 tttagaaaaa gagcatttgt ttcagataag cgaaatgttg agaaacgaac agtttgatcc
    25201 aaatcaattt ttagttgttg agtacccagc tactggtgtg tattgttgta atcatgtgaa
    25261 gggtgaaaaa tattttatta ttcaagaata cgaaggtaag ttagcgcctt attatactac
    25321 ttgggaaata aatgaagagg gaatcaataa tttcccatgt gaaagcattg aagagtctat
    25381 ttctctcacg gaatgttaaa taaatagaga cgtaaaagag tcctgaacgt gattgtccag
    25441 gactcttttt caatgaattg ttttccgcca attcactgag tggtgcgatt caattttatt
    25501 atatcaaaag aaagtgagat tccaaagtga agggaggaag aagaaatgac tcagacaacg
    25561 attggtgttg tggttgaaaa aagcagggat aatctcgttt ttgtgacaga aattcaaaca
    25621 ggtagagctt ttgtagtcac agacaaagct gcaaaagcgt atcaaaatgg caatatttta
    25681 acgttaaatc tgactacgaa gatgttcgtt gatgcagtag aaaattatcc atttgtttag
    25741 acaaacgata ttttacaagg aggaaacgga catggcagaa gaaaaagaac cactagataa
    25801 tagtcggttg ggaaagtcca aaagaaaatt agtacggttg caaaacgaat taaataatca
    25861 atttcaaaag ctatttgatc accaaaaatt gacaaatggg caaccaatga atgacaaacg
    25921 aaatggtcgt tcatggttta accagcaaga aaaaatagaa aataaaatac aaagtttgag
    25981 agaagagatc aagcaacaag aaaaacaggt agaaaaactt gaaagacaag aagagctaaa
    26041 ggaaatgggc tataacaagt acggcggact ggatatgacg atcgataata ttccaagaat
    26101 caaagaagaa atcgaacgct ttgaaaaagg tgaatcgact ttttcagccg ccacgatccg
    26161 aaaatatcaa cggaagttgg aaacattaga acagctgaaa gaaaaatcag aaaaaggaaa
    26221 agaaaatatc ctccctgaag tacaagcgat cattgacagc ggacgagtga cgcaatggaa
    26281 aaaaaatcca accatttatt ttttgaaagg ctatcgaaaa gttgctttag aactgaatga
    26341 aaacggggga tttgaggaat caaaaaaata ccaagccacg tcggaagagg aaaaagcagc
    26401 tgttcgtgaa ttacttgaag aggctgcacc aactgattct ctgaaggaga aagaaaaaga
    26461 tcatttctat cgagtagaat tcaacgaaaa tgatccagaa aaaggattga aaagttatgg
    26521 tggggagatc gtaacattag aattgatcga tcaacttaaa acgttggata accaatacgc
    26581 tgcagaagca ggttattata aattctattt tgaagaagtg aagaacggag aagtagtaga
    26641 tcagatccga atggatctag gggatggcgt caacagtaat caccccatct atttagagtt
    26701 agcaaaattg tgcgaacaag tacctgagaa ggaacaaaaa caatcggtca aaaatgaacc
    26761 tgtttcgaaa gaacaaatgc agtcgaaaga aacaaaaaca aaacaaaagg gctatcaaaa
    26821 acgaacggca gaagagatca aacaagaagt taaatccctt tcgcaacagg cactggatgc
    26881 ggtgaaaaag catacccatt cttctgaaga cgtcaaagaa cttttgaact ttatgtctcg
    26941 ttttccagaa cgatccttta gaaatcaatt attgattgag gaacaatttc caggtgcaac
    27001 ggcttgttta ggtcgtgccg cgttgaaaaa agaaggaatc tatatcaaaa aaggggaaca
    27061 agggaacaag atctttgtca gaaaaacggt caaaggattt tatgagaaag gacgtggctt
    27121 tgttcgagaa acagaagcta caccagaaga taaaaaacga atcgaaagtg gtcaaatcga
    27181 agtgatcaaa aaaccttact acacgattga aaaagtgttt gatatcacac aaacacagtt
    27241 aaaacctgaa gactatccga aaatctttcc gaaccgtgtc tttgattttc aattggataa
    27301 aaaaggacag tcagaacttc aagctgggat tcaagcggta gccaacaata ttggaataca
    27361 aattcgagat atgacagaaa gtgaagtcta tcaaagagaa ttaggacaag cacgtggggc
    27421 gtatgttcgt aacgaattta ctggcgaaga agaaatcgtc atgaatactc gaaatactcc
    27481 tacgcagcat ttagctacaa gtattcatga attagcccat gcaagaatgc acaaattttc
    27541 agaattagat accgcaacta aagaattgca ggcagaaatg acttcttata ttgtgtgcaa
    27601 acattttgga atggacacgt cggagaaagc tattccttat attgcagcat ggacgaagaa
    27661 tggtcagacg ttggatgata aggaagcggc ggaacgtggg aaaatcatga atgatgtcag
    27721 ccgtgtcgca aatgaattca ttcaaaccat ttctaatgaa atcaatcaat acagagaaag
    27781 agaaccagag atccaaccag cagaagtgaa gcaggagcat aaaaatactg tagaaacggc
    27841 agagaataag aatattgtgc ccgttggttc actttggatc gacaagaaag acgggaaagt
    27901 aatggaagaa gatacgggtc gaacgttaca tttgaaggat gcggcgttta ttgaagatcc
    27961 gcagcataat caagtgattt taaaaggaaa aagttatgga aaagagattc aaacggcgtt
    28021 accttcagaa gaatttgcgc taggaaatcc attccgattt tcacttgaaa cagtgtcaga
    28081 agtggccaaa cgaaaaaatg aaatctatta tgaacaacaa ttgtcacaaa aagaacgcgt
    28141 ataaaacaag taaaaaagac tatctgaaaa acggatagtc ttttttcttt tgggaggttt
    28201 gaaaatgacc gaaaaaaaaa tgagtcttat tgatcgatgt aaacagatcg atatagtgga
    28261 ttttgctcga aacaatggaa tggctgtagt caataaagga cgagactatc gcttagaaga
    28321 ccatgattca tttgtttttg atcgaagaaa acaacgtttt tattggaaca gtcaaaatat
    28381 tagtggcgac attattgaat tagctaaact ttttttcata gacaaagaga tccaagactc
    28441 taagcaacaa tttaaagcag ccactgattt tattctaaag aatgaagaca aaacagaacg
    28501 agtggaaaat cttcattttg aaacagaaaa gtacaaagat caccccgttg attatcaacc
    28561 actcacagaa aaaggaagga attatctgaa ggaagaacga aaactaccag agtggctgat
    28621 tgattatgca gaaaaagaag ggttgattgc cgaactaaag ccaaaatatg aacggcaaaa
    28681 tttcttagtt cgagacgatc gtttagatca tgcggtcgcc tttttatgga aagatccaca
    28741 gacaaaagag acagtagggg cgagttatca aggaacgttc attgattatg aacgttttgg
    28801 tgagagaggc acatataagc atatagataa aaattcgaca gcgaatcatg gctttaacct
    28861 aaagatcggt gaccctaagc agttaaaatt ttttgagagt agtattgatt tacttagcta
    28921 tgctgcactg aatcgtgatc agttaaacga tacgtggtta gtctctatgg aagggttaaa
    28981 acaccatgtg atcagtcatt attttggtga ggcagtatct gaattgagaa agaaacaagc
    29041 ctttcctcaa tccatcgaga tttgtgtgga taatgaccgt gcaggacata ttttctacga
    29101 aaaagaacaa ttaatgggcg cagtcgatcc atttacgaat cagaaagtac gttgtgaacg
    29161 aggaatcgct aatgattggc aagtgccaaa agaatacaaa atcatttatg aagaagtcgc
    29221 aaaagaagaa aaggtcacac cagaagctat tatggccata cacaagacag aaaacaatct
    29281 gcaattaacg aaccaactgg tttccgctca caaagtaaat gcgttttttg gccagcagct
    29341 ttctgttaac gattcaatag aagccattaa tttgaaagat atttgtagag aagtcgcaaa
    29401 agaattaaaa gtttgtgaac gcgtggatgg tacgtacgat tttgatcgtt tttatcaaga
    29461 aaaaggggat attaatgcgc agattctttt ctcttataaa gcagaacaat actacaaagg
    29521 ctataaaaat catgaacatg aatttatacc tgaagtaaaa aaggactgga acgatcaatt
    29581 gaagcatgag atccagcaac aagaaatcag aaagcaaaaa cgtgccatgt tgtttcaaca
    29641 aggcagacaa caagaaaggg agtgaaatcg tcatgatgaa aaccgttatt ttagcagaaa
    29701 agccgtcaca agcgaaagct tatgccgata gcttctctaa agccactcga aaagacgggt
    29761 attttgaaat ccaagatcga ttgtttcctg gtgaaactgt gatcacgtat ggttttggtc
    29821 atttagtcga attagattcg cccgatatgt acgatgaaaa ttggaaacaa tggtcattag
    29881 aacatttgcc tattttccct acacattatc actatcatgt tcctaaagat aagaaaaagc
    29941 aattcaaggt ggtgaaacag caacttcaat cggccgacac cattattatt gcgacagata
    30001 gcgatcgtga aggggaactg atcgcttgga cgatcattca acaagtaggc gccgatcaag
    30061 gaaaaacctt taaaagactt tggatcaact ccttagaaaa agaagccatt tatcagggat
    30121 tccagcagtt acgagacgca aaagagacct atccaaaatt tgaagaagca caagcacgtc
    30181 agatcgccga ttggttaatt gggatgaacg gtagcccact gtatagttta ctcttgcagc
    30241 aaaaaggcat accaggaagc ttttccttag gcagagtcca aaccccaacg ctttacatga
    30301 tttatcaatt gcaggaaaag attagaaatt tcaagaaaga accttatttt gaaggaaaat
    30361 ccgaagtaat cgctcaaaat ggcgcatttg atgcgaagct tgatccgaat gaaacccaag
    30421 cgacgcaaga agcatttgaa gattatttaa aagaaaaggg ggttcaattg ggaaaacagc
    30481 caggcatgat tcgccaagtt gaaaccgaga aaaaaagcgc ggcaagtcca cgtctttttt
    30541 ctctatccag ccttcagtct aaaatgaatc agctcatgaa agctagtgcc aaagacactt
    30601 tggaagccat gcaaggcttg tatgaaggga aatatctaag ctatccacga acagacaccc
    30661 cttatattac ggaaggggag tatgcgtatt tgttggatca tttagatgaa tacaagcatt
    30721 ttttaaaggc cgaatcaatc ccaaccccta ttcatacgcc gaatagtcgg tatgtgaata
    30781 acaagaaagt ccaggaacat tacgcgatca ttcctacaaa aacggttatg acagcagcgg
    30841 cttttgaaca actaagtcca ctgcagcagg ccatttatga acaagtgtta aaaacaacgg
    30901 tggccatgtt tgcggaaaaa tatacctatg aagaaacaac gatcttgacg caagtccagc
    30961 agcttcagtt aaaggcaatc ggaaaagtac cgatagatct aggttggaag aagttatttg
    31021 gcaaagagaa cgacggaaaa gagaaggaag aagagccgct acttcctaaa gtgacaaaag
    31081 gagaaatggt gacggtagat ctacaagtcc ttgaaaaaga aaccaagcca ccacagcctt
    31141 ataccgaagg tacgttgatc acggcgatga aaacagctgg gaaaaccgtt gatagtgaag
    31201 aagcgcaatc catattgaaa gaagtggaag gaatcggtac agaagcgaca cgggcgaata
    31261 ttattgaaac cttgaaacaa aaggaatata tcaaggtaga gaaaaataag ctcgtggtga
    31321 cgaataaagg gattttgctt tgccaagccg tggaaaaaga gccgctgctt acgagtgccg
    31381 aaatgaccgc caaatgggaa agctacttgt taaagatcgg tgaacgaaaa ggaacacaaa
    31441 ccacttttct agccaatatc caaaaatttg tctctcactt attggaagtc gtaccagggc
    31501 aaatccaatc cactgatttt ggatcgacgt tacaagaagt gaaagcagcc agtgaaaagc
    31561 aagaagccgc gcgtcattta ggaatttgtc caaaatgcca ggaacaagaa gtcttactct
    31621 atcaaaaggt ggcagcatgt acgtctgaag cctgtgattt caaactatgg acaacgattg
    31681 ccaaaaagaa acttacagcg acacagctga aagagatcat tcaaaatagc cgaacaagcc
    31741 aaccagtgaa agggttaaaa ggtcagaagg gttcgtttga agcaacgatt gttttaaaag
    31801 aagacttcac tacaggcttt gaattttctg aaaagaaaaa aacgaacttc aagaaaagaa
    31861 cacgacgcac tacaaaataa tggagggtta atcttatgcg taatacaaca cacaaacaac
    31921 aagaaatcga aaatcaaaaa gccaaaattc gttcagaaca ggaaaaaatg cgtgccagca
    31981 aacgaagact gcagcaattg aagaaccagc agcggattca aaaagaaatc gaattagatc
    32041 gataaattcc tttcttgtta atataaaact aacatgttat agttgtgtta gttttatatt
    32101 aactttaggg gaagagtaat gaaagacaaa atccaatgta tttttgataa acataatggc
    32161 aacctatcct tgaaggatgc tagaagtgaa ggattatcgc ctatgacgtt agatcgatta
    32221 gccgaaaagg ataaaataga aaaggtagca ccaggtttct ataccttaaa ggatgcgttc
    32281 gtggatgaat tgtatctcgc tcagagtatc tattccagag ggattttttc tcatgagact
    32341 gcgctcgatc tttttcaagt aggaacatat atccctaaaa agatcaatat gatgtttcct
    32401 aaaggataca atgtatcaaa ggagaaatta gatcagtatg ccattcaacc gcattacact
    32461 aataaagaaa attttgacct gggagtcaca gaaacagaaa gcttttacgg caataaaatt
    32521 ttagtctatg atttagagcg aacgttatgt gacatgtgga atccaagata caaagcaagc
    32581 gtagaggtaa aacaagaagc attaaaagaa tatatgacaa gtaccagtag aaatacaaat
    32641 aagctaagaa gatacatgga tattttaaaa tcaccaaaag aaatgacttt gtatatgttg
    32701 ccactttatt gagaggagta aagatgaatg acaccgacac aatttaaaga gaaaacaaga
    32761 aatttcgcag cagagaaaca tttaaaccca caaattgtcc aaagatatta tatgatggaa
    32821 aaattattag aatgtattgt tgaatcgcct tatcatgatg attttgtatt aaaaggggga
    32881 tttctgattg gatcaaaata cgggttagaa aaccgttcaa ccgttgatat tgatacgact
    32941 tatcgaaatt cgaaactaac aaaagaaaag ttagaaactg ttttttctga attaacagct
    33001 aatcctacaa aagatggaat tcgttttgaa ttaaagggat taacagagac gagggaagcc
    33061 gattattatc caggttttca agcaaagttt attgcttatc ttgaaaatgc acgtgtacct
    33121 tttaaattag atattacttc aggtgattct attattccag cagcattgat gtatgaacac
    33181 aaattaatgt ttgaagataa aacgattcat attccagctt atcctacgga acaaattcta
    33241 gctgaaaaat tacatgccac gtttagtttt ggaaaagata attcgcgcat gaaagatttt
    33301 tatgacctgt atatgattcc gaaattagaa cgtatcgatg agaaaaattt gtataagagt
    33361 gttcaaggaa cattcaagga aagaaataat caatcggtgt ttaagcattt gtatgatcag
    33421 gaaatgcctc aattggtgaa agatgagggc ttgagagaaa agtggaaaag ataccaacaa
    33481 gaaaactatt ttgccaaaga catttctttc gaatctacac taacttccgt tgattccctg
    33541 atgcaatcaa ttgtagagca agagaaggca gaacgaaaac aaaattttaa gagatcacaa
    33601 caaatgaatc aagaaatgga gagataaaga tgatcaataa tgtaacttta gtagggcgat
    33661 taactaaaga tccagattta cgttataccg caagtggcac agcagtggcg actttttccc
    33721 tagccgttaa tcgtaatttc acgaatcaaa acggcaatcg agaagctgat tttatcaatt
    33781 gcgtgatttg gcgcaaacca gcagaaacga tggcgacatt agctaaaaaa ggcagcttga
    33841 tcggcgttgt cggacgaatt caaacacgga cttatgacaa ccagcaagga caacgtgtgt
    33901 atgtgacaga agttgtggcc gataacttcc aactattaga aagtaaagca gcgacagaaa
    33961 gccgtatgca cgctgatcaa tcgtccagca gtccaagccc taccaccttt gaacaacggg
    34021 atacagcgac gcctaacaac aatggattga acgcttcaca gaatccattt ggtggacaat
    34081 caatcgatat tagcgatgat gatctaccgt tctaagtagc attttctatt ggttgcattt
    34141 gctacggttt atgttataat ttagacaaca aaaagaattt tcaatctata accaaaaacc
    34201 actagctggc gggctagtgg ctttctttta cgtttctatt ataagaatta acgggtaggc
    34261 ttactgtctt tcttggcaaa tttacaatca agccatttgc agatatagtg cgcggctact
    34321 tctgccatga ttgcaaaaac aaaatcaaaa agaattttca aacctataac ctcctcccct
    34381 tgccagatta ggccggggta gcagacagca agactattat agcataattg aaatatccaa
    34441 atgattggat ttttgctata attttagcga ggtgattgtg atgaacaaat atgttggttt
    34501 gcttgataaa atccgagtaa taaaaacgca accgttattg gttcgcttta ctttacaaac
    34561 gattcatgaa tcgattaatt gtgtagttgc agatattgaa attatcgata aattgctaat
    34621 tatggatgat ggaaaataca atattgccgt cacaggtcac ttcaataaac gaaatcagct
    34681 agttattgct agcatgtatg ttagaaatcc cgatcatttt acgcgatcga tgggcatata
    34741 aataaaagaa gagttgtagt cctaaatgag gattgcagct ctttttgttt tacatagaaa
    34801 caggaaaaga cctttttagg aagacacaag tgccttctca attgaaaaac ctttctctca
    34861 ctccaaccgc caccgccgtt cgagaagaaa ggaagtgaac gctgcacgtt cactaagata
    34921 tggtgtcttg caaagcaaga caaaatagac aaaacttcct ctcaccaaaa ggttcgttcc
    34981 tgttcgttat ctctttattg atttgccaga ccggcaacaa gttgcctttt atcaccaaaa
    35041 agcgacaagg acaagggtaa atggcacgct gcgcgccctt gtccttgtcg cttttcgttg
    35101 atttattctg gccaacaaat caataaagaa agaactaacg aaaggaagta aaacctttcc
    35161 taagaggagg cagaatcaaa catgcgaaag aaatttgatt attggggtgt tcccttcagc
    35221 gaattattcc caaactatca tgcaccacat actgttgaat gcgattgtgg agaaagagcg
    35281 aaatgtatca aaagctatcg tttgtatcaa tgtccaacat gcgggaaaaa atataccttg
    35341 tcttatggcg attacatttt gattgatgag aaaggaaaga aacgctgatg aaaacacaac
    35401 acaaattatt gactgtaaaa gaagtcgttc aaattttggt ggacaaagaa gtcatttctg
    35461 aaaaagtagc agaagcttta cttattccgc ggtactggac gatagaaacc cctttagtag
    35521 aggatgcacc gactatgcta tttgaagacc tagtgaagat tgcaggatat gaactgcatt
    35581 atcaagaaaa catggaaact tatgaaatcg aatattggtt aaccaaatta gagaactaaa
    35641 aaggagaaaa aatatggctc aaaattacta tgacgaattt gtcaaattac ctttggataa
    35701 aatggcacaa aaaatggagg atatgacgtt cctataccat gaaacacgtg tacccaaaaa
    35761 acattacaag gaaaaattgt cagtcgcagt ggaagaaatg attgaatcag gtgtcgaaat
    35821 gaatctcatt gcgacgtact atcgaacact ggaagaactg aaaaaacaaa atggaaaatg
    35881 gtttttccaa gccttactat gcttggaagt aggtgtcaaa ccaagcacta tcaaaccttc
    35941 cgaatatcaa gccttagaac tgacttatgc aaaattcgca cagattaaga aagctaaagc
    36001 aatggctagc gactggctag atacttttga aagtatcgaa aaaaatggtg cattttatac
    36061 gattgaatcg gaggagaaag ataatgaata aggtggttta tcagctaaca acagaagatg
    36121 tttcgcaatt tttaacaagc aaagaacact tgaatgaagt aatcagacgg attcaaaaag
    36181 actttagttg tatggaagaa atgaagtact ttattcaaag agtggaagaa cagttgtata
    36241 tggaaagcga aacaagtcaa acgaaggtca cagtcagcca taatcattta gacgtgtata
    36301 aacgtcgtcc tgaaacggta aaaaaaggaa cagagcttct acgactagaa acagaaaaat
    36361 acatcatgaa tgtgtaccta gaaaggagtg agctggtcga aaactttgtc agctacacga
    36421 tttgtaggaa acactattac tcagaatcag aatattattc aattttcgag agcatggaat
    36481 taatggaaga agaaatggaa gagctaaaca gcaaaaatgc gacggtagac gattattacc
    36541 ttgtatttac taggtttttg acgtgtggga cggaatctat cgtgttaatg ggaatggatt
    36601 atgaactcgc tgtacccgaa gcatttttga gggaatatgt agaagaagta gataacccag
    36661 gcgacgactg gtacacgact ttaactgatt ttttagacac ctacacttac gacgatacag
    36721 aagaaatcca aaatgaactt gaccgtaaac gaatgccata tcaacgattt gaaaggaaaa
    36781 aaggtaaata accatttata ccgaaaagtg aggatgtgtg aaataagaac aaaaaacctt
    36841 gaaattaagg gaatggttct tatttcgcac caatcgctcc aaccgccccc gccgcttagg
    36901 tggtgcgaaa taagcgggca aagcccgata acctaagaga aagaaggaga tccatatgaa
    36961 taaaatcatt cctctattgt tcgtactgtt tgttggcgag atacttatgc gtcatgctgc
    37021 agctgtacat ttaaacttcg tagtattctt aggaatactg gtattttgtt tgatcttttg
    37081 gcgatttgta ttagcccttg tgagtctact attcaagatg attgcagcca tatttttcat
    37141 agggttattt cgatgaaagg agagagtact aatgccaatt gcaataggaa ataaacggtt
    37201 acccgtgaca ttagatgaaa agagacaaaa agaattgcag caactaaagc agaagtacgg
    37261 aaaaagtgaa tctaagatta tgtgtattgc gttagatcta ttgatcgcgc aagaaaaagc
    37321 aggatttgat ataccagcac taaaaaagtg acgtcacttt tcatttgaag aaacaaaagt
    37381 gatagcactt aattgttcct actatttaga atattttcta tgtccttttt attccatttt
    37441 gtatcaataa ctatgaaatc tttaaagtat ccagaagaga ttatttttag atcaattttt
    37501 ttcttacctg attctaaaga ttttgttttc ttaaacatat tatgatttcc tttcgactat
    37561 tttcatataa atgttaacat agtttatgga ttcatggaat gttaatctaa tttaatagaa
    37621 aaggataatt aagtatatac gttttttctt atgtatgtta tactatgtat atacagaaag
    37681 aggtgtctac tatggaaatg ttacctataa aaaaatgggg aaatagtaat ggccttagat
    37741 tgccaaagta tattatggaa tatcttggaa ttcatacaga agacaaagtg aagataatac
    37801 aagaagaagt aaacggtcag aagcggctaa ttatcgaagc ggctaatata gaaaatgaat
    37861 taacaattga acagttattt gaaaattaca ctgatgaaag aaatcatgtg acgattcaaa
    37921 atttaggtga agcagtaggt aatgaaaaat ggtgagaaaa cctagacaag gcgatattct
    37981 attattgaat acagcacctc gatctggaca cgaacagaca ggtaaaagac cttatattgt
    38041 tttaagtcat gatattattg cggattatag taacgttgtt acagttgctc caatttctag
    38101 tacaactaga aactatccgc tatatgtaaa tataaactca gaatatgaaa tgaagacaac
    38161 aggaaaagta ttattagatc aactaacgac aattgattat gaagccagag agtgtatttt
    38221 tttggaaaaa gcgcatgata gtttagttga ggagttattg atgaaagtta gaacagtttt
    38281 tcaaaaagta agcaaataaa atagaaacag cagcatgatc aactattcgt gattgaccat
    38341 gctgtctttt tttattctat atacggtttg tctcgtaaca ttgtagtctt ttgcaatctt
    38401 agcgatcgca acaccttttt ttagatcctc aacgatgttt ttataaacaa gacgacgttg
    38461 gggatctttt gcattggcac tgtatagctt aggacgtcct ttataaacac cgttcgcttt
    38521 tgcaatctta atgccttgtg cctgacgtcg tttagattcg gtacgttctt gttcagcgat
    38581 catggccaaa atttgaatga tcaaatcttt aatgaattta tccaggagag gattgccaat
    38641 tgcttcggcc atgatcggta gacttgtgat gatcagctgc acatttttct tcttcaagta
    38701 attgactgaa gcgatgatct cgtcgtaatt tcttccaagt cgatcaatag cttccacaat
    38761 aaaaatatct tgatctcgga cgaaatccag tgcttcttga aaaacaagtc gatgagtaat
    38821 cgttgcgcca gattgtttct caatgaaaat tctctctgca ccgaattttt tcatttcttc
    38881 aatctgccgt tgttcatttt gatcgatgga tgagacacgt atgtatgcaa ctctcaaaat
    38941 ttttacctac tttctgcaac tatttaaaaa acaccctaat catacacgag aaaaccagat
    39001 ttttcaatgt cgcggtcgtg tctagttagg gtatacctta tatagacaaa aactaatgat
    39061 ggaggaaata gtatgtgtca cttttctttt taaatggcaa tattttttgt aaataaatgt
    39121 caatcaagaa aatttaaacg attatgacat taaaaatata ttttatgaat tttctagtat
    39181 ataacttgat ttatctgtta agatttatta agaaaac
//
LOCUS       DACDUU010000021        37139 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.021, whole genome shotgun sequence.
ACCESSION   DACDUU010000021 DACDUU010000000
VERSION     DACDUU010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.021"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..334)
                     /locus_tag="IWL30_001090"
     CDS             complement(<1..334)
                     /locus_tag="IWL30_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705873.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFE"
     gene            complement(509..2644)
                     /locus_tag="IWL30_001091"
     CDS             complement(509..2644)
                     /locus_tag="IWL30_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9705874.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(2727..4382)
                     /locus_tag="IWL30_001092"
     CDS             complement(2727..4382)
                     /locus_tag="IWL30_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9705875.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(4384..4698)
                     /locus_tag="IWL30_001093"
     CDS             complement(4384..4698)
                     /locus_tag="IWL30_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP9705876.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            4868..5128
                     /locus_tag="IWL30_001094"
     CDS             4868..5128
                     /locus_tag="IWL30_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAP9705877.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            5142..5456
                     /locus_tag="IWL30_001095"
     CDS             5142..5456
                     /locus_tag="IWL30_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAP9705878.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(6186..6740)
                     /locus_tag="IWL30_001096"
     CDS             complement(6186..6740)
                     /locus_tag="IWL30_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAP9705879.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(6866..8795)
                     /locus_tag="IWL30_001097"
                     /pseudo
     CDS             complement(6866..8795)
                     /locus_tag="IWL30_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(9101..9817)
                     /locus_tag="IWL30_001098"
     CDS             complement(9101..9817)
                     /locus_tag="IWL30_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP9705880.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(9818..11641)
                     /gene="glpO"
                     /locus_tag="IWL30_001099"
     CDS             complement(9818..11641)
                     /gene="glpO"
                     /locus_tag="IWL30_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAP9705881.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(11655..13151)
                     /gene="glpK"
                     /locus_tag="IWL30_001100"
     CDS             complement(11655..13151)
                     /gene="glpK"
                     /locus_tag="IWL30_001100"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAP9705882.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(13287..14747)
                     /locus_tag="IWL30_001101"
     CDS             complement(13287..14747)
                     /locus_tag="IWL30_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9705883.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(14737..16032)
                     /locus_tag="IWL30_001102"
     CDS             complement(14737..16032)
                     /locus_tag="IWL30_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9705884.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(16364..17041)
                     /locus_tag="IWL30_001103"
     CDS             complement(16364..17041)
                     /locus_tag="IWL30_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAP9705885.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(17071..18162)
                     /locus_tag="IWL30_001104"
     CDS             complement(17071..18162)
                     /locus_tag="IWL30_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAP9705886.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(18263..18697)
                     /locus_tag="IWL30_001105"
     CDS             complement(18263..18697)
                     /locus_tag="IWL30_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9705887.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(19281..20738)
                     /gene="ascB"
                     /locus_tag="IWL30_001106"
     CDS             complement(19281..20738)
                     /gene="ascB"
                     /locus_tag="IWL30_001106"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP9705888.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(20735..22630)
                     /locus_tag="IWL30_001107"
     CDS             complement(20735..22630)
                     /locus_tag="IWL30_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9705889.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(22688..24463)
                     /locus_tag="IWL30_001108"
     CDS             complement(22688..24463)
                     /locus_tag="IWL30_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9705890.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(24513..26009)
                     /locus_tag="IWL30_001109"
     CDS             complement(24513..26009)
                     /locus_tag="IWL30_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9705891.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(26203..27825)
                     /locus_tag="IWL30_001110"
     CDS             complement(26203..27825)
                     /locus_tag="IWL30_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP9705892.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(28153..29466)
                     /locus_tag="IWL30_001111"
     CDS             complement(28153..29466)
                     /locus_tag="IWL30_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP9705893.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(29711..29935)
                     /locus_tag="IWL30_001112"
     CDS             complement(29711..29935)
                     /locus_tag="IWL30_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705894.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(29955..30323)
                     /locus_tag="IWL30_001113"
     CDS             complement(29955..30323)
                     /locus_tag="IWL30_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP9705895.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(30519..31229)
                     /locus_tag="IWL30_001114"
     CDS             complement(30519..31229)
                     /locus_tag="IWL30_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705896.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(31247..31744)
                     /locus_tag="IWL30_001115"
     CDS             complement(31247..31744)
                     /locus_tag="IWL30_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9705897.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(31781..32233)
                     /locus_tag="IWL30_001116"
     CDS             complement(31781..32233)
                     /locus_tag="IWL30_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9705898.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(32368..32973)
                     /locus_tag="IWL30_001117"
     CDS             complement(32368..32973)
                     /locus_tag="IWL30_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP9705899.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(33423..34043)
                     /locus_tag="IWL30_001118"
     CDS             complement(33423..34043)
                     /locus_tag="IWL30_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9705900.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(34045..34914)
                     /locus_tag="IWL30_001119"
     CDS             complement(34045..34914)
                     /locus_tag="IWL30_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAP9705901.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(34937..35344)
                     /locus_tag="IWL30_001120"
     CDS             complement(34937..35344)
                     /locus_tag="IWL30_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP9705902.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(35363..36652)
                     /locus_tag="IWL30_001121"
     CDS             complement(35363..36652)
                     /locus_tag="IWL30_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9705903.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            36710..>37139
                     /locus_tag="IWL30_001122"
     CDS             36710..>37139
                     /locus_tag="IWL30_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9705904.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT        10605 a         7924 c         6207 g        12403 t
ORIGIN      
        1 attcaaaaga tttatttact tttttgtata gatttcgatt gttccaccta gtcaatacaa
       61 atgaggtaat caggaaactg aaaaaaatca atacaaaaaa gccgattaag aaatacagca
      121 ttttcttttc tgataaaaaa gcgtacaagg aaaaatcctt ggctctttct ggtctcgtta
      181 atgggaaaaa gacccatata aaaggaagaa caacgatcat ccatcctaaa agagccacta
      241 acgtttgcca aatctttaac cagtaattac cttttgaaaa atacttatct ttcaccaaaa
      301 ttgatgcaac ttcttctctt gttacttttt tcatatcctc acctcacgtt tttttgttac
      361 gacgtaggag tttaccatac atttcatttc ttttcacata aagtgttcct aaatttttcc
      421 taagaaaaag aacaaaacat ttctcatcag caaatgaaaa atattttgtt ctttttatca
      481 gttgttcttt ttatttttta tctcttaatt atgttgctct ttccaatacg acaatgcttt
      541 tcattcctct agatatcgcc gtgtacattt gtctgcgtct tctttgctca tctccatcgt
      601 tttcaaatgg atataagatt acatgatcaa attccagtcc ttttgctaga ctcaaaggaa
      661 tgatttggca gttttctaat ccatatgaat tttttttaat cgattcttgt agcgtttggg
      721 tttcctcttc tgtttgggtg ataactgccg ctttttctcc ttttagagaa accaaaatct
      781 cagctagtat tttcaacaat tcttcagagg atcgaaaaga gcggtattcg ggttcttttc
      841 cttgcggtcg gatcgatacg atgtcgatct tctcttgatc cacagcatag gttttgaaaa
      901 gctctgtgat tgctccgcta gaacggtaac tatttctaag atcataccga tggatcggca
      961 gatggtagtc gtcgaaacaa cgcataatat cagcaaaagt agtggaagaa ttaaagattg
     1021 cctgattctc gtcacctact aaagtaaagt gagtttttgg aaataattcg atcaatagac
     1081 ctaattgtgc cagggtataa tcttgtactt catcaatcaa tagatagcga aatgctggta
     1141 catttacctt ttcaacaagt aaatgacgaa tagctaatat aatgactgct tcatcaacag
     1201 tacgtgtggg ttgccaagca taagctgagc catatcgttt ttcataaatt ctttcaaaca
     1261 actgatgctc atccacccat gccatttctt cgatttgtct ggttattttt cgatattttt
     1321 tttgtaaaag tttccttgca taagctacaa tactttgttc cgaatcatca gaaatcaatt
     1381 taccgaataa ttccaactgc ctatcttctg tcaacgagag tacctgattc tgcacagcag
     1441 gactttttgc ttgtttcaaa agatgattct tccattcgct catcagtgcc tttttcgttg
     1501 cttgaagcct atcgatcaat ttaggatgag caggaacttt ctcatatatt ttttcgatcg
     1561 tttttttaga aaggactgtt tttccatttt tagtaagtcc acggataaaa ttcggatgat
     1621 ctaaaaacaa aggatctgat tgttttagat tatcgataaa ctttttgcta cgcaaacgtt
     1681 ctgtctgctc cgatacattt tctcccgtga tacgtttgaa ataagcctct tcaccttcta
     1741 tttcctctgc tgaaagctgt gaaaccagct gtatgatggt tagattcagt ggatttcttt
     1801 cacctaatga tggtaggaca ttcgaaatat agtcgataaa ccgatgatta ggtgatagaa
     1861 tcagcaagtc atcggatgtc atcgtatctc gatataaata gagcagatac gcaatacgct
     1921 gcatgatagc agaggtttta ccacttcctg ctacgccatt taccaagata ttttgacttt
     1981 tcacatcccg aatgattgtg ttttgttccg attggatcgt agcagtgata gccttcattt
     2041 cattggtctc atcttggcct aagacttcta ataatacgtc atcttgaata gctacagacg
     2101 tatcaaaaaa cttgaccaaa cgatctcttt ccaaaagaaa ttgtcgtctt tctttaataa
     2161 ccgtatcgat tttattttga cgaaccgtat aagaagatgg accaatcatg ttattgtaaa
     2221 aaagttcggc aattggtgaa cgccaatcat aaacaagatt ttcatctgca tcatctgtga
     2281 aatttgctgt accgatataa aaggattctt ttggctcatt atccagaaag tctacaacga
     2341 cttttccaaa gtagggactt ttcaataaac gttcgatctt tgctagctga ttttctgcgt
     2401 ttttctgttt gatattcatc tgatcgattt ctctattttt catttcaatc atcgaaaaag
     2461 tatccaggtt atctaagtaa ctgtcaaaat tcaatcgaag ctcgccagcc atttctttca
     2521 acgactggtt tccttctatt tttgccgtat ccatcgattc ccgcagttcg ttttttttat
     2581 caattagctt ttgatacacg ttcttcagat atgcttcttc taactgtttt tgtgtgttga
     2641 ccatacacat tctcctcttt ttttaaagtg gtggaaccga ttagtttacc tcttttaaga
     2701 gtcgaatgca agctttttaa cgagtatcat ttgttaggat atatcagttc ttcaggtcgg
     2761 acagtttgat ataaagaaaa tgcaccatca aggtttttta cgtggtagcc agactgcttc
     2821 aagattcttt ccgctagata gctacgtaat ccgctatgac agctgacgat atattcttgt
     2881 tggctgtcta attcatttaa tcggtcccga agttcattca aagggatatg tttggcttcc
     2941 ttgaatttgc ccttttccaa ttcttctgct gttcgcacat ctaataaaat ttttcctttt
     3001 gctagttctg tcggcaattc atgccattgt acggtttcac taagcccttc tgcaatgttc
     3061 attgctgcat atcctagcat gtttactgga tctttggctg agccaaatgg cggtgcatac
     3121 gtcaattcta attccggtaa atcgaagatc gtcaaatgcc ctttgattgc tgttgctaaa
     3181 atatcgatcc gtttatccac accttttgcc ccaacacctt gtgcaccata aatctcgcca
     3241 gtctctggat ggaagatcaa cttcagtaaa atatctgttg cttctggata atagcttgca
     3301 tgatcttttc ctgatacatg aatgacttca aaagacagtt cgttttcttt ggctgttctt
     3361 tcacttaaac cggtggatgc ggcagtcatt ccaaacgttc gtataattgc tgttccaata
     3421 ccgccttgat ttcttcgaga aattccggaa atcgtgtccg caacttgtct accttgacga
     3481 ttggctggtg aagcaagaga aataagtgca tcttttccag tgatttgctg ttttacaacg
     3541 atagcatccc ccaccgcata aatatcagga atattcgttt gataacgttc gtctactagg
     3601 atacccccgc gtatgcctaa ttcgattcca gcggtcactg ctagctggtt atcaggacga
     3661 acaccaatgg ataaaataac taaatcagca gggatggtac gcccatcatc cagtcgcaat
     3721 cgtttcccgt ggtcttcaaa tccagcgaca gcatgagaag taatcacttc tactccttga
     3781 tgagacaatt ctttttctat aaaagctgcc atttcttcat ccagtgttgg aagaatatgt
     3841 ggtgcttgtt cgatcaccat gacttctaaa cctcttcttt ttaggttttc tgccatttcc
     3901 aatccgatga atcctgcacc aacgatcacg gcacgctttg gctgtttttc taatgcatgc
     3961 atcactttat cgatatctgg tacatttcta atagaaaaaa cattgtcggc ttctgttatc
     4021 cctggaatcg atggaacaac tggttttgca ccaggagata aaaccaatgc atcgtactgt
     4081 tcaatgtgtt tttgaccctc atgtacgact tctaccgttt tgttttcggg aaagatggct
     4141 gtcacttcat gatttggacg cacatctaaa caaaaacgag cggataatga ttctggcgtt
     4201 tggacaagaa gattttcccg ttcactgatt tctcccgaaa gataataagg caagccgcaa
     4261 tttgcaaaag aaacatacgg tcctttttca aaaacaacga tttctgcatc ttccattaat
     4321 cggcgaagcc gtgtagcagc agacattcca cctgctacac ctccgacaat aacgattttc
     4381 atcttatttc cctcctactg ttcgatcaaa ccattgattc atgccaccac gtacattcac
     4441 tacatcataa ccagatttct ttaattcttt agctgcttgt ttactcctca tccctgattg
     4501 acaaatcaca tagacggtct tttctttgtc tccatcatat ctgtttatac tttgtaatgg
     4561 aacattttta gctcctttta tatgcccgcc ccggtattct gcaggtgttc taacatcaag
     4621 taacgctgga ttttccaaaa gcttttcctt taattctggg actgttatcg attttattga
     4681 ttgaaatagc aaattcattt tctgcacctc atttacattt ttataaatac ctcatggggt
     4741 atattaaaca ggagctttat taatatgtca accaaaatga ctcttcattt ttttcactac
     4801 tttttatatt tgcataaaat accgataagg ggtattatct aattataaaa atagaggaga
     4861 atgtgttatg gagattgata ataaaaatat cctgaaccgt ttgaaacgta cagagggaca
     4921 aattcgtggt atccaaaaaa tgatcgaaga cgaaaaagaa tgtatggatg tcatcactca
     4981 attaagcgct gttcgttcta gtattgatcg cgtaatgggc atgatcgttg cagataatct
     5041 gaaaaattgc tttgagaaac ctgatagtaa tccagaagaa caagcggcta aactagaaca
     5101 agcgatcaag atgattatta aaaagtaaag gaatgatttc aatgaaagtc gttttccata
     5161 ttgatgaatt agaaaaatgg tcagaaacag ggaaaaacgt aaaaaacctg ataaaagctt
     5221 ctccagaaac gacgattgtc gtatctgtaa atggcatcgc gattaccggt tatctagact
     5281 ctgccaatgc agaattctta gatttgcaag gagtcgtttt tcacgcttgc gccaatgcta
     5341 tgcgtgctaa tcatatttca gaaagttctt tacctgaaca agtaatcgtt gtaccagcag
     5401 gtgtccttga tttagttgaa ctgcaatccc aaggttatgc ttatatcaag ccttagtcag
     5461 tagaggttgc gacagaagtg atcagcttca agaaatttcg gcttattttt cgaagaagct
     5521 acttctggaa aaccgtgaat caaaggttgt gaaaacgctg tcctgcatca agtaataaga
     5581 acagccaaac aaaaatagct tctcatattt ttcgtttggt tgggcttatt accgcagatg
     5641 caagcttttg aacaccgttt aaggaagagg ttgtgaaatc agcggtttga cctaagtatt
     5701 agagaaaaaa acgggaaaat agctcctcat attttgccgt tttttgagct aataccgaag
     5761 gtcagctgat tgaacaccgt ttaaggaaga gattgtgaaa aagtcgctta gtttcgagta
     5821 ttaagaaaaa ttttgtgaaa atggatttct acatttttgc ataaatttgg cttaataccg
     5881 agaaactggc ttttgaatac cgtttaagga agaggttgta aaatcagcgg tttgacctga
     5941 gtattagaga aacaaacgga aaaatagctg ctcatatttt gccgtttttt gagctaatac
     6001 cgaaggtcag ctgattgaac accgtttacc aagaggttgt gagcctacca tttaactccg
     6061 aatcacaaga aataatttca aaaacaactt ctcctgtttt tggttcggtt ggcttattat
     6121 cgagaagtag ctactgggag tctatttatc aaaaaaagga atgcgacata ggtcacaccc
     6181 cttttttatt ttctttgaaa catctttcgg tactcgacag catacaagac aaaagattgt
     6241 aaaagaccgt aagtgatgac ggcaaaaatg actctcaccc acagcgttgg taattctggg
     6301 aaaagcagca aaaggatcat gccaacatag atgacagcag tcgctacttt gccataccac
     6361 ttcgctccac ttaatttgat atttttagat agcccgatta atccagctac aaacataaag
     6421 ccttctttta tcaagaatag accaaacaac agccaaagcc acggtcgata ccatgccata
     6481 gacaaaatca gaaaaagttg tgtcaattta tctgcaaacg gatcaagcac cttccctaag
     6541 tctgttacct gattatatct ccgagcaatg accccgtcta gataatcaga caaacctgag
     6601 acaacaatga ccgctcctgc tatataaaat gactgtctca gcacaaaata aagataaatc
     6661 ggcagcaaaa aaatacggaa tatcgataaa agattcggaa tcgtttgtat ttctttacgc
     6721 cagtctgtgc ttaatttcat tttatcgccc tacctcctca gcactctttt ctttttattt
     6781 tagcttacac ggggaataaa accaatgatt tcactgtttt tatttttctt tacagacact
     6841 gatattatga gctaatttga gataatcact tgctgttttc atcgtttact cttctttctt
     6901 cctgtcgttt gcgcatttct tttactagta ctttatgagc tttttttgat cctcgctcta
     6961 ggtcacgctg ccgtttacgt gttttataat cgataaacag actgtctaaa tcgtaacctg
     7021 tcgggtacag atcttctgct gaacgcacca gacgaacacg ctgacgaggc acgtggatta
     7081 tttttttgtc taaatataca tcaatcgtat cttcacctat atcttcataa actaacgctt
     7141 ctttttggta ttcattgaca aatatccgat caccttttgc aaagagctga cgttcttttt
     7201 tattctcctt cctatttgta actgctgtct taggaagaaa ttcttttttc tgtgtccgat
     7261 aggctttttc cgccaagtaa cgctctgctt gatggatcaa tttcatcgac atatccattt
     7321 tatgagcaat ccacaaagct tggctttctc ctgtttcttc caacccgcaa ctgatattta
     7381 ggacgaagtg cttcccgatc gaaagccata gcagccggca caaaatcttc atgatctccg
     7441 gcgaatttct tgatttctcc ataatgggtc gtggcaatga tcaatgcccc ttgctcatac
     7501 atagattcca tgattgcgat cgctagtgca gccccttcat ttggctctgt tccactgcca
     7561 atctcatcta aaagtaccaa cgtattcctt tttatctttt tcaaaatagc agcgatgttt
     7621 tgcatatgac cagaaaatgt actcaacgca ttttctaaat tttggtggtc accgacatcg
     7681 acgaaaatct catcaaagac agcaagctct gtcccttctt ttgccggaac ttgcataccg
     7741 aacatcgcca tcaaagtcaa taaaccgact gtcttcaaca cgactgtttt gcctccggca
     7801 tttgctccgg tgataatcaa gcctcgatag tctttaccga tttcaacatc caatggtacc
     7861 gcatgctcag gtaaaaatgg atgtctgcct tgcttgataa tgatatgttc cgatttattg
     7921 attctaggtg tgcttccatt gatttctcgg ctaaattttg cccgagcaaa gatgatatct
     7981 aacaccgtga tcgtttcgat caataaatcg atcacttctt cgttttctgc gatagcacca
     8041 gtcaatgccg ccaggacttg atattcttca ctgatttctt cagcttttaa caactgatag
     8101 tgttcattca atttagaaat cacagttggt tcaataaaaa ccgtcgttct tttatttgat
     8161 tcatcgataa tcgttccatc tattttattt ttatagctgg ctttgatagg aatcgtatag
     8221 tattcgcctt tttgaacaat cattgcttct tggatcattt ctttgttttt cggatgacgc
     8281 aagaatttca gtaattttga ctggatttct ttttcgatta gttgtaattg tttgcgtact
     8341 ttacgcaaat tacgtgacgc gtcgtcactg actttattat tttttatttt ctggtcaatc
     8401 aactcttcta catttatcaa atcaggaaga tgtttgctat aagcaaacaa caagggtgtc
     8461 tggtattgat tcttatcaaa aaactttgtg atcatccgac tgcttctaag aaaatccgca
     8521 tattcgatca aatcggttgg ctgcagaatc aacccttttt tgacttgttc tgtcagtgca
     8581 tcgatcctcg gcaaccccat aaatggaaca tgctgactgc tttctaagat caagcgtgct
     8641 tctcttgttt cttcttgacg atccaagaca gtttgtaggt ttgtcgagac cgtcatatcg
     8701 ctgattcgtt ttttgctata gtttccaatt gctcttgctt ggatttcctt tttgatccga
     8761 tcaaattgta attgactaat catttcgttg tccatattct ctccttttct tgaagagata
     8821 cctgtcccaa aataaataag ggcctagaga taaataaaag gatagaacac agcttaaaaa
     8881 agtcatgtcc tatccttatt agaatacaaa gatattgttt tcctgacagg taccgtttca
     8941 taagcacacc aaataaactt tgtttattgg ctattcgact tactgattag ataccagcta
     9001 cactcacaaa acatacccca ttcttctagg tcttatttat tttccactta actatagcaa
     9061 gacacatttt ggttgtcaaa ctttagtatg ttttatagat ttacaccatt aatgaataga
     9121 cacctaccgc gataattgct ccgatcattg gacctgcgat tggtacccat gaatatgccc
     9181 aatctgaagt tcctttatta gctattggca ataccgcatg tgctaatcga ggaccaaagt
     9241 cccgagcagg gttgattgca tatcctgttg gccctcctaa tgaaagaccg atggctaaga
     9301 tcaatccacc tacagcgaat acgttcgttc caggtgcaaa ttcgttttgt ccaaaagcta
     9361 gcaacccaag aactaataca aatgtaccaa ttaattctgt gatcacattt gctggataat
     9421 tccgtatagc aggacctgtt gcaaacgttc ctaaaatcgc gctttcatct ttcgttgcat
     9481 tccaatgtgg caaataagaa agccaaacaa gaatggcccc tgcaaaagct cctaaaactt
     9541 gcgcaatgat gaaaggaacg acaagattcc aactaattgc ccctgtcatc gccatagcta
     9601 gagagactgc aggattcaaa tgagctgggc ccatgaatcc agaaatatag acagccattg
     9661 ttacagcgag tccccaacca aatgcgatga caatccagcc agaagcctgt gctttacttt
     9721 tgtttaaatt cacagccgca cacacaccat ctccaagcaa aactaggatc agtgtcccaa
     9781 taaactcccc catgatttga gtcatatcac tgttcattta tttctccccc tttagttccc
     9841 gcagatctga ttctgcaatc aatgcttcta aacgtttagt ctgttgcgcc ttctcctctt
     9901 ctgtccattc aaaatatcca gcaattgcat cgatcacagg ttgcttgatc tcatcaagct
     9961 gatctcgttc aaacaacaga tgatttgtac gacgaatgag ataatcgcct ggggccaaaa
    10021 ccatttcttc ttctaaacca taacgtaacc gagctgactc agcgagactc aaaccaggat
    10081 aaggtgccat ttcttttgct aattcaaaga tacgtcgggc attagtcccg taaaaatcag
    10141 cgatataagt agcatcttct tcctctaaac cggctgctac tccttctttt gctaattctg
    10201 tcactgtttc ttctaattta gttggatcga agtttccacc tgaaatctga tattttttag
    10261 agtcgatttc tttcgtctct attccgtatt cttcttttaa taattggcgt atcaatcgca
    10321 aagctccttc tgccatctta cggtaatcgg tgattttccc accagataaa gtcagcaaac
    10381 catctggttc tctttctaaa gaactacctc tggaaatcgt agaaggtgct ttttcatcac
    10441 ggctgttttc caaatggttc aacacgtctt ctacttcagc acgagaaact ttgttttcct
    10501 tatattcact tactgtatcg acaactttat tgaagctctt gtctgaaatc gatccattat
    10561 ctccaccatt gtaatcagaa ccagaattac caatcaacag tggacgaagt cctgcccagc
    10621 ttgcttcgat atcattcaat gtgatatttg cttctggata gcgatgattg atcacatcca
    10681 atagataatc tacatcttct tgtgtgactt tcggatccgt aaaatctcct tggtaatccg
    10741 tatctgtcgt accaaagtaa gtcttgtttt ctcttggaat agcaaaaacc atccgcccat
    10801 cttgctttcc tgtatcgaag tatgtgggtt gcggtacagg cagttttttc gcatctacaa
    10861 ctaaatggat ccctttagtt ggacgcattt taggagagac tggacgcgta aaattcaagt
    10921 ttcgtacttt atctacccaa ggaccactcg tattgatcac taattttgct ttgatttcga
    10981 tcacttcatc tgtcagcagg tcacgagctt tgacgccaac gatttgatcg ccatcatata
    11041 aaaagccaac cgctttcatt ttacttacta gataagcccc atcttcggca gcctttttga
    11101 tattatcaat cactaaacgg gcatcattgt tgcggaaatc tagatagaca cccgcgcctt
    11161 ttagcccttc ttttttcaaa aacggttctc tttccaatac ttcttctggt gtcagcgtat
    11221 aattctcata tttggttcct gtcacatttg ccagtttgtc ataaaggtcc attgctactt
    11281 tgaccgagaa catattgaag gttgttgctc cttcgccctc atagattggc aaaagcattg
    11341 gatctggttt tgggatgtgt ggggcaatac tttgtacgac tgcacgttca ccaactgtgt
    11401 cagctactac ttccacatca aatgtcttca gataacgaat accgccatga acaagtttgg
    11461 tcgagcgaga ggaagtccct tctgcaaaat cctgcatttc gatcaatcct gttttgattc
    11521 ctgatgctgc tgactggact gccacaccgg caccagtgat tccgccaccg atgatcaaca
    11581 gatccaattc ttcttgctgc attttttgaa tgctatcttg tcttgtcttg tttgaaaaca
    11641 tatggatttc ctccttattc aaatgcttgg gtagctttta ctgctttttt ccaaccgttg
    11701 taaagttttt ctcggcgctc ttcctccatt tgaacttcaa atagcttgcc agcttcataa
    11761 aattcgcgga tctcgtctgt atctttccaa taaccgacag ctaatcctgc taaaaatgct
    11821 gcccctagcg ccgttgtttc caagttttct gctcgttgga tcggtacatt caagatatct
    11881 gtttggaatt gcatcagata ttcattgttt gctgcaccgc catctacttt caatacagga
    11941 atcttgattc cagtgtcatc ttgcatcgta tcgatgatat ctctcacttg ataagcaatc
    12001 gactgtaaag tagctttaat gatatcttct tttgtcgtac cacgtgtcaa gccaaacata
    12061 gcccctcgtg ctgcctgatc ccaataagga gcacctagcc caacgaatgc aggaacaaca
    12121 taaacctcat cttcgcttgt agagcgtttc gctgcgtctt ctgaatcgct agctttttgc
    12181 aacatttgaa gtccatctct cagccattga atcgaagaac cagccacaaa aatacttcct
    12241 tctaaagcat agtaaacctt tccatttatg ccgtaaccaa ttgtcgtcaa taaattattt
    12301 ttggataatt gcggtttttc accggtattc atgacaataa atgaacctgt accataagta
    12361 ttcttgacca tgcctggttc gaatgccatt tgtccgaata atgcggcttg ctggtctcct
    12421 gccatccctg aaattggtgc ttcgcttcca taaaaatgat aaccttgcgt ataaccataa
    12481 acttctgagt tagaagtgac ttttggtaac atcactcgag gaatatttag taaatctaaa
    12541 atctcctgat cccaatctag ctgatggata ttgaacaaca tcgtccgact ggcattcgag
    12601 taatcagtaa catgtgcttg tccatcggtc agtttccata ctaaccaaga atcgatcgtt
    12661 ccaaacatca attcgccttt ttcagctcgt tcttgcgcgc cttctacatg atcaagaatc
    12721 cagcgtatct tcgttgctga gaaataggca tcgatcacta accctgtttt ttcgtggaaa
    12781 aattcttgat agccttcatt ttttaagtta tctgcaattc cagctgactg tctggattgc
    12841 caaacaactg catgatagat tggacgtcct gtatcttttt cccatattac tgttgtttca
    12901 cgctggttcg ttatgccaat cccagcaatc tgatttggtt tgatccctga ttcgatgaac
    12961 gctccggcaa taactgactg tacagaattc cagatttcat ttgcatcatg ttccacccaa
    13021 ctttcttgcg ggaaatattg cgtgaactct ttttgagaac tgccgatgtg tcttcctttc
    13081 ttgtcaaaga taattgctcg tgagctagtt gtaccttggt caatcgccat gatatacgtt
    13141 gattctgtca ttgtgattcc ctccattata tgcaaacgct ttctttaact actcttatca
    13201 tactactgtt ttaaaaaaag aaactgtcaa aattttattc tttttttctt tttttgacag
    13261 ttttttatta tgaagctatt ttttaatcaa ttcttttctt ttttcagcgt ctctattctt
    13321 cttttcaatc gaataatgtc gtaaggtgac gcaaattctg acagataata ttcacactgt
    13381 tttttgtttc tctggcaaaa tgtgatcacc aaatcatatt ctttaccttc ttcataactt
    13441 ccaatctcaa taccgccgat tcccctcagt tctctgatca gatattgttg aaaagcaata
    13501 cgataaatcg gcagactttc taaatcgatt ccaatcacta ctggttttgc aatataaaaa
    13561 tcgatcatca aaagaacatt ggcatactgg atcatcaggt aactaagctc actatcttca
    13621 gctcttttcc tcggcaattg ctgcaccgct gtttcctgta atttcgccag taactgtgtt
    13681 aaggaatgcc cgagcatttt ctgctgctgt tccaaaagcc gatcacgctc atagacttcg
    13741 atctttccaa caaagaaaaa ccattcattg ttgatctggg aaatttggta gcctactgcc
    13801 ttttcttctt tgattttcaa acgacgagga cgataatgcc ataacatcgt ttcccgaatg
    13861 taagtatcta aaacagcggt tggtaacttt ttgctccgtg tcaaatcata ctgataaaat
    13921 atttcttcgt ctactatcga aaaagaaaca aaaaagctgt aaaacatcat tgtttcgtat
    13981 acattcagtt ctgctgctgt tcgactaaga tgcatcacga tgattggatc gattgcttga
    14041 taaagccggt ccgactgata gagtaatttc ttttctttca aagttgcgta cgtacttttt
    14101 tcattcaata gtcttttttt cgtaatcccg agccagcaag caatctgtgc ttctgctgat
    14161 gccgtaaacg ttgtttctaa tacacggtcc aatcctagaa tgaacggtct tttttctggt
    14221 gtattttgca aaaaaagcgg tcgctgatct tccgggatat attggaatag ttcataataa
    14281 aaatatcgta tctgtagctc ttctccgtat aattgaccgt ttttgatttg taatccaaat
    14341 tcctctaaga gctggttcaa ttctttgatc tttcgaaaaa cagaagactc actgatatta
    14401 aacgctgtcg ctaatcgaac gatggatact tgctgatgtt ctaataaata gacgagcatc
    14461 ttaaacttca agctttcctg caataaaaac aatagaattt tttccaaact tacatcttcg
    14521 tccaacacaa gcagcgcctg atactcattt cgctgtactt ctactttttt ttccatcatt
    14581 cggccaatca attcaatatc ttccaaatat tgatcaaacg aggcttttga cagttggaca
    14641 tgctctctca aaccattgat agaggctgtt cccccttgca gaacaaggtt tcgcaaaatc
    14701 aggatctgta actgctcttt tctttctaat aatgcttcaa tcttcatagg cagtattcgc
    14761 cacctttgac cacaccatat ctggcacacg gtattctggc tggaaaaata gctcatgttc
    14821 ttctaactgt tcaaacgtcc atccttcttc tactgcaatt gtcagttggt caagaaggtg
    14881 gaacatctct tttttgcttt ggagctgtgc tccaagaatc tttttttcag aatcaaatag
    14941 aaatttgagt ttaactttgc tttgatttat tgctgtaagc ttgtagtcta cgtatcctgt
    15001 ttcaattttt tggggataaa aaatgctctc ttcttctgtc aaacctacgc ttcctaagaa
    15061 atagccaaac cactgattgc cgactggacg ataggttccc tgatcctttg tctgtgagcc
    15121 actaattgtc ttgctgacat ttcgtgctgt acggacagcg ttatttacta gagaaacata
    15181 cattttttca tttgttggac gaaaagttac ttggatcgca tcgccaatcg cataaacgtc
    15241 tttttgagac gtttgcaagt agtcatctac ccagatcgtt ccatcatcat tcaatgttaa
    15301 ttgttcttcc cacatctgat tgtcgggacg agaatgatta gccagcaaaa caagatcata
    15361 gttctccttt cttttttccg tctctacaat agcggtttca ttttcggtca aagacacaac
    15421 ttgttcttct aaaaaaagtg aaagcccttg atttttcatt tcctttacta acggttctgt
    15481 catttcagga tctagataac gaaaaagaat tgactttcgg ctttcgtaaa gatggatttg
    15541 tttttttctt gatgccaacc cttcagctat ttcgattcct gcttgtcctg caccaataat
    15601 tgcaattttc tttgcattca tgatcttttg ctgaagagcc gttacgtttt gtatcttttt
    15661 aactgggtga ataagcgttg attctgcaga aatatttcga aacttttgat ttgaaccagt
    15721 cgctagaatc aaacgatcaa aatccaattt ttttcctcct gctaaaacga cttttttttc
    15781 tttcaatcct attactgtct tttctgtgta aacatggatt cgatagcggt cagtcagttc
    15841 ttctttcgtg atccaagaaa gttgtttttc actggtaacc tttcctttta atatcaaatt
    15901 gaacccgctg ggaatccatc ctaacttcga ttgcttttca atcaaatata tttcacttgt
    15961 tggcatcaat tggcgaagat aaatagccgc atgaatccct gcaaacgacc cgccgacaat
    16021 tacaatgttc atctgcattc ctctttctta taaggtgttc tactgctaac ttctcaatca
    16081 gaaaacacag tattcataac tcgctttcaa ccttatgata catagcgatg cagcagaatc
    16141 aaccctctcc atcataccgt gttctaggtc tgcttctcaa taatgtacta gtggacctaa
    16201 ttgaagagta tcatttctga agagatgttt caaaaaatgt actcttataa aagaaaaaaa
    16261 ctcgcctttt acgtacctct gtccaaatag gaaacagaaa agaacgaaaa gacgagtttt
    16321 agcgtgtgta atgtaatagt tagaatagac acatcattta tttttaattc tttaatgctt
    16381 caacaactgc ttcgcctaat tgatgagcag attgaggatt ttgccctgta agaagccgcc
    16441 catcaatttg aaccacttct gaaaaagctg ctccagactg atagtttgct cctttttctt
    16501 tcaaagcatc ttctgctaag aatggtactt cattcgttgt accatttgct tcctcttctt
    16561 cattactgaa cccagtcact gtacgtccag ctaaaataga ttttccagac tcatctgtaa
    16621 ctggcaacaa tcctacaaca ccgtgacaaa cagctgagac cagcccttca tttcgataga
    16681 tttgtgaagc gactttccca agatctattg cttctggaaa atcccacatt gttccgtggc
    16741 caccagcata ataaaccgct gcataatcat ttgcatccac atcatcagga cgtaaggtat
    16801 ttcccaaggc ccgatttctg aaatcttcat cttcataata ggcccaatcg atttcatcca
    16861 tattcaaaac actttgcggt tctaaaggta catatccgcc tttaggactg acaaaatctg
    16921 catcgatccc attttttact aatacatcat aaaaatgagt cagttcactt aaccacaaac
    16981 ctgtaggacg ctcgatcttt tcgtactttt ctacatttgt taatacgatc aatactttca
    17041 ttttcccatc ctctttctat aaaaattgcg ttattgataa cgtgctttca agaaattata
    17101 taatgggctt ggcaaataaa gctctttata tgcttcttca aaaggtttcg ttacaaattc
    17161 atcctcacgg ttttccaata atttctctgc ccagtcagga tcgacgatca tttgctgtcc
    17221 aattgctgca agttctgcat atttcaatac ctgctctaca tcttcgcgat tgcggatccc
    17281 accgacacca actaaaggga tctttccttg aatcgtgtta tggaccactt caagaatcga
    17341 tttatcgttg tatttatcag aacgagctat tcgatcatac atatttaatg aaacatgcag
    17401 ataatctagt tttgactcac gtaatcgttc aagaagccag atcgtgtctt caaaacggat
    17461 acctggtgtt tcatattctt ctggtgagaa acgataccct acgatgaatg gttttttagc
    17521 atattgttca accgctgaga aaaccgttgt taataattct tcaataaaac gataacgatt
    17581 ttccaagcta ccgccccatt gatcctctcg tctattcgaa tgcggagaga aaaattgttg
    17641 gatcaaatac gtatttgctc catgcaactc gattccatca aatcctgatt caatcgctct
    17701 acgtgtggct ttgccaaatg cttggattgt ttcaacgatt tcttcttcac tcattgctcg
    17761 tggtgtttcc gcatcaggac gttttgctgc aactgcactt gcagatacag gctgttctcc
    17821 agataaagtt gctcgacttg tcattcgtcc accgtggaaa atctgaataa tcgcttttgc
    17881 gccttctcct tggattgctt tcgctagctc acttaatcga ggaatcattt catcatgagc
    17941 gatgctgagt tcacctggcc agccttttcc gatatcttgg acatttgcag caccagtaat
    18001 aacagcacca agacctttag aacgttgttt ataataagtg atctcatctt cggtgatcac
    18061 tccattgaaa aagctgagtt gtgtcgtcat aggcgacagc atcaatcggt tcttcaattg
    18121 aactcctgaa ggtaaagtaa ttgattgatc aaattgtgac attgtcattc ctcccataat
    18181 tcgctctgat taacctatcg agaatcatct atcattaaac cactcgatta gttgtacatg
    18241 caactaattt gcttaaaaaa ttttattttt tttgtaactt atctgttgca tcattcattg
    18301 tctgggctaa ttctttactt gcttcatttc ccaaaactgc tttgtatgac tgatgaagtt
    18361 cttcccagct accatcgact tccttagcaa attggcgtcc ttgttctgta agataaacta
    18421 atgacaagcg accttcgcta actgttttga cgtatccttt atattctaat ttttcaacaa
    18481 aacgagcaat cgttgatggg gcggaataga gcatttgtgc aatatctttt tgtggtattc
    18541 catctttttc ttttaatatc aataaaataa aaccataagt tggtgccaga cctgtccttc
    18601 taaacgcttc atcagctaat ttattcaaca tgcgatctag ctttttcacg ctaaagaaaa
    18661 gacattcatc taacgctttt tcaattggct tttccatttc atcccctcca ttggcgtaca
    18721 tatcatagta tgatttttat tttttgtcaa aaaacacgga ataatgtccg cctcctctgt
    18781 ctagatttga aaaaaagaag tagccatctt caaagaattt cggcttgttt tcgacaaaac
    18841 tccttctgga acaccgtgaa tcagaggttg tgaaaaagtc gtttagtttt gagtatgaag
    18901 aaaaattttg tgaaaatggc tttccacatt tttgcataaa tttggcttaa taccgaaaaa
    18961 ctggcttttg aacaccgttt atatcagagg ttgtgaaaaa gccgtcctgc atcaagtaat
    19021 aagaacagcc aaacaaaaat agttcctcat attttgtcgt tttttgagct aatgccgatg
    19081 gtcagctgat tgtacaccgt ttattgagag gtcgtgagcc tgccgttgaa ctccgagtca
    19141 caaggagcaa tttcaaaaaa cagcttctca tgttttatga ctatggtgac ttgtgatcga
    19201 ggagttggca ggagaatact gtttattcgt aaagaagggg ctgtgacaag acttttgtca
    19261 cagtccctta tttcctttta ttataggatt tcgccatttg attggatgac ttgtctgtac
    19321 caatcaaatg attttttctt taaccgtttc tttgtgccat tcccttcgtc atcttgatca
    19381 acatagatat atccataacg ttttgacatt tgtgaagtag aagcgctgac gatatcaatg
    19441 cagccccacg acgtataacc aaataaatct actccatctt tgatagcttc cttcatctgc
    19501 tcaatatgct gcttcaaata gtcaatgcgg taatcgtctt gaacggttcc atcttcttct
    19561 actttgtcta tagctccgag tccgttttca gctatgaaca gtggtttttg atacctatca
    19621 tataattgat tcaaagcaat tctcaaaccc ttaggatcaa tctgccagcc ccattcactt
    19681 gatggaagat acggattttt aattcctcca gttattaagt ttccttggac cccttcccaa
    19741 ttttctggat gagcactgac tgcactagac atgtagtagc taaaagaaac aaaatcaaca
    19801 gggtaggctg ctaagatttc ttcatcacct acttctattt ttaagttgac acctttttct
    19861 tcaaacattc gttgcgtata cgctggataa tatcctcttg cttgaacatc actaaagaac
    19921 aaagaagttc tttgctgctc aattgttttt tgaatatcat ccggatcaca agaatacgga
    19981 acaagaagct gatagctgat catacaaccg acttgtgaac ctttgataat ttcatgacaa
    20041 gcttttgtag ctaaagcact tgctataaac tgatggtgtg caccttggta tttcgcttgt
    20101 tccaaatggg gatggttttc ttcaattact cctacgctaa caaaagggtg atgttttaca
    20161 cagttgatct cattgaaagt catccaatat tttactttat ttttatagcg tttgaatact
    20221 gtttcagcga aacgaacgta aaactgaata agctttcgat taggccatcc accatattct
    20281 gtaaccaaat ataacggcat ttcataatgg gaaagagaaa cgactggctc aatcccatat
    20341 tttagacatt cgtcaaacac ttgatcataa aaaaataatc ccgcttcatt gggtgtttct
    20401 tcatcaccat gaggaaaaat tcttgtccat gcgatagaca tccgatagca tttaaaaccc
    20461 atttcagcaa ataaacggat gtcttcttta aagtgatgat agaaatcatt tccgtgtctt
    20521 ttaggataat aatacttgct gttgggatcc tgcgccgctt ttacttgctg atgcgtcatc
    20581 atatgccatt gatctgtcca tttttcttta ggtaaatcgc tgtcgaatgt atacacatca
    20641 gacacagata atcctttttc atctacatcc catgcacctt cacattgatt ggccgctgtc
    20701 gctccgcccc ataaaaatcc ttctggaaat tttttcatat cgatccccct aaacttgcta
    20761 gttttcttgt ttgattattt ttcttatgac tagtaatgat atttttgaat ctactactat
    20821 ttgtaacaat aaccattgtg gtcggatcat atccctcttt gattaattct tgcaaatcta
    20881 cttccatcaa taaatcgcct ttacgtactt tttttccatc agatactgca ctttgaaagt
    20941 atttgccatt taaattcact gtatcaatac cgatatggat cagtatctct gctccttctt
    21001 cactgactac cccaattgca tgtttcgttg gaaaaactgt tgaaacttcc ccgtttacag
    21061 aactgtagat ttttccgtcc gttggaatga tcgcaaaccc ttctcctaaa gatccactag
    21121 caaatgttgg atcgtttact tcagataaat caattatttc acctgctgca ggagtactta
    21181 acactatatc ctttgaggat tcttcttcga tttcatcctt gtagccgaaa agaaaagtta
    21241 gcatacagga actgatcatc gctaagaaat aactagcaac tagccaaaca aatccttcac
    21301 caatataaat cgggaaagac aaaatacttc ttgtagcaac tgccaaagca ccggctcctg
    21361 cggcaccagc tactccacca aatatagctc cacatataca agcaatgtaa aatggtcgtt
    21421 tatatttaag agtcacacca taaacagcag gttcagtgat tccaaataat gctgtgataa
    21481 atgcagataa agataaactt tttactttct tatttttcgt tttcaaaaat accccaagta
    21541 ctgctcctgc ttgcgcagct acggcagtca tacatgctgg acctagcgta tcacgaccgt
    21601 agaaagaaag attgttggtc attactggta caagtcccca atgtaaccca aaaatcacta
    21661 acacttgcca aaatccgccg ataacagcac cagataaaac agggctaaag ttataaacag
    21721 ctaaatatcc agctgcaatc agctgacttg cataatctgc aattggacct acaactaata
    21781 aaataattgg gaccatcacg atgatacaaa tgaatggtgt caataaattt cgaatacttt
    21841 cgtgcaagct tttgcgaatc ttaggctcaa tataagaaag aacccataca gctaaaattg
    21901 ctggaataac cgtacttgta taccttgcta gaatcacggg catccctaga aatcgcaagg
    21961 ttatgctatc actatatgca ctgacaatcg taggataaac gagagctgct gcaataacca
    22021 tagcgatgaa acgatctgta ttgaactttt ttgcagcagt atatgccaaa aatataggca
    22081 agaaatagaa aaatgcatca gctgctgcat tcaaaatgat ataagttcct gattcagtag
    22141 ttaataaatt caaactcgta cataaggcga gtactccttt gatcaaacca cttcctgcca
    22201 agacgcctaa agtcggggca aaaacagcgg cgatcatgtc tataaaagag tttaaaatgc
    22261 cttttttctc agttcctctc tttccactag atgcatcatc ttctagtttc atttgtttgg
    22321 ataatacgtc atacacttca tttactgcat ttcctactac gacttgaaac attcctccag
    22381 cttcgactac agtgatcaca ccttctagtt gctctacctt ctccttatct gccttcttat
    22441 tatcttttag ggtaaagcgt aaccttgttg cacaatggta aacattgctg acattttctt
    22501 cgcctccgac aaatcggaca atatctttcg ctaattgtga atagtccatt tttacttccc
    22561 ccttgttaat taatgccgta gcaatccttc ttacaagata agtatagttc ttagaaaacg
    22621 gtttctccat tcaattagta ccccttctaa tagcgaacga aactgtacaa tagtgtgtat
    22681 ggtttactta gccgagcaaa tcactcactg cttgaaagaa aacaagtata tcgtttctct
    22741 tatagtcaaa ggagacaaaa aaaacacgct tgatcttcct ttgctgatgt ataagtggta
    22801 tctcaagctg atactctgtg atttttgttt ctacttggct tttgataaga cagcacaaca
    22861 aagtctcggc atttaacgga agcataacta ggtcagaact aggagagatt agttgctgaa
    22921 tggaatctat ctttttatgg atttttattt gattttcttc tttgtagtaa tgatcaagca
    22981 gttttgtttt ctttttttct tcaactatgg tcagcttttc acgcaaaatc tgataaatct
    23041 cccaagattc aaactcatct agaacactag gtagataaat caaatcatcc cactcaaaaa
    23101 taagctcttg ctcagtagtc aaaattgcat catattccgt tattttgttt gattgatttt
    23161 tgaaaagata cactggttct atccggcatt ctttgatcca catgttcata ctttgttgaa
    23221 tctgtttttt tagtgtattc aaaatactaa ttggctggtt actaacaatc agtatctccc
    23281 cttgttttcg aaaaggattc aacgcttcgg caagatacaa aacaaaataa gcgctttcag
    23341 ctaaattggg acgtaattga aaatgtattg ggaagaaacg ccgaattaaa taaatttcta
    23401 aaggataacg ggagatgatc ttttgtacaa aatgattcat ggtattatgt ccggcatcca
    23461 tcctcgtttt catttgtttg atatgggtta gcaaaaattc tgtttcatgg aacaataacg
    23521 aaacaggaag atctaaaaaa cgaataatcg tttgctcaaa tgctcttaga ttcttctcta
    23581 tttctttgtt tttttcatca ttaatcatca aataatttaa ctctaatagt cgattttcaa
    23641 ttgcttgttt ttcttgattg gaaaaagaat agggtagttc tgatgataac ttttttgtga
    23701 gggtctctac catcggaata aataatgtat acctgtttgt tttttctaaa agttcttctc
    23761 ttcgaagaat ggtcaaaacg agataccgag aaaatcgtat gaagtcctct cctgaaacaa
    23821 tataattata tgatataaga acctctttta atgtcttttg gacagtcggc atgatattgg
    23881 aaaaaattgt actgatttgt tgaggaaacc ttgcgtgttg gatcaccgtt tctgttccaa
    23941 caagagataa gaaaattctt tttgaatttt cttcagcaat gatttttagt ccttttgtcg
    24001 acgaaacttc cagttctaat ttaggattgc gctcgatcca acgcaaaatc gtttttattt
    24061 gtaatgatac ggctgttttt gaaagaaaga aaatgtctgc tatcttttgc atactgacat
    24121 accctttttg aaacaataag tacgtcacaa tagttgaagc tcggaaatcc atgctggaat
    24181 agattttatg tttcgtcacc tcttgtatga tttgctgttt agtttcagat tcaattgatt
    24241 ctaaaaaata acctgaacgt ttatttgact ggataatgat tttcttctcg tagatagcaa
    24301 gcatattttt gatttcagtt tggatcgttt tcttgttggt acctaaagct tcagctaatt
    24361 cttcgcttgt tatccaatga ttcttatttc ttaatactac aagaatttgg agttgtcgtt
    24421 tatttaagtc catttgctta cacctcctct ttgttataaa catacaggaa aaacctctca
    24481 aatgacaata gtcacttgca gaggtttaaa aatcatttga agaaagatct atattcaagg
    24541 tttttaccat tagactggat aatttgttta taatattcaa aactatcttt tttcaatctc
    24601 ctaaagcttc cgctcccatc attttgtcta tccacataaa taaagccata tcgcttcctc
    24661 atttcccctg ttcctgccga tacgatatcg attggtcccc accataagta accaatgatt
    24721 tggcaaccat cttttattgc ctcagcaatt tcctgaagat gtatttttag atactcacaa
    24781 cgttttggat cgtggattcg attcaattca tctggtgctt catctagtcc aatgccattt
    24841 tcaactatga ataatggtaa ttcataacgg tctgtcaatt cattcatgac ataacgaaac
    24901 cctagaggat caattggcca actccaaggt tcgggagatt tttccttcaa aaagggatta
    24961 tcgaaaccat ttgctccgcc agtatctacg cgcatttctg cttctttttt atagacagcg
    25021 ctacgataat aactaaatgc aatataatct gccgtgttag cttgcaagca ctgttcgtct
    25081 ccatctaaca ttactggtgc acagtcttct tccttccatg tcctgtaaat ataagctgga
    25141 taatgccctc ggcacatgac atcggagaaa aaccatgaat tgcgtttaaa ttgcagtgaa
    25201 ccaaatacat tctctggatc acagtcatat gcataagtcg ctaatgcaga aaagctacac
    25261 ataataccca tcttagcatt tggatcgatt ttctttaata attgagtagc acgggcgttc
    25321 gctagaaaca tatgatgaca ggcttgataa atcacttttt ttgttagtgt agcaatgggt
    25381 tcaagtttat ctctagcagg gaatgtaacc aatccagcag ctgcataagg catacgaaaa
    25441 gcattattga tctcattaaa ggtcatccaa tatttcactt tcccttggta gcgttcaaag
    25501 acggtctgcg cataaaattc aaaaaagtcg attaattttc gattttgcca ccccccatac
    25561 tctcctacca atgctaaggg ggtttcataa tgactaagag taattactgg ctcgatatca
    25621 tatcgattta atgtattgat tagtctgtca taaaatacga gaccagcctc atttggcttc
    25681 tgttcgtctc ctcttggaaa tattcgtgcc catgagatgc ttgttcgaaa agcttttaaa
    25741 cctaactcct tcagtaaacg aatatcctct tcaaatcgat gataaaaatc aatcgcttca
    25801 tgagaaagat aatggagtga ttcgtctaaa gcaatctccc aaatcttttt ttcttcattc
    25861 cactgaatac taggatgctg gttcatgatc ccgataagcg tatctgtcac attaggtaat
    25921 ttgccgtctt ccaaccatgc accttcacat tgattagcag ctactgctcc gccccataaa
    25981 aaattttctg gaaatatttc ctgatccata actatctctc ctcgctatca acaatttctt
    26041 acttgaagta tagctcgatt catcaaaaca agatattcta actatttcgg cagaagagaa
    26101 gaaaaaaatt gaatgctact cttcattttt aatatttata tataacaaat acataagacg
    26161 atccccgaaa gaaaacactc gttaaccatc taagcaaacg atttatttta cttccaacac
    26221 aaatgcttca taaggtcgta aatcaacttt gccgtctggt aactgtttca gaggataatt
    26281 tgacacaata acccttaaag cttcacgtgg aatagtgaac gtctgctttt cactggacat
    26341 attacaaata acgagccatt gctggtcttc atatatgcga gaataggcaa agacatgctc
    26401 aggcgtcttt tccactaatt catagtctcc ccagacgatg atgtcatgtt ttctacgcaa
    26461 atcgatcaat ttcttgtaca tataaaaaac agaatcttga tctgctaacg cttcttctgc
    26521 attgatttct ttataatttg gattgactcg aagccatgga attccagtag taaacccagc
    26581 atgttcacgg gcgttccatt gcataggcgt cctagcattg tctcttcctt tcgccttgat
    26641 tgattccaag atttcctctt ttgtaaatcc agaagaaatc ctttcgttat acatattgat
    26701 tgtctcaata tcttttgctt ccgcgatatc atcgattggg caattcgtca tgcctaactc
    26761 ttctccttga tagatataag gggtgccttt catcatatgg agcaagatag caaacatctt
    26821 ggcactttct tttcgataca catcgtcatt tccccaacga gaaacaattc gcggaagatc
    26881 gtgattattc caaaataaac tattccatcc ttcatcacct aaagatgttt gccatttgga
    26941 taatactttt ttcagttcag agatactcaa gggtttcaaa tcccatttgt ctttgccttc
    27001 ttgctgatct aatcctatgt gttcgaattg gaagaccatg gataattcat ttcttttagg
    27061 attggaataa agtttagcga tttcggtagt tgctccccat gtttcgccaa cagtcatcag
    27121 atttttgtca ccaaaagttg cttgattcat ttcctgaaga tactcatgca gtttaggtcc
    27181 attacttgtg atttctttat ccggttcttt gccaatcata tcgatcacgt ccatacgaaa
    27241 gcccccaata cctttatcga tccagaaatt catcatttga taaatctctt ggcgaacctg
    27301 tttatttttc caattcaaat caggttgacg cttgctatac aagtgcagaa aatattgccc
    27361 cgttttttca tccaactgcc aagcagaacc actgaatatt gagcgcaatc tattaggctc
    27421 ttttccattg accggatcac gccaaatata atagtcccga taaggatttt ctttattttt
    27481 acgggcctca atgaaccacg gatgttcatc agaagtatga ttcaccacca gatccatgat
    27541 gatacggata tttcttttct ttgcttcttc aatcagttct tccatttctt ccatcgtacc
    27601 gaattctgcc atgattgctt gataattact gatatcatac ccattgtcat cattcggaga
    27661 ctcgtatact ggagaaagcc aaattgcatc aatacctaaa tacgccagat aatccaacct
    27721 ggaaataatc ccccgcaaat ctccaatacc atcttgattt gaatcttgaa aacttcttgg
    27781 atagatttga taaacaactg aatggtgcca ccatttttgt tccatgattt tcctcctata
    27841 aatggatgtt tctatttttt gcttaatcat ttattttagt ctctctcact aaaaattgta
    27901 acgctttcaa atagctattg caccttgtta tcggtaacag aataaaacaa aaaaacagct
    27961 gaggaaattc cctcaactgt tttttgaaag gattgattta tgcagggaag aatgctgatt
    28021 tcacaacttc tttataaacg gtgttcactc agctgtccct tggtattagc tcaaaaaacg
    28081 gcaaaatata agaaactatt ttttcgtttt tttctctaat actcaggcca gaacgctgat
    28141 ttcacaacct ctttatttca cattcaatat ccacatgaat cccacaaata caaagaacag
    28201 accatacatg attggagata cttcttttcc tcttttagct gctaacatgg taattggata
    28261 gaagataaaa cctaatgcaa tcccatctga aatactataa gtcaaaggca tgccgatcaa
    28321 gatcaagaat gaagggatcg caatctctaa gtttccccat tcgatttgtc gcaaagattg
    28381 tgccatcaat actcctacca taatcaatgc tggtgcagtt acttgtgaag taacaactga
    28441 tagtaatgga gaaaagaaca agcctaacaa gaagaaaatc cctgtagtaa tagctgtgat
    28501 acctgaacgt cctccaacgg cgataccagc tgatgattct acaaatgcac caacaggaga
    28561 agtaccaaat aaggaacccg caagcatagc tgttgaatca gaagctaaag ctttccctac
    28621 acgaggcatc ttgttgtctt tcatgaaacc tgcttggttt gctaatccaa ctaaagttcc
    28681 tgctgtatca aagaaagtca caagcaagaa tgtcagcact acgacccaca tttgaagaga
    28741 attgatgtcc cctacatgtt ttaatgcaac taaaaatgtt ggtttcaaac ttggtgctgc
    28801 ggcaacaatt tcagaaggca ttggaatcaa acctgtgacc agacctaaaa tcgttgtagc
    28861 tgccatcccg atgaagatgc cacctggtac acgccgaaca agcaagatag cagttacgac
    28921 taatccaaaa atcgtcaacc aagtagaacc aacgttgagt gatcctaatc ctactaatgt
    28981 agattcattc gaaacgatga tcccaccttc gctcagacca aggaaagcga taaatagacc
    29041 gataccgcca gaaatcgcat atttcaaatc tgctgggatc gcatcaatga tcatttcccg
    29101 cagcttgaag attgtgatca aaataaaaat caaagaagca acgaatacgc cggctaatgc
    29161 tgtttcccat ggaatcccca ttcctaaaca aacagaataa gcaaaaaaag cattgatccc
    29221 aagagcaggt gccgttccaa ttggataacg ggctaatacc cccattaaaa cacagccgat
    29281 cgcactggct aatgcagtcg cagtaaacac tgctccttca tccattccag aagcacctaa
    29341 tacagttgga tttacaaata agatataagc cattgagata aatgttgtaa atcctgccag
    29401 tacctcacgt tttaccgtgg tgttcaattt ttctatttcg aagtaggaaa gtattttttc
    29461 cttcatcttc catcccccta aatccgaaca ttataaataa agtccgttaa aatttcgatt
    29521 tattaataaa acaacgtgtt ttatcatata ctaaaacaaa acataagtaa acaaaaaatt
    29581 cggattttaa gcaaaatccg aacgttccat ctgattaaat ccctttatct caaagttcat
    29641 gaacattcac tttggattac aagaatttct ttattgtgtt aataaagttt cttcattcta
    29701 gtctcaaaaa tcaatagtgt gatttgattt taccatttcg tttctgttct tctttgatga
    29761 attcttttgt atcgacagga ttcagattat ttagtccgcc aaaacctgta tcgaccattt
    29821 ttccccagcc ttttgtcttc ttttttcctg atttgagatt ctttgctcga agcttctcac
    29881 tatgttttgc tcgccgatac ttcaaataac cttctaatct tcccattaga tccactgttt
    29941 ttcctccttt ttattcaatc agacgactca gcttttccta taacaaaata attgccttca
    30001 gggtcagaaa aattgaaagt caagatgcca ttgttatctg tgatttctcc tggttcttcc
    30061 atgttctcgt agatagtttc aaattcatcc gtaaaaaata ctaaagaagg cggtggtcct
    30121 aatacctctg gtgaaaactt ctgcacaaat acttttggga atacgcctaa ttctaattct
    30181 tttgagatgc tgagtacaat actcttaaat tcttctggca attcgattgt ttcagaaatt
    30241 tctgcaccaa ttttttctac aaagaatcgg caaatcatct ctacgtcatt gacataaagc
    30301 atcacacgtg ttttttgtat catcatttga cccttctttc tatttgattt tctttcctgt
    30361 ctataaactg attccattat atcacgaaag cgcaaacacc ttgtgtttga tactattcaa
    30421 gaaaaataaa gcaatagttt ctaggattgc tttcctccac aaactttttt tacaaaaaaa
    30481 aaacggcaat gaatgccgct cttcttattt cgattctttt ataattctgc ttctgattca
    30541 tctttggtta gtgtgtgtct aacactagtt acgccaaatg tttgatcgat tttttccttc
    30601 aacgcttcga ttgcctgtgc gccacgtgcc gcttgttcag cattgatgga ttcaatgacc
    30661 agcaccgctg cttgtgtttc ttccgtcagc atcgttcttt gggcttcacg ccagttgaga
    30721 cagcgacaga tcgctccttg ttcatcatag tagatgattt cttccggaag tgccggagca
    30781 tcggaatctg cacctaaagg caggaatgat tcgccacctt gggcttttcc taaatgcaga
    30841 tttcctgcga tcttttcgat atcttctccg ccgcaaggta cgccaaaaga caaggaaata
    30901 ctattataga tatctacaag cggattgatt gggaaaaatt cccgttcttg atttgcacgt
    30961 ttcagtaaag cttcaataga agaacgtgca ccttttttag tcttgaatgt ggtaaatgcc
    31021 tgacgccact gagcaatgac ttcgttttgg ctgaatgttt cttctgtaag gtaatttctt
    31081 gcttcttttg ctgcctcatt caataattga tgatacgttt cttcactttc ttttgttacg
    31141 tggttatcga taccatttac ggataagatt tcgatggttg cttcaggaaa tacttcccag
    31201 aaattggtat ccactattac ttttttcatc tgttcacacc tcttgattag aataattgct
    31261 tgatgaatag atagtctgtt tggggatcat ctcccatata aaaaacatgt tcgctgtatt
    31321 tgataaatcc ttttttacga taaaaagcga gcgcaacatc gttgtgttcc caaacaccga
    31381 gccaaatggc tgttttttgc ttttcttttg ctttttcaat agctgtatct agtaaaaatg
    31441 taccgatacc ttgatgttta aatttttttc gtacatagat tcgctcgatt tctaaaaaat
    31501 gatcagccat ttcctcagat tgactcgttc ctgtatttag tttcaggtaa cccgcaggct
    31561 cttgatcgat actcactagg aaaaaatcgg tttctttatc ttttagctct tctgtaagtt
    31621 tttctagtgc ataagcttgt gtcaagtaat cagctaaatg ctccggtgaa ttatcttttc
    31681 caaatgtatc tgtaaatgtt tcgatactaa tttgttgtag taagtctaca tctgaaggcg
    31741 tcatttttcg aatttcgtat ttattcacgg cttcttcctt tcaatagatc cttttattgc
    31801 cttttttgac aaaattccaa tcttcgctaa cattcttgca tactttattt aaaagatatt
    31861 ccaattgttt tgcttcttcg tcagaaagac catttaatgc gactgcattg gagtagttgt
    31921 tttctcgaat gatgaaagga tagatttctg ctcctttttt agtcggatag agtttcttga
    31981 tttttttatt ttctttatct tccaaacgtt cgatcatccc attggattct aatttcttga
    32041 ttgctcgggc agtcgttgta cgatctactt tgatcatttc tgctagtttt tcttggatga
    32101 ttccaggatt ttcgcaaata cgtactaaat aaagatactg tcctcttgtt aaatcgactt
    32161 ctttgaattc gatattgcta atagaatcta gcgctcgggc aatcattccg atgtctcgaa
    32221 ggatttcagc catttcatcc cacctttatt ttgtttgtaa gactagttca cctctttttg
    32281 ttgcaaatgc aactattttc aataaaaaaa tcaagttctt ttttaataac tagtaaagta
    32341 tattcttttc ttcttttacg aaaaagctca atcacaatag atctggtttt tgttttctaa
    32401 aataagtaac tgtttcttcg ttgttcgctt ttctgatagc tttggcaact cttctatact
    32461 aaaaaatccc atgttggaag tttcattatt ctcaataaac ttggcctctt caccgtggat
    32521 cgcacaagca aaaacaagtt tataatactg aaaaagctgt ggaatatctt tttgtttatt
    32581 cgtatcaaaa actgctcgca actgagtagt ttctactgtt aatcctgttt cttcatagac
    32641 ttctttacgc acattttctt caggagacaa tcctatttca gcaaatccac caggaagtga
    32701 ccattctttc gtccgtttat cttcgattag taggaccttg ccatcttttt tgatataggc
    32761 acgaacatct acttttggtg ttggatgtcc ttctccaatc gttgtgagcg cttttatttc
    32821 ttcgaaagtt tcttttccta tttcactgat cagttccaat gaaatcgtcc ggatctcctc
    32881 aaaacgctct ttatcaaata catcttttcc ataatataga ccagcttgag aaagtgcgag
    32941 tagtcttttg tattgagcca acttcttgtt cattctcttc ccttcttttt tagcaaatgt
    33001 atgactatgt aatcaagtat agaagactgg tttaattacg gcaaataaat ataatgaaat
    33061 ctgcaataaa taaaagaatg ttaaaaaact cgagaacaaa agacattcga cttctctggg
    33121 gctgtgaata aatggcgttc aaaagcttgc atctgcggta ataagcccca ccaaacgaaa
    33181 aatatgacga gctattcttg tctggctgtt cttattaatt gaggcaggac agctttttca
    33241 caacctcccc actaaacggt gttctcctac caactcctcg gtcacaagtc acaatattca
    33301 tcaaacatga gaagctgttt ttttgaaatt gctccttgtg actcggagct gaacgacagg
    33361 ctcacgacct ctcggtaaac agcgttcaaa agccaatttt tcgatattaa gccatttctt
    33421 tgttacttat tgtcattgat ttctttttct aatttatcca atagtggagt gtaatccact
    33481 tgagtatagt ctgttacatc aaattcatat agtgttccta actggaattt gtcatatttt
    33541 cggtcggcga tgatcttttt gaatgcttct ttcgtaatat gattggtccc tttcgttcga
    33601 tcttccaata cgtcaccata gtgataatga tccatgatat agctcaaatg acgatctgat
    33661 tcacgatcac gtttgtaccg acgttgccat aacacttcaa agtctgctgt taggcgaatc
    33721 gtgatcacct gatatccgta tctatctgct aattcttgga gtcgtgcttt ttgcttatca
    33781 ctaaaaggat actcggacac aatcactttc tttcctgctt ccatatacaa atcaagtgct
    33841 ttgtaataga aaggataaac tttcgtcctt tcttgttccg cccgttcttc caaattgtta
    33901 aacccgatgg attctgcata gtattccttg atttcatcga gcgtgatggc atacatatct
    33961 ggaaatcgtt cccgtagttt attcataaga tatgtttttc ctgtaccagg actaccagcc
    34021 agaagaatga aatacttttt catctcattt atttcctaag ttaattggac tttccaagtt
    34081 cataaacagt ccaatcttat gcttgacaat ttcttttact gcttcatttg ctggaatcac
    34141 attatggcga agagacttgt tgatttctgt ttcactaaag gtttctttag ctactttcgt
    34201 ccagttgact aaaagctccg ttccaacatt gaatttgcgg atcccgttat tgatcaattc
    34261 aggataatct tcttctttca cacctgttcc tccatggata actaggggga catcaactac
    34321 tgcatcgatt tctttcagca agggaatgtt tacttctgtt tttgatttga attgtccatg
    34381 attcgtaccg atagcaatag ctaatgcatc cacgcctgta gcttctacaa atttcactgc
    34441 gtcttccggt cttgtatata ccttgtcatt ttcagcgaca tggattcctt cttctgtacc
    34501 gccgattgtt ccaagttctc cttctacgga aacaccgtgc gcatgggcat attctactac
    34561 tgcttttgtc ttcaggatat tttctttgaa aggcagatgg gacccatcat acatgacaga
    34621 agtatatccg ctgtcgattg cttctttgat atcatcaaag tctcgggcgt ggtctaagtg
    34681 caagactgca tcaaccattt cttcttctgc aatagctgtc attacattga ctaacacatg
    34741 catgccgata tatttagcgg tatcgacact tgtttgaata atgatcggcg aacccatttc
    34801 ttttgctgcg cctagcatag caggaagcat ttctaggtta tgcgtattaa aagctccaac
    34861 tgtaaaattc aactcttctg ctgtttttgt tacttctttc aatgttgtat acatctttta
    34921 ttgccacctt atctttttat ttgtttcctt tgatcgttac ttgttttgca atttgtcgca
    34981 tctcatccat tttgcccatg tagtaatcga tcccttgatc atcaccagca cgagcagcat
    35041 ctgcacgctt ctggttatag aggctcatca gttttttata cccatctccg atcgtctgtt
    35101 tattttccaa actcttttct aaatattgga tggcttgatc gttcacatac aattcagaaa
    35161 atttaagtcc aacttgttca taaagtttcg ctaatttatg cttatcttgg gtagtggaaa
    35221 tctcttgttt caattgtgta atctctgatt gaatcttttc ggtcgcttct ggcgataggg
    35281 ctttttcttc ttttgccaga acttttttcg gttctttttc ttttttctta aaaaatccaa
    35341 acatcatttt ccctactttc aattaaaatg ctttcaaaac tgggcttaag atccaggcat
    35401 ataagagtgc tgcggctact gtatctacat cggttgctgt agcattgttg aatcccatcg
    35461 aattaaagat cgttactagc aaagcgggca ataatgtaat aaagaaacca tgagcaatac
    35521 cgccaatgat cgctcctcgg cgtccgccaa ctgcgttgcc aaatatccct gctgtccctc
    35581 cggcaaagaa attggttagc ataccaggta aaatcatcgc taagccgaac atgggcaaag
    35641 taaacatggc aataatcgta ccaatcgttg tcgtaatgaa acctaaaatg acagcatttg
    35701 ggctataagg aaagaaaact ggacaatcta aggcagggat cgcatcagga actaatttca
    35761 tagctatccc tcgaaatgcc ggcacgattt cacctaacag tagacgtaca ccagctagca
    35821 atacatacac acccactacg aattggatgg cttgtaaaaa ggcaaacatg atatagtttt
    35881 gatctcctga taattcagaa gcgaatggtt cgcctgcaaa taaaacagtg atgatatata
    35941 gcgggaccat cacgaccatg acagacaaat aagtatcttg cagaaattcg aaatgtgtcg
    36001 gcaacttgat atcttcgatc gactttttat tttcgccttt ctcaccaaaa agcttagcaa
    36061 caccggcttc aaataagtac ccgatcgtac aaaaatgacc taaagcaata tcatttgatc
    36121 cagtgatttt acgaatgatc ggttgcgcta ctgccggcat agcaatggcg aaacaagctc
    36181 cgataaaacc accgaccaag atcaatacga tccctcgcag accggcaaaa tagccaaaga
    36241 ctgtcgtcat cgttgccatc cataaaattg cttgaccagt taaaaaaatg tacttccatt
    36301 ttgtgaaacg agccaaaata atattgaata caaaaatggc taagaacgtc agtgcaatat
    36361 ctctacctaa acccaaatca ttcattgctt gaccattgat agattcaata gaagggatga
    36421 tcccttgcat atgaaatcct tcagtaaaga ttttcccgaa ataagttaaa cttccaacga
    36481 tgatgctcga accggcactc aacacttgaa atcctaggag tgtctttaac gtgccagaga
    36541 tgacttgtcc tgctgatttc ttctgcagta gtagacccaa catagcaatc aatgcaatgg
    36601 tgatcgatgc ctgagtcaaa atattttcga tgataaaatt gataacgccc atgttttgat
    36661 gtgcacccca aaagttagac tagaaatcta attaaaaggg gtgctttttt tgcggaaata
    36721 tacatttgtt tttaagaaaa aagtagtttc agactattta aataacgaag gcggctacaa
    36781 atatcttgca cataaatatc aaataaatcg tacactggtt agacattggg taaggattta
    36841 taactatcat ggttgggaag gcttggttgg aggtggcaaa agctacacta caaaatttaa
    36901 acttgatgtt atagaatata tggaaacaaa tggtctttct atccaagaaa ctgctaaaaa
    36961 atttaatatc ggttcaaata gaactctaag taaatggata gagcaatatg aagaaggcgg
    37021 tgcttcttca cttgagagcc aaaaaagggg cagaaaaatt agtatgaatt ccaagctaaa
    37081 cattcctaaa aaacttaaag atgagtctct tgaagaagaa gttattcgtt taagagcag
//
LOCUS       DACDUU010000022        36567 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.022, whole genome shotgun sequence.
ACCESSION   DACDUU010000022 DACDUU010000000
VERSION     DACDUU010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.022"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            120..698
                     /locus_tag="IWL30_001123"
     CDS             120..698
                     /locus_tag="IWL30_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP9705905.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            698..1627
                     /locus_tag="IWL30_001124"
     CDS             698..1627
                     /locus_tag="IWL30_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9705906.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            1624..3360
                     /locus_tag="IWL30_001125"
     CDS             1624..3360
                     /locus_tag="IWL30_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9705907.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(3423..4259)
                     /gene="pheA"
                     /locus_tag="IWL30_001126"
     CDS             complement(3423..4259)
                     /gene="pheA"
                     /locus_tag="IWL30_001126"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAP9705908.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(4256..4765)
                     /locus_tag="IWL30_001127"
     CDS             complement(4256..4765)
                     /locus_tag="IWL30_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAP9705909.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(4758..6053)
                     /gene="aroA"
                     /locus_tag="IWL30_001128"
     CDS             complement(4758..6053)
                     /gene="aroA"
                     /locus_tag="IWL30_001128"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP9705910.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(6066..7157)
                     /locus_tag="IWL30_001129"
     CDS             complement(6066..7157)
                     /locus_tag="IWL30_001129"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAP9705911.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(7174..8340)
                     /gene="aroC"
                     /locus_tag="IWL30_001130"
     CDS             complement(7174..8340)
                     /gene="aroC"
                     /locus_tag="IWL30_001130"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAP9705912.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(8342..9406)
                     /gene="aroB"
                     /locus_tag="IWL30_001131"
     CDS             complement(8342..9406)
                     /gene="aroB"
                     /locus_tag="IWL30_001131"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAP9705913.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(9439..10461)
                     /gene="aroF"
                     /locus_tag="IWL30_001132"
     CDS             complement(9439..10461)
                     /gene="aroF"
                     /locus_tag="IWL30_001132"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAP9705914.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(11120..12094)
                     /locus_tag="IWL30_001133"
     CDS             complement(11120..12094)
                     /locus_tag="IWL30_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAP9705915.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(12075..13958)
                     /locus_tag="IWL30_001134"
     CDS             complement(12075..13958)
                     /locus_tag="IWL30_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAP9705916.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(13976..16201)
                     /locus_tag="IWL30_001135"
     CDS             complement(13976..16201)
                     /locus_tag="IWL30_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP9705917.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            16316..17350
                     /locus_tag="IWL30_001136"
     CDS             16316..17350
                     /locus_tag="IWL30_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9705918.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(17417..18430)
                     /locus_tag="IWL30_001137"
     CDS             complement(17417..18430)
                     /locus_tag="IWL30_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9705919.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(18508..18696)
                     /locus_tag="IWL30_001138"
     CDS             complement(18508..18696)
                     /locus_tag="IWL30_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAP9705920.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(19136..19264)
                     /locus_tag="IWL30_001139"
     CDS             complement(19136..19264)
                     /locus_tag="IWL30_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP9705921.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(19267..20640)
                     /gene="rlmD"
                     /locus_tag="IWL30_001140"
     CDS             complement(19267..20640)
                     /gene="rlmD"
                     /locus_tag="IWL30_001140"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP9705922.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(20751..21806)
                     /locus_tag="IWL30_001141"
     CDS             complement(20751..21806)
                     /locus_tag="IWL30_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP9705923.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(21816..23246)
                     /gene="gatB"
                     /locus_tag="IWL30_001142"
     CDS             complement(21816..23246)
                     /gene="gatB"
                     /locus_tag="IWL30_001142"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAP9705924.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(23243..24715)
                     /gene="gatA"
                     /locus_tag="IWL30_001143"
     CDS             complement(23243..24715)
                     /gene="gatA"
                     /locus_tag="IWL30_001143"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAP9705925.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(24715..25020)
                     /gene="gatC"
                     /locus_tag="IWL30_001144"
     CDS             complement(24715..25020)
                     /gene="gatC"
                     /locus_tag="IWL30_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAP9705926.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(25126..27162)
                     /gene="ligA"
                     /locus_tag="IWL30_001145"
     CDS             complement(25126..27162)
                     /gene="ligA"
                     /locus_tag="IWL30_001145"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAP9705927.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(27179..29413)
                     /gene="pcrA"
                     /locus_tag="IWL30_001146"
     CDS             complement(27179..29413)
                     /gene="pcrA"
                     /locus_tag="IWL30_001146"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAP9705928.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            29568..31127
                     /locus_tag="IWL30_001147"
     CDS             29568..31127
                     /locus_tag="IWL30_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAP9705929.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVVGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(31238..33169)
                     /locus_tag="IWL30_001148"
     CDS             complement(31238..33169)
                     /locus_tag="IWL30_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP9705930.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(33187..34101)
                     /gene="pfkB"
                     /locus_tag="IWL30_001149"
     CDS             complement(33187..34101)
                     /gene="pfkB"
                     /locus_tag="IWL30_001149"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP9705931.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(34098..34850)
                     /locus_tag="IWL30_001150"
     CDS             complement(34098..34850)
                     /locus_tag="IWL30_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9705932.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(35009..35218)
                     /locus_tag="IWL30_001151"
     CDS             complement(35009..35218)
                     /locus_tag="IWL30_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705933.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(35229..36557)
                     /locus_tag="IWL30_001152"
     CDS             complement(35229..36557)
                     /locus_tag="IWL30_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705934.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
BASE COUNT         9929 a         7909 c         6662 g        12067 t
ORIGIN      
        1 ttagcatcta gaccaaagat cgaaatggtg aggtttcttt aaaataaaaa tatccttgca
       61 ttttctccta gaaataagta tttttaaatt acaagactat tcttaaggcg ggtaaaaaaa
      121 tgccaatacg cgtaattctt atcgatgatg aacagacgat tttagatgga atgaaacagt
      181 tgatcaattg gtctgagcac gggtttcaat taatagggac tttttccagt ccgctgaaag
      241 cgcttgattt ttctttggta catgcaatcg atatcgtggt cacagatgta atgatgcctg
      301 aattaaatgg aatcgaatta tcgcggctat taaaacagca acaacctgaa acacagatcc
      361 ttatcctctc tagctatgat gaatttgata tggttaaaga ttcttttaaa gaaggcgtcg
      421 ctgattatct tttaaagcct cgattgagtc ctgatttttt tctaaattcc ttaactgccg
      481 tttcacaaaa aatcaaaaaa aagcccaaca aaagcccaat caccaatcgt tatgagcaga
      541 tctgcgagaa tctttcacag cagattgctg gacaaactcg tccattgctt gaccctcttc
      601 tttttgaaca caccttgttt ttctgttata taccgaaaaa cggccattca gttcaaaaca
      661 aggctctaaa caaaaacagt cgatcaccca ttcattgatg gatagcgacg attcgtttac
      721 tatttatcct gtttctacct tttcggaaga atccggctat gtaatcaata ctgattccaa
      781 agatacactt aggaagtttg ttcaaaagtt tcgtcaagaa gtttttccag gtgatttttt
      841 tgttgtttct gattcttttg caatccaaga tatcaatgaa atattccaag agatcagaaa
      901 ttgcaccaaa ggacaacgtt tctatcataa gggaaagaac cttgcttatc agcatgaact
      961 tcttccgtta ttaaccaacc acgagcagca agcaagtagt tatttaacaa aaatcgttca
     1021 taaagactac gctttagctg tcaaagaact tgagaatttc ttcacgacga tattggaaca
     1081 gcctattgat ccagcttatt tgaaacatga ggcaatcaat cgtttctatg cattactcaa
     1141 cgctttggct gacgagcatg ttgggaatga agaactttct ttgctcaaga tcgcccttcc
     1201 ttctttatta gccgatgctg agtatttcga agatttttct attcttgtca aagaaacact
     1261 tgagaaactc acccactttt ctgcaaatgc aaaaaaagac aatttcgata tcttgacttt
     1321 gatctacgag ttcgtccagg aaaattatga gaaagaaatc aatttacaac ttttatccga
     1381 aaaatatcat ttttcatata gctatctctc agccatcttt actgaaaaat ttggtatcaa
     1441 cttttcaaaa tatctaaaaa aagtccgtat cagtaaagcc aagctctttt tgacggaaac
     1501 acggcttact ttgacggaaa tctgtgagaa agtcggctat accgaactgg gttatttctc
     1561 cagagttttt aaagaagaga caggaatgac accttctcaa taccgtaaag gaacttttgt
     1621 aaaatgagaa aaaaaatcaa agagcatgaa ctctttgcca aacttctgtt cattttgttc
     1681 atcggtcttt ttctgcaagc catcgtcatt tctttgttta tttatcatcg atcaagggat
     1741 gcctatatcc agctgttcaa tcaatcgaat gatgtggtac taaaaaagat tcaatctgaa
     1801 tttgaaagtt tgaatgatac aatcgagaat acattagccg cttttgattc taatccagct
     1861 gtcaaaagct atttttccaa tgatccagcc caacatatgg aacagcttca gcaactaaga
     1921 acaatccaaa aaatgaatga ctctctttca aaaatccatc caatgatcga ttacgatgta
     1981 ttgatctttg gagaaaatgg aagaactttt gtcggaaacg acatgctcac tgccgtatct
     2041 gccgattctt tttttcaatc tgcgattgct cagagagtga atgaacgtgc tgctgataca
     2101 caaatgcttt ttgcacacca cggattgact cttcgcgata agaaagcacc cagcgtcttc
     2161 tttgtcaaaa aactgaaaaa tacattgaat cacgtttacg gatatgctgt tttatcgatc
     2221 tcatccaagc aactagcgaa tctttttcaa tctgtagtaa atcctgagat atcgaagatc
     2281 tccttcatga acgaagaaca gacgatcatc gcatcaaatg aagaaaatac aattgggagc
     2341 cgatcaatga ttttcaaaaa actaacagta ggtgaaacaa cgatttcaaa tggacagcag
     2401 ttgacccgtc ttcctctgta ccgccaggat tgtacattac tcagtcaagt cgatcttcat
     2461 tctttatcta accaaatggg cgtgattttt ccgattattt tgtataatat cgtgacactt
     2521 gcttttgctg gctcattggt atttatgtat ttggatcgac acacaaaatc gatctaccgc
     2581 ttgattgatt cgctaaaacg gctagaggaa gatggcttaa aagctactgt tcctgtacaa
     2641 ggaagccatg aagtccaatt gctagggaat acgatcaatc aattgctaag taatgctcag
     2701 catcattatg aacagacgat ccaagacgaa aagaagaaac gttcgcttga aatccaaact
     2761 atgcaagcac agatacagcc ccattttatt tataatacgc tgactagctt gaaatttttg
     2821 atttggcaga aaaaaacaga tgaagcagtt caaggaatcg acagtttcat tactttacta
     2881 agaagtacga ttgggaaaaa agaagaagtg attccagtca aggaagaaat caaaagcgta
     2941 caaagttaca tcgatctttt gagtatgcga tatggcgaaa gaatctcggc taaaataatg
     3001 attcctgatg aactgcagtc attatctatg ccgaatatga tccttcaacc aattatcgaa
     3061 aacgcctatc ttcatgcttt tcacaataaa acgcaaggat tcatcacggt gtacggcaaa
     3121 acaaaaaatc agcttctttt attcgaagtg attgataacg gcgatggctt cgatactgaa
     3181 aataccaaac aaaaaaatga tcatttttca ggaatcggca tcagccatgt agatgagcgg
     3241 atacgcctaa tgtatggcga tgcatatggc ttaacgatcc aatcagtgat tgggacagga
     3301 acgactgtaa cgatttgtct accgcttctt cctaaaaatc aatcagaata tcaaaaataa
     3361 agaatagcca agcgctgatt tttttatttt aagttaaata aatcaatcag tattcttgac
     3421 tttcatctac gcgtctcttc tactgtcagc actggataac agcccaatat ttgactttct
     3481 cctccgagca tcttgatttc ttggagcgca ttctctacta atgtcattgg acggtccaac
     3541 aacaaatcga tcacgaaaaa atattctccc aagctagttt tcaatggtcg ggattcaatc
     3601 ttacttaagt tgatttcccg ccagccaaaa gctgccagca ttttatgaag catccctggc
     3661 cgattagcag gtagtgtcaa gatgacactc atttttactg gagctgactg ctggctggtc
     3721 atctttcggt caccgacgat ccaaaaacgt gtctggttca actcattatc ttggatattc
     3781 tcagctaaga tctccaatcc aacatgctgt gctgtttcta atgaagcaat cgctgctgcg
     3841 tcttctttcg gatgttctgc tacatacatt gctgcagcag ttgttgattc tgttgctact
     3901 aaaggaacat taggataatg cgtttctaaa aactgttgtg attgtgctag tgcttgtgga
     3961 tgtgaaagga tttttgtaat ctttgttgct ggattgccta aaagctgctg cttgatagga
     4021 agaacgattt cacttttaac ttcgacttct ggatgtttgg aaagaagatc gattgtgtga
     4081 tgaacagctc cttccaatga attctctaca ggaacgacag ccagatcgac ttgtttgcga
     4141 aacaaagcgc gcaaacaaac agggatggaa gcatacgcag tcaattgttc tgtaggaaat
     4201 agctgacaag ccgcctgata agtgaatgaa ctttctggcc ctagatagct gacgttcata
     4261 tgcttttgat cctttctata atggtatcga tgatcttttc tggtgtttga ttggtcgtgt
     4321 tgattcgatg tgtcgcactt tcttcatacc aagaaagtcg ttgtgccaat aatgctttta
     4381 tttcttctct tgtttttcca agagctaaag gtcgaataga cgtttcatct tgttggatac
     4441 gatcaactag acagtccgct tctgcctcta agtaaagaac aatcggctgt gtttttaaaa
     4501 aacgccgatt ttctgcactt tggacaatcc ctccaccagt cgcgataatt ttgtcttcat
     4561 ttgacagctt tcttaaaata tctgactcca cttttcgaaa gcttttttca ccgtaatagt
     4621 gaaaatattc ggcaatcgac aattggatat gttcctcaat ctttgtatct aaatctacat
     4681 actctttttg taaacattga gctaaccctt taccgattgt tgtttttcca gcgcccatga
     4741 aaccaatcag caaaatgcta gccatgagct tctcctcctg acacaagttc tgctaaatcg
     4801 tcaaaaaatg ccggataaga gattgaaaca gcttcagctc cttctagttc gcaaggtcca
     4861 tcagctatca aagcagctac ttgcagcatc ataccgattc gatgatcgcc tctgctggac
     4921 acttttgcgc catgcagagg tgtcggccca tggatgatca gaccgtctgc tgtcgctttt
     4981 atctttgctc ccattttctg taattcttct gccaccgcat caattctgtt ggtttctttt
     5041 acttttaatt cttctgcgtc tttgatgatc gtgattcctt ctgcttgagt agccactaaa
     5101 gctagaatgg gcaattcatc aatcagacgc ggaatgattt ctccaccaat cgttgctttc
     5161 ttcaaatgac tcgtcttgac acttaaatca gccgattggt tctgttcatt gtgattcgtc
     5221 tgggtaatcg ctgcgcccat ctcctccaac acatctaaga taccagttct tgttggattg
     5281 accccgacat tttttaacaa cagttggctt tccggaacaa gaagtcctgc tgctagaaag
     5341 aaagcagctg atgaaatatc acctggaaca gttatctgtt gaccagttaa tttttgcggt
     5401 ccggtcacta tgatcgtttt atcttccact gtgatccttc cgccaaattg acgaatcatt
     5461 tcttccgtat ggttacggga acgttctttt tcaacgatta cggatgtgcc ttctgcttgt
     5521 aatgccgcaa acagcaaagc agatttgact tgcgcactgg ctacaggcat attgtatcgg
     5581 ataggtgata aagagcgggt tcctgtcaca gtgattggta gatactggtc attttcttct
     5641 ccttgcaagt cagcgcccat ctcgcgtaaa ggttccatca ctcgccccat tggacgttta
     5701 gacaacgaag catccccgaa cagagtagtc gtaaaaggct gtccagccaa aatacccatc
     5761 aataaacgta tggtcgtacc ggagtttccc atgtcaattg attcggttgg ttggacaaat
     5821 gagtggctac cgcgaccatg aatcttgatc acctcttcct cttcttcgat ctcgacacct
     5881 aattgttgga aggcatgtaa agtactcaga caatcttctg cgcgcaaaaa attttgtata
     5941 gtcgtcgttc cttcagcaat tgctccaaac atgatgctgc gatgggaaat tgatttatct
     6001 gctggtatcc taacagtccc tcttaatcca tgtatttgtt gtaataattg catttatcca
     6061 gcctcctatt tgatccagca atgataactg gtattagaca taatgattct ttttgcttgt
     6121 aacaaatcct gttggttttt aaaactgatt tgcagtattc ctaaaatatc ttccctggtc
     6181 tcttggatct tcaaattaat caaagaaact cccgcttttc cgatcagtcc agttacttct
     6241 gcaataacgc caggttcatc tggcacatcg ataaataaat caaaaaaggc aggaatcgcg
     6301 ccttgcttat ggacaggcaa ctggtctcga ctttcttttg cccgattgaa aaaagaaaag
     6361 atttccgatt ggttctcagt catgatccat tctgctattt gtgtcatttc ttgttgccaa
     6421 tctgccaatc gttccaataa tatctgctta ttgctcaaca aaatatctgt ccacatgaca
     6481 ggatctgagg aagcgatcct agtgatatct cgaaagcctc ctgctgccag ttgtttggct
     6541 cgcggatact cttgattgaa ttgttctgcc tgattgacta agccagctgc cagaatatgt
     6601 ggaaaatgac ttagcattcc tgttatacgg tcatgttctc ttgctgtaag agttacatat
     6661 tttgctttgg ttccgcaaaa tagttcttgc agtttttcaa catctttttt cgtcttttcc
     6721 aaaggtgtaa agatgtaata ggcattttca aatagtttag catctgctgc ctgtatacct
     6781 gatttatgag aaccagccat tggatgtcca ccgacaaacc gaagattttt ctgctcagcg
     6841 gatgtcacga cttgctgctt cgtactgctg acatccgtaa tgatgacgtg atcagacaag
     6901 ttgattcttt caaggacatc aagataagct aaggttgtac ggacgggaac ggctaataag
     6961 ataacgtctg cttgttctgc acctgtttca aaatcacttg caacgtaatc aacgatctgc
     7021 tgttctactg cttgctcacg ggtttttgac tgactatccc aaccaactac tatcagttct
     7081 ggattggtct gacgaataca aagtgccata gaagcaccaa tcagtccaag ccccacgata
     7141 aaaacatgtt gtgccatctt atttgccccc tttttagtaa tgtttcaagt agtcacggta
     7201 ctccttcact gcagctttca actcaacaag cgaatcactt gggaattttg acaatagctc
     7261 tgtcgctacc acagtggcta caacattttc cgcaacgacg ctagctgcag gaactgctgt
     7321 actatccgaa cgttctacac ttgctttgta aggttctttt gtatcgatat caacactttg
     7381 aagtggcttg tacaatgtag gaatgggttt catgaccccg cgaatgacaa gtggttcgcc
     7441 attggtcatt ccgccttcga agccgcctag attattggta cgtcggaaat agcctctttc
     7501 ttcttcccat aggatctcat ccatcacttc tgagcctggt aatgaaccag aagtgaatcc
     7561 gaccccaaat tctacgcctt taaaggcatt gatactaacc acagcttgtg cgattttggc
     7621 atctagcttc gtgtcccatt gtacgtagct tcctaatcca gctggaacgc ctccaacgag
     7681 cacttctaca attcctccga tcgtatctcc atttttcttt gtctgatcga tcaaatcttt
     7741 tattctttgc tcaactgctg gatcaacaac tcgtacttct gacgcttcag aaatttcttt
     7801 gatttcttga atcgagatat ttccaggtat ttctgcttgg attccaccaa gacaaatcac
     7861 gtgtcctgct acgatgatgt caagttcttc cagaattttc ttggcaattg cgccgattgc
     7921 tacacgcatg gtcgtctctc ttgcagagga ccgttcaagc acattccgaa gatctcgatg
     7981 atggtatttc ataccgccta ccaaatccgc gtgtcctgga cgcggacgtg caactcgccg
     8041 cagtgattgg tctttttcag gcacttctgc tacagacatg acagttgtcc aatttttcca
     8101 atctttattt tctaccacta aagtgacagg tgaaccaatt gttttgccgt gacggatgcc
     8161 agacgtgatc cgaacacgat ctttttcgat cttcatcctt cctcctctgc cgtaaccagc
     8221 ttgtctgcga gcgagttcgt gattgatttc ttcttctgac agaggtaatc ctgctggtag
     8281 tccttcaata atggctgtta attctggtcc atgggattct cccgcagtca tataacgcat
     8341 cttacagtcc tcctaagtaa tctttgaatg tctctgtggg aacaggaacg atttttgctt
     8401 gcccaatctt ttctagcaag atgatattca attggcttcc tcttgctttc ttatcatggg
     8461 ttatcgcgtg gaataattca tctggatgac ttagatcgta cgtaatcggc agatggaatt
     8521 tttgaagtat cctttctaaa gcagctgtcg atccagttgg agacaagccg attttttctg
     8581 catgagaagt gatggctacc attccaatag ctactccttc tccatgactg actaccccat
     8641 atcctgctgt attttcaatt gcatggccaa ttgtatgacc aaaatttaaa ataagtcgat
     8701 ttccttggtc aaagacatct tcttccacta cttttcgttt gatttccaac gtagcagtaa
     8761 tgattggaac agcatgttcc agaagatctt tttcgtcttt cagctcgttc agctgtttcc
     8821 aaagcgttgc atctgctata gctgctgatt tgatgatttc agcgattcct tctcgtaaac
     8881 gacgtaccgg caaagttttt agaacctcag gatcaatcaa tacaccagct ggctggctaa
     8941 atgttccgac aaggtttttc gctttgtttg tattgactgc tgtcttcccg ccaatactag
     9001 aatctacttg tgcaagtagc gtagtcggaa tttgaacgaa atggatccca cgcatgaacg
     9061 tcgaggccac gaatgctgct agatcaccca ccattcctcc accaagtgca agtatcccat
     9121 cgctgcgggt aaaatcatta tctgctaaaa aatcgtataa ctcagctgct tgttctaaag
     9181 atttactggt ctcacccgcc ggtacgattg aaaggactgg ttcaaaatta gcttgtctca
     9241 attggaacat gattttttct gcatatagtg gagcaacgtt tgtatcagtg atcacagcta
     9301 ctttttgagg tctccaaatc gtttgaagcc atgatccagc ctgttctagt gcgccttttt
     9361 caatatgaat atcgtatttt tggttatcaa catgtacagt aagcattcgt ttcacttctt
     9421 tcttttaaaa agctggaatt aatcgagtgt ctgaatcttt tccattgctt ctttcatgat
     9481 cgcaacttct ttcatcatgc gtgagtaagt cttttcattc aacgattggg gaccgtctga
     9541 ccaagcattt gcaggatcag gatggatctc tacgatcatg ccatctgctc cgcttgccac
     9601 acccgcacga gccattggtg gtacaagttc ccaaattcct gtaccatgac ttggatcaac
     9661 aatcacaggg aaatgagtta attttttcat caatgggaca gcacttaaat caaacgtatt
     9721 acgtgtcgct gtttcgtaag tccgaatccc acgttcgata aaaatgacag gagatttgtc
     9781 ttcgactgca atgtattctg cagcattaag ccattcattg atcgtaccgg aaattccacg
     9841 tttcaatcca acaggtttac ctgttttacc gacagcagat agcagtttaa aattttgcat
     9901 attacgtgcg ccaatctgta ggatatcgct gtactcagcc accatatcga tatgcccttc
     9961 atccatcact tcagtaatca ctttcattcc aaattcatca gccgcttgac ggatgtattt
    10021 caatccttct tcttctaaac cttggaaagc atatggagaa gtacgtggtt tgaaggcacc
    10081 acctcgcaga atttttgcgc ccccggcttt ggccattcgc gcacattctc tgatttggtc
    10141 taatccttcg atagaacaag gaccagccat cgtcacgaaa ctgccgtcac caatttttac
    10201 tccgtctaca tccacgactg tattgccagg atggaattct cgagaagtca atttgtatgt
    10261 gtttaaaata cgcacagcat tttctactcc ttcatagcgt gtaaatgctc cttcttgtaa
    10321 acttcgcgta tcacctttta gtccaatcac tactcgttct gttccttgat cgattgagac
    10381 tttgaatcct tctttttcga tatgatcgac gacttttttt atttgttctt ccgttgcttc
    10441 ttttttcata attactatca ttgtttttcc cgctttcttt tgtttactta tctatcttgg
    10501 ctgattttct ctactttttc aaggtggttt tacattaatt cctactaaat gaccgtgtcc
    10561 tgttgttctc attttcgctc cttctttcct acttatgacc cagataatca acaaaaaaag
    10621 atgtccctgt aggaaattgc ctctacagtg aacatcttca acgagcctta aaaagcgctg
    10681 caaaaatata aaatagccac tatagatgaa tttctctacg tggctgtaag tcgtgtttaa
    10741 aaatctttag ccatcataga tacaaacctg gtttgtttgt acccacgaag tcatgaatac
    10801 aaacctatct aaaatagcta aagtaatacg tattcagttt tgtaagtttt ctagatttca
    10861 taaaaaatcg ctccaaactg atgtaagaat tgtacctagt atagacataa aaaaatcgac
    10921 tgtcaatcat ttttatgaaa ctttttttaa ttttctgata gttaactttg tttttcagaa
    10981 aaataacaat tggttttatt gaagaaaata gtcatgaaca aaagtatctg agaacttttt
    11041 tactctcttg ttcatgacat atagaataaa gcagctttgt cttgtaaact ccgtttttca
    11101 aataataatc taaagattgc tactgaataa taaagccgat ttcatggtcc tcttcgctgc
    11161 tgattcggta aggtgactca acatccgctc cccagctatt gatacccccg actcctctta
    11221 cttttgcaaa aatcgtaaga accgttcgtc tttctggcgg tagttcttct atatgtgttg
    11281 cctgttccag ttcttctgct ttgaatggta aagcggaaaa agcaaatggc gtatcctgct
    11341 gttcaaatcg caacgaaaaa tcttcttgat ctttatccgt attatccaac gtttgatttc
    11401 ttgttagttc tacccatttc gtttccatat gcatgccgca ttcttgggga acaaggtatg
    11461 gggaaacggg gagtcctgcc actttataaa taccttcgac accaccagcc atccgatccg
    11521 gatatgtttc ccctgatagg ccttcatact caaaaaattt tgctttcgtt ggcataatga
    11581 accttaaacc caatgcgggt aattccggta atcctttttg tccataataa tgcatattga
    11641 caagcatttt accctctcca gcgatttggt aggaaattgt cacttgggtt tttggtactg
    11701 tgttgatttc cagtgtaaaa atgatttcaa ccgtttcagc gtattcatta cttgaatatt
    11761 gattatttac tggagcaatc ggcaaatcga tctttttatt gtccaccagt acttctattt
    11821 ctttgacttt tggatacaat ccggctccta accacatacc ggatttaaat ccaaatccgc
    11881 tacctctgtc attatcggtc gttgctcgcc aaaaaactgg aagtgtttcc cggtatagcc
    11941 attcttttcc gtttttattg agcgattcca gtccgcctct tgtataagaa aaaatataat
    12001 gcgtattccc cgatttgaca cctaatgtac agtcgccgaa gacgattgca actttttccg
    12061 tatccatatg acttttattt actgtatttt tcataataat atcttacctc ctgcatggcg
    12121 ggtttttctt gtccatctgc aaaaacgatc ccattccctg agaattcata gtcacttggt
    12181 ttatcgtcaa agtcaccgcc atagcgcatc acttctctgc ctgtgattgg atcagtcact
    12241 tggatcgcct gatctttaaa atcccatata tagcctcctt ggtacatttc ataacgatca
    12301 agcaacgtca tgtaagaatc cattccacct aacgaattcc ccatgtcatg catatattca
    12361 cataagatga acggtttttc cgctttattt tcaaggtagt caatgatctc ttctggtggc
    12421 gcatacatgc agctttttac atctgaaatc gaatcttcga atgctggatt tggaaaattc
    12481 ccttcataat ggacaagtcg atcggggtct acttttttga agtaatcgtt catttgtcga
    12541 atgttttctt ccgcataaga ttcatttcca agagaccaaa aaagaatact tggatggttt
    12601 tttaatgttt cgaaattcgt ttttgctcga tcaagaacag ccgcctgcca ctgaggtact
    12661 gcacctggta cgttgtaaga aggctcaaat gcgcccattt tttgccatga gccatgggat
    12721 tcgaggttcg tttccgccat cacatggatt cctgcttggt cgcataaata ataccaagtg
    12781 atctggttgg ggtaatgaga tgtccgtacg ccgttgatct ttgcattttt tatgatagcc
    12841 aaatcttttt ccatatcttc tatagtgatg gcgcgtcctt ttttcggatg ccactcatgt
    12901 cgatttaccc cgttgataat catccgtttt ccattgaagt agatgatttt atctttgatt
    12961 tcgattttac gaaaaccgaa ggaatagtca atatactccg ttactctttt ttcttcgtct
    13021 atcgtctcca attcaagctt atataaattg ggctgaaaat gcccccacag tgccacattt
    13081 tcaaatccat cgatgcgaat cgtcatttgc tcttttaacg gatattgctg ttgatagatt
    13141 tccttcccat caggatctag tatcgctaca ttcagctgac tgtttttggc tacttgctca
    13201 aaagacaaat ctacttcaag tttgccattt tgacatttgt catctaagcg aggtttgatc
    13261 caaagatctt ctaaatggtt ttgtcctcga gcaactaaga agacatctct gaagatccca
    13321 aaaaatcgga aaaagtcctg atcttccaag aatgcagctg tacttctttt gtaaacctcc
    13381 acagctagaa tattttcttc ttccaaataa ggtgttaaat caaattcaga cggagtgaac
    13441 gaatcttcgg catacccgat aaaatgacca ttcaaccaaa taaacattgc ttgttctaca
    13501 ccagcaaaat gaatcgagat ttgttttccc tttagacctt cttctaatcg aaaagtcgtc
    13561 aaatacgaac caaccggatt gtctttagca gctgaaaaga tcccttccgc ctctttctca
    13621 gggcttgtcg tgaaaggggg acggcgaaat acatgtccct cccacggata catcgtattg
    13681 atgtaatgga tcttgtcata atttgctaat tcaatatgac taggaacagt gatttcatca
    13741 aaagaacagg catcaaaatc ttttttataa aatgtttttg gtcgtaccga aggatttggc
    13801 gaataacaaa aacgccatga tccatttaaa gactgtttaa gggaggatgt ggtttctact
    13861 tcttcccttg ttgcataaca aacatgatcg ctatgtgcat ttgattggtt cactctaaaa
    13921 acttgtggat catctaacca ttttatattt gctttcatac ttttttcctt ctctctcata
    13981 actttttgaa atgaagcagt tctgtttgga aatcaccttg aaaatcgggg aagtaaagtc
    14041 caactgtcat caattcggcc cccgaaaata tccgcttcga ggtctgttcc tgataaagtg
    14101 cctcatcatc taaccccatc agatatactt catggaacgc tggttgagct gaagctagta
    14161 tcctccctaa aaacacaata gcttcatctt gtttaggaga cacaaacatc catgcggcaa
    14221 tattttcttc aaaaggactc gccaaacgat aataatggcc atactggatc accggacgaa
    14281 tcgtttgata ggatataatt tggtctttta cttgcttttc ttcttcagga gacagctttg
    14341 tcaaatccag ttcataacca aaaacagcgc tcatagctgt ttctcctctt gttttcaacg
    14401 aagtagatct gtcggtctga tgatttggta cagctgatac atgagctgtg atggacgaaa
    14461 tcggataagc aaggcttgtt ccatattgga ttttcatacg ctccactgca tccgtgttat
    14521 cacttgtcca tgactgcggc atataataaa taaacccggc atcaaaccgt ccacctccac
    14581 cagaacagcc ttcccataag atttccggat aatcagttgt caaatgttca agtagttcat
    14641 aaagcccgag catataacga tgcgctactt ctccttgtct ctgtgggtca agggtaatcg
    14701 aatagacatc tgaaagcgaa cggttcatat cccatttgat gtagtcaaga ggaatcgtat
    14761 ctaaaatctt tctcatttgt ttctcaatcg tctgccgaac atctaacctt gtaaaatcca
    14821 gtacatgttg tgatctggaa gcagaaggca ttctacctgg ctcctgcata agaaaatcag
    14881 gatgtgttcg atacagttca gaatcaaccg agatcatttc aggttcaaac cataaaccaa
    14941 atttcaatcc tttgctatga gcataatctg ctatttcacg taatccattt gtcaacttgc
    15001 cttcatactc gaaccagtct cctaatgaag aattgtccgc atctcttttt ccaaaccagc
    15061 catcatctaa tacgaacatt tcgatgccta gctcctttgc ttcatcaatg atcgccttga
    15121 ttttttcact attgaagtca aagtaagtgg cttcccagtt attgaccaga atcggccgtt
    15181 cagcaaattg atgacgacct cttgcgattc tttcacgtag caactcatgg tggatctgac
    15241 tcattttgtt caatccctga cttgagtaag aaaggatcat ttcaggtgtt tgaaaagact
    15301 ggcctgcatt caattcccac gaaaaacgat ggctgttgat cccaccgacg actctcaatt
    15361 gattgatctg atctttttca agttcgaaag aatggttgcc ggaataaaca aattgaagtc
    15421 cgatcgcttc tccagtattt tctgtcgtat gatgatgaac taaagcaata aagttattca
    15481 tatggtgact gctcgttcct cgacggcttt caaatacttt tgttccagat aaaacagatt
    15541 gccgtgaaat ctgtctttca ttgacatgtg ctcctggcaa agcaatgact tcatcaaatc
    15601 gcctagtatg ggcaaagtct agctggaaac tagccgcttt ttctagtttg attgtttctc
    15661 cggtttcatt tcttatcttt acagatcgtg tgatcactgc tctatcagta aatatggtat
    15721 agaacaaatc gaaataaatc gctgtttctc tatccttaag tgaaatcacc aacgtttgtg
    15781 cctctgactt gtccatcaca taagtttgag gcaagccttc gatctctggc ttcccatctg
    15841 taatataatg gctgtcatat ctcagatcca gtaagtctga tccattcttg cgctgaatga
    15901 tcgatgcggg cgttcgaaag tccatgctgc catgagaaga gtattcttgc ggtagtgtat
    15961 ctttcgacag agaccggtca ggatttaagg gaacatttcc tgaaaatcca cgatctcttc
    16021 ggggataatt tttattgtct ttgtactgct tgactggttt gccaaaataa atatgcgcca
    16081 atacattgct ttcttctatc tttatcagat aagaaagaaa tgtatttcgt aaatggaaga
    16141 ctcggctctt ttcatcaaat aagatgaaat ttttatctgt ttttctatgc gtaatatcca
    16201 tcatttcact cccaacaaaa cggaaaccgt ttactttttt ttataattcc tgtatactgt
    16261 ttactaaaca attttgtcaa taaaaatttt ttattttttt gaggagtgtg taaaaatggt
    16321 tggaattaga gatgtcgcac gaaaggcaaa tgtctctcct gctacagttt cccgtgtgtt
    16381 gaataacgat cagacgatcc atgtggctca gactactcgg caacggatag tagaggcagc
    16441 tgctgcgttg gattatgaca tcagtactcg taaatatatc aaaaagagaa tgccttcgat
    16501 tggtgtgatc tctacgatcg gcaaagcgag cgaagaacag gatatctatt acaaacattt
    16561 gcgagtaggg atggaagaag aagctcgacg tctgcattta gggatgaatc gaatctataa
    16621 tctatctgat aatcctaaac aatgggaaga tctagatcag ttaggtgcgg tgatcgttgt
    16681 tgggactgtt acgaaagaat cgattaccgc cttgttaaaa caaaacatgc aaatagtcgt
    16741 cgtggataac cctgacatcc agcaagaggt cgatatggtg tatgcagatc ttgaacggat
    16801 gactaaaacc gtactggaat tgttcttaga aaatggtcat aaaagaatag cctatatcgg
    16861 gggatataaa gtcgatatgg atgaattcgg tgggaagcag atttctataa atgaaaagag
    16921 gatgcgtgcc tatcagcatt ttatgatcga tcatcactta gaaaaatata tgtcttatca
    16981 tctaggtggc tggacagaaa aacaaggtga ggtacttgcg actaatctat tagaaaatga
    17041 atcccctctt cctactgcca ttcttgtcgg gagcgatcca atggcaattg gtgtctatcg
    17101 ggcattgaaa caaaaaaata tccaaattgg agaagagatt gttgtagcaa gctttgataa
    17161 tatcgagaca atcgcaaacc tctcccctgg cttgaccagt gttcaaatca atgcgaaatc
    17221 aattgggcaa gcagctgttc gaatggcatt ggagcgaatt gacggtatac gggacgaacc
    17281 aatcattttg acttatcctg cacgattgat tgttagggaa agttttatcc caagaacaaa
    17341 aaggtattga aagagaggtt gtgaaaagcc gcttcgtttc ttcatactca aaacgaaacg
    17401 gctttttcac aacctcttac ttatacttat tcacaaaatc caatgtctgc tggtaacaat
    17461 tttgcgattc ttttagcgtg taatcaaatt ggtattcatg ggtaatctct ttttttgtat
    17521 ctgtataaaa caaggattgc acgggtacct ttaattctgt caaccgattt tctaatgctt
    17581 gaccttgctc ctggaatgag tatgcatttc cgtcagttat atacgttggc ggaaattcct
    17641 tagaaacatg gtcagccaca ctagcttctt tcaattgctc actcgttttc caatcttttg
    17701 ttccaatcaa cgaccacgca acggttttga cgaaaaactt cataaatcga tcatccgaat
    17761 gctgctgtgc tgttagcttc aaatcaacag gtccgcaata agaaatggtt cctttcagac
    17821 tatcttttgg aagtagctgt ttgattttca tttgtttagc atacgcatca ttcgtttgta
    17881 tagctgcata ttgcaatgcg atttgtgaac ctgcactatc tccaccaaaa aataattgtt
    17941 ccatgtctag caatttgtcg tctttttctt cagctaataa atctttgatc agttcattca
    18001 cttgaagaac ttgattaggg tattctgaat caggcgctaa ttcataattc atcgcaacca
    18061 ctgcgatgtt ggcgtctgaa actaattttg tcgcaaattc tttgacaccc gatttatctc
    18121 cgccgacgaa tcctccacca tgtacccaaa atagaacagg aacaggcccc ttgctgtttt
    18181 ttggatagta gatgtcataa gtattctctt tatacttgga ctcatacgtc aaatctgttt
    18241 ttttcgccac gtttttttct gcttgatcga acgttttttt atcagtgatt tgaacagtat
    18301 ttgaaaacat atggttgatc aagaatgctc caggtctggg ggaaagctgg aaggcaagaa
    18361 agattgcaaa cgcgatccca agaatgattc cgaagaacca tccgataaat ttaagaattt
    18421 tctttttcat aaggctccct cagtagttag tattaagcat tctgattata ccatagtgtt
    18481 atttcctatc aagctctcct agatgattta atgattttcc ccaaacttct tctgcaatgc
    18541 ttttgacaaa agaaattttt tccattgttc ttcaaacaaa acattttctt cttctgttga
    18601 agcaaatcca cattgaggac ttaagcacaa acgatccaaa ggcagatatc gtgtcgcttc
    18661 gtagatccgg tcaatcactt gctggtgctc ttccatttcg ccatttttcg ttacagtcaa
    18721 aatctaagac aactaattta tcttcagaga ctttctctaa aagaacaaaa ctcccagcac
    18781 gttcactatc gtattctaaa taataggcac ggacattttc ttgagtaaaa taatgggtct
    18841 gctacgtcgt catcccccct gatgccgcca aaatcgaata ataatttccg cgtcaaacat
    18901 gtatatttag tgttgaatca tttgagaata tgaaaataaa aatctttttg atcaatcagt
    18961 taatcaaatt gaaataacga aaaatctgag acagaatgac gttcattctg acccagattt
    19021 gattttgcac aactcaacag tccttattgg caaatcgcca aaataatgta gactaactaa
    19081 aaagactaag aaacaataac aaaagttaac acttcttagt ctttctttag cacgtctatt
    19141 tattaaagat tttagggcct tttctcatgt ttttacgatc attcttaggt gtttccattt
    19201 gtttggatcg gcctccacct tgtttctttt tgatcaattc tttcattttt tctttttggt
    19261 ccatttttat tctcctttaa cgaatgaagc gactaattcg atatgatggg tttgcgggaa
    19321 aagatctaca ggctgaactt tcttcacggc ataacctgct tctgcaaata acttagcatc
    19381 tcttgcaaaa gttgccggat tacatgagat atagacgatt cgttctggtg acatttcgat
    19441 tgctgattca ataaaatgat gatctaatcc ttttcgtgga gggtcaacta cgatcacaga
    19501 tggtttgatc cctttttctt gccatttttt cataactttt tctgcttttc cgacttcata
    19561 atgaacattt tcgattttat tatttaaagc attgaactgt gcatcttcaa ttgcttctgg
    19621 aataacttcc atcccataca ccttatccac tctgtctgct aaagacaagc caatcgtacc
    19681 tattcctgaa taagcgtcga tcacaatgtc ttttttatct aactcggcaa actcgatggc
    19741 tgtttggtac agatactctg cttgttgagt atttacttga taaaatgact ttgctgagat
    19801 tcgataggtt tttcctaata gctgatcttc gatatagtct ctgccatata aaactttttc
    19861 tttgtctcct aaaatcacat ttgttttttc ttcattgata ttttggatga ccgaaaccac
    19921 ttctggcaat tcttcatgaa tagcttcagc aatttccttg gatttaaaga atttttcttt
    19981 acgagtaact aaaacgacca tcatttcatg ggaataatga ccacgacgga taatgatgtg
    20041 acgaagctgt ccttcatttt tttcttcatt atatcctcgt acttggaacc gttgcaagat
    20101 atctctgact actttgattg cttgatcgat gacaggatct tggatataaa aatcttcgat
    20161 agggactaga tcatggctat tctttttgta aaaaccggtg gctaactgtc catcgatacg
    20221 gcgaacaggg atctgtgctt tattacggta gccgaatggt tctgccattc cgaccgttgg
    20281 aagtacagga acttctggca tcttagcaat cttcgccatt acatgctcta cttgtttttg
    20341 tttgaagaca agctgttgtt catacttcaa atgggctaaa ggtgcgatac ctatgcgaag
    20401 taatgcattg ttttctacag gctgacgatc aggtgattgt gtaataaatg tttcgacttt
    20461 tccatatcca aacttattgc ctgcttttac tactagtatt tctacttctt ctccagggag
    20521 tgcattttcg ataaataatg gataaccatc gatttttgct acccccattc cttcataact
    20581 caagtcgacg atttcgactg tatatcgttc gtttttcttt actatttgct gttctgtcat
    20641 attttgctcc tttaagccta tttttcaatc ctactgaact ctactataaa tcagctaatt
    20701 acacaactaa aaccggcact gaccttacta cgttcagcac cggcttttga ttaattcttt
    20761 tgattaggtg tttcgtctgc tatttttccg tcaccgtcga tatcttcatc cgttaaacgt
    20821 tcgacttctt ttacgaattc tttgctgaca acctctaatt cttcttcatc tgtacctgtg
    20881 attgcttcat caggaatctg atcggtattg gcaaagaact caatgtgttg atgaagattt
    20941 tggaaatgca tcggtgcttc cccgccgtat tcaccatcta agttgatcat tactggtcga
    21001 ttgatatcat cttctgtttc aacgaacaaa ctgcttgttt tggtatagat tattcttgga
    21061 tcatcgacat gtcgaccgcc attcagcatg agtgctgcaa tatgcaagat ctcaaagata
    21121 tttgctgtct tcacaatgat taaagaaaac ttcccatcat ccaatttggc atctggcgcg
    21181 atctgttcga atcctccaac agagttcgtc agccctagga aaaacatcga agcatttccg
    21241 atatattccc cttcatcata cttcaagcgc attttgattg gctttactcg agggagcatc
    21301 tctgctcctt tcgcaagata agcaagataa ccaaaaatac tctttagttc tgatggaact
    21361 tcataagtca attctgtcaa atagccacct gcagcgatat tgatgaagta actgttatca
    21421 gaaaggccga tgtccatttt tacggtttgg tgttttttta tcacttctgc tgctgcttta
    21481 atattatctc taggaatttt taatgctcga gcatagtcgt tcgttgtacc agctgggatg
    21541 atggccattt taggacggtg ttcaagcgga gcgattccat taaccacttc gttgattgtg
    21601 ccatcgccac cagctgccac tactagatca aaacctagtt ctgcgactct acatgcttca
    21661 ttcttggctg aatctggttc tggtgtagtt gcataggcgc tggcttcaaa tcctgcttct
    21721 tctaatgcct gtaggatatc cgccaaattt tttttcaata attcttttcc cgaggtgggg
    21781 ttataaatta cgcgtgcttt tttcattttc agcctctatc gtttcgctaa ttcatcttta
    21841 agtagcttat tcacgacacc aggatttgct tgtcctttag ttgctttcat gatttgaccg
    21901 actaggaagc caaccgcacg atcttttcca tttttgaaat catcgacaga ttgttggttg
    21961 ttatccaaaa tttcattaat gattggcagt agtttgttcg gatcagataa ttgcacccaa
    22021 ccattttttt caactacttc ttgggcttct ccgccatttg tgatcaattc acggaagact
    22081 tttttggcga tttttgagct gatcgttcca tcggcgatca aacgaatcat tcctgctaag
    22141 ttctcaggtg tcaattttgt ctcagctaat tccagtttct cactgttcaa gtaagcagaa
    22201 acttcaccca tcaaccagtt agatgcttgc ttggcatctg caccattttc caaaacagct
    22261 tcaaaaaaat cagacatttc ttttgtaagt gtcaatacca ttgcgtcata ctctggaagt
    22321 ccaaactcac ggatatagcg ttcgcgacga gaagctggca tctcaggcaa actagctttt
    22381 actttttgaa tccattcgtc atcgatcacg aatttcggga tatcaggctc tgggaagtaa
    22441 cgataatcac tagacccttc tttgacacgc atcaaaactg ttttattcgt tgtttcgtca
    22501 aaacggcgtg tttcttgttg gatttctcct cctgaaagaa gaacttttgc ttgacgtttt
    22561 tcttcaaaag ctaaaccttt tttcacgaaa gataaagagt tcaagttttt caattctgct
    22621 ttcgtaccga actcttcttg tccataaggg cgaagagaaa tattggcatc acagcgcata
    22681 gagccttctt ccattttcac atcgctgact tctgtaaatt ggataattga tcgcaatgct
    22741 tctagataag cgtatgcttc ttctggtgaa cgcatatcag cttcagaaac gatttcgatc
    22801 aaaggcgtgc cttgtcggtt caaatcaacg taagaataac cgccaatacc atgcatgttt
    22861 tttccagcat cttcttctaa atggacacgt tcgatccgga tttttttctt tttaccttct
    22921 acttcgattt cgatccatcc gtcatgaccg attggttgat caaattggga aatttgatat
    22981 gcttttggat tatctgggta aaagtaattt ttccggtcaa aatgagtatc tttagagatt
    23041 tcacagttta atgccaaagc tgctttcatc ccgaattcca gcgcagcttt attcattact
    23101 ggcagcacgc ctggataacc ccagtcgatc acgtttgtat tgctgtttgg ttctgcacca
    23161 aaatgagctg gcgctggaga gaaaattttt gagttggttt ttaactcgac gtggacttct
    23221 agtccaatga ctgtttcaaa attcattcgt ctttccctcc taaaatagct ggttgttgtt
    23281 tatggaattc tgttgcttgt tcaaacgcat atgccgcttg atacatcgtt ttttcatcaa
    23341 aataattacc gatgatctgc aatccaactg gtaatccttc tgataaacct gccggtacag
    23401 acatacctgg aagtccagcc aagttgacag ggatcgttaa gatgtcactc atgtacattg
    23461 tgattggatc gttaatgttc tcaccgattc cgaatgcaac tgttggtgaa gatggcccga
    23521 tgatcaagtc ataatctgca aagacttttt caaaatctcg tttgatcaat gtacggactt
    23581 gtcctgcttt tttgaagtaa gcgtcataat aacccgcgct taaggagaat gtacctaaca
    23641 tgatacggcg ttttacttct tcaccgaatc cttctgaacg tgagttgaca tatacatcat
    23701 ctaatgattt cacattttca gagcgataac cgtagcggat accatcgaat cgttgcaaat
    23761 tggaacttgc ttctgatgag gcaatgatat aataaacagc cacaccgtat tttgaatgag
    23821 gcaagctgac ttcttccaca acagcaccta atgaacggaa tgtttctgct gctttcaata
    23881 cagcttcttt tacgccaggg gccacacctt cgcctaagta ttctttaggt aaagcaatct
    23941 tcattccttt gatgtcgcct gtcagtccct ctgcaaaatc aggaactgaa acgcctgaag
    24001 aagtaccgtc tttttcgtca taaccgctga tagctgtcaa tgctaatgca ttatctttca
    24061 ccgtacgggt cattggtccg atttggtcaa gagatgaagc aaaggcaatc aaaccaaaac
    24121 gtgagacacg gccataagta ggtttcatcc ctacgatgcc gttaaaagct gctggttggc
    24181 ggatacttcc cccagtatca ctacctaaag aaactggaat ttgtccagct gcaactgctg
    24241 ctgcagaacc accagaagag ccaccaggta cttttgttgg gtcccaagca ttttttgttt
    24301 tcttgaaata agaagtttct gtacttccac ccatagcaaa ttcatccatg tttagcttac
    24361 caacagggat catatctgct tgatagattt tatccatgac cgttgcatca taaattggtt
    24421 caaaattgta aaggatctta gatgcagcgg ttgtcaagat atcttttgtt acgatattat
    24481 ccttgatacc aattggaata ccagcaagga cgttgctttc atcaatccct ttttcatcta
    24541 aagcttttgc ctgttccaat gctttttctt cgttcaatgt aatgaatgcc tcgacctcag
    24601 gctctgtttc tttgattcgt tcaaatgttg tttttgtcaa atcttgtaca gtgatttctt
    24661 tcgatacaag catatgatgc agttcttcta gagaacgatt gtgtaattct gtcattatgc
    24721 gccagcctcc ccattatcga tgattgcggg tactttgata aagccgtctt ctgtttccgg
    24781 cacatttttc aataattcat cgcggttcca tccagcttgc gcccgatctt gtcgcatcac
    24841 gtttactgtt tcaaccacat tcgacgtaaa tggaacacct tccgtatcta cttcttccag
    24901 catttcaacc atatcgatga ttttacctaa ttggtcggta aaacttgcaa gttcttcatc
    24961 tgcgaacgca agttttgcta atttggcaac gtgtttcact tgttcttcac taatcgccat
    25021 actacccctt ctttctaaac gagttaaaaa ctttgctctc tattttaaca caagagtgcc
    25081 gtgtctgaaa ttgtttattg aaatttgtct gaaaaaactc atttttcatt cttctactga
    25141 gcgactttta gccaatgcat ctgccatttg atcttctgtc caaacttcga ttcctaattc
    25201 ttgtgctttg gtcagcttgc tgccagcatc ttctcctgct accacgatat ccgttttttt
    25261 cgatacgcta ccagtcactt tcccgccaag attttcgatc gtctcttttg cttcttcacg
    25321 agtaaatcgt gttagtttac cagtaaggac tactgttttt tctttaaaag gagactcgac
    25381 ttcttgcaat tgggcatttc gaagcccttt gtattcgaaa ttgacgcctg cttgttttaa
    25441 ttcattcatc aattcatgga cttcttcgtt actgaaataa gtgactacac tgtcggcaat
    25501 gatatcaccg atcgtatcaa gcgcaataat cgactcatag tcacttttac tcaatgtttc
    25561 tagatcaccg aaatgttctg ctaaaatttt tgctgctttc gctccgacat gtcggattcc
    25621 taacccgaaa atcaaccgct cgaccgaatt atctttgctg ttgtcgattg ctgtcaaaat
    25681 attatttgct gatttttctt ttattttgtc caacgtcaat aactcttctt ctgtcaattt
    25741 gtaaagatca gcaacatctg ctactagttt tttatcatac atctgttcta gtacgcgcgg
    25801 ccccaaccca tcgatattca tcgcatttct tgaaacaaag tggttcaaac cttctttcat
    25861 ttgagcaggg catttaggat tgatgcaccg taaggctact tcttcatcca aatggacaag
    25921 ttcacttcca caaactggac aatgtgtagg caattggtat tcttcgctgt ctttaggtcg
    25981 tttatctaac acgacttgcg aaacttctgg gatgatgtct cccgctttat aaatcagaac
    26041 tgtatctttc aagcggatat ccttcaactt aatataatca ccgttatgca ggctggcacg
    26101 gcttactgtc gttcctgcta ctcggactgg tgtcatgata gctgttggcg ttactacccc
    26161 tgttcgaccg actgtccatt cgatgttttc gattagcgtt tcaacttctt ctggcggaaa
    26221 cttgtaagct gctgcccatc tcggtgcttt gatagtaaaa ccaagttgat cttgtagcga
    26281 gaattcattc actttgatga cgattccatc gatttcatag ggcaaatcca cacgtttgtc
    26341 atgatattct tcaatgtatg accacacttc atcaatcgaa tgacatagac gtttttcccg
    26401 attcgtgtgg aatccaattt tttctagctc ttctagcgca tcatactggg tttttgcttg
    26461 cataggaccg aaatcagcta ctgtgtataa aaaagtgtcc agatttcttt tcgctgtgat
    26521 tttagaatct aactgacgca aagaaccagc tgcggcgttc cgaggattcg caaagatatc
    26581 ttttccctct gcttctctgt cttggttcaa ttggacaaat gaccgttttg gcatgaaaca
    26641 ttcgcctctt acttcgatgt tcattggttc ttttaatttg attgggaccg accggacagt
    26701 ttttaggttt tcagtgatgt tttctcctac agttccatcc ccgcgagtcg cacctctcac
    26761 gaaatttcca tcttcataac gcaaagaaac ggaaagtcca tctattttta gttcacagca
    26821 atagtcaact acacgtccca ctgctttttg tacacgttga tcaaaagcta tcagctcttc
    26881 tttgctgaaa acgtcgttca aactgtaaag cggaatatca tgagtgactt tttcaaaacc
    26941 ttctaatact tttccgccta ctcgttgagt gggagaatca gacgtgatca aatcaggata
    27001 ttgttcctcg attgctacta attctgcgta ttctttatca taaacatagt cttccactgt
    27061 tggtttatct tctacgtagt attcgcgaga ccattggttc agtcttgtac gaagttcttc
    27121 tgctcgttta gcagcttcgt caatcgtcat ttcatgttcc atcatttacc ctccactact
    27181 aagctttttc aattggcgca aaggcagcca aaagacgttt tactccttga cttgggaaag
    27241 ccacatctag ttctagatcc ttcgacgtcc cgcttactcg gacgactgtt cctagacccc
    27301 attttttgtg tttgacttta tctccaggtt tccagctgtt attttcccct ccagtagcac
    27361 ttttagaggg aacaaccgct gtctctggct gtttatagct atatgctgtt tttttagcga
    27421 atacaggcgt ttttttaggc gtaggacgca tgccttcgat ttccagtaac tcctgatcga
    27481 tttcttctac aaaacgagaa ggtcggttat attgcgtccg tccatataat gttctggaga
    27541 aagcatttgt taaatacaac gcttcttctg ctcgcgtgat ccccacatat gcgagacggc
    27601 gttcttcctc cagttcgctt tcttccatca atgctcgaga taaaggaaaa accccctctt
    27661 ctagaccaat caggaataca actggaaatt ctaatccttt ggctgcatgc aaagtcatca
    27721 atgtcacttg ggaagcatct tcttctaaat tgtcaatgtc ggaaacaagt gccagatcat
    27781 ttaagaagac cgttagtttt tcttctggtg catcagcgtc ttcttcattt tgctgttcga
    27841 attgtttgtc gaattcttgt gtgaccgtta aaaattcctc caagttttcg atgcgtgctt
    27901 gtgcttctaa tgtattttgg atttttaagt cttctagata tccgctacga tccaaaacct
    27961 ctttcgtcaa ttctgtcact gtcaagtatg ggatcatctg agtgacttct tggatcatct
    28021 ctccaaatgc acctaattgc tgtcctgctt ttccaccgat atttgccaga tccacatttt
    28081 gcgctgcttc aagcagtggc cattcatgaa gagaagcaaa actgcgtagt ttttcgatag
    28141 atcctggtcc gatgcctcgt ttgggcgaat taacgatacg ctcaaaactg attgaatctt
    28201 gaggatttac cagcacattc aaatacgcta aaatatcttt gatttcttta cgatcgtaga
    28261 acttgtgccc tccaaccatt ttataaggaa tatttgcttt caacagagtt tcttccatta
    28321 cacgagattg tgcgttcgtc cggtacaaga tggcaaagtc gccgtaattc cgatgattgc
    28381 ttcgaatctc ttcttgcatc cggtctacga taaaacgggt ttcgtctcgt tcattatctg
    28441 cacggtagta agtgatcttg tttccttctt tgttttctgt ccacaagttt tttggttttc
    28501 gattgctatt gttttcaatg acttggtttg ctgcgctaag gatgtttttc gtggagcgat
    28561 agttttgttc tagcaaaatt actgcagcat ctggataatc tttttcaaaa tccaagatgt
    28621 tctgcatatc tgcaccgcgc cagccgtaaa tactttgatc tgcatctccc acgacgcaca
    28681 ggttcctaaa tctacctgcc aataaattga ctagcgtata ctgtgcgtga tttgtatctt
    28741 ggtactcatc tacatgaata taatggaatt tgttttgata atacgtgagt gaatcaggat
    28801 gttcttcaaa taatcgaatc gtattcatga tcaggtcgtc aaagtccata cattgattat
    28861 attgaagctc tttttggtaa gcagcataac attttgccgc aatctcttcg taataactgc
    28921 cttgcatttt tgcaaaatcc tgaggggtct gcaggctgtt ttttgcttga gagattgttc
    28981 ctaatatact tcgaggatca tattttttcg gatcaatgtt cagttctttt agtatccgtt
    29041 tcatcaaggt cagctgttca gaagaatcga tgattgtaaa gttccggtta tacccgataa
    29101 aatcaacatc tcttcttaaa atacgcacac acatcgagtg aaaagtcgat acccagacat
    29161 cttcgcctcc agatgcgaga atcgcattga cacgttcttt catttctttt gcagccttat
    29221 tggtaaaagt aatcgctagg atgttccaag gattgacttc tttttcctca atcaaatagg
    29281 caatgcggtg tgtcagcact ctcgtctttc cacttcctgc acctgccatt agcagtaatg
    29341 gtccgtcagt gtgaagaaca gcttctttct gacgggggtt catcccgttt aataattcag
    29401 ctttggatga cattaacaac atcctttctt ttatcaatct tgttaattat agcattttct
    29461 tggctttcat taaactagct agttcaaaat catttgtttc tttttatgga tttttccttg
    29521 tcaattcgtt gatttcgcgc taaattaaaa gaaagcgagg cgtttgaatg gaaaaaaaag
    29581 gaaaaatcaa gaaattagat aagactcaga tatattttat tctaaaagga atatttgttg
    29641 gactgctgac aggaatcgtc gttagcctgt ttcgattatc tatcgaaaag ctaagcgatt
    29701 tagttcgaag tatctatgaa atgagtcggg aacagccgat ttacttaatt ggcctagcta
    29761 tttgctgcat ccttgcagca tttttcgtgg gttatctggt caaaaatgaa ccggatatca
    29821 agggaagcgg gattccacaa gtagtgggac agcttcgcgg ggaattatca atgaactggt
    29881 tctctgtttt atggaaaaag ttcattggcg gtgtactttc agttggtgcg ggcctttttc
    29941 taggtagaga aggaccttct atacagcttg gcgcatccat tggacaagga gctggacaat
    30001 tgtttcggag tccatcttca gaagaaaaga ttttaatttc tagcggtgca agtagcggac
    30061 ttgcagcagc attcaatgct cctatcgcag gattactctt tgttttagaa gaaatccatc
    30121 actccttttc tccacttgtt tggctgacat catttgcttc agcaatcact gctaacttcg
    30181 tctctcttta ctttttcggc ttgagacctg ttttatatct aggtgacctt ccttctttgc
    30241 cgttgaaata ttatggttcc ttagtgatct taggagtact tctaggcatc ttaggtttta
    30301 tttatcagaa tgtcttgctg gctttgccaa aattttacaa ccgattgcct ttaccagctt
    30361 attttcacgg gttcgttcct ttcttgctga ttttgccaat tggttattta tggcctagta
    30421 ctctaggcgg aggcaatcaa atcgttttag cttttggaca tgcttccttg ccaatcatta
    30481 tgattatcgg tttgtttgtt cttcgattcg ttttttccat gatttcttac ggtgggggtc
    30541 ttccaggggg gattttcctt ccgatattga cattgggggc tttaatcggt agcttttatg
    30601 gaaacattat ggttgatttc gggatggatc caatctacat caaggatttt gtcgttattg
    30661 ccatggctgg ttatttcaca gcgattggaa aagcaccgct gacagcaatt attttagtga
    30721 cagaaatggt tggctctctt aatcatctga tgcctttagg attggctgct cttgttgctt
    30781 atatcgtcaa tgaccttctc ggcggtaatc caatttacga atcattattg gaacgtctac
    30841 ttggaaatac tcagaccgcc tcactaaaag gtgcaaggac aacttttgat tttccagtaa
    30901 cggcagagag ttctttagac ggattaatgg tacgagactt caattggcct aaaaatatgc
    30961 tgctaatctc tatacgcaga ggaaatcagg aaatcttgac tcacggtgat acgatcatga
    31021 atgtaggaga tattttggtt atcttgactg atgaagcaaa cttgccaaga atcaagcagg
    31081 aaatcgaggc taaatcttct atcacaaatt tgaagcaatc attttaacga acatggaaaa
    31141 actattttac aacaactatt catctacact acttgattct atatgattct ataaaaaaag
    31201 aagaggctgg gaaaacccag cctcttcttt tttatttcta tgcttctgct actgcaggag
    31261 cttttctcaa taaaccataa atcacaccag atacgactgc tccgatagca ataaacacca
    31321 aatacatgac cgggtgactc agtaagaata caacgaaaat cccgccgtga ggagccagta
    31381 atttgattcc gaaagctccg actaatccgc cagttaaagc agaaccaata ataaaacttg
    31441 gaatggcacg aatcgggtca gctgctgcaa atggaatcga tccttccgtg acaaacgata
    31501 gtcccatcac gatgttcgtc aaaccagcat cttgatctgt tttgctgaat ttattcttaa
    31561 acaaacgtgt agcaacgaaa attgccaatg gaggtaccat tcctccagcc ataacagaag
    31621 ccatcacgac acttccgcca gttgcaacag tcgtagctaa agttgctgta ccaaacacat
    31681 aagcggcttt gttgattgga ccgcctaaat ctgccgccat cattccagct aataatgcac
    31741 caagtaaagc agcattcgaa ccactcatac ttgtcaagaa accgttcaat gcatcattga
    31801 ttgctttcat agggacattg accagcaaca tcaagaaacc agtaatcagt aagccaaaca
    31861 ctggataaaa gaggatcgtt ttaattccat ccaatgattt tggtaagttc ttgaataatt
    31921 tacgtaaaaa tacgatgaca taaccagcta agaatccacc aatcagtgca cctaagaatc
    31981 ctgcaccgcc tgtattagct aatgcaccag cagcaaatcc aacgatcaaa ccagggcggt
    32041 caccgatact tgaagcaata aatcctgcta ataccggcag catgaaacca aatgcagctt
    32101 gtccgatttg gttgaaccag ctagcagctt gattgtaatt ccctagattt gataattgat
    32161 catgtggcac gccaataaat tggtcgatca tgaaagaaag cgcaatagcg attcctccac
    32221 caataacgaa aggtaacatg tgtgatacgc cgttcatcaa atctttgtaa attctttgtc
    32281 cgatagtacc atccgcattt ccttcttctg aactgctttc ttttccatca ccatggaata
    32341 ctggtgcagt accactaagg gctagattga tcaactcttc tggtttacgg ataccatcac
    32401 tgacaggacg attgatcagt tcttttccgt caaagcgatt catctctact tttttgtctg
    32461 ctgcaacgat tacaccttct gcacgggcga tgtcttcagc agtcaatcgg tttttgattc
    32521 cttctgaacc gttcgtttcg actttgatct ccacacccat ttctttcgct tttttcttca
    32581 aagcatcttc tgccatgtaa gtgtgagcga tccctgttgg acaagctgta acggcaacaa
    32641 cgaattttct ttctggatga gcagaagctt ctgcagcttg tgtttcttgt tctgcttttt
    32701 cttctgcttc tttttgctct tcagctgttt ggaacaagtc ttggacttct tctggtgtat
    32761 ttgcttcttt taattttcct acgaaatctg catcaatcag caatcgagat agtgctgcta
    32821 atgcttgcaa atgtgtgtcg tttgcgcctt ctggagctgc gatcatgaaa aacagataag
    32881 ttggctgtcc gtctaaggct tcataatcta ctcctttttg gctttttgcg aataaaactg
    32941 ttgcttcttt aactgcactg tttttcgcgt gaggcatggc gattccatca ccaagacctg
    33001 tagaagtttg tgcttcgcgg gctagaattc cttctttata tgtttcgata tcggaaatcc
    33061 ggccggcatc aaacattttt tggaccattt catcgattgc gcctttttta tcagtcgctt
    33121 gcaaatccat gatcatcgct tctttgacca ttaaatcctt gatattcatg attatcctcc
    33181 ttgaaactaa tcaatttttg tgatttcaac ttctggcagc aactcatcaa tcaattcgcg
    33241 agctgccaag tcatcagaaa aagctgttgc gcttccgcaa gcaactcccc atttaaaggc
    33301 ttcgattgga tctttcgtac ggctgtaagc cccaataaag ccagcaatca ttgaatcccc
    33361 tgcacctact gaatttttca atgggcgaac taatacattc gttttataag ttccttgttc
    33421 cgtgaagagc aaagcaccgt ctccagccat tgagatcagc acattctgtg ccccttcagc
    33481 tagtaatttt ttcccatacg gaatgatttc ttctacagat tggaaggttg tgttgtaaag
    33541 ctcagctaat tcgtgattgt ttggtttgat caacaacggt ttcttaacaa gtgcattttt
    33601 taggtcatca cctgttgtat caatcacgaa atctgcacct ttttctcgaa tgatatcaat
    33661 cagttcttga taaaatcctt ttcgcaggct agctggcgcg cttcctgaca atacgacgat
    33721 atctccttgt cctactgttc ctaatgcttg cttcaactca tcgatttctt catctgaaat
    33781 agatgggcct aagccattga tctcagtttc agtgtctgat tttagtttga tattgattct
    33841 tgtatcttct tttacggttg tgaacaaaga ctggatttgt tctttttcaa gccacttcga
    33901 aataaattcg ccagtgaacc ctcctaaaaa ccctagtgct gtagattgag aatcgatccg
    33961 tttcaaaata cgggaaacat tgatcccttt tcctccaggt aacttgaagt cttccttcat
    34021 tcggttcaaa tcgcctagtt ttaacccatc tacgcgtacg atataatcaa ttgatggatt
    34081 aagtgttact gtgtaaatca ttgtattgcc tccttaattg ttgtcttttc acggaattca
    34141 ttaacgaatt ctaatggaca acgctcggtt atgatcgtgg ctgcttccaa aggtgcaaca
    34201 gaggtgaagg acaactggtt gaatttggta tgatcaatca atacaaatgt ctcttcggaa
    34261 tttttcatgg ctaaacgttt aacagctgcc tcttctggat ccggagtcgt gaatccttgt
    34321 tcaagatggg caccgttcat tcccataaat gctttattaa aatgaaaatt ttttagttgt
    34381 tctatcgcat tagatcctaa aacagcatta gttgccagct ttatcgttcc acctaaaata
    34441 atggtagcaa gctccaaatc aactaaattt gccgcatgtt gaacggaatt tgtcactact
    34501 gtgatctgtt ttccttgcag aaaaggaatc atttctaacg tagtagagcc agcgtctaaa
    34561 taaatgacat cttcgtcatt aatggtttca gctgccgatt tggcgatttg ctgtttttcg
    34621 tgaacgtttt tgagtgattt ttcactcata ttctgctcaa aattcaataa ttgctctttc
    34681 ttagctccgc catgaactct ttctagaaac ccactattct ccagctcttg taaatcacgt
    34741 cttatcgttg attcagaagc attcaaaagg tttacaagat cttgtgattt cacgattttc
    34801 tgctgttcaa gcaagtctag gattttttgt ctgcgttcct ctgtaagcat tttcacgatc
    34861 acctcaatac aaataatagc atattttttt tctactagca atataaatca gtcaaaaacg
    34921 ttcataaaaa ttataaatca gtcaaaaaaa aataaaaaac cagtcagaaa gactggtttt
    34981 ttattttttt gtctatcatc aagccgtttt atcgtttttt attttcgctt cttgtttttt
    35041 tcatataaac agaagcaggt tttctttcct tatgaagaag ataataataa agtcccatcc
    35101 cgccaaacat gaccaaaaaa ccgatgactg atcgaatagg aaaattagga acaaaaaaga
    35161 gacagacgga taataatgat gccggaacca ttattcgata atagagttgt ttatccattt
    35221 tcttcacctc atattttttg taaagtaatc aattgttcta attctcggta atctaaaaat
    35281 tgcacacgca gatgctgctc acctttcttg acatcgaatt ggatacttcg ccgctttttc
    35341 tttattcgtt tgaaggtaac atcagagagt ctatgtttga tttcctttaa cggtaattct
    35401 gacagggatt ctgaaggaac atagattctt aaggataaaa gatactcttg atatttttca
    35461 tctgttacct ctacttctag atattcccta atataataac aataagcttc gattccatta
    35521 tccaaagcaa cagtcggatc gataacttta tagcacatca tgcgtgatcg attctcgtca
    35581 acccaaggaa aatacgcctg agcatttagt gttccgcgaa cacgcctttc tttataatct
    35641 ccttgttgga tatcataact ttttgttaca aaaaatgaag cattcttttt aggaacaggt
    35701 tgcatttgtg cattctgata ctggacagat tggatcgtat ggtcagtgta aatgtattca
    35761 acgaatggcg gtttcaataa accgacacca ttgtcccaag caagatgatc aaacccaagc
    35821 tggtccctaa aataggattg aaacatttga ttgtttggct tttgccagtt aaaggggtct
    35881 cgataaagct ggatacatat gacttcgttt tctaattcct tcactttttc tacttgttgg
    35941 atgtcgagaa aaatcggttt agggatgatt tgatgataat agcgactata atacatttcc
    36001 caacacgaag tgaaacataa accttggtaa aaaagatcgt atcctggaaa ttggttgcaa
    36061 tcgactacta cttccccgtt ttgttgtttc attttaggta aatgttcgat ttcatccttt
    36121 ttctcaaaca atagccgttg agagatctgt tttgtattgt gatagataaa ctcgctataa
    36181 gctcgcatat aggcgaacac cccatgagca atcatttttt tctgaatgaa gtctcgaatc
    36241 aaaaattgct tttctttgaa aacagagata gacaatacat tttttatcaa taattgatta
    36301 tcttccaagc gaaaactaca ttcgttctcg tcatttccta caactaacca actttcgtct
    36361 cttaccgatg ctagcattat tttttcttga agctttcgaa gatcatacgg atccgcttgt
    36421 tgtgtgaaag agcggataaa tgttggctgg atcaacagac gtgtcaaaat agcccaatcg
    36481 cttatttcct gttttttttc ttcttcattt aagtctgtga gctggatagt catcaataat
    36541 ttatcttcgc caatcatttt ctcacct
//
LOCUS       DACDUU010000023        35369 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.023, whole genome shotgun sequence.
ACCESSION   DACDUU010000023 DACDUU010000000
VERSION     DACDUU010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.023"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..264)
                     /locus_tag="IWL30_001153"
     CDS             complement(<1..264)
                     /locus_tag="IWL30_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9705935.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLHKPDLTFHELVEEWLVIYKRQVKE"
     gene            complement(380..946)
                     /locus_tag="IWL30_001154"
     CDS             complement(380..946)
                     /locus_tag="IWL30_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705936.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWLLAVVVVFIAIGSQGGSD
                     DAKNTVAETTKESVTEVSSAESVAESTVVEEETETTETTIEEVTQEESVPREYRNALS
                     KAESYLGWAGMSEQGLREQLEFEEYPSDAIDYALANVDVDYNEQALAKAESYDDWASM
                     SDSQLYDQLIFEGFTNEQAQYALDNLPQ"
     gene            complement(1082..1735)
                     /locus_tag="IWL30_001155"
     CDS             complement(1082..1735)
                     /locus_tag="IWL30_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705937.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDTTSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQATAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            complement(1785..2213)
                     /locus_tag="IWL30_001156"
     CDS             complement(1785..2213)
                     /locus_tag="IWL30_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP9705938.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRDRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIEELKNLYGLPESAYRFLI"
     gene            complement(2218..2592)
                     /locus_tag="IWL30_001157"
     CDS             complement(2218..2592)
                     /locus_tag="IWL30_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9705939.1"
                     /translation="MSLVTKIKELADEKHVTIAEVERQVGISNGQIRRWDKASPKSEN
                     LKKVADYFGVTTDYLLGNNNVPKWATKEEVVELDKLLDSNVNMSYGGETLTPEQIQRV
                     KDILIATFWDIVKEDKEKGKKM"
     gene            2896..3081
                     /locus_tag="IWL30_001158"
     CDS             2896..3081
                     /locus_tag="IWL30_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9705940.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            3094..3342
                     /locus_tag="IWL30_001159"
     CDS             3094..3342
                     /locus_tag="IWL30_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705941.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            complement(3645..4103)
                     /locus_tag="IWL30_001160"
     CDS             complement(3645..4103)
                     /locus_tag="IWL30_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705942.1"
                     /translation="MIKILSDYQVAITLIISISGFFLSLYNLFKDKRKITLSYFLVKH
                     DRSNRMILTGVIANPSKMPNSIIECTYLYNGKKINCSHYHANGFDMGSIHKSSLLSPI
                     PLAQAISPGYSVAFSEVLDMKDILPGEKLVMVIRTANYQKKFKLKLKDSF"
     gene            4271..4588
                     /locus_tag="IWL30_001161"
     CDS             4271..4588
                     /locus_tag="IWL30_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705943.1"
                     /translation="MLIATDTLDRIFLKDEYKLRNIDASGILVFDLYDNGKIGIYQAS
                     DIEETNLAFEQIDDSVELDLDEAILAFEQIAKLLKEAQKNGNSLPTQRVIYQSPGTGR
                     TIG"
     gene            4521..5000
                     /locus_tag="IWL30_001162"
     CDS             4521..5000
                     /locus_tag="IWL30_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HAP9705944.1"
                     /translation="MATLYQLSESYIKVLELAEQLDEEILRDTLDSINEAIEYKAENL
                     AKIVKEVEGKAELIDSEIKRLQERKTSLLNNAKSIKHYLQEEMEKTGKTKIKGELFNI
                     GIQNNPVSVNVINENLIPKGFFTPVPPKLDKKQLKEELKHGDIPGAELVQTKGLRIR"
     gene            5450..5881
                     /locus_tag="IWL30_001163"
     CDS             5450..5881
                     /locus_tag="IWL30_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705945.1"
                     /translation="MKNKLAKTTAIIGLALGSGVIGYAASNAFQDLDTIKANFNTVLQ
                     YGQTKSQRVSELESQLSNNTRTQEQLKAEIEQIKSDKQKEIEAKQREVEQKQQEIATK
                     QQEADSLRQQLSTVQNDKEQLEQRVSELRQYTDQKVGELGK"
     gene            5878..6204
                     /locus_tag="IWL30_001164"
     CDS             5878..6204
                     /locus_tag="IWL30_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP9705946.1"
                     /translation="MKELTIYDPNISWAKHTIKVSFMIWGYKGYVTYKVGGNTKGLSL
                     IAIDSDDLYDANFEDNPVNFRDLDEDWFSMELTNDKGDSTLVEDEFDRLGDYIVGVEI
                     IAHEPE"
     gene            6323..7009
                     /locus_tag="IWL30_001165"
     CDS             6323..7009
                     /locus_tag="IWL30_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9705947.1"
                     /translation="MQIKKATDIEQTKGTYLIYGAPGKGKTSTAKYFPGKTLILDIDR
                     TSKVLRGEKNVDIVYIDNEDTWNDWGNTLADLTTNYVGVYDNVVVDNVSELERCILSS
                     LGAEGKNNGVPSQGDYQYMQFRMVNSLRYMKNLDSNLIWTAWEEIDLWTDSDGSSYNI
                     ALPQINRKIRNNILGLCDVVGRLMVKEDDERGFILQATDSTYAKNQLDNRSGCKQSEL
                     ILDGIVRLPE"
     gene            6984..8333
                     /locus_tag="IWL30_001166"
     CDS             6984..8333
                     /locus_tag="IWL30_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP9705948.1"
                     /translation="MELYDYQNDLVDRARQAYVDGYKAPCIVSPCGSGKSVMVAEIAK
                     RTTRKGNRVLFLVHRRELIDQIKDTFQKMGVDNQLVNFGMVQTVVRHLKTIKKPQLII
                     TDENHHGLAASYRKIYEYYSDVPRLGFTATPIRLNGSGLGDVNDLLIEGVSAKWLIEN
                     HRLAPYEYYAPKLIDTAELKKASTGDFTKKSMDKAVKNTIYGDVLKHYKTLAEGEQAI
                     AYCHSIDASKHTAEIFNDAGYKATHIDAKTPKDERADIIEAFRNHEIKILCNVDLIGE
                     GFDVPDCSTVIMLRPTQSLSLYIQQSMRGMRYRPDKTSIIIDHVGNVNRFGLPDMDRE
                     WSLDTKKKTKTDSDLSIVQCAFCFGAYERPKGDNICPYCGELQPIEERKSELEIDESA
                     ELMKVGETKITLNFENNKYYNMTEDEAESIEDLYAIARAKGFKPGWAYMAAKRKGWL"
     gene            8324..8797
                     /locus_tag="IWL30_001167"
     CDS             8324..8797
                     /locus_tag="IWL30_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9705949.1"
                     /translation="MVVGMGGIFGKKAEDVQGSIINGVTIIGDTGKRDSSGNQIVIAR
                     NKNNEVFESSLSNIKRGVVNGTGEESGYVNLKKANEKYHVEGTYVKALSVGGTKGVNW
                     DKRRNKWKARLYFKGEYVLQKDFNTKEEAIQARLAAEEKYFKPILEKYENKKEND"
     gene            8799..9299
                     /locus_tag="IWL30_001168"
     CDS             8799..9299
                     /locus_tag="IWL30_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010050472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HAP9705950.1"
                     /translation="MTGFNLDFSKAQQGNEIKDGTYEVVVNKAVENATKSGAEFIDID
                     LIVRNDVDQPFQNKHIFAKIWKAKATGKYNEGMIMAIAQALQLEDGKSYNGFDELLAD
                     FVLKTASVRVKTEESNGYKNVNVKSWDKTNTRGVMNHQFKNGDEPSFGPERSRATTVR
                     NDNLPF"
     gene            9314..11626
                     /locus_tag="IWL30_001169"
     CDS             9314..11626
                     /locus_tag="IWL30_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP9705951.1"
                     /translation="MYEKIPKELKNLKQWCVYKLVWDEKRQKHTKIPYNANNGHKAKS
                     NDESTWSDFQTALAAIDHYGMSGLGFFFKPPYFGIDIDNAEGEVERFKSGDIEENIIY
                     EFIESMRSYAEYSQSGTGIHIIARGELPGGRRRKGDVEMYQNGRFFVMTGNAASKYLE
                     ITEPNPKDIKRLYDRYVGDEKIIQFKEENPLMNTVDLPVEEIIKRAESSSQGARFKVF
                     MNGGWEAFYSSQSEADMAFANDLAFWTGRDFEKMDEIFRASAMIRSKYDQKRGSATYG
                     ESLLNKAIADTNSVYNPKRKSDFKIFIKDQEQPKEEKYYSYDDTGNADRFTDIYGTLV
                     KYSYIDKSWYYYDGKVWLQDNTGEVRKMIDTTVDIMGKEPLTIPEGADDETKEALMKA
                     KEKHVKRSRSNAGKNAMMDELKHRLSVLPEEFDKDKTLFNTQSGYLSLHDGLLHEHEI
                     DKMFTRVSNVEYTESVDCPMWEEFINQIFDNDQELIRYIQKCVGYSLTGSTREQCMFI
                     LYGHGSNGKSVFLEIISELMGNYAMTMQAQTIMVKQSQSSANSDIARLKGARLVTSSE
                     PNEGVRLDEGLVKQLTGGDKVTARHLYGKEFEFEPEFKLWLATNHKPIIRGTDDGIWR
                     RLNLIPFTVQIPDHKKDKNLKFKLQTELQGILKWAIDGCLLWQREGLEKPKSVVAASQ
                     DYRNEMDQIGTFIETCCETGPGLKISGGELYKVYREWASDNGEHTFTNTKFGREISKK
                     YSKEKAGGFMVYKGITLKPRKYDNVRELFK"
     gene            11873..12190
                     /locus_tag="IWL30_001170"
     CDS             11873..12190
                     /locus_tag="IWL30_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HAP9705952.1"
                     /translation="MKSEHQIQDEIRIALSKHGCTIFRSNAGQVTTIEGRQFYGMPKG
                     FPDLCGHRNSDGKAIYIEVKNERGKLREDQKRFAEFLNGQPVLYGVARSVEEALKIVE
                     ESS"
     gene            12187..12348
                     /locus_tag="IWL30_001171"
     CDS             12187..12348
                     /locus_tag="IWL30_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP9705953.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            12345..12650
                     /locus_tag="IWL30_001172"
     CDS             12345..12650
                     /locus_tag="IWL30_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705954.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWSEKTK
                     RRTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRAKRENVDSKGRQFKHWEKK"
     gene            12650..12964
                     /locus_tag="IWL30_001173"
     CDS             12650..12964
                     /locus_tag="IWL30_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705955.1"
                     /translation="MEELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EYGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLEDS
                     DE"
     gene            12957..13487
                     /locus_tag="IWL30_001174"
     CDS             12957..13487
                     /locus_tag="IWL30_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP9705956.1"
                     /translation="MNKKVLIDKQALIDELMKIPGVGSNSDALETIKRFPSYEPQKPV
                     VPKFVAELLDYYRQSTDVDLLALLITFHDWYYRKTKDGEHEEAIDWLVDHPEIYMRAW
                     LFGYELEKEPLYCVIINGRYLVKVFSNTNVVILVPADEFSQYVTQKFQLTEKQIKAID
                     ERYWPFAVPVDEVVEG"
     gene            13484..13753
                     /locus_tag="IWL30_001175"
     CDS             13484..13753
                     /locus_tag="IWL30_001175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705957.1"
                     /translation="MSDPYITKLEKLSEDFEKNKNKLTKDKLIELVRLVIRGAITREK
                     AQQNIEIFARSVRDGLEKKVKQNEQQTPQNNKTKKTGTECTKDKV"
     gene            13883..14170
                     /locus_tag="IWL30_001176"
     CDS             13883..14170
                     /locus_tag="IWL30_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705958.1"
                     /translation="MKLKDGFYSSSHGIGGLMLDMPTKNPKTREKPKFKVGDMVRCEA
                     EGFIYPFCGYVEHLYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMEVI"
     gene            14657..15070
                     /locus_tag="IWL30_001177"
     CDS             14657..15070
                     /locus_tag="IWL30_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP9705959.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            16266..16811
                     /locus_tag="IWL30_001178"
     CDS             16266..16811
                     /locus_tag="IWL30_001178"
                     /inference="COORDINATES: protein motif:HMM:NF024252.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9705960.1"
                     /translation="MHMHIGEKIYKLRRLNNETMNDLVKQTGVSKSWLSDIESGKKQR
                     VDFNKICAIAKHYKVSLEYFRDDYLDSDEHEDIIPLTGETRVKSVKSLEQSLKYLNNK
                     QEPNLLEIKLKDTDSVPEVWYKGERLDELPKGLVDVSYHWKTDDFTNDDRGANDITIQ
                     YFSSFNDKYPDKKTIGHKRDM"
     gene            16812..17129
                     /locus_tag="IWL30_001179"
     CDS             16812..17129
                     /locus_tag="IWL30_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP9705961.1"
                     /translation="MKEVRPRDEIDKLYKTKRWRDLRQVVIARDFGMCQECKRRGRNT
                     RGTIIHHIVEAREDLSLFWSVDNLECICVACHNREHPERSGGKKKPKPKSHIVKMYST
                     PER"
     gene            complement(17286..17450)
                     /locus_tag="IWL30_001180"
     CDS             complement(17286..17450)
                     /locus_tag="IWL30_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HAP9705962.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGQEVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            17552..18073
                     /locus_tag="IWL30_001181"
     CDS             17552..18073
                     /locus_tag="IWL30_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAP9705963.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            18045..18500
                     /locus_tag="IWL30_001182"
                     /pseudo
     CDS             18045..18500
                     /locus_tag="IWL30_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            complement(18540..18698)
                     /locus_tag="IWL30_001183"
     CDS             complement(18540..18698)
                     /locus_tag="IWL30_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HAP9705964.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            18791..19552
                     /locus_tag="IWL30_001184"
     CDS             18791..19552
                     /locus_tag="IWL30_001184"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP9705965.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            19739..21091
                     /locus_tag="IWL30_001185"
                     /pseudo
     CDS             19739..21091
                     /locus_tag="IWL30_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            21107..22327
                     /locus_tag="IWL30_001186"
     CDS             21107..22327
                     /locus_tag="IWL30_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP9705966.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDVEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALVKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGSNEGLKVKFASNMDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGNNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTTSSKSVAENG"
     gene            22362..23039
                     /locus_tag="IWL30_001187"
     CDS             22362..23039
                     /locus_tag="IWL30_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP9705967.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGINCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAEAPQEETLLQKKLKA
                     LNGGKNE"
     gene            23036..24229
                     /locus_tag="IWL30_001188"
     CDS             23036..24229
                     /locus_tag="IWL30_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP9705968.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRRFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     ITPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSKWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIVNVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTTIANE
                     TPAKV"
     gene            24244..24570
                     /locus_tag="IWL30_001189"
     CDS             24244..24570
                     /locus_tag="IWL30_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP9705969.1"
                     /translation="MILDPKMDLGEIKNALKIDTDDDDVEVSRAAQAAIAYIRGAIGN
                     DKPSFYKQENETVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKASYL
                     LFVEEE"
     gene            24574..24909
                     /locus_tag="IWL30_001190"
     CDS             24574..24909
                     /locus_tag="IWL30_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP9705970.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVPTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            24906..25301
                     /locus_tag="IWL30_001191"
     CDS             24906..25301
                     /locus_tag="IWL30_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705971.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEELRQNT
                     PYWDGTKSNGKRGSYMQEHAKNHVVTSSIKNGLVEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            25298..25669
                     /locus_tag="IWL30_001192"
     CDS             25298..25669
                     /locus_tag="IWL30_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705972.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EISGNYEDFASNNPLTIQFNVQVDVWVSTMKEVDAFYFALDEVMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            25685..26245
                     /locus_tag="IWL30_001193"
     CDS             25685..26245
                     /locus_tag="IWL30_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP9705973.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGANVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLTKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEGMTLQKFKDLVNNKEGE"
     gene            26370..26681
                     /locus_tag="IWL30_001194"
     CDS             26370..26681
                     /locus_tag="IWL30_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705974.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     K"
     gene            26705..26896
                     /locus_tag="IWL30_001195"
     CDS             26705..26896
                     /locus_tag="IWL30_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705975.1"
                     /translation="MDQIYKDLIEAGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            26896..30752
                     /locus_tag="IWL30_001196"
                     /pseudo
     CDS             26896..30752
                     /locus_tag="IWL30_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
     gene            30753..31625
                     /locus_tag="IWL30_001197"
     CDS             30753..31625
                     /locus_tag="IWL30_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705976.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSESVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            31622..32671
                     /locus_tag="IWL30_001198"
     CDS             31622..32671
                     /locus_tag="IWL30_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705977.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGVDEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            32675..33499
                     /locus_tag="IWL30_001199"
     CDS             32675..33499
                     /locus_tag="IWL30_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705978.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            34057..35289
                     /locus_tag="IWL30_001200"
                     /pseudo
     CDS             34057..35289
                     /locus_tag="IWL30_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331001.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        12183 a         5963 c         7688 g         9535 t
ORIGIN      
        1 ttccttaact tgtcttttgt atataactaa ccattcttca acaagctcat gaaatgttag
       61 atcaggctta tgaagtgttt tttcgtttaa ttttatatca atttttttat ttaactctag
      121 caatgccact ttctgagttt ctcgagattt atttttataa gtaatgctca ctttttttct
      181 ttttctagtt tttggatcaa tatatcgctc attatattta tatacttttt ttccgttttt
      241 atcagtttta gtttcgatcc acattgtttt catctcctat ttgttgctat aataggaata
      301 gataagtaag ccgattatag caggtttatt tttcatcacg tccacaaact ttggcgaggg
      361 aggggcgtgt ttttgttagt tattgtggta ggttatctaa agcgtattgt gcttgctcat
      421 ttgtaaaacc ttcaaatata agttgatcgt acaattgcga atctgacatt gatgcccaat
      481 catcgtaact ttccgcttta gccaaagctt gttcgttgta atcgacatca acattagcca
      541 gcgcataatc gattgcatca cttggatatt cttcaaactc tagttgttca cgcaaacctt
      601 gttcagacat accagcccaa cctagatagc tttcagcttt gctcaatgca tttctgtatt
      661 cacgaggaac actttcttct tgagtaactt cttctatagt agtttcagta gtttcagttt
      721 cttcttcaac gactgtagat tctgctaccg attctgcaga agacacttct gtcacgcttt
      781 ctttagttgt ttcagcgacg gtatttttag catcatcact gcctccttgc gaacctatag
      841 caataaacac cacaacaacc gctaacaacc aaaaccatac ccgtttataa aacggtttct
      901 taactttata cattttccca tcttgaccca taactttttt tgccatttaa atatccctcg
      961 cttcttgtta taatatattt gtgatctcag aaatgaggta tgagtccgtg ttgcagcacg
     1021 ggcttttttc tttataactt ttttagagat tataggcaaa atagtagggc ataaaaatgt
     1081 attattgaat tccgtattta gaaaatccta attgaacttc accggaagtc ttttgctgtg
     1141 ttgtacgcaa tgcttcttct tcagacattc cattctgtac tttccatgca acaggcgaca
     1201 tcccgtattt gttaacaaaa tcagtaagtg ataaagtgtc agcgtcttgc tgagcgcttg
     1261 tttgttggtc ttctggattc tgttgagatg ctgcttgttg ttctttctga tcttgactga
     1321 taatattgcc agcatcatct gtagtcaatc cattttcata aagggccacg ccgaaagctt
     1381 cccactcttt gttggaccaa tttgcacgat cagctggagt tgactgtaaa gtgcgttgtt
     1441 tcatctgttc atatgtttct tcttgaggtg cggtttggat tgtactctga ctggagctta
     1501 taactgttgg gctaggttcc gccgtagctt ggttggagct tgtaactgtt gaactagaat
     1561 ctgttttaga tgtagatttg ctagtagatg aactggtttc agttgtttct tttgttttac
     1621 ttatttttgt ttcttggtta gaagtggtat ctgttgattc agctttttta ttatttgaac
     1681 aagctgaaag tagcagagca gtacttaaca acaacataac gctaactttt ttcattttat
     1741 aattcctctt tctcgttgta atatgtgtgc taacacgggc ttttttatat aagaaaacga
     1801 taagcgcttt ctggaagccc gtaaagattc tttaattcct cgattttttt aggatattga
     1861 tcattatctt ctttataaag agaaacaatg agattagcag caaagcaatt agcttcgctt
     1921 tcagatttgc ttctagatgt tcttgttgat acataatagc tggataagcc acgatgaaaa
     1981 atagcgtgac ctaattcgtg agcgcaaatg tagaatcttt cctcagagtc tcgcagttca
     2041 tcatttaaaa agattatcgc acgatctcta atttcttgaa actgcccctt gggattctcg
     2101 ataaaaggaa cgtattgaat tttaatgccc atcttttcac aaatataaaa aggattagcg
     2161 gactggtatt tccgcttcaa ctcctcgact aaattaatcg tatccatctc cataagctca
     2221 catctttttg cctttttctt tgtcttcttt cacaatatcc cagaaagtcg ctatcaggat
     2281 atcttttacg cgctgtattt gttcgggtgt caatgtttcc ccaccataag acatattaac
     2341 atttgagtct agtagtttat caagttcaac cacttcctct tttgtagccc acttgggaac
     2401 attattattt cccaataaat aatcagttgt gacaccaaaa taatcagcaa ctttctttaa
     2461 gttctcagat tttggcgagg ctttatccca tcttcttatt tgtccattag agatgcccac
     2521 ctgtctttct acttctgcta tagtcacatg cttttcgtct gctaattctt taatcttagt
     2581 aactaaactc attattatca acctttcaaa gctgaaaaga aaataaatag cttaaaagtt
     2641 atatttttgg ttgacaatta gcttttaagc tagtatattt aattcgtaag ctaaattgtt
     2701 agctaaataa gagcaacaaa aaactctact aatttaaaac attctctcgg tcgccaaact
     2761 tagaaatgtt attttagagg ctttttataa gtcttattta actatgtatt cataatagct
     2821 taaaagctaa taggtgtcaa cgatttagct aattttttag cttacaaatt atttgtttag
     2881 aaaggagcta tttttatgtc tgagaattta gacttaaaaa ttcgagcgga gatgagaaaa
     2941 agaagaatga ctttcaaaga actagctgcg cttatcggta tttcaggagc ttatttatca
     3001 gatattctaa acggcaatcg tgatggaaag aaagcacaac agcatatcga aacagtgaaa
     3061 aaaatattgg acatccgata gggggggtag gcgatgacaa aactaaaaaa acaagatttt
     3121 gtaaaaaaat ataattattc tccatctact tatcaacgtc gaatgtcgga actaaaaaat
     3181 acagcaattt tctcagcggc gtatgaacgg gtcacaggac aagaagtttg gatcaataca
     3241 gaattatacg ataaattttt gtctttcaaa tcctataaca ggttacgcac aagaaaggta
     3301 acacctaaag aatttatcga gaagcattta gttgatttat aaaaaacaga acattttgag
     3361 aggtgaaggt taatgggtaa attcaacaga gcattagtat tcagcgcacc gctaatcgtc
     3421 tatgctttag ggctttgggg aagtagacaa gcgttgatag gaacgattgt gtacatggtt
     3481 tggattttta tagggctgga tgaagctgag tacaaaacaa aaaagccagt cgggagggac
     3541 tgactaatga gtaaggacga tattgtattt ctgttaatta tgttttgtgg gattggatca
     3601 ataataatta atttgtattt gattattaaa gaaaaatttt tcaattaaaa tgagtctttt
     3661 agctttaact taaatttttt ttgataatta gctgttctta tgaccattac aagtttttca
     3721 ccaggtaata tatctttcat atctaaaact tctgaaaaag cgactgagta gccaggagag
     3781 attgcttgtg ccaacggtat aggagataaa aggctacttt tatgaatgct tcccatatca
     3841 aaaccattgg cgtgataatg tgaacaatta attttcttac cgttgtaaag atacgtacat
     3901 tcgataattg agtttggcat ttttgatggg tttgcaatta cgccagtaag aatcattcga
     3961 ttacttctat catgcttaac taaaaaatag gagagcgtaa tttttctttt atctttaaat
     4021 aagttataga gggataaaaa aaatcctgaa atagaaatga ttaaagtaat agcgacttgg
     4081 taatcgctca aaatttttat caagtaattc accacctttt tactcaaatt atatcaaaaa
     4141 ggagagaaga aataatgcaa gaattagtaa ttttgaaaaa taaaaaaaga cacgaacgcc
     4201 ggcaagcatg atcgtgtcag ggaaataaac tatctttcta tattttacca cagaaaggaa
     4261 tgaaccgtaa atgctaattg caacggatac actagaccgc atctttttaa aagacgaata
     4321 caaactgcgc aatatagatg cgtcaggaat tttagtattt gatctttatg acaatggaaa
     4381 aattggtatc tatcaagcaa gtgatattga agaaacaaac cttgctttcg aacaaattga
     4441 tgattctgtg gaattggatt tagatgaggc aatcctagct tttgaacaaa ttgcaaaatt
     4501 attaaaggag gcacaaaaga atggcaactc tttaccaact cagcgagtca tatatcaaag
     4561 tcctggaact ggcagaacaa ttggatgaag aaattcttcg cgatactctc gattcgatta
     4621 atgaagcgat cgagtataag gcagaaaact tagcaaaaat agttaaagaa gtagaaggga
     4681 aagctgagtt aatcgatagt gaaattaaac gtctgcagga acgtaagaca tcgcttttga
     4741 acaatgctaa gagtatcaag cactatttac aagaggaaat ggaaaagact ggtaaaacga
     4801 agattaaagg tgaattattc aacattggaa ttcaaaataa tccagtatcg gtcaatgtaa
     4861 tcaatgagaa cttaattcca aaaggattct ttacccctgt gcctcccaaa ttggataaaa
     4921 agcaattgaa ggaggaactg aagcacggag atattcctgg tgctgaactc gtccaaacaa
     4981 aaggtttgag gataagatga tgaaggagga gaaataatgg aacacgaaaa cgtaaaagac
     5041 gctttgaaag ctgctatcga aatcgcggaa gctaaaggaa taaaggttga cggcaaacca
     5101 gcaactgtcc atgacattca aaatttgacg aaagaacatc tttattttat cgccgacttg
     5161 cttggtttgt cggaattgta tttagacaaa taaccccaca cggaaggagt ggctagggtg
     5221 gttgtattac aagtaatagt ctgcgtaaca ttcatttggt cgtgcatacc ggtttgtata
     5281 tatatcatga agaagcttcg ccctgagtct tacaaattat acgcaattta cagcataaca
     5341 gtagctcttg catttacatt gtttgcaatt tctaggcttc cgttttaatc gtgggaatga
     5401 atttttaatg aactaaatta tctataaagc aaaaaaagga gaaacaacat tgaaaaacaa
     5461 actagcaaaa acaacagcaa tcatcggact agcactaggt agcggcgtta tcggctacgc
     5521 agcaagcaat gcgtttcaag atttggacac gatcaaagca aatttcaaca cagtcctaca
     5581 atacggtcaa acgaaatcgc aacgtgtgtc agaacttgaa tcacagctat ccaacaacac
     5641 tcgcacacag gagcagctga aagccgaaat tgagcaaatc aaatcggaca agcagaagga
     5701 aattgaagcc aagcaacgag aagtcgaaca aaagcaacag gagatcgcta caaagcaaca
     5761 ggaagccgat agcttgcgcc aacaactatc cacagtgcaa aacgacaagg agcagttaga
     5821 acagcgcgtg agcgagttac ggcagtatac ggatcaaaag gtaggggagt tggggaaatg
     5881 aaagagttga ctatttatga cccaaatatt tcatgggcta aacacacaat taaagtttct
     5941 tttatgattt ggggttacaa aggatatgtt acttacaaag tgggcggtaa cactaagggt
     6001 ttatcattaa tagctattga ttcagatgat ctatatgatg ctaatttcga ggataaccca
     6061 gttaatttcc gtgaccttga tgaagattgg ttctctatgg aacttactaa cgataaaggc
     6121 gacagtacac ttgtagaaga tgaattcgac agactaggtg attatattgt cggtgttgaa
     6181 attattgcgc acgaaccaga gtaggagagt gaatacaaaa tgattaaaag aatcactata
     6241 tcagttgaag aagatagtgg atcaacaatg cagcataccg acaatcgaaa aactttttta
     6301 accaatagaa aggagaattt tgatgcagat taagaaggcg acggatatcg aacaaaccaa
     6361 gggaacatat ttgatctacg gtgctccagg aaaaggtaaa acttcaacgg ctaaatattt
     6421 tccagggaaa acactgatcc ttgatattga tcggacttca aaagtcctta gaggtgagaa
     6481 aaatgtcgat atcgtatata tcgacaacga agatacctgg aacgactggg gcaacacgtt
     6541 ggctgattta actaccaatt atgtaggtgt ttatgacaac gtggttgtcg ataatgtcag
     6601 cgaattggaa cggtgtattc tgtccagttt aggcgcagaa ggaaaaaaca acggcgttcc
     6661 ttcgcaaggc gattatcaat atatgcaatt tagaatggtg aacagcctac gctatatgaa
     6721 aaatttagac agcaacctta tctggaccgc atgggaagaa atcgatttgt ggacggattc
     6781 ggatggatct tcttacaaca tcgctttgcc gcagatcaat cgaaagattc gaaacaatat
     6841 tttgggatta tgtgacgttg ttggtcgatt gatggtaaag gaagacgatg aacgcggatt
     6901 tattttacaa gccacagatt caacctatgc caaaaatcaa ttggataata gatctggctg
     6961 taagcaaagt gagctgattt tagatggaat tgtacgacta ccagaatgat ttagtcgatc
     7021 gtgcaagaca agcctatgtt gatggttaca aggcaccatg tatcgtatca ccgtgcggtt
     7081 ctggaaaatc agtcatggtt gcagagatcg ctaaacgaac cacacgaaaa ggcaacagag
     7141 tgttgttctt ggtccatcgt cgtgaattga ttgatcagat caaagatacg tttcaaaaga
     7201 tgggcgtaga taatcagctt gtaaatttcg gcatggttca aacagtcgta cgacatctta
     7261 aaaccatcaa gaaaccacaa ctgatcatca ctgacgaaaa ccatcacggg ttagcagcgt
     7321 cctacagaaa aatctatgag tattattcgg acgttccgcg attaggtttt accgcgacac
     7381 ccatccggtt aaacggtagt gggttggggg atgtgaacga cttgctaatt gaaggagtat
     7441 cggctaaatg gttgattgag aatcatagat tagctcctta tgaatattat gcacctaaat
     7501 tgatcgatac agccgaatta aagaaagctt cgactggaga ttttacaaag aaatcaatgg
     7561 ataaggcagt gaagaatact atttatggcg acgtgttgaa acattataaa acgttagcag
     7621 aaggtgaaca ggcaatagca tactgtcaca gtatcgatgc aagtaaacac actgctgaaa
     7681 tattcaacga tgctggttat aaagcaacac atatcgatgc aaaaactcca aaagatgaac
     7741 gtgcagatat tatcgaagcg ttcagaaatc atgaaattaa aatcctatgc aatgttgatt
     7801 tgatcggaga agggtttgat gttcctgatt gttcgactgt aattatgttg agacctactc
     7861 aatcgttatc attgtacatt cagcaatcga tgagggggat gcgttaccga ccagataaaa
     7921 cgtcaatcat cattgaccat gtgggtaatg tgaaccgttt tggattgcct gatatggatc
     7981 gtgaatggag tttagataca aaaaagaaaa caaaaactga tagtgattta tctatcgttc
     8041 aatgcgcgtt ttgttttggt gcttacgaac ggccaaaagg cgataatatt tgtccgtatt
     8101 gcggagagtt gcagccgatt gaagaacgaa agtcagagtt agaaatcgat gaatcggctg
     8161 aattaatgaa agtcggagaa acgaaaatta ctttgaattt tgagaacaat aaatattaca
     8221 acatgacgga agatgaagca gaaagtatag aggacttata tgccatagca agagctaaag
     8281 gatttaaacc tggttgggct tatatggctg ctaagaggaa ggggtggttg taggaatggg
     8341 cggtattttt ggtaaaaaag cagaagatgt tcaaggttca ataataaatg gcgtaacaat
     8401 aataggtgat actggaaaaa gagatagttc aggcaatcaa attgtaatcg ctagaaataa
     8461 aaataacgaa gtttttgaat cttctctatc taatataaaa agaggtgttg ttaacgggac
     8521 aggcgaagag agtggctacg ttaatttaaa aaaagctaat gaaaaatacc atgttgaagg
     8581 aacttacgtt aaggctttgt ctgtcggtgg aacaaaaggc gttaattggg ataaacgaag
     8641 aaataaatgg aaagcacgat tatatttcaa aggagaatat gtcttacaaa aggatttcaa
     8701 caccaaagaa gaagcaatcc aagcacgact tgcagcagaa gaaaaatatt tcaaacctat
     8761 cttagaaaaa tatgaaaata aaaaggagaa tgattaacat gacaggattt aacttagact
     8821 tttcaaaagc gcaacaaggg aacgaaatca aagacggtac gtacgaagtg gtcgtaaaca
     8881 aagctgtaga aaatgcaact aaatcaggcg cagaatttat cgatatcgat ttgatcgttc
     8941 gtaacgatgt ggatcaaccg ttccaaaata aacacatttt cgcaaaaatc tggaaagcca
     9001 aagctaccgg caaatataac gaaggtatga tcatggcaat tgcacaagcg ctacagttgg
     9061 aagacggaaa atcatataac gggtttgatg aattgctggc ggactttgtt ttgaaaaccg
     9121 cctcagtacg cgtgaaaact gaagagtcaa atggatataa aaacgtcaat gtgaagtctt
     9181 gggataaaac gaacacacgg ggcgtaatga atcaccaatt caaaaatggt gatgaaccat
     9241 ccttcggacc ggaacgttca agagcgacaa cggttagaaa tgacaatctt ccattttaat
     9301 tgaggtgtaa tcaatgtatg aaaaaattcc aaaagagctt aaaaatctca aacaatggtg
     9361 tgtttataag ctagtttggg atgaaaagcg gcagaagcac acgaagatcc cttataacgc
     9421 caacaacggg cataaagcca aatccaatga cgagagtacc tggtctgatt ttcagaccgc
     9481 tctcgctgct attgatcact acggaatgag tggactgggg ttctttttca aaccacctta
     9541 tttcggcatt gatatcgata atgctgaagg tgaagttgag cgatttaaat caggtgatat
     9601 agaagaaaat attatctatg aatttatcga aagcatgaga tcctatgctg agtattctca
     9661 atcaggtact ggcatccaca ttattgctcg cggagaattg cccggcggaa gacgcaggaa
     9721 aggtgacgta gaaatgtacc agaacggccg attcttcgtt atgactggaa atgccgcatc
     9781 aaaatattta gaaataactg agccgaatcc aaaggatatc aaacgcctat atgatcgcta
     9841 cgttggagat gaaaaaatca tccaattcaa agaagaaaat ccgctgatga ataccgttga
     9901 tttaccggtt gaagaaatta tcaaacgtgc tgaatcgtct tcccaagggg caagattcaa
     9961 agtctttatg aacggcggtt gggaagcttt ttattcctcg caatccgaag cggatatggc
    10021 gttcgccaac gacttggctt tttggaccgg tagagacttt gaaaaaatgg atgagatttt
    10081 cagagcgtct gcaatgatcc gtagcaaata tgaccaaaaa aggggatcgg caacttacgg
    10141 agaatctcta ctaaataagg caattgctga tacaaattcc gtctacaacc ccaaacggaa
    10201 atctgatttt aagattttta tcaaagacca ggaacagcca aaagaggaaa aatactattc
    10261 gtacgacgac accggcaacg cggatcggtt tactgatatc tacggaacgt tagtcaaata
    10321 ttcgtacatt gataaatctt ggtattacta cgatgggaaa gtttggctac aggacaatac
    10381 cggcgaagtt cggaaaatga tcgatacgac agtagacatc atgggaaaag aaccgttaac
    10441 gatccctgaa ggcgcagacg atgaaaccaa agaagctttg atgaaagcga aagaaaaaca
    10501 cgtcaagcgt tctcgcagca atgccggaaa aaacgctatg atggacgaat tgaaacatcg
    10561 actatccgta ttgccggaag agttcgacaa agataaaacg ctgttcaaca cacaaagcgg
    10621 ctatctgtct ttgcatgacg gtcttctaca cgagcatgag atagacaaaa tgtttacacg
    10681 tgtttcaaat gtcgagtaca cagaatcggt cgattgtcct atgtgggaag aatttattaa
    10741 tcagattttt gacaatgatc aagaattaat cagatatatc cagaaatgtg tcggatattc
    10801 cctgaccgga tctaccagag agcaatgtat gttcatcctc tacggtcatg ggtcaaatgg
    10861 aaagtcggta tttttggaaa ttatttctga attgatggga aactatgcga tgactatgca
    10921 ggcacaaacg atcatggtca agcaaagcca atcatcagca aacagtgata ttgcacgatt
    10981 gaaaggtgcg cggttggtca cttcgtcaga accgaatgaa ggcgtccgac tggacgaagg
    11041 tctggtcaaa caattgactg gtggtgataa agtcacggct cggcaccttt atggaaaaga
    11101 attcgagttc gaacctgaat tcaagttgtg gctggcaacg aaccacaagc ccatcatccg
    11161 tgggaccgat gacggcatct ggcgacggtt aaatttaatt ccattcactg tccaaatccc
    11221 ggatcacaaa aaggataaaa atctgaaatt taaattgcag acggagttgc aagggatcct
    11281 taaatgggca atcgatggtt gcctgttgtg gcagagagag ggcttggaga aaccaaagtc
    11341 ggttgttgca gcgagtcagg attatcgaaa cgaaatggat caaataggta catttatcga
    11401 aacgtgttgc gaaacaggtc caggactaaa aatatcaggc ggagaattat acaaagttta
    11461 tcgtgaatgg gcatcagata atggcgagca tactttcact aacacgaagt ttggcagaga
    11521 gatttcaaag aaatatagca aagaaaaagc tggtggattt atggtttata aaggaatcac
    11581 attaaaacca agaaaatacg ataacgttag agaacttttt aaataaatct ggagggttct
    11641 ggagggttga gaacgaaacc ctccaaccct tagaaacgtt gatatatcag tatttatatt
    11701 atatttttct ttctttctgg agggttttaa aaaaataata aagtattaaa gtaaaaaata
    11761 aaaagtaaat tatataaaaa agaataggtt tttgggtctg accctccaaa ctctccacca
    11821 aaatcttcct aaatgctttg ataccaacga ttaaacagtc tggagggtta cattgaaatc
    11881 agaacatcaa atacaagacg agatcagaat tgccttatca aaacatggtt gcacgatttt
    11941 tcgatccaat gcgggacaag tgacaacgat cgaagggcgg caattttacg ggatgccaaa
    12001 agggttccca gatctatgtg gacatcgtaa ttctgatggg aaagcaattt atatagaagt
    12061 aaaaaatgaa cgcggtaagt tgcgtgaaga tcaaaaaagg tttgctgaat ttttaaacgg
    12121 tcaacctgta ctatacggtg ttgcacgatc agtggaagaa gcattgaaaa ttgtggagga
    12181 atcatcatga caacagaaga agtgattcaa atgcgtattc gaagccttca gcgtgagatt
    12241 gacgatctgg aacggacaaa ggcagtgatg gtcaatgaaa cggcgaggaa ggcaatcgat
    12301 ttgcacatag agaatttaag aagggaaatc catcgattgg aggaatgagc gtggataaga
    12361 aagcagcaat gaaacgaatc atcgaactga cacattctga gaattggcaa gaagacaaag
    12421 aaatagttgc agaagtccaa aagctcggta aatcaatgtg gagtgaaaaa accaaacgga
    12481 gaacaccgag aaaaattgca atctggcatg gtgaccgaat tctagtaaca ggtactgctg
    12541 aacagttatc tgaaattact ggattaagca aaaacattat ctgggataga gctaaacgcg
    12601 agaacgttga ttcgaaaggt cgtcaattta aacattggga gaagaaataa tggaggaact
    12661 aatcacaaaa gtagagcagt gggcaaaaga taagggattg gatcaagctg attccagcaa
    12721 gcaaatgtta aaaacgatcg aagagattgg ggaagttgcc gcttctctag ctagaaaaga
    12781 tgaatatggt ttaagagacg gaattggaga cgtagtagta accttgatta ttttagctat
    12841 gcaaaatgat atggatttgt acgagtgtct gaaccaagca tacaacgaaa tcaaaggacg
    12901 cacaggaaaa atggtagatg gtgtattcgt gaagtcgagt gatttggagg acagcgatga
    12961 ataaaaaagt attaattgat aaacaagcat tgattgatga attaatgaaa atacctggtg
    13021 tgggatctaa tagtgacgct ttagaaacga ttaaacgttt cccatcgtat gaaccgcaga
    13081 agccagttgt gcctaagttt gtggcggaac tactcgacta ttatcgtcag tcaacagatg
    13141 ttgatttatt agctttattg ataactttcc atgattggta ttatcgcaaa actaaagacg
    13201 gtgagcatga agaagcaatt gattggctag ttgatcatcc tgaaatttat atgcgtgcat
    13261 ggctgtttgg ctacgagctc gagaaagagc cgttgtattg cgtaataatc aatggtagat
    13321 atcttgtcaa agtattcagc aatacaaacg ttgtcatatt agttccagca gatgaatttt
    13381 cacagtacgt tactcaaaaa tttcaattaa ctgaaaaaca aatcaaagca attgatgaaa
    13441 gatactggcc gtttgctgtg ccagtggatg aggtggtaga aggatgagcg acccatacat
    13501 tactaaatta gaaaagttgt ccgaagattt tgagaagaat aaaaataaac taactaaaga
    13561 taaacttata gaactggtta ggttggttat cagaggagct attacaagag aaaaggcgca
    13621 gcaaaacata gaaatttttg ctcgtagtgt ccgagatgga ttagagaaga aagttaaaca
    13681 aaatgaacaa cagacaccgc agaataacaa aactaagaaa acgggaactg aatgtactaa
    13741 agacaaagtt tgaaaaagaa tatggaattt cagcagaaga aacatataaa gtggcaagtc
    13801 agtgtgttgc tgatgcgagc ggcgctattc gtaagtttgg aatttcgata ttaagtgatg
    13861 atcgtaaatg ggaggaaaaa gaatgaaact aaaagacgga ttttactcca gcagtcatgg
    13921 tatcggcggt ttaatgctag atatgccgac aaagaaccct aaaacacgtg agaaaccaaa
    13981 attcaaagtc ggtgacatgg ttcgctgtga agcagaagga ttcatctatc cattttgtgg
    14041 atatgtagaa catctctata atcactcagc aatcattcgt attgaaaaca cgatggaatg
    14101 tgacaagtgg ttagcgaaaa gcaaagagaa tttagcagta gcgagattgg tggatatgga
    14161 ggttatataa acagcgaaaa aatgtacttc taagagaaaa atttattttt tataaaaata
    14221 ggaaagtatc attatgaaaa agaccacaga gataaataaa cctaccacat aaaaaatgaa
    14281 tatttcttgc gattcaatat aggtatattt aacaatttgt acaataaaaa atactatagg
    14341 taaaaaagat aacatgagga gcttatttgt tctagagtgt ttgacaaagt aacaaaatgc
    14401 gacgatacca atacaaacgg gaatccaaaa taacagcatg ctccacatag tcactcaacc
    14461 atctcctata cgctttatta tcaatagata atatcatttt tcgtaataaa gataaaaaaa
    14521 taagtaaggc ttctcattaa aatataaaaa agacagccga ccactgactg cctatataag
    14581 agtattgaaa taaaaagctg ctgatataat aaattccaca agtttattat atcacataaa
    14641 ggagcggttt gacttgatgc aattgttacg agaggtagat ttcaaacaga caagatgtaa
    14701 tgcgagagat gtgctgaaga actttcggcg tttggagcgg atggcaggtc gctctttgat
    14761 agatattaag tcgccgatta ttacggatat gccgaaggcg ccgaagcacg gcaataaggc
    14821 agaagacgca atcattcaga tgatggatat agaagcggag agagacgcga ttctagcagc
    14881 cttgatggct cttagtctga ttagtcgtca gatactctac tatagctttt gtgtgccaga
    14941 tagcttctca aactacagaa ttagccgtga agtgggttat tcagaaagaa gtatacaacg
    15001 gatgaagtcg gaagctctaa tagagtttgc agaagcatat aaacacggaa gaataattgc
    15061 ttataaataa tttggcggct ttttggcgga atgatggcgg tttttagcta tttaccagtg
    15121 atattatggt agtgtcgaaa gattagtgat aggtctaaga caaaataata ataaaaggaa
    15181 catcgtttta ttattgtttc acaattaagc ttcgatagac agcaacggaa atattaagaa
    15241 taaggatgtg aattttaact ccttctaaat tgttcttatt atctatcatc cgttgctgtc
    15301 tattgttttt ttaattattc acacgataac taaaggtgag cgaagagaaa tgattccatt
    15361 aataatttca atttttgcgc tttgtcttaa tgtctatatg attggattta aaaatgggca
    15421 aaataaaaga tagtagtaac taagaaagaa atttttatat tgtcactgtg gcggaaaggg
    15481 tagacgctaa gcatgtgtgc taggtcaatg cttcggcaac tatgcaatgt tcgattcatt
    15541 gccagtgact ttggcaaccg aggcatcggc ggtttaaaaa tataggggtg cgcaatttcg
    15601 tacgcgtttt gtgcatcgtg taggttgcta ttacatatta gatcactcgt tgagtggtct
    15661 ttttatttta cataaaggag gtaacaacga tgtatagacc acaatactta gaacagaagt
    15721 atgaagtaat cactgtgcaa aacggtaacg gtgagatagt acgaaagtat agaagaccaa
    15781 taaagagcga tacatataaa cgaaaggaaa acaatgaagt tatttctttt cgcagaagga
    15841 gaaaagccaa atgagaaact actggtatgt atcgctaact aatgaatatc ctcgaaccat
    15901 tgatgattgt tcagtgcgtg ttgtgcgttc tgtacaaatc aaagggaagt actctattgt
    15961 cgaaatgcta agagaagcta caccaaacga agtggataaa tgcaagctga tatattgcgg
    16021 tcatggctat tggaaagacg agtatatcca acaaaacatt gagaggtgga tagatagatg
    16081 agttacctcg aacatttgaa acgttgctac atgcattcta agaataaact tcctgacagc
    16141 tacactgtag atgatgttgc tatacatgta ttgaagactg agagccatag cagtcctgat
    16201 ggtagcagta aggagcagac gctcgcgtgg ttcaagttct ttaagtggat taaggaggaa
    16261 gagtaatgca catgcacata ggagaaaaga tatataaatt gagacggctt aataacgaaa
    16321 caatgaacga cttagttaaa caaactggag tgtcaaaatc atggttatct gatattgagt
    16381 ctggaaaaaa gcaacgcgtt gattttaata agatatgtgc aatagccaaa cattataaag
    16441 ttagcttaga atactttcgg gatgattatt tggatagtga tgaacacgag gatattattc
    16501 ctttgacagg cgaaacaaga gttaaatcag tgaaatcgct tgaacaaagc ctaaagtatc
    16561 tgaataacaa acaagaacct aaccttctag agatcaaact aaaagatact gactcagtac
    16621 cagaggtttg gtacaagggt gagaggttgg atgaattgcc taaaggatta gtagatgtct
    16681 cgtatcattg gaagactgat gattttacta atgatgatag aggagcgaac gacatcacga
    16741 ttcaatactt ttctagcttt aatgataagt atccagataa gaaaacaatc ggacacaaga
    16801 gagatatgta aatgaaagaa gttagaccta gagacgaaat agacaaacta tacaagacca
    16861 aacgatggcg agacctaagg caagtagtaa tagctaggga cttcggcatg tgccaagagt
    16921 gcaagcgtcg agggcggaac acaaggggca cgatcatcca tcacatagtc gaggcgaggg
    16981 aagacctgtc actgttctgg tccgtagata accttgaatg tatctgtgta gcttgtcaca
    17041 acagagagca tccagagaga tcaggcggga agaagaaacc aaaacctaaa tcacatatcg
    17101 ttaaaatgta ttcaactcct gaaagataag tttgcagcga aatgaaggta gcccccctac
    17161 tctaaaagat taaagagtaa ggcttgagaa gaacggtgct gtccttcctt cgtaaaaaga
    17221 ccgcttttca agttttttgg agaaaaagga aaaagccgac caatttaagc cggctttgga
    17281 cgaagctatt tcttagtcca tttgtttcct ttttgagaag tagggggtaa tcggtcgcct
    17341 ggatcaattt ttacttcttg tccgccttgg acatttccac cacgaggacc cacttctttg
    17401 taggttcctt ttggtttgtt gtcttcgccg ggtttataga gttctcccat aggaatccct
    17461 ccttaacaaa tttcggcaca gcactgccga taacctaatt ataaggattg tgataacgat
    17521 ttcaatctat cttttgaaag aaggtgatat tatgccgcaa ccagcgaaga gtgcaaaatt
    17581 acaattatta aacggaaacc caaataagaa gaataccgaa gaactccgca agcgagcggc
    17641 cgcagaagac aaattaaaaa tggctactga caaaatcaaa ccgccgttat ggctagattc
    17701 gctaggaaag gatacctttg agtttatcgc cgatgaattg ctgtctgtgg atttaatcag
    17761 taatccggac gtccatacaa tggctctata ctccaattgg tattcgcaat acgtttcttt
    17821 agaaaaacag cttcgaaaac tacaacgaga gtacaagttg aactatgcgc ttgcgaaaaa
    17881 ggaggcagag gcgagaggtg agccgtttaa tgaacctaat gaattaattg gtaatccgct
    17941 ctctcggcag atggatacag cgtctcggaa tctccgttct tttggcgctg atttaggact
    18001 atcaccagca gccagagcta agttagctat taagatggct gatgatggtg gtgatgacga
    18061 tgacgacttc taatattttg gatatgtctt acacagaacg tgtggactat tggcaaagct
    18121 atcttgagga gcaagcttct tggggtggct ttttaaaatg tccatatccg gaattgttaa
    18181 ctacttggta tgcggaacga ttaatcgatg gaagcatacc agccagcaaa gaaaatattc
    18241 aagctgctaa acggcatatg cgtgatttgc agcgccaagg aacagatgat tttccttgga
    18301 tctttgacga agaaaaaggt caccggccta ttagatatat cgaaaaaaaa tgtaaaccaa
    18361 ctgaaggcga ctttggttcg tttgttttgc aaccttggca gcatttcata attggatcca
    18421 tgtacggatg ggtacatcgt gatacaggag agcgtcgctt ccgcgaggct cttatttttg
    18481 ttggacgtaa aaacggtaag ttttgccgtt taaaaatcgg gcaaaatcgg tgaaaacctt
    18541 tatttttggc tttcttttag taataattcg atagctttat caagcaattt agacatagga
    18601 accatagttt cttccgacat tttttttaat ccttcgtata aatctttatc aattgcgtta
    18661 gaaattctaa ttctgttttt tagtccgtat ttgtccatgt gtgacactcc tttttatttt
    18721 actataacat ccgattccac cgcttgcaac taccatcaat tgatggtagt atataaaaga
    18781 ggtgagtaga ttgaaaaaat acattattta caaacactta aataaaataa atggcaaaat
    18841 ttatatcggt gtcacaaatg atattggcag aaggtggagg agcggtggga ttgaatataa
    18901 accaccaaag aacgaaaccc aacaccacag aagtttttgg aatgcaatac aaaaatacgg
    18961 gtgggaaaat tttgatcatt tgattattga agaagactta acgatgaaag aagcttttga
    19021 gaaagaaaag ttttatattg aactttatga ctcaactaat aagaaaaaag gttataacat
    19081 cgcaaaaggc ggaaatggcg gaataatata taaagttcac cccaaaggaa tgctagggaa
    19141 aaaacaatct aaagaatttt cttcaaatca cagtaaatgg gcaaagaacc ataagaataa
    19201 ttgtatgaca aacggtgatg ttgtatgggg tgttactcac gaacacccaa aaggcatgtt
    19261 gggaaagcac cacagcaaag aatcaattat gaaaaagaaa gcatatagtg gtgaaaatgc
    19321 tgtaacctca aaaccaattg tcgcaattga acgggatgga actaaaagag aatttcacag
    19381 cgctaagctt tgcatggctt actattcgat tagtacatgt gttttttacc gacttttaaa
    19441 agatggcgcc ccttatgtga taaatcctaa agcgaactac agaaataaag aaaaaatatt
    19501 agctatcgaa ggaattatgt tcaaacataa agaagatacc gaggtaagtt agtagattgc
    19561 gaaaggttac taactaccgt aacgcgtagg agttgaataa atataatgct cccaagagtg
    19621 tccgacagca gataatttgc tgaaaatgta cgctaaactg ggccagaact gactgaccga
    19681 tgaaaatgag ggtgacctcc agagtgcgag ataaaaagct cgcagataat aacaatcgaa
    19741 aacatcacta atttcaggcc tttccacata catggtcgct tatgatgatg aacaaggcgc
    19801 caacgtttac gtattggcaa atgctcgtga tcaagcaagc ttgttgtttg ataaggccgc
    19861 agaaatggtc aaacaatcgc cggcgctctt taagaaattt ggtaagccta aacgatcaag
    19921 tattaattat gctcccgcct tttctaaaat ggaaccacgc gcctcagata gccggaaatt
    19981 ggatgggcta aacactcact ttggtatttt tgacgagatc cacgagttta cgaattacaa
    20041 tctgatcaac gttatcaaga aatcaagagg aaccagaaaa cagcctctga tagtttatat
    20101 cacaactgct ggatatgtat tagatggtcc gttgatgtct tattttgagc aaggtgtgga
    20161 ctgtttggaa catttggaag atgacatcga tgaacggact ttctattatc tggcaaaact
    20221 tgacagtgcg gaagaggctg atgacccaag attatggatc aaagccaatc cgaatatttg
    20281 tctaatgaat tttgttggca tgctagatga ctatgttaag gataaaaaag atccaaaaga
    20341 atatgctgac tggattacca agcaatttaa cttgttttcc gatatcgatg agctgtcatt
    20401 tgtcgatatg cctaccatta aacgaaacaa taaaaccatc gatattgaaa cgctcaaagg
    20461 taagaagtgt gtcggtggtt ttgacttgtc cgaaacggaa gactttaccg cagccgtttt
    20521 agaatttccg cttgaaacag gcgaggtatt cattttgcaa cacacatgga tcccacaagc
    20581 tagatttgat cgagataaca atcaagagcg tatcaaagcg tgggagaagg tgggagatct
    20641 aacgattatt cctggtgatt acgtcaatta tgaatacgtc ttaaattggt ttgtagaaaa
    20701 ttcgaaaatc tatgacattg taaaaatcaa ttatgacaag gccaaggcgc tacgattaaa
    20761 taaagaacta gaaaatgcag gatttgaaac caacgagatt cggcaagggt ttctatcatt
    20821 aggtgggcca atgcaaaact tcaaggaaat gctattggac ggtaaggtga ttttcaacaa
    20881 ttccaagctt taccgatggt atctatccaa cgtcaagctg gtgatggatc gcaactcaaa
    20941 ctggatgccg tctaagcagt ccaagagtag aaaaatagat ggttttgcag caagtttgaa
    21001 cagccacgcc gaagtgttga atatgttggt taatcctgtc ggaaccggga aagtaaccta
    21061 ttactcgatt tccgatttaa tgaatatgta agaaaggtgt ggaggaatga gtattttaga
    21121 tcgtttgcgt tcttttggcc gagcgaagcc gaaagcgagc aaacaagagt attttttgaa
    21181 tgacccggga ttgataccgt atttagtcgg aaaagatgaa atatcagaag ggattttttc
    21241 cgtaattagc cgtgtatcga acgtttttgc gtctctccct ctcaaaatga tagatgtgga
    21301 gtttggccaa ccggacgact gtcctgcata caacttgttg agcgaaggcc ctcgatattt
    21361 tacaaagttt gattttttcc gggacgtgga agttttgaga aactaccaag ggaatgcgta
    21421 tgtgcagatt ttccgaaata tcaatggaga agtagcagat atggcgttag taaaacctgg
    21481 tgcttgccat ccagtgattg atatggatag cggggagctt tactaccaag taactgcgac
    21541 tgacaaaggc agttacaagc aagttatcta tgtacattac atggaaatgc ttcactttaa
    21601 acaaccgagg tttggcggct tggaaggtac agaccccaca aaagtattaa cgaataccct
    21661 cggatatgat cgagaagtcc gaaaaatctc tttaagtcag cttaaaggaa gtaatgaagg
    21721 gctaaaagtt aagtttgcta gcaatatgga tgaagaagct aaaaaagcta cagttaaaaa
    21781 cattgctgat ttttatcgac aaaacggtgg actacttgtg gaagaaaacg gtgtagaaat
    21841 cgaacgttta caacgagagc tggtagacag caagctttta gatactgata aagtatctcg
    21901 ctccagaatc gctatggtct acaatgtgcc ggagcatttc atcgggaata accagtcgag
    21961 ttactcctct caggaacagc tcaatatgga atttttgacg tacaatctag taccgaccgt
    22021 taatcaatat gaagcggaac taaataagaa aacactatcg agaaccgaaa aagcaaaagg
    22081 ttatcgatat aagtttaatg tcgcaagttt gctaagagct gacactcagg caaggggaca
    22141 attctatcaa atcatgcgac gaggcggagc atactcagcg aatgatgtcc gaagatttga
    22201 ggacttgcaa ccgataaata aaaccggtat ggatgattac catatttccg gagacctata
    22261 tccaatcgat atggatccaa cattaagaaa aacaacctcg tctaaaagcg tagccgaaaa
    22321 cggttaggtt tttttagttt gcaccgaagg gaggtggaaa aatgaaaaaa gtgacgttaa
    22381 gcggcgatgt cgtggataac gataccgcgt ggctttatga ctggtttggg atcaattgta
    22441 tctcaccagg gaaaatttct gccgctctta cagaagcagc gggggatgaa gtagaacttg
    22501 atatctcatc gaacggtggg gatgtcctag cggcaagcga aatatatacc gctatccgtg
    22561 cctatccggg aaaggtatcc ggaaatgttg tgagtattgc agcaagcgct gcaagtgtaa
    22621 tcgcttgcgc ttgcgaaccg cttagaatct cacctacggc acacatcatg attcataacg
    22681 catgggtgac cactagtggc aacgctgagg aattaaaagc caatgcagaa atgttaagca
    22741 gtgtggatga gtctattgtt aatgcttacg agatcaaaac aggactcgat cggaaaaaac
    22801 ttgctgattt aatggcgaaa gatacttggt taaatgctca aacagcagta gcagaagggt
    22861 ttgcagatga aattatgttt gcagaagcac cagtaacggt actcaatgcc tctcaaccgg
    22921 ttattccaaa aaacgcagta actaagttga aaaatttaat actcaaagcg gaagcaccgc
    22981 aagaagaaac actcttacag aaaaaactaa aagccttaaa tggagggaaa aacgaatgaa
    23041 tttagaacaa ttaaaaaatg cgtgggtcga ggcgggaagt aaagtctctg acttaaatgc
    23101 acaactcaat gcagcattgg ttgacgatga aaaaacagaa gaagatgtag taagtttgca
    23161 agcacaagta aaagcagcac gggctaaacg ggacggattg aaagagcaag tggcaaatat
    23221 ggaagccgaa caagtcttaa acgtcaaaaa agaaccatta gataaaaaag atgaaaactt
    23281 gaaaaacaag tttatcaaag actttaaagc gatggtcaat ggtgatcctg ctattatggc
    23341 tactttgaca tctaatacgg atgaatctgg taatgctatc ggattgacta ttcctgtaga
    23401 tgtgcaaacg actattcata ctttggttcg tcggtttgac tctttgcaag aatacgtaaa
    23461 cgttgaaaaa gtgaccacta ccagcggttc tcgcgtttat gaaaaatggt ctgatattac
    23521 accgttgact gctttggata ctgaagacgg tgaaatccca gcaaatgatg atcctgcact
    23581 ttacttgatc aaatacttga tcaaacgcta tgcaggtatt tccacagtaa ctaacagctt
    23641 gctaaaagat actgccgaaa acattttggc atggttgtct aaatggatcg cgaaaaaagt
    23701 agttgttact cgcaatacaa aaatcttggc agctattgat ggaatcaaag cggcacaaaa
    23761 gaaagatgtt acagatgttg atggaattaa agatatcgta aacgtccaac ttgatccagc
    23821 tatcgaagct acatctatgt ttattacaaa ccaagatggc ttcaatgttt tagataaagt
    23881 gaaacgtgct gatggatctt acttgttaca aaaagacgta gcttctgcaa ctggatatac
    23941 cttcttaggt aaaccaatca agaaaattgc ttctcgtttc ttgccaaaca aaggtacgca
    24001 agctactcct aaatatccac tgtacattgg tgatctgaaa gaagccgtta cattgtatga
    24061 tcgcgaaaac atgagcttgc tgacaacgaa tattggtggt ggagcttttg aaacagacac
    24121 cactaaagta cgcgtcatcg atcgcttcga tgtgcaacta gttgatgatg aagcggttgt
    24181 tttggctact tttacaacta ttgcgaacga gacaccggcg aaagtttaag gagctgattc
    24241 cttatgattc ttgatcctaa aatggattta ggcgaaatca aaaacgcact aaagattgat
    24301 accgatgatg acgatgtgga agtaagccgt gcggcacaag ctgcaattgc atacattaga
    24361 ggggctatcg ggaatgataa gccctctttt tataagcaag aaaatgaaac ggttgatctg
    24421 attaatttag ctatcctgca attagcagat cattattaca acgctcgatc agcaaccgtg
    24481 agtgggaact tgcgagagta cgatttaggt tttacaagcc taatcttgca actcaaagca
    24541 agttatttgc tttttgtgga ggaggagtag cgtatgcccc ttatccaaac aggaaattta
    24601 aatcaacgca tcaagtttgt ccgagatacg acggttaagg atgaggacgg gcaagttgtc
    24661 ccgacttcta caaccattct tacttgctgg gcaagtgtgc agacacaacg cctgaacgat
    24721 attaagacgt cgattggtac ggctttggaa ggaacactga cgttcattat ccgctaccaa
    24781 caaaaatcag agctgaccaa tgatatgaaa gtgcgttgga atggaaaaac gtttgaaatc
    24841 attacgatta cgaaaggcga gtttgcgaag gacttcacga caatcattgc aaaagaggtt
    24901 tcaaaatgag tgtagaagtc gatgcaaccg aagtgtacaa agcgcttagg gaagtaaaag
    24961 caaacgttca acgagtggaa agcccagcac ttagaaaagc tggggagtac gctcaagaag
    25021 agttacgaca aaacacacct tactgggatg gaacgaagtc aaacggtaaa cgtggttcgt
    25081 atatgcaaga acatgctaag aaccatgtgg ttacaagctc gataaaaaac ggattggtag
    25141 aagtcggcta tgacaaagat gtttcttggc ggatgcactt tatcgagttc ggaacaatca
    25201 aacaacgtcc aaaaggtttc gtacaaaaaa cacaaaagca aatcgaaaaa caagtaacac
    25261 aaatcattgc tgacgaagta aaaaggaggc taggactttg aaaacggcag tatcacaagt
    25321 ctattcaatt ctgaatagca atgaaaaaac aaagaacatt gatttttaca ccaatagtgt
    25381 tccggaatca gctcaaacag tacctagcct tccagttggc agaattacag agatatccgg
    25441 caactatgaa gattttgcaa gcaacaatcc tttgaccatt caatttaacg tacaggtaga
    25501 tgtatgggtg tcaaccatga aagaggttga tgccttttat tttgcccttg atgaggttat
    25561 gagggggaat ggttggcaat gcgcatacac ggaacaaaca gatgacgagg acttggaagg
    25621 tgcaaagcgg attatcaaac gatatgtagc aaatatttca ctaaattaaa aggagagaaa
    25681 atagatggca acagtaggat tcgagagcgt catttttggc gtaaaaacag gtgcaggcgg
    25741 cactctaaaa gaattagtag cagataagtc gaaaggcgga gcgatcgaag ctaaaattac
    25801 tggattaggc gcaacttcta acacaacata cgcttcaaac gtaccgttct tcattgcaag
    25861 taaaggggtt tcgtcgccaa aagttacgct tgacgtggca gacttaatgg ataacggcat
    25921 ttacagcgaa atcattggtg ctaaaaccgt ggatggtgca aatgtaattg gttcagaaac
    25981 tgaagcgcct tacgtgtcgg tagtcatggt tacagcgaac aaagaaggaa aacgcttgtt
    26041 catgggattg acaaaaggaa aattcagtca tccagatatc gacatgaaaa cagctgaaga
    26101 caaaggggta gaattgcaaa ccgattccat cgaaggggaa ttcatttctg atgaacgtgg
    26161 ctatgtatac ttaacagccg tagaatcaga aggaatgacc ttacaaaaat tcaaggactt
    26221 ggtaaataac aaagaggggg agtagttaac cctgcatcta caccaacgac agatacaggg
    26281 acaccaaaag aaccagaacc aaaaattgat acacaaggtt agccattttt ggctagcctt
    26341 attttttgta aaaacaagga ggaaaacaaa tgattgaatt gcaattgaaa cttgacggaa
    26401 agaaaaaaac attcaaacaa caagatattt ccgcacgtgc aatgcgtgag tgtatcaaat
    26461 tttacgagaa agcggaaaaa gcagacctaa ctgatttaga agcgattgat tcaatgattg
    26521 caattacagc agatattttc caagatccag cagttacatt tgatgctatt ttagacggtt
    26581 tgactgcgag cgagttagta ccggcattag aaagtgtttt tgaacaaata aatgaactgg
    26641 gaaacaatga aaaaaagcag acggcgagca aaaagaaata agtttttctg aagctaggaa
    26701 agcaatggat caaatctaca aagatttaat cgaagcaggt tggacgatga gagatgtgga
    26761 cgaagccgac tatcattatt tgttacacct ttttggagaa gtggagagtg gcgaagaata
    26821 tgtagatggt gctgatttca tcaaacaatt tttatcggct gaagacttag taaaacttga
    26881 ggaaggaggt aaataatggc aggaaaagga caaccggcag gaaatatcaa gctagggatt
    26941 agtttagata gcactagttt tggtaacacg ctggacgaaa tcaatgcgaa agtcaaacaa
    27001 gctgagtcga atatgcgtgc caatctaaag gcttatgatt cagcaggacg ttcatacgaa
    27061 gcacttagtc aaaagacgaa agacttgtct acggttatgg aagggcaaaa cgccaaagta
    27121 agagaattaa caaagcgccg tgatgaagcg attagcaagt atggcgagga atcgaaacaa
    27181 gttgctaacc ttaacacaca gataaacaat gctaccgcaa aatataatgc ttacagtcgc
    27241 cagttgaacg acacaaaaaa agaattggtg tattccaaaa cagccgtcaa tgatttatct
    27301 aatgaaatca aagaaaatga acgacaaatg aacgccgaag taaaagcgtt gaaagccgct
    27361 ggtgatgaat ctggtgcttt tgaagcaaaa caaaaagggc tagccaaaca aacggaatta
    27421 tccgagaaag ctatcgaaga acagcgcaaa gttgtgaaac tgatggctga tgagtttggc
    27481 gattcagcaa atgaaaccga agatgcaaaa agggcattag aaaagttaga acgacaaagc
    27541 caaatatcta gcaggcaatt agaagcactc aaaagctcca gcgatcaatc aggaaaagaa
    27601 atagaagatt ttggcgacaa gtccacaagg tcagctagga aactggacgg actaaaagac
    27661 aaattaagct cgctaaaaag cgcattttcg tttggtgcag ttgctggatt agcgcataac
    27721 gctattagca gtgtagtaag tggcgtgcaa ggtttggttg gcgaagcagt aaacgcatcg
    27781 gattcattga tgaagttttc caaaaccatg gagtttgcta actttgggaa gtcacagata
    27841 gaaagctcga aaaaagaaat gaaagactac gccgataaga cggtttatgg tttagaagaa
    27901 attctgaaca caaccgcaca attggcatct aatgggattc ctaactatac agaactaacc
    27961 aaggcggcag gtaacttgaa tgccgttgca ggcggttcta gtgatacatt caaatccgtt
    28021 gccatgatgc taacgcagac ggcaggagct gggaaactaa caactgaaaa ctggaatcaa
    28081 ttagcagatg cgataccggg tgcttcagga ttgttacaag acgctatgtt gaaaaacgga
    28141 gcttatacag gaaacttccg tgatgcaatg gcgcaaggtc aaatcacttc cgacgagttc
    28201 aaccaagcaa ttgtacagtt aggtatgaat gacggagcag ttaaggcagc cacttctaca
    28261 gacacattga gcggttcttg ggagcagatg aaatccactg taataaatgg gctacaaagt
    28321 attatagaaa aaataggcgt tgaaaatatc actggtttca tcaacagagt aacaaaaggg
    28381 attgaaaatt ctattcctaa aattactcaa tttataggtt ggttgagaga tattggaacg
    28441 tggatcgttg aaaatagaga gccactaaca tggattgtcg gaatcatagg cggaattaca
    28501 ttagcagtaa aagcattgaa cgtagcaagt atgttgctgg caattactgg cggaacattg
    28561 gcagcccctt ttgtggcgat tggtgtagca ttaggcgcac tagcaggtgc tttggtgata
    28621 gcttatacaa aatctgaaac atttagaaat atagtcaatg cggcttttac agctgtgaaa
    28681 aacgtagtta tgagcgttat caataatttg gtggaatact acaaaatgtt gtggagcgtg
    28741 ttgcagtggc tttgggaaaa aataaaagaa tgggcttcat ggattggcaa taaattcatt
    28801 gaaatgaaga acagcgttgt gaatacggtt cagaacctat ggaatggcgt gaaaaacttc
    28861 ttcagcaatg gcgttggaga tacgtggaat aaggtagtcg gttgggtaaa aaacattttc
    28921 aacaaagcaa ctgaattgaa gaacaacgtt tctgatgtaa tcggtaacct gtggaatggt
    28981 atcaaagaca cattccgtag aggtatcgat acggtattca attggttttc agaactacca
    29041 acgaagatga agaatgccat tattggcggt aaaaacgcca ttgttgatgc gttcaaaagt
    29101 attttcaacg cagcacttaa agcgataggt aaaccagtta acgcaatcat ccatggagct
    29161 tcatgggtac tagaaaaatt gggcgcagaa cctctacaag aatgggatgt accacaatac
    29221 gctacaggta caccagcagg tgggcaccca atcaatggtc caatgatggt taatgatgga
    29281 cgtggagcag aaacagttat cacaccagat ggtagagcct tcattcctaa aggacgcaat
    29341 gttgttctaa acgcaccaaa aggaacgcat gtcttgacag cagaagagac cgcgcaactt
    29401 caaggttcta aagctcctaa gtatcgttac aaaaaaggta ctaacttctt tggtaacatg
    29461 tgggatagtg tgaaaaaggt tgctggtaat gtaggtcaca caattaaaaa cgtagtaggt
    29521 gacgtgtggg actttatttc agacccggga gcgttagcta gaaaagtact tggggggtta
    29581 gatgtattag gtggattgac aaagtatcca ttagaagtag gtaaaggcat cctatctaaa
    29641 gcaacaagtg cactgactga aaaaatcact gggttgttct catctggtaa cttagatacc
    29701 tccgtaggaa caaatggcgt ctataaatat ttggctgatg ttgctaagtc tgtgatgaag
    29761 aaattcccag gatttgtggc aactagtggg tatagaccta tagaccaggt gacccttatt
    29821 cacatggtaa acgtaatgcc attgatattg cattaccagg cgtcacagga ggctcaccta
    29881 gatacacaga agcagcaaac tacgcttttg acaaattcgc ttccaaaatt ggttacgtaa
    29941 tcactaatgg gaaagttcgt gaccgttcag gacaatcagg tcaaccagca actggtgcat
    30001 gggagccatg gcccgatgga gatcactatg atcatgtgca tttaaacggt gtgaaagacc
    30061 cacaaaacac tcaaatttca ggagatagcg tgggaggcag tggggtagaa agatggcgca
    30121 atgtagcaat tagagcgttg aaaatgaccg gtcaatacag tactgcaaac ttaaatgcat
    30181 tactaaatca aatgcgtaca gagtcaaatg gtaatcctaa tgcagttaac aattgggata
    30241 ttaacgccaa aaatggaaca ccatcaaaag ggttgctcca agtgattgac ccaacattca
    30301 gacagtatgc aatgccagga ttcaacagca atatttttga cccactatct aacatcttag
    30361 cttcaatcag atacgcacta tcaagatatg gctcactaac aaatgcctat cgtggagttg
    30421 gttacgcaaa cggtggaatt gtaaaccaac atcaaattgc ggaaatcgca gaaggaaaca
    30481 agccagaaat tattattccg ttagataagg ctaaacgatc aagagcgatg caattattgt
    30541 ctatcgcaat ggacaaacta ggagtttctc ctaaaactta tggaaatgcg acaactgttt
    30601 ccagtgatta cgaaatccta gaagccattg aaaaacaggc ggcaacaacg aatcagctgt
    30661 tatcgttatt gctggcattt ttcaaaggaa atagccgaac tgatagagat ttagctttag
    30721 atattcagaa gatcttggtt aggaggatgt agatgcgaac tgtattactg agaaatagaa
    30781 taaacgaaga aattgatttg tccacagaag actattttgc tactgggttg agtaatatgg
    30841 ggtttgaagt aaaaaaagaa catgtgggac agtgggggaa tttcagagaa agcagtgaga
    30901 gcgttgaaat atctgaattt cagtcatctg taatcatttc tgtgcatggg tttcgagaaa
    30961 aagaactgta caattcactt gtgcagttcc tatcggaagg tccgtttgaa ctggaatttg
    31021 cttttgacgg cgaaacaatg gtaagaagat gcagtctaaa atctttaagt aagactgaaa
    31081 tcgatccgaa aacatcgtta ctaacagaca ctttagaact atatttcaca tcaaactggt
    31141 attccgtaaa acgcgaaaaa ttaattcagc gaccgaatgt agtgaaaaca cgtggtaagg
    31201 tttttccgta taaaagatct tatatctaca ctcaaaactt gtgggagaaa aaaggtgtat
    31261 tcaaattcaa taataattct gtatacctaa cgaatagcaa ggaacggatg tccccgctaa
    31321 aaattcgtgt gattgggaag tgttcaaatc cgtattggga agtaatccaa aactcacaaa
    31381 tcatcgcaac ggatggatac ttcatagata tgactgaaac acagactcta gaagtatcaa
    31441 gcctttttga agatacaacg gcgattttaa aagatattgc aggggtagaa tcttctgtct
    31501 accaacaaca ggattataca aaaactaatt ttgttcaggc cccaactgga gaattcagca
    31561 ttgtgtttca tgttgggggg gcagacgtag agattgagtt atacgaggag cgtgatctgt
    31621 tttgatttta gcagtcacgc tttttcatcg tgatttaatg ctttataacg aacatttatt
    31681 ctctaacagt tttgaatttg gtgtggacga gatcaacgaa gaggctagta gctttacgat
    31741 ggataagtac gttcctgtaa aaacaggcga ttttctacta gcaaaatata ttcctagtgg
    31801 aaaatttgca tattttggtg taattacgtc gcaggaagac gaaaaaatca gttgtaaaag
    31861 cttacttagt ttagctgata gtgagatacc gactgcacgt gtgtcaggag ataactacga
    31921 agagcatatc cggcggttga ttgaatacta tttgctgaat gatccaacga aacaactaaa
    31981 agatatttta gacgtcaaag cagaaagtgc gacctctcat tcgtatcaag ctaccgatac
    32041 gaataaacac aagttaagtg cgtatattct caatggtttc aaaaaataca acgtaaaatg
    32101 gtatttcaag ggaattcaaa acagaaaaat ttatacaggc atacgtgctg taaatgaatc
    32161 aatttacatt aaagacaatt cttctgaatt tagcgattgg gatgtgtttg ttcaggcgcc
    32221 gggcgctgga aacgagaaca agctattaat cgttgataaa gcaatgaaag atatagagaa
    32281 accgataata ctgtcaacat ggtatttgga cgaagaaaac aatttgacac aagatggatc
    32341 gaaagaaaat atcacgaaac caactgtcaa tttggttaat atctacgatc aaaccgcaga
    32401 agataaagca tcctacgaag atgtggcaaa atcagagttg aaaggcaata cgtattcgca
    32461 tgaaatcaaa gtgaacgttg taagaaatgc aaaaaatttg aatgtcgaaa cgattgaaac
    32521 agggatgttt gccacgattt cttataaagg aaagatatac aagtcggttt tgacagcttg
    32581 gcgaatatca agtgataagg agtttgtgga attgactttc ggaaacataa gaagtcgttt
    32641 tatggattat tttgaagata atgggggata aaaaatggtt agcaatgtgg atggatatca
    32701 atttgaaaac gtgaaagtaa gcgcagaaaa tgatgctaga ctttatcacg ttttatataa
    32761 tcggaaaaat caggttattg atggttacga tcagtctatg aatttatctt caagcggatt
    32821 aacagtaaaa gttgctgcag gagcagcgat tattcaaggt cgtatggtcg ttgttcgaca
    32881 agaagaaagt ataacagttc cagcaaactc aagtggttat atagcattaa cagtggattt
    32941 gacacaagaa gttatacctg gatctattct tccagaatcg gaagaatatg aatggactaa
    33001 taatcaagtc aagctagaat ttataacaaa agttataaaa ggtaatttaa acaatggtga
    33061 taaggtctat aatttaccac tatgctcagt taattctact ggatcaactg tttcaatctc
    33121 aaagatatcg gatagttacg agctgactct ctctaaagga gaaattttgt ggagggggac
    33181 tgcgttaatg catgatactc aaactgtcca accttcaaaa aaaatttggc agacagtttc
    33241 aggttttttg ttagtatggc tcccatacga aaacgggcaa gcaattgagg atagatatgt
    33301 aactacgcct ttttataagg agcgtgtaac ttttactaat gttttaggag aaattgtttc
    33361 aggatttgat gactatcaca aaaagtggtt tagtaaacga ataaactata attcgaatac
    33421 taatatattt acaggtgcag caagcaatgc aagtggagat aacgcaaata tggtgcttag
    33481 gtatatagtt tctttttagt taggaggtat ggaagagtgg caaatttaga aattaaatta
    33541 tctgcaaata agagacagcc ttatctacgt caccgtgttg ttggtagagt tggtgatggg
    33601 gggctcacaa caatcaatgt acaacttctt gaggaagatg aaattacacc ttttgtaatt
    33661 aatctaaacg gtactttgaa atttgtgggc gaagtttcaa acggtaacta taccgaggga
    33721 gaaccagaaa taatcgattc gactaatggg ttaattagtt acacgttcac taagtcaaat
    33781 ttcagcacga gtcatcaatt caaacaagca tattttgaat atgtagatcc taacggcaaa
    33841 aaagtaactt ttcagaactt catcatagac gtgttagaac gagtggatat taattcggag
    33901 caagcgaaat actatatttc ttcattggaa aaattacaga gtgaaatgca aaccactttc
    33961 aatcagttca ttagtgataa acaggtccaa tacgatcaaa tctactcgaa atataacgaa
    34021 ttagtaagat tgataaatga atcagataag caagtaaatg atcgtattga tcaaaccaat
    34081 cagcaaatcg gcgatctcgg caagctgaaa aagatgtaca gtaacagcat cgactttggg
    34141 ggctatgatt attcggggaa tccgaatttg ttaagtaagc tatcatacga cttaattgaa
    34201 aatcaaaata cttcagctgg aacactttct aagggtgaaa actcgtttaa atataataag
    34261 atatcagctg aagtggaagg tggagtagag ttatattata aacgaagagg tatagctaac
    34321 tggttaccct ctaataaaac gcttgtaatg actgttaagc ttagagctgg agcggactat
    34381 agtccagttg acggaaaact tatactgatt agatataggt atgttgacag tggaactggc
    34441 aagattgttt tagacttacc tattaacagt aattcgataa ctcaggaatg gaaagagttt
    34501 agtattactg gaactactcc aacatttagc ccacaagcat accatccttg gatacaattt
    34561 agggctcaag atggggtact tggggaaata gaaatgagct atgacatcaa aatcgaagaa
    34621 ggctctacag ctacaccata tcagcctaac ttacttgatg actcttactg gctaggtaaa
    34681 acaccgttgg gtgagaattt gataacgaat gccaagttcc caattataac acgaagcaat
    34741 cctatttctg gttttgatat ttcagaagaa ttaataatag gtgaaactta tacggtatct
    34801 ttaaaaggga ctaagccagc tactaaagaa ttccacttat actacggaga agcaaattac
    34861 cagcagttgc agtatcaggc aactttactt cctgtggaag gattaacaga tgtgtggagt
    34921 gccactttta cagccagaaa tactagtgaa acgacaaatt tacttagagt agcgttatgg
    34981 caaaaaccta attctgcaac atatgatact gttcaaattg actggctcaa gattgaaaaa
    35041 ggcgacacac gcacaccaaa tatcagtcag tttaaatact ttggtgaagg cttgaaagac
    35101 agtaacaatc cgaacgacta cagctgggat gtcacaccag agtatactga aaaaagcttg
    35161 aataatacgg ttagtctgac tgaaccgcaa tccatcgaag gattgaaaaa tttcgaagat
    35221 ggtattcaat caaaaggaaa atctgtattg acatcagacg acaacaaata tgaagtcgta
    35281 accttaacag ttacaaacgg gaataccgga tcagcaaagc tttatcgtga aggaaaaacc
    35341 gtcactattt atttttttgc gttgaatgg
//
LOCUS       DACDUU010000024        34722 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.024, whole genome shotgun sequence.
ACCESSION   DACDUU010000024 DACDUU010000000
VERSION     DACDUU010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34722
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.024"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            160..840
                     /locus_tag="IWL30_001201"
     CDS             160..840
                     /locus_tag="IWL30_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP9705979.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            930..1964
                     /locus_tag="IWL30_001202"
     CDS             930..1964
                     /locus_tag="IWL30_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP9705980.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            1978..2313
                     /locus_tag="IWL30_001203"
     CDS             1978..2313
                     /locus_tag="IWL30_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705981.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            2297..4777
                     /locus_tag="IWL30_001204"
     CDS             2297..4777
                     /locus_tag="IWL30_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705982.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            4833..6392
                     /locus_tag="IWL30_001205"
     CDS             4833..6392
                     /locus_tag="IWL30_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9705983.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            6389..7753
                     /locus_tag="IWL30_001206"
     CDS             6389..7753
                     /locus_tag="IWL30_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9705984.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            7924..8994
                     /locus_tag="IWL30_001207"
     CDS             7924..8994
                     /locus_tag="IWL30_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9705985.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            9009..9677
                     /locus_tag="IWL30_001208"
     CDS             9009..9677
                     /locus_tag="IWL30_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9705986.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            9697..10275
                     /locus_tag="IWL30_001209"
     CDS             9697..10275
                     /locus_tag="IWL30_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9705987.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            10676..11587
                     /locus_tag="IWL30_001210"
     CDS             10676..11587
                     /locus_tag="IWL30_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9705988.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(11965..12882)
                     /locus_tag="IWL30_001211"
     CDS             complement(11965..12882)
                     /locus_tag="IWL30_001211"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAP9705989.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            13084..13521
                     /gene="zapA"
                     /locus_tag="IWL30_001212"
     CDS             13084..13521
                     /gene="zapA"
                     /locus_tag="IWL30_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAP9705990.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            13579..14127
                     /locus_tag="IWL30_001213"
     CDS             13579..14127
                     /locus_tag="IWL30_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAP9705991.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            14242..16602
                     /locus_tag="IWL30_001214"
     CDS             14242..16602
                     /locus_tag="IWL30_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP9705992.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            16689..17003
                     /gene="trxA"
                     /locus_tag="IWL30_001215"
     CDS             16689..17003
                     /gene="trxA"
                     /locus_tag="IWL30_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAP9705993.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            17185..18963
                     /gene="uvrC"
                     /locus_tag="IWL30_001216"
     CDS             17185..18963
                     /gene="uvrC"
                     /locus_tag="IWL30_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAP9705994.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            19108..20007
                     /locus_tag="IWL30_001217"
     CDS             19108..20007
                     /locus_tag="IWL30_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9705995.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            20000..21262
                     /locus_tag="IWL30_001218"
     CDS             20000..21262
                     /locus_tag="IWL30_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9705996.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            21423..23321
                     /locus_tag="IWL30_001219"
     CDS             21423..23321
                     /locus_tag="IWL30_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAP9705997.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(23651..24370)
                     /locus_tag="IWL30_001220"
     CDS             complement(23651..24370)
                     /locus_tag="IWL30_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAP9705998.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            24707..25039
                     /locus_tag="IWL30_001221"
     CDS             24707..25039
                     /locus_tag="IWL30_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9705999.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            25026..27029
                     /locus_tag="IWL30_001222"
     CDS             25026..27029
                     /locus_tag="IWL30_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAP9706000.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            27048..27518
                     /locus_tag="IWL30_001223"
     CDS             27048..27518
                     /locus_tag="IWL30_001223"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAP9706001.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            27539..28123
                     /locus_tag="IWL30_001224"
     CDS             27539..28123
                     /locus_tag="IWL30_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAP9706002.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            28138..29124
                     /locus_tag="IWL30_001225"
     CDS             28138..29124
                     /locus_tag="IWL30_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAP9706003.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            29114..29425
                     /locus_tag="IWL30_001226"
     CDS             29114..29425
                     /locus_tag="IWL30_001226"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAP9706004.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            29447..31228
                     /locus_tag="IWL30_001227"
     CDS             29447..31228
                     /locus_tag="IWL30_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAP9706005.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            31221..32597
                     /locus_tag="IWL30_001228"
     CDS             31221..32597
                     /locus_tag="IWL30_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAP9706006.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            32604..33239
                     /locus_tag="IWL30_001229"
     CDS             32604..33239
                     /locus_tag="IWL30_001229"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAP9706007.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            33269..34624
                     /locus_tag="IWL30_001230"
     CDS             33269..34624
                     /locus_tag="IWL30_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAP9706008.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
BASE COUNT        11484 a         5976 c         7444 g         9818 t
ORIGIN      
        1 ttaataaaaa aggaagaata ttcatgtaaa cgattttatt gtttttttct cttttataca
       61 aaaagggtca taatttcaag taagcggatg aattcatgag gattttttgc atactgtctc
      121 tactatactt aggtcactaa gaaaaaggag tgatttatta tgaaaaagaa catgattggg
      181 acattattac tttcaacact tcttctaggc ggcttggcaa caccagcttt tgctgagggt
      241 caagcgactt caaaaggaga tattaccttt actgaaccaa caaataccgt ggagcctctt
      301 aaccctactg atccttcaaa accagtagag ccagcagatc cggaaaatcc agcaactggc
      361 cagacaggtc ctttaacact ggatgtagtt cccgaattgc catttggtac acacgagatt
      421 gaaagcggaa caaaaacgta tcaagtagac gcttcaaaaa acgatacacc ttatctccaa
      481 gtatcagatc gtcgaggtgt aggtgcagat ggccaagcac aagggtggaa cgtgacagtt
      541 tcggtttcag attttgtaaa cggttcacaa gttttgcaag gcgctgaatt agattttgga
      601 acatccactg ttaaaagtac gtcagataac gaatccactg gaccaactag tcaaactgta
      661 actgggttga gtaaagcaag tgcagcgaca ccaatattta cagcagcaaa agatcaaggt
      721 ctgggaacat ggctttctgt ttatgatcca gcaaatatca cactgaaagt gccgaaagca
      781 gcagctggaa cattcacggc tgatttgact tggaacctcg tggctggccc agtagcatag
      841 agtatgaaat aaattcaaca aactgcggtg aaaatactat aggtgaagga ttttttaccg
      901 cagttgttgt tgaaaggatg tggtggacaa tgaatcaaag taaacgactg tttgtttcgt
      961 ttttttctat tttaagtcta tttttcattt ttccgtcaat cagccaagca aaggattcag
     1021 caggagactt tggaatcaaa ccggtatttc cagagaacca aatagataaa gcaatcggtt
     1081 attttgattt attagtggca ccggaacaaa atcagacact agaagtaatt atcagcaatt
     1141 cttcagatga agaacgaaca tttgaggtct ctgtcaatcc ggcggtaacg agtgatggtg
     1201 gaacgatcga ttatagtcaa aaaaacccaa cgttagatga gacattgccg tttgatgtcc
     1261 gtgacgtttt gctcattgcg aaaaaggaaa tcaatgtatc agcacatgca gaaacaactg
     1321 tacctataga agtgaaaatc ccagcaaaaa gctttaaggg acgtgtatta gcgggtattc
     1381 atgtttcacc taaagaagaa gctgaaacag aaaatgctac agaaggagca caaatcaaaa
     1441 atcgtattgc ttataatctc gcagtcgttt tacaagaaag tcaagagacg atagaaccgg
     1501 atctgaaact tttatcagga gatttggacg aggtcaacgc aaagccaact gttcagcttc
     1561 gtttccaaaa tcctcagcct aggattatca gcaatttgat atttacgagc aagatctttt
     1621 atgaaaatca gctttatatt gaaaatacgt ctaatgcatt tttagttgca ccaaattcga
     1681 attttcactt gaatcttgat ttagctggtg ataaagcaaa agccggcgat taccgcgcag
     1741 aaatcatagc aaaatcaggc gacagtaatg aatggcgatt tacacaaaac tttacgatca
     1801 aaaaagaaaa agcacaaaaa gtaaatgaaa attccgtttt tgcagtacaa gaacaatctt
     1861 tcccatggct ttatgcagga ttggttgctg cagttctagt gttatggttg attttgctgt
     1921 ttttgtgggt ccacaagaaa aagaaaaaag gagaaacaac atgatgaggt gatttcgttg
     1981 aaaacatcga tcttattggg agggattatt gcaagtttgc tgttcttcag ctggcaagag
     2041 ccagtccaag cagataccaa aagttcaggt acgattacat tttatgatct aagtccaatt
     2101 ccgcatccaa gtggttcagt caatggcgca tcaaaacagc agacacagct accgatttat
     2161 aaagaatcta atcaacatgg cgtattcccg gagacgaatg aacaaaagat ccgttttctt
     2221 tatttgatca ttggtataga attatggctt cttgcgtggt tgttgttatg gagaagaaag
     2281 gaaaaggagg atgaacatgt tgctacaaaa taaattttca ttttttcgct ccttcttttt
     2341 tacttcgctt tttgttttaa gtatatttat cttttcacct tcggcagaag ctgttggagc
     2401 aacgcctcct gccatttctt atgatccata cacgtatgaa aatatcgtac ctccaaatgg
     2461 tggacgtagt gcagtcaatg gcgggaagct tgtccaacta actggggcta accaattgcc
     2521 taaattgact gctacttatt tgaacgatgg ttcaaatgat ccttggaaag gtggcacggt
     2581 ccgttttcat tgggaaagcc atgtgaaact aacaaatgta cttgatttgg tgaatatcac
     2641 tgcacaagga tttgcatttg tggcgatcat caatttgccg gaacatgtat caagcagtga
     2701 tgtattaaaa gcaattgact ggaacaacgc ctacttgtat attgccggtg cccccatcaa
     2761 ggttactagc ggaagtttat tatcagcagg aagccatacc ttgcgttttg ggatggggtc
     2821 gtgggataca aatactctat tgacagatat tattgctatt attacgcgag gcgaattcaa
     2881 cgtggacgat atcccgcttc aatttgatgt tacagctaat gtcgcgaaga tgacagaaaa
     2941 cggagataaa tatgatacga gtccaaacaa aattttgacc aagggtaaat tacaaccttc
     3001 tatgtctaaa aaggccgttt tttctgttga tttttatgat tcgaataata ttatccaaga
     3061 tcgtatgttc aattcgattt tagctccagc tgcaggtaga ttttatccta aattaactgg
     3121 cggaaacttg aatccaggag catatgcatt ttctgcaagt gcagagataa atacgtggaa
     3181 tagttacatc tcaccaagtg attcaacagg aaaatataat acatttgctg ataccacaga
     3241 atacgtggat ggaaataacc agaaccttcc aggctctacc aatagtcgga cattgacgat
     3301 ggggctgact gaaggacgtt ttgatcagtt gccatttagt cgctttgatc gcgtagtcaa
     3361 ctattttaca ggaaaaaacg ttacacaagg agcaatactg actcatactc cagcaagcac
     3421 aagtggattg aatcagacag ccaatatcgt gtacagtggg aaagatgcgc aaggcggatt
     3481 gctttctcct gttgctttaa aggtattgga gcagtacaag ctagacggaa cggttttacc
     3541 aacaaactta acagccgata gtcagtgggg aaagctcctt tcttctgcat cgtaccaagt
     3601 cacggccaat tggtcagcaa ctgaactaaa aaagggttcg atccattaca ggctttattc
     3661 gaaaggaacc caaaaattag cgacaggatg gacagatcaa cttttccaaa cgatccaatc
     3721 aaataacggg ggtgtaaata cagctacttc tacactacct gcattggctt cagggcaata
     3781 ttattttgat tatcgcttgg tggacgatgt acttactaac atgtatccgt cttttgcgta
     3841 caaatggcag tctgaacaag ctggaatcac cactcttcct caaataacag tggctaattt
     3901 tccatctgtg agtgcagata gtcagctaaa aaaccttagc agacaacctg agacaggaga
     3961 tcccctgtct gctctatcag gagatacgat tcaagaaaat gtgacattca cgttaacaaa
     4021 atctggggat gcgataaccg acaaaaaaat cacgatctca cttcctgaaa atactactta
     4081 tatcaatggt tccttaaaat taaatggtac aactatcgca gataccggca ttcaacaagg
     4141 agtttctgtc ccagcagatc tattgagcaa aataggagat accattcatc ttacttacaa
     4201 ctatcaatta aatactgtag atacttcttt acaatccgtc agcatactga caaaggcagc
     4261 tgtactttcc agcaatatta cattagcaga tggtgctaag cttcctaacc cagttgtcca
     4321 aacatctgct aaaacaattc ttgtccccaa acaagaatta acattagtta atgtaccaga
     4381 tgattttaca tttggtaacg atctccccaa accgttgaag acaagttatt atgaatcaaa
     4441 aggcgatttt tcttttgatg tgcgggatac tcggcttcca agcacgtctc cttggcaact
     4501 gacaggtaca ttgacttctt tatttaagaa tgatcaaggt caagaattaa gcggaacaaa
     4561 attatacttc aatcattctg gatcaaagca attgattcag caaggtcaaa acacgttgat
     4621 ttatgagagt gatggaacag ctaaaggaga ggttctcgtt gactttcccg ataccgatgg
     4681 tttactctta gaggtaaata gcagtacgaa tgctcaacct ggcgcaacgt atcaaggcat
     4741 ggtcacatgg gaactgacag ctgggccaac cagctaagtg attagaattt gtatggatct
     4801 gtgataactg tcacagatcc tctttttcaa ggatgagaag gaaacttctg ctgttaaaat
     4861 cagaaatgct tgaaggagtg gacttggaac ttttcagttt gcttgaaaaa aatgaacaac
     4921 ggcaaatcaa acttttccaa cttttattag aggaaaaagg agaggtttcc gttaataacg
     4981 tacttacccg cttatcagtt gatcgggcta ccttgaaaga agatctgttt tatttacaag
     5041 ctaacttgga aaatatcaaa caattggaaa ttgttatcca agaagggaat attttattaa
     5101 tcagacatgg aaatatagca gcagtcgatg tttattatca ttgttacttg cgctattcta
     5161 caaaatatca gatacttctt tatctgttgg aaaatggctc atttgatcgg cagaaacttg
     5221 tcatggaact taatatcagt tcggctactt tgtctcgcag agtgaaggaa ttaaatgatt
     5281 ctttaaatga gtttcatctg aaaataaaaa gcgggcggct gaccggtcca gaaagtcaaa
     5341 ttcgttattt ttatttccaa ttattttggt ttggacgtcc ctatcttgaa aacaaaaaag
     5401 aatacagtga tttatcagga aacacttttt tagatctgct gcaaaaagag tttagttttg
     5461 ctattacaga agaaggacga ttgaaatttg aaatatggct ttccattatg aagaagcgaa
     5521 tgcgctgtac gaaaaaatcc aaaccagatt atagcaatct tcctgttgga ttgtttcaaa
     5581 cgacacaatt tttattggac ctccatacat ttttgacccg ttacttcttt caatatgcct
     5641 ttccttggaa tcaaaatgaa acgttggttt tttatctttt catgatcagt aactttacat
     5701 tagatacaca taactcgcat gtccaggagt ttttgataag cgaaaaatac aaagacgacg
     5761 tagtagctca attgaatcga acgtttaaac aatcgataga agaatatttc tctttttatg
     5821 tttttccaga agagatttcg caaaagattg cgttgacagt gatgcaaaat cacgctcatt
     5881 ttgcctattt tagaggatgg atgaatattt ttggccaaga aggattaaaa cagcggttaa
     5941 ctgaagtaga agataaacaa ttcctggttt tatgtgaaag attggtcaga caaacatttg
     6001 ctattgttga ttttactgaa gtagacgagg aaaatgcccg ctttgaatta tttgctcgct
     6061 atgcatctat tttacggacg ctgatcaagc agttagaatt acgtctgcaa attgcttatg
     6121 atcttcctta tgaaaggcta atggcggata tcgtgaaaga aatcatccag gaacaattgc
     6181 tgaatacgat caaatatgat ttagtcgaat acgagaaaga aaagcagtat gatatcattt
     6241 tgactagcca attgaaagag tacccgtcac agaagtccgc taaagttttt gtattcacta
     6301 gcaacgaata taattatgat ttcccctatt tgaaccaatt tttaaaagaa tgttatttag
     6361 aaaaattgaa tttaaggatg aataaaacat gaaaatcgtg atcattggtg cttcccatga
     6421 aggcatcaca gctgccctaa atctaagaaa acttcaaccg aaagcagaag ttttactgat
     6481 cgatgaagat cataaagatg gactaggcta tgtttcaaat ggaatcaacc tttatttaaa
     6541 aggaaagata cgtagtttag ctgaagttgc ccataacatg cggactcttc aagaaagtgg
     6601 ggcaaaactg atcaccgaat ggagagtaac tgaacttgat ccagacaaac atcagctcat
     6661 cctcgcatcc aaagaaggaa aaaacgagac cataacctat gataaattaa tcgtagcgac
     6721 aggttcctct cctgtcacct tgtataaaca aattgaagct gaaaatgtct acacctataa
     6781 aaatctagtg caatctaagc aagtacttgc cgctttaaaa gaagcaaaag aagtagtgat
     6841 cttcggtgct ggttacattg gattggaatt agcagatgct ttgagaaata aaggatacat
     6901 tattcatcta gttgattata tgccgaatgt tttatcaaga tatttcgata aggatatgat
     6961 caacagtttt caaaatcagc tgcaaactaa gcaaataaat ttttatccaa atgaattctt
     7021 gatcgattgg aaaaaatcag aagagaaagt agtatccgta caacttctgt ctcaaacaat
     7081 aaaggcagat atggtcattt tttctgccca aactcgtcct aatacgacat tgttaaaaga
     7141 aaaagtggct ttgtatgagg atgagactgt gatagtcaat gaatatctgc agacaagtga
     7201 tccggacatc tatgcgattg gagatatcgt cccagtcagt tttgacaaaa ataaacggca
     7261 tctcttttta cctttagtta ctcgagctgt acatatggca agagcagtag cgctaacttt
     7321 gagtggtcag ccaaccgctt acgacctcag acaaaagata acagcagcag tcattataga
     7381 ctacttttta ggaactgtag ggctgacaga agatgaagcg ccattcttgg aacaaaatac
     7441 cggttcgtgt tcaggagaat ttgatctatt tccacaatac gacgaagaaa acaaaacagt
     7501 aaatgcaaag ctgatctacc atccggatac attagaaata attggcggac agctgataag
     7561 ccaagaattc ttattatcag atttgaacct gcttgcggat atcgtgaaac acaaaactac
     7621 gattccacag ctagcagttg aggattttgg atttctgtct gaatacacct ctcgttttca
     7681 ctacctcaat gagttagctt ttaaagtact agctaagaga gcaaatagca atagagtaga
     7741 taaaagactg tgagaaatat cacggtcttt tattgtgtgg ggatataata aatattaatc
     7801 aatcgagaat aaaaaattca tataatgaaa tagcggaaga attttttgag cataatattc
     7861 gccaaaaatt tatcaagtga taaaattgtt gtataaaatg attatttcag gaggaggagt
     7921 taaatgtttt tagcattaaa tgaaattatg cattcaaagt tacgttatgc gttagttgca
     7981 ggggtgatgt ttttgatcgc ctacctggta ttttttttga ctgggttagc ttatggctta
     8041 gctcaagata atcgaacagc agtcgacaag tgggaggcag attcgattgt gctttcgaaa
     8101 gatgcaaatt caaatctagg gatgtccatg attactaaga agatagcaga agaagtcgaa
     8161 ggtgggaaag tggcgtatct tgcccaaaca cctggagtcg tgactagtaa agacagtaca
     8221 gaagaaggaa aaatcaatgt tagcttcttc ggtatcgata aaaatcaatt catcatgccc
     8281 aatcttgttg aaggaaaagc ttttgataac gatgatgagg cagtaggcga tattagtttg
     8341 aaagaagaat atggtctagc cataggagat acagttaagt tatctggtag tgacaagacg
     8401 tttaaactaa ctggctttac ggatcatgcc aaattcaatg tctcaccagt tctctataca
     8461 accatcaatg cttaccaaga aatccgcttc gaaaaagaag atacaagtga aaatgcacgg
     8521 attaacgcaa tagttgtcag aggtaaaatc agtaatttac ctgaagattt agaacagatc
     8581 aaaatttcta aattcatcaa tgaattacct ggatataatg cgcaagtatt gacttttgga
     8641 tttatgatcg gatttttgat cgttattgcg gctattgtga tcgggatttt catttatgta
     8701 ctgacgatgc aaaagattaa tatctttggt gtaatgaaag cacaagggat cacaggagga
     8761 tttattgcgc gttctgtagt ggcacaaaca ttcattttat catttgttgg tattcttctt
     8821 ggtctattag gaacagtggg aacatccctt gttctacctg atgcggttcc attccaaagc
     8881 aattggctgt tttttggcgt cattagcttg ttgatgttag tagtagctgt gttaggcgca
     8941 ctattttctg ttcggacaat cgtaaaaatc gatccgttga aggcaattgg ataagaagga
     9001 ggaagaacat gaaagcaata gaatttaaag aagtagaaaa aagtttccaa gatggcgacc
     9061 aagtagttaa agcattgaaa gaaacaacca tgagtatcga taaaggggaa tttgttgcaa
     9121 tcatcggtcc ttctggttca ggtaaaagta cttttttgac tttggcaggc ggattgcaga
     9181 cgcctagcag tggctctgta tttatcaatg atcaagaatt cagtaatcaa aaagaaagcg
     9241 aacgcgttaa actacgtttt aaagaaattg gctttatttt gcaagcttcc aatctggttc
     9301 cttttctgac agtaaaaaag cagttattat tagtagataa gattaaacat gaacatcgtc
     9361 aaaaagaagc aaacgaatta tttgagcagc ttggcgtgga taagttgatc aacaagtatc
     9421 cagaagaact atctggtgga gaacggcaaa gggtcgcaat cgctcgtgca ttgtacaatg
     9481 atccgtccat tattctagca gatgaaccaa ctgctagttt ggattcagaa aaggcacgag
     9541 aagtagtcga tatcttggca aaagaatcaa aagacaaaaa taaagcaacg atcatggtta
     9601 ctcacgatac acggctgatt gacaaatgcg atagaatatt tatgatggaa gatggtgtat
     9661 tgaaagaaaa agaataacca aaaagcaggt ggaaaaatgg tccatcgata caacaaagat
     9721 acacagcaaa aaattttgaa agaagcgact gacttgttta tgtccaaggg atatctcggc
     9781 acttcaacaa gagaaatcgc acaaaaagtt gggatcaccc agcccaatct ctatcattat
     9841 ttcggtgata aagaaaaact atatacggca gttttagaaa accacttaaa agatgttgga
     9901 aaagctttga gagaaatcgt tcaaacaagc gaaacgggat ttcaagtaac cttgacaaaa
     9961 atggcgcagt ttttgattga gacacatctg gtcgatttgt ttctgatgct tcacgatctt
    10021 gaaagcaatc tttcaaagga aacaagagat catcttttct ttctatggaa gaaaaattat
    10081 cgtgaacctt ttgaagaaat cttttcagca aaccaatctg tgctaagaaa tgggatttca
    10141 aaagaaatcg cagcaaggca ttttttctta gtgttagcac cttacatcac tcggtcgaaa
    10201 agacctgtgg aagaaccgct aaatgtggcg caattgatcg atttatattt gaatggtgta
    10261 ttaggagaag cgtaaagctg ttatataggg agaaaatagt taggtgaaga agcaagatat
    10321 gacgaagatc gttatatctt gcttctttat ataggtagtt tttcataaat acaaatttgt
    10381 tattctctca tgattatcag ggtatatttt tatttattat gtcggtgttc caggaagtgt
    10441 tttaatttaa taacgaagaa attaattgtt tgtagaatga aggaaactat ttattgggta
    10501 gatgaatcac cacgaatagt caaaaggaga tttcaggata tgccaaaaat gaaaaaaatc
    10561 attttagcag taatgttatt ttctacggtt atatctttta cagccccttt tttgcttact
    10621 taagctgatt ttaagttttg gcccttagtc tggatctacg aggtgagagt caagaatgaa
    10681 aaaaataatt ggagcttttg tatttgcaat cattatcatt attatctgtt ttcaaataga
    10741 tgtttttggt caaaaaaaga tagttggaca aacggtcagc agtacgaaca gtacggttac
    10801 aaagacaaag gaatcatttg atgatacaga acaagagaca ttaatcaaaa tcggaaaaaa
    10861 tgaccgtaca ctatatacag atgactcgct aactacccca cttgcaacta taaacggcgg
    10921 agagctggcg gagtttcttt ctgaaacaaa aaatgcttat cagatcaaga caaatgatgg
    10981 ctatactgga tatttagccc tcgaagatgg aaccaaagtc acaaagaata tacaaacaaa
    11041 accggaagag ctatctgatg cagtgattgt attagatcct gggcatggcg gaaatgatac
    11101 tggggcatta agcaatgatg aacaaacaga agaaaaagat ataacattaa gtacagccat
    11161 aaaagtaaaa aaggcgctag aggatgctgg agcaacggtt tatttgaccc atactacaga
    11221 tgaattagta cagcttggag atatatgcga ctacagtgaa gaaaaaaagg cagatgtctt
    11281 tatcagttta cacgcagact ctacggaata tgcgaatgaa gcaactggaa tcacgacata
    11341 ttattactat ggacaagagg aaacattagc acagacaatt gctgactcct tcactgatct
    11401 gcctctggat tcaagaggaa tctccactgg gaactatcaa gttcttcgag aaaatcttca
    11461 accttctatt ttaatcgaga tgggttatat gaataatgat agtgatttaa aggaattagt
    11521 aacagacagt tatcagcagc agattgctgc aagtttgaca caagcattga ccacttattt
    11581 tcagtaataa ataagcccag ccaaacaaaa atagctcctc atatttttcg tttggccggg
    11641 cttattatcg cagatgcaag cttttaaaca ccgtttaagg aagaggtcgt gagcctgccg
    11701 ttcaactctg agtcacaagg agcaatttca aaaaacagct tctcatgttt gatgactatt
    11761 gtgacttgtg accgaggagt tggcatgaga acaccgttta agaaaaaggt tgtgaaatca
    11821 acgttttgtt ctgagtatta gagaaacaaa cggaaaaata gttcctcata ttttgccgtt
    11881 tgttgagcta ataccgagga tcagatgatt gaacaccgtt tattcggaac aagggactgt
    11941 gacatgactt ttgtcacagt tccattattt ttttagtagc ttcaaagctt tttctgtatt
    12001 ggcaaaatga agtttggcat atttttcaag cataggcatg ccgcctttct ccaatagttt
    12061 tgccgctact tggtcagatt tcgttccagc tcctgacgga agggtgattc cagcttcagc
    12121 tgatgctttg tctaactctt ccaaaaagct ggcacgactg atgatagaag cagcagcaac
    12181 agctaaatga tattgttctc ctttagtaac gaagtacagt ttttcttgta attgattttt
    12241 ttctaaacgc gcatattttt taaaatttgc ttccggcgta aactgatcga tcaaaatcgc
    12301 ttctggtttt tctggctcga ttttttttag cagcaaatgg atcgcttgat tgtgcaaagc
    12361 gactttcatc cgaacggcat tgtattttgg ttggatctca ttgtattttt ctggagataa
    12421 gacaagcagt ttatagggga tggttttttt taagatgtca gcaagctgga tgatttggct
    12481 atcttttaac tcttttgaat ctctaacacc aagtttacgt aattctgcaa gctgatcacg
    12541 tttcacataa gcagcacata ctgttagcgg accgaaatag ctgccatttc cgacctcatc
    12601 actacctaaa acggataatt gactgatatt ttttggcaaa gaagaggctg tttttgctgg
    12661 cttggcttgt ttatcctgtg cctcccccca tttatcagct tctattgctg cattcgtacc
    12721 ttgaaacatc acttttccgg atgtataagc ggtaatggtc gtcccgtctt tttttgcagc
    12781 aaagctagta taaggaatct gtttatttaa caaataagag cgatagtatg ctttcatttg
    12841 actaagttgg tcgtttgttg ctttgataat gacgttgctc ataaaattct cctcgttttt
    12901 atacataaaa ctataccgca actcacgact tttcgttttc taagagattg cgagacatag
    12961 cattttttta aagtttatca tataatagtt aagtatgtga aaaatatttg tggagaacgg
    13021 tgaaagaacc aacttcatgg tacactaaat aaaactttca tcaaatagga ggaaatgatt
    13081 cgtatggcgc acgaaaaaac acgctacaaa gcggtgattg ccaaccaaac atatactatt
    13141 atcggacggg aaaccaagca tcatatggat atcgtaacga agctgatcaa tgaacagtta
    13201 gcagaattga aacaattatc accacaaatg gataatgaac aagcagcaat cttgatggct
    13261 gtcaatgcgc tttcggatca attgaagaaa caagaaagaa tattggagct ggaagaagaa
    13321 acagcagaat taaagaaaaa aatgatcaaa tttacagagc ttgaaaatcg tgtgaaacgc
    13381 attgaagcaa tcgagaacga agctcgggaa gtattgaaag aaaatggtca agcagatcat
    13441 accattaaaa atcatgtgga agcacaacag attttaaacg agaaacgaaa aggacaaatt
    13501 aaacaaaaat cttctaatta agatcctttt atcaaaataa gaaaaattta aaaaagttag
    13561 aagagaaagg aacgtagcat gctgagtttg ctgattttat ttttattgct aattgcgttt
    13621 ttttctggag cacgtcgcgg gtttgctttg caaatcatat tcgcgattgg atatgtattg
    13681 tcatttattg ctgcccagca tttttacaaa ccattagcaa gtcatttgga attatacatt
    13741 ccatacccag ctgtcacccc aacatcaaag ttagcatttt ttgatcaggt atttgccttt
    13801 cacctagatg aagcttttta tgcaggaact gcttttctga ttattttgct aattggctgg
    13861 ctgcttaccc gatttgtggg ggtttttgtc catggattga cgtatgtgcc gatcttgaga
    13921 caggtagact ggattgcagg aggcatcctg tcactaatca tggcttacgt tactatattt
    13981 ttgattttgc agttacttgc ctttgttcca ttagatgtgg tccaaaatca attcggcgag
    14041 catagtttcg ctcgttttat cgttgagaga acgccatttc ttaccaacaa gatgtatgag
    14101 ctttgggtaa cgaatatttt gaattaagga aaatagtgct ggaaaatatt tcgttttttg
    14161 tacagagtag tttatttaag aaggctgcgt agcgtatgtc cggcacagct cttttttcat
    14221 agtgaaagat aaggtgataa aatgaataac cgaatccttg aaacattgga atttgaaaaa
    14281 gtcaaacaga tggtcagaca atttgtagtg actgcacaag gaaaagaaga actggcagaa
    14341 ttggtaccag tgagtgagaa acaaacgatc acaaactggc ttcaagaaac tgaggatgga
    14401 ttgaaagtgc aacgtctgcg aggcgggatc cctataccta aaattgaaaa tatccgtccc
    14461 catatgaaac ggattgagat cggagcagac ttgaatggag tggagcttgc acaagtttcc
    14521 agagtattgt ctacgacaag cgaattgaat cgtttcattg atgatctttc tgacagtgaa
    14581 atcgaatttg cacgtttgta tatgtgggca gatcagttag tcacgatccc tgttttgagc
    14641 cgccgtttaa aggaagcaat cgatgaagac ggacgcgtga ctgatgacgc ttctcctgag
    14701 ttaaagagca taagacagaa cattcggcga agtgaacaag ctgttcgtga acagttagat
    14761 gggatcgtcc gtgggaaaaa tgcaaagtat ttaagtgatg cgatcatcac catgcgaaat
    14821 gaccgctacg ttatcccagt caaacaagaa taccgcggtg tatttggcgg tgtagtccac
    14881 gatcaaagtg cttcaggtca gactttattt attgaaccaa aacaagtggt ggatttgaat
    14941 aatcgtttgc gtcaatacca aatcgctgaa cgaaatgaga ttcaacgtat tttaagtgaa
    15001 ctttctgctg aacttgttcc ccatcgtcaa gaaatcatcc acaatactta tgtgattggt
    15061 aaaatggatc tgatgaatgc aaaagcacga tttggtaaag aagtaaaagc catcgtgccg
    15121 ggaatcagtg aagacaatca tgtcgtcttg aaacaagcaa gacatccact gatcgatcag
    15181 gaaaaagtag tttctaatga catcacaatc ggaaaagact atcaagccat cgtcattaca
    15241 ggacctaaca caggtgggaa aacgatcacg ttgaagactt taggtctgtt acaactgatg
    15301 gggcaagcag gattaccgat tcctgcaggt gaagaaagtc aaatcggtat ttttgaggaa
    15361 gtatttgcgg atataggaga cgaacagtcg attgagcaaa gtctatcgac cttttcttct
    15421 catatgacga acactgtaga tattttatca aaagtcaatg aaaaaagtct ggtattattt
    15481 gacgagctcg gtgcaggaac agatccgcaa gaaggtgctg ctctagcaat cgcaatctta
    15541 gatgatcttg gtaaaaagtc tgcttatgtg atggcaacaa cgcattatcc tgaattgaaa
    15601 gtttatggat ataatcgggc aaatacgatt aatgccagca tggagtttga tgtggataca
    15661 ttaagtccaa cgtatcgttt gctgattggc gttcctggac gaagcaatgc gtttgaaatc
    15721 tctagccgat taggtcttga tacagaagtt attgatgaag cgaagcagtt gatgaatgat
    15781 gagagccaag atttaaacga aatgatcaca gatttagaga atcgccggaa aatggcagag
    15841 accgaatatc tggaaatgcg tcattttgtt tctgaggcac aagaacttca cgatgatttg
    15901 aaagaagctt acagctattt ctttgaagaa cgtgaaaaag aaatggaaaa agcaaagaaa
    15961 aaagcaaatg aagtcgtgtc agaggcggaa gaaaaagcag aaaaaatcat tgctgatatt
    16021 cgtaaaatgc aacagcagat tgggcaagga aatgtgaaag agcatcagtt gatcgatgca
    16081 aagacccaat tagcaaatct acatcaagaa gaaactttga agaaaaataa agtattgaag
    16141 aaggcgaaag aacaaaaaac gttgaaaccg ggagacgaag tgttggttac gacttacggc
    16201 caaagaggga cgcttttacg taaaaacggg aatcagtggc aagtagaaat cggtatatta
    16261 aaaatgaatg tgtccgaaga cgagctgacg cctgttgctc ctcaaaaaga gccgacacaa
    16321 cgagttatcc atgctgtacg atctgaatct agtagtcacg tgccgaatca attggacctt
    16381 cgaggaaaac gatacgaaga agcgctaagt gaggtagatc aatatttgga ttctgccatt
    16441 ctagctggtt atcctcaagt gactattgtc cacggaaaag gaacaggcgc acttcgcaag
    16501 ggaattactg attatcttaa aaaccatcgg agcgtcaaaa gctttgagtt tgcaccggca
    16561 aaccaaggcg gaaatggtgc gacaattgtt aaatttaagt aaagcttact aaaggtaagc
    16621 agatggttcg ctatccaagt atagaaatgc tatactagtc atagatttta aaaatggagg
    16681 tctttgatat gtcacaagta attacagata agacatttaa tgaagaaaca gataaaggac
    16741 ttgtattgat tgatttttgg gcaacttggt gtggtccatg tcgtatgcaa gcaccgattt
    16801 tggatcagtt ggaacaagaa tatgatgaag aagaattcag aattgctaaa atggatgtgg
    16861 atgaaaatcc agaaactcct caacaattcg gtatcatgag cattccaaca ttaatgttga
    16921 aaaaagatgg tcaagtagta gaaaaagctg ttggtgttca ttcaaaagaa caactacgtc
    16981 aaatgatcga tcaatattta taaaaactta cagcaaaaat aagctgaggg tgggactgaa
    17041 tccagttccg tcctcggttt ttttgcggtt gagttaaggt tattcaaatt aatatttttt
    17101 gagtataatt acgttgtcag gctggattgt ttttttgaca gacatgctat aataacttta
    17161 attgaatatg aaggggagta ccagatgaac gaacgaatca aaaacaaatt agctttactt
    17221 cctgatcagc ccggctgtta cttgatgaaa gataaaaatg gaacgatcat ttatgtcggc
    17281 aaagccaaaa tattaaaaaa tcgtgtccgc tcatatttta ctggtagcca taatacgaaa
    17341 acggaacggc ttgttagtga aatcgaagac tttgaataca tcgttacaga atcgaatatc
    17401 gaagcattgc tcttagagat caatctgata aagaaaaatg atcctaaata caacattatg
    17461 ttaaaggatg ataaaaccta tcctttcctt aagataacga atgaaaaata tccccgtttg
    17521 gtgattactc gtaaggtact aaaagacaaa gcactgtatt ttggaccata tcccgatgtc
    17581 ggtgcggcga atgagacaaa gaagatcctc gatcgattat ttcctctgag aaaatgcaaa
    17641 cctagtcaga cgaaggagcc ttgtctttac tatcatttgg gacaatgcct gtgtccttat
    17701 tatttcgacg tagatccgaa agtctatacg aaaatcgttg atgaagtgaa aaacttttta
    17761 aacggaggac acgaaaaaat cgagtcagag atccatcaga aaatggaaaa agctgctgaa
    17821 aacatggagt ttgaaaaagc tgcagaatac cgagaccaga tccgtgccat tgagacaatc
    17881 atgactcgcc aaaaaatgac aaatactgat ttactggatc gagatgtatt tggttatgcg
    17941 attgataaag gatggatgtg tgtccaagtg tttttcgtaa gacaaggaaa gctaattgaa
    18001 cgagatgttt caatgtttcc tttctataac gaagcagaag aagatttctt gacttacctc
    18061 ggtcagtttt atcaggaaaa tgagcatttc attcctaaag aagtcttgat tcctgatacg
    18121 atcgacaagc ccagtgtaga agctttgtta gctacgaaag tgctgcaacc aaaacgagga
    18181 gaaaagaaaa aattagttga attggcgagt aagaatgcct cagttgcttt gaatgaacgg
    18241 tttgatctga ttgccagaaa acaggaacgg acgatcggag cagttgaacg tttaggaaac
    18301 gcgatgaata tcccgacccc aatacgtatc gagtcttttg ataactctaa tatcatgggg
    18361 acgaatcctg tggctgcgat ggtcgtattc attgatggaa aaccggcaag aaaagaatat
    18421 cggaaatata aaataaaaac agtcaacgga ccggatgatt atgcatcaat gagagaagtc
    18481 atctatcgca gatattcgcg agtgattcgc gaagggttgc ctttacctga tttgatcttg
    18541 atcgatggag gaaaaggaca agtagatgtg gcaaaagatg tcttagaaaa tcaattagga
    18601 atcgatattc ctattgctgg catggtaaaa aacgataaac ataaaacaaa tgagttgcta
    18661 ttcggtacag atttggctgt agtaccgctt gagcgaaatt caccagagtt tttcttactc
    18721 cagcgaatcc aagatgaagt ccatcgattc gcaatcactt tccatcggca gctacgcagt
    18781 aaaaacagtt ttgcttcccg attagatgaa attgaagggt taggaccaaa acgaaaaaaa
    18841 atgctgctta aagagttcaa atcgttgaaa aatatcacag cagccacgac agaagaatta
    18901 caagaaatcg gactgccgaa aaatgtcgca caaaacgtat ttgataaatt acatcaggaa
    18961 tgaggaaagg gcactttaca aagaaagtaa agtgtcctat cattttatgt acaacaaaaa
    19021 gagcgtttac gttttgttgg taaataataa atcgcttacg tctggtgtct aaggatatat
    19081 gtgatttctt gaagaaggag tgaagaaatg ttagaagtca aggatcttgt taaaactttt
    19141 gggaactaca cagctgtcga tcatgtctct ttccaaatcc ccgatggaaa aatcatgggg
    19201 ctaattggcc aaaacggggc tgggaagaca acgacttttc gtttgatctt ggattttttg
    19261 acacaagacc aaggagaagt gttatggaat ggccatcatc ttggggagaa agattatgat
    19321 gtcatcggtt atcttccgga ggaacgggga ctttatccga aagtaacgat tcaagaccaa
    19381 ctgctttatt ttgcagaact tagagggaaa tcaagaaaag agattgaacc aaaaatcgac
    19441 ttttggatgg aaaaatttca ggtaaaaggg aaaaagacgg ataaagtaaa atctttgtct
    19501 aaaggaaatc agcaaaaagt ccagctgatc gccacgttaa tccatgaacc taaattgatc
    19561 attttggatg agccattcag cgggttagat ccggtaaatg ctgaattgct aaaagatgga
    19621 atattggaat tgaaaaaaca tggttcatgt gtcatttttt ctagccacaa tatggataat
    19681 gtagaaaaaa tctgcgatca tttgatcatg ttgcgaaacg gaaaaatggt tttgaatgga
    19741 aaagtacatg agattcgtga atcatttggc cgtacgaagt tattcttgga atctggttta
    19801 agccaacagg aagtagaaga aatcgaaggc gtgaaaaaag tgatccttca tgaagacaat
    19861 acgttagaga ttaccttaga agatcctgaa gctgggaaag aaatctttac tcgtgcgact
    19921 cagttcggct acattcctat gtttaaccag caaccgccaa ctttggaaga gattttcaaa
    19981 ttgaaagcag gtgaaatcga tgcgtaagtt ttggattatt accaaagatg tctatttaaa
    20041 aaatgttaag tcgatttctt tcttgatcat gatattagtc ccttttatca tgctgggtat
    20101 catttatgta gcaggaaact ttgctcaaca aaatagtgat gtcaataaaa tcggcgtatt
    20161 ggctgataat gagcaaatca gtcagcaaat gagccaagtc aaaacagatg attttgagtt
    20221 caaagcagtt gattctgaag aaaaagcaca aaaagagctt tcggaagaaa aaatcgatgc
    20281 gtatttagta gtctcaactg agaatgataa gataaatggg atgctttata gcgaaaattc
    20341 attaggacaa agtacacaac tactgataca acaacaactg actggcttgc aatcgatgct
    20401 tcgagcgagt caattaggca tttcaccaga agaagtagct agcttgagcc aacaagcaag
    20461 tttgactcga caaaaagtca attttgattc aaaaggaaaa atgacagtcg gagaagataa
    20521 ctcgacagtc caatacgtcg taagttatgt agcaacgatt gtcttgttca ttattatttt
    20581 gacgtatgcg cagatcattg cacaagagat cgcttctgaa aaaggcacgc ggatcatgga
    20641 agtgatcctg tccagtacga ctgcacaaaa gcatttttac ggaaaattaa caggtgtttt
    20701 acttgttgca ttaacgcaaa tggcattata cggtattatt tttggtatag gatacagtca
    20761 attcaaagat atggaaatgg tcaaaaatat attagacggt atctcattgg ataacttgat
    20821 tggaccattc ttgtggtatt ctcttctatt catgatcttt ggcattttga ttttttctgt
    20881 tttagcagct ttatgtggct ctttggtcaa caaagcagaa gatacagcaa aagcaatttt
    20941 gccagtgact tatctatctc taggcggata catgcttgga ttgatactag gagcttcaga
    21001 tccaaacaat atcgttattc ggatcacttc gtatattcca ttcttatctt catatattat
    21061 gccgattcgc ttagcaaatg agacagtcgg tatgggtgga gcattgattt ccttgttggt
    21121 acttgtagcc gtaacattct tattgatgat cttgtctgca aacatgtaca aatcgaatgt
    21181 actcgtttat agcgaaggcg gattatggag ttcattgaaa caatcgattt caattatgag
    21241 gaatgaacga aaaaaagcat aaaacaatcc taaaatacag aaggaagctg gacatctatt
    21301 gttcccagct tctttttgta tagacctatt ttatttattt tactatattg gatatatatt
    21361 cattgaaaat agtgtataac aactttataa tattgagtat accaattttt ggaggcgttt
    21421 taatggaccg ttatttagat ttactttcag aaaaattccc ctcaacggag gatgtcatca
    21481 cggaaatcat caatctcgaa gcaattttgc agcttccaaa aggaacagag ttatttttga
    21541 gtgatataca tggcgaattt cctgcttttg atcatattct gcgtatcggt tcaggaaatc
    21601 ttaaagaaaa gatcaaagac cttttttctg aacaaatgac ggaaacggat atcagccaat
    21661 tcactatttt tacggcttat ccagaatatg cattaacgac tgattggtac aaagaacaag
    21721 ataaatcgca gctgatccat catttgatcg atcttttacg gtttacgaca gtcaaatata
    21781 cccggtcaaa agttcgaaaa ggattgccga aagaatatag ctatatcatt gaggaactct
    21841 tgtatatcga tgaccgtatc aatggaaaaa aagactatgt aaaaaaaatc attgaacaac
    21901 ttgttttgat gaaggaagaa gaaaaatttt taacgaaact tgcacagacg atccaaaaat
    21961 ctgtcatcga tcatttgcat atcgttggag atatttttga ccgaggaact caagcagcca
    22021 aagtgatgga tcgaatcatg gatttttctt ctgtagatat ccaatgggga aatcatgata
    22081 ttttgtggat tggtgcgtat tgcggctctg aggcatgttt actgacactt ttacggattg
    22141 cagcacgcta taattattta tttgaattag agaaagaata tgggttgaat ttacgtccat
    22201 tgttttcttt tgctcatcaa acttatcaga agaatccagt atttgcgcct aaaaacagta
    22261 aaaatctatc cgaaagagag caagaagaat tagaaaaaat ccatcaggca ctttctatcc
    22321 ttcaattcaa atcagaacat cagttattgg atcgtcgacc tgaatttaaa atggacgatc
    22381 gtaaattgct ggaaaaaatc gactatgaag catcaacgat tgatttagaa ggtcagctct
    22441 atgagttgaa aggaacttgt tttcaaacga tccagccaga tgatccaaaa aaattcttgg
    22501 aagaggaaca aaaagtgatt gatagtttga tgtattcatt ccagcactct ttgagaatgc
    22561 aaaaacatat gacgtttttg atcgaaaaag ggggaatgta tctcacgaac aatcagcatg
    22621 tgctgtttca tggctgtata cctgtagacg agaagggaca gctcttagca tttgaattag
    22681 accaatctta tcgcggaaaa gaactgctgg gcttttttga aaaacagata actgaaagcg
    22741 caaaagatct gactgcaaaa gaagatttag caaccgattt gatttggtat gcgtggagtg
    22801 ggccattttc gcctttattc ggcaagagta aaatggcgac ttttgaacgt tattttctca
    22861 cagagcctca tacgcacgtc gagaaaagca atccttatta tcacttgcga gatgaagagt
    22921 ggttcagtga aaaaatttta gcagaattcg acacaaaaga agaagggtca gcaatcatta
    22981 atgggcacac gccagtaaaa gtaaaaaaag gggagtcacc gatcaaagca aatggaaaag
    23041 tatttgtcat tgacggaggt ttatccaaag cttatcaaaa aacgacagga attgccggtt
    23101 attcactatt atgcaattct tatggtttcc aaatcgtgac acattatccg tttttgccta
    23161 ttgaacaaca atttgcaaaa aaatccgacc aaacaaatgt gaaaaaagta atcgaacagc
    23221 aactaccacg caaactcaat cgtgatacaa caaaaggtat ggaactgcag cagcagatta
    23281 cccagctcaa aaggctgttg gactatcgaa aggacgattg agaagaattc cagtgaacat
    23341 tgttccatag actggagact gagacaaaag tcgtgtcgca gtctcttttt ccggataaac
    23401 ggtgttcttc tgccaactcc tcggtcacaa gtcacactat tcaacaaaca tgaaaagctg
    23461 ttttttgaaa ttgctccttg tgactcagag ttgaacggca ggctcacgac ctcttggtat
    23521 aaacggtgtt caaaagccag tttctcggta ttaagccaaa tttatgcaaa aatgtgaaaa
    23581 gccattttca caaaattttt cttaatactc gaaactaaac agctttttca caaccccctc
    23641 agaaagtcta ttaaaattca ttcacaaaga aatcgaataa gcgctgttca gatggatgta
    23701 cacggtgggt aagttccgga tgccattgaa caccgaggat tttatgcata tcatctttgc
    23761 tttgaaccgc ttcaacgagt ccatcgttag aaaaagcgat ggcttttaat aaaggtgaaa
    23821 gatctttgac tgcctgatga tggtaagaat tcacaaaata tggatcgggg agaaggttgc
    23881 ttaacaaact gtctgaaaca atcttgactt cgtgagtcgt aaattgcggg gcagtgggct
    23941 gttgttcatg ttttactgac cactttggat ataatgaaag atcttgataa agtgtacctt
    24001 ccaaaacgac attcaatagt tggagtcccc ggcagactgc aaaaattggt tttctttgtt
    24061 tcaatgcttc caagatcagt gcctgttcaa attggtctcg ttgaatattt gtttccgtta
    24121 gctttggatg aggatcttcc atgtaaagtt gtggactgac atcttgacca cctgcaagaa
    24181 taagtttgtc gatttgtcca atcagttgag gagctgaatc tggagacatg actggcagaa
    24241 taagaggcaa tccttgagca tctaaaactg catccacgaa tccttgcggt gtatagctca
    24301 cttgatttcc ttggaaagta tctgttgctc gaatcagttg atttccggca atgccgatga
    24361 ttggtttcat ttgtattccc cctaaagtag acttattgta acaaggattt aaaaaaaatt
    24421 aaactatgta agacttgccc ctatacttat ctgttcttgc tgtgttacta tctgtaaaga
    24481 gaaatttcgt gtgaaattca tgacgatagt tgaaaatatt gcttaatttg ttataataaa
    24541 aagtaagcag aaataaaaag gttgggcgta agcttcaagg atctagttct tcaaggggtg
    24601 agaaggacat tttattgaag gggcctgcct atactaagtt atctttagag gtacatactt
    24661 tcaatgaaaa aagaaagcat gtacctcttt tttaaatcga aaggaagtga agcaagtgtt
    24721 agaagctttg gaacaaatcc gaaaagcaga agaagaaaat gaaaagcgtg ctcaaacact
    24781 gcggcagcag ctgaagatct atgcaacaga aaaagagcag gaattggaaa cgatccaaac
    24841 aaactatcga caacaactaa aacagttggt ggaagaaaaa gaacaatttg aaatacaaga
    24901 attagaaaaa gaaaaaatga aattgaaagc agaagcagac aaggtgcaag caactttgaa
    24961 agatcaatat caaaagcatc agagtcaagc gatcggtgcg atcattgaaa gggtgaaaga
    25021 aacttatggc cgtcactaag atggaaaaag tgaccctcat ttcagataaa agaaatcagg
    25081 agaggatttt acaagcagta caaggaatcc aaaacgttga aatccgggat ctgtatcaaa
    25141 atagaacaaa caaccagtgg gtggagaagt attttccaga taccacgatt attgataaag
    25201 aagcaaaatt aagcgcttta ggtaatcggt tgaaagacat ccaagaagct atccaattca
    25261 tacaacacca cggcgatagc cgtcaaaaga aactccattt gaaacgtcaa gaactttcct
    25321 tacaagattt ggaagccaat tattccgaag aaaatttcat aaataaattg aaagaagtcc
    25381 ttgatttaaa agaaaagtgg gaacaactaa ctgaagaacg tgatcgttta gaagaagagg
    25441 aagaatggct tcttcattgg caacagctgg atattgctcc aagtatgtat gcaagtaaga
    25501 cgaccatttt tgagatgggg actgtgagtg cagcaaattt tgaaagtttc aaggaagaac
    25561 tgagtaagct tgatgacgtt tatatggaag aaatagatta tacgccaaaa caagtttatc
    25621 tttcgtatgt tgctttaaaa aaatcagttg ctgttataga agaaattgct gggaaatacg
    25681 ggttcaataa agaaacgtat ccttatgaac aaaatcctaa agaaaaactg caagagacca
    25741 agcagcaatt atccattgtt cacgagcagc aaaagaagat aacgacagcg ttaggtcagt
    25801 gcagtggcta tatccaagaa tttgaatggg tagaagaagt cacacttgcc gttgcagaac
    25861 gagaaaaaat aaaagaacgg tttatccatg cgacttatct gatcgttttg caaggttggg
    25921 tagatgcaga agaaaaacag aatttgctgc atgtgttaaa cgaaaccgta tcagaagaag
    25981 atatatacgt cagtttcgaa acaccaaact cagaagagat tcagcaggaa gtccctacta
    26041 aattgaaaaa ccatccgctt gtcgaacctt ttgaattgct gactgaaatg tacagtctgc
    26101 caaaatatga agaagtagat ccaacacctt ggatgacacc attttatctt gtcttcttcg
    26161 ggatgatggt cgcagatgtt ggttatggtt tgctgatgct cattgggaca atactagctc
    26221 aaaaactact tgttctacca cgcgggatga aacgtttcgt aaaattcttt gagattctct
    26281 cgataccatc gatcatctgg ggattgatct acagttcatt tttcggacag tcattaccaa
    26341 aagaactgtt tggcatccgc ttgcctttcc caatcctatc tacgacggat gacgtcaata
    26401 cgattttgat tttatccgtg attttcggtc tgatacagat tttggttgga ttatttgtgg
    26461 cagcaaagga aaatatcagg agaaaagatt acttgagtgc tgtcagtgat ggatttgcct
    26521 ggcaagggat cctaattggg atcgttattg cgttggttgg tgccatgttg ataaaaaata
    26581 cagcatttgt ttaccttggc gctgggctat cgatcattgc tgccttatgt atcttgatcg
    26641 ttccgattat ccaaacgccg tctaaagtca aaggtgctgc caaaggtgcg tataatcttt
    26701 acggactgac cggctatatc ggtgatttag tcagttatac acgtttgatg gcattgggta
    26761 tttcaggcgg aagtattgga gcggcattca atatgctagt tgctttcatg ccgccagctg
    26821 ctcgattcag tgtagggatc cttttgatca tcgctttaca cgcactgaat atgtttttga
    26881 cactgctaag tgcctatgtc catggcgcac gattgcagta tgtcgaattt tttgggaagt
    26941 tttatacagg aggcggacgt gcctttgatc cgctgaaaac ggcggaaaaa tacgtcaaca
    27001 tcaatcacaa gaaaaaaaac aataaataat tggaggattt ttatataatg atggattact
    27061 taattagtca aaatggtgga atggtatttg cggtattagc gatggcaaca gcgacgattt
    27121 tttcagggat cggttcagct aaaggtgttg gaatgacagg tgaggcagca gcagcattga
    27181 caacaagtca acccgaaaaa ttcgggcagg ctctgattct gcaattgctt ccaggcacgc
    27241 aaggtttgta tggattcgtt attgcctttt tgatttttat caacttagga aatgacatgt
    27301 cagttgtaca aggactgaac tttctaggtg cttctctgcc gattgctttt acaggcttgt
    27361 tttcaggtat tgcccaagga aaagtcgcag cggcaggtat tcaaatttta gcgaaaaaac
    27421 ctgaacatgc tacaaaagga atcatttttg ctgcgatggt tgaaacatac gcgattttag
    27481 gtttcgttat ttccttccta ttagtgttga atgcctaagc ataaaggagg aaaagcagat
    27541 gaacgccatt gaaaaaatca tctctcagat gaacgaagca gcagagcagg aaagagcggc
    27601 gttagaacaa gaagagcgaa tgaagatcga tcaaaacttt gaacaaaaac gtacgcaagt
    27661 tgaaacagaa catcaaaaac aaaaagaaaa acaaatcgaa ttactcgaaa aaaaataccg
    27721 tcagctaagg aatcgccagc aagtagaagt acgacaagaa aatttgaatg cgaagcagga
    27781 atttttaaga cgattgtttg cagatgcggt aactgaaatg gaaaattggg atgaatctga
    27841 acaaatccag tttatcaaaa atgcattgta ttcattacct ttgacaggaa aagtagcatt
    27901 cattgctgga gaaaaatcag cagcctatct ttctcagacg ttgttggatg aatggaacaa
    27961 tgaacttccc tttatgatgg ttttaagtga tgaaactgtt gctgatcaag cgggttttct
    28021 gataaacgat caaggagttc agtataattt tcttttcagc agccttgtac aagatatcca
    28081 aggaacgatg agttttgaga ttgccaatca attattcgaa taaggaggga tcagtgtatg
    28141 gaataccacg aactaaatcc cttgattcgc ggaagagagc ttgaactttt gtccaatgag
    28201 acatttgaac ggatgattca aacaaattct gtcgaagcac ttggtgaaat attgaaatcc
    28261 acgatttaca gtccatatat ttatgacgga tatgaatatg acttcgaaca gaatctttta
    28321 aaggagcaga gccgtctatt tcgctggtta aaggaatcag caccggagcc tgaaatcgtt
    28381 tggatctata cgatgcgtta tactttccat aatctgaagg ttttgacaaa agctgagatg
    28441 acaaatcaaa atctggacca tttgtatatc gacgatggat tttattcatt agaaaccttg
    28501 aaggatgcca ttcatacgca agtttcaaat gaacttcctg aaagcattat ggacagtata
    28561 cgagaagtac gtgattattt tgaagagtct accattcttc agggaattga cgtgatttat
    28621 gatcgacagt ttttgacaga gcagcgacgg cttgccgata agctaggata tcaagaattg
    28681 ctgacagaag tcattgcttt tattgacttg accaatatca caacgatggc tcgaggaata
    28741 ttgcagaaaa gaacatatgg gttcatgacg acggtacttt ccagctcggg aagtatcgaa
    28801 aaagaagtat ttttgaactt tgtagataaa gatatggaag attacacgaa ttttctatta
    28861 gagacagatt atgctgattt tctgcgccct gtcatccgtg agggaaatat tgatctcgta
    28921 aagcttgaac agttgaaaga tgattatcta agttcattgt atcaagaagc acaaacccag
    28981 gctttcggac cactgccact actagccttt ttgaacgcaa aagaggtaga aagcaaaaac
    29041 cttcgtttac tggttatcgg caaaagaagc catttcgaat cagaaacaat cagaaagcgg
    29101 gtgagacaag tctatgacgc ataagatcgg tgtagttggc gacaaagatt ctgttttgcc
    29161 attcaaattg tttggctttg atgttcgtta tggtacaacc aagcaaaccg ttcgtcaggc
    29221 aattgaagaa atggcaaaga atgagtatgg cgtgatttat gtcacagagc aatgtgcagc
    29281 attgagccca gaaacgatcg aacgttacaa ggaacaaatg atacctgcta tcgtattgat
    29341 tcccaatcat caaggatcat tagggatagg gctggaagaa attcaaaaaa acgtagaaaa
    29401 agcagtaggt caaaatatct tatagtatga aaaggagata gaaattttgc aaattggaaa
    29461 aatcataaaa gtctccggac ctcttgtaat ggctgagaac atgtctgatg ccagtatcca
    29521 agacatgtgt ctagtgggag atctaggagt tatcggagaa attatcgaga tgcgtggcga
    29581 tgtggcgtct attcaagtat atgaagagac ttctggaatt ggtccaggcg aaccggttcg
    29641 ctctactggt gaagctttat ctgttgaatt aggaccaggg atcatttcac aaatgtttga
    29701 cggaatccaa cggccattag atacctttat ggaaatcaca caaagtaatt tcttagggcg
    29761 cggagttcaa ttgcccgcat tggaccatga gaaaaaatgg tggttcgaac caacagtaga
    29821 agccggagaa acggtgagtg ctggagatgt tatcgggatc gtagaagaaa caaaagttat
    29881 aaaacataaa atcatggtgc caaatgggat aaaaggaacg atcaaaacaa tcgaaaaagg
    29941 ttcattcact attgatgaaa cagtatgtgt cattgaaacc gaaaatggtg acaaaaattt
    30001 gacgatgctt caaaaatggc cagtaagacg tagtcggcca atcaaagaaa aattaaaccc
    30061 ggatgcaccc atgatcactg gacagcgagt gattgataca ttcttcccag tgacaaaagg
    30121 aggagcagca gctgtacctg gtccttttgg tgcagggaaa acagtcgttc agcatcaaat
    30181 tgcaaaatgg gccgatgtgg acatggttgt ctatgtggga tgtggcgagc gtggaaatga
    30241 gatgacggat gttttaaatg aattccctga attgattgac cctaatacag gagaatcgtt
    30301 gatggaacgt acggtattga ttgctaatac atcaaacatg ccagtagcag ctcgagaagc
    30361 ttctatctat acgggaatca caattgcgga atatttccgt gacatgggtt atgacgttgc
    30421 tatcatggca gactccactt cacgatgggc agaagcactt cgtgagatga gcggacgttt
    30481 ggaagaaatg cctggtgatg aaggttatcc agcttatctt ggttcacggc ttgcggaata
    30541 ctatgaacga tctggaagag tcattgcatt aggatcagaa ggtcgcgaag gaagtataac
    30601 cgcaatcagt gctgtatccc cgtctggagg ggatatttcg gaaccagtca cacaaaatac
    30661 attgcgggtc gtaaaagtat tctggggtct tgattccagt ttagcccaaa aaagacattt
    30721 tccttcgatc gattggatac aaagctattc tctttattca acagaagtag ggaagtatat
    30781 ggatcatatc cttcaagctg actgggcatc catggtcacc gagggcatgc gtattttgca
    30841 ggaagaacag cagctgaatg aaatcgtgcg tttggtaggg attgattccc tttcagataa
    30901 tgaccgtctg acattagaag tggcaaaatc cattcgtgaa gattatttgc agcaaaatgc
    30961 ttttgatgat gtcgatacat tcacttcaag agagaaacaa ttcaaaatgt tgaagatgat
    31021 cttgaccttt ggtgaagaag gacgccgtgc gttgtcgtta ggtgcttatt taagtgagat
    31081 catggacggt accgtatcga ttcgtgaaag gatcggacga agcaaatata tcccggaaac
    31141 agaattagaa aaactagatc aaattattgg tgaaatcaag caaacgatgc aaacgatcgt
    31201 ttcagaagga ggcatgagca atgattaaag aatatcgtac gatcggtgaa gtcgtcggtc
    31261 cattgatggc ggttgaaaaa gttgctggtg tcaaatatga agaattaatc gaagtccgta
    31321 tgcaaaatgg cgagattcgt cgtggacaag tattggaagt ccaagaagat aaagcaatgg
    31381 tccaaatatt cgaaggaaca agtggtatca atttacgtga ctcttctgta cgatttttag
    31441 gccatccttt agaactaggt gtatcggaag acatgatcgg tcgagtattc gatggtttgg
    31501 gacgaccgaa agataacgga ccagagatcc tgcctgaaaa gcatttggat attaacggag
    31561 aagtaatcaa tccagtagct cgggattatc cagatgagtt tatccaaaca ggtatctctg
    31621 caatcgatca tttgaataca ttagttcgtg ggcaaaaatt accagtattt tctggctctg
    31681 gtcttcctca taaagaatta gctgctcaga tcgcgcgaca agcaacggta ctcgattcat
    31741 cagatgattt cgccgtagtc tttgctgcta ttggaatcac ttttgaagaa gctgaatttt
    31801 tcatggaaga ttttcgtcaa acgggcgcaa tcgaccgttc tgtcatgttt atgaatttag
    31861 caaacgaccc tgcaatcgaa cgtatcgcta ctcctcgtat ggctttgact gctgcagaat
    31921 atcttgctta tgaaaaggga atgcatgttt tggttatcat gacagacatg accaactatg
    31981 cagaggcatt gcgagagatt tctgcagcac ggcgagaagt tccaggaaga agaggatatc
    32041 ctgggtatct ttacacgaac ttagctactt tattcgaacg agcaggaaga atccgcggac
    32101 taaaaggatc ggttactcaa attccgattc taacaatgcc agaagacgat aaaactcatc
    32161 caatccctga cttgactggt tatattacag aaggacagat catcttgact agagagcttt
    32221 acaagagtgg gattcagcca ccaattgatg tactcccgtc tctttcgcgg ttaaaagata
    32281 agggaactgg acccggaaag acaagagaag accatgcagc tacgatgaat cagctttttg
    32341 cagcctatgc acaaggaaaa caggcaaaag aattagccgt agttttaggt gaatctgctt
    32401 tatctgatgt agacaagatc tatgcgaagt ttgctgagcg gtttgaaaaa gaatatgtca
    32461 atcaaggatt ctacacgaac cgttcgatta ctgaaacact tgatctaggg tgggaactgc
    32521 tatcgatgtt gccaagaacc gaattaaaac gaatcaaaga cgacatgctg gacaaatatc
    32581 tgccagaagg gaagtgagtt ctgatggcac gattgaatgt gaatccgacc cgcatggaat
    32641 tgactcgatt gaaaaaacag ttgacaactg ccacaagagg gcataaatta ttaaaagata
    32701 agcaagatga actgatgcgt caattcatcc tgttgattcg aaagaacaac cagctgcgtc
    32761 aagaaatgga agagtctatg acagaagcaa tgtctgattt tgtattggca aatgcttcga
    32821 tcacggaacc tttcatcgaa gaattatttg ttttgccggc ggaaaacgtt gatttgtcta
    32881 tcgtagacaa aaatatcatg agtgtaaaag tacctgtgat gaattttgaa tatgatgaac
    32941 agctgacaga cacacctttg gaatacgggt atttgaattc cagtgccgaa ctggatcggt
    33001 caatcgatcg gtttactacc ctgcttccaa aacttttgga actgacagaa atcgaaaaaa
    33061 cttgtcaact gatggcgagt gaaatcgaaa agacacgtcg gcgagtcaat gcattagaat
    33121 atatgacgat ccctcaatta gaagaaacga tctattatat tcgtatgaag ctggaagaaa
    33181 acgagcgcgc agaagtaaca aggttgatca aagtaaaaaa tatgggtacc actgactaag
    33241 tggtatcttt ttttaaaaga ggaaaacgat gattaaacaa agcaaacaaa taaagaaacg
    33301 tttgccggct gttcagcttc tcgcattagg attttttctc ctgattttag ttggcggcgg
    33361 cctacttacc ctaccatttt ttagtaatag tgggcatggg acaaatttta tcgatgcgct
    33421 tttcactgca acttctgctg tctgtgtaac gggattgaca acattgaata cagcagagca
    33481 ttggaacagt gctggtcaat ttttgatcat gcttttgatt gagatcggcg gtcttggttt
    33541 tatgatggtt ccgattctat gctttgcatt gataaagaag aaagtcagtc tcagcacacg
    33601 tattatatta aaagaagcgc tgaatctaga agaagtatca ggtgtgatga agctcatgct
    33661 ttacatccta aaattggcct ttgtgatcca attgattggt acggttgctt tatcttttgt
    33721 atttgtgcct gaatttggct ggttgaaagg tatctggtac agtttgttcc atgcaatctc
    33781 tagtttttgt aatgcagggt ttgatttgtt aggagacagt ttagccaatc accagcaaaa
    33841 tgtttatttg atcatggtgg tatctgcttt gatcatttct ggtgggttag gcttcatcgt
    33901 atggcgtgat ttactggaat atcatcagaa aaagaagatg acgattcatt caaaaatcgc
    33961 attaaccgtt acaggtaccc ttttattagg cggattcgtt gtgttctttt tcacagagcg
    34021 caatggggca acgctagtgg aagggaatat gtttcaacga ttggcaaaca ccttttttat
    34081 gagtgtgaca cctagaacgg ctggatatta ttcagttgat tacctccata tgagccatgc
    34141 aggtctgatt ttaacgatgt ttttgatgta catcggcgga acatctgggt cgacggccgg
    34201 cggattgaaa acaactacgt taggtgtgtt gctgatccag atgcgttcga tgttgaaagg
    34261 acgtacgaga gcggaagcat ttggccgtac catccgacaa ggcgcagtgt tgcgggcact
    34321 tactttattt tttgttacgt tgacgttgtg tgtagtcgcc atcatgattt tatcggtgac
    34381 tgaaacgata cctaaaactt ctggcatcga atatattgct tttgaggtct tctctgcgtt
    34441 tggtacagta ggtcttacga tgggattgac gccagacttg actgcaatag ggaaattgat
    34501 cattatagcg cttatgtaca ttggacgagt tggtgtattg actgtggtct tctctctgat
    34561 gacaaaagca caaagaacac aatcaaaata caaatatcca gaagaaagtg tcatgattgg
    34621 ataatttcat cttctgacag aaacctagtg atttagtaaa ataagaaaat aaaataattt
    34681 ttcttgcttc ttggatactt taagtgcaaa atgttcgttt at
//
LOCUS       DACDUU010000025        34353 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.025, whole genome shotgun sequence.
ACCESSION   DACDUU010000025 DACDUU010000000
VERSION     DACDUU010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.025"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(133..306)
                     /locus_tag="IWL30_001231"
     CDS             complement(133..306)
                     /locus_tag="IWL30_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9706009.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            754..3116
                     /locus_tag="IWL30_001232"
                     /pseudo
     CDS             754..3116
                     /locus_tag="IWL30_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            3484..4407
                     /gene="truB"
                     /locus_tag="IWL30_001233"
     CDS             3484..4407
                     /gene="truB"
                     /locus_tag="IWL30_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAP9706010.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            4411..5352
                     /gene="ribF"
                     /locus_tag="IWL30_001234"
     CDS             4411..5352
                     /gene="ribF"
                     /locus_tag="IWL30_001234"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAP9706011.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            5704..6489
                     /locus_tag="IWL30_001235"
     CDS             5704..6489
                     /locus_tag="IWL30_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9706012.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            6502..7353
                     /locus_tag="IWL30_001236"
     CDS             6502..7353
                     /locus_tag="IWL30_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAP9706013.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(7477..7929)
                     /locus_tag="IWL30_001237"
     CDS             complement(7477..7929)
                     /locus_tag="IWL30_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9706014.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(8394..8762)
                     /locus_tag="IWL30_001238"
     CDS             complement(8394..8762)
                     /locus_tag="IWL30_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAP9706015.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            8912..9394
                     /locus_tag="IWL30_001239"
     CDS             8912..9394
                     /locus_tag="IWL30_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAP9706016.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            9431..10441
                     /locus_tag="IWL30_001240"
     CDS             9431..10441
                     /locus_tag="IWL30_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAP9706017.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(10556..11269)
                     /locus_tag="IWL30_001241"
     CDS             complement(10556..11269)
                     /locus_tag="IWL30_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706018.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(11459..12049)
                     /locus_tag="IWL30_001242"
     CDS             complement(11459..12049)
                     /locus_tag="IWL30_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAP9706019.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            12533..12760
                     /locus_tag="IWL30_001243"
     CDS             12533..12760
                     /locus_tag="IWL30_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAP9706020.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            12776..13942
                     /locus_tag="IWL30_001244"
     CDS             12776..13942
                     /locus_tag="IWL30_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAP9706021.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            14176..15219
                     /gene="hrcA"
                     /locus_tag="IWL30_001245"
     CDS             14176..15219
                     /gene="hrcA"
                     /locus_tag="IWL30_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAP9706022.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            15313..15876
                     /gene="grpE"
                     /locus_tag="IWL30_001246"
     CDS             15313..15876
                     /gene="grpE"
                     /locus_tag="IWL30_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAP9706023.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            15927..17756
                     /gene="dnaK"
                     /locus_tag="IWL30_001247"
     CDS             15927..17756
                     /gene="dnaK"
                     /locus_tag="IWL30_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAP9706024.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            17907..19073
                     /gene="dnaJ"
                     /locus_tag="IWL30_001248"
     CDS             17907..19073
                     /gene="dnaJ"
                     /locus_tag="IWL30_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP9706025.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(19155..19820)
                     /locus_tag="IWL30_001249"
     CDS             complement(19155..19820)
                     /locus_tag="IWL30_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAP9706026.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            19796..19969
                     /locus_tag="IWL30_001250"
     CDS             19796..19969
                     /locus_tag="IWL30_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9706027.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(20014..20751)
                     /locus_tag="IWL30_001251"
     CDS             complement(20014..20751)
                     /locus_tag="IWL30_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9706028.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(20753..22255)
                     /locus_tag="IWL30_001252"
     CDS             complement(20753..22255)
                     /locus_tag="IWL30_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9706029.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            22514..24508
                     /gene="uvrB"
                     /locus_tag="IWL30_001253"
     CDS             22514..24508
                     /gene="uvrB"
                     /locus_tag="IWL30_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAP9706030.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            24518..27334
                     /gene="uvrA"
                     /locus_tag="IWL30_001254"
     CDS             24518..27334
                     /gene="uvrA"
                     /locus_tag="IWL30_001254"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAP9706031.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            27597..28862
                     /locus_tag="IWL30_001255"
     CDS             27597..28862
                     /locus_tag="IWL30_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAP9706032.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            28866..29597
                     /locus_tag="IWL30_001256"
     CDS             28866..29597
                     /locus_tag="IWL30_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAP9706033.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            29732..30616
                     /gene="rapZ"
                     /locus_tag="IWL30_001257"
     CDS             29732..30616
                     /gene="rapZ"
                     /locus_tag="IWL30_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAP9706034.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            30613..31611
                     /locus_tag="IWL30_001258"
     CDS             30613..31611
                     /locus_tag="IWL30_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAP9706035.1"
                     /translation="MKMKTYRIRKPKVVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            31645..32580
                     /gene="whiA"
                     /locus_tag="IWL30_001259"
     CDS             31645..32580
                     /gene="whiA"
                     /locus_tag="IWL30_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAP9706036.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            33123..33773
                     /locus_tag="IWL30_001260"
     CDS             33123..33773
                     /locus_tag="IWL30_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706037.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            33960..>34353
                     /locus_tag="IWL30_001261"
     CDS             33960..>34353
                     /locus_tag="IWL30_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAP9706038.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIK"
BASE COUNT        11394 a         5818 c         7296 g         9845 t
ORIGIN      
        1 aaatcagggg ttgcgggatt tttttgtttt cattaagacg tattacctaa gagttccatg
       61 actttagtct tgcggcgtgt cagctaacca aaaaagagaa aaagaaaagt aaaaaattcg
      121 actttctttt ttctagtagt atataaattt ttgcatatag ataaagatca agataccgat
      181 aacggaagct agattgtaat ctattttagt gacagtcagg tatagccaag taactagaag
      241 atcagtggaa cctgcaccat aaaagtttga gtttgtcggt ccgttgacag ttaaaagaaa
      301 aatcacgttg aagttattga tatttccgat tacgccgctt cgatttggtc acgatccaaa
      361 ttttggataa tccctgtaga aattagcata gtagctggga taccaattca catattgaca
      421 aagataacag agacttttac ataaaacgga tcaatcagga aaggaattgc tcgatccagc
      481 cccagttttc cagcaatgtg ttaactggcc cagcgtcatt cagcaaatta tgcatgatga
      541 ggagcgaaat gaactgcgga acagccattg tgataacgac aagttttttg gatcaatgta
      601 aaaaacgtca gcgaaaggaa aaatttcgtg gttttttata cctgttcatt tggctagata
      661 taaaatccaa acaaatgaac agaagtgctt attaggtttc aattgggagt atctgcgcta
      721 gaataagagt aataatgaaa aggagtgtta tgtatggatt attctagtaa agaatacttt
      781 gataaaatga cggcttggtg gcgtgctgct aattatcttt ctgttgggca actttatctt
      841 aaagataacc ccttactgag acgtacactg aaacccgaag atgtcaaaaa acatccaatc
      901 ggtcattggg gaacgattcc tggccaaaat tttatttatg tccatttgaa ccgagtcatc
      961 aataaatatg atttgaacat gttttatatt gaagggccag gacacggcgg acaagtcatg
     1021 gtatcgaatg cttatttaga tggaagttac acagagatct atccagaagt aacggaagat
     1081 gagacaggaa tgcaaaaatt attcaaacga ttttcatttc ctggaggcat tgcttctcat
     1141 gcagcaccag aaacaccagg ttctatccat gaaggcggag agcttggata ctctctttca
     1201 catgcagtcg gagctgtatt agacaatcct gaagtaatct ctgctgtcgt gattggtgat
     1261 ggtgaagcag aaaccggtcc tttagctgga agctggttct caaatgtctt tatcaatcca
     1321 gtgatagatg gagctgtttt accgatcctt catttaaatg gtgcaaaaat agccaaccca
     1381 acgatcttgg cacgtaaatc agatggagaa ttagcaaatt actttaacgg acttggctgg
     1441 gaacctttct ttattgaagg aaatgattca gaaaaattaa atcctgttat ggctgaaaaa
     1501 atggatcaag caatcgagaa aattaaatcc atccaaaaag aagctcgcct gaagacagca
     1561 acagatgtcg tgatgccaaa atggccagta ttgatcgtac gcacaccaaa aggatggacc
     1621 ggtccaaaag aatgggacgg ggaaccaatt gaaggaacct tccgagcaca ccaagtaccg
     1681 attcctgtag atcaagaaca tatggatcat gcagatgctt tactccgttg gctgaaatcc
     1741 tatgaaccag aaaaattatt tgatgcacaa ggacgtattt tagaagaaat cagagaaatc
     1801 gcgcctactg gtgaccaaag aatggcaaaa aatcctatca ccaatggtgg aatcgatcca
     1861 agaccgctta ttatgccaga ttggaaaaaa tatactttgc aatttgaaaa accaggctcc
     1921 gttaaagcag aagatatgac cgaacttggt aaattcgttc gcgagatcat tgagaaaaat
     1981 ccagaaaatt tccgtatatt tggaccagat gaaacaaaat caaaccgatt gaatcaagtt
     2041 ttcaaaacga caaatcgcca atggatggaa aaaatcgaac cagaaaatga tgaatggctt
     2101 tcgccatctg gtcgcgtcat cgacagtcaa ttatcggaac accaagatga agggttctta
     2161 gaagggtacg ttctgactgg acggcatggt tttttgcaag ttacgaatca ttcttgcgag
     2221 tggttgactc tatgttgact cagcacttca aatggatgcg gaaatcacat gacctttctt
     2281 ggcgaaatga ttatccttcg ctaaatctca ttgcaagctc aacagtcttc cagcaagatc
     2341 acaatggata tagccaccaa gatccaggaa tattgacaca tttggcagaa aagaaggcgg
     2401 agttcatccg tgaatatctg cctgcggatg caaatacatt gctcgcagtc atggataaag
     2461 cattccgctc gtcagaaaaa atcaatttga tcatctcttc caaacatccg cgtgctcaat
     2521 tttactcagc agaagaagca gccgtcttag tgaatgaagg cttgaaaatc attgattggg
     2581 cttcgacagc aaaagaggag gaacctgaat tggtcattgc tgctgcagga acggaatcta
     2641 acttagaagc attagcagct gtaactttgc tgctggaaga attcccaaaa ctaaaaattc
     2701 ggttcatcaa tgtcgtagat ttattgaaat tacgtcatcc aagtcaagat ccgcgtgggt
     2761 tgagcgatga agaatttgat caatatttta caaaagacaa accgatctta tttgctttcc
     2821 acggttatga aacattggtc cgcacgattt tctttgatcg tcataaccat catttaatga
     2881 ttcatggcta caaagaaaat ggagatatca cgacaccatt tgacatgcgt gtagtaaatg
     2941 aacttgatcg ttatcatttg gcaaaagacg cagctttaaa aatcaaagga agtcaagcag
     3001 aagattttgc gaaaaaaatg gatcagaaat tacaagaaca tcaaaactat attcgtgaaa
     3061 atggtattga tctgccagaa gtattggatt ggaaatggaa aaatcttgac caataaaatg
     3121 ataaaatcga tcccgcaaaa tagaattttt tacattgatt aagaaactga aaaaataaat
     3181 aaaaaaaacg gaaaaaagac tggaagaatg gccagtcttt tttttcgttt ttgggaagtg
     3241 aaaaccaaac tatttacctg taagccaccc ataaatccaa tcaaatggtg caaagaaaaa
     3301 atcttccatt ctattccctc ctttaaaaaa gcataacatg atttctattt acgtgagcat
     3361 gaattttgtt acgtttttat tgccgaaaaa taaaaaccgt tcattatgat gtcaaattcg
     3421 tcagtaattg atgcaatgtg ttataataat tcagttaaat caaaaatgaa atggagaaaa
     3481 gacatggacg ggttattacc tttatggaaa gaacgcggaa tgacaagtca tgattgcgta
     3541 ttcaaattaa gaaaaatctt acatacgaaa aaaatcggac atgggggtac gcttgaccca
     3601 gacgtagacg gtgtgttgcc catttgtatt ggaaaagcca caaaagttat cgaatatttg
     3661 acagattctg ggaaaatcta tgtaggcgag ataactctag gtttcagtac aacgacggaa
     3721 gatcgatccg gagaacgggt agaaacaaaa ccagtagata tgtctttatc tgaaaaaaaa
     3781 atagatgaag tgatggcttc atttattggt gagatcaccc agataccacc aatgtactca
     3841 gcggtaaaag tcaacggtaa gcggttatat gaatatgccc gcaataatga aacagtggag
     3901 cgtcctgtta gaaaggctca gatttaccgt tttatccgga caagtgaatt gacaagagac
     3961 gaagtgacag gaaccatttc ttggcgcttc gaagtagaat gcgggaaagg aacctatgtc
     4021 cgcacattag ctgtagatac aggcaagaaa cttggttacc ctgcgcacat gtctgatctg
     4081 acccgaacag caagtgccgg tatgtcccag aaggatgctc tgacattaga agaagtagct
     4141 caagctgtga aaaatgggac aatcgatgat tgtttgctgc caatagaaaa aggagtggaa
     4201 cggttcaaac gagtagatat tactgatgaa gtatggcaga aagtaaaaaa cggtatgaga
     4261 cttgattata ctgtatttgc gctcccagaa atgccgtcag aagaaattgc tttgttttat
     4321 aaaggaaaag ttgtcagtat ttatcagcca aacccgaaag aaaaaaacaa attaaaacct
     4381 agtaaagtat tgagaaatga ggaataacga atgaatatca tcaagatcag acatccttac
     4441 caatcagaac aaattccaga agacgatgtc gttttagtgc tgggattttt cgacggcgtg
     4501 catatcggac accagaaagt cataaagaca ggtatggaaa ttgcacaaaa agaaggattg
     4561 aaattagcat tgatgacttt caatcagcat ccatctattg tctttaaaaa gatcgatcct
     4621 agcagcgtga aatatttgac tagccttaag caaaaagaag aaaaaatggc cgctttgggt
     4681 atagattatt tatatgaagt tgattttact tcttcttttg ctcatttagc accgcaggaa
     4741 tttgttgatc aatacatcgt agggttacat gcaaaatatg ctgtttccgg ttttgattat
     4801 acgtatggac caaaagacat tgcagatgtc gctcattttc ctgaatatgc caaaggaaga
     4861 ttcaaaatcg tcacagtttc taaagaagaa gaacaaggtg aaaaaatcag ctctacccgt
     4921 attcgtggat tactagacag cggacaagta aaagaagcag caaaactatt aggttgcaca
     4981 tatgaggtag aaggggtagt tgtccatggc gatgcacgag gcagactact aggattccct
     5041 acagcaaatg tcaaagtaaa gagcacggtt catttgccta aagaaggtgt ttatgtgtcg
     5101 gaaataaaaa ttggggaaac atggtatcca gccatgggtt caattggaca taatgataca
     5161 ttcggtgaag gacgggagct gactgtagag ttgtatattt tggactttaa tcaagatatc
     5221 tatggtgaac atgtcgcggt acgctggaat caatttttaa gagatcaagt gaaattttct
     5281 agtgtagagg aattgattga acaactcaag gaggatgaac aagcaacagc tgaatatttt
     5341 tctcagcaat aaaacattat gcataaaatc tcttgtttat gtataactag caattacaat
     5401 aaaatgggtg ataaataagc ccaggaaagg ggctgtgaca aaagtcttgt cacagccttt
     5461 ttttccaaat aaatggtttt tcagaagtag cttcttcgga aataagccga aatttcttga
     5521 agctgaccac ttctgtcaca acctctttct ttatagaaat aaaaggacac tagcaattca
     5581 tgaaaacgat tgctagagcg tatcatttgc tttagactat atatcttggt gtacactcac
     5641 tttaaggcgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa aggagtgggt
     5701 actatgtaca aggaattaga aaacaaagtc gcagtagtaa ctggtggctc aaaggggatt
     5761 ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt caactataac
     5821 tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg cggaaaagca
     5881 gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact tgatacggct
     5941 ttagaaaatt ttgggacact ggacgtttgg gttaataatg ctgggatgga aaatcagcat
     6001 ccaacccatg aacttagcct tgaagattgg gaaagagtgt taaaagtcaa tttgactggc
     6061 gtttttatcg gaacgaaagc ggctttaaat tactttacag agaataataa aaaaggaaat
     6121 atcatcaata tgtcttctgt tcatgaacag atcccatggc caacgtttgc tcattacgct
     6181 gcttcaaaag gcggggtaaa attatttacc gaaacggtag caatggagta tgcaccgaag
     6241 ggaatcagaa tcaatagtat aggccctggt gctatccgta cacctattaa tgcggcaaaa
     6301 tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg acgcgtaggt
     6361 gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc cagttatgtt
     6421 acaggtatta cactatttgc agatggtggg atgactttat atccttcttt ccaaggtgga
     6481 aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc agttggctgg
     6541 gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga gatattagga
     6601 actggtattg gagcggtgct tgtcggctta ctcgttcaat tattcgcttc tccagctggt
     6661 atttcagtga ctacattttt gattagttta ctttctggtg cattttgggt tattggacaa
     6721 acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc aatttcgact
     6781 gcattacagt tagtcggaac ctcgttgatt ggtgtcttcg cttttggaga atggtctgga
     6841 agtatgggga aaatcattgg agcagcagca atcattcttc ttgtatttgg ttctgcttta
     6901 acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat tagtctatta
     6961 gcatcaacaa gtattggtta ttgggtatac agtgcattgc ctaaactagt tgatgcaagc
     7021 ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt gatttatgtg
     7081 attttccgta aaccaaccgc attttctgaa gacaagagtt ggaaagcaac gatcgttgga
     7141 gccatcttta gtgtgtcagc attggcttat attttttcag caaatgaaaa tggggttgct
     7201 actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg tatgtacatc
     7261 ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt attgctgatc
     7321 gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa tttcatttat
     7381 taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc gctagaattt
     7441 ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaaggg aaatatctgt
     7501 ctggatcaat aatttttcat tgatccattg actaaagttt acgacataat tgaagttacc
     7561 accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa gacggtcttt
     7621 tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggaatt gattgattgc
     7681 tgtaaaatca cgatattgaa ggttgaaacg catcgtgatt ggtgcattta gcatgaatcg
     7741 ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa aaagattacg
     7801 ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt catgaagata
     7861 atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta ctgagtaaaa
     7921 atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga tatttgctta
     7981 atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca agtcagaaaa
     8041 ttctaacttt tatttctcta aagaaattcg ttctctccta aagtgatatt atatttaaat
     8101 atacctgaaa ataatgggca gtattatgaa agatcactga tattttctcg tattttttcc
     8161 ttattcaaaa gttttgcagg aaaattgctg aaaaaataat tttactaagt agaaaattca
     8221 aagccattca atcagtgatt tttttcagca caagaaagac tccttataaa aatagcagat
     8281 actttgtcaa ttaagtgttt aaccagggat gataaaaagg ataggattga acaaaacctg
     8341 tataaggtga tgaatagttt cctcgccagt aaaaggttta ttcgtataaa gctttatcgc
     8401 caaaatttat catgaccttt tttatcttca atattaaatg aaatcaattg ttcaatcaat
     8461 tcccagttga ttacactttt ccaaggaact ttgatgacgt tatccgtata tacgtagcct
     8521 gcttcttgga tatttttttc aagggcacga atcgctgctt tttccggtgc gatagaaaaa
     8581 tgatttttag caaaactaaa tgcaataata aaggtaccat ggtcagtaaa cataggttgg
     8641 ttccatttta cagttgtgtc tagctgggga tacgtttctg ttatcttagc aaaaaggtct
     8701 ttgatctttt gttgatgttc tttttctttg atttgattga tgatcggctg aaatttttcc
     8761 attctaaaac gcctccatta agtggtttga tttattttac cagaaaaaca atagttatgt
     8821 ataaaaaacg aacaaaacac aagtaatgtt gtaaccgcta tatttatatg ataagatgtc
     8881 tcaaataata aaatagaaaa gagacataaa aatgattgac ttggcaaaga aattcttaca
     8941 cgaaaatata tattttgagt tgtatcatca tcgagccatt tatacaaatg aagatgcttt
     9001 ggtaataaag aaagaacaag gatttacagg tacagagacc aaaagcttat tcttaaaaga
     9061 taaaaaagat aataactacg tatatctgac ttttactacg aaacaaacgg atttcaaagc
     9121 attagggaag atcgtcggta aaaggctttc tattgtttca tcagaacgca tggaagaaca
     9181 aacgggacaa aagcagggcg ctgtttctcc gtttggttat gaaaaacaga ttccgattat
     9241 tattgatgaa gagctcttat caaatgaaaa acttgttttt gcaccaggta gaccagatca
     9301 aacgatggtc atccaaacgg ttgacttaaa caaaatcatc cagctacttg agatgccgat
     9361 atttttgatg ccggcaaacg aaaaaaacaa ttaaccaagt aaaatagtgc atacttggag
     9421 gtgaagcaac atgataagtt atccattatt agaaagaaat gcaacgattg gggttactgc
     9481 accatcctca ggcgtatcag aggaacacca ttcgttactc gaatcagctt taaagagaat
     9541 ggaagaaaaa tctttccaag tagttactgg aaagacgctt tggacgcaag ataaggaaaa
     9601 atcaggtcca gcaagaaaac gagcagatga attcaatcaa atgatgcaag atgaaaatat
     9661 ccaaatgatc tttccgactt ggggaggaga attattgatt gagattctcg attttgtcga
     9721 ttttgatgct ctacaaccaa aatggatagt aggttattca gatattagta cgttgctact
     9781 tgctgtcaca ttaaaaacag ggatagccac tgcacatgga actaacctta ttgatttaag
     9841 aggagagtac ttggatgaaa cgacagcaat gtgggaaagt gtactgaaaa caagagaagg
     9901 agaatcgatt ctacagcaat cttctgaaaa gtatcaaaaa gtgtggcccc atgagcattc
     9961 atcatcttct gttttccatc tcactgaacc aacaaattgg caatcagtca gcggagatgc
    10021 agtaaggatg aagggacgat tattaggtgg ttgtattgat acgatcaggc atttaatcgg
    10081 tactccttat ggagatgtcc aatcttttaa aagaaagtat aatgatggaa agccgatctt
    10141 gtggtttcta gaaaactgtg agctatcgtc agttgattta agaagatcac ttgttcagat
    10201 gaaactggca ggctggtttg atggcagctc ggggatcatg ttcggtagaa gtgcggcaga
    10261 ggatttgtta gattataaaa tgaaggatgt atacgaagat ctgtcaaagg aattaaaact
    10321 accgatcttg tatgatattg attgtggtca tgtaccgcca caactgacat tgattaatgg
    10381 tgcctatgca gaagtagcgg tagaagatag aaaagcgact gttttgcaaa cgtttattta
    10441 aaaaattttt tcctgccttg gagtctatca aagaccaata aatctaaata gaaaagaaag
    10501 tgaaagaagc tgacaaaatg ctgtcagctt ctttttgtat aacatattag aaagtctaat
    10561 tatctataac tgtcacatcg attaactgtt tcacgtcatt gggatttcct gtaggaatag
    10621 ctactacaga tgcattatca atgatttctt gagttgtttc tctaatgaac gctggataag
    10681 atgtttgatc tgcaggcatt tgccaatgtg aaggaactgg ataactagtg attgtcccgt
    10741 catggttact tgaatacact aaaactccct ggtaactgta tttcccagtg agcataacca
    10801 catctttcgg atagactgca ctccctgcat cataaggggc gataggtgtt cctgcaggat
    10861 aatgaataac atgtaattcg aaatcacccg atgtatctct aaaagtatta cctctaatag
    10921 ctagccaaac acgtgcgtac tcaatttgta gatcagaata tcctgctaac ggatctattg
    10981 actgagaaga ctgtgaggag gttgtgtcta ttgttgaaga agaaatattt gtctcaactg
    11041 tactactaga tgtagaagaa gtattgggct ctgagacttt actgcttgtt gaagatgtat
    11101 tcgtttcagt agaagctgat ttctgagtac ttgatttttc agctgaactg gtttgtctga
    11161 ttgaactttt gtctgtggtc gcagatggat cactattttc tttactagga gacgtacatc
    11221 ctacaaggaa taaagagacc aacaataaaa taaaacctaa ctttttcata taaaatacct
    11281 cattattttt ttaataccag tatagcataa tcctgaattt ttgaatccgt aaaaccttgt
    11341 ctatgaatat aaatagagtg tatcttcttt gataaatgtc agaaaaagtg tgacaaataa
    11401 tcatcagtaa aattacttgt cacactattg ataaggagta tttaattgaa gagaatgatt
    11461 aggagaaagg atctccgtac tttttatagt aatcatagaa tccaagtttg tatagacgaa
    11521 tcgttttttc tcttatttct tctgtttcaa aatggactgc ttgaagaatc aagtctgtga
    11581 attctatagg agctgttcca ttcgcagtaa tcaaattacg atctttcaca gcttgttgtt
    11641 ctataaaatc ttctttattc tgataatttt ccgattctaa ccaaggaaat tgcgcatttc
    11701 cggtgtgttt gtaaccttgc aataatccgc tctgagctaa ataatcaact gccccacaaa
    11761 tagcagctac tggtacgtct gtttttaaca atttatcgat tatttgatat aaatgatgat
    11821 tttttatgtc ccatgaattg ccaccgatca taataaaaag gtcaactttt tcaggtattt
    11881 gttcgattaa gtaatcgatt tttgttttta atcctccaat ggaagtgacc tctgattgga
    11941 tagaagcagt tttgttttgc catttctcat tttggtttag tgtgctagta atataagcac
    12001 cttcccaatc ggcatattcc tctaatagaa acgtaatcgc agttttcatt ttcaagctct
    12061 cctttactat ctataacaag gacaacagtt ttaacctaca gacattcatt aaaattgggt
    12121 agcgacagcg aaaacaacaa ttcttaagtt ttagacagag tatacagata gtcaacttaa
    12181 ttaaatacga actaaataaa aatgacaggt aatcattttt gtttaccagc attataacat
    12241 tttgcataca tggatgacaa aagaaccaat tcccacttgc ttgagtacaa tattaaaaga
    12301 aactggaagg caaaaagtaa ttatagaaac gaaaaataaa tcatatcggg cactcagaca
    12361 tgaaaacaac cctagagata tataactagg taaccagttt aatgaaagaa aaaatcatcc
    12421 aagaagtcga ttcttggatt ttttagtgga tttgttcgcc aaatacgttt agtatgcgta
    12481 tatgagaggt tgtaagcaaa gcgttgtggt ctgagcactt aaggaggaat ctgtgaaaat
    12541 agcttttcat atttttgcag attttgactt aatgccgaag gtcagctttg cgattaccgt
    12601 ttatcgagag gctgtaaaag caccgtgtag ctccgatgac aataagcaag catttttaga
    12661 gctaggtttc cttgattcta aatatgcggc ttatttcaat gagttggtgc ttgaacgcga
    12721 ttttaagaag gtcgaaaaca ggcacgactt ttcgacgtaa ggagaaagaa tttcaatgat
    12781 agaaacaata aatcccgcag aaaacaaggt ttctgcgtac attcatatcc cattttgcga
    12841 gcatatctgc tattactgtg attttaataa agttttttta gaagggcagc cagtagatga
    12901 gtatgtggaa atgctgctga atgaaatgaa aataatgcta gaaagtcatc cgacagcgaa
    12961 tttagaaaca ctttacgtag ggggcgggac accaacttct ttatctgcta aacaacttga
    13021 tcgactgtta tcaggtgcta gagagatcct gccttttcaa aatggtaatg agtttactgt
    13081 agaagcgaat ccaggtgatt tgacccgtga aaaacttcaa gtaatgaaaa actatggcgt
    13141 caatcgttta tccatgggag tgcagacatt tgataatcgc ttattgaaaa aaattggacg
    13201 taaacacacc gcagaagatg tgtatcaaac tatgcaattt ttagaagcag aaaattttac
    13261 caatgtaagc atcgatttga tttatgcatt accgggacaa acactagaag gatatctgga
    13321 tactttggaa aaagctttgg ctttggatct gcctcactat tctttgtact cattgatcct
    13381 cgaaaacaaa acaatgttca tgaactgggt acgacaagga agactcgaac tgcctgatca
    13441 ggaaacggaa accagaatgt ttgaagagac gattgaagca atggaaaagc atggacgtca
    13501 ccaatatgaa ataagtaact ttgggctctc cggacatgaa tctcaacata acctcatgta
    13561 ctggaacaat gaccactatt ttgggtttgg agctggtgct agcggttatc ttggagatat
    13621 tcgttaccgc aacaaaggac caatccaaca ctatttacgc ccattacgag caaatgaacg
    13681 cccggtattg gaagaagaaa aattgacacg aaaaaatcaa atagaagaag aaatgttctt
    13741 aggactaaga aaaaaaacag ggattactaa aaaacatttc tatgatcgct atcaaaaaac
    13801 aattgaatcg gtgtacggtg atgtgctaag ggaattagag caagaagggt tacttgttaa
    13861 tgaagaagac cgtgtttatt tgacaccaag agggaccttt ttggggaaca atgtgtttga
    13921 gcgctttttg tttgaaaaat agtttaaaga atatttgttt tttatctgaa tttaaataaa
    13981 aaaagttcaa aaatacaata aaggactatt gaaacgttga tataactgga tttacatcaa
    14041 ttatttagaa ttagcactct tgtgtttaga gtgctaaaaa atggagtctt tctcttgaca
    14101 ggagaagggc ttcttgctat atttataagt ggattagcac ttagaatata tgagtgctaa
    14161 tgagaggtga ggaagatgtt aacaaagcga caaaacgaca ttttacgtct gatcatccaa
    14221 cactatacga gtagtggcgt gcccgttggt tccaaaacat tgatgtctga gggagtcaag
    14281 gccagctcag caacgattcg caatgatatg aaagcattag aagatgaagg attgctgttg
    14341 aagactcact cttcttctgg gcggattcca tctgctttag gctatcgtta ttacgtggat
    14401 catttgttaa aacctgctcg cgtagcagaa gatgatttgc tgctgattcg tcagtctcta
    14461 ggcagagaat ttcatgaaat caatgagatt attaagcaat ctgctgagat attatctgag
    14521 ctgacaagct atacgacttt ttcattggga cctgaaataa aagaccgccg actgactggc
    14581 tttcgtatcg tacccttgaa taatcgacag attattgcta tcgtcgtaac agatatgggg
    14641 aatgtagaaa gccaagtgtt cactcttccc gaatatatgg gaagtcagga actagaaaaa
    14701 atggtacaga ttgtaaacga ccgtctagtc ggagatcctc tgatgacggt gtaccataaa
    14761 cttcgtacgg agattccgat gattcttcat aaatatttcc aaacgacaga agggatgtct
    14821 cagttatttg acaccgttct tggtcatgtt tttgaagata aggtttatgt cagtggacaa
    14881 atgaatctct tggattatga gccccgtcaa gatcttgatc agttcaaatc gatgttttct
    14941 tttatgaaag actcagacgg acttacacaa atgatcactc cgatggatag caatattcat
    15001 atcaagattg gtgctgaatt aggcaatgat ttattgcaga atatgagtat gattcaagca
    15061 agttacgaga tagatggtca tggaagaggt acaatagcct tgttagggcc tactagtatg
    15121 ccttattctc ggatgttcgg tctagtcgat gtttatcgaa gagaactagc agctaaactt
    15181 gctgattact accgttcgct tgacttgtca aactcgtaat gtgaataacg aagctatttg
    15241 cagctgatcg ttctctcttt cttagtaaga gcaatcaaac agctgactga ataatgaaag
    15301 gaagactgta tcgtgaaaga aaatcaagaa gaactagata aagaaatgac agatgctcag
    15361 ccagaaccgg aaatcgatgt cgaagctgca gaagattcgg gtatttcaga agctgaagcg
    15421 gaagaattcg aaacagcgaa attaaaagct gagttagaag aaatggaaga taaatacttg
    15481 cgggctcgtg ctgaaatcgc aaatatggca aatcgaggaa aaaatgagcg tgagcaatta
    15541 caaaaatacc gttcacaaga tttagccaaa aaattacttc catcaattga caacctggaa
    15601 cgtgcattag ccacagaagt gtcagatgat caaggggcag gtttgaaaaa aggcgtggaa
    15661 atggtcttgg aaagtttgcg taacgcactg gaagaagaag gaattgaaaa aatccctgct
    15721 aaaggagaag cctttgatcc taatcttcat caagcagtac aaactgttcc agcgactgaa
    15781 gatacaccag ccgatacgat tgtagaagta ctacaagaag gctacaaatt acatgatcgc
    15841 gtgttaagac caaccatggt cattgttgca caataattgt gcggatataa aacttacact
    15901 ttcaattaaa gaaggagacg ttaattatga gtaaaattat cggtattgac ttaggtacga
    15961 ctaactctgc agtagcagta ttggaaggaa acgaagcaaa aatcatcgct aacccagaag
    16021 gaaaccgtac aacaccttct gttgtatcat tcaaaaatgg agaaatccaa gtaggggaag
    16081 ttgcaaaacg tcaagctgtg acaaacccaa atacgatttc atcaatcaaa cgccatatcg
    16141 gtgaagcagg ttacaaagta gatgtagaag gtaaatcata tacaccacaa gaaatctcag
    16201 caatgatcct tcaatatatc aaaggattcg cagaagacta tttaggtgaa aaagtcgaaa
    16261 aagcagttat cactgtgcca gcttacttta atgatgcaca acgtcaagca acaaaagatg
    16321 ctggtaagat cgctggtcta gaagtagaac gtatcgtcaa tgaaccaact gcagctgctc
    16381 ttgcctatgg tttagataaa acagatcgcg acgaaaaaat cttagtattc gaccttggtg
    16441 gcggtacttt tgacgtatct atccttgaac taggagacgg tgtctttgat gtattgtcaa
    16501 cagctggcga caaccatcta ggtggggacg actttgataa caaaatcatc gactacatgg
    16561 tagcagaatt caaaaaagaa aacagcattg acttgtctca agacaaaatg gctttacaac
    16621 gcttgaaaga cgctgctgaa aaagcgaaaa aagatttatc tggcgtatca agcacacaaa
    16681 tcagcttgcc atttatcaca gcaggagaat ctggaccatt gcatttggaa atgacattga
    16741 cacgtgcaaa atttgatgaa ttaacagctg acttagtaga acgtacaaaa gtaccagtac
    16801 gtcaagcgct aaaagatgca ggtttatcac aatcagaaat cgacgaagtt attttagtcg
    16861 gtggatctac tcgtatccct gcagttgtag aagcagtacg taaagaaaca ggaaaagaac
    16921 caaacaaatc agttaaccct gatgaagtag ttgctatggg tgctgctatc caaggtggcg
    16981 tgatcactgg tgatgtgaaa gatgttgttt tacttgacgt tacaccattg tcattaggta
    17041 tcgaaacaat gggtggagtg ttcactaaat tgattgaccg caatacaacg attccaacaa
    17101 gtaaatcaca agtattctca actgcggcag ataatcaacc agcagtagac atccatgtat
    17161 tacaaggtga acgcccaatg gctgctgaca acaaaactct aggaagattc caattgacag
    17221 atattccagc agctcctcgt ggtattccac aaatcgaagt aacattcgat attgataaaa
    17281 acgggattgt aaatgtatct gcgaaagatc taggtacaca aaaagaacaa aaaatcacga
    17341 tcaaatcttc ttcaggtcta acagacgaag aaatcgaacg tatggtaaaa gatgcagaag
    17401 caaatgcaga agcagataaa gctcgtaaag aggaagttga cctacgtaat gatgtagatg
    17461 cattgctatt ctctgtggac aaaacattga aagaattaga aggcaaagtg gacgaagaag
    17521 aagtgaaaaa agcagaaaca gctcgcgatg aattgaaagc cgctgttgaa gcaaacaaca
    17581 tcgaagaaat gaaaactaaa cgcgatgcat tgaacgaaat cgttcaaaac ttgactgtta
    17641 aactatacga acaagcggca caacaacaag ctcaagaaaa cccagaagca gctcaaggcg
    17701 gagcagatga tgtcgttgat gctgattttg aagaagtcga tggcgacgat aaataatcag
    17761 ttcactgaat ttcgagagaa ggccaaagtt gctactttgg ctttttcttt tgaaaaatag
    17821 tttgaagtat cggtttttgt ttcaaacaaa atatgatatg ataacaagcg acgtgaaaat
    17881 gatgagaacc aataggagga aagcctatgg caacaaaacg tgattattat gaagtcttag
    17941 ggctctcaaa aggagcatca gaagatgaaa taaagaaagc ttatcgtaag ctttcgaaaa
    18001 aataccatcc tgacatcaat aaagaacctg atgcggaaga aaaattcaaa gaagtatcag
    18061 aagcatatga gattttaagt gacccgcaaa aacgcgctgc atatgaccaa tacgggcatg
    18121 caggcacaga tccaaattac ggtgctggtg gagctggtgg cggatttggt ggattcggcg
    18181 gcttctctgg tggcggcttc ggtggttttg aagatatctt cgactctttc tttggaggag
    18241 gcggtcggtc agtcgatcca aatgcgcctc gtcaaggtgc tgatttgcaa tatacggtcg
    18301 acttgtcttt tgaagaagcg atttttggtg tagaaaaaga aatcaagtat aatcgggaag
    18361 aaatctgtca tacttgtggt gggaacggtg caaaaccagg cacacaacca actacttgtc
    18421 ataaatgtca tggtgcaggt acgattaatg tagaacgcca aacaccactt ggacgagtga
    18481 tgagccgtca aacttgtgat gtttgtcatg ggacagggaa agaaatcaaa gaaccttgtc
    18541 caacctgtca tggctcagga catgagaaga aagcacattc agttaaagtg aatgtaccag
    18601 ctggtgtaga agaaggacaa caaatgcgtt tagctggtca aggagaagct ggagaaaacg
    18661 gcggaccatt tggtgatttg tatgtggtct tccgtgtaga agaaagtgat atctttgatc
    18721 gtgaaggttc tgaaatttac tatgaattgc ctttaagttt tgtacaagca gctttggggg
    18781 atgaagtcca agtaccaact gtccatggag atgtgaaatt gaagatccct gctggaacac
    18841 agacaggtac gaacttccgt ttacgtggaa aaggcgctcc gaagttacgt ggtggtgcaa
    18901 caggcgacca acatgtcaaa gtaaaactga tcacaccgaa aaatctaaat gaacaacaaa
    18961 gagaagcatt acgtgcattc gctgaagctg gcggcatcaa agttgaagaa cagcaagaag
    19021 acggtttctt cgataagttt aaagatgctt ttggcgggaa aaagcggaag tagtttttct
    19081 agtaaaaacg ctaagtcatt aataattaaa aaccagacga acaatcatat gttcgcctgg
    19141 tttttttgta gttgtcatgt ttgtaccaac aaaaaagcca gctttagaat atgataatcg
    19201 tcatagtttc taggggtata accagtgaga gaatggaact tgttcaatcg atattgcaag
    19261 gtgtttttat gaataaataa ttcttctgag caatttttga tgcttccgtt atttttactg
    19321 aagcatgcca aagtcttctt gaacaaatgt acttcttctt ctgtcaggct tttcaaaata
    19381 cgttttacat ataatgttcg ttgatctgac gggattgctg gtaaaacgat cccgaggtct
    19441 aaatcttcaa aaaagcggat ctcgttcaca gattgatgga aagctatcca ttcgcaaact
    19501 tgtttggcat gaaggaaaga ttgcggtgtc tcttgctctg tataacaaag cgacccaatt
    19561 ccaaagttta gctgtaactg atacatttta ttaaactttg tctggatttt atcaagcaat
    19621 gatagcaatg aggctctgga aatccgattg actaggataa tgatttgttg tctatgtaca
    19681 gtgagcaaag cctgtccgta ataagagaag accctttcga gcaactccaa taaaggattg
    19741 atccgttctt gacacaattg ctgattgtca gaaagttgtc ccacaactac ataatatgga
    19801 gaagaaaaat caagctcaat taagtgaggg tggtatcgac tatgtacttg atatcatcaa
    19861 tgaaccgatg gtactttctt atgcgatgga acaggtagag cgattgcttg tttgtgcagg
    19921 aaaacaagcg atgcggctga tacgattagg tgagaaaata agaagataaa aatacacggt
    19981 cccttcatgg cgaccgtgca tttttggttt ttattaaata tttaatactt tattcaagaa
    20041 atcttttgtc cgttcatttt gcggattttc aaagatttgt tgaggcgttc catcttctaa
    20101 aaagtttccg tcgtcaatga acatgacgcg attggccact tctttagcga accccatttc
    20161 gtgcgtaacg ataaccatcg tcataccttg ttctgctaac tttttcatga cgttcaagac
    20221 atcaccgacc atttcaggat cgagtgcaga agtaggctca tcaaaaagca tgatgtcagg
    20281 gttcatcgct agagcacgag caatagccac acgctgtttt tgtccaccag aaagcatttc
    20341 aggatacata tcttttttat cttctagacc aactgtctgc agtaatttga ttgctttttt
    20401 ctctgcttct tctctagatt ctttcttcag gtccatcggt gctaaaatga tgttttccaa
    20461 aatagaaaga tgagggaata ggttaaagtg ttggaatacc attccgatat gttgtctcac
    20521 caagttgata tctgtgcttt tatccatcaa gtgggcgccg tcaataataa tttcaccgtc
    20581 agaaggttct tctaaacgat tcaagcaacg caagaatgta ctttttcctg aacctgaagg
    20641 gccaatgata cagacgacat ctccttcgtt gatggataca ttgatgtcgt ttaatactgt
    20701 gttatcgccg tattttttga ctaaatgctc aactaagatt ttttctgcca tcttacttca
    20761 ccttcttttc taaaactttt gctaattttg tcaatgctgt gatcaaaatc aagtacatga
    20821 tcgcaatgat caagtaaaca tacgtacttt gtgttgtacg tgcaacaatg atcttaccag
    20881 tttgcagcaa ttcgactaca ccgattgcgg aaatgatcgt tgtgtctttt aatgagataa
    20941 cgaactgatt gacgaatgat gggatcatga tgcgtatcgc ttgcggcaaa atgatttttt
    21001 gcattgtccg attataagaa agacctaaac ttctggaagc ttccatttgt ccgacaggaa
    21061 cggcgttgat cccgcctcga acaatctctg cgatataagc acttgcgtta agagtcaagg
    21121 tgataacacc agcaaggaag tcaggaatcg taaatccaac gataccaggc aacccaaaga
    21181 aaataaagaa cgcaagaacc atcattggaa ttcctcggat gatatcgaca taaatactag
    21241 caattgtgcg aagagcgcgt attggtgcta cactgaataa tccaaaaatc acaccgatta
    21301 ctatagccaa agcaaaggag atcaaagtta ggagaattgt tctccaaagt cctttcaaca
    21361 atgcttgata gttgttttgg ataagtccaa gaaatgttga ttcatcagca gtggaagact
    21421 cactggaagt ttctgaacca tccgctacat attctttaat aatcttatcg tattcgcctg
    21481 ttctttttaa ttctttcaat ccttcattga acatattaag aagctcagga ttttgtcctt
    21541 ttttaacggc aaatgcatat tttccgccag attctttttt gatcggtgtt tccaattctt
    21601 gtccttgttt gatcgcataa ccgattaccg gataatcatc catcattgca tcgattgcgc
    21661 caacttttaa cccgtcgtac aattgatcag cagcatcaaa atatttgata gaatagccat
    21721 attcagattc atggtcgctt aaaaattcag cactttcggt accaactttt gctccgactg
    21781 tttttccttt caaatcttca taagacttga tcgaagtatc tccttttttg acagctaatt
    21841 ggatcccgct atcaaagtaa gaatcagaaa aatcatagct tttctttcgt tcatctgtta
    21901 cagtcattcc ggcaatcatt ccgtctgctt gtcctgattc caaagcttgc aaagcagcag
    21961 agaaaccacg gtagtccatt cggatgttaa atccttgcag tttggcgatg ttttccatca
    22021 attttacatc gatgccttcg tactcgccct ttgcgttttg aaattcaaat ggagcaaaag
    22081 cagagtcact ggcaattacg tatacgtctt ttttgggctc tattttttgc attgtctcgc
    22141 ttgtgcttga ctctgccgtt gtagtgttgt cagcttgtgc aacatgacca aacgcaagtg
    22201 tcacaagtcc tgtcattaag aaaacaaatg agtgaaataa atgctttctt ttcataatca
    22261 acctcctaaa tttccaatat tttattatta taggcgaaaa gtgctgaatc agacaagact
    22321 gtaagctcga aaaaatgaaa aaaatgcgag tttctaacat tttatgttat aaaacataac
    22381 atgtataata agataaaaaa gtggcgaaag tatgttcggt gtggtataat tttttagtaa
    22441 ggaatgcctt tgctttcctt ttctttttat tggtcgtaaa atgaaaagga atgaatgaaa
    22501 aggaggacga agaatgattg agagagatac ctcccgacac tttgagcttg tctcgaaata
    22561 ccaaccggct ggtgatcagc ctgaagcgat taaccaatta gtggatggcg tagtcggcgg
    22621 gaaaaaagca caaatcttat taggtgcgac tggaacaggg aaaacatata cgatttccaa
    22681 tcttattgag aaagtgaata aaccaacact gattattgcc cataacaaaa cattggctgg
    22741 tcagctgtac ggggaattca aggaattttt ccctaacaat gctgttgaat attttgtttc
    22801 ttattatgat tattatcagc ctgaagctta tgtgccttca agcgatacgt atatcgaaaa
    22861 agattcaagt gtaaatgatg agatcgacaa attacgacat tcggctacta gttcattatt
    22921 agaaagaaat gatgtgatcg tcattgcatc cgtttcatgt atttttggat taggctcgcc
    22981 ttttgagtac caaaaacaag tagtttcgat tcgacaggga gcagaactag atcgcaatca
    23041 gttgattcgt gacttagtca gtatccaatt cgaaagaaat gatatagatt ttcagcgcgg
    23101 tcgttttcga gtgcgcggtg atgtcgtgga gatttttccg gcttcaagag atgaacgtgc
    23161 cttacgtgtg gagttttttg gagatgaggt cgaacgtatc cgagaagtca atgcactgac
    23221 aggtgaagtt ttaggagaaa cggaacacgt agcaatcttc ccggcgactc actttgtgac
    23281 gaatgatgaa catatggagc atgccgtagc gaatatcaaa gcggaattag aacagcgttt
    23341 aactgtcttg cgaaatgaaa acaaacttct ggaagctcaa cgtttagaac agcgaaccaa
    23401 ttacgatatt gaaatgatgt tagaaatggg ctacacgtcg ggaattgaaa attattctcg
    23461 gcatatggac ggacgtaaag aaggtgaacc gccttatacg ctattggatt tctttcctga
    23521 agattttttg atcgtggcgg acgaatcgca cgtaaccatg cctcaaatca gaggaatgta
    23581 taatggtgac cgagcgagaa aacagatgct agtagattac ggtttccgtt tgccatctgc
    23641 tttagacaat cggccacttc ggttagaaga gtttgaaaaa catgtcaatc agatcattta
    23701 tgtctctgcg acgccaggac catatgaaca tgaacaaaca gataccgtta tccaacaaat
    23761 catccgtccg acagggctgc ttgacccagt gatcgaagtt cgtccaatca tgggacaaat
    23821 cgatgatcta gtaggcgaaa taaatgaacg agtcgaaaaa gaccagcggg tgtttgtaac
    23881 gacattgacg aaaaaaatgg cagaagacct cacagattat ttcaaagaat tagggatcaa
    23941 agtcaaatat ttgcatagtg atatcaaaac gctggaacga acagaaatca ttcgagatct
    24001 tcgattaggt gaatttgatg tgttagttgg aatcaatttg cttcgtgaag gattagacgt
    24061 acctgaagtt tcccttgttg ctatcttgga tgcggataaa gaaggctttt tacgtagtga
    24121 acgttcctta gtccaaacga ttggacgagc tgctcggaat gaagaaggta aagtaatcat
    24181 gtacgcagac aaagtaacag attctatgcg tctagcgatg gatgaaacgt caagacgtcg
    24241 tacgattcag cagaagtaca atgaagaaca tgggatcgtt ccaaaaacga tcattaaaga
    24301 aatccgagat ttgatttcta ttacgaaaga atcggaagat gatacaaaag aagcggtaca
    24361 agtctcgtac gaagaaatga cgaaggaaga aaaagacacg ttattgatga aattagaaaa
    24421 agaaatgaaa gatgcggcaa aagctttgga ttttgaaaca gccgcgaatg tgcgagatat
    24481 gattttagaa ttaaaagctt caaagtagga ggacaacatg gctaacgata aaattacgat
    24541 tcatggtgca cgtgcccata acttgaaaaa tatagatgta acgattccac gagataaatt
    24601 cgtggtcgtt acaggtttgt ccggttctgg aaaaagttcc ctagcatttg atacgctgta
    24661 cgctgaaggg cagcggagat acgtagagag cctttctgct tatgctcgtc agtttttagg
    24721 acaaatggat aagccggacg tagatagcat cgacggattg agtccggcga tttctattga
    24781 tcagaaaaca acaagtaaaa atcctcggtc gacagtaggt actgtgacgg aaatcaatga
    24841 ctatttacgc ttgttgtttg cacgtgtagg acatccgatt tgtcccaacg atcatatcga
    24901 gattaccagt caatcagtag atcaaatggc agatcaagta atggctttgc ctgagcgcac
    24961 aaagatccaa gtgttggcac cgatcgtggt aaagaaaaaa ggacaacata aaaaaatatt
    25021 cgaacgaatc caaaaagagg gatacgtccg cgttcgagtc gatggtgaga tctatgattt
    25081 aagtgaagtg cctgagctgg agaaaaacaa aaaacatgat atcgcgatcg tcatcgaccg
    25141 gatcattgtg aaagaaggga ttcgttctcg cctatttgat tctttagagg cggccctgcg
    25201 attagcagac ggctatgctc tggttgacgt gatcggtgaa gaagaaatgt tgtttagcga
    25261 acactatgcc tgtccttatt gcggatttac tgttggcgaa ctagaaccaa gactgttctc
    25321 attcaatgcg ccttttggag cttgtccaga ctgtgatggt ttaggtgtca aattagaagt
    25381 cgatcaagat ttagtgattc cagatagaac gaagacactt agtcaaggtg ctatcgttcc
    25441 atggaatcca atcagctctc agtattatcc tcaaatgtta gcgcaggctt gtgaaagttt
    25501 cggtatcgat ttagatacac cattcgaaga attaccggaa gagcatcagg agatcatctt
    25561 gaatgggaca gatgatcttt tccatttcca ttatgaaaat gattttggtg gcgttcgcga
    25621 tgtagaaacg actttcgaag gggtattgaa gaatatcgag cgtcgttatc atgatacgaa
    25681 cagtgatttc acgcgtgagc aaatgcgtct ttatatgaca gaattaactt gtcagacatg
    25741 taaaggttat cgattgaatc cccaagctct atctgttcaa atcaatggaa cgaatatcgg
    25801 gcaaacaaat gagttgtcta ttcaaaaagc agtagatttc ttctcaaact taacgttatc
    25861 tgaacaagag aaagtaattg ccaaaccgat tttaaaagaa gtaaatgaca gactttcatt
    25921 cttggaaaat gtgggactag actatctaac tttgagtcgc gcttccggaa cattatctgg
    25981 gggagaagcg cagcgaatca gattggctac acaaattgga tcgaatcttt ctggagtttt
    26041 gtatatttta gatgaaccat cgatcggatt gcatcaacgc gacaacgatc gactgattgg
    26101 ctcattaaag aaaatgcgtg atcttggcaa caccctcgtt gtagtcgaac atgatgaaga
    26161 tacgatgtgg gcagcagatt atttgattga cgtcggacct ggggccggag aacaaggcgg
    26221 acaaatcgtg gctgcaggca ctcctgaaga agtagctaat gatgaaaatt ctttgacagg
    26281 gaaatatctt tcagggaaaa aatcaatccc tgtaccaaaa gaacgccgta aaggtaatgg
    26341 gaaagcaatc aagatcactg gtgcttcaga aaacaatctg aaaaatatta gtgttgaatt
    26401 tccattagga gaatttgtag cagtcacagg tgtgtctggt tctggaaaga gtacattggt
    26461 caatagtatc ttaaagaaat cgttagcgca aaaattaaat aagaattctg ctaagccagg
    26521 taaattcaag acaatttccg gctacgaaag tatcgaaaag atcatcgata tcgatcaaag
    26581 cccaatcggc cggacgccga gaagtaatcc agcgacttat acaagtgtat ttgatgatat
    26641 ccgtgggtta tttgctcaaa cgaacgaggc aaaaatgcgg ggttataaga aagggcgttt
    26701 tagtttcaac gtaaaaggcg gtcgttgtga agcttgtcgc ggggatggaa ttattaagat
    26761 cgaaatgcac tttttgcctg atgtctatgt tccttgtgaa gtatgtcatg gtaaacgata
    26821 taattctgaa acattagaag tgcattacaa aggaaaaagc attgctgata ttttggaaat
    26881 gacagtagag gatgctgtag aattcttcaa gcacattcca aagattcatc gcaaactgca
    26941 aacgattgtt gatgttggct taggttatgt gacgatgggg caaccagcaa cgacattgtc
    27001 cggtggtgag gcacaacgga tgaaacttgc cagtgaattg cacaaaatct ctaatggaaa
    27061 gaatttctat atactagatg aaccaacgac aggacttcat agcgatgaca tcgcccgctt
    27121 gttgcatgta ttacaaagat tagtagatgc tggtaacaca gttttagtga ttgaacacaa
    27181 tctagatgta atcaaaacag cagattatat cattgattta ggaccagaag gtggagaagg
    27241 tggaggaacg atccttgcga ctggaacacc agaagaaatc attaacgtaa aagaaagtta
    27301 tacaggtcac tatttgaaaa aaataatggt ataaagagga tagatagctg agatagttaa
    27361 aaaaacataa aaaaatgaat ttttgcttgt tttattaata tcagtgttct agaatcatta
    27421 catggtagtt ctttctacac tgattggtat agataaaaac ttcctataag ttcaatccgt
    27481 tcaaaaaaga attgagcaat cggaggactt tcctttgatt gcttttttct ttatggtatg
    27541 atattccttg tgtagttgga aaagaaaaac aagagattat gagtaagtga gagataatga
    27601 tgaacagtat tgaaaatgaa gaatttatcc ctatattatt aggaagcgac atgaacgttt
    27661 atggtatggc acgttctttt aatgaagcat acggcaaaat ctgccaagca tatgcttcgg
    27721 atcagttagc ccctacccgc tacagtaaaa ttgtcaatgt agaagttatt ccaggatttg
    27781 ataaagaccc tgtatttatc gagacgatgc ttcgtttggc aaaagaaaga tattctgata
    27841 agagtaaaaa atatcttttg attgcttgtg gtgatggata tgcagaattg atctcccaac
    27901 ataaacaaga gctgtcagaa tattttatct gtccatatat tgattattct ttatttgaaa
    27961 ggctgatcaa taaagtaagc ttttatgaag tttgtgaaga atatgatctt ccgtatccta
    28021 aaacattgat cgtcagagaa gaaatgttag taaatggaca tttggaacag gaattgcctt
    28081 ttgaattccc agttgctttg aaacctgcaa acagtgtgga gtatctttct gtccaatttg
    28141 aaggaagaaa aaaagctttt attttagaga ctagagaaga gttcgattta attttaggcc
    28201 ggatatacga agccggttat aaaagtgaaa tgattgtaca agattttatc ccgggtgatg
    28261 atagtaatat gcgtgtgcta aatgcgtatg tagatgaaga tcatcaagtt cggatgatgt
    28321 gccttggtca tccactatta gaagatccta caccagcatc gattggtaat tacgttgtga
    28381 tcatgccaga ttataacgaa aaaatctatc aaaccattaa ggcatttttg gaaaaaatcg
    28441 aatatacagg ctttgccaat ttcgatatga agtatgatcc tagagatgga gaatataaat
    28501 tatttgaaat caatttgaga caaggtcgta gcagtttctt tgtgacattg aacggcttga
    28561 atctggctcg ttttgtcaca gaagatcgag tgttcaacaa accctttgtt gaaacgacct
    28621 atggaacgaa tcaatcagat aaagcaagat tatggatggg tgtgcccaaa aaaatctttt
    28681 tagaatatgc tcgggaaaat gaagacaaaa aactagctga gcaaatgatc aaagaaaatc
    28741 gttacggtac tactgtattt tatgaaaaag accgatcaat caagcgctgg ttgttgatga
    28801 aatatatgtt ccataactat attccaagat tcaaaaagta ttttcatgtg aaagaaggct
    28861 agttcatgga gaattttttc agtattttag gcggaatggg cacgatggcg acagaaagtt
    28921 ttgttcgctt gatcaaccac cgaacgaaag ctacaaaaga tcaagaatat ttgaattatg
    28981 tcttattcaa tcatgcaaca gttcctgatc gtacagctta tattttagat cgatcagaag
    29041 aaaatccaat gccgtttctg ctggatgata ttgagaaaca aaatttattg cgaccaaact
    29101 tcattgtgtt gacttgtaac accgcacact acttttttga agagttacaa gcggcaacgg
    29161 atattcctat cttgcatatg ccaagagaag cagcaaatga acttgtccgt cagcatacga
    29221 cagggagagt agcgattcta ggaacagaag gaagtatgaa agctggaatc tatgaacgcg
    29281 aagtcaaaaa tcttggtttc gaaacagtga ttcctgatac tgcgcttcaa gaaaaaatca
    29341 attacctgat ctatcatgag atcaaggaat cggattattt gaatcaagag ctctattacg
    29401 aaatcttaga ggaagcggta gagcgcctaa actgtgaaaa agtcatccta ggttgcacag
    29461 aattatcttt gatgcatgaa tttgctgaag ataatcatta cccagtgatc gatgctcaat
    29521 ctattttagc ggatcgaacg atcgaacgtg cattagctga aagaagcgag gcattggata
    29581 cagcatcgga aaagtaaaaa aattggattt ggggcaaata tcctaaaaga tatttggtcc
    29641 aaatcttttt gtaaaagaaa aagaaaactg gtaatgatac gcccgggatg atataatttt
    29701 attatgcaca atgtgagagg ggttcgtaaa tatgacagat aatttgcaat tagtcatcat
    29761 aacaggtatg agtggtgcag ggaaaacagt agctatccaa agttttgaag atatgggcta
    29821 tttttgtatc gataatatgc cgcctcgatt gattccaaaa ttctgggaat tgatcaaaga
    29881 atcaggtaaa gtcacaaaaa tggctttagg agtggatttg cgttctcgtt ccttctttga
    29941 agaaatccaa aacatgctga tcgaaatcga aaatacgaac ttcatcgaca cttctatctt
    30001 atttttagat gcttcagacg aagaactagt ggctcgctac aaagaaacaa gacgagcaca
    30061 tccaatggcg atggacggtt tgatcactga agggatacgt aaagaacggg cgatactaga
    30121 cgacttgaaa gcccaagctt ccgtgatcat tgatacgaca acattgacac cacgacaatt
    30181 acgtgaaaaa atcaatgagt cattcaaatc aaagggagac caaggatttc ggatcgaatt
    30241 tgtatcattt ggttttaaat acgggttgcc gatcgattcg gatattgtga tggatgtgcg
    30301 ttttttgccc aaccctcatt atatcccgga actaagacca ttgaccggaa aagatcagcc
    30361 agtctatgat tatgtgatgt cctttgatga aacgcaatca ttctaccata aatttttgga
    30421 tcttctggaa actgtattgc ctggatacat taaagaagga aaaagcagct tgacagttgc
    30481 aatcggctgt acaggcggtc aacaccgttc tgtggcattg actgaacgtt tggcaaatgc
    30541 tttagggaaa aactataaag tcaatgtgac acatcgggac aaggataaaa ggaaagagac
    30601 ggtgaaccgg tcgtgaaaat gaaaacctac cggataagaa aaccaaaggt cgtagtggtc
    30661 ggcggtggca ctggtctgcc agttatcctg aaaagtttac gaaatcaagg agcagatatc
    30721 acagcagtag tgactgttgc agatgatggt ggaagcagcg gtgcaatcag agaatcgatc
    30781 gctatggctc ctcctggtga cttgagaaat gtcttagtgg cgttatcgga tatgccacaa
    30841 ttctatgaag atattttcca ataccgtttc aaaaaggaag atcagttttt agccaatcat
    30901 acgatcggta atctgatcat tgccgcagtt tccgagatgc ggggaagcac ttatgaggcg
    30961 attcaattat tagcaaaaat gatgcatgta gatggacatg tttatccttc ttcagaaact
    31021 cctttgacct tgcatgctgt ttttaaagac ggaacgactg cagtaggaga atctaaaatc
    31081 gcgatcaacc gtaaaaccat tgaccgtgtg tatgtctgca acacaaatga taaaagtgaa
    31141 gccaaagcgg ctcgaaaagt cgtttcagca attatggacg ccgacatggt cgttttggga
    31201 cccggaagtt tattcaccag tatccttcca aatttagtga taccagaaat cggcgaagca
    31261 atgctcaaga caactgctga aaccgtctat atctgtaata tcatgaccca aaaaggggaa
    31321 acggaacatt tcacagatgc cgatcatata cgtgttttac acaagcattt gaatcagcct
    31381 tttatcgata cagtattggt caatacggaa aaagtgcctg aagattatat ggacccagag
    31441 atctatgatg agtatctagt acaagtcgca cacgacttca acggattgag aaatgaagga
    31501 tgtcgagtga tttccaccga ctttttagaa ttgaaagatg gtggtgtctt ccacgatgga
    31561 gacaaagtag tagaagaatt gttccgttta gttttcggtt caaaatatta agcaatttat
    31621 tgttggagaa aggaggagaa taggatgtca tttgctgcag atgtgaagaa agaactaact
    31681 ggtttggaag ttcatcgaga acatgcgaaa gcggaattag ctgctttgct gcgaatgaac
    31741 ggctctttag gtattgtgaa ccagcagttt attttgaacg tccaaacaga aaatgctgca
    31801 attgccagac gtatctattc tcttttgaaa gatcattatc aggtacgaag tgagctactc
    31861 gttcgtaaaa aaatgaaatt aaaaaagaac aatgtctata tcgtccggtt gaagcaaggc
    31921 acaaaagatg tgttgatgga tcttgatatc atggatggta tgatgttcaa tggacacgtt
    31981 tcggacgaga ttatgggaaa ttcgcaaaaa atgcgttctt acttacgtgg tgcttttatg
    32041 gcctcaggtt cagtcaataa tccggaaacg agtcgttatc atttggaaat attttctatt
    32101 tatgaacagc ataaccaaga catttgcgat atgttaaatt attacggtct acatgctcga
    32161 acgcttgaac ggcgcaatgg ctatatttcg tatttaaaag gagcagaaaa aattgctgat
    32221 ttcttaacat tgattggtgc aaccaactca atgctccgat ttgaagatgt tcgaatcgta
    32281 cgggatatga gaaattcggt caatcgtcta gtcaattgtg agacagcaaa tctgaacaag
    32341 acgattgatg cggcttctaa acaaatcgaa aacatcgaat tgatcgaagc aaaggtcggt
    32401 ctacatgccc ttcctgaaaa acttcaggaa atcgcagaac ttcggcttca acatccagag
    32461 gtcagcttga aagaattagg ggaaatgatt ccctcaggtg caatatcaaa atcgggaatt
    32521 aatcaccgaa ttcgcaaaat aaatgaattt gcggaaaaac tgcgtgaaga agcagtttaa
    32581 tccaaagaaa aaactgatac atgaagcagg agatgcgaat gtatcagttt tttttaaaag
    32641 ctatacttgc ttttcttccg tcaaactata attccaactt tactggagta acataatgtt
    32701 gaaaggcttc tattgttttt taatacaatc gtataaaaag cggggagaca actgaatgaa
    32761 gattttagtt ttatacataa acttttgctt aatatagaag tcctgatata gataggatca
    32821 agtatttatc tataactgga gattaattaa aaactggaag gaactgctcg atcaaccttg
    32881 agaactgttt ttcttacttc tggtcaagat tctcgtggtt ggtggataag gatggttgat
    32941 aagttcattg gattagctag tcgaaaacaa ccaggtgtaa ttaatgtaga agttttaaca
    33001 aggggatgga gtgatttatt cagtgataat cataagttca taaatagtaa ttgggatgta
    33061 tggttggtat acgttatacc tgaacttgaa ctatcaagat ttggtttaga atttgagaat
    33121 cgatgtttat aaagggaggg caaaaaatgt gcttttttac aacttttact atcattatga
    33181 ttgcagtact ttttattttt ctagatatag caaagcgaaa tacagcattt ttattatatc
    33241 gggttttact tcgagcaggt ctcataacat ttatatcgat cgttggattt tttctattca
    33301 cagttattgt atttatttgg agaactacag caccaccatt gccagagatt acatatggag
    33361 aatttccgtt tagtcttgaa tatgaaatag atgaggaaag aattttgatt gaagatacgg
    33421 tgattgcaga atttatagga agttttagag ggaatgtgac aactcaacct agacgaagtt
    33481 ggagtataaa acttttaaat aatagtggag agttgaaagc atttcattat gaattcttaa
    33541 taatagaaag tgaagatgta acaatcactt taagtagtgg ctctgctgga tattttatga
    33601 atgatggtgg aagcaagcca cagctcacag tccgtgatcg tattgcaggt tttatgcatt
    33661 tttctcacta tgacttagaa gaagctcgta aaatacttgc agaatatggg atcacattaa
    33721 tcaattggga atatgcgcca ccgataagaa attttttcaa tgattattat tagtcttata
    33781 aaaaacagaa tgtaaaattt atgtgcgcga gtatttagtt ttccattttt tgtttgatag
    33841 atatatagca ttgttgaagc atgggatagt ggtgccaatt ttatcaatga ggttggtcag
    33901 gcagttaaca taattggtga tgctataaat ccatttaatt ggaattggag gaattaggtg
    33961 tggaaacagt gagattatgg atattcatga aaaattttcg ttcggctttg agatatgtta
    34021 ttctggaatc aaaatcaggt tattatctga ttgacacaga tcactatact ttttggggag
    34081 gctacctttt tccgattttt aattggataa tcccccataa ggttatgaaa attaacttgc
    34141 ctgcagaaga aataaataga ttaatactca acaaagattt acaaaggaaa agagagaaag
    34201 aacattctaa tgttgcttgg ataggtccag catttgcagt agtgacttct tcaattgtcg
    34261 gtcctatgct agagagtatt tatagtgatt tttctattgg aatacgttat ttatttgtta
    34321 ctttaagtat agctgttctt ttgtgcataa agg
//
LOCUS       DACDUU010000026        31198 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.026, whole genome shotgun sequence.
ACCESSION   DACDUU010000026 DACDUU010000000
VERSION     DACDUU010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.026"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(199..1971)
                     /locus_tag="IWL30_001262"
     CDS             complement(199..1971)
                     /locus_tag="IWL30_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9706039.1"
                     /translation="MRMQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1925..3679)
                     /locus_tag="IWL30_001263"
     CDS             complement(1925..3679)
                     /locus_tag="IWL30_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9706040.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLIKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3789..4358)
                     /locus_tag="IWL30_001264"
     CDS             complement(3789..4358)
                     /locus_tag="IWL30_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAP9706041.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(4376..5800)
                     /locus_tag="IWL30_001265"
     CDS             complement(4376..5800)
                     /locus_tag="IWL30_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9706042.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            complement(5802..6470)
                     /locus_tag="IWL30_001266"
     CDS             complement(5802..6470)
                     /locus_tag="IWL30_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP9706043.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(6600..7184)
                     /locus_tag="IWL30_001267"
     CDS             complement(6600..7184)
                     /locus_tag="IWL30_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9706044.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(7515..7748)
                     /locus_tag="IWL30_001268"
     CDS             complement(7515..7748)
                     /locus_tag="IWL30_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAP9706045.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(7763..8713)
                     /locus_tag="IWL30_001269"
     CDS             complement(7763..8713)
                     /locus_tag="IWL30_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP9706046.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(8710..9552)
                     /locus_tag="IWL30_001270"
     CDS             complement(8710..9552)
                     /locus_tag="IWL30_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP9706047.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(9554..10273)
                     /locus_tag="IWL30_001271"
     CDS             complement(9554..10273)
                     /locus_tag="IWL30_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP9706048.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            10965..11123
                     /locus_tag="IWL30_001272"
     CDS             10965..11123
                     /locus_tag="IWL30_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9706049.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            11195..12421
                     /locus_tag="IWL30_001273"
     CDS             11195..12421
                     /locus_tag="IWL30_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9706050.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(12509..12901)
                     /gene="rpsI"
                     /locus_tag="IWL30_001274"
     CDS             complement(12509..12901)
                     /gene="rpsI"
                     /locus_tag="IWL30_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP9706051.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(12917..13360)
                     /gene="rplM"
                     /locus_tag="IWL30_001275"
     CDS             complement(12917..13360)
                     /gene="rplM"
                     /locus_tag="IWL30_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAP9706052.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(13879..14346)
                     /locus_tag="IWL30_001276"
     CDS             complement(13879..14346)
                     /locus_tag="IWL30_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP9706053.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(14466..16004)
                     /locus_tag="IWL30_001277"
     CDS             complement(14466..16004)
                     /locus_tag="IWL30_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAP9706054.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            16084..16770
                     /locus_tag="IWL30_001278"
     CDS             16084..16770
                     /locus_tag="IWL30_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAP9706055.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(16971..17498)
                     /gene="msrA"
                     /locus_tag="IWL30_001279"
     CDS             complement(16971..17498)
                     /gene="msrA"
                     /locus_tag="IWL30_001279"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9706056.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(17582..18259)
                     /gene="pgmB"
                     /locus_tag="IWL30_001280"
     CDS             complement(17582..18259)
                     /gene="pgmB"
                     /locus_tag="IWL30_001280"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP9706057.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(18256..19386)
                     /locus_tag="IWL30_001281"
     CDS             complement(18256..19386)
                     /locus_tag="IWL30_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9706058.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(19386..21569)
                     /locus_tag="IWL30_001282"
     CDS             complement(19386..21569)
                     /locus_tag="IWL30_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP9706059.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(21566..22591)
                     /locus_tag="IWL30_001283"
     CDS             complement(21566..22591)
                     /locus_tag="IWL30_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9706060.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(22636..23463)
                     /locus_tag="IWL30_001284"
     CDS             complement(22636..23463)
                     /locus_tag="IWL30_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9706061.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(23460..24527)
                     /locus_tag="IWL30_001285"
     CDS             complement(23460..24527)
                     /locus_tag="IWL30_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAP9706062.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(24553..25389)
                     /locus_tag="IWL30_001286"
     CDS             complement(24553..25389)
                     /locus_tag="IWL30_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9706063.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(25386..26255)
                     /locus_tag="IWL30_001287"
     CDS             complement(25386..26255)
                     /locus_tag="IWL30_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9706064.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(26325..27608)
                     /locus_tag="IWL30_001288"
     CDS             complement(26325..27608)
                     /locus_tag="IWL30_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP9706065.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(27622..29307)
                     /locus_tag="IWL30_001289"
     CDS             complement(27622..29307)
                     /locus_tag="IWL30_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAP9706066.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            29582..30607
                     /locus_tag="IWL30_001290"
     CDS             29582..30607
                     /locus_tag="IWL30_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9706067.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
BASE COUNT         9532 a         6226 c         5074 g        10366 t
ORIGIN      
        1 tgtttaactg gtttatcgcc aattttttaa aactttgatt attccttata ttataagaat
       61 agacattaag tgaactaatt caatcatact gattctttcg tgtattcaaa attagatgct
      121 gtttaataaa attattcttt ttgaaaaaga attataacta tatcccaaaa attgtaggtt
      181 aatttaaagc ctatttcatc atcgtgattg agcttctttt tcatagacga atttctcaga
      241 tagtttagtt ttctttatga gatttttgta tatttctgac cttcgataca actcattatg
      301 agtaccttga tcaactagtt ttccttgatc catcactaca attttattta ccttttcaat
      361 agacttcaag cgatgcgata taattagtac tgtcttatct tgtattaaac gatttaaact
      421 ttcctgaata cgtttttcat tttccgcatc taaactggca gtaatttcat ctaaaattaa
      481 aattggcgca tttttcaaaa aagcccgtgc tatggatagg cgttgtcgct ctcctccaga
      541 aagtgaactt ccgttttctc cgaccaatgt atgataaccg tcaggtagtt tttcaataaa
      601 gtcttcgcag ttagctaaac gagctgcttt tttcacttcc tcatctgacg cttgactatt
      661 tccaatacgt atattttcca aaatacttgt attaaacaaa gttacctctt gaaaaacaat
      721 agcgattttg ctaaacaaag aagctggaga tacacgttta atatcatacc catcaattaa
      781 aatacagcct tcatcataat catacagtct agacactaat ttcaagatac tagttttccc
      841 ggaaccacta gcacctacta acgcagtcac ttctccttgt ttagccgtaa aagagatatg
      901 atctaaaatt ggggtattgt tatcatatga gaaagagaca tctcgcaact caatgtcaaa
      961 agatttcaga ggactatctg atccctcttg gatactggtt tccttcatag ctctaattcg
     1021 ctgtatttta ggagctaaat aaaatatttc aagcactgct tctttcatag aatcgaagga
     1081 atcctttatt tttattgcgg ctagtaagta accaacgacg tagagtatag tcacttcacc
     1141 cgttaacagt aagtgaacac ctactagaat gactactgct aaactaacat atgagaaaat
     1201 agaggataag gccatgactg aaaaagtact taattctacc tttaaatgaa tcctttcact
     1261 ctcttccatt tttttgaaaa gtctgtcttg aactttttta gatagattaa agctattaat
     1321 ctcttgctgc aattctatag tttcttgaaa ttcttccgag ttttctctca aagtatcgta
     1381 atatttagta ttggctttcg tttgggattt ctttgataac aagattaaga caaaactaaa
     1441 taacgttggc aaaataacag ctaatcccat tttgacatta ccaaccaaaa gcatcactga
     1501 aataaaaggg aaaaacagag ccataccgcc ggctttaggt attgcatgac tcatcgcatg
     1561 ctcaatacct tcaacgtcag acatgatagt ttgagataaa tctgataaat tatgttttga
     1621 aaaataggat aatggtaaat ttgacaaatc gtctgcaatt tgcactctta aatgcgcact
     1681 ttctttataa gtactattat ataagttttc atactcccta tctaacaacc aatacatgaa
     1741 aagcaaagta acaaacgata cggcaaaata aagccaacgg ggctttgtat tttctaaaac
     1801 taattcgtct atcagcatca ttataatgaa tgctggtacc atattaatgc agtatacaaa
     1861 aaaactagaa atagaagctt tagttaaagc ttctgcgccc tgatcactta aagcaaattt
     1921 gtttttataa aaacttttca ttgtcaagcc tccaatcatt tgcattctca taattagtga
     1981 ccaattgttt atacacttga tgtttagcca ttaattcatt atgatttcca cgttctacta
     2041 ttttcccttc tgacaagaca agaatttcat ctacagattt aatgctggat aatcgatgag
     2101 ctatcataat aactgttttt tcttttatca gatgttgaaa agccttttgt agttcatact
     2161 cattatctgg atcaatagat gcactggctt catccataat aacaatcttt gcatctttta
     2221 aaattgctct ggcaatagct atacgttgtt tttctcctcc tgacagataa actcctttac
     2281 taccaattaa ggtatgctct ctttcgggaa atttttctaa aatcgattcg caaccggcta
     2341 atcttagcgc gtcaaatact ttacttgctg gcgcttgagg atttgctata gaaacgttat
     2401 cgtaaatact ctgattgaat agtttcgtat cttgaaagac aaaagcaatt gtgttaataa
     2461 ttgtgtcttt tgaatagtga tttaaaggct taccaccaat tttaatctgt cccttatcca
     2521 cattgtagaa acctgaaatt agtttagcta tcgtactctt tccgcttcct gattttccta
     2581 ctaatgcata aattcttttt tgttctaaaa ctaagttgaa attttctaaa acacactctt
     2641 ctttgtatga aaagctgaca ttttcaaatg caatgtcaca atttttaatc tgtgtctcgt
     2701 tcccatattt tagttggtct tgcttcattt ctaaataaag ggagtctagt gtttcgactg
     2761 catatcttgc ttcaaacaag tgcatactta catacattat tctcatgaaa gaaacaaaaa
     2821 taattccact taaaaaaaac aacatcaaca agtcaacagc taatatcttg ggagtggtaa
     2881 gagaagttat aaaaaaggtt ataggaacta tcaaaatgca aactatgcca aaaaatagcc
     2941 attgaaaaag aacaaaaggt gttttcccac tcaaagaata cttatatgca aacttagcat
     3001 aatcgttaat tgctttattc aaggctttaa atgatgttac tttagcacca aatatcttta
     3061 taacttgaat tccgcggata tattctaccg tttcagagct taacacatct aacgattctt
     3121 gatatttttg aataaaattt gtattctctg tcattttctt taacaagaat acaccaatta
     3181 ctaacatggc gattagaacc aatccaattt taaagttaat gaaaaagcct aaggcaactg
     3241 ataaaacagg tgttactatg gcttgagcac tatctggaat caagtgagca attgccatat
     3301 gtgttttagc cgcgttgtca tcaattgtct ttcgaatata tcccgaagaa tttaaatcaa
     3361 aaaaacgaaa acttgcttct gttaaaccgc taattccctt ttttctcaaa ttagtctcta
     3421 agcgaaaccc aaggatatga gaaaataagc cagaaaaaat ataaaataaa gctcctaaag
     3481 ttaaccaagc aacagtttga accgcataac tcttagctaa atattcatct cctacaataa
     3541 ttagagactg aagaaatttg taaatactgt aatatccata gactgttaat acgacagaaa
     3601 aatcagaaaa taaagtggct aaccatccgt agtatttcat tttagggaca taactaaata
     3661 attttttgta tactttcatt ttttcctccc ccgcattcaa tttcttctct tattcgttta
     3721 caactgttaa tgctttccgt aatagcgtaa attagagatg tacaaacaga taaaaggtaa
     3781 actacgttct attttttgta tttttttaag acttttcttc caatcaatac accaatcata
     3841 gataaacttg ctacaactaa cactgaaatg attccccata tacctaccgc atagtggatc
     3901 attgcatcca ttgttgcagc agattgtcct ttatctatcc aatcttgacg aaaacgatct
     3961 gtaaagaacc atataggaat ccactgtccg gcagcactgc ctaattgcat tagagcccaa
     4021 gacgcaactt gaggaattgc tcgatcatat ccaaatacag atagaataat atcagctaag
     4081 atcgccatac taatcgaact gataaaccaa ggtaagtacg ccccgcccat taacgtaaaa
     4141 aataacaata aaacaattgt gtaaatagta aaaattcctt ttctagggta gttatagcta
     4201 atataaacaa atatgattcc agataataat ccaaaaatcc cgggggatat gctgtgacca
     4261 aacgccccac caatactact aaccattatt gaaaaaaagt aaattactag ggaaataact
     4321 gtagctaaac caataaaaat ataatctttt gttttcatat atattctcct caattctatt
     4381 tcgttatgtc attaaaaatc tgttctatca aagttgaatc ttctactcta agttttatat
     4441 ctttttttat tccaccattt tttaaataaa taattctatt gcatgtttta taaagaaatt
     4501 ccggatcatg agtagctata actactgcac ccttttcttt taagtcttta ataaggtcag
     4561 caatcctttc catattaata taatccaacc cacttgtagg ttcatcaaat agaaataaat
     4621 tagtttttga caatgaagct atagaaacca ataatctttg tttttctcca ccggataaat
     4681 caaacggtaa ttgatctttc attttataaa gttccatttt ttttaaaatt ttttcttgct
     4741 gatctttact aactatttca ttccccaggg ctaactcgct cctcactgat tctgtgaaaa
     4801 attggtaatc catttcttgc attactaaaa acggcgatga caaatttttt ttattttcga
     4861 ttatctttcc tttggtaggg cgaataatct tcatgagtat tcttagtaag gttgttttcc
     4921 ccacaccgtt tttccctacc aaaccaataa catctccaac tttgacatca aaatttatgt
     4981 cttttagaat attttcataa gtaatttctt caacttttaa tacatctgca cttacctttt
     5041 cgatataggg aacttcaatc gaactattaa aaatgtcaaa tttccttaat gcgttattgt
     5101 tttcagtttt taacaactga atagttgtag tttttacagt tctattttta acaaatagaa
     5161 cttgatcaat taattctttc aaatagtaaa aacgatgttc agctataaca atagtaaatc
     5221 ctttattttt taaatcacaa agtaagtcag caagtttctt agtcgctttg aagtccaaat
     5281 ttgaagacgg ttcatccaat ataattaatt tagagtttag cgataaacta catccaatag
     5341 aaattttctg ccgttcacca cttgatagcg aaaaaatgtt tttatctaat atatttgaaa
     5401 gatctaataa ttcagctaat tcattgattc ttttatccat atgctctttt gtaaatccaa
     5461 agttttccat cgaaaatact aactcactcg ttgtatttgt tgtaaagaat tgtcctctag
     5521 gatcttgact tacgtagcca atattttttg atatactttc ttgtgttaag gattgtagcg
     5581 agttattttc aaataaaatt tccccaacca attttccgtc ataaagtctt ggaataagtc
     5641 cattcattaa ctttaatagt gtggatttcc cactcccaga agaaccagta atcaatgtaa
     5701 gactgccttt ttttattgaa aaattaaggt caacaagcgc ttgttcctta acatccttat
     5761 atgaaaaaga aacattgtta aattcaaaca attctttcac ctcaaatcaa aatacttaag
     5821 aaaaaaatag taacggaaaa caggatgata tagtctaata agctcatgtt tgttttatag
     5881 taagaacttc ttttcacagg agattctatc ccctttgtta ttgcattagc ggtcatttct
     5941 tcagcaatca tagttgcacg tataagaacg ggaactaaca gaaattccaa atattgcaga
     6001 ggctgaaaaa aagacaagcg aatgtttctc atttttaaag attctttaat cattcttatt
     6061 tcagaacgat aagtcggaaa gaatctgatc actactgtaa tacttaacat tatttttttc
     6121 ggtaaatgta ttttttctaa agaggataat aattcattgc tagtatagac actaaccaac
     6181 aaccatgaga tcattacgac cggcaacatt cgaaatacaa tatataagga tgtcgcaaaa
     6241 aatatagtaa aaaactctat cttgatagca tcaataattt tcattactaa aaacagaact
     6301 gaataaatca agatgaaata aatacatgtc tttattttac gaaacatcaa tactaaaact
     6361 gtagcaataa tgaaacaccc cacttgaaat atatttccct ttcctaaaaa aataatgacc
     6421 ggaaaaaaaa tatttaaagt aatcaaggtt aacggattga tttttgacac tctttcacct
     6481 ccaaaaaata agatcgcact gttatcttaa gctgataaaa atgcaccgtt attcagataa
     6541 cagtgcgatc ttatcaaaaa gcataactga gaacaattct caatgcaagc tttttttaat
     6601 taactcagta attcaatcca cccaggaaca gaaaattgat acaatcttct ggttaatagc
     6661 atagtctttt cttttgaata gctatgcttc aatatatcat agaatgtagc gtagaatgag
     6721 taaatgtaga agtggatttc ttcttcatta aagactaaat tttgattgat cattccgata
     6781 gtctttaact tatctagaca acagactgtc tttttcgtca caaatgatgt aattttttca
     6841 acaatatctt gatatttggt tccagctgat tggaataaaa tcaaatcgaa tatacgttta
     6901 ttctcaaaaa tataattaat aaatggatct aattcattga agttctctct ccaacttttc
     6961 tctatatcta tattttctaa gtttaagagg tatttggtaa actcttcatt atacatatta
     7021 ataagcgcat taatatgcgg ctgaataatt tcttcaaata acgcttcctt agaagtaaaa
     7081 tgtttataaa aagcacccgt agttacgcct gcggtcaggc acagttgcct tatcttagtt
     7141 tgctgaaatc cttctttttc aaaatggctt agtccacttt ctattaactt ttggtgagtt
     7201 ataatataat ctctggtttc tatgggtact tcctcctaat aagatgaatt aactttatta
     7261 tcttacacag gacttgaagt ttcaatcgtt aacgtttatt actgaactca ttccttagag
     7321 ctaaactaat gagaaagagc agaataactg atacaaaaag caatcctaca agaattaata
     7381 gactcatcta aatcattcac cttttctaaa ataatccgag aaagatttaa aaaaattata
     7441 tccagctatt aggataagtc ctatcataat cactccaata gccattttct tatcctccct
     7501 aaaaataaag tcttttactt aaaacctttt ttgtaatcgt caataaattt tttcccttta
     7561 tcagttaatt gaaatcctat atctaaatag tcctcatatc taagtttagc cagcatctca
     7621 cttacagatg ctggactaac acttagaact tttgctaaat cattattttt taatttcttt
     7681 tttctttttg ataattcctg aattgctagt aaataaatta gtatagtagt cgttttaatt
     7741 tgtaccatac ctaccctcca aattatttcg aaaggccttc agcaatttta tccaggttcc
     7801 atttcatcat cgcatagtaa ctatcaccat cttgtccttt ttctgcaatt gaatcagtaa
     7861 aaatcgttga atagatagga atattggtat cttttgatac tgttttcatt ggtctatcat
     7921 ccacactgct ttctacgaat aaggagggaa cttttgttgt gcgtaatttt tcaactaagt
     7981 gttttatttg atctggtgtt ccttcttctt cagtgttgat ttcccaaatg taagcagaag
     8041 gcacattata cgctttcgag aaatatttaa agcatccttc acttgtaaca atcatttttt
     8101 tatcattcgg aattgaagca aatttagatt tagcttcttt gtctagagaa tccaattttt
     8161 caatatactt atctagattt tctttataga attttttatt atcaggatct ttttccgcta
     8221 actgtttttc aatattttta gcgtaaataa taccgttttc taaatttaac caagcatgag
     8281 gatcttcctt ccctttctca ctctgaccct ctaagtaaat aacatctatg ccatcacttg
     8341 ctgcaaaata gtctttgttt tcctctttgt tcgcattttt aactaatttt gtaaaccaag
     8401 catttcctcc agtttccaag ttaacaccgt tataaaaaat caaatctgct tttgaagttt
     8461 tttgaacatc ttcaggcaaa ggttcatatt cgtggggatc tttcccaata ggtacgatac
     8521 tgtgtaaatc aattttatct tttgctatat tttcggtaat atctgctaaa atcgaattag
     8581 taactactac ttttaatttt tcgttcgaag ccactttatc tttgtcttta ctatttattg
     8641 aacatgcccc gaatataaaa agagcaatca aaatccccag acttcccagt actgctttcc
     8701 attttctcat cataacgatc tctccttctt tcttaaaaat ttttgttttg gtgagattag
     8761 aaaactaatt acaaataaga atgccgctgt taaaacaata cttgatcctg ctgctatgtt
     8821 aaaactataa ccaataaaca atccaagcaa tgatgaactt gctccgactg ttgcagaaat
     8881 aaatatcata gttttcaagc tatttgcata taaatatgct gtagctgctg gtgtaattaa
     8941 cattgcaaca attaatatag ttcccacact ttgcatagct gttactgcta ctaaagttaa
     9001 tagaaccatc aatagataat gataaaaact gacattcatc cctattgctt ttgccattaa
     9061 tggatcaaaa gaagtgatta ataattcttt aaaaaataga actactataa ccaacactaa
     9121 aaccgaaacg ccaattgtaa tccacttatc aatatcttgt acagctaaaa tatttccaaa
     9181 taaaatatga aataaatccg ttgagctatt tgcaactcct ataaggataa ctcctagtgc
     9241 taaaaaagaa ctgaaagtaa taccaattgc tgtatctcct tttattacac tattgctttt
     9301 tataaaagta ataatgatag aagctaatag accaaataca atcgcaccaa taaaaaaatt
     9361 aatacctaaa atataagaaa ttgctactcc tggcaaaaca gcatgagaaa tagcatcacc
     9421 cataagagac attcctctta aaataataaa acaacctact gttccagaaa caatccctat
     9481 aactacagat gtaattagag cattctgtaa aaagtgaaag tcatacagac cattaataaa
     9541 attttgaagc atattattcc ccctctccaa taaatatagt atctccatag gcttttttta
     9601 aattttcttc attaaataca gattctacac ttccgtgagc aattagattt cgattcaaaa
     9661 gaataatatt atcaaaatac ttctttacct tgcttaaatc atgatgcaca attagcactg
     9721 tttttccctg atgtttcaat ttttttaacg tatccataat tattcgttca ctaactaagt
     9781 ctatccctac aaatggttca tctaaaaata tgaaatccgc attttgagca agacatcttg
     9841 ctaaaagtac acgttgaaat tgtcctccag ataattctcc tatttgatga ttagaatatt
     9901 ttcccatatc gaccttttcc aaagcctttg aaactctttg ccattctgca tttttaattc
     9961 tttgaaaaac tttcattccg gcatatgttc ccatagatac acattctttt acttttattg
    10021 gaaaagtaaa atcaatatca gctttttgct ctacatatgc aatcttcacc agttttttat
    10081 tgatatcttc attattaatg gagacctctc cctgatgagg gataatattt aacattgctt
    10141 taagcaatgt ggattttcca gcaccatttg gaccaataat acctgttaaa gcaccttctt
    10201 gtatttttaa agaaatctcg ttcaatgcta aaaaatcgtt ataacaaaca ttaagctttt
    10261 tcacttctaa cataactttc tccttctaaa tgataatgat tatcacgatc atatggtata
    10321 caaaaattat attttagtca accctaaatt ttatagttta gatttttttc tgttgctgaa
    10381 gattagaatg acaaaactat ttgaaactac gcgtatagta agtagctttc aaaatatata
    10441 aaaaaggagc gttctcaaat gagaacatgc tcctaaaact actttattgg cgtatggaaa
    10501 aattatactt gaagtggata gcgggttgaa taagttgata agcatcaaaa catttataaa
    10561 aattattcaa gactatgtct gttccatata caaatatgaa aatcagtaaa ctccatttaa
    10621 cttttttcgt agtgtgtctt tttatacatg tttatcgtta ttaatagtga ataggaaaaa
    10681 aactattttt gatcattgac aattaaatac aatttttgaa taacgatccc tgctattcga
    10741 gctagaaact caagtacgcc atgacctgat tagcgacaaa tacttagaga tttctttttg
    10801 ttctgggaaa acaaaatgcc atgacaatag cagcaaataa tgatacttcc gtaaggctcg
    10861 ccgtaaactg gggagaggtg aaattcctat gtgcttaccc aaagcaattc aaaaatttca
    10921 taactactat tttaggagat gattttatag aatccagagt ctacctgaat aaaaaacaaa
    10981 cctgtgcatt tctaggaatc gataataata ctctagataa gtggattcgt atatataatt
    11041 tcccagtaat aagagttggt ggtgtagttc gctttgacgt agaagatatt aaagacttta
    11101 tgaatacatt taaaataact tgaaattttc ttctcgccgc atatactaaa attagtgaaa
    11161 tatactgcct tacttttttt aaggagtgag gattttgcct acaaagcttt caaatgggaa
    11221 atataaaact aatctgagat atccaaaaag gtttaaagaa ataacaggaa ttgcatcaga
    11281 aaagtatcaa aaaacatttc caaataggca attggcgatt aaagccgaaa atgatatgaa
    11341 gaagaaaata gaaaaagttt tgagagagga gaatgcaaac tcattagaat taaaaggaaa
    11401 aataacgttt aaaaaatttt acgaatctaa atggcttcct agatatgaat taggtcaaac
    11461 aattagatca aacaggcctc ctagtgatat aaccattagt aatacaaaag atatatttcg
    11521 gttgcatatt ttacctatgt ttggtgaata cgcaatgaat tatctaaata tcaatactga
    11581 gataatatca gatgaattaa ccaaaaaatc gaaagaatat gcaaatataa aaatcataaa
    11641 aggatatgtt cgttccatgt tcgatattgc agaaatacta aattatatcg aatttaatcg
    11701 aactactaaa attattcaaa gtatcactgc acctaaaaaa aatgctttag aagaaaaaag
    11761 aatacaagaa ggtaagcaag cactatcatc aaaagaactt actaattgga tagaagcagt
    11821 aaatgatgat tttaataatc atttacttac ttttcatgat tacacacttt ttatgattac
    11881 cctgtatctt ggtgatagaa aaagtgagac ttacgctttg caatggaaat atattgattt
    11941 tgaaaaacaa acggtccgtc ttaaacatac tttagataaa tatcaaagaa aaaagtttac
    12001 aaaaggtcga aaagataccg tcattcaagt accagaagtt gtaatgacat tgctaagtga
    12061 atggaaatcg gtacaagctg atcaattgtt gaaattaaaa attaagcaaa cgttagatca
    12121 gtatctcttt acttatacta aaccatctgg agaagtaaat tgtcctgttc atgccgatta
    12181 cctaaactat cgaatcaatt caattaaaag aaggcatcct gacttagttc atttatctcc
    12241 acacaaactc cgtcatactt atgcaactat agctagacaa ggtggagcag atatgaatca
    12301 aatttcaaat gctttaacac actcagatat ttctacaaca aagatatatg tgaacacacc
    12361 agatatagtt gataaggctg tttttgaagc ttttcagaga ggattgaata agtgtgattg
    12421 atatgtgttc ttaaatgtgt tcttaaaaac aaaagaagca ctttccaaat aattgaaaag
    12481 tgccagaaac gttgatatat aaaggatatt aacgttttga gaattgtgaa gctttacgtg
    12541 ctttcttaag acctggtttt ttacgttcga ccatacgtgc gtcacgagta agtaagccag
    12601 cacgtttaag agctaaacgg aagtcaggat caacttctag caatgcacga gcgataccat
    12661 gacggatagc tcctgattgt ccagcgtatc ctccgccgtt tacgttaaca aatacgtcat
    12721 atgcgccttt tgtttcagta acagcgaaag gttgattgat tacttcacgc aagtcagcat
    12781 gtggaatgta ttcttcaaca tcttttttat ttacagtaat tttgccagtt cctggtacta
    12841 gacgtacgcg agcaactgcg tttttacgac ggcctgtgcc gatatattga acttgtgcca
    12901 atgaaattcc ctcctattag attaagtttg tgatgtctaa aacttctggt tgttgtgctg
    12961 catgtggatg ttctgctcca ccgtatacgt tcaattttgt aaattgtttg cggcctaaag
    13021 tgtttttagg aagcatacct tttacagaag tttcgatcaa acgacgagaa tttttagcac
    13081 gcaattcgcc agcagtgatt gatttcaatc ctcctgggta gttgctgtga cggtaataaa
    13141 ttttgtcagt cgctttttta cctgttaatt tcactttgtc tgcattgatg acgatgacga
    13201 aatcgccagt atccaaatga ggtgtgaaag ttggtttatt tttaccacgt agtacagatg
    13261 caacaactgt tgaaagacgt cccaatggaa catcagttgc gtctactaca taccatttac
    13321 gttctacttc gcctggtttg gccatatatg ttgtacgcac gttattttcc tccaatttaa
    13381 gttctgatgt ttgacataca cttcgagttt ccggggctct ttgtggggca aacaatacca
    13441 tttaacatat tacccgctaa tctagtcaat gtcaacgttt ttctgcttga ttgaaaaatt
    13501 tatatgaaac tttacttctt ttctttcttt tatataaaag ggtcgtttcc tgtcaaaatg
    13561 aaaaacaaaa agaggctgtg acaaaagtct tgtcacaacc ccttattttg aataaacggt
    13621 gttctcctgc caactcctcg gtcacaagtc acaatatcca taaaacataa gaagctgttt
    13681 tttaaaattg tttcttgtga ctcggagctg aacggcaggc ttacaacctc ttggtaaacg
    13741 gtgttcaatc agctgaccct cggtattagc tcaaaaaacg gcaaaatatg aggaactatt
    13801 ttgacgtttg tttctctaat actcaggtca aaacgctgat ttcacaacct ctttttcggt
    13861 caagttcaac aacagttttt acgcgtcgat ctctggcgct ttgcccaaat gagcttggat
    13921 catccagata cgtttttctg tggctgtttt gaattcgata aagatatctt gtgttggatc
    13981 gtcgccttct tctccagaaa cttcgattcc tttttggtaa agatctgcta gataacgata
    14041 gcctgcaaca agtgtttcca aacgttctgg aatagtacga tcccagcggc caatttcatc
    14101 agggattttt gtgttatctg caaattcacg caatgtagaa taaggtgaac cgtctaatgt
    14161 gatcaagcgt tcggatactt catctaactg agcgttcaaa tcatccatga actcatccat
    14221 cattgggtgc aatgtaagga aacctggtcc tctcatgtac caatgtgttt ggtgaacaac
    14281 aacagacatt tggcttagat ctgcaactaa ttgatttaat acttctttgg tttgttggaa
    14341 tttcatcttt cttgcctcct aacattttct tacacttaaa ataatagtag actttcaatg
    14401 aaaagtacaa caaaatcatt tacctgagaa cgattttcag ttcgcttttt tatccgtaaa
    14461 gccaatcatt tttcagaatc agctaattct tctcgcagtt gagcaaattg ctgataagtc
    14521 tgtgctaaat gcttttgtgt cgaaacaaat aatggataga ttttctgata ttttttatga
    14581 tcagtttctt gtactccaac ttgctctttc aattctatca attcctgtgc ttcactgata
    14641 ctttggatct ttccgatgct tctccagcct aaaatcacag ctcctaatgc ggatgcttct
    14701 gtgacagtag ggaaaacaag cgggtaaccc agtacatcag ccatcatctg acgaaaaatc
    14761 ggagattgag caaacccgcc tgtagcacgg atctctgtga gctgattttc ctggcaaact
    14821 ccttttaaaa tccgataaag gttgagacag atcccttcta gaatcgcctt catcatttct
    14881 ttttctgtat gttgccgctg taaaccgata aaactgccaa acgcatctgc gtcccaaagc
    14941 ggtgctcgct caccaagtaa atagggatga aaaatcagac cattggcacc cgctggcaca
    15001 aaagcgatag ccgaaagcag tgcatcatat ggattttctc ctttttcttt agcagattga
    15061 acgacttcct gtaaaaatgt atgccctgcc caatcaaaga tgccagcacc attgctagtc
    15121 gctcctccca ctacccaatg tttctgatcc agaacataac aaaacgtctc catttctgga
    15181 tggaactgag gctgagtagt aataaaacga agtgctccgc tcgttcctac ggtcaaagcg
    15241 gcgatcgtat caccaaaagc acctaaccct agattagaaa gcggcccgtc tgcccctccc
    15301 aaaacaaatg atatatcatt tggcaaacct agttctgctg ctgtttttga tggtagacct
    15361 gttacttgat aggtggaagg aaagacttgc ggcaattgct ctattcgaat gttcaagtag
    15421 tcaagcactt cttctgccca ttggaattca tggatatcaa aatatccagt tcctgaagct
    15481 gttccgtagt cgctgatcag ttcgccaaac aatctataaa agatatagtc tttgataccg
    15541 acaaaataag cagttttttt gaataattca ggttggctgt tttgcagcca cttgattttg
    15601 ttcaatggtg tcattggatg aatcggcgtc ccagtctgcc gataaaattc tttgccaagc
    15661 acggtatatt tcagtttttc tgcttcgtcc gctgctcggt tatctgccca agtgatcatt
    15721 cgagataacg gtgttttctc ttcatccaac aatatcaggc catgcatcgc agaggagaag
    15781 gagacaaatt gaatgattgt gtctgaaggc aactgtttaa ttacttcttt tataacggtg
    15841 cagacacctt gaaagatcaa ttcctgatct tgttctgcca tcccttgtgt ttcttggatc
    15901 aaagggtagc gaaaataagc ggatgctgct tcttgggctt catctgtgaa agcaactgct
    15961 ttggtttgtg tggttccgat atctaatccg attgaaacgt tcattttttc acctcaaatt
    16021 ttatgtatgt acaaaaattt agtttaagct tgcgtatctt ggttttgaaa agggggagat
    16081 ggcgtgttac tttattttat cattggttgc tgtatcggat catttttatg tttaaccgcc
    16141 cagcggattc cgttaggtca ctctattatt tatcctagat ctcattgtgt caaatgctgt
    16201 cgttcgcttt cttggtatga attgattccg attctctcga tcatcgtcca acgatttcgc
    16261 tgccgctatt gcaggtgtcg gttgcccttc tactatttgt tggcagaagc cttatgtggt
    16321 ggtctttttg cctggttctt tactttttct tccgcaagaa atttttcgac gttcatatgg
    16381 atgctttcgg ctttgctatt ttcgttgatg gatttatttt acttcatggt ccattctcac
    16441 acgctattct gttcttgggc aattttatgg attttttggt tgcaggccgg cgtcttccaa
    16501 tggcaaagtg tgctgttaac gtttatcgta ggcggtgtgt gccttcgcta tggtcaatcc
    16561 tatctcggag ctggcgatac tctgttgatg ctttcttgga gtggtggctt gtctctagaa
    16621 gagttgcttc agatcttgtt tcttgctagt ctgtttggaa tatccgtttt ttctgtttac
    16681 tttatctgtc ataaaaaaaa gttggaaaag ctgcctttca ttcctttttt gagcagtgcg
    16741 ttgctgattg tccttcatct taggtattga aaaaaggttg tgacaaacga cttgtcacaa
    16801 cctctgatat atagtgttct cttaccaaat ccttgtgact tggagctagg cgacaggttc
    16861 acaacctctc cactaaatgg tgttcaatca gctgaccctc agtattagct caaaatgctg
    16921 gttttacaac ctctgatata cggtgttcca taagtagctt tttcggcttc ttatttcttg
    16981 acgccccaat gtcgacgaag gaaacctgca cgtccagaac cttctcgata aagattgtaa
    17041 tggagcttgt ttttcttgta ataatcctga tggtaatctt ctgcaggata aaattcgctt
    17101 cttggttcga tttttgtcac gattggctta tcgaatcgcc cgctgtcttg caatgcttgt
    17161 tttgaacgct ctgcaatatt tcgttcttct tcatcattat agtagatcac tggacgataa
    17221 gaatcaccgc gatcagcgaa ctgtcctaat gcgtcagtcg gatcggtttg ctgccaatag
    17281 atttccacca actgatcata agaaataact tctggatcat attcgatctc tactgcttct
    17341 gtatgccctg ttgtgcctgt tgttacttgt tcataagttg gatttggtac atgcccgcca
    17401 gtataaccgg atgtcacaga aatgatccct ggctgctctt caaatggttt gaccatacac
    17461 caaaagcatc cgcctgcgaa aactgctttt tttgtcatat aaaatccccc ttttttctca
    17521 tcataccttg atttccaaaa ggaggataac gttttgcttg tgacagaaaa aacggaaact
    17581 attatatacg tgatacggct tctgataaat gccaaaacga acgtacttga tcaaaaacgg
    17641 ttaaatcagg aatcacatat tcagcagaag gaagatttat gttttcacct attcccaata
    17701 catgcatgtt accagccaaa gctgcttgac aaccagcttc tgaatcttca atgaccaagc
    17761 atgcatttgg cggtacattc aattgctgtg ctcctttgag aaaaacttct ggatcaggtt
    17821 ttgcttttga tacttgcgtg ccgtcaataa ggacatcaaa ataacttgtc aaattcgttt
    17881 tttctaaaat gaggcgtgca tttttgcttg ctgatcccaa tccgatcttg atgtttgttt
    17941 ttttcaagta ctcaagaaca gctattgcac caggcaaaac ggatgtttca tccatttttt
    18001 gaatcgcttg aagatataga ttgttttttt gctcagcaaa ggctgatttc tctttttctg
    18061 agaatttctg ttccattttt ccaaatgaca gcaatcgctc tagtgactcg gttctggata
    18121 ttcctttcag ttgctcgttt tctttctcag tgaatggaat ggacagctgg ttagctaaaa
    18181 ccagccacgc ttcgtaatga taacgagctg tgtctacaag aacgccatct aaatcgaata
    18241 gtgctgcttt gattttcatc gattacctcc ttgaaaatga acgacttgaa gtaaagatgc
    18301 gatgttttta gctgtgcgat gaagattatc agtggcttgc tgtaaagctt cttgtaaagg
    18361 cacagctgca ggaaggatcg gaaaaacggc cgaaatgcca acatctgctg ctttttcgct
    18421 tcctttacca acacttccgc aaatagctat cacaggaacg ccctctggta ctcttctagc
    18481 aatcccaact ggcgcttttc cttttagact ttgtccatct agacgtcctt cgcctacgat
    18541 tacgaggtct gcttgctgta cccgtttatc gaactccagt aaatccaata cgtagtcgat
    18601 gccagagact attttccctg aagcaaatgc taacagtcct gcacccattc caccaccagc
    18661 actactgccc gatgcctcga ttacttgagg ggcaaatctc atataaaaat catgcatggc
    18721 ttggtcagcc actgctaatt cattttcttt caagcctttt tgtggtccga aaacaaaaga
    18781 agcgccttct tttccgcata aaagattagt aacatcagta atgacagtga ttttggcttt
    18841 aatcagctca ataggcacgt tcgttttatc aactgtagca attttaccta gattttctcc
    18901 cagtgcttct actttttgat tatcttgatc gtaaaaagca taacctagac cttctgccat
    18961 gccgatgccg ccatcgttgg tactacttcc gccaacgccg atgatgatgt gtcgaacacc
    19021 ttgttgcaac aattgtcgta tcagttctcc tactcctcgc gtggataacc taagtggctg
    19081 ttgttggttt agtgggacat ctttcaatcc gacgatttct gccatttcaa aaatcgcagt
    19141 ttgaccgtct gaagcgtact ggatttcttt gtttttgcca aatgcattag taacgactgc
    19201 tgtttgccgc ttcaacctta aactgtctgc taatgatgaa accgttcctt ctcctccatc
    19261 gcctataggt agcagatcat attcagcctg aacaaaaact tcttgaaaac ctgattgaat
    19321 ggcttgtgct gcctctaagg ctgtcagact ttctttgaaa gaatctggcg caatcagaat
    19381 acgcattatt ttcccctccg ttccaagatc agttcttttt ccagctggta ttggtctcca
    19441 ttgacaaaaa taggtaatgt ttctttcgtc tcttttttta ttactatttg tttttttgtc
    19501 aaaacaaata gcaacctgtg atttttccaa ataaaaggaa aagaaatttt ttcccatgct
    19561 gaaggaagtc ttggagaaat agaaagagtt ccattcgtta ctgtaatacc tgaaaaacca
    19621 aatataactg ctagccatag tgctcctaat gaagcagcat gaattccgtc atctgatgaa
    19681 tgcggatctg gtcctaaatc aatcaggcac gcttcttgga ataaatgata cgcccattcc
    19741 atttcttgga tacggcaagc ctctatggcg tgaatcgctt tactcaacga tgagtcgtgg
    19801 attgttttgt tttcatagta atgcaaattc ttagaaacga ttggttgaga aaaatactca
    19861 ggtaataagt ataacagcat gacaagatct gcttgcttgc tgacttggag ttcatttact
    19921 tcgctacggg cataatcaag taaaatagac tggctgccag gacgttcttt gtaagtggtc
    19981 aaatcaataa ctggcttact gagatacgtg tcatcttgcg gaatcagctg ttctttgtta
    20041 ggcactggca aatataactt atctaaaaaa ttttgacatt ctttcaaaaa aagcggatcg
    20101 ccagcttgat atttaacagc taaggccaca ttataatgcg ccagatagtt cgtatatgca
    20161 ttattgtcga tatattctgt gtattcatct ggaccaatga ctctgtgtat ctctaaatga
    20221 tcatcgactt ttacagctct gctcagccaa aaagctgctg tttcatgaat gagcgttttt
    20281 ccctttgttt ctaaaaacga ttgatcctct gttgcttgga catactcttg aacggcataa
    20341 gcaaggtctg cactgatatg atgttctgcg gcagctgaag caacttcttg tcttgttccg
    20401 gttttgatat tcaatgcagc aaatttgggt gtctcttcct gaccagtgtg tgcgctttcc
    20461 catgggaaaa gtgcaccttg atagtgataa tctcttgctt tttgccgtgc gccttctaat
    20521 cgatcataac ggtacagcag caaattcttt gctacttcag gaaactgata taaaaagaaa
    20581 ggcaggataa aaatttccgt atcccagaaa acatgccctt tataaccttc gccagttagt
    20641 cccttcgccc ctacactaac acttggatca ttgcggggag tcataatctg taaatggtaa
    20701 gcagcgaagt ccatcgctaa ctgatcgaaa gaagaatcgc cttggatcgt tatccgatgc
    20761 tgatgccaaa aatctgtcca ataagacatc gactgctcta aaatttctgt ataactaacg
    20821 cttttctggg cttgggcaat acttttctta aacgggtctg tttgctgaag agaggtagag
    20881 agcacgatga tcttctccag cgtaaaggac gtattttttc tgatcttttc tgaaaaggaa
    20941 gcggtgatcc ggcgattttt tgcttggaat acacctgctt tattgaattt agctgcaatc
    21001 ccaatatcaa ttttactttc tgttgtctgg taggatgcac tgatcaaatc gtcaccaaat
    21061 actcgcagtt ctttttcaac caactgctgt gtaccaaaat tggtttgctg tccatcgata
    21121 cccgttttca catctacttg gcagtcttcg gtcaacggct tgatgaccac tttcagggct
    21181 gcctgatgga gattattttg atcagcaaca cgacaagtaa tgacttccac tttttttcct
    21241 tgagcagttt caatgagcgc atagcgttta agctctccca tttctaaatc tagtgcccgc
    21301 tcgtatgcaa gaactttcat cgtctcaaga gaaaaatgtt caccgtcgat ttttatttcc
    21361 catctgatga catctgggag attcactaac tcggctgatt ctgctcctaa aggtcggtta
    21421 tatacaccag ctgcaaacat tccccttact tgattccgat agtcttcctc ctggcttcct
    21481 ctgataccaa aaaatccatt tccaatggtc atctgactcg catacttatt gatatactct
    21541 aaatcaaact gtttttcttc tactttcata aggtaataac ttctcctgtt tctgccgaac
    21601 gatataaatt ttccaccaga ttctggatga catatccttg ccagccatct gcaatcatct
    21661 gagtctggtc accgagcgcc cgattcacaa aatgagccat acttagttca tgtgggtcat
    21721 taactggcgt ggtcttttgt aaggaaacca gctttccatc tacatcttcg aatagttcgc
    21781 ctggatacag attagcacct gcctgatcgc cacaaaaagc cacattcagt ttgtcttctc
    21841 tgatattcaa agcaaacgaa gtattcagtt ggatgatctt gccgttttct aattccagtg
    21901 tgccaaaaat tgcatcttct actgtgaatt tgcttggatc ccatttcccg aatttccctg
    21961 aatttttttt cgtaccaatt ttttggaagc tgtacgcccg aacgcttttt actttaggaa
    22021 atccagtaat atacatcgct gtatcaagca tgtgaatgcc ataatcgatc aaaggcccgc
    22081 ctccttgaag ttctttgtcg ataaacgttc cccagcctgg aacaccagac cgacgaagcg
    22141 cattcgcttc tacgtaataa atatccccga gaacatcaag atgattcttg atcattgttg
    22201 cttcgtcaga aaaacgatgc tggaatccat aagccaaaat ctgcccgttt ttatcggctg
    22261 tctctgccat ctttcttgct tgatcagctg tcatagctgg tggtttctcg cacatcactg
    22321 aagcattagc ttcgagggca gccaatacat gttcgaagtg aaagcgattg ggtgtacaga
    22381 cactcacgat atcgggagaa atctcattca gcatttgtgt aactgatgta tacgcatgag
    22441 aaaatcctgt acgtcggcaa aagtcttctg ctcgctggct gactggatca accacagcgg
    22501 ccaattctaa gacatctgat cttttttgat aataagcagc atgtactttt tctgctactt
    22561 gtccagcgcc tatgattgct actttcttca tttatttctc cctcctttgc ttatttactt
    22621 attgttattc tttctctatt ctttctctaa atcatttaaa tgacgcgctg tttccagaca
    22681 gtttttcaag tattgcaatg attcctgata aattttcact tcattttctc ctcgcaaacg
    22741 acattcatat accatataac cttcatactc attttgaagt agcgtttgaa aatggcgagt
    22801 aaaatcaatt gcgccagatc ctggttggta gcgatgatta tctgctaaat ggatatgacc
    22861 cacatactct ttttgcgtag tcaatgcttt tgaaatatca tcttcttcaa tattcatatg
    22921 ataaaaatca gcgatgatct gcacatgttt caattgatta gagtcaatgt aatgacgcgc
    22981 atccgctaac aaattgatca tatggtcttg gtatcggttc aaaggctcca aataaataac
    23041 cgtacctgtt tcttcggcta ctttatctaa ataacgaagt gattcgctga ctgctttata
    23101 gtctccttct ttgcttcggg gtgaaaccat cggcggtaat cgataagtga acattcccca
    23161 agctgctggg ataacgattc ctttgcctcc aacctcgctt agtgcacgca aaatctcagc
    23221 aatttgacgt acaccattaa gacgccgttc ttcgataaag tcaccgatcc acccatcgta
    23281 gccgttacaa gctgtcacta ctgggatccc tgtttctttg attgcctgtt tgactgtgtc
    23341 gatattttct attaacaact taccgtcgat ttcgtattct tcaaatccac agtctttgat
    23401 aaatctaaat ttttctaaaa tgtctcttgg gaaaaatgct tgatcttgcg taccgaattt
    23461 cattgttctc ctcctcggac ttgaacgccc attttgatac tttcttctgg gtgctggtct
    23521 acataacgca tgtaagcttc tgcgctttct gcaaatggca caactggatc aatgatttct
    23581 tcacaatcca agtaaccgtt catcaataat tcccaacatg tgtcctcgat tcgtttacgg
    23641 ttccatcttg ggtaatctgg gtttggttca ctagatgctc gagcaaagac gatttttgca
    23701 ttattgaaat gtgcttcgcg accaagatta aacccagctg gaaaaggttt agcaaaggca
    23761 acataagaaa tgatcccgcc atagccgatc cctcttaaag aagcttgtaa tgcggcagca
    23821 ctgccactcg tttcaataac tgaatctact ccacgtttat ctgtcaattc ctttagacga
    23881 aaaccgacat ctgctttcat aggatctagc gtgtaatcgg caccgtattt ttctgctaat
    23941 tctctacgct gcgctatggg atcgatccca acgatggtag tagcacctgc ttttttagct
    24001 aattgaatcg caatttgccc gatagcgcct aaaccgaaga ttgctacagc atcacctgct
    24061 cgaacatgtg cttcccggat accacctaaa gcgaattgag caggatcgta acatactgca
    24121 tttttccatt gatccaaccg atcaagttta cgtagtttat aattatcaac accgtttgta
    24181 atcagcgttt cacgaatggg tccataagtg cagactaact ctcctttttg atactctgtc
    24241 accaaactgc ccgtttcttc gatcgtaccg acaatcatat ttcctaattg aaattcgcca
    24301 aataccacac cgcgttggct tccttcttct cttggcaaga ataggcgcca ttcttctgaa
    24361 aattcttcat cgataaatgg acttaatcct ctaaagtcga caatttctgt tcctggtttt
    24421 ggagtagcga aaattactcg gatacgtact tcgttctttt cgatctcacg gtcttcgtaa
    24481 tctttgaatc cggctattct tggttctaat gcaactaatt ttttcatctt ttctttcctc
    24541 tttcttttct ttttatcctt ttacgccgcc ttcgactaaa tcacctttaa taaatcgttc
    24601 agataacgca tacataatga tgactggtaa agcggtcacc aatgaagcgg ccatcattcg
    24661 tccccaaatg tagtctggtg tactgaataa tgtgttcaaa ccgattggta aagtaaattt
    24721 ttcggaggtc gacaggaaaa tcgaagcaaa taaataatca ttccacgaaa ccataaagca
    24781 ataaacgaag acagaaatga ttccagaaat agccaatggt ataatgatcc gaataatgat
    24841 ctggatccgg ctaagaccat cgatcattgc tgcttcttca atatcagtcg gaatcgtgtc
    24901 aaaataactt tttagcatga atatcgcagt aggaagtgtt tgaacgacca tcgtgataat
    24961 cagtgctgta ttggtatcat acaaacctag accggaaatg attttaaata atggaacaac
    25021 gatcaaaata ccggaaaaca tataaacggt gtaaaagcta gcattgattt tcgagcgagc
    25081 tttgaagcgt agttttgaca aagcgtatgc cccgctgatc ccaatcacta ctgcagttac
    25141 ggaggctatc aaggagatca ctaaactatt tttgaaatac atgatgaatg gaaaaatctc
    25201 agcattgaaa atatcctgat aatgttcaaa agtccattct ttcggaaaaa tagtttgtgt
    25261 cgttgcaatt gcttcttgtg aatccttgaa tgatgtcatc agcatgatca aaaatggaaa
    25321 caaattgaca gctaatagca acaaaatcgt cgcgtaaaat aagattttcc tgctcttttt
    25381 atttttcatt tcaagtccac cttctttcgt gagagcaaga cgaccgctgc aataatgatg
    25441 aacaatagaa tcgatatagc tgctgctttt ccaagatcat tgaacgcaaa ggctgtctga
    25501 tacaaataaa cgcctaagat attgacttta ttcgtcagta gatagacatc tgtaaacata
    25561 tagaacatcc agatagatcg taaaacaatc acagttgcta gtactggttt gattgccggc
    25621 agtgtcacga tcttgaactt ctgccaagca tttgcaccgt ccatttcagc tgcttcataa
    25681 agcgttttgt cgactgtttg caaaatagcc aaaaatgaaa taaacgcata cggatagtat
    25741 ctccagatag caaacaaaac gaccataaag aagctagcaa ctggctggtc gaaccacagc
    25801 ggtgcttcgt tgaaaagctg caacctatct actaacagat cattgacgat tccataacta
    25861 ttattgaaca tatatttcca agcaaaaatc aaagaaatag atggcgtaac ataacttaat
    25921 aaaatcagtg aacgcaccgt tttcctaaat ctgaacttgc ggttaagcaa aatagctacg
    25981 gctaatccta aagctgtact gcctgctaca actaagaatg tgtaagcaac tgttaaacct
    26041 aaagaacgat aaaattcact atcacttaaa atttcaagat aattgcttaa tccgataaaa
    26101 acggcgtctg agttaggatt cagcggcata tcgaaaaaac tgatctgaat attggaaagc
    26161 attggaatca gcactaaaac agctaataaa agcatgcttg gtgctaaaag tcccatggct
    26221 aattttaagt cagaatgttt ccttggacga ttcattgaaa cctccccctg ataatctgcc
    26281 aatagctatc tacttttttc gaacaccatt ggcagaactt cttgttattt cgtatttttt
    26341 tgtgcgtcag ctaaagcttg atcgacatct tcgtttccga cagttacttg gttcactgct
    26401 tttccgatca cacctgagct tgtgatatct cccattttcg taaaattctt ttccccgact
    26461 agtccaaaga cttgtacttc atcaaaagca ctagcaatct cagatggcag ctcgccaaaa
    26521 gacttgatga cttcattttc tttgtaacca tccagttcga ctacttgttt attcactggt
    26581 tgagcaccac caggagacat caagacccac ttttccatgt ttttcggttg agataagaaa
    26641 gctgtgaatg cttcagcagc ctctttttgt ttctcgtcta gaccagaaga aatcgtcaat
    26701 ccagatactg tgccatagac agcttgatct ttttgtgttg ggatagcaaa accgatattt
    26761 tctgagttcc ccgcttcata aacagaaggc aaaatgtaag ttgaatagat tgccattggt
    26821 gctgttccat tcatgaaagc atctttgatt tctgtcacat cattagagcc tggcatcgtg
    26881 tattgggata agttttgata aaacgacaat gcttctctca tcttttccgt atcgatcgtt
    26941 acctcgcctt catcatccaa aacatttgct ccatttgaca aagcaaattg agagaacgct
    27001 tgctcactca ttgttccttc agcagtaggc atagcaattc catattgttt attctcttta
    27061 tctgtgaaat gttgagcaat ttttaaaata tcatcccaat tttctggttc tgaaaaacca
    27121 gcttctgata atttttcttt gtcataccaa ataccttgaa cccagccgct aattggtgca
    27181 gcaatatagc tttttccatc ttctgaacga acaaggtttt tggctccgtc ataataatta
    27241 tcttctccag cttcctcgat aacgttttgg actgccgtct gatcaatcag ttcatcttta
    27301 tccatcactt tagcaaaatc ctgactgact tccattactt ctggtaattt accggaacga
    27361 gctaacgtaa cgatttttgt attgaacgct gattcttcta ccggcacttg cttgattttg
    27421 atgtctggat tttctttttc aaaatcagca accagttcat tgatcacact taatcgttcc
    27481 tgttcgacag aagagtgcat gaattgaatc gttgtctgtt gtgattcgtt agagctttga
    27541 ctttcaccac aagcagtaag aagaaatcca gtagctagca gacaggtagt aacaataatt
    27601 tttttcattt ttttccacct cttattttat tattttcagc cacatatatt catacggctg
    27661 gaatatcaac tttcgttctt gactgcaatg gataatttgt ccagaaaaca ggtctaggta
    27721 atctcctttt ggtaaaagat aacctgctga ttgattggaa aaattatgga taactaagac
    27781 tttctcttcc ttcgcttcgc gaataaaact gataaaagaa tctgctgagt ccagtaagta
    27841 aatcaaacta ttcggatgga aggctttttc tttttttcga atagatagca tctgggtcaa
    27901 ttcaccatat actgctgttc ttaatggatc tgtctgcaac tctttctcaa tatctgctaa
    27961 caaaaatttt tgccgattga tttttcgatt ctctcctgtt ttttctacgc ctttgtaatc
    28021 atttcgactt cccagcacac tttgaatata aattgccggc acgcctggta acgttaccaa
    28081 aaatgaatga gcagctaaaa agcgtttgct tctccattcc gtcgtatctg ttggagaact
    28141 taacgcatct agatacgtag cgtttatctc atatggactg gtcgttccat ctgggttttt
    28201 cttgtagttt actcgggtac cctctttctt cagctgttcc actaattcaa ggatcgtttt
    28261 ttccggcaga atacctcgta ccggattcaa tccgataccg tcatgggagg ctaaaaaatt
    28321 aaaaaaagtt gtttttttcg atgcgaaatc gatggttttt aaccattctt tcaacttact
    28381 tgtattaccc gaacgaatcg cttgaagggt caaaggtggt agcgaaaact gatagaccat
    28441 ctgtgcttca ttctcgccat tcccaaaata actgatatta tctttgtgtg gcacatttgt
    28501 ctcagtgatc aataccgttc ctggagctgc ttcatcagcg attgcacgga ataattggat
    28561 gattttatgc gtttctggca ggtgtataca agaagttcct agttctttcc ataaaaatcc
    28621 cacagcgtcc aagcgaataa atgaagcgcc tttttctaag taaaacaaaa gaatatcgat
    28681 cattttcaat aaaacttctg gatttttaaa attcaagtct acttgatctt cgctaaaagt
    28741 cgtccatacg tgtttttctt tcccgttttt gtcaatgaac gttgtaagta acgggctggt
    28801 ccttggtcta gtcacgctgc ttgtatctgt atccagtggc aaatcaataa aaaaatcttt
    28861 gtacgtatca ttcaagttta aatattcctg aaaccaagta ctttcggcag acatatgatt
    28921 gcagacaaag tcgaacatta accgtgtttc gcttctcatt tcttctatat cttcccatgt
    28981 gcccaaacgg tcgttgattg cctgataatt tacaacggag aaaccatcat cagatgtata
    29041 tgggaaaaat ggcaagaaat gtacaactct aaatgtatct gccaaatact tttgataaaa
    29101 tttcttaaat gtacgcaaag ttggttgctg ctcttcaaaa aattgatccc cataagtaat
    29161 caagaaaata tcttcttcgt cccaatgcag tttgcctgcc tggcctttct tctgctgata
    29221 aacactgact ttttgttcaa tcatagaggc ggttttctca gacaaatctg agtcgcgata
    29281 aatcgcttgt aaaagttttg tatacatcgg attccccttt cttttttggg agcgttccca
    29341 acgatttgat catattatgg aaaacgctta ctgtcaatag cattttttta aataattttg
    29401 atatacacaa aaatacaaat aagaaagctt atgtatcaat aattaatagc ttcttaaaaa
    29461 aatatggtct ctcatgatca tgcaagctat tcctaaattt tcaaaatcat agacaaaaaa
    29521 gaatgtctat tgtactatgg aattattcta aaacgctccc aaaaaggagg tgcatataaa
    29581 attgaaacct actatttatg acattgcgaa aaaagctaat gtctcaaaat caactgtctc
    29641 cagagtatta aataaccagc caaatatctc tgataaagca aaagcaagtg tacttcaagc
    29701 aatcaaagaa ttgaattatg aaccgagcaa aattgcaaga ggactgtcag gaaatggttt
    29761 tgacgcgatt ctagcattat ctcatcgtac ttctcatacg acctatggca atcctttttt
    29821 ttcagatgtt attcaatcca tttctgccgt ttctgaacaa aataacttcg acttgattct
    29881 acaaacggca aaaacaccag aagaagagct agaaaaatgc gcgaaaaaga tccaagaaaa
    29941 aatgatccga ggaattatta ttttaagctc tacgatggat gaaacacttc tttttgaatt
    30001 ggatcaatac aatattccta ttgtggtaat cggaaaagtc ggaaacgact acacgaacat
    30061 ctactcagtg gacacggata atttccagga tagttacgat ttggtggaaa cactgatcaa
    30121 gaatggacac cgtcatatcg gctgtctcca tgctccgaca gaagtttatg tatccaatga
    30181 tcgtctcaat ggttaccgtc aatgcttatt tgatcaccac ctagatatcc gtaatgaatg
    30241 gattatagac ggcggctttc gcttagaaga tagcatggca gcaatgaatg atttaatgaa
    30301 gctacgagaa agaccaactg cgattttttc taccgatgca ttaaaatcat tgagtattta
    30361 caaagtggca gaacaatctg gaaagcagat acctgatgat ctttcgatcg caggattcag
    30421 tgatccctta ttgtcctccc tattttcgcc acagttgact cggatagaca ttccgacaaa
    30481 tcagttagga atcaaagcca cagaactttt attccaacta atccaccacc aataccctga
    30541 agaaaaaaga atcattcttt caaccgagat tactctcaca gattcgatcc aacaacaaga
    30601 aatgtagaac agaattataa aagaacagaa acactgagga taagtcagtt actctgttct
    30661 tttttcaatc actcatccct cgttaccccc tcccggtatt ttagcgaatt tttcttattt
    30721 tccatctgtt ttagatagct gtggataact ataatgtttg tgttaaacta ttttttccac
    30781 agggaagaaa aaactatttt taaccacgtt gtgtataagt ttttatagtt cgttttttta
    30841 aacacattta cccttatttc aatccatgtt atttggggat acttatctct aaaacataaa
    30901 tagaataaca gacatgtcta ctggttttgt tttattcgag gaattattca ataatagcta
    30961 gcaaaatatt aaaaataacc gctctatctt cccatcaatt tattttgcat tcaaaaaatg
    31021 atctatcttt acttttgttg attgacttct cttggaagat gatcgttccg ataccttaat
    31081 tggatacgaa ccgatgttgt taagatcctt tcccactttt cttctagtta gacaaattat
    31141 tttagacaaa ccaaaaaaga ggttgtgaca ataatttgtc acaacctctt ttttaaaa
//
LOCUS       DACDUU010000027        30860 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.027, whole genome shotgun sequence.
ACCESSION   DACDUU010000027 DACDUU010000000
VERSION     DACDUU010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.027"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..888
                     /locus_tag="IWL30_001291"
     CDS             <1..888
                     /locus_tag="IWL30_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAP9706068.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     RVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
     gene            complement(938..1240)
                     /locus_tag="IWL30_001292"
     CDS             complement(938..1240)
                     /locus_tag="IWL30_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9706069.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(1265..3358)
                     /locus_tag="IWL30_001293"
     CDS             complement(1265..3358)
                     /locus_tag="IWL30_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9706070.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(3847..4611)
                     /locus_tag="IWL30_001294"
     CDS             complement(3847..4611)
                     /locus_tag="IWL30_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAP9706071.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(4722..5591)
                     /gene="mdcB"
                     /locus_tag="IWL30_001295"
     CDS             complement(4722..5591)
                     /gene="mdcB"
                     /locus_tag="IWL30_001295"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAP9706072.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(5616..6692)
                     /locus_tag="IWL30_001296"
     CDS             complement(5616..6692)
                     /locus_tag="IWL30_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAP9706073.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(6716..7309)
                     /locus_tag="IWL30_001297"
     CDS             complement(6716..7309)
                     /locus_tag="IWL30_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAP9706074.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(7313..8989)
                     /gene="mdcD"
                     /locus_tag="IWL30_001298"
     CDS             complement(7313..8989)
                     /gene="mdcD"
                     /locus_tag="IWL30_001298"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAP9706075.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(8979..9278)
                     /locus_tag="IWL30_001299"
     CDS             complement(8979..9278)
                     /locus_tag="IWL30_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAP9706076.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(9380..11026)
                     /gene="mdcA"
                     /locus_tag="IWL30_001300"
     CDS             complement(9380..11026)
                     /gene="mdcA"
                     /locus_tag="IWL30_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAP9706077.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(11033..11944)
                     /locus_tag="IWL30_001301"
     CDS             complement(11033..11944)
                     /locus_tag="IWL30_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP9706078.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            12064..12921
                     /locus_tag="IWL30_001302"
     CDS             12064..12921
                     /locus_tag="IWL30_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9706079.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(13108..13470)
                     /locus_tag="IWL30_001303"
     CDS             complement(13108..13470)
                     /locus_tag="IWL30_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706080.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            13816..15255
                     /locus_tag="IWL30_001304"
     CDS             13816..15255
                     /locus_tag="IWL30_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9706081.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(15262..17814)
                     /locus_tag="IWL30_001305"
     CDS             complement(15262..17814)
                     /locus_tag="IWL30_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAP9706082.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(17928..19259)
                     /locus_tag="IWL30_001306"
     CDS             complement(17928..19259)
                     /locus_tag="IWL30_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9706083.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(19225..20703)
                     /locus_tag="IWL30_001307"
     CDS             complement(19225..20703)
                     /locus_tag="IWL30_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9706084.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(21008..22843)
                     /gene="tdc"
                     /locus_tag="IWL30_001308"
     CDS             complement(21008..22843)
                     /gene="tdc"
                     /locus_tag="IWL30_001308"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP9706085.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            23313..24740
                     /locus_tag="IWL30_001309"
     CDS             23313..24740
                     /locus_tag="IWL30_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9706086.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(24832..25197)
                     /locus_tag="IWL30_001310"
     CDS             complement(24832..25197)
                     /locus_tag="IWL30_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP9706087.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(25194..25559)
                     /locus_tag="IWL30_001311"
     CDS             complement(25194..25559)
                     /locus_tag="IWL30_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP9706088.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(25751..25999)
                     /locus_tag="IWL30_001312"
     CDS             complement(25751..25999)
                     /locus_tag="IWL30_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706089.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            25998..26126
                     /locus_tag="IWL30_001313"
     CDS             25998..26126
                     /locus_tag="IWL30_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAP9706090.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            26183..27877
                     /locus_tag="IWL30_001314"
     CDS             26183..27877
                     /locus_tag="IWL30_001314"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAP9706091.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            27944..28150
                     /locus_tag="IWL30_001315"
     CDS             27944..28150
                     /locus_tag="IWL30_001315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706092.1"
                     /translation="MFLIWLDLLPQMQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFL
                     TWLDLLPQMQAFEHRLPRGYEKGV"
     gene            28528..29661
                     /locus_tag="IWL30_001316"
     CDS             28528..29661
                     /locus_tag="IWL30_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP9706093.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            29692..30012
                     /locus_tag="IWL30_001317"
     CDS             29692..30012
                     /locus_tag="IWL30_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706094.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(30041..30505)
                     /locus_tag="IWL30_001318"
     CDS             complement(30041..30505)
                     /locus_tag="IWL30_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9706095.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(30637..>30860)
                     /locus_tag="IWL30_001319"
     CDS             complement(30637..>30860)
                     /locus_tag="IWL30_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9706096.1"
                     /translation="VSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAA
                     VNLVLSILIYLPFVKVATIQERRKEALDA"
BASE COUNT         9423 a         6332 c         5320 g         9785 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt acagggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt caacttttgg
      901 ggttcagttc agtttttacc ctcttttctt tttgtattta gataactccc ttatcttgca
      961 gaactggggt gattttttct tcaatttcat tttttgaaac aatatttttc aaatagatca
     1021 tcgccgtttt aggatcaact gcgaactttt cgaactgcga acgaaaattt tctgctgtga
     1081 tgatcacatc tggtttcatg gaaactgcgg aagaaatatc acaatggtcc agtttggctt
     1141 ctactcctaa tttgttcaaa acatcctcag cagacatctg tgctgcaaaa ctacttccta
     1201 aacctgcacc gcatacgaat aaaatgttca atttgttcat ctttgttctc ccccgtatca
     1261 tttattattt ctcaactact gaaaaaagca gttgtttgaa atcttcgata tttgtgcatt
     1321 caatcaatcg atccaacttc tcttcgttgt tgatcaattc aattagttct ttcatgatgt
     1381 tcagatgcga gtaggagtct actgctgcca aacaaaaaat aatctttact ggatccattt
     1441 caggattacc gaaatcgatt ggttgctcaa tcgtagctac gctgactcct aatttattga
     1501 ccccatcttc tggtcgggca tgggctagtg ccatgtgttt gccaatcacg atatacggtc
     1561 cgtaatcatt cactgcacga atcatcgctt cgacatagcg ctcttcgaca atgttttctt
     1621 taaccaatgg tctagcagct agttcaatcg attcctccca agtagtggct ttttctttga
     1681 tcatgatatt gctgtccgtc aaaatatctt ttaacatagg ctggatctcc cttttgttga
     1741 tttctaaatt gtttgtttcg aataattttt ctaacttgcc atagatgatt cgatctactc
     1801 ggccaccact ttctttcatg acatctatta tttgagtgaa taactccgtc gattcatgac
     1861 tgttaggctc taaccgctgt agttcttcat ttcttttcaa aaagtcttct ataaccaaga
     1921 tattcgtctc agttaaaatc ggatcgacaa ctaaaagcgg cttatttcga taattcaatt
     1981 gaaaggtgct gaaaaccaga ttgacatcca atttatctac taattcgatt tcttttgaac
     2041 ttaaaactgc agtaatctcg atctgaggaa acttttcttt cagattctct gctaacagat
     2101 ttccagtagc catcccatgg ttacagacaa cgactgattt ataaacataa ttcaagtctc
     2161 gcttgattac actggcgatc gtcgaaaagt ggatgacaag aaaagctatc tcgtcctcca
     2221 gtatcttgcc accaattact tcttcgatcg taggaacaaa atgagtgact gcgcggtaga
     2281 ttgggaaata atttgttcgg atgttttctt tcaatggatt catgacttgg atatgattct
     2341 tgattcttac aatcagtgca gccatgtgct tatagagatt ttcgaataaa gcttcttcct
     2401 ttagatggaa aggtacgtgt gtctcgttct ctacaaattg gatcaattgg atcgttagta
     2461 attgagcttg tacccactcg attgaattgg agatatctct agcattgaac gtctcaatag
     2521 tgaaacgaag ataatttctt tctacttgcg ggacattgtc caattgaaat tcctcgatca
     2581 tgtggtcaat aaatgcagaa aagctactgt cttttatttc tgtatttttc caattaatgg
     2641 cagagataaa ttcatgtttc ctgattcgct ggatccagat caatgtaaaa agcaggattt
     2701 gttttttata aatatcatct tcttgtttca acaacttatt ggaatagatc tcctgtattt
     2761 ttttgatttc caccaaagga aaataatgga gaaagatttt ttgagccggt gttaattgtg
     2821 catcttgatt cataaagaag tcggtcattc ccagattctg attgataaaa tcatagatca
     2881 tcgtacggat agatcgttct gaacctttat aaaccagtcc ttgctttcca taactgacga
     2941 tctctatccc ataactgacc agtgttgctc gtaaccgtct catatcttca tctaatgtac
     3001 ttttagagat ttgaaattct tcttcttttt tgtttagatg gatcgggatt cggctgtaag
     3061 caacggctaa aactagatca aaaatacgtt cgcgccgatt caaaaagccg tctttcacat
     3121 cttttatttc agtttctaat cgagcttgct tttccttttc cagcttcaac aaaaaacctt
     3181 tgttgcgaat tgtacggatc tccggaagct ttttactgct caaccagata ttcgcttcaa
     3241 gaacttcctg acggatcgtc cgctgactga cattgaactc acttgctaga tgttttgctt
     3301 ttactggata ttgggctgtc atgattctgt gaagtatttc aattgttctt gttttcatag
     3361 aaatgatctc cttctgattt caagtatatg gttccgctta cataataaaa agtagatatt
     3421 tctgccactt gaagtggaaa gaaagtaagc aaagcaaaca aaaaataagc catatgcttt
     3481 ttcatctcct caacgaaaaa gacatacgcc tattattaca aaaattttgc ccatctatac
     3541 aaaaaagagg ttgtgaaaaa gctgtcctgc atcaagtaat aagaacaact aaactaaaat
     3601 agtgcttcct atttttcgtt tggttgttct tattatcgca gatgcaagct tttaaacacc
     3661 atttagtgga gaggttgtga cagagatgtt cagccttgag cgataagaag cgattttgaa
     3721 aaatcgcttc ccatatttta caaaatcgtg ccttattgcc gataggctat ctctggcaca
     3781 ccgtttattc agaaaaagga gctgcgacaa ggcttttgtc acaactccgt tcataaatat
     3841 ttcctgtcaa tccagcataa tcggctgaat aaaatcggct tgccatcttt tttctttctc
     3901 agaaaaatca aaataccata tctgagaaaa atactgtcgt tgtgtcttac ttcctgagat
     3961 caatcgccca tcttttcgat cgactgaata atcacagcat ctgaaatcaa ttcggatggc
     4021 aaaactattt tcgtggatct gtcgataatt tcctacttcc tgaatagtta tttttttagt
     4081 atgatttctt acaccgtctg ctttgttttt ttgcagaact ttctgatgtt cggcaaacag
     4141 cttgtctgta taacagcttc tggctttttc aggagtttct tcttcccaag cggattggat
     4201 cttcatgaat atcttttgga tttccagaaa ctgtgctttc ttttctttag ccgttcctga
     4261 catgcgggac aatatctccg ctttcagcct tttcttttgt cttttattct tgacgataga
     4321 aaaagcagaa acccctccta ctgacaaaac agctaatgca agaaatcccg tttgctcata
     4381 actagaagga cctgtacctg gaccataata cccataagaa tctctgccgt aagaacgact
     4441 gccaaaataa ctgcccccat atgaacgacc accaaatgaa cctctgcccg aacttcgacc
     4501 gctagatgta cctccaccat gaccacccgc gacagcgaat ccttgggtcg gcagagcaaa
     4561 caaacatagg cccactaaca aaatgattgc taaataattg atttttttca ttgtcttcct
     4621 cgttatcttt tattacaaaa aactattttt atgatagcct tgctaaaagg aagagtcaat
     4681 aaccagatgc ataaactgtt tttttagcaa ttttgtctta tttagtcatt gagtaacatc
     4741 ggacaaatgt atgtgtcggc agtccataat tcctctatct tcagcatgaa caagcttgca
     4801 gacaagagat ctgcgcttcc tccaggagag atttggtttt cctgacaaaa gtgcaacagt
     4861 gctgtaaatg gacgatttgg taaagcactt tgactagcag catgagcaag tttttgcatt
     4921 tccactaacg ttttctgatc actgcgatgc aatacgcatg tgtcatctag cgaactcatc
     4981 aaaactaaca acatatgcat ttgtttctgt acatatgaat cattatctag ctccttagct
     5041 gcttgaattg caagcctgat atgcggatac cctaatatcg cttcttcata tgcaccattt
     5101 acgccatatt tttgcttcac cctatgacca tgagtggttt ttttatgacg atattttgta
     5161 tcaggaaaag cagcaagctt gctgacatca tcaagtattt tcgataaaga aaaaggctgt
     5221 tccatactga ttttttgtgc aatgacactg atcaatagcc ccattgccca aatcgctcct
     5281 ttatgggtat tgatgccatt tgtagcaatc agcattgcct tttccgcttt tcgcccaatc
     5341 tcagcaattc tttctcttaa ctcctgatca atagcatgat gccatgctat ttgagccatt
     5401 tcttcaaaaa aaggagttag cgtcttagca gacttaagaa ataaagcata gtccatatcg
     5461 gaatgtgcac ccgcatccag tgcatccact aatccaggtt ttggacttaa tgttacttct
     5521 gcgatcaatg cttcctctac caacgcagcg agttgctgag tcactgcttt ttttcgttcg
     5581 tcatttatca tgacactcac tccttcttcc tactattact tgctgaaacc ttttttgata
     5641 gctacccaaa tgatatgaag taaccagctt aaagcaaatg cacaacttgc cgtcattaga
     5701 taagctacaa gaatcatgac tcctgaaggt aactgcagcc aactatcttt aaacaataat
     5761 aacagtactt ggaaaacaaa tacattggct aaatacgtac gataagcata aacagacaac
     5821 catttgaaca atggcaattt tggcgaatcg tttacaatca ttttggaagc gcctatgaag
     5881 acagcaaaga tgatgactaa actgtataaa ctttggatcg tattcaagta aggtgcattc
     5941 gcaaagctca agtctcctgg atgtgcgagc aatgaacgga cagaaaagaa cgcgataact
     6001 aaggcaacag gtaagaaaag ataacgtact ctgtccaaat attgatagac tttttcgtga
     6061 taaacaaatg cggctacccc ataaatcatg aaactagcaa caaaccgatc taataagtac
     6121 cagctgtcat gataaggccc agtcaatact tgtgtttgat agaacagata ccatgcgaca
     6181 tataaaatcg ttgaaccaat caatactggc catacatatt ttttattagc aaatactttg
     6241 taaccgagcc aaacaaaaaa cggcatgaac aattggatct gcagcatcat aacggtatac
     6301 cataaatgag gcgctccatc acctgtgaca tatttcagta aaaagcttgc agtatccgta
     6361 tacggagttt gttgttggac tgctggaaat agccaaagat aagccgtcgt ccatagaaga
     6421 tacggaacga ctagttcaaa aaacttttct ttcaaaaatt cccaataaga caaatgctga
     6481 cttgttttta ccatattata aacaattgca aaaataaaca ttggtgctgt gtatttaacg
     6541 atgacgtaaa aggtaccaag aaaagctgta tttgttgcgc tgtggctgtt tgccaaagca
     6601 aagctcaata tcgtttgcat cataacagcc gttacagctg atactttctg gaagtcactt
     6661 acgttgattt tgattttttt gttttcaaac atctctttct ctctcttttt ctttattacc
     6721 aaagttcttt ggttaattta ggtccttcga ctgttttcaa tagaattttt ttctctggct
     6781 ctctcaaata ctcttttagt gaaaaaccgc cttgccgagt cactacttgg gtatcgaatt
     6841 gctcaaaaaa agcaggatgc gattgaatta cctgaattgg caattcaatc ggtgaatttg
     6901 cgtacagcag caaatcaaaa tcactggttt cttttactgc cggaatccca gtcgataatt
     6961 caaatcctaa gctgccgccg attccccatt tacatcctga taatatgttt ctagcagcat
     7021 gaaattgctg ataaacagga taatccatga ttttctcttg tctatacgat tcttgttcga
     7081 tgatgtccca cggatgaatg acttgtttga cggcatgttt tgagatttct gttgaccatc
     7141 tctgattgcg ggcaggtcca cgtaaaccaa tcggtagtat ttctgcgtga tccgtcgacg
     7201 cacgacgaac tgttccgaac atcttttcag ttagaacgac ccattcaggc aaggtgaacc
     7261 cttctatttc atgtttatca aaaaggacaa tatcatgtgc ttgaatcatt ttttatgcat
     7321 cccattcttc gtccattttg gcacggactt tatttgtttc gacacgtccg aatgttttgg
     7381 ctgtttcgtt tgtgtatcga tttgtcagca tcgtgtcatt tgtattgcgg acatcttgaa
     7441 tggcttcatt gattgcttcg atgactttgc tgacattttg ttctgaagca gcttggtcgg
     7501 tgatatcttc taggaaatga tagatcgctc ctagttttgt ataattattg atatcgtaag
     7561 cgatagctgg aactttttca gcagctgctt cgatttcagc aaccgaacgt tttgtcacac
     7621 gtgcagcact ttctttagac atcgcttgga cggtaatttc tttattgttt aaagcaatca
     7681 agcggcttga ttgtaatcca tgtgctaaaa atgcacctga aatagcattt cctgcaatca
     7741 atccaataac tggatgtcca gcttgacgga gttttgcata agcagcagca ctattagcca
     7801 aagccacatg gataccgatc aattcttcgt tatagccgta tgcttggctt ggtacatcga
     7861 tcacaacgat gattggccgt ttcacttttt tgtctttatc ttcttcgtaa acgtgatcca
     7921 aaatttgtcc catgcggaaa ccttcaacta aaccaacttc accgtttcgt acacggtaca
     7981 tcgggttttt aggatcaggt acgattgatg tcacgatggc ttcttctatg aatttacttg
     8041 atttaccata atacacagta ctaatagaag aagttggatt tttcatgcca gttagtgccg
     8101 caaaccaatc gtaaccaaca cttgctgcag ctggttcatt tccttctgct acttctggta
     8161 agtcaattga ttttgcttga tggttagcaa ttgcttcatt gtaatcgtca atggccattg
     8221 gttttgaaag atctaaagaa tcaagtaatg ataaataaaa tgctgagctt tcactgcgat
     8281 gcgtgtcttt tcgttcgtcc attgctgcga tgacagcagc tttgattgct tctacactat
     8341 cagtcactaa ttcatcaatg atttttgatt ctacccgttg acgagatcct aatgtattcc
     8401 aaatcaagtc tttgtcacta gaatcaaatt ctcgaacgcc tgcttcttgt tcaataactt
     8461 ctggtccatt caaaccgact ctcgcttttt ttgttgcgat taaataactc attaatgctg
     8521 aagtaatcga cataccgcca aatgatccta cacgtcctgg aatcaaaccg atgactggta
     8581 catattgttt tagagcaatg attacatttc cgatttctga aatagataac agtccgtagt
     8641 ttgcttcttg caaacgaaca ccacctgtat ctagtacaat gactggaaag atctcatttc
     8701 cttttttatt ttcttctaac acatgttcaa gtgcagcaac gatttttgca ccggaaactt
     8761 caccgatacc gccaccttgg aattttcctt ccatagcgat tactactgct tgtttgccat
     8821 tgatcgtgcc acggctgaca acgatcccgt catcactttc agggacgatt ccttgtgctg
     8881 gtaaacttgg cgcgatgatt tgatcgaatg gatcaatcaa ttcacgtcca ttgtcaagta
     8941 gagcgttcac tcgttctctg gcgtttaatt ctacaaaact atttttcatc tgaaagttcc
     9001 tccattgctt gcgtcaatcg taggtacaca actcctggtg tagcaccgaa gtcattgatc
     9061 acgatatctg ctgaaattgg ataacgatca aaaaatctcg ttaatacatt gttccatact
     9121 tcgccgaagc catcgcttcc tgtacaaatt tttacagtag attctgtttt ttctgttgga
     9181 aacatcaaga tttccaaatc tcctgaaccg accactccta catggatttt cttattgatc
     9241 ggctgtttcg ttggatattt ataagctaat tgttccatgg atctctcccc tttttgtaaa
     9301 acagagcata cacgttctgc ttattcgtct ttcttcttcc atctgggtaa atggcgacaa
     9361 aaggtttttc tcgccaattt tatgaccaag ttctgaattt cattggtggt tcatacaggc
     9421 catctgacca ttcgatcaaa tcatcgatgg tttgtgcagc taataatgaa cgtttcgctt
     9481 cactacgttt gatgcctaga tcttctggta atttcacgat gccggctgca cgcaagctat
     9541 ctagtgattc tttcgtgcta gtcattccaa gaggtgtaac accgccaatt gctgcaattg
     9601 ctgcttgtcg ttcttctttt gtttttgctt tgtacaaata agcaattcct tcttctgtca
     9661 cgatatgtgt agtatcttcg ctataaatca tcactggtgc attggctaag cctgcttgtt
     9721 tttgaacagc tagtgcatct aattcttcga caaagactgg ttttttattt gaaccatatg
     9781 tttcaaccat ttgaacaact aatttttggc ctttccctaa tggatcatca ttgtcgcgaa
     9841 gtgattgcca tgctggtgta gaatgacgac gaccgccagg attatgtccc atgttaggcg
     9901 caccgccaaa accagataaa cgtccttttg ttacggtaga agagtttcct tcgtaatcta
     9961 attgcaaggt tgaaccgata aacatatcga tggcatattg tcctgcagct tgtgataatg
    10021 ttcggttact gcgcatcgtg ccgtctttac cgacaaaaaa gacatctggg cgagcagcaa
    10081 tatatttttc cataccaact tcgccaccaa aactatggat gctttcaacc caacctgatt
    10141 cgatggctgg aatcatcgtt ggatgtgggt tcaataccca gttcttcgcg attttccctt
    10201 ttaaccctag tgattcacca taagttggca gtaacaattc gatagctgct gtattgaatc
    10261 caataccgtg attcaaagat tgtacgccgt gttctgcgta gattcctttg attgccatca
    10321 ttgccattag aatttgcaat tctgtgatgt tttgaggatc tcgtgtgaat aaagcttcta
    10381 attgataagg tttatctgct ttcacgacaa cgtctaccca gtcaccaggg atatctacac
    10441 gtggtaaaga atcgacaact tcgttaactt gaacgatcac gataccatct ttgaaagctg
    10501 cagcttcgat gattgttggt gtttcttccg tattgaaacc tgtgtacaag ttgccgtctt
    10561 tatctgcttt atctgcagct actaaaacaa catttggaat caaatcgatg aataagcgtc
    10621 cataaagttc caaataagta tggatatcac ctaatttcat tgtgccgtct tcaatcattt
    10681 gggcgacacg caagctttgt gcaccagcat aagagaaatc gatcttgctt gcgattcctt
    10741 tttcaaaaat attcaaatgt tctggtcgtg agatactaga catgatcaaa tgcagatcat
    10801 tcactttttc tgcatctact ttttcaagac tttctgaaag aaaacttgct tgtttttgat
    10861 tgtcgccttc taatacaact ttgtcgccgg gagcaatcag tttttccatt acttctacga
    10921 ttttttcagt ttctacatac ttgccattga gcaagttgct gattttttcc aatcgctgcg
    10981 cttttttttc gcgtttagaa gcccagttgc ggtttggttt ttccataatt agtcatctcc
    11041 atttttgata tagaaacgtg gcgtcatcaa taccatgttg gtctagattg atcgtacgga
    11101 tccctttatc tacaccaaat tttgtatgaa tcagctttgt caaagtattg ccggggggaa
    11161 attcaatcgt cagatccatt cctaattctt ttgccaagtc catcatttgg ttccactgca
    11221 cagggaaagc taggttttcc aacaaatctt tacgaatctc ttctgcgtcc tgagtggctc
    11281 ttccatccgt attttttata tacaaacatc tagcatcaga tagatggaca gactgcatat
    11341 acggcttgaa tttcttgacc gtatcattca tcaagagaca gtgagatggc acagggactt
    11401 tcagcagctt cgctgtttgc gcttggtgcc gtttggcttc tgccaacgta acggataaac
    11461 cttcgatact cccagataac gtatgttgca tcggacaatt ttcatttgac agataaactg
    11521 gatggtcagg atcaaatgtt tttgctacaa tttcctcagc ttcgcttctc gtcacaccta
    11581 ctactacgcc cattgcataa ccgcttggat aagcttcttt cattaagact gcgcggtgat
    11641 agactagttc cgctgcattc tcaaatctca aagatcgaca agcaacagct gcacaaaaag
    11701 caccaatcga atgtcctgct gcaatatcag gctgaatacc gtttttttcc atttcatcta
    11761 aataaaatac tgcttttgtc agtaatgcta gctggataaa aaccgtatct tgatagtttt
    11821 cttctttatc aactaaccga taccccgtca cattttctaa acgttccatg tacatttcag
    11881 ggatatccgc taacatccct aacttctgac tgccttgtcc aggaaataag aaacctgtac
    11941 tcatgagcct cgcctcctca gcacttactt tttccactgc tatcttagtc aatttctgct
    12001 ataataaaaa atgccaaaaa aagaaaaaac cataacaaaa aggtatggat gggatggata
    12061 attatgaaat tgcttcatct gcagtatttt aaaaaagtat tcgaaacaaa aaatgttacc
    12121 caagcagcca aagaattatt tatttctcaa cctgccttga gccgtgcgat caaacatttg
    12181 gaaaacgaat taggtgtccc tttgttttac catagcggca gaaacattga gacgaccgtt
    12241 tatgcagaag aattttatcc atatgcagtc aagacattag acacattaga agaaggaatc
    12301 aaattattgt cttcggtcaa tgagaagatc gttacttccg tcatattgta tttagaggta
    12361 gcctctgttt ctattccgaa cttggtccga atttttatgg aaaagcatcc tgatatccaa
    12421 ttaacgatca tgcaacatca tttaccagat gatattaccg atcctgtact gtacatcact
    12481 tcggagatga agcctggcat gaccaatatt cctatcatga aagaacctgt ttatgtcgcc
    12541 attcctaaga aacatcctct tgctaaaaaa gaacatctgc aattagaaga tatccaacgc
    12601 acccccatct tgatgctgtc aaagagcaac gccttccgtc atacactgga tgcagcactt
    12661 gaagcgaaaa gtatcgaatt gacgatcagt tcgacgacag atgatcctgc gacattacgt
    12721 tctattgtca agcaaggatt agctgttagc tttttcccaa aagtttcttg gtcttacgat
    12781 aaaaatgatc ctttcgtctt gcgggaaatc aaagatttgc ccttgacacg aacaatttat
    12841 ttatcttcta gcttcacaga agatcatcct ttgatcaaaa tgattgcaaa aacgatgcgg
    12901 gaattttatt taggaaaata aaaaaaaaag agctgggaca aaattcttgt cgcagctctt
    12961 ttttcgaaca aacggtgttc cagaagtaat ttcttagaaa ataagccgaa atttcccaaa
    13021 aatttgagaa gcaattttcg taaattcctt cttatttctt gaagctaaac atttctgtca
    13081 caacctcttc tctattgtcc tagacattca ttgcgttgat ttaaaacttc catcaatcaa
    13141 atctgctagt tcatctagct tttctatttt acgtagatca atagagatcc aatgttcttt
    13201 attcatgtga taggctggaa aatacccctc ttttcctcgc aaaattccgc caagttcagg
    13261 atcgaccttc aaatcaagaa tattgatgat cctatggtca cttgcatcat catagatttt
    13321 ttcttctgga acagacatca ggaccccaaa ccattttttg cttttttgat gtctgaatac
    13381 gcaataatca ggaaattttt taaaaatata ttctggagac aaattgtatg tttcattgat
    13441 gtgatcgatt agttcttttt tcgttagcat ctgcttccat ctctttctta ttgattcgct
    13501 ttattcaaag ataccacgta agaatagaaa aatgaataca agtagttgct aatcaaatac
    13561 ttcgatattt ttctttttta tcaactctta aatgttcaat ccgcctgtgt tggtcttgct
    13621 accatctact attaaaatgt ttattattgt ttgcccacct attttttgat tttagtggta
    13681 tcatttttca agttcatgat gaaattctta tcatcagtaa atgctccaga aaaataaaca
    13741 atgattgaaa aatcagttga aacttgacca ccgtttattt tctccgtatt cttagaatag
    13801 aagggagtct gattgatgta ttcactaatg aaaaaaatca ttaccgaaaa agatattcga
    13861 cgtcatgttt ctttagtaga acaattgctg aatcatacaa agataaccgt aaatgaatta
    13921 gctgaaatca tagggacaac cgaacgaacg attttttctg accttcaatc tattcgctct
    13981 cagctgcccg aaggctggga catcatcagc gatcaagcag gaatcagttt gcaaaaccag
    14041 caaaatttat taacaaatga cctttgggag atttttttca aacaatcagt cagtgtcgaa
    14101 ctgctgaaaa atctgctttt tacaaaaaaa gttgctgtcc ctgattttct agccgaccat
    14161 gggctttctt atgggacatt gaaaagacat gtgactaaaa tcaatcagcg tttagcaagt
    14221 tacgacttgc agatcgattt gacgaaatat acagcttgta ttcttggaaa agagcgtgcg
    14281 attcgtactt tttatcatcg attgctgatt cctttcactc ataacaacta ttttttcgag
    14341 gattattcga tccatgaatc ccattatttt caatttttaa gaaacctcag tcaaactgaa
    14401 ctagcggttg aaacagaaga gattttcgga acatgttggt tctttatcaa tacgatccgt
    14461 ataaaagcga attgccgatt agattcttct atccatacca acagcacact ctcctccctg
    14521 tatgactctg cattgaaaaa attgtattta acggaaggta tttatcttaa agacactgag
    14581 ctttcttttg cttctttctg ctttctggaa agttggaatt acaacaataa ctatggacaa
    14641 gaaaccgctc gctgcctgca tcattctcca tttttagagg tcctcgaaac gtttgtagag
    14701 gaactagctt cagagctgtc tcttgatcaa ctaaaaaaaa catctctgac tgataacttg
    14761 gctctcctca tattaaaata tcatgaatcg ccgatattga ttgaagcatt ggaccgtcaa
    14821 taccatcgtt ttttagaaac atatgatcag caatatgcac acttgtatca acagaaagag
    14881 aatttactga acgatctaaa aaaagaaata gaaatcaatg aaccaaatta ctttctccaa
    14941 ctcttgtctc tactgatcca acaaacgatt ttttctatca aaccgaattt gttcaatgtc
    15001 tattttattt tccaaggaga accttcatgg aaggcgttct tgcagcagga attaaaagat
    15061 tacttaggaa aacgtgtctg cttcttacct atcgaactaa ccgatctttc cactatttct
    15121 tttgaaaaaa cagatatctt gatttccaat ttccctctag attctctaga tatccctatt
    15181 gtatacattt cttctattcc aacgaaaaat gagctcaatc gtttgactga actcactttc
    15241 aaaacttttt tatagtaacg gctagcgact gtattctcta gctgtttttt cttttttgcg
    15301 aaacagtttg ttcatgatcc ctttttctat ttctacaact aagaatagaa gaatcacatt
    15361 tgctccaatc aataactgtt gtgtcatcgt taaacttact gtaccgatca gctgctggcc
    15421 gactggtaga aaaataacca tcccttgcaa taaagcaagg atacctaatg aaagaaaaag
    15481 cgctttgttt tgcaaaagac cttggttcaa tgacggcttg gacaactctc tgcaattgat
    15541 catgtaaaca gcttgagcta agacgatatt ttgcagaaga atggtttgct gtaagctttg
    15601 cccttcaaac tgcatagcca ataaataggc aggaaccatg atcagtaggg agacatagat
    15661 gatccgaaac aaatgataac cagataggat tccttgcttt ggatctcttg gtgggcgctt
    15721 catgatttct ggatctggcg actcgaaccc tagtgcataa gataaagtga ttgtcgttac
    15781 catattgacc cataaaatct gtactgggga caaaggtagc gggcgattca gcataagtgc
    15841 ccaaatcaag atcaatcctt gagctaatgc agttggcaag aagaaattga tggttttttg
    15901 caaattatcc aaaatccgcc ggccctcttt tacggctttt gcaatcgtat ggaaattatc
    15961 atctcctaaa accatatcag ctgcctgttt gcttacttca ctacctttga ttcccatagc
    16021 aattcctaca tcggatcttt tcaaagcagg tgcatcattt acgccgtctc cggtcattcc
    16081 gacgatttct ccatttttct gcaaagcttt aacaattcgc agtttatgtt ctggtgtcgt
    16141 tcgagcaaag acatcgacta ttggcacttg ctgaatcaac tcttcatcag acataagatc
    16201 caaatcgatc ccttctaaaa ctttggcagt atgttttaag ccaatttgtt ccgcaatcgc
    16261 ttttgctgtg tctttgtgat cccctgtaat cattttcact gagatccctg cttgttggca
    16321 ttcctttaca gctttgactg cactttcttt tggcggatcg atcaatccgg cgatccctgc
    16381 aaaagttaat tgggttaaag tctcatgcgt gagttcctgt ttcgaatcta cttttttata
    16441 agcaaatcct aaaacacgct gaccttttct cgcaaattct gcagctgtct gactccatgc
    16501 ttcttgttct gcagaagaaa gagttgattt agcaaataat acttctggtg cacctttgac
    16561 gtaaatcacc gctccttttt cctccgcatg catcgttgcc atatatttgt attcagaact
    16621 gaacggaatc tttgcttcta tttttttcaa aggtagattg gctgtttctg tgtgtcgaat
    16681 cagcgcaact tctgtcggat tgccacttaa attttctgtt ttctgttctt cttttgtttt
    16741 taattcctga cagtttttca taatggacaa cacgcaggct tcttcttttg cagctacatc
    16801 cacaactgtc atttcatttt ttgtcaatgt tcctgtctta tctgaacaga tcactgtcat
    16861 agaacctaat gtttctaccg aaggaagtcc tttgatgatc gccttttctt ttgccatttc
    16921 atgtactcca acggatagaa tcatggtcaa aacagctggc aatccttcgg gtaccattga
    16981 caccaccaaa gcaatcgtag ccgaaagaag gatatgccaa tccataccat atcgaaatgt
    17041 cgtaaaaata actaagaata ggctcaaaca aagaatgcct tgaaaaattt gtttgtttaa
    17101 tcggttgatt tttttaatca gaggcgtcgt ttgttgttcg accgattgca atgcttgatt
    17161 gattttaccg atttctgtcg tgtttcctgt ttctacaacg acaccgactg cagaaccaga
    17221 ttgtacaagc gttcctgaaa aacccatatt tttctggtcg ccgatcgatt cttcttctgt
    17281 caaaacggca tgactttttt cagatgcttc tgattctccc gtcaaaatcg attcttccac
    17341 taataaatca tgtgcttcga ttaatcggat atctgctgga acgacatctc cggcacgtaa
    17401 gacaatgaca tctcctttta ccagttcttt cgcagggatt ttttcttgca tcccgtttga
    17461 tagtactatc gcttcttgtc ccatcagctg cttcaaacca ttcaatgatt cttccgcttt
    17521 tctttcttgc cagtaactaa cgagtccatt gacgatcact acgaataaaa taatacttcc
    17581 ttcaatatat tcactagaag caaatttcaa aaaagaggca atgatcaaca cgatcatcag
    17641 cagatcagta aaatgctttg ctattttttt ccatcgtttt gtctgctgcc ttgcttttat
    17701 ttcattatat ccttgatcag tcaggcgttt ctgtcgttct tcttgcgaca agccttctgt
    17761 tgatgtttga tagtttttca atacatcttc taccgtcaat ctgtaccagt tcatcctatc
    17821 ccctcctgtt taattgatac tgttattata cgcagggaag ttttttcagg cgtatcgatt
    17881 atttcagggg ggtctgaaat aattcgtttt gcaaaatgta tctgggccta actgatattt
    17941 ctattgctta ttcttttata tacaaaattt aagaatacaa accagataac caataaaaaa
    18001 ggaataaagc gtgtcgtgct ttcgaataac aagctgaaag caattgaagt aaaaaacacc
    18061 aacaaagcaa gatctttata tctttgtgta gtgttccgtt tcttttcttt aataaattga
    18121 acatgtgcac acaagatcga agaccacaca aatagaaaac aactcgttgt catgcctgcc
    18181 aaaagctgaa aaaacaatcg actatcagct acaaaaaaat gtagacaaag accggcaaaa
    18241 aataacattc ctgtaattaa aataccgtgt aaaggtacgc ctttcttaga aagcgcacaa
    18301 aaccaatggg gcaaactttt ttcttttgct gtttgatata aaatcctgct agtactgtag
    18361 actgcactat ttgctgagga caaagaagca accaaaatca ttagattgac aattgctgct
    18421 ccgccttggc aatggatcac ttgcagcata gtcactaaag gactttcttt taaatctaaa
    18481 caatgccatg gaacaacaaa taaaatcgac aaaatcgttc ctatatagaa aaagagaata
    18541 cgccaaggca gacagttgat tgcttttttc aaattctttt gcggatcact tgtctcgcca
    18601 acggttaagc cgatcatttc aattccagca aaactgaata aaaccatagg aaatgagact
    18661 accattccag caaagccaaa cggtagtaat tcatcgatct gtccaaaatt ttctttgatt
    18721 ccgatagact gggtatgaaa atctgagaca ctcaatccta tacccaataa gataaaaagg
    18781 aaaatcccca agatcttgat gattgagaaa aaagattcca attcaccaaa ccacttgact
    18841 gcaacaaggt tgactgccat taagatcatt agtgcgctac ctccgcttat caaagctggt
    18901 acatgtacaa accagaaatg gatatacaat cctattgctg ttaattccgc aagtccagct
    18961 ccaagccagc aaagccaata agtccaattg acaaaaaatg cccacttttt gcctaaataa
    19021 tagtcaacga aatcactgat agagttaaat gtactatttg ctaaaaataa atcacccatg
    19081 gcccgcatca aaccataaat agccaaaccg catatcaaat aaacgagaat aacagctggc
    19141 cctgctgatg caattgcctt tgctgaacct aaaaataatc ctgttccaat tgcgccacct
    19201 aatgcgatta actggatatt tcgattagtt agagtccgtt tcattgccgg tttttccatc
    19261 aagttcacct ttttcttttt caagcagcat ggcgacttcc tgttcccaag aaggtttctt
    19321 tattttttca atacagagtg gtactacaaa caaaatgaca ctgatggctg tgactgcaat
    19381 tagattgatc catgttccag ctttcatcgt cagtccgaca gaaaatccga ttgccgcaaa
    19441 taaaacaata acgacagtcc aaactccaag attgctcttt ttctcttttt ttccaattct
    19501 aaatggtcga ttcaactttg catgtgtata acgcaatctc aaaactgcga tccccatgat
    19561 gacatagacg atacagtaga taacagtagt tgaatttacg agcatcaaaa atgcttgatt
    19621 gacgccagga atcagcaaat aaaaaagtgc aaagattgag ataatgacag actgagtcaa
    19681 aataacggat ttggatacgt caaattgatt cgtcttccaa aaattgaatt ttggtggata
    19741 cgcacctttt ctagcacttt ctgtcactgt ctttgctggt cctgaggccc aagcagataa
    19801 ttggactgct acaccaatga agacaagtaa actaaacaga ttcacaataa gatgtggcaa
    19861 accaagaatt tggcaataaa tcgaaataga ttgcgcgata ttattcaatt ccatttgtcc
    19921 ttttggtacg acatttgcta caactaaagc attcaaaata ttgacgaaaa atataaaaat
    19981 taacgcggca aatactccgc gaatatacgt tttaactgga ttttccaatc gagtgatata
    20041 cacagaggac atttcgatac cagtaaaaat aaacatgatc ggcgcaaaat aaacaaatga
    20101 ggatgcagtc gtagtatctg gtattaattt gtcccatgaa aaactgccta atgttccggt
    20161 tggtacgatc ccaactttga tccacgcagc aaaccctaat aagagcatca tcgtcaaagg
    20221 cacgtacaat cctaaccaaa caccgacttt tccgccgatt tttgccatat caaatttcaa
    20281 attcaaaaaa gtgatgatcc aataaacgac taaaatgaca actaatgtaa atttgctgtt
    20341 caaaccaagc ggcacattat cgataatatt accaaacaac ggagcgagtg ctgaagcgac
    20401 catcaccatc ccaggaaaca tttggaccca taacagccat gagacaacga acccccattt
    20461 ttttcctaga gcattcgata cccaaagttc tggaccacct ttttggggaa acattccagc
    20521 aaattcacca gagatcaatg agattggaaa agcgtacaat aatactgcaa ccagcatgta
    20581 aaaaatcatc gtccagctcg ttgctgctac atctggaata tttcgtacac ttgctacaag
    20641 ggcgcaggtc attccgataa aggtcatggt cgtgatttgc tttgatgaac cagtatcttt
    20701 catctttatt ccttctttca atagcgacat atgtttttgt tttcttttat ttttgttcaa
    20761 gaacagaaat gacaaggttt catcgattca gtacaaaacc cagtatcacc gaaacatcca
    20821 ttgaacgatc ttagtgaact gggtttgata catgaaacga acgatccaac ttatagaacg
    20881 ttttataaaa aagaatatct tggcgaggaa tatatgaaaa acagggggaa atacaattgt
    20941 tttgatgaag caaataattg tctgttctcc atatgtctct tgttattctt tcacacaaaa
    21001 atcagttcta ttggatgatt tcgtttagtt tcttttccat tgctttggcg attttttttg
    21061 tataaaaatt gaaaattcga tcatcattca aataaggagt catgatagcc gcacgtagca
    21121 gagtgatttt ctgttctttt ttccattctt ctttcgttag acccattctt tcaacaaaac
    21181 gaatcggaga atcaccgtaa tcttcttgtg taaatgtagt atgagaagta ataaaccgtt
    21241 caccataaac atcgccatcc atataagaag accgatcgaa cattttttca tttaattcat
    21301 tgatagcgtt caaatctgta cagccttgtt ctttgaatac ccaattgacc atattaaaat
    21361 caggatgatt caatggatag acttcgattg tttttccttt aacagtaaaa gtcagatgat
    21421 cgagaaattc tctgaatctt tgagctgcct cgattgaagc cccaatcaat tgaccatatc
    21481 cggtcacatt caatggcaat acacgatgtg ctgtccagac tgcggcggcg gttgctccag
    21541 cttttgatcc ttccaaaata taagctccta acatatctgg agcctttact gatttttcaa
    21601 acacatatgg cgcgaaataa gaaatgatat ttctcatatt tttatgtttg attacgattc
    21661 ctccagcagc ataagggaca tagcccatct tatgaggatc tatcgtgaca gaatccgctt
    21721 ctgaaatagc tctgaaccct tcatatacct ctttgtcaat cgttacgcag tggtggaaaa
    21781 catgatgttc ttcaaagaat tcagctaagg aagcatacgg cacaaattct cctgcttcgt
    21841 ttagaaacag tgaacgcgca tagccgccat atgcggcatc aacatgtaaa tagaaataga
    21901 tcccttcatc ttttaacctt tctcttaatt ggacgatttt atctacactg tctacttgac
    21961 cttcttctgt cgttcctacg acagcaacca cacctagaat tgggattttt tgtccagcta
    22021 attcacgaat tgttttttcc aaagcattaa tatccattcg ataatcctct tgaactggga
    22081 tagcaaccat ctgatctaag ccgactccgc aaatatctaa cgccttcatc caagaatagt
    22141 gttttgtttg agggaccagc catttcccta atctttggat attttttccg ctacgggaag
    22201 aagctgcttt gacctcatcc atttcttcgt ccgtaaagct ttcggtcatt tccaaaattt
    22261 cttcaacaga taaattcaaa agttcccact cagacatttt tttcactttt tcaggcagca
    22321 cttccttgac agctaaagga atcgatttga tgcaacgggc gtaccataat ccttcaagat
    22381 tagcaataga accatctgct gcaatgtgtc cccatccgtc tgtcatattg aataatgaag
    22441 caaaatcctg tcctacttct gcttccattt gcgaagttgc catagaagat tccaatgcaa
    22501 cattatttgg gttccacagc atcgcatagt tgtaagctaa taaagcaggc atcaatgttt
    22561 cagcattcat ttgtccccag taacggcctg ctgaatgcca tgggatcgat cctgcgcgta
    22621 atcgctgact gacttcatcc aagacctcta tcatatggtc gcgggtggct agataatctg
    22681 gagtgagctt atctccttcg ctaattgcag gcatatctga aggaatataa ttttctctcc
    22741 atccaagatg ttcatctacc atcttgttca acaacatttt ataaactggt ccattttcag
    22801 ctttgtcgcc tataaagacg gccttgatat ccatatcttt catttttctt ccctccatat
    22861 tttgttggtc aatgtcttgc agatacatgt gtttatctgt aacacctctt cactatagcg
    22921 ccaaaaatga gaattttttt tttataacat caaatccacg tagattttta caaacaaaaa
    22981 taaataaagt cggatttttc tgataattca acgcaaaaaa gcgaacaatt tttcaataaa
    23041 tcagtcttct aaaatagata acctaaaaaa agcgaacaaa caaataaaat gactgtcctt
    23101 cattcactaa acatattttt atttttttat attgttaaaa attcgcaatt tattttttaa
    23161 tagaattatc tgttttccaa attttataac ataaaaaacc ttttttaacg gcatttatat
    23221 gctttttcag aaattaactg atgatttcaa aaagaaaaca tgtcataata tttcccatat
    23281 tgtgcaaaaa aatacaaggc ggaggctcta ctatgattga agaatatatt gagaaaaata
    23341 ttttaagaca gctgttttta tgtgggcaat tctatgtaaa taaagaagtc aatctagaaa
    23401 agctctccaa tctcctgcat gtttgcaaaa ccactttact caatgatatc aataacataa
    23461 aaaaagaatt tgaagaacag atcgcttata cacatcggga aaaggaccgt tacactttat
    23521 atttttcaga acatatacct cgatgcaaga tcatgcagca attgtctcaa aattcgttgt
    23581 ttttaaaaac ttgtctgctt tatttggaag aagatgaacc tgattatctt caattaactg
    23641 aatgtgaatt catctctgtc agcaaagctt attcattaaa aaaacaagta ttagcttatt
    23701 ttaatgactg cgggatcgaa atcgaccgct actcccctcg attcactgaa atggaaagac
    23761 gtctgctgct tcttaatgtg tcgtatcgtt taggcggttt taattcttgg gagctacctg
    23821 aatcgttttt tgaacgtgcc gatcgtttca ttgaatccgt aacggaaaat agtggcagat
    23881 tttatgataa agaaaataaa gaaatcttat cgattggttt tgctatcagt ttcctacgcc
    23941 agcaagtgtg tgctgtgaca atagattcaa aatttatcga agaaatcaaa aaacgtccta
    24001 tttacaatta tgtcgaatca gcatgggaaa acactgattt ccaaacctat tataaaaaag
    24061 aagaattcgc ctttattttg acactattca atctttgtaa ttatggtttc cattcttatc
    24121 agttgattgc ggaagacttt caacagctac accaagtgtt tattgacaat actcccgaaa
    24181 taaaagaact agttgctacc tttgaatctc acttcaatca agaattattc ggaaaccaac
    24241 cgttcgaacg tgcgctgatt catctgatgc gttcggcttg ggataactat caattattca
    24301 tgccagaaaa attttatctt ttaaatgagg aacagacaaa tttatataaa gaagtccaga
    24361 caattttttc cagctggtcc agccagttac cttatgatct acggttgaat cctaattgca
    24421 tgcgggcttt tgtgatcgaa ttgagcggaa ttctccgtct gacaaaagaa catctgacta
    24481 tctatatcgt cacaaattcg gatgttcatt atctgattta tcgggaagcc ttggaagctg
    24541 taactacttt cgattttcaa gttgcaccaa caatttattc aagtatatct gatataaaga
    24601 agtatgcgca gcagtcctca aaccgggtat tatgtgaacg tacattatat acaccagatg
    24661 cggtccaata tgaaaatatt attcctattt cgatcaatac gatcgatcga gctatcattt
    24721 cagctgtgca aaacaaataa ggggctgtga caaaaatcat gtcacagcct cttatttgtt
    24781 gaagatgccc atttctgtaa caatctcttg atacacgtca ggctcacaac ctcacttatt
    24841 ttggacatct acgggtccga tctctttatt taaaaaataa gaaatgatgg ttcggataac
    24901 taccaacgcc gctagttgga aaatatcctg caaggtaggt ttgataatcg attcgatgat
    24961 cccagctgaa acaagaattt ccaaacttag aagtatatag cctcccaaca tctttttaat
    25021 agcattgttc tcgctgactc ggtcgctttg cacttttgta tggacaaata aatatttcaa
    25081 aaaatccttg attgatagac aaacacccca aaccaataaa cagatagcaa agatatcaag
    25141 tcccagtaca agtttttcga aatatggaag aagaaactgc atcaactctg tcgtcatcct
    25201 acctctcctc ttctttgttt ttctttgtct tctatttcct gattcaagaa ataagaaatg
    25261 actgttcgaa tgataataat aagtgcaagc ttcaaaatat ctgtccaagt cggcttgata
    25321 actgactcga tgatatcagc aacgactagt acttccaagc ttaacaaaat ataacttgct
    25381 agcagttttc ttgtatctgc attacgagta ttacgttctg catagttttt cttgacatga
    25441 gaccaatcaa aaagtttgaa tagaacaaga acgatcccga caattaagat accgatagaa
    25501 caaatattta aaatagcaat gatccattct agaaattcaa ttaaatttga gcgaaacata
    25561 tacgctacct actttctttt aatttgttcc tattctttta ttgtaaaaga aagttacctt
    25621 gttcgtctat ttaaaagttg ttctaatgat aacggttcgc ttaaattttt ctttttcact
    25681 ctcccatttt tctccctcca tccataaatc gtggaagtat ccaaaacaac gtagcaagtt
    25741 aaaataaggc ttagtccgtc atcagctttt caaagaagca ctgtctcggt gctttttttg
    25801 tacccaaatt tctaaaaata gaaccataca cctcctcaaa tcttttcttc ttacctaccc
    25861 tatttttatt tacaggaaaa tcagacactt cttctttttt gtcaaaattt cctttttctt
    25921 ctcgtcctat catttggcat tctaataaaa acgaactatc cttggatata gaaagagaaa
    25981 acaaggaggc gtttttcatg agtaaaaaat taatcaaagt cggcatcggt ttaggtttgc
    26041 ttgcccttgg cgctgcatat ctaggaaaaa agacaggact attcgaagat gacagtcatt
    26101 tatatgatga attcgaatca atctagcatc ctatttagtt gaatcactac ggacatactt
    26161 ggaagacatt ggaggaatca caatgagaaa aagtaaagca ataatgattg gtgccggtat
    26221 tgccaatatg gcagcggctg tttatttgat tcaagaaggc aaatggaaag gggatcagat
    26281 caccttttat tcattggatg atcacggttc aaatgatggt gcaccgaccg acactgtagt
    26341 ggatgaatat tggaataaaa accatccatt agaaaaccaa aaaggctata ttgctcgagg
    26401 cggtcgcatg cttaattacc gtacctatgt agatttgatg gatctgttaa gtcgaatacc
    26461 ttctgttact gagccaggga tgactgctga agaagatacg agagattttg atgcaaaaca
    26521 ccgaaccttt gacaaagcac gtttgatgga aggtggcaaa ggaatcattg atggcggaaa
    26581 actgggattc aataataaag accgtattct tctgactaaa ctgattgcta tgcctgattc
    26641 agaagaagaa aaactagaca atatcactat cgaagaatat ttcaaagatg atccacactt
    26701 ttttgaaaca aacttctggt ttatgtggga aacaacattt gctttcagaa cacgtagttc
    26761 tgctcaagaa ttgcgtcgct atatgcatca aatgatttat gaattcactc aaatcgaaca
    26821 tctagtgggc gtcaatcgaa cacgttacaa tcaatacgaa agtattatgc ttcctttgat
    26881 cgagtacttg aaagaacaag gatgcaaaat cattttgaac cgccgtgtca ctgactggga
    26941 attcaaagaa acaccgatgc aagatgaaat cactgtgact ggtttgaaaa ttaccaatac
    27001 actgactcaa gaagaagaat acgttgtggt cgatgaagat acagcagtta ttttcactaa
    27061 tgggtccatt acagattcag ctactttagg agattacgaa acaccagctg tagaaaatat
    27121 ggattatggt gcagcttcta gtctctggaa aaaagccagt gaacgtttct acaacttggg
    27181 aaatcctgac aaattctttg ctgatcgaga tgcaagcgaa tgggtcagct ttaccctgac
    27241 gacgaaaaac catttgttgt tgaatgaaat cacccgtatc acgactcaag aaccaggaaa
    27301 tgccttgaat tcatttattt caaccacacc gatcacgccg ttaggccaaa aagatgtcaa
    27361 tatgtctatt gtcgtgcatc atcaaccgca ttttacgtct cagaaaccaa atgaaacagt
    27421 tatttggggt tatttccttt atccaagacg acgtggcgaa tttgtggata aacagtatat
    27481 taaaatgaat ggaaaagaaa tgctagaaga actgattgga caactttcaa aagtagatcc
    27541 tggaccagtg aatattcgtg aaaaagaaac agaaattttc gacagcgtaa taaataatat
    27601 ccctgtctat atgccttatg cttccgcgtt gttcaataat cgtgccaaaa gcgatcgacc
    27661 aaaagtcatt cctaaacatt cgaccaatct ggcctttaca ggagaatttg tcgagcagcc
    27721 ttatcagatg atctttactg aacaaagtgc tgtacgttct ggagaaatcg ctgctttcca
    27781 ttttgcgggt attcctatgt cccgtcttgt caaaacaccg cgttacgaca aagatattcc
    27841 tactctagct cgtgccgcaa aaaaaatgtt tgaataatca agaggttgtg aaaaagctgc
    27901 cctgcatcaa gtaataagaa caaccaggct aaaacagctt cttatgtttt tgatttggtt
    27961 ggacttatta ccgcagatgc aagcttttga acaccgttta ccaagaggtt atgaaaaagc
    28021 tgccctgcat caagtaataa gagcagccaa gctaaaacag cttcttatgt ttttgacttg
    28081 gttagactta ttaccgcaga tgcaagcttt tgaacaccgt ttaccaagag gttatgaaaa
    28141 aggcgtttag tttcgagtat gaagaaaaat tttgtgaaaa tggttcctca cgtttttgca
    28201 taaatttgac ttaataccga gaaactgatc ccttctgtca taacctcttt ttatttatgt
    28261 ctgtttcttt ttgaaaacaa ctattttttc aacgtttact ctcttgccaa tccaacatca
    28321 tctaacggat acaactattt caatgattac cattacacta aaaaatcgga aacacacctc
    28381 tccgaacaca aatacataag actatagaat gtttcattta agaaaaaaag cgtttcatta
    28441 tcaaaaaggt aagatctcct ttaaaatgaa attatctgta gtactaataa tgcagacaat
    28501 tttatgaatc tgaaaggagc atctccaatg aaagcagtca cttggcaagg aaaacagaaa
    28561 atggaaatca gaaacgtaga tgatccaaaa atattagaac caacagatgc aatcatccgt
    28621 atcacagcaa cagccatttg cggctccgat ctgcatcttt accatcatgg tgaagcagtt
    28681 ttggaaaaag ggtatgttgt aggacacgaa ccaatgggaa tcgttgaaga agtgggatct
    28741 caagttcaaa cattgaaaaa agggcaacgt gtggttatcc cttttaatat tagttgtggt
    28801 cattgtcatt tctgcctgaa tcacatggaa agccaatgtg atgaatcaaa tccagatcag
    28861 ttatatggcg ggcttttcgg atttggcaag ttgaacggta atcattgggg tggtcaagcg
    28921 gaatatctaa gagttcctta tgcagactct acttcatttg ttgtaccaga taatgatctt
    28981 cctgatgaaa aagttttatt tctatcagat attttgccca cagcttattg gagcgttgaa
    29041 aacgcaggag taaaaaaagg cgatacagtt gtgattctag gttctggtcc agtcggtcta
    29101 ttcgctcaaa aattcgctgt tatggctgga gcagagcgcg taatcgcagt agatccagta
    29161 cagcaccgtc tagataaagc ggctaattac aatggggttg aaacttattt cttagaagac
    29221 acggcacaag ctggaacaga attgtatgaa ctggctaaag gtggcgcaga tgtcattatc
    29281 gattgtgtag gaatggacgg attagagcct gtcaaagaaa aagcgaaaaa tttagtaagc
    29341 ttacaaagcg gcacaatctc tccattacag atggcatcgc aagctgtcaa aaaatttgga
    29401 acagtccaag taactggtgt ttatatgact ccagcttctt cttacccgct gcaagaattt
    29461 tttatgcggg atatcgctgt caagcatgga caggcaccaa ttatccatct aatgccaaaa
    29521 atttacgcaa tgattgcaga aggactattc gatcctacac aaatcattac tcattcgctg
    29581 ccactatcag aagcagcaca agcatatgag atctttgaca aaaaagaaga taaaaatata
    29641 aaagttgttt tgaaaccttg aattcagtaa aaaatacagg tgattttctt tatgcccaaa
    29701 atcaaacgaa atacccatga gatcgaaata ggaaaagaag aagatatcga aatacgagga
    29761 cgtcgcttcc gcttctattt ccaaaatcgt tacacattga tctcactggc tgtcgattta
    29821 ttaaccggga ttttctatat ttttggaagc cttgcttcct taactaaaat ccccgatcga
    29881 tatggcacct atttttattt ggctggtgct attttcttga cgattcgtcc tattctcaga
    29941 atcgtaagga acatttttat ttacgacgaa aaaaaagcaa tgaagagaac aagcaaaaaa
    30001 aaagaggcat gagaattttt caaatctcat gcctcttttt tcaccctatc gtatcacttt
    30061 gatacagacg agcggtcgtc ccactttctt ccttgaccat tgtcaaaaat gtccactcgt
    30121 atcgttcata aaacgtggta tgatctgtaa ttaagtaaag tttttcgtac ccaagttgat
    30181 gcatttcttt tctgatttcc gctaatatcc gcttggctat cccctgctta cgcttttccg
    30241 gttcaacaaa cagggcacaa agatttggtg tcagatcagg gcggttatgg aagtcgtttt
    30301 caatgacgcc cgctcctccg acgatggttt ctttttcatc taagaccaca taccattgag
    30361 gaatactttc cttattagcg attccagtac gaatactttc ctcatacaca acagcaggaa
    30421 ctccccattt tgatgaaaac caattcgcgc tttgttttac taattctggg tgttctctta
    30481 atttgatcca gttatatgta accaatgttt aaacctccga ctaaaataaa acgtttacaa
    30541 aaattataca agtctttatg aaaattagca atgacaacaa tagtgatgta aaaaaaaaaa
    30601 cggaaaacaa tcacttgttc tccgtttttt gattaatcaa gcatctaaag cttcttttct
    30661 tctttcttgg atcgtagcta ccttgacgaa cggtagatag atcaaaatag aaagaaccaa
    30721 attgacagcg gctagtacac cacctgcaat actttttgtt gccaatacac cgccaataat
    30781 tggtggagtt acccatggag gcataacggt agctgctggc actaaacctg tagaagtcgc
    30841 aaagtaagca acactaacta
//
LOCUS       DACDUU010000028        29710 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.028, whole genome shotgun sequence.
ACCESSION   DACDUU010000028 DACDUU010000000
VERSION     DACDUU010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.028"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            356..631
                     /locus_tag="IWL30_001320"
     CDS             356..631
                     /locus_tag="IWL30_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706097.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            644..2722
                     /locus_tag="IWL30_001321"
     CDS             644..2722
                     /locus_tag="IWL30_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP9706098.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2882..4768
                     /locus_tag="IWL30_001322"
     CDS             2882..4768
                     /locus_tag="IWL30_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9706099.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4780..5727
                     /locus_tag="IWL30_001323"
     CDS             4780..5727
                     /locus_tag="IWL30_001323"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP9706100.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5747..6274
                     /locus_tag="IWL30_001324"
     CDS             5747..6274
                     /locus_tag="IWL30_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9706101.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6337..6990)
                     /locus_tag="IWL30_001325"
     CDS             complement(6337..6990)
                     /locus_tag="IWL30_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP9706102.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7122..7964
                     /locus_tag="IWL30_001326"
     CDS             7122..7964
                     /locus_tag="IWL30_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9706103.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8122..9018
                     /locus_tag="IWL30_001327"
     CDS             8122..9018
                     /locus_tag="IWL30_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP9706104.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            9021..9734
                     /locus_tag="IWL30_001328"
     CDS             9021..9734
                     /locus_tag="IWL30_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP9706105.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9754..10272
                     /gene="msrA"
                     /locus_tag="IWL30_001329"
     CDS             9754..10272
                     /gene="msrA"
                     /locus_tag="IWL30_001329"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9706106.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQDYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10269..10496
                     /locus_tag="IWL30_001330"
     CDS             10269..10496
                     /locus_tag="IWL30_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP9706107.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10648..11199
                     /gene="lepB"
                     /locus_tag="IWL30_001331"
     CDS             10648..11199
                     /gene="lepB"
                     /locus_tag="IWL30_001331"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP9706108.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11282..11824)
                     /locus_tag="IWL30_001332"
     CDS             complement(11282..11824)
                     /locus_tag="IWL30_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP9706109.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(12061..12459)
                     /locus_tag="IWL30_001333"
     CDS             complement(12061..12459)
                     /locus_tag="IWL30_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP9706110.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12719..13579
                     /gene="ylqF"
                     /locus_tag="IWL30_001334"
     CDS             12719..13579
                     /gene="ylqF"
                     /locus_tag="IWL30_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP9706111.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13572..14339
                     /locus_tag="IWL30_001335"
     CDS             13572..14339
                     /locus_tag="IWL30_001335"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP9706112.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14395..15252
                     /gene="dprA"
                     /locus_tag="IWL30_001336"
     CDS             14395..15252
                     /gene="dprA"
                     /locus_tag="IWL30_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP9706113.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15374..17452
                     /gene="topA"
                     /locus_tag="IWL30_001337"
     CDS             15374..17452
                     /gene="topA"
                     /locus_tag="IWL30_001337"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP9706114.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17421..18797
                     /gene="trmFO"
                     /locus_tag="IWL30_001338"
     CDS             17421..18797
                     /gene="trmFO"
                     /locus_tag="IWL30_001338"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAP9706115.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            18995..19900
                     /gene="xerC"
                     /locus_tag="IWL30_001339"
     CDS             18995..19900
                     /gene="xerC"
                     /locus_tag="IWL30_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP9706116.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            19937..20485
                     /gene="hslV"
                     /locus_tag="IWL30_001340"
     CDS             19937..20485
                     /gene="hslV"
                     /locus_tag="IWL30_001340"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAP9706117.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            20499..21899
                     /gene="hslU"
                     /locus_tag="IWL30_001341"
     CDS             20499..21899
                     /gene="hslU"
                     /locus_tag="IWL30_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAP9706118.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            21917..22708
                     /gene="codY"
                     /locus_tag="IWL30_001342"
     CDS             21917..22708
                     /gene="codY"
                     /locus_tag="IWL30_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAP9706119.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            22790..23665
                     /locus_tag="IWL30_001343"
     CDS             22790..23665
                     /locus_tag="IWL30_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP9706120.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(23797..24435)
                     /gene="plsY"
                     /locus_tag="IWL30_001344"
     CDS             complement(23797..24435)
                     /gene="plsY"
                     /locus_tag="IWL30_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAP9706121.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            24562..24996
                     /locus_tag="IWL30_001345"
     CDS             24562..24996
                     /locus_tag="IWL30_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAP9706122.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            25162..27207
                     /gene="parE"
                     /locus_tag="IWL30_001346"
     CDS             25162..27207
                     /gene="parE"
                     /locus_tag="IWL30_001346"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP9706123.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            27219..29669
                     /gene="parC"
                     /locus_tag="IWL30_001347"
     CDS             27219..29669
                     /gene="parC"
                     /locus_tag="IWL30_001347"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAP9706124.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDRSIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
BASE COUNT        10347 a         5121 c         6329 g         7913 t
ORIGIN      
        1 ttaatcatac tagagaacga tatgtttttt aatacctaaa ataaaaatgg ggctgaagaa
       61 tcaattctga ttcatcagtc ccataaatta tagagccgat ttttgtcgca gccccatttt
      121 tttgttgtga taaaagaaca agaaaggcag gaatgggaaa gtaaacatcc ttttgtcaga
      181 aacttttatc tttcttaagt tttacttgat atctgatact atttggaata gaatgaggcg
      241 ttgaaaaatt tatggaaatt tctaactagc aataaacgat gaaacaatga aatatttttc
      301 atccaaaaag aactttgcta taatgatgga agtttgtgta taggagatct ggaaaatgag
      361 agagagtttt gaacaacaaa aaaaactgtt gcatgatcgc tatggcgccc tttcgatgga
      421 tgaccgccgt caaatacttt gtaagttgcg taaaagaaat atattgatgt atcgtcagtt
      481 ggaacgtctg aaacatgatc tgcttcgttt ggagtcaaaa cgtgtacagt gtgaattaga
      541 ggggaaccaa acacaggttg aagtagtcga aaccaaaatt ttaaagaaaa aagaacaatt
      601 tttaaaaatg cttacacaaa ataaaaaata aaggggatgg acgatggaat ttgctgagtt
      661 agttattgta tttgcagtaa cgatcacctt atcgaacata gctagccgca ttttgccaat
      721 gattccagct ccactgatcc aaattttcct tggagtggta ttaggtctga cagagtgggg
      781 acaatcaatc gattttgaac ctgaattgtt cttagttatg attattgcac cattgttatt
      841 tagagaagga gaaaaagcag atatttcttc gattctcaaa aatttcggga caattttgtt
      901 cttggcattt ggaggagtca ttttgaccct tgtcggggta ggtgcgacgt tatcattcct
      961 gttgccaagt gtcccacttg ctgcctgctt tgcttttgga gcagcgttag gtccgacaga
     1021 tgcggtagca gtcagttctc tttctggacg agtgaatatc cctaaaaagg cgatgcatat
     1081 tttggaagga gaaggtcttt tgaatgatgc atctggtgtt actgccttcc agtttgctct
     1141 tggtgcgctg atcaccggat ctttttcagc agtaaacgcg gggatgagtc tagtagtttc
     1201 tagtattggg ggagctttga tcggcttttt gcttgtctgg ttcaaacaaa aaataattca
     1261 tttgatcgaa aaagcatcag cacaagatgt cacagggtat ctgctgatcg aacttttgct
     1321 tccttttcta gcctatgtat tggcagaggc atttgatgta tctggaatca ttgctgcagt
     1381 ggcagcaggt attctccaag cttctggttt taggaaaatc tctgtgtttg atgcagaatt
     1441 gtcgagcttg tcccacagta catggacaac tattgctttt acactgaatg cattagtatt
     1501 tatattttta ggaatcgaat tgacgcaagt gttctcgcca gtatggggag acggtttata
     1561 tccaaacggt cttttattag cgattatcgt attaatctct gtcatgcttt ttgtgatcag
     1621 attcatttct atcagtcttt tttatgtatt caaagatggc agtaaaaagt tcaaaaaaca
     1681 gttgaacgag atcttgatct taacatttgg cggtgtaaag ggaacagtca gtttggctac
     1741 gatttttatt ttgccatttt caatcaataa tatgatgttt taccagcggt ctttgctttt
     1801 gtttctgacg gcgggtgtca tcttagtgac tttggtcata gggatcattg tcttgcctat
     1861 gctgacggaa acagaggaag cgaaaagcac ggatttgaat gctttgatga ttttagaaga
     1921 ggtcgttgaa gttttgcgaa aagaaataaa ggaaattgat cgtaatacaa aagaattttt
     1981 agccactgaa gcagtgatag aaaattatca agaacggata cgtgatctgt acttggaaga
     2041 tttaaccgat gatgaaaagc aagaagtaca agaaatacaa gcactgattt tgtcgatcga
     2101 acgggatgga ttggatgaaa gttatcgttc gggcaagctt tcttctaatg gttatcgttt
     2161 ttactcccgt tttctttctc gattcgaaca ttctatcaca agccagattt tgtcttttat
     2221 cggcttttgg ttcattgttg tccgtaggtt aatgcgaatc gttctccatc cgaaaatgtt
     2281 ttggcaaaga agatatgctg atcgtcagac atttattagt gatgaagata tccaagaaat
     2341 cagagagaca tatcaaaaaa atactcagtt gattattgaa agtttagata acttgactga
     2401 tatttatgat gaaacgatgg ttcaattttt tatccagcaa cgcaaacttg aaggaataaa
     2461 aatgaactcg gggaatttga tttcttcgtg gatgatccag caagatagtc tgtttacgaa
     2521 aaaaatgcta agaggttatt atcttgaaag aaaaatcatt gatgaatacg aagttgctga
     2581 aaaaatcacg acgttttctg cgaacagcta tcgcaggaat atcaatttgt tagaatctta
     2641 tacgatgaac aaaccatctg ataacttttc tttccgattt gcgtttcgag caaagtcaaa
     2701 aaaaacgagt ccaataaaat aaaaaggtaa aaattttcaa gttttataaa aaacaaaaca
     2761 tatcagaaca ggatcgaatc ggtcctgttc tttttttgag aacacaaaaa gaacaaatac
     2821 caaaaaaatt ttcctgaagt tcactatttt tatgatacaa tatccaagat aaggagggaa
     2881 catgttgaaa gaattaaaag taactgacct caaaaaaact tatggtgaaa aagatttgtt
     2941 tgaccagatt tcttttttgg tccacgaaaa agaccgtatc gggttgattg ggacgaacgg
     3001 gacgggtaaa acaagtctct taaatattat tgccggtatc gatagcagcg acggtgatcg
     3061 gcaaacagtg ttttatccaa ctgattaccg tattggttat ctttctcaag cagctgaatt
     3121 ttccgatgaa ttaactgttc tccaagcagt ctttcaagga aacagcccct tgatccaaac
     3181 tgttcgagca tatgaggaag cattgatcga actaggggaa aacggagaag accctgatgt
     3241 gcaaaaacgt tatgctaaag cagaagaaca aatgaataaa gaagatgctt ggactacaga
     3301 taccaacgca aagattattt tgcaaaaatt aggaatcagt gaattagata aaaaaatcag
     3361 tactttatca ggtggtcaga aaaaacgagt tagtctggcg caagtgttga ttgatgaacc
     3421 cgatttacta ttactagacg aaccaacaaa ccatttagat tatgaagcga tcgagtggtt
     3481 ggaaaactat ttgaaacaat accgtggcgc actattgatg gttacccacg atcgttattt
     3541 tctagataga gtagcaaacc gaatcttcga actttcattt ggaaaattat acgagtataa
     3601 aggaaattat gaagcttacg tcttagaaaa agcagaacgt gatcgtgtag aagtagaaca
     3661 agaagaaaag cgcaagcggc tttataaaca ggaattagct tggatgaggg ctggcgtaca
     3721 ggcacgtggc accaagcagc aagcacggat caaccgtttc gaagatctaa aggagaactt
     3781 gtaccaagta aaccaagaag atgatttaga gctgaaccta gcgacacaac gtttagggaa
     3841 aaaagtactg gaaatcaaag atggcagtta ccgtataaat gaccaaacac tgctagaaca
     3901 tttagattta ttgattcaat ctcgggaaag actaggtatt actggtaaaa atggtgcggg
     3961 aaaatcgaca ttgcttaata ttctagctgg cagaattccc ctggatagcg gtacaatgtc
     4021 tatcggtgaa acggttcatc ttgcgtatta tacacaagaa aatgaagaaa tggcaccaga
     4081 taaacggatg attgcttact tacaagaagc tgctgaagaa gcaaaaacag cagatggcag
     4141 tcaaatcgga gtagcagaat tacttgaacg atttttattt ccaagattta tgcatgggac
     4201 actgataggc aaactttcag gcggagaaaa acgccggtta tttttgttga aactgctgat
     4261 tcagcaacct aatgttttat tattggatga accgaccaat gatttggaca tagcaacatt
     4321 gacgattttg gaagattatt ttagaagttt tccaggtgct gtgatcactg tttcccatga
     4381 ccgttatttc ttggacaagg tagcagataa gttattagta ttccaaggaa acggaaaaca
     4441 agaactttat tacggaaaca tgtccagtta tttactgaag caaaaagaga cccagcagcc
     4501 tgctgaaaaa gcgaaaccaa aaatacagag caaggaacct gctgaaaaga agaaattatc
     4561 ttacatggaa caaaaagaat gggaaacgat tgaagatgag attgcagaac ttgaagaaaa
     4621 aatctcattg ctccaagaag agatgaatca tcaaggagat aatttcactc gattacaaga
     4681 attgcaaaac gacgtatctg aaacagaagc acaacttgaa gaaaaaatgg cacggtggga
     4741 atacttgagt gaatgggtag aagattagga ggaaaaagga tggaacaagc gtatcttgac
     4801 ttaggaagaa aattgttgga agaagggcat cgtaaagaag acagaacagg cacaggcaca
     4861 ttaagcttat tcggatacca aatgcggttt gatctgcaaa aagggtttcc tttgttaaca
     4921 acgaaacgag taccttttgg attgatcaaa agcgaattgc tttggttttt aaaaggagat
     4981 acgaatatcc gctatctatt acaaaataaa aaccatattt gggatgaatg ggcatttgaa
     5041 cgctacatca aaagtccaga ctataatgga ccagatatga ctgattttgg ccatagatgt
     5101 ttgacagatc ctgaattcaa tgaagaatat aaaaagcaga gcaagctttt ttgcgagaag
     5161 atcctaaccg atgattcctt tgctgaaaca tacggcgatc ttggacacat ctacggctat
     5221 caatggcgtc actgggaaac aaaagacggt ggctttatcg atcaaatcaa acaagtgatc
     5281 gaagcaatca aaaagactcc tgattctaga agattgatcg tttctgcatg gaatccggaa
     5341 gatgttccta gcatggcttt gcctccttgt catacgatgt ttcaatttta tgttcaagag
     5401 ggacggctaa gctgccaatt gtatcaacgc agtggggatg tgtttctagg cgttccgttc
     5461 aacattgcaa gttatgcgtt gctgactcac ctaattgcac atgaaacagg tttagcggtt
     5521 ggcgaatttg ttcacacatt aggagatgct catctttata tcaatcatat cgaacaaatg
     5581 aaagaacaat tgagccgaga aatacgcgca tttccgactt tagttttgaa tcaggaaaag
     5641 cagtcagtat ttgattttga gatggaagat atccaattag aaggatatga cccacatccg
     5701 gcgatcaaag ctcctatcgc tgtttagaat cagaaaggat gagaaaatgt tagctgcaat
     5761 ttgggcacag gatgaaaacg gattgattgg aaaagaagac cgtcttcctt ggcgtttgcc
     5821 aaatgatctg aaattcttca aacaaatgac agaagcaaat acactagtga tgggacgaaa
     5881 aacattcgaa gggatgggta gccgtccgtt gccgaatcgt cagacgattg ttttgacgag
     5941 agattcttcc tataaagcag aaggcgtgca tgtcatgcat agtgtggaag acgtgcttgc
     6001 tctggaaaaa gaaacagacg gtatcttttt catcgctgga gggtcagctg tttatcaaga
     6061 atttctgcca ttttgtacga tattataccg tacagtcatc catcatgtat tcgatggaga
     6121 tgcttatttt ccacctgttg actggtctga ttggtcactg atcaatacta gtcaaggaga
     6181 aatggatgaa aaaaatcgtt atccgcatca atttgaaacg tatcagcgta atgagaatcc
     6241 tgtaaaaagc gggacaggcg aattgacgat ataaaaaagc tatggataga ctagctgtta
     6301 tttagagctg tcccatccat agctttattt tttttattaa gtaaagaata aaacagagaa
     6361 gtacataaaa cctgcaccta acataacaaa caaatgccaa acaacatgca tatagcggat
     6421 agaattcaag ctataaaaga atgctcctat cgtgtaagaa agcccgcctg ctaccatcaa
     6481 aataacacca gtcattccaa gagagtgata aagaggaaca gcagccacga cacagagcca
     6541 ccccataaaa atatagatga tcgtagatac ttttgatact ctctcccgtt tatgcaacgt
     6601 caacgcttta tatataatgc caagaatcgc aaacaaccag attaatacaa ataaagtcca
     6661 acctagccat cctcggatac tcagcaaaca aaaaggagta taacttcctg caatcaaaag
     6721 aaaaatcgaa ctatgatcga atacttggaa cacctttttt gcttttgtaa aaatcaatga
     6781 atgaaacaaa gtagacgtta aaaataaaag aatcatcatc gatccataga tagcataaga
     6841 gacaatatgg agggaggaat gcaagtgggc accttttact aaaagaacca ctagtcccgc
     6901 gatactcaat ccagtcccaa ttccatgggt gactgcatta aacacttcat ttaatatttc
     6961 atacgttcga ttaaatttcg ttttttccat gaaaacggct cctttactag gtgtaattaa
     7021 tgaaaaatca ttatttgtct gttatactaa aaatagaaat tgtcttacat gtagtttaac
     7081 atgaataagg catcttgtta aagaaagaga gagagaaaat aatgacaaat gtaaaaatcg
     7141 taacagattc ttcatgtacg atggaaaaaa atgtacgcga tgctttaggc attcgtgtca
     7201 tgccgctatc catcatgatc gacggaacag tatatcccga tgatgaccaa ctagacggcg
     7261 aaagatttat ggaaatgatg gcgaaagcaa aaaatctgcc caaaacaagc caaccaccaa
     7321 ttggagaatt catctcgatg tatgatgagc taggagaaga cggcagtgaa gtgatttcga
     7381 tccatatgac aaaaggcttg agtggaacag tagaagcagc aagacaagcc agtaatctaa
     7441 caaaaacgaa agtaacggtt atcgacagtg ataccactga ccaaggatta tcttttcagg
     7501 taattcgagc agctgaatta gcaaaagaag gtaaaacagt agagaaaatc cttgaagaag
     7561 tcaaaaaagt acaagacaat acaaaattat acatcggaat atccacacta gataacttgg
     7621 taaaaggtgg aagaatcagc cgaacaactg gtttgctgtc tagtgttttg aacatacgcg
     7681 tggtgatgaa tttcgcccat tctgaattaa tccctgtaac taaggggcga ggaaagaaaa
     7741 cgttcgataa atggtttaat aatttgaaaa atgaactgaa agaattgcca aatgtgcgac
     7801 aaatcggtat ttcacatgcg gatgcaaaag aattggcgca agaattcaaa gagggtttgc
     7861 aagaattatt cccggacatg catatccctg tactgcatac gaatccggtt atcgctacgc
     7921 atactggtaa agacgcgttt gcaatcatgt attatgtaga ttagtcgaaa agaggataag
     7981 ccctcagcct gaaaggcttg cagcttattc tctttttctt tataaaatta ggaaaataat
     8041 tgttttcttt tttcataaag actacctttt tgataaaatt tttgataaca ttagaaccaa
     8101 tcgaatacaa gaggtgaaaa aatgaaatta acgaaacgac atttgatcct cgttttgttc
     8161 ccattattaa ttgcgatcat catctatgca ggtttagctg cttctatacc gaaagctggc
     8221 ccactaatga aaccagtaac tactgtgaga gaagatccaa ataaaaaaga aaaacttcac
     8281 tacatagcta tcggagattc gctgactgaa ggagtaggag atcaaaccaa gcaaggagga
     8341 ttcgttcccc ttgtcgcaaa tgatctaaaa gaccgttata atttgaccgc ggttgaaaca
     8401 gaaaactatg gtgtaaatgg tgaacgaagt gaccagatct taaagcgagt aaaaacaaaa
     8461 gaagaaattc gaaacaatat caaaacagct gattttatca cactgactgt tggcggtaat
     8521 gatttgatga aagttatcca aaataattta ttcgggttaa cggtcaattc ctttaaaaag
     8581 ccactgaaaa actatcagga aaatgtcaca gaactattgg aagaatttcg atccctcaat
     8641 aaaaccgcac ctatttacat tttaggcatc tacaatccat tttatttgaa ctttcctgag
     8701 atcactgaca tgcagacaat tgttaacaat tggaacaaag gaacagaaac ggtgacaaaa
     8761 aaaatgccga actgctactt tatcccaatc aatgatttgc tttatcaagg attgaatcag
     8821 gaagtaggtg tcacagaaga aggaacagat caaacaacta gcacgacaga aaatctaaat
     8881 ggcatcaaaa ataatgtgct ctatgaagaa gatcatttcc atccaaataa tttaggttat
     8941 caattgatgg caaatgcagt gagagatcag ctgatcgaga cacaaaacca atggttagag
     9001 aaggaggatg atcaataaac atgaaaacaa gatctgacgt atcttcaaac aaaacaaaga
     9061 aaacaaatta ctggaaattc gccttcctta tccttcttgg ggtagtactt ggttcgaccg
     9121 tatttttagg cagccgtatt tttgctaatc gggaaccaga actcccagaa ataccagctc
     9181 ttacggaaag acaaggagat cctgtcttaa cgatcaattc aaataaagaa aaagtcaatc
     9241 aaatcatcag ctttttcctt agtgagtatc aaaaagatag tgatattgaa tataaatttt
     9301 atttggaaaa tgaagcacta ctcaatggta ctttcgaagt actaggtttt cctatcgatt
     9361 tctatttata ttttgatccg tatgtgatgg aaaacggaaa tgtccaacta aaagctagaa
     9421 gtctttctat cggaacattg agtttgccaa tcaaagatgt gatgaatatg atcaagagaa
     9481 actataaatt accagattgg atcgaagtca atacagatga tctgactatc atgcttaggt
     9541 tggatcaatt ccgaatgcaa aacgggatgt atattaaagc agataaaatc gacttagtaa
     9601 atgatgacat tcggtttagt ctatatttgc caaaagatac ctctgatgct tcaaacaact
     9661 caaataccaa caaaactagc accagcagta ctgaaagcaa gacaaaagaa aataaaaaat
     9721 catcggatga gtagtattag agggggaatc ataatggaac gagcaatatt tgcaggaggc
     9781 tgcttctggt gtatgatcca gcctttcgac acgcttcctg gtatccatac agtgatgtca
     9841 ggatatactg gagggcatgt accagatccg acttatgaac aagtaaaaag ccaaacgact
     9901 ggacatacag aagcagtgga gattatctat gatccaacat tgatttcata tgaacaatta
     9961 gtagaacttt actggcagca aactgatcca actgacgctt tcggacaatt cgaggatcgc
    10021 ggtgacaatt atcgcccggt gatcttttat ttgacagaag aacagcggat cattgctgaa
    10081 aaaagtaaga aacagttagc tgaaagtgga cgtttcgaac agccaatcgt tacaaaaatc
    10141 gaaccagccc aaccatttta tctagcagaa gaagaccatc aagactatta taaaaagcat
    10201 cctgaaaatt ttaaaagaaa ccatgcacgc cgggctgcct ttatcgaaga aaattggagg
    10261 aacgaagggt gaatcgaagc ttttttcatt atttaatgac actgcgaggc ggtggcacac
    10321 atgatgcttt atctgttttc gctacagaag ccggtaaaga catccaattt cctaagcaca
    10381 gctctagcta cgaagaaatt tctaattatc tggaactgaa tgtcgactat ttgccgtcca
    10441 tggatatctt tgacgaagca tgggaaaaat atttagaaaa taatcatgac atataagaga
    10501 tagttttgta ctttatgatc aaaaaagttt tttgtccaat agcctaattg aaagaattat
    10561 ttttatttca gctatctagt cgcatttttt tgatattttt cttataataa agaaagaatg
    10621 aaaaaaagag ggatggattg atagagattg acaaaaaaac aacgctatat taatcgcttc
    10681 tggcttgtat tcaaatactt acttgtttct gtattccttg cgttcatgtt gcggggattt
    10741 ttattcatcc cggtaccagt ggaaggcaat tcaatggaaa acgtgttgaa gcaaggtgat
    10801 atggttgtga tggaaaaatt ttcggagatc agacgatttg atattgttgt ttttcaatta
    10861 gcagatggca cgatttatat caaacgagta atcggtcttc caggagaaaa tgtcagctac
    10921 caaaatgatc aattgaagat caatgggaaa gtagttaagg aaccttatct aactaaaaac
    10981 ctgaaatcag atcatgcaaa tgcatcctat acgacggact ttacactaca agagctaacg
    11041 ggacaatcca agcttccgga agataattat tttgtacttg gagataatcg aagagtttca
    11101 aaagatagtc gttcgtttgg cacgatcaat aaaactgata ttctaggtaa agcaagattt
    11161 gtctattatc cacttgatga aataaaatgg atccagtgag aaacaataaa gcgtgcagtc
    11221 tgttgagact gcacgcttta ttgtttaatc caaaatcagt ttactacctt agtacgtaaa
    11281 atcaaacagc tcgtttaaac acttttctag cctctaaagt tttgataaat gaataaacaa
    11341 ccattatttg aatagggatt tggatcatat tcctgatgaa tctacctaga aaaattactt
    11401 tgaaatctgc accaaacaaa acagacatca gccaagaatt gataaaaagg ctgactggga
    11461 tcataatcaa taattgggca gcgatgacgg aaggcaggtt tatcttttta tgatataaaa
    11521 ataagccatt aataaatcca ccaaggaaag ccgtgataag aaaagcagct tgaaacgttc
    11581 ctgaaccaaa tatcgtgaaa tttaaagtgt tcgacaacat ttcaagcaaa ccagcccaga
    11641 tcggtccata tagataaccc ataagcgcca tacttaaaaa tccgaatgtg atttgaaaat
    11701 aagcaggacc aatcgctatc ttgtataaga tgacatcaag ggctaataaa agagcagagg
    11761 tcgacaacaa tcttgcattc atttcgggca atctaaaaac gtcttttgca atcaaagtgt
    11821 tcaaaatatt ctctcctttg tcagttaata ataaaaggat accataaaat caccaatgtg
    11881 tcatgacaca ttggtgattt acatacactg gcatctaaca attaaaaaaa gggtgagaag
    11941 actgctttta taaactgtaa aaaagagatt gtacgaataa aaaaaataga ggcggagaat
    12001 acttatcgta tcatccgcct ttgttttttt gttataaaaa acgtcagatt agttatctgc
    12061 ttacatcttc atccatacgg gttctaatgt atttccgtct agatcttgga cttctaatga
    12121 aaacatttct tcttcaggca ttcccatatc tacttcgtgg aaagtaccac cattttcttt
    12181 tgcaatttga gcaaattttt ttacttcatc cgcattttca acactaaacg aaatcaaagc
    12241 accacttgtt tttttattat cagctatttc tttatctttt ataaattttt tataaaaatc
    12301 atgagagaga agcattatcc aaaaagtgtc atcccatacc attgaaccag cttcttcagt
    12361 agaaaattct tcattttttt taaaacccag cttttcataa aaagcaacgg aacgattgac
    12421 atcagcaaca ggaaaattaa caaaaaccat actattcatt tgtttcgtct cctttctgtt
    12481 ttgttcgtct atcatctctt atttaggaaa cgctgtcaaa aatttagatt catatataga
    12541 aaccaaaata aaaatttgca tcatcaaaaa agtgaagatg tgatagaatg aggactggtt
    12601 cactttcact taactaatag atggaatttc tttataataa aaaagtgaat ttatattgaa
    12661 aaagaaaaaa acaagtaaag ccggggaata ttccggtatg aaaggaatgg agagaacaat
    12721 gaccatccaa tggtttccgg gacatatggc aaaagcccga cgagaagttt ctgaaaaaat
    12781 gaaatatgtg gacatcgtat ttgaattggt agacgcacga ttacctttat cttctcgtaa
    12841 tccaatgcta gatcagatca ttcagcaaaa acctcgcctt gttttattga ataaagcgga
    12901 tctggcagat ccacaacaga caaagctgtg gcaagaatat tttcgtaaac aaggccatct
    12961 tgcattagct atcacggcac aagaaagtaa aggaattaaa ggtctagtac ctgcggctaa
    13021 agaagcattg aaagaaaaac gggcaagaga tgctgctcgg gggatcaagc ctcgcgctat
    13081 tcgggcaatg gtcttaggta ttccaaatgt tgggaaatct acgttgatga atcgactagt
    13141 aggcaagaaa attgcacaga caggaaataa gcctggcgta acaaaaggtc agcagtggct
    13201 tcgtttaggt aatgagttgg aattgttaga cacaccaggg attttatggc ccaaatttga
    13261 agatgaagaa atcggcaaaa aactagcgct gacaggcgcg atcaaagacc agcttgtcca
    13321 tttagatgat ttagcgattt acggattaga cttttttgcc cgatattatt caggaagaat
    13381 cagcgatcgt tatggtttat caatcgagca agaagaaaaa ttggcaccgg aattactgat
    13441 ggatattaca gcaaaacgtg ggtataaaga agactatgat cgtggcagcg aattagtcgt
    13501 gcatgatatt cgtcaaggaa aacttggacg atacacactg gatcgttgcg aagaaatgga
    13561 gcaaaaaagt gatgactgaa tccatccaaa gtatcaaaga acgattgaaa agcatacaga
    13621 gaatagatga tcctacacta gaatcattaa gaaatgatcc acgcaaaggc gtgcagcaag
    13681 cactggcttc ttttgaacga cgattgaaaa aggaagcatt aattaaggaa aaatatgaag
    13741 aaatgttttg ctttgaaaag caagggtacg cacaaggtca ccaatggatt gccggcattg
    13801 atgaagtagg aagaggacca ttagctggac ctgtcgtggc agctgcagtt atcttgccga
    13861 aaaatgagat catattaggg ctgaatgact ccaaacaatt atcagagaaa aagcgagaac
    13921 ttctttatca acaagttcaa gaaaaagccc tggcaatcgg gatcgggatc gttgatcagc
    13981 agacaatcga tgatgtgaat atttatgaag caagtaaatt agcgatgatc caagcagtag
    14041 aaaaattgtc cattgtacca agctatttgt tgatcgatgc gatgaccttg gatttgccta
    14101 ttcagcagga aaaaataatc aaaggggatg cacgatctgt gtcgattgct gctgcaagta
    14161 tcattgctaa agtatatcga gaccaattga tgaaagaata tgacgtgctt tatcctggat
    14221 atggtttctc gcataatgca ggttatggaa caaaggaaca cttagaagga cttgaacgac
    14281 aaggaattac gccaatccat cgtaaaagtt ttgcacctgt aaaaaaatat ttacaataaa
    14341 aaacagccgc ttttacgtat cttattagaa agagagacga aaggcggttt tatgatgtat
    14401 caaatagaag aaaatttatt gaaacttgct tatacaaaag gaatcagtag tcaaggaaaa
    14461 tgggcacttg ctaactggat gatgcagtat ccttataaag aaatcggatt tgaagacatc
    14521 attaagatag gtaaaataac caaacaccgg gagctgtttc gcaaaagttg gcaggaaatt
    14581 cacgctaact ggggcaaaat ccaatcgaaa cagtcgttta tcacttgctt tgatcccaaa
    14641 tatccccctc agctgcttca cttgacctat ccgccaattg tactgtttta taatggtgat
    14701 ctatctttac tttcttgtaa catgttaagc gttgttggtg gaagacacac ttctgcttta
    14761 gccaaaaaaa cagtttatta tttactaaaa ccagtgatag aagctggata tgtgatcgtc
    14821 agtggctgcg cgaaagggat agatacatac ggtcaccaag cagcaatctc tcatggtgga
    14881 cggacaatcg cagtcattgg aacagggata gagcaagtct atcctgctga aaatcgtgac
    14941 ttgcaagagg tgattgcaaa tgatcatctt cttttgagtg aatatcctcc tcaatccggc
    15001 ccgaaaaagc attgttttcc aatgagaaat cggattatcg cagctttgtc taaaggaaca
    15061 tgtgtctttg aagccaagaa aaaaagcggc tctctgataa cagcacagca agcattggat
    15121 cttggaagaa cagttttttc tgttccagga aatattttaa cagaacattc taccggatgt
    15181 catcatttga tccaagacgg tgcagtttgc acggtttcgg gtgaagatat cttggcagaa
    15241 tttaaagaat aacaccatac ttttcatttg cttttcttga caaacgtttt ctgaatggat
    15301 atgattagct acgatttgta tgaatcctct tttaatagta ggaacacgac aatggaaagg
    15361 agtccattca gtaatggcgt ataaatattt agtgattgta gaatcacctg caaaagccaa
    15421 gacaattgag aaatatcttg gacgaaacta taaagtaatg gccagtgttg gccatataag
    15481 agatttaccg aaaagtaaaa tgggtatcga ttttgaaaac aattatgaac cccattatat
    15541 ttctatacgc ggaaaaggcg atgtcatcaa aagcctgaaa gccgcagcaa aaaaagctca
    15601 aaaagtttac ttggcaagtg acccggatag agaaggagaa gcgattgctt ggcatttagc
    15661 gtacctactt gggttggatc tgaaagaaaa aaatcgggtg gtcttcaatg aaatcacaaa
    15721 agacgcagtc aaagcagctt ttaaggaacc aagaacgatc gatgtagatt tagtagatgc
    15781 acagcaagct cgtcgtacct tagacagaat cgttggttat tcgatcagtc ctattctctg
    15841 gcgtaaggtc aagaaagggt taagtgcagg acgtgtccaa tctgtcgctt taaaaattat
    15901 tattgaccgt gaaaaagaga tccgagaatt tgttccagaa gaatattgga gcatcgacgg
    15961 taattttaaa aaagctcgca agaaattcaa agcaaatttc tggggaatcg acggtaagaa
    16021 aaagaaatta ccagatgcac aaagtgtaaa agaagtcact gctagaattg atggaacaga
    16081 cttcgaagtt aaaaaagtag aaaagaaaga gcgcaaaaga aaccctgcga atcctttcac
    16141 tacaagtagc ctgcaacaag aagctgcccg taagttaaac tttcgaacaa gaaaaaccat
    16201 gatggtagca cagcaattat atgaaggaat cactttgggt aaacaaggaa cagtcggctt
    16261 gattacatac atgcgtactg attctacccg gattgccgat acagcaaaag cagaggccgc
    16321 agcatttatt gaagatacgt atggaaaaga attttctaga catgatcacc gaaagatcaa
    16381 aaatgctcaa ggcgcacaag atgcccatga agctgtgcgt ccaacgagtg tgcttcgtac
    16441 accagatgaa ctcaaacaat atttagataa agatcaattg aaactttata cattgatctg
    16501 gtcacgtttt gtggctagtc agatgacacc agctatttta gatacaatga aagtgacatt
    16561 agaacaaaat ggtgtcatct tcattgcgaa cggttcaaaa atcaaattca aaggattcat
    16621 gcaagtgtat gtggaaggta gagatgacgg caaagaagag aaagaaaata ttcttccaga
    16681 acttgaagaa ggcgatgtag tccaatctgt tgatattgaa ccaaaacaac attttactca
    16741 accaccagca cgttttagtg aggctacgtt gattcgtgca ctagaagaaa acggggtagg
    16801 gcgaccttcc acttatgcac caacacttga tacgatccaa agacgctact atgtaaaatt
    16861 gacacaaaaa agatttgagc cgactgaact aggagaaatt gtcaattcat taatctgtga
    16921 attcttccct caaatcgtcg atatccattt cacagcagaa atggaaggag atttggataa
    16981 aattgaagaa ggcacggaag cgtgggtcaa agtggttgat cgtttctata aaccgtttga
    17041 aaaagaactg acgaatgcgg aagaaaaaat cgaaaaaatt caaatcaaag atgaaccagc
    17101 aggattcgat tgtgatgtct gtggacatcc gatggtgata aaattaggga aatatgggaa
    17161 gttttatgct tgcagtaatt ttcctgattg tcggaatacg aaaccaatcg taaaagaaat
    17221 cggtgtgact tgcccagtat gtcatgaagg acaagtcatt gaacgaaaat ctaagaaaaa
    17281 cagactattc tatggttgtt cccgtttccc agaatgtgaa ttcacttctt gggataaacc
    17341 agtgggcaga aattgtccaa aatgtgacgg atatctggtc gagaagaaag tcaaaggcgg
    17401 taaacaagta gtctgtgtaa atggagatta cgaagaagac atccaaaaat aatagaaaag
    17461 aggttctttc attgaaaatg gtaaatgttg ttggagcagg gcttgccgga agtgaagctg
    17521 cttggcagat cgcacaagca ggagtccctg tccgtttata tgaaatgcgt ccagtcaaat
    17581 cgactgaggc gcatcacaca agtaattttg ctgaactagt atgttccaat tcattaagag
    17641 gaaacagttt ggctaatgca gtagggttat tgaaagaaga aatgcgccga ttgaattcgg
    17701 tagtaatcac ttcggcagat gaaacagcag tgccagctgg tggtgcctta gctgttgacc
    17761 gcgattcttt ttctgaaaca attacccgaa aagtcaaaga gcatccatta gtaacagtga
    17821 taaacgaaga aattactgaa ataccggaag gaatcaccgt agtagcaaca ggacctttga
    17881 cttctcatcc attagcagaa agcatcaaag cattcaatgg ttcagatggt ttttatttct
    17941 atgatgcagc tgcaccaatc gtagacaaag ctacgatcga tatggataaa gtatatttga
    18001 agtcccgcta tgacaaagga gaagcagctt acttgaactg tccgatgaca gaagaggaat
    18061 tcaaagcttt ccatgaggca ctaatcaatg ccgaagtggc agaacttaaa tcttttgaga
    18121 aagaaaaata ttttgaagga tgtatgccaa ttgaagtaat ggcacagcga ggattcaaaa
    18181 cgatgctttt cggacctatg aaaccagttg gattggaaga tcctaaaact ggcaaacgtc
    18241 cttatgctgt tatccagctg cgtcaagaca atgctgcagc ttctttatac aacatcgtgg
    18301 gctttcagac tcacttgaaa tggggcgagc aaaaacgtgt tttccggatg attcctgggt
    18361 tagaaaatgc tgaattcgtt cgctatggtg tgatgcaccg aaacagcttt atgaattcgc
    18421 cagaattgct gaaaccgact taccaatcaa cgaaacgcga agatctgttc tttgcaggtc
    18481 agatgactgg tgtagaaggc tatgttgaaa gtgcagcgag cggactactt gctggaatca
    18541 acgccgcacg cttagcaaaa gaaatggaac cagtcgagtt tccacaagaa acagctattg
    18601 gaagtatggc ttactacatt actcatgcgg aaggaaaaca tttccaaccg atgaatgcca
    18661 actatggtct gtttcctgaa ctgccagagc gcattcgaga caaaaaagca agatatgaag
    18721 caattgcaca acgagcttta aaagctaatg aggcaataaa aacagaatta gaaaaaactg
    18781 caatcccaaa tgagtaagga aactccatga agagggtgag acggatagtc tcgctctttt
    18841 tcttgcgttt ttcttaagaa tttctcaaac aaaatgtgag gaattgcgcc aattgtgaac
    18901 aaacttagaa atattggtta aattctgaca atttatttgt ctttttataa acgctgtgct
    18961 aaattattct ctgggacaaa aggaaggtga gaaaatgaca gaagccacgt gggaacaaaa
    19021 atttttgcgt tatttgattg tcgaaagagg atattcagaa aaaacgaaag aagcatatga
    19081 agaagacatt ctccatttca aacatttttt ggaggaatct ggagacgctg atctgctgaa
    19141 ggcggaatat tttacggttc gtgtctattt gagtgcatta gctgatcatc gtcctagcta
    19201 tagcagaaat tctatcagtc gcaagatagc cagcctacgt tcgttttatc agtatcttct
    19261 caaagaagaa gtgataaaag aaaatccatt ttcttacgtt catctcaaaa agaaaaattt
    19321 gagactgcca cgtttttttt atgaaaatga aatgcaagca ctttttgaca gcgtagcagg
    19381 agatagtcct ttggatttga ggaaccgcgc tttactggaa gtcctttacg ggtcaggcat
    19441 ccgtttaagt gagtgtagca gtttgtcggt atcagacatt gattttgatt cagaggtcat
    19501 gttgatccat ggtaaaggga acaaagagag atatgctcct ctaggttctt ttgctcaaga
    19561 tgctctgcaa gaatatttca gggaaggccg ccaggtattg atgacaaaat atcacgaaga
    19621 gcatgatatc gtttttgtga atcatcatgg aaaaccaatc acgcctactg gaattgaata
    19681 tgtcttgaat caaatgatca aaaaaagcag tttggatagt gatatccatc ctcacatgct
    19741 gcgccataca tttgctactc atttattaaa taatggggca gatttgcgga cagtccaaga
    19801 attgctagga catgccaatt tatcgactac ccagatctat gcccatgtaa caaaagaaag
    19861 tttacaaaaa aactatcgtt ctttccatcc aagagcgtaa aaataaggaa aagaacaaat
    19921 ttaggaggaa acaaacatgg tcgaatcacc atttcattca accactatct gtgccgtcga
    19981 aaaagacggg aaatttgcta tggctggaga tggccaagta acaatgggtg aatctgttgt
    20041 catgaaaggc actgcaaaga aagtacgacg tatctacaat ggagaagttg ttgtaggttt
    20101 cgccggaagt gttgcagatg cgtttacttt agaagaaaaa tttgaaggca aactaaacga
    20161 atataacggg aatctacaac gcgcagctgt cgaattagct caagaatggc ggacacagca
    20221 atctatgcaa aaacttgaag cgatgctgat cgtaatgaat gataaagaaa tgcttcttgt
    20281 ttcaggtaca ggagaagtta tcacaccaga tgatggtatc ttagctatcg gatcaggagg
    20341 gaattttgcg ttggctgcag cccgagcaat gaaaaactat gctcaaaacg acatgagtgc
    20401 gaaagaaatt gcagaaaatg cgttaaatat tgccgcagat atctgtatct ttactaatca
    20461 taatattatc gtagaagaac tgtaagaggt gagtagatat gtatgaatta aaacaaacac
    20521 caagacaaat cgtaagtgaa ttagatgaat atatcgtagg acagcacgca gcaaaaaaat
    20581 ctgttgccgt tgctttgcgt aaccgctatc gccgtttgca gttagatgaa aaaatgcaac
    20641 aagatattac accaaaaaat atgctgatga tcggacctac aggggtcggt aaaacagaaa
    20701 tcgcacgacg tttggcaaaa atcgtcaatg caccatttgt caaagtcgaa gcaacaaaat
    20761 ttactgaagt cggttatgtc ggaagagatg tagaatcaat ggttcgggat ttagtagaaa
    20821 atgcgatcca aatcgtagaa aaagagcagt acagccgtgt atacacaagt gctaaaaaaa
    20881 atgcagaaaa acgtttggta aaaatcttgg tacctggtat caaaaaagaa cagaaacaat
    20941 ccactaatca atttgaacaa atgatgaatc tttttaatgc tagtcagcaa aataccgctg
    21001 aaccgcaaga agaagtgaca gaagatattc gaatcaatcg ccgtacagtc ttggaacaac
    21061 tacaaaaggg tttactggat agtcgtgaag taaccattga agttgaagac caaaagaaac
    21121 ctgcaccaat gatgaacaat ggattggagc aaatggggat cgacctaaat gaaactctag
    21181 gtgctttgac accgaagaaa aaagtggaac ggacggtaac agtcaaagaa gctttagagt
    21241 tattgatcaa ggaagaatca gcaaaattag tcaaggatgc tgatatccat agtgaggcaa
    21301 tcaaattagc ggaagaaagt ggaattattt ttatcgatga aatcgataag atcacttcta
    21361 aatcacagca atctggagaa gtatccagag aaggcgtcca aagagacata ttgccaattg
    21421 tcgaaggttc acaagtaaac acaaaatacg ggatcatcca aacagatcac gtcctattca
    21481 ttgcttcagg agctttccat ttgtctaaac caagtgatct aattccagaa cttcaaggac
    21541 gcttcccgat acgggtagaa ttagatgatt tgacagctga agattttgtg aagattctga
    21601 ccgaaccaaa taatgcttta gtaaaacaat acgtcgcgtt gatcggtaca gaaaatgtgt
    21661 cagttacgtt tacaaaagaa gcaattgaac ggatcgctca aatcgctttc gatgtcaacc
    21721 gtgacacaga taatatcggc gcacgcagac tgcataccat tttggaaaaa ctactagaag
    21781 atctattatt tgaagcacca gatatgcaaa tgggagaaat aacgatcaca gaagcatatg
    21841 tggatgacaa acttggctca attgcggcaa atgaagattt aagtcgctat attttataaa
    21901 aaatcggagg acgacaatga cagacttgct aacgaaaacc cgcaagataa acacactttt
    21961 gcagcaaaag aatacttttg acatgcaagc agatttgcct tacgataaaa tggctgtcac
    22021 actaggtggt attcttgaca gcaacaccta tatcatagga agtgacggag tattactagg
    22081 ttataatgaa cgtcacgatg tgaacaatga acgaatcaaa acaatgtttg ttgagaaaca
    22141 atttcctgaa tcttatactg atacagtggc acagttgatg aaaactgaag ctaatatccc
    22201 aattactagt gatattactg catttccggt agagtcaaga gagaaatatc catttggtct
    22261 gacgactgtc gttccgatct ttggagctgg cgaacgtttg ggtacgatta tcttatctcg
    22321 tatggaacaa tcgtttgcag atgacgatct agtcttagca gaatatggag ctacggttgt
    22381 cggtatgcag atcctttatc agaagtctcg aagcatcgaa gcagatgtac ggagtgcaac
    22441 ggctgtccaa atggcaatca acacattatc ttatagcgaa ttgaaggctg tacaagctat
    22501 ctttgaagca ttggatggag atgaaggtcg tctgactgct tctaatattg ccgatagtat
    22561 cggcatcact cgttctgtga tcgtcaatgc tttacgaaaa ttggaatcag ctggaatcat
    22621 tgaatctcgt tcgttaggca tgaagggaac ttatttaaaa gtattgaatt cccgttttaa
    22681 agacgagtta gcaaaacaca gttattagaa gtaaggggac tggggcagat agaaatgttt
    22741 gttccagcct tcccatttta aaaagtaagt cgaagttgga ggaatggcaa tgactgttat
    22801 tattcaaaat gaccagctga tcgccgaaat cgctgaacac ggtgcggaat tgatcagtct
    22861 gaaatcaaaa gaacatggtt tagagtatat ctggcaaggt gatccagcgt attggggcag
    22921 acatgcaccg gttttattcc cttttgttgg acgtttaaaa gatgatcagt atgtttatca
    22981 agggaaaaca tatcaaatgg ggcaacacgg atttgctcgg gatatggatt ttgaagtcat
    23041 tgaacatgga caagaaatgg ctagtttcat cttaaggagt acgccggaga cattagaaaa
    23101 gtatccattt gattttgaat tagtgatcag ctatgaatta ggaggagacg gcatcacggt
    23161 tcattatcaa gtggcaaata ccggtgaaaa agaaatgttc ttttctatcg gtggacatcc
    23221 tgctttcaat gtacctcttg aagaaggatt gtcatttgaa gatttctatc tggcattttc
    23281 tccattgaaa tcaagattac gcctaccatt gaaaggccca ttcatcgata tggatcaaaa
    23341 gacgttaggt cagacaaata cgaatctggc tttgaatcgt gaactttttg agcaagatgc
    23401 cttggttttt gaaacaaggg gtctaaatgc gtacgccatt cgaagtgaaa aatcttccca
    23461 tagcgtaact ttaagctata aagatatgcc ttatgtaggg atctggtctc cttatccaaa
    23521 agaagcgcct ttcgtttgta tcgaaccgtg gtgcggaatc gctgacacgg tgaacagtag
    23581 tggaaacttg atggaaaaag tagggatcaa tcaactaggt gcccatgaaa tcttcaagac
    23641 aaaatattcc atcacagtga agtaagaggt tgtgaccgag aagttagcag ggaacaccgt
    23701 ttaccaaggt gtgtaaaaaa gctatcctgt aagtaataag ggagtgtgac atcgatttgt
    23761 cacactcccg ttttatctct ttggaatgaa aaagtatcag gattttttct tgctgctatt
    23821 caaaccaaac gggattcgac tttcagtatg gtttctgata cgcttgatat tgtctttatg
    23881 ccgataaaag ataaaggaag aaactaaaaa ggcaatgatc gtcagtaacc aattgggctt
    23941 agccaaaatc acaggagcta cataaggaag gacgatcgta gataaagtga tcaatactgc
    24001 actgatcata ctgctcaaac tcaccatact tgtacaaaat aaactaacga taaagatgat
    24061 acaagaataa acaaaaaata caggattaaa tcctaaaagc attcctgcac ttgtcgcaac
    24121 agcttttcct cctttgaacc cagcaaaaat cggaaatgta tggccaagaa tggcgcatac
    24181 cccaaaccaa agagggttaa tcccagaaag gtggaagatc atcggcaaac aagtagccaa
    24241 tgtacctttt aaaatatcca taagcaggac aacaatcccg gctgttttgc ctaatacacg
    24301 aaatgtattg gtcgttccag tattaccgct cccgtgttgt cggatatctt ttttgaaaaa
    24361 caatttgcca atccagacac cggaaggaat agaacctaaa agatatgcta aaataagtaa
    24421 taagatcaat ttcatttttt accctctttc aagttgaaaa ctatccttta ttttagcatg
    24481 aataggtaac aggaacaatt gaaaaaatga aagatacaag tataatagat ggaataaaaa
    24541 gaaaagcaag gggtggcaga aatgtctgta aaaaatccaa cacaagaaac gattttcaac
    24601 tatttggaag aagcaaaaaa tattgccgtc gtaggcttga gcaataaacc agaacgaaca
    24661 agttacatgg ttgcacaggt attgcaagac aatggatata aaatcatacc agtaaatcca
    24721 ttattggctg gtgaagaaat tctaggcgag aaagtctatg cgaatttgac agaaatcgat
    24781 gatccaatcg atatcgtaga tgtatttaga cggagtgaat ttttacctga aatagcaggt
    24841 gattttttga aaactgatgc taaagtgttc tgggcacaat tagggattga aaacaaagaa
    24901 gcagctgaga tgctaagaaa agcaggacgt acagatatcg taatggatcg ttgtattaag
    24961 attgaacttg gtaagttgaa agaaaaccaa aaataaaaat gaccacaaag aagagatgat
    25021 cgatagaggg gaatgtcgaa aaagcggtta ggagataatt aaaattgatt tctcgtctac
    25081 cctctttttt ttcacgacct tttctagtat gataatcgtg gtgcggtttt gccgcaaagt
    25141 attattgaag gagcgtttgc cttggcaaaa aaagtaaaca acgaatacaa tgactcgtcc
    25201 atccaagtat tggaaggatt agaggcagtt agaaaaagac cagggatgta cattggatcc
    25261 actgacagcc gaggcttgca tcatttagtc tatgaaatcg tcgataatgc agttgacgaa
    25321 gcattatcag gttatggcag tgagattgat gtgaccatcc atgaagacaa tagtataact
    25381 gttgcagata gcggacgtgg gatgcctgta ggaatgcatg cttccggaat accaacagtt
    25441 gaagtgattt ttactgtgct gcatgctggc ggaaagtttg gtcaaggagg gtacaaaact
    25501 tctggtggac tgcacggtgt aggagccagt gtggtaaatg ccttatcgaa atggctaatt
    25561 gtaacgatcg ttagagatgg cgtagagtat cagcagaaat ttaaaaacgg cggtaaacct
    25621 gacggtactt tgaaaaagat aggaaaaaca aaaaaagcaa acgggacgac tgtccatttt
    25681 ttaccagatg atacaatatt cagtacaacg aaattttcat atgaaatttt agccgaacgg
    25741 ttaagagaat ctgcctttct tctaaaaggc gttaaaatct ctctttctga tcttcgtgga
    25801 gaagaaccag tcaaggaaat ttttcattac gaagaaggaa tcaaagaatt tgttgattat
    25861 ctgaatgaag aaaaagatac attgacacca gttgtttatt tttcaggaga aaaagaaggt
    25921 atcgaagtag aggtcgctta tcaatacaat gacggttatt cagaaaatgt tttatctttt
    25981 gtcaataacg ttcggacaaa agatggggga acccatgaag ctggtatgaa agcggcgatg
    26041 acgaaatctt ataatgaata tgcgagaaaa gttggtttgc tgaaagaacg ggacaagaat
    26101 ctggaaggta gtgatttcag agaagggtta gctgctgtac tttctatccg tgtacctgaa
    26161 aatcttctgc aatttgaagg gcaaacaaaa gaaaaacttg gtacgccagt tgctcgtacc
    26221 gtagtagata acgtcatcag tgaacaaatg ggcttttatt tgcaggaaaa tagcgaaatg
    26281 agccagatgt tggtccgtaa agcaatcaaa gccagagaag cgagagaagc agcacgtaaa
    26341 gccagagaag aaagccgaaa tgggaagaag cgtaaaaaag gcgaatctct tctttcagga
    26401 aaattgacac ccgcacaatc ccgaaaccct aagaaaaatg aattgtattt agtcgaaggg
    26461 gattctgctg gcggatctgc taaacaagga agagacagaa agttccaagc aattctacct
    26521 ttacgaggaa aagtcatcaa taccgaaaaa gctaaaatgc aagatatctt gaaaaatgaa
    26581 gaaatcaata cgatgatcta tacgattggt gcaggagtcg gtcctgagtt ttctatcgaa
    26641 gactgtaatt acgacaaagt aatcatcatg accgatgcgg atacagacgg cgcccatatt
    26701 caagtattac tgcttacttt cttttatcgt tatatgaaac cgctgattga agctgggaaa
    26761 gtatatatcg cgttaccgcc tttatataaa gtatccaaag gacaaggaaa gaagcaagtc
    26821 attgaatatg cgtggacaga tgacgaattg gcagcaatga ttaaaaaagt cggcaaagga
    26881 tatatgctgc agcgatataa aggacttgga gagatgaatg cggaacaatt gtgggaaaca
    26941 acgatggatc cgacttcccg tacgttgatc cgcgtccgta tcgatgatgc tgcgcaagct
    27001 gaacgtcgag taaccacact gatgggtgac aaagtagaac ctcgaagaaa atggatcgaa
    27061 aatcacgtgc aatttacgct cgaagaagat ggcagtatct tagataaaaa agaggatact
    27121 gaaatctctc cttcagtttc taatgattta ttagatgaag aacgagcaga taaaaatgaa
    27181 aacaatcagt tatttgaagt agaataggag gtcacctgat ggaaaatcga caagaagtac
    27241 aagaattaac tttagaagaa gtgatgggtg accgcttcgg acgttattcc aaatatatta
    27301 tccaagaacg tgcactgcct gatatccgtg atggattgaa gccagtgcaa cgccggatat
    27361 tatttgccat gaacaaagat ggcaatacgt atgacaaagg attccgtaaa tcagcaaaat
    27421 ctgtcggaaa tatcatgggg aattatcacc ctcatggtga ccgcagtatt tatgaagcga
    27481 tggtccgcat gagccaagac tggaagttgc gggaagtact gatcgaaatg cacgggaata
    27541 acgggagtat ggacggtgat ccacctgctg cgatgcgtta tactgaagcc agattatcca
    27601 agttaagtgg agagatgcta gcagatatcg agaaagaaac agtcgatctc gtgtggaatt
    27661 ttgatgatac agaaaaagaa ccaacagtcc ttccagctcg atacccaaac cttctcgtca
    27721 atggatcgac aggtatatct gctggatatg caacagaaat cccgactcat aacttagctg
    27781 aagtgatcga tggcacaatc tatatgatcg atcatccaca agcgagcctt gaaaaattga
    27841 tggaatatat tcctggacct gactttccaa caggagggat cttgcaagga aaagatgaaa
    27901 tcaaaaaagc ttatgaaaca ggacgaggaa aagttattct tcgttcgaaa acaaagatag
    27961 aggcaatgaa gggtaataaa cagcaaatcg tgatcacaga aatcccatat gaagtaaata
    28021 aagcgacctt ggtgaaaaaa atggatgaga tccgtttgaa taagaaaatc gatggaatag
    28081 ctgaagtacg agatgaaagt gatagaacag gtctgcaaat tgtcgtcgaa ttgaaaaaag
    28141 acgccaatgc tcaaggaatt ttgaattatt tgttcaaaaa cactgagcta cagatcaatt
    28201 acaatttcaa tatggtcgct atcgaccata tgactccgca tcaagttgga ctgaaagaca
    28261 ttttaagaag ttatatcgag catcgaaagc aagtcataac aaaacgtagt caattcgatt
    28321 tagccaaagc tcaaaaaaga cagcatatcg tagaaggatt gatgaaagct ttatcgattc
    28381 tggatgaagt aatcgcaacg attcgtgaaa gtaaagacaa aaaagacgcc aaaaagaatt
    28441 tagtcgacgt ctttcaattt acagaagaac aagcagaagc aattgtcaca ttgcaattat
    28501 accgtttgac gaacacggat atcacagagc ttcaaaagga atccgaaagc ttgattgcgc
    28561 aaatcacaga gttgaataaa atcctttcaa atgacaaaga gcttttctct gttatgaaaa
    28621 aagaattgcg agaagtgaaa aaaaattatt cttccgctcg tctaacgaca atcgaagacg
    28681 aaatcgaaga aatcaaaatt gatacgcaag tcttagtggc acaagaagac gtaattgtca
    28741 gtgtgacgcg agaaggctat gtgaagcgaa caagccttcg ttcttatagt gcttccaaac
    28801 cagaagaaat cgggatgcga gaaggcgact acttgctata ctcaggtgaa ttgagtacat
    28861 tggaccatgt tcttctgatc acaaataaag caaatgtgat ctaccgccct gtccatgaac
    28921 tacccgattt gaaatggaaa gatgctggtg agcacatttc tcaaacaatc tccaatctgt
    28981 ctgtagacga atcgatttta gctgttttcc catatcaaaa aattcaagca gagaaaacat
    29041 ttgtattcat cagcaagaat ggattgatca agcaaacacg aatgacagag ttcgaaccat
    29101 ggcgcaccta taagagccgt ccgctcagct gtatgaagct aaaggcggaa gaagatgaac
    29161 tgattgctgt gtatctgaaa gaagatcagc caggcttaga tgttttctta gtaacgcatc
    29221 aaggaatggg tttgcgctat cctttaacag atgttcccgt tgttggagca aaggcagctg
    29281 gagtgaagtc tatgaatttg aaagatggag ataggattat caacgggctg cttgtgttag
    29341 cagaaggtga tacgccgatc gtaatcgtta ctcagcgagg agctgttaaa cgcatgcttg
    29401 ctcaagaaat cagccaaact tcacgtgcga agcggggagt gacggtcatg cgtgagctga
    29461 aaaaaaatcc gcaccgtatt atctatatgt cggaaggaag atcaaaagaa ataaccttga
    29521 tgaatcaaaa agggcagcag ttaacgattg acccaactga tttccctata ggtgatcgaa
    29581 catcgaatgg atcatttgcg atggatgaga aaaaaggtgg agaagtcatc gaagttatcg
    29641 atgctcctga gattttcatc gagtcgtaac tgttgtagta ttttaatgaa gagacgccat
    29701 ctgtattaat
//
LOCUS       DACDUU010000029        28674 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.029, whole genome shotgun sequence.
ACCESSION   DACDUU010000029 DACDUU010000000
VERSION     DACDUU010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.029"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            49..1190
                     /locus_tag="IWL30_001348"
                     /pseudo
     CDS             49..1190
                     /locus_tag="IWL30_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(1316..2395)
                     /locus_tag="IWL30_001349"
     CDS             complement(1316..2395)
                     /locus_tag="IWL30_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9706125.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(2498..2938)
                     /locus_tag="IWL30_001350"
     CDS             complement(2498..2938)
                     /locus_tag="IWL30_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9706126.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(2995..3405)
                     /locus_tag="IWL30_001351"
     CDS             complement(2995..3405)
                     /locus_tag="IWL30_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP9706127.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(3585..4019)
                     /locus_tag="IWL30_001352"
     CDS             complement(3585..4019)
                     /locus_tag="IWL30_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP9706128.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(4119..4967)
                     /locus_tag="IWL30_001353"
     CDS             complement(4119..4967)
                     /locus_tag="IWL30_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9706129.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            5356..7122
                     /locus_tag="IWL30_001354"
     CDS             5356..7122
                     /locus_tag="IWL30_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAP9706130.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            7224..8483
                     /locus_tag="IWL30_001355"
     CDS             7224..8483
                     /locus_tag="IWL30_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9706131.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            8630..9931
                     /locus_tag="IWL30_001356"
     CDS             8630..9931
                     /locus_tag="IWL30_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9706132.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            9934..10785
                     /locus_tag="IWL30_001357"
     CDS             9934..10785
                     /locus_tag="IWL30_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9706133.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            10798..11613
                     /locus_tag="IWL30_001358"
     CDS             10798..11613
                     /locus_tag="IWL30_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAP9706134.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            11764..12930
                     /locus_tag="IWL30_001359"
     CDS             11764..12930
                     /locus_tag="IWL30_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9706135.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            13160..13807
                     /locus_tag="IWL30_001360"
     CDS             13160..13807
                     /locus_tag="IWL30_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAP9706136.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            13891..14835
                     /locus_tag="IWL30_001361"
     CDS             13891..14835
                     /locus_tag="IWL30_001361"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAP9706137.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            14970..16421
                     /locus_tag="IWL30_001362"
     CDS             14970..16421
                     /locus_tag="IWL30_001362"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAP9706138.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            16442..17278
                     /locus_tag="IWL30_001363"
     CDS             16442..17278
                     /locus_tag="IWL30_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP9706139.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            17278..17937
                     /locus_tag="IWL30_001364"
     CDS             17278..17937
                     /locus_tag="IWL30_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9706140.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            18063..18752
                     /locus_tag="IWL30_001365"
     CDS             18063..18752
                     /locus_tag="IWL30_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706141.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            18758..19714
                     /locus_tag="IWL30_001366"
     CDS             18758..19714
                     /locus_tag="IWL30_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAP9706142.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            19758..20270
                     /locus_tag="IWL30_001367"
     CDS             19758..20270
                     /locus_tag="IWL30_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9706143.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(20526..21074)
                     /locus_tag="IWL30_001368"
     CDS             complement(20526..21074)
                     /locus_tag="IWL30_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9706144.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(21163..22344)
                     /locus_tag="IWL30_001369"
     CDS             complement(21163..22344)
                     /locus_tag="IWL30_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAP9706145.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(22580..24088)
                     /locus_tag="IWL30_001370"
     CDS             complement(22580..24088)
                     /locus_tag="IWL30_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAP9706146.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            24247..24438
                     /locus_tag="IWL30_001371"
     CDS             24247..24438
                     /locus_tag="IWL30_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706147.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            24641..25558
                     /locus_tag="IWL30_001372"
     CDS             24641..25558
                     /locus_tag="IWL30_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAP9706148.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            25551..26624
                     /locus_tag="IWL30_001373"
     CDS             25551..26624
                     /locus_tag="IWL30_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706149.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            26772..27290
                     /locus_tag="IWL30_001374"
     CDS             26772..27290
                     /locus_tag="IWL30_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706150.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            27271..27663
                     /locus_tag="IWL30_001375"
     CDS             27271..27663
                     /locus_tag="IWL30_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAP9706151.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            27739..28674
                     /locus_tag="IWL30_001376"
     CDS             27739..28674
                     /locus_tag="IWL30_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAP9706152.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
BASE COUNT         9621 a         5161 c         5564 g         8328 t
ORIGIN      
        1 ctactatctt atttcttgca tacaaaaaaa tcaaggagtg agagagttat gagatattca
       61 accatcttaa tatcaacatt tgcagcctta agcttatcgc ttactataaa tcagacagtc
      121 cacacagagg agacagaaac agccaaatgg acagaaacca agtgggccaa cgtcaatcga
      181 attacctttc aggatgaatt ttcatccttc aattcaggag tcgcaaaatt aagaaaagga
      241 caaacagcga ccatcaaagc aaaaattgac tattcgggag acttagcacc tatactacaa
      301 tcagctgtta catttgagaa tgttgatccc actttgtcga ttggctatga cgagaacact
      361 ttgactatcc aacacaagca agcgatattt accttcaccg ttacattaaa tcaggacttg
      421 ttaaaaccag cgctttttac tgtcaaggta tcagatggac taccgaacga tcatcattta
      481 acgccgtata gtcagcggca gacaattgag caagattcag ccatcgactc aggaggagat
      541 cttgttgaag aaccaacaga taaaccagaa aatgaaaata agccagaagt accgcctaca
      601 gaaaatccag acggagaaca gaaaccggaa atagaacctg gagaagaacc cgatacagaa
      661 actcaaccag agccagacaa taaatcaaaa cctgaaataa cccctggaga aaaaccagat
      721 gtaactccag aaccagatac ggattctgga aaccaaacag taccggaaac aaatccagac
      781 acagataatg aaactgaaaa tccagaaaaa ccagaggtag atccagaaga aaaaccagat
      841 gtgactccag aaccagatac ggacgcaaga gaccaaggaa taccagaaaa aataaacaaa
      901 aaaacaattc aagaggatgg aaaaaaagag agtaaaaaat caaatctagc aattctaaaa
      961 ataaatgaag agcagctgaa taaaaaatca cgaatatttg atagcgccca atcagctgaa
     1021 accttaaaaa gtagcaagga tacaaccttt gctgtcctga aacgaaaaat aaacagctac
     1081 caaaatccgg agagtcacaa aacaaagtta tactgtggag cggaatcatt ctattaagta
     1141 tagcaacaat gctttctgca aaaagattta aacaaaacag atctctctaa catttgaata
     1201 atgtctgtga acgcattttt caaataaaaa gaaatatcca gaaaatgacc ccaaaatacc
     1261 agattgaaat ctgatatttg gggtcgttca tactttatct ttcttcatat ttattttaag
     1321 ggatatctgt ttcttttgga agatggttcg ggatcttttt cactttcaat acatctaaaa
     1381 agaaggtaaa gatcggaatg ccgacaatca agccccaaac gccaaagaac cgttcgctaa
     1441 caagtaagac cacaaatgta taaaaaatcg gcagttctgt tttgctggac atcaacttcg
     1501 gattcagcac ataagactca attagatgga caatcaaaat cgtaatcagg atataaacaa
     1561 catctcggat gcctccttgg gaataagcta tgaaagatag aggaatacaa gaaatgataa
     1621 cacctgctac tgggatcaga cttaaaatga aaatcatgat cgctaaactc agcagctgat
     1681 gaaagccaaa tacagccaag gcgattgtag tgagcaacgt atttaaaaga gcgacaacaa
     1741 attgagcttc taataccaaa ccaaatgtat tgacaaactt atcggcaaaa tagtaaatat
     1801 cttgaaagaa ccaagaaaat tcacttttta aaaacaatct tgagaataca attgtttttt
     1861 tcttttcaat cataaaaaag aaacttaaga tgaatgacat agcaaacgcc actgccattt
     1921 ttcctatatc ctgtaaatag ccaagcgcaa tcgaagcgcc attttgaagc tgttcaaaaa
     1981 aatcgtttcg ctcaatataa gtatggatat attgtaaaac aggatctgca tcttttggct
     2041 gatgctcgta aaaattaata acggactgga tcatgtcaaa tgtttgatga ataagcatcg
     2101 ggatatactt tgtgaatgcc agatagataa ggtaaacgat cagtgtataa atcatgatag
     2161 ctaaaacacc tgtagggatt cggacaaatc gctgaatgat ttgaacaaga tgaatcatca
     2221 aataagtaaa gataaatgtg agcaaaatgg ttgtaatcat acttctaact aagaaaagaa
     2281 caaaaacgac aaataataaa acagtgagac ggcgtatttt ttcattcgcc aaaaaacgct
     2341 gataaacggt caaatcgaca acctccaaat aacaaaaaat aaacacgtta ttcattatat
     2401 gatggaatat gaaaataaga aagagcaaaa agaaaaaccg ctaaatgacg ttatcttatg
     2461 agttggtttt cctcataaaa tcgttcatct agcagtttca gccctttttc tggtcttgat
     2521 gaaaattatc tagcattttt cgggtcagat gttgcagtgt atccaattct tcctgttcaa
     2581 gtggaagata agacttgatc ttttgtggaa taccttctgc ttgtttttgc aacgcagcac
     2641 cttgagcagt cagcgtaaca tttacgattc tttcatcacc cgtttcacgc tgacgattaa
     2701 tataattttt ttctgctaac ttttttagta agggagtcag cgtaccagaa tccagacaca
     2761 aatcatttcc tagctctttc actgaagtct ttttcttttc ccataaaact aacatcacaa
     2821 gatactgggt ataggtaaga ttcagcggct tgaggagggg agtgtagtgt ctaatgattt
     2881 ctttagaggc ggcatataaa ggaaaacata cttgattaac taaatataga tcttccattt
     2941 ttccacctcc gctgttttac tatagtgtat catagcggat ttacacaaaa aagtctattc
     3001 actaactgct ttcagagtaa cttctatatt tccagctgta gcttttgaat acggacacac
     3061 tttatgcgct gcctctaata attcttgcgt ttcttccaga gaaagacctt cgatagatcc
     3121 ttcaatctct acacctaaga caaagctcgt ttcactttct gaataaaggg aaacttgtgc
     3181 actgacagta gaagccccag agatattttt ttgctgtttg atcaattcta atgcactgtt
     3241 aaaacatgca ctgaaaccag ctgcaaacaa ttgttcaggg ttggttgcat tttcgacgcg
     3301 tttaccagga gctgcaatat tcaattcgaa tgtacgatcg ggattatgca cttctccaga
     3361 gcgtccgcct gtgttgatca ttttcgtaga atacattttt ttcataaaat taccaccttt
     3421 aattatattt tatacaattg aattttacac aatataattg tggaaagcaa agaaatggat
     3481 tggcataaga taaataagag aaagcacatg aaacagacgc gctttctcga atagcaaaca
     3541 tatacaaaaa atatcagaga caaaaaataa tctcatcata aacattacat atatttctca
     3601 aaaagttgtt ttctttcttc aaaaattact tcacgatgga cctgatgaag catccataaa
     3661 taaccaaaag gatctctgaa cattgagtta gtcacaccaa agtccatcat ttctgtcatt
     3721 tcttgaatct ctacacagcc atttcgtaat gctttattat gtgtgtcctc gatatcagag
     3781 acgatgatat tgaaccagat cgtatttgga tgttcagcag taggagcagt caaaccaaga
     3841 gacgtgtttt catccagcat atggaatctc gtaccataca aggtaaaaat gacttcatta
     3901 ttgcctctag ggtaatctgt tgcctcaatc agttgtattt catcaaagat tgtttgatac
     3961 agttctagtg ctttcaaact atcatttaat acaaaatcga tttccgttcc tttatgcata
     4021 aagatctcct ctccttttca gtcgaacagt tactagattc ttttattata ccagctaaag
     4081 aaaacgcatt caaaaaaata gcggatttac ttaccaactc attcgattgg atgataagta
     4141 aatccgcgtc ccattacttc tgctacgctg ctgacaatga caaaggcact tggatcgatt
     4201 tcttctatca atggtttcat tcgggaaaat tcatgatcag tcacaacgat caataaaacc
     4261 tcttttgact ttcgattata cccgcctttt gcctctagca gggtaaggcc acgtccaatc
     4321 tcagcactta agcgaaggcg gatgtcttcc aaataatgtt cactcatgac cataacagat
     4381 ttctttcgat tgatacctgt ttcgatataa gtcatgacaa ttgaagtgat tgcaatagat
     4441 aaaatagcat aaagaaactc ttctattcca aaaataaaca gattaagaga aacaacgaat
     4501 gcgtctgtaa aaaataagcc aatcgaagta ttcaagtgga aatatttttt aaaaatgagg
     4561 ggagggatcg tcgtaccgcc gctagaagag ttgtttttat ataaaatcgc aactcctaaa
     4621 gcaaaaatcg cacttccaaa aatgacagac aacaaccgat ctgaagtaat cattgtttct
     4681 ggaactattg caatgattaa aggaaaaacc aagcttccga aaagtacttt aaaaaagggt
     4741 tttttaccta aaaatagaag agctagtacc aacataacga tattcaacac taaaataact
     4801 atagctcggt caaagcctaa agcagcttcc agtaatattc ctagaccgct gacacctcca
     4861 gcagcaatat gatgaggccc taaaaacata ttgatagcga ctcctaatat gaagagggca
     4921 aaaataatct taatgtatgt ccatataaat tgtctcgttt ctttcattta ttcacttcct
     4981 taattcatta ataatactct tatcttatta taacttactt ttcaccttaa gttaacttta
     5041 attaataaaa aaattaggct agtctaaatt attatgtttc attcttctag aatatacttc
     5101 tacgccaaaa actgttaaaa aacttttact ttagaaaatt ggagaaattg atcgcttaag
     5161 ataaattcag gacactaaat agtacaggaa gtagactaaa acttttaaaa gttttaagtc
     5221 tatctatttg gcaagtttaa aaaataaaaa ttcggtatca aaaaactgtt accgataaca
     5281 tttttgaaag cgttgtcttt tgcttctcta tcacttatag tgatgacgtc atattaaaac
     5341 acaaggagga aatcaatgga tacagctgca atttatcatc gaccggaaag tgaatatgca
     5401 tttctatata cgaatacaag atttcgtatt cggcttcgta ctaggaaaaa tgatatcaaa
     5461 aaagtatatg tgctatgtgg agatccatat accataacaa ctgaaaaatg gtatcaaaaa
     5521 caacgaccaa tgaaaaaatg tctaagtacc aatgttcatg attattggga aattgaagtg
     5581 acgagcgaaa caagtcgctt acagtatgcg ttccatgttg ttggggaaga taacacggac
     5641 tgtttttacg gtgatcaagg aatttttcct tatcgagaaa atgtgattac tgaaccaaat
     5701 ttttattttc gaatacctta ttttcatcaa atcgatcgat tcactattcc agaatgggta
     5761 aaagaaacag tatggtatca aatttttccc gaaagatttg caaacgggga caaagcaaat
     5821 gaccccgaac acactttacc ttggggaagt aaagatccag accgagaaga ctttttcggg
     5881 ggagatttgc aaggtgtgat ggatcatttg gattacttga cggaccttgg catcaatggc
     5941 atctattttt gtcccatatt taaagccaca tccaatcata aatacgatac gattgattat
     6001 tatgaggtgg atcccgcttt tggcgataaa aaacttttaa aaaacttgat tgatgaagca
     6061 cataaaagag ggatacgtat catgttcgat gctgtattca atcatatggg tgtccattca
     6121 ccacagtggc aagacgttct caaaaacgga gaaaaatcga tctataaaga ctggttccac
     6181 atccgttttt tcccagtaga ttcttatcag atgactgact taccagaaac tgctgaaaat
     6241 atcccatatg atacttttgc ttttactcca ttcatgccga aactaaatac agcaaatcca
     6301 gaagtgcaaa aatatttgct ggatatcgca acttattgga taaaagaatt cgatattgat
     6361 ggatggcgtc ttgatgttgc taatgaagtc gatcatcatt tttggaaaaa attcagagaa
     6421 gcagttacag aaatcaaacc agacatctat atccttggag agatttggca ttcctctcaa
     6481 gcttggctgc aaggggatga attccatgca gtgatgaatt atgcatttac agattcgatt
     6541 aaagattatt ttgcaaaaaa gaaaatcaca gcttcacaaa tggtcagtgg aatgaatcat
     6601 cagcaaatgc tttacagaga tcaggtgaac gaaggaacat tcaacttatt agattctcat
     6661 gatactgcca ggatcttgac cctttgccaa ggtaacaaag aattgatgaa gtctgtgatg
     6721 gcctttatgt ttcttcaaaa agggtctcct tgtatctatt acgggacaga aatagggatg
     6781 actggtgaag atgacccgga ttgccgcaaa tgtatgatat gggagaagga ggaacaagat
     6841 ctagagctat tcggttttat aaaagaactt gtttctctaa ggaaacagtt gtctaagatt
     6901 atttctgaag gttctactca gtggctaata gtaaatgatc gtgaagataa gctctatttc
     6961 acacggaaac tagagggaca gatcatctat gtttacttta atcagtcaaa agaaccttgg
     7021 gtagtagaac aggaaaatga ggtgattctt agtcaaaatt gtcagttgtt agaagacggg
     7081 aaagcagaga tacgacaata cggatttttg atcatgtgtt gaaaactgtt accggtaaca
     7141 acaagaaagc gtttgcaaat gaagatttgt gcccattata ataagggtgt aacagcacgt
     7201 tgaatacaaa ggaggaaatg aagatgaaaa aaagaagtgt gaaacagttg ggtttagggt
     7261 taatctcttt aggaatctta ggcggattag cggcttgtgg caatggtaac tcttcagcag
     7321 aaggggaagc cgatgaagga aaaacgttaa cagtttccgt tgattctgga tataaagatt
     7381 atataaacga aataaaaaac actttcgaaa aagaaaacga tgtgaaaatc aagctagtag
     7441 aaaaggacat gttcgatcag ttggaatcat tagcgttaga tgggcctgct ggaaagggac
     7501 ctgacgttat gatggcagca tatgaccgga tcggtgcgtt aggacagcaa ggacatctgg
     7561 cggaagtaaa actgggaaat gaagcagctt acgatgaaac agacaaagcc caagtgactt
     7621 atgatggaaa aatctacgga gaacctgcag taatcgagac ccttgttttg tactataaca
     7681 aagatttagt cgatacagca ccatcaacgt ttaaagattt ggagaatcta tccaaagaca
     7741 gtcgttttgc ttttgagtca gaagcaggaa aaaatacagg atttttagct aaatggactg
     7801 acttttatta ttcctatggt ttaattgcag gttacggtgg ctatgtattt ggtgatgatg
     7861 gcacaaatcc ttcagatatc ggtttgaata atgcaggtgc tgtagaagga atctcttatg
     7921 caacagattg gttcaaaaat gtttggccaa agggaatgca agacataaaa agtgcaggag
     7981 acttcgccag ccagcaattc atgtccaata aaactgcagc aatcattgac ggtccatggc
     8041 aagcgcaaac atacaaagaa aacaatatca attatggtgt agccaaaatt cctactctaa
     8101 ataatggaca gccttatcaa ccgtttggcg gaggaaaagg atgggtagtc agcaattatg
     8161 ctaaaaacaa agacctctct caaaaatggc ttgattatgt aacgaatcag gaaaatcaag
     8221 aaaaattctt tgaaatggta aatgaaatcc ctgcaaacca acaagctagg gaaacagcaa
     8281 aggctaaaaa cgatgaatta acaacagctg tgattgaaca atatgaaaca gctcaagcga
     8341 tgccaaacat cccagaaatg ggcgaagttt ggacaggtgc agaaaacttg atgttcgatg
     8401 cagcatcagg ttcaaaaaca ccaaaagaat ccgcagatga agcagtaaaa acaatcagtg
     8461 aagccataga acaaaaatac tgattaaaat gataaccgtc catttttata aaaaatggaa
     8521 caggtgcttc agataaaagt ggcaagagcg agatggaaaa ttatcttgct cttgttgcca
     8581 ttatcttaaa agcagtttgc ggtttagcaa gtttagaggg ggcattcaga tgcagcaaga
     8641 taaaacaagc aatatcaacg aaaaaaaaat cacgctttgt tcacttattc cagggatggg
     8701 gcagtttgta aatggccaag cttttaaagg tatcgttttt ttcgccatat tcgtcctatt
     8761 cattggtcag atgcttctgg gcggatttca tgcattggta ggattggtta ctttgggaag
     8821 tataccaatg gaagatcatt cattatttat tttgattgaa ggcactttgc aattcatcat
     8881 tacaatcatt ttcttgattt tttacggttt gaatctttta gacgcttatc gagtagcgaa
     8941 gatgcgtcaa gagggcaaaa aaatcaatcg gacgatgaag gaagtcatac ataatatagg
     9001 tgatgaaggt tttccttatc tattgacact tcccgcttat cttctcatga tttttacgat
     9061 cattttccca gtcctagtta cattgttcac tgcatttact aattatgact tcaatcatat
     9121 tccaccagcc agtttaattg attgggtagg ggctaaaaac ttttttagta tcttcttttt
     9181 aagctcatat cgtaatactt tttttgcagt cttttcgtgg accgtgattt ggactttatg
     9241 tgcaacaact ttgcaaatca cccttggtat tctcacagcg gttgttgctc accagaaatt
     9301 tattaaattc aaacgtattt tcggtatcat tttcttatta ccatgggcgg tcccagcttt
     9361 tattactatc atgagttttt cgaatatatt caacgatagc gtaggtgcca tcaatactca
     9421 agtgattcct ttcataaacc agcttccttt tgtagatatc agtgcaatct cttggaaaac
     9481 ggacccgaac tggacgaaag tagcaattat cttagtgcag ggctggttag gatttcccta
     9541 tatctatgtg atggttacag gaatcttaca atcgatttca gaagacttat atgaagcagc
     9601 aaaaatcgac ggagcaaatg catggcagcg cttttcaaac atcacgcttc ctgctgtctt
     9661 cctagtggca gcgccaacat ttattacaca gtatacgggg aatttcaata atttctccat
     9721 gatctatctg tttaacaatg gcggtcccgg aagcgtaggc ggaggagctg gttctactga
     9781 tattcttatt tcctggatct ataaacttac gactggaact tctccgcagt attctatggc
     9841 ttcagcaatc acgttgatta tctccgtcat cgttatctgt gtgtcattgc tggtatttaa
     9901 aaagacaaat gcgttcaata tgggggatta acaatgaaaa aatttcattc gagtgctcga
     9961 ttccaaaaac gactttctca cttttttact tacttttttc ttttaatact gtcagtcatt
    10021 atcatctatc cgcttttgat cacagcaagt actgccttca agtcaggaaa tattgcagct
    10081 tttagtttag atctttcaag tgactggact ttaggaaact ttactcggct attcagtgaa
    10141 acactttacg ggacttggta taaaaataca ttgatcattt ctgtcctaac aatgatcgta
    10201 caagtgactg tagtaacttt ggcgggctat acttatagtc gctatcgttt tatcggccgc
    10261 aaatcaagtt tgatgttctt tttagtgatt cagatggtac cgactatggc agcattgact
    10321 gccttttatg tcatggcttt cttattagaa gcgctcgata agtactggtt cttgacaatg
    10381 atctatatag gcggtggaat tccaatgaat acttggctga tgaaaggata ctttgacact
    10441 gtaccaatag atttagatga atctgctaaa cttgatgggg cagggcattt caggattttt
    10501 ttccagattg tattaccgct tgtcaaaccg atgctcgccg ttcaagcatt atgggctttt
    10561 atgggacctt ttggggattt catgttagct aagttcctgc ttcgaacacc tgaaaatcag
    10621 actgttgctg tcggcttaca aacatttatt tctgatgtga caaaccaaag agtctctctt
    10681 tttgctgctg gggcaatttt gattgcacta ccgatttgtc tattattctt ctttttacaa
    10741 aagaactttg ttaccggcct gacatctggc ggaacaaaag gataggagca gagtatcatg
    10801 caaacatttc cgttaaacta tttttccgca ttacgtacgc caactcaagt gtttgcggga
    10861 cgcaaactat tatcatggcc taaatttttc ttgatatttg tatttttagt ttccttgatg
    10921 gtcatgcctg tgactctttt ttatgccaat cagatccaag cgattccatt agaacaattt
    10981 ctatccgttc attcgctgat cgatgaacaa ggaacccaaa aattttctga acttgaatta
    11041 tctgaaactg ggttacaagc atcgcaacaa acgatagctg tgacccctga aattttggta
    11101 ggtgtgtctt tatctgagaa acaacaatcc gatcatggaa cttttattga ttttgagaag
    11161 gaacaatggg tgatccaaca aaaggataaa tctggtatac gccgctatac gatgaattat
    11221 agcccatctt tccaaccaga ttctgtacgt acgcccgaag atttccaacg atttttagaa
    11281 agagaatttt atgccagtaa tcgtccaaca atcattttat cttatagttt aagtcttggt
    11341 cttgtcctat ttgtcatgac gagtctcatt ttattcgggg cttccttttt cttgtggatg
    11401 acaagaaaaa gccaactttc atccattcat acattcaaag agagtgcaaa tttgatgctg
    11461 aatgttatgg gaataggttc catggcagca gcagttgtag ggtttatcca ctttgacttt
    11521 atcttgatgc taagtgtcca aacaacgata actgtcttgc tgcttttatg ggtttttgtc
    11581 aaaacaagat tcaaagataa gcgtgtcgaa tgacaagttt aagcgattta tgcgacaatg
    11641 aatgtgaaaa aaatgaaaaa taaagtagtt tttgaaaaat ttctgaaaaa attaaacttc
    11701 ttagttgatt cttttctttt ttagtattaa cgtaataggg tagttttttt aaggaggaaa
    11761 atcatgttag agaaaacttt atacaatcaa ttattttcac gttctttctc gctccctgtc
    11821 gaagtaaatt attgggacgg tacaacgaaa caatacggag atacggacac tcctccccaa
    11881 atcaaaatca atatccatga agaaatccca atgaaagaga ttacaagtaa cgcatctttg
    11941 gcactaggag aagcctatat ggatcatcga atcgagatcg agggaagtat ccaagcattg
    12001 atcaacgatg cttatcagca agcaaacagc tttttacgag gaaaagatta tctaaaatgg
    12061 ctgccaaaga aagaaaaaca tacgaaacaa aaaaacaaag aagatatcca tgcgcattat
    12121 gacttaggta acgactttta taaactttgg ctagatccaa ctttgactta ttcttgtgct
    12181 tatttcaata cacctgaaga tacgttggaa caagcacaaa tcaataaagt ccatcatatc
    12241 ttagacaaac tctttatcaa agaaggcgaa acgttgcttg atatcggttg tggctgggga
    12301 acattgatgt tcactgctac caaagagtat aatgtcaagg caactgggat cacactaagt
    12361 gaagaacaat acgatttgat taccaaaaga ataaaagaag aacatttaga agataaatgc
    12421 cgtgtattat taatggatta ccgcgaacta aaaggcgaaa cgtttgatca tatcacaagt
    12481 gtaggtatgt tcgaacatgt aggttctgaa aatcttgaag gttatttcaa agtggtaaaa
    12541 gatttattga aaccaaaagg aacagctttg atccatggaa tcagccgaca acaaggcggt
    12601 gcgacaaatg cttggatcaa taaatatatt ttccctggcg gttatgtccc tggtgttact
    12661 gaattagttg ataatattac aaaaaacgat ttgcaactga ttgatttgga aagcttacgt
    12721 cgtgactacc aattgacatt agaacattgg acaaagaatt ttcatcaagt ccaagatcaa
    12781 gtcacgaaag aaatgggtga ttccttctat cgaatgtggg atctatattt acaagcttgt
    12841 gcagcttcat ttgaatcaag taatatcgat gtgattcaat atttgcttgt tcaacctgga
    12901 aacaatgaca tcccaatgca tcgtgcataa ttgaggcaaa agtactgttt aaagactatt
    12961 taaaaagaag ctaagtttta tgatgctcca gaagtgaact tttttgtcta acttctagga
    13021 ttgtctctaa agcttagttt ctttttcttt atattttcat aataaaagtt tggttcttta
    13081 atttttaaac atgacagaaa attttttctt cgttatactt gtaatagaat actattttat
    13141 aacaaagggg atcgaatcaa tgaaaaaaac tgttatttat actagtttat ttgcagcttt
    13201 gtttttagct gcttgtcaat caaataccaa tcaaggcaca tctgggaata cgtcttccag
    13261 tagcgaacag cgctcttcac aggtatcgga ctcctcaaca aaagcaagca catcttcgtc
    13321 tagccagaca aaaagttcta gtgaagcaag tactcagtca gatatcaaag tgtctgcagc
    13381 tcgtgcgatc gaattgttcc aagataaata cccagatact gcaattacta gtttggaatt
    13441 agatagtgat tggggatctt acttctacaa gatagaaggt gtcgatgatc aaaaagaata
    13501 cgaagcaaga attgatgctc aaaatgaaaa aatggagaca gagaatccag aaactctaga
    13561 tcgagatgaa caaaatggtc aaaagaaaaa agaagatggt ctggatgttg ataaattgat
    13621 cacgattgag gaagcagcaa aaatcgccgt cgacaaagta ggaagcggta cggcaacaga
    13681 ttgggatttg gataaagatt taggtactac ttattgggaa gtcaaagtaa aagacggcaa
    13741 tcaaagtaca tcagtcaaga tcaatagcca aacaggcgaa gtactgtcaa ctgaacaaga
    13801 tgattaagaa tttcttcgga aaacaagagt tgatttttct tttagtcatg atacactaga
    13861 caaaataact tgtttgaagg agcgatttac ttgagtttag aaacgacctt tgccaatcat
    13921 atctttgcca atcctttgat gaatgcatct ggtgtccact gcatgactac ccaggaatta
    13981 gatgaactgg ctcattcaga agcaggagca tttatcacta agagctgtac gatcaatgaa
    14041 cgaaaaggaa atcccgaacc acgctatttt gatgttcctt tagggagtat caattctatg
    14101 ggacttccaa acttaggttt ttcttattat ctagagtatg ctcttgcgta tgaaaaagtg
    14161 caggaaaacc aaaatcaacc gttatttttt tccattgccg ggatgagtgt ccaggaaaac
    14221 ttggaaatgc ttgagaaaat agaaaaaagt gggtttaatg ggatcactga attaaattta
    14281 tcttgtccca atgtgcctgg aaaaccacaa ctagcttacg attttgaggc cacttatgaa
    14341 acactgaaag aagtattcag catcttttct aagccgctag gaatcaaatt gcctccttat
    14401 tttgattttg ctcattttga tcagatggct gatatcttga atcagtttcc attgacctat
    14461 gtcaatgcta tcaacagtgt gggcaatggt ttgtatatcg atacagaaca agaagcagta
    14521 gtcatcaaac caaaagaagg gttcggcggc attggcggag agtatatcaa gccgactgct
    14581 ttggcgaatg ttcgcgcttt ttatacacgt ttgaaaccag aaatccaaat cattggtaca
    14641 ggtgggattc ggacaggaca agatgcattt gaacatctgc tatgtggcgc aagcatgtta
    14701 cagattggta ccgagcttca taaagaaggg ccggaaattt tttctagaat cataaaagaa
    14761 ttaacgcaga ttatgtcaga aaaaggatat acgtcaattg acgaatttaa aggcaaatta
    14821 cgcaccattt catagcaaag agacgaaggt catgattgat cttcgtcttt ttttataaaa
    14881 aacactcgtc tgttcattcc tgtgcaagcg tactcattgt atgttagacg aacttgtggg
    14941 aaaataacag aggcaaaaag gaggataata tgaatgatgc aacagaatta gcaaaagagc
    15001 ttaaagaaaa agaatttagt tttcaagaac tagttacgga tatacagaaa aaaataaaga
    15061 agcaaaatca tgaattgaat gcgtttgtcg cgcttgaacc attcgagagt aattcccacg
    15121 agctattgat tccccagtca gaaaaagggc cattggctgg tatcccattt cctttaaaaa
    15181 tgctcggtca agaaaaaaag ggatggctag ctacttcagg atctcgttta tttgaaacac
    15241 accgagcttc tcatacatct aattatgtgc ggcaagcaga agcgataggt ctagttcctt
    15301 ttggacaaac caatgcgcca gagtttggtt tcaaaaatat tacagaccca gtcatctatg
    15361 gcccagcaag aaacccttgg aaccttgaat gtacaccggg cggatcaagt ggaggagctg
    15421 ctgctgcagt ttcatcagga atcgttcctt tagctggtgc tagtgatggc ggtggttcta
    15481 ttagaatacc cgcttcgttt tgcggattga ttgggctgaa accttcgcga gggacgatgc
    15541 cagttggtcc atatgcttgg agaggatggc aaggagctgc tattgatttc ggcttgacag
    15601 tttctatgag agacacggag gctttatttt atgggatgag aagtatccat tccggtgcgc
    15661 catatcaagt atcgccagtt gagtggcaaa cacatccaag aaaaaaacgg ttgaagatag
    15721 ctttttgtgt tgattcaccg atccactcac agattagtga agaggcagta cttgctgtaa
    15781 aaaaagctgt agatttcctt ggaaaagaag ggcatgattt gacagagatt ccttacccac
    15841 tagatggtcg taaactgatc acgtcttatt atcagatgaa tggcgcagaa acagcagcaa
    15901 tgatccgctc gattgaaaca ggcatccagc gagctgtcag aaaagaagaa cttgaaccca
    15961 tttcttggac tttgttccaa tatggtcaga agctctctgc cgctacttac atcgattctc
    16021 tgcatgtatg ggatgaagca gcaattgtca tggaaaacct acatcaagaa tacgatctat
    16081 tcctttcacc aacgactgca tttaccgccc ctaaaatcag cgaagatttg caaagtgatg
    16141 cgataagagc acagatggaa cacatcagtg attgttctga agtagaaggt ttagacgttg
    16201 tttcttccat gtttgaggag agcttgaaga tcacacctta tacacagttg gctaatctga
    16261 ctggacaacc agcaatcagc ttgcctactc atttgacaaa agaaaacatg cctctaggaa
    16321 tccaatttat ggctgccaga ggaagagaag atttgctatt tcaaatcggc tatctatttg
    16381 aagcaaatca tcagttccat ttaccaaatc catggaaata gcagaaacaa ggagaagaag
    16441 aatgaagaaa aaaagattgt ttgctctaat aactagtgcc ttttttattt taagcgcttg
    16501 cggtacagct gccgctaatc aggaagataa cagctggagt aatgtagaaa aaaagaaaga
    16561 attagtagta gcgacttctg gcacactgtt tcccgcatct tattataatg atcaaaatga
    16621 attagttggt tatgatgtag atttggcaaa agaggtggct aaacggttaa atgtcaaaat
    16681 ctcctttaaa gaatacaacg tggatggaca aatcacttct gtttcaaaaa gagaatcaga
    16741 ttttgctgcg aacgattttg gtatcacaaa agaaagagaa aaaaaattct ccctttcaga
    16801 gactttcaaa tattcatttg acagtatgat cgtacgaaaa aaagatgatt caggaatcca
    16861 ctcacttgaa gatcttaaag ggaaaaaagc agctggcgaa cccaatacta gctatatgcg
    16921 tttagcggaa aaacacggag ctaaattagt cacatatgac aatgccacga atgatcagta
    16981 tttaagcgat gtagcaaatg gtcgaacaga tgttattcta aatgattatt atctgcagaa
    17041 aatggccgta tctgctttac cagatatccc tgtaaaaata ctggagaacg tttatttcaa
    17101 tgaaaacgaa agtggttttt tattgaaaga aggaaatgat gctctgaaaa agaaaatcga
    17161 tggcgtatta aaagagatga aagaagatgg gacgacaaag aaaatatctg aaaaatattt
    17221 tcaaactgat gtttcagtca aaccgaaaga aacgatggct gagtcggtga gtgagtgatg
    17281 tactttcctc taattgattt ttccctcatg tggacttcac ttcctttcgt actgcaaggc
    17341 ttggtataca cattagggat tggcttcgtg tcgtttcttt taggaaatct ggtagggttg
    17401 cttttgacag ttttaggtct tttggattgg ctgcctctga atgtatttat tcgtttttat
    17461 ttatcgttct ttagaggcat tcccgcacta gtcctgctgt ttctgttata ttttgggtta
    17521 ccttatcaat taagtgcctt aacggcttca gtcatctgct ttacgattac cagcagtgca
    17581 tttattggtg aaatctatcg aggatcacta gcaggcgttt cttcaggtca gtgggacgct
    17641 ggtttagctt ctggtttttc tttctttggg gtattgaaat atatcgtttt gccgcaagct
    17701 tttcgaatct cagtaccggc actcagtaac gtagctatgg atctagtgaa aggtacttcg
    17761 ttagcagcaa caatcacagt gccagaaata tttcaaaaag caaagatcat aggcggtcgg
    17821 acattcgatt atttttctct ctatatttta gttgcactga tttattgggt catttgtatc
    17881 gggatcgaaa aagtgcaaca gtatttagaa aaacgttcta tgcatcagtc aatctgaaaa
    17941 atagaaatgg tgctagcgtg gctggcacca tttctatttt ttgtttcttg tatattctgc
    18001 ctagtatttt aaaaccattt catgtaaaat gtaaatcgaa aatatgttcg gaggtagaaa
    18061 acatgaagac cccgctaacc atcgaaatag aatcctcgtt ataccaatat tgcatcgagc
    18121 aaggcggctt ggttgtagaa gaagtcacga tgcctgatga ccaaggaatt gtagatacgc
    18181 ttagctgctt gacaaaattt gatggaagcc gagaatggcg atgctatgag ctgaaagtga
    18241 caaaagcaga ttttcgctct acagccaaac tgtcatttgt tggacattat aattattttg
    18301 ttttaccaag taaactatat gaagaagtaa aaactgaaat ccctacagga ataggtgttt
    18361 tagtgtatcg tccgtacacg gttgaagagg agatgcctgc ctctggtaca tttctgattg
    18421 cgaaaaaagc agcaaagaaa gagcttgctg taccggaaga agctttgaca aaccgcttca
    18481 tggcttcttt attcagggaa gtaagaaaag caaagcaaat ggagcgaggc gtaaaatatt
    18541 ttccaacaga tcaattgtac aaagaattga aaagacgttc agaaaatcaa gatccatttt
    18601 ctaacgaacg atattatgaa cggtttttag aggaaatagc tagtcaggca atcaatgagt
    18661 tgcaagaaga agttatcgcg cttcagcatg attatgaata tttgaggcaa caacagcaga
    18721 tccgtcggtt gcctacagag ccattggagt gaaaaatatg tactatcctt atttaagagg
    18781 aaaacaattt gacctattag cattaaaaga agcactgtcg cgaggattgc tctccaataa
    18841 aatccaacca gtgatcgaac cagtacgtga ttccgctacg cttaaaaatg tgatcgaatt
    18901 atttcaaaaa aagcaccatc gaatcgctgt gatccaaaat ccccaagtag gacaattcaa
    18961 attattcgat cagcatgtgc acagttggac agtcaaagaa aatagctctg tcgtccctgc
    19021 acagatctta acgccagaaa atctcagtga agtactggat tcacctccag cgtttcttgt
    19081 tttcgatgga cagcactacc caaaagatac agaggtgtgg aaacagttgg caggagttga
    19141 ttcgaaattt ttgataccag atactagtcg ctttcgcatc tggctgcccg aaaacaaaat
    19201 tgtgatccgt gactcttttc aaacgagaaa gcatgttgaa agttatgcag ataaaacaga
    19261 tgactttttt agtgatgact atcttttttt ccacgcagac ggatatattg gattttctga
    19321 tttcacgatc gaaggtagcc gttatttcga taaagggttc cctagccgag cgcttgctat
    19381 ccatctaacg tatattgatg cttacggcaa tatacgcgtg aaacattttg tctctgacag
    19441 taacgactct gctaaagacc aagcattgaa gtttcttgaa gcaggagata aaatgaaaaa
    19501 atggatcatg cgtcaccatc atcaactctt gattacttca ggaatgattg aattattggc
    19561 tctttaccag caacaaaaat tccctggact gggagtgttg aaaaagtggt cacttatgca
    19621 tcatttggaa cttattagtc acctgctaga tcatccaaaa gattggctta aatattattc
    19681 aaaagttcag gaactatatg aagtgatcca atgattagat aatgtaaatg aaaaaggaat
    19741 agaaaagagg aaactggatg tatattcggc aaacaaggaa agaagaagta gaagaagtgg
    19801 tagaaatcat taatgccgca gcggatttca ttgcttcgca aggctctcct caatggcaaa
    19861 acgggcagaa accaacaaaa gaaacgattt tgaacgatat tgaaagaaaa gaaagctatg
    19921 tattaatagt ggaagatcaa attgctggaa cagctgcatt agtctcaagc gtggatcccg
    19981 tttacacaaa aatagatggt agttggaaag aatcaactgg tccttattta tcgattcatc
    20041 gtgtcgcttt agcagaaagt actcgaggaa aaaaactagc aaaaccgttc ttagaacatt
    20101 tgttgacgat tgcagccagc aaaaatatcc aagatatccg tatcgatact tatccagcta
    20161 atgaaccgat ggaaaagacc atctattctg ctggattcac ttatcaaggg atgattgaat
    20221 ttccttttga acacggcgaa agaaaagcat atcaaataat catcaattga ctataaaatc
    20281 atctgagcaa agataaagac attaacacta ctgaaaaaaa caatcatatg atagccaaga
    20341 ggttgtaaca gaagtggcca gcttcaaaaa acaagaaaga atttccgaaa atgcttctca
    20401 aatttttgtg gaattttagc gtattttacg aaatagctgc tttctgaaac tcccttcatc
    20461 gagaggtcgt gacatagctt ttgtcacgac ctctcggctt atcctacgag tattattcga
    20521 tacatttata tcaataatcc tttttttatt ttatctgcag cttcttggaa cgctccatcg
    20581 tttgacacga ctaaaagtga ggggatagct ttcaaacgtt tcaactgtgc taaatcttct
    20641 acataaaccg ccagtctggc tgaaatctgt tcagatgaat ctccacgttt tttcaggcgc
    20701 gattgcaatg tttctttaga gatcgtaata aagataggac ggactaccgt cccgaaatac
    20761 tcatataaat gccaaaaacc atttgctgtc aaaacggcgt aacaatttcc tactgtcagt
    20821 ttctttttaa tttctgcttt actggaacca tatttatgtt gagcatattg gtcccactct
    20881 gcaaactcat ttcccttgat catctgattg aatgtattgt cagaaacaaa ataataatcc
    20941 tcttgatctt tttccccagg tcttggtgat cgattcgtgt aagtaatgat cttttgttcc
    21001 ggctgaaaaa tagcttctgc gagtgaagtt tttccagaac cagaaggacc gataaataca
    21061 tagcaataat tcattgtcat ttctcctttg atgattgaac aagtgaaaaa gctttggaaa
    21121 atatttccaa agcttttaaa agtaactttg cttttttgta tattatacca gcaagccagt
    21181 cacaatagca cttaaaaagc tgactaaagt cgctgttagc aaaattttca atccgtgttt
    21241 tgcgacaaga tttccttttt cttcatttaa tcccttcatc gcaccagaaa tgatcccgat
    21301 agaagagaag ttggcaaatg aaacgaggaa gacagatata attgctgttg tacgctcagt
    21361 aaaatggaaa ttaccatttg ctaattctgt catagcaaca aattcatttg taaccagttt
    21421 agtcgccatg atactaccgg catccactgc ttctttccat gggattccgc tgatgaaggc
    21481 aagaggggca aagatgtaac ctaggatctc ttggaaagtg atgccgaata tcccgtggaa
    21541 gatagcattg atcatggcaa tcaaagcaac aaaaccgata agcatagcag caaccgtgat
    21601 cgccacatgg aaaccatcaa gaatgtattc tcccaacatt tggaaaaatg tctgttcttt
    21661 gttttcttca atgatcagct catcttcttt ttcttctaat acatagggat ttacaattga
    21721 agccagaata taaccgccaa ataagttcaa aacaagggca gtgatcacat atttagaatc
    21781 gatcagtgcc atataagatc cgacgatcga catagaaact gtagacatag ccgatacact
    21841 catggtgaac aaacgtttct cagataggaa gggtaattcc ttttttatgg aaataaaaac
    21901 ttccgattgt cctaaaatag cagaagctac tccattataa gattccaatt tgcccatccc
    21961 gttgatttta cttaatccta gaccaacagc tttcataaat aatggaagaa tcttgatata
    22021 gcgtagaata ccgataatag cagaaataaa tacaattggc atcaatacgg aaagaaagaa
    22081 aggtgcactt cctttatttg ctacaccggc aaaaacaaag tcaacacctt gcccagcaaa
    22141 agcaagaaga tgatcaaaca tttttgccaa tacagaaaca accgctgtac caaaagttgt
    22201 acgtaataat acgaaaccaa gaacaagctg taaaacgaac atcacaatta gtggtttcaa
    22261 tttgattgct tttttgttac tgctgatcaa aaaagcaagc cctaatgtga caaataaacc
    22321 aataatacct aataaataac tcaaataatc actcctattc caaattccgt tttattttac
    22381 ctgttttgca aataaaatac taatctttag tgaaattttc attacttttt tagagtgaaa
    22441 cgcattcatt ttccattaga aaataaagcg aacaaaataa aaggtttaaa aagtgtaacg
    22501 tcttagtaaa gtctataaaa aaacgaaact atcgattaag ctaatagttt cgttttcaac
    22561 atttataagt aaactttttc tattggtaat atagattttc ggaagggtag acaggtgcgc
    22621 aggtaatatc gattcctagt tttttcaatg tttgttcatc atttttattg accatcacag
    22681 tagagtgggc ttgtgctccc tcaagttcag ctagtttatc ataagcaact tgagcgattg
    22741 gattcgttac tgcactgatt gataaagcga tcaagacttc attgatgctt agtgcatcca
    22801 aagaggaatg aagtgtcttt gatttcaatc cttgtattgt ttcaagaact aatggtgaca
    22861 ataaaggaat gtcatctgcg atatgggcta aatgttttac tgcatttaag ataactgccg
    22921 ctgacgcatc catcaatgaa cttgtacgcc cggtaatgat gacttcatct ggtaattcga
    22981 ttgcaatcac agcagaagga tcttttgtag gactttgcgc ttgaatctta tctgcatagt
    23041 catgggcagg gactaccacg tttcggtctt caggacgtaa accgacttct tccataatca
    23101 gtttgattcg attaactgct tcttcgtcga tcaatccttt tttgaagtcg cattctgttt
    23161 ggaaataacg acggatgatt tcttgtttag aggcttcttg gacaacggta tcatccacga
    23221 tcccaaatcc tacacgattc acacccatgt cagtaggaga ttgataaaca gattctttac
    23281 cagtaatttt ttcaataata cgtttgatta caggaaatgt ctcaacatcc cgattatagt
    23341 tcacagctac tttattgtag gcatcaaagt ggaaggaatc gatcatattg acatctttta
    23401 gatcgactgt ggctgcttca taagcaatgt tcagcggatg tttcaacgga acattccata
    23461 ctgggaaagt ttcgaacttg gagtaaccag ctgcatgtcc tttttggctt tcatgataca
    23521 gttggttcaa ggaagtagcg agctttccac ttcctgggcc aggagctgtc actacaacga
    23581 ttggtttagt tgtttcaata tactcatttt ttccaaaacc atcttctgag acgatttttt
    23641 ctacgttgac tggatagtct tcgatttctg agtgtttata gaccttgatg tttcgtcttt
    23701 ctagtttatt gataaagact ttcgtggcag gctgtccact atagcgagtg ataacgacac
    23761 tgttgattaa taagccataa ccacgtaatt catcgattaa ccgcaagata tccatatcat
    23821 aagtaatccc ataatcacca cggatcttat tacgttcaat atctccagca tagacgcaaa
    23881 tcagtatttc agcttgatct ttcaattttt gcagcaattt gattttcgca tcttcttcaa
    23941 atcctggaag tacacgtttc gcatgcttgt cgtcaactag cttcccacca aattctaaat
    24001 ataatttgtc atagtggttc actcgttcca aaatatacgc tgactgctct tcaatatatt
    24061 tttctgaatc aaaaccaatt tttttcacta ggttccctcc ttaagtacta aagcattttt
    24121 tcattttttt gaggattcgt caactggaag agcgaaattc ctcaaaaaaa caaagaaaat
    24181 ggtttaaacc aacttgtcta gatagttatt cattggtatg atagtttttg ataaggaagg
    24241 tgctacatga aaaaaagaca gaaaaagaaa aacgcatata aacactatat ccgttcgatt
    24301 tttaccggtt atgaaagaat gcttgaagat cccgaattgg aacaattgac gtttacttat
    24361 ctaaacgaag aaacgcagct aactcgtgat gagcatcagc gcattcattt cacaacaaga
    24421 gatctgccta gtaaataaaa aataagataa aggtcatcgg attcgattct gtcctatcag
    24481 atggaaagac tagttgatcg aaaaaaaggc tgtaccaaat tttggtgcag cctgttttat
    24541 ggagcattgc ccgctctttt ttataacaat tttttacaga aaatggtact attgaagtat
    24601 taataagtga aaaacagatg agtaagagga ggcaagtagt atgaaagagc cagatacaaa
    24661 tgcaaattat gagccagacg aactagagga gacgaatgcg gatcaggata caggacaaga
    24721 atcgtcttct ttttacgata tcaaagaaaa agcaagcatg aatctttccc aattattaga
    24781 agacttacag gcttttgaaa aagctgtccg cgaagaagat atccctgaga tctaccgttt
    24841 atacaatggc cgattgaatg aagaattaaa aagaagctca aatgaaaatc acgagatcga
    24901 tcaattgctg atgcgtaaga tccatgatcg ttttttacag gaatttcctt ttatggaaca
    24961 agtagaaaac atctcgccaa cgatgagcta ttacaaaatc ggcacttatt accatgatcg
    25021 gcccacggtc ggtattgatg caagtctgcc agaaattttt gttttgccta agatcgatga
    25081 agagtgggag aaatattccc aaccggagtc aacggactat gacaaaaaaa tcaatgagct
    25141 agacgcaaag gtgatcactg ctcagacaga aattgaacgt ttagatgaac aaataaaaga
    25201 aatcaatcga caaatgactg atttgaacga caataaagga ttcttgaatc gtaaaaaaat
    25261 cgaggaagaa attcaagaat tagagaagaa gaagcaagta ctatctaatg aaaaattagg
    25321 ctggcttcct tatattgaga cgcctgaaac aatccagcag caaaaagaag cattgaagca
    25381 agaagcaaga gcagaccagc ttcgtgcagc catcgtagaa aaagaacaaa ggcaaatcag
    25441 acgctatttc ggtagcaaag agggattcgg tcaagcaatc catgaattct tgatgaatta
    25501 tcttggcaat gagaatccta ataatcaatc taatgaagga ggttccgagt atgaatgaac
    25561 acgatgaagc agaatggaca aaaaatgaag aacaaacaga agatcatgaa gaaacgaaca
    25621 cttcattgga agaacaaaca agtaatcctc tggatgacac agaagaaatc cctatgctag
    25681 aggaaacaaa aatacacgaa aaagaaaaag taactccgac gaatacgaat actgcagaaa
    25741 acacccatgc ttacgaaata actgaaaatc ccgggtattt agaaggaaaa gagcaagatc
    25801 atcaagatat cgtcagtttg attgaaacag cggaagcaga attagcggat ttacgtatga
    25861 gaaaagattt gatcgaatca gattttccac aattattaga atcatatgat cgttttatat
    25921 tgaatgaaga accaatctcc acgatcggaa agacaagcct gatcgaatac ttcacgtatg
    25981 aattactgaa accgttgaat gatattggtc tggaacggtc aaattcagct tatgacacat
    26041 ggaaaacaag acatttttcg atcaattata cgttgaatgg acaaaacgaa ttagtctttt
    26101 cctttgtatt accgaccatc gatcaacgat accaagtgga atcgatgagt ctcctaaacg
    26161 tatcaccaga gacaatggaa atcaatgtcc aagatgaccg ggtgctttct ttgatccgac
    26221 actggtcagt ggatcgagta ttctctgcag ggcagatcac gatattcaat cacaaattga
    26281 atcaattatt agcccatgca agaacacttg gttttaccgt taatcagaca ttactagaca
    26341 atacgaagcc acttcgtttg aatcttcaga gcgaatttga attgacgaat gaagtattgg
    26401 atgatatttt tatcgtggca atgaaaaatc catcctacga tatggaaaag atcaacgaaa
    26461 ctacttatca agtacgatta gataaaggac agtctttaac gataacaaca aacgaaaaag
    26521 atcaaacagt cttgtcgatc tcatctggag actacccacg atctgtcatt gatttcttta
    26581 tcaattacga attcttggtc ccattaatgg tgagaaagtc ctgattggat aagttggatt
    26641 accagatgtc attcagataa ttctgttcca aaggacttgc ggcagttatc cagaataatt
    26701 gtcacaagtc ttttctgtat gtactaaatg gacgtgaata acttgctgat gaaggaggga
    26761 tttcattagg aatgtccaca aataaaatag cacagcttag cttattgtct gctgcttgtg
    26821 tagcaggtag gatcgctttt caatttattc cgaatttcca gccaatgact gctatttttt
    26881 tatttatagt actttactta agcttaaaag atgcgttaat tgtcatgagt ctttctattg
    26941 cgatcagcag tttttatttt ggtgttggac catgggtcat cggccaatgg atcagctata
    27001 ctgttgttct gagtttgttt tcaatcgtct gcttgagaaa aacagtgcaa aggaatctat
    27061 ggttaaaggg agtctgtttt tttctggcgg gcatggttta cggaataggt atgaccgttt
    27121 ttgatacgct gttatatcgt ttgcctcaac cttggatcta ttaccttcaa ggggtgtcgt
    27181 ttgatttgat gcacagtata ggcaatgttg tgttctttct tgtttttctg ccagtcgtca
    27241 aacgttttta caatcattca ggaggaaaaa atgaaaaaaa taatttatag tttgcttgca
    27301 atgagtgtca gtatactcat tttgacaagt tgcagtacaa cctatacaca atcggcttcc
    27361 aaccaaaagg aggaagtaaa aacatcgcaa aaagcaacaa tcacgttaca agaaaatggt
    27421 caaaactttt cggaaaaaga agtgttcttc aaaaaaggcg atgatttact gagtattttg
    27481 aaacgaaatt ttgaagtcaa agaagataac ggcttcatca cttcgcttga agggcactca
    27541 caggatgaga aaaatcaact ctattggatg tttactgtgg acgggaaaag tgctactact
    27601 ggtgccaaag agatcaaatt gaatgatgga caatcggtca ttttcaattt gtctaaacta
    27661 taagtttcat aaaaaactgt taaagggcga atattgccat aattcagtga gttcagttga
    27721 tgaaagaagg aaaataacat ggaaaatttt aaaaaaagcg gaatgttttc cgtaattgct
    27781 gctctagggg ctaatattct agttgctatc agcaaatttg tcggctatgc aatcagtggc
    27841 agtgccgcca tgctaaacga aagcatccac agtatcgttg attgcagcaa tcagattttg
    27901 ctgttattcg gtgataaaca agccaataaa ggacagagcg agttgcatca atttggtgaa
    27961 ggacgagcaa aatacttttt cagtacaatc gtcgcaatga tgcttttctt cggcggtggg
    28021 gcacttgggg tgatggaagc ttttgaaaag ttgacgcatc catcccatga agtgggtaat
    28081 ccgatcattg ttatagcaat actgttattt ggtttggtaa tcgaaggaag ttcgttacgc
    28141 gtggcaatga aagaaatcaa agaattgaat actgaaaaac tttccacgat gcgctttttg
    28201 cgcgaaagtc gacacagtga aatactgatt atttttacgg aagacttgtg tgctgtaatc
    28261 ggtttggtct tggctttagc aggaacaata ttgacttatg caaccgggat tgctgcattt
    28321 gatgcgatca gcggattgct gatcggtttc ttgctgatgg gagccgccat tttcctagca
    28381 aaagaatttt atagtctgat catcggtgaa agcgtgacag caagcgattt atcaaaaatc
    28441 aagttagctt ttgaacgtcc tgagatcagt cggctgatcg atataaaaac agttcattta
    28501 agtccaacgg agattttagt tgcagcaaaa gcagatatcg ttggtaacaa agagaatgaa
    28561 gcttactcgg tcatcaatga tattgaaaaa aatatgcgtc agtctcttcc tgacaagaag
    28621 atgtatatat acattgaaac agataaatat gacccaaatt attctcgtaa ataa
//
LOCUS       DACDUU010000030        28603 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.030, whole genome shotgun sequence.
ACCESSION   DACDUU010000030 DACDUU010000000
VERSION     DACDUU010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.030"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            237..1877
                     /locus_tag="IWL30_001377"
     CDS             237..1877
                     /locus_tag="IWL30_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAP9706153.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2031..3353
                     /locus_tag="IWL30_001378"
     CDS             2031..3353
                     /locus_tag="IWL30_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAP9706154.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3415..3846)
                     /locus_tag="IWL30_001379"
     CDS             complement(3415..3846)
                     /locus_tag="IWL30_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9706155.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3910..4746)
                     /locus_tag="IWL30_001380"
     CDS             complement(3910..4746)
                     /locus_tag="IWL30_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAP9706156.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4936..5238)
                     /locus_tag="IWL30_001381"
     CDS             complement(4936..5238)
                     /locus_tag="IWL30_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAP9706157.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="IWL30_001382"
     CDS             complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="IWL30_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAP9706158.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5692..5958)
                     /locus_tag="IWL30_001383"
     CDS             complement(5692..5958)
                     /locus_tag="IWL30_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAP9706159.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6212..6937)
                     /locus_tag="IWL30_001384"
     CDS             complement(6212..6937)
                     /locus_tag="IWL30_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAP9706160.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7303..7373)
                     /locus_tag="IWL30_001385"
     tRNA            complement(7303..7373)
                     /locus_tag="IWL30_001385"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7339..7341),aa:Gly,seq:ccc)
     gene            7490..8242
                     /locus_tag="IWL30_001386"
     CDS             7490..8242
                     /locus_tag="IWL30_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAP9706161.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8258..8737
                     /locus_tag="IWL30_001387"
     CDS             8258..8737
                     /locus_tag="IWL30_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAP9706162.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8988..10568)
                     /locus_tag="IWL30_001388"
     CDS             complement(8988..10568)
                     /locus_tag="IWL30_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAP9706163.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10561..11304)
                     /locus_tag="IWL30_001389"
     CDS             complement(10561..11304)
                     /locus_tag="IWL30_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9706164.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11480..12256)
                     /locus_tag="IWL30_001390"
     CDS             complement(11480..12256)
                     /locus_tag="IWL30_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9706165.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(12504..13340)
                     /locus_tag="IWL30_001391"
     CDS             complement(12504..13340)
                     /locus_tag="IWL30_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAP9706166.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(13351..14280)
                     /locus_tag="IWL30_001392"
     CDS             complement(13351..14280)
                     /locus_tag="IWL30_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP9706167.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            14472..14825
                     /locus_tag="IWL30_001393"
     CDS             14472..14825
                     /locus_tag="IWL30_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP9706168.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(14899..15555)
                     /locus_tag="IWL30_001394"
     CDS             complement(14899..15555)
                     /locus_tag="IWL30_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP9706169.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            15781..17715
                     /locus_tag="IWL30_001395"
     CDS             15781..17715
                     /locus_tag="IWL30_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9706170.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(17801..19024)
                     /locus_tag="IWL30_001396"
     CDS             complement(17801..19024)
                     /locus_tag="IWL30_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAP9706171.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(19018..20916)
                     /locus_tag="IWL30_001397"
     CDS             complement(19018..20916)
                     /locus_tag="IWL30_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAP9706172.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(20910..22028)
                     /locus_tag="IWL30_001398"
     CDS             complement(20910..22028)
                     /locus_tag="IWL30_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP9706173.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(22111..22944)
                     /locus_tag="IWL30_001399"
     CDS             complement(22111..22944)
                     /locus_tag="IWL30_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9706174.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(22937..23854)
                     /locus_tag="IWL30_001400"
     CDS             complement(22937..23854)
                     /locus_tag="IWL30_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9706175.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(24081..25205)
                     /locus_tag="IWL30_001401"
     CDS             complement(24081..25205)
                     /locus_tag="IWL30_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9706176.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            25388..26296
                     /locus_tag="IWL30_001402"
     CDS             25388..26296
                     /locus_tag="IWL30_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9706177.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            26437..28050
                     /locus_tag="IWL30_001403"
     CDS             26437..28050
                     /locus_tag="IWL30_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAP9706178.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         8892 a         5622 c         4868 g         9221 t
ORIGIN      
        1 ttctatttat ctatctaaca tatcctttat tcttctactt ttctttcttt agcatataag
       61 agatacgcat agtaatcaag caaggattgt tgatataaaa agaacaaata ccgctatttt
      121 gatttctttt gactctatta ctcatataaa taaaatttat tatacctgct ctttttgact
      181 atatcctcat ctgactccta tacttcgttt gtattaaaac ttaggaggaa tcattcatgc
      241 ttacagggct aaacttatta aataatcctt ttctaaataa aggaacagct tttacaaaag
      301 aagaacgggc aaaatacgga atcactggta tgcttccaag tactgtccaa acattggaac
      361 aacaatctgt ccaagcttac ggtcaatact taagcaaaca gaccgatttg gaaaaacgta
      421 tcttcttaat gaatctattc aatacgaacc ggacattgtt ttataaattg atgggcgaac
      481 atttagttga attcatgcct gtcgtttatg atcctgtcgt tgcggattca atcgaacaat
      541 acaacgaaat tttcttgaaa ccacaagatg ctgtatttct ttcaatcgac gaaccagaaa
      601 atatcaaagc aagtttgaaa aacgcagcag atggacgcga cattcgtttg atcgttgtga
      661 cagatgcaga aggaatctta ggtatgggtg actggggtgt gaacggtgtt gacatcgcta
      721 tcggtaaatt gatggtctac actgccgcgg ctggtatcaa tcctgcacaa gtcttgcctg
      781 tatcaatcga cgctggtact aataacgaag aattattgaa taatccatta tacttaggga
      841 atcgtcatgc tcgtgtagaa ggcgaaactt actacgaatt cattgatcaa ttcgttcaat
      901 cagctactga attattccca gaattgttat tacattggga agattttgga cgaggaaatg
      961 ctgcaacgat cttagaaaaa tacgaagata aaatcactac tttcaatgat gatatccaag
     1021 gcacaggaat cgttgtacta gctggtgtat taggtggatt gaatatatca ggtgagcaat
     1081 tgaaagatca aacgatcttg acatttggtg ctggtactgc tggtgtaggt atcgcaaaca
     1141 tccttttaga tgaaatgatc cgtcaaggtg tacctgaaga agaagcacgc caacatttct
     1201 atcaagtaga taaacagggt ttattgtttg aagatacaaa aggattgact cctggtcaaa
     1261 tcccattcgc tcgtaaacgc tcagaattca ctaatgctga tgaattgaca aacttagaag
     1321 cagttgtaaa agcaatccat ccaacgatca tgatcggtac ttctacccaa cctggagcat
     1381 tcactgaaga aatcgtgaaa gaaatggcgg ctcatacccc acgtccaatc atcatgcctt
     1441 tgtctaatcc aacaaaattg gcagaagcaa aagcaaaaga tttgattgaa tggacggacg
     1501 gaaaagcttt agttggaaca ggtatccctg ctgctgatgt ggaatataac ggtgtaactt
     1561 accaaatcgg tcaagcaaat aatgcgttga tgtacccagg tttagggctt ggtttgattg
     1621 cttcaacagc tacacgagta aatgctgaaa tcatttctca agctagtcga gcattaggcg
     1681 gaatcgttga tgtaacaaaa ccaggagcag ctatcttgcc tccagtagca aaaatcactg
     1741 aattctctca aacgattgct gaaactgtcg caaaatcagt tgttgcacaa aaattgaatc
     1801 gtgaagaaat cacggatatc aaagaagctg ttgaatctgc taaatgggtt cctgaatata
     1861 aatctttaga agattaagat tgttatagta aaagaacata cagaaacttt gtatgacgtc
     1921 taaaaagaaa aaagcgactg cggcaaaaca gcagccagcc aactactgct cttttgccgc
     1981 ttctctttgg cttttttcat acgatacaga ataagaaagg atctgtaaaa atggcacaaa
     2041 aaacaacaac cgcaactgca gttgaggaaa agaaaaaaga agggttttgg tcaacaaaaa
     2101 ttaccggcgt aagtttgccc ctttatttga tcatggtcct tgtcctgatc attgcaatgg
     2161 ctttaggaaa attaccgacg aatatgattg gcccaatcgc tgttttggtc attttcggta
     2221 atttatttca ttttatcgga acaaaaatcc ctatagtcaa aagttattta ggtggtggct
     2281 ccgtttttgc gatctttgcc tctgccgcga ttgctacatt tgggatctta cctgagtatg
     2341 tagtaaaatc cacagaaaac tttgtcagca atatgggctt tttagacttt tatattgctg
     2401 ccttgattac tggtagcatc cttggtatga accgtaatct attgatgaaa gcttctgttc
     2461 gttttattcc cgttgcactt atttcaatgg tcgtctcctt ttttgccgta ggactcgtag
     2521 gtatgcttat cggaaatggt tttgcaaatt ccgttttata tatctcattg cccgcaatgg
     2581 ctggtggtgt tggagccgga gctgtacctt tatcaacgat ttatgcaaat gttttaggta
     2641 cggacgcttc ttctatcatt tcccgcctga ttcctgcgac agcaatgaca aatgtcctag
     2701 ctatcattgg tgcagcgtta gttgctcgtg cgggacaatc aatgccaaaa ttcaacggta
     2761 atggtaaatt aatggaaaaa ggcgaccttg gcgatggaaa acctagaaat gtaaaacctt
     2821 ccattccaca attaggtgtc ggtttgatgg tatcattgac tttcttcatc ttaggacaaa
     2881 tctgtaacta ttttgttcct aaagtccatg cttacgcatt catgatcatt atcgttgtta
     2941 tctgtaaaat cgcaaaccta ttaccagaat attacgaaga tgcagctatc atgttcaaca
     3001 acttgattgt caaaaacttg acagcagctg ttcttgccgg tatcggtatt gcgttattga
     3061 atctaaatgt attggcttct gctttgacat ggcaatttgt tgtcttatgt ttgacaagtg
     3121 ttatcgcgat ttcagtagtt tcaggatttg tcggtcgttt gtttggttta tatccaatcg
     3181 aatcaactat tactgctggc ttatgcaaca actcgatggg tggtacagga aatgtagctg
     3241 tcttatctgc agctaatcgg atggaactta ttgcttttgc tcaaatgggc aatcggcttg
     3301 gtggtgccat cgttttgatc atctcaggtt tcttgatgca attactttct tagtcaaaaa
     3361 aaatacctga tcatcaagta tcggcttgat gaccaggtat tttttgataa aaatttacag
     3421 gattttcaaa aatctttttt gcattttatc ggaatgataa ccgatgcgtt cataaaatcg
     3481 gtgtgcttct actctgtatt ctgcagaatt aagtcttatt gctgatattc ctcgtctttt
     3541 cgcttcttct acgccagtca tcaaggcctt tccgattcct cttttttcag catgtttaga
     3601 cacagcaagt gccataatat tgaacatcgg atcgctgaaa atcgattcat aggactccgc
     3661 ttgtacatag ccagctgctt ttttagaagt aggatcttca tagaccaaga aaaaattttt
     3721 ttctggctcc ttcaacagtt tcttcatttg tctcaccgta acggttaatg gtacatcata
     3781 tcctaattgt tccgcattga tttctcgtag tgcttctgca tctcctaaac gcatttcccg
     3841 caacatccaa atcaccagcc aatttttatt tttttaaatg aattttttta tcacagtata
     3901 ctcctttttt cacttaaaaa aagacttttc agtgattttg acttgcttga atccagatcc
     3961 ttctaatgtt tgcaagatga cgaaagcatt gtaaagattg atcaaaagaa ccacgatgac
     4021 aatcgcttga aaaataaagg tcactttttt tggagacaaa accctgctaa atcttgctcc
     4081 ccacaaacct ccaataacag cagcggggat aacataaaac aacatagata aatcaaagcg
     4141 cataaagcta cttgttaaag caatcgttac caattttgcc agttgagaaa agaaaatcgt
     4201 agatagagaa tatagtgttg cttcttttat tggcaatgca aacatcagca tcaacaaaga
     4261 aacattgact ggtccaccac caatacctaa aaaactagct aaaaagccca gaaccccccc
     4321 acatataagg taccaagtcc atgacgtcaa atgaaatttt ggctgatgat gcttggtgta
     4381 gaagaacgca aaaaccaagg tcaataccgt caaaaaaatt tggatcagct gcacatgctt
     4441 ctcgttttca aataactgta aaaaaacttc gaatacaatg ttgccgacta ctcctccaac
     4501 aacagctcca ctagataccc aaccgacaat ctgccaattc agtgatttcc cgcttgatac
     4561 ttgtcgcaca gtcgaaacaa tggacatcgt gaataccgca actgttgagt aaaaagaaat
     4621 ccctgcaaca gaatgggcgc cgataagatc aagaatcggt ttgatcaaca cgcctccacc
     4681 catcccggaa atagcgccaa ctgtgttcgc cagaacgatc accacaaaat aaatcagacc
     4741 aatcatcttg tatttccttt ctcttgttta tatcttttag cgattgttac tcttttcttt
     4801 tattatacag taaaagaaac aaacgaggct tcgaaagctt ttctactttt taaggaaatt
     4861 tagtgctaaa cgaaaaaacc gggctctact tttcagagag tccagctttt cttacacctt
     4921 atcgattctt ttgttctatt cttcttccgc catgaatgta ttgaatactt cttcgatcat
     4981 atcccattct gcgtctgttt cgatttgttc taactcgcct tctgttccat cttcgttttc
     5041 gatgtatgca taagcttgta attctacatc ttcatcttct gaaacaccag ctggatataa
     5101 aagaacatag ttacgtccaa attcttcttg tccgtcaact gttagcaaga tttcgtataa
     5161 tgtttcattt ccttggtcat ccaccaatgt gatgtgttcg tgtccttcgt gttcgtggga
     5221 atggttatgt tctgtcataa gattacctct tttcttttta ttattaaaat aattgcttac
     5281 ctgagccgtc taaataattt tgtaaaatca tcacagctgc taatttatcg atgacttttt
     5341 ttcgtttcgc tcttgaagta tttgcctgct ctactagcat acgttctgcc tgaacagtgg
     5401 ttagtcgttc atcttgataa tcaaccggca aggagaatag ttctttgatc ttctctccat
     5461 atgacatgga agcttctgcc cttggtccga tggaattgtt catattctta ggtaatccaa
     5521 ctacaaaacg gtctacttcg tactgcgcca ctaattcctt caaccgatca aaaccaaact
     5581 cttcttctgc ctcattgatt cgaatgattt ctactccttg agctgtccag ccaaagggat
     5641 cgctcacagc aacgcctact gtcttagaac cgacatccaa gcccattact ctcataaatt
     5701 cactccatga tttgagaggt agtctttgac aattacttcc atgatttcat cgcgttcgtg
     5761 acggcgaatc aaatttcgtg catcacgata acgaggaatg taggccgggt ctccagaaag
     5821 caagtagccg acaatctgat tgattggatt gtagcctttt tcttccaaag cccgataaac
     5881 aatcgctaat gtttcgctca cttctttttt gcgactatcg tcaaaaccaa aacgtacggt
     5941 ttcatctgta aaacccatga ttctacacct ctttcccgca aggtaattcc agcttacctt
     6001 ctacaccctt attttactcg aagaaaggga aaactacaaa caaatattcc aaacttctga
     6061 ataaattaag aaacacgatt tgtctgattt ttcctttttt ctgtggcttt ttcaaaagcc
     6121 ctctttccat aaagtatacg ctatatttct ttgaaagtat gtttttacac aattttttca
     6181 taaagttcat aaaaaattcg aattgctgct actaattttt cgttttcgtt gcttgccctg
     6241 tctcataatt gatggtgtag ccatcttgaa cattgtagat aaagacattg aattcaattt
     6301 ttttgtcttc aatgctttct gcttccaact gcacacctct tgctaccagt tcatttccct
     6361 cgaaatgagg agtgacacga tagcgtacat gatgatttgt ttccttgata tatgccgcaa
     6421 tatcattttc gtgggcaagc atgtacggag cattcaacga acgtgtccct gtcattaagt
     6481 ttttaatgtt attattttga ccagtcaact ggaaaccaat caaatggctg cggttgtata
     6541 accattgtcc gtcacttaat ttcttttgtt tccaacctgt cggatcaata tacaaaggtt
     6601 ctctcttttc agtaggaaac aagtcttttc ccaacatcgc attggcttgt cctactctgt
     6661 tcaatttgtc tatatttgaa tagctttccc atgtcccttt ggaaagacta agatcctctt
     6721 ttgtaaactc cggctgattc ccattgattt ctatttcttg atgttttcca tcatattctg
     6781 gcacatccgc tacttgagaa gcttgcggtg tggaatcaaa aatggttgta atttgttgga
     6841 agatatctga atctttttgt gtggtggaaa taccgagtgc ccctgcaatc acagcagcga
     6901 cgatccccac gatcgccttg gttgtttttt tgctcatttc tttactcctt tactaacagt
     6961 tccctaaatc ttaacatatg ttcccctata aataaatact ggaaagaaat agaaacagta
     7021 aaaaaataaa accagttttt agacggctta agaatacaaa aaaagagacg gattcatcgt
     7081 ctctactcaa tcacaaaaca ctatttacaa ggtatttatt atcattcaaa aaatttttct
     7141 taatactcaa aactaaacgg cttctgtacg acctcttggt aaacagtgtt caaaagcttg
     7201 catctgcggt aataagtcca accaagtcaa aaacataaga agcttttttg cttgactatt
     7261 cttattactt gatgcagggc agctttttca cagcctcttt taagcgggtg acgggaatcg
     7321 aacccgcgac atcagcttgg gaagctggga ttttaccact aaactacacc cgcgatacaa
     7381 aaacagtata cgcctctttc actccttcct caagtttttt tattctttac gcttccgata
     7441 tgttttgtag taaaataaaa ctaatcaatc aattattgga gaagatgcta tgacgggaaa
     7501 agaattagat ctgttgctgg acacttgttt actcgctggg aaaatcatga tggaaagcaa
     7561 tgcggagatg tatcgggtag aagatacgat gagtcgaatt gctttagctt ctggaaattt
     7621 ccgtttggtc agctatgtca ctcaaaccgg tctctttatt ggtcttgatc gtacctcgac
     7681 catccgaatg gaacaaatta ccaaccgttc gattaattta gaaaaagtag taaaagtaaa
     7741 tgatctttca cgtaaatatg ttgcttcaga gctaacatta gaagaacttt actgcgaact
     7801 gcaagcaatc gaccgagacc gttctttctt tcctgtctgg ttgcaaatcg ttagcgcggc
     7861 agtgatcagt ggaacaatta tggtgttatt tggcggaaag atgaccgatt tacctgtgac
     7921 tttagtcatt ggaggattgg gatacgcagc ttatctatat agtttgaaat tttttagaat
     7981 caaattttta tcggaatttt tatcttcttt attgattggc atagccgcta tctacagcgt
     8041 acgttttggc ttaggaagca atcaggatct aatcattatc ggatgtgtga tgcctttagt
     8101 tcctggagtt caaattacga acgctatccg tgatttgcta gctggacatt atgtctcagg
     8161 agtttctcgc ggaacagaag cgatgatgac tgctacgatg atcggttttg cgattgcttt
     8221 tattttccag cttttttatt aggaaaggaa cacaagtatg gggacgatat ttattcagtt
     8281 ttcttttagt tttttagcga ctgcggcatt tgccgttatt acaaatgtac ctaggcgttc
     8341 attgatttgt tgtggtcttt ctggtgcttt tggctggatg atctactgga tctgtgttca
     8401 aataggcgga tctgctgctt ttggttcatt gcttggttct ttaggagtag cattcatcag
     8461 cgatcttttc tctaaacgtt tgaaaatgcc cgtaacgatt ttcaatattc ccgggatggt
     8521 tcctctagtc cctggtggtt tagcttatca agcggttcga aatctcgtta ctgaatccta
     8581 tcgagaagcg gctcattatg cggtccaagc aattatgatt gctggagcaa tcgctttagg
     8641 tcttgtatta tcagaagtct ttaaccataa catccgtaat ttcaaagtaa agcgtgacgc
     8701 aatcatgatg aaacgaaaaa aacggctttt taaataaacg aagtgtaaca aagaaaaggt
     8761 tgtgaaagag acgttcagcc ttgagcgatg acaaaatatc tcacaaacat tgcgacaagt
     8821 aaccgcagga gcaataagaa gtaattatga gtattaaaaa aagttttgta aaaatagctt
     8881 ctcacatttt tacataaaat tgacttaata ctgagaaact gacttttgaa caccctctat
     8941 ctgtaaaaaa ggggctgcga catgattttt gtcacggccc ctcttcttta ttcaaatttt
     9001 ttccaaaacg ttcgttgata ataaacgatc catagagcgg aaagtattag aactaaaccg
     9061 atcacaggaa tattcactaa ccaaaatgga taaaacaaag ccaacattcc gtataagacg
     9121 ataagaataa tactgatgat caatgaaccc atcatagttg cgactaaacc caaacttcct
     9181 gaacctctgt taaataattg gttaatattg gtccagttca gcagcagcaa ccgataatct
     9241 ctagcgaaat aacgtagtga caataagtag cagccgaata aagaaccaaa tagcaagcta
     9301 gggataaaaa acaaaggcag ctgaaaagac agtccgctta ataatgcgat cccaccagtt
     9361 aaaaaactct gcaatatcca tccgaatctg aacttttctt ttaaatattg gttcatagag
     9421 attggcaaag aacggataaa cagaaaattt tcttgatcca aagaaataag attgctgata
     9481 aaagaagtct gattaactgt taagaaagcc aatgcgatcc ctaccaggaa aacaacaccg
     9541 attaacttat tatctaaatc agttaatttt atttcgcctc ctagggcaaa agcaagaata
     9601 aaaatgattg gagttaacaa ggaagaagaa agaacttgca taatcaaatt aggttctttt
     9661 actaattgag cattgtaact aaataaaagc tgatgcacat tttggttctt tttatgggtt
     9721 ctttttattt tgaactcact gtttgaatca gtcagttgct ctgtaagctt tggtactatc
     9781 agctgtttta cagcaaggaa aaatcccaga gccgtgatag ctagtattcc ccaacccaat
     9841 aaaccttgtt gagaaagtgg ctgactggca gcatcataga atggcaataa tatcgaaatc
     9901 ggatgccgat ctgttaactc attttcaata gtccccgttt gatgattcat ccacatgatt
     9961 ccagcaaatg cgatcagcat cgagctgcca agcaagatac tagaaactgt ttttctatgt
    10021 tttttaaaca ctgctgttcg agccaaacca aaaacaatca aactagccag acaaaaaaga
    10081 ataatcaaaa atagagaaaa caaaaggatt gagataatga tagtaagcaa aatccctaga
    10141 tcagctcgcc atccggttaa aataaacatg actagaagcg gaaaaacaaa aggaatgatg
    10201 gtcaaagcaa ctaccaaaat ctttgctgaa aaaacctgtt cttgtccaaa aggcagaggc
    10261 aaaaatactt gcaggtcatt tccttcaaaa aagatattat aaatgactga tatactttga
    10321 gttaaaccta acataccaaa aagtgccacg taataagtaa agaaacctgg caatttaaca
    10381 aagttcatag caaacatagc aaatccgtaa atcagtaaaa aaatcagccc tgaaaaaaga
    10441 ttttgagaca aaagatagat agttaatcgc tttccctttt ttcctttctc tcttgcttta
    10501 ttcgtgagct gtggattaac atataaaaga ttaactcgaa ttaattctaa taattgtcgc
    10561 ttatacatac ggtccatctc ctgtcgcttg ccttccagcc attttcaaat aaatcttttc
    10621 tagcgtttca tcaggtgagc cattcaataa ttcactaact gccccctgat aaatcaattc
    10681 acctttcttc aaaatagcca gtttatcgca taactgttgt gcggtatcca atacatgtgt
    10741 agaaaaaatg actgtttttc cttttgcggc atgagccttc atcatttctt tcaaatcaaa
    10801 tgctgcttga ggatcaagtc cttgcaaagg ttcatccaat acccaaatat ctggatctgg
    10861 cagcaacgtg ccgatcaaaa tcgttttttg acgcattcca tgtgaaaaac ttgcgattgt
    10921 ttcattttga tgactataca tatcaaaaag cgctgtcaat tctgctaaac gcttttcttt
    10981 tttttgtgtg tctaattcat aggcagctga aatcaagtcc caatattctc cagccgtcaa
    11041 ttgtaaaaaa atatctgggg aatcaggcac ataaccgatt ttttgtttga tactcaatcg
    11101 attttccgtc aatttcattc catctactaa aatcgttccc gatgatggtt caataatact
    11161 aactaaactt ttaatcgtag ttgattttcc ggcaccatta tgtcctaaaa atccaaaaat
    11221 ttccccttgt cttatcgaga gagacaagtc ttttaatgct tgttttttcc cgtaactttt
    11281 acttacattg atgaattcaa tcatttctgt actccttttg tttgttatta ctttttattg
    11341 tacccttcgt tattggtgga gtaaatgtca aaaagagtgc ataacaaaaa aggatagaaa
    11401 cgactaggct gctacttggg ttgttctctt ctacaggaaa acactcttct aacaggtaag
    11461 atatgagtct ttgttttctt taaaatgatt cattttctat cttttcttgc ataaaattct
    11521 ttagtttatc aatacttctc tttcccatct ctagttgttc tccatttgca aatgttacta
    11581 tttcgtttac tctgctaata gatgtaatgt tttcagtatt aatgatataa gcttttaaat
    11641 ctttacataa atactcatat tcgagtgatt ttttatattt tttaattgtg ccgtttacga
    11701 gaatctcttc atctttcaat actaggccta ttttattttt ataacctggc atgctcatta
    11761 gataaaggat ttcatcgtaa tggatatagt gatcgctatt accataacgg acagttgtcc
    11821 accttttttt cgtagcatgt tgaacaggat agttttcttt ttctttcaat aagtaacttt
    11881 ggatgctttt tttatttttt tcataagtga attcttttat caaataacca ctaggcgtaa
    11941 tctttcgatt aataacagtt attgctttat tcggatgaga cgttagataa acaattttac
    12001 acttttcatt cttttctcta agcatttcgc ctagttcgat accgctataa taggcgttca
    12061 aatcgatgtc taggaaaaaa atatctgagg aatagatttc attattttta atgaattcaa
    12121 caaattgtat aacgttcaat ccctgaacga tctcaaattt ccaaggactt aataaaattc
    12181 tggattcttc caaaatttct actagcattt tttcaaaaac caaactatca tccaaaataa
    12241 atatacgcgt taccataaaa agtacctcca ctcgatattt tactatcaaa atcatttcat
    12301 acaacttttc aacatacatg ttaggaataa attgtaattc tcttcacaat cttttccaac
    12361 acctttgaaa ccgatatcaa atactttcag gatgctgctt ctaaaataaa tatcaaacaa
    12421 atgaatacat catgcaaaaa aagccagaca accgacgtcc ttgcgtgtca gctgtctgag
    12481 ctatgtttat ttattttgtt cttttaaaaa gaacgataca catactgatt tttcggagca
    12541 gagactttct cagcgtttgt aacaactaca gatgggattt cacgcttgaa tggttgataa
    12601 tagtctgatt ggatggttcc ttctatttta taccattcat catttttaac cgttactcct
    12661 tccggcatat gcgtcaacaa accaaagact cctgaatctg ccacacaatg gataatgccg
    12721 aatcggaaca agaagatatc tgtattgcta tcgttcggtg tttggtaaac aaatccttca
    12781 tagctgattt ttttgccaat gaattcgcta gggtaattat aaattagctc cataacttct
    12841 aaatagtttt catctgtgat cttgatagtt tgcttatccc catacttttc tttcaactca
    12901 ttcatttggt ctaagtagtc tgatttgttg aaataaatac tggtatccgg ctttaagtat
    12961 tgtgtctgca tctccggatc ccctactgat tctttactta ctgggaaatt aaatcctttg
    13021 gcttctacga tcgtagtgtc gagactaact gttggaaaca acgtccccac gattactggc
    13081 aaagctagca gtacataagc gattccacgt tgataaggtt ttgacaaccc atggtcatga
    13141 tcgtgctcgt gtgccttttc ttctgctttt ccgtttttga cccacaaata cagctgaact
    13201 aaagccaaaa gagcagataa aatcattgaa agaatagcta agtagcgata atggacattg
    13261 atatattgat cgagtttgcc agaaacttgc aagtacatca tcaattcgaa atacccagat
    13321 aaaatcaaaa accgcagcat attttccctc ctaaataaat aatgtgaatc caataacgct
    13381 aagggcaatc agtccgatca gcgataaaat aaagcgtcct gtaaaatatc gtttcatcat
    13441 cagtagattt ttgatatcga ccattgggcc aaagaccaaa aaggcagcta caggtgctgt
    13501 gccgaacaga cttaacaaag aagatcctat aaacgcatct gcttctgagc aaagagacat
    13561 cgtaaaggcc aataacagca tgatgataat ggctaaaatc tttgttgagc cgagcgttag
    13621 cataattcta gttggtaaat aagtctgcat cgcagctgcg attaatccgc caatgatcaa
    13681 ataacgacct gtatcaaaaa attcgtcgat tgaatgtgtc aacacatgcc cagtttgatg
    13741 gatcagctga ttttttggtt cattcttctc actttgttct tcttcatgga catgatgatg
    13801 gtgattttct gctactttag acgtgatttt ttctttcaat acagcgtctt tttgaaaata
    13861 ggcaagccag acaccaatca caagggcaac aaaaaagctg cctatcaccc gccagcttgc
    13921 catctgccaa gaattgccaa atgcgataaa agtggaaaaa atcacgattg gattgatgat
    13981 cggagcagtc agcataaaag caaacgctgt gtgaactggt acgccttttt taacaaattg
    14041 atgaacgatc ggtacgatgc cgcattcaca tgaaggaaag aaaaatccta agatcgaacc
    14101 agtaaaaata gagaagatac gattttcagg aagccatctc ttcacacgtt ctggggtcaa
    14161 aaatacttgt aaggcgcctg agataagaca gcctaataat acgaaaggta atgcttcaat
    14221 aacgatcgat aagaaaatcg tactcatctg caaaaccgaa tttggaataa aagaaaacat
    14281 gagtttcctc ctttttattt ctagttcatt cgctttgcaa atatacgctt ttattcaaaa
    14341 aaaacaagtt gctctgtaaa tttttacaca actcttattt tttaccaaag cacttcaatt
    14401 gactttttct gttattccca ttaaattaag gtctcataca tatcacttct atcataaaag
    14461 ggagaataaa aatgaaacaa accactaact tgactgaagt tcaagacata ctgcaacagt
    14521 catccgttgc cgtcatttat ctttcgatgc ctaattgcag tgtttgtcac gctgttcgtc
    14581 cacgactaga ggaacttctc acacattatg atattccggc acttcatttg gatgcatttg
    14641 aaacaccaga agtagctagt aaatttgaag tgctgactgc gccagttgtc ctgatttttc
    14701 atcaaggaaa agaagtcttt cggcaagcac ggttcatcga ctttaaaaaa atccgttatc
    14761 tactagatga actgacagca gaagatgatt ccctttctta tgaagaaatc ttccgaacaa
    14821 aataatcgtt gtccaaacag aaaaaagcga ttgaacaagt gaaagttttg ccttcatttg
    14881 ttcaatcgct ttttattttt attctgctgt attttcggaa tgcgcatttg cgtcagaatg
    14941 caagaaatag atcaaggttt gcaattcatt cgtcaaatca acattttgga tcaatacatc
    15001 tgacggagca ttcaatcgag cagcggtaaa gttcaagatt cctttgattc ctgcttccgt
    15061 taactgattg actacttctt gtgctctttc agcgggtatc gttaaaattg ctacttcgat
    15121 ttgttggatc ttgatttgtt ctgacatatc ttccattgga taaacgggga taccgtctac
    15181 gatgcgccca acgatttcct cattcacatc aaaagcacaa ctgatacgta tgctattact
    15241 ttgatggaat ttgtatttta gtaaagcact tcccaagttc cctacaccga tcagcgcaac
    15301 atttgtcaat tgatcgtcgt ttaatgtctt tgcaaaaaag tgcataagat cttctacatc
    15361 ataaccatac ccacgttttc ctaattcgcc aaaataagaa aaatctcgac ggatcgttgc
    15421 actatctact tgaacggctt cacttaattc agttgaagac acttttttct ttccagcatt
    15481 atttaaaatc cttaaatagc gataatacaa aggcagtctt ttagctgtcg cctttggaat
    15541 aatatgatct ttcacgaaat gacctccacg tattgtctaa ttacatcttt gatttctcca
    15601 tcatagcagt atttatagga aaaaatcaat tccattgctc agatattgtg aaattttatt
    15661 ctttattcat ttatttatca tttctgcaaa tctcgcagta taatggaatt tatggtagac
    15721 tactaattga aaaatagttt tttatttgag gagaattcac aaagtagaaa agaggattcc
    15781 atgatattat tacaagccaa tcagattgct cgccattttg gtgcagaagt cttatttgaa
    15841 aacatacatt tagaaatcag tacaggcgct cgtgtcgctt tagttggcag aaatggcgct
    15901 ggaaaatcga ctttattaaa gatcattgct ggaattgaag cacctgatga aggaaagatc
    15961 gctaaaaata aaacagcgac tctcggatac ctcgctcagg acacaggact tgattccaat
    16021 gaaacagttt ggaacgagat gctgaaagct tttgaacaag ttagacaaat ggaagagcgg
    16081 atgcgcgagc tagagatttt cattagcgaa ggtacgccag acacacctgc ttatagttct
    16141 tatttaaaag aatacgaccg gctgcaacac gatttttcag atcgaaacgg ctatggttat
    16201 gaaaatgaaa ttcgttccgt attgcatggg tttaaattcg attcttcatt ttatgatcag
    16261 aaaatcgata ctttatcagg cggacagcgc acacgtttag ctttagctcg catgcttttg
    16321 caacaccctg acattttgat tctagacgaa ccaacgaacc atttagatat cgagacactt
    16381 tcctggcttg agaattatct gcaaagctat actggtgcat tattgattgt ttcccatgac
    16441 cgttattttt tagataaagt agtcaatgaa gtctatgaat taagcagaag aaaaatgcgc
    16501 cattacaaag gtaactattc gaaatatcta gatttgaaag cagaacagtt agcaagtgaa
    16561 tggaaggctt atgaaaagca gcaaacagaa atccataaat tagaagactt cgttgctcgg
    16621 aaccttgttc gcgcctctac cacaaaacga gcgcaaagcc gcagaaagac attagaaaaa
    16681 atggaccggc tggatcgtcc tcaaggggat gaaaaacaag caaactttct attcagtata
    16741 gaaaagcctt ctggaaatgt agtcttgcaa atcgaagatg ccgctatcgg atatgataat
    16801 gtcaccttat ctgaaccaat caatctagat atccgtcgtc aagaagcgat tgctctagta
    16861 ggtccgaatg gtatcgggaa atcgacgttg ctgaaatcaa tcattcacca gatccctttt
    16921 atccgtggaa aagaaacgct agggacaaat gtcaccatcg gctattacga tcaaggtcaa
    16981 gctgatctgc atacaaacaa gacgatccta gcagaattgt gggatgagca tcctaccact
    17041 ccagaaaaag agatccgaag tgctttaggt gctttcttat tttctggcga agatgtagaa
    17101 aaaccaatcc ctctgctaag tggcggtgaa aaagcacggg ttgctttagc caagttagcg
    17161 atgaacaaag aaaatttttt gattttggat gaaccaacaa accatttaga tattgacaac
    17221 aaagaagtat tagaaaatgc attgatcgat tatgacggca cactgctttt tgtttcccat
    17281 gaccgatatt tcatcaatcg gatcgcgaca aaagtcgtcg aactctctga aaatggcagc
    17341 aaagtctatc taggagacta tgattattat ttagagaaaa agcaggaaga agaagaaatc
    17401 gccgaattgt tggcacaaga tgagccagaa tctgctcctg ctcccacgaa caaatctgat
    17461 tattaccaaa gcaaagaaca gcaaaagctt atccgtagcc ttcaacgaaa aattactcag
    17521 gttgaagaag aaatggcgcg gatcgatgaa tggatcgatc agttggaaca agaaatggtc
    17581 gcgccagaaa acctggaaga tcacgtaaaa ctcaatgagc tgaaccaaga tttagaagct
    17641 gctagacaag atcaagaaac aaaattgacg gaatgggaag aactaagctt ggaattagaa
    17701 gaatatgaaa attaacgaaa aaaccaaaac agaaaccatt catgctgttt tggtttttct
    17761 ttttttattt tatcaaacaa aaattccata tttacctttt tcaccaataa gaagaccaat
    17821 tactagtcat tctcactaaa gcttctaaat aatagtaatc accccaaata caaggtgtat
    17881 ttaccccatt atttttgggc ttattataaa cagactgtgt taaaaagcca tcttcttctt
    17941 ccgttgtagc atatctttct ttcaattgtc gcataatttt ccaaacagca gactggtata
    18001 agttcttttc tgttttgtct aatagacctg attctattaa ttctaataat ccacaaacca
    18061 tgatcaccat actagatgta tctttttctt ctctgctaga atcatcaaag atcaaatccc
    18121 aataacaaat ggtatcttct ggcaaccgat tcataaaata attggctaat attttgctta
    18181 aatcaaaata tttttgttct ttcaaataac gataacttaa tgcaaaaccg taaatccccc
    18241 atgcttggcc tctagcccaa caggaattat cactataacc ttgatgcgta cttccatatc
    18301 ttggttttcc atctttgaca tccatataaa atgtatgata agttgaacca tcttctctta
    18361 ctaaataatc tgctgcattt tgaatatgag cctgagcagc ttttttatat ttttgatttc
    18421 cagtttcttc actcgcccaa tacaataatg gaagattcat gcaacaatcc atgatcattc
    18481 taccttgttg tgaaggatca ctaggatctc cccaagcttg tataactcct gccttctccc
    18541 aaaaacgctg aagtaatgtt tctgcagcat caattgaagc ttccttcatt tttcgatttc
    18601 ccgttatttt ataatctgct acggttgata aagaatacaa aaaaccaaga tcgtgatgat
    18661 tagtgataat ttgacagtca atcctgtgga taaaatctat atctgctttt tcagctgttt
    18721 gttgaaataa aggatcaccc gtcttctcaa ataacaacca attaattcct gtccaaaatc
    18781 cttctgtcca accaatattg tctcttaatg gataacgtcc ttttatagga cactcgcttg
    18841 ggaattgata ctgactgtta ctgatacgtt tgattaacct ctccgtgtgt ctgcgacaac
    18901 gctcgatctc attattccat tctttttcca ttaattcttt ataacattca tttatccatt
    18961 tttctggatg tattatctct tcaaacatca ctttatcaag catttcccca ccagccttta
    19021 gcataatttt agtctttttt tattgttttt attttggata gttagcccgg ttgtcccatc
    19081 aaaatcaatt ttgggacgat tgttccatac ttctaaacta taatcccgat cttgatgagc
    19141 aaccactcct gttccgagcc aatgggttcc ttttccaaat gtaccctcta atccaggaat
    19201 aaaagtgttt tcccatgtca tcaagtttgt attgggcaaa cccataatag ctgtggattt
    19261 tcgtttaata gtatcgtcac ttaaacagat aatacctgaa aagccagctt cactcaccct
    19321 atagttttcc ttgtcttctt ctatgattat tccttctttc tcaggagaac attcaatagc
    19381 aaaccctgtc tcatatagaa catattctgt atctaattct actttgtgta cgcgaatctg
    19441 ccagccttca aaaggaataa gccaagttct gatttttgcc tgtaaagtag gttcccatgt
    19501 agaggcaagt acctctttct tacatacaat gtctcgtaca ttatttcgaa cgatgaactg
    19561 tccataggtt ttgatgctct cattcttaaa actggtcgtg atatatgtgt ccgctcgctg
    19621 aatccctagc attgaatcca ttgccatttc tgctttcaat tgattcgctc gcggtatcga
    19681 aaaaccaaat tgactagaat aagcaaattt taaatatttt tcattggtca atgtatggaa
    19741 atttggccct aattgtcccg cattcaaaaa aaatgtatgt ccattatcat gttgtatgag
    19801 caaattggct tctgaaagaa gtttagttcc ttcacgaata ggcataggca tttctttagc
    19861 cgtccaatac tcatggtctt caggtagcgc caacaataaa aaaatcttat tcagccataa
    19921 tggagataac gtactattat agggttcagt caatgtcaat tgagggtagg tataaccaat
    19981 attcaggata cctttttcat caaatatttc tttttgcatc caccatctta tattccgatt
    20041 gactaacccc ttgatttcac ctaatttaaa aggcaaaaga tcggcccaca acatagctga
    20101 ccaaaagctt gaaactgcaa aacgataaat catgcttcga ccaaaaggca catttgcacc
    20161 atcttccgca aaaaaatgaa tatagtctct agaaaaatca atagctcggt tcttatatat
    20221 ctggctaatg ggcttttctg gttctagtac gctataaatc aaaccataat aatgaaatgc
    20281 aaaaggatta taataatctt ggcgacctct tgaactgtct cgataccagc cattttgaag
    20341 ataacaattt tctatattat caaatgcttc tttcagtgct gattctttaa ctggataacc
    20401 aaatttttgg ataattgttc cgacaattat cttaaaaaat tgccaattac catctgcaca
    20461 ttcatattca aaaatttgcg agaaccaatg aataatctgg cttttttctc ctgtatctaa
    20521 ttttttccat aataattgtt catgatgata taacgctaat aacattgcgg gcatttctac
    20581 catacgctgg tctctttcat gtatttctcc ccaataatcg ggatgggaag gatctgttcc
    20641 agcttttatt cctgatagaa ttaatttcaa ccatcgttca tctaattggt caattgcggg
    20701 ccccgctccc cataaaaccc gagagaatcc ttccattcct gcaattttat ttccatatcc
    20761 taccccttga tgtatataat caatttgtgc gccacttgct cgataaaacg gctggagtgg
    20821 ttgatttatc tttttaaata agtcaaagta atcctgtctg gtccttaata gtggtactgg
    20881 atgtttcatt gtattttctc ctctttttac taccaaatag ctaattccga ctgttctaat
    20941 cgaagtaagc cttcaataaa aaaataatca gaatagatca gtggcacatt ataagtcagt
    21001 tctctatggt atgcttctgc tccgcctcca atgatcccat cttcatccat attccaattt
    21061 gcatagtgct tttctgtctt ttggagtata ttgatagcag cttcatgata catttttgat
    21121 cgtacagagg aaacatatcc tgctaattcc agaagaccgc atgcagcgca tactccagca
    21181 ctggtatcaa tattcgaatc ttttacagca aaatcaatga gaggaatata accagttttt
    21241 tcgattttct gaataaaatt tactgctatc ttttctgcta tagctagata gtacggattt
    21301 tttgtgtaac gataacttaa cgcgaatcca tataaagccc atgcatttcc tctgctccaa
    21361 caagaattct cattgtatcc ttgcccgcca agtaaccgaa tatatttccc tgattctgca
    21421 tcaaattctg cgatatgccc ggttgatcca tctttcctca ctagatactt agctgttgta
    21481 tctgcatgct gccgagcaat tttagagaac ctcggatcac cagtttccct cgaagcccaa
    21541 tacagtaatg gtaaattcat catactatca ataatcatcc aaccaggttt atggtcattc
    21601 catgctacta aatagttccc tgaaatatta tagcgtccag caagtaccgt agctgcatgt
    21661 aaccctagtt gtcgactatg tttgttttga gactgttgat aatgtgcaat agccgatggt
    21721 aagaataaaa aacctacatc atgatgaacg tctgaaaaat ggtacagggc agattctaat
    21781 aattgttctt gttgtattgc tgcctctttg aacttttctc gttttgtctt gtgaaagagc
    21841 tgccataaaa taccactcca aaaaccattg gtccaccagt cgattcctct gtcctttatt
    21901 acgtctgtat attttccgtt gtaagaaata tatggaattt tattccccat tctgtctaat
    21961 tccgcatcta tcttttcaga agctacattc aaaatttttc ttgaccattc cttcgtctgt
    22021 tcattcatgg atatcccgcc attcttttaa tttttatgaa tgtggagttg ctcctagaac
    22081 gaactagatg gttaacattc gttatttttt ttacttattt gcagctccaa tgtcattctc
    22141 cttaaattta gactgcgccc acacataaat caagaccatt gggatcactg aagttgctaa
    22201 aattagcata attgcgccat aattaattgg ctcacctgta ctagaaaccg tcatattttt
    22261 gataattaag ggaaccgtat atttatcttt atctgaaagc atgactaatg gaaatagata
    22321 ttgattccaa cttcccataa agataagtaa accactagta ataatcgtag gtgataaaat
    22381 cggtacagcg attttcgtaa aaatcaccca ctcattggca ccatcgattc tggcagcctc
    22441 catcagtgct gtaggaaatt gactgctata ttgtcgcatc ataaacactg aagttgtact
    22501 ggcaatcgaa gggatgatca cacctgcata agtattcgat aatcctaaac gactcataat
    22561 gtaaaataaa ggaatcaatg tggtaaaaaa aggcaccatc ctagatagca taactgtatt
    22621 gaacaacagt ttattcccca taaaacgata ctttgctaat gcataacccg ccattgtaga
    22681 actggctgta gctgaaactg tactgattac agcaataaca atcgaattcc aaataatcaa
    22741 gggataatta tatctcgcca tgatggcttg ccagtttttt aatagcatat ccccaaactt
    22801 gaaattgatt ccgccactta aaatttcttg attactttgc gttgaagcta caatcatata
    22861 taaatatggc atcaaaaata ttaaggcacc aaacacaata atgacgtacg taaaaattct
    22921 gctttgtatc ttttgtttag tcatttttat cacctccgaa tttaaattga atagccgata
    22981 atataacaga tacaaacacc atcgtccagg caatagcaga ggcttttcca aaatcaacca
    23041 tttcaaaact tgttttatat aaattgactc ctacgaccaa agctgaattt tctggtccat
    23101 tggaagtccc aaagaatata tttgggatat tgaaattacc caagccattg atagtggcta
    23161 aaactgtggc cattaaaatg attggcttta gcatggggat cgttaataaa aagaattgcc
    23221 gaaatttcga tgctccatct atttcagcac tttcgtatat ctctttagaa atcccttgaa
    23281 gaccagctaa aaataaaata gtgttatatc ctacactaag ccaaatcatc gtgatgatca
    23341 ttgatattcg tatccaaaaa ggatttgtta accactgtac accgttaatt cctagcgcat
    23401 tcaaaaatcc attgaataac ccagtcggat tgaaaaaagt catgaatacg gaagctacag
    23461 ccaccgttga tgtaatggtt ggaatataat agacagttct aaaaaatcct tttcctttta
    23521 actgtgctga attcaataaa acagcaaagt aaatagctaa tagcatcgtt attggaattg
    23581 atcctgccat gataatcaaa acattcgtgt aagatttaat aaacaatgga tctcggaaga
    23641 tagctagata attgtcaaat ccaacaaatt caaatgagta gccaattttc ataaaactaa
    23701 aagcaaacga gataatgatg ggaatcaaaa tcgtcacaat aaagaaaata acaaaaggtg
    23761 tgaccaatcc ataagcactt tttttatgag acgtatctgt tgcttttttc ttgctgttct
    23821 ttattgtctt gcttggtaac gaagattgca tcatcgttaa gtcctcctaa tattttttaa
    23881 tcaccagtta attttacgcc gtacttatga gcaaaatttt ctgcttcttt tagatatgaa
    23941 ttttcaaaat cacctgtttg ccaatagtta tatgcagcgg tagtcaaata agacgtagca
    24001 tctgaataag tggctggtaa aacagtattt gatgtatttt cattgatttt attcaatagt
    24061 tcatagtatt tttgtcctcc ttagtagtca aactttttct cagctgcttc tgtttgatag
    24121 gccaatgagt tagatgtagc cacgcccagc tccagtgcgt tggctaagca ttcttcatct
    24181 gtcagcataa atgtaagaaa ttgttgtgcc acttgtggat ttttggcatc tttagggaca
    24241 taaaaacttg atcctcctga tactgcacct tttcctggat tttcttttga aaaaggaatc
    24301 agtggagcca tgcgccattt gtcagatgca tcaggaaaat tcaaagacat attcgtagca
    24361 taccagccgc cagcaacaaa cattgcagat tcttgaaatg ctttctcagc atcttgtgca
    24421 ccgtagaaat gtacaatatc tgagtcgatc atttctttga ttaactttgc agcatgtttc
    24481 gcttctttag tattcagacg gatatttcct tcttcatcaa gcaaagattg attctgctgg
    24541 gctagcatgt tgataaacaa ttcttggtcc ccagattctg gcaaggcaat tccaaaacta
    24601 ccagtttttt cttttaatgc ttttgccacc tggatatatt cttcccaaga actgatttct
    24661 tcaaaatcta cacctatttc agcaaatttt tttggttgat aataacaaag tgctggggta
    24721 aaatcactag cccaaggaat aatcgtttca ttactcgttt gttgacgcaa aatattcaat
    24781 ttttgatcat aaaactgatc gccatatttg tcataaaatc catattcatc agcactataa
    24841 aaagcatctt gaaatttttc cagcacacct gctgctgaag tgtctgccat ggagataata
    24901 tccggcatat cttcgtgtgt taccaatttt ggggtgatca tttgtactgt tgctgttccc
    24961 gaagctacat tcacaatttt taagtcaata tttgctccag ttttgcggtt atagattttt
    25021 gcagcatttt caacatagtt tgtcagtttg ccgtttgcga catatacttc taatgtagtg
    25081 gcttttttta tcattggatc tgcgatgttt gctgttactt cttcgttagt ttttccacat
    25141 gcacttaagc tgaataatag ggtaatgttt agcaaagcta tcaccaattt gtatcttttt
    25201 ttcatgtcta tcccttcttt cttttcaagt tcttatataa aacgctttca cttatgtata
    25261 ttaacggaat tagtaacaca aatatttata taaaaaaata ttttacttgt cgttttggct
    25321 tttcattttt gttataatag tcattaatta taaatagaaa gctttttata taaaaagaga
    25381 aaggaaagtg gttcttttgt taacaaacaa ccaatcctcc aatcttgtca aaaaaacact
    25441 aacctattcc atagataccc atcttcattt atctgctgct ccctattttc gactactaat
    25501 aaatgaaagc gctaatttat atgctgaatt tgcacataaa tctatagaac tagataatcc
    25561 ttccctttta ttcattgata aacagcaaga agtccaaatc ttttctactc aaaaggaaac
    25621 acaaactttt atcattatcc agttttattt tgatgagcaa ttattagcac atattacggg
    25681 acaatctttt tctatagatc aagaaataac atctatcatt acttttctga aaaatgaaca
    25741 gttcaaaatt gattatctaa aacatagtaa gccagctatt tcctatgatc aggaaatcaa
    25801 ttcttttttc atactatgcc aacagatgag cattcatttg ctttccttat tagttactta
    25861 ttgtctgcaa aaaattcaat tgtcttcctt accaaggcat tatcaacagg aacttgcttt
    25921 attgacaata ccttcaaatg agaaagtcat tcctaaaaaa aagcatattc tttattcaga
    25981 tgaattagtt gatccaatca tacgatattt agaagaacat ctttcagaaa attttttgct
    26041 tgaacaatta gcccaaattt tttttatcag tccagccagc ttaaaaaaac attttagaaa
    26101 agtgaatgga aaaagtgtta ttagctattt taaagaacta aaaatggaaa aagcaaaacg
    26161 tctcgtgcta gaaaatgaat taacctatac agaaattgca gatatattag gatttcattc
    26221 gatccatcat ttttcttcag cttttaaaaa atatacaggg ttatcccctt caaagtatta
    26281 tctatcctta aaatagaaaa aaatttttag gttttatcca caaattattt ctctatattt
    26341 tctgtctatt caaaataaac aataattttc cggcacaatt atttgctgtt ttttaccata
    26401 acatagtacg atcaacgtag aaaaggagat gtttttatga aagttgttgt cattggcggt
    26461 gtagctggcg gtccttcatt tgccactcgt ttccgtcgat tgaatgaagc gcacgaaatc
    26521 attatctatg aacgcggaga aaatatttct tatgcaagtt gtgctttgcc ttattattta
    26581 ggaggtgtga tcactgaccg tgactcgctg atcgaacgta caccagaaat attgaaaaca
    26641 aaaaacaaca tcgatgtctt tactaaacac gaagtaacag caatcgatcc ttctactaag
    26701 cgattaacag ttaaagacct atccacaaat gaagaaataa aaacagatta cgataagttg
    26761 atcatctctt ctggtgctag accagattat ccggatattc ccggagtttt tgaagcagaa
    26821 aacggctttg tcctccgtag tgtgacggat gcggatcgaa tcaaatcgtt ccttgaagaa
    26881 aaaaatccac aacatgtcgt cattcttggt gcaggtgtta tgggtctgga attagctgag
    26941 aatttcaagc atcgcggctt agacgtgact ttaatcgatc aattgccaca agtcgctttc
    27001 ccttatgatc cagaaattgc taatttagtt tatgacaaat tgctgaaaga aggattagcc
    27061 gttcatttag aaacaagagt tactgagatc cgtgataaag gtcgagaaat agtattatca
    27121 gatggctccg tcctttctgc tgatatgcta atttttgctg ttggtgtttc tccgaagaat
    27181 gaagtggtga aagcaaccgg catacaatta tcagatacag gtcagatcat tgttgatgac
    27241 cagttacaaa ccaatctgcc ggacatctat gcgattggcg atattatcga aacgactagt
    27301 gtagtgactg gtcagccgat ccaaagtatg ctttccagtg cggccaatcg tcaaggacat
    27361 atgctggcag atattttaaa tggtacgcct atgcgctatc gcggatatat tggtgcaggt
    27421 gtcgcaaaaa tctttgatca tacagcaagt tatgcgggaa tgacagaaca tgaattaaaa
    27481 gcatcaggca taacaaatta taaaactgtt tttatcactc cctttgacca tgcctatttc
    27541 tatccagaag ctacaagatt aaatctaaag ctgatttttg atgcagatag cggtcgtatt
    27601 ttaggtggac aagcatttgg agaaaaaggt gtcgataaac ggatgggaga actttctgta
    27661 gcaatcaccg gaaacttgac agtctttgat ttgcctgatt tggagttgcc ttactctcca
    27721 ccgtattcta ctacccgtga cccgttgaat atagctggtt atgtcgcaat caatcaaatg
    27781 acgaatatcg tagaaacgat caaagcaagt gatatacccg aaaacgattt gaaagaagcg
    27841 ttctttttag atatacgtga acctaataaa gcaccttctg gcagtatctc ggcaacaaaa
    27901 aatattccaa tgaatgagtt acgagatcga atcaatgaaa tcccaaaaga taaaaaagtt
    27961 tacattactt tcagaagagg attgaatact tatacttctg cccgaatctt ggcaggtttg
    28021 ggtatcaaag cgattttgat tgaagaataa aaaaacgacc gcggtgggta gccgcggtct
    28081 aggagttatt tttacttgag gagtaaaaat gaaaaagtat atagggttgt tggttggtat
    28141 gtatatagaa tagaagataa atgtgaagaa attgtgcatt atttcttcac atttattagg
    28201 ttatagttta agaaagttta aacattgcgc aatttaatta ttttagctgt ttcttttcat
    28261 aaaaaaacat gttgtgactc tgagacaaat catagtccat agtgatgtct ctagagtaac
    28321 tacgcctaat attacgaggc atcatattaa tctatcagca ctagtaccta aactagcatc
    28381 cctttcgaat attttttctt atataataaa gtttgctttc actcctctaa tctcaccaaa
    28441 tcgactggct tgcgttgtgt gatactagga actgattggt ttagaacgac ttttctattg
    28501 taatcatatt tttcgccatc ctcgttgtct tctaatcctt caatgaatga tccctaatgt
    28561 gtgtattaaa taatttaatt tttttgttcc tatttattta aca
//
LOCUS       DACDUU010000031        28162 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.031, whole genome shotgun sequence.
ACCESSION   DACDUU010000031 DACDUU010000000
VERSION     DACDUU010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.031"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..368
                     /locus_tag="IWL30_001404"
     CDS             <1..368
                     /locus_tag="IWL30_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706179.1"
                     /translation="DFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDEYL
                     KENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNETIT
                     KTDMALMNREAEKIINKLT"
     gene            complement(404..706)
                     /locus_tag="IWL30_001405"
     CDS             complement(404..706)
                     /locus_tag="IWL30_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706180.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(955..1155)
                     /locus_tag="IWL30_001406"
     CDS             complement(955..1155)
                     /locus_tag="IWL30_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9706181.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(1460..2692)
                     /locus_tag="IWL30_001407"
     CDS             complement(1460..2692)
                     /locus_tag="IWL30_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP9706182.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2946..3515
                     /locus_tag="IWL30_001408"
     CDS             2946..3515
                     /locus_tag="IWL30_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9706183.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(3693..4133)
                     /locus_tag="IWL30_001409"
     CDS             complement(3693..4133)
                     /locus_tag="IWL30_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAP9706184.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            4291..5055
                     /gene="map"
                     /locus_tag="IWL30_001410"
     CDS             4291..5055
                     /gene="map"
                     /locus_tag="IWL30_001410"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAP9706185.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            5087..6010
                     /locus_tag="IWL30_001411"
     CDS             5087..6010
                     /locus_tag="IWL30_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAP9706186.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            6086..7225
                     /locus_tag="IWL30_001412"
     CDS             6086..7225
                     /locus_tag="IWL30_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAP9706187.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            7218..8018
                     /locus_tag="IWL30_001413"
     CDS             7218..8018
                     /locus_tag="IWL30_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9706188.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            8018..8845
                     /locus_tag="IWL30_001414"
     CDS             8018..8845
                     /locus_tag="IWL30_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9706189.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            8823..9557
                     /locus_tag="IWL30_001415"
     CDS             8823..9557
                     /locus_tag="IWL30_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9706190.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            9657..10523
                     /gene="rfbA"
                     /locus_tag="IWL30_001416"
     CDS             9657..10523
                     /gene="rfbA"
                     /locus_tag="IWL30_001416"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAP9706191.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            10537..11109
                     /gene="rfbC"
                     /locus_tag="IWL30_001417"
     CDS             10537..11109
                     /gene="rfbC"
                     /locus_tag="IWL30_001417"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAP9706192.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            11131..12159
                     /gene="rfbB"
                     /locus_tag="IWL30_001418"
     CDS             11131..12159
                     /gene="rfbB"
                     /locus_tag="IWL30_001418"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAP9706193.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            12257..13108
                     /gene="rfbD"
                     /locus_tag="IWL30_001419"
     CDS             12257..13108
                     /gene="rfbD"
                     /locus_tag="IWL30_001419"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAP9706194.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            13143..15176
                     /locus_tag="IWL30_001420"
     CDS             13143..15176
                     /locus_tag="IWL30_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706195.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            15220..16500
                     /locus_tag="IWL30_001421"
     CDS             15220..16500
                     /locus_tag="IWL30_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP9706196.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCRSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            16710..17516
                     /locus_tag="IWL30_001422"
     CDS             16710..17516
                     /locus_tag="IWL30_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9706197.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            17528..18748
                     /locus_tag="IWL30_001423"
     CDS             17528..18748
                     /locus_tag="IWL30_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9706198.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            18738..20324
                     /locus_tag="IWL30_001424"
     CDS             18738..20324
                     /locus_tag="IWL30_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9706199.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            20363..22501
                     /locus_tag="IWL30_001425"
     CDS             20363..22501
                     /locus_tag="IWL30_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9706200.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(22869..23852)
                     /locus_tag="IWL30_001426"
     CDS             complement(22869..23852)
                     /locus_tag="IWL30_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAP9706201.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            24230..25621
                     /locus_tag="IWL30_001427"
     CDS             24230..25621
                     /locus_tag="IWL30_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP9706202.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            25637..26677
                     /locus_tag="IWL30_001428"
     CDS             25637..26677
                     /locus_tag="IWL30_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9706203.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            26696..27781
                     /locus_tag="IWL30_001429"
     CDS             26696..27781
                     /locus_tag="IWL30_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP9706204.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            27814..>28162
                     /locus_tag="IWL30_001430"
     CDS             27814..>28162
                     /locus_tag="IWL30_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HAP9706205.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSV"
BASE COUNT         9095 a         4654 c         5652 g         8761 t
ORIGIN      
        1 ttgactttaa tgaatattta tctatttcca aacaaattga atcagatgat aaatataatt
       61 ctaaagaatc ttatagaaga actgctgttt caagagcata ctacagtgct tttaaaaaat
      121 ctgatgagta tttgaaagaa aattatgata ttatttataa tggaagtagc ggaaaaggaa
      181 gccaccaaac cgtttggaat ttgttttcaa cagttaagga attgaatacc ttaggaatac
      241 ataatagtgg gtatagaatg ttagagaaaa gaaaatgtgc tgactatatt cctaatgaaa
      301 ccattacgaa aacagatatg gcgctgatga atcgagaagc agaaaaaata ataaacaaac
      361 tcacctaatg aatttcaagc gagtttgttt gttatttttt tatttaatct ttgtatcttt
      421 ctggatcaac gaaagtatac tttatatagt cataacgccg atgatcgcta cgtgcgtccg
      481 gcacgtcagt cacgatatca aacaaaaagt atacaccctt cttcattcta gttttcgcag
      541 caggaatttt gaaatagttc ttattagaat agtagagatt gattaataag ctatcttcga
      601 ttgctaaaaa gaaaacttct gaattccata ctttataaaa atctttgata aatctattcg
      661 aagggtcaaa tttaaaccat aattgtgttt ttccttccat cagcataaaa gttcacctca
      721 aaaagagtat acgaacgaat gttctttttg tgaaggcgga atttaatata ctgaataata
      781 gaaaacaagt acttgtgcca aattatgtgc caaaaaaatt cgaattcaac aaaatgtaaa
      841 caaatagaaa accttctata ttaagatttc ctgcaataac aaacacaaca gaagacgtgt
      901 aatagttagt caggaacgta caaataaccc ctgtatcctt tgcggtacag ggggttattt
      961 tgtataagta gacattgtag tttctaaact gctatgtcct aaacgtttcg atacgctaag
     1021 tatatttact ccttgataaa gtaaaataga tgcatgcgta tgtcttagtc catcaataaa
     1081 cagtcgaaga gaaaataggg aaaaattgat tactaaagaa aaacttacta ggtcgacggt
     1141 gtagttgatt ttcatacttc aatagattcg aattaaacga tttcgattgg cttataccag
     1201 tcctatccaa cgaaagatat atcgttctta actctagcgg tataacttga agctttacgt
     1261 aaagccaatg ggaatgaact ggagatgact ttttattatt tttagtaata gctgatgggt
     1321 taatacttac aactttcctt ttcaaagtac catataaaaa aacggtgggg ttgtttagcg
     1381 aaataaagga tctaagccaa tagcttagat cctttattct atgctttata ttattttttg
     1441 catgtgtgcg aaagtatact caagcccaat cgccatttcg gaaaatcggt actgtagagc
     1501 catcttcacg gattccgtcg atatccatct tgtcagatcc taccatgaaa tctacgtggg
     1561 tttgacttct attcaatcca gcttctgcta attcttcatc ggacatttct gtaccgcctt
     1621 tcacactgaa ggcataagca gaacctagtg ctaagtgatt cgatgcgttt tcatcaaata
     1681 atgtattgaa aaagatgatg ccagattgag aaataggtga agggtcaggt acaagtgcga
     1741 cttctcctaa acgacgcgca ccttcatctg tatcaagcag ttttgctaag acatcttctc
     1801 cttgttcggc agaaaaatca acaacttttc catctttaaa agtaaatttc atacccgaga
     1861 tgatggtgcc agcataactc agtggctttg tacttgaaat gtaaccatct actcgatgac
     1921 tgtcaggtgc tgtgaacact tcttccgtcg gcatatttgc catgaatttt tctccacgag
     1981 cattatagct tccagcgcct tcccaaagat ggtttttagg caatccgata atgatatctg
     2041 taccgggagc agtatagtga agggcagaaa attgttctcg attcaattct tctgcttttt
     2101 ttgctaattt ttcatcatgc ttcttccatg ctaagacagg gtcttcttca tagacacgag
     2161 tcgttttgaa aatctgatcc cataatgcct caacttgttt ttcttctggc agttctggga
     2221 agacttttgc tgcccattgt ttaccagccg cagccacgac agtccaactg actttatttg
     2281 cttgagttgc cttgcgtaaa ttcattagtg ctttgccatt cgctgattga taagacgcta
     2341 ctcgatggct atctacacca gcaaatgcat cgggatcggc agaaacaacg ctgatcctgc
     2401 ttgctccttt ttcaagccat tcatccgctt gatcgatttt agattgtggt acattctcaa
     2461 tacgatccgt tgcagcatgt aaaagaaatt ctctttggat ctggtcgtca gtccattgaa
     2521 cgataacctc agcggctcct agtttataag cttcttgtgt gatcaatcga gcaaggggtg
     2581 cctgttccac gctgatctgt aaaacaactg tgtgtccttt ttctgtggct acgccagttt
     2641 ctgcaattaa gcgtgcatat ttttttagaa gttcattaaa atcagataac ataataaaaa
     2701 ttcctcctca ccattttttc tggtaactat tagcatgata ccatttaatt atgattcaaa
     2761 caatgaggct ttaaataaaa aaaggaggtt atttttttaa ggttaagttt catgatatat
     2821 gttactactt gctatttcag cttgtgaaga aaatcaaaaa ctaattttgc atatcaaaaa
     2881 aacatgttac aatgaaatta atcaatggtg gaagcgttat aaaaaaatta aggagattaa
     2941 aaaatatggc aagaaaaaag acgattacta gagaacagat tttggcagct gcttatgaag
     3001 tagctgccac cgatggattt acacgattta ctgcgagaaa catcgcgaac aaaatgaatt
     3061 gttccacgca accgatttat cttgagttta aaaatatgga tgacttgaga caagctcttt
     3121 ttgagcaaat ctatgatcat ttacaatatg aagtgttcac tgttgagcat acagggaata
     3181 cgattatcga tcttgctttt aattatatcc attttgcaag aagagagaag aagttgtatc
     3241 gttcactgta cttagaagaa tatggtggag gaaaagagat gcagaatttc tcttatactt
     3301 actttgtgaa tacagtgaaa cgcgatccag cttatgtgaa tctttcagaa gagcagatcg
     3361 aatctttgta taatgggaca tggatcatag ctactgggat cgcagctttg atgtcttcaa
     3421 gcatcatcca tccaactgat gaacagattg aaaagatgat ccaagacagt atagatgcta
     3481 tcttgaaatt caaagaacca atcgaaatcg attgaatata ctcaaaagtt ccacaaatca
     3541 gtaggtaaca agagatgtgt caaaataagg aaaagttcgt ttttcttaca gcgtatgcat
     3601 ttttaactcc cgaagaactc ttccatgaat gcaatgaata aaagagccat acagattgaa
     3661 taaatctgga tggctctttt attgcaagta tattaaattt ttgcgactag ttctttagcg
     3721 aaagcttcta aattttcgat atcttcttct tcagcattca gatccacttt aacattttcg
     3781 gctccttttg atgcgcctgt ttttgcaaaa gcagcatcga aatcatccac cgatttgcag
     3841 aattcatcgt aaaaagtatc accagatccg cagacaccgt aaatttttcc caatagatcc
     3901 aattcttgca agtcttcata aaagtctacg atctcatcag gcagatcgcc atcatcgtat
     3961 gtataagtag ctactacgca aatatctgcc tcctcgaatt catctgcatc tacttgggta
     4021 cattcgttga tttcgacttc gatatccaga ttttcaaatg cttctgcaac aatatcggcg
     4081 atttcttcag tgtttcctgt catgctagca tatacgattt ttgctaaagc catgatgtgc
     4141 ctcctcgttt ataattattt aacaattatg aattatacca aaacatcatt gaaaagaaaa
     4201 taaaacctaa agacacctgg ggaatgtcat ttggctaaat gtatgccgct atgttaaaat
     4261 gagagggatt tgtaaaaagg agacgaaaat atgattactt taaaatcagc acgtgaaatt
     4321 gaaatgatgg cagaatctgg agctttatta gcagatgtac ataaaaatct gcgtgatttt
     4381 ataaaaccag gaatcacaag ttgggatatt gaggtatttg tcagaaacta tatcgagagt
     4441 catggcggaa ttgctgcgca aattggcttt gaagggtatg aatatgcgac ttgtatcagt
     4501 ataaatgatg aaatttgtca tggattccct cgtaaaaagc ctttgaaaaa cggtgatttg
     4561 gtaaaagtcg atatgtgtat cgatttgaaa ggcggcattt ctgattcttg ttgggcctat
     4621 gtagttggag aatctacacc tgagatcgac cgattgatgg aagtaacgaa gaaagctttg
     4681 tacatcggta ttgagcaagc acaagtaggg aatcggattg gtgacatcgg tcatgctatc
     4741 caaacttatg tagaaggcga aaatctagcg atcgttcgtg attttattgg tcatggcgtt
     4801 ggtcctacga ttcatgaaga accagcagtt cctcattacg gagaagctgg aaaagggttg
     4861 cggttgaaag aaggcatggt tatcacgatc gaacctatgg ttaataccgg aacttggaaa
     4921 atgaaaatgg atcctaacgg ctggactgcg tatacaagag acggcggact ttcttgccaa
     4981 tatgaacata cattggctat tactaaagat ggtccaagaa ttttgacatc acaaggagaa
     5041 gaaggcactt attgatctat ccagtcaata ggtacgggag aggtacatga aagtttggaa
     5101 caaaataaaa caaaatgaaa atctgatgcg ctttattgag acgactcaaa agcgcatcac
     5161 tgattcagaa atgagtacga cctctgtcgt cgttgcttat tatttactct tgtctttatt
     5221 tccattgatt attgcttttg gtaatatttt accgtttctc cacatcgatc aagagactgt
     5281 actgacctat ttacgagaag tgattcctga gaccgtttac cagtttatcg gtcctgcaat
     5341 caaagacctt ttgacacaaa gttcaggagg cttgttatcc gtttctgcat tagcggcttt
     5401 atggtctgca agtcaaagta tcaatgcttt gcaaattgcg atgaataaag cgtatggcgt
     5461 agaaaataga aaaaattttt tgatcgtccg tttcttctca ctaattgtca ttgtcttgtt
     5521 catgattgct attagtgggg taactatcgt tcttgggttg ggacagctga ttttggatgc
     5581 tattcagcca atcgtacaaa tacccgtgaa ctttatcgat cagttccaaa ctttgaagtg
     5641 gccaatcaca ttggtagctt tatttgtcat catgttcttg atttatttaa ttgtccctaa
     5701 tgctcagttg aagttgaaga cgatcattcc gggcacgatc ttcgctactg tcggctggat
     5761 gctgttatca caagtgttta gtatctatgc taggtatttt gcaacgagga tcagcggtta
     5821 tcagatcata ggaagtttta ttgtactcat gctttggctt aattttgctg ctacgatcat
     5881 cattttgggc ggaatcatca atgcagttgt tcaagaatac gtgacgggtc atcaaatcaa
     5941 aaaacgtcga gatccaagcc gaaaatggtt caataaattg aaaactaaat tttctcataa
     6001 aaaaaagtag aaacaagagt tcttattgta aatttatatc agtaatttat aataattatg
     6061 actcttaatt aaagtaggtg cccatatgct tgtttctttt agtatggtta taagattttt
     6121 tctgacaatt ttattatcac tgatattaac acctgtattc aaaatcattt cagtacaaac
     6181 aggtatggtc gacaaaccaa atgaaagaag aataaataaa gttccaatgc cttcagcggg
     6241 cggactgcct atctttattt catttgttat atcgacttta ttcttattcc gtaaaattat
     6301 ccctcaatcg tatattgtac cgatcttgat tgcttcttta gtaatcatag tcacaggagt
     6361 gctcgatgat aaatatgagc tgacacctaa acaaaaaagt atagggatct tgattgcttc
     6421 tcttattatt tattttgtag cagatatacg tattgattcc ttcacgcttc cttttattgg
     6481 atatatccaa ctaggctggc ttagttttcc cgtaacgatt ttttggatct tcggtatcac
     6541 gaatgcggtc aatctgattg acggtttgga tgggctggcg gcagggattt cattgatcgg
     6601 tttaattacg ataggaatca ttggatattt tttcctccat gcttctacag tgtatattcc
     6661 agttgttatt ttctgtcttg tagcaagtat tatcggtttt tttccttaca atttttatcc
     6721 ggcaaagatt tatttaggcg atacaggagc tctattccta gggtttatga tggccgtcct
     6781 ttcactacaa gggctgaaga acgtgacttt tgtttcatcg atttctttgc tgatcgtcat
     6841 gggagtgccg gtaacagaca cgttttttgc tatcattagg agaaaagcaa accgtgtttc
     6901 tttttctaca gcagataaaa aacatcttca tcaccgacta atcagtttag gctttactca
     6961 taaaggtgct gtcctgacga tttatgcgat ggctttgatg ttttctttca cagctatggt
     7021 catgaattat actggaagaa tcggaacgat catattgatt attgccatgt tgtttgctgc
     7081 aattttatta tttgaattga ttggtctgat caatgagaaa catcagttta ttttaaaaac
     7141 attgcgattt ttagggaata aagagtaccg tacgcaagta aaacagaaat atttgaagaa
     7201 atggaaccgt catatagatg aataacaaat taaaccagcc gatccttatt agtattgtga
     7261 cggcaaacag tgataaaatt tttcggacac tagataattt tatttcaact gtaaaagatg
     7321 aaacagcagt aaaaatccga atttttgaca acaattcact tcccgagtac aaaaaaagac
     7381 taacagagta taggaaatat ccctacatcg atatctattt tcatgatgaa aattccggat
     7441 ttggttatgg acataatcac aatctgatag ctagcgattc tcgctatggc attatttgta
     7501 acccagatat tttagtggat gaagagacag ttacagcatt ggtcttgtta ttgaaagaac
     7561 atccggaatg tgcgatgtta gcacctaaga ttcttaatga agatggaaca actcagcacc
     7621 tcatccgtga gcagctggct gtttttgatt acgttttgcg ctttgtgcct ttcaaatggg
     7681 tcaaaaaaaa gtttgacaaa cgactggctg tgtttgaatg ccgaggattg cctactgatg
     7741 ctaatagtta tgtacggatg atctcaggta gttttatggt agctgatatt gataaattta
     7801 aagagataaa ggggtttgat gagcgtttct tcatgtactt tgaagacaac gacttgtgtt
     7861 tgagttttga aaaagcaggt tacaagttat tgtacacacc cttttatgca gttatccatt
     7921 tttacgggaa gggtgcgcat cgaaatgcaa aactgtttcg cgtgtttttg acttctatga
     7981 tgaaattttt caataagtgg gggtggcgtt ttttctgatg aaaccagaaa tattagtagt
     8041 cgttgtgctt tatcagcaac tattcagtca atctccagct tatggaccat taagcaaagc
     8101 acttagcgaa aagcaaattc agcttattat ttacgataat agtcctcaaa ggcagcaaaa
     8161 tgagctgttt gaaaaagaaa atatcgttta tcatcatgat ccgcaaaatc ctggtttagc
     8221 ggtggcttat aattttgcgt tgtctcaagc agatgaagga acacgttgtc tcgtcactct
     8281 ggatcaggat acgaggctag tcgacgatta ttttgaacta ttgcgtaaag taacctttac
     8341 agatgaatgt gttgctgcag ttccgatgat tttttctggg agccgacaag tttctcccct
     8401 atattcagat cgttatatca atcgtacagc tcaagcagta gaagttgaag gcccaacagc
     8461 tgaacgaatc atggcaatca attcaggtgt tgcctggtct gtcaaatttc taaaaaagat
     8521 tggcgggttt aatcctgctt tttctttaga ttttttggat cattggctgt tttggaaagt
     8581 ggatcagttg gaaaaaactg tggagatcct tcctgtacga atggaacatg atctttcggt
     8641 tttagactat gaaaaagttt caagtaatcg ataccgttct attttacaag cagaaaatcg
     8701 attttatcat gaatatgatc aaaggcattt atccagtcac cgacgacaat tattgttaag
     8761 aacagctaaa cagttcttga ccgtcaaaaa ccggcatatc tggcggatga ctttgcgctc
     8821 atatgttaag aattggaagg tgtgaattcg tgcgttcggt ttgtatagct acctttaatg
     8881 gaggcagata tttaagagag cagttagata gtattttatc tcagctttca gcagaagatg
     8941 aagtagtcat ttctgatgat gggtcagtag atgatacatg ggatatatta actgaatacg
     9001 cagaaaaaga taagcgaatc cagctttttt cggggcctcg tcaaggattg atcgctaatt
     9061 ttggttatgc gatccagcag acgaaaggag atctaatctt tttggcagac caagatgatg
     9121 tttggttgga gacaaaagtg aaaaagatca ctgactattt tgagcagaat ccggatcagc
     9181 tagtagtagt ttctgattta atcattgtgg acagcgagct gcaagagatc catgattcgt
     9241 atttttcgtt tcgaaattcc aaagaaggat ggcttaataa tgtgataaga agtcactata
     9301 ttggtgctgg tatggctttc cggtcttctt taaaacccga tattttgccg atcccgtcag
     9361 aagtgccgat gcatgatatg tggattggtt tgctgggagg aaagaagact gggttcctaa
     9421 gagaaccgtt aactctgtac cgaagacatg atttaaacgc gagtgagatt gaaacacatt
     9481 ctaaattacg gcaaaaaatt tcttggcgtg ttcatctgat tacggcgttg atcaaacgaa
     9541 aatggtttaa acgttgataa aagatgttta aatccttaat atttccatga caaatataac
     9601 aattgatgag ataataacaa agtgaaattt gaaatcaaaa aggagagcag atatccatga
     9661 agggaatcat tcttgctggg ggcagcggaa cacgtctgta ccctttaaca aaggcaacat
     9721 cgaaacaatt gatgccgatt tatgacaaac caatgattta ttatccaatg tctactctga
     9781 tgttggcggg aatcaaagaa attctgatta tttcaacgcc acaagataca ccacgtttca
     9841 aggaattatt tggaaacgga aatgatttag gactgcagat cgaatatgca gtacaagaaa
     9901 gtccagatgg attggcacaa gcgtttatca ttggagaaga atttattgga gatgatagtg
     9961 tctgcttggt attaggggac aatatttact atggtggcgg tctgtctaaa atgttgcaac
    10021 gagcagcttc aaaagataca ggcgcgacag tatttggcta tcatgtcaat gatccagaac
    10081 gctttggtgt agtagaattt gatgagaata tgcaagcttt gtcgattgaa gaaaagcctg
    10141 ctcagccaaa atcaaattac gctgtaactg gcctttattt ttatgacaat gaggtaattt
    10201 ctatcgctaa aggtatcaaa ccttcagaac gtggagaact agagattacg gatgtcaata
    10261 aagcatattt ggataaagga aaactatccg tagaacttat gggacgcgga tttgcttggc
    10321 ttgatacagg aacacatgaa tcattgcttg aagcatctac ctttatcgaa acaattgaaa
    10381 aacgtcagaa cttaaaagtg gcttgtttgg aagaaattgc ttaccgtatg ggctacatcg
    10441 ataaggaaca attgctggcc cttgcacagc cattgaaaaa aaatcaatac ggtcaatatt
    10501 tattgaattt ggccgctgaa taaggagtcc ttacagatga aagtaacaga tacaaaatta
    10561 acagatgtca aaatcgtaga aatggacgtc tttggcgatc atcgcggttt cttcacagaa
    10621 agttattcaa aagcaaaatt tgcagaacat ggattgaaat atgactttgt tcaagacaac
    10681 cattcactat cagctgaagc tggtgtcctt cgggggcttc atttccaaaa aggagaagct
    10741 gcacagacaa aattgattcg ggtagtcact ggagcagttt tagatgtagt agtggatatc
    10801 cgcaaaggta gcccgactta tggtcaatgg gacggattta tcttatctga acataatcat
    10861 cgacagttgc ttgtacctaa aggatatgcc catggattcg ttactttgac accaaatgtg
    10921 aacttccttt ataagtgtga taattactat aatgcagaag cagatggggg catcgcattt
    10981 gatgatccag atttagcgat tgattggcca attgatccat caagagcgat tatgtcggaa
    11041 aaagatcacc gtcatccgac attaaaagaa tttgaagcag aaaatccatt tgtttacgga
    11101 gaaatctaag aattaaggag aaattttctt atgaaaaaca tcattgttac cggaggtgcc
    11161 ggattcatcg gctctaattt cgttcattac gtggtaaaca atcatccaga agtgcatgtc
    11221 actgttttgg ataaactgac ttatgcagga aacaaagaga acttggccgg acttcctagc
    11281 gatcgcgtag aattggtcgt aggagatgtt gcagatgcag aattagtgga ccgtctagta
    11341 aaagaagcgg acgcagttgt tcattacgcg gcagaatccc ataacgataa ttcattaaaa
    11401 gatcctttcc catttgtaca aacgaatatc attggtacgt atacactttt agaagcttgc
    11461 cgcaagtatg atgtacgcta tcatcacgta tctactgacg aggtttacgg cgatcttcca
    11521 ttacgtgaag acctgccagg acatggggaa ggcgaaggag aaaaattcac tgcggaaaca
    11581 ccatataatc cttcaagtcc atattcttct acaaaagctg gttcagatct gttagtaaaa
    11641 gcatgggtgc gttcttttgg cttacgtgca accatttcaa actgttcaaa caactatggt
    11701 ccttatcagc atattgaaaa atttattcct cgccaagtaa cgagtatctt aagcggtatt
    11761 cgtccaaaat tatatggcga aggaaagaat gttcgtgact ggatccatac gaatgatcat
    11821 tcttcagcag tgtggctgat tttgacgaaa ggcagaattg gcgaaaccta tctgatcggt
    11881 gcagacggtg aagaagataa taagaccgtg atggaattga tcttagaaat gatgggccaa
    11941 ccaaaagacg catacgacca tgtaaacgac cgcgcagggc acgatttgcg ttatgcaatc
    12001 gattcgacaa aattgcgaga agagctaggt tggcagcctg agtttacgaa cttccgtgat
    12061 gggttggctg acacgatcaa atggtacgaa gaacatgaag actggtggaa aaaagaaaaa
    12121 gaggccgttg aagcagcata tgccaaaaat ggacaataat acagaggaaa tttcactgta
    12181 ttaatggaaa aacacatgtg aggcaggcgc tcgcctgtct cttttttaaa ataaaaatca
    12241 aggagcggat aaccatatgt ttttattaac tggaggaaat ggacaattag gtacagaatt
    12301 gcgccattta ttagatgaaa aaaatctgcc atatgtttca acagatgcaa aagacttgga
    12361 tatcacagat gctgagaaaa caatggctta tattacagaa ttaaaacccg atgttatttt
    12421 ccattgcgca gcttacacag cagtagataa agcagaagaa gaagcaaaag aattagatga
    12481 gaaaatcaat gtcgatggca caagaaatgt agcacttgca gcaaaagctg tgaatgcaca
    12541 attcgtctat atcagtacag actatgtttt cgatggaaaa aacaaaaatg tagaatacga
    12601 agtagacgac caaacgaatc cactaaatga atatggtcgt acgaaattat taggcgaaaa
    12661 agcagtagaa gagattttag aagactatta tatcatacga acttcttggg ttttcgggat
    12721 ctatggacac aatttcgtct tcacgatgca acgcctagcg gaaacccgcg acaaactgac
    12781 tgtagtaaat gaccagtttg ggcgaccaac ttggacacgt actttggcag aatttatggt
    12841 atatgtcatc gaagaaaaag caccatttgg tatttatcat ttgtcgaacg aaaatagctg
    12901 ttcttggtat gagttcgcaa aagaaatatt gaaagataca gatgtagaag ttgcacctgt
    12961 tacgtcagaa gaatttccgc aaaaagcaac acgtccgcag tattcagtca tgagcctgaa
    13021 aaagacagaa gcgttaggtt ttgtcatacc aacttggcaa gaagcacttg cgcaaatgct
    13081 ggaagctgcc aaaaaaatga ataagtaatt tcctttttat caaatagaat ttggagcatg
    13141 atatggaaaa acaaacaaga agtgcacgac gaaaagaaaa gccaaaagcg aatcaggcag
    13201 attcgaattt ggtgcttgac ctgtttctaa aactccgcta tcttattggc gttttagtca
    13261 tcgtggtcat cgttactttt aatttaaacg gaagttcact tggtgtttgg gataaatatg
    13321 tgtcgcaaag agacgatggg aaaaaaactg atgtgatttt tggacagaat cgggaagtgc
    13381 ggtcagacga atggctggtg cagacacctt tttacctctc acaagctgaa gaggactttt
    13441 cgttggtgaa cgaggattat tcattaaacg gacaaaacat gatcattgcg tataattctc
    13501 ctgtaaagga tattacggtt atcggaaaac cattcaactg gggattcttt ttcctcggaa
    13561 gagatcgagg actttccttt tattgggcga tgaaattagt cgtcatggtc ttgcttggat
    13621 tcgaagtact gatgatatta acgaagagga aaaaagctct atcgctaatc ggtgcatttg
    13681 cgctgacatt ttctccagca gtccagtggt ggttcatgca acatgtgggc gatttgattt
    13741 tctttacttt gggactgatg attgcttttt atcattattt ctacaatcat gataaaaaat
    13801 ggctacgaac gttgatgatg cttttggtgg ttattttcgg tattgggttt attttagtga
    13861 tttacccagc ccatcaagtg atgctggcgt acttgctgat tttttatttt gttggcctgc
    13921 ttatttacta tagcaagaaa gtttcatggg atctgttgga tgtagtgttg attggagggg
    13981 cagttttatt tattggcgga gtcatggttc atttctggct aacgtcaaaa gacgcattgt
    14041 tagcttcttt gaatacctta tatccaggaa aacgggtaag tactggagga aagtggacga
    14101 ttggcgaatt tttttctttc cttacaaatt ggaaaattcc ttttgaagat atcaactatt
    14161 cgaataactc tgaagtggcc ctgttctatc acttttttcc aactgtattc ctagcaagtc
    14221 catttgtgtt atttggcaaa aaagatagcg aacaaaagtt actgggacgt attttgatga
    14281 ttttttgtct gttttctatc ttttggatta ccgttgggtt gccaaaatgg ctagcagaaa
    14341 tcactctgct gtcttatgtt cctccattaa gagccatcct tacttttagt tttgcggctt
    14401 gtttattatc gatctggttt atcgcttata tttggagaga agatgttatt cctatttggt
    14461 ataaattcat tttgttggca gtaaatgcat ggtcttattt ctatgcgatt acgcattcga
    14521 aaatggataa ttatttttcg gattttgaaa ccatcgcagt tgctgtgttg ggaacagtga
    14581 ttttagcttt tgcattattt ggctggagac gcttgtttta cctcgctttt gtcgccttag
    14641 ttatcgtatc tggctttttc gtcaatccag tcgtagaagg tacaggagcg atttttgaaa
    14701 aaacactggc aaaagaaatc caaaaaatcg atcaacaaga tcctggtaaa gtttggttgt
    14761 ctgaagatga attatataac ttcaccccaa cattgggagt gaaatccttc aatacagtac
    14821 gcttttatcc agatatagct gcatggaaga aaatcgatcc gaaagaagaa tacgagaagt
    14881 attataatcg gtatgctcat acaagagctt ttatcgctaa ggaagataca aagttcattt
    14941 tagatcgtcc agatatgttt tcggtcacgt tgaattttga agacgcagaa aaattaggga
    15001 tcgactatgt gatcagtaaa cgaccattaa ctgattacaa ccaattatat gaagcgcagt
    15061 tcacgcagct atatggaccg gataaagacg gttatatgat ttttaaagta gaatatccgg
    15121 attatccaga tgtaccacaa acacaaactc aggaaaatca gctagtcgta cagtaatagg
    15181 ctagctgaga ataatcctga aatattagta gaaaggagag tgaagataat tcaagcaatc
    15241 gtacaaaaat ttgtcaacaa aatgagtgtc ggtttcttgc ttttgttgct gacgctagtc
    15301 ttttttgttt ggacagcagg ggtaggtttt ccagcaactt tgtttatata taacaatagt
    15361 tcattactta tcgcttttag tgtggctatt ttgctcattt taaatgttta taaagcaaaa
    15421 ggcgtcgatt ttctttatct aggtgttgcg cttgcccttt tcattttctt ttcttacttt
    15481 cgggaattaa gaaatgagtc tttatttgga gacttactgt ttcctttgat tattttattt
    15541 tgtttagtaa ttaaatggat cgagtggaca aaatgggatc gcggtattta tctgattgtc
    15601 tttacggtct tcctcggaat cacgttatat cgtgtattta cagagatcca agtaccagaa
    15661 ggacaaagca tctgggctcc aggcaattcg ctttctgata tttggatcaa tacgaataca
    15721 atcggaagtt ctttgatgac tcttgcttta ttgatcagtg gatttgccag ttcttttgaa
    15781 cgatggtatg ttcgaatcat cggtgggata gcagtcgttg cagccttttt aggtatttgg
    15841 gtctgccgat ctagaggtgc attagtcgcg ttgattgttt ttgcgatatt ggatctttta
    15901 cctaaatcgt tgatgcgtgt cattcgtgcg ccatttattg cctacacggt tacgattctt
    15961 ttagctttgc cgatttctta tctggcggct gtctctgaaa aagtcaattt gtttaccggt
    16021 cgagaagata tttggcataa attctatcag acgctaggag aaaaatctga acaagtttta
    16081 ctaggaatga aaacatttat cttccagcga ggaaaccaat ttttaggaaa ccacaatagt
    16141 tataattcta ttttgaatat ctatggattg ataggatttg ggattgctgc tttgctgctt
    16201 atccttttta ttggtcgctt aacgttgaaa gcagatttat ccaacgggca gatgactttt
    16261 atctgggcct tttttgcggt gatgatgcag tcatttatgg aggatacatt gacttcatac
    16321 ccatgggttc cgattattta tatcctgttg gctatggccg ttcatcgata tgatgaacca
    16381 gaagttacgg aggaattcta tgttatggaa gaggcatatg acgatatgac ttatgatgat
    16441 acatcttatg aggaacaacc agatagcgct cattcttcca ggatccaacg ctatcattga
    16501 taggcacaaa atacgagaga ggctgtgaca accgtcgcag ccttttttgg tttattgcaa
    16561 ttgtctagca tcttctgtaa aatagaaacg ttagagaaaa tcttgtatca tagaaaatta
    16621 agaaagaaat aagaacgcag ctcggtacca aaaatatggt aaaatggcac ttgactgtaa
    16681 ttcactgaaa atcgcttgga ggaacacaaa tgtttaaaga tttgtattta tttatgaagg
    16741 acatttatca gaacagaaaa ttactgctac agttttcgat caatgacttc aaatcaagat
    16801 atgcaggatc gtttttagga atcttatggg cgtttatcaa tccgctattt actgtattga
    16861 tttattggct ggtatttggt gttggactga aagcacaaat gatcgatgca aaatacccat
    16921 ttatcgtata tttagttacc gggatgattc cctggttctt cttctcagat gcatttgcat
    16981 caactacggt tgtttttcgg gaatatactt atttagtaaa gaaagtcgta ttcaatattc
    17041 gtatcttgcc cacgacgaaa atcctatcca atctgtacac ccatttattc ttcattctga
    17101 ttggcatagc tgtcgcgctt ataaatggta tatatccttc agcaatgtct ttacagctga
    17161 tttattattt attttgcatg tgtgctttct tgacaggatt gacttggctg acggcatcga
    17221 tccaaccatt tttacctgat atcatgcaat taattacgat cgtgctgcag ttgatcatgt
    17281 ggacattacc gatattatgg agccctaatc agtttgattc aggtcttgta agcatattga
    17341 aattaaatcc gctgtactat gttgttcaag gttatcgtga gtcattttta agtgaagctt
    17401 ggttctggga gcatggaaca tatactttgt atttctgggt tttcacgtta gtaatgttcg
    17461 taatcggctc tgcagtgttc cgtagattga aaccacattt ttcagatgtg ctgtaaggag
    17521 aatagctatg acagaatatg caattagact aaaagatatc acaaaatctt ataatatgta
    17581 cgcaaaacct tcggatcgat ttcgtgaagc cctcaatcct tttaagaaat cctatcatga
    17641 tgtattttat gcgttgaaaa acgtgaatgt agaaataaaa aaaggtgaga tgatcggttt
    17701 cgtgggagaa aacggatcgg gaaaatctac aatgttgaaa atcattacgg gagtactgac
    17761 accaacatcc ggcacgatgg aaatcgaagg aaaaatctca gctttgctag aactaggatc
    17821 aggattcaat cctgagtatt ctggctatga aaatatctat ttaaatggaa tggttctagg
    17881 attttcaaga gaagaagtcg atgaaatggt cgatgatatc attgaatttg ctgacatcgg
    17941 cgaccatatt tatcaacctg taaaaaccta ttcaagtggg atgttcgttc gtctagcttt
    18001 tgctgtagcg atcaatgtga atcctgatat tttgattgtg gatgaagcat tggcagtagg
    18061 agatcttgaa tttcaactaa aatgtatgga aaaatttaca gaaatcaaga actcgggaaa
    18121 gacgatttta ttcgtttctc atgatattag ttcgattcgc cgtttttgtg atcgttccta
    18181 ttggttgaaa aatggaaaag ttgtcgaata tggtgatacg atggaagtaa cgacgaatta
    18241 cgaaaacttt ttgaagaaaa aatcagttaa aacagtagat cgaaataaaa accaagtaga
    18301 acgcttcgca gctccagata tcgtagacgt gacgaaagcc gaattgttgg ataaagataa
    18361 aaatccagtg gatatgctgg aacaaggaga aaaactatac gttaaagtaa cctatgaagt
    18421 aaaagacgat acaatcaaaa atccagtttt aggaattgct ctgcgtacag tcgacaattt
    18481 ttatgtgtgc ggactcaata ctctactaga tggtatcaag atcccttgga aaaaaggtga
    18541 gaatatcttt tatctggagt atgaaaagtt aggtttgtta gctggtgaat attattttga
    18601 cgttgcggtc tttgaagaaa atgcaacggt tcctttggta tacaagacaa aatacatgaa
    18661 cttgtttgtc agcggttctt atatcggaga aggcatcgtg gtcttagacc ataaatggga
    18721 agaagggact catagcaatg aaatatgatt ttgaaatgga cctagatgaa caaagcagtg
    18781 tgggaaaaat tgccgcacag atcaaaccag gaagtaaagt tttagagttt ggtcctggaa
    18841 acggccgatt aacgaaacat ttgatcggag caaaacaatg tgaagtgagc attgtcgagc
    18901 tagataagga attgttcgat tttgtcagcg agtttgccca agatggattt tatggagata
    18961 tcgaaagttt tgaatgggca aactattatg ctggacaaac atttgattac gttttgtttg
    19021 ccgatgtttt agaacatttg gtagatcctg gaaaaacgtt gaagaaagtt agagaatttt
    19081 tgaatgaaga aggagaaatt ctaattacat tccctaacct tgcgcataat tctgtcatga
    19141 ttgatttgtt caataatcaa ttgccatggg cttcttatgg tttgctagat gaaacccata
    19201 attcttttta tactcatgat ggcttccaaa aagtttttga aaaagcagga ttatacatca
    19261 atattgaaga ttatttgtac ttagctgtag gtgatacaga attaaaatca acttatgaag
    19321 aattgccgga agctgtacgt tatgatttca aaatgcgacc atttggcgaa gtctaccaat
    19381 acttcttctc attgatgaaa catccagtag cacaaagttc cattgcgcag ccgcaaaatt
    19441 ctaattatgt gaaagtatta gaagtaatcc agcaaacgaa acaagatgaa ataattcaac
    19501 aaataccatt caataatttc acaggggaaa atgagacact aagtttccat gtatcagaat
    19561 tagcagaacg aatggtgttt cgcttcgcgc agcaacctag ctttatcgaa ttcagcgcag
    19621 aagctgctgg agaaaaactc acatttatcg attcaaatgc tgtagtcaaa acagtaaatg
    19681 attgttattt gtttgatggt aaagatttac cagagttctt tttaacggat attgcaggga
    19741 aagaagtgac tatccattgt cactatcgtt ttatcggaga attgacacca acaatgaaag
    19801 aattgctgga aacaatccgt ccaatggctg aagttacaaa gcagctttct gagaaaaatg
    19861 atgaattaga aagagaaaat cattatctta ttgagaataa tacaagatta gatcagacgt
    19921 tgaaaacaac cataaatcgc tattgcacgt tgttggaaag tgatgaatgg acaatcaaac
    19981 atagattagg gcgacgtaag aaagaaacat cgaaaaaaat ccaagaaaaa gagctttctc
    20041 tatgtattga cgaaaaaatc tgggatgcag aaacaaagat attgaaaatc atcgggtggg
    20101 gaatcgctaa ttcggatcgc caaccattgt cctataagtt atcagccgac caatcaccat
    20161 tttttgaagc aattcctgtt ttacgagaag aagtcaatca agcaaaacag ctagctgaag
    20221 gagcaaaagc aggatttgaa ctgcggatcc tttgcgagaa ggaacgttca ttcttagttg
    20281 aagcagtggc tcaaaatggt caatcatgga tcattgaaat gtaaataatg attaacttat
    20341 aaagataagg agtaaacgaa tcgtgcaaaa aaaagaaatt gccgtattta tcgatcaaat
    20401 cactcgtgaa cgggccactg gcgatttgct tattgtgggc tgggcaattg atgaagtgac
    20461 caaagaaatc cctacgataa aggtagaaaa agaaaatgtg attgctgaag caacacatgt
    20521 cgtaagattg gatatcaatc atttatataa tcttgatgtt aagacccaaa gcggttttaa
    20581 ggtacgttta tcagggaaaa tgagaggaaa agcaatctta gacttccaaa cagcaagaca
    20641 tcaaaatggc atagctgtga aattaaacgg taaatatcct tatgatgacg gcattgaatc
    20701 ttcatgggaa agaaaaaaaa gattactgaa aaaagggata aattatgcaa ggactcatgg
    20761 cgtaaaaaaa gcaatccgcc gggtcaaact ggaattgaaa ccaggatcga tcgactatgc
    20821 agagtggatc agtcgtcatg agaaacctga tctgaaagag caaaggaaag aagtacaagg
    20881 atttgcctac cgcccattga tttccatact tatgcctgtc tataacgttg aaatcaaatg
    20941 gctcgaaaaa tgcattgatt cggtacttga tcaaacgtat gatcactggg aactatgtat
    21001 cagtgatgat gcttctactg atccagctat ccgaaagtgt ctagaatcct atcaagcaaa
    21061 agatgataga attaaagtcg ttttccgaca agaaaatgga cacatcagcc tggcaactaa
    21121 ttctgcattg gaaatggcag aaggagagtt tatagcgctc ttagataatg atgacgaatt
    21181 acctccattt gccttatatg aagtggcaaa agtgctaaac gtacatccag aactggattt
    21241 gatctacagt gacgaagata aaatcgatgc agatgggaat cgctttgacc cgcacttcaa
    21301 agcagactgg tctcccgata cattgatggg aaataattat atctctcact taggcgtata
    21361 tcggacaagc attgttaagg aacttggcgg tttccgtaaa ggatatgaag gctctcaaga
    21421 ttatgatttg gttcttcgtg tgacagaaca aattcctgca gaacacattt accacattga
    21481 ccgtgtcttg tatcattggc ggacgatccc gggatcaact gcaagcaatg gagaagctaa
    21541 aagctacatc tatgattctg gagtgaaagc attgacagat gctctatcta gaagaaatat
    21601 caagggaagc gtacatcctg gcagaatttc tggtttttat gaaatcactt atgatgtttt
    21661 gcaagaagac ttggtcagtg tgatcattcc aactaagaat ggctatgaag atctaaaaac
    21721 ttgtgtggat tcgatcatcg aaaaaacgtc ttatccaaat tatgaaatca ttattgcaga
    21781 taatggaagt acagatccta aaatgcaaga attatttgca gaatacaagc atcagctgaa
    21841 agaccgattt atcgtcgaat taattgacat tccattcaat tattcacgta tcaacaatct
    21901 ggcagcagaa aaagcaaacg gcaaatactt tttattcttg aataacgata cagaagtgat
    21961 tgaacctgac tggatgacag cgatggtttc ttatgcacag tttgatcgaa tcggctgtgt
    22021 aggagcaaaa ctgttttatc cagatgatac aacgcagcat gcaggagtat tattaggaat
    22081 cggtggtgtt gcagggcatg cattgaataa ctatgatcga acacattgtg gttatttcgg
    22141 ccgattggtc attgacgtca attacttagc agtcactgca gcttgtatga tggtcaaggc
    22201 agctgacttc aatgctgtta acgggtttga tgaaacatta gaagtagcat tcaacgatgt
    22261 tgacttgtgc ttgaaagttt atgaactggg tcgttacaat gtctatgcac atcaagcaga
    22321 attgtaccat ttcgaatcta aatcaagagg ttatgaagat actccagaaa aacaaagacg
    22381 ttttgctggt gaaatcaaaa aaatgcaaga taaatggcca gcatatattg cgcatgatcc
    22441 attttacaat gataatttaa caaaagaagg aatcggcgac ttttcgttga gaccagatta
    22501 aactataata aaaagctgtt atgttgtgat tcgctcgcac ataacggctt tttatagttt
    22561 tttgtcaaaa aaaggcagaa ggtgccgact caaaaaaata agtcgaggtt gggacagaag
    22621 tgtttaactc cgagaaataa gaagaaattc acgaaaattg cttttcaaat ttttgtgaat
    22681 ttcggcttat ttaagaagaa gttgcttctg ctcccaccgt ttattcgttt ttaaagaatg
    22741 agacaaaagt gtttcttact tttgtcccac gctcttattc atgctcagag cgccaataga
    22801 tacaaaatca tagccaaagt ttcacgaacc ctagctgtca aagaatcgat attttatttt
    22861 aatataggct attttaaaat aaatggttta aggtattcct ctacaaaagc cactctatca
    22921 attatcatgg gtttaaaata agaagtgatg gagataacca gattgttctt agcgtcaata
    22981 taaagaacat ttccactatt accaattgct gaatatattt cgttctcaga atctaatacc
    23041 caccataaat agccatatct catattttgt aagtcttcta aagcactggt tctaggtttg
    23101 aacatctcgt ttatccacat gggtggtacg atttgttttc ctttatacat ccctttattt
    23161 aggcataata caccaatttt tgccagatca cttgccgata aacacaaacc atatcctgcc
    23221 gtcccgatat ttagtggatc acaaaaccag acattggttt taggagactt atccattgta
    23281 aactgcttat gttcttcagc tgtttcagca taataattga catggtttgg tatccctata
    23341 ggatcaaata gatacttatt ggcaaaatca acagttgtta gcttacttgt ctgatacaac
    23401 aagccagtta atatgtgtaa acagacagtt tgatagttga attcgccggt tatacctttt
    23461 cttcctccca ataaatctag tgatgttttt gtccaatctt cacttgaaca gacttttgtc
    23521 catggatcgc ctttacattt gtagggtgct ttcattgtta gcaagtgctt gattgtaata
    23581 gcgtaaatag ttttctcccc gcgtttcact ttatactctg gaaaataatc taatacttta
    23641 tcatcgatac ttttgattaa tccttgctct aaagcaatgc caatcagcaa agatacaata
    23701 cttttcgttg ctgacatgat atgacaagta tcgtcttttt tataaccatt ccattcatct
    23761 gtataaactt ctaccccatc cttatatgcg acaatttgac atatatttgg ttgtcgaaaa
    23821 ccaataaaat catgcaactc ttgtttattc ataagatatt acctcacttt cttctataag
    23881 cataatgtca ttaaaaatcg atttcaattt tttcgattct tcacgtaata caggtattct
    23941 tatggactag acagcgcttt gggaagatgg attctctgaa aaatataaag gttcacttta
    24001 tgagtggata aaaaaagcag caaatacttc gacttcttta atttaccaac attagtgaaa
    24061 atcgattcat ctggagattt gtaatttaga gtccttgtct tgattagagg tgtataaagc
    24121 tttttattag acatttttaa attccattac aatttgctta taaactaggt ttttaaaggt
    24181 gactatggta agataataga gatattattt tgtgattagg agtgtatata tgaataaaaa
    24241 tggagaatgg agtgctgcac gacggatatt cattatatta atggatgtta ttgtatacaa
    24301 tgcagcaata tatagtagtt tcttactgaa gtttcgtgga gagatcccat tacgaaattt
    24361 agaaactttc caacactcag caattattat ttcagtgatt tttattgcgt tgaacatttt
    24421 gttgggtgca tatgtttttt ataatcgaat gatcagtgac atcgtgtttg tcacagtcat
    24481 cggtcaggta ttgatgacac tagggatcat ggtagtgact tttgcgggcc gctggtttgc
    24541 ttttccacgt cttgttatct tactttcatt tgttctcggt acgcttttct taagtatcta
    24601 tcgtattctc gtatataaac tttatttacg cgtaagtcga gataaaaagg tcgtcattat
    24661 tgggttagaa aaggatgtcg caccagcaat ccaaaatttt ggttcaaaga aaaacaacaa
    24721 acataaagtc gaagcagtca ttcttgataa ctattatgca aacatcaaaa atatgattaa
    24781 agacatcgat attgtctatc ttgcttctca tatcgaagaa actgaaaaaa tcaaaatcta
    24841 tcaattagtt actaaaaatg aaaaaaaatt gtttttgaat acaacatttg aaaacttgac
    24901 attgatcaat ccaaatatta tgaatttcga agatgaaagt attattgaag catctggatt
    24961 tagaatccaa cctgaatatg aactattcaa acgtttgttt gatatcatcg tttcacttgt
    25021 tttattagtt attgcttcac catttatgtt attcacagca attttagtca aagtgacatc
    25081 accgggacca atcatctata aacaagtgcg gatcacaaaa aatcaaaagg aattttcaat
    25141 ctataaattc cgctcgatga ccgccacagc cgaagcaaaa tctggaccag ttttagctaa
    25201 gagcaatgat gcacgagtaa cgcctgtagg taaatttatt cgagctgtcc gctttgatga
    25261 attaccacaa atcttcaatg tgctgaaagg cgatatgtcg atcgtaggac cacgtcctga
    25321 acgtcctttc ttcgtcgatc agttcaatgc agaaaaccct tattactact tacggcataa
    25381 tgttcgtgct ggtatcactg ggtatgcaca agtctatgga aaatatgtct ctgattacaa
    25441 cagtaaatta cgttttgatt tactttatat caaaaaatat tcattgatgc ttgatttaaa
    25501 gatcttgctg caaaccatca agatcctatt tgataaaatg tcctctcaag gacttgaaga
    25561 agacgaaaat gtgatctcat tggcagaaat tgcttttccg aatgaaaaaa tctttcgcta
    25621 gttgggaggc gtaaaaatga acaatccgaa attcagcata atcgtaccag tatacaatgc
    25681 agaagcaact attggtcgaa cgattgatat tctaaaagaa ttggattatg aagagtacga
    25741 agtcgtttta gtaaatgatg gatcaacaga tcaaacacag gaaatcattc aagaactgat
    25801 tgaaaatgat gaacgttttg aattgattac tacgaccaat caagggcccg gattagcaag
    25861 aaacgaagga ataagacagt caaaaggaga atatctctta ttcttcgatg cagatgacac
    25921 gccagtaaaa acgatcttgt cagattatga tcatttgcta gaaaagagtc caaaagcgga
    25981 tcttgttgtt agttcgtttg tttttcgtac aatggacgag ggaagaatcg tttccgaaaa
    26041 agaaaactta gttacagaat gtcactatct aactcacgaa gagtttataa aagacatgta
    26101 tcagttaatg aatgaacagt tgatgtatgt tgtatggaac aaatgctatc gtagcgatat
    26161 tctgaaaaat aaccaaattc tatttaaaac ttataatagt tgtgaggatc gaatatttaa
    26221 cttacattat tatgaatact gtcatcaagt gatcatgaac ccaaaagtag aatatattta
    26281 tgaatttgaa ggtgggaagg ggatcactaa tcaataccga cctaacaagt tcgaaacatt
    26341 taaagaattc tattcattag caaatactgt aacagacgat attaataaag acggtatggc
    26401 ggcgcttctt ttaaaaggga caacttcagt tattttttct atttatagta cctctgcaag
    26461 aacttcaaaa gagaagaaag acgaagcaaa gcagatattg agtgatccag tgatccgaga
    26521 agccaagcaa attgctaaga cggattcttc agcaaaaaaa ataacaaagt tgctttacaa
    26581 tatgccagct ccactatttt tatctgctgt aaaagttgga agcttcgttg aagtgaaaat
    26641 gccagggtta atggctgctt tgaagcgagt atattagtta atggaggaaa atataatgaa
    26701 aagaattttg cattttcagg gacgaatggg tttaggtgga gcagagtctt tcatgatgaa
    26761 tttatatcgt aaaattgatc gtacaaacta tcaatttgat tttttgattt atgaagatta
    26821 tgcagatgtt caagactatc attcagagat tgaaagatta ggaggccgta tttttgttgt
    26881 acctaatcct aagaaaaata tttttaaata tttaatagaa gtgaataagt tactaaaaaa
    26941 agaatctttt tctatagttc acaatcaggt ttattttggc gggggaatta atttatggtt
    27001 agccaaaaag aatggaatca gacagagaat tgctcatagc catgcaactg aagatggaaa
    27061 atctcaaaat attgtaatga atgtattgcg taaattttta accaaattgt tgttacaaaa
    27121 tgcaacagac taccttgccg ttagtcaaga agctggtgaa agtctgtttc aaaaccatcc
    27181 ctttgagata gttcataatg gtattgattt agagctttat agtaaaaatt cagaagctaa
    27241 ggttaataag agaaaagaat tggatatctc aatgagtact tttgttgtgg gaaacattgg
    27301 acgattagag aaacaaaaaa atcaatctta tttgttagag atttttttag aaatactaaa
    27361 aatgaattct gattcttact tgattttagt aggtgaaggg tcacttagaa ataaactgaa
    27421 agccaaagca gtagaattgg atatagagaa aagagtaaaa tttttaggag aaagaaacga
    27481 tattcccgag ttattatctg tgatggatgt ttttgtaatg ccttctcttt atgaaggtct
    27541 gccaatggtt ggattagaag cacaagcagc aaaattaaaa ttagtcttag cagatactat
    27601 ttccaaagat acaaagttaa caaataatgt ggattttgta tcattagatg aaacaccaca
    27661 agaatgggca aaaaaagtaa taaaagagcc gtatgctaat aaaataacac aagaactaaa
    27721 gagttatgat gtttcgtaca ctactgaaca aatggagagt atatatagta gagatcatta
    27781 aaaatgttca agatatttta ggagtgatta tttttgaaaa tatgttattt gatattggca
    27841 tacaatgata ttggcaactt aaagagattg gtcaatcgat tgaacgaaaa tgcagttttt
    27901 attattcata tagataaaaa aactgatata gaaccattta ttgaagcatt taaatcatat
    27961 gataatgttg aatttttatg taataaaaac cgtgttaaaa tttattgggg tggtttttcc
    28021 attgtacaag cagaaatcaa tttagtaaag agagcattac aaaatgagaa atatttaaaa
    28081 tatgtattat tatctggtgc tgattatcca atcaaagata atgagtatat ctataattat
    28141 tttaaaaaga atagttcagt gg
//
LOCUS       DACDUU010000032        28122 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.032, whole genome shotgun sequence.
ACCESSION   DACDUU010000032 DACDUU010000000
VERSION     DACDUU010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28122
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.032"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..214)
                     /locus_tag="IWL30_001431"
     CDS             complement(<1..214)
                     /locus_tag="IWL30_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706206.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYK"
     gene            482..2491
                     /gene="metG"
                     /locus_tag="IWL30_001432"
     CDS             482..2491
                     /gene="metG"
                     /locus_tag="IWL30_001432"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAP9706207.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(2970..3557)
                     /locus_tag="IWL30_001433"
     CDS             complement(2970..3557)
                     /locus_tag="IWL30_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706208.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            3732..5054
                     /locus_tag="IWL30_001434"
     CDS             3732..5054
                     /locus_tag="IWL30_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9706209.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            5209..5985
                     /locus_tag="IWL30_001435"
     CDS             5209..5985
                     /locus_tag="IWL30_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP9706210.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            5978..6547
                     /gene="rnmV"
                     /locus_tag="IWL30_001436"
     CDS             5978..6547
                     /gene="rnmV"
                     /locus_tag="IWL30_001436"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAP9706211.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            6540..7424
                     /gene="rsmA"
                     /locus_tag="IWL30_001437"
     CDS             6540..7424
                     /gene="rsmA"
                     /locus_tag="IWL30_001437"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAP9706212.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            7875..8504
                     /locus_tag="IWL30_001438"
     CDS             7875..8504
                     /locus_tag="IWL30_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706213.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(8628..9737)
                     /gene="ugpC"
                     /locus_tag="IWL30_001439"
     CDS             complement(8628..9737)
                     /gene="ugpC"
                     /locus_tag="IWL30_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAP9706214.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            9946..10368
                     /gene="mgsA"
                     /locus_tag="IWL30_001440"
     CDS             9946..10368
                     /gene="mgsA"
                     /locus_tag="IWL30_001440"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAP9706215.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            10691..11227
                     /locus_tag="IWL30_001441"
     CDS             10691..11227
                     /locus_tag="IWL30_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP9706216.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(11267..13030)
                     /locus_tag="IWL30_001442"
     CDS             complement(11267..13030)
                     /locus_tag="IWL30_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9706217.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(13033..14748)
                     /locus_tag="IWL30_001443"
     CDS             complement(13033..14748)
                     /locus_tag="IWL30_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9706218.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(15527..15751)
                     /locus_tag="IWL30_001444"
     CDS             complement(15527..15751)
                     /locus_tag="IWL30_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706219.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            15951..18719
                     /locus_tag="IWL30_001445"
     CDS             15951..18719
                     /locus_tag="IWL30_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9706220.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            18800..19219
                     /locus_tag="IWL30_001446"
     CDS             18800..19219
                     /locus_tag="IWL30_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAP9706221.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            19231..19701
                     /locus_tag="IWL30_001447"
     CDS             19231..19701
                     /locus_tag="IWL30_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9706222.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            19718..20497
                     /locus_tag="IWL30_001448"
     CDS             19718..20497
                     /locus_tag="IWL30_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9706223.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            20484..21305
                     /locus_tag="IWL30_001449"
     CDS             20484..21305
                     /locus_tag="IWL30_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9706224.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            21322..22371
                     /locus_tag="IWL30_001450"
     CDS             21322..22371
                     /locus_tag="IWL30_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9706225.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            22384..23388
                     /locus_tag="IWL30_001451"
     CDS             22384..23388
                     /locus_tag="IWL30_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9706226.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            24021..25040
                     /locus_tag="IWL30_001452"
     CDS             24021..25040
                     /locus_tag="IWL30_001452"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP9706227.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            25083..25964
                     /gene="galU"
                     /locus_tag="IWL30_001453"
     CDS             25083..25964
                     /gene="galU"
                     /locus_tag="IWL30_001453"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP9706228.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            26264..26641
                     /locus_tag="IWL30_001454"
     CDS             26264..26641
                     /locus_tag="IWL30_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706229.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            27247..27861
                     /locus_tag="IWL30_001455"
     CDS             27247..27861
                     /locus_tag="IWL30_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9706230.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT         9195 a         4857 c         5780 g         8290 t
ORIGIN      
        1 ctttataccg ctcttcacga atggcaaaga agccacaaat aacgatatat acgtcaatga
       61 taaaacgcaa aatccaagct gctgtcataa aactgttcaa atgcccatgg aggttaaaat
      121 aaccatacat acgaatataa aaatagctga atattaaatg tgctaatgaa agaaaagcaa
      181 tactaagtgt aaaaaaaaga tgtttcttct gcatgattgt tccagtccct tactaagata
      241 gatagtcttt attataccac gtgtgagggg agaaataaaa agccgttttt tagtagatat
      301 gaaacaaaac aaaaacccgc atttattcaa cctattcatc cttttaaagg atagattgaa
      361 cgtagataga agtcgtttat ccagtctttt tcattttgaa aaaaagctaa cagcatgctt
      421 tttttatggt atactagcaa ggaaacgaat tcgcatcatg cgataaagga ggaagagccc
      481 cgtggcagaa aaggaaactt tttatatcac tacgccaatc tattatccaa gcggcaaact
      541 tcatatcggc aactcgtata ctacgattgc atgtgacgca attgctcgtt ataaacggtt
      601 gatgggcttt gatgtgtttt atctcactgg agtggatgaa catggtcaaa aaatcgaaac
      661 aaaagcagaa gaactaggtg taaaaccgca agaatatgta gataaaatgg cggctgatgt
      721 caagaaatta tggaaaacgc ttgatatcag ttatgataaa ttcattcgca cgacagatga
      781 ttaccataaa gcagctgttc aaaaaatatt tgatcgttta ttagaacagg gagatattta
      841 cttaggtgaa tatgaaggct ggtactctgt ttctgacgaa gaattcttta cagaaacaca
      901 acttgcagaa gtgtacaaag atgacgaagg taacgtgatc ggtggtaaag cgccaagtgg
      961 acacgaagta gaattggtaa aagaagaatc atacttcttc cgcatgagca aatacgcaga
     1021 ccgtctattg gaatactatg aagaacatcc cgaattcatc cagccagaat ctcgtaaaaa
     1081 cgaaatgatc aacaacttta tcaaaccagg attagaggat cttgcagttt cacgaacaac
     1141 ttttacttgg ggagtaccag tcaagagtga tccgaaacat gtggtgtatg tatggatcga
     1201 tgcattatcc aattatatta ctgcactagg atatggctcc gaagatgaaa gtctattcga
     1261 aaaatactgg cctgcagatg ttcagatggt cggtaaagaa atcgttcgct ttcatacgat
     1321 ctattggcca atcatgctga tggcccttga tttgcctcta cctaaaaaag tatttgggca
     1381 tggctggcta ttgatgaaag acggaaaaat gtctaaatca aaaggaaatg tcgtttatcc
     1441 tgaaatgctt gtggaacgtt atggtttaga tgcactccgt tattatttgc ttcgtgctgt
     1501 tccgtttggg tcagatggtg tctttacgcc agaagatttt gtttcccgtg tcaattatga
     1561 tctggcaaat gatctaggga acttattgaa tcgaacgatt gctatgatca acaaatattg
     1621 tgatggactc gttcctgact atgcctcact tgtcactcca tttgatagtg aactgtctac
     1681 gacggctgct aacgttgtcg gacgttacca tgatgcaatg gaaaaaatgg aattcaatac
     1741 agcattggct gaaatctggg tgctgatttc tcgtgcaaac aaatacattg atgaaacaga
     1801 accatgggta ttagcaaaag acgaagaaaa gaaagctgaa ttgaatagtg tcatgatcca
     1861 tttagctgaa tctttgcgta tcgcagcgat attacttcag ccaatcatga cagaaacgcc
     1921 agagaagatc ttcaatcaac taggattgga ctctgaaaca atgaatcttg aaggattaca
     1981 ctttggcgaa tttccttcag gaacaaaagt agttgcaaaa ggtacaccaa tcttcccacg
     2041 tttagatatg gaagaagaag ttgcttttat tcaagaaaaa atgtctgaag gtactcaaac
     2101 aaacgaagat acagtcaaat gggatccaga agaaacagaa cttgtttcaa cgaaagaaaa
     2161 acaaatcaaa tttgatgtat ttgaaaaagt agaactaaaa gtagcagaag tcatcaactg
     2221 tcaaaaagta gaaggggcag ataagttgct gcaattccga ctagatgctg gagacagcca
     2281 agaccgtcaa atcttgtcag gaatcgctga attttatcct gatccaagcg aattgatcgg
     2341 gaaaaaagtc gtcattgtag caaatctgaa accacgaaag atgcgtggac aaatcagtca
     2401 aggaatgatc ctttctgcag aagcaccaga tggctcttta caagtaatcg aagcaccaaa
     2461 atccatgcca aatggctcag aaatcgcata gaaattcaaa gggggtatga cactagttgt
     2521 gtcatacccc ctttttccga aatttgagaa gcaattttcg taaattcctt cttattactt
     2581 gaagccaatc atataataca gaaacagaga actatccaaa ataaatccta gaaaaagaag
     2641 tacgagcttc gctgattttt gttaatttag aataatacta ataggaatcg taaaaacaga
     2701 aaaaaagcat aatgataaag ggtcgggcag attttaattg attcatctgg tactcttttt
     2761 tattgtcctt acattcataa agtttgaatg caatgacttt attacatcta tatttatatc
     2821 taacagtagc gttgatgtgt taattataag ttctatcact ttatgttcaa ttagtttgtg
     2881 ctcttatatg tgtgattgaa aatatagata aaaaaacaat catcggcttt gacttcgatg
     2941 attgttttct ttattttgta aaaatcctat cacttgattt tatggtaacc ttttattttt
     3001 gtcttcaaac ggcttggtaa gagagcagta agcagtgcaa atgggatcga aaagacatac
     3061 cagaaaatcg tacgtccgat accataatgt tgatagagct ttgtcaacat cacgtgtaac
     3121 aagatcaacc ctaaaagcaa ggagccagct ataaaccaat gattatccca atgcatgact
     3181 tcactaagtg ccatcactcc aaaaggatag aaataaaaat agatcccgac taaggtcatc
     3241 cgccagttct ttgtaaatgt tccgatattc aataaaaaga aaatcgctga aagcaaaata
     3301 ccgataggcg gaaaaagccc aaccaaaaag gaatacaaca tcccccacaa gatcatcaca
     3361 ggacctttga tcaatgcaac gattgcgatg aacaaagtca gcaatattag tttttgtgat
     3421 aacgttgcaa cagtcattaa tccaaataaa attaatgtga accaatgcca atgggactgc
     3481 tgttgttctt tacgggaaaa atcgacccgg ttggataaat aatctgctaa tttcggataa
     3541 gaacgcttaa aatccataaa ctgttcctcc ttcgtggaaa aggtaggttt agtatacgaa
     3601 aaataaaaaa gaaatacatc cgtcttaaga gggagttttt cactgaaagt ggagaaaaaa
     3661 gaatggtaaa ttaaaatatt ctcagttata ctgaaaatta taccaaattt caaagaagaa
     3721 agaggatggg aatgaaaggc aaactaaaac gagaaatcaa tctgtttggc gcattagcta
     3781 ccgttatggg aaccgtgatc ggtgcaggcg tcttcttcaa aactgctgct gttactgcta
     3841 gtactcagtc tacaagtttg acattgctag cttggctttt gggtggtttc ttaacgatct
     3901 gtgctggatt gacagtggct gaattagcta cagcaatccc ggaaactggt ggagctgtaa
     3961 aatatattga agctgcgtat ggaaaattgc ccagcttttt attaggatgg gcgcaaagct
     4021 tgatttactt tcctgctaat attgctgcat tatcgattat ctttgcgaca caattgacga
     4081 acttattaca attatctacc gattatcttt tgttgattgc agtgatcacc gctgtttctg
     4141 tgacagggtt gaatttgtta gggacaaaag tcggtgcttc tgtacaatca gtgacgttga
     4201 ttgttaaatt aataccaatt gctgtgattg tcatatgggg attattgacc ccaggacaag
     4261 gaactgtcca gttatttcca atcgaagctg gaaaagatgt tacttttgtc gaaggattga
     4321 gcagtgcgtt gttagcaacg ttgtttgctt atgacggctg gttaggtgtc ggtgcaatgg
     4381 caggggaaat gaaacggccg gaaaaagatt tgcctaaagc aattattctt ggtttgagtt
     4441 ttgtaacagt cgtttatcta ttgatcaatt ttgttttttt gaaaactttg ccgattgatc
     4501 atcttgccgg taatttgaat gcagcttctg aagcatcaga cgtcattttt ggaggaatcg
     4561 gtggaaagct ggtcactatt gggatcttga tctcggtata cggtgcattg aacggttata
     4621 cactaactgg gatccgcgtg ccttatgcga tggctttaga agacgagcta cctttcagta
     4681 aacagctgac aaagctttca aagaagttta cggttcctta tgttcctgcg gttttccagt
     4741 tagcagtagc ttgtattatg atgagccttg gttctttcga ctttttaaca gacatgttga
     4801 tttttgtgat gtggctattc agtttactga tttttatcgg tgtctttatt ttaagaaaaa
     4861 aagcgccgga attgccacgt ccatataaag taccacttta tccaatcgtt ccgattattg
     4921 cgattctcgg tgctattttc attttaggta tgacgatgat gacgcaaacg aaattagcat
     4981 tgatcggtat aggtgttacc ttgataggta tcccagtcta ttatcaaaaa aagaaaaaac
     5041 gctcagaaga gtaaaatatt tcaacaacag acagaaaaag tacgaactag gtcctctaga
     5101 tcttcgtgct ttttctattt caataaaaca agttcttcta ttttgatttt tgctacttct
     5161 gcaagtagtg cttatgtgct aaagtaagaa tgaaaaggag agataaacat gatatttgat
     5221 tctcataccc atttaaatgc tgaacagttc aatgaagata ttccagaaac gattcagcga
     5281 gcacaagaaa tgggggtaac gaaaatggcg gtcgtgggct tcgatacgcc aacgattgaa
     5341 aagtctttgc aattaagcca tgactatcca aatatataca gtattatcgg ctggcatccg
     5401 acagaggctg gcagttatac gaaagaaatc gaaaataaat tacaagaaca attgacttta
     5461 ccaaaggttg tagcattagg ggaaattggc ttggattatc attggatgga agacccgaaa
     5521 gaagtccaag ataaagtatt tcgcagacag attgctatcg caaaagaaat gaacctacca
     5581 atcagtattc atactagaga agcacttgaa gacacgtatc aaatcctaaa agaagaagat
     5641 gttcgagata tcggtggaat catgcatagt ttcagtggtg attatgaatg ggcaaaacga
     5701 tttcttgact tagggatgca tatttcattt agcggtgtcg tcactttcaa aaaagcatta
     5761 gacgtccaag aggctgcagc aaatgtacca atcgaacgat tacttgtgga aacagatgcc
     5821 ccttatctgg caccagttcc ttttagagga aagcgaaatg aaccaggcta tactcgttat
     5881 acagtagaaa agattgctga acttcggaat cttccgacag aagaagtggc tgcccaaaca
     5941 tggacaaatg cgcaccgatt atttggtcta agcgaacatg gataagatga agatccaaga
     6001 gattatagtc gttgaaggaa aagacgatac aagaaggctg cgggaagtag ttgatgcaga
     6061 tacgatagaa acaatagggt ctgcaatcaa tgaagagatc ctgatgcaaa ttgaacatgc
     6121 ccaagagact cgtggagtga tcgtttttac cgatcctgat ttttcaggag aaaaaataag
     6181 aaaaatcatt atggaagctg ttcctcaggc aaaacatgct tttttgccgc gcacacaagc
     6241 taaaccaaag aaaaagggaa gccttggtgt cgagcatgca agtgatgctg ctattttaga
     6301 agcattgaaa aaggtcgtga cgccagacat ttcccaaacg gctgtttctg aaatcactag
     6361 acaagacctt ttagactatg gtttaatagc tggggcacaa gcaaaagaaa aacgagaaaa
     6421 actaggtgac gagttgcgca tcggttacac gaacggcaaa caattggaaa aacgtttgaa
     6481 gatgtttcgt attacgccga aagaactcgc ccaagcaatg aaaatagtgg aggaacaaca
     6541 tgagtgaata tcgtgaaatt gcgacaccct ctagaacaaa agagatttta ttgaaacatg
     6601 gtttttcatt taaaaaaagt ttaggacaaa attttctgac tgaaccaaat attttaagga
     6661 aaatcgtaga aactgcgcaa atagatgaac atacgaacgt gatcgaagtt ggaccaggaa
     6721 tcggtgcatt gacggaacaa ttagccaagc atgcaaaaca agtcgtagca tttgagatcg
     6781 atgatcgttt gatccctgta ttggctgata cgatgcaacc ctatgacaat gtgactattg
     6841 tacatcaaga tattttaaag accgatttgt caacagctgt tagagaaaca tttcatgagg
     6901 agcttccgtt aaaagtcgta gcgaatttac cttactatat tacgacaccg atcatgatgc
     6961 attttcttga atcagatttg gttgtggatg aactagtcgt catgatgcaa aaagaagtag
     7021 ctgatcgtat ctctgctgaa ccaggaacga aagcttatgg ttcattatcg attgctgttc
     7081 aatattatat ggaagccagt ttagcattca tcgtaccaaa aacagtattt gtccctcagc
     7141 caaatgtaga ttcagcgatt ttgaaactga caagaagaga tataccggca gtcgaagtga
     7201 cagacgaaaa agcatttttc cgattgacaa aagcggcatt tcagcaaaga agaaaaacgc
     7261 tttggaataa cttgcagcat tcgtatggca aagatgacca gacaaaagca tggttagcaa
     7321 aaagcttgga aacagctgga atcgatccaa aacgtcgtgg ggagactttg tcattacaag
     7381 aattcgccgc attaagcaat gcgatgtcag aaaatatgca gtaaaaaaag aggttgtgac
     7441 agaagttgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca aaaatagttc
     7501 ctcatgtttt tcgtttgttt gggcttatta ccgcagatgc aagcttttga acaccgttta
     7561 agaaagaggt tgtgggcctg ccgtttaact ctaagtcaca aggaacaatt tcaaaaaaca
     7621 gcttctcatg tttttgagtt agttggtctt attacttgat gcaagctttt gaccactatt
     7681 atgccgaaca aggagatgtg acacaaattt tgtcacatct cctatttttg tttcttgatc
     7741 aaaaaattct tatacttgat actcacactc attttgtatg gatttagaaa agaatatgtg
     7801 tcttgacagt tattagtaag tgagttatac taagtcgaca tttatgtgtc aagacatatt
     7861 aggagagaga aaaaatgaga aaaaattcag ttcgaaaact aacgatttct gctttattga
     7921 ttgggatggg ggtcattatc ccaatggtca tgcctaaaat catgattcca ccagcttcct
     7981 ttactttggc gtctcatgtg ccattattta ttgcgatgtt ttttacacca ggagttgcag
     8041 tggctgttgc gctaggtacg acatttggtt tctttttaac aacgccggtt atcatcgcat
     8101 tgcgagcatt gtcacatctt gtgtttgcgt tgattggggc ttggtacttg caaaagcatc
     8161 cgagtatcgt tttaaaaaat ggacagttta cttttgctaa ttggcgtttc caagggttca
     8221 attttgtgat cggtgtgatt cattctttag cagaaatgct agtagtcagc atcttttact
     8281 ttattggtaa tatgcctgaa acatattatt ctcaaggata tttttataca gtattcatct
     8341 tgatggggat tggtgggctt atccacagtt tagttgatta taatattgct tattttatag
     8401 caggaacact cagcaaatat tttgatatcc ccgtatttac ggcagcaaaa aaagaaaatc
     8461 ggaaagagtc gattcaaggg tacgcgaaga aaacgacata ttagacatga tctctcaaaa
     8521 aaaaaactct catctagtaa tggataataa atcctctaag atgagagttt tttgtcctaa
     8581 aaagagctgc accaacatct tctggcacag ctctaacagg gggcaattta tggaagtttg
     8641 atgacttgtt ctgtttcttt atcaaagaag tggcctttgc tgatgttgaa ggcaaggtcg
     8701 actatttcgc ctggtttatg gaaatctctt gcatctactt ttgagatgaa ctcagtagaa
     8761 ccagtttttg tgtaaagcat cgtttcagct ccaagtaatt cggaaacgac aacttctgat
     8821 ttcactacgg cttcaggtga agcttcaagg gctacttgtt cactgtggat gtcttctgga
     8881 cgaataccga agataacttc ttttccttca taaccttttt caactaatac tttgtttttt
     8941 ccttcaggaa tacgaagctt aagcccatgt ccatctgtga taacaccatt tttcaatgtc
     9001 acattaaaga agttcatggc aggagatcca ataaagccgg caacgaacat attgacgggt
     9061 gtatcataaa cttctttcgg tgtaccgatt tgttggataa atccgtcttt catgataacg
     9121 attcggtcag ccatagtcat tgcttctgtc tgatcgtgag taacatagat ggtcgttgtt
     9181 tcaagacgct ggtgaagttt ggcaatctca gcacgcatgg ctacacgtaa tttagcatct
     9241 aaatttgata atggctcatc cattaagaag acttttgcat cccgaacgat tgcccgtccc
     9301 aatgccacac gttggcgttg tccaccagat aaagcagctg gttttcgttt caagtattct
     9361 gtcagaccta agatctctgc tgcattgtcc acacgttttt tgatgtctgc tttatcatat
     9421 ttacgtaatt tcaagccaaa agccatatta tcaaaaacag tcatatgagg atacaatgcg
     9481 tagttttgga aaaccatggc aatatcacgg tctttaggtg ccacgtcatt catgactttt
     9541 ccgccgatat ttagttctcc ttcagagata tcttccagtc ccgcgatcat tcgcaaagta
     9601 gttgactttc cacaaccgga aggaccaaca aaaacgataa attcacggtc tttgattgat
     9661 agattaaaat cagttacaga ataattttct gcattgtcat attttttata tacgtttttc
     9721 agagccattt ctaccataat acgttccaca ctttcttttt tagttttatt aactatttaa
     9781 agtataaatg aaagcgttaa ctaacttcaa tgtcatgttg aacaagaatt ttttcttttt
     9841 tttgtcaatg tgctagtttt aagctttaat aaatatccat aagaaataaa aactgataca
     9901 ctggagtaaa taagtatgtt aacaatatca aaaggagtgt ctcttatgaa aatcgctttg
     9961 atcgctcatg accgaaaaaa acctatgatg gtgaaattag caacagcgta taaaacaata
    10021 ttggaaaaac atgaactttt tgctaccggg acgactgggc agaggatcac ggaagcaacc
    10081 ggcttgccga tccatcggtt taaatccgga ccgttaggcg gggatcagca aattggagcc
    10141 atgatttcgg aggatcagtt agatttggtg atttttcttc gagacccgct ggctgcacag
    10201 ccccatgaac cagatgtaac agcacttatc cgtttatctg atgtctatga gattccgtta
    10261 gctacgaata tcggaacagc tgaggtgcta ctaagaggac tggaagccgg cttcctagat
    10321 tttcgaagaa tcgtccatga aatagacaag catccacttt cattttgatg gataagcgta
    10381 tggaaaaata acgtttaatg ttcgcctata tgtagctata aaagctaaac aaactgataa
    10441 tatgcgatat aatacgaacg tattggataa tataaacaat aatattagtt tttcgtattt
    10501 gcagtaaatg tgaaaatatg ctatagtttc tgtgggaaaa gatcccaaaa ataatacaga
    10561 gtagaaaata aagcgttaag tgctaagagg atgggatgtt gcctcttgga cgaaggacgt
    10621 aatgttcgcg gttttatttt cgcattcgct gcatgatacg atgtagcagc tcttaaagga
    10681 gatgctaaaa atgaagaaac accggttaga cgcacgtatg attgctatta tgggcttatt
    10741 gattgcattg atggtcactt tgtcccgact ggtagcaatc gagaccccat ttattaaaat
    10801 cagtgtcaca tttattccac aagtgatcat gggcattctt tttggtcctt tttggtcagg
    10861 gataggcgca gttttagctg atttagtagg aatggccctc ttctcgaaat ctgcattctt
    10921 tattggattt acattgaatg catttattga aggagcaatc tacggattct ttttctatcg
    10981 aaaagagatc acttggaaaa atgccattct ggctacgctt agtgtcacgc tgatcatcaa
    11041 cttatttttg acaccattat ggcttgcgtt gatgtaccat gtgccattgt tcagctgggt
    11101 cgtttgggcg ccccgcctgt taaaaacagt aatatggtta ccgattcaat caattgccat
    11161 ttattacgta ggacgtagta ttccttacaa aaaaatttta agaagtttag cgatccacgc
    11221 taaataaaaa acaagcagtc ttcccatccc agaagactgc ttgtttttat tgtgctgtga
    11281 attgtgcttg atagagccgt cggtaataac catctttttg aagcagctca tcatgggtgc
    11341 ccatttcgac aatagctccc tgatccatca ctaatatctg gtccgcattt ttgattgtgg
    11401 acaggcgatg ggcaatcaca aaactggttt ttccttccat cattgctaag aaggcttgtt
    11461 ggattttttt ctctgtcaat gtatcgactg agctggtagc ttcatctaag atcaacatag
    11521 ggggattact gatcatcgtt cgagcaatcg tcaacagttg gcgttgtcca tctgatatct
    11581 ttaacccttg accgccaatc ttagtatcta atttttccgg caaacgttct acaaattcat
    11641 acatataaga ggcttttagg gcatcattga tttctgcgtc acttgcttcc ggattcccaa
    11701 aacgaagatt ttctcgcaat gtactgtcaa atagccaagt atcttgtaac accattccaa
    11761 aacttttacg taatgtatca cgttggatag agcggatgtc atggtcatcg actgtaattg
    11821 ttccttttgt tgtgtcataa aaacgcatca gcaaattaac aagtgtggat tttccggccc
    11881 ctgtttttcc tactatagca atggtttgac ctggataagc agtaaaattg aaatcttcga
    11941 tcaaacgctg cttggggtca taagaaaaag aaacatgatc aaaagagact tttcctttga
    12001 tatttttggg ttccaaggca taggcatctg gcaaatcagg cttttcttct ggctgatcga
    12061 tcaaatcaaa tgctcgttct agaccagaaa aagcggtttg cagttgtgtc gtgattccag
    12121 aaagttcgat aaatggttta gaaaactgac ttgagtagat cgtaaagctg gaaatcacac
    12181 caacagtgat tgacgaacca ctcttcaatg ccaataggcc gccaactaaa ccaatcgata
    12241 aataagcaag gtgatctaca aaacgagaag acgggtttgt caaggaagaa gaaaattgtg
    12301 cccgttgtcc tttttcatat agatcagcat ttagcagttc aaattgctgt tgactagctt
    12361 cttcttgctg aaaagctttc acgattttct ggttaccgat tcgttcggtc acaaagcccg
    12421 agatatcccc aataatcctt tgctgtctat cgaaatttgt ttgtgaagca cgagcaacga
    12481 tccaactcac aaggaaaata attggtgtag aacaaagaac gaccaatgtc agaagtggac
    12541 ttagacgcat cataaaaata aacgaaagaa tgatgattgc cactccagaa aataattgat
    12601 tgaaggccgc agaaattgca atggaaatat tgtccatatc atttgtaaat cgactgacga
    12661 tatttccgtg ggagttttga tcataataac gaagtggcaa tcgattcaga tgagcaaatg
    12721 catcttttct taattttgca acggataagt aagctaaccg attgcttaac acttggatga
    12781 gccattgact gactacgttg attaaaacga taccaccgaa tagaataagt aaatggaaca
    12841 gtcgattgaa gcggacctgt ccttctccaa tcatttgatc gatactttga ccaatttgta
    12901 acgtcatcac aactgttgca atcccattga tgattcctag tatcacagct agccaaatgt
    12961 cttttgcata ggcagtaagg taaggaatga atcggcggag cgtctgcaag gataattttt
    13021 ttactttcat aactattctt cctcctgtga tgcgacgatt tcctgatatt ctgctgattg
    13081 agcaagtaat tcttcatggg taccctgagc agcaatctta ccttgatcca tcactaagat
    13141 atgatgtgct tcttgaatag agcggacacg ttgggagata ataatcactg tactgccaag
    13201 ataagtctgt agcgccttac gcaaatccaa gtctgtttga taatccaacg cacttaaaga
    13261 gtcatctaaa atcagtaatg ctggcttggc aatcaaagct cttgcaatcg tcagtcgctg
    13321 tttttgtccg ccagagaaat ttttcccatt ttccatgact tttgtatcta aaccatttgg
    13381 aagctgacgg acaaattctt ctgcctgagc gattttcaga gcttcccaac attctttttc
    13441 tgtggcgtct ttcttacccc attgaaggtt ttcacgaata gtcccggtaa atagtacaga
    13501 agtttgggga acaagagcaa tttgctgacg gagatgatcc agctgccaag aacgaacatc
    13561 cattccgtcc attataactg taccggaact gacatcatag aaacgcggaa tcaattgtgt
    13621 caaagtcgtt ttcccgcttc cggttggacc aatgattcca aggactgtat ttcgcttcag
    13681 ctgaaatgta atattttgga gagaaagacc agaatcagga gtataacgga agtcaacatg
    13741 gtcaaaagcg ataattgctt cagaagaaaa atcgggagta gtactgacct cagcttcaag
    13801 aatcgaattt tctgtgtcta gcacctcttt cacccgattt cctgaagctt cggcacgtgt
    13861 gaagatcacg acgaggtttg aaacaacgat cagtgccaaa agcatttgat tcatataatt
    13921 gatcaaagca agaacttgtc cttgttgcaa gctaccaata tttactttga tcccgccaac
    13981 tgtcagcaaa cagatgatcc cgacattcat gatcaaggtt gtagcaggtg ataatagggc
    14041 agagatattg gaaacacgta aataattgtt cgctagatca tttgtgcttt tatcgaactt
    14101 ctctgtttct ttttttgtct gcgcaaatgc gcggatgaca cgaacaccgc taagattctg
    14161 actaacgtgt tcattgagct gatctagttt ttcttgcact ttttggtaga gaggaacggt
    14221 ttttttgatg ataaaaaata aaatgattga gaaaataggc aataatgcta agaaaaacag
    14281 acctacttcc cagtcgatca caaaagccat gaccactgac ccgatactta aaaacggtgc
    14341 tctgatcaat aaacgaatga ccattgctaa agctagctga agctgattga tgtcgtttgt
    14401 cattcgggtg atcaaggtat ctgttccaaa atgattcaac tcgttgtgag agaaagtatt
    14461 gatttttttg attagctgat tacgtaactc tgtaccaaat ccttgtgaag ctactgaagc
    14521 atagtattgg caaatgatag cacaaatcag accaatcaca gacatagcga ccatccataa
    14581 gcccatttca atgatttttc ctcggtcatt catttttagt ccattatcga tcaaggaagc
    14641 catcattaaa ggcaggacaa gttcgaagac agcttcaaga aatttgaaga acgggcccaa
    14701 aatgatttgc ttacgatagt cttttgcata acgtaataac tgaatcatat gattcctcct
    14761 attatttatt tgtccacaat tgaaaaaaca agtactacca cattgtagct taggaaattt
    14821 agaatgaaaa gagaacaagc cttttcataa atgaaagttt tattatgaag aagctttaga
    14881 aattgaatag actatttttt ttaaaaaaaa gaaaatgaga aaacatgcca gtctcttcat
    14941 tcataggaac gtctatttta cttaattttt ctataaatga attctgcata aaaaatatat
    15001 caagtaaatg tttatagatg aaactctatt tacttctttt ttaatttgtt cttgcaatct
    15061 tttttgctac gtaatttttg taaaaaagtg ggttgagaga aatctaattg attttcaaag
    15121 aaggagtatg aaaatgagaa aaatatttat tcgtttattg tgggtactat cttagaggaa
    15181 taaataatag tggagaaaac tatggtttgg cgcagattct atcaggtttg atggaacgaa
    15241 gacaacctcc cgacatcaag tcaaaagcta cttgagattt tgatctgaaa ataaaaaaag
    15301 aaaacaaggt aggaacatca atgtttctac cttgttttcg taatcaatgg gaaaaaacta
    15361 agagtggtgt acgtaatcaa taaaaaggat ggtgacaaca gtacctcttt actctctgaa
    15421 agcatcataa ctcgtttttc ctcattatgc caataaattg ttgttatacc acatataagg
    15481 atttgtaata gctaaaaaca gacaggaaag ttgaaatctg tgaatcttat tttgtatgga
    15541 aggatttata gaacgcaaaa atcaagataa agatgaataa aaatcctgtt tcggctatat
    15601 attgcgtatc tacgccaata aaacgagttt gttctgtcat agccatataa agcaaaggca
    15661 aaaatacaac tacagcgaga taagatccga tgatttctcg aaaagttttc caggaagtat
    15721 gtttcctgtt tttatattta tccgatttca ttgaatagtc atagcctcct tattctttca
    15781 tatgttgaaa ttccagtata aaaactggaa ggaactgtac gttttttaga ataaaaatta
    15841 taagggataa gtaggctgct gtctataata agatatattt ttcaattctt tattaaagat
    15901 tttattgaca aaatgaatcc gttttctatg atggaggagg ggtgaataag ttgttgaaga
    15961 aggaaatttt atgttactta gagaaccaga cagccttttt cgatttagaa catatgaacg
    16021 aggtctttac agcacaaagg ttagctgatc attttggggt aaaaagaaac acagtcagtc
    16081 attatttgaa ccagttaaat gatgagcaac tactagtcaa aatcaattct cgaccagtga
    16141 tttatttcca caaagaagct tttgaaaaac aattttttaa gttacgcacg aatttttatt
    16201 tttctatcga tgatctcaaa gaggaacagc cttttttcgc gcaaccaaaa gacttattct
    16261 cttttatgat cggatacgac gccagtttga aagaaagtat cgaacaaatc aaaacagctc
    16321 tttactatcc agatggcgga ttaccacttt tgattacagg cgaaagtggc acaggtaaaa
    16381 gttatttagt taatttggtc tatcagtatt gtctgcttca tgatctgcta gaagattctg
    16441 caccattcat cacttttaat tgtgcacaat atgcagataa tcctgaactt ttgactagca
    16501 atttgtttgg acatgttaag ggagcgtaca ctggtgcaga agaaaataaa aaaggggctt
    16561 ttgaagcagc agatggtggg atcttgtttt tagacgaggt acatcgctta acaccagaag
    16621 gtcaggaaaa actgttcacc tatcttgatc aaggtgtgat ttatcgtatg ggagaaccta
    16681 atttgtcacg ccggatcaaa acacgtctct tttttgctac gactgaagag attactagtc
    16741 atttcttaac aactttcatt cgaagaatac ctatccgaat cgaactacct gcacttaatc
    16801 agcgtagtcg taatgaacgg ctagaactga tttactcttt ttttctcgaa gaacaaagaa
    16861 aaatggggag ccctttgtca gtcagtggac cagctttgtc acttttaaca agtcagaaat
    16921 tatccggaaa cataggagag ctgaaaaata tcgtcaaagt ttcagcagca aaagcatatg
    16981 ctgaacagcg tgaacagcct gaaatccatg tcacgatcca tcatttgcaa aaagaactac
    17041 ttgcgcagcc tattactaaa aatacagcac agcaaggaat ctatattacg caagaccaga
    17101 ccttagagca attaatggag aagcaacagc ccgaacagca gcgaatcgtt aaaagttttg
    17161 agcaaatcct aattgatttc aagaaaaata actgtctgct atattcttgc gaaggaaaat
    17221 taaagcaaga agtgattcaa ttatttgatt ttttgctgtt tgagaccagt agacaagaaa
    17281 aacacgagct gctggtttat tggacacaat ctatccgaga gacctttcga caaatggaaa
    17341 ctgcctatca aatcaaattc gatggaaaca gtgtctatgc cttgagttac tatctctatc
    17401 agcgaagcac tatcaaatgg ttaccggaag aacatgatat tatccaacta atcaaagaat
    17461 tagaacaaca agtttctcag tcttatccat ctagttatca ctatgtccaa cgaattctgg
    17521 aattaagtaa gatcaaatta gatattgaag acacaagtat ggatcggatt ttactgacga
    17581 tctatctaaa aaaagcgaaa tggtcaaaag aaaaacgact gccaaaagcg atgattgctg
    17641 ctcacggtta tgcaactgct agcagtatgg caaatgtagt aaatcgttta ttacaagagg
    17701 atctatttga atcatttgat atgccactag atgtcacacc acagcagatt gcttcagaaa
    17761 tccttgatta ttgcgaaaac agcgatgttt caaatggatt gatcatatta gtagacatgg
    17821 gatctctgaa agaaatccat cagtttttta aaaaacagtt atccgtaccg cttttgattt
    17881 tgaataatgt gacgacacct ttagcaatca cagtagggga atgcctgcaa aaaaatgtat
    17941 cattagaaga gatagcagaa gagactgttc gacagatcca gccagaatgg cgactgctct
    18001 atccagagga aaacaaaccc aaagcactca ttactacttg cttcactgga atcggtacgg
    18061 cggtccactt aagtgaactg ctagaaaaaa gcttacctac ggcttgtcag ttgaaaatca
    18121 tcccatatga atatcagcaa ttaaaagata agaaaaacag tgacccatta ttttccatat
    18181 acgaagtcgt aggaatgatt ggcaccacag atcctgatat caccaatatc gactatcttt
    18241 cattagaaga cctcatctcg ggggaaaaga tgtctgtact agctgaatgg ttggacacta
    18301 ctatgaatgc ttcagagaag gaaatattca atcagcgcat cattcgtaat ttttcattgg
    18361 aaaaagtact ggattccgtg actattctag atacagagaa ggtgatggga gaaatcgatc
    18421 attttatgag agaattagag gtgcagttga atcgaagcat cagcaatccc aaaaaattag
    18481 cgttgtatgt ccatgtgagc tgtctcattg aacgattgat ccgacaaatg ccaatcgaaa
    18541 cctatcaagg attagagaaa tggaagcagt gtcaaaaaga agggttatct gctattaaat
    18601 ctgcgtttag tgtcattgaa gagcattata gtgtcatgat ccccaattct gagattgcct
    18661 atatctatga tattttgagt gaagatacag agtttctctc gatagaagaa gaattttgaa
    18721 cataagcaag ttgttgccac taattttggc atgcaacttg cttttttagt atgtgtaagt
    18781 gaaaagagga gatgaaaaag tggatagaga aattatctta gcttcccatg gaaaatttgc
    18841 ttcagggata ttagattcat tagagctgat ctatgggaaa aatcattcta ttactgtatt
    18901 cgattgctat actgatgaga attttgattt agcggaaaca gtaaggcagc tttttatgag
    18961 gtacaaagat aaagaaatca ttgttctgac ggatcttttt ggcggaagtg tcaacaacga
    19021 attcttacaa tatatcaatc gggatcatgt ctatctcgtt gccggattga atttgccact
    19081 attgattgag ctagtatctc ggttggacgc tgaagtagag acaactgttt tgatccagca
    19141 ggtgctaact gattcaaaaa aaatgatcca gttttgtaac gagcggctca atcagaaaat
    19201 agaagaagac gaattttagg aggaagaaaa atgattgtat tgacaagagt cgaccatcgg
    19261 ttattacacg gacaagtagc tttttcatgg acacaaacaa taggcgcaga ctgtattttg
    19321 attgctaatg atgatgtacc tacgaatgag atacgaaaga caacgatcaa attagccaag
    19381 ccacaaggag tcaaactagt gatcaaatca attgatgact cgattgccgc gttgaaaagc
    19441 ggggtgacag ataaatataa gttattcatc gtagtggaat cgatcgagga tgcatataag
    19501 ctggcagaag cgtatccaga aatcaaaacc atcaacttag gagggatcaa ggcaaaacct
    19561 ggtactcgca gcatcggaaa agcggtaaat attttaccag aagaggagga acgcttgaaa
    19621 cgattaattg acaaacaaat agaagtagaa atccgtcaag taccagcaga taaaaaagtg
    19681 gatgtaagag aagttttgta agaaaggaag aacctcaatg attgtacaag cagtcttatt
    19741 aggaattgtt gcatttgtcg cacaatcaga atatgcacta gggacatcgt tgctttctcg
    19801 tccgattgtg acggggctgt ttacaggaat cgtgttaggt gatgtaaaaa ctgggatcat
    19861 tatgggcgca actttagagt tagcatttat cggatcgttc tcagtaggtg cttcgattcc
    19921 acctgatgtt gtgaccggtg ggattttagg gacggctttt gctattacag ctggagccgg
    19981 tacagaaacc gcgcttttac taggtttgcc gattgctacg ttgacattga tattgaaaaa
    20041 tatttactta gggcttttga ttccaattat gaaccataaa gcagacaatt atgcagaaga
    20101 aggaaattat aaaggagtcg agcatatgca cttgcttgcc ggtatcggtc tttccttgat
    20161 gcttggtgtt gtggtaatgg tttcttatat ggtgggaagc aatacgatcg gaaatctatt
    20221 aaatatgatt cctgacttcg ttcaaaaggg gttgtcagta gctacagggt tgattccagc
    20281 tcttggtttt gcgatgcttg ctcgtttgct gatcaacaaa caagttgcac catatttttt
    20341 cttaggtttt gctatagctt cttacttaga gattcctgtg accggagtag ctatttttgg
    20401 tgcgatttta gcagttgtgg tagtcaatat tatgaatgtc cgtcaactac cacttcaaac
    20461 aactgcaggg gaggtagaag aagatgaaga attctgagat caaaaacagc aaaataacga
    20521 aaaaagagct aaatcacgta ttttggcgtt cttttcaaat ggagttttct tggaattatg
    20581 aacgtcagat gaatatggct tatgtttatg caatgattcc gattttgaaa aagttatatc
    20641 agtcgaaaga agaaatggca gcagctttga aacggcatct agagtttttt aacacgaccc
    20701 cacatatcgt tacactgatt ttaggaatca cggctgcaat ggaggaagaa aataaggatg
    20761 atcctgaatt tgatgtgact gcgatcgata gtatcaaaac atctttgatg ggacctcttg
    20821 cagggttagg agattctttc ttttggggaa ctttgcgatt gattgccaca ggtgtgggta
    20881 cctctttagc catgcaagga aatattttag gccctatctt gttcattttg attttcaata
    20941 ttccgcacat tctgtttcgc tatctagcaa caggttgggg atatcgctta ggaacgggtt
    21001 ttttgaaaaa gatccaggca aacggtatga tggaaagttt gacattaggc gcgtctatta
    21061 ttggactgat ggtcgttgga gcaatgactg ccacgatgat caccatcaat attcctttaa
    21121 aaatcggttc aggagaaaat gctgtgactg ttcaaagtat ttttgatgat atcgttccaa
    21181 atatcttatg tcttggagca tttggcatag tgttttatct attgaagaaa gaagtgaaac
    21241 cattaacgat cttgatcgga ctagctttct ttggaatttt tggttcgtgg atagggattt
    21301 tttaaaaaag gagcagagaa aatgtttacg atgcaagatt atatctatga agaaaaggaa
    21361 gtactaagta cgatcttgaa aaagaatgat ttttcgacaa gagaacatat gaaaaaaaca
    21421 gtgaatttac tgatcttagc aacgggttca tcttataacg cttgtttagc agcgaagcca
    21481 gcattggaat cttatggtga tcttactgtc gatattcaag aaccttttta ttttttcaat
    21541 tatggaaaac tttctccttc aatcgatact gtgattgctg tgtcacaaag cgggaaaagt
    21601 gcttctactg ttgaagcagt aaaaatgata caaaaacaag ggttacctgt tgttgctatc
    21661 acaaatgacg tgcaaagtcc acttgcttta gaagctgcac aaattattga tttaggtgcc
    21721 ggggtagaat cagtaggctt cgttacaaaa ggctattctg cgactgtttt acagttacta
    21781 ctcttaggga taggaattgg aataagtaaa aataaaatca gcaaaaaaat agaacaagac
    21841 tacatgcaac agttgcgcaa aataatcaac catttaccag cgattattca aaaaacggaa
    21901 gaattttttg atggatatca aagtttattt cgtttagcac agcgtttcgt caccattgga
    21961 tatggtccaa actggggaac agctaaagaa gcagagacga aattgactga aacaatccgc
    22021 gttccgtcac aaggatttga gttagaagct tatatgcacg gcccatacct ggaggcagat
    22081 gcttcgcatt tgctattctt tattgaaggg gaaagtgtaa ataaggaacg ttcgcaaaag
    22141 ttgcaaagat atatgagtcg gtacgttgga gagactctca ccattaccac aaaaaaagca
    22201 agaaacgaga aaacacttgg attagcaata gaatgtgatg aatatctgtc tgtattagcg
    22261 cttgtcgttc cgtttcaact attcgcttat aaaaccgcag tagctaaagg tattgacttg
    22321 aacaaaaaga tttttgaaga ctttgataca gtcttgaaaa gtaaattata agaggtggaa
    22381 atcatgctga aattcaatga agaagaacaa atcaaagcaa taaaaggcgc actagcttta
    22441 cgtccgcaag tagaagaaat catcgataaa ctttatgcgg aaaaatttga cgctgtctat
    22501 tatctaggaa tcggtggaac gtatgcttcc tcgatgcaag ctgtcactta tatgaatggg
    22561 aaaagcaact tgcctgtctt tgtacagcat gctgcagaat attacactac tggaaacaaa
    22621 agattaacaa aagattctat cgttattttg tcctctgtta ccggtacaac tcaagaagta
    22681 gtgaaagcag tagaacaaat caagaaagta ggagcgacac tgattggatt tatcgataaa
    22741 gcgaacagta agttgtctca gctatgcgat tttgtggtca cttatccagc tcctggaaca
    22801 gaacaaatca aattttttat ggcagcagat cgattgatgt atttgaatgg agaattcgaa
    22861 gcttattcag aatactatga acagctcgaa cgttatttac caacagggtt agtggaagca
    22921 gagaaaaaag cagatgcatt tggcttatca tttgcagaaa aacatcgacg tgatagtatg
    22981 cattatttta ttggagcagg gaatcaatgg ggagctgttt attcatatgc catgtgttac
    23041 tgggaagaac aaagctggct tccatcgaaa tctatccatg ctgccgagtt tcttcacggg
    23101 acgttagaga tcgtagaaga aacaacccca gtcacattat ttttaggcga agatgaacag
    23161 agagtgttat ctgaacgtgt ggctaaactt ttgccaaaaa tttgcagcaa ttatacattg
    23221 atagacacaa aagactatcc agtagaaggt atcagtgaaa aatatcgcgg acgtgttttg
    23281 tctttcttat tgatgcatgt agtgacacaa cgcatcgatg ctcatgtgga gcagttgaac
    23341 tgccatccat tagagatacg ccgctactat cgtcagtttg attattagga tagactggat
    23401 aaaaagaata taaaaaataa agagagcagc gataactgct ccctttattt ttttaaaata
    23461 ttggcatcaa ttacgttata aaacacattt tttgcattat ctacagttca acttataatc
    23521 tgtttaatgg gacatgtggg tgccaaccat tttttgttat gcaatagtac tatttagttg
    23581 ttgcatggat ccgtagtaaa agtccaagta caacgagttg cgccagttta catagcgttt
    23641 tgtatccaga atgttgacta aaacaaaagt tcctatgtca accgtcaaca gagacaatct
    23701 gtccgttgca ggacctgaca gatttagaaa tgttcaatct gtctcttatg tattttaaat
    23761 caatactcac taggagaagt ttcctaacct cctaatagta atgctggtag taacattgtc
    23821 aaaactgctt cctcttagga acaagcaaag cataacgagt gtaaaaataa aatgtagact
    23881 attgtaacat atcattgatt atttcaagta aaaacgataa aagaagtaaa caaacactta
    23941 aaaacaatgg aaattagtcc caactttaag aaatgtatac tataatggaa attatcaatt
    24001 atttatttgg aggaatatcc atgaaacaaa aaatcgcagt tcttggtcct ggatcttggg
    24061 gcactgcttt agcacaaaca ttggcagaaa acgggcatga tgtccgtatt tggggaaatg
    24121 tacctgaaca gattgatgaa atcaataagt atcatacaaa tcagcatttt ttacctgatt
    24181 tgacgatccc agaatcgatt atcggatata aagaattggc agaagcagtg gataacgcag
    24241 atgctatttt atttgttgtt ccaacaaaag cgattcgttc agtcgcacag gaacttattg
    24301 gtaaaatgaa tactaagcca atcattatac atgcaagtaa aggactagaa caagatacgc
    24361 ataaacgaat ctcagaagtt ttggctgaag aaattccaga agaaaaaaga caggcaattg
    24421 ttgtattatc tggtcctagc catgctgagg aagtggcagt acatgatatc acaacaatta
    24481 cagctgcaag tattgatcaa aaagcagcag cctatgtaca aaaactgttc atgaatgatt
    24541 atttcaggat ttatacaaat aatgatgtga ttggcgtaga aactggtgca gcactgaaaa
    24601 atatcattgc aattggtgcc ggcgctatcc atggattagg attcggagat gatgcaaaag
    24661 cagcaatcat gactcgagga ttggcagaaa tcagccgtct tggtgtcgcg atgggtgcca
    24721 atcctctgac atttatcggt ttgagtggtg tgggggactt gatcgttact tgtacaagtg
    24781 ttcattctcg caattggcgt gccggtaact tgttaggaca gggacataag ctggaagaag
    24841 ttctagaaaa catgggaatg gtcgttgagg gtgtggcaac gacaaaagca gcagtagagc
    24901 tagctcaaca gctaggtgtt gagatgccaa ttactcaaac aatctataat gttttgtata
    24961 atggagaaga tatcaaaaaa gcagcaaaag aaatcatgtt acgtgacgga aaaatggaga
    25021 atgaattttc tcttcgttaa atagatgatc aatagttaat cgtcgtttag gaggaacagc
    25081 aagtgaaggt caaaaaagca gtcattcctg ccgctgggct aggaacacgt tttttgccgg
    25141 caacaaaagc catggcaaaa gaaatgcttc caatcgtgga taaaccaacg atccagttta
    25201 ttgtggaaga agcattggat tcgggaatcg aagacatctt gatcgtaacg ggaaaagaaa
    25261 aaagacccat tgaagatcac tttgatgcaa atatcgaatt ggaaaacaat ttgcgggaaa
    25321 aggaaaaaac agaattactc gctttagttg aagaaacgac tcaagtcaat cttcacttca
    25381 tccgtcaatc tcatccaaga ggactggggg atgccgtgct tcaagcaaaa gcatttatag
    25441 gaaatgaacc gtttgtcgtc atgctggggg atgatttgat gaaagatgat atcccattga
    25501 ctcaacaatt gatgatggat tatgaagaaa cacaagcttc cgctgttgcc gtcatgcgtg
    25561 taccagaaaa tgaaacatct aaatacggaa ttattgatcc taaaacagat agcgaaaagg
    25621 gtcgttgcag agtaaaaggg tttgtagaaa aacctgaaat gggaaaagca ccaagtaatt
    25681 tagccattat cggacgttat ctactgactc ctaaaatatt tgagatatta gaaactcaag
    25741 aaccaggagc aggaaacgag atacagctaa cggatgcact tcagactctt aaccagacag
    25801 aggcggtcta tgcgagagag tttaaaggaa aaagatacga cgtaggcgat aagttaggat
    25861 atatgaagac aaatattgaa tatggacttc aacattcgga aattgggact tcattaagtg
    25921 catatattat ccagttgagt aaaaagctaa gaacagaaag ttaactggaa ataagtggcc
    25981 tgctgagaat gaaaggctat ttgttattaa agcggcagct tgtgcgtcat ttgcgcaact
    26041 gtcgttttag ttttttgact ctagatcagt tgagtcgtgg aaaacgaaag atagaaaaag
    26101 actttcgtga atagattcag aaaatattat gctaatggtt taattttttt ttaagttgta
    26161 taaaagaaaa tgattacata agagaaatga aagccaagcc agaagattca tagtagaata
    26221 aaaaggcgaa ttttttaatt ataaaaaaag gtgtgtgttc actatgggaa aattagtcgc
    26281 tgttgcacag tcgctgcttg atatactcaa gtggctgatg gaaggaagca attggatatt
    26341 tgtgttgcca ctattgctta ttagtctatt tatcatgttt cgcatggtca gaaaaaaaga
    26401 aacgtttctg atttttatct ggtatgcagc atccgttggg gttgctacct tgatggttga
    26461 attggcgtta ggtagttggg aagcttgcgt atttgcttta ttttttgctg tactgtatct
    26521 aatcatttat atcccatata tgaaaagaaa gtctcggata ggcagaaaag gtagacatac
    26581 aactactttt tctagtacag attctctcga acaaagtaga agaacgagaa gaagacggta
    26641 atatttagta ggtatacgca aaaaaagata aaataatgga taaactattg agatgagaga
    26701 tttatttaat tggtggaaaa aagctttttt cacttatata aaagaatctt ttttaaagga
    26761 atgcttgttg tatcgggata agccattcct ttttttatgt tatttatata tatataaatc
    26821 ataccaatgt ttttttgtat tttcttgctt gcacttggat aagagattac ttacaataaa
    26881 ttcagataga aacagctgaa taaaaaaagg ctccctgacc cttagttggc gctaagaatc
    26941 agggaatgac cagtcacaca catacaccct gaccgatcat tttgccatag tcaatgcagt
    27001 gcatcgacct aatttattgt acctaatcta gggtgaaaat tctaccccta tgtagatatt
    27061 ttccatggtc tgcacgttgt tttatttgct atgtcaaggt attgggcgta ttcgttcaat
    27121 gccttttttg tgtttgaaac agcaaagacc acatgtgtta ctgtctaact actgtttttt
    27181 tatgtacttc cttagattag gcagctgttt ctattcatgt caagtatagc taggcgggag
    27241 agataaatga gtttatgtta ttttgactat tatcaccaat tggaagagaa attaaggata
    27301 gaagagccgc tgatgcttca agctggcact tggatcgcga aacaaattaa tcaaggtggt
    27361 agattgcaga tatatgctag ccgcacacta agtggtgtag catttgaatt ttgggatcaa
    27421 accccggatc ttgtaccaag tatattgatt gaaaatccag ccggaggtgt ctatgaaacg
    27481 ctagaaggtg caggacaggc aattattgat cagctttctg tcaaatcaga agacattttt
    27541 cttcttcttt cgaatgaagg aagaaacccg gcaatcatcg aactagctca gtggatcaaa
    27601 gaaaatggtc atccattaat cattgttact ggatttgatt tgtcacgaag tatcaaatcg
    27661 caacatagca gtggtttgcg attatttgaa tttgctgatt tggttttgga caaccacgcc
    27721 aatttacatg atgcagtctt gactttgcca aatctagatc cagcgatctg cggaacagcc
    27781 tctcttgcaa cgatcttttt gctgcagcaa gttttgtatt ttacagcaaa acagctagtc
    27841 caggacacaa gtggaaaata gttatccaca tgttgacaaa gttccttaaa aaaataatgt
    27901 tccacataaa aatggagcgt ttgaaaaaaa gaaaacgatt attaatgagg attaacaacg
    27961 actacttttt ttgttttagt tattttaaga tggatcatct ttgattttaa gcttgttttg
    28021 tttaaagttt gttattttta taagttgttt cacagttatg agaaatgagt tataagaata
    28081 tgtagtgtca gaaaaataaa aagtagtgga aaaagagaca ag
//
LOCUS       DACDUU010000033        28113 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.033, whole genome shotgun sequence.
ACCESSION   DACDUU010000033 DACDUU010000000
VERSION     DACDUU010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.033"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            2..74
                     /locus_tag="IWL30_001456"
     tRNA            2..74
                     /locus_tag="IWL30_001456"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            78..150
                     /locus_tag="IWL30_001457"
     tRNA            78..150
                     /locus_tag="IWL30_001457"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Phe,seq:gaa)
     gene            155..225
                     /locus_tag="IWL30_001458"
     tRNA            155..225
                     /locus_tag="IWL30_001458"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Gly,seq:tcc)
     gene            238..311
                     /locus_tag="IWL30_001459"
     tRNA            238..311
                     /locus_tag="IWL30_001459"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:gat)
     gene            323..394
                     /locus_tag="IWL30_001460"
     tRNA            323..394
                     /locus_tag="IWL30_001460"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Glu,seq:ttc)
     gene            660..1853
                     /locus_tag="IWL30_001461"
     CDS             660..1853
                     /locus_tag="IWL30_001461"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAP9706231.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1983..3455
                     /locus_tag="IWL30_001462"
     CDS             1983..3455
                     /locus_tag="IWL30_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9706232.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3538..4326
                     /locus_tag="IWL30_001463"
     CDS             3538..4326
                     /locus_tag="IWL30_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAP9706233.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4393..5061)
                     /locus_tag="IWL30_001464"
     CDS             complement(4393..5061)
                     /locus_tag="IWL30_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP9706234.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5176..6888
                     /locus_tag="IWL30_001465"
     CDS             5176..6888
                     /locus_tag="IWL30_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9706235.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDLDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6891..8660
                     /locus_tag="IWL30_001466"
     CDS             6891..8660
                     /locus_tag="IWL30_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9706236.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(8865..9431)
                     /locus_tag="IWL30_001467"
     CDS             complement(8865..9431)
                     /locus_tag="IWL30_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9706237.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(9431..10387)
                     /locus_tag="IWL30_001468"
     CDS             complement(9431..10387)
                     /locus_tag="IWL30_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAP9706238.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(10387..11043)
                     /locus_tag="IWL30_001469"
     CDS             complement(10387..11043)
                     /locus_tag="IWL30_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP9706239.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            11413..13431
                     /locus_tag="IWL30_001470"
     CDS             11413..13431
                     /locus_tag="IWL30_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9706240.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            13858..16272
                     /locus_tag="IWL30_001471"
     CDS             13858..16272
                     /locus_tag="IWL30_001471"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAP9706241.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            16620..18162
                     /locus_tag="IWL30_001472"
                     /pseudo
     CDS             16620..18162
                     /locus_tag="IWL30_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
     gene            complement(18543..20327)
                     /locus_tag="IWL30_001473"
     CDS             complement(18543..20327)
                     /locus_tag="IWL30_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9706242.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNFAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(20342..22063)
                     /locus_tag="IWL30_001474"
     CDS             complement(20342..22063)
                     /locus_tag="IWL30_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9706243.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLQIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            22163..22924
                     /locus_tag="IWL30_001475"
     CDS             22163..22924
                     /locus_tag="IWL30_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9706244.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKK
                     IRERKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            23891..24159
                     /locus_tag="IWL30_001476"
                     /pseudo
     CDS             23891..24159
                     /locus_tag="IWL30_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            24831..27509
                     /locus_tag="IWL30_001477"
                     /pseudo
     CDS             24831..27509
                     /locus_tag="IWL30_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         9013 a         4961 c         5945 g         8194 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgtttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
      121 gtgtcggcag ttcgattctg tcccgcgcca ttatgcgggt gtagtttagt ggtaaaacca
      181 cagccttcca agctgttgtc gcgagttcga ttctcgtcac ccgcttttag tttttatggg
      241 cctatagctc agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc
      301 atttaggccc atttatttta ttggcccgtt ggtcaagcgg ttaagacacc gccctttcac
      361 ggcggtaaca cgggttcgaa tcccgtacgg gtcatggaag atactttttg agtatctttt
      421 tctttttatc tgaaagaatg attttaaaaa gcttagcaga gttatcttta tggatgattg
      481 tgctaagctt ttttggacag ttctaatgta aaatattttt atatttttta agcaaaaaaa
      541 tgaaaaaata cgagcagaaa actgttttat ctgctatact tggttcgttg caagttcccg
      601 aaaggatccg caggacggag atgccttgta accgaattta actaggggga atcattataa
      661 tggtagaaag acacttattt acatcagaat ctgtttctga aggacatcca gataaaattg
      721 ctgaccaaat cagtgatgca atcttggatg caattttaaa acaagatcca acagcacgag
      781 tagcgtgtga aacgtctgta acaactggtc ttgttttagt atttggagaa atatctacga
      841 cggcatatgt agatattcaa aaagtcgtac gggaaacaat aaaagaaatt ggttatacac
      901 gtgcaaaatt cggatttgat ggagatacag cagctgtatt ggttgcgatt gatgaacaat
      961 ctcctgatat tgctcaagga gttgatgaag cacttgagat ccgggatgaa gataaaaaag
     1021 acgtattaga tgagattggt gctggtgacc aaggtttaat gtttgggttt gctgtagatg
     1081 aaacaccaga attgatgcct ttaccaatcg ctttgagtca tcgtctggta cgacgcttgg
     1141 cagatttgcg taaatcaaat gaattaacgt atttacgtcc agatgcaaaa tctcaagtaa
     1201 cggttgaata tgatgatcaa ggacaaccgg aacgcgtaga tacaatcgtt atttcaacac
     1261 agcatgatga tgcagtagat aatgaaacaa ttcgtcatga tgtcatcgaa aaagtagtga
     1321 aggaagttat tccagctgaa ttattagatg atcaaacgaa atattatatc aatccgactg
     1381 gccgatttgt cattggtggt cctcaagggg atgccggatt aacaggaaga aaaatcattg
     1441 ttgatacgta tggcggttat gctcgtcatg gtggcggtgc tttttctggt aaagatgcga
     1501 caaaagttga ccgttctgcc agctatgctg cacgttatat tgctaaaaac attgttgcag
     1561 cagggcttgc tcgcaaagca gaagtacagc tagcttatgc gattggtgtt gctcaacctg
     1621 tttcgatctc aatcaatact ttcggtactg gaacggttcc ggaagaagaa ctgattgctg
     1681 cagtgagaga aaacttcgat cttcggccag caggaatcat cgagatgctt gatttgcgtc
     1741 gtccgattta caaacaaaca gctgcttacg gtcattttgg tcgtacagat gtagatttgc
     1801 cttgggaaca tacggacaaa gtagatgcgt tgaaagcaag tttagcaaaa taagttatat
     1861 tcagctggaa cgatcgccaa gtggtggttg ttccagcttt ttttatggca gttaacctct
     1921 tgaaaaagat agtaattatt tatctatttt tagatcctgt cataatgaaa gggaggaata
     1981 agatgaaaaa agaaacaaat gttactttgg tcacagtgag tattttcatt gcaacgttca
     2041 tgacggctat tgaaggtaca atcgtaacaa ccgctatgcc aacgattgtc ggttcattac
     2101 atggaatgga aatcatgaac tgggtatttt cgatttattt gctgacaaac gctatgctta
     2161 ctccgattta cggaaaactt gcggataaag tgggacggaa accaatcttt atttttggga
     2221 cattattgtt tattatcggt tcgttatttt gcggtttatc tcaaagcatg attgggttga
     2281 ttgctgctcg tgcgattcaa gggatgggtg ctggggcaat gatgccagta gctttgacga
     2341 tcatcggcga tctttattct attgaaaagc gagcaaaagt tttaggtttg aatagttcag
     2401 cctgggggat tgccagtgtt tttggaccgc ttgcgggagg attcatcgtt gaaacgatca
     2461 gctggcactg gatctttttt atcaatgtgc cgatcggaat tctattaatt attttgattt
     2521 ggctatattt aaatgaacca aaaatcatac atgagtctaa accaatggat atttttggta
     2581 gttttagttt gatggccatg cttgtctcat tgctgttagg gttccaatta ttaggagacg
     2641 aaggaatgag tccgcttgtt tctgctttgc tgctaggtag tatcgtaatg ttaggtattt
     2701 ttgtttttgc cgaaaaaaga gctaaagatc ctgtcatcat gatgcattta ttcaaaaatc
     2761 cactatttgt tgtggtcaac ttggctgcag cattggctag tggttttttg atgggaatcg
     2821 atgtttatat ccctatgtgg atgcaaggtg tattagggct gaatgcagca attggtgggc
     2881 tggtattagc accattatca ctagtctgga tggtcggttc tttccttgca agcaagtgga
     2941 tgactcaaat gaaaactcat tgggtcttga gaattggact actaatcgca gctattgggg
     3001 gcgcttggtt gtatcttatg cctttcagaa gtaactattt atggtttttt gctatatcaa
     3061 cggtcatcgg tataggctta ggtgtgacca tgactacctc gactgtcagt gcacaaaata
     3121 gtgtatctcg tgaccagctt ggtgtagcaa cttcttttaa tacgttagtt cgaacaattg
     3181 gtcaaacagt catggtttct atttttggtt tattgataaa tggggtaact aaacgagaac
     3241 ttgcagcagc aaatttaaca aatgatgcag atatcatgaa taaactggtg aatccacaaa
     3301 cggctaaata tattgatgcg gatattttgt taccattaag aaaaattctt tatgatgggt
     3361 tacattccgt ttatctagtt ggcttgttac tcgtgatcat tgctttggtc ttaacgcttt
     3421 tcgttcatga aaaaagtggt aaaataaaac agtagtttcg gttaagatat gtacgtagcc
     3481 gaatcattca aatttaaagt tattcaaaaa taacttctta ctaaggaagg cgaaaatatg
     3541 gcatttattc aagcaaatat ttattcgaat gtgttagaaa tggaagtcaa tctaaatgtg
     3601 atactaccgc aaacaacaaa aaaggtgatt ggcaccagtt cagagcaaga gacaacggat
     3661 atacctgttt tatatttact tcatggaatg ggtggcaatc atagtgtatg ggaaagacgt
     3721 acgtcgattg aacgttatgt agcaaactac ggattagctg tcatcatgcc atctactgat
     3781 cttggctggt acacagatac agtttatgac atgaagtatt ggacattcat tgcagaagag
     3841 ctacctgaga tttgtcatga attattcccg caattaacaa gaaaaagaga aaaaacatat
     3901 gccgctggat tatcgatggg cggatatggc gcgttaaaac taggccttgc aaaatcagaa
     3961 aatttcgcag cagtaatctc tctttcaggc gctgtttctg taggaaatcg aatggatgat
     4021 cttttgatgg ttcgtaaagc atctttttgg gaagggatct ttggaccatt ggatcaaatc
     4081 gaaggttcaa aaaatgatcc gcaatactta ttgaaagaac tagtaaaaag cggaaaacca
     4141 gttccaagat tttatatggc ttgtggggaa tctgattttc tttatcaggc aaatcaggaa
     4201 atggtatctg ctatgcagga acaaggcttg gcggtcacct ttgaacaagg acctggcgat
     4261 cataactgga tcttctggga ccaatggatt caacgtgcat tggcgtggct aatggaagaa
     4321 aaataaaaaa agagtcttaa aaagatagct cctatagaga tcacaaacat agtgatctct
     4381 ataggagctt ttttactgct cgttttctct tcttttattt tgttggcata agccttcaat
     4441 ataggtttgt gcactcgtta gaaagagtgc tttttcattc aattctgcat gggactgcat
     4501 actttcttta aatccctcta attgattttc aactacataa ttacgcataa atagaactgt
     4561 ttctttcaaa aattcatttc cgtccataat tttttgtcga agctgctgtt catgctgctg
     4621 atccattaac tgtccaatca ataaattgtg gagagaggca atggcctgat gactttgggt
     4681 gactgaaaat ccttcacgaa caagtaattg gatcatttgc tccaaatgtt ctaatcgact
     4741 cggaaaagcg ggcacagtat tgatttctaa ttctgcccca caagggaaac gtgtataaag
     4801 gtcatagtaa ttttccatat actgcagcaa ctgttcattc caaggcaaag ttggatctgg
     4861 aagcgctaat tcttcttcca ttgtttcagc cattatttgc aagagctgtt gtttgttttt
     4921 gaaataccaa tataaagcag gtgcttgaac atgaattctg tttgcgatgt ttcgcatgga
     4981 aagctgcgaa agagcttgtg tttcagccag gatatcaaag gcggcctgaa tgatgattgc
     5041 ttgagaaagc tttatttcca ttttatcaac ctcgtgtatt gcattatctt tttaaacatg
     5101 gtaaattgtt agtcggtcta attttacagt gttaaattag tcctgtcaat ttaaacaaag
     5161 gggtgtaatc aacgaatgaa attaatctta cgacatgcga aaaaatataa agtagctgtt
     5221 tttatttcct tgctgtccgt agcagtcatg gttgcagcag cattatggca acctaagtta
     5281 ctacagcaag tattggaagc tattattaca gaagataatg atgaaatgaa aacaattggt
     5341 atttacctga tcagtctggc tttgctagga ttagccgcag gtgtaacgaa cacgattttc
     5401 tctgcaaaag tagctcaagg cgtaagtgca gatatccgag aagaagcatt tcgaaagatc
     5461 cagacatttt catttggaaa tatcgaacag ttctctgctg gaaatcttgt tgttcgtcta
     5521 acaaatgata tcacgcaaat ccaaaattta gtgatgattt ctttgcaatc actttttcga
     5581 attccattct tatttattgg tgccttcatt ttagctatga tcacgatgcc gcaattatgg
     5641 tggatcatcg tagcgcttat cgttacggta tttctgatta cggcactttc atttacaagg
     5701 atgggcaagc acttcatgat cattcaaaaa ctgattgatc gtgtgaatag cattgcaaaa
     5761 gaaaacttac tagggattcg tgtagtgaaa tcttttgtac aagaagaaaa ccaattgaag
     5821 tcattcagca aagtaagtga ggacttaaca aaacacaaca tcatagtcgg tacattattt
     5881 tctgtgatga tcccgtcctt catgctggct gccaatttgg ctgtggttgg ggcgatcttt
     5941 tttgtgagcg atcttgttaa agatgatcct acgttaattg gtggaattgc ttcattcatg
     6001 aactacttga tgcagattat gatggcaatc attattggcg gaatgatgat gatgatgact
     6061 tcaagagcag ccgtatcgct gaagcggatt gcggaaattt tagatgctga accagatctt
     6121 acttatttag acgttccaga acaagagttg acgggttctg tcacttttga ccatgtttct
     6181 tttcgttatc ctggtgagga tacagatacg cttaaagata tcagcttttc tatcaaacct
     6241 ggagaaatgg ttggtatcgt cggggcgact ggtgccggga agtcaacact agcacaattg
     6301 atccctcgtc tatttgatcc gacagaaggc aaagttgaag taggtggtgt agatcttcgt
     6361 caagtaaatg aaaagagttt aagacaaacg gtttcctttg ttttgcaaaa agcaatttta
     6421 ttctcaggaa cgattgctca aaacttgcgc caagggaaaa agaatgcaac agaaaaagag
     6481 atggaacatg cttcttctat tgctcaagca aaagaattca tcgaaaagtt atctgatcgt
     6541 tacgaagcac caatagaaga acgtagcagt aacttttctg gtggtcaaaa gcaacgttta
     6601 tccattactc gtggcgttat cggtaatcca aagatcttga tcctagatga cagtacgagt
     6661 gcactggatg ctcgctctga aaagctggtc cgagaagcac tcgatctaga tttgaaggga
     6721 acaacgacga ttgtcatcgc acaaaaaatt gcttctgttg tcaaagcaga ccgcatctta
     6781 gtattggatg aaggcagatt agtcggcgaa ggaacccatg aggaattggt agctggaaac
     6841 cgtgtctatc aggaaatctt tgaaactcaa aaaggaacgg aggagtaagt atgactgatc
     6901 taataaaagc aagtaaattt ttctatcact atttgaagcg gtacaaactc tcctttttct
     6961 ttatctttat cacagtgatc attgctacct atctacaagt aaaggcacct caatacatcg
     7021 gggaagcttt tcaagaatta gctaattacg taggaggctt gatgcaaggc gtagacgata
     7081 aaagcaaatt tgtttctgta atatggaaac tcttgctgtt ttatgtacta gctagtgcag
     7141 ccaactttat ctatagtatt ttgttcacac aagtagtagg gaagtcaacg aaccgaatgc
     7201 gtatcggtct attcaacaaa ttggaaaaat tgacgatccg tttctttgat tctcatcaag
     7261 acggtgaaat tttaagtcgt tttaccagtg acttagataa tattcaaaat agtttgaacc
     7321 aagctttgct tcaagtcatc acgaatgctg ttttacttgt cgggatcttg atcatgatgt
     7381 tccgccaaaa tgtagagtta gcttgggcta caattgcttc cactccagta gccgttttga
     7441 tagctgtctt gattatccgc aaagcacgga aatatgtgga tcttcagcaa gatgaagtgg
     7501 ggaaactaaa tggttatatg gatgaaaaaa tcagcggaca acgtgtcatc atcacaaatg
     7561 gcttacaaga agaaacgatt gaaggattct tagagcataa tcaaagtgtt cgcgaagcaa
     7621 ccttcaaagg acaagcttat tctggtctac tatttccgat gatgcaagga atgtcactgg
     7681 tcaatacagc gatagttatc tttttcggcg gctggttagc attaaatggt gatttggaac
     7741 gttcggtggc attaggctta gttgtgacat tcgtccagta ttcacaacaa tattaccaac
     7801 cactgatgca gatttcatct ggatacagca tgatccaatt agcgattaca ggggcaagac
     7861 gattgaatga aatatttgat gaaaaagatg aagtcaaacc agaaaactgt aaagcatttg
     7921 atggtgttga aaaaggtctt gcgttgcagc acgtagattt tggttattct ccagaaaaac
     7981 tagtcttgaa agatgtctcg atcaatgtgg acaaaggaga aatggttgct cttgttggtc
     8041 caacaggatc ggggaaaacg acgatcatga atctgctaaa ccgtttttac gatgtcaatg
     8101 atggttcagt agtcatcgat ggaatagata tccgagatta tgatcttgat gcattacgta
     8161 gacatgtagg aatcgttttg caagaatctg tattgttctc cggaacgatc aaagacaata
     8221 tcacattcgg caaaccggac gcttcagatg aagaagtcat caatgcagca aaacaagcga
     8281 atattcatga cttcatcatg acgttagaaa aaggttatga tacagagata tccgaagaaa
     8341 ataacttatt cagtacaggt caaaagcagt tgatcagtat tgctcgaacg atcatcacaa
     8401 atccatcttt attgattttg gatgaagcaa caagtaatgt agacacggta acagaagcaa
     8461 ggatccaaaa agcaatggat gaagcaatca aaggaagaac cagctttgtg attgctcacc
     8521 ggctgaaaac aatcttgaat gcagatagaa tcgttgtctt gcgtgatggg gaagtcattg
     8581 aagaaggcag tcatcacgag ttattagcag aaaatggatt ttatgcggaa ctttatcata
     8641 atcaatttgt atttgaataa agaggttgtg aaaaagctgt cctgcatcaa gtaataagaa
     8701 cagccaatca aaaacagctt ctcatgtttt tgatttggtt ggacttatta ccgcagatat
     8761 aagcttttga gcaccgtgaa taaagaggtt atgaaatcaa cgctctaacc tgagtattag
     8821 agaaaaaaac gcaaaacata tcatttatgt tttgcgtttt ttatttatct ttttttctca
     8881 atacaatata aaataggcgg ctggttcttt tggttgataa accgataatt cagcacatta
     8941 tatgtttcct gtggcagttt ggaacaaaaa gaatcaacca tatccagttc tttttctcca
     9001 ccttcatgac catagtagac gacaaggatc aatcgtccgc gaggcacaag tcgtttcaat
     9061 atttcttcca tagctgtgcg tgtagtctct ggcatagtga tgattgcttt atcacttttt
     9121 gggagatacc ctagattgaa aatccctgct ttgagatttt gttgttcagc tatcacttct
     9181 cctaacgttt cgtggccttg tagaaacaat gagacacgtt cttctaactg acgctgttct
     9241 agcctttcac gagtagcatc gattgcttgt tgctggatat caaaactata gacatgacca
     9301 gtttttccga tgtgttctgc tagaaaaacc gtatcgtggc cattgcccat cgttgcatct
     9361 acgacgtgat cccctggttg caagatttct tgcagtaatg tatggctgaa atgcaaagca
     9421 gtttgcaaca tcagacgctc acctttttcc gtacatcata ttttccttgg aagctatctc
     9481 tgcgaagaag ttcttcatca attgcattca agacttccca ttttttcaag ctccacattg
     9541 gtccaatcaa ggaatcccac ggcgcatccc ctgtcaaacg atggatgatg atctcttttg
     9601 gtatcatttc cagctggtcg caaatgacat tgacatagtc aggacggctc attaattgaa
     9661 ggcgaccttc atgatagtcc cgcaacattc tcgttttgcg catcagatgc atcaaatgga
     9721 gcttgattcc ttggatatct gaatctaaaa tggtgcgtct gacattttct ttcatcattt
     9781 ctaaactttc gcctggtaat ccgttgatca aatgcgtaca aacacggata ttatgtttgc
     9841 gtaatttagc cacgccatct aaataagttt gataatcatg tgctcggttg atcagttttg
     9901 aagtttcttc aaaagttgtt tgcaatccaa gttctaccca taaatacatc cgctcgttca
     9961 gttcagcaag gtaatcaact acttcatctg gcagacaatc cggtcgtgta ccgactgata
    10021 atcctacgac acctggtaaa ttgactactt gctcaaatcg ttctttgatt atttcaactg
    10081 gcgcatgggt gtttgtaaaa ttttggaaat aaacgatgta ttgttggaca tgaggccatt
    10141 tttgatgcat catgtcgatt tctttctgga attggattgg aagagggtct tcaggtgcaa
    10201 cgatcatgtc tcccgaacca gatacactgc aaaaagtaca accgccatgg gcaacagtcc
    10261 catctcgatt cggacagtca aatcctccat cgatcggcac cttgaaaatt ttcccgccaa
    10321 actcatttcg taaagcgtaa ttccaagaat gatatctttt atttccttct gaataaggaa
    10381 aagtcattaa aagtccttct ttctgtaaga gttatctatt tgttttcttt tttctctata
    10441 cactggataa gtcaggcaca gccaagaaag actgagtaag taaccgccga tgatatcagt
    10501 aggaaaatga acgcccagat aaatgcggct tatcccgaca cttactataa taaacatcat
    10561 cagcaattga agcaatgtgc gtaaggttct tttttgaaca aaagctggaa gcagaaatag
    10621 taaagaaccg taaagaatca tactgcctgc tgcatgtcca ctagggaaac tgtaacttgt
    10681 ttcaactact aagtgcgcca atgaaggacg ctctcgcatg aaaaatagtt tgatcaccgg
    10741 gttgatgatt ccagcaatta caataaatcc agaaacgaac caaaatgttt ctgccctttt
    10801 tttcttatac cacatccata aagccgcaca agcaaataaa atgatgacgg taagaggatt
    10861 accgaattta gtcataaaga gttggtagct gttcagatca ggataagaag ctctgacaat
    10921 gcctgtaaac cactgatcga aaccagaaag ccacgacaga tggaacttta cgatccagcc
    10981 caaaatgaaa aacaggacaa gaaagaagct accagccacc tgaaatgatg ttttatattt
    11041 catacaattc ctcctttgaa gcaatgactt attatagcat ttttggtcca gcgaaaaaaa
    11101 cggtaaagag ataaaaccaa tgtatgattc ctctttgaat aaccaatatt cttataaaaa
    11161 aaataactgt aatcggaaag acattttttg gtaatgcaaa tgaaatgtta gggaaataaa
    11221 atcagtgttt ttcgaaatcc ttttaaacaa gaacgtagac gtaggaagaa cttgaaaatg
    11281 aacagaagtt gttcattacg gtaaattaca atatgattac aaaaaacaac aaagctctta
    11341 aataactttt aataaatatt aagagaagtt ataattatta ataatagata aattgaagtt
    11401 ggaggataga agatggaaaa cattgcaaga aaagaacgga gacgtttaga acagaccaaa
    11461 cgttttcgaa aagcaaaacg aggggcagca atcgtgggaa ctgcgatggt aggctgttct
    11521 gtcgctgccc cattagtaca gccggttcct gttcaagcgg atgaaacacc tgttcagttc
    11581 ggcgcaagaa tcaatacagc ggcatttatc acagaaattg ctacttatgc tcaaccaatc
    11641 gctcaagcca acgatctata tgcatcagtg atgatcgcac aagcagttgt agaaagcggt
    11701 tggggcagta gtgcgttatc tcaagcacct tattataatc tttttgggat aaaaggcagc
    11761 tatcaaggac aaaccgtcta catggatacg cttgaatttt taaataatca atgggtaaat
    11821 aaaaaggagc ctttccgaca atatccttct tttgctgaat ctttcaatga caatgcctat
    11881 gtacttagaa atacttcatt tggcaacggt tattactatg ccggtacttg gaagagcaac
    11941 acgaaatcct atacagatgc gacagcttgt ttgacagggc gttatgcgac tgatcctggt
    12001 tacgcaggca aattaaataa tatcatcgca acttacggat tgacccaata tgatacacca
    12061 gctagcggaa acgcaggcgg tggagcgact acaggtggca ataatactgg aagtgcaggt
    12121 agtactggcg gttcaagcaa ttctggtaat acaaactcag gttcagcaac ttcgaccact
    12181 tatactgtcc aatctggtga ttctgtttgg ggaatctcaa ataaattcgg tatcacaatg
    12241 gatcaattga tccaatggaa caacatccaa aacaatttca tttatccagg acaaaaactg
    12301 acaatcaaag gtagccagga aaatggctct tcgacgaata attccggtaa caataccaat
    12361 tcttcaggaa atgctggtac atcaaacgga ggacaaacaa ctggtgcgaa atacactgtt
    12421 caatctggtg attctgtttg gaagatctca aatgatcacg gaattacgat ggctcaattg
    12481 attgaatgga acaatatcaa gaacaatttc gtttatccag gacaacagct aatcgtatca
    12541 aatggcggtt cttctaatgc tggagcaaat actggctcaa atactgggaa tacaagtggc
    12601 tctagttcgt ccagctcaag ttcttctaat acagcttcag gaacgaaata tacagttaaa
    12661 gccggagact ctgtttggag cgtctcaaat aaatacggaa ttacgatggc tcagctaatc
    12721 caatggaaca atatccaaaa taactttatc tacccaggac aacaactgac tgtttcaaac
    12781 agtggatcaa caaacagcac gtcaggtaat acaggttcgt caaccaatac aaataacagc
    12841 agttcagtta atactggcag cagttcgtct caaggcactt ataccgtgaa agcaggtgaa
    12901 tctgtttgga gcgtggcaaa taaaaacggc atcacgatgg atcagttaat cgaatggaat
    12961 aatatcaaaa acaacttcat ttatccagga cagcaattga tcgttaaagg tggaacttcg
    13021 gcaaatacaa atacaggttc aactacttct gcagcgaaac caaatacacc gaatacgtct
    13081 gctacgacaa gtacatcatc cactggtaat acgatgtata ccgtgaaagc gggcgagtca
    13141 gtttggagcg tagcgaacaa acatcatatc acgatggatc agttaatcga atggaataat
    13201 atcaaaaata atttcatcta tccaggacag caagtgattg tgaagaaagg ttcatctcaa
    13261 tcaacaaacc aacaatcagt tactggttcg aaaacctata ctgtgaaatc tggagaatct
    13321 gtttggagtg ttgctgacag tcatggaatt acaatggctc aactaatcga atggaacaat
    13381 attaagaaca actttattta tccaggtcaa acgttgattg tagctaaata aaatagctgt
    13441 tcgaagaaat aacgattgaa ttccacaaaa atatttgtta ctataaggct acttaaatat
    13501 gacaacactt gaaaaggaag tagtaaaaag actgaagttt cagagagcgc atggctggtg
    13561 agaatgtgca cggatgcttt tgaactcgcc tttgagttgc ccttgctgaa tacccgagta
    13621 agcaagcggc ggtgacggcc gttacaacgt tcgaggcctt ttcactaagg taaaattagg
    13681 tggtaccgcg tagattacgt cctataaaaa gagcaatctt tttataggat ttttttattt
    13741 cttttgtgaa gtttgagaaa acaaagcaaa aggaaataaa aataaacaga ttctgccggt
    13801 tttttcgtta tatttataat agaaacaagt gttttcaata gaacaggagg acagcaaatg
    13861 agttacaatc ataaagaaat tgagaaaaaa tggcaaaaat actgggcaaa aacgaatact
    13921 ttcaacaccc atgatgatcc tgaaaagcca aaattttacg cactggatat gtttccctat
    13981 ccatccggtc aagggctaca tgtagggcat cctgaaggtt atacggcaac ggatatcctt
    14041 tcacgtgtga aaagaagtca aggatacaac gtgctgcatc caatggggtg ggatgcattc
    14101 ggtctgcctg ctgaacaata tgcgctagat acaggaaacg atccagctga gtttacccaa
    14161 aaaaatatcg aaacattccg acgccaaatc aattctttag gatttagtta tgactggaat
    14221 cgcgaagtga atacaactga tccggagtat tataaatgga cacaatggat ctttacgaag
    14281 ctttatgaaa aaggtctagc ttatgaagca gaagtagcgg taaactgggt acctgaatta
    14341 ggaacagtta tttcaaatga agaagtgatc gatgggaaaa gtgaacgtgg tggctatgat
    14401 gtagtcagaa aaccaatgcg ccaatggatg ctgaaaatca ctgcttatgc tgatcgctta
    14461 ttggatgatc tagaattagt tgattggcca gaaagcatta aagaaatgca aagaaattgg
    14521 atcggtcgtt cagtaggagc aaatattgaa tttaaagtgg ctggtacgga taaatcctat
    14581 accgtattta cgacacgacc agatactcta ttcggtgcaa cttactcggt tcttgcacct
    14641 gaactagatc ttgttagaga aattacgacc cctgaacaaa aagctgctgt agaagcttat
    14701 atcgaagaga cagctaaaaa atcagatcta aagagaacgg atcttgcaaa agaaaaaaca
    14761 ggagtattta ctggtgctta tgcaatcaat ccagttaatg gcaaggaaat cccaatctgg
    14821 atcgccgatt atgtattatc ttcatacggc acaggagcca tcatggctgt tccagcacat
    14881 gatgaacgtg actttgaatt cgctcaaact ttcggactag aaattcttcc agttatcgaa
    14941 ggtggagatg ttgaaaaagc tgcttacaca gaagatggta cgcacatcaa ctctgaattt
    15001 ctagacggca tgaacaaaca agaagcaatc gataaaatga atgaatggct agaagaaaat
    15061 ggcgtaggga aaaaagaagt cagctatcgc ttacgtgact ggctattctc ccgtcaacgt
    15121 tactggggtg aaccaatccc aatcatccat tgggaggacg gaacagtcac agctgtccca
    15181 gaagaagagc taccattacg gttgcctaag acaaaaaaca tcaagccaag cggtactgga
    15241 gaatctccat tagcaaatat cgaggagtgg gtcaatgtcg ttgatcctgt aacaggtaaa
    15301 aaaggccgtc gcgaaacgaa tacaatgcca caatgggcag gaagctcatg gtattacctg
    15361 cgttatatcg accctcataa caaaaaagaa ctagcagatt atgaaaagtt gaagcgttgg
    15421 ctaccagttg acatctacat tggtggagca gaacatgcgg tacttcattt actatatgca
    15481 cgattctggc ataaattctt atacgatatc ggtgttgttc ctacaaaaga accattccaa
    15541 aaactataca atcaaggaat gatcttaggc gaaaacaacg aaaaaatgtc aaaatctcgt
    15601 ggtaatgtag tcaatcctga tgatgtggtc gaaacttacg gtgcagatac actgcgcatg
    15661 tacgaaatgt tcatggggcc attagatgct tcgattgctt ggagcgaaaa tggcttagaa
    15721 ggaagccgta aattcttgga tcgtgtatgg cgtttgatcg ttgatgagaa caataaaatg
    15781 cgtgaccgta tcactacatt gaatgatggc aagcttgata aagtgtatca tcaaacagtg
    15841 aaaaaagtga cagaagacta tgaaaatctt catttcaata cagcaatctc ccaattgatg
    15901 gtctttatca atgaagccta taaagtggac gctctgcctt atgaatatat cgaaggattt
    15961 gttcaactac tcgcaccgat tgctcctcac atgggtgaag aactatgggc aatccttgga
    16021 aatgatggtg gtatttctta cgcaccatgg ccaacatacg atgaagcagc acttgttgaa
    16081 gacgaagtag aagttgtttt ccaagtaaac ggtaaagttc gtgcgaaaag caatgttccc
    16141 cgagacttag gaaaagatga gctagaaaaa gcagcattag ccaatgaaat agtgcaagaa
    16201 tatattgaag gcaaaacagt cagaaaagtt attgcggtac caaacaagtt agtcaatatc
    16261 gttgctaatt aattggctct ttgtcaataa ggactgatga gttgtgcaaa atcaaatctg
    16321 ggccagaatg aacttcattc tgtctcagat tttttgttat ttcaaattga ttaattgatt
    16381 gatcaaaaag gttttgattt tcatgttctc aaacacgttc aatgtctttt tcttctgaat
    16441 ggtcttgtaa gagatctctg taatcatcaa atttttgtct attaatctta gtattttttg
    16501 atgtataaac tacctgaaat tgagagtaca taccaaattc ataaattttt tattagtggg
    16561 actagtgatt gtatggattt tgacgcataa tgaatgaagt atgagaaaag gagagacaaa
    16621 tggatcaaga actatttatt atcagctaaa caaaaaatta tcgagaaagc gaaaaaaaga
    16681 gcgaaaagaa taatgttcaa gcttttaagc ttagtatttt tctctggttt gctttttttc
    16741 ggagctgtat cgttcctata tcgccatatg agtgcagtag tccgttcagc tgaagggatg
    16801 caaaaattaa ataatttgta tcacttttcg tggggagttg gcggaataac aattgttgtg
    16861 cttattgttt ttcttctact tatttggctc cgttaccatc aattgtcgcg tcatttcgat
    16921 caatggctta gtcaaagagc acaacagttt ttacataata aaccaacttc tcaagatgat
    16981 gactatcttt attttgagaa gacttcgacg agtcaagctt ttcggataca aaaaagtgat
    17041 tgccagttat tgctgagatc acttgatgat ttacctatct acttaggcga aagcaaatca
    17101 aaattcggaa tgaacacatt ccatattttt ttactagaaa acgaacccag atgggaaaaa
    17161 agccagtctt cgcctggtgg atacctgaca actaagatga gtacattagt cactggtttt
    17221 ttcctcattc ttttagctgg aacaatctat tatgggagat acactcagcc aaaaatgtct
    17281 tctcatataa atgctgcttc tcacatgaca atgaactcaa ctaacgatac gcatatacaa
    17341 aagacaaatg aagggttgtt agctgatcct aagcaaaagg taaatttgac aacacaaaag
    17401 ataaatgatc tcaaacttga tacagagacg cactctctct atatgacaac agatagtggt
    17461 gctaattggg tatttgtgcc gattgattcg caatggttgc gttttggcga atacaccctg
    17521 acaacggggc tgattcctga aggttattgg atggacaaca catatgatat ttccgccgat
    17581 ttctcatggt ttatctattc ttctggggat gatgtacgtc tgctgacttc aagagataat
    17641 ggtaaaacat ggcaaacgtc tattatcacc caatcagttc agccgatacg ttatcgaaaa
    17701 gcggtatttt atggcaacga tggtctattt atctgtacat cagccagtgg gatgtctgca
    17761 gaaactttgt acatttacac tactatggat ggcggaatca cttggataaa aagtggaagt
    17821 acaatgatca atcagccagt acaaaatgtc agcttcgtta caacttcttt aggttttgtt
    17881 tcgacacgtg aaagtttgtt ttatacaaac aacggaggac gctcattcaa agaatcagtt
    17941 ctgtcgattc cggaaaacta tcagacaggg gggatagatc tttttcaatc accaaatgaa
    18001 gttactcaaa taagcgcgaa tacgttagag acaaaatttt atttgttgaa aaaaggacca
    18061 atcgatgcag ggaaaatgtt tgcctgcctc ttccgttcaa ctgataacgg ggaaacatgg
    18121 caattcgttc agcaactatc gcaagtagaa caaggagatt aaagaagaga gattgtgagc
    18181 ctgacgttca gcaccgagtc acaagaaaca aattcaaaaa acagcttctc atatttgttg
    18241 aatagtgtaa ctcgtgattc ggagttgaca agagcaacaa gaagcgattt tgaagtatag
    18301 cttctcgtat tttacaagat cgtgtcttat tgccgatagg ttgtctcttg aacaccgttt
    18361 attcgtaaaa agggattgtg acaagtcttt tgtcactact taccgaacga aactgaagga
    18421 gctatccaaa aagtatcatt agtattattc tttgtgtttc tagtggcacc actagtacat
    18481 ttaaaaaagc tatcgatcat ggactaatca tacatgatcg atagcttttt agataatgtt
    18541 tgttacgcta tctgaaattg actattataa agactggcat aaagcgttgg tgattggagt
    18601 agttcagaat gtgttccttt ttcgacgata tcgccattct tcattacaag gatcatgtct
    18661 gcattttcaa tcgttgacaa acggtgtgca atcacaaaac ttgtcctatc ttttgtcaca
    18721 gcttccattg cttgctgaat caatgcttct gttcgtgtat ctacgctaga agttgcttca
    18781 tctaaaataa cgacaggagg attcgctaga atgactcgag caattgtcaa taattgttgc
    18841 tgtccttgag ataacgtgcc gttttcgcta gaaatgatcg tatcatatcc gtctggcagt
    18901 gttcgaataa agtgatcaca ttgtgcgatt tcagccgctt cgataatttg ttctcttgtg
    18961 gcatccattt tcccgtaggc gatattatca gctaccgttc cttcaaacag ccaggtattt
    19021 tgtaaaacca tcccaaaaga acgacgtagt tctccacgtg aaagcttggt aatatcaaga
    19081 ccgtcgaatt cgatctttcc tttattcagt tcataaaaac gcatcagcag attgactaaa
    19141 gtcgtttttc ctgcaccagt tggaccaacg atcgctaccg tttgttttgg ctttactgaa
    19201 aaatcgactt gattcatcaa tattttatct gatgtgtaac caaactgtac atttttgaaa
    19261 gctatttttc ctttcggttg ttctaaaagt cttgcatctg gaaggtctgg aatttcatct
    19321 gtttcatcta atatctcaaa tatcctatcg attgctgcca ttgcggattg gatcgagttg
    19381 atcacataag aagcggtaga aattggttca gaaatctgat tgatgtactg aagataagct
    19441 tgtaagaagc cgatcgtcac accacctgat agaacaagca tagcaccgaa taccgcactt
    19501 atgatgaagg cgagttgatt aatgaaacga atggccggat agatagcaaa gttcataaat
    19561 tgagcacgtt taaaagcgtt gtattgcttt ttatttactt cgtgtatagc agcttctgca
    19621 tgagcttgct gattgaatgt tttgaccact agattaccag ctaagatttc ttccattcga
    19681 ttatttaaaa cacccagttc atactgattc tgttcagaaa tctgtttgtt tttgttcgca
    19741 atcttagttg taacaattga actgccaacg atcagcaata agacgagcaa agtaagcttg
    19801 ctttcgatat aaaacagcat cagaccagca gcagctattg ttacgacaga agtaaaaaat
    19861 tgattgatac ctgtcaataa cacttgagaa agttgattga gaccggtagt tgtccgacta
    19921 atgatgtcac ctacttgatg attgtcaaaa aacgacatcg gtaatgtttt gaattttttt
    19981 gtgatttctt ttcgcaccct caatgtgatc ttttcgctta acgctgccat tgtccgttcc
    20041 tgaataaatg aagtgatgct gcttaagact gaacagccga tcagcaataa gacagggaac
    20101 aacaatgctt gtttgatatc actgaaagaa agctgtccaa tacctgtttt tcttatttct
    20161 gataagagat catcgatccc gattcccatg atcaagggca tggctacgac taataggttc
    20221 ccgaataaac tgcatagaaa caatccgtaa aaatatttat gttcaggttt gatcattttc
    20281 caaaaacgta tcaaagcacg cgtgttcaac gtttttttct tttccatcgt atcccttctt
    20341 tctatattcc ttgtgatttt gcaaactcct gataagtttg gcaattagac agtagttctt
    20401 catgagtacc ttgtccaacg atttttcctt catctaaaac aatgatgtta tctgcttgtt
    20461 ggattgtact gaggcgttga gcaacaatca cgagagtttt ctccgacatg tgtttatgaa
    20521 gggctgctct taatgctgca tctgttttat agtctaaagc ggagaaacta tcatcgaata
    20581 catagatctc tgctggtttg atcaacgctc gagcaataca tatccgctgt ttctggcccc
    20641 ctgaataatt agaaccgcct tgagcaacaa agctgtccaa tccgtcagga agggtgtcca
    20701 ctagttcttc taattgagcg atcttgatcg ctttagacaa ctcttctgtt gaggcatatt
    20761 catttcccat ctgcaagttg cttaaaattg tcccgctgaa taagaaagcc ttctgaggga
    20821 cataactgat gtgggagcgg atctgttctt gagtcatttg agtaagagga ataccaccaa
    20881 gggttacttt tcctatcgta gggtcactaa gtctcaagag aagtttcgcc aaactacttt
    20941 ttccagatcc ggtcccgcca acgatcgctg ttgtttttcc tttaggtatt gtgaaatcaa
    21001 tatcttctag aactggatca gagatttcag tatatttaaa tgtgacatgt tcaaaagcag
    21061 ctgcatttgt ctgatcaaag gttggttcag ctactgtttt gatcgtctct tcctcaattt
    21121 cgatctctgc ttgcagtatt tcttctatcc gtcgtaatga ggcaaacgat ttagggataa
    21181 tgaccaatac catagctgcg atcatcaagt aagcaagtgt cagcatagag tattcgacta
    21241 cagcagtcac gacaccgatt tgtaatgctc cttctgctac aagatttccg ccaaaccaca
    21301 aaatagaagc ataaacgatt cccatgagca aaaaagcaat tggagtgacc caagcaaata
    21361 ttcggttcac tttgatcatt tgattagcat agtcagcaaa gctcttattc gttcgctttt
    21421 cttcatatga ttgattatca aaggcacgga tcatattaat cccagtaaag aattctctca
    21481 atgtgatggt gatccgatcc atttttggct ggatactcaa agataaaggt gcccctcgtt
    21541 tcatcagtcg ataaacaatc catacataaa aaacaacaga gatcaatgta acccatgtta
    21601 gtaatagaga ataacgaaac atcatgagaa tggtgaaaac gcaaataacc ggtgctggta
    21661 agatcatttg gcaaaataaa acaatcattt gctggacatt atcgacatca ttcgtcattc
    21721 ttgtcaaaag agatccagaa ctaaaacgac tgatatcctt cattgacagc tgttggaatt
    21781 tacggaaata tttctcccgc gtttggaatc caaacctagc ggaagccaat gctgaaaagt
    21841 aactagaggc aactgcagcg attgttccta gaaacgctac aagcagcatt tcaactccaa
    21901 ttttttttat aactgaggct tcgccacttg ctatacctgt gtcgatcagc tttgctacta
    21961 attttggtac acctaatgta ccggcgattt gtaaacagat catcacaaag gtaaggagag
    22021 cgagtccttt gtttttctgt aagaatgttt ttaagataga catattattc ctcctttttt
    22081 cctgatactt ttcgattcta taccttcaag taacttgaag gtcaatagaa aaaagaataa
    22141 actgaaaaag aggtgagaaa aaatgaaaaa aatgtatacg attggccaag tatcggaact
    22201 atttgatttg ccaaaatcaa cgatccgcta ttgggatgaa caaggattaa tccgttcaag
    22261 tagacaagaa gagaatgatt atcgattgtt tgatatcgat gatattttta tgctttatga
    22321 tatcgttttt tatagaaaat tagatatacc gatcaaacag atgaagaatt tatatggaaa
    22381 aacattgacg gagttatatg agacacttga tgaaacagaa agaagaatcc ataaagaact
    22441 ggtggtgatg aaacaaaaac aaaaaaagat aagggagcga aaaaaacagt tgaaattgat
    22501 gatcgataca aacgaggaag aatttcctgt agaagagatc ccttttgact gtatgatctc
    22561 aacggagttt gaagatattg tggaaatcaa aaaatttcta cctaactatt caagctttgg
    22621 gatgatgagt atgccagctt ccagttcaca gacgatgtat ggatttttta tcgaccctag
    22681 tgaagtacat ttatttactc aagacgtgat ttgggagaaa aaagatacgg ctgtttatcg
    22741 tcgattctta ttgaaatccg agatgaacca tgcggaacgt aataatattt tagaaatcag
    22801 agaaagaatg tatgaaaaag gatggaaaac aggagaagtg atcggccagt atctattgac
    22861 gaatacggat gaaaacaata tcagaactga atattatcat gcatggatag aaatgaaaaa
    22921 ataggaaata tgtgtttaag ataactcgaa aatgatggtt gttttagtta taaagaaaaa
    22981 ttttctggtt cgattacggg tttaatgtgg gggattatta gtcgtggata gatagaattt
    23041 tagtttttta gaggaaatat gtctttaaaa atggaatggt tgcaattctt agcgatatat
    23101 acgatgtaat tgtcctctta aaaaagtatg gaaaaataat tgtattgggt gttatgtata
    23161 attcatggtg aactgagact aatgagaaga aaaattcata ttgttatgag tgagattgta
    23221 tcagatgaga atgataaatt aaataggtgc ataaaagcat tacacttaaa atacattgtt
    23281 gcttacatta ttacaatatg ttaataaggc aaaaataaat agatggattg aataaacata
    23341 ttttttaatc aaatagtccc tactgtgact ttcctcagct atcggtgata ctaccgatag
    23401 catttttttg cttataaaat aattataagt atttagagat ttttcttcta acattcttca
    23461 aacaagaaaa atcacaaagg ttattgttat ttttatatat tctttttaag aatgattatt
    23521 tttttataga aattttaaaa aataaaacac gttattgatg gatagttagt ttgttatttg
    23581 gtaatattat agaagttgac tggtagtgat ttatttttcg ttgttttata aaaatgttat
    23641 tttttggaaa aattcacaaa tatgtaacga atgtttttct ggaatttgct gtgttaaaaa
    23701 gcagtatgac taatataaat actattgtca tttatgcaat tttttatgga tagtaatttt
    23761 agaggggaat caatgaagga agaatagaag ggaaaaatag ttgcaaatac gccagttagc
    23821 aaaaatagca atacatcttc aagctttaaa attcgagaga atcatcttgg tagtggaatc
    23881 aactacaaca aaaggattaa accaattaat agatagaatt accaatggaa aaatagaaaa
    23941 ggtagttatt ctatacaaaa gttgtttaat tcgcttcggt tatgaactta gtgaaaccct
    24001 ttgcaatata tggtacagaa tatagaaatt attgacatta cagaaaatga tgaagattta
    24061 atacagattg tcactatttg tagatacctt ttacaaggaa accgagccaa taaagctaag
    24121 aagatgatta aggagttgat ggaagacgat acaagttaag aaagtaaaac tacgcccaaa
    24181 taaagagcaa aaagagtaat tggacgtgga aaatgcaaga aatgaaccat cgattcggtg
    24241 gacagtttat tagtaatgac ggtttgagaa catccatgac gagaatgaag gaacatccta
    24301 aatgcgttta gttgaaccaa gtgccaaata atatggccaa ataagcgatc agttatcgat
    24361 gaatcccaag tattctaaac tatatattca ttttgatgct aagcattgat atattttttt
    24421 gggattgatg aaggttaacc aatacaggga ctcactgatg aaattctaga gtctgacctt
    24481 ggtatcaacg atttagtgat tgtttctaat ggaaaagtat aaaagtatat aagtacatta
    24541 acaaaataac tattagggtt acgctccagc ttgtgcaagg cactggcagg aggaatcgtg
    24601 aaatcataat tatggttaag ccacacggac aactaaaagt catacaaagc tatcgaatca
    24661 atcaacagaa gacaatcggc aatcattagc tttgtttaca gcttacattg caacgcttga
    24721 gttgaaccga aaatacgagg gaggtaacgc agccattttg agttggggaa tctttttaga
    24781 aaattatcgg gtaccttgtt gtttagtcaa aattataagg gggagtaata gtgaggaaca
    24841 aatttcataa actgttaagt atgtttctag ttatgcttat tgtgatcaat caatttggga
    24901 acataacggt tctagctgag tctttagcag aacagccaga gcgttcgctg acgatcgaag
    24961 ttgatcaaga ggaggctgct ttgggtgagc agttccagtt gcgagtcaga ggaacaccag
    25021 acctgctaaa tgcattggtc attgataaga gtgaggattt gattgaaggg gaagtgaagc
    25081 aactcaatga ttacgaacgt ctgattactt attcttccca gcattcaggt aaatacgaat
    25141 tgaccgcaag aagcggtgat attcaatcaa atacggtctc aatagaaatc agtgtaccag
    25201 aagaaagcac aagtgagtct tttaatgtac cagaagaaag cacaagtgaa tcttttaata
    25261 taccagaaga aagcttaagt gaatctttta atgaagccag atcatctgag ccatctataa
    25321 cgcaggaatc tcagattgaa agacaagagg agctcttgaa ttatgacatc ggcatctctg
    25381 ctgagcgccc cactttgccg agcaggcata cacaaacgtt aaagggatac attcaaggag
    25441 gcgctggcga tattcttgaa gaatatgggg agatggtcgt acgagttcct agagcatcga
    25501 ttggcttaat tgagttcatt agagtggagg tcgctgatga ttcaccttta aagccactgg
    25561 cattttacca agaaggcaca gacgttgttt ttccatttac ctttgattat gaaggattaa
    25621 atgaagccgc tcagtcgtat acattcagta tcacgtttga agttgctttc tttcaatcag
    25681 gcggtactga gccaaataat gactttcaag tgagtcttac ttatgctgag cgagcagcaa
    25741 acacggaatt ttcaattacg cctattcctt catcaagacc cattttttcg aaattatact
    25801 atggctctag agaaatcgac agccaaggaa atcttgggaa tctgctgatt gacgggcaag
    25861 acgtgaccaa cagtaataaa tttattttgc aggtaaatta tgaaggatca cgaaaagacg
    25921 gtgtagtggt taccgatgta ctaccgcaag gaacaacttt ggcaccgaat ccgactacat
    25981 ggagttccac tggaaataga gaacctattt caggtggggt tcggatcgca gaagtaacga
    26041 atccaggtga agcaaacgtt agaactcatt atgtttccga ggaatttgtg gacaaagtat
    26101 cttttaataa ggaaacgaat acacttgttg tggactttgg taatctagca gagggtcaat
    26161 cgtattttat tgaatatgct cttgaagtga ccgatgtcga cttgttcaat gaaggaagac
    26221 aagaggatgg gttttctcgg aatcgagcga gtatgaccta caataatcag gtaatgagcc
    26281 gagactaccg cttgagaatc cctgaaagta atcttccggc aatctcttta agaaaaagtg
    26341 tggacaaaaa tgttatcaat ctcaatgaaa atgaactaac gtatactctg catgtctcga
    26401 tgttttctgg aacaagcaat acgcttgtta ttgaagatgt tttggaagaa ggcatggaat
    26461 ttgtgtcatt tctatcagat attccctctt tcattgatgc cgaagtcaat aatggaatag
    26521 ttcgtttcag tgcgaataat acgattggtg gtccgacaag ttttaccctc tcttttaagg
    26581 tcaatgtaga ggcgtatgcg gtcggcgatc aaattttaaa ctttgcaacg cttgtgaata
    26641 gtgacaatcg attgaatacg agtactgtaa gaacaaggaa aatcgatggg cgtattaaag
    26701 taattaaaac tgatgagaat ggccaaaggc tggcaggagc aaaatttgaa attttatcaa
    26761 gcgattcaca agtgatacaa gaaggtataa cggcagaaaa cggagaattt ttaagtgaac
    26821 cgcttcctgt aggtagctat caagtccgag aaacagaagc accggaaggg tatcttttag
    26881 atgcgacggt acatgatgtt tttataaatg aatactccat agaagaagtg acgttaacca
    26941 ttgaaaacat ccttgatacg ggtggtgttg aactaattaa acatgctgaa ggcagtggtg
    27001 aagtccttca aggagctgtt tttgagttac agaaccgaga aggagaaacg ctacaaaccg
    27061 gtttaactac aggagaagat ggaaaactgg caattgacgg tttagcacca ggagcatatc
    27121 agctggtaga aactcaagcg ccaatagggt atgaattaga tgcgacacca atcgagtttg
    27181 agatcgaaag aagtcagaca gcggtggtag aattgacgaa agaaaaccga cttacaccag
    27241 gcggtgtcgt gttgacaaag atcgatgacc aaagcgggga aatcctgcaa ggagctgttt
    27301 ttgagttaca gaaccgagaa ggagaaacgc tacaaaccgg tctaactaca ggagaagatg
    27361 gaaaactggc aattgacggt ttagcaccag gaacatatca gctggtagaa acccaagcgc
    27421 caatagggta tgaattagat gcgacaccga tcgagtttga gatcgaaaga agtcagacag
    27481 cggtggtaga attgacgaaa gaaaaccgac ttacaccagg cggtgtcgtg ttgacaaaga
    27541 tcgatgacca aagcggggaa atcctgcaag gagctgtttt tgagttacag aaccgagaag
    27601 gagaaacgct acaaaccggt ctaactacag gagaagatgg aaaactggca attgacggtt
    27661 tagcaccagg agcatatcag ctggtagaaa ctcaagcgcc aatagggtat gaattagatg
    27721 cgacaccaat cgagtttgag atcgaaagaa gtcagacagc ggtggtagaa ttaacgaaag
    27781 aaaaccgact tacaccaggc ggtgtcgtgt tgacaaagat cgatgaccaa agcggggaaa
    27841 tcctgcagga gctgtttttg agttacagaa ccgagaagga gaaacgctac aaaccggtct
    27901 aactacagga gaagatggaa aactggcaat tgacggttta gcaccaggag catatcagct
    27961 ggtagaaact caagcgccaa cggggtatga attagatgcg acaccgatcg agtttgagat
    28021 cgaaagaagt cagacagcgg tggtagaatt gacgaaagca aaccgactta caccaggcgg
    28081 tgtcgtgttg acaaagatcg atgaccaaag cgg
//
LOCUS       DACDUU010000034        27402 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.034, whole genome shotgun sequence.
ACCESSION   DACDUU010000034 DACDUU010000000
VERSION     DACDUU010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.034"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            281..1759
                     /locus_tag="IWL30_001478"
     CDS             281..1759
                     /locus_tag="IWL30_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9706245.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            2036..4096
                     /locus_tag="IWL30_001479"
     CDS             2036..4096
                     /locus_tag="IWL30_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706246.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            4179..4748
                     /locus_tag="IWL30_001480"
     CDS             4179..4748
                     /locus_tag="IWL30_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9706247.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(4923..5729)
                     /locus_tag="IWL30_001481"
     CDS             complement(4923..5729)
                     /locus_tag="IWL30_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706248.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            5867..7381
                     /locus_tag="IWL30_001482"
     CDS             5867..7381
                     /locus_tag="IWL30_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAP9706249.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(7784..8767)
                     /locus_tag="IWL30_001483"
     CDS             complement(7784..8767)
                     /locus_tag="IWL30_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAP9706250.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(8772..9419)
                     /locus_tag="IWL30_001484"
     CDS             complement(8772..9419)
                     /locus_tag="IWL30_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9706251.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(9504..10136)
                     /locus_tag="IWL30_001485"
     CDS             complement(9504..10136)
                     /locus_tag="IWL30_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9706252.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(10152..10703)
                     /gene="coaC"
                     /locus_tag="IWL30_001486"
     CDS             complement(10152..10703)
                     /gene="coaC"
                     /locus_tag="IWL30_001486"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAP9706253.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(10700..11437)
                     /locus_tag="IWL30_001487"
     CDS             complement(10700..11437)
                     /locus_tag="IWL30_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAP9706254.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(11434..12423)
                     /locus_tag="IWL30_001488"
     CDS             complement(11434..12423)
                     /locus_tag="IWL30_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP9706255.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            12681..13223
                     /locus_tag="IWL30_001489"
     CDS             12681..13223
                     /locus_tag="IWL30_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9706256.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            13240..14325
                     /locus_tag="IWL30_001490"
     CDS             13240..14325
                     /locus_tag="IWL30_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9706257.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            14337..15143
                     /locus_tag="IWL30_001491"
     CDS             14337..15143
                     /locus_tag="IWL30_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9706258.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            15144..15971
                     /locus_tag="IWL30_001492"
     CDS             15144..15971
                     /locus_tag="IWL30_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9706259.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            15968..17041
                     /locus_tag="IWL30_001493"
     CDS             15968..17041
                     /locus_tag="IWL30_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9706260.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            17469..20165
                     /locus_tag="IWL30_001494"
     CDS             17469..20165
                     /locus_tag="IWL30_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9706261.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            20242..20730
                     /locus_tag="IWL30_001495"
     CDS             20242..20730
                     /locus_tag="IWL30_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9706262.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            20742..21236
                     /locus_tag="IWL30_001496"
     CDS             20742..21236
                     /locus_tag="IWL30_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9706263.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            21257..22066
                     /locus_tag="IWL30_001497"
     CDS             21257..22066
                     /locus_tag="IWL30_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9706264.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            22063..22884
                     /locus_tag="IWL30_001498"
     CDS             22063..22884
                     /locus_tag="IWL30_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9706265.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            22935..25034
                     /locus_tag="IWL30_001499"
     CDS             22935..25034
                     /locus_tag="IWL30_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP9706266.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            25031..26149
                     /locus_tag="IWL30_001500"
     CDS             25031..26149
                     /locus_tag="IWL30_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP9706267.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            26188..27066
                     /locus_tag="IWL30_001501"
     CDS             26188..27066
                     /locus_tag="IWL30_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9706268.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            27257..>27402
                     /locus_tag="IWL30_001502"
     CDS             27257..>27402
                     /locus_tag="IWL30_001502"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706269.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEF"
BASE COUNT         8755 a         4707 c         5733 g         8207 t
ORIGIN      
        1 aataagtaaa agagcaggac ggtggccgtc ctgctctttt ttgtggttgt ttacgcaaat
       61 ggcgcaaatc ttgtacattt taacacaata aaacaaaaaa tatagaatat ttcctcataa
      121 atttttccat tttttgcaat attctcaaaa aaaagataaa aaatgagaat aactgatgaa
      181 aaaacaagca taatccatca taaaatcagt ataggtgaaa agatgaactt tcatctgcat
      241 atcaagtaat tggtatgagg cattttttga ggtgtaggat atgaagttgg attaccttat
      301 agaagaggaa gatagagaaa aactgctgct agctagatat ttagaatttg agaaaaagaa
      361 ttatgtagtg ctgcctaagt tatatgaaac attaggtttg tctaaattta aaacaagaaa
      421 ttatatcata gaattaaata aagaatttac tgaattcaaa acaaagccag agatccaaat
      481 ttcagaagaa gcagagatcc atgtcgaacg tctgaatttg acggatatca aaaatttaag
      541 aagaatctac tttcaacgat caaaaattgc tcgactgtta acggagatcg tggattcaaa
      601 agtaacgatc gaaaaatttg cagagaatca ttttttaagc atatcaagag cctatgtaaa
      661 aagaagagaa ttagtctctt ttcttgaaca gcatcaaata aagttaaaga aaaacatatt
      721 agttggggaa gaaataaaca tacggaacgt attatttggg gtatatttcg gggtctttaa
      781 tggtttttct atgccattta gagaaagcgt catgaatgaa gcaaagagta tcgtgcaata
      841 ttttgaatat ctgttcaatt taaaactgtc tgcaacaaaa cgaatcaaat tcaatttgct
      901 aaccactatt agtttgttga gggcctatgg gaatcaacca atcggaaaac gtttcttttc
      961 agatgaatac tttaagagct cagaaggaat gaagatcatc aaggatttat cacatatttc
     1021 ttttcttcat atccctaaag agcagatcat tgatgagatt tcttatatca tgttgttttt
     1081 gatcacggaa gaagaatttg aattagatgg cgcatttgga cttgcggaag ctcattttca
     1141 tgatatccac atgattagtc aaaaaatgtc agtggaattg attgatcaat taccattttc
     1201 gattgaaatc tctgaagaag aaagactata tatacgaaaa gaacttcgta tgggtatgat
     1261 gcatgtcaat cgaaaaaatt ggatattcga ctttgagatg actagcttta cgtctaaaaa
     1321 gcagctacag tttttcttcg agacgtatcc aatattcagt acagcaatcc aaaaggtcat
     1381 acaagagaat aaacgctatt tcttgggaaa agatgaacgt ttcatgattc gcttatttta
     1441 tgattatctc tttttattgg tcggtatttg tccggtatca tattttgaag caccgattca
     1501 tgtctgtatt gatttttcgc aaggatctgc ttataccgat tacattattt ctcaagtgaa
     1561 aggatttaaa aattacaata ttgtgattga gagtcgaatc acttctcgta cgaatattta
     1621 tttatctgat tgtttacttg aaaaatttaa taaagaacag ataatttgga aaaatccacc
     1681 gactccagat gactgggaat attttggaaa taccatcatc agagaaaaaa atagacattt
     1741 aactgaaaaa atcagctagt atttatggat ggcagctttg tctcacatat ttattagctg
     1801 atttatgttt ttttcaaaga aaaggtaact agatataact agatataact agatatatta
     1861 cttaacaaaa ttaaaaagta cataagttat tattccgatt ttcttgaaac aataaaaaat
     1921 caaaaaaaat actctcctaa tagtccatct tcttttgatt ttatttatat aatgaaatca
     1981 tcttatgaaa attactactt catgtttaga caagagtagg gaggactaag aataaatgtg
     2041 ttcaaaaaga cgttcaaaac aactaaataa acacgcttgt cgtttagata aggaacggtc
     2101 gtgttctgaa aaacactacg ttaagtttat gatgcgcacg atgctgatca gtttatctgt
     2161 aggtgtctat atgcatgtac ccgttcaggc aacgacacaa accatcaagg tcgtacgtac
     2221 ctctgtttct gagaccactg attcccctat cactattact catactttca ctgatgcaaa
     2281 tggaaaacag ctacttcctg cattgacttc ctataataaa gtcagttatc ctccaacgat
     2341 aaaccactac tggttaattc ctaacaaaac attatggaca tcggaaaccg gaagcagtgt
     2401 gccgctttct atcttttccg atatgactga tcctacaaaa ggtgatcagt taaaacagat
     2461 cactgatatt ttgaatcatg ctttgacaga tataaaggac ggtggaagcg cacaagtttc
     2521 ttatgtatat gaaaaagatt attcacagtt tgaaagaaag aaaatcgatg ttctggcagg
     2581 gacaaaagtt tcgatatctg atctggtaga aaggctgaag aacgcagatg gaacacaagg
     2641 agatatgtcc aaagtaattt caaacaatgg gaatatcgtg gatacgacta atacaggaac
     2701 atctactatt tccttgtcct attttgatcc aattgcttta aaaacaatga ctgcatctgc
     2761 tgtgatccat gttcgtgctg atcaaacagc tatcaaagga aaagatcttc acttaagaac
     2821 aggagagagt tacgccccta aagatcttgt tgaatatgta acaaaaagcg atggaacttc
     2881 aggaaataaa gaagaaatca aaatgaacgg gacagtagat actacaactc cgggagaata
     2941 tcccgttgta ttaacttata tagactcgaa aacatcaaat gtggcagaaa caacagctgt
     3001 agtttatgta gaagcatctg ctgcttcatc gactgtcatc actcatttta tagatagtca
     3061 aacaaataaa gaacttcaag cagaaacaca tatgagtagt gtaaatcgta ttcctgattt
     3121 gcgcctacct gctgaatttt ctgatcgagt gccaaatata gatttatcga cttatgaaca
     3181 aaatgggaaa gtcacttcta tttctgattg gatgcaagca aacggattag acagaaaaag
     3241 tcactggtcg gagatccttg aagtacttca ctcagaactg atgaataata ggaagcaatc
     3301 aggaggggaa attaccttta cttatgttta tataccagac aatcgttcat tagaaggtca
     3361 tgatcttatt gttcatccaa acgaaaaaat aaacaaagaa gagttgatcc aatcagcaac
     3421 aaattcctta ggtaaacccg taccgttaaa ggatgtatcg gttacaatca atgaagtacc
     3481 attggaggat ggttatttga ccaaacaaac aggtgatttt acgattatgt atagttatca
     3541 tgacccgtat tcactccaag aactccatgc tcagtcacta ttacatgttt taccattaaa
     3601 caaacagata gatcaaaaag aacaaaaaag acaagaagaa agcttgtcag aaaaaaaaca
     3661 acaaatggcc catgcagttt ctccaacagg gaaagtaaaa caaacaagaa gcctagagtt
     3721 agcaagaata tctagaaaaa tctcagaatc taataaaacg ataaggcaag aagctcattt
     3781 ggccccacaa gtttctggat tggaaaatga aattttagag cctaaaataa aacaacaatc
     3841 agaaaaagaa tttgttgcta aacagtcgga agatcagaag gaatcagatg accagaagga
     3901 aacgaagaaa aaacgcttgt caactattga ttccaccgat gagcatctgc agccgcaagt
     3961 accacttcct agtccgggaa cacccgcacc tggtggaggt attccttctt ctgctttagg
     4021 agatttcatg cgaggtatgg caggaacatg ggtatacgct aatcgaaatg aatttgacgc
     4081 agatgactgg ttctgattta tatctctatc tgttatagtc aaataagtag ctgagtcata
     4141 tgttatttgg cataatggag acgaggaggg atagaaaaat gttagataaa acgttacctt
     4201 acgcagaaat ctggatgaca agaccacttc aacaaaatgt tccaatcacg cctttagcgg
     4261 aaggttttga atttcgattt tatcaaaaag aagatgaatc tgcttgggcg gagattgaga
     4321 cttccgttgg ggaatttcaa acgacagatg aagcacttga gtattttcaa caaaactttg
     4381 ctccttatcc tgaagaactt gaaagaagga tgttttttgt agagacaaaa aaaggagaaa
     4441 aagtagcaac ctgtaccgct tggcgaaaaa aatcaagaga gcaagtgtat ccggtttttc
     4501 attggttagc agtgaaacca gattaccaag gaaaagggct cgcaaaagca ttgactgcca
     4561 gagtcttgca agaatttcct accctccata cggacggacc aatctatctt cacacacaga
     4621 catggagcca tcaggcgatt gctctttata aaaaaatggg ttttactttt attgctgaaa
     4681 atctagacgg cacacctaat ccagattatg aaaaagttat gcaggtttta agtaaattag
     4741 ataaataacg aaatggtgaa ataagtcctc agtattggga taaaaaacga aatagtcatt
     4801 cctcatatct tacttttttg agttaatact atcggataac taatggcatg ccatacattc
     4861 aaaaaagaag gggcagtgac aaaagtattg tcactgcccc ttctcttgtt ttttagcatg
     4921 ttttatcgtt tgattttttt aggaataaaa ttcttgatga acgtaaattt ttggtaatcg
     4981 tttcgactat agaggtaacc aaatacactg aaaacaactg ctccaataaa attaacaaat
     5041 aaatccttca tagtatccat gattcccacg tctaagtaac cattttggat gattgtttta
     5101 tctgttttac catctgcatt ttgactttca atgattgtcc gagtgatatc tggaacatga
     5161 tagacagtgt ttccgtcttt acttaactcc acactattga tttgctggat gatcgtatct
     5221 ttttgcatat cgaattgcag taatttatct gctgaaaatt ccacaaactc ccacataaca
     5281 ccaactgtca tcgaaaaaca gaaggaaaca actgctagta ataaaggagt caagttcatg
     5341 gtttttgtat ttttattgag caaataaaca agggaaaagc cgacaccccc agctaaaaat
     5401 ccgctgaatg catgtagaaa agtatcccac attgaaaaat agccataaaa atcacttaac
     5461 tctcctaaaa ctgttgaact aaaaataaaa gataacaaaa tgatttctag catatttgga
     5521 atctgtatct ttaatatctt ttccagaaaa taagggattt caaataaaat taatgtcagt
     5581 aataaaagca ttgtatttcc ccagtgttgg ttccagacct gatccactag cataataacg
     5641 acgatgatac gtaataaaat atagattccg tttgtgattc tacctgcagt ttttgtactg
     5701 ttcgtaaaaa aggttttcaa atgattcata aaataactcc ttcatgtata tccttaatta
     5761 tactaagaag cgtgtgttta ccaaagctat atgacttaaa gaaaaacaga gttgatattg
     5821 tgaaaaaaat cacttaatat agtataagga gaaaaggggg atacaaatga aaaaattagt
     5881 tgctggttta ttaacagccg ttgcacttct tgctatgagc gcatgcggcg cagatcagaa
     5941 aaatacagcg cagacaagtt ctagtacgaa ggaatctgcc gaagtatcat caggcgcttc
     6001 tgaacaagta tatacagatc caaaagaaat gaaagaatct tatgatgtcg tgattattgg
     6061 agctggtggt gcagggatgg cagcagctat cgaagcaaaa gatgctgggt tgaatccagt
     6121 aattttggaa aagatgcctg ttgccggagg aaacacatct aaatcatcaa gcggtatgaa
     6181 tgcttcagaa acaaaatttc aaaaagaaga aggaatcaaa gactcgaatg atgccttcta
     6241 tgaagaaacg ctaaaaggtg gtggaggaac gaatgataaa gaaatgcttc gatactttgt
     6301 cgatcactct gctgatgcgg tcgattggct agatcaaaac ggtatcaaac tggacaactt
     6361 aacgatcact ggagggatga gtgaaaagag gacacatcgt ccgtctgatg gctcggctat
     6421 tggtggatac ttagtggagg ggttattgaa taacgtccat gagcgggaaa tccctatttt
     6481 tgtcaacgca gatgtgacag acattaagaa aaatggggaa acggtcaatg aagtgactgt
     6541 acatgttcaa ggagaagaac caaaagaggt caccggaaaa gcaatcgtag ttaccacagg
     6601 cggttttggt gccagcaaag aatttattga aaaatatcgt cccgatttag ctgattatat
     6661 tacgacaaat caagaaggat caactggaga tggtatcacc atgattgaaa aattaggtgg
     6721 acaaaccgtt gatatggata aaatacaaat tcatccaact gtgcagcagg atgagggggt
     6781 cttgattggt gaggttgttc gcggagaagg agcaatcttg gtcgatcaag aggggaaaag
     6841 atttgtcaat gaaatggata caagggataa agtatctgca gcaattacag cattgcctga
     6901 aaaatcagct tatttgattt ttgaccaagg cgtgcgtgat cgtgccacag ctattgaatt
     6961 ttatgaccaa aaaggatatg tcacaactgg aaagacaatg gaagaattag cagaaaaaat
     7021 cgatttacca aaagaaacac tagcaaaaac agttgatgga tggaatacgg atgttgcgga
     7081 gaaaaaagat gctcaattca accgtgccac agccatggaa catgacttat cgtctccgaa
     7141 ttattatgcg atcaaaatcg caccaggaat ccattacacg atgggcggtg tgaaaatcaa
     7201 tacgaacact gaagtattag ataaagaaaa ccagccaatt agggggttat atgctgctgg
     7261 tgaagtcaca ggtggcttac atggaaacaa ccggatcggt ggaaattcag ttgcggagat
     7321 cattattttt gggcgacaag cgggcataca agcagctcga tttgcttcgg aaaatgaatg
     7381 aaatagaacg taaataaaaa gaggttgtga aatcagcgtt ttgagctaat accgagggtc
     7441 agctgattga acactgttta gtgaagaggt tgtgaaaaag ctgccctgca tcaagtaata
     7501 agaacagcca agtaaaaaca gcttcttatg tttttaactt ggttggactt attaccgcag
     7561 atgcaagctt ttgaacaccg tttattcgga aaaagagcgt gggacaaaag taaagaacac
     7621 ttttgtctca cgctctaaac acgtataaac ggcggaagca gaagaaattc cttcggaaat
     7681 aagctgaaat tcacaaaaat ttgaaaagca attttcgtga atttcttctt atttcttgga
     7741 gttaaacgct tctgtcccag cctctttttt gatcagcatg attttattca gttatcttca
     7801 aaatgatttt tccgatgttt ttgtttgctt ccatatattc atgcgcttgt ttgactgcgc
     7861 tgaattggaa tgtacggtca atgattggcc gaatcttgcc attttctaga aaaggcccag
     7921 ctttttgcat aaaagtttgt gcgagctctg ctttatactg atcacttcta ggtgtcaata
     7981 atgtaccaag cagttggaca cgtttcccaa tcaatacacc aaggtctatc tcaggcacga
     8041 tggtcccacc tagcatacca atcaagaccc aacgtccatc aacggctata gaagctaaat
     8101 tcttttccca gtaagaagcg ccgatgaaat ccaaaataac atcgactccc cgaccttcag
     8161 tgatttttcg tacctcttct tgaaagtctt gttttttata gttgatcaat tcatctgcac
     8221 caagttcttt acaaaaggcg agtttttctt ctgatcctgc agtaacgatg attttcgcgt
     8281 gagaaagctg tctagctaat tggatcgctg ctgttcccac accacttgct cctgcatgga
     8341 tcaagacagt ttcgccttct tgtaattttc caagccagta aagtgtttga taagcagtca
     8401 aaaaaacttc tgagattcca gcagcagaaa catagtctag cgtatcaggg agaaggattg
     8461 cacgatcggc aggcatcaca gcatattcgg cataaccacc tagattgact aatccgtata
     8521 cttttgtccc aggagtaaaa gtggagatat cacttttatt ctcaactacc tctccagcta
     8581 cttcgactcc caaaataggg tagggaggtt tcaaagagac tgattctctt ttcataatat
     8641 ctgttcgatt gacagctgct gtatggactt tgatcagtaa ttcgccttgt tttggtttag
     8701 gaacaggcac ttcaatttgt tctaatacat ctacagaacc aggatgcttg attgaaatgg
     8761 cacgcatgga tctattcctc ctttgcccat tcttggaatg ctgtattcaa ctctttgcta
     8821 ccttcagaaa gtactagagt cgtacgagaa atatccgttt cttccactag agcggcaagt
     8881 acagcagcaa ctgtagagcg agtgatcgtt tttgatgcct tctcattagg cttagttaat
     8941 tgaatggaag taacttcttc atcgttagtc aatgtgactg gttgaagaat gacaaaatct
     9001 aagttagttc gtttcagcca ttcatctgca taatgtttag taatataata atctgtcatg
     9061 gactcaggcc attttgctgg ttcatctgca taaacagcac tgaccatcag atagcgagag
     9121 acatgcgcat tttcagctgc gatcactgtt ttgattgcgc catccaagtc tacttgcaga
     9181 agattctttc cttgagagcc ggcagcaaaa atcaatacat cgatgccttt gaatgcttcg
     9241 gtcatttttt ccacggacca agttaaatca aaagagacat acgaaacatt ctcttttttt
     9301 actaatgttt gggaagcaac atctctcacc cctgcaaaaa tttcatggga acttggagct
     9361 aaatctttta tcaggtgtcg gccaatttgt ccgtttgcac ctacaacaaa aattttcatt
     9421 tggatcatcc tttcgacgtc aaatctttcc attcaatagt aacaccaaaa gagaaaaaaa
     9481 tcagagatcg gctacgatga ctatcagtta gtaagagaag ggtgcttgaa gtatcttaac
     9541 aagacattgc atactgctgc agtcgcaaga cctgcgacga ttccttcagg tataccactt
     9601 gtaaaaataa ccgctagaat agctgaataa acagcattta aatttgttcc gatgactttg
     9661 gcataatctt gctggaataa aaagtagatc atattcatca ctaaaatcgt attggtcaat
     9721 gaaccggaaa gtccagctaa gaataaagaa acggaatctg ctttttgttt cattagtttg
     9781 cgcagacctt tataaacgaa ataaggaacc accccaatca agatgcgagg aataaacgcg
     9841 atcagcaagg ctttccagct tccatgttct gttccgatga ctgggataaa cggagaaaag
     9901 acaaaagaca aaggtgtttg gataactgtg ctacggatca tgctgatcag tccaaatgta
     9961 ccgccaagaa aagcacctag tcttggtcct aaaacgatcg aagcaatgat tacaggaata
    10021 tgcatggttg ttgcattgat tggaccgata gggataaaac ctaaaggcgt aacagctaat
    10081 aaaatcaaaa tagctaaaaa catcgcagtt aatgtaaagt tttttgtatt tttcataagt
    10141 actccttttt tctaagcatt tctttcatta agagtcagca tgacctgttc gatgatcgtc
    10201 tgaatatcag ctaaggcacc ttttccaaaa tctccacaag ctaaaagaga ttctcttggt
    10261 tcaatttcct gatagccgac agatctcaat gtttccagat tacgctgagt gatcgggttt
    10321 tgatacatat tcgtgttcat ggcaggggca atcaattttg gtgttcccac tggaagcgcc
    10381 attgcgactg ttgaaagaag atcgtctgca atgccgtttg ctagcttgcc gataatgttt
    10441 gctgtagcag gcgcgatgag aaacaagtca gcttgttttg ctagttcgat atgattgatt
    10501 acctgcggat ttgattcttg catgacgtct gtatggactg cgcgcttcga cagagattgc
    10561 aaggttaatg gcgtgataaa atgtgtgctg cttttagaca taatcacatc tacctgggca
    10621 cctaatttga cgaattgact tgtaagatca gcgcttttgt aagcagaaat acttccagta
    10681 acgcctagta agatcttttt cattttgatt cctccagtct aattttctca gtaatcaatg
    10741 cagcaatctc agatttactt tgagcttctt ccactgttcc atctttagat aagagatagc
    10801 cgtggtgtgt ctcgccatgg atttccgtca aatcatttgc gaaaataaaa tcagttttgt
    10861 tatttatcag actatttttt gcgactgcca gaagagattc ctttggtaca tctactaata
    10921 gtttgaaacc aatgattttt gcttttggct gccattcacg tagataagaa atgatttttg
    10981 gtgttttctt caaaacgagc acaagatgct cggtgttaga agagatcttt gaaacagtgg
    11041 tatctgtctg ttcattttcc gcaaaccaat tgtccagttc ttcttgcgtt gaagaagtgg
    11101 gtaggttttt tgccagctgt tcttctgaaa aactaaaagc aggagtaaaa tcactgactg
    11161 ccatactgtg gataatcgca tcgtagtgtt cttttttcgt tagttcttct aattgtaaaa
    11221 ataagtccag tgcagattcg atcgtataaa tcgttatatt ggagtgtctt ttcggtttca
    11281 gtgcttgttt cgtagtcacg taatcgattg tggtatccgg attggcagct aaataatcag
    11341 ctaatgcttg ccctaatcgt cctgtcgaat gatttgtaat agcacgtact tgatcgattt
    11401 tttcagtcgt tcctccagct gtgattagaa tcttcattta tggtcaccta catttactag
    11461 tgttcttcct tggtgtgttc cagcaagtag tttagatgct gtttcaggca attgatccag
    11521 agtaatctct tggacgacca gttcatctgc aatctgcaaa tccgccatct gttgccataa
    11581 agaaagacgt tgatctattg gtacattgac agaatcaatt ccaatcaact ggatgtttcg
    11641 cagaataaaa ggcaagacag tcgcgtttag tttgattccc ccagcattgc cacataaagc
    11701 tactgcaccg ttataagaga tcaatggaag aagactgctt agctgctcgc cacctactgt
    11761 gtcgatgaca tagtcgattt gctgttttcc taaagctttt gttgtctctg gcagaaattc
    11821 atcaggtcgt acgacttgca aagcacctag actttttagc catgtgacag actcttcttt
    11881 tctggaaaag gcaatgattg actggaatcc tagtttatgt aacagggcta tcgctgtact
    11941 gcctacaccg ccagaagcac cagtaacgac aactttttta tcggaggtca taccttgacg
    12001 gattaaagcg ttcacacaca acatagcagt aaaccctgct gtaccaagga tcatcgcttg
    12061 acgcagcgtc atattttttg gtagaggaac taaccagtct cctggtactt tttgatattg
    12121 gctataaccg cctggatggg tgacgccaag cccaaaacca gtagcaagta caagatcttc
    12181 ttttgaaaaa cgtggattgt tactttggac gacagttccg gataaatcaa tccctggagt
    12241 catcggatat gttcgtatca cctgaccaga ctcgctcatt gccaatgcat ctttataatt
    12301 gacatcagaa taggcaattt ttaccaagac ttcattttca gccaactcag taaatggcag
    12361 atcactgatt gctgtatgga aaacaggttc ctttattacc tgaaaagatc gaaaagcgtt
    12421 catttgtgag acactccttt taaaattttc ttttatcata ccataaagcc tactctttta
    12481 ttcttgttct tccgtaaatt tagacaaaaa ttaggtgaaa aataaagaat caaacaacta
    12541 gaatttattc caaaagataa agaaatattc agcaaaaaaa attttacaaa tggttgcctt
    12601 tttcaataaa tttcgtatac taatacgagt ggtagtaaac aggaagttta ctattagaaa
    12661 acttcgattc ggaggaaatt atggaaattg gtgagaagtt aagaaatttg cgcatccaaa
    12721 agaatttgac acaagaagaa ctaggcgaac gcacagattt gtcaaaaggg tatatttccc
    12781 agttggaacg agatttaagt tcaccatcaa tggaaacatt cttttctatt cttgaagtct
    12841 taggtgtgac tccagaagaa ttttttcatc aagaagcagc gaatctgcaa gtggtttatt
    12901 caaaagaaga tcatacggtt tatttcgatg aagaaaacgg gtatgaattg gaatggctcg
    12961 tggctgattc gaatgaaaaa gaaatggagc ctgtcttgct gacttttgaa ggtgcaggag
    13021 aatataagac gtttgaacct tcattatctg aaacgtttat ttttgtatta gaaggggaat
    13081 tagagttaag cttaggagag acggtctatg tggcaaaagc aggacagtcg atctattatc
    13141 aagcgacaaa acaacatcaa ttaaggaatc atctcaaaaa acgaacaaaa gtattgattg
    13201 ttgcaacgca atcatatttg taatataggg ggtaaaaacg tggggaaacc aatcatttca
    13261 tttgatcaag tagtgaaacg ctatgatgat gaaacagtat taaaaaaagt aagttttgag
    13321 atcgaacaag gaaaatttta tacactgctt ggtccttccg gctgtggtaa aacaacgatc
    13381 ctacgtatta tagctggatt caccgaagct acagaagggg acatctattt tgaagggaaa
    13441 cggttgaatg atttgccagc taataagcga caagtaaata cagtcttcca agattatgca
    13501 ctttttcctc atatgaatgt ttttgataac gttgcttttg gattgaagat caagaaaatg
    13561 tccaaatcag atatcgaaaa gaaggtaaaa gatgctttgc gtatggtcca gcttcctgga
    13621 tacgaaaaac gggagattag tgaaatgtct ggtggtcaaa gacagcgtgt agcgattgca
    13681 cgagcaattg taaatgaacc gaaagtattg ctattagatg aaccgttatc ggcgcttgat
    13741 ttgaaactac gtacagatat gcagtatgaa ctgcgggatc tgcaacaacg attaggaatc
    13801 acgtttattt ttgttactca tgaccaagaa gaagcgttgg caatgagtga tgagattttt
    13861 gtcatgaata aagggaaaat cgtccaaagt ggtacacccg tagatattta tgatgagcca
    13921 atcaatcatt tcgttgctga ttttgttggg gagagcaata tcgtggacgg tgtaatgatc
    13981 gaagataatc tagttgagtt tgttggtaag caatttgaat gtgtcgatgg cgggatgcgt
    14041 ctaaatgaac cagttgaagt tgttttacgc ccagaggatt tgacgatcac gacaccagat
    14101 aaaggaaaac tagtcgtaac ggtcgatact cagctgtttc gtggtgtcca ttatgaaatc
    14161 atctgttatg atgaacagca aaatgaatgg atggtccatt caacgaaaaa agctaaagaa
    14221 ggaagcaaag taggattggc ctttgagcca gaagatatcc atgtcatgcg tttcaacgag
    14281 tctgaagaag aattcgatgc acgtcttgac agctacgaag aatagggggg acaagaatga
    14341 aaacaatgcg caggatctat tccgtccctt atattttgtg gctcggttta ttcgtgatcg
    14401 cgcctgtatt aatgatcatt tatcaatctt tttttgatat gaatgggcaa ttcacgctga
    14461 ataattatct atcttatttt acgtcgggga catcgctttc tatgacgatc aattctgtat
    14521 ggtatgcatt tttgatcacg tttttcacgc tgatcatcag ttacccgact gcttattttt
    14581 taacaaaact gaagcacaag caattatggc tgatgctgat tattttgcct acatgggtga
    14641 atctgctgct taaagcttac gcatttattg ggattttcag tgtgcacggc agcgtgaatc
    14701 atttctttga ttttatcggg atcgggccga aacagatatt attcacagac ttcagttttc
    14761 tgttcgtggc aacgtatatc gagattccat ttatgattat gccaattttc aatgcattgg
    14821 aagagatgaa tccttctttg atcagtgcta gtagagattt aggcgcgaat agctttgaga
    14881 ccttccgacg agtgattttt ccgttatcat taaatggtgt gaaaagtggt gtccaagcag
    14941 tatttattcc ttctctttct ttattcatgc tgactcgtct gattggcgga aatcgggtga
    15001 tcactttagg aacggctatc gaacagcatt tcttggttac acaaaactgg gggatgggtt
    15061 ctacaatcgg tgtgatttta atcattgcta tgttcatcgt gatgctcctg acaggcgaga
    15121 agaaaaaagg aggtcgctca taaatgaaaa aatttaaatg gtcgtatctt tatttaggga
    15181 tcgtcttttt attcttatat gcaccgatct tctatctgat tttttattcc ttcaatgaag
    15241 gaggaacgat gaacagcttt acaggattta cttgggaaaa ttatgaagct gtttttgaag
    15301 atacacgttt gatcattatc gtattgaata cgttcatgct ggctttctta tctgcattga
    15361 tcgccacgat catcgggaca tttggggcta tgggaatcta ttacacgaaa aaacgacgtg
    15421 cacggacagc gttgttgagc atgaataaca tcttattagt ctctcctgat gttattatcg
    15481 gtgctagttt cttgattttc tttacactac taggattcgg attaggtttt cagagtgtat
    15541 tgctttccca tattgctttc agcattccaa tcgtcgtctt gatggtcttg ccaaaattgc
    15601 aggaaatgaa cgattcaatg gtcgatgcgg ctagagacct tggtgctaat aatttccaag
    15661 tagtcaaaaa tatcattttg ccttatcttt ctccaggaat catagctgga tattttatgg
    15721 catttaccta ttctcttgat gattttgcgg ttacattttt cgtcacagga aatggcttta
    15781 cgacgctctc agtagagatc tattctcgtg caagacaagg aatcagttta gaaatcaatg
    15841 cgctaagtgc gttagttttc ttgttctcca tgatattggt cattggatac tactttatta
    15901 gtaaagaaaa ccgtccaaaa cggctgaaga aaaacagaag agtacgcgag ggggtggcac
    15961 ggctgcgatg aaaaaattac aatctctgtt tttaggtatt ctcatcatta ttgcggtgct
    16021 gctgcttggt gttcgtcaac ttgaaaaaac aagtggcatg agtggagcaa aggtacttac
    16081 catctataac tggggagatt atatcgatcc tagtttgatc acgaaatttg aaaaagaata
    16141 tggatacaaa gtgaattatg aaacatttga ttctaatgaa gcgatgttca caaaaatcca
    16201 gcaaggtggg acaaactacg acatcgctat accaagtgaa tatatgatcc aaaaaatgat
    16261 caaagaaaaa ctagtacttc cattagatca ttcgaagatc aaaggattaa ataatattga
    16321 tccacgattt ttagacttgg attttgatcc agataatcag tattcgattc catatttttg
    16381 gggaacttta gggatcgtat ataacgataa attctttgat gcaaatcaaa ttaagcattg
    16441 ggatgatttg tggaagccgg aattaaaaga cagtttgatg ctgattgacg gtgcacgaga
    16501 agttatgggg ctttctttga atagtttagg ctattctttg aatagtaaga atatggcaca
    16561 gctgacagaa gcagcaaata aattgaatca tttaacacca aatgtcaaag caatcgtggc
    16621 agatgagatc aaaatgtata tgatcaatga agaagcctcc gtggcagtga cattttctgg
    16681 cgaagctgcc gatatgatgt gggaaaatga acatttacat tatgtgatcc caccagaagg
    16741 atccaatctt tggtttgata atatcgttat gccaaaaacc gtgaaaaata aagaaggtgc
    16801 ctatgatttc atcaacttta tgctagaacc ggaaaatgcg gcacaaaatg cagagtatat
    16861 tggttattct acaccaaatg aagaagcaaa agcgttattg ccaaaagaca tttcgtcgga
    16921 cgaacaattc tatccatctg atgacacgat ctcccatctt gaagtctaca aagatctagg
    16981 atcgaaatac ctaggcatct ataatgacct attcttagaa ttcaagatgt ataggagata
    17041 atggcaaaac tcagtaagaa tggggactta tcaacgttct aaagagctaa aaatcgtcct
    17101 ttttctatct gagttttcca atcttctacc aaaagcggat gaaacgccgc tttttttgtg
    17161 ttattttatt ttttctacca cgtttttcta ccagagaaaa gtggacaaaa ccattgtaaa
    17221 atagggcccg agacgggcta gtaaatctaa gattgaaaaa gttacagaag gataaagtaa
    17281 ttggaaacta tgcaaatata cttaaccctt ctgtaatcta ttaataaaac taatatatga
    17341 atccagttca atatttgtag ctatgaaaaa acataaaaat catattagat aaaaactaaa
    17401 ttggtagata aattattttg tcagatatct gaggcaaata ttcgactatt tgtgtttcaa
    17461 agtaaaaatt ggaatacaaa tatagaatag acatattgca gccgatagtt tattatgctt
    17521 ttagacacat taaactggag gaaaaaatga aaaagagcgc taaagatcga gtttttgatg
    17581 agctgatacg actaacaggg aatatgtctt ctgtcactgc cgagaaaatt tcgaagaatc
    17641 taggtatctc tcgtcagaat gccagccatt atttgacgag attagtggaa gataaaaagg
    17701 tcgagaaatt accgggaaag cctgttttgt ggaagccttt agatgaatat gcggttgtag
    17761 ataatacgga gcaaataaac gaagcctttc attctgtagt tggtcatgac ggaagtctga
    17821 gagaggtcat tcaaaaatgt atagccgctg tcaaatatcc gcctaacgga ctcagtgtat
    17881 taataaatgg agccacgggt gttggaaaaa gttttttagc cacaaaaatt tttgaatacg
    17941 ctatccatga gcaaattatt gaaaaagatg caccatttgc tatcttaaac tgtgccgatt
    18001 atgcggacaa tcccgaactg ctttcggcaa cactgtttgg atacaagaag ggggccttta
    18061 cgggggctga aaaggatacc gatggtttat tggcaacggc caacaacggc tatctttttc
    18121 tcgatgaggt ccatcgccta tcgaaagaaa atcaagaaaa gctctttcta tttattgata
    18181 ctggaaacta tcgtccagtc ggtgaaaatg taaattggca ttctgctaag gttcgtttta
    18241 ttttcgccac gacggaaaaa ggtgaaaatt atttattaga cacctttgat cggcgtattc
    18301 aaattagtgt tatgttgcca acttttgaag atcgccccat tcgagaacga ttagagctga
    18361 ttcaattatt ttttcaaaat gaagcggatg ttttacaaaa agatattatt gtttcaagag
    18421 aagccttgtc tttaatgtta tctcatccct ttagtgggaa tattggtaag ctgaagaata
    18481 tcattaaaat cagttgtgcg gatgtgtata gtcgaacaca agaagaacca ttaacgattg
    18541 ggaaaaatga gatcttgatt cagttaaata tgctggaatc cgaagtagaa agcttgccga
    18601 ttgctagttt attgataaag aaaaatcaaa agataaaaac ggatcgaatc aaaccgtttg
    18661 attatgattc gattttggaa aaaattactc agctgataaa tgaaggtaat ttcaatgaaa
    18721 atttttcttc gataaagaat gaagtacagc atttggtgca tcacattgat aaggattctt
    18781 ttttatcaat gagtagaaaa ttgatgattc agatgtttag aaaggtctgg gtatccgttt
    18841 taggaaaaac gtacggattg acgacgagtg gacctgttgc agaaattatt agcagatcct
    18901 atgtcatttc cccaagctat aagacagact taaccgggat aaatcgtaca ttagctgcaa
    18961 atctacctcg gacgacttac ttatgtaatc agttcttgga gcatttacca ccgctctcag
    19021 aaaaggacga gaactttcta agactgctta ttgtcgtgac tttatctgat tatgtagatg
    19081 aatccatcga gctgaagggc ctgttgcttg ctcacggaga tagtacggct agcagcattc
    19141 aagcagtagt gaatcaatta tgcggcaatt atgtttttga agcgttggat atgccagtag
    19201 agagtaatgt gtcagagatt gtcgaatcga caaggaattt cgtgaaacgg caagtgaata
    19261 cagatagtct agtgctgatc gtagatatgg gctctttaaa tcaaatttac acacagatca
    19321 agaacgattt aaaaggggag ttacttcttc tgaataattt gacgacttcc attgcacttg
    19381 atattggctt gaagatgacg atgaatgcgc catttaaaca gattgcggag gatgcagcag
    19441 acaagtattc aatcaatgtt caatactttg aaggcttttc tcaaagtagc aacattatca
    19501 tttcctgtat gtcgggtttg gggatttcgg acaaattgaa agatgttttt cgtcatcatt
    19561 tatcggatga atccatcgaa gttttcacga aggactacag agaactacgt gaattaatcg
    19621 accaaaatga tgaaacttat tttgacaaga caaaattggt catcacgact tcggatcttc
    19681 cacgttcctt ttcaattcct aatattaatg tttatgacat cttagacggt gatggagcag
    19741 ccaatttgga gcatattttg agtcctgaaa tgaagccggc agaatttaac cgcttgattc
    19801 aggagctagt aaaattcttt tcgttggaag gaattgcgga ccgtttgaac tttctgaacc
    19861 caacgatcgt gatgagtgaa gtggaaacag tcatttctaa atatgaaaat tactatcatt
    19921 tcactttaaa tggccgtgta aaactaaatc tctatatgca tacggcattg atgatggaac
    19981 gtttattctt ggctcggaca gaaaaatctg aagaaccgaa ttatgatttg aaaccagatg
    20041 aagatgaatt ttattatgtt tctagaagta tttttcaatc aatggagatg aaatataatt
    20101 ttaaagtcaa tgcttatgag ctttcgctgc tctatgaatt attaggaccg tatattcaaa
    20161 aataaagtgt ctaaaaaagt gtctaatctg ttttttttag gttagacact ttttttaatg
    20221 tgaattaacg tgattaaacc aatgttttta aaagttggca caggaattgc ttatataaaa
    20281 gtgaacagaa agaaaggaat taaccatatg aagaaaattc taatagcgac tcacggtcac
    20341 ttagcttcag gctttcttag cagtattcaa ttactgacgg ggaaggttca agagattacg
    20401 gtgattaatg cttatgtgga tgagtgtgat tttgaacagg agttgaatgt atttgtaaac
    20461 caggtgaacg agcaggatca gatttttgtt tttaccgatt tatttggcgg aagtgtcaat
    20521 cagaagatta ccaaaacctt tttggagaaa gagattgctg taactatttt gtctggcttt
    20581 aatctgccaa ttctgctcga aatagtattg gcaaccaagg aattaacaaa ggaggatatt
    20641 caggaactgg taaataaatg ccaacaggaa ttaaagatga ccattcttga tgaggtacat
    20701 gtagtaaatg aagaggaaga ttttttttag gaggaagaaa aatgattaaa gcagtgcgaa
    20761 ttgatgaacg attaattcat ggacaagtag cgatgacttg gacaaagagt ctaaatttaa
    20821 ccgggttagt agtagcaagc gatgaggcgg ctaccaacga tattcaaaag atgacattga
    20881 agatggctgc accatcgaat gtcaaagtca ttattaaaac agttgatgac gcgattcatt
    20941 tgttaaaaga tccgcgggcg aaggacatgc gcttgatggt tttagtccct aatgtcaaag
    21001 atgcggtaaa agttgcgaaa gctttcccaa cggaaatcga attaatgaac gtggggaatg
    21061 ctgggaaaat gacaccgcca tcggaagata agaaaacctt atcaaaggaa gtgatgttaa
    21121 cgacgtcaga aattcaagac ttaaaagaat tagttgaact ttatccagat accgtttttc
    21181 agcctacacc ggcgatggaa aaacgttcag ctatctctat tttgagtgat ttttagaaaa
    21241 aggaaggaga aaaattatgt atgatgcatt tgtagtcgct ttggccgttt ttattggagt
    21301 tgctggacat gaaatgttcg ggatgtccat gctgagtcgt ccgattgttg ttgctccgtt
    21361 agcaggagcg ttgatggggg atttgcagac ggggttgatt gtcggggcgt cactagaagc
    21421 catctttatg ggtgtcgtca atatcggaat ctctagtacg gcggagcctt ctttggctgc
    21481 tgggctggct acagcgttcg caatcaaaat gggaggaaac gtggatgcga ttattcccat
    21541 tgtctttcca ctagcagtat taggattaca gttaatgaat atgatttttt cttttgtatg
    21601 tggaccgatg gctgctaaat ttttcaaatt tgcaaaatct ggtgatcaaa aaggaatcat
    21661 caatctgcac ttctttatgt ggtttgtcca ctatgcgctt tatgctctta ttccattctt
    21721 tgccgtttta tttggagcag atgttgtcaa aagcgtcttg gataatattc cagaggtcat
    21781 catgaacgga ttaacagtcg ctggaaatct gctgccggca gttgggatgg cgatgctgtt
    21841 acggatgctt tggagtaaaa atattgctgt gtggtttttc tttggggccg ttgttatggc
    21901 gtatctcgag ttaccattga ttgcaattgc cgcagttgga acgattattg caattgttat
    21961 agcccaaaga gatctacaac taaaaaatat atctgttcat gcagttcaaa gcgatgctgc
    22021 tgtaaatgca acgcttgacc aagaagagga ggatttcttt caatgaaatt agctgatgga
    22081 atttctaaag atgagaaaaa attggttcgg aagatgtttt ggcgctcgat gactatgtat
    22141 gcttcggtga atccggtgac gatgggcggc ggtggtttcg cctattctat gatgcccttc
    22201 attaatcatt tctacaaaag tgaagaagat cgagctgcgg cgctggaacg tcatacacag
    22261 tatttttcga cgaccattcc ttgcgcgtcc tttgtcatgg ggattgcggc atcgatggag
    22321 aaggaaaatt ctgaaaaagc tggttttgac gtggaaagta tcaacgcgat caaaacgagt
    22381 ttgatgggac cattggctgg aattggcgat tcattgttct ggggagtttg gcgtgtcgtt
    22441 accgcgggac tagcgatcaa cttagccaat gccggaaata ttttggcacc aattatcttt
    22501 ttagttatgt tcaatatacc taattacttg atgcgatact atggcggcgt tttaggctat
    22561 tcattaggct ctaaatatat tgaaaagctg tatgcgaacg ggttaattga aattctgaca
    22621 aaagctgcta gtatcttggg tctgatcatg gtaggtgcaa tgacgatcag tatggtcaaa
    22681 ttcaataccg ttttgagctt ttcaatgaag ggcgaagaac taatgaatgt ccaagaaatc
    22741 ctcgatagta tttttaaagg aattgtaccg ttattggtaa cattgagctg tttcaaatta
    22801 ctagataaaa aagtcggcgt catcaaaatt atttttggaa tggtcgtctt gggaatttta
    22861 ttgtcattac ttggaatcgc atagtaggaa gctgcagcta ctaggctgca gtttttttag
    22921 caaggaggga aaagatgact attaatgaag taatttattc acccaaacac ccatttttta
    22981 atgctgagtt aaacgacgga aatgtgacta cttcaaacga atacagctac tatcggaata
    23041 ttcctgatgt aaagggattg accttcgatg taagtaatca aaagacatta gcaaaccttg
    23101 atgtttgcgg gacgattcaa acgttgacct tttatcgaga aaatttacta actgaggaaa
    23161 aaccaggtgt ttgggtcaat aaacagctct ctcaaaccaa ttcgttatcc ttaaaggttg
    23221 aagtaaacgg gattgtctac catttgtcag aatcggatca tcgtattgaa gtcgatgtga
    23281 ttcaagactg tttgcctaga tatattcatc attattcgac gtttaaggtc attgtagtat
    23341 cctttgctcc catactggct aataagcgtt taagtatgct tgttcaacag gtttacattg
    23401 tgaatgaaac acacgaaagc cttcaagttt cagtgaaaga tgtgccttcg tatcaagaga
    23461 agtattcgga tcagcaaaat gtgttgattt ctcaaaaagg aaataaggac acgatcgcac
    23521 aacaggaagt ggcaagcttc gccattgctt ttgtggaccc aaatgctttt gaagaagtaa
    23581 tgacttttca ggaatctgat atcgaaaaat ggctgaagga aacattgaac tattttgctc
    23641 agctatttgg gcagctgtca atgccaaacg atagagttgt ccatctctat cgtcgtgcac
    23701 tttatcaaag cttttctagt ttcggtatga atcgacaggg acaggtcgtc ggcagcaatt
    23761 ggggcagcta tcctgctact aatcgaattt ggaataagga tatgtattac gcatcattgc
    23821 cattcaccat gtttgaaccg gagttatgtc aaaaaacggt tctatggttt gatcaatatg
    23881 gcgttaagtt tcctggttca aaatttacag gaggtatcaa tcattctctg agtaattcgt
    23941 tatccagtgc gttactggct agtctttatt ttgaacatac tggggataca aaattctttg
    24001 agcggtgtcc gcaggttcta ctgaatgctt caagaattat tgaggacata ttagctcaac
    24061 gtcggtcagg ggaaccaatg ctttttccat ctgtttggtt atcagacgcc tttgcacttg
    24121 gaaagtatca taccggcagc aatctttgtc tttggaaggc ctgtattgga cttgcagaaa
    24181 tttttgaagc cctagaacaa ctgagtctta gcaagcgata caaaaacatt gcctgtaaac
    24241 taaaggaatc aatttgtaaa caaatgacta tcgatggtcc ttttggtgag caatttttgg
    24301 agggaatcgg tgatgaggag aaaacaacct attcagtgaa gcactatcaa aaaccgattc
    24361 ttgagcaagg gttaatcttt ttaagtgatg tgattgtgga tggaaaaatt aatttattga
    24421 tgcatgatgg agaagaatca gacactacgt tgatgccttt ttatcaattt ttagataata
    24481 aagatcattt atatcagaac accatggcct tttctgctag ttctttcaat ccaacctata
    24541 gcgaggaaat caaaggcatc acttggggat tagaatccgg cgccactttt cctggcttta
    24601 tcactgtttt aatgagcgag ctgcacaacc ctcaagcttt cgtaacccga ttagaagaac
    24661 taatttgttt ggcagattta gacggttcgt ggtggtggtg gccgtatcgc ttagaggctc
    24721 aacaaggaga agtcgtgcga gactttggct gcggcaaatg cggctgggca tcgggtcttt
    24781 ttgtcagcct aatgatcagt caatatttcg gattgaaatt caaaaaagga gtattgcaaa
    24841 ttgatcctgt tgataattta acgttttctt ggaagaattt gaagttcggt caagccttca
    24901 tttcgattga atgtacgcaa tccgagttga gtatttcaaa tttgtcagaa aagccattga
    24961 aaataagtga tacgaaaaaa attcttcacg tagaaaaggg aaaaacgatt cacattcaaa
    25021 ggagaaaaag atgagtatac taaaaaatat tacccaattt aaaaaaatcc agagtcgagc
    25081 aatcactccc gaaaatccaa caggcgagaa aggtaaagct gcgatgttgc caagcgagct
    25141 aggaccaagc cgcaaaggtc gtggcgcaat tccgctgact tctggtgaag aaactgtgat
    25201 tgcggatatt actggaacgg gaataattcg tcatatgtgg atgacgattc gtgatcgaac
    25261 agaatttggc agttttgttt tacgtgattt aatcttaaga atttattggg atgggtcgga
    25321 tagccctgct gttgaaagtc ctttagggga tttcttttgt aatgggtttg gagagcgttg
    25381 cgaggtgaac tcgttgccga tagtggttaa tcctacaggg ggctttaatt cctatttcga
    25441 aatgcctttc caaacatccg ctaaaattac gatcgagaat ttacatccga aggatatgtc
    25501 ctcatttttt tatacgataa attacgcttt ggtggacgaa ttggaagaaa atagcctata
    25561 ttttcacgca aattggcatc gggaacgcat taccaaatta caggaggatt ataagttaat
    25621 tgatcgatta aagggtcaag gctattacgt gggtacttac cttgcgctta cctgcctaga
    25681 acgttattgg tggggcgaag gcgagtttaa attctacatt gatggggata gtgagtatcc
    25741 gaccgtaacc tcaacaggtt cagaagatta ttttggcggg gcgtgggcct ttcatgagta
    25801 cgatgagttc ggacgtccac atgcgaaaaa ctattcaaca gcctttttag ggtatcctta
    25861 ccaatcaaat agagatgcca caagagatcg ttttgaaacg ggaaaattga atgccgttca
    25921 tgcctttggt gacgacgcaa tgccacgaca tggtctttat cgttggcatc ttcaagaccc
    25981 catctgtttt accgaagatt tatcggtcac tttacaacaa atcggcaacg atgatgttcg
    26041 cttatttgag cggcaagacg atgttgctag tgtcgcttac tggtattcaa cagaagcttc
    26101 aggaaatact aaacgtcatt tcactgatcg agaacgcttg ccgcgttaat gaagtgtttt
    26161 tcacatattc gaagggagga acctatgatg atatatgggg caattgaagc tggagggact
    26221 aaatttgtct gtgcagtcgg tactgaggaa ttaacgattt tggaaagagt cagttttccc
    26281 acaacaactc cagttgaaac aatgaagcag gtcattgatt tttttaaacc ttttaaggaa
    26341 caattggcag gcatcggggt cggctgcttt ggtccaattg atattcaatg caaatcaacg
    26401 agtttcggtc atatcacatc aacaccaaaa cttgcttggc aaaatttcaa ttttgtagga
    26461 acgctaaagc aatatttcaa tattccgatt gaatggacaa cagatgtcaa tgcggcttgc
    26521 tatggtgaat atgttgctgg ttctggcaaa gggttagcag gtgttgcata cttcactgtg
    26581 ggaacaggta tcggaggtgg tgccttgttc aatgggactt tcgtggaagg gttcagtcat
    26641 ccggaaatgg ggcatatgtt gatcaagcga catccaaagg acaatttttc aggaaactgt
    26701 cccttccacc aagattgttt agaggggatg gcagcaggac cagcgattga aaaacggttg
    26761 ggtgtaaaag gccagaattt attggctgac gattcgtttt ggcagattga agccttttac
    26821 cttgcccaat gtgcatacaa tacaacactg atgttttcgc cagatagaat tatttttggc
    26881 ggtggtgtaa tgaaacaaga acacatgaag aaaaaagttc aggataaatt tgttgagctt
    26941 ataaatggat atgttgaaat accaccaatt gatagctaca ttattacgcc cgaattaggt
    27001 gataatgccg gaataatcgg cggattagca ttggccagaa aagcggtaag aaataagcaa
    27061 ccataattaa tgaatgtttc attgatactc atgtcaaaag tatttaaccg agtaatcaaa
    27121 tgatcaaatg aagttggata aagcgaaaat gaattcaaat aaaaaggctc taagtcaaat
    27181 cgtgttgttt tcattaaatc actgaaagga aatgcttctg cgtttccttt tttctttttg
    27241 ttgtgcgctg tataccttga ggatattcac tggtggcgta cacttaaata gtaatatatt
    27301 gtgttacttt atgtctgtgg taatagttat agttgcttac caatgtatgt tcatttttcg
    27361 aaatgttgac ttcataatcc ttggcttaat gtttgaattt tt
//
LOCUS       DACDUU010000035        27394 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.035, whole genome shotgun sequence.
ACCESSION   DACDUU010000035 DACDUU010000000
VERSION     DACDUU010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.035"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..714)
                     /locus_tag="IWL30_001503"
     CDS             complement(<1..714)
                     /locus_tag="IWL30_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9706270.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVN"
     gene            980..1324
                     /locus_tag="IWL30_001504"
     CDS             980..1324
                     /locus_tag="IWL30_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAP9706271.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            1325..1744
                     /locus_tag="IWL30_001505"
                     /pseudo
     CDS             1325..1744
                     /locus_tag="IWL30_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1836..2186)
                     /locus_tag="IWL30_001506"
     CDS             complement(1836..2186)
                     /locus_tag="IWL30_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706272.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(2152..3483)
                     /locus_tag="IWL30_001507"
     CDS             complement(2152..3483)
                     /locus_tag="IWL30_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAP9706273.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            3739..4305
                     /locus_tag="IWL30_001508"
     CDS             3739..4305
                     /locus_tag="IWL30_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9706274.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            4450..4537
                     /locus_tag="IWL30_001509"
     tRNA            4450..4537
                     /locus_tag="IWL30_001509"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4482..4484,aa:Ser,seq:cga)
     gene            4871..5845
                     /locus_tag="IWL30_001510"
     CDS             4871..5845
                     /locus_tag="IWL30_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP9706275.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            6170..8065
                     /gene="asnB"
                     /locus_tag="IWL30_001511"
     CDS             6170..8065
                     /gene="asnB"
                     /locus_tag="IWL30_001511"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAP9706276.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(8260..8706)
                     /locus_tag="IWL30_001512"
     CDS             complement(8260..8706)
                     /locus_tag="IWL30_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP9706277.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8934..9083
                     /gene="rpmG"
                     /locus_tag="IWL30_001513"
     CDS             8934..9083
                     /gene="rpmG"
                     /locus_tag="IWL30_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP9706278.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            9171..9689
                     /locus_tag="IWL30_001514"
     CDS             9171..9689
                     /locus_tag="IWL30_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9706279.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(9766..10722)
                     /locus_tag="IWL30_001515"
     CDS             complement(9766..10722)
                     /locus_tag="IWL30_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9706280.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10888..11694
                     /locus_tag="IWL30_001516"
     CDS             10888..11694
                     /locus_tag="IWL30_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9706281.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            11691..12680
                     /locus_tag="IWL30_001517"
     CDS             11691..12680
                     /locus_tag="IWL30_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP9706282.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            12677..13681
                     /locus_tag="IWL30_001518"
     CDS             12677..13681
                     /locus_tag="IWL30_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP9706283.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13813..14355
                     /locus_tag="IWL30_001519"
     CDS             13813..14355
                     /locus_tag="IWL30_001519"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAP9706284.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            14375..15073
                     /locus_tag="IWL30_001520"
     CDS             14375..15073
                     /locus_tag="IWL30_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP9706285.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            15094..15306
                     /locus_tag="IWL30_001521"
     CDS             15094..15306
                     /locus_tag="IWL30_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAP9706286.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            15306..16268
                     /locus_tag="IWL30_001522"
     CDS             15306..16268
                     /locus_tag="IWL30_001522"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAP9706287.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            16286..16681
                     /locus_tag="IWL30_001523"
     CDS             16286..16681
                     /locus_tag="IWL30_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP9706288.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16844..17209
                     /locus_tag="IWL30_001524"
     CDS             16844..17209
                     /locus_tag="IWL30_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAP9706289.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            17206..19017
                     /locus_tag="IWL30_001525"
     CDS             17206..19017
                     /locus_tag="IWL30_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAP9706290.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTNENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(19079..19246)
                     /locus_tag="IWL30_001526"
     CDS             complement(19079..19246)
                     /locus_tag="IWL30_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAP9706291.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            19494..20483
                     /gene="galE"
                     /locus_tag="IWL30_001527"
     CDS             19494..20483
                     /gene="galE"
                     /locus_tag="IWL30_001527"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAP9706292.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            20495..21979
                     /gene="galT"
                     /locus_tag="IWL30_001528"
     CDS             20495..21979
                     /gene="galT"
                     /locus_tag="IWL30_001528"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAP9706293.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            22003..22983
                     /locus_tag="IWL30_001529"
     CDS             22003..22983
                     /locus_tag="IWL30_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9706294.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            23072..23896
                     /gene="tsaB"
                     /locus_tag="IWL30_001530"
     CDS             23072..23896
                     /gene="tsaB"
                     /locus_tag="IWL30_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAP9706295.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            23880..24422
                     /gene="rimI"
                     /locus_tag="IWL30_001531"
     CDS             23880..24422
                     /gene="rimI"
                     /locus_tag="IWL30_001531"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP9706296.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            24429..24893
                     /gene="rimI"
                     /locus_tag="IWL30_001532"
     CDS             24429..24893
                     /gene="rimI"
                     /locus_tag="IWL30_001532"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP9706297.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            24890..25906
                     /gene="tsaD"
                     /locus_tag="IWL30_001533"
     CDS             24890..25906
                     /gene="tsaD"
                     /locus_tag="IWL30_001533"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAP9706298.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(26515..27216)
                     /locus_tag="IWL30_001534"
     CDS             complement(26515..27216)
                     /locus_tag="IWL30_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP9706299.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT         8830 a         4695 c         5681 g         8188 t
ORIGIN      
        1 attgacatta ctgatctgtt tccaatttga aaattccttt ccacaatcaa aagtgatgga
       61 tttgaatagg tttttcggta cagattcaaa ccattgattt aatttttttt caatatcaat
      121 cgcttgtcta ccacacggtt tcaatgtgat gataactttt gataatcgtt caactaaggt
      181 aattacagca cttttatgtt tcagacctac gatagtgtca ccttcaaggt gaccaaactc
      241 atttgagaat tggctataat ccttttcacg ttcatgaata gagcggcgga aagtttgttt
      301 tccacgtctt tcttgatgtc cattcggttt acgcttgcct ttcatcggta agtcagaaga
      361 atcaaatagc ccctttttga acatacgata aatggtacga gcagaacaac taataggaaa
      421 cgctgcacga ccaacaatca catcgggtgt ccatccttga acaacctttc tttgaatata
      481 ttctgattgt tcctcgggta aaacaagcgg acgtctacca cagtttgatt tgtttttctt
      541 atactgttga taatattcta aggctgattt tccttgcttg aagaacagat atactttatg
      601 gatcgtttgt cttgaacgat tcaagcaatg ggcagtttta gcaacagatt gattcatttt
      661 gaaataagac tctattatta caagttcatc cgttgtaaga tgggtatagg tcatttgtac
      721 tcactcctta taattctttg gtcggaacta ttttgagtgt agcacaaatg acttttttag
      781 ttgtctagct taattttaca atcggcgaat aaaaaaagcg ttagcaacta tgcttaaaaa
      841 gcatatcgac tgaataaact aagcattgga caggctgaaa gactacattt aaaatgaaat
      901 gcgaagaaac aatagaaaat aaagttgcct tagaaccata gttgaatcac ctaacagtcg
      961 tgaagggaga ataagagaaa tggcagaaaa acaaaccgca ggacgagatc aattaggaga
     1021 ttttgcacca aaatttgcag aattgaatga tgatgtttta tttggacaag tatggtccag
     1081 agaagaatta tctgctcatc agcgtagtat catcaccata tctgcattaa tttcaggagg
     1141 aaattttgaa caattgccag ctcatctgaa gattggcaag caaaatggca ttactagtga
     1201 agaaatagca gaaatcatta cccatttagc tttttatgta ggatggccaa aagcttggtc
     1261 agcttttagt ttagcaaaag aaatctattt agaagaaaat agcgaagata agaaagaggc
     1321 gtaaatgacg acaaaacacg aagaaataca caatggcgtg atttttccag caggtgaaaa
     1381 aaacgaagct tacgctcaag tatttaccgg gtaaagctat ctgaatatgt tagtgaatga
     1441 tcccgaagtt tctgttggtg taggtaatgt aacttttgaa ccaggttgtc gcaatcactg
     1501 gcatgtccat aaagacggct atcaaatctt acttgtgacc ggaggagaag gctggtatca
     1561 ggaagaagga aagccagcaa gacgattaat ggctggagat gtcgtcgtaa cgcacaaagg
     1621 gattaaacat tggcatggtg cgacaaaaga cagttggttt gaacatttgg cgatcactgc
     1681 tggtaattca gagtttttag aacctgtaac agatgaagtg tatgaaggat tggaaaatca
     1741 ataatgtcaa gcttgaacag ctcctgaaag tagattttaa tcgaactttc aggagctgct
     1801 tttttgattc agtcttttat aaatagaaaa tagggttaat aataggaatg agaagcacgt
     1861 cgaaagatct taatattgat aatcggctgg gcatacatga tttccacgtc atcaatcaaa
     1921 ctacctaaca gatgataatc ttcccgatca tgtcggggat tacctaatag ttcttgataa
     1981 tagatttcca tactagggtc ccgaggattg ttcagcaaat cagcaagtac atctaaatct
     2041 tctggctgag tttctgccgt accctgtatc tctatgaaaa gcccttcctc agcaaattct
     2101 actccaatac gtatttcagt tatttcatgg gaatataaat aagtaagaag ctcatcgatg
     2161 attctttttg ataattcttt ttccaagtgt ctttttttcc tttctgacag cggtaaagtt
     2221 atctagtatc ggtacaagga tacctgcaac gaaaccagtt gaaaaaccac tgttatatag
     2281 atttagtcct ccgtgcaaag tgctgacgtt atggacaaga gcaatatgta gtacaccagc
     2341 tattacaccg taaaaactgc cgtaaaaacc gcttatgggc gcgagacctg tcccaaacac
     2401 agctgcaaca agcatgccag tactggtgat ctcatgaaaa gttccaaaca aagaagcaat
     2461 aaatattccg actagtatcg gaaaactgtt ttttaaatga caaccaaaag cactgaaacc
     2521 caccgcagat aaaatagcac cgatgacagg tccatttaac tgaccgccaa ccagcaaaac
     2581 ataagaagac agcatcagac caactaagcc tatgttgatc aaagtagcac caataccaga
     2641 attagcaatg aaatcagttg tcaattttcc agatgaatcg aaaatttctc gaatccccaa
     2701 aagccgaaag gaattataat agaaaccaat tccgactgtc agcgaaaaaa agccaaaaat
     2761 aatccaaatg agaggaaagt gatattcatt gaaaaccaat gtattttcga cgattgaata
     2821 gctgaataac ctcaaaacag cagtaaaagt catcgcaatc aacccagagg tgaatccaat
     2881 attataaagt gtgaatccac gatggaaaac taaagtttga gcagctaagg gcggaagaag
     2941 tagaccaatt gctatgccag ctaaattccc caataaaata cctatcagta aaggaaaacg
     3001 cataccaaaa gtgatataac taacgaccgg tgataatgcg ctaccaaaca gtccgatcat
     3061 gatgtactga gaaaaaggtt tattgacaaa tacagcgtat agatacacac ctaaaatgat
     3121 tgagatagaa ttataaaggt ttttgccaaa aaaagagaaa ccggtaactg ttagaatcga
     3181 agcaaataag ggaccgttta aaatcacctt gtgccgataa gctaaaagca cagaaagtaa
     3241 ggtcaaaatc cctacattta tgaatgcgct tccgaaaccg cctaaagcaa aataatctgt
     3301 caaaagattg cttggcgagg taagaatagt tagcaatcca ctccaattat cagtaggagt
     3361 attagaaagc aagccaataa aaagaaaaaa aacacctgta aaaccaagat aaaaatactt
     3421 tcttttctgt gaaacgattc gtttttgagg atgaatataa ataacgtcag tttcgttttc
     3481 cattacacac cttctttttc attttcttta actataactt gaaatgaaag atttggacaa
     3541 ctatcattta ggtcaaaact aaaaatgtaa gggaacccat tgaatttaac caaatttttt
     3601 taattttgca aaaaaaagtt aaagaagact gaaaaaaaat ttaaaatata acgttttgag
     3661 taaaatgttt gcaaaaaatg ctaaaaatag ggtacaataa aacaacatat actgcagtaa
     3721 agaagtgggg gagttttaat gagaagaaaa gtctatacca aggatcaaat tctaaaagct
     3781 gcttatgatg ttgttgcaaa ggaagggttt aaaggattta ctgcgagaaa tattgctaaa
     3841 aagatgggga tttcgacaca gccgatttat ctggaattca aaaacatgga agatttaaaa
     3901 aatacgttgc tggattctat ctttgaacaa ctaaaagaag atgttttccc tattgaacgt
     3961 actggtgata aaatcattga tctcggtttg aactatatct actttgcaaa aaagcatcat
     4021 agtctttaca tggcattgta cattgatgaa catggggacg gaaagaaaat gcatgatttt
     4081 tcatacaatt atttccgtga aatagttaaa gaagatgaaa gatacaatca attatcggat
     4141 gaatatgtcg atgctctgca tactggtaca tgggtagcag tgactggctt agcgtcattg
     4201 atgtcatcaa acatcatgca tccaacagaa gaacaaatca ttgcactgat caaacggaca
     4261 attgactcga ttttagcatt agataatcct gaaataatca gatagtgttc tatcagtatg
     4321 aatttgatcg gtaagtccgt tggctgcaaa ctgtcaatgg acttttttgc taaaaaaagc
     4381 gatttctgtt atttatctat tagaaatagg cttgacattt cgtgaaaaaa gtttaaaaat
     4441 agtacttacg gaaggttggc agagtggtaa tgcaccggac tcgaaatccg gcgaaccggc
     4501 taataccggc gcgcaggttc aaatcctgta ccttccttat atcaacgatt agaggttgtg
     4561 aagaagctgt cctgcatcaa gtaataagaa cggccaaaca aaaatagctt ttcctatttt
     4621 ttcgtttgat tgggcttatt accgcagatg caagcttttg aacaccgttt gttcggaata
     4681 aggggctgtg acaagacttt tgtcacagcc ccttattgtt gtacttttaa cctacaatat
     4741 tttttgattc cccctgagta ctatttttta taaatgctag taaatcaatc ttctttggcc
     4801 tttttttgat aaaaaaaata ctagatgtaa gataaaagtg taaaaacgat ttgttttagg
     4861 acgggaaacg atgtgtaccg caatcactta tgcaacgaaa gaccattatt ttggaaggaa
     4921 ttttgattat gagatgtctt ataatgaagt ggtcactatt actccaagaa attatcgttt
     4981 cgattttaga aaggtaaaaa atttagataa acattatgca atgataggaa tagcagctgg
     5041 ggtatccaac tatccactct attatgaggc tacaaatgaa aaagggttga gtatggcagg
     5101 actgaatttt cctggcaatg ctgattataa agaattacaa gaaggaaaag ataacgttgc
     5161 accatttgag tttattccat ggatactagg gcaatgctca accatagacg aagctaaaga
     5221 attattggct actatcaatc tagtcaatat cgattttagt gaaaaactac ccttgtcacc
     5281 tttacactgg ttattagctg ataaagaaaa atcgatagta attgaaagta tgaaagatgg
     5341 acttcattta tatgataatc ctgtcggtgt gttgacaaat aatccgccat ttgattatca
     5401 attatttaat ctgaacaatt atcgctcgct atcaaatgga acaccagaaa atcatttttc
     5461 aaaccagatc agcttgaatg tttatagtcg tgggatggga ggattgggct tacctggaga
     5521 tctctcttct gtttctcgat ttgtaaaagc cacatttact aaaatgaatg ctgcatcagg
     5581 tgattcagaa tctgaaagta tcagtcaatt ttttcatata ctaggctcag tcgagcaaca
     5641 aaaaggagta tgcgatactg gagaaagaaa atacgaatat acgatttatt cttcttgctg
     5701 taacgttgat aaagggattt attattatcg tacgtatgaa gacagtcaaa ttactgctgt
     5761 aaatatgaat aaagaagact tggatcgtca tgaattggtc agttatccag tcataaaaga
     5821 gcaacagata aactatgtca attaattaat gaatagatcc gtttggtatc atattttctg
     5881 atacagtcat aattagacac gatctttagg tcgtgtcttt ttttgctggt ttttttggaa
     5941 atagcatcca ctaatatttt tttaacacga agagaataag gtagcctcaa aaaagaaaac
     6001 atggtaaatt agatattggg actttatttt ctgtaaaaac agaaactttc atcttttgtt
     6061 ttcacgtgac gaaattgtaa gagagaaaat attcgtccgc tatttattct atgataagat
     6121 gcatatgaat gaaataaaca gtaattttat attaggatgt gaaagttaaa tgtgcggtat
     6181 tgtaggtttt gttaacgaca aggacaacaa aaaaacaatt attaatacaa tgatggatcg
     6241 aatcgttcac cgtggaccta atagttcagg agaatatatc gataaacatg ttgctttagg
     6301 attcagaaga ttaagtatta ttgacttaga gggcggtact cagcctattt acaatgaaga
     6361 tcggacaaag atcattattt ttaatggcga aatctataat taccagcctt tgagagaaga
     6421 attgattgca gctggccacg tttttcaaac acatgcagat acagaagtcc ttttgcatgg
     6481 ttacgaagaa tggggaacag aattattgca aaagatccgc ggaatgtttg cttttgctat
     6541 ttgggataat gaaaagaatg agttattcgg tgcaagagac cactttggga tcaaaccata
     6601 ttattatgca gaaatgaacg gaacatttat gtttggttca gaaatcaaaa gttttctgcc
     6661 tcatcctgat tttaacaaag aattgaacaa agaagcgttg aaaccttata tgactttcca
     6721 atattctcca ttaaacggcg aaacattctt caaagatgtc tatcgtttgc ctgaaggcca
     6781 ttattatacg tataaagatg gaaaaataga cattcagcaa tattgggatg cagattttga
     6841 aacaaaagaa acgcattccc gacaagaatg gatcgaaaaa atcgacgaaa ccgttcaagc
     6901 ttctattgaa gcacatacgg tcagtgatgt agaagttggt tctttcttat ccagcggggt
     6961 tgattccagt tatgtaacct ctgtattgaa accagatcat tctttctcaa tcgggtttga
     7021 tgataaaacc tataatgaag cgattgaagc cagaaaacta accgaactat tggacttaga
     7081 taatacagca gcagtgatcg acggagacat gtcgttcaaa gcattccctt tgatccagta
     7141 tcacttagat gaacctgatt caaatccttc ttgcgtacct ttgtactttt tagcaaatct
     7201 tgcttcacaa agtgtgcggg tagtccaatc tggagaaggg gcggatgaac tgtttgcggg
     7261 ataccaaacg tatggattcc atacgaattc aaaattcatc cgtgtgattg ctcaaggatt
     7321 gaaaaaatta ccaaaaggaa cacgctataa tttaggacgt aaaatcggaa aaatgaaaaa
     7381 tttccatgga cggatccatt tatatgaatc acttgctcct gcaaaagaat attttatcgg
     7441 tcatgctcga gtgttcgaag aatcagaagc atcagaagtg ttaacaccaa aatatcaaac
     7501 agcaccatct gtggatgaaa tcatgactgt tcattacgag aaaacagaag gaatcaaaga
     7561 tgaagtcaat aagatgcaat atgtcgatct tcatcaatgg atgccaaaag atatcttgtt
     7621 aaaggcagat aaactttcta tggcaagttc tttggaagtc cgagtacctt tattggatat
     7681 cgaagtcatg aaattggctc aacaaatacc aagtaagttt ttattgaatc aaaacaacac
     7741 aaaagatatt ttcagacaag ctgcgaacaa acatcttccg gaaaaatggt cgaatcgtgt
     7801 gaaattagga ttccctgtgc caatcaaagc ttggttgaaa gaagaacacg gctacgaaca
     7861 agtcaaagca ttgttcgaag cagattttgc aaaagaattt tttgatcaag aaaaaatcat
     7921 gaagttgcta gatgaccatc acgaaggacg taaagaagag caaagaaaaa tctggacaat
     7981 cttcagtttc ttgacatggt ataaagtcta ctttgtagat gaaacgattc cgcaagcaga
     8041 agcaatcgac tatgtgactg tttgatgaaa atcaaatatc aataaaaaag aggttttctc
     8101 ctaccgttta acactgagtg ataagaaaca atttcaaaaa acagctcttc ccgttttata
     8161 gatattatga cttatggttc ggagttggca gaggaacacc attgtttcga acataagggg
     8221 gttgtgacac gattttcgtc acaacccctt tacatatgtt caataatcga tttctttttc
     8281 ttttgtccca tcttttttat aaatgaacaa agttgattct ttgttttgtg ctacttgctt
     8341 tcctttttcg attgcttctt tttttgtatc aaaatggttg cttgcctttt ttgccccttt
     8401 agatttcacg atccattgtt cttcctcata ttctacgatc acatctgaat caagcagttt
     8461 tccagcacga ggattcgtat cgtgttcatc cgtcttgctt ggattttttt ctttttcgaa
     8521 agttcgtttt tctgattcac tagcatcttc ataccatttt tttgcttgat caatggcaat
     8581 tgggatcgca cggttatcag ggtacccttc atctaataat gcatttgcga tgtcgattgc
     8641 tttttttctt gtcaatttat ctaagttttt cattgatgca ggaaagtcct tcatgttcca
     8701 tggcatatta gtatccccct ttattaaaaa acataatcta tataaacaag tgtaagggta
     8761 tttatacaaa aagaaaaaga ttttgtttga gaagtaaaac cgttgtattt atcgtcaagt
     8821 agttttactt aacaaaatcg tgaaaaacac tagcaatcta gaaaaaatca tggtaagata
     8881 atcaagtctg agagtcatct tagaggattc ttgtaaaaat gggagggaaa aacatgcgcg
     8941 taaacatcac tttagagtgt acttcttgta aagaacgtaa ctatctaaca agcaaaaaca
     9001 aacgtaacaa tcctgatcgt ttggaaaaac aaaaatattg cccacgcgaa cgcaaagtta
     9061 ctttgcaccg tgaaacaaaa taatgttttt aaagttctca attccttgct ggtcaaagaa
     9121 ttgagacttt ttttatctca aaacatctaa aatcataagg agcggataat atgtttattt
     9181 ccatgtgggc acaagataaa aatggtctga tcggcaaaaa tggtttgtta ccatggcgat
     9241 tgccaaatga tatgcgtttc tttcgcgaac atacaatgga tcgaatttta gtgatgggaa
     9301 gaaagaccta tgaaggaatg ggggatcttt cacttcctta tcgccatatc atcgtgttga
     9361 caacgcagcc aaattttcgc acgaaagaaa atgcggaagt aatgcattcg atcgatgaac
     9421 ttttgtcgta tagtcaaacg atctcagaag atatctatgt ttcgggtggt agtcggattt
     9481 ttcaagaact tcttccttac actggtcgga tctggcgaac attgatcgat agcacgtttg
     9541 aaggcgatac gtatattggg aatattgatt tttcagactt cgcccttata gaggagtatg
     9601 aggggataac ggatcaagaa aatctatatg cccatcgttt tcaaaaatgg gaaagagtaa
     9661 aaaatagggt tgcttcaaaa gaagaataaa atctatgcgt ctagtttgtt aataagaaaa
     9721 aaagcatatc acgactaatc gtgaagatgc tttttttagt taaaatcaat ttagtttttc
     9781 tttgatatca ttgaccattt cctgactggc aataggtccg ttatatagcc aactagattc
     9841 aggtccgaat tgcagtacat gattgtctgt cactgcttta agattcttcc agcttggttc
     9901 attgaacatg gcagcatctt tgtcactgtt cacaaggatg atgtagtcag catctaattc
     9961 agctagcttt tcagaggaaa cagcagacca gtcagaagtg gcttttttgg agacctcttc
    10021 cactagatca ggaacaccaa attccagatc gtcgtatacg attcttccac ttgaccgatt
    10081 tttgctgacc ataaaggctg agttgttggt cacccacaac actgcagctg tcttgttctc
    10141 gattttatct gctagttctt ctcgagtttt cttgatggat ttttgataat cggaaatgat
    10201 cttttctgcc tctttttctt ttgccaaagc tttgccgaca tcttttagtt gttcttccca
    10261 tgtgacatct gttccgtttt ttacaacata agttggcgcg attttgctat attgttcgta
    10321 tttcccgcct tcgacagtag cggaagaaga aatcaacagc aaatcaggct caaagcttaa
    10381 cactttctca taaggaaggt cataagaaat cgttgggaca tcctttaaat catcttgcag
    10441 ataatcttga atggaaccac tgccaacagt ccactgggcg ataggttttt cccctagtgc
    10501 aatcagatag tcttctaagt aactgccgat gatccgtttg ggcgcgtttg gaatctctac
    10561 tttatgtcca agtgtatctg tgactgtatg agtactattg gatgaagtcg tcgaattggc
    10621 agttgtagct gtttctttat ctgaagaggg ggagcaagcg ctcagtactc cagccagcag
    10681 caatatgcta gataatgtga atacttttgt ttttaatttc atttctttcc tcctaacata
    10741 attgagaatc tttctcattg gttattattc ctgatatttc gaacgctgtc aaccgcttca
    10801 tttggctata gatatgaaag tagcgcttga cgaaatgttg aaacagcgtc ataattgaga
    10861 atgattttca gcgaaaggat gggtgaaatg acgagattaa gagcagctga cattgcagcc
    10921 ggctacgaaa acaaaaaaat cattgaaaac atgtctgtta cgattcctga agggaaaatc
    10981 actagtttga ttgggccaaa tggaagtgga aaatctactt tattgaaaag ttttacccgt
    11041 atactacctg tgaaatcagg aaagatcatt gtcaataact ctgatatctc tacatacagt
    11101 tcaaaagaac ttgcaaaaaa aattgcatta ttagcacaaa ctagtgaaca ccctacaggt
    11161 atgacagtag aagaagtagt atcttacgga cgataccctt atcaaaaact gttcagtggc
    11221 atggacacgc aagatcatga agcaatcgag tgggcgttgg atgctacagg tctagctgga
    11281 tttcgtatgc gggatatcgc aacgctgtct ggtggacaaa cccagcgtgt ctggatcgct
    11341 atggcgcttg ctcaggaaac agatatcttg atcttggatg aaccgacaac atttttagat
    11401 ccggcacacc aattagagat cctacagtta ttgactgaaa tcaatcaaat gaaacaaaca
    11461 acgatcttga tgtcgattca tgacattaat catgcttcac gtttttcaga ttatttatta
    11521 gcaatgaaaa acggtaagtt gatcacagaa ggtacacctg aagaagtgat cacaaaagag
    11581 tgtatgaaaa aaatatttga gattgatgca acgattgtca cgcttccaag aacaaataaa
    11641 ccagtttttc taacctatga tttgttgcca aaagagcagg aggatcaatt atgaggacta
    11701 tgaagaaaaa tagactcctg cttttggtct gttttgttct tttgattatt ttttttgttg
    11761 ctctttcaaa tggcgcagcg actgtgggaa aagcagattt ttggcaggcg ttgtttgatt
    11821 ttgatagatc aaaacaaagt caccaaatca tacgaaatat tcgtttgcct cgtgtggcgg
    11881 gagcatttct tgtagggagt tgttttgcat tgagcggtgc attgatgcaa ggagtgacaa
    11941 gaaatccttt agcagattca ggcttactag gaatcaacgc gggtgcttcg ttggcaatgg
    12001 cccttagttt tgctttttgg cctaaagctt ctgatttttc tgtgtttcta atgtcattgg
    12061 caggttcttt tgctgtaact ttgctcgtat ttggtttttt gcagttttcg aaaatgggca
    12121 tgaatccttt acagctgatt ttagctggcg tagctattag ttcttttttt actgctatca
    12181 gccaagcact tacccaattc tttaatctgc agcaggatct tgctttttgg tttgtaggcg
    12241 gagctgccaa tatcacatgg aaacaattag cagtattgtt tcctttatat atacttgctg
    12301 ttttggtttc tttttcatta ggaaaagcaa tatctttgat tcatttgagt gaaacacatg
    12361 cattagctct tggaagtcat cctaaacgaa cgcggttgat tgttttctgt ctagttgcag
    12421 tgttagcagc cattgccgtt tcgttagtcg gacctgtttc atttattggg ctgttggttc
    12481 cgccagttgt tcgcggaatg gttggaacgg actatcgtta tgttctacct gtctcttttt
    12541 tagttggcgg tggattggtt atgttggcgg attttacagc gaggatgatc aatccccctt
    12601 ttgaaacacc ttttggatta gtcattgcat tgattggcgt tccgttttta ctgatacaag
    12661 taaggaggga acaacggtga aaaaaatata ttggacgttc tatttcttgc tttttgtact
    12721 tgtcatcctg tctataggta gtttaatgat tggtacgcct tttatttacc tggatcagct
    12781 ttttgccacg ttcataggga aagaatcatc ttctttggcg ctgattgtta gagaatttcg
    12841 agttcctcga ttactgatca gtttattagc aggagcttgt cttggcgttt ctggttatat
    12901 cttgcaagga gtaacaagaa atgaattggc agattctagt atattgggca ttaatgccgg
    12961 agcaggattt ttagtgatgc tttatttagg tttcttttcg cactattcgc tgccgtttct
    13021 attgctagtt gtagcctttc taggtggtgt tttagctgct tgctgtgtct atcttgcggc
    13081 ctatgatcga aatggctttt tgggtatgaa tcgaatctta ttgtccggta ttgccgttaa
    13141 tgccggcctt tctgcattaa cattgctatg cacgatccaa ttatctaagg aaaattatgg
    13201 atttgtgaat gcatggttag cgggatcaat ctggggtgcg agctggcctt atgtttgggc
    13261 attgcttcca tgggcggtta tcttaattcc gctaggagga ctttatgcac agaggattgt
    13321 attgctatca tttggtcaag aacgcgcgca aggattaggc cttaacgtgg ctaaaagtca
    13381 aaaaatcctt ttgttgttag ctgttgcttt ggcaagcggt agtgtagcgg tgacaggtag
    13441 tttatctttt gtaggactac tagctcctca tgctgcgaag ctagtcgtac gtaaagaaaa
    13501 taactggacc tttgttctga gcggtttgtt cggttccatt tttctgctta gtgcagatat
    13561 ggttgctaga gtgattctac taagtgggga agtacctaca ggaattttga ttgccctttt
    13621 tggtgcgcca tattttctgt atgcactttt tatcagacaa aaacacgcgc tgaacagttg
    13681 aaattcatta aaaaaggaag gccaatcatt tctaggtctt ctttttattc tatttatgca
    13741 tttatttgaa agttcgattg ctatttttct ttttttccta taccatttta tgagagaagg
    13801 aagggtgagt ttttggacca attgaaagta agattacgcc ggaaaggaat cgaaaagctg
    13861 aaaaatctgg ccaaaagtgg caagaaaaag gaaaaagaac agcgaatcta taaacagcta
    13921 ttcgccagcg agtactggaa gtcagcaaaa gtgataggga cgactttatc gaatgaattt
    13981 gaactggata cacagccggt tatccaacga gcagaaaaag aacaaaagaa aatagttgta
    14041 cctaaaactt tgccgaaacg tcagatggcg ttttatgaaa tggatgaaca gtccgttctt
    14101 gtacgttcga gttttggtgt tcttgagcca caatcagaga agctctattt gccggagatg
    14161 atcgatctcc tcattgtacc aggtattatc tacaatagag ccggttatcg aatcggtttt
    14221 ggcggtggtt attacgaccg gtatttaaat aattttcata aaaaaacatg tagccttgtt
    14281 tttcgtgaac aattgaatga acaatggcaa ccggagagct ttgatcagaa gatccaacaa
    14341 ttgtttattg actaaaatga aatgggaggt tttcatgaat tatcaacagc aaataaaatt
    14401 gcgtgcatgg ttaagacgac cgttatggac ctatgctttt ttagggatac agacagcggt
    14461 cttcctcatg atggaattat ttccgcgact tgagatccct tattatgccg gaatgtatgg
    14521 tccttatctt gttcacttca atgaatggtg gcgtttagtg acaccaatct tcatccattt
    14581 tggactgatg catttcgtaa tgaactcact gatactgtat tttatggggc agcaaatcga
    14641 ggcaatctat ggccattggc gttttttctt gatttatatg ttcagcggga taatgggaaa
    14701 tacagcaagc tttgctttta acgaagcgaa tgttctgtct ggtggtgcaa gtacttcgat
    14761 attcggtttg ttcggtgcgt tgtttatctt agggtttcat tttaaacata acccagcgat
    14821 ccagcagcta gtcagacatt ttcttttatt tattgtgttg acatttgttt ttggtcttct
    14881 ggatacctct gtagatgttt ggggacatgt cggtgggcta attggcggat tagtgttagg
    14941 gaatgttttg ggtctaccga aacaacagac aagctattcg atacaccaaa ggattctttc
    15001 tacactagtt tttgtatttc tttttgtaat atgtatttta ctgggtttaa aaaaatatgg
    15061 tttacttgta taatatgaag gaagtgctta aagatggaga gcttatatga tgtacagcag
    15121 ctgttcaaaa aatttggcat ctttatctat gtgggtgcac gcatctatga cattgaatta
    15181 atgactatcg aattgcgtaa tctgtatgat agccgtttga ttgatcatga tacatacatg
    15241 acagcgtggc gcatattaaa aagagagcac cgaattgaag aaagccgaaa gaaaggaaat
    15301 ttgtaatgga caagaagatt attgggattg atttaggtgg aacaacagca aaatttgcaa
    15361 ttttaacacc agaaggagag attcagcaaa aatggagcat cgataccaat attttggatg
    15421 atggtaagca tatcgttcct gaaatcattg aatcaatcaa tcatcgtttg aatttatata
    15481 atatgaaagc cgaagacttt atcggaatcg gcatgggtac cccaggaagc gtggacagcg
    15541 aagctggaac ggttattggt gcgtataact taaactggac agaagttcaa tttgtgaaaa
    15601 aactgatcga ggcaggaaca ggaatcaaat tcgctatcga taatgacgcg aatgttgctg
    15661 ctttaggtga acgatggaaa ggtgctggag aaaacgatcc agacgttgta ttcgttactc
    15721 taggaacagg tgttggcgga ggtatcgttg ctggcggaaa tttgattcac ggcgtgagcg
    15781 gagctggagg agaaatcgga catatcactg tggatcctga agggttcgaa tgtacttgcg
    15841 gtaaacgcgg atgtttagaa actgtatcta gcgctacagg tgtggtacgc ctaggtcgtt
    15901 tcttggcaga agaatacgca ggggattcaa aactgaaagc aatgctagac aacggagaag
    15961 aagtaacaag taaagatatc tttgaaatgg ctcaagaaga cgatccgttt gcattgatgg
    16021 ttgttgatcg cgtatgcttc tacttaggtt tagcttgcgg aaacttgggg aatacattaa
    16081 atccgtcaag tatcgtgtta ggtggaggag tatcggcagc aggagaattt ttacgtagcc
    16141 gtgtagagaa atattttaaa gaatttactt tcccgcaagt aagagaatca acaaaaatca
    16201 aattagcaga gcttggtaat gaagctggtg tcattggtgc tgcctcatta gccttgaaat
    16261 ttgcttaaaa aaacggaggc attacatggt tttatgggta attaatatca ttttgctatt
    16321 gattgtgctt gcaatcatcc tttgggaagt ctatctacga gtaatggcca aacgttcagc
    16381 aacgactttg acagaagaag aatttcgaga aggaatgaga aaagcacaag taatcgatgt
    16441 tcgcgaaaaa gatgtgtttg atgctgggca tattttaggt gcaagaaaca tcccttacac
    16501 tgttttgaaa gattcattag gatctattcg aaaagaccag cctgtctata tttacgatca
    16561 aaagaaaagt ttgagcatcc gtacagcaaa taaattacgt aaagcaggtt atcaagagat
    16621 ttatttactt aagggtggat atgacggatg gtctggaaaa atcaaaagta aaaaagtata
    16681 aaacagatga ttgtatgaca gctttaaaaa ccgaatcaat cattagattt tgaccaagat
    16741 cacatgaacg gcgcaatgga tagttgtgcc gttcattttt tttattttgt gctattatct
    16801 acatatataa gctaagcgaa aaggtagaaa gaaggaaaca actatgatca aattaaaacg
    16861 tgtttacctt gatgctgaaa aagaggatgg ctatagaata ctcgtggata gattctggcc
    16921 acgaggaatt tcaaaagaaa aagcgcagtt agatctgtgg ttgaaagaaa ttgcacctag
    16981 taatgaagca cgcaaaactt ttgaccatgt accagaacga tttgcttcat ttgcaaagaa
    17041 ataccagcaa gaattgattt tcgaagaaga aaagaagcaa gctattttta aactcagtca
    17101 acttatgaaa gaacatccag tcattacttt tgtttatggt gcaaaaaatg aacatcagaa
    17161 ccatgctgtt ttattgaaaa aatttatcga aatggaggca tgcaaatgaa aatagggatc
    17221 attggtgccg gacctagagg attaagtgta gcagaaagga ttctccataa taatcaggac
    17281 agacattctc ttgaactttt tcttttcgat cccaatggtc ctggaggtcg agtttggcgg
    17341 ttgaatcagc caccggagtt attaatgaat tctgtttctc aacaagtgac gctttttaca
    17401 gatgaaacat tgtccagtgg aggattggta acacagggac ccaacttgtt tcaatggagt
    17461 aaaggacctg ctacagatta tatcgaacag acggtaacca aaaataagca actaattata
    17521 gaagaaatca atcgtctgga aaaagatcag caatctactc gatgtttata cggactctat
    17581 caacgctggt ttttctcaaa gttgcaagaa gaatttccag cttctattca gtttgtttat
    17641 tctttagtaa aaaaagtaag caaaagaaaa gatggttatc ttttacagac agatcagcaa
    17701 agctattcta tcgatcagct tgttttagca agcggtcact gggaaaatga tgagacagaa
    17761 gaagaaagag tgtgggtaga ctacgcaaag aaaaatgttt tactgtatca gcttccttct
    17821 aatcctgcag atgtagatct ttcgaagata ccgcctaaag aaaatgtctt tttaagaggt
    17881 ctaggcttaa gtttttttga ttatgtcgga ttgtttacag tatcaagagg agggcgcttc
    17941 gagaacgaaa agggaaaact ggtctatcat ccatcaggca aggaaccgat tgtatacagt
    18001 ggatcaagaa gggggctccc ttattaccct cgaggaagaa atcaaaagca aggcggagca
    18061 atggcatggc cacggttact aacaaatgaa aacctggagc aatggcatca gtcgggattt
    18121 ttgactggcg aaacattttt tgactatctg aaaaaagacg ttgagttgtt ttattacaag
    18181 aaaatcatcg aagaatatca cttgccgatt ccaaagaaaa attttgagca tgattttctc
    18241 tccctctctt cagaggagtg tctcggaaag catccagcac taatccctta tagatgggat
    18301 tggtcgtatt tagaaactcc tcttgcctat cagaatgagc ccttttcaga agctagccga
    18361 atatttattg aagggcagat aaaagaagcg gaaaaaggca attgcacagg agcatttgct
    18421 tcaacatttg atgcattaaa agactggcgt gatccaatcc ggcaggctat tgaatggcac
    18481 gttttttctg ctaaggaata caaagaaata ctatggggat ggtttactca tctaaatagc
    18541 tttttaacga tcggtccgcc aatcattcgg acgaaagaat tagcagcact gatagatgcg
    18601 ggtatttttc atttgatcga accgccattt gatattcagc aaaagcacaa ccgctatgtt
    18661 atttctctac aagataaaga aatcagcagt cgctatttga ttgaagctcg attgccacaa
    18721 acaagctttt ctcgttcaag taatcctgtc ataaaaaatt tgaaggaaaa caaacttgtt
    18781 cgcccttttt cattttcaga taatacagga agttatcatt cagaagctat agatgttgag
    18841 cgaggtacga atcgcgtgat tgatgcttca ggggaaatcc agtctgactt ttattgctat
    18901 ggaatacctg ttgaaggtgt ggattggcta actgcagcag tagctagacc ttatacagat
    18961 ccatggaatc tgagacaggc agataaaata gcacaactta tattgactga caaataaaaa
    19021 tacgcatgat gacttatctg ttttttagaa agtcttcatg cgtattttta aataattatt
    19081 aacgagaaac gcgtttacgc attgcttttt tacggttttc ttcgatcatt gcttcttttt
    19141 cgtcaattgg atcgatcact gtttttttca cagaagcaac caaaccagca acggcagcgg
    19201 ctgttgccag acttcctact aatattccag atacaaattt tttcattgaa tccacctcat
    19261 ttattatttg ttatatctat tatggaacaa aagagaaaaa aaggctagtt ttacgtttgt
    19321 ttgtaaacct ttgcaaaaaa tccttgaaaa tgtttactaa atgttttatt ataaatttac
    19381 catatgaatt tacgaaattt tactttgttt gttttcacca taaaagtaaa cttcgttttt
    19441 taaattatat agaatcaaca aacacaattt tactaatcaa aggagtgttt tttatgacaa
    19501 ttttagtact aggcggagcc ggttatatcg gttcgcacgc tgtagaccag ctagttcaaa
    19561 aggggtatca agtagctgtg gtagataatt tgctcacagg tcacaaacaa gcagtacatc
    19621 cagatgctca tttttatgaa ggagacatcc gggataaaga atttctacgt tccgtatttg
    19681 aaaaggaacc gattgaagga gtaatccatt ttgctgcaag ctctttagta ggagaatctg
    19741 tcgaaaaacc attgatgtat ttcaacaaca acgtatacgg tatgcaaatc ttacttgaag
    19801 tcatgcacga gttcaacgtg aataaaatcg tattctcttc tactgcagca acttatggag
    19861 agccgaaaga gtctccaatc acagaagata caccagctaa tcctaaaaat ccgtatggtg
    19921 aaagtaaatt gatgatggaa aaaatgatga agtggtgtga ccaagcatat ggcatgcgct
    19981 atgtcgcttt gcgctacttc aatgtagccg gagcaaaagc agatgcatct atcggagaag
    20041 atcatacacc agaaacgcat cttgtcccaa tcattttaca agtagctctt ggtcaaagaa
    20101 aagccttggc tgtttatgga gacgattatg atacaccaga tggaacatgt atcagagatt
    20161 atgttcaagt agaagatttg attgctgcac atattttagc tttggaatac ttgaaagaag
    20221 gaaatgaaag caacttcttc aatttaggca gcagcaaagg atactcagtc aaggaaatgc
    20281 tagaagcagc tagagaagtg acaggaaaag aaattccagc agaaatagca ccaagaagag
    20341 ctggagatcc tagccgatta gtcgcttcaa gtgaaaaagc gagagagatt ctgggctgga
    20401 aacctgaata cacagatatc aaagcgatta tcaaaacagc atgggattgg catgtcagcc
    20461 atccaaatgg ttatgaagaa taggggaatg agatatgtca atcagtcaaa cgatttgtga
    20521 ttttgccaca cttgcgatcg aagctggcgg atggatggaa atggaccgct tatatttaca
    20581 gaatcgtctt ttagggatgg taggagaaga tgctctagaa agcattgaag taagaccagt
    20641 taaggaagaa tctgtagact tattggatca gcttgtcgct aaggcaagag aaaatggtgt
    20701 tctttcagaa ttgccagcag aacaagagat ttttgaagcg caattaatgg attttctcac
    20761 accgccacct tcagtcgtca atgcattctt tgcacagcac tattcgaaaa gcccagagga
    20821 agcaactgag tatttcttta atttaagtaa gaaaaatgac tacatcaaaa caagagccat
    20881 cgcaaaaaac atcaaatttc cggcagagtc ggagtatggt gaactggaaa tcacaatcaa
    20941 tttatctaag ccagaaaaag atccaaaaca aatcgcagca gaacgcgagg cggtaaaagt
    21001 agactatcct acctgcatgc tttgcatgga aaacgaagga tatagaggtc gcttgaacta
    21061 tccagcccgc accaatcacc ggattattcg aatgaatcta gacggtgaaa gctggggatt
    21121 ccaatattcc ccttatgctt attacaatga acattcgatc attttatcag aacaacaccg
    21181 cccgatgaaa atcacaaagg aaacatttgc tagactgctt cggattactg aagtcttgcc
    21241 tcattacttt gttggttcga atgctgactt accaattgtt ggaggatcga ttctttctca
    21301 tgatcattat caggcaggac gtcatgaatt tcctatggca aaagcaccga tagaaaaact
    21361 tatcaaacta gaagattatc cgcaagtcat cgcaggagtc gtgaaatggc cgatgtctgt
    21421 gatccgtttg caatcaacag agaaaaactt gcttattgaa gcagctaact cagtattgga
    21481 aaaatggaaa gcatattcgg atgaaacagt atctatcaga gctttttcag cagatggtac
    21541 accacaccat acgattacgc caattgcacg aagaagggac aattactttg agttggacct
    21601 tgtccttcga gacaacaatg tttcagaaga acatcctgat gggatttttc atccacataa
    21661 agatgtccaa catatcaaaa aggagaacat tgggttgatc gaagtaatgg ggcttgctgt
    21721 tttaccacct cgtttgaaac cagaactagt agaagtagag catttcttat taggagaaga
    21781 aaaccagatg gctgaatatc atcgtccgtg ggcagaacaa ttaaaaaaag aacatcctga
    21841 tttgacgaaa gatacggtca ccgatgtcgt acaaaaatct gtaggtcagg tttttgcacg
    21901 tgtactggaa gacgctggag tattcaagcg agatgaaaaa ggacaggcag cgtttgaacg
    21961 atttactgcg attttataaa cgtaaaaaag gaggagagac aaatggcaac aattaaagac
    22021 attgcgcagt tggcaggagt ttctcctgct actgtttcaa gagttctcaa ctatgatcct
    22081 gatctttccg ttggcttagc cacaaagcaa aaaatttttg aagcagcaga acgcttaaat
    22141 tatacgaaac atcgtaaaaa tgcagcaaat actaagcaaa cgcttcgcct gatccagtgg
    22201 tacgacgata cggaagagct tgaagatctt tattatttat ctattcgcct aggaatcgaa
    22261 aaaaaggcag aagaagcaaa cgttacttta ttgaaagaaa cgtgggattc cattagtgat
    22321 cttccggcag atggaacgat cgcactagga aaattcgacg aagctcagtt attagaaatg
    22381 aaaggatccc aaaatgcctt gcttttggta gattctgacg gcacttctca aggaatcgac
    22441 tctctagttg tcgactttaa gtcaagtgtt caaaaagtca ttgaacactt tgtctcccat
    22501 aaagcgcaac atatcgctat gcttgctgga acagaatata cgaagaaaaa ccatcagcgg
    22561 ctaaaagatc ccagaattat ggcatttcaa caagttttgg cagaaaaagg ctacgacgaa
    22621 ggtccaatta tcgaagcaga tttttctgtc gagtcaggat atcaagcagt gaagcagtat
    22681 ttaaaaacga atccgcatgt tcctgatgcg ctttttgcag ctaatgatgc attagcgatt
    22741 ggcgcactga aagcgattca agaaaaaggt ttagtggtac cagaagatat ttctgtgatg
    22801 ggcttcaatg atgtcagtgt agcgaaatat gtaacgccct cgctttctac tgtaaaagtt
    22861 tataccgaat ggatgggaga gctagcagtg gagacgattc ttcaattaag taattccaaa
    22921 gcaccggttt caagaaagat tattgtggaa acagagttga ttattcgaga atcaacgaaa
    22981 taaaagatgg aaaagctgtg ggaaaatgga gtgataccgc agcttttttg tgtttcagga
    23041 aggggcaaaa tttgcccgcc agcagataat catgtattat tgtactagtt ttgcctttgg
    23101 tattttcatc ctcagaataa aaaagtatgc tacaatggaa ggcgagaatg tcatagcaga
    23161 aaggatcaaa tatatgatta cattaggaat agatactgca aaccaaactt tggcgatcgg
    23221 tgtggtagaa gacgaacaaa ttcttggaca aatacaaaca aatatcaaac gcaaccacag
    23281 tgtaacactg atgcctgcaa tcgaccaatt gtttgcagat cttcaaataa gtccaaaaga
    23341 cattgaccga atcgcggttt ctgatggtcc cggttcttat acagggcttc gtataggtgt
    23401 aacgacagca aaaacgattg cttatacatt agataaagag ctagtaggag tatctagtct
    23461 gaaaaccatc gcggctaatt gcatcggtgt ggataatatt attgtgccca tgtttgatgc
    23521 tcgaagaaaa aatgtatacg ctggggcata ccgctggaaa gaaaatagat tagaaactgt
    23581 acttgaagat gtacatattt ctgcttctga tttgttcaca caactacaaa agctgaatga
    23641 agctatttat ttcgtcggga gtgattgtca caaattcttc tctgaaataa aggaaatatt
    23701 gcctagtgcg atgattaata cagttccagc atgggatatt ccaagtggtg taactgtggc
    23761 tcaattaggt gctttagttg aaccagttgc agagaaacaa gcttttcttc ctcgctattt
    23821 aaaacgagta gaagcagaag aaaaatggtt ggagacgcac acacctggag acgaaagtta
    23881 tgttgaaaaa atttagagcg tatgccagaa gcttttttca agataaaatg atttatacag
    23941 aaaaaacagt cgaacaaatc gatcgtcgtt acgtactaag agaattgact acagaagata
    24001 tcaaagagct gctggatgtc gaacgagaag tctataatgg cgaattgcca tggacaaaaa
    24061 gtgcctttat atcagaattg aagtcaccgc ttcctcatct atatctaggt atctttgata
    24121 atggaagaat gattggtttt atcggttcaa gagcgttcgg tttagattgc catatcacga
    24181 atattgccgt tcgaccggca tatcagcgta aacgaatcgg ttcgctgctt attgacgaaa
    24241 tcgagaactt tgctataatg aatcgctgtg agacgatgtc attagaagtg cgtatgagta
    24301 atcaagatgc acaaagactt tatcggaaat tggggtttca agcaacaacg atcaaaaaaa
    24361 gatattacac tgaaacaaaa gaagatgcgt tggatatgat aaaactttta gaaaatgagt
    24421 gagattagtt gatttgcaga aaaaaagatt ttgatgaagc gtacttggca gaatgcgtat
    24481 ggatgattag cgaaaatgct tatccacatg gttcaccttg gacgaaaatg cagtttttgg
    24541 cagacatcca acagcctcac tctgattatc tgcttttagc tgaagataac cagatctgtg
    24601 gctttattgg atactcgaaa gtaatagatg aagtcgagat cacaaatatt gctgttgcag
    24661 tttcagaaca aggaaaaggg catgcacggc atttgttaca attactgatt acggaacaaa
    24721 taaaagattc gctgcatgtt tttttagaag tacgtctatc taatgaagca gcaagaaaac
    24781 tttatgaatc agaaaagttt cgtatattag gtaaacggaa atcatattat cgcaatccga
    24841 cggaagatgc gatcattatg agcagaaagt taaaaacgga gacgatgaaa tgaatgaaga
    24901 attgattctg gcgattgaat caagttgtga tgaaacgagt gtagcggtcg ttagaaatgg
    24961 aacagagatc ttatctaaca tcgttgcttc tcaaataaac agtcataaac gttttggtgg
    25021 cgtagtacct gaagtagcta gccgccatca cgtagaacaa atcaccttat gcttggaaga
    25081 tgcactggta gaagctggtg tgtcggcaga agatttatcg gcagtcgctg tcacttatgg
    25141 accaggactt gtcggctcgt tgctgattgg tatttctgca gctaaggctt ttgcttgggc
    25201 acaccaattg ccgcttattc ctgtaaacca tatggcaggg catatctatg cagcccgtct
    25261 ggttaaacca ttccagtttc cattgatggc acttcttgtt agcggaggac atacagaatt
    25321 agtctatatg caagaagatg gatcatatga aatcattggg gagacaagag atgacgcagc
    25381 aggagaagct tatgacaaag ttggaagagt attaggtctt tcttacccaa gcggaaaaga
    25441 aatcgatcag ctagctcatc agggaaaaga taattaccat tttccgcgag cgatgatcca
    25501 tgaagataat tatgatttca gttttagcgg attaaaaagt gcatttatca atttggtgca
    25561 taatgcacaa cagcgtggag aagatctaga caaaaatgat ttagcagcta gctttcaagc
    25621 aagtgtgatc gatgtattga ttactaaaac acttcgagct tgtcaaaatt atccagtcaa
    25681 gcaactgatt atagctggag gtgtagcggc caatcaaggg ttgcgtgaag gattgcagga
    25741 tgctttggca actaaactcc ctgaggtaga actagtgatt ccccctcttc gactttgtgg
    25801 agataatgct gcaatgattg gagcagcagc acatgtggaa atgcaaaaaa gaaattttgc
    25861 tagctatcag ttgaatgctg atccaagctt ggttttatct gaataaagag gttgtgaaaa
    25921 agctgccctg catcaagtaa taagaacagc ccaggaaaaa cggcttctca tgtttttgac
    25981 ctggctgttc ttattaccgc gtatgcaagc ttttgaacgc cgtttaatca agagttcgta
    26041 agcctgtcgt ttaactctga ttcacaagga acaatttcaa aaaacagctc ctcatgttta
    26101 tgaacattat gacttgtgac cgaagagttg acaggtacac accgtttaat cgaaataggg
    26161 ctgtgacacg tttttgtcaa agccctattt tttacatact tttaatcatt attgttattt
    26221 tgctattttt tacagcttat ttatcattat ccgttcctct gattgccctc actgagtaat
    26281 cgtttattgt cgaggaatat cgaataatct agatcggatg aggacaagac gcttcatcta
    26341 gttaatctaa cagtattttt tgcaaacagt gaatattaca taactgaaac acacatagaa
    26401 aatctttgag tcttgtccag tgatctaaaa ctgccggatc tttcagaagt tttagggagg
    26461 aaaataagca gtgaccctga tatttatcag ggtcactgct tatttttgtt cgcgttattc
    26521 tctcatagct ggagtagtag ctgtcttaca gaaagtaaaa cgctttttgt cataatctaa
    26581 cagcccggaa tcacgtagtt gtttgattac ttgtccagca gtttcacgtg tcgtgccgct
    26641 attcactgct aattctttta aggtgatcgg ataaggaatg gtgatatgac catcttttgt
    26701 tggtacgccc aattcttctt tcaaaatgaa taaattttgt tttacccggt caaaggcgct
    26761 agaggtcaat ccgatttgga tacgcaattc atgtatttca actactttag aaattttttg
    26821 tatcatatag atcaactgtt cgggattttt tcgtgctaat ttttcatagt aggaaacagg
    26881 aaggtagaat gtttcaatat cagtcatcgc ttgagctgta taagtgtagg tctcttctcc
    26941 aaacattcct gacataggaa aaaggttatt tgtattcaca tagtcatagt agtaataagt
    27001 tgcactttca tcataacgtt ctaaacgaac gagtcctttg gtcaagatat acaaccgttg
    27061 tctgggatca ccatcgtcga aaaggatctg tcctttttta taagaacgaa gaacagtatg
    27121 ttccttgatc tgttggaact cttcatccga gaaatgttca aaatcaggtt gatatctgag
    27181 ttttgaaaag tgaaaatcga ttttattaac acgcattaaa aagtcctctc cttattgttc
    27241 actatattta ttgtcacttt ttttaaagaa tttgtaaagt tttaacatac caacattaga
    27301 aacaagtttt aatactttac aaatataatt tgttattatg taaaaaatat acaatggtat
    27361 tgaataattt gttttgaagc gaatgatttg tgta
//
LOCUS       DACDUU010000036        27203 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.036, whole genome shotgun sequence.
ACCESSION   DACDUU010000036 DACDUU010000000
VERSION     DACDUU010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.036"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..99
                     /locus_tag="IWL30_001535"
     CDS             <1..99
                     /locus_tag="IWL30_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9706300.1"
                     /translation="IKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
     gene            227..1441
                     /locus_tag="IWL30_001536"
     CDS             227..1441
                     /locus_tag="IWL30_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9706301.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            complement(2151..2339)
                     /locus_tag="IWL30_001537"
     CDS             complement(2151..2339)
                     /locus_tag="IWL30_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706302.1"
                     /translation="MAVREYHYLTVQVMAIKEDKSFTRTQIGFRSLLQGKGRGVFVST
                     ASCLSNVLNSFIQLSKND"
     gene            2430..2639
                     /locus_tag="IWL30_001538"
     CDS             2430..2639
                     /locus_tag="IWL30_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HAP9706303.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            2751..4364
                     /locus_tag="IWL30_001539"
     CDS             2751..4364
                     /locus_tag="IWL30_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4368 domain-containing protein"
                     /protein_id="HAP9706304.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            4399..5610
                     /locus_tag="IWL30_001540"
                     /pseudo
     CDS             4399..5610
                     /locus_tag="IWL30_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            5633..6559
                     /gene="glsA"
                     /locus_tag="IWL30_001541"
     CDS             5633..6559
                     /gene="glsA"
                     /locus_tag="IWL30_001541"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAP9706305.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            6767..8227
                     /locus_tag="IWL30_001542"
     CDS             6767..8227
                     /locus_tag="IWL30_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706306.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            8448..10211
                     /locus_tag="IWL30_001543"
     CDS             8448..10211
                     /locus_tag="IWL30_001543"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAP9706307.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            10295..10753
                     /locus_tag="IWL30_001544"
     CDS             10295..10753
                     /locus_tag="IWL30_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP9706308.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            10862..11545
                     /locus_tag="IWL30_001545"
     CDS             10862..11545
                     /locus_tag="IWL30_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP9706309.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            11692..12306
                     /locus_tag="IWL30_001546"
     CDS             11692..12306
                     /locus_tag="IWL30_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAP9706310.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            12322..12528
                     /locus_tag="IWL30_001547"
     CDS             12322..12528
                     /locus_tag="IWL30_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706311.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            13069..14199
                     /locus_tag="IWL30_001548"
     CDS             13069..14199
                     /locus_tag="IWL30_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP9706312.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            14291..15046
                     /locus_tag="IWL30_001549"
     CDS             14291..15046
                     /locus_tag="IWL30_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP9706313.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            15104..17143
                     /locus_tag="IWL30_001550"
     CDS             15104..17143
                     /locus_tag="IWL30_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAP9706314.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(17252..18403)
                     /locus_tag="IWL30_001551"
     CDS             complement(17252..18403)
                     /locus_tag="IWL30_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP9706315.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(18557..19087)
                     /locus_tag="IWL30_001552"
     CDS             complement(18557..19087)
                     /locus_tag="IWL30_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706316.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(19186..19536)
                     /locus_tag="IWL30_001553"
     CDS             complement(19186..19536)
                     /locus_tag="IWL30_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAP9706317.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(19693..21222)
                     /gene="ahpF"
                     /locus_tag="IWL30_001554"
     CDS             complement(19693..21222)
                     /gene="ahpF"
                     /locus_tag="IWL30_001554"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAP9706318.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(21232..21795)
                     /gene="ahpC"
                     /locus_tag="IWL30_001555"
     CDS             complement(21232..21795)
                     /gene="ahpC"
                     /locus_tag="IWL30_001555"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAP9706319.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(21939..22601)
                     /locus_tag="IWL30_001556"
     CDS             complement(21939..22601)
                     /locus_tag="IWL30_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9706320.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            22785..24431
                     /locus_tag="IWL30_001557"
     CDS             22785..24431
                     /locus_tag="IWL30_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP9706321.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            24422..25504
                     /gene="uxuA"
                     /locus_tag="IWL30_001558"
     CDS             24422..25504
                     /gene="uxuA"
                     /locus_tag="IWL30_001558"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP9706322.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(25612..27165)
                     /locus_tag="IWL30_001559"
                     /pseudo
     CDS             complement(25612..27165)
                     /locus_tag="IWL30_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
BASE COUNT         9179 a         4669 c         5388 g         7967 t
ORIGIN      
        1 ataaaagata ttatcctaca ctttgaaaaa tacgattatc gttttcattg gaatagtgat
       61 aattcagtta ttgaaatttg gatacctatt caagattaga agcaatttta tatccataca
      121 cacaaagcag ttagtcttag gattgactgc tttttctata cagattttta atgacaggca
      181 attatcaatt atggtagttg ccttttttga ttggaggaat tgaagaatga atgataaaaa
      241 ctttattgaa gaactaagac aaaagcgtga ggaatacgga gtaacacaaa caaggcttgc
      301 tgttgcctgt ggtatcagcc gtgaatatta caaccgcatt gaaaaaggaa aacagccatt
      361 gaatgacgaa ttaagagaaa tcatagaaaa acagattgag cgtttcaatc cgcaagaacc
      421 actattctta ttgattgatt actttcgtgt ccgctttcct actacggacg cattggcgat
      481 tatccgtgat gtattacaac tgaaatctaa ttacatgctt tatgaagatt atggaaaata
      541 cggatatgaa agcaaatatg tacttggcga catcaatatc atgtgttcca tgcaggagca
      601 tttaggtgtt ttattggaac tgaaaggcaa aggctgtcgg caaatggaat gttatctgct
      661 ggcacaggaa cgctcatggt atgacttcat gctggattgt atgacggctg gtggtgtgat
      721 gaaacggctt gatttggcta tcaatgataa agcaggaata ttggatattc caaagttaaa
      781 ggaaaaatac aaggctggcg aatgtatgtc ctacttccgt atgcagaaag attacagcgg
      841 tacagaaaaa tgcggtagcg atttaccaaa gaatacagga gaaaccttat atctcggttc
      901 aacaagtagc gaattatata tgtgtgctta tcagaaaaat tatgagcagt atgtcaagaa
      961 tggcacagaa attgaagata cggagattaa gaaccgtttt gaaatacgca tgaagaacga
     1021 acgagcctat tatgcggttg tagatttact gacctatcgg gacgctgaac gcaccgcttt
     1081 ttctatcatc aatcattatg tccgctttgt tgacagagag gacgacaagc caaaaagtca
     1141 atggataaca aatgatgatt gggcatggtt tgtaggggaa aacagagagc cgatacgctt
     1201 aaccacaaag ccagagcctt acactttaca aaaagcgttg cattggctac aaagacaagt
     1261 cgcaccaacc ataaaaatgg tacaggcatt agacagagaa aatcgtacaa cgatactgaa
     1321 agatatgatt gagcaggcag aactaaaaga caagcataaa catttattac aattagagaa
     1381 atcaaccata gaagaacgta tagataccgc tgttccacaa gagaatgacg gtatttttta
     1441 atgtaattga aaaatttcag atttagtcag caaagagctg ttttcattcc ctatgtgaag
     1501 tgaaagaaga aaaataaatt ttttattaaa tctgcaacaa aatggctact tgcgtacaga
     1561 tatggtgcga gaaaaggaaa tctaaacttt ttttgaaaat aggtcattaa atcggggctt
     1621 gctgtcccta tatgaagtga aagaagaaaa aagttttgaa aaaatgacgg aaattcgtga
     1681 ttagtggaca gtcttctgtg aaaagaggaa aaacatcatc attttgtatt caaacaggca
     1741 ttagcagttg agtgttgatg tgaaaagatg aaaatacttt gttctcaact taacaattta
     1801 aggtttccgt tccctatatg gtgcaaagaa aatatttcat tctattgttg gcagtaacgg
     1861 tatggtttgg gtagttaggg tgtgaaaagg aaaatattct tttttcatca tcaacttagc
     1921 aaaatgacat tttctatccc tatatggtgc atgaaaagaa gttaaaattt ttccatttaa
     1981 acttagcaaa tggacgtttg ctgtacagat agtagtgaaa aaggagaaaa tcaaccgttg
     2041 attgccaaaa tgaaccgttc catttgtact aataatggaa aaagttttgg attttggtta
     2101 cgtttctcct cttttccaac gcgatatata ggaaagacga tttttttctt tcaatcgttc
     2161 tttgacaact gaataaagct attcaatacg tttgacagac agctagccgt tgaaacaaat
     2221 acgccacgac ctttcccctg caagagcgag cgaaaaccta tttgtgttct tgtaaaagat
     2281 ttgtcctctt ttatcgccat gacctgtact gtcagataat gatactcccg tacagccaca
     2341 gcttgagcat taagagcgtc gcacgcaatg ggtacggcta cataagaact atgcagaggt
     2401 ggaactcctg tgggctatga caaaagccgt tgaattgctt agaaagattt tacagaaagg
     2461 gggtatgtgt tattgataat gctgtaaaaa caattcaaaa gaaaaatggc aagtgtggca
     2521 ttggcaacag aggaaagaca aagattgtag taaaagagca tttttccgaa aagggcaaaa
     2581 caatggaaga acttctaact gatgtcatgc tggaaaaagc aaagcagaca atcgcataaa
     2641 atcggtgcag ttgtaagaaa tagattgaat gacaaaaagt acccgtgtta tactttactt
     2701 gcaagcaggt attgtaaaca cgggttagtt ataaaggagg ataactgaca atgaaacaac
     2761 agatttacaa tactgcactt tatcttaggt taagccgaga tgatgaatta cagggcgaaa
     2821 gttccagcat taccacacag agaagtatgt tgcgtttata tgcaaaagaa catcatttga
     2881 atgtcattga tgaatatatt gatgacggct ggtcgggaac aaattttgac agaccgagtt
     2941 ttcaaagaat gattgaggat atagaggcag gaaaaatcaa ctgtgttgta acgaaagacc
     3001 tttcccgtct tggcagaaat tatattatga caggacaata tacagaattg tatttcccta
     3061 gtcataatgt ccgctacata gcgattgatg acggtgtaga cagcgaaaaa ggcgaaagtg
     3121 agattgcacc atttaagaac atcatcaatg aatgggtggc aagagataca agccgtaaag
     3181 tcaaatcagc atttaagaca aagtttgcgg agggggctca ttacggtgct tatgctccgt
     3241 taggatataa gaaacaccct gacatcaaag gaaaactgtt ggttgatgat gaaacaaaat
     3301 ggattattga aaaaatcttt tccctagcct atcaaggtta cggaagtgca aaaatcacaa
     3361 agcagttaag agcagaaaaa gttccgacag cgtcatggct aaattttaca agatacggta
     3421 cttttgcaca tatctttgag ggaaaacccg aaagtaagcg ttatgagtgg acgattgctc
     3481 atgtcaaggc gatattgaaa agtgaggttt atatcggaaa cagtgttcac aatatgcagt
     3541 ctacggtatc gttcaagagc aaaaagaaag tgcgtaagcc cgaaagtgaa tggtttcgag
     3601 tagaaaacac tcacgagccg attattgaca aggaagtgtt ctatcgtgtg caggagcaga
     3661 taaaatcaag acgcagacag acaaaggaaa aggcaacgcc gatttttgca gggcttgtca
     3721 agtgtgcgga ttgtggttgg tctatgagat ttggaacgaa taagacaaat aaaacgcctt
     3781 acagttatta tgcttgcagt tactacgggc agtttggcaa aggtaattgt tctatgcact
     3841 acattcgtta tgatgtgctg tatcaagccg tattggaacg cttgcagtat tgggctaagg
     3901 cagtacagca ggacgaagaa aaggtattga acaagataca gaaagctggc aatgcagaga
     3961 gaatacggga aaagaagaaa aaggcaagta cattgaagaa agccgagaac agacaaaatg
     4021 agattgaccg tttatttgcg aaaatgtatg aagatagagc ctgtgagaag ataacagagc
     4081 gaaattttgt gatgttgtcc agcaagtacc aaaaagaaca gatagaactg gaacagcaga
     4141 taacaagcct aagagaagaa ctaagtaaaa tggaacagga tatgataggt gctgaaaagt
     4201 ggattgagtt aatcaaggaa tattccgtac caaaggaact gacagcacca ttattaaatg
     4261 ccatgataga aaaaatcctc atacatgaag caacaacgaa tgaggataac gaaagaatac
     4321 aggaaattga gatatattac cgatttattg gaagagttga gtaatcaaca agagtaatat
     4381 ttttaactaa gggaaaccgg cggtgtctat acttgggtca gtcgcacatt gggaaaacga
     4441 ttcggttttg ccgcaatctt tttccaatgg tttgagataa cagttggcta cttgaccatg
     4501 ctgtatttct taactggtgc attatcctat gcaacgggta ttggtgctat acaaaacaat
     4561 aaatatctga aattggcaat cctattgatt attttctggg ctattttact ctcacagtta
     4621 aaaggaacca aatacacttc attgattgct cggatcggtt ttgtagctgg tatcttgttg
     4681 ccagcattag tcttgtttgg cttagggatc cattatgtcg cttcaggcgc tccactacaa
     4741 acgatactgt ctatgaaaac attgattcct gatttttcga aattgcctac gctggttgtt
     4801 tttgtttcct ttattttagc atatatgggc gtagaaacat ctgccagtca tgcaaatgaa
     4861 atggaaaacc ctaaaaagga ttatccatta gcaatgtttg tattggttat tctggcaatc
     4921 attttagata cttttggcgg attgaccgtc gctactacag tacctcaaca tggtttgtct
     4981 cttaatacag gggtgattca atcgttagaa ttcctattaa gacacattga tccaaatctt
     5041 gcatggattg cgaaaattct tgcgattctt gtttgtttag gtgtcatcgg agaaattgct
     5101 tcttgggtaa cctctccttc aaaagcactt cacgtagcag cagaagaagg attacttcct
     5161 gaatattttg caaaagaaaa cacacacggt gttcctgctc atttgatgat cgcaaatggg
     5221 atcgttgcaa caatctgggc agccgtcttt acattaagcg gcggggggaa taatctttca
     5281 tttcttgcag cgatgtcatt gactgttgta atctacctga tgttgtactt tatgtttttc
     5341 gcgggctatt tgaagctggt ttttaaagat gacagtcttg aaagagccta tcaggttcca
     5401 ggcggaaaag ttgtaaaagt aatcgttgct tgtttaggtc ttttgacttc actttttgct
     5461 ttttgtatct catttgttcc acctgctagt atcgcagctg gacagcatgg gatttatgaa
     5521 acgattctga ttgtttctta tcttatcgtc ttagttattc catttacgat ctacgcatgc
     5581 cgtcataaat tccagcatgc agaaaaataa aaaatgaaag ctggttattc aaatggaagt
     5641 aaaagaatta gaacaattga ttggaaaaaa tttccactat tatcaaacag gacaagtggc
     5701 ggattacatt ccagcattag ctgaagtgga tcccaaacaa ttaggtatgg cgatttatga
     5761 tttggataca aaacaactga ttgagggagg agacgccgat gtgcgctttg ccatcgaaag
     5821 catgtcaaaa gttcccgtat tgttattagc aatcaaagac aacggaatag aaaaagtctt
     5881 tcaaacaatc aacacggaac caaccggttt tgccttcaat tctatcatga atatggaaat
     5941 caatcatcgc aaacatccaa tgaatccttt tgttaatgca ggagcgattg caacaacctc
     6001 gttgattgcc ggtaaagatg cagatgaaaa atttgagcga atcttgtcat ttatgaaaga
     6061 gatctgtgat gatcctaata tcacattgaa tgaagaaatc tatcattctg aatcaagaac
     6121 aggtgatatc aatcgctctc ttgcttacta tatgaaaggt aatcaaatga tcgaaggaga
     6181 cgtaccggag atactcgatg cttatttcag acaatgctcc ctcaatgtca ccgctacagg
     6241 catagcaaaa ttagctgctg tgctggcaaa taaaggaatt gctccttgga atggcaaaca
     6301 aatcattact gaagaaagtg ctacaatcgt caaatcaatt atgacaactg ctggtttgta
     6361 tgatgaatca ggagagtttt ccgtccatgt tggtgtacct gcaaaaagcg gtgtaggcgg
     6421 cgggttgatg gcagctgtgc caaatcgtta cggcataggt gtcttcagcc cggcactaga
     6481 tccatttggt aacagcgcag ctggtatcca attgttgaaa gatgtagtca aagaactaga
     6541 cgctgacatc tttgaataac cattcttgaa agaggctggg gtaaaagtcc aaacggctca
     6601 aagctaccta caatgaggct gaaattgtga gcttgtttga cttttgtccc agtttctttt
     6661 tttattggtt tttgaaaggg cttttctaaa taaaaaggaa acaaaaatac gatttacaga
     6721 tatgattaaa taaaatcata agaaaaactg gaggcgtgta tcaggaatgc tggaaaatta
     6781 catcgagcga aatatattta gaaaagtgta tctgtgtgaa caattatttg aattccaaga
     6841 aatagacatc gaacaaacag ctatttctct aagggtgact acaccaacga tccttcacga
     6901 tctagaaagt ctagccgagt gtctagaata ttgtataaaa gaacaagtaa gagagaagca
     6961 taagtataaa ctcgtattca aacatggtat tgctctttct gagctcacac agtttctgta
     7021 cggtcaatct tattttttaa agtttttaag ttattatttt aatggtatat tcacgagtac
     7081 agaactggct gatcttgaat ttatttcttt gagtaaagta tatacaatca aaaaaatcgt
     7141 attggatttt tttaaagaaa atgattattt gaaaaataaa gaaatcatta tcccagaatt
     7201 tgactcgcgg aacatcttgc tagctttagt acgttatgtc aattgggaag gctatgaaaa
     7261 taaaaatccg tatgtagaag aagcttgtaa cgagttgatt gattatgtag agactcattt
     7321 ttttaaaaga agatattcgg aagatgaaaa gtcctttatc atacgagaaa tagagatcgc
     7381 aatcggaaga aaggcatttc cccttggact gaaagaagaa gataagaaaa gcgcagaaac
     7441 gaagccgtta tttcaattag tctgtcaagg attggaagag caaaaaggaa ttctagactt
     7501 acaagaagaa gatgtctatt atattttttc tttgttcaac agtagaaatt ataccaatga
     7561 gaacatggag ctgttaagaa aagatttcga agttgtttat aaaaattttg ttctcaatga
     7621 cctatcactg aatgaattga ttttacagat tattagtatc gctgaaatcg acgatgcaga
     7681 aaattttatt ttcaagcaat cctttttaca atttgtccgt acgttgtggg cagatggaca
     7741 agtattttta ccggaaagga tctatcttct atcagaaaaa gaacaacttc tgtacggaaa
     7801 aattgtagaa atattggaag agtggaaaaa gaaaaatcaa atccaactaa gatggaatga
     7861 aaatttcatt cgaaaattcg tcaaaagtct ttctttgatc aacagtgaac aagcggggag
     7921 acaggaaata gaaatttttg ttgtttcaga aagttcagtc aaacaatttt tttatcgaac
     7981 ccaatttcat ttgtatgcgg aagaaggcat cgctgaaata aatcctattt tatttcgttc
     8041 attagaagaa ttgcctgatg aatgtctatg tgcgaaaaaa agagtggttt tgtgcgatgc
     8101 tgctagctat caaaatggat tagaaactga aaagacaaag atctatccaa tctcattaaa
     8161 aggtgcaaac atccatttgt atcatattgc tcaggaactt cacctattaa aaaaacatat
     8221 ttcttgatga gaccttaatt aggagacctt ctacaaagct ttttaattgt ctgtagaagg
     8281 tttttatttg agtcctgatt cgtgcgaaga tctacttata taacaagctt tctctgaaat
     8341 ttttttaaaa tgctattgac ttagagtcaa ctctaagtag tagaatagat aaagtgtaac
     8401 aaatctattt tcattaaaag aagatagtac ggaggagaga atgtattatg agtacagtat
     8461 tacatgtatt ggatgcaatc aatagccccg cagatgtcaa aaaattatct gttgaggaaa
     8521 tgaaacagtt ggcagaagag atgcgccaaa aaatcttgca aaaggacagc gatgtaggtg
     8581 ggcacgttgg tccgaatcta ggtgtagtag aattaacgat tgcgtttcat tatgtatttg
     8641 attcaccaaa ggataaaatc gtttgggatg tttcccatca gtcttatggt cataagcttt
     8701 taactggtcg aaaaaatggt tttgaaaaag gacattaccg tgaagtgagc ggatattcca
     8761 accaacatga aagtaaacat gactatttta cagtggggca cacatctaca tctatcagct
     8821 tagcaacagg tttagctaaa gcgagagatc tgaagaaaga atcaggaaat gtggtagcat
     8881 ttatcggtga cggctcccta agtggtggct tagcttttga aggattgaac aatggtggtg
     8941 ttttacaatc caatctaatc gtattagtaa acgataatga gatgtctatt gatgagaatc
     9001 atggcagttt atataaaaac ttagctgagc ttcgtcaaac caacggtaaa agcagcaata
     9061 acctatttaa agcaatggga tttgattatc gttacgtgga taatggaaac gatctggaaa
     9121 cggtgatccg tttatttgaa gaagtaaaag atattgatca tccgattgta cttcacgtcc
     9181 atacggaaaa aggtcgcggt tatcagccgg caatcgataa taaaatgaaa tatcattggc
     9241 atgtaccatt tgatctgtcc accggggagg caaagcaagt aacttctgga gaaacattta
     9301 atatgatcat catggattat ttggataaga aaatgcaaac aagtacagct cctattgccg
     9361 cagtcaatgc agcgatacca ggcgcctttg gactgaaaac attccaagaa aaacatccag
     9421 atcattattt tgacgtgggg atcgctgaac agcactctat cactttcgct tctgctttgg
     9481 cttctcaggg agtacgtcca gtcgtcttcc aatcaagtac tttcttgcaa cgggcatatg
     9541 atcaattatc acatgattta gcgatcaacg agaatccagt gattatttta gtcggtggcg
     9601 gaacgatctc agctagtgac cgaacacacc aaggaacatt tgacattcct tacttatcaa
     9661 gtatcccgaa catcaaatat cttgctccag caacaaaaga ggatatgatc tctatgctag
     9721 attgggcgtt cgaacaaaca gcgcagcctg tagcaatccg aattcctaca catggtgtag
     9781 aacatggtac agctatcgca aatgattata gtattccagc ctatcaaaca gtaaataatg
     9841 gaggaaaagt agcaatttta gctttaggcg gttttcttga attaggaaaa gaaacagtaa
     9901 aagcttttag aaaaaaagga atcgaagcaa cgttgatcaa tccaggaacg atcacagaaa
     9961 atgacgtaga aacattagat aaattaaaag aaacacacga attagtcatc acattagaag
    10021 atggtagttt gtcaggagga tttggcgaaa atatcagtcg ttattatggt gattcttcaa
    10081 tgaacgtatt aaattttggt gcagagaaag aattcacgga tagcgttcct gtagggcagc
    10141 tttatgaacg ttatcattta acaccaaaac aaatcgttga agatagtctg cgtgtcttga
    10201 atattcaata attcattatg ctcaccatcc agataattgt ttatactagt atctggatgg
    10261 ttttttcatt ataaaaagca aaggggtgga tgttatgtat cagcaagtag aagaatatgt
    10321 caaaaagttc caagaaaaat atcctagtta tcaatcagaa aaattaggtt tcttttcatt
    10381 tggggataca gaaaaaatgg cagacgagtt gagcgaacta gttgtaaaaa agataaagac
    10441 tggtacatca agtggttacg atctgtataa acaagagaat gagcctttac caaaagttgg
    10501 tcaattagac gtggtcctga atggaaaaaa tgaaccagtt tgtatcatca aaaatataaa
    10561 tgtgaccatc ttgcctttta gtgaagtcac gaaagagcat gcttggaaag aaggagaagg
    10621 ggatcggaca ttagcttatt ggcggaaagt ccatgctgag ttttttaaac atgcttataa
    10681 agaagcgttg aacatagaat ttacagaaga gaagctggtc gtatacgaag aatttgaagt
    10741 aattttcttt tagtaagaaa ggtctgctga aagaaatagc tcattattgt ctcctgaata
    10801 gaaaagggtt acatttttat atgaagctgt atttttatat aacattactg gaggagatat
    10861 tgtggataca ctggaacaaa tcaaaggcgg agtcgtcgtt tcttgtcaag ctttggaaaa
    10921 cgagccgcta cacagttctt ttattatggg gagaatggct ttggctgcaa aagaaggcgg
    10981 agcagttggt attcgagcaa atacgacaga agatatcaat gaaatcaagc gaatcgttga
    11041 tttaccggta atagggatta tcaaaagaga ttacgatggg tcagaagtgt ttattactgc
    11101 gacagaaaaa gaagtggaag aacttttgga gacgcaagca gaaatcattg cactggatgc
    11161 aacttctcgt aaacgtccga cagaaaaaac aactgctgaa cttgtccaaa tgattcacga
    11221 acacggtcgg ttagctatgg cagatatttc gacttttgag gaagcgcttc aagcgcaaga
    11281 agatggtttt gatcttttgt ctacgacttt agcaggctat acagaatatt cacgaaagac
    11341 gcagacacct gattttgatt tattgaatca aatcatagaa caagtcgata tcccagtaat
    11401 tatggaagga catactgact ctcctgaaca agtagagaaa gcatatgaat taggtgcttt
    11461 ttcagtcgta gttggatcta ttatcacccg tccgcaattg atcactaaaa gatatgtcga
    11521 agccgcaagt aaagcaaata actaaaaaac acgcacggca cagattcttg acaaaaaaga
    11581 aaaattgata gatagtttaa agcagattct aagaagatga aaaaagcatt acttttcatt
    11641 ttgtcttcaa gatctgcttt ttaacgaatt ttatagaaag aaggcgaaac catgtttgaa
    11701 cggagtcctt cagaattgac agaacgtgaa aattacaagt tgctgattgg ttcagtcatc
    11761 cctagaccag tagcagtagt aacgactaaa tcagcaaaag atatagtgaa tatcgcaccc
    11821 ttcagttact tcaatatcgt cagttcgaat cgacccatct tatccttggc gatccagaga
    11881 aaacaagatg cagtaaaaga cacagcaaag aatattcttg agacaagaga agctgttatc
    11941 catatccttg atgaggacaa cgtagaagaa gtaaataaaa cagcagccaa tctgcctaat
    12001 gaggaaagcg aactgtacct gactgacttg actttaactg attcagtaat catttcagta
    12061 ccagcattga cagaagcaaa agttcgtttt gaaacatcct tatatgaaca tgtcgagata
    12121 cggaataaaa ataaagtaac agctgattta tttttgctag aagtacagaa atatcattta
    12181 aatgaaacag tctatgatga taagacagga aggatcgatc caagaaaatt gaatgctgta
    12241 agccgtttgg caggaaatga ttatgcagca ataggagaaa ttttcacgat tgcacgtccc
    12301 gaataaaaag gagcactagt tatgttaaaa ataagctata aaccatcgac agattcaaaa
    12361 gaaatgaaaa aagaatatga aacagtgaat gattttctgc aagggcagta cttagaagtt
    12421 ccgccattgc aagaccattt tgtcgttact actgttactt tagatggtaa ggaaatagaa
    12481 atgccagatc aaacgattag cggattgttt aattacttta acaaataaat atttattccg
    12541 aaaagtgaaa aaacatataa aaaaacacat aaaactaaaa aattatcaaa gaatcctggt
    12601 gcaactgaaa gaatttgcaa cgggattctt tcaacttatt tgttattttt ttctaaaaaa
    12661 tattaaagat tgttaaattt cactatttca tttcaaattc cattgcctaa tcattaggaa
    12721 aagtctatca tgaactcgta aatagaatat agtaataacc ttgttaggtg aggctcctat
    12781 acaaacatag gctgttgccc agaaacgtcg aaagtcgcca atgggtaaaa cagaaactgt
    12841 tggattaagg ctttttccaa tagctaagaa tgttttcttt acgttgtata gtgctaaaac
    12901 tcggacgatg agatgatgct aggtcagaat tcttgttcga aaaggtttct gaccttttct
    12961 ttatattttt gtgactgact tatttgatga gacaaactcg ctcctcacct gacaagtgga
    13021 ttgctgtgtg aggaggagat gcggtgaaaa aagatcgatt ggtggatttt gggtccttat
    13081 ttgactttgt ccatgatcgg tcttttagaa gtatacagtg ccagttctta tcgtttattg
    13141 caagccgatg aaaatacaaa aagtctatta ttgcgtcaac taattttcat atttttgagt
    13201 tggagcgtga tcttcttagc tcgttcagtc aaactacact atttacttca ccctaaaata
    13261 gcaggatacg gtttagcctt atcgattttc tttttagtat tagtaagaat agggatattc
    13321 ggtgtcactg tcaacggcgc acaacgttgg atctctctgt ttggcattca attccagcct
    13381 tctgaactgg caaatctttt tttgattttt tatttaagct ggttttttcg tgacggaaat
    13441 agtagcccaa aagatctaaa aaaaccattc ctgattacag taggtataac ttttctgatt
    13501 ttatttcagc caaagattgc tggagcattg atgatccttt cgattgcgtg ggtcatattt
    13561 tgggcagcgg cggttccatt taaaaaaggg atctatctaa tcgttacttt ttctgcattg
    13621 ctgattggag cagcaggcgg ggtattatat ttaggaaata aaggttggct tccacagatg
    13681 tttaatcatg cttatgaaag gatcgcgact ttaagagatt cttttatcga tagtcatggt
    13741 gcgggctatc aaatgaccca ttcgttttat gcactatata atggtggcat atttgggcgg
    13801 ggattaggaa atagtatcac aaaaaaaggg tatcttccag agacagaaac agattttatt
    13861 ttttcgatta ttacagaaga gcttgggcta atcggtgcat tgtgtgtgct tttcttattg
    13921 ttctcattat gcatgagaat cttttgttta agcagtcgct gtaaaaatca gcaagctgga
    13981 ttgtttcttt taggattcgg aactttgcta ttcgttcaga cgataatgaa cgtgggaagt
    14041 atcgcggggc tgatgccaat gactggtgtg ccattgccat ttgttagcta cggaggaaca
    14101 agttatctga tcctttcttt aggaatcgga atcacgttga atatttcttc aaagatacag
    14161 gccgaagagt tggcgctcta tcgacctgaa aaacaataaa gagaaactga gaaactctca
    14221 gtttgaatta tcataaagta caatctttta tttttcaagc gagaggttct tttagaaaga
    14281 ggaattcgca atgaaagtat ctcaaaaaat catattatct attttgttac tgcttacatt
    14341 agtagttggc tatttttcga ttgagtttat ccatggattt tcgtctgcaa aacagagttc
    14401 aactgtgaaa aaggtagagc cgaaaagtat ccctaccaca ctaaatgtgg ctttgattgg
    14461 ttcagatgct cgttcgaaag acgaaaacgg tcgctcggat tcacttctgg ttgcacaata
    14521 caaccagaaa acacaacaag caaaactgat ctctatcatg agagattcat atgtcgatat
    14581 tccaggttac ggaatgaata agataaatgc agcttactct tatgggggag ttgatttatt
    14641 aaatcaaaca ttaaaggaaa atttcaaatt tgaagctccc tattatgcaa gtatcacatt
    14701 tcaagatttt atcgattgcg ttaatgaact gtttcctggt ggagtcaaga tcgatgcaga
    14761 aaaatctcta gatttagatg gcgtatatat aaacaaagga gagcaagtga tggatggtaa
    14821 tactttactc cagtatgctc gatttcggga agacaaagaa ggagacttcg ggaggatcag
    14881 aaggcagcag caagtgatca aagctatctc tcagcagctg aaagacgtaa cttcaatatt
    14941 caagctaccc aaagcggttg ggaaattact tggtagtatc caaacgaacc ttccagaaag
    15001 tgtgcttctg gattgtggta tggatttcct caaagacata tgttagaata aacaggtata
    15061 aatagtgaaa taaaggaatg acaagcaaga gaaggaggaa aaaatgaaaa gaagtgacaa
    15121 gcacggcaaa aatcgaacag gcgcttatat tgccggcgca gtgatcttaa tagcagctgc
    15181 gggtggcggt tatttttact accagcacta tcaagaaacc caagcagtag aagctggaga
    15241 aaagacagtt gagcaatttg tccaagcttt gaacaaggga gattataaca aagctgcaga
    15301 aatgacatca aaaaaggcgg caaataaaag tgcattatct gaaaaagaga tcttagataa
    15361 ataccagaat atatacggtg ctgctgatgt caaaggactt cagatatcga atctaaaagt
    15421 agataaaaaa gacgattcta cttacagttt ttcatataaa gcaaagatga atacctcatt
    15481 aggtgaattg aaagatcttt cttataaagg aacattagac agaaatgatg gtcaaaccac
    15541 gatcaactgg caacccaact tggtttttcc agaaatggaa ggaaatgaca aagtaagcct
    15601 gaccacacaa gaagcagcaa gagggaatat tatagatcga aatggtgaac cattagcaac
    15661 aacaggcaaa ctaaaacaat taggagtcgt tccaagtaaa cttggagatg gaggcgaaaa
    15721 aacagccaat atcaaagcga ttgcttcctc cttcgactta acagaagatg ctatcaatca
    15781 ggcgatttca caaagctggg tacaacccga ttactttgtc ccattgaaaa tcattgatgg
    15841 agcaacgcca gaacttccag ctggagctac cattcaagaa gtagacggca gatattatcc
    15901 tttgggtgaa gcagctgctc agctgattgg ttacgtggga gatatcacag cagaagatat
    15961 tgataagaat ccagaattaa gcagtaatgg aaaaatcggt cgctcaggtt tggagatggc
    16021 ttttgataag gatcttcgtg ggacaacagg tgggaaatta agcattacag atgcagacgg
    16081 tgtagagaaa aaagttctga tcgaacatga agttcaaaat ggcaaagata tcaaattgac
    16141 aatcgatgcg aaggcacaaa aaacagcttt tgacagtcta ggaggaaaag ctggatcaac
    16201 tgttgcgaca acgccaaaaa ccggtgatct tcttgcgctt gctagctctc caagctatga
    16261 tccaaacaaa atgacaaacg ggatctcaca agaagattac aaagcttatg aagaaaatcc
    16321 tgaacatcca ttcatcagcc gatttgcgac aggttatgct cctggatcta cgtttaaaat
    16381 gattacagca gcaatcggtc tcgacaacgg cactatcgat ccaaatgaag tgttgacgat
    16441 caacgggctt aaatggcaaa aagacagttc ttggggatct tatcaagcaa cgcgtgtcag
    16501 tagtgatgta tcacaagtag acttaaaaac tgctttgatc tattccgata atatatatgc
    16561 ggcacaagaa acgttgaaaa tgggtgagaa aaaatttcgt acaggcttag ataaattcat
    16621 ttttggtgaa gaccttgatt tgccaattag tatgaatcca gcacaaattt ctaatgaaga
    16681 tagctttaac tcagatatct tgctagctga tactggatat ggacagggcg aacttctaat
    16741 tactcctatc cagcaagcag caatgtattc tgtttttacc aacaatggca cacttgtcta
    16801 tcctaaattg attgcagata aagagacaaa agataagaag aatgtcatca gcgaaacagc
    16861 agtacaaacg atcgtgccag atctgagaga agttgtgcaa gatgtaaatg gtacagcaca
    16921 ttctctttct gctttaggga ttccgttggc agcgaaaact ggtacagcgg aaatcaaaga
    16981 aaaacaggat gtaaaggggc aagagaacag tttcttgttt gctttcaatc ttgataacca
    17041 aggttatatg atggtcagca tgttggaaaa taaagaagat gatgattcag caaccaaacg
    17101 agcatcggaa ctattgcaat acctcaacca aaattatcaa taaaaaattg ctttttaagt
    17161 acagtaaaag gactgtggcg ttatcgtcac agtccttttt tcaaataaac ggttttcacg
    17221 atgtctcttt cacaacttct ttttgattta ctcacacttc ataaactttt tttgtaaaat
    17281 atttgagaac aagtggagaa ataagtgtac ttaataaaat cacgataact aaaggtgaat
    17341 agtactgtgg ttcgatcaat tgactttgct gtccaatttg taaaatgatc aaagccattt
    17401 ctccacgcga aatcatacca gcaccgacca tcaatgaact atgttgagaa aaaccggcaa
    17461 tctgagcacc aaaataacct ccaagcaatt tggtcaaaat cgctacaagc gtcaatatag
    17521 aaataaagag tagttgttca ctaaagtcag aaaaatcaac ttctaatcct acactgacaa
    17581 aaaatactgg tataaatact gcatatccta aagcctctac attgtggtat acttcttctt
    17641 taacctttgt ctgactgaca gctatcccag caaaaaaagc accaatgaca gagctaagcc
    17701 caacaagatc cgctacgtaa gacattccta aacaaataac tagcgatacg atgataatag
    17761 aagaatttgc gtacactttt tctgcaaatg aaagtaggaa aggcgcaatc catttgacta
    17821 ataagaaaat aaaaacaaaa taaattagct gttcaatcaa aacaagcggt aaagaagcat
    17881 tgctagaagc ttcccccgtt agaaaagata ggctgaaact tagaactaaa acgactaaaa
    17941 tgtcatctac cacagaagca cccaaaattg ttgatccttc ttttgtattc actacgttta
    18001 gttcttttaa tacttctact gaaatactga cagaagttgc cgcaagaata atcccaaaga
    18061 aaatcgcttc atttcccgaa acttggaatg ctttgcctgt caaccagcca aaaacaaccg
    18121 ggaagatgat cccaagcaaa gcaacgaaca taccaggtcg gaaatatttt ttcaaaagag
    18181 ataaatcact ttccagacca gctaaaaaca ttaataagat cacaccaatc tccgaaaagt
    18241 catggactag aatatcaggt ttgacccaat tcaatccagc ttggcctagc aaaacaccaa
    18301 caagtaattg tccaatcact gctgggatac ctaatctccg cgagatatgt gagccaatcg
    18361 ttgttgaaat caatatcagg cataaaatac caataaattc cattctcatc attcctttgt
    18421 ataaaatata gcccgaactt agcataacat acattattga aaaaatagga aaatatactc
    18481 acaaaaaaac caacaatcca aaagattttt ttggattgtt ggtttttata cggtctattt
    18541 taataactaa aaatgattaa cgtatagtca tttttcttat tctgatgaaa ttgataatct
    18601 cctggatgcc catgaaaatc aaaataaagc cgaatatttg gaatagaagc atcaaacttt
    18661 tgaatggatt gaacaataag acgaatccgc caatcaacaa cagaatacta ctataaacac
    18721 tccaactttt agcttttgta ttcatgctga agaacaactg gaacaagctg ttgatgataa
    18781 cagctaatcc aagaagaatc ggcaggatag aaacgattgc aggtgcaaaa gcatagacga
    18841 tcagagctaa gatcaggaaa aaaagaccac tgaacagacc gatactccaa gaacctgttt
    18901 tccttttaaa acggtaagcc tcaaaaagat tcaacaaccc aagcaaaaca agataggcgg
    18961 tgatcagata accgataaaa tgaaaaacag cagaagggtt tagcacaatc gcaatcccag
    19021 aaataatata aacagcagct cttaataagg cgtatttttg aatattttgt ttaatcgtct
    19081 caaacatata agaaaacctc ctttgactta ttttaacgtg aactagtcat tttaccaaat
    19141 taaatgttac aaaatgagcg aaggagaaga atatctctgg aaaaatcaac ttctgaggat
    19201 cttttccatt tttttgcctt ttgctagctc atcgatcatt ttatctaaat aacgaatttc
    19261 tttcatcaag ttatcctcga tgtcttctac tcgtacacca caaacaacac ctgtaatcaa
    19321 ggaacgtgaa ggattgggga caggggcttc cgcaaaaaat tgttcgaaag tcacttgttt
    19381 ttgtagttga tattctagtt ctgtttggga ataacccgtc agccaacaaa tgatttcatc
    19441 tacttcctct tttatacgat cttttctctc cgcttttgct acatacatag gataaacgct
    19501 tgcaaaactt gtcgtaaaaa tacgattttg cgccataatc agtcaccttt ctttttttca
    19561 aattatttac tacttatagt atataagaaa aagagaagaa aaagcgatta ttcaaaaaaa
    19621 caaaaaggat tgtgacaaaa ttcttgtcac catccttttt tccgctctct gactttttac
    19681 aatcttcata gctcaagtgc gcatcagata atcgaatgct cctaaagctg cagtagctcc
    19741 tgaacccatt gaaacaatga tttgtttgta tggactatct gtacaatcac cagctgcgaa
    19801 aacgccgggg atattcgttg cgccattttt ctcggtcact atttcatttc ttggattcat
    19861 ttcgatcgtt ccatctagcc aatccgtatt agggagtaag ccgactagaa tgaatactcc
    19921 tgaaacagct agtgtatgca tctccatcgt acggcgatcg atataggaga gagattctac
    19981 ctgattcgta ccgctgattt ctttcgtcgc tgcatgtttg ataacagtga cattcgcaag
    20041 agagactagt ttatcctgta agatttgatc tgcttttaat tctggtaaga attctagaac
    20101 atatacatgt tttgctaacc cagccaaatc gattgctgct tcaacaccag aattaccacc
    20161 accgataaca gcaatctctt ttcctttaaa taaagggccg tcacagtgtg ggcaataagc
    20221 aattccttta tttttaaact ctttttcgcc tggtacattg atagaacgcc agtgcgcacc
    20281 tgtcgcgatg atggcggttt ttgctttaag tgatataccg ttagctaagc caatattaat
    20341 aaaatgttgc tctttattta aggaaacggc ctgttgattc atcataatgt cgatagggta
    20401 actacgcaca tgactttcta cttgctgcat caattttgga ccttctgtgt aagaggtacc
    20461 aatcatattt tcaatcccta gtgtttcaac gacttgtccg ccgaaagaat cagcgacaag
    20521 tcctgtacgg attcctttac gtgctgcata gatagctgca ctactagctg ctggtccacc
    20581 gccaatcact aatacatcat aaggttcttt ttcaaacaac tcttcttcaa tcaaagggcc
    20641 agtgattttt tcaacaattt gttcgatcgt catgcgtccg ctactaaatt cttctccatt
    20701 taaaaacaca gttggcactg ccatgatttt tttatcatcg atttcttttt ggaacatccc
    20761 accttcaatc atcgtatgag taatatttgg atttaataca gctaatatat tcaatgcctg
    20821 tacaacgtcg gggcaattat gacaagtcaa gctaacataa ctgtcaaaat gcaatgtttg
    20881 tgttattttt ttgattttaa gactcaaaga ttcactgatt ttcggtgccc gtccactaat
    20941 ttgtaacata gccagtaaaa aagaggaaaa ttcatgaccg agggggatac cagcaaaaac
    21001 gattccactt tctttgccaa ctggattgat agaaaaacta ggtgtgcgca tcgaatctct
    21061 tttttcccaa gagattttag gagacatttc tgctatttcc ttcgtaaatt cagctagttt
    21121 ttgactattt tcactattat ccaaactaac agaaaaaaca ataggagatt caatcagtgc
    21181 taaatattgt tcgagatttt gtttagtttc tttgtctaac atatccacca cctaaatctt
    21241 tccaacaaga tcaaaacttg gtttcaatgt ttcgccactt tctttccatt ttgccggaca
    21301 aacttcacct gggtgggtac gtacatattg agcagcgcga atcttatcaa tcagtgcact
    21361 agcgtcacga ccgattccat ctgcattgat ttccatagct tgaacgattc cgtctggatc
    21421 aataataaat gttcctcgct gagctaatcc ttgctcttca tccaaaacat caaaaatacg
    21481 agaaatctga tgagacggat caccgatcat cacgtattcg attttaccga tagcatctga
    21541 tgtatcatgc catgctttgt gagtaaagtg ggtatcggta gagcaagaaa agacttcaac
    21601 acctaaagat ttcaatgtac tgtattgttc ttgtaagtct tctaattcag taggacaaac
    21661 aaaagtaaaa tcagctggat agaagcaaag aatactccag ttccccataa gatctttttc
    21721 actgacttct aaaaattctc cttgatgata agcatgtgca ttaaattctt cgattttttt
    21781 cccaatcaat gacatagtaa aacctccaat aataattatt tatttataat tattgtaatt
    21841 tagttggagt tgcaaagtca atggatattt tgttatttaa taaagattac tcaaaaacga
    21901 aggttaatta taaaaataga agaggtcgca agaaaatgct actgctggaa atactcggga
    21961 taacgttgag taacagtttc ctgttctttc agcatcatgt gtaagtgcaa tcccgtttga
    22021 aatcgagctt cttctggttt tttatcaact aaagccttga gtaaatcata atgttgttcc
    22081 atgatcgttt cccatggaag cccctgaatg gtcactcgta accagcggaa acgttctaaa
    22141 tgggtattat ggtcatctaa ccagttccaa acttcttttc ttcctgcaat ttcgaaacaa
    22201 gcttcatgaa ataaattatc tgcacggaaa aaagcgcgtt cgtcatttat tttgactgct
    22261 ttttcttgtt cttctaaaat cgtttctagc actattcgtg tttgtgtgtc caatttggaa
    22321 caacattcaa tcatgatttg ctcttccaac tgttctcgga caaagcgagc attttctgca
    22381 gaaacaagat taattttgga aacatacgta ccactttgag gtatcgtata cacgagttct
    22441 tgctgacgta gttgaatcaa tgcttcgcga atgggagtcc ggccgatagc aagatgttcc
    22501 tccagtgcct tttcagatat tttccttcca ggttctaagt cagcatagat aatttgttta
    22561 cgaatcgctt gataagcttg tgattgcaag ctgcttttca tctttattcc tccaaaatca
    22621 tttctatgaa cgtagtataa cagaagaggt ctgaaaaaca atctggtatg tcacattaac
    22681 tagtaaattt gtataaatag taaaattttg gttgacatag cgatttttat tgcataaaat
    22741 tcacttgaat acaaaaacta atatgttagt ttaggagggg aaaaatgatt gatttaaaca
    22801 actggaggaa ctttatccct gaattatctc aaaaagaagt agaaatacct cattttgacc
    22861 gtgcatcatt aagagaaaat ggacaaaaaa atcctgtctg gatccatttc ggtggcggaa
    22921 atctttatcg aggatttcat gcagaaatcg ctcaacgttt agcggatcta ggcgaattgt
    22981 ccactggtgt cattgtttgt gaaacattcg atgaagaaat aattgaaagg gcttaccaag
    23041 attttgatga tttgattctt gaagtggtca tgccaagcag tggagagttc gtcaaacgta
    23101 tattagctgc gacagctgac agtctgtatt gtcatccaga ttgttctgaa cactttgata
    23161 agatgattcg acactttgaa aatcctagtt tacaattagt tacttttgct attactgaaa
    23221 aaggttatcg attgaaagat acaaataata aatatatagg accgattgca gtcgatataa
    23281 aaaatggtcc aaagaagcca tcacatacaa tgagtattct tgctgtgcta ctttataaac
    23341 gttttttatc tggagcttgg ccgattgcac ttgtctctac agataatttt tcacaaaacg
    23401 gtagtcaatt acgtacttct ttgctaacga tcgcaaaaga atggcaaaag gaaggttatg
    23461 tacaaaatac atttatccaa tacttatcga atgaagaaat tgttgcgttt ccatggacga
    23521 tgattgatcg tatcaccccg aatccttcct tgtcagttag caaaatgttg gcaaaagagg
    23581 gcttttcaaa tatgtcgatt ttccaaaccg caaaaggaaa taatattgct ccatttgtca
    23641 atacagagga catccattat ttaatcatgg aagatcattt tcccaatgga cgtccgaaat
    23701 tcgaaaaagc tggggtattc ttgactgatc gtgcaacggt agataaagca gatacgatga
    23761 aggtgacgac ttgccttaat cctttgcata cgtctttagc tattatgggt tgtttactgg
    23821 ggtataggag catagctgag gagatgaaag atccagattt atcgctgcta gtttctgaaa
    23881 tcgggtacaa tgaaggcctc ccagttgtgg agtctccagg tatcattgat ccaaagatct
    23941 ttctggaaga agtaattact gtgcgctttt ctaatccaaa tattcctaat atgcctcagc
    24001 gaatcgccac ggatacctct caaaaggtgg cgattcgtta tggtgagacg attaaaaaat
    24061 atatggaaat agaagttaaa gacccgcaga cacttgtatt cataccattg actctcgctg
    24121 cttggcttcg ataccttatt ggcattgatg atcaaggaaa accattcatc ccgagtccag
    24181 acccactatt gacagacctt cagcataagc tttccgatat tcaactgggt acgcaagata
    24241 catcacatat tcatgagaaa atggctccta ttttgtcaaa taaagaaatt tttggtgtag
    24301 atctttacca agcagagctt ggcagaaaaa tagaagaaat gttcatcaag atggtagcag
    24361 gaccaaatgc cgtacgaaaa acattacagg attacttaca agaacatgga ggaaatttat
    24421 catggaaatg acttttagat ggtacggatc acaggaagaa aagattacct tagatgaaat
    24481 cagacagatt cctgcggtca aagggattgt aggcacattg atggatatac cagtaggaga
    24541 ggtatggcct agggagagga tcaaagaact aaaagaagaa attgagtctc gtgggttaac
    24601 actaaaagta attgaaagcg tcaatgtgca tgatgccatt aaaattggat taccttcccg
    24661 tgagcagtat atcgaaaact ataagcagac aatccgaaac ttggcagaat atggaataga
    24721 agtcatctgc tataatttta tgcctgtatt tgactgggtg aaatctgacc ttgactaccg
    24781 cttagaagac ggctcatcca cgttagcttt tatatctgat gatattccag atgatccaag
    24841 agaaatcgtc gcgcgcatcg aacattccag caatgaattt ggattgccgg gatgggaacc
    24901 tgagcgctta gctcctatca aatccttgct tgaagcctat gcatcagtag acgaagaaaa
    24961 acttcgagaa aattttgcct attttctcca atcaatcatc ccaacatgtg aagaggtagg
    25021 ggtcaaaatg gctgtacatc cagatgatcc gccatactcg atttttggat tagctcgaat
    25081 cgtaaagaat cgagaagatt tagattggtt gtgtcgcata gtagacagcc cttataacgg
    25141 aatcactctt tgtacaggga gtattgcaga agatccagaa aatgatgtat atgggatttt
    25201 agcagaattt tgccaaagag atcgaatccc atttgcacat gttcgaaata ttaaattttt
    25261 agctaatcga gatttctatg aggcaccaca taaatccgaa tacggttcat tagacatgta
    25321 ccgcatacta aaaacaatgt atgacaacgg atttaacgga tacatccgtc ctgatcatgg
    25381 aagaatgatc tggaatgaga caggaagacc aggatacggc ttatatgatc gagcattagg
    25441 tgctgtttat ctaaatggat tatgggaagc aatcgaaaaa ggaaacaaaa gtaaagataa
    25501 ttagtgaaaa aaactgtaat tttattatgt aaataagaag gatgttcaaa ttcttgatac
    25561 ataaatccgg aggttgttaa taagcattcg gatttatgta tcttatttgg tctaggaagt
    25621 aacttgattt tcttttttcg attgatccat gagtgcttct tcttttaatt caaagatata
    25681 ttctgagatc ctcataatca cttttttcca gtcttctggt gtgatgattc cttttaaaat
    25741 atagattttt ttgatatgag aaggcaattg tttcaaaggg cagtcgctga taacgatgtt
    25801 gcagttctcc ataaaatcat tttgtatttt gatcgattca tttgaaaaaa tttgttctat
    25861 cttaacgatc agatctttac tgatataaaa atcacgcgta tagttcacac caatcacaat
    25921 tcgcggctgt tttttgtagc gatcatacaa atgtactagg tcttcaacca tccattgcac
    25981 attttccttt tctaaaataa ctggtaacaa gtgttgattt tgttctctga aatccatata
    26041 aaattgttca atggcggttt ggacttcttt caatacgcta tgttcttgat aaagcatgaa
    26101 agtaggaagg ggcgtatttg gttgattgaa gttaagaaat ccattataaa gtttatttcg
    26161 tgcgaatgcc aatgagtaaa gaatcttttc tttttcaggt ataaacaaat caaatacagt
    26221 acaaagggac tccaccattt ctcttgaata aagaacatga ggaacgtttg ctttttttaa
    26281 aagtttgtat tgctccaagc ttgtttcttc cgattcgatt ttcgtgatag aaagtcgagc
    26341 taaaatattc actaagatgc gttgttcgcg acaaattggc agattaggtc gcaaaatatc
    26401 tacagagaga tcaataaaaa agtcaatata ttcatactct gtgaattctt tttgtacgtc
    26461 tttcacagaa gttgcaggaa aattctcttt acatattttt aatagaaaat ataatttatc
    26521 tatcataccg ctatttagtc ggtcaagtac ttctttttta aaaaaagtat tcatcataaa
    26581 agccactact tgcggatctt catcataaaa ttctgtagca gtacttgaaa agatgttcca
    26641 ataaatatct aatgctgcat attgaatttg tacctctttt ccagaaaaag actttctgcc
    26701 attattagaa aaggagatag acaaaccgta ctatgctaaa aatgcattaa tctttttcat
    26761 tttcgtatac aaataagagg cactatagtt tgtttgctga ctgaaactga taatcgaatt
    26821 tgtctgacag ctgaacaaag aagtcaagat ctgataactg actgattcgt tgcagtatct
    26881 tccaaataga cgataatagc agtaagccgg atcaattcct tcaaaattca actttaaaaa
    26941 aggtttttgc ttatcaatat gcagttctgt tagattgaga cgctcaaagt cttgttctaa
    27001 ttctttgatg tatagtaaaa aatttgattc agacatatgg aaatgatctg ctaaatattg
    27061 tgtagtaata aagaaacgat gttcagatag gaaaagaaaa atatctaatt ttgtattgct
    27121 ttttttcttc aagataaacg ttttaatcgc ttcttgagtt tgcatgaaag aggctccttt
    27181 ttacttaaat tgttaaaact tga
//
LOCUS       DACDUU010000037        26153 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.037, whole genome shotgun sequence.
ACCESSION   DACDUU010000037 DACDUU010000000
VERSION     DACDUU010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.037"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            133..3660
                     /locus_tag="IWL30_001560"
     CDS             133..3660
                     /locus_tag="IWL30_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP9706323.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="IWL30_001561"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="IWL30_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAP9706324.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="IWL30_001562"
     CDS             7448..7789
                     /locus_tag="IWL30_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9706325.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="IWL30_001563"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="IWL30_001563"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAP9706326.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIVRKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="IWL30_001564"
     CDS             9146..11566
                     /locus_tag="IWL30_001564"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAP9706327.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IWL30_001565"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IWL30_001565"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAP9706328.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="IWL30_001566"
     CDS             13000..13737
                     /locus_tag="IWL30_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9706329.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="IWL30_001567"
     CDS             13751..14566
                     /locus_tag="IWL30_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP9706330.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="IWL30_001568"
     CDS             14579..15226
                     /locus_tag="IWL30_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9706331.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="IWL30_001569"
     CDS             15242..15898
                     /locus_tag="IWL30_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9706332.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="IWL30_001570"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="IWL30_001570"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAP9706333.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="IWL30_001571"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="IWL30_001571"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAP9706334.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="IWL30_001572"
     CDS             18240..18758
                     /locus_tag="IWL30_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP9706335.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="IWL30_001573"
     CDS             18865..19365
                     /locus_tag="IWL30_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9706336.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="IWL30_001574"
     CDS             complement(19645..20565)
                     /locus_tag="IWL30_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAP9706337.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20730..21743
                     /locus_tag="IWL30_001575"
     CDS             20730..21743
                     /locus_tag="IWL30_001575"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAP9706338.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="IWL30_001576"
     CDS             complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="IWL30_001576"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAP9706339.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22828..23268
                     /gene="fabZ"
                     /locus_tag="IWL30_001577"
     CDS             22828..23268
                     /gene="fabZ"
                     /locus_tag="IWL30_001577"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP9706340.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23383..24144)
                     /locus_tag="IWL30_001578"
     CDS             complement(23383..24144)
                     /locus_tag="IWL30_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9706341.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24333..25427
                     /locus_tag="IWL30_001579"
     CDS             24333..25427
                     /locus_tag="IWL30_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP9706342.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            complement(25513..>26153)
                     /locus_tag="IWL30_001580"
     CDS             complement(25513..>26153)
                     /locus_tag="IWL30_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HAP9706343.1"
                     /translation="LYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPD
                     NYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWF
                     SRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNG
                     LPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
BASE COUNT         8643 a         4531 c         5508 g         7471 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg tgcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata
    20641 actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat
    20701 gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt
    20761 cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag
    20821 gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg
    20881 gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct
    20941 ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa
    21001 tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt
    21061 ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg
    21121 gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag
    21181 taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc
    21241 gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac
    21301 ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca
    21361 cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa
    21421 aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc
    21481 agttagctga agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc
    21541 tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg
    21601 ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa
    21661 caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag
    21721 gctggattga agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa
    21781 aaatcaggaa gagactttaa aaagtttttt cctgattttt atatgtttta ctttaaagaa
    21841 tgcaacaaaa gtttatgaaa gatggacacc tttgtcaacg tagataatat caccgatgat
    21901 tccgctggaa agtgggctga ttaagaaagc acaagcatta ccgacttctt cgatcgtcac
    21961 accttgctga tcaggcgtac gagattcaga taactggatc agtttttgat agtctttaac
    22021 acctgtgaca gctaatgttt tgattgcccc agctgagatt ccattgaccc gaattccttt
    22081 aggcgagaac tcagctgcca aataacggat agcagcttca agagaagctt tcgcaatacc
    22141 catcatgtta taattcggga ttgcgcgttc tgaacctaga taagtcatgc taacgatccc
    22201 gctgtttggt gcaagaaacg gttgtgcgta ttttgataca gcaattaaag agtagctgct
    22261 gatatcttga gctaattgat agccgtctct ggaaatatca gacacatttc cggacaattc
    22321 ttctttgttg gcgtaagcaa ctgcatggac aaggccgtgg atctccccag catattcacc
    22381 gatggcattc attgcttttt cgatgctttc gtcgctagaa acatcacatt ctacagtgaa
    22441 tgcttcttcg ccgaccaatt tgactaatga ttttttcatt cgttcatttt ggtaagtata
    22501 gataatttct gccccttgtt tcaacattgc ttcagcacaa ccccaagcaa tgcttttttt
    22561 gttagcgacg cccataatga cgattttttt tccttctaga aatgacataa tgactctcct
    22621 ttgtttctga taaaaatttg atagtcgtaa aaaaatagag aataatagct aaaataactt
    22681 cttgatttct taattattag tcaaaatgtt ttgattgtca aactattttt tcttaaatta
    22741 aaaaaagatt gctaaatatt taggaatatg atactttgat agtcaaagta atgaaaatgg
    22801 caattgcctg aaagaaggag tttaattatg tcttacgtat tatctgctac tgaagtaatg
    22861 gaattgattc ctaatcgtta tcctatttgt tatattgatt atgtagatac tttagaacca
    22921 ggaaaaaaga tcgttgcgac aaaaaatgta acaatcaatg aagatttttt ccaaggtcac
    22981 tttcctgaca atccagtgat gccgggtgcg ttgattctag aaacactggc acaagcaggt
    23041 tcgatactta tcctaaaatc agaagagttt ctaggaaaaa gagcttatat cggtgggatc
    23101 aataaggcca aattccgtca aaaagttgtt ccaggagatg tgctgaaatg tcattttgaa
    23161 atcaccaaaa tgaagggatc agtcggtaca gcgttatctg cagcatatgt agatgataaa
    23221 aaagtctgtg agtgcgagtt cacgttcatc gtcaaccaaa atgcttgact ataaaacctg
    23281 atatagcaaa acaaaaggaa gcttacttgc tttttatgta aaaagaagac gcctgaatcc
    23341 tagcatacgt tgtcatctat gcaggattca gacgttattt ttttattttt gttttgcttc
    23401 ttgcagtata ttgattttat cgatttttac ttcttctttt ggtttgtctg aactatctgt
    23461 ttcgatattt gcaatagcat ccacgacatc cattccttca atcacttggc cgaagacagt
    23521 ataatggcca tctagtgaag gataaccgcc gtttttataa gcgtcgatga ttgcttgagg
    23581 atagtcgtct tttaaaagac catcatgcat atcatctgta ttttgaacga taaagaactg
    23641 gctgccgtta ctgttaggat cttgtgagcg agccatagat aacgcacctc ttatattata
    23701 aaggtggttg ttgatctctg tttcaaatcc ttctccccaa atgctttccc ctccagtgcc
    23761 atcgccttta ggatcgccac cttggatcat gaaatctttg atgacgcgat ggaaagtcag
    23821 tccgttgtag taaccgtctt ttgcatgtgt cataaagttt tcaaccgctt ttggtgcgtg
    23881 ttcgggaaat aatttgattt tgattgtgcc tttattcgtc accatttcga ccagatcttc
    23941 attttcggcg acatctttcg tcaattgtgg taattccaat gcgttcaaat cgacgctact
    24001 ggcagtcgtg ctttccgcag tagatgtact ttttgaactt tctgtagacg aactagaaga
    24061 agtcgtgttg tttgcagaac aaccagcagc aaacagcaat gctgctgcta aactaatact
    24121 taaaataact ttatttttct tcataactgt tcctttccaa tttactattt tgttatcttt
    24181 aatgataaca aaatagaggg aaaataccta cttaaattat tggaatttat catttatttt
    24241 aaaagaaaaa gaaaaggtgt agaccattta ctttgtgttt gctataatgt aagtggatac
    24301 aaaaacgaaa agatgcagga ggaaatggta atatgggaaa attaggaatc tcgatttatc
    24361 cagaacggtc aacatttgaa aaggacaaag cgtacttgga tttagctcat aagtatgggt
    24421 ataaacgggt ttttaccagt ttgctgcaga tcaatgatga taaagaaaaa gttctttcag
    24481 aatttaaaga agtagttgat tacgcgaata gcctaggcat ggaagtaatg gttgatatca
    24541 atccagcgtt atttgaacag ttggagattt cttacgatga cttatctttt ttccataaga
    24601 tgggtgccta cggggtccgc ttagatatcg gattcacagg agcagaagaa gcaaaaatga
    24661 cgcgcaatcc ttttggtatc aaaatcgaga tcaatatgag ttcgggcacg aattacgttg
    24721 acaacatcat gagttattcg ccaaacacgg ataatttatt aggttctcat aatttttatc
    24781 ctcatcgata ctctggttta ggatacgaac actttgtttt ttgttcagaa aaattcagaa
    24841 aatataattt gaatacgatg gcattcgtca attcgcaatc tgctgagttt ggcccttggc
    24901 ctacgcaaga tggcttgtgc acgctggaag accaccgaga tcttgaaatt gcgacacaag
    24961 tgaagcattt aatccttaca ggattgattg atgatatttc tattggaaat gcgtacgcaa
    25021 gtgaagaaga attgaaagaa atggcagaag catttaacgc ggattatcca acattgaaag
    25081 tagatacgga agaaggtatt acagaaaacg aacgaatctg cttgtttgat aaccttcata
    25141 gttatcgagg ggatcgctca gaatatatcc ttcgttcaac gatgactcgt gtatactaca
    25201 aagataaaga tttcccgcct cataatacgc gagatatgca tcatggggat gtactgattg
    25261 acaatgaagg gtacggtcaa tacaaaggag aaacacaaat cgccttgaag gatatgaaaa
    25321 acgacggacg tgtcaatgtt gtaggaagaa tctccgatga cgaattattc ctccttgact
    25381 ttttgaaacc atggtcaagc tttaaattaa ttgaaaataa aaaatagata attagttatt
    25441 gcagaaataa tccgctagaa atagccagtt atttctgctt ttttattttg tttttttata
    25501 ggttatgatt tgtcaaatta agctagaata aaatgcttct tgtacatagc tcaaaaatat
    25561 ttcgattggt gttctgtaat tcaatgattt tcttggaata tgattacgtt gattgctgac
    25621 actggaaata aatgtctgat tcacttttct aaaatccatt gatttcggca gtccattacg
    25681 acgcagaatc ccgttagaat tctcgtttaa tgggcgttga gaaggtgttc cagggtccgc
    25741 aaaatatata tcaatatcat gttggttact aatggctttc cagttagaaa attcttttcc
    25801 acagtcaaac gtaatagatt taaagaagtt tttagggaag cgagaaaacc attgattaag
    25861 ggcagtttca atatctaatg ccttacggcc gttgggttta atcgtgataa tgactttaga
    25921 taatctttca actaaagtaa tgacggcact tttatgatga atgccaacga tcgtatcacc
    25981 ttcaaggtga ccaaactcag aattgaaatc aggataatta tcaggtcgat catggattga
    26041 gcgctgatac tgttgttttc cccgtttttc ctgatggcca ttgggttttc ttttaccttt
    26101 catcggtagt gtgtcaatat caaatattcc tttagaaaat aaacgataaa gtg
//
LOCUS       DACDUU010000038        24664 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.038, whole genome shotgun sequence.
ACCESSION   DACDUU010000038 DACDUU010000000
VERSION     DACDUU010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.038"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(366..1484)
                     /locus_tag="IWL30_001581"
     CDS             complement(366..1484)
                     /locus_tag="IWL30_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706344.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1955..3262
                     /locus_tag="IWL30_001582"
     CDS             1955..3262
                     /locus_tag="IWL30_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP9706345.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3381..4580
                     /locus_tag="IWL30_001583"
     CDS             3381..4580
                     /locus_tag="IWL30_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP9706346.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4607..5875)
                     /locus_tag="IWL30_001584"
     CDS             complement(4607..5875)
                     /locus_tag="IWL30_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAP9706347.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(6038..6684)
                     /locus_tag="IWL30_001585"
                     /pseudo
     CDS             complement(6038..6684)
                     /locus_tag="IWL30_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7092..7595)
                     /locus_tag="IWL30_001586"
     CDS             complement(7092..7595)
                     /locus_tag="IWL30_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9706348.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7718..8482)
                     /locus_tag="IWL30_001587"
     CDS             complement(7718..8482)
                     /locus_tag="IWL30_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAP9706349.1"
                     /translation="MKEIQSVKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSSTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8589..8864
                     /locus_tag="IWL30_001588"
     CDS             8589..8864
                     /locus_tag="IWL30_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAP9706350.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9580..10530
                     /gene="yidC"
                     /locus_tag="IWL30_001589"
     CDS             9580..10530
                     /gene="yidC"
                     /locus_tag="IWL30_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP9706351.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGGAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQATKQIKQPKNSTNNKGRNAGRQQKRK"
     gene            10652..11861
                     /locus_tag="IWL30_001590"
                     /pseudo
     CDS             10652..11861
                     /locus_tag="IWL30_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017466553.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            complement(12171..12392)
                     /locus_tag="IWL30_001591"
     CDS             complement(12171..12392)
                     /locus_tag="IWL30_001591"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9706352.1"
                     /translation="MFKNYTINQKILPLDMEQYISETDVAFAVNALVEEKIKVDLYVS
                     SKFTNVKVVGADLFVVNVRKQKVSETDKF"
     gene            complement(12553..13074)
                     /gene="tnpA"
                     /locus_tag="IWL30_001592"
                     /pseudo
     CDS             complement(12553..13074)
                     /gene="tnpA"
                     /locus_tag="IWL30_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631987.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(13165..13531)
                     /locus_tag="IWL30_001593"
                     /pseudo
     CDS             complement(13165..13531)
                     /locus_tag="IWL30_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875519.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
     gene            complement(13545..13830)
                     /locus_tag="IWL30_001594"
                     /pseudo
     CDS             complement(13545..13830)
                     /locus_tag="IWL30_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
     gene            complement(13836..14030)
                     /locus_tag="IWL30_001595"
     CDS             complement(13836..14030)
                     /locus_tag="IWL30_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706353.1"
                     /translation="MAETRPKGYPKLKEYTPSRFMLSECHYDKARADRAVNFIGQLRH
                     TKGKWAGNRFWLLPWQEQII"
     gene            complement(14030..14506)
                     /locus_tag="IWL30_001596"
                     /pseudo
     CDS             complement(14030..14506)
                     /locus_tag="IWL30_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004070864.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
     gene            complement(14974..15327)
                     /locus_tag="IWL30_001597"
     CDS             complement(14974..15327)
                     /locus_tag="IWL30_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706354.1"
                     /translation="MAMSEKAKKYLKEIKGAKTIPELKDVEIAIKRDGILAWAEFTKL
                     NEAVEEKKVALRKKKQETSLQEILFWAYKKESDKLLKMMDEGADKDAIQMQVQRYDSI
                     GQIIAEADLEDEYEV"
     gene            complement(15455..15994)
                     /locus_tag="IWL30_001598"
     CDS             complement(15455..15994)
                     /locus_tag="IWL30_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706355.1"
                     /translation="MQLLLYRIGDSTKIRFLINVRFRVIYSTVLPKGIPQRQKWMIHM
                     EEIMQMILGNAKFQLGQVVSTPAVLETFNPLQTANGIPEALSNLWFRQEISIAEIEDA
                     VLDAKNGKELANSLNRIKLFRKFTLDRELDGMVRLKGTDVWKNISYLKVYTATPPKKP
                     TPPRFQSNGWSLFFSHKSA"
     gene            16024..16215
                     /locus_tag="IWL30_001599"
     CDS             16024..16215
                     /locus_tag="IWL30_001599"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706356.1"
                     /translation="MIIRDDHIYTCDSCHYSFPADEQPERCPDCEKTATRLDTEIETE
                     DYYRVRAEIKAEIKALNAG"
     gene            complement(16257..17123)
                     /locus_tag="IWL30_001600"
     CDS             complement(16257..17123)
                     /locus_tag="IWL30_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9706357.1"
                     /translation="MEELFSNALKSVKSNRVLYTASFFARSSLLYLQEIGQLTALKAH
                     TSRRTGLSSYLCFLVEVGSGTLEYKGQKYTLQVGDMVFIDCLKPYVHESSEDDLWTLR
                     WCHFNGIGMTEVYKKYLERGGQPVFKTSKTDKYADLLRSLHMVAEGQEYVRDMKINVL
                     LSELLMHLMEDSWNPDKVPNFSTKKMELQNIKQYLDDHYTEKITLDQLSAEFFINKQY
                     LAKIFRETYGTTVVGYLNQKRITQAKHLLRFTNLSMEEIATKIGITDANYFSRTFFKV
                     EGVRPSEFRKMW"
     gene            complement(17147..18799)
                     /locus_tag="IWL30_001601"
     CDS             complement(17147..18799)
                     /locus_tag="IWL30_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP9706358.1"
                     /translation="MNYKVEYERWLNHANADIIEELRSYSEKDIEDSFYKNLSFGTGG
                     LRGTIGAGTNRMNVHTVGKASQGLADYLNKNYEAASVVVGYDSRIKSDVFAKTAADIF
                     AANGIQVHLWPELLPVPTVSFATRYLKADAGVMITASHNPSKYNGYKVYGADGCQITT
                     EAATAILAEIEKLDIFADVKMDGDASKIASIQSEVLTAFIEEVKGQSVLFGEEVDKNV
                     AIVYSPLNGTGLVPVTRTLAEMGYSNVTVVEEQRLPDGNFPTCPYPNPEIKEAMSLGM
                     EYAKKYNADLLLATDPDCDRVGIAVKSAHGDYVLLSGNETGLLLLDYICSQRIKHGKM
                     PADPVMVKTIVTMDLGERIASNYGVRTIDVLTGFKFIGEQIGMLEKAGKVDSYIFGFE
                     ESYGYLSGSYVRDKDGVNAAYLICEMFSYYKTQGISLLDKLNEIYTKYGYCLNTLHSF
                     EFDGAAGFTKMQEIMVEFHKGLDIIGGKKVIETLDYSKGLNGLPKSDVLKFMLEDNCS
                     VVVRPSGTEPKLKTYISVSAENKEAAEAMEAIMAAELAEMFE"
     gene            complement(18804..19706)
                     /locus_tag="IWL30_001602"
     CDS             complement(18804..19706)
                     /locus_tag="IWL30_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HAP9706359.1"
                     /translation="MMNLQKELRCLLLRKLGMLYAPGWTELCCVWSEYQGMTLTEVLQ
                     EHPEFLGTHPQTKGELPILIKFIDAKKDLSVQVHPTDEYAIEKENGQLGKTEMWYVLD
                     ASLIYGLQKITEEAVIREAIAQGTVERYLQKVPVKKNDVFFIEAGTIHAIGAGALIAE
                     IQENSNLTYRLYDYDRVDKNGKKRELHVDKALEVANLKGLSEPKQPLRVLKYRAGVAA
                     ELLCRCKHFEVSRMIVNTERRQTVHYRADSISFRVLLCVNGCGTVRFDNESLDIYKGD
                     CLFVPADSTEISIHGSIQFLDVRG"
     gene            complement(19790..21103)
                     /locus_tag="IWL30_001603"
     CDS             complement(19790..21103)
                     /locus_tag="IWL30_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP9706360.1"
                     /translation="MNIVLLSGGSGKRLWPLSNDIRSKQFIKIFKTEDGYESMVQRVY
                     RQIKKVDNDATVTIATSKTQVSAIHNQLGEDVGVSVEPCRRDTFPAIALATAYLVDVM
                     GIDPEESVVVCPVDPYVEDDYFEALKSLSDQADKGEANLVLMGIEPTYPSEKYGYIIP
                     ESAEQTAFVNTFKEKPSEAVAKEYIAQGALWNGGVFVYKLKYVMERAHQLIDFVDYQD
                     LFNKYDTLTKISFDYAVVEHEDKIQVQRFAGMWKDLGTWNTLTEGMEESIIGKGVMNE
                     NCDGVHILNELDVPVLAMGLHDVVICASREGILVSDKEQSSYIKPFVDKFEQQIMFAE
                     KSWGSYKVMDVEAESMTIKVTLNASHSMNYHSHKNRDEVWVVLAGTGKTIVDGYEQVV
                     KAGDVITMSAGCRHTIMADTELKLIEVQLGKDINVKDKQKFELEY"
     gene            complement(21171..22115)
                     /locus_tag="IWL30_001604"
     CDS             complement(21171..22115)
                     /locus_tag="IWL30_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HAP9706361.1"
                     /translation="MMEKDAKIYVAGHRGMAGSAIVRELNRQDYNNIITRTHKELDLC
                     RQDAVEAFFAQEKPDYVFLAAAKVGGIIANQNALADFMYENMILEMNVINSAWENGCK
                     KLQFLGSSCIYPRMAPQPMPESCLLTSELEKTNEAYALAKISGLKYCEFLNKQYGTDY
                     ISVMPTNLYGPNDNYHPTHSHVLPALIRRFHEAKEAGLPTVTCWGDGSPLREFLYVDD
                     LANLCVFLMNNYSGDETVNAGTGKELSIKELTEMVAKVIGYEGEILWDTSKPNGTPRK
                     LLDVSKATKLGWTYKTELEDGIRLSYEDFLNNPMRAER"
     gene            complement(22152..23165)
                     /locus_tag="IWL30_001605"
     CDS             complement(22152..23165)
                     /locus_tag="IWL30_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706362.1"
                     /translation="MATLCRCEAKITLVCGANHLDFAKRNLEQELPKELIDRVRYRPI
                     NTDIGLINREGTLLVDTGKLTEICEYFLAKVPSLAKTEAEWLKREAVSAVLCDMPIWA
                     MDACDMAGVPFLYVGNFTWTELYREFLPEHIWKEYAKRYGKIQHTMLYALNNEEMLEF
                     LQNSEKTPTSVVARPIHNDEVQRIKSQHEKPPVFVALGMSARFKQPIDVSHAPYDFIT
                     TLGVPLVGENVTVISNSTPNTQDYIAAADYVITKAGWGTVAECILAQRPMALFARDTV
                     LEDRTTICKLQKLGLAISVTPENLHRVERVIEDLKQLDTSNFDTFYDAASEIADKLIS
                     LMQ"
     gene            complement(23245..24291)
                     /gene="gmd"
                     /locus_tag="IWL30_001606"
     CDS             complement(23245..24291)
                     /gene="gmd"
                     /locus_tag="IWL30_001606"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HAP9706363.1"
                     /translation="MKKALITGITGQDGSYLADFLLEKGYEVHGITRRASISNTARID
                     HLMGKITLHDGDLSDSSSLIRIISIVQPDEIYNLAAQSHVQVSFDVPEYSGDVDALGV
                     MRILEACRILGLTKKTKIYQASTSELYGKVEEVPQRETTPFHPYSPYAVAKQYGFWIT
                     KEYREAYGMFAVNGILFNHESERRGENFVTRKITLAAGRIAEGLQDHLELGNMDSLRD
                     WGYAKDYVECMWMILQHETPEDFVIATGEQHTVRDFTEKAFAANGMTIRWEGTGIDEK
                     GYDVETGKMLVCVNPQWFRPTDVDNLWGDPTKAKTVLGWNPQKTSYEELVNIMAKHDR
                     ELAKREAAMKNAVK"
BASE COUNT         7005 a         5590 c         4704 g         7365 t
ORIGIN      
        1 tttaaacggt gttccagaag tagcttcttc ggaaataagc cgaaatttca caaaaatttg
       61 agaaataatt tttgtcaatc tcttcttatt tcttgaagct gaccacttct gtcacaacct
      121 cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccacca aacaaaaaat
      181 atgaagagct atttttgctt gctgttctta ttacttgatg cagaacagct ttttcacaac
      241 ctctcataca cggtgtttaa tcagctgacc ttcggtatta gctcaaaaaa cggaaaaata
      301 tgtgaaacta tttttccgtt tgtttctcta atactcaggt caaaacactg atttcacaac
      361 ctcttttaat aacgaagaat cgctgtcaga ctcttttcat ctggcgcatc tttttgatcc
      421 aaaataaaca ctttcccgcc attaatgatc acatcatctg ctaatgcatt caacacttga
      481 cgccgatcat attctgtttc aggatcttca ccgaaaccat cagctaagtt tgacgtagcg
      541 ataaataaat gtgacacttt tccttctttc gcagctggaa cgatatcaac taactgatcg
      601 atgaatttct tatctaagag tttattgtat tcttttgatt cttgagctgc gagttcactt
      661 gacagcttct tcgccccttc ttcgatatct ctaaaggata gctgcgcagg agaagcagaa
      721 atcgcaacgt ctgcacaata atagggattt ttagcaattt ttttgaacat cgtctgattt
      781 tctggtaatg caaataaata aatcggccat tgttcaggat tttcgaaaga atccttcaag
      841 tacgtatcaa cagcttggta atagttgacc caatcgatct ctacttcttc atcttttgga
      901 tttacgccat ggaaagttac tccatctcgg gctccgcctc ctccattaga agagaagttc
      961 aaaccgccac ctgtcagttc agatcccaat gcagtaacga catcaaccgg tgccccttct
     1021 ggtagagata cttcttttac ttgtttgttg ctgacctcat acaatttcat ggagtcgcgg
     1081 ttcaatccca ataaataata agaatagttg aactgcccat ttttgataat agccagtaaa
     1141 taaggaagat cacttacata gtattgatcg tctaccggga tactcaaacg atggatatag
     1201 gtctggtcag cagttataat gaccgctaca ctcgtagttg cactacgcca aaacgaagga
     1261 tctcctaaca aattttcaat tttttcttgg aacggggacc agtcatgttc ggaatatttt
     1321 ttctcaaaac gtttctttgc ttctttcgca aaatttttga atttgatctg atctttttcc
     1381 acatcttgat gggcgacatg cgtattcagc atgatcgtga cgaatggtcc tttagcgcct
     1441 tctgaagtca attgtgtcaa taattcttta tccttttttg ccataaacgt acccctccta
     1501 aaaaaatcat tcctttgttt ctatctaaag tctaccacaa cactattttt ccatacaata
     1561 aaacgtatct aaaaaaataa aacggtttct acggttttaa atttagctat ttttttaagt
     1621 tatcttcata atttcctcaa ttatttagcc ccagtcttca actattcttc atgaaatgct
     1681 gataaactaa gaaaaagaat aaaagaggtg gatcttatgc atttgattat gattttcagt
     1741 ttattaagta ttatcagttt atttgctttc agcgcttccc gcttattaga tgcggaaaca
     1801 cagcctattc ctgtaaaagt acgtaaaaac gaccgctaaa cttccataac gcgcgttctt
     1861 atcctaaagt tttttcagaa acattgaaac tctctcccct ctctgataaa attttaattt
     1921 gtagcgaaac aaattaatta caaggagatc ggatatgtcc ataaaaaaaa cacgtctagt
     1981 cctattattg gctagtctca ttctttgcct tgcttctttt ctaccagctg ttgctgtgca
     2041 agctgaagat acgttcaaag taaatgcaaa agcagctttt gctgtagatg cgcaaagcgg
     2101 taagattctt tatgatcaag atggagaaaa aaccatgggg atcgcttcaa tcacaaaaat
     2161 catcggtctt tacatcgtcc ttgatcaagt aaaagaagga aaactgtcat ggggtgataa
     2221 agtttcgatt tcagattatg cagaaaattt gagtatcacc ccagatttat cgaatgttcc
     2281 tttgcataaa gaaaacacct acactgtcaa agaattattc gactctgcaa tcatccagtc
     2341 agccaatgct tcaatggtgg ccttggcaga aaaaatctct ggcagcgaag ctaaattcac
     2401 tgaacgaatg aaagaacaat tgaaagattg gggaatcaaa gatgcaacaa tcgttaatgc
     2461 atcaggattg aacaactctt atcttggcga aaaccgacca gaaggcactg gagaaaacga
     2521 cgaaaatcaa atgtctgctc aagatgtggc catcgttgcc cgccatttga tcttggattt
     2581 cccagaaata ctggatgtat caagtactac gacccaaatg tttggcgaaa atacgcaatc
     2641 accagtggaa atggtcaact ggaactggat gctcccagga ttcgttaatt acaaggaagg
     2701 cgtggacgga ttaaaaactg gtacgactga gcttgcaggt gcttgtttcg ttggtacgat
     2761 cactaaaaac gatcaacgga tcatcactgt cgtattgaat gcggacggcc atgcggaaaa
     2821 cccgagtgcc cgcttcaatg aaacagaccg tttgatggac tactgctatg acaattggtc
     2881 agaaaaagag ctcggaaaag ctaacgcaag tattcctagt ctaaaaacca ttgatgtaaa
     2941 agacggaaaa gaatcaacag ttcctgttgt gcttaaaagc cctgtaaaag tatgggtacg
     3001 aaacgatatg gatactggca aattaacgat cacgccaaca cttgacaaga agcaagtcac
     3061 tgacaatgag atccaagcac ctatggataa aggtacaaaa atcggtaccg ctacgatcac
     3121 cttggcagac gataaattag gctatttaga agacaacaat gcaccatcaa cagagatcat
     3181 tacggataaa acagtggaaa aagcgaatat attcgtgatt ggctggcgac gcgtcgcaga
     3241 atttttcggc aacttgtttt aattgaacaa taagaaaaac atctttccat cggaaagatg
     3301 tttttttgct ttttctagtt taaataacag cttttttcat ctattttcgt tatactgtat
     3361 aagaaaaagg agttgaattt atgaatctaa ccagagatct gatcttttta gttctctatc
     3421 ttattggggt attctctatc gtttattggc gaaaacagaa gaaggatcct tccttgttct
     3481 tttacggcag tacgtggaca tttacggtaa tcttaactta ttttgtcgtg ttggaaattt
     3541 tttattcgac cttttatttt ttgattccat tgttgttctt tagtatcttt gctttttctt
     3601 atttcacaga aaaacgaagg ctactgaatg gtcttttatt caatgtttta ctcatcagtt
     3661 tcggcatcta cttgtttgtc ttgctgtatg aaacgcaaaa tatttttctt ggcggactga
     3721 tcgccttgat cacgattcca ttgctacttg tccttttatt tggtatttat ggattgattg
     3781 tttttctttt ttggaacggg gtcacagttt tacgtagaga atcgcattca ttagctaatc
     3841 ttttaacgtt gatactagcc atttttctta cactgtttct tgtttttgac tttttcttgt
     3901 taaaatattt gccgcaatgg ataaatgctt tgtttttctg cgttcccctg atcttgatct
     3961 atctatttat cgtattctac aactttttga ctgtctcatt cttgtatcag ttcaaccggc
     4021 ctcgatacaa tcaagacttc atcgttgtgt taggcgctgg attgatcaat ggagaaacag
     4081 tttctcctct tctagccaag cgaatcaata aggcaattgc tttttatcgg gctcaaagcc
     4141 gtgctacatt aaatccgccc atcttattga tgtctggcgg gcaaggagca gacgaaaaag
     4201 taccagaggc cattgcgatg aaacaatatg ccatggaaca aggaatccct gaaagagaca
     4261 ttcttgtcga aaccaattcg acaaccactt tggaaaacat gctctattca aaggaaatca
     4321 tggatcagca aatgaaaggg ccataccgcg cgatctttag ttccaacaat taccacattt
     4381 ttagagccgg tctttatgcg cggcaagcca aattgaaagc aaatggtatt ggtgcaaaaa
     4441 cagctttcta ctacttgcct aatgcgtttt taagagaata cctcgccatc ttgatgatga
     4501 acaaaaaaag acatatgatc atttgcggat tgcttttcgc aggctccgca ttcttatcgc
     4561 tgattacttt acttttctaa aaaaacaagg tgtgacaaaa catatgtcac accttgtttt
     4621 tttctttcat tctcttaact attggcaaaa agagtatccc ttttttgacc gctacttgct
     4681 gagcagaata aataccgctg ttgcctgaac acataaagct gatcaagcag atcataaaga
     4741 agtatactgc cgcttgtgaa ccgaacaatt cgatccccat cacaaagcag gcaatcggtg
     4801 tatttgtcgc accagaaaat actccgataa agcccaatcc tgcaagaaaa ggaacagaaa
     4861 catggagcaa aggcgctagt gctgcaccaa gcgttgcacc aatttcaaac aatggtgtca
     4921 cttctccccc ttgatatccc gcgcctaaag acaaaactgt gaagaataac ttcccaataa
     4981 aatcatagac atgtgcctgt ccattaaatg attcttgaag taacggaaga ctcaatccga
     5041 gataacgttg tgtttgcaaa atcaatgctg ctgccacaac tacagctcca cctatcgtat
     5101 ttcgacaaac aggatgagga atccattgtg catatcgcgc ttttaaaaag acgatcgaac
     5161 ggctgaatac ccaaccaatc aaaccaaaag caatcccagc aacaaaaact ttgaagaaca
     5221 gcaacatact ccattcagga atcacaccca tttggtaatg tagatgagaa acaccatagc
     5281 tttctgtcat gaaatttgca aacagtgcag caaagaagct agggaagatc gcttctgttc
     5341 ggactttccc gattgccaac acttctaatc caaataatgt tccagctaaa ggtgtgccaa
     5401 atacagaact aaaacccgca ctgattccac tgataatcaa gatttcccgt tctgatttat
     5461 ccaatcggaa caaacgtgcc gtattatctg ctaacgctcc ccccatttgg actgctgttc
     5521 cttcacggcc aacagatccc ccaaataaat gggtagttat ggttccaaac agtgtaagtg
     5581 gaataagccg caacgggatt ttttcttctc caccatttcc ttgatcgatc actaagttgt
     5641 ttccacgact agaaagtccg ccgtgatacg catagagata agcaaaaagc gcaccactaa
     5701 ctggtagtag acaaaggagc caaggatgtg atagtcggat atctgtcaca actgtcaggc
     5761 tatttaagaa aaaagcagac aagctcccca tgaatatacc gagaatcgct gtgatcacca
     5821 gccacttcag catatacact aaaatgcgta ctggactcgg taattgtttt tccataaaaa
     5881 attcctcctc aataaaaaaa cgcctactaa agaaaggggc tgtccccaga ctttagtagg
     5941 cgtcattagc tatctttcgt tagcatttaa aggcgaacac catcacctat tcacttccat
     6001 taaaagatag catatttttc tgtaaaaaac aataaaatta tcgttctttc tttacacgtt
     6061 cagcaaattc atagaaagga gccaatttcg ttaactgttg attacattgg tcactatcat
     6121 gacaaatata attccccttt ttcgtatacg tgccatcaga acctgctttc gttgtagaaa
     6181 gaaaaagcga aacattcgat gttttctgac agattgcaca gattccctta tttatcgttg
     6241 gtgataaagt accgctaatc ccttttaatt tttcatcttc gtaatagacc atgaattttt
     6301 tctgcgtacc tggatcattc cagccgatgt acgtatgttc ttgcaagtcg atttcattcc
     6361 aagctggttg tttgagtttt ttcacttttc ggaataattt ctcgatttgt ggtttgctag
     6421 gctgcataaa cggctgaacg atttgcttca gctcctccaa tgctctttct gcttgcgctt
     6481 ttgtcagttt tacgtctaaa actgtcgtta aaaatgtatc gattctggca gttcgtgatc
     6541 taatatgtct cgaatctttt cagctgctag tgcctgaact gtttgtaccg tttttggatc
     6601 attgacagat gagtacgcat ttaataactg ttctacttct cttattataa aaaaatattg
     6661 gtatggtttt attgtttgtt ccatgatttt aaagtccttt cttctaacca aagtgcatac
     6721 tttgtagaaa aaagtgatct tctgcaaagt atgtgttacc tgaagatcct ggactctttt
     6781 aaataagata cgttttgcat cttatcatca ccccctttaa tgcttattat tctatgtaca
     6841 tgattcattc gataaataaa agtaaaccgt atcttgaaca gatagtcaac tgcaaacaga
     6901 aaaaaggact gtgacaaaag tcgtgtcaaa gtcccttgtt tcgaatgaat ggtgttcaaa
     6961 agcttgcatc tgcggtaata agccgaaact tcacaaaaat ttgagaagta attttcgtaa
     7021 attccttctt atttcttgaa gctgaccact tctgtcacaa cctcaatatg gtctattatt
     7081 ggttttacct gttaaatcga ggattctttc ccaaagtaac gttgccatct cttacgcata
     7141 cttgcagaca tgggctgagc cgcttctttg actgcgcagt atttgtccac gaatgtcaca
     7201 atatatcctt ctttatactt aggcggtgcg atcgttgcac cccacatgtg cttcaaaatg
     7261 atatctcgct caagatccga caaatcggtg attttttctg cattctttac agcaattctt
     7321 ggatggatat acgcatgtgt cccctcgtca aatttcgtcg tacgccaatc atagtagaac
     7381 agatcatgca aaagtccagc acgcgctgtt gctttagcat tgccacccca tttttttgcg
     7441 agcagataac tatagtaaga aacactgatg gaatgctcca aacgagtgga atgaacatgc
     7501 tgtgtgtaat tcgctaattt tttcactgct tctgtttcta aaagatcttg tacataagac
     7561 atatattctt cgtcttcacg ccatttttct gtcataatta tcgatttgcc acctttttga
     7621 atttcatttt cttctgaaat tgatctaagt ataccaccag tgttcggatt attctagtcg
     7681 ctctagataa aatagctaaa gcttaatagt tccttaatca taaagaaaaa tgataaagaa
     7741 cgatccctgc tgcgactccg acattcaatg attccgcatt tcctttcatt gggatatata
     7801 cgttctgatc agtcgaagac aagatttcct gacttactcc ctgaccttcg tttcccatga
     7861 ttatagcgaa atcctctgtt ttttctactt tgtctaatgc atgtgcttct tcatttaatt
     7921 ctgtaccgta caccgtaaac cctttgcctt tcattacagg gatcacttct gccaaatctt
     7981 tttgtaacac aggcaaatga taattgcttc cctgcatgct tcgcagcacc tttgtactat
     8041 aaatatctgc cgttccttta cctaataaaa caccagccat accaaacgca tctgccgtac
     8101 ggatcatcgt tcccacattt ccaggatcct gtacattatc tagtaaaagc cagcctcctg
     8161 aaaaatctgg cgtatcttca gatgttaacg tcatcactgc taagatccct tgcggtgtag
     8221 gtagatctga catagcggtc atgacctcat ctgaaactag aaaagtagaa atctctttgt
     8281 ttacagccag ccagtctccc cattctttca tacctctctg tgtcacaaaa agttctttta
     8341 tttctgcatc agacgacgca gcttcttcga tcagatggaa cccttccaaa agatattctg
     8401 ccttttcttc tctgtatttc tttttatgta gtttttttaa ttctttgatt cttggatttt
     8461 ttactgattg gatctctttc acattcctca cctcaactga ttatatcatg gaataggtgc
     8521 ttgagcttgt ttcatcctaa aaaaatcggt atcattgatt tataaaacag gaaagaaagg
     8581 tgacgaacat gcgcaaagtt aaaatgaatg tccaaggacg tgtccagggc gtgggatttc
     8641 gctatatgac caagatggtt gctgatcagt taggcgtgac cggaacggta aaaaacgaag
     8701 acgacggctc agtcacaatc gaagcaatcg gaaatgacga catcatccaa aaatttatcg
     8761 aagaagtcaa gaagtctccg agcccaagtg gacgagtgca gtatgtggat gtccaagaag
     8821 atccgatgat tgaggaacga aagaaatttg atgtggtggg ataaggttgt ttttgaagcg
     8881 ctttgcctcg agtattaagg agaaacattt tccaaatagc ttctcatatt ttgaaatgta
     8941 atgacttaat accgagaggc agctgataaa acaccgtcaa taaggttgtg aaaaagctgg
     9001 tctgcatcaa gcattaagga ctggttgtga aaaatagctc tccatatttt tcaacaggct
     9061 ggacttaatg ccgtagatgc aagcttttga acatcgtcga taaggttgcg aaaaagctga
     9121 tctgcatcaa gcattaagga ctggttgtga aaaagtcgct tagttttgag tattaaggaa
     9181 aattttgtga aaatggcttt ccacattttt gcataaattt gacttaatac caagaaactg
     9241 acttttgaac atcgtcgata aggttgtttt tgaggcactt tgcctcaagt attaaagaca
     9301 ggtagtaaaa aagctgatct ataacttgca ataaatttgc ttaacccata ttttccagac
     9361 agaacaaaaa agtttttagt tttttaaata agaaagaaac acttggaatc actcttttct
     9421 ttctaagtat ttctttcttg attttttgtc ttttttcaaa atttttcgct acgaaaaaga
     9481 acgaatattt atatgaaatt tctatgaagt tgattgaatt ccatgcgctt ttttagtata
     9541 gttaagtttg tgtaaaaatt atagaagagg aagtattcta tgaataaatg gaaaaactgg
     9601 ttattgggat ccggacttgt cagtatcctg ctctttttat ctggctgtgt gcggatggat
     9661 tcaaacggaa accctgacac ttcaggaatc gtttatcgag tactcgtcca tcctatgggc
     9721 caagcgatta catatctagt tgagaatttc aattggtctt acggctgggc agttattgca
     9781 atgactgtga tcgttcgtat catcatcttg ccactaggta tcagtcaatc gaaaaaaaca
     9841 atgatccaat ctgaaaaaat gcaagcgtta aaaccacaag ttgaagcagc acaacaaaag
     9901 ctaaaagctg caacaacacg tgaagaacaa atggcagcac aagcagaaat gcagcaagta
     9961 tatcgcgaaa atggattgag tatgaccggc ggaatcggct gtctgcctct attgatccaa
    10021 atgccgatct tctctgcctt gtattttacg gctcgctaca cagaaggtat ccgtgaatca
    10081 tctttttacg gcatcgatct aggctcacca agtatggtgc ttgtggccat cgcagggggc
    10141 gcttatctgc ttcaaggata tatttcaaca atcggtattc ctgaagaaca gaaaaagacg
    10201 atgcgtacga tgctgatcgt ctctcctgct atgatcgtct tcatgtcgat cagtgcgcca
    10261 gccggtgtta cactttactg ggtagtcggt ggtgtgttca gctgtctgca aacattcatc
    10321 acaaacgtta taatgaaacc gcgtttgaaa gcacaagtgg cagaagagtt gaaacaaaac
    10381 ccgccaaaac aagtcgttac tccaagaaaa gacgtgacag aatcagcaaa gccagctgac
    10441 aacaaacaag caacaaaaca aatcaaacaa ccaaaaaaca gtacaaacaa caaaggccgc
    10501 aacgcaggaa ggcagcagaa gcggaaataa aaatatatga caaaaaactg tagacaaata
    10561 aaaaaatttc tttgttctac agtttttttg ttacaattaa agaaaacgtt cgcatttcga
    10621 ggaggaatgt ataatgatgt ccaaataatc aatgtatagt cgaatggaag aagacaaaat
    10681 ttcgctagat gcacttgttc ctaaaaatca tttagtccgt aaaattgaca aattattgtc
    10741 atttgatttt atttatctca ttgttgaatc aacttactcg accattggtc gccctagcgt
    10801 tgatcctgtc gtcttagtaa aattagtatt cattcaatat ttatttggaa tttgttcgat
    10861 gcgtcagacc attaaataag ttgaaacaaa cctagcttat cgttggtttc ttgggcttag
    10921 tttaattgat aaagttcctc atttttcaac ttttggtaaa aactatgcac ggcgtttcag
    10981 agaaaccgat ttgtttgaac agatatttat cattattctc gaataagcag ttgatgctag
    11041 atttattcat gaagaaattt tgtatatgga ttcaactcat attaaagcga atgcgaacaa
    11101 acgtattttt acaagagata tggcacataa agaagccatt ttataccaaa aacaattaga
    11161 agaagaaatc aatgaggcaa gaattgccga aggcaaacgt ccttttggtt caatcattcg
    11221 tcaggaggtg gtcgaaagaa aatttagcat cgcagatcca gaaagtggtt attatgtaaa
    11281 aacagaacga gaaaaacagt ttgcttattc tgctcatacc gttggtgatg aaaatggttt
    11341 tgtattagat gtcatgatta cgcctggaaa tctacatgat agccgaatgc tcgtgccaca
    11401 aatcgaacgc gttaaatcac gttatggtgt agctgctaat gctgcgtaca aaacaccttg
    11461 gaacgtcaag tatttaatcg aacgaaaact acgacccatt ctcccttata tcagtttcgc
    11521 acaactactc gagatggcta taaaaaatat gtatctaaat cttttatttg tgaaagttgt
    11581 ccattattga aaaattgtac agaaagccaa aaacaccaaa aagaaataca tcgacatatt
    11641 tgggaagact acttagatga agcagaacat ttacgccata cggtgtacaa tcgtgaaaaa
    11701 tataaaaaaa gaaaagaaac gattgaacgc atttttgcag acgcaaagga aaaacatgga
    11761 atgcgctaca ccaagtatcg aggcttggaa aaaatcaggt gcatacaatg cttgtttttg
    11821 tcgcaatgaa tctgaaaaaa ttagctactt ggcagtggga gcatatgtcg ttatatcttt
    11881 tattatacaa aaatagacaa aagtatcagt ctacactctt agtaaagagg aaactgatgc
    11941 ttttgtctac agtcttagag agtgggacaa tagttttttc ttctttacgt cttttcctct
    12001 ccatgtattt tcctaatttt cttaatttaa cgccatgagt gccaagccaa tgtcaatctt
    12061 ggctccttgt ttccttcata agtggaaatc cgtttccgat agatcgaacc ttctttttcc
    12121 tctaaaagtt tttttacatt ctgctttgaa ataacgccat gtattattca ctaaaatttg
    12181 tcggtttcgc tcactttttg ctttcgtaca ttgactacga acaggtcagc accgaccact
    12241 ttcacattcg taaattttga agacacgtat aaatccactt ttatcttttc ttcgactaaa
    12301 gcgttgaccg caaaagcgac atctgtttca gaaatatatt gttccatatc taatggtaaa
    12361 attttttggt tgatggtata atttttaaac ataaagcact cgcttccttt ctgattgggt
    12421 ttggtcactt taatcctaca gggatgagtg ctttttgtgt accttaaaat cagtaaatat
    12481 ccatccgtga aaacgggtgg ctttttaaca gccctagaag ggctcaccac tcacagccct
    12541 atctctcttt aactatttaa atggatctac atactcttgt ttacttatac tgtctctaat
    12601 tcgatcatct gcttcttgtt ctcaaatata tatttcaatt gttttttgat ttaacccaac
    12661 cgtactcacg tagtatcctt tggtccaaaa gcttttattc ccatacttat atttcaaatt
    12721 tgcatgtttt tcatggatta aggttgcact tcgtcctttt aagtagccca taaaactaga
    12781 aatactaatc tttggcggta ttcttactaa catatgaatg tgatcaggca ttgcatgagc
    12841 ttcaatgatt tcaacatctt tcctctcaca caattttctt aatatctggc caatacttac
    12901 tcttaacttt ccataaatca cttttcgtcg atacttcggg gtaaagacta tgtgatactt
    12961 acaatcccat ctcgtatgtg ctaaactttt atcgctaaag ctatttcgtc cacgcgtaga
    13021 aacgcgcgtt tttttgtttc gtacgctacg ccaacgaaac tgactaaagt caataaaaaa
    13081 agtggggcaa taatcagatt tttttctgac tattgtccca ctctcttttt attactcttc
    13141 atcttctact attccactaa ttaattagtt gacagataca atgctttcac atcctgcacg
    13201 aacaaactcg ggctctgaac caatcacttg cgagttgata aattcaatga ccataggaag
    13261 attaatccga cataaagttt aatctgttgt ccttccatta tcagtttaga aatgatatta
    13321 gtaggtgtac cgcctaaaag atcgcatagt accacaaaat catttaacga atctatgaca
    13381 gtaagagctt ttgtctgtat gtttcagtgc catcttctgg taataaaggt aacgtgtaga
    13441 tattctcttg aggtcccata atcatttttg tacttttttt caattcctca caaaattttc
    13501 cgtgactaat caatatcaat tttctactca tttttggctc tcccttattt cgaaatcaca
    13561 cacaatgcag ataaagccac acacaagtaa tacgataaaa atcgctcgag ttgggtcata
    13621 tttttctttc ctaacaacca ataaacacct cgacgatccc agcgagtaag attttagata
    13681 atatcatatc gatatagttt tgcatattca atgtcacatc gccaatcgtt ggtaccataa
    13741 aaatttaata tcaatcattg ttgcaactaa tgctcctacc atgaacactc ttaataaagt
    13801 cgctacgtcc gttaatacag ttaagcgaga ttacactaga tgatctgttc ctgccaaggc
    13861 aagagccaga agcggttgcc tgcccatttg cctttggtgt gtcggagctg tccgataaag
    13921 ttcaccgccc gatccgctct ggctttgtcg tagtgacatt ccgaaagcat gaatcggctt
    13981 ggtgtgtatt cctttaattt gggatagccc tttggtctgg tttctgccat cagctaccac
    14041 ctcccaacag agcctccatc gcatcttctg ctccgacacc accgccatca ccggcaatga
    14101 tacggcttct ggacgaaggc gtcagaccga actgctcgga aaatcggttc ataattttca
    14161 gataggtctg ggcaataaac acctgtggta cctgctgcca atagccggat agggttttca
    14221 cgatgatacc gtgctgggta atgaattcct cggcttcctt ccagcgggcg taggcttggc
    14281 actaaccggc aaaggcagcc atatccacat ctgtcaagat tccgatggcc tccatctgct
    14341 ttgccagacg cttccattcc ttctttgcct ctggttccag ccacttcgga caggtcggtg
    14401 ccttcttttc cggcgttgac tcttttgcat tgagtgggcg attgcccgga tttccctcca
    14461 gcttcttcat ggctgtcggg gttgattttc ttcctctggt tgccacagtc gtcacctccc
    14521 ttcaatgtat ttctgccagt agacagagaa cagagccaag tcgaaacctg actctcttct
    14581 ctatctgcta acttgtattt cagtccactc ttctgatgaa tcgcacccgc aggattttca
    14641 agatgccgca ggcaaattcc aagaagtttc tgaccgcatc ataaccatcg tagccgtgta
    14701 gccacatcct tccacctcgc cgccgtcctc gtcgctgata taaatgattc tgtattcgct
    14761 catctgttgt tttctccagt cttttgtttt tttgtagtac atatatcact ctaaaagtga
    14821 ttattatcaa gtcgtttgaa agccataatg tacacaatca ttcgactggt ttttggttca
    14881 taattgaccg ccaaagtgag caaaagaaag ggacctctgt ctgtttcaga agtccctttc
    14941 ggtatttggt tcttagtggt tcaggctgta ttctcaaacc tcatactcat cttcgaggtc
    15001 tgcctctgca atgatctggc cgatgctgtc gtaacgctgg acctgcatct ggatggcatc
    15061 cttgtctgct ccctcgtcca tcatcttcag cagcttgtct gattcctttt tgtaggccca
    15121 gaaaaggatc tcctgaaggc tggtctcctg ctttttcttg cgaagggcga ccttcttctc
    15181 ttccaccgcc tcgttcaact tggtgaactc tgcccaagca aggataccgt ctcgcttgat
    15241 tgcgatctcc acatccttca gctccgggat ggtctttgca cccttgatct ccttcaaata
    15301 tttctttgcc ttttcgctca ttgccattag cgtttcctcc ttgtttttgg gattttcccc
    15361 tttggttgtg tgtatattgc catagtctct cgactatatc aagtcaatcc acaggcatat
    15421 actacacaat catttggagc tgtatttgta tacattatgc cgatttatga gagaagaaaa
    15481 ggctccagcc gttcgactgg aaccttgggg gggtcggttt cttaggcggc gtggcggtgt
    15541 agaccttgag gtagctgatg ttcttccaga cgtccgtgcc cttcagtctg accatgccgt
    15601 ccagctctcg gtcgagggtg aacttgcgga aaagcttgat gcggttgagg ctgtttgcta
    15661 gctccttgcc gttcttggca tccagaactg cgtcctcgat ctccgcaata ctaatttcct
    15721 gtctaaacca aaggttgctc aaggcttcgg gaatgccgtt tgcggtctgc agggggttga
    15781 aagtttccaa tacagcaggg gtcgaaacca cctgtccgag ctggaatttt gcgttcccta
    15841 aaatcatctg cataatttct tccatgtgaa tcatccattt ctgccgttgt ggtattccct
    15901 ttggcagtac agtactatat atcactctga atcgtacatt tatcaagaag cggatttttg
    15961 tggaatcacc aattcgatat aatagtaatt gcatgaaaaa tctaagcact ggattggtag
    16021 gtgatgatta tacgtgacga tcacatttac acctgtgatt cctgccacta cagcttccct
    16081 gccgacgaac agccagagcg ttgtcctgac tgcgaaaaga cagcaactcg gctggatacc
    16141 gaaatcgaaa ctgaagatta ctatcgtgtt cgtgctgaaa tcaaagctga gattaaagct
    16201 ttgaatgcgg gttaacgaca ggtactgacc ggcggctcaa caccgccggc tttttattac
    16261 cacatcttgc ggaattcgct gggacgaaca ccttccacct tgaaaaaagt tctggaaaaa
    16321 tagtttgcat ctgttatgcc aatttttgtt gcaatctctt ccatacttag atttgtaaag
    16381 cgtagcaagt gcttggcctg tgtgattcgt ttctgattca gataaccgac aacagttgta
    16441 ccgtaagttt cacgaaaaat cttagctaag tactgtttat tgataaaaaa ctctgcactc
    16501 aactgatcca gcgtaatctt ctccgtgtaa tggtcatcca gatattgttt tatgttctgc
    16561 aattccattt tttttgtaga gaaattcggc actttgtcag gattccaaga atcttccatc
    16621 aaatgcatca gcagttcact gagtagaaca ttgattttca tatcccggac atactcctgt
    16681 ccctctgcca ccatatgaag gctcctcagc aagtcagcat acttatctgt ttttgatgtt
    16741 ttaaagaccg gttgaccacc tcgttcaagg tattttttgt agacttccgt cataccaatg
    16801 ccattaaaat ggcaccaccg caaagtccac aaatcatctt ctgatgactc atggacatac
    16861 ggcttcaaac agtcaataaa caccatatct cctacttgaa gcgtatattt ctgcccctta
    16921 tactccaatg tgcctgaacc aacttctacc aggaaacaaa ggtaagatga caatcctgta
    16981 cgcctagatg tatgtgcctt cagagcagtc agctgtccaa tttcctgcaa atacaaaaga
    17041 gaggatcttg caaagaacga tgccgtgtac agcacacgat ttgacttcac cgatttcaaa
    17101 gcgttagaaa acagttcctc cattacaaga tttctccttt cttttcttac tcgaacattt
    17161 ccgccagttc agcagccata atagcttcca tagcttcggc ggcttccttg ttctctgcgc
    17221 tgacgctgat ataggtcttc agcttcggct cagtaccaga aggacgaacc acaacagagc
    17281 agttatcttc cagcataaac ttcaaaacat cagacttcgg cagaccattc aggcccttac
    17341 tgtagtctaa agtttcaata accttcttac caccaattat atcgagaccc ttgtggaatt
    17401 caaccataat ttcctgcatc ttggtaaaac ctgccgcacc gtcaaactcg aaggaatgca
    17461 gggtgttcaa acaatagcca tactttgtat aaatctcgtt cagcttatcc agcaggctga
    17521 taccctgagt cttgtagtaa gagaacattt cacagattag ataagcagcg ttaacaccgt
    17581 ccttgtcacg gacatagcta ccggacagat agccgtaaga ttcctcaaag ccgaagatat
    17641 agctgtcaac cttaccagcc ttttccaaca taccaatctg ctcaccgata aacttaaagc
    17701 cggtcagcac atcgatggta cgaacaccat agttggatgc gatacgttca cccaaatcca
    17761 tggtgacgat ggtcttgacc atcacagggt cagcaggcat ttttccatgc ttaattctct
    17821 gagaacagat gtagtccaga agtagcagac cagtctcatt accagacaac agaacatagt
    17881 caccatgtgc gctcttaact gcgataccaa cacggtcgca gtcagggtca gtggccaaca
    17941 gcagatccgc attgtacttc ttggcatact ccatacccag agacatagct tccttaattt
    18001 cagggttcgg ataaggacaa gttgggaaat taccgtccgg cagacgctgt tcttccacaa
    18061 cggtcacatt gctgtatccc atctctgcca gggtgcgggt aacaggcacc agaccggtac
    18121 cattcagagg agaatacacg atagcaacat tcttgtccac ttcttcgcca aacaggacgg
    18181 actgcccctt cacttcttcg atgaaagcag tcagaacttc cgactgaatg gatgcaatct
    18241 tagaagcatc gccatccatc tttacatcag caaagatgtc cagcttttcg atttctgcca
    18301 gaattgcagt agcagcctcg gtagtaatct gacagccgtc tgcaccgtat accttgtagc
    18361 cgttatattt agaagggtta tgagatgccg taatcataac accggcatct gccttcagat
    18421 aacgagtcgc aaaagatact gtaggaacag gaagcagttc tggccacaag tgcacctgaa
    18481 taccatttgc agcaaagatg tctgcggcag tctttgcaaa cacatcagat ttgatacggg
    18541 aatcatagcc aaccaccacg gaagcggctt catagttctt attcagatag tctgccagac
    18601 cttggcttgc cttacctacg gtgtgtacgt tcatgcgatt agtgcctgca ccgatggtac
    18661 cacgcagacc accagtaccg aaagacaaat ttttatagaa agagtcctca atgtccttct
    18721 cagaatagga acgcagttct tctataatat ctgcattggc atgattcagc caacgctcat
    18781 attctacttt ataattcata ttattatcct ctcacatcca gaaactgaat cgacccgtga
    18841 atgctgattt cagtagaatc ggccggcaca aaaaggcagt cccccttata gatgtccaac
    18901 gattcattat caaatcgaac ggtaccacag ccattgacac acagcaacac acggaagctg
    18961 attgaatccg cacgatagtg taccgtctgt cttctctcag tgttgacaat catacgagag
    19021 acctcgaaat gcttgcatct gcaaagcaat tccgcagcta caccagcacg atattttaga
    19081 acacgaagcg gctgtttcgg ttctgacaac cctttcaagt tcgctacttc cagcgctttg
    19141 tctacatgaa gttctctctt tttaccattt ttgtcaacac ggtcataatc gtagagacga
    19201 taagtcagat tggaattttc ctgaatttct gcaattagtg cacccgcacc aatcgcatga
    19261 atcgttcccg cctcaataaa gaacacatca ttctttttga ccggaacctt ctgaagataa
    19321 cgctcaacag ttccctgagc aatcgcttca cggatcacag cttcttctgt tattttctgc
    19381 agcccataga ttaagctggc atccagtaca taccacatct ctgtctttcc aagctgcccg
    19441 ttttcttttt caattgcata ttcatctgtg ggatgtacct gaacagacaa atcctttttc
    19501 gcatcaatga atttgatcag gatcggcaat tctcctttgg tctgcggatg cgttcccaga
    19561 aactctgggt gttcttgcaa cacctccgtc aaggtcatcc cctgatactc tgaccagaca
    19621 caacaaagct cggtccatcc gggtgcgtag agcattccaa gtttccgcaa gaggagacat
    19681 ctcaattcct tttgcaaatt catcattcag gcggttcccc cccacagata atcctttgcg
    19741 gaaggcttca ataaaaacgg tttctgtata acgagccctc ctccgttttt taatactcta
    19801 actcaaattt ctgtttatct ttaacattga tatccttgcc aagctgtact tcaatcagct
    19861 tcagctcagt atctgccata atggtatgcc gacagccagc actcatggtg atcacatcgc
    19921 cagccttaac aacctgctca tatccatcca cgatggtctt accagtaccg gccagaacca
    19981 cccacacctc gtcacggttc ttgtggctgt gataattcat ggagtgacta gcattcagag
    20041 taaccttaat ggtcatgctc tctgcttcta catccataac cttataacta ccccaagact
    20101 tctctgcgaa catgatctgc tgctcgaact tgtcaacgaa aggcttaata tagctggact
    20161 gttccttatc ggaaaccaga ataccttccc gagatgcaca gatgaccaca tcatgcagac
    20221 ccatagccaa cacaggaaca tccagttcgt tcaggatgtg tacgccatcg caattctcat
    20281 tcataacacc tttaccgata atggattcct ccataccttc ggtcagagta ttccaagtcc
    20341 ccagatcctt ccacatacca gcaaaacgct gtacctgaat cttgtcctca tgctcaacaa
    20401 cggcatagtc gaaggaaatc ttagtcagag tatcgtattt gttgaacaga tcctgataat
    20461 ctacaaagtc aatcagctga tgtgcacgct ccatgacata cttcagcttg tagacaaaca
    20521 caccaccgtt ccacagtgca ccctgtgcga tatattcctt tgccacagcc tcagaaggtt
    20581 tttccttgaa ggtatttacg aaagcagtct gctctgcgct ctcaggaatg atgtagccgt
    20641 acttctcgga aggataagtc ggttcaatac ccatgagcac cagattagct tcgcctttgt
    20701 cagcctgatc ggacagagat ttcagggcct caaaatagtc atcttctacg taagggtcaa
    20761 ccgggcaaac aactacggac tcttctggat caatgcccat cacatctacc agataagcgg
    20821 tagccaatgc gattgccggg aaggtatcgc ggcgacaagg ctctacagat acacccacat
    20881 cctcaccaag ttggttatgg atagcagaaa cctgtgtctt agaggttgca atcgtaacag
    20941 ttgcgtcgtt atctaccttc ttaatctggc ggtagacacg ctgaaccatg gattcatatc
    21001 catcttcagt tttgaaaatc ttaatgaact gcttggaacg gatgtcatta gacagcggcc
    21061 aaaggcgctt accagaaccg ccagacagta aaacaatatt cataatgatc tctccttttt
    21121 ataagaaaga ggctgctgca atcctcaaaa atcacagcaa cctcggtaaa ttagcgttca
    21181 gcacgcatcg ggttattcag gaagtcctcg taggacagac ggatgccatc ttccaactca
    21241 gtcttatatg tccatcccag cttggttgcc ttggaaacat ccagcagctt acgaggtgta
    21301 ccgttcggct tgctggtatc ccacagaatc tcgccttcgt aaccaataac ctttgctacc
    21361 atctcggtca gttccttgat agacagttcc ttgccagtac ctgcattaac agtctcgtca
    21421 ccgctatagt tattcatcag gaacacacac aggtttgcca gatcgtcaac atataggaat
    21481 tcgcgcagag gagaaccatc accccagcag gtaacagtag gcagacctgc ttccttagcc
    21541 tcatggaagc gacgaatcaa agcggggagc acatgactgt gagtaggatg atagttgtcg
    21601 ttaggtccat acaggttagt aggcataaca gagatatagt cagtaccgta ctgcttgttt
    21661 agaaactcac agtacttcag gccagaaatc tttgccagtg cataagcctc gttggtcttt
    21721 tccagctcac ttgtcagcag acagctttcc ggcatcggct gcggtgccat acgcggatag
    21781 atacaggaag aaccgaggaa ctgtaacttc ttacagccat tctcccatgc ggagttgatg
    21841 acgttcattt ccagaatcat gttttcatac atgaagtctg ccagagcgtt ctggtttgcg
    21901 ataataccgc caacctttgc agcagcgaga aatacataat ccggcttttc ctgtgcgaaa
    21961 aatgcttcaa cagcgtcctg acggcacagg tcaagctcct tatgggtgcg ggtgataata
    22021 ttgttgtagt cctgacggtt cagctcgcgc acgattgcgg aaccagccat gccacgatga
    22081 ccggctacgt agatttttgc gtctttttcc atcataataa tgcttgctcc tttctaaatt
    22141 tagagcatct ctcattgcat gagagaaatc aatttgtctg cgatttcgct cgcagcatca
    22201 taaaatgtat caaaatttga ggtgtctagc tgtttgaggt cctcaatcac ccgctctacg
    22261 cggtggaggt tttccggtgt gacggaaatt gccagaccca gtttttgcaa tttacaaata
    22321 gttgttctat cttccaaaac tgtatcgcgg gcgaacagtg ccatcggacg ctgtgcgaga
    22381 atacactccg caacagttcc ccatccagcc tttgtaataa cgtaatctgc cgccgctata
    22441 tagtcctgtg tattgggggt gctgttagaa ataacggtta cgttttctcc cacaagcgga
    22501 acacctagcg ttgtaatgaa atcatacggc gcgtgactga catcaatcgg ctgtttgaac
    22561 cgcgcggaca taccgagtgc gacaaataca ggcggttttt catgctgtga tttaatgcgt
    22621 tgcacttcgt cgttatgaat cggacgcgcc acaacggaag tcggtgtttt ttcgctgttt
    22681 tggagaaatt ccaacatttc ttcgttattt agcgcataaa gcatggtgtg ctgaatttta
    22741 ccgtaacgct tggcatattc tttccaaata tgttcgggta aaaattcgcg atacagctct
    22801 gtccaagtga agtttcctac atacaggaaa ggtactcctg ccatatcgca ggcatccatt
    22861 gcccagattg gcatatcgca aagcactgcg ctgacagctt ctcgcttgag ccattcggct
    22921 tctgtctttg ccaaagaagg aacctttgcc aaaaaatatt cacagatctc ggtcagctta
    22981 cccgtgtcca ccagtagggt gccctcacgg ttaatcagac cgatatctgt gtttattgga
    23041 cgatagcgca cgcggtctat cagctctttg ggcagttcct gctccaaatt gcgctttgca
    23101 aaatccagat gatttgcgcc gcaaaccagt gtaatcttcg cttcgcatcg gcagagtgtt
    23161 gccataataa tgggcagatt ccgcgcgata tgtccgaagc cgtgactgga aagatagaaa
    23221 cagatatgtt tattcaatgt cagcttactt gactgcgttc ttcattgcag cttcgcgctt
    23281 tgccaattca cggtcatgct ttgccatgat gttaaccagt tcctcgtaag aggtcttctg
    23341 cggattccaa ccgagaacgg tctttgcctt agtcgggtcg ccccacaggt tgtcaacgtc
    23401 agtcggacgg aaccactgtg gatttacaca aacgagcatc ttgccggttt caacatcgta
    23461 gcccttttca tcaatgccag tgccttccca acgaatggtc atgccatttg ctgcgaatgc
    23521 cttctcggtg aagtcacgaa cagtgtgctg ctcgccggta gcgatcacga agtcctcggg
    23581 agtctcatgc tggagaatca tccacatgca ctcaacatag tcctttgcat agccccagtc
    23641 acgcagggag tccatgttgc ccagttccag atggtcctgc aggccttctg caatgcggcc
    23701 ggcagcgagg gtgatcttac gggtgacgaa attctcaccg cgacgttcgg attcatggtt
    23761 gaacagaata ccgttgacag cgaacatgcc ataagcttca cgatattcct tagtgatcca
    23821 gaagccatac tgctttgcaa cagcataagg gctgtaagga tgaaacggag tggtttcacg
    23881 ctgaggaact tcttcaacct tgccatacag ctcagaagta gatgcctgat aaatcttagt
    23941 cttcttcgtc aggcccagaa tacggcaagc ttccagaata cgcataacgc ccaaagcatc
    24001 aacatcgccg gagtactcag gaacatcaaa agaaacctga acatggctct gtgcagcgag
    24061 gttatagatt tcatcaggct gaacaatgct gatgatacga atcagagaag atgagtcgga
    24121 caggtcaccg tcatgaaggg tgatcttgcc catcaaatgg tcaatgcgag cggtattgga
    24181 aatagaagca cggcgagtga taccgtgaac ttcgtagccc ttctctagca ggaaatctgc
    24241 cagataggaa ccgtcctgac cagtaatacc agtaatcagc gcttttttca tgatacatta
    24301 tcctttccta tgtaagtgat aaggccatat aaatggctcg taatttctaa tacttttcaa
    24361 gaatacacga taattttcga tatttcatag aacaatctac gcacgcatta gcacaatatt
    24421 agtttttcgt gaattggaat ggcaacagtt tttttgacaa attttataag gtgcagaact
    24481 tctttccgta tgctattccg attgtccttg acaatgagcc tcctcggatg tgacgatctt
    24541 cggcaggagc taacagttaa agttagactg cctcgctagc gttagcacat ccgagctcat
    24601 tatcaaggat tcgctgcgcc aatctctggt tacggtaacc aaccggtgtc tcgtagccaa
    24661 gtgt
//
LOCUS       DACDUU010000039        24289 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.039, whole genome shotgun sequence.
ACCESSION   DACDUU010000039 DACDUU010000000
VERSION     DACDUU010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.039"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            73..759
                     /gene="gpmA"
                     /locus_tag="IWL30_001607"
     CDS             73..759
                     /gene="gpmA"
                     /locus_tag="IWL30_001607"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP9706364.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(1057..1764)
                     /gene="deoD"
                     /locus_tag="IWL30_001608"
     CDS             complement(1057..1764)
                     /gene="deoD"
                     /locus_tag="IWL30_001608"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP9706365.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(1781..2599)
                     /locus_tag="IWL30_001609"
     CDS             complement(1781..2599)
                     /locus_tag="IWL30_001609"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP9706366.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            complement(2623..3789)
                     /gene="deoB"
                     /locus_tag="IWL30_001610"
     CDS             complement(2623..3789)
                     /gene="deoB"
                     /locus_tag="IWL30_001610"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP9706367.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            complement(3888..4844)
                     /locus_tag="IWL30_001611"
     CDS             complement(3888..4844)
                     /locus_tag="IWL30_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9706368.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            complement(4844..5977)
                     /locus_tag="IWL30_001612"
     CDS             complement(4844..5977)
                     /locus_tag="IWL30_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9706369.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            complement(5970..7532)
                     /locus_tag="IWL30_001613"
     CDS             complement(5970..7532)
                     /locus_tag="IWL30_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9706370.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            complement(7744..8826)
                     /locus_tag="IWL30_001614"
     CDS             complement(7744..8826)
                     /locus_tag="IWL30_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9706371.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            complement(8892..9290)
                     /locus_tag="IWL30_001615"
     CDS             complement(8892..9290)
                     /locus_tag="IWL30_001615"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP9706372.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            complement(9311..9973)
                     /gene="deoC"
                     /locus_tag="IWL30_001616"
     CDS             complement(9311..9973)
                     /gene="deoC"
                     /locus_tag="IWL30_001616"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP9706373.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            complement(10019..11320)
                     /locus_tag="IWL30_001617"
     CDS             complement(10019..11320)
                     /locus_tag="IWL30_001617"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAP9706374.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            complement(11461..12477)
                     /locus_tag="IWL30_001618"
     CDS             complement(11461..12477)
                     /locus_tag="IWL30_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9706375.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            complement(12785..13390)
                     /locus_tag="IWL30_001619"
     CDS             complement(12785..13390)
                     /locus_tag="IWL30_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9706376.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            13586..14509
                     /locus_tag="IWL30_001620"
     CDS             13586..14509
                     /locus_tag="IWL30_001620"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP9706377.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            14568..16133
                     /gene="guaA"
                     /locus_tag="IWL30_001621"
     CDS             14568..16133
                     /gene="guaA"
                     /locus_tag="IWL30_001621"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP9706378.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(16624..18255)
                     /locus_tag="IWL30_001622"
     CDS             complement(16624..18255)
                     /locus_tag="IWL30_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAP9706379.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(18269..19942)
                     /locus_tag="IWL30_001623"
     CDS             complement(18269..19942)
                     /locus_tag="IWL30_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9706380.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(19955..20272)
                     /locus_tag="IWL30_001624"
     CDS             complement(19955..20272)
                     /locus_tag="IWL30_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9706381.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(20297..21697)
                     /gene="lacG"
                     /locus_tag="IWL30_001625"
     CDS             complement(20297..21697)
                     /gene="lacG"
                     /locus_tag="IWL30_001625"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP9706382.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            21934..22719
                     /locus_tag="IWL30_001626"
     CDS             21934..22719
                     /locus_tag="IWL30_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9706383.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            22722..23414
                     /locus_tag="IWL30_001627"
     CDS             22722..23414
                     /locus_tag="IWL30_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9706384.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(23688..24059)
                     /locus_tag="IWL30_001628"
     CDS             complement(23688..24059)
                     /locus_tag="IWL30_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP9706385.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
BASE COUNT         7061 a         5402 c         4382 g         7444 t
ORIGIN      
        1 ccaaaaaatt gtacaataaa acagaactga aaatttcaga aatataaacc tatcttaaaa
       61 ggagagctaa acatgccaaa attagttttt tctcgtcacg gtcttagtga atggaatgca
      121 ttgaaccaat ttactggttg ggctgatgta aatctagcac cagaaggaat cgaagaagca
      181 aaagaaggcg gacgcaaaat caaagaagct ggaatcgaat ttgatgttgc ttacacttct
      241 gttttaactc gtgcaatcaa aacatgtaac ttgatcttag aatattcaga tcaactttgg
      301 gtaccacaaa tcaaatcatg gcgcttaaac gaacgtcact atggtaaatt acaaggtttg
      361 aacaaaaaag aaacagctga aaaatacggc gacgaacaag tgcacatctg gcgtcgttct
      421 tacgatactc ttcctccatt aatggaagaa actgacgaag gttctgcagc aaatgaccgt
      481 cgttacgcaa tgttagacaa acgcgatatt cctggtggag aaaacttgaa agtgacatta
      541 gaacgcgctt tgccattctg gcaagatgaa attgctccag cattactaga caacaaaact
      601 gttttagtag ctgcacatgg taactcatta cgcgccctag ctaaacatat cgaaggtatc
      661 tctgacgaag acatcatgga tcttgaaatt ccaacaggtc aaccacttgt ttatgaatta
      721 aatgatgact taacagttgc taaaaaatac tacctataaa aaacactata aaaaagggac
      781 tgtgacacga tttgtcacag tccctttttt cgtataaacg gttttctcct gccaacttct
      841 cggtcacaag tcacaatatt catcaaacat gaaaagctgt tttttgaaat tgttccttgt
      901 gactcagagt taaacggcag attcacaacc tcttggtaaa cggtgttcaa aagcttacat
      961 ttgcaataat aagtccagcc aagtcaaaaa catgaggagc tatttttgct tggatgttct
     1021 tattacttaa tgcagggcag ctttttcatt tccttcttat tctgctatcg ctgtttccaa
     1081 tcctacttcg atcatttcat tgaacgtaga ttgacgttct gcagctgttg tttcttcacc
     1141 ggtgatcaaa tggtcgctaa cggtcatcaa acttaatgtc tgcacatggt atttcgcgcc
     1201 taagtaataa agagccgctg cttccatttc cactcctaaa acgccaagtt cagctaattg
     1261 gaacgtctct gtcaaatcat ctttgtagaa agaatcttcc gaaagaacat ttcctacatg
     1321 aaccgtatag cccttttctt tagctatctc atatgctttc agtaatagat tgaaatcact
     1381 gattggagga aaatgaaagg cttggaaatt tttttcgatc atcgaagaac tggtcgctgc
     1441 accttgtgct agtactaaat cacgaacatg gacgtctttt gacaatgcac cgcatgtacc
     1501 tactcgaatc aatttcttta caccatacga ctggatcaat tcatgggcat agatggtggc
     1561 tgatggcatt cccattcctg ttccttgaac agaaatacgt tctcctttat atttgccggt
     1621 aaagcctaac ataccgcgta cctgattgta acaaactgga tcttccaaaa aagtctccgc
     1681 aatatattta gctcgcaaag gatctcccgg aagtaaaatc ttatcggcga tctcgccttc
     1741 tttcgcttca atatgcacac tcattttatt gttcctccta ttacaataat gtcaatgttt
     1801 ctttaaccaa tgctttaaaa gattgtttga ctctctgagt tgtttctaca acttcttcat
     1861 ggctcagatt cgcttgcata cctgctgcta agttggtaat acaagagatt cctaacactt
     1921 ttaatccgct gtgagctgct acgatgactt ctggaacagt cgacatccct actgcatcgg
     1981 ctcctattgt ccgagacatt cggatctccg caggcgtttc ataagttggt ccagaatagc
     2041 ccatatatac gccttcttta agatccaaac ctagtttttc tgctgctgct tttgctgttg
     2101 cttgatagtc aggcgtataa gcttgactca tatctggaaa tctagggccc attgcatctt
     2161 cattcggacc gatcaaagga ttttcaccag taaaattgat atgatcggta atcagcatca
     2221 aatctcctgg tacaaatgat tcgttgacac caccacatgc attagtcaca atgactgaat
     2281 gggcccctag tccggccatc acacgtactg gataggtcac tgtttgcatg gaatgacctt
     2341 catagtagtg gaatctccct tgcatggcta acactttctt gcctgccaga gacccataaa
     2401 ctagctttcc tgcatgccca actactgtcg aaacaggaaa gaacgggatc tcatcataag
     2461 aaatcattac agcatcttcg atttcatctg ctaattcacc taatcctgaa cctagaatca
     2521 aaccaaaatc aatttctttt actcctttat caaggataaa ctgggtcgtt tcttgtaaca
     2581 tctcgcttaa cttggtcatt tgctttctcc tatttaaaaa gattatttta ataatttcaa
     2641 gaagctttcg ccattttcag tcgaagaaac actaaagttc tcagcaatcg ttgctgaaat
     2701 atctgaataa tatccttgag gcaatgcccc ttgttctttc atttttttac tgtaaaccaa
     2761 taacggtaca tattctcgcg tatgatcggt accagtaaac gtcggatcgt ttccgtgatc
     2821 ggctgtaatc attagcaagt cgtcttcttc catcgcttca agcaattctg gaattcgtaa
     2881 gtcaaattct tcaatcgcat gcgcatagcc gactacatcc cgacgatgac cgtataatgc
     2941 atcaaaatct actaaattgg taaagctgat tcctgaaaaa tcgtttttca taactttcag
     3001 cagctggtca acgccatcca tattgctttt cgtgcggaca gattcagtga ttccttgtcc
     3061 gttgaaaata tcgttgatct ttccaacagc aatcacgtcc tttcctgctt ctttcaaaga
     3121 atcaaggact gtgtgtccga atggattcaa cgcataatca tggcgattgc ttgttcgtgt
     3181 gaagtctcct ggttcaccca cgtacggacg tgcaatgatt cggccaatca tatacggatc
     3241 atcttttgtt atatcacgaa cgaattggca aatccgatat aattcttcta aaggaatgat
     3301 ttcttcatgt gccgcgatct gaaggacagg atcggcagaa gtatatacaa ttaaatcgcc
     3361 tgttttcatt tggtgttcac cgtaatcgtc aataacagct gttccgctat aaggtttgtt
     3421 acatacgact ttacggccag aaaattcttc gatttgtttg atcagatcat cagggaatcc
     3481 attaggaaac acacgaaatg gagttttgat attcaatccc atgatttccc agtggcctgt
     3541 catcgtatct ttccctacag aaacttcttc taatttcgtc gcatagcctt gatgatcttt
     3601 cactgattcc acaccttcaa gaggacggat cgttcctaaa ccaagctgct ctaaatgtgg
     3661 aacgttcaat cctgcttctt tcgcaatgtg tcctaatgta tcagaaccaa catcaccgaa
     3721 cttatcggca tctggtgctt ctccaattcc tactgaatcc atgacgatca gatgtacgcg
     3781 tttaaacatg attattcctt ctttcttttt atttttattg cctttaaaac aaaaagggcg
     3841 agacgaaaaa cacttcccgt aacccaccct ttttattcgc taaaagatta ttttgatttg
     3901 atatagttct tcccgtttgc ttttggacca gaagctttcc ctaagaaaac aaccagcaca
     3961 atgattgtca gcaaatatgg agcaatttgc agcaatactg gcggaatcga agaaataact
     4021 ggtaattgtg cgccaatgat actcaagctt tgagcaaaac caaagaacaa tgaggcaccc
     4081 attgccccta aaggattcca cttaccaaag atcatagcag ccatggagat gaacccttgt
     4141 ccaacgatcg taccagctga aaagttaccg gaaatcgact gagcaaaaac agcaccgccc
     4201 ataccgccta acaagccaga aatcagtacg cctgaataac gcatagcata tacattgata
     4261 cctaatgtgt cggcagcttg aggattttca ccaactgaac ggaggcgcaa gccgaatttc
     4321 gtcttgaaga tgatcaccca agctaggaca gcaatcaaaa tagcacaata agcaggtaag
     4381 aatgtatttt taaagaaaat tggtccaatg accggaatat ctgctaatcc tgggaaagaa
     4441 aaatacccca gattctgcgt gatgttatca gtttgacctt taccatacca tgctttgatc
     4501 aagaaaacac ctaaagcagg tgccatcaga ttaattactg tcccgctgat aatatggtca
     4561 gcacgcaaac tgactgttgc tactgcgtga atcagagaaa ataggatacc tacgacgcca
     4621 cccactaaaa tagcgatcca tggtgtcata gaaccaagtg atggactcat cgtcaagttg
     4681 aaaacgatgg aactaaaagc acccatgacc atgattccct ctagccctac gtttacgatc
     4741 ccgcttcgtt cagaaaaagt tccgcctagt gacgttagaa ttagcggtgt tgaataaacc
     4801 agtgtagaag taaggatcaa ggcaattaat tcgatagtcg acattatagt tcgccccctt
     4861 catggttttg attcgttggt ttcgattcta ttgtcgcaac gatctcttct tgttttttcc
     4921 cttggaagac tttagccatg acgaaacgaa tcaggaagtt gatcccgaca aagaagataa
     4981 tcaaagcaat cgatacattg acgatctcaa acggaacacc tgctcctgtc tgcatgccaa
     5041 gtccaccgat cttcaaaata ctgaacaaga tcgctgataa aagaataccg attgctgatc
     5101 cagaaccaag caaggaaaca gccattccgt caaatccaat agatagcgaa gtcgtttgaa
     5161 caaagaagtt ttgatacgtt cctaatcctt caaccacacc gccaagccct gccaaagcgc
     5221 cagaaatgac catcgacaaa acgatcgtac gtttactact cattcctgca tactcagaag
     5281 caaaaggatt caatccaaca gaacggattt caaatcctaa agtcgttttc ttcatcagca
     5341 gccaaacgaa aataagcgcg atgattgcta aaaataatcc tgagtttacg cgagagccgc
     5401 caaaagcttg tgttaaccat ggtaaccgca gtgaggcatt ttgactgatc atattggaag
     5461 agtccaaact tgagcgatag ctttcaggca tcacttcatg taaagcaaat gtgcttacgt
     5521 ataataagat atagttcatc atgattgtta cgatcacttc acttgttcca aaaaatgctc
     5581 gaagtaaacc tggaacagcc gctgccatcg caccagctaa ggcacctaag atcacgcata
     5641 acggaagcaa aacaactttt ggcagatctg gaaacgcaag tgccacccag acactgacga
     5701 tccacccgca aagtgcttgt ccagaaagcc caatattgaa aaacccagct gagttagcta
     5761 cagaaaaacc taatgctgta aaaatcaacg gcgttgcttg ccttaacgtt tcacctatcc
     5821 ctctttgcga accaagggct gcatctagca tagaagaata accttggatg ggctgaaaac
     5881 caaaagcagc catcaatatc gcgcctaaga tcaatcctaa aacaactgaa agtacaggca
     5941 ctaataaatt acggaatttt tcgctgcgat cattcatgtg cgccagcccc cttttctaac
     6001 tcttttcgtg cttcttctaa ggtatagccc gccatcaata gacctaattc attttcagat
     6061 gtttctttcg gatcaacgat accgacaatt tctccttcat ggataacagc aatacgatcc
     6121 gaaacattta aaatttcttc taattcgaaa ctgatcagta acactgcttt gtatttgtcc
     6181 ctttgttcga tcagacgttt atgaataaat tcgatcgcac ctacatccag tccacgagtt
     6241 ggatttgcaa caatcaacag atcaggatct cggtcgactt cacgagcgat aattgctttt
     6301 tgctgatttc cgcctgataa agcttttgcg ggaacaagtt cattcgttgt tcgaacatca
     6361 tattcttcga tcaattctct ggcatgttca ttcattactg aataattcag catgccgttt
     6421 ttactgtaag gtttttgatg ataggtttgt agtgcgatat tttcagataa tgtcatatct
     6481 aggactaacc cgtatttatg acggtcttct ggcacgtgtc ctacaccatg ttcagtgatt
     6541 tttcggggtt ttttattagt gatgtcttcc ccttttagct tgatatgtcc gctttctgcc
     6601 tttcgcaaac cagtcaaagc ttggatcaac tccgactgcc cgtttccatc gattccagcg
     6661 ataccaagta cttcgccagc acgaacctct aagttcaagt ttttcacggc ttctaatcca
     6721 cgattttctt tcaccactag attttcaata gacaaaacga cttcttgagg cttcgcttct
     6781 tttttcatcg ttttgaatga aaccgttctt ccaaccatca tatcggctaa ttgttgtgag
     6841 gttacgtctt taacgttgac tgtaccgatt ccttttccac ggcggataac tgtacagcgg
     6901 tcagctactg ctttgatttc atctaactta tgcgtaatca aaatgatcga cttgccttct
     6961 ttgactaatt ccttcatgat cacgattaat tcatcaattt cttgaggggt caatacagct
     7021 gtcggctcat caaaaatcag tacatctgct cctcggtaaa gtgtttttaa aatttctacc
     7081 cgttgttcca ttccaactga aatatcacga acgtaagcat ctgggttgac tgacaatcca
     7141 tactgttcag aaactttttg gatctctttt cgtgcttttt tatggtcaag catccctgcg
     7201 cgactaggct cgcttcccaa cacgatgttt tctgttacag taaaggcatc aacaagcata
     7261 aagtgctgat ggaccatccc aatccctaat tggttggctt tggtcggact tgtgatcttt
     7321 acttctttcc cacgcatcaa aattttccct gatgtcggct ctaataatcc ggaaagcacg
     7381 ttcatcaatg tagatttgcc agcaccgttt tctccaagca acgcatgaat ttctcctgcc
     7441 tttacttgta aattgatgtt gtcatttgcc ttgaatgttc caaattcttt ggtaatattc
     7501 tgcatttcta tgacaatatt ttcttgaacc accttttttt cacatcctaa cgatttctat
     7561 ctagggcaac gattgactaa gattcacgct tagtgagcgt tcttcttttg aaacaaacat
     7621 tacttcaggg agttgttccc ttatccaaac tgtcacaagg cagcttggat aaatgaacaa
     7681 ttcttgttct aatcaaacca tagccttgtc aaacatgtgt ctaacaaggc tatagaaatg
     7741 gttttagttt tcttcaggag tttctggaac ttttacgtca cctgaaatga ttttttcttt
     7801 tgcttcatca actgcttttt tcgcttcgtc agaaagttgt ccttctgtta gaccaacacc
     7861 gtcttctttc aatccataaa cagtgtgttc gccgccaggg aatttaccgt ccgctgattt
     7921 ttgtgccaaa tcttctacta ctgttccaac tgctttcagt gtagatgtca atgtgaagtt
     7981 tttcttttcg ccgtttaatg tgtattcgcc ttcgtctgat tggtcgcggt caacaccgat
     8041 aacccaaact tttttgtcgc ctgattcgtt caatgatttt gcttcttgga atacaccgtt
     8101 acctgtaccg cctgaagcat ggaagatgat atctgcgttt tgtgcataca taccttgagc
     8161 aattgaacgt cctttatctg gagcagagaa gtcaccagca tattgattca atactttgat
     8221 ttcttttcct aattctttcg cacctgcatc aacaccggct ttgaatccag catcaaaacg
     8281 atcgattact tcacctttta caccgccgat aaagccgact acgtttgttt cagtcgtata
     8341 agctgctgca acacctgcaa gataagaagc ttcgttgtct ttgaaagttg cagatacaac
     8401 gttatctaat ccatcgatga catcatcgat aataacaaag ttcgtacctg tattgtttgt
     8461 tgcttgttct tggatcgctg gttttagttt ataaccaata ccaaaaatcg ttttgaatcc
     8521 agcattcaat gcttggtcaa tatttgggat gtaatctgat tcatttgaag attggaaata
     8581 ttggaagcca tcatttcctc gtgaaagtcc ttgatctttc ccccattttt ctagtccttc
     8641 ccaagctgac tggttgaatg aacggtcgtc tacaccacct gtgtctgtga tcaatgctgc
     8701 agttgttgtg tctgatgagc ttttgccacc ggatgaatca gtagagccgt tgttgccgca
     8761 tgcacctaaa aataaccctg ctgctagtgc gacagcacct aaaccaaata attttgcttt
     8821 tttcatttct gtaaagcctc ctaaaataat tgttttattt ttcaacagtc agctgactga
     8881 atgaacatga gttatagatc tttttcagta aatgcataag gcagcagtgc acgaacggtc
     8941 gttgccttcg tcacaccatt gtctcctact agcgtaaccg gcatatcagg ggaacagaat
     9001 tctgccatta cttgtcgaca tgcaccacaa ggagagatag gatcgggggt gtgccccgct
     9061 acgactagat gcgtaaatgt tttttctcct tcagatactg ctttgaaaaa agctgtgcgc
     9121 tcggcacaat tcgttaatcc aaatgatgca ttctcaatgt tgatgccttg ataaatcttg
     9181 cctgattccg ttacgagaca agcgcctacc ggaaaatggg aataaggaac ataggcttta
     9241 tctaacgctt caatagctgt gttgatccat tcttgtttga gttctgccat gagcttctcc
     9301 tttgaaaaaa ttaatagcct tcaccagttg aaccagatac gattgctact cctgcgcttg
     9361 ttccgattct tgtagcacca gcttcgatca ttgccattgc ttcttcttcg ttatggatac
     9421 cgccggaagc tttgacaccc atttctggtc ctacagtttc acgcatcaaa cggacatcct
     9481 ctacctttgc tccgcctgtt gagaaaccag ttgatgtttt gacaaaatca gcgcctgcag
     9541 cttttgctat ttcgcaagct cggatgattt cttctctatc taataacgat gtttcaataa
     9601 taactttgac aagcgcacga tcttttgctg cttcaacaac tgcttcgata tctttttgga
     9661 cttgttgatc atcctttgat ttcaaagcac cgatattgat gaccatatcg acttcatctg
     9721 caccgttatt gatggcatct gttgtttcaa atgcttttgt ctcgggtgtg tttgctccta
     9781 gaggaaaccc gattactgta catacggcta ctggctcacc ttgtaattgt tctttcgcta
     9841 gcgcaaccca tgctggattg atgcagacag aataaaaatg gtattttttt gcttcttcga
     9901 taatttgcat cacagcttct ttagatgcgt ctgctttcaa aatagtatgg tcgatcattc
     9961 ggtttagttc catttctttt ccccttttct ttaaaacggt aaagtccgct tttttccttt
    10021 attctgtgat gatgtcatga atcaacgttg gcttcattgc cgattcacca atttcaatat
    10081 ttttatataa taattcaatg acagaagtga tttcttcgtc gttactatag atcgtcaata
    10141 gtgactcacc ttttgtcaca gtgtcaccaa tttttttatg cagtttcaat ccaacagcgt
    10201 gatcaatatc ttcttctttg gttttacgcc cagcacctag catcattgca gcaattccta
    10261 attcatttgc cacgattttt gacacaactc cgctttgttt agcaggaagc tccattgtgt
    10321 agcgtgcagt caaaattcgt tctggatgtt ccacgatcgc tgggtctccc ccttgatttt
    10381 ggatcatttc tttgaatttt gctaacgctt tacctgattc cagagcttct tgaagcaacg
    10441 ttcttgcttc atcggttgtc ttagctttgc cagcaagaac aaccatttgg cttcctaagg
    10501 cgtagcacat ttctactaga tcttctggac catttccttg taatgtttcg attgcctcga
    10561 caacttctag actatttccg atagcttcgc caagaggttc tgacatatct gagataaccg
    10621 ccactgtttc tcggttagcc agtttcccaa tgctcgtcat ggtctttgcc agacgtcttg
    10681 catcttcgat gttcttcatg aaagcaccgt ctccagtggt cacatccaaa acgattgcat
    10741 ctgctcctgc cgcgattttt ttactcataa tcgaactggc aatcagcgga agagaatcaa
    10801 cggtcgctgt gacgtcacgc aaggcataca attttttgtc cgccggtgcc aagtttcctg
    10861 attgaccaat taccgctact ttactttcat taacaatgcg tacaaattct tcatccgaaa
    10921 gctcgatctg aaatccggga attgcttcta gcttgtctag tgtaccacct gtgtaaccta
    10981 gaccgcgtcc agacatttta gccactgtca ctccgacact agctactagg ggggctaaaa
    11041 tcaatgtggt cgtgtcacca acgccacctg tcgaatgctt atctaccttt attccttcaa
    11101 gagaactcaa atcgatgaca tcaccagaat tagcaatcgc caatgttaag ttcgtgattt
    11161 cttcatttgt catgtcttga taaaaaattg ccatcaataa tgcactgact tgataatctg
    11221 gaatctttcc attcgtatag ttcgtgacga tgtactcgat ttcctctttc gacagctcgt
    11281 ttccatccct ctttttttca atcagatcca ccattcgcat tgcctgatcc tccttgtctt
    11341 cattcggtat tatacattat ttttaggaaa ggtaaaccac tttagtaaac tggtttacta
    11401 aaaataaaaa aagcactaac tagcctttgt aaatgcttac ataagcaata taccgccgag
    11461 ttagcccttt gtcaatactt ttacgctctc acgaataatt aattttgttt cgaacacttt
    11521 gttcggaact tttctttgag gaaattcgat cgtatcgatc aggaaacgtg cagcataaaa
    11581 tccaatatca aaacttggct gctgcactgt cgtcaattgc ggaatcatgt attctgacag
    11641 ctttaatcca tcgaatccta tgactgagat atcttctggg atttttttgc ccaattcatc
    11701 gatcgcctga tagcatccga tcgccatggc atcattcccg caaaaaatag ccgtgatatc
    11761 tttatgtttg agtaattttt ttgatgcagt atatccacct tggacagtca actctccatg
    11821 ttcaacaaat ttcccattaa aaggcaggcc atgatctttc agcgcatgac gatatccgtt
    11881 gaaacgttct tctaattgat agtatccagt ggtttccttc agcatgccga tcttcgtatg
    11941 gccattttca ataaacagcg aaacagcttg ataggcgccc tcatattcct ttacgataag
    12001 ccgtccgtta tcccgacgat tcaatccgcg atcgattaaa atgattggca ttttttgata
    12061 aaaaccgctg tctaatgcat atttctcagg taaaatattt ggtgtggcca gcaagattcc
    12121 gtccactgag cggtgggaaa gctcttgtaa atacttgatc tcattttctt ggctatgacg
    12181 agaattgcat aacatgatca catagcccaa aggattcaaa tagctctcta caccttcgat
    12241 cagtttggag aagaagaaat cagtgacatc agggacgatc attccgatgg tctttgaatg
    12301 tcgattgatc acatttgaag cgaaataatc gggcttataa tgatgttcat tcacgatcga
    12361 caacactttt tttcttgtct tatcactaaa tctgcttcct ttgttattca aaatctgtga
    12421 gacagttgtt actgatacgc ctgccatttc tgcgatatct ctgatggtca gcttcatcct
    12481 attggctccc ttctatctaa attccctatc agtgtaccaa aacccccaag aaaagaatag
    12541 agaaaaaagg aaagtatgtt ttttgataaa aacctccttt atctaccgac ttggcagcct
    12601 tctatacaag ttctgtatct tatttatttt atcgtaaaaa acaacgactc tgttcctact
    12661 atttcttttt gaagaaaaaa tagttctttg tttttaaaaa cttctcaaca aaaaaagaac
    12721 tgatacgtac ccgtacatgc acgcccagtt ccactctttc gtcgctccac tatttttcag
    12781 ctagttattc tttcacactt ttgatgatat aatatccttt atcttttgtg acgatttctg
    12841 cattaccaaa tgtttcttcc attttctttt gcgcactcgg cgctccttgt ttcttctgga
    12901 tcacaattgt cagtgtaccg cccttcttca accgaggata tgcgcctgtc aaaatttcat
    12961 gtacaacttt ttttccagca cggatcggcg gattgcttac gatagctgca taggtttctt
    13021 catgaactgc ttcatagata ttcgattgat ggatatcaac atgttcgatt tggttacgct
    13081 ttgcgtttcc ttgcgctaga cttaccgctc gttcattaat gtcgaccatt tcaacaaacc
    13141 gctgtgtcgc aaatgccaaa gctaacccaa tcggtccata cccgcatccc acatctaaga
    13201 tctttccttc atcaggtaac tcttcccaat cgaaagcatc aatcaggaca cgtgaaccat
    13261 aatccactgt ttcacgtgaa aatacaccag aatctgttac aaattgaaag gttctgcctt
    13321 ttaactcaaa agcccattgt tcaaaatcgt gcgccgtatc tgggcgttca gaatagtaat
    13381 gatttgtcat gtcttccatc cttctcattt ctttcatgac aacagtctat ccttttatgt
    13441 atccgtgcgc aagtcaagaa taagactccg tttctttcta ttataaagat tatacaaaaa
    13501 gatcggcaat ctctcttaag tggtggggtt tgcgtttatt ttgtgttaaa atagttccat
    13561 tgatgggaga acgcaggagg ctaatatgaa tgatccaatg aactactacc gtgtggcaag
    13621 agaagaatgg cgagaatttt atcgtaatgg gcaagcacct ttgacacaag atgaactaga
    13681 taatatcaaa agtttgaatg accgcatttc catgcaggat gttcgagata tctatgtacc
    13741 tcttgctcat ctgatttatc tttatatgaa agaatttgaa tctttgttct tgagtaaagg
    13801 tttatttctt cataaatacg tagaagtacc gccttttatt atcgggatcg ccggaagtgt
    13861 agccgttgga aaaagtacaa cagccagact gctgcagacg attttgtctc gtaccttcaa
    13921 acgaagaaac gttcagctga tcacgactga cggatttctt tatccaaatc gtatattaaa
    13981 agagcgtgga atcatggacc gtaaaggatt tcctgaaagt tatgatatgg agatgctgat
    14041 cgatttcttg aaccgtgtaa aatctggtca ggaagagata aaagtacctg tttactcaca
    14101 tgatgtgtat gatatcattc cagacaagta cgaattgatc cagcagcctg atatcttgat
    14161 cgttgagggg atcaatacgc ttcaattacc tgcgaatcag caaatctacg tcagcgactt
    14221 ttttgatttt tctgtctatg tcgatgccga tcccgtgctg atcgaaaaat ggtatttgga
    14281 tcgttttggt gcgttactgg atacggcttt ccaaaatcca gagaattatt attatcgttt
    14341 tgctatcgga gatcgtgaag aagcctttaa aatggcaaaa gatgtatgga aaaatgtgaa
    14401 tttgaaaaat ttacaggaat atatcttgcc tacacgagga agagccgata taattctcca
    14461 taaaactgaa aatcatatca tcgattcgat tttcatgcga aaatattagc attttaaaat
    14521 agtttgacta ttttgagata aaatttaaag ggataggggt aataaatttg acaaacgttg
    14581 ccgatatgac gaacgttgaa aagattatcg tattagacta cggtagtcag tataaccaat
    14641 tgatcacaag acgtattcgt gatcttggag tcttttccga attattgagc caccgcatta
    14701 ctgcagaaga agtaaaagcg atgaatccaa agggaatcat tctttctggc ggaccaaaca
    14761 gtgtatatga tgagaatgca tttgggatcg atcctgagat cttcaattta ggtattcctg
    14821 tattagggat ttgctatggt atgcaattga tcacgtacaa acttggcggt tccgtagagc
    14881 ctgctaaaaa ccgcgaatat gggaaagcct tgttagaagt aacaagcgat caagctgtct
    14941 tgtttaaaga aacaccaaaa caacaaacag tatggatgag ccatggtgac ttggtaacta
    15001 aagttcctga aggctttgaa acagtcggaa caagcaaaga ttgtccgatt gcttctatcc
    15061 aagacacaac acgtaatttt tatggtatcc aattccatgc agaagtacgt cattccgaat
    15121 acggaatcga attgctgcgc cactttgcct ttgatgtttg tcactgtgaa ggtaactgga
    15181 caatggataa ctttatcgat atgcaagtag aaaaaatccg tgaacaagtt ggtgacaaaa
    15241 aagtcttgct tggtctttca ggtggcgttg attctagtgt cgttggggtt cttttacaaa
    15301 aagcgatcgg tgaccaattg acatctatct ttgttgacca cggtctgcta cgcaaaggcg
    15361 aagcggaaca agtaatggaa agtctaggcg gaaaattcgg cttgaacatc atcaaagtca
    15421 atgcaagaga tcgcttctta gacaagttga aaggtgtatc tgacccagaa caaaaacgta
    15481 aaatcattgg gaatgaattc gtatatgttt ttgatgacga agcaacaaaa cttgctggtg
    15541 aagaaggcgt ctctttccta gcacaaggaa cgttgtatac tgacgttatc gaatctggta
    15601 cagaaacagc acagacgatc aaatctcacc ataatgtagg tggactgcca gaagatatgc
    15661 aatttgaatt gatcgaacca ttaaacacat tattcaaaga cgaagtccgt gaattaggga
    15721 ctcagttagg catgcctgat tcaatcgttt ggcgtcagcc attccctggt cctggtcttg
    15781 gtattcgtgt tcttggcgaa atcactgaag acaagctaga aatcgtacgc gaatctgatg
    15841 ctatcttgcg tgaagaaatc gcagctgctg gacttgaccg tgatatctgg caatatttca
    15901 ctgttcttcc agggatccgc tctgtcggtg ttatgggtga cggacgtact tacgactaca
    15961 cagtcggtat ccgtgctgta acttcaatcg acggaatgac cgctgacttt gcccgtatcc
    16021 catgggaagt cttgcaaaaa atctctgtac gtatcgtaaa cgaagtagcc cacgtcaacc
    16081 gtatcgtgta tgacatcact tctaaaccac cagcaacagt ggagtgggaa taaagagcca
    16141 acataatatg gagggggaat ccctataaat caacattctt caatagctaa aatgcttgat
    16201 tttcattaaa aataagatgg ttccctacca aaagagacta taatataagt taatttgttt
    16261 cattcttatc ataaaaagaa gttctcgaaa aacgaactgt tttccgagaa cttttttcta
    16321 tccatctaag tgctcaaatc atattcacga cggaattcta aggttaccct ttcttaaaca
    16381 aactttatgt tatctgcaca actttttgca attttttcat tgcttcttac gaaatcgttg
    16441 aactctcttt agcataatac aaagtgtaga tgaagtctgg tgctttactc gttttggtaa
    16501 aaatgactgg aaatcgtttc tggttatttt tggcaaaggc gaggtgcagg ttcgattcct
    16561 gccttctacg ctaacgatgt ggctgaaggg aacctccgat cttgaatcga aacgatccgc
    16621 agtctattgt ttcaccaatg aaacaaaatc aacgttcgtc caagcatttc ctgaagcctt
    16681 gctataaacc ccaatttcac aaacacctgt tgtaattgga atgtctttga ttgtaatttt
    16741 tgtccattca cttgttttaa caaccgcagt atttagttcc gctccaccat aatttttgat
    16801 atataactga ttagtatcaa actctccccc agacattaca taggcactca atgtgtaagt
    16861 tcctttgctt atgccactaa cattttgata agctgatgcc tcgtatgctt gatcctgcca
    16921 aaaagttagt ttgtagtccc caaaaaaagt gcccgcttcc gttttgatcg tacccttagc
    16981 ggttccttgt tgaaaccagg tttgccaatt ggaaggactg gtcgttggtc catcgctttc
    17041 gaaacttgca tttttaacta gatttatttg cgcccctgct tgtttaaacg cacgcaagct
    17101 tggcaaggcg tttccagcaa aatcaaacat cgcttggttg tcccattcgt ttcccatagt
    17161 ggattgaaca ctgagataat tcatacctgc ttgtgttgcc caactcacat ttccgttgta
    17221 ccaaagaggt tcccaataga agaagcccgc cccgcgatta ttcggtactg cctgaatccg
    17281 atcggcaaga tccagcaaat actctgcttg tcctgtcggt gttggttcat atccccccgc
    17341 agctgcttcg gcttcaccaa aagcatttgg catagtatcg gcattcgctg ttgtatgccc
    17401 ataagctgtt tcaactacga tcacttcttt accataccgt tggctgatcg cattcatatt
    17461 ggtttgaagt tcattcattg aaccgtgcca atacggataa tacgacactc caattaaatc
    17521 aaatgacaca cctcgactcg tgatttcatc aaaccaccaa gtaaatgctc ctgtatcgcc
    17581 accatcagct aagtggagca ttactggagt tgttttaggt tgagtgtctc gaacagcttg
    17641 gataccagac ttcaaaaaag cagccaatcg atcaaattct cccccgcctt ctccccaaga
    17701 cttgccataa ggccagagca ttccgctatt cagttcattg ccgatctgaa ccatatcagg
    17761 ataaacgtcc agctgcttca tttccgacag aacgttcttt gtatagtcgt acaatgccgt
    17821 attcatttct tcaaaggtca gaccttgcca tgctttagga agattctgtt tcccaggatc
    17881 aacccaaaaa tcactgtagt gaaaatccag cagcacctta agtcccagat tttttgctcg
    17941 ttttgcaagg tcaatcgtcg tttccagatc atttgtccct gctccataac tttctccatt
    18001 actattataa ggatcctgcc acaaacgcag tcgaacatag ttgacaccat tattttttaa
    18061 aatctctaag gcatcttttg aaatatcgtc ttcataatat tttgcaccag atttttccat
    18121 atctgacagg atcgaaatat ctgctccgcg aataaattcg ctggcagatg cttcaacagt
    18181 ttttgaaaca gcccccaaag cccctaacac agctgcaaat aaaacagctt tacgcaacca
    18241 aattcccatt ttcattatgg ctcctccttt attgaaattg gttcagaata aactgaacag
    18301 ctttttcagg atctctcgtt aatccgatat attctgctcc ttttgtcgca accagcttga
    18361 ttcctaagcg atcggtgtct tctttgatat catcatagta agaattgact tgcggtgcta
    18421 agaccaccat atcaaaatcc tgcagcattt cgtaatgaga accataagca cccgccattg
    18481 cactgatcgg tgctttcgat tcagatgcac ctttttttaa agcattagct aacatcgcac
    18541 ttgtccctgc gccagcacaa agtaccaaca cattgatttc tttatttgca tcgctgataa
    18601 cttgactcgc tgccacaggt acttcctcac gtacatcggc agaagcacct tcctcggttg
    18661 cgatcacctc tgctttttgt gcttctttct ctactaagac agcatcgtat gctttgcaaa
    18721 atggtagata gatcaggaag tcaacgacga ttagaaccgc tgctagaaca aaagctaaga
    18781 tacccatgcc cgttcctaga accaaaccga tcggtgctgg agttgcccaa ggcaaaacgt
    18841 acataaagct gttcatgccc aatacatcga caaaaaactt gaacaggcaa acgtttacaa
    18901 caggtgttaa caaaaatgga ataaaaaaat aagggttcaa aatcatgggt gccgcaaata
    18961 gcaatggttc gttgacagcg aaacttactg gaataaagga agctttccca actgccttca
    19021 actgcttaga cttagcaaag agtaggaaga taaaaggaac aaccaatgtt gccccagttc
    19081 ctcccatagt tcctacaaaa ttccctaaac ccaccgtcag tactttagaa gcttgttcac
    19141 cggaattgaa caattgcaga ttggtttcca cattggcata gataattgcc gcaattgccg
    19201 gctccacgat cgacggtcca tgaacaccga caaaccaaaa cagcgccatc gctccccaga
    19261 taatagcgat tcctaagtac ccgtcagctg ctgtaaatag cggctgcagc accgtaatga
    19321 ttgcttctgc aaatgagatt tggaagattt gtcgaatgcc taaatcaatt aaaacggccg
    19381 caaatactga aaaagagaaa ggaaaaatat ctcggaaggt ttgcgaaatc gttccaggaa
    19441 cttctttagg cattttaatg gtaatatctt tcagcacaca aaacttatat aaattaactg
    19501 ttaaaaatgc agcgacaaag gaagccagta gtccctttgt ccccatatat tccgttgaaa
    19561 atccaccttc aatcggagag acgcttaaca acaggaaact taccacagag gccagcatca
    19621 cagaggtggt gttgattact ttgtttttcg gtaagcgacg attcatcgaa tcagccaagc
    19681 agcgagcagt ggttgctgaa acgagtaacc ctacaacacc catcgaataa ttatagactt
    19741 tccatagcca tgtggagaca gattcaggcc attggtaacc aaaaatctca ggaaccgcag
    19801 caatcaaaat gaaaatgctt gaaaataaaa tagctggcat cgctgttaaa aatccgtcac
    19861 gaacggctcc taaataacta ttacgtgaga ttttttcaaa aaatggtttc cctttttcga
    19921 tttgtttaat gattccatcc atagcgttct tctcctatcc ttgattctgt ctgtacagta
    19981 caataaagtc ttgaatcaga tctctcagca ataaagtggt cattaaatga tcctgcccat
    20041 ggataaaaat gaatcccatc tccatctctt tcccagatgc ttcttccgct aaaatttttg
    20101 tttgcgaatt atgtgcaagg gttaaatttt cgtcggcttc tttcaacgcc gaatcaacat
    20161 gttcaaagtt tccttgtctt acttctttca ataatttcaa taatgtactg cgagcgtcgc
    20221 ctgaatatgc aacgatctca aaaccaagca tttgtaattc ctctttattc atcttttctt
    20281 cctccattat cacttattaa ataatcgttt tggtttcact gaccaacttg taccagtaag
    20341 ctgattcttt tgggtaacgc ttctgggttt caaaatcaac ataaaacaag ccataccttt
    20401 tgttataccc atttgtccat gagaaaagat ccataagcga ccacaaaaaa taccccttca
    20461 catttacccc ggctgttatc gccttgctta aggattccaa atagactctc aaatagtcga
    20521 ttctcggctg atccataata atgccatctt caaactgatc tttatacccc atcccgttct
    20581 cagtgatata gattttgttg taatgtgggt aatcgctttt aattcgcagc aacaagtcat
    20641 atagaccttc tggataaatc agccagtccc aatcagttcg aggaattcct tctttataaa
    20701 tacgttctcc gattcccttc actttataaa cagaggtccc tttttcaccc gtaccgttat
    20761 gatggattgc attttctcca tcgtaagctt tgacaaaatg gcattggtag tgattgatcc
    20821 ctaagtagtc attacgcgtg gatgcttttt tcaattcaac gaaatcttct tcaggaaaat
    20881 gatagctggc ctgatttgct tcacatatct catcaagagc agtcaaggtt tcagttgaat
    20941 aatagcctaa ataggtagca tctaataaga aacggatcga caatgcatcg tctaaaaagg
    21001 cagcatgctt atcttccggc gcgtctgttg ccgcatattt cgtctctaat gagtgaacta
    21061 caccaatttc acccggaagt tcgttttctt tgaaatagtt cactacacgg gcatgagcaa
    21121 ccatcatatt atgaagacag gcgacgatct ttgtaaaatc atatttgatt cctggtggga
    21181 aaacacctaa caaatattga ttggttgcaa ctggatagat ttcattaaag gtactccaca
    21241 ccttgacttc tttgaattca tgaaaacaaa aaatggcata ggaaacaaat gcttctattg
    21301 tttctcgatt caaaaaatct ccatggtcaa acaaacgttt aggggtatca aaatgatgca
    21361 acgtcacaaa cggcgttacg tttcttttct tacattcttc gaacacacga tgataaaaag
    21421 cgattccttc tggatttggt ttcccagctc catcaggaaa aatgcggctc caagcaatgg
    21481 ataatcttag accatttaca ccaaaacgtt cacacaattc aatgtccttt gcatattgat
    21541 gataaaaatc acttgccgga tcgggactga atcgcccttg ttcctctaaa aagtcatccc
    21601 aagcaacagc gcctttgcca ccttccttag tggctccttc aacctgatag gctgctgtgg
    21661 ctccgccaaa aataaaatct tctggtaatt taagcatgta catctctcct caaatctata
    21721 atcagttttc gctctttcta actgattgca ttgtaaacgc atccaaaaca tttgtcaaca
    21781 aaaacaaaca caaataatca taaaaaccag aaaacagcaa taaaatctat tgtaacaaac
    21841 ataaaaacca ccctaaatgt ttacttttgt tcgaattaga ttgactgttg tccgtttata
    21901 tgatagatta tccttaaatt agaggtgaag acaatgttga aaaaagaaag attgcttact
    21961 atcgtagaaa tggtcaataa aaaaggaatc cttaccgtta atgaaatcat caatcaatta
    22021 gatgtttctg atatgaccgt tcgcagagat ttagatgaat tagaaaaatc gggcaagctg
    22081 ctgcgtgtcc atggaggggc tcaaagtctt tcctacactt tagatcaaga actttctcac
    22141 actgaaaaag ccactctgca gattcaagaa aaaaaagaaa ttgctgccac agcagcctcc
    22201 ttgattcacg agggagaaac gattttctta ggaccaggta caacaatcga actattggcg
    22261 acgcatttac tgaacaagaa aattcggatc attacaaata attatccagt ttttgatatt
    22321 cttgctcaag cagatggacc agaaattcta ctgattggcg gtgatttccg gaaaaatacc
    22381 ggcgcatttg tcggaccgat caccaacgac aatttaagaa gactaaagtt tacaaaagca
    22441 tttatcagtt caaatggcgt tcacaacgaa gcgatctcta cttacagtac cgaagaagga
    22501 gaagcacagc agatcgctct aaataattcc cgtaagaaat atctactcgt tgataataaa
    22561 aaattcaatc gagatgattt ttatgtgttt tataatttgc atgatttcga tctgctcatt
    22621 acggatcgcc agatctcgcg ggaggttcaa gagcactatg cacaatatgt cgatctgcaa
    22681 attgctgatt caaccgaaaa taccactata ggagcgtaac tatgaaaggg attatatttg
    22741 atttcaacgg aacaatgttt ttggactctc atctgcatga agctgcttgg ctgcatatga
    22801 tacatgatca tacaaaagat ggtttatcag acgaggatat tttaaaaaat attcatggtc
    22861 ggacgaatac ggagattttg aaacatttca tttcagagca tttgaccgcc tctgagatcg
    22921 ccgtattgtc agaagaaaaa gaaagctatt atcgtatgct ttgcttgcaa aatgaaaacg
    22981 agcttcagtt gacaaaaggg cttgaagcga ctcttgacca gctgactgac caacagattc
    23041 cattaacgat tgccacggct actgtcaaag agaatgttgc tttttatttt gatattttcg
    23101 atttagcccg ctggtttgac tgggacaaga ttgtctatga cgacggctct ttccctggga
    23161 aaccgcaacc ggatattttt cttaaagcgg cagacaaact gagcttagct ccggctgatt
    23221 gtcttgttat cgaagatgct tactcaggat tacttgctgc caaacgtgcg gggatcggaa
    23281 caattattgc aattgatcca ttcggtaaaa acaaaacagt atttgaacag gcacagctaa
    23341 ataaagatgg aaccatcaaa gattttactg gtttttggac agagcacctg tctgccacat
    23401 ccgttaattc ttaaacgatt caactaacta ccaaagaaaa gcaactgaaa tgcacacctc
    23461 cttttataaa aaactgccag tgaaacgaca tgaaagatac actgctatat ccttagtgaa
    23521 atatagcagt gtatctttga ctttttcatt cttttaacca tatgacgcgt tcatctctat
    23581 ctataattaa cattaaaaat agtgttttca cattatttct caaaccaatc ttaaaaatca
    23641 tcaggagtac ttttttgata tacttttatt aatgctgggc gtaatggtta aaagatttcc
    23701 actagttcct ctccaccaag ctgagttgaa aaatgcttat ctgtaaacaa catagacaaa
    23761 gaggaaataa acgtttccgt atctttctct gtcccaagga tattaattaa ttttcctact
    23821 aggtaagtat cattaaactt aagatctgcc ttcacatcaa aagttttgat aaaagctttt
    23881 atcatgcgta caattttgtc tttatcgcca taattgattt caccactcat atcaatatag
    23941 tcactaatcc ctgaagaaat aatggcaact gcttcttctt tcgccttatc agataattca
    24001 tttaatgact gggattgatt gatgacttct tgagttttat ctgttacgta atctctcatt
    24061 atcctatcgg catactccgc aaagtaatct tgaaaaaatt cttctacagg gtaaccaact
    24121 tcgttttccc aaccaggaga aatttcatta tcagtactta ttaaatctat taaaaaccaa
    24181 cccgttctct gagtgttgat tctataatat ttttaatttc ctatataaat caaaaatatt
    24241 atagaatcaa taagctaatt aagaaacaaa ctttagaaac tctagaaga
//
LOCUS       DACDUU010000040        23965 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.040, whole genome shotgun sequence.
ACCESSION   DACDUU010000040 DACDUU010000000
VERSION     DACDUU010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23965
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.040"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(78..647)
                     /gene="thiT"
                     /locus_tag="IWL30_001629"
     CDS             complement(78..647)
                     /gene="thiT"
                     /locus_tag="IWL30_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAP9706386.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(713..815)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(821..1792)
                     /locus_tag="IWL30_001630"
     CDS             complement(821..1792)
                     /locus_tag="IWL30_001630"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP9706387.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(1886..3259)
                     /gene="glmU"
                     /locus_tag="IWL30_001631"
     CDS             complement(1886..3259)
                     /gene="glmU"
                     /locus_tag="IWL30_001631"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAP9706388.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(3762..4580)
                     /gene="purR"
                     /locus_tag="IWL30_001632"
     CDS             complement(3762..4580)
                     /gene="purR"
                     /locus_tag="IWL30_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAP9706389.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(4747..5571)
                     /locus_tag="IWL30_001633"
     CDS             complement(4747..5571)
                     /locus_tag="IWL30_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP9706390.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(5544..6224)
                     /locus_tag="IWL30_001634"
     CDS             complement(5544..6224)
                     /locus_tag="IWL30_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP9706391.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(6247..7185)
                     /locus_tag="IWL30_001635"
     CDS             complement(6247..7185)
                     /locus_tag="IWL30_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAP9706392.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(7430..8281)
                     /locus_tag="IWL30_001636"
     CDS             complement(7430..8281)
                     /locus_tag="IWL30_001636"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAP9706393.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(8435..11008)
                     /gene="mprF"
                     /locus_tag="IWL30_001637"
     CDS             complement(8435..11008)
                     /gene="mprF"
                     /locus_tag="IWL30_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP9706394.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            11149..11955
                     /gene="proC"
                     /locus_tag="IWL30_001638"
     CDS             11149..11955
                     /gene="proC"
                     /locus_tag="IWL30_001638"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAP9706395.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(12034..13590)
                     /gene="rny"
                     /locus_tag="IWL30_001639"
     CDS             complement(12034..13590)
                     /gene="rny"
                     /locus_tag="IWL30_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAP9706396.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(14001..15050)
                     /gene="recA"
                     /locus_tag="IWL30_001640"
     CDS             complement(14001..15050)
                     /gene="recA"
                     /locus_tag="IWL30_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAP9706397.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(15159..16400)
                     /locus_tag="IWL30_001641"
     CDS             complement(15159..16400)
                     /locus_tag="IWL30_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAP9706398.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQFAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(16640..17218)
                     /gene="pgsA"
                     /locus_tag="IWL30_001642"
     CDS             complement(16640..17218)
                     /gene="pgsA"
                     /locus_tag="IWL30_001642"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAP9706399.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(17427..18311)
                     /locus_tag="IWL30_001643"
     CDS             complement(17427..18311)
                     /locus_tag="IWL30_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9706400.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(19089..20375)
                     /locus_tag="IWL30_001644"
     CDS             complement(19089..20375)
                     /locus_tag="IWL30_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP9706401.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(20368..21636)
                     /locus_tag="IWL30_001645"
     CDS             complement(20368..21636)
                     /locus_tag="IWL30_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP9706402.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            21888..22349
                     /gene="mscL"
                     /locus_tag="IWL30_001646"
     CDS             21888..22349
                     /gene="mscL"
                     /locus_tag="IWL30_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAP9706403.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(22408..22854)
                     /locus_tag="IWL30_001647"
     CDS             complement(22408..22854)
                     /locus_tag="IWL30_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9706404.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(22947..23192)
                     /locus_tag="IWL30_001648"
     CDS             complement(22947..23192)
                     /locus_tag="IWL30_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706405.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(23307..23885)
                     /locus_tag="IWL30_001649"
     CDS             complement(23307..23885)
                     /locus_tag="IWL30_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9706406.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
BASE COUNT         6830 a         5222 c         4155 g         7758 t
ORIGIN      
        1 tatttcttat caaaaaataa aaaggaaaca agaaactagg acatgattct gtctcaagtt
       61 tcttgcttct tttttattta cagctcctgt cgtttatctg gcacaaaaat cgaaggagtc
      121 gtttgttgga tccctaataa aacgatgatc gttacaatag ctgtactcaa cccgctggca
      181 ccattcatta cgaaagaaaa gagccaagga ttcatccccc atagtgcata cgctccccag
      241 aaaaccactc ctgcaataaa atgccaaaaa tatctggaaa gtgcgccaat caatgcacca
      301 aacacgatgc tttttctaat ttgtttcgta ttatcgtcta catagccttt ttggacattt
      361 gaagccatga gccctgctgc gcctgcaaaa gtataggcaa tcgggtactc aatcaacact
      421 tgaacgacac ttaaataata aacttgtccc aacggaaaat gtaggatgcc ccaaatgaaa
      481 cctgccagca tccctgcctt tgtccctctt cttaacgcaa aaagagtcaa tggaatctgc
      541 cctaatgaaa tagaaaaact gctgccgatc tggatcggaa tcaacgacaa caccatcgct
      601 aacgctgcaa caatcgttcc ttctacccaa actctatttt ttcccataaa aaaacttcct
      661 cctttgaata aaaaagtcac aaaggaggaa gcagctttcg aaaatagtca aacagctgtt
      721 tattcacaat tcctacgctc gcataaccga tacaggtacg aagggtttag aaatagattc
      781 aatctcagcc caattggact cccctttgtg agtttattta ttatttattt tttgtttcaa
      841 acaatggact aactggtttg ttttcatgga tacgtttgat tgcatctccc attagtccgc
      901 caacacttac ttcatcgatt ttgtcgattt tacgatcttc tggaagatag atcgagtctg
      961 taacaactag acgctcgata gctgaatctt ctattctttg taaagcaggg ccggataata
     1021 ctggatgtgt acatgaagca tacacactag ttgcaccagc ttctttcaaa gcattggcag
     1081 ccaatgtgat cgtacctgct gtatcgatca tatcatcgat cagtacacaa gttttgcctt
     1141 tgacttgtcc aatgatattc attacttctg caacatttgc ttttggacgg cgtttgtcta
     1201 tgatcgcaat cggagatttc aaaaattccg ctaatttacg tgcccgtgtg acaccaccat
     1261 ggtctggtga aacaacgaca acatcatcgc ctttgattcc tttttctaag aaataattgg
     1321 caataagtgg cgcacccatc aaatgatcga ctggaatatc aaagaaacct tggatttgga
     1381 ctgcatgaag gtccaaagtc agcattcttg ttgcaccagc tttttgaatc atgtttgcta
     1441 ctaatttcgc tgtgattggt tcccgtgcac gtgctttacg gtcttgacgc gcatagccat
     1501 aataaggcat gactacgttg attgtttttg cacttgctcg tttcagtgcg tcaatcatga
     1561 tcagcaactc catcaagtta tcattgactg ggctgctcgt tgattgaata acatagacat
     1621 gcgatccacg aatactttct tcaatgttta cttgaatttc tccatcacta aattgagtaa
     1681 cagatgattt tcctaactcg acgccgactg ctgctgcaat tttctctgct aatgggcgat
     1741 tggaatttaa cgcaaaaatt tttaatcttg ggtcaaagta atgttttgac atggggacct
     1801 ccactttctt tttcacaact ctagaattct acctaataaa tagtagtcat attttttaaa
     1861 taaatcaagt gattttgcaa aagtcttagt ttagataagg gagttttttt gcatatcctt
     1921 ctttattgac ttgtcttgct ctcgcaattg ccagcgcata ttctggaata ttgtctgtaa
     1981 ttgttgaccc ggcagcaatc gaactgtttt tagctacctc gacaggtccg atgatattcg
     2041 tgctagaacc aataaagctg tggtcgccga cagtcgtatg atgtttgttc tttccatcat
     2101 agttgacgaa gacaacacca catccgacat tgatttcttc tcccagcgta gcatcaccaa
     2161 cataagtcaa gtgaccgact ttagttcctt tgccgatttg agcatttttc acttccacaa
     2221 agtttccgat atggacgttt tcgccaattt cagcttttgg tcgtaaatgt gcataaggtc
     2281 ccacatctgc atgtttcttg acatggctgc tttcaatgac tgagttttca atgacgacgt
     2341 gatcctcaat cctactgtct acgatttttg aatgtgaccc gatgacacaa tcagaaccaa
     2401 tgactgtatt tccttggatt tgtacacctg cttcgatcaa tgtgtctgga ccgatttcta
     2461 cgcctgcatc gatgtatgtc gttgctgaat cgacaaagct tacgccattg accatatgca
     2521 tctggttaat tcgctttctc atgatctcat ttgctttagc taacgcgatc cgatcattga
     2581 ctcccattga ttcttcaaaa tcatccgttt gataagcagc aactgtatgg ccttcttctt
     2641 ttaagatttc gatgatatct gttagatagt attcaccttg agcattgttt gttccaactt
     2701 tgcttaacgc atcgaacaaa gcttcgttgt caaaacagta agttcctgta ttgatttctt
     2761 gtacaagagc ttcttctggt gttgcatctt tttgttccac gattttttct acgatcccaa
     2821 tatggtcacg gatgattcgt ccatatcctg ttgggttttc tgcttgtgcc gtcaagatcg
     2881 ttgcacttgc attttttcct tggtgatatt caaaaagatt gttcagcgtt tcagtagtca
     2941 ataatggtgt atcgccactg ataactaacg ttgttccttt ttttccttcc aagaaagaag
     3001 cagcttgcaa aacagcatgt cctgttccca attgttctgc ttgtaacgca tatttactgc
     3061 gttctcctag ttgtgcttta acttgttctg ctccgtgtcc aacgatcgta atcacttcat
     3121 ctggtttcgt ttcactgacc cgattaatga tatgttctac cataggttta ccagaaacgg
     3181 gatgtaaaac tttatataat tttgacttca tgcgggtacc tttccctgcc gccaaaataa
     3241 ttgcataacg tgcgtccaac tgcgtcactc ctaaatatag tttgctgatt tcagtttatc
     3301 ccagtatacc cattttttca acaaaacaag tgattttctt ttatcccagc gttttctgga
     3361 taaaaaaata gatagaccat aacgggccta tctatttggt aaccggtgct ctcctgccaa
     3421 ctcctcggtc acaagtcaca ctactcataa aacatgaaaa gctgtttttt gaaatcgttt
     3481 cttgtgactt agagttaaac ggtaggctta caacctcttc attaaacgtt tttcaatcag
     3541 ctgaccttcg gtattagctc aataaacggc aaaatatgag gagcaatttt tccgtttatt
     3601 gagctaatac tcaggacagg acgctgattt cacaacctct agataaacgg tgttcaaaag
     3661 ccagtttctc ggtattaagc caaatttatg aaaaaatgtg gaaagccatt ttcacaaaat
     3721 ttttcttact actcgaaact aagcggcttt ttcacaacct cttattctga aaaataattt
     3781 cctggcacaa ccgtaatatt tttcgtttga gtatctacat ctttgacata aagcagtgac
     3841 gtataatcat cgatggcgcg atgaccgttg aattttgatt cggcaaatac agtgatgcca
     3901 acaagttctg attcgaattc ttcaatcaag ctcttcattc cgttgactgt tccgccgcct
     3961 ttcatgaaat catccactac aaggacacgt gaaccacgtt tcaaacttct cttagacagt
     4021 tccatctttt caattctttc agaagatcca gacacatagt tgacacttac tgttgatcct
     4081 tctgttattt ttgagtcccg gcgtacaata acgaatggcg cattcagata ataggacaca
     4141 gcttgggcaa taggtacgcc ttttgtggct acagtcatta cagcatcgat tttttcacca
     4201 aggtatttag aagcaatgat tcttccaact tgacgcagca gttcaggttc tcctaacaaa
     4261 tcagagagat aaacatatcc tcctggaagc agacggtctt gttctgatag acgttctgct
     4321 agagattcaa tatatttttt cgcctcttca tatgaaattt cagggatgaa caatacacca
     4381 cctgcagcac ccgggattgt ttccaaggtt ccataccctc gttctttaaa tgttttcttg
     4441 atgattccca aatcttcact gatagaagat ttagcagatt cataacgttc ggcaaagctt
     4501 gttaaggaaa tcaattcatg cgggtgattc aataaatact gtgtcatgtc aactagacgt
     4561 tcactgcgac gtactttcac ctattccaac tccttttaag aaattttatc gttttacatt
     4621 atataagttt ttcttcaaaa attcgagcat tttatgaaaa aataagaaaa tagttcggta
     4681 tttattaaaa taaaaagaag cctataaaaa atttttcgtt tttataaggc ttcttttctt
     4741 tataatttag ttattttttc ttctgaaggc aaccaatatt cgtttgatga tatttacaat
     4801 caggaacaaa gcaataaaga ctaatgtgat tgttgctcct ggaggtgtat ctagataaaa
     4861 cgagctagtt agtcctgaca gcatccctag aaatcctaca caaacactga tagcaatcac
     4921 gacattgaag cttttgccta atcgcatacc gattgctgcg ggaagaacca tgattgcgga
     4981 tatcaaaagt gcaccggcaa tcgggatcat cacagcaata gccacaccag taatcacatt
     5041 gaacatcatc gacatcaaat gaacgggtag cccatccacg tgggcggtat cttcatcaaa
     5101 agtcagaaca tacatcggcc gtttgaataa aaagaacaat ataccgatgg caacgaataa
     5161 gattcctaaa aagatgactt gctcccatgt aatggtcacg atcgaaccaa acagatagga
     5221 ttggatactt gttgctgaat tccctccgct caaattcatc aatactaacg ctagcgccaa
     5281 accaccagcc attaaaatgg caatggagat ttctgaatag gttcggtaga tcgttcgcag
     5341 atactccaat atcacagctg ctaatacaac gactagcatg gtcgtaagtg taggattaag
     5401 gttcaaaaag aatcctaacg ctacccctgc taaagagaca tgagaaagcg tatcagccat
     5461 cagcgattgt cttctgatca ctaaaaacac acctagcatc ggggcgattc cggcaatgaa
     5521 gatagcggca agaaacgctc ttctcatgaa ttcatatgaa agcaacgcca ttgagaatcc
     5581 tcctttctca ccagtcggat ctgtctgtcc gcatactgtt tgatatcttc atgatcatgg
     5641 gtgatcatta gaactgattt cccatggaca tgcgcactat gtcggagaag acgataaaat
     5701 tcatttctgg attcttcatc catacctgtt gttggttcat caagaacaaa taagtcaggg
     5761 tcagtagcaa aaatacgcgc taaactgatt cgctgctttt gtccgccaga caattcacct
     5821 attcgccgat ttcgcatatc ccacatccct acagcttcta aagcacgctg tacatgttga
     5881 tgatcttttt cagagagacg cttgaaccag cggcctcttg gatatcttcc tgaacggact
     5941 aattcgatca ccgtactagg aaaacctgca ttgaaagatg caacttgttg aggaatataa
     6001 ccgatactta atttttttcc ttcttgattt ttctcagcaa tctttatcat tccttttgaa
     6061 ggtttcagta atcctaacga tgctttcacc aaggttgact tggctgctcc attttctcca
     6121 gttaaaatga caaattcacc cgcatctaca tgataagaga catcttctag tacaggttca
     6181 tcatcatagt aaaaggtcaa atcagatatt tcgatataac gcaccttaca acctccattc
     6241 taaaacttaa ttgatacttt cttttaaagc tttcagattt ttttccatga cagaaatata
     6301 attttcgcct gctttgattt gttcattagt caagctttct aaaggattca acacttctag
     6361 cttcactcct gtttcattcg acaatgtctc agctaccttc gaagaggcat tttcttcaaa
     6421 ataaatcact ttgatgttgt ttttcttcac aaattctttt agctcagcta agcgacttgg
     6481 tgtgggttcc tgatctggtg aaacgcccga aatcgctact tgttttaagt cgtattgatt
     6541 cgctaaataa gcaaaagcag catgctgagt gacaaatgtg cggtttgttg catcttttag
     6601 ctcatcggag tatttttgat ccagtgcttc aagctttttg atataagcat cggcattctt
     6661 cgtaaaagtt tcctttttat ctggatattt ttcgcctaat tcttcagcaa tcgttctgac
     6721 ttcttttata gctaataccg gatcgagcca aacatgtgga tcgtattcat gtgaatgacc
     6781 atgcccttct tctgattctt catgttcgtc tgcttcttgc gattctaaac ggtcgattcc
     6841 tttagctgct tcgatcatca aggtctgttt gctatccact gaatccttta acgaatccac
     6901 aaaaaattcc atgtctgaac tattatacac gaaaacatct gcatcggaaa tttctgctct
     6961 ttcttttgca cttggttcga aatcatgcgg ctccgttcca gcagggatca aaagtttcac
     7021 gtttccttca tcccctacga tctcttttgt aaaatcatac atcggataaa aagtcgtaac
     7081 gatcgtcaaa tcctcttttt tgtctgcttc cttgttcgta tttccacatg ctgcaaacag
     7141 taaagcacct atcattaccg tagctgtcaa aagatatttt ttcatttata tgctcctttc
     7201 aatgaacaaa tcggaatttt acgatttatt aacgtgaact aatgtatcaa aataaaaaaa
     7261 cacagtcaat agaaatccat caatttctga tttaagcatg tatattcttg attaaatcgt
     7321 aaaatggatg agttgctgct catatctagc catttcacta caataaaaaa ctgaagaata
     7381 ccattttagg attcctcagt ttttcatcac ctattttact tcttatattt tattgcaggg
     7441 ttcgtactaa ataaacttcc tcacaaaaac cttttaaccc attatatact ctctgcgctc
     7501 ttgaataatg ttgacacaag gcaaaaacgg ttggtccgct tccactcatc aatgcagcat
     7561 ctgctccgta cttgatcata cgctctttga tttgatggat gactggatgc cgctttcccg
     7621 ttatatcttc taaactgttt cccatttttt tgatcatctg ctgataatct tgctctcgga
     7681 tggcttcagc aagtccgtgg atgtcttgat gttggatggc ttgcgtatct acttctctaa
     7741 acactgtacg tgtagagaca ctcattcttg gtttgaccaa cacaacccag cactgtggca
     7801 tagaaggtaa tacttggatt tcttcccctt tgccaccgac aaatgccgta cttccgtaga
     7861 cacagtaagg aacatctgta ccgacttcca aaccgatttc tgccagttct tctaacgaac
     7921 agcccagatt ccataattgg ttgatccctc ttagagcagc tgcacagtct gtacttcctc
     7981 cgcctaatcc agctgctact ggaatattct tcttaacaaa gatcttgaca ccggttttga
     8041 tattgtattt attttttagc aataaagcag cttgataaac attgttccgt tcatctaccg
     8101 gtaaaaatgc tttatttgtt tctacaatga ttttgtcttc tgttatttct tcaaaaaata
     8161 gatgatctgc caagtctacg ctcgacatca ccatttccaa ctcatgatac ccatcttgcc
     8221 gtctatataa tacatccagt cctaaattta tcttggctgg cgctttttca atcaattcca
     8281 tcctctcacc cattcgaaaa gtctaagtcg caagtattct atcataggga aaaataaaag
     8341 tatagcttat gtctgcctag tggttgattt tgtcatcgtt tcaacagctt tcacagaatt
     8401 ttctcttcat aatccaaaca agttctagtg attcttataa ttctgtgagc acttcttctg
     8461 ccaaactcgg aacatttcct ttttcttttc ctatcagact gagtagctgc atggtagcaa
     8521 atccgaaatt acttcctttc ctataagcaa aataacgtcc ttcccaatga tcgacatatt
     8581 ttaatttctt ttcataagca gactggaagg aatagaaaaa attaccataa ttatatacga
     8641 tatgcatgac tttttcttga aagaaagaaa aatctgtttc tccaacatta gctaatggag
     8701 ctaagcctag attggcttcc gagtagccca ttttctcgta tacaataaac agattggcta
     8761 acagatagtc agctaattcc tcagattctt taggtaagac ccgcaacaaa tcatatcccg
     8821 cctgcttctt cgtcatcggc tgagcggtga tgaagccaac gactttttga tctttacgga
     8881 caataccgac accgctagat aatacatatg atttatcaaa tcgtcccaca gagaagtatt
     8941 tttctcgtgc accttgaagc catttttgtg aaacttcatt tacagcttga tgtaattctt
     9001 ccggcagttg atcgtaccaa gtaaaatggt atccttcttt catcaaacgt tgatattcaa
     9061 gttgctgact cagccgagac aagtcttctg ttgctagatc aacaatccct gcttcaccta
     9121 ctttcgtgaa gtgaaaaccc aattcatgaa gcatcactac gtagctttct gatatcttat
     9181 aaaacgcaag ctgatagcct aaacaatctg cttgtttcat aaactgattc gttgctgccc
     9241 gcatcatttt aggattgcca actggatctc ctaaaacaaa gcatttgttt cctttgatct
     9301 gataaccaaa aacgacttga tcttggtcgt cttcttggta ataaaaataa ctgtaacctg
     9361 acaagcgaag ctgatggctt gtagctgtgc ctccgtatgt ttccaataaa cggtcaaaac
     9421 ggtctttttc gtactcactg ccaagcttcg tagtatcttc agctaaatat tgataaagcg
     9481 ccactaaagc tagcaatgag atgcccaaac caataagccc tgagaaccac acatcatctg
     9541 aaggaaacaa aataaaatga tttcctgtta ttcgaccgac taaaaagcgt ccgctgtgat
     9601 agcctgcaat tgcgtaaacg ataaacaaaa agccgaacaa gctagcatca aagattatag
     9661 ctccccaaga ataaacgaat tttgttcgat aaaattcttt tctggataac cacacagcta
     9721 acagaatcaa gacatatacc aatatcaact gccaagataa cgtacgtgtg attgtattta
     9781 aaataccaaa agatagtaaa gcaatcgtag gcagaaatgc ctttttgact tttactgaaa
     9841 gtgctctagc aaggccgagt aataaaaagc caaccaatag attcactgtc tgatctaaaa
     9901 aattgaatga gaacggcaat aatacttgaa atagccgact gattgcagac agattagtga
     9961 ttgttgatag aagtaccatc atgatccccg caaaatagac agcacttacg agcacgatat
    10021 gcgcaacctt ttggaaaaac agtcgcggaa tgttatctaa aaatcgattg attttgactc
    10081 ctgtatgtgt gatgaacaat agaagtcccg tcataaaagg tagaacataa taaaacaacc
    10141 gataatataa tagccaaact actgctacat cttgccgaac agacaattga ctcattccta
    10201 agatcatcaa aacgtcaaaa gtccccattc cacctgggac catcgtcagc attcctatca
    10261 atgtagcaat catgaacata ggatagatcg aaagcatcga aatatctatt ctcatcaact
    10321 tgccgatgat caaaaatacc gcaagggctc ccgtccattg tccaaaggat gccagtagca
    10381 gaccaagcaa gccttttgga taaaactcga agaacaatgt ctttctcttt agataagcaa
    10441 aaaagaaaat gactggtgct aaaaaactac cgccgaaaag ccagatccaa tagcttcgaa
    10501 aaatactttc tgactgaata aaaaagacat caataaatgt aagaaacgac catacagaca
    10561 aaccagtcag cataaatagt gcgacttttg atacagtggc taaaaccatt tttctatttg
    10621 tctgctttcc ataaaaacta gcccgcagac ttgctcctac cacaccacca aaaccggcga
    10681 gattgttgat cgtgtttgtt acccacgcag caagaagaaa ctcttttctt tccatttttg
    10741 gttttccttg tttttctaag actctgatcg tcacccaatc atacaacaac atcggagtga
    10801 cacccagcag tccagccaga atcatgagca ggatcgtcat gcggctttgt tgtcccatcc
    10861 tttgccaaat atcttgccaa ctcatgccat tagcaatatt tgcgacttga tttgccacaa
    10921 agactaacac ggagccgaaa aaaatcagtt ttaaaatcag actatgttcc tttatccatt
    10981 gcagcatcaa gtatcgttta tttttcacat tttcccattc ctaacttgta tacttttgtg
    11041 tcatacccca atgttatctt atttatttta atttttccac tttagacctg ttctttgctt
    11101 gcgatgatag aaaaagaaag atatcctaat gctaaaggag aggataacat gaaagtagga
    11161 tttatcggca cagggaatat ggcacaagct attattgatg gtttattaaa aaaaggaaca
    11221 gtctcgaaag aagaggtgtt cgtttccagt ggacattttg aaaatgcaca aaaatttgct
    11281 gctgaaactg gcgtccatgc atgcaaaagt aatcaggaag ttgcatcaca atcagatatc
    11341 gttcttttag ccgtgaaacc aaaaatcatt gaaaaagtat tggaagaact aagagaatat
    11401 agccagaaag tcttattcat ttctattgct tcaggaaaat cattaataga tttagaaaca
    11461 gcgcttgaag atcctcaggc ctccatcgta cgtgtgatgc cgaatgtcaa tgtcagcgta
    11521 ggagcaggcg tttctgcgat ttgcaaaaat gaagctgcca cggaggaaca gcttcagcaa
    11581 gctacgatga tttttgaagc aatcggcagc gtctatcctt tagcagaatc agatttcagt
    11641 acattcattg gccttgctgg cagctcaccc gcattcgttt acatgctgat cgatgcgatg
    11701 ggacggactg gtgtactgaa tggtttgtcc aaacagacag ccactgagat tgctgcaaaa
    11761 gcaattatgg gttcttgtca aaaattcctt tcaagtgata aaaatccatg ggaacttgta
    11821 gaccaagttt cttctcctgg cggtacaact gttgcaggtg tagttgcctt agaagaagca
    11881 ggattcatcc cagcaatcat caaaggaatc aatacgacga tcgagaaaga tcttgagatg
    11941 ttgaataaaa aataatgata gcaatgtttt ttcgtgcctt acaaaaaaag aagagctgtg
    12001 gcttttgccg cggctcttct tttgtatttt ttattattta gcataatcag ttgcacgagt
    12061 ttcccgaata acgattactt tgatgtgtcc aggataatcc agttcttctt cgattcgttt
    12121 acgaatatca cgtactaaac gaacagcgtc taaatccgag atttcttctg gcttgaccat
    12181 tacccgaact tcgcgtccag cttgaactgc gtaacttgat tctacaccgg cgaagctgtt
    12241 cgaaatattt tccagacttc ttaaacgacg gatgtagttt tctaatgatt cacttcgcgc
    12301 accaggtctt gctgctgaga gcgcatctgc tgcagctaca aggacagaga taacagaagt
    12361 agcttccaca tctccatgat gagaagcgat cgcgttgatt actactgggt tttctttgta
    12421 cttggcagct aattccgcac cgatctcaac gtgagatcct tcaatctcat ggtcaagtgc
    12481 tttcccaata tcatggagca acccggcacg ttttgctaat tggatatctt cgccaagctc
    12541 tgctgctaag ataccagcta attttgcaac ctcgaccgaa tgcttcaaga cgttttgtcc
    12601 ataactagtt ctgaatcgca gacgtccgat gatcttgatt agatctggat gaagtgtatg
    12661 agcaccgact tcaaacgctg cctcttcccc gtattcgcga atacgttcat ccatttcttt
    12721 acgagatttc tcgaccattt cttcgatacg cgctggatgg attcgtccat cttgtatcag
    12781 tttttccaag gtcatccgtg cgatttctct tcggattgga tcgaatccgg aaagaacaac
    12841 tgcttccggt gtatcatcga tgattaaatc gataccagtc agcgtctcaa gcgttcgaat
    12901 attacgacct tctcgtccaa tgatacgacc tttcatttca tcattcggaa gggtaacgac
    12961 tgaaacagtc gtttctgaaa cttgatctgc tgcgcagcgt tgaatcgcta atgataataa
    13021 gtttttcgct ttgcgatctg cttcttcttt ggcacgctgt tcagattctt ttaccattag
    13081 agttaactca tgattcagct cttcttcagt cgatttcatg atgacatctc gtgcgtcctc
    13141 ttttgataaa ccagcaattt tttctagttc tttttgttga tcttcaatca ttttttcaac
    13201 gtctttttct cgctcttcaa ttaattgctg ttttgaacta agtgcttctt ctttactttc
    13261 aagtgagttc tcgcgttttt ctaacgagtc atctttacga ttcagcgttt gttcgcgttg
    13321 aatcaaacga ttctcttgag attttaattc taacttactt tctctcagct cactttcaac
    13381 ttcagaacgg tatttctgat tttcttcctt agcttctaac aatgcttctt tttttagagt
    13441 ttctgcttct ttttttgcac tatcgatgat tccagcagct gatgattgtg caccagctat
    13501 ttctttctcg tgacgtgact ttgcgaccat aaaccctaaa ccaagaccga caattaaacc
    13561 gatgatagcg aggagaatgt tgaataccat aaaatccacc tccatactat ctttttcatt
    13621 tttatcatct atacagcttc tatgataaat tattctaaac tcgtcaatat gcatcttcgc
    13681 atatatgtgt acaaaaaaca tgcacaactt tattctaatg tttgtacccc gtagtgtcaa
    13741 ctttaaataa acaaagaaac ggctttctac ttattcatta aacgatggtt tttaaaataa
    13801 atgacactag tttcatatag gtaaaccttt aaattgagaa acttttctat taaattattc
    13861 tttgtagatg aacagatatt tttattttat gtacccactc gtataattaa ataactcacg
    13921 atttctttat tcaataaaaa aacggcaggt caaggagagt acctactctt cagaccagcc
    13981 gtttttgttt atcataattt ttattcttct actaaaggca attcttcttg tcctttttca
    14041 tcttctggga cagcgacttc ttcaccaata ccataagctg ctctaactag agcagaaact
    14101 tctgccatca tttcaggatg attagccata tagattttag cgttttcccg tccttgacca
    14161 atccgatctt ctttataaga ataccaagca ccgcttttat caacaatgtc tttttctact
    14221 gccatgtcta gaagttctcc ttcttgtgag atccctaaac catacatgac atctacttca
    14281 gcgattttga aaggaggtgc tactttgttt tttacgactt tgatctttgt acggttacca
    14341 acaatatctg taccttgttt caattgttct gcacgacgta cttccaaacg gatcgttgcg
    14401 tagaatttca atgcgcgtcc tccaggagtg atttctggat tcccaaacat cacgcctact
    14461 ttttcacgga tttggttgat gaaaatagcg attgtttttg ttttgttgat cgaaccagag
    14521 agtttacgca atgcttgaga catcaaacgt gcttgtaacc cgacatgtga gtcacccatt
    14581 tcgccgtcga tttctgctcg tggaactaaa gcagcaactg aatcgactac tacgatatct
    14641 acagccccac ttgatactaa agcatcagcg atctctagac cttgttctcc tgtgtcaggc
    14701 tgtgaaagaa gtagttcatc gatattcaca cctaattttt gtgcatattg cggatctaac
    14761 gcatgctcag catcaatgaa agcggccgtt ccgccatttt tttgtacttc tgcaatagcg
    14821 tgtagtgcaa ctgttgtttt acctgaactt tcaggtccat atacttcgat gatacgtcca
    14881 cgggggtaac cgccgacacc taaagcgaca tctaacgcaa gagagccact tggaattgta
    14941 gagatttgtt ggtcgatttt ttcgcccaat ttcataatag agcctttacc atagctcttt
    15001 tcaatttttt tcagtgcagc atctaaggct gctttacgat catctgccaa tacgataatc
    15061 ctccttatat ttacaaatca gagcaacatt tcattatcct tatcatatct tttttagtga
    15121 aaaaaagcaa gaaaaaaccg aacaaatatt cgcatttatc attttcttaa aagctgtttt
    15181 ctcaaaagat caaatccttg cataaccgca ttatatctga tagcagcacg atctctggca
    15241 aagtgatatt tttccgaaac cacgccgtct ttagatgaaa tagcgatcca tactgttcct
    15301 gcaaattgac cttctaacgt gtccggtcca gccactccgg tgaaagctac actgtaatct
    15361 gttcttgcta tctcacgtgc ttgaatggcc atctgctcca cacattcttt gctgactgtt
    15421 ccatattctg ctaatagctc cggatcaatc tccagaaaac cagcttttgt ttggagcgag
    15481 taggtaacga atccgcctgg aaatacttca gatacaccag caatgtcgcc taatgcggct
    15541 tggaaggcgc cggctgtcaa actttccgct gctgttacag tctgcttgtt ttctttcagt
    15601 agttccacta cgacttttgc tagcgaatta tcatcgccgt atccataaaa atattcccct
    15661 actcgttctt ggattttttc ttctaatgct aaaagagctt gattccctgc ctgaacatca
    15721 tttgttttca ctgtcagtcg taatgtcact tcatttggtt tggcatatgg tgcgatcgtt
    15781 ggattgatct gtgtctcaat cagatctttt agctcagtga ccagtcgcga ttcaccaatc
    15841 ccataaaagc gcaaaacttt agaaattagt ttttcttcag aaggaaaacg ttcctctagt
    15901 aaaggacgta cttgttccac aaacattggt ttcaactcat taggcggacc gggaagaagg
    15961 atatacgcat gtgtatctgt ttgataaaaa gtccctaaag caagccctgt acgatttggt
    16021 agaggaaccc cgccttcgat aatctgggac tgttgcagat tgtttttggt catttttcga
    16081 tgggttgttt caaaatatgc caataatttt ttgtatcctt ccgtattttg tatgagagac
    16141 ttaccgaggt gagccgcagt tgcttctttt gtcaggtcat cttctgtcgg tcctaatccg
    16201 ccacataaga gtatgagttc acttcttgtt tcagctagag caatcaactc ttctaaacgc
    16261 tgctgattat ctcctacaac tgtttgataa taaacttcga tacctagatc agctagctgt
    16321 tctgacaaga aagtggcatt cgtattcacg acttgtccta gtaaaagttc tgttccgaca
    16381 gctattattt ctgctttcat tacttcccgc tcccttatat agtatacgct gtatttttat
    16441 atttttttat ttaaaaagag ggcaaggagc tgtgacaaaa atcttgtcac agctccttgt
    16501 tccaaataaa cagtgttctc ctgccaaaac ctcggtcata agtcacaata tacataaaac
    16561 atgaaaagct gttttttgaa attatccctt gtgactcaga gttagacggc aggctcacaa
    16621 cctctccact aaacgatact tacattgatc ctttgaacac accgctgttt ttgatgaagt
    16681 aatccacacc tgaatagata gtaaagatca gacaagcata aagcatgatt tgatctactg
    16741 gaataccgat caggctgaat gggatattat tgataaacag caagataata gcaaccattt
    16801 gtgtagccgt cttgactttt cccggccaag ctgcagccat gacttctcca tgttcgacaa
    16861 gtagtaaacg caagcctgtg acggctaatt cacgacagac aatgatcgct actacccaac
    16921 caggggcctt tccttggtca accaacatga taaaagcagt cataactagc attttatctg
    16981 ccaacgggtc cgcaaacttg ccaaaattag tgacaaggcc tcgcgcacgg gcaatttttc
    17041 catctaacca gtctgtgata ctagctaaag caaaaataac tgcacctact agctgtgtga
    17101 cagccagtgt ggtgtctcct acgttaaatt gtccccaatc taaaggtaaa cctacaacca
    17161 aaatgaaaat cgggatcata caaattctaa gtacagttaa tttatttggt aaattcacac
    17221 tatcgactcc tttcttccct attctacgtg actatcagcg aagtttccac cgatattatt
    17281 tacttttcca aatgaattta ttcaaaggag tatttttatt gaaattgatc tttttgaatt
    17341 aacgaagaag gaccgcgaca cagatttgtc acgatccttc ttttttatac ctacaagtaa
    17401 acagatgcgt aaactaattt atacgatcat tctgcgtatt gaagagtaag gttcacattt
    17461 tttggactct gattgttttc ccctggatta actggtacgg attctccatt cgctttgatc
    17521 gttgcattgc ttgctgcacc aatcgtgatg gtcgcatttg ttgctgtttc cggtaacacg
    17581 gtactctgtg tttcatttgc tgccagtgtc ccttgataaa cataggcatt gtctactagc
    17641 acacctaacc atacacgatt gacagcagta aattcaaggg ttacaggctt ggtagcgtct
    17701 gtgatattta ctgcgatggc actgccagaa tcttctcctg tagctattgc cattttcttt
    17761 tcttcacttg attttgttgt tgattctact gtagaagact ctttggtctg cgtgctttgc
    17821 tctgaggaag aggactcctc gactgatcct tctactttaa gcgaagaagc tgtgtctccg
    17881 attattggat cagcttgacg atcttgccaa gtcatatagc ccacgacaac gacaatcgcc
    17941 aaagcaacca atcctaataa aatgacagga agacttgtcc aaaagcgact tggatgcgat
    18001 tcttcctgat gcattgcttt tctagaagta cttactgttt caggctgcgg acgttcaggg
    18061 accgatcctg tcaatttgct tttcccatca aaaacatcga ctaaatggtc tccatcttct
    18121 cctacagctt cagcgtattg ccgaataaat gcccgcacgt aaaatttacc aggcaattgg
    18181 tcaaaatcat cattgtcgat tgcttccaaa taacgttttt gaatttttgt aatctgctgt
    18241 aattcatcta aagatatttt tttattcagg cgtgcttgcc gtaatttttc tccgatggtt
    18301 tcaaaagcca ctcgttcatc ttctccagtc tgtttgattc tccgaagcat ctgctgttgc
    18361 aacgaaagac gttatgtttg ggtgctgtga caaaagtcat gtcacagctc cttttttccg
    18421 aataaacatt gttctcctgc caactcctcg gtcacaagtc acaatatcca ataaatataa
    18481 gaagctgttt tttgaacttg ttccttgtga ctcggagtta aacggcaggc tcacgacctc
    18541 tttcttaaac ggtgttcaat cagctgttcc tcggtattag ctcaaaaacc ggcaaaatat
    18601 aaggagatat ttttccgtat gtttctctaa tactcaggtc aaaacgctga tttcacaacc
    18661 tcttccttaa acggtgttca aaagcttgca tctgcggtaa taagtccaac caagttaaaa
    18721 acattaagaa gctgttttta cttggctgtt cttattactt gatgcagggc agctttttca
    18781 caacctcttg gtacacggtg ttccagaagt agcttcttcg gcaataagcc gaaatatcac
    18841 aaaaatttga gaagcaattt tcgtaaattc cttcttattt cttgaagctg accacttctg
    18901 tcacaacctc tttgattact tcgtttgatt ttattttaat ataagttgat taaaagagag
    18961 catgatttgt tttattttct cactaatttt tccaacacaa ctaagaatac cataaaagct
    19021 gattttggag gaggttccta ctacaagtct aaaacgaaat ttaagacttt tcctcataaa
    19081 atactggttt attttggtct catgtaaaac tcgctgaagg cctcttcact tataaatgct
    19141 tccccagctg ccagtacgtc tttcatttgg atcgaatcga tgatttcagg aagatcaaat
    19201 aaagttcgat cgccaaataa actttgcgta aattgattgg caatatattc gatcgaattt
    19261 aatgattgga aatattgtcc aagcattttc ttcttcagca aatcgagatt tgtttctgac
    19321 acttccggat catcagcgaa tccaagaatg atttctttga ctttttctgc tgctttctct
    19381 ggttcgtccg tatctccgct gaagtccgca aagtgaaatt cacgatccaa gctgaattca
    19441 aaaccaaagc tgtcatcgat aattccttga ttgtacatcg ctagatagtt tcgagaagta
    19501 ttgcctaaaa tcatttggaa caacagattg attgctgttt tatagcgtaa cagttctgtt
    19561 ccttcttgcg gcaatgtgtc taatccttta ataccaagta caaatttatc tcgtgtaatg
    19621 gctgcttcca atgcactttg tttgatgatt tcttttgtgt tttcagggaa ataacgtaca
    19681 atttcttgct ttggagggaa attttttgct tcttggtttt cccgaataag tttcatcaat
    19741 ttctcaggct ccatcttacc aactacaaat aaaaccatat tgcttggctg ataaaacgtc
    19801 cgatagcacg tatacaagtc ttgagctgtg attttgtcga tactttcaac cgtgcccgca
    19861 atatcgatat gcaatgggtg tgtaggatac aagttattta gtatgccaaa aaacatccgc
    19921 cagttagggt cgtcttcata catttggatt tcctgaccga tgattccttt ttccttgttt
    19981 acagtttctt ctgtgaagta aggtgcttgt acaaaatcga tcagagtcgt cagattcttt
    20041 tctacctgat ccgtcgtaga aaacagatag ctggttttcg taaaacttgt gaaagcattg
    20101 gcggaagctc cttgtttccc aaataattga aagacatcac catcttcttt ttcaaacaac
    20161 ttatgttcta aaaaatgggc aattccatct ggaactttca ccttttcttt ttcaccatag
    20221 ggaataaatt cgttatcgat cgaaccataa ttcgtactga acaaaccata tgtcttgtga
    20281 tagtcatttt taggcaacaa atacacggtc aaaccgtttg gcagtacttc atgataaagt
    20341 gtttcattga tttgctcata ttctgtttta ttcatttgca tttcctcctg ctaaaaagaa
    20401 gattgcctgc aattcgattt gctcagctac ttgttgtatc tctttgatcg ttacgctctc
    20461 catacgtttc aaccattctt catcagataa ttttgtttcc ggcagccagc tgtccaaaaa
    20521 agaagcttcg attgccgctt gcggagagtc tagagaaagc agatattggt ttctcagcat
    20581 tgcttttgtc tgagacagtt ccaactctgt tatttcacca tttcgcaaac tttctaattg
    20641 ctcgtgaatc agatgaagta cttgatttcg gttcttttca tcgataccgg tttggacact
    20701 catgaatcct cggaacgtat ccacgctact agaggcataa taagctagac tttctttctc
    20761 tctgacattc atgaaaagtt tagagtgagg aaaaccgcca aataaaccgt taaagaccat
    20821 tagcgcaaaa cgttctggtt catcgtaata cacatttgtc tgataagcta ggttcagctt
    20881 tgcttgaacg attggctcac gtacttgtcg ttccttcacg atattcactt gaggttgggt
    20941 ataaaacaga tctggataaa ctgtttcccg cgactcaaat ggtaattgcc caactgcttc
    21001 agctgctttg tccggatcaa catcgccgac aacaaaaatg tcgatttgat catttgccat
    21061 catttcttga tagtaggacg ctagcgaact agcagtgatc ttgtccaaat cttcgactac
    21121 gcccaaccct gggatctttt gttccgggct gttttcaaaa taaagttctt gcaaggcata
    21181 ggaagcaaaa gtttgtttat cttccttcaa actttccaaa tatagacgca gattgttctt
    21241 ttccaagtca aacgtcaact gatcaaactg atgattttta atatttggat aaaataaaac
    21301 ctcttttaaa aaatctaccg cttgagaaaa aaggtttgga tcattgacat attcaccatt
    21361 cacaaaactg atccctgcgt ttagccaatg taaatttcct ttttttctaa ctgataatcc
    21421 aaagcttgcg ccataaagct ctgcaagttg cgcacttaac tttgtctgat ccgaataatt
    21481 caaactattt gtttccaaca tgctgctgag aagtgaacgt ttcgtgatga cttcttgttg
    21541 cagacgcgca gtaaaacgga taaaaatccg gatcgttttg tattgttttg ttggtaagac
    21601 atgaagattc acgccttctg ccaattgata caccatattt atatgctcct tttcacagtt
    21661 gattgcccgc ttactctttt aatgggtcaa cctgcctttt ttacttattc ctctgcttga
    21721 aatttataag ttcaggcaat tcgccgtttt ttctaaatga tgttacactt tatagtatag
    21781 catagatgtt ttttatcaaa agaaacagac ggctaaaagc gtatattgtt tttttatttt
    21841 ttatttttcg atatactaaa gacaaataaa agaagggagg gtcattcatg atcaaagaat
    21901 tcaaagagtt catcatgcgt ggagatgtct tggatcttgc tgtcggcgtt gttattggta
    21961 gtgcgtttac aagcatcgta aaccaagtgg tgcaaggctt gatcacacca ctaataggtt
    22021 ggtttgtcgc aatgatcaca ggtacttctg atttagaagg agcactttca attcttgact
    22081 ggtcgccgac aaaaggcgtc acatttgcgt ttggtaacgt tattagtgct atcattacat
    22141 ttctgatcac tggttttgtc ttattcttag ttgtcaaagc ggcaaaccgt gcgaagaatt
    22201 tacgctccaa agaagagcaa gtggaagtgg aagaaactcc tactgcagaa gattatctta
    22261 gtgacatacg cgatcttctt gccaaacaag tggcagaaga caatacacat ccagtagagc
    22321 aaacaaatct ttccgatact tctaaataat aaagaatcta aaaaaaagaa agccgatttt
    22381 cagtgtcggc tttctttttt tagattctta acttgctact tgttcctttt tcatgtcgat
    22441 atgtggaata tcatcttcca aatacacatc tgagattgcc ttgaatccaa acgactcata
    22501 aaaagcttgt aggtattctt gtgcgccgat cacgattggt ttttctggaa aatttttctg
    22561 gatcatgtta atcgtttctt gaacaatctt tcttcctaaa ccatctttac ggttgtcctg
    22621 atgaacaagg acacgtccaa aatggatact ctccgcttct tcatatactc gcgtgtaggc
    22681 aaggatcttc ccatcagtat cttgaaaaaa cgtatgccaa gcttctccat caatgtcatc
    22741 aacttcttga taaggacact gttgttccac aacaaaaact gcgattcgtt ctttgacgat
    22801 cgcgtaaaac tctgctgttg acaattcttc aaatttcttt acctgatatt ccactaagca
    22861 aacgccccct gcttttttat tcttagcata ccgattcgca cacaaaaaag caaacgaacc
    22921 ttacgaatct caggctcgtt tgctttttaa actgcttcag ttaaaaattc gatctcaact
    22981 gtccgagtaa gtacatctga atagctgtaa gatactcgtt caaaagaatt ttcttcagga
    23041 tccaaatcaa caacgaatac tgaaggatag gtttctgtta aaacgccgcg gcgttctgtt
    23101 tgacgctttc ttcctgtctg agcaaccaac atgattgggc tgccgatacg accttctaaa
    23161 tctttcttga ttgaagctaa agttgttggc atacagttca cctctactat caactatacc
    23221 acatattttt aaaattttca agtttaccat aaaaaaaaaa gaaatgcaat aaaaataaat
    23281 aagaagctca ttaagatttt tctaaattat tcctcattat aaaatttcaa gtgttttttt
    23341 attttgagtt ttactctccc gtaagcgctc attacttgct tgtgatccat tgctaaacat
    23401 tcagccatgt ctacaaaatc aacgccttgc atataataat gaaaaatttt agcttctatt
    23461 tttgaaaact ggatttcgca attttcgatt gcttctttta ataaaagatg ctgaattgct
    23521 aaaggttctt gcaatgtgat ctctgctaaa aagtctggtc cttctgtctc tattttttgg
    23581 tcgattgaga cttcatcttt ttgatcacga cgcttcagcg cgttctgctt cctcaacaga
    23641 gaatagattt gattgcttat aacgctttta taataacttc caaagctaac acctttgctt
    23701 ttttcgtaag ttctcaatgc ctgatataaa gcgatccttg cttcttgttc ccaatcctca
    23761 gaaacataat ttcttaaata ataggtgttt ttcaaacgta gtaaaagtgg aaaatatttt
    23821 tgatacaggg cagcaaatgc ttcttcgtca ccattacgtg cagcgcgcaa taactcctca
    23881 gacataaaat aattcctcca taaattcgtt tatggaggaa tcttatcatc aatgattcat
    23941 cgaactgatt agaacgatat gttat
//
LOCUS       DACDUU010000041        23790 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.041, whole genome shotgun sequence.
ACCESSION   DACDUU010000041 DACDUU010000000
VERSION     DACDUU010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.041"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(461..1090)
                     /gene="udk"
                     /locus_tag="IWL30_001650"
     CDS             complement(461..1090)
                     /gene="udk"
                     /locus_tag="IWL30_001650"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAP9706407.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1174..1869)
                     /locus_tag="IWL30_001651"
     CDS             complement(1174..1869)
                     /locus_tag="IWL30_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAP9706408.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1882..2598)
                     /locus_tag="IWL30_001652"
     CDS             complement(1882..2598)
                     /locus_tag="IWL30_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAP9706409.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2928..3536
                     /locus_tag="IWL30_001653"
     CDS             2928..3536
                     /locus_tag="IWL30_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAP9706410.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3861..4184
                     /locus_tag="IWL30_001654"
     CDS             3861..4184
                     /locus_tag="IWL30_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP9706411.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4366..5736
                     /locus_tag="IWL30_001655"
     CDS             4366..5736
                     /locus_tag="IWL30_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAP9706412.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVNPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5866..7167)
                     /locus_tag="IWL30_001656"
     CDS             complement(5866..7167)
                     /locus_tag="IWL30_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP9706413.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7521..9512)
                     /locus_tag="IWL30_001657"
     CDS             complement(7521..9512)
                     /locus_tag="IWL30_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAP9706414.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9656..9922)
                     /locus_tag="IWL30_001658"
     CDS             complement(9656..9922)
                     /locus_tag="IWL30_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9706415.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10432..12558)
                     /gene="pnp"
                     /locus_tag="IWL30_001659"
     CDS             complement(10432..12558)
                     /gene="pnp"
                     /locus_tag="IWL30_001659"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAP9706416.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12746..13015)
                     /gene="rpsO"
                     /locus_tag="IWL30_001660"
     CDS             complement(12746..13015)
                     /gene="rpsO"
                     /locus_tag="IWL30_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAP9706417.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13266..13829
                     /locus_tag="IWL30_001661"
     CDS             13266..13829
                     /locus_tag="IWL30_001661"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP9706418.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14082..14603)
                     /locus_tag="IWL30_001662"
     CDS             complement(14082..14603)
                     /locus_tag="IWL30_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAP9706419.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14606..15448)
                     /locus_tag="IWL30_001663"
     CDS             complement(14606..15448)
                     /locus_tag="IWL30_001663"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAP9706420.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15441..16166)
                     /locus_tag="IWL30_001664"
     CDS             complement(15441..16166)
                     /locus_tag="IWL30_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAP9706421.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16342..16638
                     /locus_tag="IWL30_001665"
     CDS             16342..16638
                     /locus_tag="IWL30_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706422.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17206..18798
                     /locus_tag="IWL30_001666"
     CDS             17206..18798
                     /locus_tag="IWL30_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9706423.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18811..19551
                     /locus_tag="IWL30_001667"
     CDS             18811..19551
                     /locus_tag="IWL30_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9706424.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19719..21068
                     /locus_tag="IWL30_001668"
     CDS             19719..21068
                     /locus_tag="IWL30_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9706425.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21196..21786)
                     /locus_tag="IWL30_001669"
     CDS             complement(21196..21786)
                     /locus_tag="IWL30_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP9706426.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21933..23285
                     /gene="brnQ"
                     /locus_tag="IWL30_001670"
     CDS             21933..23285
                     /gene="brnQ"
                     /locus_tag="IWL30_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP9706427.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6819 a         4800 c         4514 g         7657 t
ORIGIN      
        1 tagggggtgc gacaaaagag cttcttcaaa aaaataagtc gaaatctcac acaaattcga
       61 gaaataattt tcgtaagttt cttcttattt attgcatctg accacttctg ttacaacctc
      121 tcgttaaacg gtgttcaaaa gcttgcatct acgataataa gcccaaccca acgaaaaacg
      181 tgaggagctg ttttatttgg atgctcttat cgcttgatgc aaagacagct tttttacaac
      241 ctctacttca cggtgctcaa tcagctgacc ttcggtatta gctcaaaaaa cggtaaaata
      301 taaacagcta ttttccgttt cttcttttta atacttaggt caagatgctg ttttcacaac
      361 ctcttgttat aaataaaaaa acagttggct ggtgactgtt caaaaagcag aatccttgct
      421 tttcaaacgg tcgcagtccg ttcaactgtt tcgctgcgca ctatttatgg tttaagaaac
      481 tagctacttt tgttgtaatc aaatcaatag caacgtggtt ttctccgcct tctggaacaa
      541 tgatatccgc atagcgcttc gttggttcga taaattgatg atacatcggt ttaacaactg
      601 tcaaatattg ttcgatcacg gagtccaatg tgcgtccccg ttcttccata tcccgtttga
      661 tacgacgaat gatacgaatg tcatcatctg tatctacgta tactttgata tccatcaagt
      721 cacgcagacg ttcgtcttct aaaatcagga ttccttctag aatgatcact tcttttggtt
      781 cctgaatgat cgttgcctga cttcttgtat gcgctacata gtcatacacg ggtttttcaa
      841 tggctttata gttcagtaag tcgcctacat gctgaatcag cagatcatta tcaaaggcaa
      901 atgggtgatc gtaatttgtg ttcaatcgtt cttcaaaact caaatggctc tgatccttgt
      961 aataagaatc ttgttcaagc atcatgatgg aatggtccgg gaagttgttg aaaatcgcac
     1021 ggctgacact tgtctttccg cttccggatc cacccgtcac accaatgata attggcttgc
     1081 tttttgtcat tgactaaaac ctctttcgta tccgtttttt cattctaatc tattatagcg
     1141 aaaaaacacg aaaatgctat atttttctga aaattaatcc ttttcaattt ccaataatga
     1201 ataaagttcc tctaatgaat cgattcgata agtaggctgg atttcttcga tttctggtaa
     1261 tttatttcta ttgaaccaaa tcgtatcgat ccctgccata tttcctcctt tgatatcaga
     1321 agtcaaagaa tcaccaatga tcacagtttg ctttttatca aaattaggaa tgtgggaaaa
     1381 gacatgatca aaaaattctt tcatcggctt ttgcgctccg acagcttctg agacaaagat
     1441 atccttgaag taatcaagca ttcctgcatc tgttaatcgc cgatgttgcg tgtgcgacac
     1501 accgttcgtc acaatataaa gatcagcatg cggatggacc tttttcagga tttctagact
     1561 tcctggaaca agatcatgac cttggttcaa gtagtggcgg tagcgttttt ccatttgatc
     1621 actatctact gttttcccaa attgctggaa aagtaaccca aaacgactgc ctattatttc
     1681 ttctcgtgat agtctgcctt tttcaaactc ctgccataaa tgatgattca aagccttata
     1741 gctggtttcg atttctggtg tcaatgtgat tccttgttct gcaaacaaag cacttaaagc
     1801 attggcttca gttaattgaa aatctaacaa tgtatcatct acatcaaaca ataaagtttt
     1861 atatttcatc tttttgacct cctatttaaa ataaatactt aactttttca tctctgctaa
     1921 tgtcaacgat cgatactcgc ctactcctag ctgttcatct aattccaatg gtcccatgct
     1981 tagccgtttc aatgcggtca ctttctttcc ttgggctaag aacatctttt tgacttgatg
     2041 gaattttcct tcttggatcg tcaataaaac cgtactttgg tgcttgtctg ctttgagtat
     2101 ttttaaatca gccggcttac attgataccc atcagcgaat acgattccat tttcaaatgc
     2161 tgttacgtct gccggtgtca ccctttcgtt cacaactact tgataagtct ttgaaacctt
     2221 tttttctgga agtagcattt catatcccaa ttgtccattc gaagtgatca gcaatagtcc
     2281 tgttgtatct ctatctagtc ttcccactgg atataatggt tggaaacgat cggcacttgc
     2341 aatcagatcg attactgtcg tcttttctgg atctgataca gctgtaacag ctttttcagg
     2401 tttattgaga agataatagg actcatttgt ttctaagtgt tcacctgcta cttcgatttg
     2461 atgcagtaaa ctatctacat ttcgattttg tcgaaattcg actgtgccat ctatcatcac
     2521 ttgcttcaaa gcaaacagcc gcttcatttc cttgcgtgtc gtttctaatt tttcttctat
     2581 taatttatct aaacgcatgc tgtttccttt cttattacta ctaattaatt ataccttgaa
     2641 tattcttgct tgcaaataca tcatcatagt tattccttga cttaattaac agttcacgtt
     2701 acggttaaat agtaaagtga attggatttc tcatacacag cgtaatgacc tggtggagaa
     2761 aagttgaatc ctgactttct aaggggagat gcgcgcatct ctccttttgc gcttttttaa
     2821 gcgccctgcc ttcgatgatg tgcacgagat tagcctttag aattcactta aataaaatct
     2881 gtccgttacc aaagcagaca gtcacatttt aaggaggaat tttggttatg tcagtatcat
     2941 tagaagtaag taaaagagaa gtacgtcccc gttcattaag aaacaaactt cgtcacgaag
     3001 ggaaagttcc agctgttgtt tatgggtata aagtagaaag tacccctatc tcattcgatg
     3061 gtttgacact aagcaaattg ttgcgtgaac atggagcgaa cacagtcatc acgatggaca
     3121 tcgatggaca aaaagtcaat acattggttc acaagatcca aacgaacact tttaccaatc
     3181 atttcgaaca tgcagaattt ctatctgtaa acatgtcgga agaaacagaa gtcgaaacag
     3241 aagtcgttct tgttggcgaa tcagtgggtg ctaaatctgg tggagtgtta gctcaaaact
     3301 tgtatacagt agtcgtatct gctacaccag acaaattgcc tgaacgaatt gaagttgacg
     3361 tgacaaactt ggaattaggt gattctatta cagtagccga tcttccaaaa aacgacgatt
     3421 acaaattcgt gaccgacagt gaagagcaaa tcgctgctgt agtcgctcaa agtgaagaag
     3481 aagaaagcac aggtgaaggt ggcgaaccag aagtgatcgg ttcaacagaa gaataatact
     3541 gaaacaatca ggtggtgtta gaagcggcgg gtcagcagct tttaacacgc cttttttatt
     3601 tctaatcgtt tattttcata tggggcttcc acttttgtaa atggctttac aaacgaaaaa
     3661 tcctaattta ccaagctttt cctcgcatac ttttatcgta cctttataaa aatttatact
     3721 ttgaaaaaaa gtattcaatt attgttgcat ttttcctgat tctttattag aatgaaccct
     3781 cgttgaagta agtatttcta gtttatccta ggattaatta taaaaataaa acattcgttg
     3841 attcgaagga ggttaaacag atgtttgaaa gttttgatag cagccgcaac cgttacgctt
     3901 cagttggtgt cgtatcaagt ttgccagatg aattgattga cagtatatgg tttattattg
     3961 acctagatct taagggagtc attccattag aaaatatatt aacctttgat ttgatcgaaa
     4021 atcgtggaaa agtgacgatg catttttctc aagcaggaag caatgtggaa atggatattg
     4081 atttgccgtt tgcctattcg aaccaatttc ctgctcaagt atacgcctat gatgacggta
     4141 cgagagaaac gatcctttta ccaagtgaga ttagacaata ttaaggtgat agataaacgt
     4201 ggatgtccct tattttacag accaggccaa gttgttgatt ctttgactca attcttggct
     4261 tctttttatg gagtttcata aattatcatt gatttctaat tggtattctc aggataaacg
     4321 ctatatacta ctaactaaat tttaaagata taaggagttt aaaagatgcg cgatttaaca
     4381 aaaggaagcc ccgctaaact gattttgatg ttcactgttc ctttactgat cggaaatgtt
     4441 tttcagcagt tctacaatat gatcgatatg atcatcgtgg gtcagacgct aggtaaaaat
     4501 gcgctggcag cggttggtgc aactggaagc ctgacatttt tgatcattgg ctttgcccaa
     4561 ggtttaacag ctggtttagc cattattacc gcacaacgtt acggtgcaaa agattatcgg
     4621 ggtctgaaga aaagttttgc tgctagtgtt gtcattagtc ttgtcgtcac tgtgatttta
     4681 actgtcctaa gtctcctgtt tattcgtccg atgcttcaat tgatgcaaac tcctcctgaa
     4741 atacttgatc aagcacaaac atttatttca attattttat tagggatctt tgcatcagtc
     4801 agtttcaatt tgctttctaa tgtcattcgc gcacttggtg acagccgcac accactattc
     4861 tttctgatca ttgcggtcat catcaacgtc gtacttgatt tgatctttat tatctatttt
     4921 ggcatgggtg tggaaggtgc agctattgct acagtaatcg cacaagtttc ttccagcgta
     4981 ctgtgtctag tgtatatcaa gaaaaaaatg cctttgttac agttacgcaa aaaagatttc
     5041 aaatttgaaa aagaagagtt tgctgtacac ctaaatgctg ctttgccaat ggcgtttcaa
     5101 tcttcgatca tcgctgttgg tgctatcgtg ttacaagcag cactaaatag tctagggaca
     5161 gatgtcgtag ctgctcaagc cgctgctggt cgtatcgacc aatttgcaaa tcagccgatg
     5221 atgtcatttg gtattgcaat ggcaactttc actgcacaaa actatggtgc gaaagaatat
     5281 ggccgaatct tgcaaggggt acgtcaaact ttactgatga gtgtcggatt tagtctctta
     5341 gcaggtgcta tcgtcatttt cttcggtcat ttctttgttc aattgtttgt taacccatca
     5401 gaaacacgtg tatttgaatt agcacaaacc tacttcaata tcaatgggtc tctttactgg
     5461 atcttagcag tcctgttcat tttacgctac acacttcaag ggcttggaca aagcaagatc
     5521 cctacgatcg ccggtatgat ggaattgctt atgcgttctt ttgcagctat cgttcttaca
     5581 ggaatgtggg gatacccagg agcagcagca gcttctcctt tagcttggat cggttctgtt
     5641 gccgtgttgc tttactctta tatgcgttcg atgaaacaat tgaaacacat gggtgaagag
     5701 gaacatttca cattagaaca tgcagaagca agatagcctt gattaaaaga aatcgttgcg
     5761 gctaaaaatg aaaactttta ttgaaaatat cttcttcgtt taccgaaaac aggtttttta
     5821 gataacaaaa aggctgtgac aaaaaatgtc acagcctttt ttcttttatt ctgtttttct
     5881 ttccgtggac tctactttga aataatcgat atgtgcatgc tggtattcta ataccgtgaa
     5941 acggaatgat gcaatctcaa tgattgttcc tgcttttatt tctttatatc ggctagaagc
     6001 aaagcccgca agtgtagtaa aattcgtttc ttcaaactct ggaatctcta catgaaaata
     6061 acgctggaaa tcatctaatg ttagtttgcc ggagatgata taagagccat ctgactgttc
     6121 cgctatcaat gcttcgtcca aatctgtctc atctcggatt tccccgacga tttcttctaa
     6181 aatatcttca atcgtcacta agccgcttgt ccctccgtat tcatctttta agatagcaat
     6241 atgcttaccc ttttttcgca tgatcgtcaa taattttttt atagggatcg tttcagttgt
     6301 tacgatcggc tcttcaatca actgttctac tgtcagctgg tcatctttat aagattgctt
     6361 gatcaaatct ggcaaggtga cataacctaa aacattgtct tttgattcta agataaccgg
     6421 atagcgagta tgtccttgtt caatcgaagc ttttatcgct tctttgacgg tttctgaagc
     6481 ttcgaatact tgcatggacg tacggtctac ttgtatatct tttgccaagg tatcatcaaa
     6541 ttcaaacacg ttttccaaat aataatattc ttctttggtc aactctcctt tttcataaga
     6601 attcaatgcg actaatgtca gctcttcttg agacaatgtc tcttctcctt cagatacgag
     6661 acgcacacct aatagcttcc ctagccctgc agctgaatga ttcaataccc aaataaaagg
     6721 aaacattact ttataaaaat aatgaagtgg tttaactacg aacaagacga ccttctccgt
     6781 attgacgata ctatagctct taggaagcag ttcgccaata accacatcga tatacgtaac
     6841 taaaataaat cccagcgcca cagagattgt actgctaata gccgaactaa aaggtagttt
     6901 accgatcact ggttctagta aagccgcgac agatccttca ccgacccacc cgatgatcaa
     6961 accggttagc gtaattccta attgactagc tgataaatag ttatctaaat ggtgtgtaac
     7021 atgaagagct agcttggcgt tctttacatt ttccttttct agttgttcca atcttgatcg
     7081 tctgattttc actaaagcaa attcacttgc tacaaaaaat gaagtaattg caatcatcaa
     7141 aaataaaata atgaaattga taaccatatt ctcccgcctt tctctttttc tattgtaccc
     7201 tttttctaca aaaagattga aaataatgcg tattcatatt taggaataaa aaaggagatg
     7261 cggcattttt cagccacatc tccttatcac ttcaaaatca ttgcgttact tgttgctaaa
     7321 cagctttttc atgacctctg ataaaaacgg tattcaaaag cttgcatcta cggcattaag
     7381 cccaaacaga tgaaaaacat gagaagctgt ttttatctgc tcgttcttac tgcttgttgc
     7441 agtacagctt tttcatgacc tctgataaac ggtgttcaag tagctgacat tcgacattag
     7501 ttcaggtcaa agcgctactt tcacaaccgc tctttcaacc atgttttccg agtatttcca
     7561 atgataagcg gaacagtttc atgttttact tcgctgtact ctagcccaaa ccatgtttct
     7621 ggcgaggtag cgattttttt gattgccagt tttgcctgca tgatttgacg ttgccatttt
     7681 cctagagttg tcacattgga aactacttca tcaatcagga caaattggaa atcgccaact
     7741 tctcgccctg gtgtcagaga gtatctttgt ggctgttttg gcaaacggcc ttgtttcatc
     7801 aaatcatgga tgacttggcg aacatatacg ttgatttcct gttggaccct gaaacctaag
     7861 aacagattca cttggacaat aaaatcagtg tccatcatat ctacttgata ttctttcgta
     7921 tatggctcat ccgtcacttc cacatgaaca aaccaataga ctcgtgctct tttaggccgt
     7981 ttatctaaaa tcgaatagat gaactgtcgg ccaactttat cttcagccat atcaggaacc
     8041 aagaagacca catttgtctg atagattggt aaagaagtat catcacgtaa ttctctaagc
     8101 tgcgggatat aatctctcaa agacacatcc tcggttgacg attcgcgaat aatgttccct
     8161 tgttcccaaa taaacatcac gactaagatg atcaaaccaa tcagaacggc tacaaaaccg
     8221 cctcggaaga atttgacaac acttgagata aagaaaacga cttctatcgc accgaagaaa
     8281 aaggtaacta aatatgcaag ccaacgagaa tgcccatttt ggataagata aaagtaaagc
     8341 agtgctgttg tcatcaacat cgtcacagtg atcgatagac cgtaagcagc ttccatatga
     8401 tgagacgttc ggaatgtgac aaccactaga gaacaggcga tccataaaat cagattgact
     8461 gctgggatat acatttgccc gatactgttc ccaggataca tgatctttaa ccgaggcagc
     8521 agtttcaact tgattgcttc tgaaactagc gtaaacgaac ctgtaatcaa tgcttgcgaa
     8581 gcaatgactg cagcgactgt tgcaaaagca acgccgatca cgatccaact ttgaggcatc
     8641 atttggaaga atggattcag gttcttgatt tgctgtgctt cagggttttg ataaacattc
     8701 aacaaccacg cagcctgccc aaaataattg ataaccaaac aaattttgac gtaaggccag
     8761 ctcagacgaa tatttcgttt gccgacatgt ccaagatcgg aataaagcgc ctctgcacct
     8821 gtcgtagcca ggaaaatatt tccaagaatg aacaagccta atttgttatc tgcactaaaa
     8881 agcagctgga tagcataata aggattcagt gcacggataa ctgtccagtc ttgactgaag
     8941 ttcattaatc ccattacgcc taaaaaagta aaccaagcga acatgattgg tccgaatgct
     9001 tttccgacaa tatctgtacc aaagcgttgg accatgaata aaacaaaaat aataactaaa
     9061 gtgataatga cgatcacgtt ctggttatta ccgaaccggt caaaaaactg cggaattcct
     9121 cgcaagcctt caatcgcagt cgttaccgtc acagcagggg tcaatacccc atctgctagt
     9181 aaggcagctc ctccgatcat tgcaggaatg atcaaatatt tcccaccttt tcggacaagt
     9241 gtgtagagtg agaaaattcc accttcacca tggttatctg cattcaatgc aatcacgaca
     9301 tatttgattg ttgtcagtag agtcaatgtc caaaaaatca atgagactga tccgataaca
     9361 aaatcaggat tgattcctga caatccgcca ttatcttcaa caatcgattt cataacgtaa
     9421 agcggacttg tcccgatgtc tccgtagacg actcccatcg ctacaagcag gccagtcatt
     9481 gatatctttt tggtattgtg cgttgttgcc acgtttttcc ctcatttctt gttatatgga
     9541 tatcctttag gtaagcttga atgatagatg atattcttat ccatgacacc cgctttccta
     9601 aattgcttct aattatccaa gtaacacact gaagtgtcaa cttatttgaa taaaattatt
     9661 ctttctttgg acgttctctg atacgtcttg ccacaaatga ataaagagca aggcaaaaaa
     9721 ttgcacccaa taatgctggt atcaaataaa aaccgccgag ttccggtccc agtggtccta
     9781 gtaaaaattc gccgagccaa ctacctaaaa aaccgataat gatatttcca gatacgcctc
     9841 ccgggacttc ctttcctaaa attgcgccag ctatcaaacc taatataccg ccgataaata
     9901 acgaccataa aaaattcagc atctcctttc cctcctttat ccatatttct cacttttatt
     9961 ttacgcttta ttcagaagga ctccaaaaat atgcgccaag aaactccaga aaaaaagaag
    10021 ttgtaacaaa agtgttcagc ttatttccgt agaagctgct tctggaacac cgtgaatcag
    10081 aggttgtgaa atcagcggtt tgacctgagt attagagaaa caaacggaaa aatagttcct
    10141 catattttgc cgttttttga gctaatatcg agggacagct gattgaacat cgtttattgg
    10201 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa cagccaaata aaaacagctc
    10261 ctaatgtttt taacttggct ggacttatta ccgcagatgc aagcttttga acaccgttta
    10321 ttcgaaaaaa aaggactgtg acacggcttt tgtcacagtc cccttttttc gaataattat
    10381 tgtccccctg tcaccttcac gatcacaagt acattattcc tatgaattaa attatttttc
    10441 ttcatttttc gttgaaccat cttcgttcaa cattgctttt cttgacagat ttacgcgtcc
    10501 ttgtttatcg atttcagtta ctttcacaag gatctcatca cctagtttaa caacatcttc
    10561 gactttattg acacgatcgt ttgctaattg ggaaatatgg acaagtccat cttttccttt
    10621 gatcaagttg acgaatgcac cgaatttttc gatacggaca actttcccta gataaacttc
    10681 accaactttg acttctttgg tcaaatcttc gatgatcttg atagcttttt tgatcatttc
    10741 tgcatcagct gaagcaatac ttacttttcc atcttgatcg atatcgattt tcacaccagt
    10801 ttcatcgatg attccgttga tcgtatcgcc gcctttaccg ataacatctt tgattttagc
    10861 aggttcgatc tggatcattt cgatttttgg tgcatattgg cttaattctt cacgtggagc
    10921 agcaatcgtt gaagtcaatt cttctaagat ttccatacgt gcttgttttg cttgagttaa
    10981 tgcttcagtt aggatttgtt ctgtgattcc ttgaatcttg atatccatct gtaaagcagt
    11041 gataccatct tttgtaccgg caactttaaa gtccatatcg ccaagatgat cttccagccc
    11101 ttggatatca gttaggatcg tatagttttc accatcagaa acaagtccca tcgcaatacc
    11161 ggcgactggc gctttgattg gtacacctgc atccatcaat gctaatgtgc cagcacagat
    11221 acttgcttgg gaagaagaac catttgattc caatacttct gctactaaac ggatcgtata
    11281 aggaaattct tcttctgaag ggattacttg tgctaacgca cgttcaccta atgcgccatg
    11341 tccgatttcc cgacgccctg gagagcctgc acgtcctgta gaaccaacag aaaattgagg
    11401 gaaattgtag tggtggatga atcgtttgct gacttccacc cctaaaccat caatgatttg
    11461 gtgctctcct agaggtgcga gtgtacaagc tgataaagct tgtgtttgtc ctcgtgtgaa
    11521 caaacctgat ccatgtacac gtggcaataa gctgacctct gatgacaatg gacggatttc
    11581 atccaattta cgcccgtcag gacggatctt atcgatggtg atcaattcac gtacgacatc
    11641 tttttcaaga tcttctgtga tttgtttaac ttctttcaac aagtgatctt catcttcatg
    11701 acctgcaaat ttctcagcat aagcttcgcg gatatcgatc ttcacctgct cgatattatc
    11761 ttcgcgagca agtttttctt cagtcattac tgctgctttc attgtagcgt aagaagcatc
    11821 aaagatttct tttttcaata cttcatcaac ttgcagtaaa gtcacttcca ttttttcttt
    11881 ccctactgct tgaacgattt cttcttggaa agcaactaac tctttgatag cgtcaaaacc
    11941 aaacagcaat gcaccaagca tgtcttcttc tgaaacttct ttcgcgccgc tttcaaccat
    12001 attgatggct tgtttcgttc cggcaacagt caattcgata tctgtttgtt ctgcttgttc
    12061 tactgttgga ttcaagacgt actcgccatt tacacgacca acttctacgc ctgcgattgg
    12121 gccgtcaaat gggatatctg agattgatag tgctaaagaa gaacctagca ttgctgccat
    12181 agctggcgag cagtctgttt ctacactcat cacgatgttc gttacttgga cttcattacg
    12241 gaagccttct gcaaacatcg ggcgaattgg acggtcgatc aaacgggcag tcaatgttgc
    12301 ttccgtactt ggacggcctt ctcgtttgat gaaacctcca gggattttcc ctgctgcata
    12361 cattttttct tcataattga tcgttaatgg gaaaaagtct gtatcttttg cttctttaga
    12421 ggccacagct gcacttaaga ctactgtatc tccataacgg actaaaacgg ctccgtttgc
    12481 ttgttttgct agctgaccga cttcgatttg taagggacgg ccgccccatg tcgttttgaa
    12541 tacttgtttt tctgacataa cttctccttt tccttctcgt gaaagcaaga cgctactaaa
    12601 caaatgaaaa ctgccgaaag ccgtcttttg agcagctttc atttggttag taggagtcca
    12661 tctagcttga tcacggaatt ttacttaatt gatatacgag taaaaaaagt gagattcatg
    12721 tgaatcccac tctttttgac taaaattaac gacgcaagcc taaacgtttg atcaattcgc
    12781 ggtaacgttg aacgtcagtt ttacgtaagt aagctaacaa gttacggcgg tgaccaactt
    12841 ttttcattag gccgcggtat gaatgatggt cttttttgtg cacgcgtgca tgttcattca
    12901 agtggttgat ttcttcagtt aatacagcga tttgtacttc tggagaacca gtatcccctt
    12961 catggcgagc gtattcgttg atgatttcat tcttgcgttc ttttgaaatt gccatttctt
    13021 tcacctcttt cgattattcc cctgcgactg agtaaaacgt tggtgattcg cttaaccaag
    13081 tggcaggtca tgtgcctaga gcacacagct atactttacc tgaaaccggg ttgaaaatca
    13141 agtagttaat cattaagaaa aggtagtttt caaacaatct tactactact tttccatttt
    13201 tgtggtaaac tataatgcga aaatagactt gaaacacagt cttttaagaa aggggtacat
    13261 attccatgat tacaatggat gatatcattc gtgaaggaaa tccgacctta cgcgaagttg
    13321 caaaagaagt ttctcttcca ttaagcgaag aagatatctc tttaggaaaa gagatgcttg
    13381 agttcttaaa aaacagccaa gatccaatca aagctgaaga actacactta cgaggcggtg
    13441 ttggcttagc tgctcctcaa ttagatattt ctaaaagaat cattgctgtt cacgtaccaa
    13501 gtcctgaccc agaggcagat ggaccttctc taagtactgt tatgtataat cctaaaattt
    13561 tgagccattc tgttcaagat gcttgtttag gggaaggtga aggctgttta tctgttgatc
    13621 gagaagtacc aggatatgtt gttcgccatg ccaaaatcac tgtgtcttat tatgatatga
    13681 atggtgaaaa acacaagatt cgtttgaaga attatgaatc gatcgtcgtt caacatgaaa
    13741 tcgaccatat caacggtgtg atgttctatg accacatcaa cgatcaaaat ccatttgcac
    13801 taaaagaagg cgtattggtg atcgagtaga gaattggcag gttccttcga tactatgcta
    13861 caagaaagct ttataaaaaa ggggagtgct caaaagctgc acttcccttt tttatgtttc
    13921 tccactaatc gaaatttaga tagacatcta gaaatcgcaa ttccaaaaaa ttctgtgtat
    13981 tggatacgca tcttttctta ttttttctta tttcttccat atgggtgttt atctcagaga
    14041 tgatagaata gtttctagga taaaaagtag cagctacgat gttatccttc gtagaggatt
    14101 cctgcttcag ctagtttttg tttgatgttt ttcagcatat gcttttctgg gtagcggatc
    14161 gtatgcagat ggatgccgcc tgttaattcg gataacaaag tagcattact tttttgataa
    14221 cgtctcataa aggaatcgat atcttgtggc gttttgatgt gcaaagttcc tgctaactca
    14281 ccgtaaaggg gatgttccac aattacatca atgatttcac caccattttc aacgattagt
    14341 ctcagttctt cttctgtttg atcttttcca tgctggacag cgattttact gatgtgccca
    14401 gtattggttt cttctgtttc gaggatataa cctcttgctg ttgccacgat acttttacct
    14461 gcagctcgta gtaaagcaat atcgcctaca atgatctgac ggctgactcc aaaagttttc
    14521 gcaaacttgc tagcactcac tggtttttct gtatttgcca aggtttgcat gatttgtttt
    14581 cgccgtgtct ctccttcgat cacacttatt cctccttcat tttgttttgg tattcgcgcg
    14641 agtgtttcat caaatcaaca tacgtatctg ttattgtttc cttatatccc ttatttttca
    14701 gcagactaac aactttatcc aatacttgct gctctctttt tgcatcataa actgcttggt
    14761 tttcttcttt tttcacttgt cctattttag ttaccgtatc cattcttttt tctaataaag
    14821 caatcaactc tcgatcgatc atatcgatct tatgacgtaa tgtttcaaga gactcttctg
    14881 ttagttcttc ctctactgtc ttgatgatac gagcaggatt tcctgcaatc actacattgt
    14941 cgggaaaaga tttggtcaca accgaacctg cgccgactac tacgttgtcc cctagagtga
    15001 cacccggcgt gacgataact cctccgccta accaaacatt attaccgatt ttaatgggtt
    15061 ttgcgtactc cagaccgctg ttacgtttca ctggatgtag tggatgcgtc gccgtgtaaa
    15121 gctggacatt cggagcgaac atgcagttat caccgatttc aatcgtgctg acatctaaaa
    15181 atgtacaatt gaaatttgca taaaaattct ccccaacaaa tatattatat ccgtaatcaa
    15241 aattgataac tggctccata tagatttttt tccctgtacg gccaaaagtc tctttcaaca
    15301 attgggatcg cagttccgct gattctgctt ggttgatgat ttggctttga cttcttgcaa
    15361 acatacggtc ggcaaccagc tcaggatcat tggcaaaata aagctcgcct gcaatcattt
    15421 tttctttttc ggttttgaat tcagtcataa tctgctccta attcttgtat ttttttgaaa
    15481 acaaccgctc cgtcttcttg aataaacgta taaggctccg ctaaataaac aggcgtttga
    15541 ttgattacta agattttggc tgacggctgg cgaaactgta ccagatcaca aaacggatga
    15601 acttgaaaac tcgttccaac aatgacgatc agctctgctt ggctgacggc ataagccgcc
    15661 tgctccatga ctccatcttg aaacccttca ccatataaca cgatgtctgg gcgtatttgt
    15721 cctccgcatg cttgatgttt gtcgctgtga agatattctt tccagtctac cgtttgttga
    15781 cattttcgac aatgacattg atacaaactt ccatgaaaat tgacgagttg cgggctttta
    15841 gcctttgcgt gcaaaccgtc gatgttttgg gaaacaaccc aaacattttt ttctttcgct
    15901 agatgtgcca tttccaaatg aatgatattg ggttttgctt caggatgata aatctttttg
    15961 acaaattgat agaatttatc cggttcattc accattgctt gatgactcag caagtattcc
    16021 ggctgctcca tcccgtggta gattcctgac aaagatcgat agtctggcac tccagaaggt
    16081 gtggataccc ctgcaccggt caaaaatgta atttgctgat aagctaatat ctcttttgct
    16141 gcttcgtcta ctgttaattg tttcatgttt tgtcacctca ctttccatta tagcccatac
    16201 tcgaacaaat ttattgttga aaagtcagaa aaatgggaat aaaaaagtgt tatgggcatt
    16261 tgaacaattt atcacaatgt tcactgacaa aagaatgaaa acgtgttaca atactgctgt
    16321 ataaaagtga ggtgaatcga tatgttacca ttctttattt attggggcgt tattctggct
    16381 tgtatcgcct ggttagcgtt aagcgtttat ttctctgttt tttatcttgt acgcaaagaa
    16441 aatggaaatc tttgggcttt tgctttattc aacgtgattg ccgcaattgt tttagccatt
    16501 acattagccg tatatcgaac atggggctgg ggaatcaccc aatactcaag tttgatttat
    16561 ctaatattag ccatttatgg cgtcgttgtt cttttgcaag cgatcctagg acgtgagcca
    16621 aagaaagcgg cagcttaaat gatcgagatg gaactggaca caggaaattt tctgttctag
    16681 ttcttttttt attgtattac atacattttt gtgtataatt atactatcgt ttgcaatgga
    16741 tagatgattc acattttgat tgttttttcg gtaaaagtaa agatttctat atttatcagt
    16801 attttttcaa aaaacagcag ttatagattg acaaagcctc tcgagtatcg taaacttttt
    16861 ttgtataaat aactgaatac gagatgcctc aatcaatgag gtagaggtcg cgagaatcat
    16921 taaccctgtg gagtgaagca aacatggaga agcaggaata gggaggatcg ccgaaatgtg
    16981 cacttttttg cttggagtgt atgttgggac gcaagttaag agcttgcgga ctgtcttagc
    17041 agtgatgcta agttgcgcta tgttttgtga gaggtttatc accttatgga attttagcgg
    17101 cctttttata tctataaatc ggtcgctttt tattttttca gataaagctt taccgcattt
    17161 catctagacc gctcgtgtat catataatca gaaagggaga aaaaaatgaa aaaaaatatg
    17221 gtttcagttg ccgtactatt tgcttttgtc ttctcactat tcagtgtggg atctctggca
    17281 aatgcagatg aaaaaacacc gagcgatgag tttcgcgtag gtatggaggc tgggtatgca
    17341 cccttcaact ggtctcaaca aacagatgag aataatgcct tgccaattca aggccaaaat
    17401 agttacgccg gcggttatga tgttcaaatc gctaaaaaag tagcagatgg tttagggaaa
    17461 aaattagtca tcgtccaaac gaaatgggat ggtctggctc ctgcccttca atcaggcaag
    17521 atcgacgcga tcatcgccgg aatgagccct actgctgaac gacgaaaaga aattgatttt
    17581 accgatcctt attacgaatc acaattagta gtagtagttc aaaaagatag caaatatgca
    17641 aaagcaaaaa gtttagcaga tctttctgga gcaaagatta ctgctcagtt aaatacattc
    17701 cactatactg tgatcgatca gataccagat gtgcagaaac aacaagcaat ggataatttc
    17761 tcagcaatga gaacagcttt agcttctgga atgatcgatg gttatgtcag cgaacgtccg
    17821 gaaggtgtaa cggcaactag tgtgaacaaa gacttggaaa tgcttgaatt cagcggtgca
    17881 gatggcttcc agacaaaccc ggaagatgtt cagatttctg ttggtatgcg taaaggcgac
    17941 ccggatatcc aaaaagtaaa ccagattctt tctggcatct ctaacgatga acgaaccaaa
    18001 attatggacc aagcagtcaa agatcagcct gctgcaactg atagtgaaga aaaacctggc
    18061 atcatttctg atttcactac gatttggaaa caatacggca atatgttctt acgaggtgct
    18121 ggacttacct tgttcatcgc attgatcggg acagttgtcg gtactacact tggattactg
    18181 attggtgtct tccgttcggt acctgaatca ggaaatgcac tgactcgctt cttccaaaaa
    18241 atcggaaatg cacttctttc tatctatgtc gaagtattcc gcggaacacc gatgatggta
    18301 caagcaatgg tcatcttcta cggtctggca ctcgcttttg gcatttcatt gaaccgaacg
    18361 attgccgctt tgtttattgt ctctctcaat actggagctt acatgacaga gatcgttcgt
    18421 ggggggattt ttgcagtaga tgaagggcag ttcgaagctg cgcaagccat tggtatgacc
    18481 catggccaaa cgatgagaaa agttgttatt ccacaagttt tacggaatat cttgccagca
    18541 acaggaaatg agtttgttat caatatcaaa gatacagcag ttttaagtgt aatcggcgta
    18601 gctgatttgt tcttccaagg aaactctgct tccggtgcga atttccaatt cttccaaaca
    18661 tttacgatcg tcgggatcat ctacttagtc atgaccttta cgatcaccag aatcctgcgg
    18721 gcggtagaaa agaaaatcga tggtccttca gcttatacca agcttgaaga aatcgacaat
    18781 tcaaatttgc aggaataagg aggaagaatc atgaatgaaa aaattattga aatcaaacat
    18841 ctaagaaaaa gcttcggtga aaatgaagta ttgaaagata tcagcgtaac agtcaataaa
    18901 ggtgaagttg tgacgatcat cggttcttcc ggttctggta aatctacttt cttgcgctgt
    18961 atcaatttac tggaaaaacc aacaggcggc gagatcattt ataatggaga aaatgtatta
    19021 gctcctaaat ataatttacc taaatatcga acgaatctag gtatggtttt ccaatccttc
    19081 aacctgttca acaatatgaa tgtcttggaa aactgtatgt ctggtcaaac aactgtcctg
    19141 aaacgagata aagaaactgc caaaaaagta gcaatcgaaa atctggaaaa agttggtatg
    19201 gatcgttata tcgaagccaa accagcacaa ttatctggtg gacagaaaca gcgtgtcgca
    19261 attgctcggg cattatcaat gaatccagat gtattgctat tcgacgaacc tacttcggcg
    19321 ttagaccctg aaatggttgg cgaagtgctt aatacgatca aagacttagc acatactggc
    19381 ttgacaatgc ttatcgtgac gcatgagatg gaatttgcaa aagatgtatc tgaccgtgtc
    19441 atttttatgg ataaaggtgt gattgcagaa gaaggtacac cagaagatat ctttgtccat
    19501 ccgaaagaag aacgtacaaa agaattcctt tcacgtattt taaatgctta ggatcaatag
    19561 ctgattggga caaagagctg cggtgtttac cgcagttctt ttttatgaat tactcataaa
    19621 aggtaaattt ctgaaaattt tggaatctta gttgtgtttt tcagaaaact cttttaatat
    19681 ggaagtaatt aaactttttt agttaggatg atttaaaaat gaatgaagag caagaactgc
    19741 agcgtggact gaaaaataga catgtccaac tgatttctat tggcggagcg attggaacgg
    19801 ggttgttttt aggttccgga aagtctatcc agctagctgg cccttcgata ttgctcgcct
    19861 atcttattac tggatgcatc tgttttttca ttatgcgtgc gttaggcgaa cttctgttat
    19921 caaacaccaa taaccattct tttctggatt ttgttgcaga atatcttggc aaaaaagcgg
    19981 ctttcatcac tggctggacg tattggttct gctgggtatc gattgcaatg gccgatttaa
    20041 cagccatcgg aatgtatgta cgctactggg cgcctggtgt tcctcagtgg cttcctgaac
    20101 tgattgcatt aggtcttctt ctttgcttga atatggtggc tgtctcattg tttggtgagt
    20161 tagagttttg gtttgcattg atcaaagttg tagcgatcat tgcctttatc ctcgtaggag
    20221 cttatatgat cctcacccac tatacgacag atattggcac agccagtatc acgaatctat
    20281 ggtctcatgg cggattcttt ccaactggtg caaaaggatt tatcttagct ttccaaatgg
    20341 taacctttgc ttttgctggg atagaattag ttggattagt cgctggagaa acagaaaatc
    20401 ctgaaaaagt gttgccggaa gctatcaata atattcctat caggatcatc ttattttact
    20461 taggatctct ttttgtcatt atggcaatct atccatggaa cagcttgaat ccagataaca
    20521 gtccatttgt agaagtattt tcggaaattg ggatcacgat tgctgcttcc ttgatcaatt
    20581 tagtcgtatt aagtgcagca gcttctgcct gcaacagtgc aatatatagt acaggacgta
    20641 tgcttcgttc tttagcgcaa gaaggcagtg cccctaaaaa attcaaaaag ttaacaacac
    20701 atcatgtgcc agggaatgct ttgacatttt ctactatcgt cattttcatt tctgttatct
    20761 tgaattacgt gatgcctagt gaagtattca cactagtaag cagtattgcc actacttgtt
    20821 tcttgtttat ctggagtatg cttgtctaca cgcacatgaa atatcgtaaa agtatcctcg
    20881 gcaaaaaagc tcattcattt aaaatgccgt tgtacccatt tagtaattat ctggtatttc
    20941 tttacatggc ctttgtgtgt gtcgtcttgt tcctaggaaa agatacgaga atcgctttat
    21001 tactaacacc tgtgtggttc ttgcttctat tactaatcta tcatatgaaa tatgaacgta
    21061 agaagtaaga aagtagttgt gacagaagtg gtcagcttcg agaaataaga aggaatttac
    21121 gaaaattgct tcttaaattt ttgtgaaatt tcggcttatt tcttggagtt aaaagcttct
    21181 gtcccagcct cttttctatt tccccggaaa caaccgctga taataggcat ctgctttttt
    21241 ataggaaaaa atccaaatac cagatacaaa aaaacgtccg aataacccga tcaacggtac
    21301 aaaaaagaca ccggcaatca agcataagtt aatcatcatg gagataagac ttcgacggtc
    21361 ctttgacaaa actctcacga catgggaatc tggtttattt gctttcatga tttgcctcat
    21421 caagatacca aaagaaaaac tccacatcgt gtatactaag acataagcca tctcaggcca
    21481 aaaagcttca gggttacgcc cgacccaact agtaacagaa ggacataaag tcagccaaaa
    21541 gagcaaaaag atattcgacc agaccactcg gtaattaatt cgttctgcca tctgaaatat
    21601 acgatgatgg ttcacccaga tcaccgcaac aaatataaag ctgatggtat aagctaataa
    21661 tgtatttcgg atgacccata aagaatgaaa gctagatgta ttagggatct tgatctctaa
    21721 tacaagaact ctcaaaacaa tggctaccac tccatcagta aatgcttcta atctcgactt
    21781 tgtcacgatg attcctcctc agatgattca gcgcttttat tttatcactt acgttattaa
    21841 ataaccgtcg ttttatatag gaaattttct gaaaattgtt gcatattttt cgaaattttg
    21901 ttacttttaa aaaaaccgta aaggagttat gtatgacaaa aaaattatca tttaaagact
    21961 atctttttat aggctccatg cttttcggtc tattttttgg tgctggaaat cttatctttc
    22021 ccgttcattt gggtcaagaa gcaggcgctg ctaccttttg ggcgaatcta ggtttcttgg
    22081 ttaccggtat cggtcttccc ttcttagggg tgattgccat tggtgtatcg aaaagctcag
    22141 gtgtatatga attagcaaaa cggataaatc aaacatatgc gatggttttt accatcttgc
    22201 tttacttagt tatcggaccg ttttttgctt tacctcgcct tgcaaccaca tctttcgaga
    22261 ttggtttagc accatttatc gatcctagca gacagacgct atatctagca attttcagta
    22321 ttgtattttt tgtgctggct tggtggtttt caagaaaacc gacaaagata ttagattatg
    22381 ttggaaagtt cctgaatcct gcatttttgg ttttattagg tgctttgctt ctccttgctt
    22441 ttttgaatcc actaggttct gtaaatcaag cgcctattca gccaaattat caagagcatg
    22501 cttttttcac agggtttact caaggatata acacattaga tgcgcttgct gctttagcat
    22561 ttgggatcat cattgtcaca acaatccaaa acatgggtgt aacaaaacct gctgaaatcg
    22621 ctaaagacac gatcaaatct ggtgcgatca gcattatatt gatgggaatc atttatactt
    22681 tgctcgctta tttaggtgct atgagcttag gtagctttgc tctaagtgaa aatggcggga
    22741 tcacgctggc tcagattgct gatcattatt taggtactta tggcagtatc ttattagcat
    22801 ttatcgttat attagcttgc ttgaagaccg gtattggttt gattacagct tttgcagaaa
    22861 cattcacaga tttgtttccg caaagaaatt acgcattctt tgttgcactt gctagtatcc
    22921 ttccttgttt agcggcaaac gtaggcttga cgaatatcat tcaattttcg ttgcctgttt
    22981 tgatgttcat ctatccatta gcaatgacac tgattttact agtgttagtt ggtcctttgt
    23041 ttaagcagcg tacggctgtt tatcgaatga ctacttattt tacattgatt gcttcgattt
    23101 ttgatgggtt gaacgcttgc ccagaaacta tcaaacaaac acctattgtt caaaatcttt
    23161 tatatgttgc ggaaagctac ttgccgtttt tcaaattggg gatggggtgg atcgtaccag
    23221 cagttatcgg ttttgttatc ggactgattt ggagtttcgc taagaaagaa gaagtagcag
    23281 attgataaaa gaggttgtga aagagacgtt cagccttatt gccgataggc tgtctcttga
    23341 acactgttta ttgagaggtc gtgaaaaagc cgctcagttt cgagtattaa gaaatatttt
    23401 ttgaaaatgg cttctcacat ttttgcataa atttgactta ataccgagaa actggctttt
    23461 gaacaccgtt tattgagcgt ctataaactg acctgagtat tagagaaaca aaacggcaaa
    23521 gtagctccat atactttgcc gttttgtttt gaactaatac cgaagagaag ctgatcaaac
    23581 aacatttctt ttataatata gattttagac atatatatga tataaaaata gttttcgcaa
    23641 aaaaacttaa caactttcga aaactttcgt cacaaaatat tcacaactag tgactattat
    23701 ataagcatac caacaaacaa ccctaacaac actttttcat ttttaactcc ttatcctgct
    23761 agctccccgg ctagcaggac ctttttttat
//
LOCUS       DACDUU010000042        23717 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.042, whole genome shotgun sequence.
ACCESSION   DACDUU010000042 DACDUU010000000
VERSION     DACDUU010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.042"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(55..759)
                     /locus_tag="IWL30_001671"
     CDS             complement(55..759)
                     /locus_tag="IWL30_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HAP9706428.1"
                     /translation="MFAENLKFLREKYNIDQQELAEKLGRKSSSSISEWEKGKYTPKL
                     KTLNEIAKIFNVDIDDLMNIDLSISNQIYNILPIYNQLNDENKVATYEFASNRLEEQK
                     QENVLDFPKKEKIPTVHNSASAANPTELTYGDTVVEEEEFERVPSAADFAVPIIGDSM
                     EPVIRNGQFVFVKEQPDVEDGEIAIVELGGDGVTCKEIYKDYENQTIILRSINDLYED
                     RIVSPEQIRIIGKVVF"
     gene            959..1225
                     /locus_tag="IWL30_001672"
     CDS             959..1225
                     /locus_tag="IWL30_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9706429.1"
                     /translation="MDTAKKEKYTLRQLRALKGYSKEELSRKSKVTSRTIFIYENDVD
                     KMRNGKYATLEKIAKALGVRVSDIFLDPDSEKPKSITGETKQVS"
     gene            complement(1393..1623)
                     /locus_tag="IWL30_001673"
     CDS             complement(1393..1623)
                     /locus_tag="IWL30_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP9706430.1"
                     /translation="MGKNQWVSPRNGKWAVRGEGNSEDTKLFDNKSDAVEYGKKISKK
                     QQSEFIVQKKNGQIQSKDSFGNDPIPPRDKEH"
     gene            1695..1850
                     /locus_tag="IWL30_001674"
     CDS             1695..1850
                     /locus_tag="IWL30_001674"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706431.1"
                     /translation="MSNKPKITLNGTPNLQRLADEMARIRSRHWGVTVVATIIEQKEK
                     PTQPASK"
     gene            complement(2159..2383)
                     /locus_tag="IWL30_001675"
     CDS             complement(2159..2383)
                     /locus_tag="IWL30_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004401476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP9706432.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            2650..2985
                     /locus_tag="IWL30_001676"
     CDS             2650..2985
                     /locus_tag="IWL30_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706433.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            3218..4159
                     /locus_tag="IWL30_001677"
     CDS             3218..4159
                     /locus_tag="IWL30_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAP9706434.1"
                     /translation="MSKSTLEMSHQEWLEDRKRGIGGSDVATVLGLNKYKSPYQLWLE
                     KTGQIELKDLESEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPVIDGSGATADFLKEKYADVEENQTA
                     LPSRFDELIEQKRELKRTKKEIESSIRQVDNEIISELGKREASIGITQRNIISWKLVR
                     TRRMNTKKLAEKYPDVANDEEIYNVTESRRLTEKEIK"
     gene            4161..5051
                     /locus_tag="IWL30_001678"
     CDS             4161..5051
                     /locus_tag="IWL30_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HAP9706435.1"
                     /translation="MATNESLKNQLAEKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            5094..5894
                     /locus_tag="IWL30_001679"
     CDS             5094..5894
                     /locus_tag="IWL30_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP9706436.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWSLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            5909..6760
                     /locus_tag="IWL30_001680"
     CDS             5909..6760
                     /locus_tag="IWL30_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9706437.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            6757..7074
                     /locus_tag="IWL30_001681"
     CDS             6757..7074
                     /locus_tag="IWL30_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706438.1"
                     /translation="MNLTKQEKRICRQIMLQIIGDRDMDELSQQESRMVRKISKLIGA
                     DTMDRSKPLPKLNFESFTFEKYSHLREVGYSVNAIRKALGVGSSVWAKWRKENVNEVN
                     QND"
     gene            7067..7249
                     /locus_tag="IWL30_001682"
     CDS             7067..7249
                     /locus_tag="IWL30_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706439.1"
                     /translation="MIKLIGKETLKVYYVAETKAEVSRWLLKMFNKTRANASVSQKAS
                     VSETGMPEAMWISRRN"
     gene            7251..7721
                     /locus_tag="IWL30_001683"
     CDS             7251..7721
                     /locus_tag="IWL30_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706440.1"
                     /translation="MAMIPKFRAWDREKDRMIYPSTTGVCFEMNDDGISVLDIDVDYP
                     NDYGFPKIASILMQSTTLKDKNGVEIFEGDIALITHKSTNYADTYWHSYVEVFRAKNG
                     AYRIRGEHIYETELYSNRKQLTVAGNVYNSPELLKRDRFMTTTENPELLKEEQR"
     gene            7718..8260
                     /locus_tag="IWL30_001684"
     CDS             7718..8260
                     /locus_tag="IWL30_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP9706441.1"
                     /translation="MNKQELIDKYTAEISRLRPYCPNRHLISDQLKYGLYKEILEDLK
                     QLDEPQKPAVPKFVAEWIEYAKKKGDSLAISFKPWNLYGVEYSKTDRWIEDNQGTFAR
                     AWLIGYEVEKEPLYYVKLPVVYFNHWDLETYLMKDDRGNITIADNNDFDDMKFTESEI
                     KAIDERYWPFAVPVEEVAEG"
     gene            8257..8640
                     /locus_tag="IWL30_001685"
     CDS             8257..8640
                     /locus_tag="IWL30_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706442.1"
                     /translation="MSNLKVGDIVFYKGDEGIIREVMTDGKENFYRVDVSRKSILYLY
                     EDELDLGTQKLNDNQKMVFDYLKSNVEQGGKSVMYSIFLLGDWDSRIGAARNVDIAYC
                     GLNGKQEAEVLSAFAQWGLEQEEAE"
     gene            8637..8867
                     /locus_tag="IWL30_001686"
     CDS             8637..8867
                     /locus_tag="IWL30_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706443.1"
                     /translation="MKYEIPLSEADVQSIINGREVNKKLPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSIDDTFRLGS"
     gene            8874..9077
                     /locus_tag="IWL30_001687"
     CDS             8874..9077
                     /locus_tag="IWL30_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HAP9706444.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASNAIRKFGISILNDDRKWEEMR"
     gene            9074..9370
                     /locus_tag="IWL30_001688"
     CDS             9074..9370
                     /locus_tag="IWL30_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706445.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWTAKSKENLAVVRLVDIELINDK"
     gene            9447..9860
                     /locus_tag="IWL30_001689"
     CDS             9447..9860
                     /locus_tag="IWL30_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP9706446.1"
                     /translation="MQLLREVAFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            9873..10259
                     /locus_tag="IWL30_001690"
     CDS             9873..10259
                     /locus_tag="IWL30_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706447.1"
                     /translation="MWKDYVSLKELKKDLIFKKIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHSSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKEKLCVVTDA"
     gene            10751..10823
                     /locus_tag="IWL30_001691"
     tRNA            10751..10823
                     /locus_tag="IWL30_001691"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10784..10786,aa:Lys,seq:ttt)
     gene            11209..11469
                     /locus_tag="IWL30_001692"
     CDS             11209..11469
                     /locus_tag="IWL30_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706448.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSVFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            12074..12907
                     /locus_tag="IWL30_001693"
     CDS             12074..12907
                     /locus_tag="IWL30_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small subunit of terminase"
                     /protein_id="HAP9706449.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELKSNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFETLSDEEKDIYSSLNDNPSFVLSEEIRLLKIRQFRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKNPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            12900..14315
                     /locus_tag="IWL30_001694"
     CDS             12900..14315
                     /locus_tag="IWL30_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase B"
                     /protein_id="HAP9706450.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPENMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTTVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            14327..15856
                     /locus_tag="IWL30_001695"
     CDS             14327..15856
                     /locus_tag="IWL30_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP9706451.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKDPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            15948..16826
                     /locus_tag="IWL30_001696"
     CDS             15948..16826
                     /locus_tag="IWL30_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAP9706452.1"
                     /translation="MMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            16828..17145
                     /locus_tag="IWL30_001697"
     CDS             16828..17145
                     /locus_tag="IWL30_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706453.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            17194..17895
                     /locus_tag="IWL30_001698"
     CDS             17194..17895
                     /locus_tag="IWL30_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HAP9706454.1"
                     /translation="MVEWWGATIKTQSNAGRENESWGERRMSMKHKSLMPMNLQYFAE
                     GDDQKFSFDDFKSFVESNEEAQKFIQSQSQSVADKQLEAWKQNNLDKIKQDTIKEYEE
                     SKKNKSPEQIQLEKLQAEFEAEKALRVTSDNKAFVAEQIAGLELDGELKESISQFMLN
                     NLVSSDTDFTKNAVEGFTSVLNAINEKHADALKELQMKSAFGGTQQSNNQVQQNNETL
                     TNPEEQLGQILQQFN"
     gene            17910..18800
                     /locus_tag="IWL30_001699"
     CDS             17910..18800
                     /locus_tag="IWL30_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HAP9706455.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLFGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            18823..19038
                     /locus_tag="IWL30_001700"
     CDS             18823..19038
                     /locus_tag="IWL30_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706456.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            19050..19391
                     /locus_tag="IWL30_001701"
     CDS             19050..19391
                     /locus_tag="IWL30_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706457.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            19391..19762
                     /locus_tag="IWL30_001702"
     CDS             19391..19762
                     /locus_tag="IWL30_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706458.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            19755..20150
                     /locus_tag="IWL30_001703"
     CDS             19755..20150
                     /locus_tag="IWL30_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAP9706459.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            20152..20529
                     /locus_tag="IWL30_001704"
     CDS             20152..20529
                     /locus_tag="IWL30_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706460.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            20530..21138
                     /locus_tag="IWL30_001705"
     CDS             20530..21138
                     /locus_tag="IWL30_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP9706461.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            21138..21479
                     /locus_tag="IWL30_001706"
     CDS             21138..21479
                     /locus_tag="IWL30_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706462.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            21721..>23717
                     /locus_tag="IWL30_001707"
     CDS             21721..>23717
                     /locus_tag="IWL30_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HAP9706463.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANSFVLIIGAVVGAI
                     AVFGYFMATNEEFRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNEIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFA"
BASE COUNT         8509 a         3488 c         5022 g         6698 t
ORIGIN      
        1 gtcaaaatca tataaaccct taccgaagtt gacggctccg gcatgggaat ttttttaaaa
       61 tacaactttt ccgataattc taatctgttc tggcgataca attctatctt cgtataagtc
      121 gtttatagaa cgtaaaatga ttgtttgatt ttcgtaatct ttatatatct ctttacatgt
      181 aactccgtca ccacctaatt ccacaatagc aatttcccca tcttctacgt ccggttgttc
      241 tttaacgaat acgaattgtc cattcctgat aacaggttcc attgaatcgc ctataatagg
      301 tactgcgaag tcagcagcac ttggaactct ctcaaattct tcctcttcga caaccgtatc
      361 accgtaagta agttctgttg gattggcagc cgaagccgaa ttgtgaacag ttgggatttt
      421 ttctttttta ggaaaatcta ggacattttc ttgtttttgt tcttctaatc gattacttgc
      481 aaattcatag gtagcaactt tattttcgtc gttaagctga ttatatatag gaaggatatt
      541 atatatctga ttagatatag ataaatcaat attcatcaaa tcatctatat caacattgaa
      601 tatcttagct atctcattta aggtttttaa ttttggtgta tattttcctt tttcccattc
      661 gcttattgag gatgaacttt ttcttcctag tttttcggct agttcttgtt gatcaatatt
      721 atacttttct ctaagaaact ttagattttc agcgaacata gtaacacctc cttgagttaa
      781 ttgtaacacc gtttcggaaa aaatgtaaca ggtttcttag aaaatatttt agtgttttta
      841 aagaagtatt tacgcaaaat agaaatcgat ttcaggaaaa ctgaaatatg cacttgactt
      901 cggaaaaacc gaagtatata atttagtcag aaagttaaag agaggaggcg aataaaatat
      961 ggataccgcg aagaaagaaa aatatacatt gagacaatta agagctttaa agggttattc
     1021 aaaagaagaa ctgtcaagga aaagcaaagt gacatcaaga acaatattta tttacgaaaa
     1081 cgatgtagat aaaatgagga acggaaagta cgccactcta gaaaaaattg ctaaggcatt
     1141 gggtgttaga gtttccgata tttttttaga ccctgattcg gaaaaaccga aatcaataac
     1201 aggtgaaaca aaacaagtca gctaggaggt gtgaatagtg gaagagaaga atataaaagt
     1261 accgactctt gaagaatata caaaaattgt tgtcgaaaca gacgagcaga acccgaaaac
     1321 aatcgcagtt attacggcga atgatattga atcatgcgaa ggttttcgag ttcgaatgac
     1381 ccctagatat gattaatgtt ctttatcacg tggaggtata ggatcgtttc caaaactatc
     1441 ttttgattgt atttgtccat tctttttttg gacaataaac tctgattgct gttttttaga
     1501 aatcttttta ccatactcaa cagcatctga tttattatca aatagttttg tatcttcgga
     1561 attaccttca ccacgtacag cccatttgcc atttctagga gatacccatt gatttttacc
     1621 cataacaatt cacctcgctt tcaagttaat tctattaaga ggtgaaaaga aaacaaactg
     1681 atggaggtga gattgtgagt aacaagccaa aaatcacttt gaatggcaca cctaatcttc
     1741 aaagattagc agatgaaatg gctagaatac gttctagaca ttggggagtt actgtggtgg
     1801 caaccataat tgaacaaaaa gaaaagccca cacagccggc aagcaagtag tgggcaccat
     1861 agaacaataa tattatatca cacactcgcc acttccgaaa agaggagttg ctgctctcct
     1921 ttcaagtttg ccaaagtcca cgtttcttca ttttggcaac tcctcttctt ggaggtggtg
     1981 acggcgagag acgaaggagg gatgacatga aggaagaatc aaaaataggg attttgaatg
     2041 agaacaatta cacaagatta attattgaaa cagaagaagg ggaaaaagta gcagaagtta
     2101 cattgaccga tgcaactcct gctgacggtt atcgtattcg attaactcct aaatatgatt
     2161 aaccttttgg tgggtgtgga tcgtttccgt gactatcacg gctttgaatt tttccatctc
     2221 tgccatgaat aaatagctca gatccttgat ttttagaaat ctctctagcg atttttactg
     2281 cttctgattt tgtattagta tgtgcagtcg ctttagaatt tccagcacct ttgacattcc
     2341 aaccaccatt ggaattaggt acaacatgtt gattttttcc catagttttc acctccattt
     2401 ctatagaaat attttatcaa atagaaatgg agaaattcaa taaacgaaag gagggatcaa
     2461 atgacattac aagagcatca acacttgatg cgaaaactaa atcaggaata ccacgaggaa
     2521 atgaaaccta gattgctagg ggaacaatgg cgtgaacgcc agaagaagtg gttagaaata
     2581 aaaaagtgac tcagccgtcc aaagcaatga gtcacaaacc aaaaattata cctaaggaga
     2641 ttttatcaca tgaataagaa aattgaaaat cttatcgaag aattgaaacg tgaatgccaa
     2701 aagcaaggtg tttctattat ttgtacagcc caaaaagaag gggaacttaa aagtctcgtt
     2761 tatggtgaga caacagaaat tttactttgt cttgctatgc aagaagaaca tttagatgaa
     2821 aatctcccat tgtcagcaca tattatgcgt agaattgctg tggatgctta cgaacaagcg
     2881 aaaaatgaag aagaaaatca accttcaaat catacttttg ttattaataa caaggaagat
     2941 ttagctgatg tgatgactcg tattctcaag ggggaatttc aatgatgaat gaaaaaacaa
     3001 ttgatctact cattatttta tttttcacgt tagtgattgt agcgaaaagt tatgtaatct
     3061 ttggcttgtt attcgcaatt accatgcttg tgctttgcgt gttagcaagc agaaaagagg
     3121 ttaaacatga acgagttagc aaatcttgac aactatttaa ctgatcctga ttatgctaaa
     3181 ccaccatatg aagcgccaat tgatgaggag gatgaagatg agtaaatcta ccttagaaat
     3241 gagccatcaa gaatggctcg aagaccgcaa aagaggtatt ggtggctcag atgttgcaac
     3301 cgtacttgga ttaaacaaat acaaatctcc ttatcaattg tggcttgaga aaacgggtca
     3361 aattgaattg aaagatttag aaagcgaacc agcttattgg ggtaatgttt tagaagaagt
     3421 ggttgctaaa gagtttcagg aacgtacagg caaaaaagta cgcagaagaa accaagtatt
     3481 tgaacatcca ttacatccat ttttaagagc aaatattgat cgggaagtag tgggagaaaa
     3541 tgccattctg gaatgtaaaa cagccaatca atttctcgga aaagagtggg aaggtgaaga
     3601 ggtaccgctt agctatctct gtcaagttca gcattacatg aacgttttaa acaaagacta
     3661 ttgttatatc gctgttttga tcggtggaca aaaattcatt tggaagcgga ttgaacgaga
     3721 tcaagagctg atcgatacaa tcactgaaca attagtagag ttttgggaaa cgaacgttct
     3781 tggaggtatc gagcctgtaa ttgacggaag tggagcgact gctgacttct taaaagaaaa
     3841 atatgcagat gtagaagaaa atcaaacagc tctaccatca cgttttgatg aacttatcga
     3901 gcaaaaaaga gaactcaagc ggacgaaaaa agaaattgaa tcatctattc gtcaagtaga
     3961 caacgagatc atcagtgagc taggtaaacg tgaagccagt atcggtatca cacaaagaaa
     4021 cattatcagc tggaaacttg ttcgtacaag acggatgaac acgaagaaac tagcagagaa
     4081 atatccagat gtcgcaaatg atgaagagat ttataacgtt actgaatcaa gaaggctaac
     4141 cgaaaaggag atcaaataat atggcaacaa atgaatcgtt aaaaaatcaa ttggcagaaa
     4201 agccacagaa acaagttgca ccaggacagt tagggcttaa agctctaatg aatacaccaa
     4261 caatgagaaa gaaatttgaa gaagtacttc atgacaatgc taatgctttt atgtcgaatg
     4321 ttatgactct tgtatctaat gacagttatc ttgcagatag tgaaccgatg tctatcatga
     4381 gtggtgcgtt aactgctgca acattaaatc ttgggctaga taagaattta ggttatgcat
     4441 atttagttcc attcaatagt aaaaacaagc aaacaggaaa atgggaaaag aaagctcaat
     4501 tcatgcttgg ctataaagga tatatccaat tagcccaacg atcaggtaaa tacaaagcat
     4561 taaatgtgat cgaagtttac gaaggagaac taaaaagctg gaaccgactg acagaagagt
     4621 ttgagtttga tccaaatggt agaacgtctg atgaagtcat tggatatgtt ggctatttcg
     4681 agttactgaa tggattcaaa aaaactgtct attggaccaa acaagaaatc gaagctcatc
     4741 ggattgctaa caataaagat cgcgataaga caaagttaag tggtgtgtgg gcatctgatt
     4801 acaatgcaat ggcacgaaaa actgttttga gaaatcttct ttctaaatgg ggaatcttgt
     4861 ccatcgaaat gcaagaagcc accacatcgg atgagagagt ccaaagggtt caagaagacg
     4921 gcagcattat tgctgaaaca gaagttgaag aagatattcc tgaaagaaaa gaagcagagg
     4981 ttatatctga agaaaatgaa gatgtacaaa ctggattatt tgatgcatct aatccgccgt
     5041 taaacaaata atgagggagc tttctccctt gcttttctag aggagaaata cgaatgaaca
     5101 caggatatgt caaattacat cgaaaagtga tgaattcatt cgtttggacc aatccttata
     5161 tgtataaatt atggagcttg tgtttgatga aagcgagtca tgagaatcgc aagatacttt
     5221 ttaatggaaa agagatacaa gtgaacagcg gagaattcgt cacagggcgc gatgctatca
     5281 catctgagat gaacgaaggc gtgaaacgtg aacatcaagt gaacagcggt tctgtatgga
     5341 gatggctaaa acaatttgaa aaaaacggaa tgttgaacat caaatcaact acgaaataca
     5401 gcgttatatc aataaaaaac tggtctttgt accaaagcag tgaacaacaa atgaacatca
     5461 aacgcacaac aagtgaacaa caagtgcaca caaacaagaa tgaaaagaat tataagaatg
     5521 aaaagaataa taaaacatca tcgtcgcaac cacgcaaaaa gcgtgtttac gacaccgact
     5581 cagtttacta cattctcgcg gaggagttat tcaaacagat ttgtcagaat caggaaatca
     5641 aaaagccgaa tctgcaaagt tgggcagata acattcggaa aatgatcgag atcgacaagc
     5701 ggaccgaaaa ccaggtacgg ggaatgattg aatggagtca gcacaatgtg ttttgggcat
     5761 cgaatatttt atctgctaaa aaattgcgag aaaaatacga cacaatggca gcgcaagcaa
     5821 atcgtgatta taaaacaaaa caaactaaaa cgcttgaata cgagaaattt agcacagatg
     5881 agttgcctat ttgagaggag gcgtaggcat ggaaactgtt ggggaaatca tggaaaagct
     5941 gatacagaaa gtgcttgtcc aacgtggcga atgtcctgaa tgcggacagc ctttgtatgg
     6001 atggcgtacg aagaaccctg atggctcaga acgttgtaag ccaacctgta tgcaatgtgg
     6061 gtataaagca ttgcgagttc aggaagactt acaaacagaa cgaatttaca acgagagcct
     6121 gaaagcaaga gcaatcaatt ttttcaaagg tggttctgtt gtgcctaatc aagcgttgtt
     6181 tgattgcaca ttgcagaatt atcaaattgt cgatcaagaa acaagacaag cggttgaagt
     6241 aaccaaacgc tttgttaatt cagtcttgtt aggaaatcca agtcaccttg ttttaactgg
     6301 aaaacaagga acaggtaaaa gtcacctagc gatggcagcg gcttgggaag tcttgaagcg
     6361 atcaaactac gataaaaaaa tcttatttat cgggttacag gaaatgctgg atcaaatcaa
     6421 attttcgtac aacaatcctg aactcagaaa aacgattgag ggatcgttga ttgcagatat
     6481 caaaacagcg gatttagtca tcattgatga tatcggttca gaactaggaa aagatgcatc
     6541 agatagtcga gcgtttggca taaacacgct aaattcgttc ttggatgcac gacagaacct
     6601 agcaacgatt atcacaacga acttgcttgg tgaagaactg aaaaaagctt acggtacgag
     6661 aacaatatca agaatgtttg tcaactctga tggatttacg atggtatttt ctcaaacagc
     6721 agacaagcgc ataaaaccag tcaaaggagc gatcgcatga atttgactaa acaagaaaaa
     6781 agaatctgtc gtcagataat gttgcaaatt ataggtgatc gtgacatgga cgaattatct
     6841 caacaagaga gtcgaatggt tcggaagata agtaagctaa tcggtgcaga tacgatggat
     6901 cgatccaagc cattacccaa actgaatttt gaatcattta cgtttgagaa atatagccat
     6961 ctgagagagg taggctatag tgtaaatgct attcgaaaag cacttggggt aggaagtagt
     7021 gtatgggcaa aatggcggaa agaaaatgta aatgaggtaa atcaaaatga ttaagctaat
     7081 aggcaaagag acgctcaagg tttactatgt agcggaaaca aaggcagaag tcagccgttg
     7141 gctattgaaa atgtttaata agactagagc caatgcttca gtaagtcaga aagcaagtgt
     7201 atcagaaaca ggcatgcctg aagcaatgtg gatttcaagg aggaactgag atggcaatga
     7261 taccgaagtt tagagcgtgg gatagagaaa aagacagaat gatttatcct agcacgacag
     7321 gagtttgttt tgaaatgaat gatgatggta taagtgtgct tgatattgat gtagattatc
     7381 ccaatgacta tggttttccc aaaatagctt caatcctcat gcaatctacc actctgaagg
     7441 ataagaatgg tgtggagata tttgaggggg atatagcgct tatcactcat aaatcaacga
     7501 actacgccga tacttattgg cacagttacg tagaagtttt tagagcaaaa aacggagcct
     7561 acagaattcg cggtgaacac atctacgaaa cagaattata tagtaataga aaacaattga
     7621 cggtagcggg gaatgtttat aattcaccag aacttttgaa gagagatcga tttatgacta
     7681 ctaccgaaaa tccagagtta ttgaaggagg aacagcgatg aataaacagg aattgattga
     7741 taaatatact gcagaaatat ctagattaag accttattgc cctaatagac atttaattag
     7801 cgatcaactt aaatatggtc tttataaaga aatcctagaa gatttaaaac aactagacga
     7861 accgcagaaa ccagcggtgc ctaagtttgt ggcggaatgg atagaatacg ctaaaaagaa
     7921 aggcgatagt ctagctattt cattcaagcc gtggaacctc tacggtgttg agtatagcaa
     7981 gactgataga tggattgaag ataatcaagg aacgtttgct cgtgcttggc taattggcta
     8041 cgaggtcgag aaagagccgt tgtattatgt gaagttgcca gttgtgtatt ttaatcattg
     8101 ggatttagag acgtatctaa tgaaagatga tagaggaaat ataacaattg cagacaacaa
     8161 cgattttgat gatatgaaat ttacggaatc agaaataaaa gcaattgatg aaagatactg
     8221 gccatttgct gtgccagtgg aagaggtggc ggaaggatga gcaatcttaa agttggagat
     8281 attgtttttt acaaaggtga cgaaggcatc atccgagaag ttatgacgga tggaaaagaa
     8341 aatttttata gggtagatgt cagcagaaaa tctatactct acctgtatga agacgaatta
     8401 gatttaggta ctcaaaaact caacgacaac cagaaaatgg tgtttgatta tcttaaatca
     8461 aacgttgaac aaggtggcaa atcagttatg tattcgatat tccttctagg tgattgggac
     8521 tctcgaattg gagcagcaag aaacgttgat atagcttact gtggattaaa tggtaagcag
     8581 gaagcagaag tcctatcagc attcgctcaa tggggattag aacaggagga agcggaatga
     8641 aatacgaaat accactaagt gaagcagacg tccagtcaat tattaacggt cgggaggtta
     8701 ataaaaaact tcctgatggt actgaattag tcatcagaca aagttatttg aaagatatgg
     8761 cagctccagt attaattgat cgttttaacg tgactgattc tgtggtagag aaccatttaa
     8821 aagaatttcg atcaagtata gacgacactt tcagattagg gagttgattg acaatgaaca
     8881 acagacatcg cagaataaca aaactaagaa aacaggaact gaatgtacta aagacaaagt
     8941 ttgaaaaaga atatggaatt tcagcagaag aaacatataa agtggcaagt cagtgtgttg
     9001 ctgatgcgag taatgctatt cgtaagtttg ggatttcgat attaaatgat gatcgtaaat
     9061 gggaggaaat gagatgaaac taaaagacgg attttacgct agtagtcacg gcatcggcgg
     9121 tttaatgcta gatatgccga caaagaatcc taaaacacgt aagaaaccaa aagtcaaagt
     9181 cggtgacatg gttcgctgcg aagcagaagg gttcatctat ccatttcgtg gatatgtaga
     9241 acacgtctat aatcactcag caatcattcg tattgaaaac acgatggaat gtgataaatg
     9301 gacagctaaa agcaaagaga atttagcagt ggtgagattg gtggatattg aactaatcaa
     9361 tgacaaataa aaaagccgga tcgctccgac tgattcaata aattccacaa gtttattata
     9421 tcacataaaa ggggcggttt gacttgatgc aattgttacg agaggtagct ttcaaacaga
     9481 caagatgtaa tgcgagagat gtgctgaaga actttcggcg tttggagcgg atggcaggtc
     9541 gctctttgat agatattaag tcgccgatta ttacggacat gccgaaggca ccgaagcacg
     9601 gcaataaggc agaagacgcg atcattcaga tgatggatat agaagcagag agagacgcga
     9661 ttttagcggc tttgatggcg cttagtctga ttagtcgtca gatactctac tacagctttt
     9721 gtgtgccaga tagcttctca aactacagga ttagccgtga agtgggttat tcagaaagaa
     9781 gcatacaacg gatgaagtcg gaagctctga tagagtttgc agaagcatat aaacatggga
     9841 aaataattgc ttataaatag gaggactata taatgtggaa agattatgta tcattgaaag
     9901 agttgaaaaa agatcttatt ttcaaaaaaa tcgtagaatg gtcagagagt gaattaattt
     9961 tagaagatgg aaccaaaatg gaagttgtat gtagcgaatc agattgctgc gcatgggccg
    10021 aaggtgaatt caaaaatgta aagcttgatg cagtgattac agatataaag atttttgata
    10081 aaggtaaccg tctctataat ggcgatggac atagttctta tgccgaagtc gttgtttatc
    10141 ataatcgaaa tgagattgct aaagcggaat gtacagcaaa cgatgggaat ggaggctact
    10201 attacagtgt ctgtgcttta aaagtcaaag aaaaactttg tgtagtgact gatgcataaa
    10261 aaatggcggt tttttggcgg aatgatggcg gtttttagct atttaccagt gatattatgg
    10321 tagtgtcgaa agattagtga taggtctgag acaaaataaa atgtaaggga ggaaatctcc
    10381 ctcatcgttt aattaagctt cgatagacag cagcggaaat attaagaata aggatgtgaa
    10441 ttttaactcc ttctaaattg ttcttattat ctatcatccg ttgctgtcta ttgttattat
    10501 gtcactgtgg cggaaagggt agacgcttaa aaataaggtc aatacgtcga gggatagcct
    10561 taacgtttta tgatttgacc atgcaaggtt cgattccttg ccagcgactt agacgaaagg
    10621 tagcttaaat attaaccaca tcaacatcgt cagcgagttg ataaaggtaa ctagcagact
    10681 ctaggattta agccagtctt accgattagt ctgttatagt agcaagcttg ctagaggtag
    10741 ctcctctggt tgacgtgcag ctcagttggt agagcgcctg acttttaatc aggaggtcgc
    10801 cggttcgaat ccggtcacgt taataaagaa cctacggaaa caatcaatct tatcggatgc
    10861 cgattggttg gctgaattaa gcagtgtgtc tgtgaatcag ttaataggca taaaatacta
    10921 gcacagacgt gtgccactct caggtgtagg ttaggagaga aacattagca accgagggtt
    10981 ggaaatgggc gctcaaagta cacgagcaag gcgaggtcga tagtaatcga tggaatcggt
    11041 gtaggttgct attacataat tgattgggtt agattgagtt ttgggattcg gtacaaatga
    11101 atcgtcaaat gactcaagca caggatcgga aacgtccctg cctgtgcatt acatattaga
    11161 tcactcgttg agtggtcttt ttattttaga aaagagagga ttttgaaaat gaatcatgag
    11221 aagtttatcg aaaaatgcaa ggctattgta cgtgaaagaa ttgagaatga gattgctgac
    11281 ctaagtggag cagtacctga gtttagtgtt ttcatagtct ggtcatgcaa aacactacag
    11341 aatagcaaag cattagtcag cgctagctta aaaggagcac cgtattttga aattacgttg
    11401 aacggggaca aaggtgaaat ctatgtagat acttatctca aaaaatcaaa tgaatgtatc
    11461 aaagtctagc aggtgctagg ctttttcttt acataaagga ggaatgatct catgaaacaa
    11521 tataccgcta aagatttcga ggaaatgaag cgattaaaga aggactatga agaagttgat
    11581 atggagctaa ctgttggagt cattcaacga agactgcggg tcggattaga gacagcaaag
    11641 gctatttaca atgatctaaa tgctattgaa gagaagaatg gctaatgagg aactactggt
    11701 atatatcgct aactaatgaa tatcctcgaa ccattgatga ttgttcagtg cgtgttgtgc
    11761 gttctgtaca aatcaaaggg aagtactcta ttgtcgaaat ggggagagaa gcagaaccat
    11821 gtgagattga tgcgtgcaag ctcgtttatt gcggtcatgg tttctatgat gaaccaaaca
    11881 ttcaaaataa tattaacaag aatttgaggg attagaatgc aagaaatggc ttaaaaatct
    11941 ccaatcagga caaatcaagt tagacaaagt ttccgattta aagatattaa ttgaagcaga
    12001 tctaatgttg aaagatattg aaaattagaa aacaaaactc aacctaagaa gattgcgagg
    12061 tggtgtgtat tgaatggcaa gacaacgtga tccaagacgt gatgaagcca aaagaatttg
    12121 gttagaatcc aacggagaaa agcagttaaa ggaaattgca tctgaattaa atgtttcaga
    12181 ttctcaggtt agaaaatgga aatcgcaaga caaatggagc gctgaattga aaagtaacgt
    12241 taccaatggc aaaagtaacg ttactaatca aggtggcgct cctattggta atcaaaatgc
    12301 taaaggtaac aaaggaaata gccgagcttc tccgccagtg ggtaataaaa acgctttgaa
    12361 aacaggcgag tatgaaacca tattttttga gacactaagt gatgaagaga aggacatcta
    12421 ttctagtctg aatgataatc cttcttttgt tttgtctgaa gaaatacgtc tacttaagat
    12481 aagacaattt cgtatgatga agagaatcca acaagctgaa gctggactaa atgatgaaga
    12541 agtcgaacga ttgcagcagc taagaaagat taaaaatccg attgaaaaaa atggtaaaaa
    12601 gctagaaatc aagcgtgagg ttatgcaaga tgtgcagatt agcagaaaaa aacatcgcaa
    12661 aattgatgat attctttcaa ttgaagattc attgactcgg attagcaacc agttagctaa
    12721 agccatcaag caaatgaatg aactttatat gaatgaatac agaactgatt taattaaagc
    12781 tcagactgat aagatccaag ctgagacaaa tgaaattggc ggaaataatt caggtgaaga
    12841 aatagaagaa tggaaacagg cagttttaaa tgccgcaaac aaacgggcgg tgaaagaaaa
    12901 tgaataatga atttattcct tttgctgata ttggttctgc cattgattat tactatgata
    12961 aaccagtagc tttttgccaa gatattttgc atttgaatcc tgatgaatgg caagaaaatg
    13021 ttttaaatga tttagctgaa ttttcaaagg tttctgttcg ttctggtcaa ggagttggaa
    13081 aaacagcatt agaagcagga gcaatacttt ggttcttaac gtgtcgaccc tacgctaaag
    13141 taatagcaac agctccgaca atgaagcaac tttacgatgt actttgggca gaggtagcta
    13201 aatggttaaa tgatagcttg atcaaaaact tactgaagtg gacaaagacc aaaatttata
    13261 tggttggtga ttcagagcgt tggtttgcta cggctagaac agcgactaaa ccagaaaata
    13321 tgcaaggttt tcacgaggac catatgttga ttgtggtaga tgaagcttct ggtgtgtctg
    13381 atccaattat ggaagctatt cttggtacgc tatcaggttt tgataataag ctgttgatgt
    13441 gtggaaaccc caataatatt gaaggtgttt tttacgattc ccacaattca gaccgtgata
    13501 aatacagagt tcataaagta tcaagctatg atagtaaacg tacaaacaaa gacaatatag
    13561 aaatgattct taaaaaatat ggaaaagaaa gtgatgttgc tcgtgtccgt atttttggag
    13621 aatttcccaa aggtgcgttg gattcattta tcagtcttga aacggttgaa ttggctacag
    13681 aaaaacaaat tagtgattct ttagtcaata aaacaacggt tgctcatatt ggtgttgacg
    13741 tagctcgata tggtgatgat tctacgattc tctttcctag aattgctacc agggcattgg
    13801 agtatgagaa gtattcaaaa cgtagcacca tggaaacaac aggatatgtc atcaacatgg
    13861 ccaagaatct aatgagtcaa tatccgagta ttgataaagt gatgattaaa gtcgatgaca
    13921 ctggtgtcgg aggtggtgta accgaccgcc tagaagaact tatagaagac aaacattatc
    13981 cttttgaggt gtttggagtg aataacggtt caacatcaga agacgatttt tacgataatt
    14041 taggtactca actatgggga aacatcaagg aaatgttaga agaaaatatg acagcaaatc
    14101 ttaacggaga acagcctgtt attgaattgc cttctgatag ttcgttaatc aaagaattaa
    14161 gtactcgcaa attcaaaatg acaagtagaa gtcgtatacg tttagaaagt aaagatgata
    14221 tgaaaaagcg aaatattggt agtcccgata ttgctgacgc actggcttta gcgttttatg
    14281 agccaccaag tcactatcaa tttattcaat tttaggaggt gagcttttga ttagtacagt
    14341 attatcttta gagagatata agaagctacg tgttaaatat gcaacacaaa ttgaagatgg
    14401 aatgtttgat ccgaatggtt ttatagaaga tatgaaacca ttttttgctg atcgcgaaag
    14461 aaaatatcta gcttatacta gcgaaaagaa tgaaatagat aatagaccaa agcctaacac
    14521 tgatattgta aaagttaata ataaacttca tgctggtatg tattcaattg tcgtagacca
    14581 agcagtcaat cattttactg gtatacctat caaatgggat tacgatgtat cggaacaaaa
    14641 aagaacactc attcaaagat taaaagataa attcttaaaa aatgatatag aacttccgac
    14701 agttccagaa gcttttaaca aattaacaag caatcttgat tccatgagat tcgcaatgct
    14761 tgattctgaa actgcaactt ttcaaggagc ttgtggagta gctttccgat tgttagagcc
    14821 tgtggaagaa gatgatggtt ggaagttaag agcaagtaat attgaacctt ggagagcaga
    14881 aagatatgga aatgccggaa tctatatcaa agaaaagtat gactcttacc agaaaaaatt
    14941 ttttcaagaa atgaaagtta tgacgagaaa taaaattctg acatatgctt gctatggtga
    15001 tttgaatttt gttacaagcg gaacgttcaa aaaaattgat gagaccgata accctttggg
    15061 gacgattacc ttggcagaat ttaaaaataa tacgaatcgt tattgtgatt ttgaagtagc
    15121 tgaggaaatt ggagatgcaa ttgatcgtgc tttgtctgat caacaaaacg aaatcgaaca
    15181 atttaagctt gcgtacatgc ttgttactgg cacaacaatg agcaaaggca ctgctaaaga
    15241 aatgatgaat cagctaggga ttatcaactt aaaagaccct actgcaaaag ctgaatatgt
    15301 cacaaaaaat ttagcaaaag atttcaatga atatcatatg gatctattga aaaaacagtt
    15361 ttacaccatt tgtaaagcaa tcgattttaa cgatgaggtg tttaaatcaa acagttctgg
    15421 agaagctcgc aagtggcaaa tcattagcct agaagcgaaa acaaatacta aagagcaata
    15481 ttttagagaa ggattgaaag aatgcgcaga aacaattgct gcttttctta agtttcatga
    15541 caaagtagaa attgagcctg aaaaaattat ttttactttc tctagatctt tgccaactga
    15601 tttaagttat ttagctgaag ctttacctaa acttgcacca tacgtatcca aacgaactat
    15661 tcagagccaa attccatttg taacagatgt tgattatgaa aatgaaatga tggaattgga
    15721 aagtggaagt gcttatccag atagtgaata caattttggc ggaggtggca atagtgacga
    15781 tagaaacgaa gtattggacc aaacgtcgag aactggagga tcaagcaagg ctcaaacaag
    15841 aaaatcagac acttaaaaaa ttaactagtg tatttcctga agcactgaaa gagatacaag
    15901 caaaactatt atcacaagct gacttacaca atatcactta tccagaaatg atggagtttt
    15961 atagtacaag taatcagaaa aaatatcgtg aatatgtgga aaaaaattat aagtcattaa
    16021 aaatgtatga tgcaaaatac aaagagttta tcgatgaatt ttttccacca tttgactatg
    16081 caaaagtcaa tcgcttatta caaatacgat cagatgtatt taaaattctt gcagaatatg
    16141 cgatggatgc agatgtgaat caatattttt ctgatcgctt agaggaaatt cttcaaagaa
    16201 catattcttc taatgctaat gtttttgttc aacttttaaa cgttgatata ccaaattatt
    16261 taccagaaaa tgaaattaaa cattatttga actatccatg gtcaggaaag acattttcaa
    16321 gaagactttg gggaaatatt tcatcgcttg aacagaaact ctctaatgct attgtaaaaa
    16381 gtgttgctag tggagaaggc gttatacacg cattaaacac catgagatta gactcagaaa
    16441 tttgtgacat gtttaagtta gaagaatcaa agtataacaa agcgatagaa aatctcgttc
    16501 gaacggagta tgcaaaattt gcacaagatg gtattgaaaa atcatattta gaaacaggta
    16561 ttgaggaata caacgtattg actgcaaaag atgagagagt ttgccggatt tgtggaggaa
    16621 aggcaagtaa gaatccctat aaactgaaag atgctgtcat aggtgaaaat cgagcacctt
    16681 tccatagtcg ttgtagatgt acggatgttc ctaatttacc aaaattagga aaggatattg
    16741 atgaagaata tgaccgttta tttggcgatt tattagatga gtttgcacat gattcttttg
    16801 gaattaattt gaaacggagg aagtagaatg aaagattttt ttgaagcagt actaacaatt
    16861 aatgtaaatg ctgatattgc agaagcctac aaaacagcta ttgagtctga gaaccatcct
    16921 aatggcttga gagaccattg gaatggcaat tatgcctacg tggttattgg cgatcaaact
    16981 gttaattatc aagataatac tccagttgat aagaataccg ttaatttaac gattcaatta
    17041 ttatctcatt cattaccaaa tttaaaagaa acagttgatt ggtatgaaaa gatgggatgt
    17101 attgttgtta gaactgacta caaagaagga aagtctagta attaggcttt tttattttgt
    17161 ccgaaatgac gttaaactag cgcaatactg ggcttggttg aatggtgggg cgcaactatt
    17221 aaaactcaaa gcaatgcggg gcgtgaaaac gaatcgtggg gcgaaaggag aatgagcatg
    17281 aaacacaaat cattaatgcc aatgaatttg caatactttg ccgaaggtga cgatcaaaag
    17341 ttttcttttg atgacttcaa atcttttgtg gaatccaatg aagaagctca aaaatttatt
    17401 cagtcacaat ctcaaagtgt tgccgacaaa caattggaag cttggaaaca aaataactta
    17461 gataaaatta aacaggatac catcaaggaa tatgaggaat ctaagaaaaa caagtcacct
    17521 gagcaaattc aactggaaaa actacaagct gaatttgaag cagaaaaagc gttgcgtgtg
    17581 acaagtgata ataaagcatt tgttgcagaa caaattgctg gattagaact agatggagag
    17641 ttaaaagagt ctatttctca atttatgcta aataatcttg ttagttcgga tacagatttc
    17701 actaagaatg ctgttgaagg ttttacaagt gtcttgaatg caattaatga gaaacatgca
    17761 gatgcattaa aagaactaca aatgaagtct gcatttggtg gaactcaaca atcgaataac
    17821 caggttcagc agaacaatga aacattaaca aatccagaag aacaattagg acaaattctt
    17881 caacaattta actaggagag tgaaaaatta tgaaaaaaac atctttaaat aatttagagt
    17941 atttggatat ttcaccagcg attaatgcta tgcaagtacc aaatacacct tttttaagct
    18001 atttatttgg tgctggaaaa acagagccag caaactcgac agaaattaaa tggcgtgaat
    18061 atgatatcaa caacgatgat tcttctgaaa aacttgaggg cggagaatat ccagatgctg
    18121 aatcaggtcg aacttggttt aacaactata ctgaaatttt tagaaaatca acctctgtat
    18181 ctggtacatt agatgctatt aatgtgaatg gagtcggaaa tgaattaact aatcaagtag
    18241 cactacgtgg tatggaaatg aaaattgatt tgaaccgaaa attgattact ggtgtaaaag
    18301 ctgatgaaaa tggttctaaa ggtcgtcgaa tgaatgggat tttgaacttg atcaattcag
    18361 caaataaggc agaaacagcc actgcgggtg cagtaacaag aaaagatatc gatactttat
    18421 ttaaattgat gtatgaaaaa ggttatatgg gagaaaaact atgcttgatt tctccagata
    18481 tgcaggagtt aatgactgat gagttagatg gaaaatcaac aaaaattgtt cagttcgggg
    18541 aaagagtaac ttttggattg caaattggaa atatcgtgtc taattacggt acaggtattg
    18601 ctctactaga accatcattg ccaaaaggaa caattgccgc aatcgatact aattatgtga
    18661 aactacgtcc attacgtgaa tggagagcag aagaacttgc aaaaacaacg gattctagac
    18721 gtatcggtct tgtaggtgaa tactctcttg aatacaatgc ttcaaactct ggggcaattt
    18781 taaatttaaa gtctgaataa aagggagtta gtactccctt ttttgatagg aggaattaca
    18841 gtggtaaaaa aagatgaaac taaaaaagat gaagtcgtga aatatagagt aggtaaaact
    18901 aaaaattttg ttgggtttgt tcatcctaaa actcgtagat ttatcacagc agattcaaat
    18961 aacgaattta tcatttctat agatgataaa gaagcaattg caattttgga agatgcaatt
    19021 gatgttaatg aaatttagga agtgatctga tggatgaatc gctaaaaacg gaaatcattg
    19081 agtctacaaa agaagatttt ccagatttga gtgaagaacg cataactaat ttattagaaa
    19141 taattttgct agaaattgaa tcatacaaca cttgtaaaaa tgatatttca tgggaaaagt
    19201 taaaaagcgt gattagtgaa gtgttgtata aaataataaa aaatgaatca gaaaaaacag
    19261 tatcttcagt tagacgtggc gatacaacga ttagttatgc ttcaacaaca aatgatgtta
    19321 gtgagttact tctaggctat ggtgatttga tacgaagagt tattggttgt ggaggattgg
    19381 agttttttta atgaatgaag cagatatttt agaaactact tatgaagata gttgtattat
    19441 tgaaagactg acggacattg aagattctaa tacaaatatt actattcaag attacaaaaa
    19501 agtatatgat aatcctattt cttgcgctct ttcacaaggt caaatcgatg gactagcagt
    19561 catagaagat ggagagatgg taaatgtttc aactgacaca tataaattat ttgttcatcc
    19621 taagattaaa ctaaaaaaag gagatcgaat aacaataact caaaaggcca gtggcttaat
    19681 tttttctcta tttgctacta agccttttta ctatcctagt cattgtgaag tgaatttgat
    19741 aggaagtgag aaaaatgggt aatctcaaat atgaatcaaa tgcagaaaag atcattgaga
    19801 attttaaaaa tatgactgta attgctcaaa aagaaggaat atccttcgtt aacgattcaa
    19861 tgaataaagt cgttagttta gctaaaccat taactcctgt aaaatcaggt aatttaaggc
    19921 gaggatatcg tgtagtaaaa gctagaaaac tatcaagcgg tcgaattgtt ggagcagtta
    19981 taaacaatga acattatttt aaatatgtag aagaaggtca taggactaaa aatggtggct
    20041 ttgtcaaagg gaaattcatg ttaactcgtg caacgaatct tgcaaatatg tcttatattc
    20101 ctcgaagatt taaacaaatg gcaataaaaa tcgttaagaa aggaaagtaa catgaaagat
    20161 gaaatcattg ctgcaatcag tagcaaatta aaagaaatct atcctgatgg gacaatatat
    20221 cttgattcag ttatgcagtc aactaaagat ttttatttcg ttttatcagt aatggaatct
    20281 ggaactgaaa atgtaggaat tgatattcaa aatgtttctt tcttaattga tattgcattg
    20341 attgataata aacctaatag aaatttaatt aatgaattag tctcacgttg tgggactttt
    20401 tttaatacga ttacaatcga tgagcaaata ttatttccaa aagcctattt acctgatgaa
    20461 gcagatggtg ttcaacatat tcgttttaca ttagaatttc cacaatatat tgaatggagt
    20521 gaaagataaa tgggagaaaa aagaagtaaa accggaatta tttctgtcga aaaacctact
    20581 tggtttcctt tggaagatga gacagggaat tttccggtat atggtactgc gttgccaatg
    20641 ggaacagcag taagtatcaa accaacagct aattatgaaa caacacaaga ttatggggat
    20701 tcagtggttc aagatcaatt tacagctttt ggtggtgcag aagttgaact ggaagcaaat
    20761 ggatatactc ataaagtttt atctacaatt actggaggga aaattgttaa aggtggtgcc
    20821 ttgcgttctg gagaagatat tgcgcaagat ggtgcgtttg catatagacg taaaaaatcg
    20881 aatgggaaat accgttatac ggtcttttat aaaggacagt ttgctctaga ttcagatgaa
    20941 acatcaacga ttgaaggaag taaagtaagt tttactcatc caacttggaa gggttcattt
    21001 gtagatgtac caggacttgg atatatgtat tcagttgatg aagatgatga aggcgtggat
    21061 aaagcaatga tcggaaattg gtttacaaaa gtagcaattc caattgaaga agcagaactt
    21121 tcaggaggta cagaataatg tcaaaatatc aaacaacgat taaattaacg aaaaaagatg
    21181 aagaaggtaa atatgagcaa gtacaattta aatctgctga atttttacca ggaacagttg
    21241 tagaagatgc tgcaggagtt atggaagaaa tgcaaacagc gactgataaa caatcagtta
    21301 aaaaagcttt aagtcgtgct tattcattta ttgcagatac tttatttgag ggacaattta
    21361 ctggtgaaga ttattgcaaa ggaattgatg ctcgtgagat tgcctcattg acaggaaaat
    21421 tattgaagtc tgttactgca ggttttgatg aaacttatac agagacgaaa aaaaagtaag
    21481 tgaggcgctc aagtcacctt catttaagta ttcgattact taccgagaat tagatataaa
    21541 gacgcaatta ctcgaagcag gttggacgtt accagaaatt gaacgtactg acttggatga
    21601 gttaatgcgt ctttttgctt ttagagatgc ggtaaaagag catgaagatg ttgagtacta
    21661 tgataatttc actcaatttt aggaggtgat actttgaata acgatgacct aattctgaag
    21721 atgatactag atgaatctgg ttttactgcc ggtatgaata atgctgtcaa aaagttgaac
    21781 tcttttgatg aaacaatcga aagaaaaagt agaaatagtg gcagttcatt aggtagtatt
    21841 tggaaaatat ttgctggaag ttttctagct agcggagtaa ctagaatcgt aggagctggc
    21901 tttgatctaa tcaagggttc cataagtgga gcggttgatc gagtagatac gatgaataac
    21961 gctctacgaa atttccaaaa tatgggattt agtaattcag aaattatgaa gaatatcgga
    22021 aagaatgggc ttttatctca aggtattcaa ggacttccta ctgctttgaa tgatgcgata
    22081 agtcatgttc aacttcttgc ttcttctaca ggtgatttaa ctcgttcaac tcaaatattt
    22141 aaagctttaa atgatggaat tcttggtttc ggtggttcaa ctgatcaagt taatgaagct
    22201 gttattcaat tatctcaaag tttctcaaac ggaaaagtag acgcacaaac ttggaactca
    22261 atgatcaacg ctcaacttgg acctactctt tctgctattg ctaaaaagat gggaattaca
    22321 atgggagatc tgaaagaagg tttgtctcaa ggtaagattt ctgttgaaga attccaaaat
    22381 caattaatag aaatggatac caaaggtggc ggaggactta aatcattaag tcaaatcgct
    22441 aaagattcta ctaagggaat taaaacctct atacaaaatg caaaaacagc tgttacacgt
    22501 ggtgttggtg aggtaataga aggattaaat aaagcattag tagattcaga tttaggtgga
    22561 tttaagggaa ttattgataa agtgggcagt tcaatggaat cgttcttaaa agtaattgct
    22621 gcaaatattc ctacagcagt atcattttta agtaatttat ttaacgaaat tcaaaaattt
    22681 ggttctgctt tgaaattcat gatgcccttt cttgttcctg cagctaccgc ttttggagcg
    22741 ttcatgtttc aacttaaagg tataccagca ataataaaaa gcttcaataa ttttaagaat
    22801 gccataattg gcgttggaaa ttcattaaag attatggggg caatagctgc tgctaattca
    22861 tttgttttaa ttattggtgc agtcgttggt gcaatagctg ttttcggtta ctttatggca
    22921 accaatgaag agtttagaaa caaggttata tctatttgga atgatgtgaa agattccgta
    22981 attggtgtat taaaaaatat aaaggactgg ggaattgata cttggaattc tgctaaagaa
    23041 atggcatcaa atgcagttga gggtgtcaaa gatgcttggt caggaacaaa ggaatggttt
    23101 tctaacacct ggaaaggtat taaggatggg gccacaggtt tatttgataa aacagtagag
    23161 acttcaagaa atgcagtcga tagtgtaaaa aatgcatggt ctaatatgaa gaaatggttt
    23221 tctgatactt ggcaaagtat aaaagattca gccagagaaa aatggaatga aattaaaggt
    23281 tctattatgt cggttgctgg tccattgatt acaggtataa agaatgcatt tttacatgtt
    23341 actttttatc tagagacctt atggaataat ttagttgaga ttggtaaaaa cgtctttgag
    23401 attttaaaaa atgttatact tgcgccggtt ttgtttatta cctccatgat ttctggtgga
    23461 tgggaagaaa caaaaaataa tatgattgct gtttggaata atattaaaga aagtgccata
    23521 aatatttggg aatctataaa gaatatattt gtaagttatt ttacaaatat ttattttgct
    23581 gcactcaata tttggacagg gttcaagctt acattgataa atatttggaa tgaagtggta
    23641 aatcaagcta aatcgatttg gttaaacata aagtattttt ttattaatct ttggattgat
    23701 ataaaatatt ttgcaat
//
LOCUS       DACDUU010000043        23673 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.043, whole genome shotgun sequence.
ACCESSION   DACDUU010000043 DACDUU010000000
VERSION     DACDUU010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.043"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(45..779)
                     /locus_tag="IWL30_001708"
     CDS             complement(45..779)
                     /locus_tag="IWL30_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP9706464.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1106..1516
                     /locus_tag="IWL30_001709"
     CDS             1106..1516
                     /locus_tag="IWL30_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAP9706465.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1509..2210
                     /locus_tag="IWL30_001710"
     CDS             1509..2210
                     /locus_tag="IWL30_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAP9706466.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2210..3796
                     /locus_tag="IWL30_001711"
     CDS             2210..3796
                     /locus_tag="IWL30_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9706467.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3815..4036
                     /locus_tag="IWL30_001712"
     CDS             3815..4036
                     /locus_tag="IWL30_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAP9706468.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4033..4794
                     /locus_tag="IWL30_001713"
     CDS             4033..4794
                     /locus_tag="IWL30_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAP9706469.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5174..5683
                     /locus_tag="IWL30_001714"
     CDS             5174..5683
                     /locus_tag="IWL30_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP9706470.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5758..6297)
                     /locus_tag="IWL30_001715"
     CDS             complement(5758..6297)
                     /locus_tag="IWL30_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAP9706471.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6437..7048)
                     /gene="rpsD"
                     /locus_tag="IWL30_001716"
     CDS             complement(6437..7048)
                     /gene="rpsD"
                     /locus_tag="IWL30_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAP9706472.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7364..8161
                     /locus_tag="IWL30_001717"
     CDS             7364..8161
                     /locus_tag="IWL30_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAP9706473.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8189..8824
                     /locus_tag="IWL30_001718"
     CDS             8189..8824
                     /locus_tag="IWL30_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAP9706474.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8844..9617
                     /locus_tag="IWL30_001719"
     CDS             8844..9617
                     /locus_tag="IWL30_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9706475.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10025..10822
                     /locus_tag="IWL30_001720"
     CDS             10025..10822
                     /locus_tag="IWL30_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAP9706476.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10800..11213
                     /locus_tag="IWL30_001721"
     CDS             10800..11213
                     /locus_tag="IWL30_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706477.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11197..13842
                     /gene="mutS"
                     /locus_tag="IWL30_001722"
     CDS             11197..13842
                     /gene="mutS"
                     /locus_tag="IWL30_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP9706478.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13860..15968
                     /gene="mutL"
                     /locus_tag="IWL30_001723"
     CDS             13860..15968
                     /gene="mutL"
                     /locus_tag="IWL30_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAP9706479.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15990..16550
                     /locus_tag="IWL30_001724"
     CDS             15990..16550
                     /locus_tag="IWL30_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAP9706480.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16635..18623)
                     /gene="treP"
                     /locus_tag="IWL30_001725"
     CDS             complement(16635..18623)
                     /gene="treP"
                     /locus_tag="IWL30_001725"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAP9706481.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18846..19559
                     /gene="treR"
                     /locus_tag="IWL30_001726"
     CDS             18846..19559
                     /gene="treR"
                     /locus_tag="IWL30_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAP9706482.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19636..20082
                     /gene="msrB"
                     /locus_tag="IWL30_001727"
     CDS             19636..20082
                     /gene="msrB"
                     /locus_tag="IWL30_001727"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAP9706483.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20252..20854
                     /gene="ruvA"
                     /locus_tag="IWL30_001728"
     CDS             20252..20854
                     /gene="ruvA"
                     /locus_tag="IWL30_001728"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAP9706484.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20867..21868
                     /gene="ruvB"
                     /locus_tag="IWL30_001729"
     CDS             20867..21868
                     /gene="ruvB"
                     /locus_tag="IWL30_001729"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAP9706485.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21897..22475
                     /locus_tag="IWL30_001730"
     CDS             21897..22475
                     /locus_tag="IWL30_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP9706486.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22527..23009
                     /locus_tag="IWL30_001731"
     CDS             22527..23009
                     /locus_tag="IWL30_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAP9706487.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23126..23500
                     /locus_tag="IWL30_001732"
     CDS             23126..23500
                     /locus_tag="IWL30_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706488.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7728 a         4272 c         5078 g         6595 t
ORIGIN      
        1 ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc cataaataat
       61 aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac tggtacccat
      121 gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg tacaagacgt
      181 ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag tgaagcaact
      241 aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc ttggaagaat
      301 ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa gaattcattg
      361 acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc taacacatgg
      421 tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat caattgtcca
      481 atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca agggaataga
      541 ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct gattccacca
      601 aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag catccagtca
      661 ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt acctagaacg
      721 attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga gtaatccata
      781 aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg aaaagcatcg
      841 gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat aaaaaaatga
      901 attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt taaaagttct
      961 ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt tttctcaaaa
     1021 attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta gtaaaatgaa
     1081 agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca ttattatttt
     1141 cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc ctggaagtat
     1201 cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc ctgaaaaaat
     1261 cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc ctgctggtgt
     1321 aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac tgggagcagt
     1381 cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag gtgtagaagc
     1441 tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg aaatggagga
     1501 gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg actttaggag
     1561 tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg aatcctctcg
     1621 tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc tataaagact
     1681 ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg gcacttgcca
     1741 tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta atggctgcga
     1801 ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac ttcttctctc
     1861 ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct atttctgtcg
     1921 atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt gtctctacag
     1981 gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc gaaagtccag
     2041 ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca aaagcaatcg
     2101 agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac ggaatattga
     2161 ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga tgcgaaaacc
     2221 actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga aagacgtgta
     2281 tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga ttctcctcct
     2341 agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag ttgttcagct
     2401 ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc agcagtttag
     2461 cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact tttatgatac
     2521 gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta cgctgaaatt
     2581 ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat ttgcgcaaaa
     2641 gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac agcaactgca
     2701 aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag aagaaatcat
     2761 tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct ggccatttga
     2821 aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt tttttaatgt
     2881 tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct tttttttaag
     2941 aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga tttctgacaa
     3001 tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt attttcaagt
     3061 agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat attatagtga
     3121 tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac cgccctttac
     3181 agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg ggctgccagc
     3241 agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt tcgcctatct
     3301 ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta atcatttagt
     3361 tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta gacacaaaca
     3421 tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc tgactgtgtt
     3481 taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt taatgacaga
     3541 tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt tccgtaatga
     3601 gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc ttctgatttc
     3661 gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg tgactgaaga
     3721 actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc aaaaacaaaa
     3781 actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa ttttaccaga
     3841 attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac tttatgttgt
     3901 tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat tgatcaaaac
     3961 aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag cagatgattg
     4021 ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt atttatcaca
     4081 ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca agatgctttt
     4141 gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg atttcctgaa
     4201 acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca agcggtatcg
     4261 aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg gatattggat
     4321 gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta tgagacgaat
     4381 gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg ccaaaaaagc
     4441 ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg tgggatcgct
     4501 tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct ggattacgca
     4561 ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc aatgaatgcg
     4621 gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac accagtcaag
     4681 cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc tcctcaagca
     4741 agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa ataaccttgt
     4801 gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg atgataaaaa
     4861 aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa ttctttttaa
     4921 taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa acgatattca
     4981 aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag ctatttttat
     5041 ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc gacacttcag
     5101 ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt atcttgcaaa
     5161 ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga caacgacaga
     5221 tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg ttttgtctgt
     5281 ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc tgtccttgta
     5341 taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata ttgcttctcc
     5401 tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat tgcttatcaa
     5461 ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga cagcatgcgt
     5521 atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc aactaccttt
     5581 tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg tcggtgggat
     5641 aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg tttaatttaa
     5701 aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct tttttgatta
     5761 atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat aagctgctcc
     5821 agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa atgcagttgt
     5881 aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg cagcaagaga
     5941 gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt cgatcatcca
     6001 accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg gcccaaataa
     6061 aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc caagtaaaag
     6121 ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg ttaatccaat
     6181 agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct gagaaaggac
     6241 agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc ggttcataac
     6301 gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa acaaagtgat
     6361 tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc ggtctttttc
     6421 aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga gcttcgtcga
     6481 tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc aatttttcag
     6541 cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct tttactgtta
     6601 cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg tggtatgaag
     6661 ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat tgacgtgctt
     6721 gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt tgttctagta
     6781 agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt acgaacaagt
     6841 tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt tcagttaatt
     6901 gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct ggtgcgtaag
     6961 ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga cgagaaattt
     7021 tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat aagtttttgg
     7081 agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat cttcaccttt
     7141 gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat gagttattat
     7201 ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca cgaatgtcaa
     7261 gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa tgagagggta
     7321 tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc cggtatcacc
     7381 attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg aaaacagctt
     7441 ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag gaacggctgt
     7501 ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa agattttata
     7561 cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg aattaggaac
     7621 atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt taagtaaggc
     7681 agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat tatgtggatt
     7741 tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta tgttagaatc
     7801 aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga tgtttgcgaa
     7861 tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg tcggaaaagt
     7921 gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac acgtgatagc
     7981 gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg atggaatgac
     8041 agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata ttggtggcgg
     8101 tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct atgtggacta
     8161 aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa aaaatgagga
     8221 aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg gagtacctaa
     8281 gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt tatctatttc
     8341 tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt atcagtatgt
     8401 agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact tagtcgtatt
     8461 gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga gaaaagtata
     8521 cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat tgtttgaaaa
     8581 aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt ataaacagca
     8641 aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa aggaattctt
     8701 tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg ccgattgggt
     8761 aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa gtaagtattt
     8821 ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat attgatggaa
     8881 cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg gaaaaattac
     8941 gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat gcgcaagaga
     9001 tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc ggttttttag
     9061 accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg ttcgttcaag
     9121 aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc aagcgttctt
     9181 cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct gtcttgcctg
     9241 aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca tttttcgatc
     9301 aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc tggcatgaga
     9361 acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg cacttagcag
     9421 aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa aacgaccgtg
     9481 aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca gaagtacaag
     9541 cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag gcattgaaag
     9601 agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt aataagccca
     9661 gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc gcagatgcaa
     9721 gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc aagaaataag
     9781 aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat ttctgaagaa
     9841 gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg tcatagtccc
     9901 atttttttat tttatttttc aaaactaaga aataccagga aagagttcct tttgtttgac
     9961 cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag aagaggaatt
    10021 tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag agatgattac
    10081 tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg taaatggtga
    10141 gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt tgcaagacgg
    10201 cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct ttgagtttgt
    10261 aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc ctggacaagg
    10321 gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc aggctcgttc
    10381 ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag aagaagccag
    10441 aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg aaaaacaagc
    10501 aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata cacacgttca
    10561 gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg ttgggatgac
    10621 aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa agtttctgac
    10681 tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa gcggctgtat
    10741 cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc agatcaacga
    10801 tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag ccggaaattc
    10861 aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc attcgctaca
    10921 aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga gaaaaaatca
    10981 aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca gcagaaaaag
    11041 aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg cttgttgtag
    11101 cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca actatgatac
    11161 aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac taaaaatact
    11221 cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt tttgttctat
    11281 cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc acagcttttg
    11341 gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat gtgcggtgta
    11401 cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta taaagtagcg
    11461 atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg cgaagtgatc
    11521 cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa agacaacaat
    11581 tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc cgatttaagt
    11641 acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa tgaagcctct
    11701 gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct tcgtgaacag
    11761 ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga aaacgcggaa
    11821 tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac cggcaaacta
    11881 ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa agcagtagaa
    11941 taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct agaattaagc
    12001 caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga tgaaacaaaa
    12061 acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat ccaggcaaaa
    12121 caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt tgaacgtatc
    12181 gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg tcgagtagct
    12241 ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga acaagtgcca
    12301 atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct gctttcagaa
    12361 atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga tgatccgccg
    12421 ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct cgatatgtac
    12481 cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa agagcgtcaa
    12541 gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta ttatatagaa
    12601 attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa acaaacttta
    12661 gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca aatcctcgaa
    12721 gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag agaagaagtc
    12781 aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt agatgtgcta
    12841 caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact tgtcagtgac
    12901 aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt attaggacat
    12961 caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt gctgattaca
    13021 ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac tgttctgatg
    13081 gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt tgatcggatt
    13141 tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt tatggtagag
    13201 atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat tttgtttgac
    13261 gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc aatcatcgaa
    13321 tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca tgaactgacg
    13381 gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt tgaaaaagac
    13441 ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag ctacggcata
    13501 catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc agatatcctg
    13561 catacgctag aaaatggcga taacggacac catactgttc cagctgaagt agaagagaaa
    13621 actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa tcaagaccat
    13681 atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga attatccgaa
    13741 aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat gacgccaatg
    13801 gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg cggatatcga
    13861 tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg ggagaagtag
    13921 tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac gccggcagta
    13981 ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg attgataacg
    14041 gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg acaagtaaga
    14101 tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg gaagctttgc
    14161 caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa gaaaaacaag
    14221 gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca ccactgcgtc
    14281 aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc ttgaaatacg
    14341 tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga ctggcattga
    14401 gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg gcaactgctg
    14461 gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc gcaaaaaaaa
    14521 tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt tcattgcctg
    14581 aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt tttatcaaga
    14641 acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg gtcggtcgtt
    14701 tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta aatgttcatc
    14761 cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta attagtaacg
    14821 ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat aatctacggt
    14881 ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat ttgtcctacg
    14941 cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga gagacaggta
    15001 cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat aagtctgtgg
    15061 aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca gaaaaaacag
    15121 cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa acagtggaaa
    15181 aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat gaagaacaag
    15241 cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca ctccatcctg
    15301 aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag aaattagaaa
    15361 cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg cacggaacgt
    15421 atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct gcccaagagc
    15481 gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat ctgcaagaat
    15541 tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta aaagagcaaa
    15601 aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac agctttattg
    15661 tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt cgggagatga
    15721 tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa gcaacagcaa
    15781 tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa gcacaagcac
    15841 gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc catggccgtc
    15901 cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga atccaagacc
    15961 cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc caatcaccaa
    16021 gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct gctgatatcg
    16081 atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg gctgaagaaa
    16141 aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt gccagtgaca
    16201 cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct gaggctgaat
    16261 tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt gttttaaaaa
    16321 caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat cctttgacag
    16381 acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca ggggcatacg
    16441 gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat tacagtatcg
    16501 taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa acaaaaaagc
    16561 ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt gaaaagatta
    16621 cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc cgggcgaaca
    16681 agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa agcaacaata
    16741 atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc aatcagggtc
    16801 tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc ctttagttca
    16861 acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag aataccaatg
    16921 gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata gactttgccg
    16981 tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac tgggtcattt
    17041 acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc ttttgttgca
    17101 actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt tgcgccattt
    17161 gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat cttattcaaa
    17221 attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc aatgatcatt
    17281 gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg caaaccgcct
    17341 actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc taatccagag
    17401 ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa catagcaggt
    17461 tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc ttctttttta
    17521 ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat atttgataaa
    17581 gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat atcgatggca
    17641 ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc gaatacggca
    17701 ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga gatccagctt
    17761 ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt tggtaacaat
    17821 gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg gaagaagatt
    17881 tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc ttgataacca
    17941 atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc ggctgtggaa
    18001 gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa aacgattcct
    18061 aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac tggaagaaaa
    18121 tggaaaattg cttcaccggg taaccacaaa aaggcattga caccattcca aaacttcgag
    18181 tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac atttctgaat
    18241 ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat ttctgctaaa
    18301 acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt tccttgttct
    18361 ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt agaaacatcg
    18421 tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac gctaggtata
    18481 tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg catacgagtc
    18541 gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa cagcttctcg
    18601 gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg tctatgcaag
    18661 ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt attaaaaagt
    18721 acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa aaataggaac
    18781 attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg agtgagggaa
    18841 aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt ttagcgaaag
    18901 aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt tatggtgttt
    18961 cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat attcaaaaaa
    19021 aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct atttctggat
    19081 tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg atcatccata
    19141 cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg gagatcggtg
    19201 cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt atattagaca
    19261 ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca caaggatcga
    19321 tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa aaggagatca
    19381 cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat gacacttatg
    19441 cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag tattctgagt
    19501 cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga aaagtgtaaa
    19561 tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga taggatcaca
    19621 aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact gaaagaaaag
    19681 ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc atttagcggg
    19741 aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg tgaaccgtta
    19801 ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag caaaccaata
    19861 acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg aatagaagtt
    19921 cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc agtagatcaa
    19981 ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata tgaaaagatg
    20041 gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata cacaaagagc
    20101 ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg attgctattt
    20161 gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca ataaagggaa
    20221 cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag gtgtggttac
    20281 ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc agatcgcact
    20341 aggaaatcct taccgctaca gtagcaaatt aaatcaaaaa ataaaaattt atgtccatca
    20401 ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag aaaagcaact
    20461 gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg caatcatggc
    20521 aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca cttatttgac
    20581 gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc taaaaggaaa
    20641 attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa acacaaaaga
    20701 cgctacagct ctatctgaag caatggaagc tctctcggct cttggttaca gtgatcgaga
    20761 aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg atgagtatct
    20821 acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga cagatgaata
    20881 cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc ttcgaccaca
    20941 gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga tttacatcga
    21001 agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc ctcctggatt
    21061 aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata ttcgtacgac
    21121 gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg aactggaagc
    21181 aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag aagagatgct
    21241 ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc caaccgcaca
    21301 tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac gggcaggtat
    21361 gctttctgca cctttacgag atcgatttgg catcatttct catatggaat attatgacga
    21421 acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag agatcattga
    21481 agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg ctaatcgctt
    21541 gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg atcgtcctat
    21601 agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt atgttgacca
    21661 gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt taagtacgct
    21721 ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac cgtatctgat
    21781 ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt atgcctatga
    21841 acattttggt tacgactatc atatatgatt tttgctttta gattggagga aaatgaatga
    21901 gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg tgccaaacgc
    21961 aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc aatcgacaaa
    22021 cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat tgtgaggatg
    22081 cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag caagcattga
    22141 aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg ctttctgacc
    22201 cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc aagatttttg
    22261 aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat gctcgttttt
    22321 tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat gaagaaacac
    22381 cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta tattcttatc
    22441 gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg ttggaaaagc
    22501 aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt ttgggaaata
    22561 tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta gaaggaaggg
    22621 aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga aagacaccgc
    22681 accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat atggttgctc
    22741 ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg gatcaagaaa
    22801 atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc catttgtttt
    22861 taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat accaataact
    22921 ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat atttggattg
    22981 atgaaaataa aataacaaac aatttgtaaa gttaacgctt tctttttaag gcgatgttag
    23041 agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa gtaacacttt
    23101 cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa tgaagaagct
    23161 tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa atgacactga
    23221 agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc aaaaagctgc
    23281 tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta gcaagtatga
    23341 aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga tgatccgcct
    23401 acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa aaataacaat
    23461 cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag atagaaaagc
    23521 acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc tttaagctga
    23581 tatcagtaaa acggaacgtt ttaggatgag tatcacagca tgacagcatt gggttgatta
    23641 gttgaatcga ttggatggta caagtttttt tga
//
LOCUS       DACDUU010000044        23529 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.044, whole genome shotgun sequence.
ACCESSION   DACDUU010000044 DACDUU010000000
VERSION     DACDUU010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.044"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            6..305
                     /locus_tag="IWL30_001733"
     CDS             6..305
                     /locus_tag="IWL30_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9706489.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IWL30_001734"
     CDS             378..1739
                     /locus_tag="IWL30_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP9706490.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IWL30_001735"
     CDS             1761..2021
                     /locus_tag="IWL30_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706491.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IWL30_001736"
     CDS             2035..2787
                     /locus_tag="IWL30_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP9706492.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IWL30_001737"
     CDS             complement(2881..3486)
                     /locus_tag="IWL30_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9706493.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IWL30_001738"
     CDS             complement(3539..4513)
                     /locus_tag="IWL30_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAP9706494.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IWL30_001739"
     CDS             complement(4891..5670)
                     /locus_tag="IWL30_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9706495.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IWL30_001740"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IWL30_001740"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP9706496.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IWL30_001741"
     CDS             7161..8558
                     /locus_tag="IWL30_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9706497.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IWL30_001742"
     CDS             8639..9079
                     /locus_tag="IWL30_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9706498.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IWL30_001743"
     CDS             9227..9553
                     /locus_tag="IWL30_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9706499.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IWL30_001744"
     CDS             9554..10303
                     /locus_tag="IWL30_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706500.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IWL30_001745"
     CDS             complement(10383..10838)
                     /locus_tag="IWL30_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP9706501.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="IWL30_001746"
     CDS             complement(11265..11540)
                     /locus_tag="IWL30_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706502.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="IWL30_001747"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="IWL30_001747"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAP9706503.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="IWL30_001748"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="IWL30_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP9706504.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="IWL30_001749"
     CDS             14770..17436
                     /locus_tag="IWL30_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9706505.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="IWL30_001750"
     CDS             17800..18552
                     /locus_tag="IWL30_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9706506.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18574..19728)
                     /locus_tag="IWL30_001751"
     CDS             complement(18574..19728)
                     /locus_tag="IWL30_001751"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAP9706507.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            19913..22354
                     /locus_tag="IWL30_001752"
     CDS             19913..22354
                     /locus_tag="IWL30_001752"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAP9706508.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(22684..23388)
                     /locus_tag="IWL30_001753"
     CDS             complement(22684..23388)
                     /locus_tag="IWL30_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706509.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         7520 a         4127 c         4871 g         7011 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatgatt ttattatatt ttgtagtaac gaatatcatt
    18601 ttcgatcttt gtaatcgaaa aaggtacatc tgaagtatat tctgcacatt ctccgtgttc
    18661 aggaagcgtc tcactgaaaa tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc
    18721 tagcatctct tgatgagatt gtgcgaagag atattcttga tagttttctt ccagataacc
    18781 tgtaaaaaat tcacttactg caccagaacc gtagctgaat agaccgatgc gatctcctgg
    18841 ctgtagtgat ttcgaatttt ccagtagaga agttagcccc aggtataatg aaccagtgta
    18901 aagattaccg attcgtcggc tgtaacggat gctttcttca tagcgagcca taagacgttc
    18961 ctgattttct tcgtctgttt ggtctaatac gctttgcaat gccttttttc ccatcttagt
    19021 atacggaata tggaaagcaa tcgcctgata atcttcgagt cctcgacccg acaattcttt
    19081 atgtcgattc caaacttttt ggaatgattc gatatacgta gaattagata aaggaccatc
    19141 aacaacagga aattcgctat aatctggacg ccagaaatca tagatatctt ctgtcagaaa
    19201 tacgctgtcg tcttcaatcg ataaaatacg cgggttttga gtgatcatca tcgcaacagc
    19261 accgacacct tgcgtcactt caccaccgct tgccaagccg taacgagcaa tatcacttgc
    19321 tatgactagt acttttcgtt ctggatgatt tttgacatat tctttcgcca tgtgcaaagc
    19381 tgcggtgcca ccgtagcaag cctcttttac ttcaaaagaa cgagcaaaag attggatttt
    19441 cagtaaatgg tggataatta ccgcactcgc ttttgaatga tcaatcccgg attctgttcc
    19501 gacgatgacc atgtcgatta gctcacggtc tttttctgtt acgattttgc tggcagcatt
    19561 tgctcctagt gtaatgatgt cttcatttgc acgattgact gccatttggt cttgaccaat
    19621 ccctatatgg tattttgcag gatcatcccc acggctttct gccaactctg tcatatctaa
    19681 atataaatta ggaataaaaa aggaaagacg atcaatccct attttcattt caacacctct
    19741 ttacttatga taaaaaactt actttactaa ggtatcataa aaagattaaa agaaatataa
    19801 aaaagagaat atttaaggat tgttaaaatt taatcttaga aaaaaataac tggaacaaga
    19861 gctatttgtt tttacagcaa agaatggtat atttttgtag gaggtggctt acttgaaaga
    19921 agtcgttatg atagatgctg caagaacgcc gataggaaag tatcgcggaa gtttaagtcc
    19981 atttactgca gttgaattag gcacacttgt gactaaagga ttattagata aaacaaaact
    20041 gaaaaaagat aaaatcgatc aagtaatatt tggaaatgtc ctgcaagctg gtaatggtca
    20101 aaatgttgcc agacagattg ctttgaacag cggtctccct gtagatgtcc cagcaatgac
    20161 gatcaatgaa gtttgcggat ctggtatgaa agcagtgatc ttagcacgcc agctgatcca
    20221 attaggtgaa gcagaacttg tgatagccgg cggaacagag agtatgtctc aagcacctat
    20281 gctgaaacag tatcagtcag aaacaaatga atatggtgaa ccaatttcca gtatggtcaa
    20341 cgacggattg actgacgcat tttcaaatgc acatatggga ttaaccgcag agaatgttgc
    20401 aacacaattt tctgtgagca gagaagagca ggatcgctat gccttgtcgt cccagttgaa
    20461 agcagcacat gctgtcgaag ccggtgtatt ttctgaggag atcatcccag tcaagatttc
    20521 tgatgaagac gtgttatctg aggatgaagc agttcgtgga aatagtacat tggaaaaact
    20581 gggcacgtta cgtacagtat tctcagaaga aggaactgta acagcaggaa atgcttcccc
    20641 gttgaatgac ggtgcctctg tggtgatcct tgcatccaaa gaatacgcag aaaataataa
    20701 tctgccttat ttagcaacca tcaaagaagt agcggaggtc ggtattgacc caagtatcat
    20761 gggaatcgct ccaatcaaag ccattcaaaa attgacagat agatcaggaa tgaatttatc
    20821 aacaatcgat ctttttgaga tcaatgaagc atttgcagct tcttctatcg tagttagcca
    20881 agaactgcag ttagatgaag aaaaggtcaa tatatatgga ggagcaatcg cattaggcca
    20941 tccaattggt gcaagcggtg cacgtatctt gactacatta gcttacggat tgcttcgtga
    21001 acagaagaga tacggtattg cttcattatg tattggtgga ggtttaggct tggcagtctt
    21061 gttagaagcg aatatggagc aaacacataa ggatgtgcaa aaaaaaaagt tttatcagct
    21121 tacgccatct gaacggcgca gtcaattaat agaaaaaaat gtattgacac aagaaactgc
    21181 gttgattttt caggaacaaa cactttctga agagctctcc gatcacatga tcgaaaacca
    21241 agtgagtgaa gtggaaatcc caatggggat cgcgcagaat tttcaaatca atggcaagaa
    21301 aaaatggatt ccgatggcaa cagaggaacc atctgtcatt gcagctgcaa gtaatggtgc
    21361 aaaaatctgt ggaaatatct gcgcagaaac acctcagcgg ctgatgcgag ggcaaatcgt
    21421 tctatcagga aaatcggagt atcaagcagt gatcaatgca gttaatcatc gaaaagaaga
    21481 gctgattctt tgtgcaaatg aaagttatcc ttcaatcgtg aaacgcgggg gcggtgttca
    21541 agatatctct actcgggaat tcatggggag tttccatgca tacttatcta ttgatttttt
    21601 agtggatgta aaagatgcaa tgggcgctaa catgatcaac tcgattcttg agagtgtggc
    21661 aaacaagtta cgggagtggt tccctgaaga agagattcta ttcagtattt taagtaattt
    21721 tgctacagaa tcattggctt ctgcctgttg tgaaatccca ttcgaacgct tagggagaaa
    21781 caaagaaatc ggtgaacaaa ttgccaaaaa aatccaacag gcaggggaat atgcgaagct
    21841 tgacccttac cgtgcagcaa cgcacaacaa agggatcatg aatggtatag aagctgtagt
    21901 ggcagctaca ggaaatgata cacgtgcggt tagcgcttcg atccacgcct acgcggcaag
    21961 aaatggatta taccaaggat taacagactg gcagatcaaa ggggataaac tagttggaaa
    22021 actaacggtg cctttggctg ttgccacagt aggcggagct tccaatatcc tgccaaaagc
    22081 aaaagcatct ttagctatgt tagatattga ttcagccaag gaactagcac aagtaattgc
    22141 tgcagtaggg ttagctcaaa acctggctgc tttgcgtgca ttagttacag aaggaatcca
    22201 aaaaggccac atgggacttc aagctcgttc attagcgatc tcaattgggg ctattggtga
    22261 ggaaatcgag caagttgcga aaaaattacg tgaagcagaa aagatgaacc agcagacagc
    22321 tatacaaata ttggaaaaaa tccgggaaaa ataaaaaagc aatatcttta gaagaataaa
    22381 gcaagattta cagttatttt atttactgca gatcttgctt ttttctctat cagaaaatat
    22441 gtttattatt agaatttttt atagttaagg ttaggcatct taagaaatca ggcaactcca
    22501 agatatcttc aggtagaaga gaagtcgcct aacattgtag gatcataggt gatttgttgg
    22561 acaggtcaag ctgatatttg agcctgatgt tagaaggaat ttctttttac ttgctgtaga
    22621 aattattata tgactaaaaa ggctgggatt ttaaatccca gccttttatg acaaatagaa
    22681 tttttatcga tcagtaatcg ctgtcgctgc aaattgttcg atcaaaggaa tcaaagtcaa
    22741 tttgactttg tatttccgtt cgagtgcttt ttcgataacg aactcatttt cagtagtcat
    22801 agaaatagtt gctaatttac gaggttcatc agcatttttt tcattaataa ctacttcatg
    22861 ttcattttgt tctgtgtaaa atttaggata ctcagcaacg gcttttgaaa aatcagcaaa
    22921 acttaaagcg gcattcgtat cagttgagcg tgtattttca gttgtgtccg cttttggttt
    22981 ttccgttttt tcttctggca cactgactgt ttcttctgta actggtgtcg caacagcttg
    23041 cgcagttggt tcatcgtttt tcaatcgttg atcattccag taagacacga tttcaataag
    23101 aagattgtca cggaatcgtt cgaaaatccc gttttctcct gaacgaattc tctttaatac
    23161 agtattgaag ttgtcatcga ttggcacgtt gatccgtgta tcttcatttg ttttcccagc
    23221 taatccgatc gttaagattt gacggttttt gggatcaagc atgacttgta acggcaaacc
    23281 ataacgtcgc aagctctctt cttctagaga acggaataat tgtcctctta caccatccaa
    23341 tgaaggacgg aattgattta ataatttttg tttctgtttt ttcttcatat gttctctcct
    23401 tcgctcatac cctttcgatt ataagaaaaa aacagccaaa tgaaaaggag taatgcaaaa
    23461 aaacttgcgt atacgcaaat tacaacagaa aaatttgcgt attttcccgt tttttgaaag
    23521 aaaaataaa
//
LOCUS       DACDUU010000045        23400 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.045, whole genome shotgun sequence.
ACCESSION   DACDUU010000045 DACDUU010000000
VERSION     DACDUU010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.045"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(82..2226)
                     /locus_tag="IWL30_001754"
     CDS             complement(82..2226)
                     /locus_tag="IWL30_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9706510.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(2583..3956)
                     /locus_tag="IWL30_001755"
     CDS             complement(2583..3956)
                     /locus_tag="IWL30_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9706511.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(3956..4351)
                     /gene="rbsD"
                     /locus_tag="IWL30_001756"
     CDS             complement(3956..4351)
                     /gene="rbsD"
                     /locus_tag="IWL30_001756"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAP9706512.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(4379..5296)
                     /gene="rbsK"
                     /locus_tag="IWL30_001757"
     CDS             complement(4379..5296)
                     /gene="rbsK"
                     /locus_tag="IWL30_001757"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP9706513.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(5404..6417)
                     /locus_tag="IWL30_001758"
     CDS             complement(5404..6417)
                     /locus_tag="IWL30_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9706514.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(6498..7097)
                     /locus_tag="IWL30_001759"
     CDS             complement(6498..7097)
                     /locus_tag="IWL30_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9706515.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            7283..7984
                     /locus_tag="IWL30_001760"
     CDS             7283..7984
                     /locus_tag="IWL30_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9706516.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            7994..10705
                     /locus_tag="IWL30_001761"
     CDS             7994..10705
                     /locus_tag="IWL30_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP9706517.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGTIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            11156..12784
                     /gene="treC"
                     /locus_tag="IWL30_001762"
     CDS             11156..12784
                     /gene="treC"
                     /locus_tag="IWL30_001762"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAP9706518.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12844..14025)
                     /locus_tag="IWL30_001763"
     CDS             complement(12844..14025)
                     /locus_tag="IWL30_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9706519.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            14277..15800
                     /locus_tag="IWL30_001764"
     CDS             14277..15800
                     /locus_tag="IWL30_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP9706520.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(15869..16597)
                     /locus_tag="IWL30_001765"
     CDS             complement(15869..16597)
                     /locus_tag="IWL30_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9706521.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(16610..17890)
                     /locus_tag="IWL30_001766"
     CDS             complement(16610..17890)
                     /locus_tag="IWL30_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9706522.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(18031..18633)
                     /locus_tag="IWL30_001767"
     CDS             complement(18031..18633)
                     /locus_tag="IWL30_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAP9706523.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            18878..19084
                     /locus_tag="IWL30_001768"
     CDS             18878..19084
                     /locus_tag="IWL30_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706524.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            19396..20115
                     /locus_tag="IWL30_001769"
     CDS             19396..20115
                     /locus_tag="IWL30_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP9706525.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(20196..20879)
                     /locus_tag="IWL30_001770"
     CDS             complement(20196..20879)
                     /locus_tag="IWL30_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAP9706526.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            21097..23283
                     /locus_tag="IWL30_001771"
     CDS             21097..23283
                     /locus_tag="IWL30_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP9706527.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         7799 a         4209 c         4583 g         6809 t
ORIGIN      
        1 acggaataca aaataaatat aaagaaatag aataaattaa aaagggagtg tgacagtgtc
       61 acatcccctt tttaatttat tctatttctt taaaggcaga tcaagccaga atgatttgtc
      121 taaacttaac aaggttagta caggaacagt tgcagcttta actgtacgag tagttgtttc
      181 tggtatctgt cttgccgtaa aattgttcca caaatcccag attgaagcga cacggaagat
      241 ggaagaagcg ttgttgacgc gttttatcgt tgaaaattct aatcctgtga ttggcacgcc
      301 atctgttgtc catttgattg gcacatcgaa tcgcttagac atattgagga tttcagcgtt
      361 tgcttcttca gttgttgctg cttctttttt cggttcatca tattttgtca ggtcgtacgc
      421 ttcgatgatc gcgttcaatt tttgtgaata ttggcgatct gtagcataac gtccttccaa
      481 atatttggtt gcttctttgt agtcttttgt ttccgacttc caagttggtt tgtagaaatc
      541 aggattgcca gtcagtccat ttttgatcaa gtcggcatag tcttccaaac tttccttgta
      601 agaaggatat ttacggaatt gtgcgcggat ttggtagctt tgtccggaac tatcttgttc
      661 aagtgtattg aaggtcacgc tgcttccttt atatgatcct ttgataccaa aaaggttatg
      721 atttggttcg cttgataata agctgtttcc agatccactt tcaaggatag cttgagccat
      781 catgactgat gcgtagattc cttctttttg cccgatttcc tgtgcatcat ctgcaatctt
      841 tttcaagaac tgatcagttg tttggttttg actgaaatgg aacgagccat ctaatccatc
      901 taaaatagat gagccgcctg tcagcgaacc tgaagcagca ggtaaacctg ctaagtttac
      961 tcggacagca aaatcaggcg tgaaatctga aatagaagtt actcgaactt tatctcctgg
     1021 ttgaggcgca tggataaatt gtccgttccc aatatagata cctacatggt gagtaacacc
     1081 ggaagaaggt ccccagaaga cgaggtcgcc aggttgggcc tgacttactg gaatctgcgt
     1141 acctgcatat tgctgttcgc ctgtccagcc accgatataa tgaccggttg ctttcatata
     1201 tacgtaataa gtcaaaccag aacagtcaaa agtatttgga ccttttgctc cccatacata
     1261 tggttttcca agatgcttga atgcttctcg aacgattgct tgttggacat tcgttgtatt
     1321 tgtaggcaat acagcatcaa ttggtgattt tacaacaaaa tcagaagaag cagaagatgg
     1381 attctctata ccgctttgat ttgctgtatt tggacgagat gaagcattgt tcgttccgcc
     1441 attcgatgaa ccattgtttg gatggtttgg ttctctctga gtatttccat tgttattcga
     1501 tccagatggg tttgtttgat tcgaagaatt cccgctgttc gaattattgt tattattgtt
     1561 tgaattattt gaaccattat tattgttgtt attacccaca tctggtttgt ttggcgtttc
     1621 cggatctggt gtactaggga cttcttcact aggatcagtt ggtttctctg ttgaagtatc
     1681 cggtccagct ttaaattctg cagtaataga taaattgttc gtacctactg taaaggtctt
     1741 tgcatcataa tttacttctt tggcaccatt aacagcgact gaagcaattt gttcaccttc
     1801 agctggcgta atagtgtaag ttaccttcgt ttccgctaat aaaccttcta ccgttccact
     1861 ttccgtaata tccaaaggtt gctgttcagg ggaatccgct gtcgaagcag tcagttggat
     1921 cttaccaatc aaagaaggag caattgtaag ggaatatgtc tcggctggct tttcattttc
     1981 tgatggatca gccggatcaa tttcagttgg ctcggtcgat cctgttgttg agttagttgt
     2041 tccagttggg tcttctgtaa aagaatccgt tgtttcacta gtagctgagc tggatgtagt
     2101 cgtatctgtt gtgactgttt gatcagctat ttgattatct acggcaaata cggtattggt
     2161 cgacaaaaca gacggagcaa tcagccaagt agcagctagc aaacgtatcg tttttttatt
     2221 tttcataata aactagtcct tttcctttca ttgattttct gtcttattca gcattcatta
     2281 gacatttttt ggaacagata atcgtgtcga aaagcttttt cttttcataa atctgtctgt
     2341 gtattctaaa taaaacaatt tgtaaataag caacgagcat tcattcgttt ttctttgacg
     2401 ttcattttat tgcatagacc acctttgttt ggggataaac aagtggggat aaaaatggtt
     2461 atgcatatgt aaattatacc aaagtttgga aagtatcaca aaaatttata aaaataatga
     2521 tcctaaaaag aaaaagactg aaatatgtga taaacatctc atacttcagt ctttttcatt
     2581 aactattcat aagacattag gtcttcatca aacccgctaa cgagagtagt gatcattgct
     2641 acgactagac taacagccag tcctgctaag taccagaaaa aagtaccgga aatgaatgca
     2701 ggcaatgtgg tcagactacc aaatacaaaa gcgatcgctt tcacttgggc aaatcccatt
     2761 acagctccac cagcagctgc accaatgcaa gcagcaacaa atggtttttt caatgggatc
     2821 gcaattccgt aaacagttgg ttcggtcact cccgcaagta aaatcgaagc aaaagcagaa
     2881 ccggagatcg aacgggtttt tgaacgttta cttcgcagaa atacacctaa agctgcacca
     2941 gccattccaa aattggctgc tcctgtcaac ggaacgattc ggtcgaaccc ataagcagag
     3001 atattactga tcatgacagg gtttacagcc cattggatac caaatattac caaaatattc
     3061 caaccaccag cgatgattgc gccactgatc ggtccgttaa tatccatcat ccaagtaatt
     3121 gcggcagcta aaccttcgcc aaggtaaaca cccgcaggcc cgaataccat taacgtcaaa
     3181 ggaaccgtga taactaatga aagtaaaggt acaaatacta attgcatatt ttcgcgaata
     3241 tattttttga ggaatttttc aacaaaagaa taaaagaaaa tacctaaaat cgccggcatg
     3301 acagtagacg tatactgcat cagtactaca ggaatcccca gaaaatcagt agctgctttg
     3361 ccactgtctg aaagtaaaga ggtcagattc ggttcaatca gtgctgcagc aatgacagcc
     3421 gcaatatatc cattcacttt aaacgttttg gcagcagaaa aagcaagaaa gattggcaga
     3481 aaataaaaga cggcattcga cgcagcagac aaaatatgat aagttccgct tgtttcacta
     3541 agccagccaa tcgtagtcgc taaaacaacc agacctttta atataccaga accggcaaac
     3601 agcggaatga tcggcgtaaa gatttccgat atcaaaccaa atagtttttc gatcggattt
     3661 agcttttctt tcggagctga atgatcgatt tgacgcgtca attccttgta agcaggagcc
     3721 aatccctgat ccatgattat ttctgcatat ctatcgagta aaatgaaata cttaatggaa
     3781 gggatttgct gttttaattt ttctgcattg gctactggct gttttttcaa tttgatacga
     3841 agccgagtcg cacatggaag gacagaatca atattgtctt ttcctcccaa ctgttcaatc
     3901 agcatatgaa tctgctcgga cagtaccatc tgttcaggat tcattgtcgt ttccattaga
     3961 agaccactcc actcactaat ataatattgg cgtaaggtgt catttcgcct gtacgaataa
     4021 atgcatgaca gttattcaac tcttttttca tttcttcatg tgggataaag ctaatcggtg
     4081 tatctggtaa caataatttg attttttcca atatttccgg gttcattgtc tttatttctt
     4141 ccgccaattc aatcgattgt acctcaagtt cttctaaaac gttttctaaa acagataaaa
     4201 aacttggaag accgtttgag acagccaaat ctattttttc agtaccaaag gggacaggca
     4261 tgcctgcatc cccgatactt aatttatcaa agtgccccat ttgtgcgatt actcgtgaaa
     4321 tatctgaatt gattactttt ccttttttca tgtttgtttc ttcctctcat tgttaatttt
     4381 agactgctga ttgttctaaa gcttcgcggt aagggataga aggttgcgca ccataagact
     4441 gcacagcaac agaagaggct agatttccat aaaggatcgc ttctttcaag ttactaaggt
     4501 ctggctgaag tgtgctgctt aatgcgccaa taaatgtatc acctgcggct gttgtgtcaa
     4561 cggcatccac tttaaaagca gggacgatac cgtgttcttt ttctgtatga tagaaagcgc
     4621 cggcactgcc tagtgtaatg attactgttc caatacctaa ttcatggagt ttttcggctg
     4681 ctgcgaccaa actgttatga tctgtcactc tgattcctgt aatgatttct gtttctgttt
     4741 catttggtat aatgatatct gtcttttcaa gtagttcagt aggaatcgtt tcacgagctg
     4801 gtgcaggatt caagatcgtt gtttttcctg cgtcttttgc aatagaaaaa gctaggatcg
     4861 tcgcatctaa gctactttca aactgagcaa taataaagtc actgtttgta atcagttgct
     4921 tgttttttag gacttcttgt tcatggaaag cattatttgc ccctgcatgg atcaagatac
     4981 tattttctcc tgctgcgtct accataatca ttgcttgtcc ggttgtggcg ccttggattt
     5041 cagctatccc agatgtatcg atgttttctt ttgtcagtag atctaataac tgttgtcctt
     5101 cgctgtcagc cccaacgcct ccaataaaag atgtccgtgc gcctgaacgt ttggctgcca
     5161 ctgcttggtt ggcaccttta cctccgccag cgtgaaaaat ctcatgcgca tgcatcgttt
     5221 cgcctggctt gggcatgttg gttaaacgaa gggtagtgtc catattaata cttccaatta
     5281 ctgtaaccgt gttcatttta caaacctcca actatctcat ttattttgat gggaaaatta
     5341 ttttttaaaa cgttttagta aaacgtttta atagttcgat aaaaatatag cacaccttga
     5401 aagctatttc aagggtgcag ttgaaaatct tttttctaat cgtacaggta atcttttttc
     5461 ttcccattct ttttctggga attggatccg atctaacaat aacttggcag atgtttgtcc
     5521 caattcgaag attggttgtg cgatcgttgt cagtttcggc ttgatatact cgcacatatc
     5581 tatattatca tagccaataa tgctgtagtc ttcagggatg ctttttccag cttcctctaa
     5641 tccgcgatat aatccaaaag ctaattcatc atttaaagca aacacaccag ttgcagcaga
     5701 atcaagaagt tctgctgtga tctggtagcc accttgtttt gagaactgtg taggtaagag
     5761 gatcagttgg tcgtgaggga tttggtaaac atcaagagca gatttaaacc cctcaatccg
     5821 agcgtggaca ttttccggtg gattttcagg atagacaagg gcgatcgttt gatgtcctag
     5881 actaagcaag tgcatgcccg ctaggtaacc accgcgaaaa tcgtctgttc gtacggcatc
     5941 actaaatcct tcagattttt tctgatccaa aacaatgaag ggccggcctt gttttttcaa
     6001 attttcgttg attgcatctg tagagacagc agaagtcgcg ataatgaatc catccacacc
     6061 tcgacgtgtg agctcggcta gatattcgat ttctttttga tgatcagaat cagcattaca
     6121 taatatcgtg acgaaattct gcttatacaa gatatcctca attcctcgca tcagtgtact
     6181 gaagaatgga ttcgtgatat caggaacgag tacaccaatt gttttcgttt ccttggtaat
     6241 catttgtcta gcaaaatagt caggctgata gcctaattct tctttggcag ctaaaacttt
     6301 ctgaacagtt ttcggactga atttctccgt atttccattt aatatcaagg agacagttgt
     6361 gattgaaacg ccggaaagtt tagcgacttc tttgattgtg atcttttttt tcaccatgtt
     6421 catactccta tctaaatcct gtggttattt tcccatgaaa tcaaaaagaa gtaaatgaaa
     6481 atcacgtgtg ccagtactta tttgctttgt tggatagctt cgattatttg tttcgttgag
     6541 cgtactcgtg cagttttcgg aaatatcttt tttatgaaca attgatggga ttctttgtca
     6601 cgatcactgc aagcatcttc agcaagcacg atatggtacc cgtgttgaaa ggcatctaat
     6661 gcagtagcat atacaccgtt tgttgtcgct acaccgctta aaatgatagt ttcgattcca
     6721 cgccggcgaa gttgaagatc aagagaagta ccaaaaaaag cacttggatt gtattttgtg
     6781 atagtcaaca tattatctgt atctttcaaa gaatccttca aaagtaaatc agtgaattct
     6841 ggaggtacag aagcagtcat tccacccata tcagtttgtt gactcaaata agggaaagtg
     6901 gagatgtcta cgttcactaa aactttcaag gcagcggtac gtttcaatgt tcttactaac
     6961 aaatcattgt ttgtcagaat agatgctgca gtgtgtggag agagtgtacc gttatttacg
     7021 atctcttttt gcatgtcgat cgcgacaaaa gcagtttttg ataaatcaaa aatcatattt
     7081 tcctttaaat cattcatgat gttccttctt tcaaataaca atgtttttta tgtaagtaaa
     7141 tcataaatcg tttatttgtc aactaaatta aaggattcct ttctttagtc tagagaaatt
     7201 cagtggtaaa ataaataagt gtgagaaaac gcattcgatg catagatata gcgagaaata
     7261 tcgtgaaaat ggaggaatca gagtgaagta tgtcgaagta aaaaatgaat acaaacgata
     7321 caaaatggga gaaacgacca ttacagcaaa tgacgatatt tctttttcaa ttgaaaaagg
     7381 cgaattagtg attatacttg gtccaagtgg agcaggaaag tcaacgattc ttaatatttt
     7441 aggtgggatg gatacacctg atgaaggtca gatcattatt gatgacacag atattgcgca
     7501 attttcagat aaacagctta ctgcttaccg acgaacagat gtagggttcg ttttccaatt
     7561 ctacaatctc gttcctaatc taacagcaaa agaaaatgtg gaattggcaa cggaagtttc
     7621 tcctgatgcg cttgatcctg tagaggtatt gaggcaagtg ggcctagctc atcgattgaa
     7681 taactttccg tcccaattat caggaggcga acagcagagg gtttcaattg ctcgtgcact
     7741 agctaaaaat cccaaacttc ttttatgtga tgaaccgaca ggcgcgctgg attttgaaac
     7801 gggcaagcaa gtattgaaat tattgcaaaa cgctagcaga caacacggca ataccgtttt
     7861 gatcattaca cataattctg ctttagcgcc catcgctgat agagtcattc acattaatga
     7921 tgctaaagta aggtctgttg aattaaatga tcatccttca tccattgatg agattgtatg
     7981 gtagggggat tttatgaaaa caagaacata tcttaaagca ggttttcgcg aaattcggca
     8041 atctaaagga cgattcattg caatcatcct tattgtcatg ctaggaacac ttttatttgt
     8101 aggtgtcaaa actgctggac ctgtcatgca aaaaacgatg gatcactatg ttggtacagc
     8161 aggactttct gatttgcaaa tcgtttctac tggtggcttg acacaaaaag atattgctca
     8221 agcagaaaag atcccagatg caaaagtaga aacaggaaaa cagatctatt attccaatac
     8281 tggcaaaaac gaagtgattc aaatattttc ttataacaaa aataacaagc agaacaagct
     8341 acaattaact gatggaaaac tcccggaaaa accaaaccag cttgtattgg atgaaaaagc
     8401 ggaagatgaa gggtataaaa tcggtgatat ttatcagatt gattcagatg aattgaaaga
     8461 aaaagagtat accatcacgg gctttgtccg ttctccattg ttcatcaaca atttggagag
     8521 aggatacgcg aacgtcggaa atggtagcgt cgattatttt gtctatttac ctgaatccgt
     8581 ttttaaaaca gatatttatt ctgtcttata tcttgatttt acaaacgtaa agaatttgaa
     8641 tacgtacagc aaagcttaca aagataaaat ggagcaaaat caagaaaagg cagaaaagta
     8701 tttacaagat cgacccgaag aacgcttaga agagctgaaa aaatcggcca acgaagaatt
     8761 agaaccggca cagcaaaaaa tagcggatgg caaagcacaa acaacgcaag caagaacacg
     8821 gttagaagat gctaaaaaac agttagcgca acagtcagca actatccagc agcttcctga
     8881 agcgcaacga acaatcgctg ttcaaacatt atctcaacaa gaagctcaac ttgctgaaca
     8941 agaaaggcaa ttagttgaga aagaacgtga actggcctct gcagaggaag aattgaatcg
     9001 aaatgcagaa aaaattgaaa atttaactaa accatcatac ctatacaatg aacgttcaga
     9061 aaatgtcggt tttcaagaat ttggtgatct gtctgatcgt atcgctgcga ttgccaatgt
     9121 ttttcctgtt ttctttttct ttattgccgc attgatcacc tttacgacaa tgacacgtat
     9181 ggtagaagaa aaccgcaggg aaatcgggac attaaaggcg ttaggttata cgaaagttga
     9241 aatcgcaaaa aaatatgcaa tctatgcaag tctagcctca gggataggta ttgttttagg
     9301 aacgatatta ggaacgaatc ttttgccaag gatcattttt gagctgtcta acgagcggta
     9361 tgatattggg agtgcgctta ttttttatga ctggccgccg attttacaag cagctgccgc
     9421 attcttcatt gccgcatttg gcgctgcaat gatcgtttta ttcaaagatt taagagaacg
     9481 gcctgctgca ttgcttcaac cgaaagcacc gaagccaggg aaacgcattt ttcttgaata
     9541 catcactccg ctgtggtcac gccttagctt taatcaaaaa atcagttacc gaaatctctt
     9601 ccgatataag tcaagaatgc ttatggcgat tatcggaatt gctggatgtg ccgggctgat
     9661 ggttgcaggc gtgggattga aagattctct aagttctgtt tcagccaaac aatttggaac
     9721 gatcattgac tatcaagcaa tcgttacatt ggattcatcc acggaagata gtcgtcaaaa
     9781 agcagcggat atgctgcaga agcaagaaaa agtaaatgag atcctagcta ttcatactca
     9841 aaccgtagaa ttgagaaaaa aaggacatgc ttcgcaaagt ctgacagtga tcgtaccaag
     9901 taaaacagct gatttggatc gttttatcca tttaactgat cttgaagaga acccacgctc
     9961 cttgcctaaa acaggaatcg ctattacgca aaaagtagca gaactatttg acttatctac
    10021 aggagatcat atttcgcttt atgatgaaga tcaaaaagaa tggaaagtcg aagtaaaaga
    10081 aatcgtgaaa aactatctag gtagctttat ttatatgtcc cctgaatatt atgcttcagt
    10141 tactgatcaa tcgatgatgg ataatgcatt tttgctgcgt acagataaga tgacaacaaa
    10201 agaagaagaa atgctttccg agaaactgct tgccacagac gctgtgacaa atacgtcttt
    10261 catgtccaaa caaatcgaaa cgcaagaaga atcaatggcg aacttagact cggttgtctt
    10321 aatctttgtc gtattatcag gactgctggc ttttatcgtc ttgtataatc taactaatat
    10381 caatatttcc gagcgtgtac gcgaattatc tacaataaaa gtccttggat tttttgataa
    10441 agaagtcacg atgtacattg ttcgagaaaa tatcatcttt actcttttag gaatcatcgg
    10501 aggatttggt gtaggatata tattgactga cttcatcttg caacaagcgt ctatggagaa
    10561 tgtcatcttt ccattagtga tcacatggtg ggcttatgtc ttatcagcag gattaactat
    10621 cgtatttacg gttattgtga tgattgttac ccatttcaaa ttaaagcata tcgatatgat
    10681 tgatgcgttg aaatcgaatg aataaaaaca gaggttgtga aatcagcggt ttgacctgag
    10741 tattagagaa acaaacggca aaatagtttt tcatattttg ccgttttttg agctaatacc
    10801 gaaggacagc tgattgaaca ccatgaatca gaggttgtaa aaaagccgtc ctgcatcgag
    10861 caaaaagaat actcaaataa aattagctcc tcatgtttta catttgagaa gactgattgc
    10921 cgtagataaa agcttttgaa caccatttat agaagaaaca agaatatttt gctattttaa
    10981 acaagaggat agacaaatac atgtctgatc tcttgttttt tattgggcaa cagacctctt
    11041 agaatgtata aagataaatt ttacaaacgc tttcggttca gttataataa taaatgataa
    11101 cttgtatata taagttgtgg taggtaataa atcagttgaa tggaggtaaa tatccatgac
    11161 atttaaagac aaagtcattt accaaatcta tcccaaatct tacaaagaca gtaatggtga
    11221 tggaattgga gatttaaaag gaatcaaaga aaaactcccg tatctgaatg atctaggcat
    11281 cgacatgatt tggctaaacc ctatttatcc aagccctcaa aaagacaacg gttatgatat
    11341 ctctgattac acagccatcg atcctatctt tggtacgatg gaggaattcg aacagctaat
    11401 agccgaagca aagaaatacc ggatagaaat catgctcgat atggtgctga atcacgtttc
    11461 gacagaacat gaatggttca aaaaagcact agcaggggac cagcattatc aggacttctt
    11521 cattttaaga gaagagccaa cggattgggt atcaaaattt ggtggaagtg cttggtcgcc
    11581 atttggagat acggggaaat attatttgca cttatatgat cggacacaag ctgacttgaa
    11641 ctggcggaac caagcagtaa gagaggaact attcaaagtt gttcgttttt ggatggaaaa
    11701 aggggtaaaa ggattccgtt tcgatgttat caatgttatc ggtaaagacg aacaattgaa
    11761 aaataataaa gaaaatgatg gcaaacctga atatacggac aaaccaatca cccatacgta
    11821 tctaaaagaa ttgaaccaag caacgtttgg gacggattcc actatcatca cagtcggtga
    11881 aatgagctct acgacgatcg aaaattgtat cttgtataca gaacctagta gagaagaatt
    11941 atcaatggtc tttaatttcc atcatttgaa agtcgattat gaaaatggga ataaatggtc
    12001 tactcctcca tttgattttg cagaattaaa gcgactattt catacatggg gagaaaagat
    12061 gagtgagggt aacggctgga atgctctatt ttttaataat catgatcagc cacgtgcgct
    12121 caatcgattt gtagctatcg atcagtatcg aaaacaagga gcagagatgc tagcaaccat
    12181 gatccatttg aaccgtggga cgccctatat ttatatggga gaagaaatcg gcatgatcga
    12241 cccagactat tcatcgattt ccgactatat tgacgtagaa agtttgaatg cttataagtt
    12301 actccttgaa caaggattca cagaaaaaga agcgtttcgt cggatcaaag caaaatctcg
    12361 cgataactct cgaacaccca tgcaatggtc agacacagaa caagcaggat ttactacagg
    12421 gaaaccttgg ttaaaagttg gaggcaagct agaagagatt aatgtagaaa aagaactatc
    12481 ttctgaagat tctattttgt cctattacaa gaaattgatt cggctgagaa aaacatatcc
    12541 aatcgttgca caaggagatt atcatgctta tgaagcaaat catccggaag tttatggctt
    12601 tcttcgccaa tttgaaggac agcagctgct tgtattaaca aatttctatg ataaagatac
    12661 aaggatcgcc atcccagaag gattcacaga agccaatatt cttatcgata attattccga
    12721 tactgttctt catcaaagtc tggagctaaa accataccag gcaattgctt tgctgaaaaa
    12781 ttaaaacaaa aacacaaaac gtcgttccta cgagctattc tcgtaagatc gacgttttgt
    12841 gttttattta aaataatctt cttcagaaaa tgaagagttt tcatactctc ctttatcgcc
    12901 ataaaattta ttgtagcgtt gtttgaattt acggaaaata aaagtaacga gtaatttgac
    12961 taacgtatag ataggaatac caaaaataac gcccacaatt cccattagat cacccatgac
    13021 aagtaaaacg attaagatcg taatcggatg gatctgtaaa cgatcaccca ttactcgagg
    13081 aatcaccaag tcaccgtgga gaagttgtac ggcaaaccag acaatgacaa atttgacagc
    13141 cataaaagta gagtcttgga atgcaatgat caaacctgga aagaaggcga taaacggccc
    13201 aatatatgga atgatacata acaacccagc agaaatagct aaaacactag caaattctaa
    13261 tccgatcagt aagaaacaaa cccagtagac tgctccaagg ataaatgaag caatgatttg
    13321 cccctttaga aatgaaccca attgttgatt agcaatcttt gtcaaatttt taaaatctgt
    13381 acgaaatgct ggtgggacaa tcgctaaaac tgactgataa aatttttgag gatctctcaa
    13441 aaggaaaaaa gcgacaattg gaccagtaaa taaagtaata aaaatagctg taacagttga
    13501 aaaaatgttg ctgatccgtt gtgcgccact ttcccaataa tttccaataa atccagctat
    13561 atcagacgtc acgtcgtcaa tagaagcgaa aaattgatta aatgaatcgg caaaaggtgt
    13621 tttctccaaa aattgtcgga gactctcttg aaaatcttga aacaaatctg gcaaactact
    13681 gatcaatgtc tttgcctgtt cttcaagaaa aggaataagc catatgcttc caagccccaa
    13741 taaaaataaa ataatcacat aaacgataat gactgacaag ttcctcgaaa ggtgcttgct
    13801 caaacgtttg atgactggtt ccaaaagata atacaagatc agtgcaaaca ataatggggg
    13861 caagatagta gcgataatga ttccgacagg cttgaatata aacgagatct gatggaaaaa
    13921 ataaatgatc agcgcaagta agataactat tcctaaaaca tagtaagagg tttttcctcc
    13981 taaaaagcgg atcaaacgat tcgttgtatc tgtttctttt ttcactcttt ttcctccttt
    14041 atctataaat tcattataga caaaagcggg tataaaagga aaatgataga ggttgattgc
    14101 aaaaatatcg tcaatcattc atataaactt tctcatcaat taaaataaga aaacgcttga
    14161 cagttctttt aaccatatga taaattaaat cctgttgcta aaacgtaact attacggttt
    14221 gcgaaaagcg aatcggaaca ttcaaatgaa agaagatgag aagaggagtg tcataaatga
    14281 aacgattgat aggtatattg gccatgctgg ttttagcagg tatgttaact gcttgcgggg
    14341 caagtgggaa agccgaggac agtaaagaaa aattgagcgt gatgacgacc ttttatccca
    14401 tgtatgattt tacaaaagca atcgtcgggg atgaaggaga agtagagtta ttgattccag
    14461 cagggactga ttcccacgat tatgaacctt ctgctaaaga catggcaaag atccaagata
    14521 cagatatctt tgtttataat gatgaaaaca tggaaacatg ggtccccgca atccaaaaaa
    14581 cattacaaga aggcaatgtc catacgataa aagcgacaga aggtatgctt ttacttccgg
    14641 gaagtgaaga aggacatgat catgaccatg aacacgggga agaaggccat acacatgagc
    14701 tagacccaca tgtatggcta gctccaagtt tagcaatcaa gcaggtagca aacatacgtg
    14761 atcagctgat tgaagcttac ccagaaaaac aggaagtatg gacaaaaaat gcagcagcct
    14821 ataccgagaa acttcaagca ctccatcagc tttatcaaga aacctttaag caagcaaaac
    14881 aaagatcttt tgtcacgcaa catgctgcat tcaactatct agcgttagaa tatggattga
    14941 atcaagtatc gattgctggt ctttcttcta gtgaagaacc gtctgcggcg agaattgctg
    15001 aactgaaaca ctttgttaaa gaacatggaa tcaactatat ttattttgaa gaaaacgcca
    15061 aagatagtat tgccagaacg ttagcaaacg aagcaggtgt atctttagaa gtattgaatc
    15121 ctttggaagg attgacgaat gaacaaatag aaaatggaga aaactatctt tctatcatgg
    15181 aagcaaacct cgaagcgttg aaaaaaacga cagagacaga aaacccgcta gaagatactt
    15241 tgacccccaa aaaagaaaag agcgtctaca acggttattt tgaagacgag gatgtacaag
    15301 atcgctctct ttcagattgg gcaggaactt ggcaaacagt agatacgtat gtagaagatg
    15361 ggacttttga tcctgttttt gaatataaag aaaaattgaa tcaggataag acagcacaag
    15421 aatataaaga atattatacg aagggctatc aaacagatat tgcttcaatc aagatcagtg
    15481 agaaagagat ggcatttacg tttaagaatg gaacaacgaa acgttcaaat tatcgttatg
    15541 tagggaaaga aatcttgacc tacgaggcag gaaaccgtgg cgtaaggtat ctattcgaag
    15601 cagaagataa ggaaagtggc gcttttagat atattcaatt cagtgatcac acgattgcac
    15661 cggctgactc cgaacatttt catttatatg caggaaatga aagtcaagaa gtattgctga
    15721 aagaaatgga gaattggcca acattttatc cagatacaat ggatggacgt gaaattgcac
    15781 aggaaatgct tttgcactga taggcaaaaa agaaccgaag ggtttggatc ggctggtcat
    15841 ttagtcggtc caaactattc ggttcttttt atgaaatcaa tttctttaat ccacggatag
    15901 aggctgggat ggtctcgccg tttgccaacg tgacttctct ggttttttta ttaatcgacc
    15961 gcacattttg tgtattgata acataggatt tatgacaacg gataaaattc cgatgcattt
    16021 tctcgatttc tttgatcttc tcatagaatt gtaattgggc atgagtcaaa tggacttcca
    16081 gtttatgtgg cgctcctagc gaagtagaaa aatattggat gtctttgatc ggtaaaaatt
    16141 tgatctcatc attaaactct attttgaaga tatctttttg gatttgggac atttgaaaat
    16201 cttcgaaaat tttgctcagg atatgctcga cattttcttt taaatcataa agatcttctt
    16261 tgacgatata atcagtcggt tcgatgttgc tttttaagat tttcatcgac atttccgtat
    16321 gggaagtcac atagattatt tttcctaaag gatctaattc acggatcaat ttaccgatcg
    16381 ctacaccatc catttcactg tcttctagtt cgatatctaa aaagtaaata ccaaaagaag
    16441 aagttctgat cttggctgca ttgagaagtt cttccggatc agaggtagaa atatggacat
    16501 aactgtctaa gttttcaatc atgattctat ttttgatcac cgttgataaa acttctaatt
    16561 gttttggatc gtcttcacaa atataaatgg gaatcatatc attactcctt tattttattt
    16621 gtatataaat cgtttgagaa aaacgctggt tttcaattga tgtttcaagg aaaagataag
    16681 gattggtctt tgtcaattca tccaagataa acaagccaag gccttcgttt tttggatttg
    16741 tttttgttga gaaccctttt tgtttcaact ggtttaacgg taatgtattt gttgttgtat
    16801 tgatcacacg aatcatcacg gaatgattct tcttgagtat ggtcaattcg atttttttct
    16861 tctttgcagc actagctcct tcaatcgcat tgtccaagat aataccaagc atacgtacaa
    16921 ggtttaccgt atgtttcgta tccagttcga ttggctcagg aaaatgaagc tggacgatca
    16981 accctttttc ctgtgccatc atgagtttta atgacagtaa actacggatc tcaggaactt
    17041 tcatattatc caacatactt agattggccg gaaaaaggtt catcatctgg cgtgtaggta
    17101 atatcgtttg atcataataa gaccgtaatc cttctaagtc gccttctgaa atatagctgt
    17161 gaagagaaaa aagcagattt tggtaatcat gtttgaattc tctcaagtct tgatagtttt
    17221 tttctagatc tttgatataa tcctgttgca tcctgacttg ttgctctaat aactgatcag
    17281 ctaatttctt attagagatt ttccaataat aaaccaagat gatcaggcca acacacagat
    17341 atagaatgaa aaaactcgta ttcaaagcta agacatcagg tgtttcacca gaaaaacggg
    17401 tataaaaaat aatgatatag tatgtaaata atatcaatgt cccgatcgta ccgatcatac
    17461 ggcggtccac agtgtgaaac caagtctcta acgatttttt taaacaaaaa gctaataaag
    17521 cagataaaaa agcggataag acaatgtgaa aatactgcaa tgaatctcct ggttcaataa
    17581 ccgaagattt caaaacaaaa aaatcgccga tgcttgttaa gtgatctcct aaaagcgacg
    17641 tcaataaccc aaaaggaagc gcgtaaacca attgttgacg cgaagtcgaa aagaagaaaa
    17701 aagtaagcat cagcattcct atagcaatta gagcagtaaa aatccctaaa atcgaataga
    17761 cccatccaat caaaatacaa gaaatcagaa tgactcccgc aaacagatat ttgtatcgga
    17821 tgtgatggtc aatcaataag atacaaattg tgtaaatcaa atattgcaac gctatggacc
    17881 ataaaaacat aaagtacagc tcccattgat ttttaccaaa ttatagcaca attggcgtat
    17941 tattttttta atttttcagg gattttgaat tcagaactca taccaaagca gcagccagat
    18001 acaaaaaagt cagataagtt agttaattgt ttaatgattg tttttttcat gaaagattcc
    18061 tccaaaattc atcaagtagt ttgtaatagg aagcaacgta gctatttcta aaacaatccc
    18121 aagtagcaaa taggtagaaa agtaagaagc tgagaaaaat aacgtaatga gagaaaaaat
    18181 tagaaacatg ctgatcgcct tctttaataa tgggggacgt ttctcgggtt caatgggatt
    18241 gattgctgta cctcttgatc catagcgaag cagtaacaaa aagcagctga ttaacaaaaa
    18301 gaccatcatt gggaaggaga tagtcaattg cagatgtttc atcaccaagg gaacgcccca
    18361 taaataaagt agtgtccaca gcagacaaac gaattcagag tcggaatgca atccataagc
    18421 atatctgcgg atgcagatat aagaaagaaa ggtgattcct gtttccgccc atgagcctgt
    18481 caccagagaa aagactagaa cgaccgctag ttttccaaga ttattcaata aaacttctaa
    18541 gacaaatttt acttggacat acaccatatc ttcttccgat ttgtctccgc tgaataaggc
    18601 ttctaataat ttgttcacaa ctttatcttt catctcatac gtacctccac cacaaatttt
    18661 atgctaaaaa aaataaaaaa cgtcttttgt ttcataaaaa gtggttttga catcaaaacc
    18721 gtcatgatct cctaaaaaaa tcttcgtttt ccaagaacct taccaaaaaa agtgacagtt
    18781 ttgatacgaa aagtactttt tatgacaagt ttgtgctttt tcgagtgatt tatgttaaaa
    18841 aattactgac cttataaaag gtgatggagg gattcttatg gaaaatgaac aattgtttga
    18901 agtcggcgaa acatatgcat ttaaatctga tttatttgaa tcaccaataa aaggaaaaat
    18961 agaaaaaatc tatgaaaata gtgtgatgat acgtgtcatg aaatgcgcag tagaagacaa
    19021 tgataccgct cataatctgc actggcagtt agttgtacgc aaatcaagta caatagaaga
    19081 ataaaaacag ataaaatgtt gagagcataa agaggttgtg agtctcccgt ttagctctga
    19141 gttacaagga ataatgttaa aaaacagctt ttcatgtttt atgaatattg ccttgtgacc
    19201 gaggtgttgg caggagaata ctgtttattt gaaaaaaggg ctgcgacaag acttttgtca
    19261 cagcctttat tttcatgaat aggagttact tttggctact tttagagaag tagcttttaa
    19321 ggagcagata aagtcaaggc ttttatttct ataaatgaag tagtaaaatg aagaacagat
    19381 tttattacag gaaggatgcc gataattgtg atcattgttg caggaacgat cggtgcaggg
    19441 aaaagcacac taactgaaat gcttgcccaa gatttagaaa caaaaccttt ttatgagaac
    19501 gtggaagata atgaagtatt gccgctattt tattctaatc cagaaaagta tactttttta
    19561 ttacagattt tctttttgaa caaacgcttt ttggcaatca aagatgcttt cagccatgat
    19621 gacaacgtac tagaccgctc catctacgaa gatagtatgc tgtttcattt gaatgcagat
    19681 ttagggcgtg tttctgaagt agaagtaaag cagtatgaag gtcttttaga aacgatgctg
    19741 aaggagttag aagaaatttc gccacaaaag aaaccagatc tactggttta tattcgtgta
    19801 tcctttgaaa ctatgttagc gagaatcaaa aaaagaggcc gggaatatga acagctagaa
    19861 caagatcctg aactctattc ctactataaa gaactcaatc gacgatacga agagtggtat
    19921 gaacagtttg atatatgtcc caaaatagtg atcgatgggg acaaatatga tttcgtagcc
    19981 gatccggcat gtggagaaca aatcgtacaa gaaatcaaga tgcgggcaaa gaaaatgaca
    20041 gaagaggcag atgcccagtc gatccgctac actacaaata acccgaaagc aaagggaatc
    20101 tttacaacta aataatataa aaactaagca aattgctcac tcgcagatat ttctctgtga
    20161 gcagcatctt gcttagtttt tttgattcat tgagatcaaa tagtgaaccg attcgtatga
    20221 taaaattgcc ataaatgaaa tgtctgagtg agggccatga tcaaaaataa aatggaaacg
    20281 atcaaaatcg aatgggcgcc aaacagatga tgacagaaaa caaggacaat agctcctgcg
    20341 ataaaggcaa gaacgacact tagatagtgg aaaaatcgat caaaatctgc tttttcttta
    20401 gaaatacaat aatcatataa gttcgttgct acccctttta aatttccagt tgtaaataaa
    20461 ttagtgtagg cacgcccgtt gattttatca aaagctaccc actggatcga tgcagtaaaa
    20521 gcaatcaaaa tcgttactaa tgcgggatgc tcatggacgg gtgtcaaact aacaaagaaa
    20581 aaaatcactg cttcataata tagaacaaac aagcgccaga aatgtttttc tttttttcta
    20641 aaataatcaa ttagatactt tgttagaaga atccctaaga aaaagaaaag ggtagacaat
    20701 acttttttac caacacttag ccattctccg tcaaatgctt ggatgatcga tagaatgata
    20761 ttgccagttt gagcagaggc aaaagcacca tattggatat aagtataact gtccatggct
    20821 cctccgataa atgtcagcaa aagtcctatt gtacgcccct cgtgaaagga agtctgcatt
    20881 tttttcatca ccttttatga atatttttgt cttccttatc ttagcataaa cgattgaaaa
    20941 aaaggaaaaa ttttcagaaa actaaaatca tatcgcttcc cattttcatt tttagaaagg
    21001 tctgttagta taaagaaaaa atagacaacg aaactttaaa taaccaactt gaaataaaca
    21061 aattcaaaga agaaagatgg gaaggaaatc aatcagttga atgaaaaaga aattctccga
    21121 ttacaaaaag aattgaaacg ctgggagaac aagtataaaa agcttcaaca agtctttcgg
    21181 ctacaagaag aaatcattgc tacctatgaa gcggcttatg gcgaagcacc cggacttatt
    21241 gaggaagaag tacaagtaaa agaaagtgaa ggaaacagct ctttctttaa aatgatggag
    21301 aaacgttcct ataatcgctt caatcaagaa caaaaagcag ctatcacata tgatatgaag
    21361 aaacatcttc gaatcatagc aggagctggg agcggcaaaa cgcaaacaat ctgtgcaaaa
    21421 gctgtttatc tgatgacgca aaaacaagta gacgaagaac gcatactaat gatcactttt
    21481 acaaggaacg ctgcaaatga actaaaaaaa cgagtagata atttcagcca gagaaaaacc
    21541 gctgtccata tcggaacatt ccatagtatc ttttttagga tatacaatga aatttgtcga
    21601 aaatttcctg aagtagcaat gcaaggaata caaggagact tttcaaagga tactgcacaa
    21661 aaagtgaatg ctgttttgca acagctgata cgtaaatata atctttatat atttgatcaa
    21721 tatggagaaa aaacgattgc ttctcggtta gactattggc agaatatgaa cctttcagta
    21781 gaagaaatgg ttcagttagt taaagaaaaa tatgattcca ttgataagaa tgcacgacag
    21841 ccgatatcag aacgactgta tggattgctc acggaactcc aagaacaaaa aagaagccag
    21901 caattgctag agttcaatga tgtactccaa aatctgaaaa gcgctttgga gaatgaggag
    21961 atccagcatt atatcggcca aaaatatgat tatctattca ttgatgaatt tcaagatact
    22021 aatccacttc aatgggaaat cgtgaaacgg atgacaaagg aaaacaggat caagttgatt
    22081 gttgttggag atgatgatca gagtatctat ggattcagag gatcagagcc agcatacatc
    22141 aagaactttg aaaaagaata tcctaccaaa actcttttct tattaactaa ttatcgttct
    22201 cgggcagcta ttgtccaagg agcaaataga ttgatcagct ataataaaaa tgatcggata
    22261 ccaaaggcga tgattccagc acaaaaagaa gcaggtgtta tggaagccta tttgttttct
    22321 gatactaaag cagaaagtca gtggctaatc aaacaaattc agacatttat tcaaaaaaat
    22381 gggaaatata aggaatcaat catattatac cgatcagctt cacaaacgca gcagcttgtc
    22441 caatctctgc tgaaaacgaa tattcctttt gtactggaag cagatagtcc atatgaaggt
    22501 atctttggta tcaagtcttt ccaacatttc tatcaaaaaa ttgttcattg gcaaacagca
    22561 ctgaatctgc aaaagaaaaa tcaggcctac cagcaattgc tccggcagct gatggcagac
    22621 tgctacctga aaaaaagtga atgcgatcaa tacttttctc ctaaatatca aaatatagcc
    22681 gttgcagatt atattttgtc gataagcccc aatttaaaat cgaaatctgc agaaatccga
    22741 gagtttgagg aagcgttgcg taaaacagac cagcatcaag aaatctattc gcttgttcaa
    22801 agctatcttc atctgccacg tatagctaag gaaatcgacc agagtgatca agaatggata
    22861 cgagaagaag taaagcagca tgctactttc agaagactcc aagccttatc gcaagaaacc
    22921 caagcgacag ctaaagaatt aaaaacccgc ttgattgctt acagacaagg aaaacttgat
    22981 gcactatgta tccagagcat tcacaaaagt aaaggtctta gttaccgaaa tgtctttttg
    23041 attggttgta acgaaggaag tttgccttat aacggagcga cagaaaagaa agtaatcgac
    23101 tttaatgaaa taaaagcaga accagtaacg acgatagaag aagaacgaag actattttat
    23161 gttgcgatga ctagagcaag aaatcgtctg tatctatgtg tacctcaaat gaaaaattca
    23221 agaaaattga aagtatcgag atttatcaaa gaaaccggga gtcgtgtgaa aaaaggaaag
    23281 tgatagaaga gagggtgtga cagaagtggt cagtttcaag aaagaagaag gaatgtacga
    23341 aaattgcttc tcaaattttt gtgaaatttc agcttatttt cgaagaagct atttctggaa
//
LOCUS       DACDUU010000046        22992 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.046, whole genome shotgun sequence.
ACCESSION   DACDUU010000046 DACDUU010000000
VERSION     DACDUU010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.046"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(111..566)
                     /locus_tag="IWL30_001772"
     CDS             complement(111..566)
                     /locus_tag="IWL30_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706528.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(720..1928)
                     /locus_tag="IWL30_001773"
     CDS             complement(720..1928)
                     /locus_tag="IWL30_001773"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP9706529.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2040..2372)
                     /locus_tag="IWL30_001774"
     CDS             complement(2040..2372)
                     /locus_tag="IWL30_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP9706530.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2520..3395
                     /locus_tag="IWL30_001775"
     CDS             2520..3395
                     /locus_tag="IWL30_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9706531.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3477..4058)
                     /locus_tag="IWL30_001776"
     CDS             complement(3477..4058)
                     /locus_tag="IWL30_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAP9706532.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4064..5323)
                     /locus_tag="IWL30_001777"
     CDS             complement(4064..5323)
                     /locus_tag="IWL30_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP9706533.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5325..6371)
                     /locus_tag="IWL30_001778"
     CDS             complement(5325..6371)
                     /locus_tag="IWL30_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706534.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFTGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6606..6881)
                     /locus_tag="IWL30_001779"
     CDS             complement(6606..6881)
                     /locus_tag="IWL30_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAP9706535.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7124..8434)
                     /locus_tag="IWL30_001780"
     CDS             complement(7124..8434)
                     /locus_tag="IWL30_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP9706536.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8616..9845)
                     /gene="rpsA"
                     /locus_tag="IWL30_001781"
     CDS             complement(8616..9845)
                     /gene="rpsA"
                     /locus_tag="IWL30_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP9706537.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9980..10660)
                     /locus_tag="IWL30_001782"
     CDS             complement(9980..10660)
                     /locus_tag="IWL30_001782"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAP9706538.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10709..11344)
                     /locus_tag="IWL30_001783"
     CDS             complement(10709..11344)
                     /locus_tag="IWL30_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9706539.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11410..12813)
                     /locus_tag="IWL30_001784"
     CDS             complement(11410..12813)
                     /locus_tag="IWL30_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAP9706540.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12800..13831)
                     /locus_tag="IWL30_001785"
     CDS             complement(12800..13831)
                     /locus_tag="IWL30_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706541.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13862..14083
                     /locus_tag="IWL30_001786"
     CDS             13862..14083
                     /locus_tag="IWL30_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAP9706542.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14248..14985)
                     /locus_tag="IWL30_001787"
     CDS             complement(14248..14985)
                     /locus_tag="IWL30_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAP9706543.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15138..16478)
                     /locus_tag="IWL30_001788"
     CDS             complement(15138..16478)
                     /locus_tag="IWL30_001788"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAP9706544.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16715..18238)
                     /locus_tag="IWL30_001789"
     CDS             complement(16715..18238)
                     /locus_tag="IWL30_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAP9706545.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18616..19227)
                     /locus_tag="IWL30_001790"
     CDS             complement(18616..19227)
                     /locus_tag="IWL30_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9706546.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19350..19475)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19557..20273)
                     /locus_tag="IWL30_001791"
     CDS             complement(19557..20273)
                     /locus_tag="IWL30_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP9706547.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20279..20848)
                     /gene="scpB"
                     /locus_tag="IWL30_001792"
     CDS             complement(20279..20848)
                     /gene="scpB"
                     /locus_tag="IWL30_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAP9706548.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20832..21629)
                     /locus_tag="IWL30_001793"
     CDS             complement(20832..21629)
                     /locus_tag="IWL30_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAP9706549.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21599..21970)
                     /locus_tag="IWL30_001794"
     CDS             complement(21599..21970)
                     /locus_tag="IWL30_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP9706550.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21989..22876)
                     /gene="xerD"
                     /locus_tag="IWL30_001795"
     CDS             complement(21989..22876)
                     /gene="xerD"
                     /locus_tag="IWL30_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAP9706551.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6447 a         4912 c         3900 g         7733 t
ORIGIN      
        1 aaaaatcttg tcacagtccc ttattctgaa taaacggtgt ggaagagaca gcctatcagt
       61 agtaataaga cacatctctt tcacagcctc atttttgcgt aaaaatcgat ttattcgaaa
      121 aattccccgt atacatcttc tacacgttga tctggattga tcacgatata caagttacct
      181 gttttttcgc tgatttttgt tgattgatat tgattgtcta atccttctgt tcctttatct
      241 agatatggga agtacacaag atcaggtgtg ccatttcggt aaaggatttc cattcgctcg
      301 atatcttcgt atttcaatgc gcgattaaac atgcccattt ccagtccgcc taggttgata
      361 tcatgtgtcg caactgcgtc cccttcatgg aagatttcga ttttaaatcc agcacacgga
      421 tggatttcaa caaattcact gccattgatt cgtccaaaac ttgttgtgat ttgtttgatc
      481 caaagatcgc cgatatgacg gcggttgatc gtccaggttt ctccatttct aaaataaaat
      541 tttagcgctg tcatttcttt actcattaaa aagactcctt tatgatgtaa tttattcttg
      601 tactacctca gtttagcaca aatttatgta agtggtcaca tcttatgttt tttcttctaa
      661 tgacgtatct gcccacttta agtagccaac aaagctgagc agatacagtc tagtgttttt
      721 taaatcaatt tttctttcga taaaaaatcc aaaacctgtt ttttatcatt ttttattctt
      781 tgttctacga cggctttttc agctaattgc aaagcttcac taacccactt accaggtttt
      841 tttccagttt cttctagcaa gattttacca tctactgcta attcgcttaa tgacttgata
      901 ggtaaacgat cataggattc tcctacggca tccatctgag gtgtttcgtc aaaatagatc
      961 atcaattctt ctacttgcag tgcatgtttt ttgcctaaga agtacaattc gaatggttcc
     1021 aaagctccag tttctcgttg tcgtagtcct tggacaagtg cttgaacgtc tttaatcatt
     1081 tggttagaac acttccacga tttcaaaaag gaacgaatct cattttcttt tagatccagt
     1141 tggctgatca ataatgccca tgcttggctc tcttctttca atgggacgga tggtaattca
     1201 gcaaaacgta aaagtgcttc gccttcttct ttcaaaccgg gacagcaaat gtagcactct
     1261 gtttcaataa atgcatggat tcccgcggcc cgttcatttc ccaagagcat cttgatgaat
     1321 tccacattta tacgttctac agatatttta cctaatagct cgtggttttc tttgatggcg
     1381 tagaaggttt cttcctctaa tttgaaccct agttgactga caaaacgcaa cccgcgcatc
     1441 attcgtaagg catcttcatg gaaccgctca tgaggattgc ctacagctcg caagatctgt
     1501 tcttctagat cttgcagacc atcgaacaaa tcgatgattt ctccatcttc tttcaaagca
     1561 aacgcattga tcgtaaaatc tcttcttttc aaatcttctg ccaacgaacg aacgaattcg
     1621 acatgatctg gtctacggta gtcctgataa gttgattccg tccgaaatgt ggtgatctca
     1681 tattgttcat cattttctaa taccaacaca gttccatgtt cgatcccaat atctatcgtt
     1741 tttggaaaaa gctgtttgat ttcttctgga aatgcgcttg ttgcgatatc cacatcatgg
     1801 attggctggt tcagcaagat atcacgaaca cttccaccta caaaataagc ttcatatcct
     1861 gcattctcta attttttcag aactggaatt gccttttggt attccagagg taattgtttt
     1921 aatttcattt ttccaacctt tcctgcgtgt ttcttgtcct gagataaaga ctcagttgca
     1981 tggtgactgt ttgacaagtc cgtttcagag tcttcaaggc caggaccact tcatcttgtt
     2041 tactcgtcct tacgctcaaa gcgattatgg tctctttgat tgaatttttc catgtttttt
     2101 tcaaaaactt cagttaagtc gatattcaat gaattagcca tgatcatcgt aacgaataaa
     2161 acatcaccaa gttcttctgc taccgagtta actggttcag aagccttttt gcttttttct
     2221 ccataagtgt ggttgacttc tcttgccaat tcacctactt cttcagttaa tcgagccatc
     2281 tgagctaatg gagagaaata ccccaccttg aattgctgga tatattcatc cacttcctgc
     2341 tgcatactgt ataaagaacg ctttttatcc atctttttcc ctccaaaact atcttaccaa
     2401 aactttaacg caaatcctag ataagagcaa ttgagaaaat cgaatttcca tgctacattt
     2461 aggaaggaat ttctaaatgg aaattctgtg aaaatacata attgaaagct ggaattcata
     2521 tggtcaaacg aaaaacagta aaagaagttc tttttatcat tataggaaca agtatttatg
     2581 catttggtct tgtttacttg aacatcgcta atcatttagc agaaggggga gtctcaggga
     2641 tcaccttgat cttaagagct ttatttggta ttgatccagc ttatacgact ttattaatca
     2701 acatcccatt gattttacta ggaggaaaga tcctcggtaa acgttccttg gcctatactg
     2761 ttctaggtac actttctttg tcattctttt tatggatctg gcaaagggtt ccactagaaa
     2821 tcaatttaca gcatgatcta ctgatcgttt ctctacttgc gggtttagtt gcaggtgtag
     2881 gaagcgggat cgtctaccgc atgggtggaa caacaggcgg cagtgatatt atcgctcgga
     2941 ttttagaaca aaattacggc atcactatgg ggcgttccct attggcattt gatatcatcg
     3001 tcttacttgc ttcattaacc tatatcgact tgaaacgtat gatgtatacg ttgattgttt
     3061 cgtatgtttt cagtcgtgtg attgattcga tcttagatgg cggatattct gcaaaaggtc
     3121 tacttgtcgt ctccaacaaa agcgaagaga ttgcaccact actgatgacc ggtctgcaaa
     3181 gaggtgtgac tttcctttct ggagaaggcg cattctctgg taattcaaaa aaaatcatct
     3241 atatggtcgt tagctctagt gaattaactg aagctaagag gatcgtccac gagattgacg
     3301 aaaaagcttt cctttcaatc atcaatgtgc atgaagtgga agggcaaggc tttacttatt
     3361 tgaagccaca gacaagatta ctgaaaaaac tataagaaaa aagacaaaga taggctatga
     3421 ggaaagattc cacatccagc tgacctttgt cttttttagt actatactat ttttaactaa
     3481 ttgttatgtt cacttacccg gttacttttc ttttgtgtct gcaaaaggat ctttcggtta
     3541 acttcatcag ataatttcaa aaaagcatcc ctatctccat cttctagtgc ttgatcgatt
     3601 tgagcataaa gcatttttag ttttgcctct tcttcttttt cttgaaaata tcgttcaatt
     3661 aattcagtat cttcttcact gactgaagaa tcccaagaag aaaatggatt gtcttctaag
     3721 atagatagat attctcttgt ctgccaagca ttttcgaata tacattccac atataaaggc
     3781 tttttccaat tcaaacggat ttcatgaaaa atctggtctg tgtccgtaaa ttcttttccc
     3841 tgcaaaaaca atttcatggg ttccccttca acagatagat cactgatctg cagtcctcga
     3901 tccgcaaaat tcgccccttc aacaaagtgg acattgctta aaatcgcttc atgattggca
     3961 agataattca agatccaaaa aacttctctt cggctaaaag atacatgatt gaccatccac
     4021 gttaaaaaat tcttcttttc ccgtacatca attagcattt ttctcacctc tctgttaggt
     4081 catctaataa tgacagtgcc tccatatctc ctggttcaag tgttaaataa taagtcaata
     4141 attcttgcgc tcttttcagc tgcccttctt ctcttaagaa aaaggcatac tctttcaaga
     4201 aatctggttc atgtgatagt tcatgataag cctgttcata gtgtacagct gcaacggtaa
     4261 agtcttctaa ttcattataa gcatgggcta ggttccattc tgcataagga ttagcagttt
     4321 cttccatctg attgatcact tgtatcacat cttcatatct ttcatcatcc aagtaaagat
     4381 tacttaaagt aagtaatgtt tcatcttgtt tgtctcctac ttctaaagct ttcagtaaaa
     4441 gttcttctgc tcgctgtcta tcatgcagac ggaaagcatt ttccgaggcc aggtgataaa
     4501 gttcgacttg gtaaggattt tctttgattc cttcttctaa cactgtttgt gcttcttcaa
     4561 tcatttcttc ttcttgaaga gcttgtccta gatatagata taacgattga taatgcggat
     4621 taacttctcg aagttcttcc agatagttga ttgcctgctg attttctttc aattgaagat
     4681 tcgtaaaagc tagctggaac agtcgatcat ctgtcctttc ttctttcagt gcttcttcta
     4741 aaggtacgat agctgcttcg aattctcctt gcatacttaa ggattgtcct aaacgttcct
     4801 gcatcgagat tcctgaaatt tcatttatcc cgcttgccaa caatgtttca taaaccactg
     4861 ctgcctcttt gaaacgatca actgaaaaat acaattcagc tagcgcaaac tggatcaatg
     4921 gttcatcagg aagcaaccca gctgcttctt tcaattttgc ttcactcact tcagggattc
     4981 cgataacttg gtacaaatca gctatcgcca ataagctttg cggatagtaa tcactttgct
     5041 tatccacttt ttccaggtat tcaaaagcta agtcgatctc attatcttcg attgcaattt
     5101 ctgctaacgg gatgttcagt ccgtcatttt ctgggtattg gttgatcagt tgttcaaaaa
     5161 tctgttttgc ttcgatcaaa aagccaatag ccagcaattc ttcgcctagt tcagctaata
     5221 tatctgctgg gtcttctttt aatgcttttt ctaaaaagat ttgtgcttgt gacaagtctt
     5281 catctgataa agcttgcaac attttttcgc tatttgtatt catttcaatt ccctcttatc
     5341 tcactgacga tttgttgaaa taccgtcatt gttctcaata attctttttc gtttggatgt
     5401 tggtatccgt ctattatacc tattttttct aaaacaagta tttttttgct tttttctgcc
     5461 aaaaaaagaa ctttttccaa atactccatc tttgaaatct gttccagcca gtggattgga
     5521 tagccaagca agtccagcca cacaaaaaag ttttttagtt ggaaatcaaa aggatatgct
     5581 tggagattcc acacgaaatg aaacatcatt cctaaaaatt ttttcatact agcagaaagt
     5641 agatggccat tttcggtcag ataaaaagct tgttcaaaca gtttaccata atttgacagg
     5701 ttttctgcct ctaattgata aaaatagatc atttcttcga tcatcccggt aaacggccga
     5761 gtcgttaatt gctttttatt tggatagttt ttatacagat tttgcaaaaa acgatgatca
     5821 caaatcattc cgcaacagat aaaagtcaaa aagtcaatct gctttccgtc cacctgccgc
     5881 tcgctgattg tttgatcata aacgatgcgt tctggcagat tgatggtttg cagcactaca
     5941 cgatttggta gctgcaaaat cgtccgctgc ggtgttaatg ctcggcttag agaacggatc
     6001 gatacaggga tcatccatag tgatgaatca agggttgtat gtttttgaaa aaaacctgcc
     6061 agatccatga tcccttcatt tccaaatccg atgataaaca tggattgatt ttcgggatag
     6121 cgtttgacga aatcaagcag gttctttaat tctgttaaat gattgcattg agtgttggca
     6181 caaatatacc agtcattgtt tgcttgattt gcaaataacg ggttgatctt atctgcaaac
     6241 aaatcataat agcgctgatt cgttaaaaat aagattggct gatcaatgga aagttctctt
     6301 agctgactga ctagcgtttc tccgtaaaac actttcgtca acgcatcttc tacttgataa
     6361 tcaagttcca tacgcatccc tcctcttctt atttttattt taccattagt aagcaaattc
     6421 tagaaaagat aagagcaaag ctcacctcat aacttggaaa taaataaaaa ttaaataaaa
     6481 tacatgtgtt ttctatgtat gacatacgga ttcggaatct ctgtctctta tttattcctt
     6541 ttctgctagg aaataaaaag aaaagcactt gtagccctag agccacaagc gctttttcaa
     6601 ctgtcttatt taacagcgtc ttttaatgct ttacctggtt tgaatgcagg tactttgcta
     6661 gctggaattt cgatttcttc accagtttgt gggttacgac ctttgcgagc tgcgcgttcg
     6721 cgaacttcaa agttaccaaa accgattaat tgaacttttt cgccgttagc taaagcatct
     6781 tgaatagttg aaaatacagc atctactgct gcagttgcat cttttttagt taaatcagta
     6841 gctgaagcaa ctttttcgat taattctgct ttgtttgcca tggatagatt cacctcctcc
     6901 acaagagata tgatagaaat atcatttagt taaaactttc tgagtggtcc agaagtttaa
     6961 aaagaaatat tgattaactt caatattctg ttatcacgat atcatatgaa ccgcttaata
     7021 gcaaggtttt taccaatttt cacatgcttt ttttagtcat agaaacgtaa caaacgaaac
     7081 tgtctttttc ttttgaaaaa aaaacgtcga tatggtaaaa tgcctatttt ctcctacgag
     7141 ggataattcg gatggacgtc ccttcaaaag taaatgcttt tctgatttga ttttctaaga
     7201 aacgttcata agaaaaatgc atcaattcct cttcattgac aaaaacgaca aatgtaggtg
     7261 gtttgacagc gacttgtgta gcataaaaaa tcttcaggcg ttttccttta tctgttggtg
     7321 ttggattgat tgctacagca tccataatga tatcattcaa cacagccgaa gagatacgaa
     7381 gattctggtt catacttact tgttcgatca tttcaggcag acgttccaac cgttgtttag
     7441 tcactgcaga aacaaaaacg attggagcgt aatcaagata ccggaattca tcacgaatct
     7501 cttgttcgaa atcacgcatt gtattggttt ctttctttac aagatcccat ttattcacga
     7561 cgataataat tccacgacca gcttcatggg cgtaacccgc tactcgcttg tcttgttccc
     7621 gaatcccttc ttcagcattc agtaccatca aaacgatatc agaacggtca atggcacgca
     7681 ttgcacgcat cacgctgtat ttttcagtat tttcgtatac ttttccgcgt ttacgcatac
     7741 cagctgtatc tatcattaag aatttctgac cactttcaga aacgaagtgg gtgtcgattg
     7801 cgtctcgggt cgttccttca atatccgaaa caattacacg ttcttctcct aaaatcgcat
     7861 tgatcaaaga ggatttacca acatttgggc gaccaatcaa actaaactta atcgtgtcgt
     7921 cgtcctcttc ttcagtctct tcagagaaat acttcactgc ttcatctaaa atatcaccaa
     7981 tccctagtcc atggcttcct gaaacaggat aaggatcgcc tagccctaag gaataaaatt
     8041 catagatatc gtttctcatt tctggattat ctactttatt gactgctaaa atcactggtt
     8101 tattacttct gtataaaatt ttcgcaacca tctcatctgc gtctgtcacg ccttcgcgtc
     8161 cgctgactac acaaatgatt acatctgctt cttcaatcgc gatttctgct tgatgcttga
     8221 tctgatccat aaaaggttca tcgccaagat caattccgcc tgtgtcgatc acgctgaatt
     8281 ctcgacctaa ccattcacct tttgcataaa tacgatcacg agttactcca ggagtatctt
     8341 cgacaatcga gatacgctca ccagcgatgc ggttaaaaat cgtagactta ccgacgttcg
     8401 ggcggccaac gattgcaatt gttggatttg ccatattatt tcctcctcac aagtttccaa
     8461 tctcacttag tttaacgaat gcattgccaa ctttcaagca tagattgtca attaaacgaa
     8521 gtatttttat tccttattga aatactaatc gcttttcaaa agaaaaactg agacaaaagc
     8581 agctgctttc atctcagtca cagatacaaa atcaattatt tttcagaatc atcggttgct
     8641 ttttcttcta cttcttcttt caacgcatca cctaaaatgt ctcccatagt aaatccagta
     8701 ttttcttctg gtaattcata agactcaaca ttttttggtt cttcttctga agctggtttt
     8761 tcttctaaag ctttgatact caatgcaata cgatgttctt ctggatggat ctctaatact
     8821 ttcactttca cttcgtcacc ttcatgaagc acttcatgtg gtgtcgcaat atgtttatgt
     8881 gaaatttgag agatatggac tagcccttct actccaggga acacttcaac aaaggcaccg
     8941 aagcttgtca aacgtttaac tgttccgtct aatactgtgc caactggagc ttttgtatcg
     9001 atttctgacc aaggtccagg ctgtgtgtct ttgattgaca aggaaacacg ttcttgttca
     9061 ggattgatag acaacacttt gacatttaca tcatcattga cagccaatac atcacttggt
     9121 tttgctacat gactatgtga aatctctgag acatggacta gaccgtcaat tccgcctagg
     9181 tcgacaaatg cgccaaaatc agtaagacgt gcaacacgtc cactgagaac atctccctca
     9241 tggatagttg aaagaatttc ttttttcttt tcttctcttt cgttttccac taccgcttta
     9301 tgcgataaga tcaaacgatt ttctgatggt tcgatttcga tgattttaaa gctcaatgtt
     9361 ttgcctttat agtctgaaaa gtctgcaaca aaatgatctt ccaccattga tgcagggaca
     9421 aaaccacgta caccgacgtc aacgactaaa ccgcctttga caacatttgt aacaggtgct
     9481 tcgatgacct tgccctcttg gaaatctctt tcgatatctt cccaaacttt tttagcatct
     9541 aaacggcgtt ttgaaagtag gtagctgccg ttttctttat ctttgccgat cgtactgata
     9601 acgactaaat ctaatacatc accgactttt actacttcgc tgatatcttc taccggcaaa
     9661 gttgataatt cttttgccgg aacgactcct tcaactccag ccccttcgat tccgacaatc
     9721 acttgtttgt cttcaatgac taaaacttcg cccttaacaa catcgccaac ttttacttct
     9781 tgtacgcttt tcattgcgtc ttccattgac tggttgttct cttgattttg atcaaattct
     9841 gtcataacac ttgtcctcct aaccaacatt ctgccgtaag tacggtactg tttttagttt
     9901 aattgattat tcaaaaaaaa taaagcgatt tcgttcgttt tccttgaaaa aacaattgtt
     9961 tttcattttt tttcttaatt taaaaaagaa gataaccttt tttcaaaaca acagctttta
    10021 ttgctgcaac gacttctgga atcgatttgc cagtcgtatc gatacgaaca gcatcctctg
    10081 cttgcttcag cggagagacg tcccttgttg aatcataatg gtctctttcc tgtatctctt
    10141 tcgtcagtgt ttcaagatca gtcggaattc ctttttcttg gttctctttg aaacgtcttt
    10201 ctgcacgctc acttacgctt gctactaaaa agattttcac ttctgcattc ggtaatacag
    10261 ctgtcccgat gtctcttccg tccatgacaa cgccaccgcg ttcccctatt tttcgttgga
    10321 cagcaactaa ttcttctctg acttttccat gtgcagagac ttcagaaaca gcttttgtga
    10381 catctggttg acggatttct aaagtcacat cttttgatgc tatgaataca tgctgtccat
    10441 cttcttgttg cgaaaaagag ataggatact ttttgatcag cgaaactagc ccttcttcat
    10501 ctgaaaaaga tacattgttc gtaatagcta aataagttac tgcgcgatac atggcaccgg
    10561 tatctgtata aatataattc aattcttttg ctaatatctt ggcaacggta cttttcccag
    10621 atgaagcagg cccgtcgatt gcaatactga tttgtgtcat gatataactc ctttttctca
    10681 gaaaaaaagc cgcaaagcgc ggctattatt atttaattct taattcttga ccagggtaaa
    10741 gcataaagtt gtttggatct aagccattca attcaaacaa ttgatcagtg gtgatccctg
    10801 cacgctcagc cacttgtttt ggaccttcgc ctgcttgaac agtcgttgtt gaaccggctg
    10861 cagctgatga agaaggtgtt gtttctgctg cattgctgct tgaatcccct gcttgataag
    10921 ttgcctcact tgaggaagtc ggtgttgtct ctgctgcaat actgctttct gacgttacac
    10981 ctgtatcttg agacacagta gattcttccg cgattgtact agattctact gttgaagtct
    11041 cagttgactc ctcttttgtt gtacttgtag aactttctac tactgatgtc gaagtttcag
    11101 aagaagttgc tgctgttgca ttgttgcttg atccatttct gatccagaaa taagcacctg
    11161 ctggaatagc gatacataag accagtaaaa tcaaaaatag cgaaagaaat aatgtatttc
    11221 ctttcttttg ttgtctctga gaacttcttg atgaaacttc atcatctgta tcgtagattg
    11281 gttgttccca tggttcttgt gtttcgttat tttctgtaga ttgatgtcta tcttttctgc
    11341 tcactgtttt acctcctaaa ctctatcgtg tgtattatac cataggaaaa agtgtcttgt
    11401 cctaaaaaat caatgttcca tgtttttttt aaataaattt agtaaaatcg tttcccaatg
    11461 aagttgttca gtcttagaaa aaagcttcct ctttggcaga agttctgctt ttgctccatc
    11521 aatatcacaa caattctgcg gcttttcgga gagtttttcg ccaaaataag ctaagatgaa
    11581 ttttctgcgg cagttttctt cattaatata gcgaagcatc tgctgcaggc ggaattcttt
    11641 ttctttttcc tgacgtttga gccgttccac ccaattttct ggtttttcag actgctggat
    11701 cagctccatc catttttttt gcagctcatc aaagggagct tgctttgtgg catattccaa
    11761 ataaagctca aaactttgtc gctgttctct agaaagttga ttaaagaaat aatggatacg
    11821 ttcatcccca ctttgataaa aaagaattgc tgcactttct tcctgatctc tgcctgctcg
    11881 accgatttct tgtacatagt tttctagact gtctggcaaa tcataatgga ccacataacg
    11941 gatatcagat ttatcgatcc ccataccaaa tgcattcgta gcaatgagaa actgcagctg
    12001 attttttaca aactgctgtt gaagttgtct tctttgagct gcatcaagac caccgtgata
    12061 gtatccaact gtaaaacgtt ccctgaataa atgatacaat cgttcgactt ctttttttgt
    12121 tgcacaataa atgattgccg ctccttgtgc tctttccatg aagtgttcaa gttcttgttc
    12181 tttttgctgc gttttccgaa caaaaagcga gatattttgc cgattgactg attgtctgat
    12241 ctcttttggt ggtcgatcaa atagtacatg acgtatatct cttgctacta aatctgttgc
    12301 tgtcgccgtt agtgccagtg tgaccgggtt tcctaaaagc tttcggattt ttcctaactg
    12361 ttgatattct ggacggaaat caactcccca ttgagacacg caatgagcct catctactac
    12421 atataacgca atattttgtt ttttcagatg ctctaaaaga gaaggattag tcaacatttc
    12481 gggactcaaa aacaaaaatt tatattgcga tagatgacgg aggacgtatc ttctttcaga
    12541 ttgcgaaagt aaactattga aagcagctac tcgtttctcc cctcgttttt gtaattgcgt
    12601 cacttgatct tccattagag aaagcagcgg tgacacgatg atgaccagac cttccattaa
    12661 ataccccgtc aattgatagc aaaggctttt tcccgttcct gtaggcaaaa tagcgagagt
    12721 atcttctcca tcaagcaacg cctgaataat ttcttcttgg ccgggacgaa atgaatcaaa
    12781 tccaaaatat ttttttaatt catttattaa cattctgtat cgcttccttt ctcaatagat
    12841 agatttgata caaacgaaat tctccataat ctaattgtcc atctacattc cattcgctaa
    12901 agcgccactc tcgcacggaa tccttatttg gttccaaacg ttcgattgtt tctgcagaga
    12961 gaatccactc gtatggaaaa tcatcaaaaa acaaggccca ctcaataaga tgatctgtta
    13021 ctgttcctct ttttaaatga cggattgcca tgatctggtc aattgtctga ccattcataa
    13081 acatctgctt ggtgatcatc atgctttgat tgaaattttg ctgtaataat gaatcgatca
    13141 aagcgtgcca aagcgaatct ggccgttttt cgatttgtgc taacaaaagg tggatacatc
    13201 tgctttgttg cagataaacc tctgactcat cattgttttt tgccagctga taaggaagca
    13261 gccctgttcg ctcatttcca gataattggt tagctaaaaa atctgctgct tcttctggta
    13321 tctccgaaaa cagctgcacc aaactctcat acgcagtatc aattaaaatt tcacgtggca
    13381 attggctttc actaagccat ttttttagtt ggaaagtata aaaaggactt gtctctgctg
    13441 gaagatagtc ttgaataccc gttgccagat tagaaattac ttgaacagca aatttaatga
    13501 gccgccaact agtttcccct gttctgccat agcggtcaaa acgcaaacca gtatgttcta
    13561 tttgatgttc ggacaacata tcagcccctg caggcgttag ctttgcttca ttttcattta
    13621 tttcgatcca accttgctgc gcaatttttt gcaaaatttg ataaaactta tcctgcttca
    13681 attctggaaa actgttatgg ataaacaaaa gttcgtgggt aaatccataa ataagtactg
    13741 aagtcgttcg ttttcccaca aggagttggt aaagagaact aatccgcagc ttgtgtcctg
    13801 atgaaaatag cgataaaata aaaacaatca tattattctc cttaagttag gatggttaga
    13861 aatgtcacta ctatgtaaac ttgtacctga acgctgtatt gcatgcggtc tttgtcaaat
    13921 cgaagcgcca gatattttcg attatgatga agaaggaatc gttttatttg cccaagaacc
    13981 agaggctcat caacagttcg tgatgccgga agaagaaaca tatgtaaaaa aagcgtatca
    14041 aaaatgtcca gttcgagcga ttttattaga aaataaaata taaatataaa cgaaacttta
    14101 aaaacgtaca ctaagttctc taaacttgaa aataagccgg gatctgtttc tcttgttccg
    14161 tgaaatcccg acttattttg aaagattcat ttgctatcta tagttaattg actagttttt
    14221 tcacggaccc tataggaccc atttatatta tttcaacttt ttcagccagt tttccaatgt
    14281 attgacgatc acagtttgct ggtctgcgtt agaaatttct gcctctccat ctcctttttg
    14341 ctcaccatag ctgccaaaac ctccatgatt tccgccattg attgatacat actctgttct
    14401 ttccggtaaa tactgcttag cttcttgata agcttcctga ttcaatactt tatcttttgt
    14461 cgccgtgatc gataaaacag gaacagctga ttctgtcaat gttccttttt tgtcgggata
    14521 acttgccatg aagaataccc cttttaaatg tttgtctgaa tgattcttat tgacataacg
    14581 acttgccatt acgccaccta atgaatggcc accgatcaca taatcggaat ttggaaaatc
    14641 ttctttgacc ttatccgctt tgtttccgcc catcactgct agatcaagtg gcattttgac
    14701 aatagcgact gaaaagccgt tttttgctaa atcttgcgcc caaatactat aactttctgg
    14761 ctctacgaga gcaccaggat aaaaaataag taatggagac tttccatgat tctttttctc
    14821 tccttcaaaa aataagtaat cttctttgtt ttttccttct tttaccaaca cttgcgcttc
    14881 tgacgatggt tcgtacgtat tttgctgtat atagtaataa ccggctccac cgagtaatcc
    14941 taaaatgatg actaagccaa ttagtattcg tctaaatatt ttcactttct cccacctttt
    15001 ctatctgagt gacccctttt tcttaatcaa gtatgattat accttaaaaa aataagagag
    15061 aaatgcaaac tttccagcat caaataaaaa aagacggaat atccatttga atatccgccc
    15121 tttttgtatg tttattttta tttagcacat aaacgataca cagcttctgc ataaatttcc
    15181 attgctttat acatactact taattcccat cgttcattcg cctggtgcat aaagtcagga
    15241 gtatcaggga acatcgcacc gaatgccaca cactggttca tcgtacgagc aaaagtagcg
    15301 ccgcctgata caaacggttc agtcatgtct ccagtaatat cgcgatatgt ccccaataga
    15361 tttttcacta actcactgtc tagtggcacg tataatgaat caaggaagtc aaattcttca
    15421 taagtcaacc catatttttc tgctgtttta gttaatttag ctactaaatc atctttcttg
    15481 aatgtaacag ggattctcat atctacaccg atttttgttt gttctggtgt gatttccaaa
    15541 cttgcaaagt tcatcgttag ctcgccggat tgttcatctt ctgttttgcc cacgacagct
    15601 tcaccagttg cattttcttg aactaatttt cctaagaaat tgattgggcc aaaatcaaat
    15661 acttctgaaa gagcaattgc cagacgagaa atagcattca ctccttgtgc tgcatctttc
    15721 gcgtggatac ttttaccaag aacgacgatg ccttcacctt gttcttcgaa atcgaattcg
    15781 tgttttttca atgcttcttt tacttcagaa agcttttctc cagagtaagg agctgcatca
    15841 ggaacaacat tcaacgctcc tccagctttt acagaaaact cgttcgttcc tggtcctgtc
    15901 aagtaagctt gcaataagcc tttttctgca taaattaaag gaaattcagc atctggcgca
    15961 aatccttgcg taattccttc ttctttttcg ttatattttt ctaaacaacg ccataaattt
    16021 tcttcatctg taccaaagat aaaacgaata cgtgtcttga attccacgcc agcatcaatc
    16081 aaagctttga cggcatacat tgccgcaata gaaggacctt tgtcatcttg ggatccgcga
    16141 ccaactaacc agccgtcttt tatagttgga tcgaatggtt ttgtttccca attattttcg
    16201 tcccctgctg gaacaacgtc catatgacac aagataccga ataattcatc tccagagcca
    16261 atctctgaat aaccgtaata gccttctgga tctttaaatg ttctaaatcc tagcttttca
    16321 ctgatttcca atactttatc caaagcttca atcacttttt taccgaaggg atgtccttgt
    16381 cctgaatctg cttcatctag tacggaagga attcggatca attcgctcaa tgattctaat
    16441 gcttgttcat gatgttgttc tgtcacaaat tttttcatga tattatcctc ctaatatgtt
    16501 aaaaagggtc atgtcaaatt cctccgacac aacctcttct tctcaaataa tgacgtttta
    16561 agcctgctcc atcagcaagc agaatgttct taacaaatga tgaaagaaaa aacactcact
    16621 gaatggagct attgacttgt ccacgtgctt atcgatagcc gtttatcggt ttcttcattc
    16681 ttatgataga ccttgtacct gtatttgtct aggtttagaa cattgctgct acacctagga
    16741 tcacacagat ggctacaaat aatatcgcaa ttaatttacc ggtaaatttc caccacactg
    16801 aaacatcgaa acgagcaatt gctacagcac ccattacgat agcagatgtt ggtgtaatta
    16861 agtttactaa accagatgca gattgataag cagtgattac taaatcctta ccgacgccgg
    16921 caaattcacc cataggtccc atgattccca ttgttgcagc agctaagccg gaagttgaag
    16981 gaatcaagaa tgacataggg atatagaaaa taaatgtaat aatgatgaat acacttgaag
    17041 ataatgaact taatccttgt tcgccccaat gtaagatggt gtctgtgatt aatccattgt
    17101 tcataaccac ttggatacca cgagcaacag ctactacgat tgctacaccg atcaaatcag
    17161 aagcaccagc taaaaattca gagacaaatt cagactcttt catgccataa gtgattgcta
    17221 ctacgatgga cattactaag aataacatag taatttctgg gaagtaccag tcacctaatg
    17281 gaagcatgct tttacctaga accgtaccta gtaccggaag gtttgttaac cagtttgtca
    17341 atgattcaaa aatcgtgaaa ttcgcattca aggttgtcca aggaatcaaa ctgatgatca
    17401 tgataccaaa agtaataaag aacaatacat taacatgttt ttgttttttc gtgattactt
    17461 cttgacgacc gattgcttca atatcgaaat gcttcatatc ttctttacgt tggttgtata
    17521 ctaatgattt tgttggatct ttttcgatct ttgatgcata acgatacaca tatacaatag
    17581 aaatagctaa caaaataacg aacatcaaga atcgaagacc aataccttca cccatcgaaa
    17641 tcccaacagc ttgtgaagct acgcctgttg caaaaggatt taccgttgaa gctagacagc
    17701 ctacttgaga ccctactaat acgattgcta cagctactac tgtatcaaag cctacagcca
    17761 tcatgactgg gatcagtaaa gcatagaatg gaatcgtttc ttctgccatt ccgtaagtag
    17821 agccgcctaa tgcaaataac agcatcaata ctggaataag cattttttct tttcctttga
    17881 atttcttcac aactgaagca atcccggcat ctagtgcccc tgttttgttg ataataccta
    17941 aaaatccacc aacaaccaaa atgaagaaag agattgggat tgcagcaggt gtttctttag
    18001 ttagctcccc cgctggtaca cctagcatcc cattgattgg cgccatgaaa atatcccaca
    18061 gcccttgagg attttgttgt actcgttcat atgatccagc gacgatgttg cctgcatcgt
    18121 ctacactata gtgcccagct ggtataaacc aagtaagtac agcgattact gcaataataa
    18181 taaataagac cgtatacgct gaaggcattt gaaatttttt cttttttgtt tgttccatct
    18241 tcttcttcct ttcttttccg ttagagacaa caattttggc tctaatgtta ttttatcaaa
    18301 aagagtatca gaacaaaaat aataacacgc attttgtaaa acttttgttc aaaaagttcg
    18361 tttttcccta ttccctcctc ttttttcagg tttttaaagc tttcttcttt ttcattgtag
    18421 cgtttccttt tttttataaa caaacatttt cgtcttttaa aaggagaaga atttcacatt
    18481 tgcaaagaat tgattgattt ttatgcataa atcaaaaaaa tactcaaaac aaacaaataa
    18541 aacgtttaca ttattgcatg tcttgtattt ttttccaaaa aaaaagacca agaaatttct
    18601 tgatcttttt gatatttatt tgactgctgt atgacgttgt tcgaactgat gttgttttcg
    18661 acttaaccat ggaagaagtt gcgcatgcag cactaaaaag acagcactca cgatgaatcc
    18721 tttgatcaag ttgaaaggaa cgatcccaat cagtacatag tttgctaaag acatgcccag
    18781 catctgtgtt gcgttcagcc ctaaaaacat taagtaaaga ggcgtaataa cgaaataatt
    18841 tgcgatactc ataaaaatag ttagagcgat ggttcctgta atgacaccta aaaatttgtt
    18901 tcttccttta tgctgtttct ttacactaaa gaaataataa atcgggaatg taaagatcgt
    18961 tgttgataag aaactagcag tgtctcctac caaattatcc ggagaaaaac ctgtagtcag
    19021 cagatgtaat actgaacgca agaacgctgt tgcaactccg gccgctggac cgaataagaa
    19081 catgctgatc aataccgaga tatcactaaa atcgatcttc aaaaaactaa aagccggcat
    19141 gatcggaaaa gcaaaatact gtaaaactaa tgcgatcgca gcaaacatcg ctactgcaac
    19201 cattttctgt acacgggtgt tcttcatttg aaatcctcct gttaggactc ctcttcagcg
    19261 aactttttga cgtttctctc caaatgaaaa aactctattt tccagggaaa atagagttag
    19321 agttatttga tcaagcgtcg tcaaataagc tctatcttct ttatccagac tatactgtcg
    19381 gtatcggaat ttcaccgatt cagctatttt tttgcaaaat aggtcgtgga ctataaccac
    19441 cggtcgggaa tttcaccctg cccctgaaga cgaaccttat ataatttttg tttacatatt
    19501 cattatacat atctattcaa aaaacgcaac atctaacact tataaaaagt aagtgttcat
    19561 tttgagtggt tcagcaaact gctgacttct tttttgttca gttcacggta atccccagga
    19621 cgtaggcctt gtaaagtcag atctccgtat ttttcacgtt ttagtttttg cacaggaaga
    19681 ccaactgctt gaagcatatt tttcacttga tgattacgtc cttcgtggat cgtcaattcc
    19741 acgatactgt ggtttgtctt aggatccact gataaaattt ggaatttagc tggagatgtt
    19801 tttcgtcctt cgatctttat tccttttgtc aaaggtgcaa gcatcgattt tgtagcgatc
    19861 ccttttacct ttgcaacgta aactttatcc acttcatgtt ttggatgtgt caggcgctga
    19921 gcaaaatctc catcatttgt cagtagcaag atccccgaag tatcgtaatc cagtcgacct
    19981 acaggataga tacgttcttt taccatcggc aaaagatccg taacaacttt tcttcctttg
    20041 tcatctgcaa cagcagaaat aactccttta ggtttgtaca ataaatagta accgtattct
    20101 tcttgataaa ttgggacttc atccacttcc acagtgtcat gtttcccaac ttttatccct
    20161 aattccctta caacttgtcc gtttactttt acacgtcctg caaggatgaa ttcttctgct
    20221 tttcgacgag aagtgatgcc agcgtgggca attactttct gcaatctctc cattacaatt
    20281 attctccttt attcattcca tcaaagaata gatctggtga cacgtcttct tcaagtgaaa
    20341 tttccatttc ttgtgtatca ggcagttctt ctaatgattt caaaccaaaa taatctaaga
    20401 agtagccagt tgttccatat aatatcggcc gacctggtcc ttctactcta cctttttctt
    20461 caatcagttt atgggcagct agacgctgta ccgctcctga aacctgaact cctcgaatct
    20521 gctcgatttc agctcttgtg attggctgtt tataagcgat gattgctaag gtttcgacgg
    20581 cggcttgaga taatgtgttt gatgtccctt gcgcatatcg cttaagtaag ggagccagct
    20641 cttttttagt cgttaataca taatggcttt ccgtttcaaa aatatgcaaa gctgagtgcg
    20701 agttttgagt gtaagattct tttaattctt tcactaaact tgtgacttct gcttcgctga
    20761 gatttaacag tccgcttaat tcttcattac tgatcccttc ctcgccaaca ataaaaagaa
    20821 gggcttcaag ttcacttaat ttcgtcatgt tcggtatctc ctactgctgg ataaagcata
    20881 attgattcat agttttcttt ttgttgcgca atgatctgcc cttttttcat caattctagc
    20941 aaagccataa aagtagtgac aacttcactt ttagaaggtg actcaaaaaa agaatcgaaa
    21001 gtacagccat gcgcatttct ttttatacgc tcttcgataa ataccattcg ttcttcgatc
    21061 gttgtgtcat cgggcgtgat cgttttttcc actttttgag aaaatttctt acgttctaag
    21121 attttctgca tggcacggat caaatcgatt tttttgactt gtcctcgctt caacggcttt
    21181 tcagcatccg ctaacccatc gatgttcata ggttcttttg tgtaataaag acttcgttcc
    21241 tctgcttttt ctgtcagtct ttcagctgca tatttatatt tacggtattc taaaagctga
    21301 gtgaccaatt cttctcgagg atcttcttcc cataattctt catcgtctgt gccgaattct
    21361 tggacaggaa gcaatgtctt gcttttgatt gccatcaatg tagcagccat cactaagtat
    21421 tctcctgcaa cagctagctc gagtgttttc attgtatgga tataattcat atattgctcc
    21481 gtgatcgtag cgatcggtat atcatatata tcgatttcca tttgctgaat cagatgaagc
    21541 agcagatcga gaggaccctc gaatacatct aatttaatat tgatctccgt caactcattc
    21601 actctttctt ctttttcttt gctagccatt ttccaatgta aggctctgtg atttcagttg
    21661 cacggatgga gacatccggg tacttttcct gtaattcatc taacatttga aagcccatat
    21721 tctcaccgcg ataagacgga ttcaaagaaa tgtcatggat gatcagctga tcgttcgttg
    21781 cccagtaaat accaataatc ccttgaatgt tggttgaacc ttctggcgta aagaaaaaaa
    21841 gttcataatc gtcttcagat tcgtacgtat caatctcctt caacaaagaa gttttacctg
    21901 tcagtttctt atggaaactc aatagtccca tagcaatttt acggttttct ttccggtaat
    21961 gtgttaacat cttctcacct tcctatgact aagcccttgg aaaatgctgc ttataaacat
    22021 ccgtcattct tttcttcgtg atatgtgtat aaatctgagt cgtagagata tccgcgtgac
    22081 ctaacaattc ctgtactgtc cgcaaatcag ctccattttc tagcagatgt gtggcaaagc
    22141 tatgccgcag cgtatgtggt gttacttctt tgtaaatccc agcctctcgt actaactgct
    22201 tcagattttt ccagattcct tgccgagaaa gacctttccc atgattattg acaaatacat
    22261 gcatttcaga tgcgtccttt acaagtaatg gacgtgcttc gtctaagtag cgttccagcc
    22321 attggatcgc ataatcacct aaaggaatga tccgctcttt atctcctttt ccaactgtct
    22381 gtaccagtcc aagagaaaga tgaagatctc ccaatttcaa tccaatcaat tcactgacgc
    22441 gcatcccagt agcatacatg acttccaaga ttgcacgatc tcttatccct aacgtttttg
    22501 tcgtatcagg tgcttcaatc agcttttcta cttctgtcag agtcaatgta cttggtaatt
    22561 tctgtgcttt cttcggcgta tcgatatgtt gcatcggatc atgatcagtg atacgctctt
    22621 gccgtaaaaa ctgatggaat cttctgagac ttgagatcat ccgagtaaca gttgctgtcg
    22681 cttttttttc ttcatgcaac ttctccagaa aacggatgac taaaaagcga tcgacatcct
    22741 gccatgaagc aattttctgc tcctgtagaa aatgcaagta ctgttccaaa tcacgttcat
    22801 agctttgtcg tgtattggcg gacaatcccc gttcaatatt caagtagtgg agataatcgt
    22861 tgatttgctc tttcataata atccccctca tgcctctcta tcatatcaga taccgcttca
    22921 ttctgatagt ctttttccat aatctgagca aagaaaaagc cgtctagaca ctcagacgga
    22981 ctggtttctt tt
//
LOCUS       DACDUU010000047        22977 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.047, whole genome shotgun sequence.
ACCESSION   DACDUU010000047 DACDUU010000000
VERSION     DACDUU010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.047"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..876
                     /locus_tag="IWL30_001796"
     CDS             <1..876
                     /locus_tag="IWL30_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9706552.1"
                     /translation="ILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQ
                     PGYVQYGAGSYANANSPVQIELQHTHDKVTFEKNYKAYVELARDSANKYGIPLTLDTP
                     YNQPGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPV
                     IDPTRAGAANPTLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKE
                     LARVRADGIYRPDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGA
                     QDFHDKRWYLNIPKR"
     gene            991..2199
                     /locus_tag="IWL30_001797"
     CDS             991..2199
                     /locus_tag="IWL30_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706553.1"
                     /translation="MTAEIGIMNKSGIVLASDSASTIGDSKVYNTAKKLFTLDSMHSV
                     GIMIYGNAEFNGIPWEIIITQYKKSIGSSVFNTLEEYADNFIEFVKTASFIRSEQTEQ
                     EQMIGVFQKIISGLFESIEVDINFLISQGTQIDKDVLVKLLQTKMNTNLSQQSQTFIL
                     DIEKALFLSNYGEILKDILNSISTMEGVSEAISEEIQSYVYEIIIRDDVYSSPTGIVI
                     AGYGRMDIFPKLYSYNMFGFVMNILKYSEYESAQIGNDNGSLRSTILPFAQSDVVNTV
                     VQGVDPQITNYLSSQVDSFDDNGKNTYINIIKNISEFQQNQFIFPLLNMIALLPVEET
                     AIIAETLLNLTSFKRKYTTSVETVGGPIDVLAITPNDGPIWIKRKHYFDIDNNIGYRL
                     RKERANDYNN"
     gene            2608..2681
                     /locus_tag="IWL30_001798"
     tRNA            2608..2681
                     /locus_tag="IWL30_001798"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2642..2644,aa:Ile,seq:cat)
     gene            complement(3194..3493)
                     /locus_tag="IWL30_001799"
     CDS             complement(3194..3493)
                     /locus_tag="IWL30_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706554.1"
                     /translation="MLFNETQLWFKFDPSNRFIKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(3499..3729)
                     /locus_tag="IWL30_001800"
     CDS             complement(3499..3729)
                     /locus_tag="IWL30_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706555.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENNQ
                     PVIAKKETVLSEGKIKTRYILEVETKFKNRSE"
     gene            4346..5008
                     /locus_tag="IWL30_001801"
     CDS             4346..5008
                     /locus_tag="IWL30_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706556.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            5087..8314
                     /gene="fss3"
                     /locus_tag="IWL30_001802"
     CDS             5087..8314
                     /gene="fss3"
                     /locus_tag="IWL30_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP9706557.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITDAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            8428..8742
                     /locus_tag="IWL30_001803"
     CDS             8428..8742
                     /locus_tag="IWL30_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9706558.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            8755..9129
                     /locus_tag="IWL30_001804"
     CDS             8755..9129
                     /locus_tag="IWL30_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9706559.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            9139..9483
                     /locus_tag="IWL30_001805"
     CDS             9139..9483
                     /locus_tag="IWL30_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP9706560.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            9554..10906
                     /locus_tag="IWL30_001806"
     CDS             9554..10906
                     /locus_tag="IWL30_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP9706561.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFWVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            10966..12108
                     /locus_tag="IWL30_001807"
     CDS             10966..12108
                     /locus_tag="IWL30_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAP9706562.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            12133..12387
                     /locus_tag="IWL30_001808"
     CDS             12133..12387
                     /locus_tag="IWL30_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706563.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            12643..13827
                     /locus_tag="IWL30_001809"
     CDS             12643..13827
                     /locus_tag="IWL30_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9706564.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            13842..13961
                     /locus_tag="IWL30_001810"
     CDS             13842..13961
                     /locus_tag="IWL30_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP9706565.1"
                     /translation="MEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKGK"
     gene            13966..14187
                     /locus_tag="IWL30_001811"
     CDS             13966..14187
                     /locus_tag="IWL30_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706566.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            14200..14700
                     /locus_tag="IWL30_001812"
     CDS             14200..14700
                     /locus_tag="IWL30_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP9706567.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            14797..16644
                     /locus_tag="IWL30_001813"
     CDS             14797..16644
                     /locus_tag="IWL30_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HAP9706568.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            16647..17543
                     /locus_tag="IWL30_001814"
     CDS             16647..17543
                     /locus_tag="IWL30_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9706569.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNFYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            17593..17982
                     /locus_tag="IWL30_001815"
     CDS             17593..17982
                     /locus_tag="IWL30_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9706570.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            17969..19471
                     /locus_tag="IWL30_001816"
                     /pseudo
     CDS             17969..19471
                     /locus_tag="IWL30_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
     gene            20216..22042
                     /gene="ltrA"
                     /locus_tag="IWL30_001817"
     CDS             20216..22042
                     /gene="ltrA"
                     /locus_tag="IWL30_001817"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP9706571.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
     gene            22176..>22977
                     /locus_tag="IWL30_001818"
     CDS             22176..>22977
                     /locus_tag="IWL30_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9706572.1"
                     /translation="MPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         7825 a         3715 c         4891 g         6546 t
ORIGIN      
        1 atcctattgc atgaaacagc aaacgaaact gcgacaggac gaaatgaagc gcagtacatg
       61 aaacgttctt ggtacaatgc ctatacagcg tatattgttg gcgatggtgg gatcgtttac
      121 caagttggac aacctggtta tgtacaatat ggcgctggtt catatgctaa tgcaaatagt
      181 cctgtgcaaa tcgaactgca acatacgcac gataaagtaa cgtttgaaaa gaactacaag
      241 gcatatgttg aattagcgag agattccgct aataaatacg gtattccact tacattagac
      301 acaccgtata atcaaccagg aatcaaatca catttatggg taactcaaaa catttggggc
      361 gatcacactg atccgtatgg atacttatca gaaatgggtg taagtaagga aaaactggct
      421 tacgatttag ctcatggatt tacagacgaa aacccaacga cttctgagaa caagcctgtc
      481 attgatccaa cccgagcagg tgcagcaaat ccaactttat cagatggaac gaaccattct
      541 cacattgatc agtttggaga aatcgaaaat gcgaacttac acgtcgctgg atggcacatc
      601 gctaactata aatacgagta tattttcatt atggactaca atactgggaa agaattagct
      661 cgagtaagag ctgatggaat ttatagaccg gatgtaaatc aagcttataa tacttctgga
      721 aacgttggct atcatgtatc ttttaacatg cgcaatttcc ctaataagaa agtatacgtc
      781 atgatgcggg caacgaatga tccagaagga aacactaaag gcggtgcgca agatttccat
      841 gacaaacgtt ggtatttaaa tattcctaaa cgataaaaat agctcctcgt tgaggagcag
      901 tacatagaat tgaaaattaa cgttaaatca aaaaaatatt tacaagatgt ttatttaagt
      961 agataataaa agaaaaaagc aggtgaaatt gtgacagcag aaattggtat tatgaataaa
     1021 agtggaatcg tgttagcttc agattcagca tctacgattg gagatagcaa agtatataat
     1081 actgcaaaaa aattatttac tttggattcc atgcattctg taggtatcat gatatatggt
     1141 aatgctgaat ttaatggtat tccttgggaa ataatcatta ctcaatataa gaaaagcatt
     1201 ggtagttctg tttttaatac tttggaagaa tacgcggata attttataga atttgtcaaa
     1261 acagcatctt ttattagaag tgaacagacc gagcaagaac aaatgattgg ggtttttcaa
     1321 aaaataattt caggtttatt tgagagtata gaagtagata ttaacttttt aataagccaa
     1381 ggtacacaaa tagacaaaga tgtcctagtg aagttattac aaacaaaaat gaataccaat
     1441 ttgtctcaac agtcccagac attcatttta gatattgaaa aggcgttatt tcttagtaac
     1501 tatggagaaa tattaaaaga tattcttaat agtatttcta caatggaagg tgtttcagaa
     1561 gctatatcag aggaaattca aagttacgtt tatgaaatta taattagaga cgatgtttat
     1621 tcttccccta caggaatagt tatagcagga tatggaagaa tggatatttt ccctaaacta
     1681 tattcttata atatgtttgg atttgttatg aatattctga aatactcaga atatgaaagt
     1741 gctcaaatag gaaatgacaa tggttcatta agatctacta tattaccatt tgctcagtct
     1801 gatgtggtaa atactgtagt tcaaggggta gatccacaaa ttactaatta tctgtcgagt
     1861 caagtcgata gttttgacga taacggaaaa aatacctata ttaatattat aaagaatatt
     1921 tcagagtttc aacaaaatca attcatattt ccgttactaa acatgattgc tttactccca
     1981 gttgaagaaa cagcaattat cgcagagact ttattaaatt taaccagttt taaacgaaaa
     2041 tatactactt ctgtagaaac agtaggtggc ccaattgatg ttttagctat tacacctaat
     2101 gatggcccta tttggattaa aagaaaacat tattttgata ttgataataa tattggatac
     2161 agattgcgaa aggagagagc gaatgattac aataattaaa cctcaggaaa aatcccctgt
     2221 tttgaaattt aatgagagca atctagccca taataaaatg agtataactg atacagtatt
     2281 tgatgtaatg gttgagcaac aaaaaaaaat taataataac aatagacaaa gaaaagaaat
     2341 taaccaatat taatgttttt tctgttgcgt tttttgcagt cataatttaa aacctcgctc
     2401 aatcttgggc gaggtctttt tttatttttc tgaaaagtta aagtaaataa tagcataaaa
     2461 taatatattt tacaaagaat aagtacaatc tagttttttg ctattaaatg tgtaataata
     2521 aatatgccat cacaacatga agaatgaaat ccattattat ctagtctagt ccattctttt
     2581 tgtttgcagt atttgtgatg gctttccgta cccttagctc agttggtcag agcagacggc
     2641 tcataaccgt ccggtcgtag gttcgagtcc tacagggtac attaacgtag ccatttgaat
     2701 cgttctgtgt tagaattttt tgaagagtat tatacaagct aaagcttttc ttcattgcca
     2761 ctcaaatgag tggctttttt atgtatcctt ttatggatta atgaaaggat gtttcacata
     2821 gttatatttc tgtatatttg aaaagtttta ctttgatttt taaatagaaa gacatttggg
     2881 ttaaattgtg agataataat aaagaagagt ttaaagcgca cctcaaacca cttccccata
     2941 agtgtgttac gctttaaact cttttatatt tgaagctatt aaaaggcata ccatattttt
     3001 gaaaaaaagt gagaaaaaag gcttacaatt ggagtggtag ttaattagtg acttattttt
     3061 gattttatag cactgatact ataaaatata gatatcatca tattacacaa tcttaatact
     3121 aacttaaaaa atatctcctt tcacaagtat ggtgataaaa ttcgttccgg gctacctttt
     3181 taggtagcct actttaatct ttataccttt ctggatcaac gaaagtatac tttatatagt
     3241 cataacgccg atgatcgcta cgtgcgtccg gcacgtcagt cacgatatca aacaaaaagt
     3301 atacatcttt cttcattcta gtttttgcag cagggatttt aaagtagttc ttattagaat
     3361 agtagagatt gattaataag ctatcttcga ttgctagaaa gaaaacttct gaatcccaca
     3421 ccttataaaa atctttgata aatctattcg aaggatcaaa tttaaaccat aattgtgtct
     3481 cattaaaaag catagccatt actccgatct atttttaaac ttagtttcta cctctaatat
     3541 atatcgagtt tttattttgc cttcagataa tactgtttct tttttcgcaa ttacaggttg
     3601 gttattttcg gaaaaagcta atatagctaa gattgaaaca tccatctgga atttatcttt
     3661 tttgctgctt tgctcataaa agtctgcata ttcatcactg atattttttc taataaagtc
     3721 ttccatcata aaaatcacct caaaacgatt atacgaactt acgttctcgt tgtaaagcga
     3781 tatttgaggg gcaaaaaggg ggcaaaaata cgtttacctt tgatatatct cagattgaac
     3841 atagtgttta ttatatgtcg gtattgttga tttatagctg ttttgagggt attagataca
     3901 taatagaata tagtggtttt gtttacgtgc attacacgga aaagctgctc gtatcaaaga
     3961 aatcagaaga taaacacgga aaacttcctt ggaaatctga aatttgtgaa aagtccttgt
     4021 atatcaaggg ctttttcttt tttctctatc acagttttaa gccatttttg gctgttttgg
     4081 accatttttc aatcatattc ccttaaaaat tgtcctagat tgtccgacat tgactatcag
     4141 tgtcgtcaaa atagggcaaa atttagtagt cagcttgacg acaaatataa tacgaaaaat
     4201 accaaaacat ttaccgaatt tagcaacttt tgaacccaag tttctcaaag ggtcagctat
     4261 tagcaaaaaa gacaaaacat gtacttttct actaaaaagt tgatgtgata ggcttctatc
     4321 taaataacta ttaggagttt ttatcgtgaa gcaaaatgat ggacgtatca tttggaaaaa
     4381 tcaaagtgag ttaaaactga ttttaacaat taatgaattc atcgaaaaac acggaattac
     4441 atctagccgt caataccaga aaaagctatc agaaaatcca aattctgcac caagtatgtg
     4501 gttcattaac aaaaaatatg gatcatggga gaatttattg attagtatag gacgagaaaa
     4561 tactgggtat ggaaaatggg ctagaatgtc agaacaagaa ttattggaaa tagtagaagc
     4621 ctttattaag tgcgagaaga taacctctca acgaatgtac gaacaaaaat cagtagggaa
     4681 gaatattcct tcacttagta ccattaaaaa gatgttagga gatatacgtc cactttttaa
     4741 agaaaaaaat gatggatctc gatttactga tttcgaactg ctgttagaac taaaaaatga
     4801 aataatccga ttaaaactac aggatgattt atcaatgaca aagtttcgaa aactagttca
     4861 atccccaaaa ctaccatcag ttgatacaat tatgaaaaga acgaacaaaa attgggagga
     4921 attgatggca gagattggat ttgattatcg aagaatcaaa atttataagc agagaaacaa
     4981 cctatccaag acaaagaaaa ctaaatagta tcactctaaa tcagacgttc aaaattaaaa
     5041 tttggacgtc ttttttatta cccaaaaacg aaaggagatt tattttatga aaaagccaag
     5101 atttaagaat tggcgtttgc tcgcaacact gagtctatta tgccaaacaa ttggtggatc
     5161 gcttggccca acgattgcct ttgctgatga aattactcat ccacaaacgg taactgtgga
     5221 attggatttg gctcaccagt atgctgtcga aggtacattt agcgatggtc gtcccatgtc
     5281 agaagttacc gttccgcact atgcagttta caatggtgtg aagcaagata ttttctgtat
     5341 tgagccaggg gtgccgattt acaatgagtt cactcctggt tatgagaaaa atccattgcc
     5401 tgatatgtcg gagaaagcga aattagtttc cgttttatgg aaggaagctg gtacggatgt
     5461 ggatacacat attgtggccc agaagatgat ttggcaagaa gttaatggct acacgcttca
     5521 ctcaattaaa cgttctgatg gtagtgcagt aaatatcgca gccattgagt caaaaatcaa
     5581 tcaagccatt gcggattatc aaaagaaacc aagcttccat aactccacag tgaaaaccgt
     5641 gttaggtcaa tcgactactg tgacagatac gaacggcttg aatttatcgg agtttgatga
     5701 agttgtcgag aatatcgcaa atattgatta tcgtgtgaac ggcaatcaac tagtgattac
     5761 tccaaatgcc aattcaaaag agagtggtga attaacccta aagaagtcag ctaatacagg
     5821 aacaccggta gcttataaaa tggctggtca acaaactttg atggcggggg caattgataa
     5881 gcccaatacc tacacaatca aaattgatgt ggaaactgag ggttctttga agattaaaaa
     5941 agtcgataaa gaaacaggaa atgttgtacc gggaaccgtt ttccatttag actttggaaa
     6001 aacattacct gcaaaagacg tgaccaccga taaagaaggc attgcgacat tggatgagat
     6061 tcctcatggc gcgaaagtaa ccattactga aaaatcagtg ccagctcctt atacgattga
     6121 cactacacca atcaccgcaa ccattaaatc tggtgagact atttctgtga cttcaaaaaa
     6181 tactcgcgaa aaaggtcaaa tcattttgaa taaaatgggg gtggaaacag gaactgatct
     6241 ttggaacgac aaatactcat tagccggaaa tacgtttgcc attcgcaaag acagtccaac
     6301 cggtgaaatt gtccaagaaa tgaccacgga tgaaaaaggt catgcggaaa caccaaaaga
     6361 gattgccaat gcgttggaat tgggaaccta ttatgtgacc gaaaccaaag ctagtaatgg
     6421 ctttgtgaat acctttaaac cagtaaaagt cgaattgaac tatgtcaatc aaaccgtggc
     6481 tcttgttacc agtaacgtaa aagggcaaaa ccaagaaatc actggtgaaa ccactttgac
     6541 aaaagaagac aaagataccg gtaatgaaac tcaaggtaaa gctgagttta aaggagctga
     6601 atatactctc tttactgcaa aagatggtca agcggtcaaa tggagtgagg cttttaaacc
     6661 agagttagtg aagggaacga aagcttctga tgaaacggtg actttgactt tagatgaaaa
     6721 gaatcaagtt gctgttaaac acttagcgat taatgagtat tactggcaag aaaccaaagc
     6781 acctgaagga tataccttgg atgaaacaaa gtaccctgtt tccatcaaaa aggtggatga
     6841 taacgaaaaa aatgccgtga ttacccgaga tgttacggca aaagaacaag tcattcgctt
     6901 tggctttgat ttctttaaat ttgctggatc agctgctggg actgccgaaa ctggcttcaa
     6961 tgatctgacc tttaaagtat cgccattgga aggaaccagt gagattacag atgccgaaga
     7021 tgaagcaatt acagcttata acgagcaact aggttttgat ggctatggca agtttgaaaa
     7081 tcttccttat ggggattatt tacttgaaga agtagaggct ccagaaggat ttcaaaaaat
     7141 tacgccatta gaaatccgtt ctacttttaa agaaaataaa gcggattaca ctaagagtga
     7201 atacattttt acgattaccg aaaaaggaca aaaacaacca attaaaacgg tgaccgttcc
     7261 ttacgagaaa ctgacgaata aagcattttc tgttagcttg aatcgtttga tgctctatga
     7321 tttacctgaa gaagaagata gcttaacttc tcttgggact tggaaagacg gaggcaaaga
     7381 attgacagac cttgattcta ccgagctagt tgataaattg agctataact tgcatgaaat
     7441 caaagaagac tggtatgtgg tagcgcaagc cattgatgtg gaagcgacaa aagctgccca
     7501 agaaaaagat gaaaaagcca aaccagtggt aattgccgaa acaaccgcaa cgttggcgaa
     7561 taaagagaaa acaggaactt ggaaaatttt gcataaatta accgcagaac aagttttgga
     7621 taaaaccatc gtcttattca actatgtgta tgaaaacaag aaaccctatg aagcaggtga
     7681 tgatccagta gcgaaagatg ctagcttgaa caatcaagcc caaactgtca attgtacggt
     7741 ggaacgccat gtttccatcc aaacaaaagc ccacctagaa gatggttcgc aaacctttac
     7801 tcatggggat gtggtggata tgttcgatga tgtgtcgatt acccatgatg tactggatgg
     7861 ctcaaaagaa gcttttgaaa caattctgta tgcattactt ccggataata cgaccaaaga
     7921 aatttggaaa tctggcaaaa ttgattatga agtgaatgac aaagaattca ctaaaaccgt
     7981 acttgcggaa aaagtggaca caagtaaata tccagaaggt acaagtttta ccttcaaaga
     8041 aattaactat ggcaaagacg gaaacatcaa tgggaaacac aatgaggatc taaaagaaaa
     8101 aactcaaact ttaacaccta aaaaagttcc gacaacacca agtgcaccag aacaaccgga
     8161 aataccaact gttccaagtg actctcaaga atctagtcct acagtaaaga cattcccaca
     8221 aacaggggag aaaaattcta atgttctgct gtttattggg tttaccttga tctttgcgac
     8281 tgctgggtat tatttctgga atcgccgaaa ctaaggtgat gtgatgaaac aactaaatag
     8341 gaaaaaccaa cgtgcgcgac cgcctcctaa aaaaagtggt cgcgctattt ttgtatccaa
     8401 aactaaaaat gaaaagagga aaagaagatg gaattgaaat ttgttgtgcc agatatggcc
     8461 gaaacctttg gaaagataag gtatgccggc gaaggcgaag ttttaactga aggatatggg
     8521 cggaatacta cagtaattgg tcgtagttat catttgtatt ccagtaagca acgagccgat
     8581 gatattgaag tggtggtagc tgcggaagcc ggcgaaaagg attttgatca agatcaacca
     8641 ctaaaagccg tgaatcccca tttggttgcc aaaggctatg agattgagaa tcgtgggttt
     8701 accgactacg tgttgtatgt cgatgattta gtgaaagcat aggaggaaaa gaagatgaga
     8761 ttaacagaag gtattgttgt agattcagga ctaacgtttg ggaaattacg attttccgca
     8821 ttacgccgag aagtacggaa gcaaaatgag gatggaacgg ttagtaacga ggtaaaagaa
     8881 cggacttata atttgaaatc ctctgcgcaa ggtcgaatga tccaagttag tatcccagca
     8941 aatgtaccct tacgcgagtt tgcctatgac gcagaggtag agttggtcaa tccaatcatg
     9001 gatacggtcg ccaattatgt tttccgagaa ggaactactg tcaattggtt cattaaggcc
     9061 gatgatttag tcttgaaacg acaaccagat cagggaaatt ctacaaacca aaatgaagga
     9121 aagaaatagg tgaagcgtat ggaaattaat gttgaacata ttttagattg tctcgatcaa
     9181 tatggaaaag gtgagctaac ggaggaacaa ctcactaaag cattaaccta tgatgaaaaa
     9241 atgtttctga ttatgcatca agggttactg gaagtaggta acgatgccga agaagatttt
     9301 gatgtactga attggttagg ggaagaagag tccttcttta tggttatcga agtaaatgaa
     9361 agtctatgtc gagaagccga aaccgtattg gaagaaattg gtgtggaaat gccggatgcc
     9421 attgaagttt ttttaaagca actagtggaa acgaaacaac tccctatagc agtaaatgac
     9481 tagcataact gcttgaaatt ggatcaatga aatcctatga agcttttttc gattggaggt
     9541 ggaaaccata aaaatgtaca aagggcatcg tatacgggca ggagatcagc acttggttta
     9601 tcactttgtt cttggctggc tactcgctct gttcatcggt tggatgagcg tcttttattt
     9661 tcaagagctt agacaatttg atatttctaa gctatcactt tctactatag aaatcgttcg
     9721 ttctataaaa gatttaatct atttgctagg aagtctcgtt ctttcaggaa gcacgatgtt
     9781 gctttatata cacttttttc aggatcattg gcgcagtttg tggcatcgac aaaagctagc
     9841 ccggatgatt ctagaaaatc attggtacga agtaaaacaa actcagagtg aaggattttt
     9901 caaagacctc aatagtagtc gaaccaaaga gacaatcagt tacttcccca aaatctatta
     9961 ccgtatgaag gatggtttac tttctattcg cgttcaaatt tcactgggaa aatatcaaga
    10021 tcagctttta aagctggaaa agaagttaga aagtggtcta tattgtgaac tagtagaaaa
    10081 ggaactcaaa gactcctatg tggagtacac tttactgtat gatataattg ccaatcgaat
    10141 tgggatagac gaagtagtgg cagaaagtgg ggctttgcga ttgatgaaaa atcaagtatg
    10201 ggcttatgat tctttacctc atatgttaat cgctggcggc acaggtggtg ggaagactta
    10261 ctttttactc actatcatcg aggctttatt aaaatctgat gccgagcttt ttgttcttga
    10321 tcccaaaaat gcggacttag ccgatttagg tacggtaatg cctcatgtct attcccaaaa
    10381 ggaggaaatt tctgcttgtg tggaagattt ctatgagcgc atgatgactc gtagcaaggc
    10441 aatgaaagaa atgcccaact acaaaacagg agagaattat gcgtttcttg gacttccacc
    10501 aaacttttta atctttgatg aatacgtggt ttatatggaa atgttaacga cgaaggaaag
    10561 tgcggtgatt ttgaataagc tcaaacaaat cgtgatgtta ggtcgtcaat ctggtttctt
    10621 tctgattcta gcttgtcaaa gaccggatgc gaaatattta ggagacggga ttcgcgatca
    10681 atttaatttc tgggtggctt tgggtcgtat gagtgaactg ggatattcca tgatgtttgg
    10741 cgaggttgat aaaaaattct ttatgaagcg tatcaaaggt cgaggctatg tagatacggg
    10801 agggagtgtg attagtgagt tttatacgcc acttgtacca aaaggatatg atttcttaag
    10861 ggaaattagc gacattggtg gattaaacgt taatacggaa ggataaaata atagtatgta
    10921 gcctaggagt gtcaagaatc ctgggctttt tttacgaggt gatttatgac atttttagat
    10981 ttattcgctg ggattggtgg cttttgctta ggtatggaac aagcgggtca ccagtgtatc
    11041 ggcttttgtg aaattgatga ttttgctcgc cagagttata aggcaatcca tgatacaagt
    11101 aaggaggtgg aaatgcatga catcacaagt gtatcagacg agtttattca atctctcgga
    11161 ccagtggata tcctttgcgg tggatttccg tgccaagctt tttcaattgc gggaaagcgg
    11221 caaggatttt ccgatactcg aggtacttta ttctttgaaa tcgctcggtt cgccgctatt
    11281 ctccaaccta agtttttatt ccttgagaac gtccggggat tactcaatca cgaaggaggg
    11341 gctacgttcg agacgatcct ccgaacgctg gatggattgg ggtatgatgt ggaatggcaa
    11401 gtgcttaact caaaggccta cgttccccaa aaccgtgagc ggatcttcct tatcggacat
    11461 tctcgagacg catgtaccga acaagtattt cctatcattg gatcgtctcc aacatctgat
    11521 caaaacatca gaaacttatt aaatatcaac ccttccaatc gaggaatggg gggacaagtc
    11581 tatgggtcag atgacgtagc gcctaccttg accggtgatg aaggaatcaa aatcgcattg
    11641 ccggtagatg atggaatttc tgtagcgggt atgttactgg gaaattttga acaaggcaat
    11701 cgggtttatg agataactgg aacagcgcct actttgtcaa cgaaacaagg cggaacgaag
    11761 atcatgcttc ggaaaaacga aacagaatat caagaggtga agccagggaa cagtgtgaac
    11821 ctggcttttc ctaattccac aagtcgtcga ggccgtctgg gaaaacaaag tgtgcatacc
    11881 ttattaacag gagaccaaca agcagtcgtc acggatcagt atcaaattcg aaagttaaca
    11941 cctagggagt gctggcggct tcagggcttt ccggactggg cttttgatcg agcttctcaa
    12001 gtcaattcag atagtcagtt gtataagcag gctggaaatt ctgtaacagt tccggtcatt
    12061 tttgatattg ctagaagatt aaaggaggta aaaacagatg atttatgatg agttgattgg
    12121 ggaatctact gggtgattgg caagattcag tcagatccgg aattggaaga agaactgtac
    12181 cggctcaatt ttgatattcg caaaaacggg gtgaaagtcc ctgatgatcc gtatgtaatt
    12241 gatgaggaaa cggatgctcg aatggagctg aatcaagtca tcagtgaatt ggaacgaatt
    12301 gcggactttg ctaaagaacc tgatatccgc caatacttgt ttgagataaa agcagagttg
    12361 gaaattgagg gtattaccgc ggagtaacgg ccaaataaac cactgatagg cactgaccgc
    12421 gggggatttg cgaagcagaa atcctccgtg gtgctgtgcc tgtttatgga cggcgttagc
    12481 cggccatgat gtctcacccc ccgtatctaa caggggggta caaaaacaca aaaaataagc
    12541 aagcaacgat aaggaatggc tgtcctatca aggataataa gcaatttgtg tagatgttaa
    12601 ctgatttgaa aaagttaaca ccttaaattt ggaggtgaga acttggcaca aaggaattta
    12661 gattatcggc tattaaaaga ccggcgtaat gaatatggcg tttcacaaaa taaattagct
    12721 acgacttgtg gacttagtcg cccgtactta aatcagatag aaaatggtgg cgtaaccgca
    12781 tcgacaaaaa ccatgaggaa aatttttgac caactggaaa gtttcaatcc cgagttacct
    12841 ttaacgttac tgtttgacta tgtaaggatt cgctttccca caacggatgc gaggaaaatc
    12901 attcaagaga ttctccattt gaaatttgat tatatgctcc acgaagatta cgccttttac
    12961 tcctatcaag aacaatatgt tatgggagat attgtggtga tgttgtcaca tgaagaagat
    13021 aaaggcgtcc ttttagaatt aaagggtcgt ggttgtcggc agtttgaaac ttttttactc
    13081 gcccaaaagc gtagctggta tgactttttc gaagattgtc taaaagccgg tggcgtgatg
    13141 aaacgcttag acctggcaat caatgatcga gttggcctgt tagatattcc tgatttaacg
    13201 aaaaaatgtc agaaggaaga atgtatctcc ttgtttcgta ccttcaaaag ctatcgttcc
    13261 ggggaactgt tgaaacctga tgataaagat ggtatgggca atactttgta tattggcagt
    13321 cttaagagtg aggtctattt ctgtttgtac gaaaaagact atgagcaata tatcaagtta
    13381 gggattcctt tagaccagac cgaaacgaaa aatcgttttg aaatccgact gaaaaatgat
    13441 cgagcttatc atgcaattca agatttgtta aaaggtcgca gtattgaaag taccacattt
    13501 tccattatta accggtactt gcgatttgcg gataaggtgg aaggcaaaag gcgaactaac
    13561 tggcccttga atgaacaatg ggggcgcttc attggacgga atcagaagga aattcaatta
    13621 acttctgaac cgaagcctta taccattgaa cgaacgttga actggcttgg acgacaagtc
    13681 gcccctacgt ggaagatggc caaggagttg gatcggttga atcagacaac gtacattcag
    13741 gatatggtaa ataatactcg actatcagac cggcacaaaa agattttgga gcaacaaagt
    13801 atggcaattg aaaatttgat tatatgaaaa gaggaatcgt gatggaaatt gtgaaagata
    13861 ttttattggt gtttggtggc agtatgcttg gtgttactgt gatgtgcttg atgcatgcaa
    13921 gtgcagcggc ggaccgagca atggaaaaga aaggaaagtg atgaaatgaa tttcggtcaa
    13981 aatttgtatc aatggttttt aaccaatgct caatctttag tcttactagc gattgtggtc
    14041 attggtttgt ttttaggatt taaacgcgag ttttccaaac tgattggctt cttggtgatt
    14101 gctttgattg cggtaggtct ggtctttaat gcttctggtg tgaaggatgt cttactcaat
    14161 ttgtttaacc gaattgtcgg tgcctgaggg ggaatcacaa tggaacagat acgtgtttat
    14221 attgcgaacc ttggaaaata caatgaaggt gagttggtgg gggcatggtt taccccaccc
    14281 gtcgattttg atgaagtgaa agaacggatt ggattaaatg acgaatatga agagtacgca
    14341 attcatgatt atgaactgcc ttttgagatt gatgagtata caccaattga ggaaattaat
    14401 cggttgtgtg gtttagcgga agagcttaaa ggaacgccaa ttggtgaggt cgcttcagag
    14461 attcaacatg ccttcttcaa ttctttcgag gaaatggtag agcatgtgga tgatatcatc
    14521 tattatcccg attgtaatga catgagtgat gtagcgtatt acttgattgt agaagctggt
    14581 gatttaggcg aagtaccaga acacttgaag acgtatattg attttgatgc gtgtggtcga
    14641 gacttggaga ttggcgggaa ttatttggtt acgagtcgag gggtttttga actaatgtaa
    14701 atcatttgtt agaattgacc agaaatttct atcgtacttt tacttttcta tttgatatac
    14761 tgaaatagaa aatatttgtt gataggggtg ttactaatgg ttggcaataa gtggtacaaa
    14821 tgtgatcttc atattcattc aactgcgagt cagtgctttc aggataaaaa ggttactgct
    14881 aaggaatggg tagaagaatg tttaaacaaa ggactcgatt gtgttgcatt aactgatcac
    14941 aatacagggg aaaatattga tgaatataag aaagtggcta tcgaaaatgg cttagatttt
    15001 tttcctggag tggaattaac gtgtggagaa aatggaactc atttactagt cctatttgat
    15061 aaaagtgcat gtacaaatga tatctctgat ttcttaacca agatgggtat aaaaagagag
    15121 gattgggcca gcgctgcagc ttgctcaatg aaatcagttt tagaagttat tgattttgct
    15181 gagcacgagg gcttgctcgt tattcctgca catatagatg agtttaatgg aatttcttca
    15241 gcaaaatttg gaaatctagg aaagatattt gaatcagaaa atatatatgc agtacagacg
    15301 gttcaaaaag aattttttga aaatcgctca caaatgattc cctcatctaa gcgtgacact
    15361 atctatgata gtgtgaatgc cagatatgat ggtagagttg gaaaagatac tttggaatct
    15421 tggtataaat cagttgttga ggcagaaaaa aataatatga catttcttag cttttctgat
    15481 aacccacact cgaaaggaaa ttcaaaacat ggactttggg gaataggcac aagatattcc
    15541 tatataaaaa tgaaggacga acctgacttg ggatcacttc gcgatgcttt aatgctaggc
    15601 gaaactcgag ttcattctga tttttctaat ttctccatta atgaaaatga agttttattg
    15661 gaaaaattaa gcttttctgg gaccactctt agcacaaaag aagttgtagt cgaattcagc
    15721 gataatttaa cttcaattat tggaggaaga ggaacaggca aatcttgtat tacaagattc
    15781 ttggtctatg tccttggaaa ggaagcagaa cttgatcaat tttctgaaat acaaagtgat
    15841 tatcagaact ttgcacaaat tgaacataat ggctctggaa tatttttgaa ggatacaatt
    15901 gtgaaactga atattttcta taaggggaca aaatatcaaa taataagaac acaggataga
    15961 cattctattt atgaatttac acaagagaat ggtctggttg aaaaagaaac agaacggttg
    16021 agaatgattt ccgataaggt gagtatatac tctcaaaaac aaatttatga aatttcaaaa
    16081 aatcaaacta gtatactaga attactagat ggatataact ctgatttgat ttcagagtat
    16141 aaaaatgaga ttgaaacatg tgtaaacgaa ataagaaaat taaattggga tattgtttct
    16201 gtgaaaaaag aaatacaaga taaggcgaag gtagagcttg aaattgaaga tttaaaaaaa
    16261 caagtagaaa agctatctca taagagttat aaagatgttt atgatgagta ttcaaaagag
    16321 acagatattt atagggaact gaaacgtgat gtggaggcac ttaaggaaat tcccaaaaat
    16381 ctaactgata gtgttgacaa aattgacttt ggcaatggta taaaagttac agacgaaatt
    16441 gatgaacacc gcggagaatt gattaagaat ttagtgacca atcgggctaa gattcaaaca
    16501 ataattaatg aaatgtcaga tgaaattgat agttatagag cagagctgaa taaatcagat
    16561 tggaaagtta actataatga ggtttcaaag aggtatcaaa gaggtatcaa aaacttagtg
    16621 aagagttatc tcaagatgaa ttaaaaatgt tacaaaacct tgattcttta tatcaatctt
    16681 tatcaaaaac tgaagagcga ttggaagaaa ttattcaaca agaaataaag cttaaagatt
    16741 accaagttga ggttgactca gcaatgtcaa gaatgaataa atcatatcaa aatttctatt
    16801 ctgagagaag tagttcctcg cgcgaaattt tttctgatat tacaggaata gaggctacta
    16861 ttcaaccaaa tagggatttc aataattatg ttaaagagct cagagcttgt attaataagg
    16921 aggacacttt tgacagtgag tttggatctc ttattgataa gctgacaaaa aattccatta
    16981 ctaatcaaga tatatataat gacatacttg atataagagt tagtggggaa agtagtattt
    17041 tttcagataa aaaattaatc aattctataa gaaaattgaa tgatgcacaa gttcttgaat
    17101 tacaaaccct taagccaaat gatttagtag ttatccgact tgaactaaat ggaaaaaaag
    17161 ttagtttatc gaatgctagt gctggtcaaa agacatctgc aattctttct atgattttgg
    17221 cgtatggaaa ttcaccactc atattggatc agcctgaaga tgatttggat agtcaattaa
    17281 ttaataactt aatcgtaaga agtatcatta caaaaaagga aaacagacaa attttaattg
    17341 taactcataa tgcaaatatc ccagttaatg gtgattcaga atggttgatt agcatgggag
    17401 attcaaaaga aatatcggct gatcagtgcg ggtcggtaga tgaacccaat atcaagccaa
    17461 gaatatgtac tgtgatggaa ggtggcgagg atgcttttac aaatagagcg aaacgctatg
    17521 gttttaaaca aattgtagaa taacactaga gggtgagaca tattattatg tccgcccttt
    17581 ttaggaggaa aaatgaaaaa aataaaaagc tataccagta tctgggcagt tgaaaaagtc
    17641 atttacgcaa tcaatgattt tcagttgcct tttcctgtga cttttaacca aatggcttgg
    17701 ttcgttttat ctttactgtt tgtcatcgta tttgcccatg tgcctccact atctatgata
    17761 gaggaggtct ttttaaaata cttaggcata ccggtggctg tcacttggtt tatgtcgcaa
    17821 aagacgtttg atggcaagaa accactggga tttttacgat cctttatcag ctatcacttg
    17881 cgatttaagg tgacttttgc aggaaagaaa gtcaaagaac agaagaaacg atgggatgaa
    17941 cccagtactc ttgtaaggag tgtgaactat gtatccgatt aagtatattg aaaataatct
    18001 cgtctttaac caagaaggcg agtgttttgc ctactatgaa ttagtgcctt acaattactc
    18061 ctttttgtca cccgaacaga aatttcaagt gcatgataac tttcgccaac taattgcgca
    18121 aaatcgtgaa ggcaagatcc atgcgttaca aattgccaca gaaagtagta ttcgcgccac
    18181 acaagaacga tcaaaaaaag aaattaccgg tagactcaaa gaagtagcga aacaacgaat
    18241 tgatatacag acagatgcat tagtatccat gattggcgat agtcagattg attatcgctt
    18301 ttttattggc tttaaactaa ttgcgacaga tgaagaagtc aatctaaaga gtctcaagaa
    18361 atctttcttt tctgggcttc aagaatttgt ctatggagtg aatcatcatt tgatgggcga
    18421 ttttgtttcc ttatccaatg aagaaattcg ctgctattct aagcttgaaa aattaatgga
    18481 aagcaaattg gctcgaagat ttaaagtcag gcgagtcaca ccgagtgacc tgacgtattt
    18541 gattgaacat atttatggcg aaaaagggat accgtttgaa gaatacgaat ttcagctacc
    18601 aaagaaaaag ctaaaatcag aaacactggt gaaacgttat gacttattac gtccaaatcg
    18661 ttgtttaatt gaagaaaaac caaggtgctt acggatggaa catgagaatc atgagtcgta
    18721 cgtagcgtat ttaacaatta atacaattgt gggggagatg gaatttcctt cctcagaact
    18781 tttctattac cagcaacaac aatttacatt cccaatcgac acaagtatga atgtagagat
    18841 tgtgacgaat aaaaaggctt tagctaccgt tcgtaataag aaaaaagaac tgaaagattt
    18901 agacaatcat gcttaccaat ctgacaatga aacgaattcc aatgtcttag atgctttgga
    18961 ttccgtggat gaattggaaa ccaccttgga tcagtcaaaa gaatctatgt acaaactgag
    19021 ttatgtggtt cgagtgagtg ccgaatcagt ggatgaacta aagcgacgct gtgatgaagt
    19081 gctcgatttt tatgatgata ccaatgtaaa actagtccgg ccctttggcg atatgatggg
    19141 gctacatgaa gaattcttac ccttaagtaa acgatatatg aacgactata ttcagtatgt
    19201 tacgtctgat tttttggcgg ggcttggttt tggggctact cagatgctag gagaattaga
    19261 agggatttac tttggctaca atgtggatac aggtcgtaat gtttacctga aaccggcatt
    19321 ggcttcacaa ggagtcaagg gttcagtaac caatgcgctg gccgccgctt tcctgggttc
    19381 tcttggtgga ggtaaatcct ttagtaacaa tctcttagtc tactatgctg ttttgttcgg
    19441 tggtcaagct gtgattgtcg atccaaaagg gggtgcgaaa cgttcctact tgaaaagagt
    19501 aggcacagca cttcattaaa tcaatattta atgtgtgttg gagaactaaa cacacgaata
    19561 gtcgaatgac ggggtaacgc cctgaaaggc tactttaata ctctgactgg cagaaacttg
    19621 agtaaacaag gctctgtcag aagctcggtg aagtcggcag acagacaccg taacagataa
    19681 tgctgacgaa agattcccag aggtgggcgt tgtgtcctat atgccggaag tctataaaat
    19741 atctatggtt agaatgtaga tgagaggtct actgacgaat ccttgaatgt acaggtctat
    19801 atcggggctg gatagaaatg tccagtgaca caaacagtgt cgctactgtg ggtgagtaag
    19861 actccgcatt tatgaaaatc catataacgt taaaggcgtt atcaaggtag caggcttata
    19921 gaggaaacct aaggatatat gtacagatag tgtgtttgga acgttgaaag ctgacaacat
    19981 ggagatttta ctgtctgtga agtgttcaca tcagaaagtt attgtgagat tagcaatgat
    20041 ataaggtgtg ttaatgtcag tgagagtggt ggcacagtac ctatgaaacc gtgataacaa
    20101 gcggtggagg gatagccact agtcatatga agaatagaat tactaagaaa aatggtatgg
    20161 gttcgagtaa gactaagaga tgtacttgct cctgcaaaca aggagggtac agaccatgct
    20221 aaagaaaaac aagctccgct ataacgagta ttatgatatg caacatattt atgacgagtt
    20281 atacgcacag agcaagaacg gtaataactt ttataagtta cttgaaatta ttggttcgga
    20341 acaaaatatt tgtttagctt accgtaatct gaaatcaaac agtggcagta aaacggctgg
    20401 aacggatgga atgacgatag acgatataaa acaactcagc aatgctgaga ttgttgctac
    20461 cgttcgggaa agccttagca actaccgccc caaatcagtc agacgtgttt tcattccaaa
    20521 agctgggagc gataaaatgc gtccattggg gattccgtgt atctgggaca ggctggtaca
    20581 acaatgtatc ctgcaagtcc tagagccgat atgcgaacca aagttccata atcattccta
    20641 tggattccgt gcgaatcgaa gtgcccacca tgcagtcagc agagtaacga cactgataaa
    20701 tttatcaaaa tatcattact gtgttgacgt agacattaaa ggatttttcg ataatgtcaa
    20761 tcatggaaaa ctattaaaac aaatctggac acttggtatt cgggataaaa gattgatttg
    20821 cattatcagc aaaatgctga aagccgaaat tgacggcgag ggtgtcccag aaaaaggaac
    20881 gccacaaggc ggtctgttaa gtccactgct ctccctcata gtccttaatg aattagactg
    20941 gtgggtaagc agtcagtggg aaacattcca gccgaagaac cgcagtaaaa atgggtggct
    21001 tcaatacgca aagaaatata ccaaactgaa aagcggtttt attgtacgtt acgctgatga
    21061 ttttaaaatc atgtgttcaa cctatggaga agcacaaaga ttttaccaca gtaccgtaga
    21121 ttttcttaat aaaaggctga aattagaaat cagtccagag aaatcaaagg tagtaaatct
    21181 gaagaaaaat tcatcagatt ttctggggtt caaaatcaaa gtcattccaa aaggaagaac
    21241 gaaacacggt tatgttgcca aaacagatat gaaccaaaaa gcattaaaga aagcgaaaac
    21301 aaatctaaag ttaaaggtaa aagatatagc cagacatacg actggcttca atatcagcag
    21361 atataatctg acagttatcg gtatgcagaa ttactactgc atagcaacta acgtatataa
    21421 caacctgaca gaagtaagtt acgctctctt accgaccata cgaatccgtt taaggaacat
    21481 tgcaaaatca gttccatttg aaagtacaag ttcagaattt cagagcagga caaaaggaat
    21541 cagaccaaaa acaaaaatag tgatgattgc ggataatccg ctattgccga ttcaaggagt
    21601 acaacacaaa aatcctatga atttctcgca agatatttgt aattttacaa agcaagggag
    21661 aaacaaggtg catgaggacg ttgtagttgt aacaaaagaa gaaatccgag cgttgttgga
    21721 aaatgaaaac cctgccgata gcgtagagtt taacgacaat cgtatttcgg catatatcgc
    21781 acaacaaggc aactgctatg taatgaatcg tcgaggaact ccgtccactc tgatatgtat
    21841 tcacaaatca gacaaaggag ataaccttga cagatattct aatctggcat ttgtggaaat
    21901 tcccattgca aaggcaatcc taacagaatc agcagacgaa gcaaaatcac tgttaaaagg
    21961 ttatgttctt aatagtcagc agaagaaaaa actgaacaga atacggacga attacggata
    22021 ccagacaata actgggaaat aacttataaa tctatttgga tatgatggaa cgccgtatga
    22081 agcgaaagtt tcatgtacgg tgtgaagcgg gggaaaactt ggagataaca tcaaaaagtt
    22141 acctatcgct atagcgagga gggtggaaag aaaccttgcc agaaattgcg gatgaaatca
    22201 gtatcctcaa cttgacgagt aagtcagaaa atcaaggtct gcttgatcct tatgtgatta
    22261 tgaagaaaaa gaaagactcg gaaagtttag caattgatat tctgactttt ttaacaggaa
    22321 tttcaagtcg agacggggag aagtttcctg tgttacgtaa agccattcga acggtgactc
    22381 aaagtgatca acgggggtta ttacaagtta ttaacgaatt gcgaaaagac gggtcaccaa
    22441 tcgcggaaaa tatcgccgat catatcgagt cctttaccga ttatgatttt gctcatttgc
    22501 ttttctcaga cggctcgatt acccaatcga ttagtttaga taaacagcta aatatcattc
    22561 aagtggcgga tttggtttta ccggatgcag aaacgacttt tgaagaatat accaccatgg
    22621 aattgcttag tgtttcgatg ctaatcgtca tttccacttt tgccttggac tttatccata
    22681 gtaaccgaga aatcttcaaa attgtcgatt tggatgaggc ttggagtttt cttcaagtgg
    22741 ctcaaggaaa aacgttatcg aataagcttg ttcgtgctgg tcgggccatg aatgctgggg
    22801 tctatttcgt cactcaaaat agcgacgatt tattagatga acgtttaaaa aataatatcg
    22861 ggttgaagtt tgcttttcgc agtaccgata ttcatgaaat caagaaaacc ttggaattct
    22921 ttggtttgga tcaggaggat caaagcaatc aaaaacgcct gcgagatttg gaaaacg
//
LOCUS       DACDUU010000048        22351 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.048, whole genome shotgun sequence.
ACCESSION   DACDUU010000048 DACDUU010000000
VERSION     DACDUU010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.048"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(38..1189)
                     /locus_tag="IWL30_001819"
     CDS             complement(38..1189)
                     /locus_tag="IWL30_001819"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP9706573.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1202..1639)
                     /locus_tag="IWL30_001820"
     CDS             complement(1202..1639)
                     /locus_tag="IWL30_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9706574.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1654..3420)
                     /locus_tag="IWL30_001821"
     CDS             complement(1654..3420)
                     /locus_tag="IWL30_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAP9706575.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3684..4361
                     /locus_tag="IWL30_001822"
     CDS             3684..4361
                     /locus_tag="IWL30_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9706576.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4364..5491
                     /locus_tag="IWL30_001823"
     CDS             4364..5491
                     /locus_tag="IWL30_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9706577.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5691..6131)
                     /locus_tag="IWL30_001824"
     CDS             complement(5691..6131)
                     /locus_tag="IWL30_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9706578.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6142..8175)
                     /locus_tag="IWL30_001825"
     CDS             complement(6142..8175)
                     /locus_tag="IWL30_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9706579.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8177..9880)
                     /locus_tag="IWL30_001826"
     CDS             complement(8177..9880)
                     /locus_tag="IWL30_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9706580.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9974..10924)
                     /gene="manA"
                     /locus_tag="IWL30_001827"
     CDS             complement(9974..10924)
                     /gene="manA"
                     /locus_tag="IWL30_001827"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAP9706581.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10963..11988)
                     /locus_tag="IWL30_001828"
     CDS             complement(10963..11988)
                     /locus_tag="IWL30_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP9706582.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12197..13066)
                     /locus_tag="IWL30_001829"
     CDS             complement(12197..13066)
                     /locus_tag="IWL30_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAP9706583.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13525..15417)
                     /locus_tag="IWL30_001830"
     CDS             complement(13525..15417)
                     /locus_tag="IWL30_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9706584.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15414..17141)
                     /locus_tag="IWL30_001831"
     CDS             complement(15414..17141)
                     /locus_tag="IWL30_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9706585.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17157..17807)
                     /locus_tag="IWL30_001832"
     CDS             complement(17157..17807)
                     /locus_tag="IWL30_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9706586.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18011..18307
                     /locus_tag="IWL30_001833"
     CDS             18011..18307
                     /locus_tag="IWL30_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP9706587.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18479..19099
                     /locus_tag="IWL30_001834"
     CDS             18479..19099
                     /locus_tag="IWL30_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAP9706588.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19191..20357)
                     /locus_tag="IWL30_001835"
     CDS             complement(19191..20357)
                     /locus_tag="IWL30_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9706589.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20369..20722)
                     /locus_tag="IWL30_001836"
     CDS             complement(20369..20722)
                     /locus_tag="IWL30_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP9706590.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20850..21872
                     /locus_tag="IWL30_001837"
     CDS             20850..21872
                     /locus_tag="IWL30_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAP9706591.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
BASE COUNT         6482 a         4586 c         3957 g         7326 t
ORIGIN      
        1 aaaaatttcg tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac
       61 ggttcacgtt ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt
      121 gaatcaattg ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat
      181 cttcaggatc atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca
      241 agttatgttc ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac
      301 gtaatggtgt ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat
      361 gaatgatttt ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca
      421 gcaaacttcc tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca
      481 ttgcttcatc aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac
      541 cagtattgac actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa
      601 ccccttttaa ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga
      661 aaggttccac ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga
      721 ctgctgcatt tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat
      781 acacttcttt ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta
      841 aagatgttgt gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa
      901 taaaaggtaa gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt
      961 ggattacttt ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt
     1021 tttcttttga ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa
     1081 tgatcgtgtg attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc
     1141 caataaatcc tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc
     1201 cttaagcaat tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga
     1261 caacattgtc catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat
     1321 ctccgactcc tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa
     1381 aatctacacc atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg
     1441 cttcatctgt tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt
     1501 ctctatctac catggcatcg atatattcct catcaacgca ccctgcttct acaagcatct
     1561 gaccgcaata gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt
     1621 cgaatggcaa actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc
     1681 aatcgattcg tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg
     1741 tgcagccatc tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc
     1801 atcttttaaa tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa
     1861 tatatctttg agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag
     1921 tagaatctct ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc
     1981 aatttcagaa actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg
     2041 aggtgaattc aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc
     2101 tctttcttgt agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct
     2161 gattgcttga ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat
     2221 cgaagcgccc atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac
     2281 gccagagaat gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc
     2341 ctttgtctct tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa
     2401 aattactgca gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg
     2461 aaggtaagaa ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg
     2521 cgctcttagt ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat
     2581 aactgataag aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc
     2641 gcctaaaaag tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa
     2701 caaagtaaaa gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag
     2761 aatagatttt ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt
     2821 gattgcatta ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat
     2881 caaatgggca gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac
     2941 aactggacca atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga
     3001 aaaattattg actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa
     3061 tttcttgatg gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat
     3121 atctgttccg acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc
     3181 agcaattact tttccgccag tataaccgat caataaagga atcagataag ttagcattgg
     3241 gcttaccatg gtagccaaag cctcatttgg taaataacca tctgggataa atagagccgt
     3301 taatacaccc caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact
     3361 gcctaatttt tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat
     3421 tttgtctctc cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat
     3481 agtcgtactt tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag
     3541 atttcggtat cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata
     3601 tgcaaacaat aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta
     3661 aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg
     3721 gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt
     3781 tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc
     3841 caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc
     3901 gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg
     3961 attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc
     4021 aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga
     4081 aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg
     4141 aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat
     4201 ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga
     4261 tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg
     4321 aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt
     4381 tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt
     4441 gctctttgca ttcccacttt tttttgatca taaacttgct tatacagaat tcgatgaact
     4501 gaatcataac tatgaagaga tgcaacgttt gattgaaacg ttgaaagaag atgagaatga
     4561 gaaagaattt gtggaaagtt tagagaaaag caacaaactg atcgaagcaa tcttacatgc
     4621 aaaaaacaca gggaatgtcc agcaaatggt cgaagcaact tatcattatg aaaaagatat
     4681 actggatcga ctgatttctg gtcaaagaca aggaattcca atcattgaac aacaaaaacg
     4741 tgtcgagttg ctccgttata tgaaagaaca tcagatccaa agatattcta tttttgactt
     4801 gccagcgcat ctccctttag caaattacta cgaaaatatt ttttcaggaa tgatttctag
     4861 ttttttgatt ttatgtataa ccgccttgtt tctttcttcc atcatctctt atgaaaaaag
     4921 aaagcaggta atctctctag tcaatctatt accggatagt atggtaaaga aacatagcat
     4981 ccgttttacc atttattatg gtgcagctat gctaagtctc gtgatgccat ttttgatcgt
     5041 ctctatacta gtaataatca aaaatggttt gggagacttc cgttatcctg taggaacgat
     5101 cattggtcag gagatacgaa tccttccgat gtatgagtat cttttccaga gctttctgtt
     5161 cctgttgttg tgggtgctct ttttaagtac aatcagtttt cttctatctg ctttatttga
     5221 acattctcta gtcaatcttt taggtacact tctttgtctt ttcttagcag agtatcgctt
     5281 gttttcttcg attggatgga tagaatcaat cagtcattac ttgccaactt cttatgttga
     5341 ttttcaaaat gtgatcatag gaggagatat tttctcgcca ctagcttcag aacaggttac
     5401 tttcatgaat ggtatcttga cacttggtat atggagtatc gttttattat tcataggtat
     5461 gggcactatc tatataaaaa agagctatta aaacacaaaa ataatagggg cggtgacaaa
     5521 aaccttgtca ccgtccccta tttcaaataa acggttttct agaataaata gttttcaaaa
     5581 gcttgcatcc gcagtaataa gaacctccca acaaaaatag ctcctcttat ttttatcttg
     5641 gttgaagtta ttacttgatg caaggcagct ttttttactg tgaacattgg ttatatatgt
     5701 ccatgttcaa aaatcatttg gacttcttca atggttttcg catcactgag cgccattact
     5761 tggtcgatgt ctgaacagaa aaaggctaaa tcttggagca ccgctaactg atgttctttt
     5821 tttaaagcca ctacaaataa gagtgtcgcg atttttggtt cctcttttgt tccaaagtcc
     5881 acaccatcag gcacttggac taaatagatt ccttcttcta ctacttcttc atctttgtca
     5941 gtcccatgtg gcaaggctac gaaattcccg atatagacac tcgctttttc ttgtctttcg
     6001 atcattgatt ggatatatgc cggtttgatt cccccttgtt gggtcatcca ctggccgatc
     6061 tcggtcaacg cttctttcca attttgatag gattgatcga gcaatatttt ttctgctggt
     6121 agctgtatca tcttttcacc ttcatttctt tatttcttca atcaggattt tcgctaacaa
     6181 ctgataaacg atttctttat ttcctgagcg gaagatctcg gtgttcaagt cattcatgat
     6241 gattgctcca ctgatctttc ctaaaagaat cccgtctttt tcttggattg gcgaaggcgc
     6301 cagcataact aacatacggg acaaatcgac tggctgtcta tccatccctt caatggtcaa
     6361 cggatgtttc aaattgaaaa tacaaaagat cggtttagaa atcaatgagc tggatgtatg
     6421 gaataacccc atatttgtat gaggaatacc gataggtgct tgttcatatc gcttaagcag
     6481 ccggcgatga accacctctt catccgttac tagtccttca ggaaaataag gaaataagct
     6541 gcgaatcgtt tcggaaatcg tctcaggatt atccagttct tggataaaaa aacgaccgag
     6601 taattgattg atttgatcca tgaaatgcat tgtttcctca taagtatctt ctatcatcca
     6661 ctcgttattg ctgttatacg taattttttg cctttttttc ttctcaatat gcgaaaaagc
     6721 ttcttttagc tgcttgactt catcttctag cagtagcggg gagactaaaa tatattttcc
     6781 accgtatcct ggtaaaatag atgtagaaat aacaacatca tattcttctt gtaaatcgat
     6841 tttttttaaa tcagcaatcc gatagaaatg aatttgttgg atgaagggaa aatacctttt
     6901 caatcgtagt tccaacatgc tagtagaagc taatccactt ggcgaaaatc ctgcaatact
     6961 gatattgctt tctttcggac ttcgttccaa tgaattcgca aaatgaagga ccatataagc
     7021 gatctcttct tcggataatt ttttttcttg aaaaatccgc tctactgctt tacggattgc
     7081 attcgcaact tcctcgtatt gaaccatgat tttttctaaa atcggattgg tcagtgtctc
     7141 ttcttgtaaa acggctcttg aaaacacacc agataaatga gtcaacagca ttttgtacaa
     7201 agtttcatct tcataaaaat ggacattcgt ctcttcactt acttcttgaa tcagttgttt
     7261 tactaaataa gcaagctgtg tatcgaacgt ctcttcaaag aaattctgat caaatgagtt
     7321 cgtgaagtca tttaataatc ctgcaaaaaa gacgatctca tttactggat aaagttgttt
     7381 tgtctcagaa gcgatccgtg aaaacaatcg tttagctgtc tgtagtaatt ctttgtttgc
     7441 cagactatta tatgtctcat ttgtcagtaa atgattttta gtcacacgct ccatcgtcaa
     7501 tgtcagcatc aaaaccaaat gttcaagctt tctatcactt agattggtct ttttttggat
     7561 caattcttcc ccgattactt tgcgtgcaag agatagatgt tgcggatcaa cgaatcgaaa
     7621 aaaataattc agtgatgcat cgaaagaaat actatgaaaa agttcatatt cattgatttc
     7681 taattctaat acattcgctg tcagaagccg gcggtatttt tccggtcctt cgatctcgta
     7741 tccctggttc cgaatgattt ccaaaggcag ttggcgaata ctttcttcca actgtttgat
     7801 atctgcataa aaagtggtat tactgatgcg atatgattcc atgaaatgag caagactcaa
     7861 cggctctgtc gtcagtaaca actcggctaa aatcgcgtgt tgtcgtttag cagtggataa
     7921 gtcattactt ttcccatctg tattttcttt taattgctct aatacttctt ctgatgcctc
     7981 aatgacaaat gagcctctag ctgctttttt caaagaagca tttaccgatt tcaatgagac
     8041 ttcaagatta tctaattcgc ggtaaatagt ccgtttactg accttcaata aattcatcat
     8101 tttttggata gatacaggtg acgggctact gagaaattct gctaataatt gtttttctct
     8161 gggcgataaa tacatcttat atttctccct tcacgagttt ttcgtattca ttcgtagcta
     8221 cccaatgttc aactagaact agccgaggga tcttttcttc tagctgttca gttacagcta
     8281 gttctctttg tgcgatataa atcgtttctt cttggatttt gattttttct attgattctt
     8341 ttgagataac aaactgtttt ttcgcttttt ttgctaattg acttaaaatc gtcatgccca
     8401 tagtttgtga gccgcgtctg ttgtttttat acaaaaatac aatattagca ggcgactttt
     8461 tttgttctgt gatatctgct atctcttttt tttcttcccg ctttggaaat tggatcgctt
     8521 cttttattcg tgacagcaag atatctgttt ccaaaaagtg atccagtaca tagatttgac
     8581 tttgaggtac tttttctctt gcaagacccg ctagttcatg ttgcacgata accagctgat
     8641 ttacttggtt attcaattca tagatagagc tgtatccgac agaaagatcg attttttctg
     8701 ctgctaattt ctttttcagc agggcggctc ccatagcact tgatcccata cctgagtcgc
     8761 aagcaaaaat gacggaatga atgatagatg gttccaagat ttctcccctt tctttgaccg
     8821 ttttgttctc tttttcttta tttggtaatt tcaatagtat cgctgcacaa atgaaggtca
     8881 caaatgtact gattgctatc cctatactta ccccagctag ttgtggataa ggcgcattgg
     8941 caaaaatcac gagtagtgac ccaggcgata caggtgcagc taatcccgta tctaaccatt
     9001 gaaatatcaa cgtgccgctt gccccaccag ccattacagc taaaaataga tacggtcgca
     9061 gcaagacaaa tggaaaatag atttcgtgaa tcccgccaat cgcatggatc atcaatgctc
     9121 cactcgcatt tactttttcc tgtttttctg caaaacaaag gatagaaagt aagatcccta
     9181 aaccaggtcc aggatttgcc tccatcaaaa ataaggaaga gtacccgact gtttgtgaga
     9241 attcgattcc taaaggtgtc aatacaccat gattgattgc attattgaaa aatagtactt
     9301 ttagaggttc taagatcaga ctgacaagtg gtaacagatt cattgtgact agccaagaca
     9361 caacggaggc gagcaacaag tgaacactat ttaaaactgg ttcaataaaa acgattccaa
     9421 ttatacaaat aacgctacca gtcagtccta ctagtaggtt gctgacaagc atttcatatc
     9481 cagccttgaa tttcacgtgg aatctagaaa caaattgttc atatacgatg ccggcaagcg
     9541 gcccgataat catcgctcct acgatttgtg gagcattgga acgagcgatc aaccctaaag
     9601 aagcaattgc accggcgact gccccttttt gtccccctac cgatttcccg cctgaataac
     9661 tgatcaagat gggtaaaagc agctggatca tccaatggca tacctcttct aatgtggtgt
     9721 gcaaatcgcc agacacacct tttgttgcta atgacaagat tccccaagcg ataaacagac
     9781 ttagatttgg catgatcatt tcatttaagt gatgtccagc ttttctcagt tgtttcatag
     9841 acacgcgact gctgttgttt gctttccatt tgtattccat ctttttcacc atacttctat
     9901 ttattgatgg acggatttta tgtagaaaaa gcagcaaacg aaaaaccgtc tgctgctttc
     9961 agctatctgg gaactatttg cctggttcag tcgcaatgat tgctagttca ccttggaccg
    10021 cccactcaga aacgccattt ggaataatac agcttgttcc tttttctaat ggatatgttt
    10081 ttccgtcaac gacaaggtct cctgctcctt cgattaccgt catcaatgta taaggggctt
    10141 gttttttgaa agatgtgatt cctttaatat cccactcata tacattgaaa aactcagttt
    10201 ctaaataagt gacgatcgat gaattccctt ttcggacttc tttgatttgt aattctggtg
    10261 ttttcgcagg gacagttgtt acatcgatcg attgttggat atgcagctct cttgtttgtc
    10321 cttgcgcatc ttttctgtcg tagtcataca cgcggtaagt cgtgtcagaa ctttgctgtg
    10381 tttctaaaat catgattcct ttaccaatag catggatcgt tccgcttggt acataaaaga
    10441 aatcaccttt tttaactggt actttcttca aaaggtcatc ccagcgccca tctttgatca
    10501 tctcagctag ttcttctctt gtctttgcat gatgaccata gatgatttct gcaccaggtt
    10561 cagcatcgat gatataccag cattcagttt ttcctaattc accttcatgt ttcatcccat
    10621 aggcatcatc tggatgtact tgtacagata gatcatcttc tgcgtctaag atcttgatca
    10681 ataatggaaa cactggttct gaaggatggc caaacaattc cttgtgttct gaccatagtt
    10741 catctaatct tttccctttg aattcgccat tttctactac actgacacca tgaggatgcg
    10801 cactgatcgc ccaatcttct ccgattttat cgttagggat atcaaaacca aagactgaac
    10861 gtaaacgact tcctccccaa attttttctt gaaaaactgg ttttaaaaac ataggttcct
    10921 gcataattca aactcctctc atttttcata gctattatag cattatttcg gtaataccgc
    10981 tttctctttc aaaaagtttt caacaatttc atctcgacat atgagatact ccgcacgatg
    11041 atcgacagga tgtacgcgat ttgacaaaaa tatcaacgct tcttgtgttt ttatatcaat
    11101 caagaaaaac gtacctgtat aaccagtatg aaaaagaatc gcatgaccgt cttctacatc
    11161 cttttttaag tcccatccta aagaacgata gccatttttt ttcggtgttt gatcttgcag
    11221 taaagaatca atcgtctctt cttctaaaaa acgaccatta cggatttttc ccttgttcaa
    11281 atacatttgg acaaaagtaa tgctgtcttt caaatcaata aagagacccg cgttacctgc
    11341 atgttcagcc aaaactcttg ctttcggatc atgagtttgt ccacgcaaaa cctcacccga
    11401 aggcagttgt tctgtgggaa cgattttttt ggaaggaaaa ggaagaaata agctgttcat
    11461 cattcctaat ggttccaata cttgttcttg aaagatttcc actgcagttt cttggtaaat
    11521 ttcctctagc atcaacccaa gaagaatcgt accggcatct gtatatttta cttcttttcc
    11581 tagttgctca cctggttcta gcttcaaata agcggcgatc aattcttcct gagatagctt
    11641 atctctgttt ggtatccatg ttttgatatc agaagtatgt gtcagtaaat gacgcaaagt
    11701 gattgtctga ttttcaaatt caggaagata tttatgaagc ggttgatcga gttctatctt
    11761 tccatcttct aatagacgta aaactaatga agtggttgca acgactttcg tcagtgaggc
    11821 tgcatcaaac aacagatctt ctgtcatcag ctgcttttca ggaacgactt gggcaaagcc
    11881 ggctgtaaat gtttctactt gattttcttt tatgaaggcg actgctacac ctggataaaa
    11941 tccttcttcc atctttttca aaataagctg tttcgtgtgt ggatacatct ttttctcctg
    12001 cttttctatc agattcttac agtccaagct taaagcttaa tctatgagga atcaaccttt
    12061 tttacacaaa aaaactgcgc gaaaaaccgc gcagtttccc ccggtatact attttctctt
    12121 tctatctcta gaataagcga tagtatgacc gttcataaaa tgacgagaca tgcgacaatc
    12181 gttaagaaag aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt tcttttggct
    12241 cactgacgaa ccaccattcg attcctatag catcatagat tgtgagtact gtgtgctttt
    12301 tgtctaagaa gaaatcaact gatagttgct gcaagtgagc aatcagtcct tccatgcttt
    12361 cagtatcgat catgaatttc agaaaatcaa gtccaatcac ttcatctgta ggcaaatcaa
    12421 ttgcgcctcc gctagcttct gtcaaaccaa catggaaatc tccttcattc aatacatgga
    12481 tgccctcttc ttcattatgt accttcaaac caagtacatg attaagaaat tggcgttctt
    12541 ttgttaagtt agtaacattt aaatggattt tttcaaaata agaacctgtt gatagtttac
    12601 tgaatttttc ttcagatttt cttaataatg cttcttgatc cattgtttct ggaatttctc
    12661 tttggtcgtt cttattctct tcatggtaaa tttccaattc attctcttct gggtctacga
    12721 gcaaaatccc catacgtcca tgatcttcta aagcttcttt gattggataa ttcattttac
    12781 ggatacgaca aaggatatct gccatttctt ctgtgctagg gataatcaat gaaaaacggt
    12841 tcagcttttt gatcaccccc atgtgatcat tcgctctagg actttcttcc agcagcaaca
    12901 tttcacttcc agcttcttct gttcctaaaa tggctaattc gttttcttct cttttcaaat
    12961 caaagccaac aacgttcttg taaaaatcaa tcattttatc tcggtctttc acccgaatgg
    13021 ctacactttt taaataggtg gcagccccca attgaaaatc tttcatattt cttgctcccc
    13081 tctacgtcaa gctttatttt aacgatggat tcttcttctt tcaagcaata actttgattt
    13141 tgttaaaaaa aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt aatgagtaaa
    13201 aactcttata aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    13261 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
    13321 tgaacaccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta ttagagatac
    13381 aaaacggaaa aatagcttct catattttgc cgttttttga gctaataccg aaggacagct
    13441 gattgaacac cgtttattcg gaaaaaggga ctgtgacacg acttttgtca cagcccctaa
    13501 aaaaaataat aaatttgtac tattctatga agcttcttct tcagaaaact gactgttata
    13561 aagatctgca tagaatccgt ctttttccat caatgtatcg tggtttcctg tttctacgat
    13621 cgaaccatga ttcatcacaa taatgttgtc tgcatcgcgt attgtagaca agcggtgagc
    13681 aacaacgaaa cttgtccggt tttccaatag gcggttcatc gctttttgaa tcagtatttc
    13741 tgttcgtgta tctacactag atgttgcttc atcaaggatc aggacatctg gatttgctaa
    13801 gaatgcccgc gcaattgtga ttagctgacg ctgcccttgc gaaatattgc ttgcatcctc
    13861 gtttaataca gtgttatatc cttctggcaa tttacgaaca aaatcgtcta cgtgagcagc
    13921 tttagcagcc tcaatgattt gttcgtctgt tgcatcgtcg ttcccatatt tgatattgtc
    13981 atagatactt cctgtaaaca gccaagtatc ttgcagtacc attgaaaact gtgcgcgcaa
    14041 ttcatcacgt gaaagatcac gggtatctac accatcgtaa cgaatactac ctccagaaac
    14101 atcgtagaac cgttcaagaa ggttgatcag tgtcgtcttc cctgcaccag tcggacctac
    14161 aatagcgacc atctcaccag gtttgacatc caagttaaaa tcagtcatta gcaagttatc
    14221 tggtgtgtag ccgaattgaa cattttcaaa acgaactttg tatggtgtat ctttttcttc
    14281 tggaaggcca gatttttcat tggacatctc ttcttcatcc aacacttcaa atacccgttc
    14341 ggctgaagca actgttgatt ggatagtgtt catcaaactt gcaagttgag tgattggttg
    14401 agagaactgt gttgtatatt gcaagaacgc ttgaacatcc cctaatgtca tgttcccatt
    14461 tgcaactttt actccgccta gtacagcgac gaacacatag cctagatttt taacgaaatt
    14521 catcaacggc atgatgattg ccgaaataaa ttgagctttc caaccagcgg catacagatt
    14581 ctgattttct ttttcaaata cttcctggtc tttttcagta tgattgaatg ttttaacaat
    14641 ggtatgacca ccatatgttt cttctacttg gttattcaat aaacctaaac ttttttgctg
    14701 atctgcgaaa aacacttgtg atttcggcgc tacgatcatc acgatgatca agcttaaagg
    14761 gatcatcaat aacgcgacta acgtcaattg ccagctgatt gaaagcatca tcacgatgac
    14821 cccaatcaat gtgactgcac tagttacaaa ttgagtcaaa ttttgctgca gcgtgcttgc
    14881 gatgttatcc atatcattga ttgctcgaga catgatatcc ccatttgtat gtgtatcata
    14941 ataggaaatc ggtacacgat tcattttggc ttccaaatca tgacgtaatt cataaactgt
    15001 tcgttgggaa acacgtgtca taataaattg ctgtaaaaag ttgaatacag cagaaatcaa
    15061 atacatcacg attactgtcg caatgatctg cgcgatttta tcaaagtcta tcggaaattt
    15121 tgttacttgt tggccttgtt gcatctgttg agcgccagcc attacacctt tgaatatttc
    15181 tgtggttgct ttccccaaga cctttggtgt ttggatttgg aagatcacgg ctgctattgc
    15241 tagtacaaat acagcgatga tcgcaaccat acgtttagac atatagccaa acaatcggcg
    15301 taccgttccc cagaaatttt taggcttttc accttttaca ccgagatttc tgccaggtcc
    15361 accatgtcct ggaccgcctg ctggtccatg agagggacgt gtgtttgttt tactcatgct
    15421 aagtcctcct ctcttagttg tgaatgaacg atttcttgat aagtttcgtt tgttgctaat
    15481 agttcttcat gtgttccttt gccaaccagt tttccttcat ctagcacaag gatcatatct
    15541 gcatccatta ctgtacttac tcgttgagca actaaaacgg tgatactttc tttcatattc
    15601 gttttcaatg cttggcgaag attggcatcc gttttgaaat ccaacgctga gaaggaatca
    15661 tcaaacacat agatatctgc tgttttgact aatgcccgtg caattgccag acgctgtctt
    15721 tgtccaccag agaagtttcc tcctccttgt tccacatggc tatctaagcc atcttccatc
    15781 tctgaaacaa aatctttagc ttgtgcgatt tctaatgctt tccaaatcat ttcatctgtt
    15841 gcatccggct taccatactg catattgtct cggatcgttc cagagaacag aactgctttt
    15901 tgaggaacaa agcccattaa ttctcgcaaa ttatattgag tcatgtcact aatgtccgtt
    15961 ccatttattt ggatcgaacc agattcgatg tcatataatc gagggatcaa attgactaag
    16021 gtcgttttac ctgaaccagt cccccctatg attgcgacga tctcgcccga cttggcttgg
    16081 aaatcaatat cttccaatgc aagtttttct gctccatgat atcgataatt gacatgattg
    16141 aaggccaatg ttgcgttttt tccagcaaaa gaaactgttt taggattttc agggtcatgg
    16201 ataccgattt tttcatccaa cacttcatta atacggtcag ctgaagcttg tgcacgaggc
    16261 accatgataa aaatagcgga aagcatcata aagctgatca agatctgcat cgcataagtc
    16321 ataaatgcaa tcagattacc gacttccaaa gtcatatctg ctatgtagtg tccaccaaac
    16381 caagtgattg ctacgtttgt accgctcatg atcaatgtca tcattggcat catcaatgcg
    16441 acgatcgtat taactttgat tgccgtattt gtataatctt tattcgctaa atcaaaacga
    16501 ttttcttcaa agcgtgtctt atcgaatgct cgaatcactc tgacccctgt caatccttct
    16561 cgaaatacca ggtttaaacg gtcagttttc ttttgcatac ttttgaataa tggaacagca
    16621 aagtacatga ttccgccgat caaaataatc attactggga tgacaactaa gaagattttt
    16681 gtcaactgat gatctttttg ataagcaagt atgctcgcgc caatcaacgt gattggtgca
    16741 ttgatcatca tacgtaaaaa catttgtgta accatttgga tttgagtcac atcattggtc
    16801 gttcttgtga tcaaagaggc cgttccatat ttatcaaacg catcttttgt cagactctct
    16861 gattttttat agacatccgt acgcaattgc ttacccagtt tctgtgattc acgagtagca
    16921 aaaaatgtat ttgcgatagc agctaagata ctgatcaaag aaaagccgat catcacaaaa
    16981 cctgtatgcc aaatataatc gacatctcct tgcgctaccc ctttatcaat aatattggag
    17041 gtaagtgtcg gcaaatataa atctgccagt acttggatca ccatgaaaac tgcagctgca
    17101 attgctgaag tcaaggagat ccgttttacg atttttagca tctagttccc ttctttctat
    17161 ttatatactc cgtttttcag ccaagaaagt ttcaaattga attcactgat tgcttgctct
    17221 actgagtaat cttcagatgt tgaaaataag cgatacgaat gcgcaattgt tgtaaataca
    17281 gtattcatca ctaattggat cagtaattcc aattcatgat cattttttac tttcagcttc
    17341 gtccgatcga tcaattcaag caactctttt gcatcttgtt tgtgttcttt atgtggatcg
    17401 tgaggcgttt tttggcgact cgtatgagga gcgacttttc ttgaggcacg gtaatccatg
    17461 ttcataaaca agttacgata aaaagaagca ttctcgcctt ccaaaatctc ataaatcatt
    17521 ttagaaaaat aggattcgaa tcctttgaat agatcaccat cggattcttt cattagttgt
    17581 aacatatctt tagagctgtc acgtctcatt gtctcaaagt aataaaaata aagatcctct
    17641 ttatcttcaa aatactgata gaaactgcct ctagggatat ccgctaattt gacgatatta
    17701 gcaatcgaag cttcttttaa gggtactcta gaaaactcga ttctcgcagc ctcgattagt
    17761 cttttttgtt tttctttagg caaatggaaa aatgtctgtg tcggcatatg gatcctcctt
    17821 tctttcaaga tgacacattg tcatatgacg ctgtgtcatt ataacacctc ctacctgttt
    17881 tgcaagacat ttgcatagtt tttttcatta tcatccgata cattaaaaaa gtgataagag
    17941 gaatccttcc atttgaatta atgagagttg cccattaaaa tagccacata ctaaacaaag
    18001 gatgagataa atggcccaat caattagtgt ccaagacttc aataaattgc ctctcgatgc
    18061 tgacagcatc gttttagata caagaaatac cttagattac acaaaaaacc acttaagcca
    18121 ctcgctttct atccccgctg ctatcttgcc aagacaatta aaaaatctag atccaaagaa
    18181 actttattat attctaagct atacaggcag gcgatcagaa gcattagccg atcaacttac
    18241 tgctaatggt tttcgtgctg tttatatcat tggtgggatg gaagctcttc agtcgagtgt
    18301 tgcttgaata ttgcttgtta gattcaaaag aggctgtgac aaaaaatcgt gtcacagcct
    18361 cttttatacc ctgtcttttt ttagttattt attcttcaac cttatcaata tgacttgata
    18421 gccttttctt tttctcatag ttataacaat catagcaata aaaggagaag gtaagcatat
    18481 ggcatctgtt ttagtagtca aaggtcatcc cctaactgca gaagaatcgc gcacagttaa
    18541 ggcattgacg tcttttttaa caagttataa agaaaatcat ccagatgatg aagtgactgt
    18601 tttggaactt tatcgcgatg atattccgga aatcgatgaa gaattattat caggttggga
    18661 agcattacgt gctggtgcag aattcacttc cttatcagaa agtcaacaac aaaaaattgc
    18721 tcgtttcaat gaattgactg atcagttcct tgctgctgat aaaatcgtta tcgcaaacgc
    18781 cttatggaat ctaaatatcc caacaaaatt aaaagcttgg ttcgataccg tcaatgtagc
    18841 tggaaaaacg ttccgctata cagaaaacgg gccagaagga ttagtgacag gtaaaaaagc
    18901 actgcacatc caatctaatg gcggtgtata caacggacaa gactttgcct ctcaatatgt
    18961 aaaaggtatt ttaaactttg taggcatcga tcaagtagat cagctattca tcgaaggaat
    19021 cgattatgac cctgaccgtg cagatgagtt gatgcaaaat gcattagata aagctgctgc
    19081 tttaggtaaa tcattttaaa aattgagata aatgcataca aaagagacag aggaaaaaat
    19141 cttcaaagca gcatttgtac cttggatatt ttcccatctg tctctttttg ttattctttc
    19201 tgctttttca acggattttc ttcataaaga gaagagattg ttgtgtacca ttcaaaaaca
    19261 tgagtaatca ctactttagc tgctgcataa acaggaatcc ccaagatcac accgatgatt
    19321 ccgaacaatt tcccagaagt cagcagaact aataaaatcg ttactggatg gattgctaac
    19381 tgattcccta gtactaatgg agagatcaag cgtccttcta ttgtctgctc tactgcaaaa
    19441 acgatcagta ctttgatgat catgaatgga cctgctacaa tggctaaaaa gatagccgga
    19501 atcatcgcta aaaatgatcc taagtaaggg atcaggttca aaaaacccgc aataatcccc
    19561 aacgtgacag catactctaa accaatgacc gcaaaaccaa tcataaacat gattgccaca
    19621 gcaaaagcca cagttagttg tcctcttata taagaagaaa cttgactatt catttcactc
    19681 agcacattca acgtcggttt ttgcatgcgt gtcggtaaaa atttgacaaa ataaggtgct
    19741 aattgtttac cgtctttcaa tagataaaat aagataaacg gcatcgtcag cagagccact
    19801 aaaatagtcg ctaccgcgcc gacaaaactt ccaagactct gaactgtact cttagatata
    19861 tcttggatca tatcgcctgc cgaattcatg aacttatcac ctgctgtttc caactgttcg
    19921 cgaaattggt tgaacagcgg gtcccgcaaa atctcggtta atttattatc aatcgtatct
    19981 acatattgag ggaagttact gatgaagctc actgtttgtt cctgaatctt agggatgatc
    20041 actacgctcc cccatgcgat tagtgcaaca accaaaacaa acagcccaat gatactgtaa
    20101 agccttggta cttttcgctt ttcaaagtaa tcaactaccg gattcatcaa gtaatacaag
    20161 attcctgcta aaatgatagg cagacctacg attgctaaaa attgccagat tggtgcaaac
    20221 aaatatgaaa cttttgtaaa taaaaagacg atcagcaata ttaacaatac aacaagtaag
    20281 gctgtgacta cttgattatt taaaaaccag cgccaaaacc acgataccgg tctgttcttc
    20341 ttattttttc cttccataat tcctcctgtt agttataaaa aatttggctt cccaaaccaa
    20401 tagcaggcag tctatgcgga tgaagataga ttccatttgc aatttgtcct tcttctggac
    20461 aagcatcaaa aatcaatgat acgtgagcta ctgtctctaa attcccattg gccatcggac
    20521 cgacataatc gatcgtatat acctgatcat caaaagcaat tgttccgcct gctttcaaag
    20581 aaaaaggttc gtcttttgat acttcttgaa tcaccgaata ttttcgtaag gcagctgttg
    20641 ctgttttatc gaacaaaatc agcatgggtt cttccttgtt tattgcttct gtcccaattt
    20701 ctgtaatctt tgcctgaatc attgcactct ctcctttaat tgctttcaaa aacagtataa
    20761 cacattctca ttctttagcc gtgtggaata attatgtgat agaataaaaa cgaaaactat
    20821 ttcattacaa ttaggaggaa tcagctcata tgatcgaaaa gataatttta ggaacctata
    20881 cgcgtcgcga aagtaaagga atctatacga tcgaccttga tacagaaaaa gaagaattag
    20941 ccaacctttc attagctgtt gaagaaaata gccctaccta tgtagcaaaa agtaaagctg
    21001 gcaatcttta cacagttact agtgtggatg gattaggtgg tgccggtgcg tacgataagg
    21061 attttcattt tttgaatgcg gtaacagagt ctggcgctcc tttgtgttat gtagcggttg
    21121 atgaagcacg tcaattacta tacggggcga actatcataa aggtgaagta aatgtctacc
    21181 atatattagg agatggcgaa ctgaaagcag cagattcaat attccatcaa gaagcgacag
    21241 gtcctcataa aaaccaagat catgcgcatg tccactatac ggatctgact cctgaccaac
    21301 gattagtcgt atgtgatctt ggtacagatc gtgtgtatac ttatgatgtg tccgaaaacg
    21361 gcaaattgaa tcttgtaact accttcactg cagagccagg aacaggccct cgccatcttg
    21421 tctttcatcc aaatggacaa ttcgcttatt tatttggtga attagacagc acagtccgcg
    21481 tgctttctta taataaagaa acaggtgctt ttgaagaatt acagaaaata tcgaccttgc
    21541 cagaagaatt cgacggtgaa aatggagggg cagctatccg catttcaaat gatggcaagt
    21601 tcctttatgc ttctaaccga ggacataact cgattgctgt attcgcagtt tttgaagatg
    21661 gttcccacat tcaaaacaag caaatcatct caacagaagg ggattttccc cgcgatttcg
    21721 cactagatcc aagcaataat tttgtcgtgt gtgccaatca gaacaccgat aatctgacat
    21781 tatatcgccg tcatcctgaa acaggattac tggaattact gaaaaaagat gtctatgtac
    21841 cagaatgtgt ctgtgtttat tttgatcgtt agtaagaaaa ggagaatgaa atgaatcagc
    21901 aagacgtatt agacagacta cgggaagaac taaatattcc tttctttgat gggaaaatcg
    21961 aagataaaga gtatagcgaa gaggaatatc agaaactcaa agccgattta cagaactact
    22021 ttgaacaata tgtacagaat gtagaaaact aatcagcttt ccatactatc tgaaaagaac
    22081 atcaggacaa gacttttccg tctatcctga tgttcttttc tacaaataaa aaaccgccga
    22141 gatggttaga ctcgacggaa aaggagtatt tactcgattt tgagtaagta caaggttgtt
    22201 gttggttata ggtatgtcat tatcataaag gattttgatc actctatcat aaaggtaata
    22261 ttacatttat attagataac tctagttttt attgttatca aagatttctt ttattttttc
    22321 aacaacaaat tagtcatttt ttggatggca g
//
LOCUS       DACDUU010000049        22026 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.049, whole genome shotgun sequence.
ACCESSION   DACDUU010000049 DACDUU010000000
VERSION     DACDUU010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.049"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(61..1371)
                     /locus_tag="IWL30_001838"
     CDS             complement(61..1371)
                     /locus_tag="IWL30_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP9706592.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1383..2801)
                     /locus_tag="IWL30_001839"
     CDS             complement(1383..2801)
                     /locus_tag="IWL30_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9706593.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3041..4609)
                     /locus_tag="IWL30_001840"
     CDS             complement(3041..4609)
                     /locus_tag="IWL30_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAP9706594.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4613..4894)
                     /locus_tag="IWL30_001841"
     CDS             complement(4613..4894)
                     /locus_tag="IWL30_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP9706595.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(4891..5814)
                     /locus_tag="IWL30_001842"
     CDS             complement(4891..5814)
                     /locus_tag="IWL30_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAP9706596.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5811..6689)
                     /locus_tag="IWL30_001843"
     CDS             complement(5811..6689)
                     /locus_tag="IWL30_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP9706597.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6676..8013)
                     /locus_tag="IWL30_001844"
     CDS             complement(6676..8013)
                     /locus_tag="IWL30_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP9706598.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8016..9119)
                     /locus_tag="IWL30_001845"
     CDS             complement(8016..9119)
                     /locus_tag="IWL30_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP9706599.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9193..10701)
                     /locus_tag="IWL30_001846"
     CDS             complement(9193..10701)
                     /locus_tag="IWL30_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9706600.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10667..11359)
                     /locus_tag="IWL30_001847"
     CDS             complement(10667..11359)
                     /locus_tag="IWL30_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9706601.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11381..12355)
                     /locus_tag="IWL30_001848"
     CDS             complement(11381..12355)
                     /locus_tag="IWL30_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9706602.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12655..13527
                     /locus_tag="IWL30_001849"
     CDS             12655..13527
                     /locus_tag="IWL30_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9706603.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14109..16307)
                     /locus_tag="IWL30_001850"
     CDS             complement(14109..16307)
                     /locus_tag="IWL30_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9706604.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16539..17531
                     /locus_tag="IWL30_001851"
     CDS             16539..17531
                     /locus_tag="IWL30_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9706605.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17591..19012
                     /locus_tag="IWL30_001852"
     CDS             17591..19012
                     /locus_tag="IWL30_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAP9706606.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19005..20504
                     /locus_tag="IWL30_001853"
     CDS             19005..20504
                     /locus_tag="IWL30_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAP9706607.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20543..21937
                     /gene="uxaC"
                     /locus_tag="IWL30_001854"
     CDS             20543..21937
                     /gene="uxaC"
                     /locus_tag="IWL30_001854"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP9706608.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         6669 a         4494 c         3823 g         7040 t
ORIGIN      
        1 atcattaaaa aaaataaagg tcaagctaaa atattattta agcttgacct tattataatt
       61 tcaacgattg tcaaaagtat tgtttgcagc tgtgttgcca gtttctatag ataaatcaca
      121 gcttttgaaa cataagtttt gtactgcttc gattcgcccg aattcttttg cagttatttg
      181 gcaatgagaa aagcagacat cttctatagg cttttcagga aatgctacta aatcaaaagc
      241 tcgggaagga agtgagtaac ctggagaaag gcatgcctgt acgtttttga tgtgtatgtc
      301 tttgactttc gtcatttgct ttgattcagg tatttcttca gccactgctt gccaactttc
      361 tggcatttga gcaaggtcat tgctattttt tctattgtat tcattatgcc agtccattat
      421 ccaagaaaaa ggaaattgta catcaatcat attcagattt tctaccctga tattttcgat
      481 cactccccct ctttcttttg aagatttcat tcgaatacca cagtctgact gaaaaaaatt
      541 gatatcgtga atatatacgt cactaactcc agcactaact tcgctaccaa tagttacacc
      601 gtatcctgaa cggattttac atctagcaac ctcgattctt gatgattttc gattgactct
      661 tgcaccattg ccatctctgc cagacttaat agcaatacaa tcatcaccac aggccaaatc
      721 acattcatag acacgcacat ttgttgaaga atcaatatca atcccatctg tacttggtcc
      781 atcattgttg cggatgatta ctttttctac taatacatcg ttagaataag tgatttgcaa
      841 gttccaaaaa cctgatttct gaaaaatcaa atcacgaaca acaacatgtt ctgcatgata
      901 cagtaaacaa gcgcgtggac gtttaatagc ataatccgca atccaacgaa gtcccttttg
      961 atcgtattca gctcttgtcc cgcttttttg atcttttccc caatacagct cccaccaatg
     1021 gtctccttga ccgtcgatta ttccttttcc ttctataaga atatctttcg ccgagagaac
     1081 attcaaaatc gctgctggcc atttcatttc tacgccggct actcgtgtat cgatttctgg
     1141 aaatgcttcg atatctgttc tccccttgag gactgcacct tcttcaaaga taagatgact
     1201 cttatctttt aaaaaaaggg ctccgactaa ataaatgcct tttggaataa atacaggtaa
     1261 gtgttctttc gcaccttgat cgattgcttt ttgcagtatt attgtatcat cttgttgagt
     1321 gtcgtttgtt ttaaagttca aagcattgat aatccctttt tttgtccgca atacgtttct
     1381 ccctatcttt cattgatttt tttcaattct tcaccaataa atacagtttt tgaacgattc
     1441 agtgggtaac ctactagcaa aatgattgcg gatacaaata agcagaatgc tggtaacaag
     1501 tttgccatcg tataaatcct ttcttgtact aaaactgatt ggatggctcc acctgttgta
     1561 gacgattggt aaccaatgag tgccagcata aatccgccta ccgctcctgc acatgcttgt
     1621 cctacttttc tcgcaaaaga atttactcca tatactgttc catcttctct taagcctgtc
     1681 ttatattgat gataatcaat cacgtcagta atgaatgccc agaccatcaa attgaataaa
     1741 ccagatccta aagtagcgat aaataaaaga atcagatata cccatgcatt tgttatatga
     1801 aggaaataca tcagcaagta catacatgaa gaagcaagta acgcaaatac gcttgcttcc
     1861 tttttaccaa ataattttgt cagctttgta gcaaatggtg ctaaaactac tactgttccg
     1921 taagtaaata aaagagcaac agacattgct tgtttattat ggaagtaatc attgaataaa
     1981 taagtcatat tcgtcccagc taaaatctga ttgatcacaa tgaaaatgtc aacaatgact
     2041 aaaataatca gtgctcgatt agctaacaaa ccttttacca actgagaagc agatactttt
     2101 tcgtttttga caactcttac tctttcagta gtcaatttac aagttaacat atatgcgaca
     2161 aatgctattg cagcacatcc gattgaaatc caaaagaaat gattccctga aacgacttgt
     2221 tgtccactct tgttatgggt atacattaaa atcggaatga agaatcctgt agttgatccg
     2281 ccaatcgcag aaccaatgct tcggaacgtg gacaacgagg tgcggtcatt aggatttgag
     2341 ctaattgctg ctgccattgc tccatacggg atattgattg ttgagagaaa aatcccataa
     2401 acaatgtatg aaacaaaaat ataagcaatc ttcaccgtca ttggaagatg ttggacgaat
     2461 ggcaaaaaca aaatgacagt cactaagcaa aatggatact tcatttttct gatccaagga
     2521 ataaatcggc cagtttctgt taactctgag acatcacata atcttccaac catcacatca
     2581 gcaaacgcat ccaaaattct cgaaatcaag aataacgtac ctacgattgc ccctgaaacg
     2641 ccaagaacat tcgtataata aatcatcaag aaactattga ctaaacctaa aataaaacag
     2701 ttacctagat caccgaacat gtagcctatc ttatcggaaa tcccaaattc ctttttttct
     2761 ttttttactg tatttgctgt aacagcattt tgtgaaagca tttgaattcc tcctacctct
     2821 tctagtaaac gttttcaaaa ttataactct ttttttcttg tttttatatt cacaatgttg
     2881 gctttttatt gcattatctt gacagataaa aaacaaaaaa taatctaaaa acattgatgc
     2941 ataagattga taactttttt tacataacaa tatatacaaa caaaaaaatt cactaactag
     3001 tgaaaataag ttagtgaacc tcatttatta aagaattaaa ttacagatga tcttttccat
     3061 catacttgaa aaactcaaaa tcagcataac ttcctctttt tttatttaga tcatgcactg
     3121 ctatcccgat aaaatttcca gtaaaccctc ctgagagtat acacatgttt ttttgcggac
     3181 ctactttctt ccattcttca gttgatacgt ctttcaaatg aaactgtgct gtctctttct
     3241 ttacagtgac tttcaaaaaa gtcttctttt ctgtgagtgg aatcgtccaa tcttctattt
     3301 gtgctactcc gttttctgat tctatcatac gtaggcattt tcccttgtcc tcattccaag
     3361 taacataaca ataaatataa ttggattcat ttaagtacag taccagtcca gccatctgat
     3421 taaaattatt aggcatgtat tcaaatgctg tctccgcatc aaaagtaaaa tccgtttgcc
     3481 gaatagctaa caaatgatgt tcgaaggtag attgaagaga ttcaccggaa atcagacgta
     3541 aaaaacctgg tctacatttt gtatcacacc aatcagcatt tggcaaaatg cgtctggcat
     3601 tccagttctt ctttagctct ccttcaaatg tatccataaa ctgatgatct ttttcttgtt
     3661 gtacctcttc atttgtctca atcattattt cttccatcgg agttgtctgg ctatctttaa
     3721 gtcttaacca atcgtcggac gtccaataca cttcttgaat agcggtttct cttcccaaaa
     3781 tagccgcacc gtttaatggt ctggtacaaa gatatgtcat gtaccatctt cctgacgggg
     3841 tttgtactaa gctcccatgt cctgtacatt gcaatgtaga agcaggtttg tcactagctg
     3901 tcagcatagg aaaaccagga tcaagctcgt aaggaccagt aatattcttt gctcggcaaa
     3961 ctgtaactga atgtcctctt cctgtaccgc cttctgccgt catcaaataa taatacccgt
     4021 tacgaaaata caaatgtggt gcctccgttt tcgctaattc tgtacctgca aatattttat
     4081 agatgggacc atcaagcttc ttcttctctg catcatattc ttgtaatacg ataccgctgg
     4141 atttattcgg tgtgtccaga cgataatccc atagttcatt gacaagccat ttttttcccg
     4201 tttcaagatc atggaacaaa gaaggatcaa agccgttact atttaaataa attggttcgg
     4261 accaaggacc tgtgatctca gatgcagtga tcaaataatt gtggacatct ttaaacggac
     4321 ggcctgcact tttgacatct gtgtaaacaa gataaaacaa tccatcagca taactgatct
     4381 gtggcgccca tatacttcca tttgtaggat ttccttgcaa agatacttga gtggtcaaaa
     4441 tatccgttgt atgtgaccag ttgactaaat ttttcgactc atatactcgg attcctggaa
     4501 gccattcaaa agaggagaca ataatgtaat accgctcttt cactcttaat atattaggat
     4561 ctggattgaa tcctcttaaa ataggatttt tcactttgat agtctgcatg attcaggctc
     4621 cttttctcat tcttactcga tataaactga atgccccagc tgctaaaaaa gcaagtgaaa
     4681 taaacaacac aacggcaaac agaccagtca tgtcctgtaa taatccaaat gtattttcta
     4741 aagacaaagt tgaatgatct acagcaagcg actgataact ttttttaaac tctgacgcag
     4801 acatattact taaagtaact gctaacccgc caaagaaaat caaagataga ctgccaccga
     4861 ccattcctag caatagttcc cattttcttt tcatttctga accccctgct tttctaaatg
     4921 tatccattca ggtcttttga tcgatgtttc ataattgtta ttacgttcag agaatttgac
     4981 agtctgttca tttttcttgt catcccaatt atcccaacca gttgggtgaa catgcccacc
     5041 tatcttacac gcttcaaata agacatttgc atacggacgc cacggtcttc ctaaatagac
     5101 gtttctagta tcaggttctg cagtcaaggt acagctttca aaacgatacc ctgttttttg
     5161 atttttcggc gtagatgctg ctgtgatgta tccaggattt ttactggcta agctatggat
     5221 atgacactga tgaaaaacgg catctgcccc accaaatatg aaatccaccg ttccttcaat
     5281 ccagcaatat tcataggtct gacgacaata ctcaggattg gcagaagccg gtgtacaaaa
     5341 aggagtacca tctttttgca aatcaggcaa cggacccgtg cataaggtat cctgataacc
     5401 agatagtcga cagtttttta aagatacgcg atctcctgaa ttgaataaag caacagcctg
     5461 tcctaccttt tctcctggac ctgctgtatt ttcgatgtga agattttcta atacgacatt
     5521 cgatccttct aagaaaaaag tagcacttcg gaaagtccct aactggctac cgtcaagata
     5581 ctcctgtttt gcaaaacgat taccggagat aactactgtt cctgctccca caagtttaaa
     5641 attggataat ctgctttcta tatattcatc atatcttcct tcttttacta tcaagacctt
     5701 tggatggcta tccggcaggc ctgccaaata atcgattcct tcttggatag tttcaaaatc
     5761 cgtcggggta tctatagcat tctttccaat tatcagtttg tctttcatcc tcataaatgc
     5821 tcccttatga attgtttcca aatatttctc atttcttgtg tttccatatg accgcctgtt
     5881 aaagaaaaag aagaaaagtt ttctggacta cccattgatt gatacttttt gtccagccct
     5941 tttctcagct tcagtacgcc ttcgtccaag cacatcgtat cgttttttcc aactaagcta
     6001 agacgagggc gcggagcgat tttttcttgg attgctaacg ttgtataagc tgtcaataga
     6061 tttggcacat agtaataaaa cccatgatga tctaggcggc gatgtagcaa aagtgtttcg
     6121 atgtccacct gtgccgctag atcaatacaa actttgacac gagaatccaa tgctgtcacc
     6181 caccagctta ataagcctcc catagaaaga ccaatcgttg cgagccgttg agtatctgtg
     6241 tcttcccttg tctccaaata atcgatcaag ctgatcacat cataaatacg cattccccat
     6301 aacgtttttc ctgttaataa aaattgtttg aacaactcgc tttcagaaat acctccgcgt
     6361 tcttcaaatc cccaagcatc aatcgaccaa acagcatatc ccattcctgt cagtacttct
     6421 gcaaaagatg gagaagctaa gtaaggagca ccatggatca attcactttt cccgactgaa
     6481 aaatctccgc cgtgagaatg caggtaaagg accgtcggaa acggtccttc tttttctgga
     6541 taagcataga gggctggtac tgtttccaat tcatttaagt acaattgata gtattcaagc
     6601 ataaaaccgt tttgtttttc tcggtataat cgttttccac taggggtatg tctgctagat
     6661 ggagcaccta aaaaatcatt caactgcata agcaaagttt ttacgaacaa ccagttcatc
     6721 catattttct gattgatttg attggaattt ctcaatactg atttctttag tattttctaa
     6781 tagaaatgct ggtccctcgt gattttcaat cgacatctgg ttaaaggaaa ccttacccgc
     6841 aaagccaata taaaatcctt gtctgctcat ttcctctatt cctgtcatca tagcaggaac
     6901 accgggaact gcatcttggg ccatcgagac atcgatattg tcaaatgtga tttcttgtat
     6961 gaattgttcc gctaatccat aaataaaacc tgcagaagca tggacattcc ttgccgtgat
     7021 attcgaaaaa tggatccttc tgaatgctga tgtccgttca tctatcggat aagccttttt
     7081 ttcccataca tacggttctt tccctcgcgg accacagaaa taataaagat tcagtataaa
     7141 tggacacatc acattatcca tgactatatt ggatactcga atatcttcaa cgatcccgcc
     7201 tcgacctcgt ctagatttca atcgtattcc tcgatcagtt tcttggaaga tacaatttga
     7261 aatggtaata ttacgaatac taccactcat ttcacttcct aatacgacac cgccatgtcc
     7321 atggaccatc gtgcaatttg tgatcgtgat attttcgcaa gcgatccttt cgtacgtatc
     7381 ttcagtccct gccttgattg caatacagtc atctcccacg tcaatatggc aattactaat
     7441 acgcacattt ttgcatgatt ctggatcaat tccgtctgta tttggtgaat ccgctgggtt
     7501 caagatcgtc aagttatcaa acgttgcatt actgcaaaga atcgggttga tcgtccaact
     7561 tggtgactgg atcaatttaa tatctttcac agtaattcga tgacagttat ggaaactcat
     7621 caatttagga cgaggataag cgagattatc tggttcattt ctaaaagtat gccaccattt
     7681 ttttccattt ccatctaatg taccgaaacc agtgacagag atgttctcta cattttgagc
     7741 atagatacag gaagcataaa cttttcgatg gactccttcc caacgtgaat gaacaacagg
     7801 ataatctttt ggatcgtcag agaatttcag aaccgctcct gcgcttaaat gaagttcgac
     7861 attgcttttt aaaaataaag ctcctgttag aaattcacct gctggaacaa ctacggtccc
     7921 gcctccgtca cttgctgcag catcaatggc ttgttgtatt gcttcagtat tcaattcatc
     7981 aattgatgcg ccaaatgtca aaatatcata catatttata atgcctccac ttcaatcaat
     8041 gcttgcaaga atgcaccaac gcctttttga tcattacaaa taattggttc actgatgtag
     8101 taagcatatg aaccatctcg ctgatctgct ccgcctaacc ctgctacttg acagttttta
     8161 ttcaagttca gccagccttc ttttgtttcc agaacaaatt catcaagtaa tccttgatgt
     8221 gatttcttca cttgtggaag ccattcttct ttgttcagca gtcccaaacg tattccttta
     8281 gccattgcgt aaacaaacat actactgccg gaagcttcca gataatttcc tttccgtttt
     8341 gtttctgttg ttacttgata ccatgtcccg ctgttttctt cttgatatgg aagtaatgca
     8401 gccagtgtct tttctagaat ttctttcagc aaagaacaat ctgtttcttc atgaagtaat
     8461 tcataggtat ctgctgcagc catcaaatac cagccaatcg atcggctcca aaaatgatgg
     8521 gatagacctg tttttggatc agcccatggt tggaccttct tttcatccca tgcatgaatc
     8581 agcaaatttg tttttggatc gaatagatgt ttataactga tttcaaactg tctcaaaaca
     8641 tcttggtaat ctttttcttc actaaaattc tggataaatt cagcataaaa aggagaaccc
     8701 atatataagc catccaacca aatttgataa ggataaattt ctttatgcca aaaagctcct
     8761 tcggatgtgc gtggatggtt tgccaactgc tgggaaagga gttctgctgc ttttttatat
     8821 ttttcttcgc ctgtttcttt gtacaataaa aagaaaagct ttcctgtatt tacatggtcg
     8881 atattgtatt cttcaacgct gtagccacgg atcgttccat cttcttgcac gaaatagtcg
     8941 atattgtctt ttatgaaatc aaaataaatt ttttcttggg ttcgttccca aagtaattgg
     9001 aaccctttaa gtacgacgcc atagtcataa gaccattttc ctttatacca cttttcttca
     9061 tataagcgag gtgttctttc catgatactt tttgctaatt tctttgacca gttttccatt
     9121 atttgctctc ctttaaaaat taaaatagga tttgggacaa ataccaagaa gtatttgtcc
     9181 caatcgtcta tattactgtg ccgctgcttt gtatgcttcc tcgtactctt gtgtgattcg
     9241 atcgcctcct tgtgctttcc agtcttctac agcttttttg aatccatctt cgtcaagctg
     9301 tcccatgatg tattgcaccg tagcatcaat gacgattttt tcaatttctg atcctacttc
     9361 attgttcgtc ggtgaatcca atggcacaga aggatccatt acagcaaatt cttcattttc
     9421 tttgatcaat tgattagaga attctttttc agggtctgta tctttgaaag agaacgtcac
     9481 ttcactagga cgagagcttg ataacggttg cacctcttgt tgccaaagat ccatatcgat
     9541 aaattcgata gaaccatcat cattcaattc ataatgcgtt ccttcgatac cgccagtcat
     9601 caatttatac acatcttctt ctaaaagatc atcaacaaat tgaagtaatt tcttcaattg
     9661 tgcttcgtct tttacttgag atttgggaaa tgctagcaag ccgccaatac catttccttc
     9721 tgaccaaaca tgataaccgc cgccttcacc atctgagatc ttgtttacag gcactaattc
     9781 catatcatct tgaatacctt gacccatcgt cttcagattc ttgatatcca ccattccagt
     9841 gtagattcca gttcttcctt gtgcaaattg ttcttgctga tcagtctttt gggtaactgc
     9901 aaagtcttgt gccaaatagc cattctcaaa tagatctctt gaaaaattca aagctttcat
     9961 atactcttct gtatcaaatt ctggagtgaa tttgccatta tcgtctacag cccagccatt
    10021 tggtgctcca aaataagaag tgaaaagttt aaagctggta taacgaatat cactaggcgc
    10081 acgatcgcca aaaccaactg tgtcgtccac accatttcca tcaggatcgt tttctgtaaa
    10141 ttgtttagct acttcataaa gctcatccat ggtagttgga acatctagtc ctagattatc
    10201 caaccagtct tttcgaatga ctaagccagc acgagcgtag tctttttgta tcggtacgcc
    10261 gtacaaggca ccgtcgatac tcgcagcttc gattctatct tcagaaattt gacttaaatt
    10321 tttatagtct tttaaatatt ctgatacatc ccagaatacg cctgatttta acgcattgcg
    10381 gacagaagag tttttcaaca tcgcaatttg aagtgtaacg atatctccta gttcatcaga
    10441 agccaatgca gttgttaatc tttcttcttt tgaagcatca ggaatccagt tgaatgtcag
    10501 ttccgtatcg gttttttctt cgatcaagtt cacaatcgtg tctgtaggcg gtgaagcatg
    10561 atgcaaaata tttaaccaag tgattttagc ttttccatct tctgtctctg aagaactgtt
    10621 acttgaactg ctgccgcatg cggcaagtgc tagaattcct gcaaaactaa gacctaattt
    10681 tacgagcgtt gcttttttca taaattttct ccttcactgt tttaataata tttttgtttt
    10741 cttctgtttt ttctgtatat gagttcttcc attccaaatg gcaaaatata gaatttgctg
    10801 tccacgtgac tgcggaaaag aagagactgc caccgataaa ggttaaaaca cctggcattg
    10861 tttggatgat catcagataa atccctacca tacttaatat caacactaat gagtaatgaa
    10921 gatgtgagaa tccgaataaa aatgccattt tcattttgaa aaaatatcct ttccaatcat
    10981 aatgagccat gattgggaaa atatagagtg ctgagagcac atatatgaac gcagcaatat
    11041 acaaagcgat cctaagatac caattgtgta tgaaagaaag atccacaagt aaaacaatcc
    11101 cgatggcgcc atatatccag ctcatcagca aggtttcacg atagttttct ttaaaatatg
    11161 tgaagtacgt tttcgtcaaa ggaaacgttt cttttgagcg cagccatttt ctaatcactg
    11221 caaccatcgc atacgatgca ggaccgaaag taaaaactcc caaacctgcg atgactaaac
    11281 cgccccacag aaaattcaaa taaaccaact gtaaaatacg atttagcctt ccgtttattt
    11341 tatctaatga ctgcagcata tctttccctc tctttctatc ttagtaaacg ccgtcttctc
    11401 ctagcttttt ggcaatctta ttagctaaaa ttaccataaa caatcctacg acccctttaa
    11461 acaatcccat tgctgtactg aaacttaact gtccattctt caatccagct gtataaatat
    11521 aggtgtcgaa aatttctgca acgcttcgat tcaatgaatt caataacaag tacatatgct
    11581 cgaaccctaa gtctaatgtc tgaccgattt ttagaatcag caaggtaatg attactggac
    11641 gaattgccgg caatgtaaca tgccatgttt tgcgtaagcg tcctgctcca tccatttctg
    11701 cagcttcata caactgtgta tcgactgcag ttatcgctga aaggtagatg atcgtagacc
    11761 agcccaactc tttccagatc acttgtccga tatatactgt gcgtgtccat tcaggggatg
    11821 ttagaaaact gattttttca aatcccattg ttgcaaggaa ttcattcaat acccctccgt
    11881 caacattcaa aaatacataa gtaatcgaaa caatgattac ccaagacatg aagtgaggga
    11941 tgtaaatgat cgtttgaatc ccgtttttca atcgtttgtt cgttacttca tttaatagta
    12001 aagacaaaat gattggcatc ggaaaaaaca cgaagatatt taatccgaac aagatcaacg
    12061 tatttcttaa taatgtaaag aatgtcggtt ctgtaaacaa acgaatgaaa tgtttgaaac
    12121 cgacccaagg acttcccatt attcctaaaa atggttgata atcttgaaaa gcaataacga
    12181 gtccccccat aggaagatac ctaaaaatca agaaataaag gactcccggc agaatcatca
    12241 catataaaaa tttattcgtt ttgattcctg caattatttt ttttcgtctt ctgagctttt
    12301 cttctttttt cgatagcgtc agttgaaatg gttcttgttt ctttacactt tccataagcc
    12361 cctccttagt tttgtgaatg tttgctgctt atgacttcat gatagataac tatctgaatt
    12421 acgtatataa tcatttttat cgtaagtaaa aacgcttaca tagcaatgat ttttgtgcta
    12481 tacaaaacga taaagtgtat tttttcacaa ataaactacg catagcaaac cttctatttt
    12541 tttctatctg acacttagca taggaaatga ttatatacgt aatctttcac cgatgataaa
    12601 ttgaacatat agaaacagaa acatttaatt tattttgagg aggagcctat ctatatgaaa
    12661 aagaaagtct ctataggaga aagaagtttt acacttttca acaatacgtt tctaattttg
    12721 ctagctttga tctgtatcgt tcctttttta aatatcatcg ctacatcttt tgcttctaca
    12781 caagaagtcg tcgcgaaaaa gtttatcctt tttcctacta ccttttcttt agacgcgtat
    12841 cgctacattc tttctacgcc gaccattttt agagcacttg ctgtttcgat tggtgttaca
    12901 ggtgtcggaa cgatcgtcag tatgtgtgca acttcactga tggcctacgg tctttccaga
    12961 aagtatttgt ttggtcgagg tttcgtcaac tttcttgttg tattttctat gttgttcagc
    13021 ggggggatga ttccaacgtt cttagttgtt cgttctctag ggctggtcaa ttcttactgg
    13081 gccatgatcc tgcctgtggc tgttaatgca atgaatatga tcattatgag aaactttttc
    13141 caagccttgc cggatagttt ggaagaatca gcaaaaatgg atggttgcac agattttggt
    13201 gtatttttca aaattatgct gccattagct ttaccgtcta ttgctactat ctcgcttttt
    13261 tatgctgtta cttactggaa cacttatatg actgctattt tgtatatcaa cgattcatct
    13321 aaatggccga tacagattct tttacgccaa atcgtcattg tatcaagtgg gatgcaagca
    13381 gaaagtagtg ccgttgatgt gattccacca gcacaaacag ttaaaatggc tgttatcgtt
    13441 attgcaacag tgcctatgct gattgtttat ccttttgttc agaaatattt tgtcaaagga
    13501 gcactcgtag gttctgtcaa agggtgatta tgtactgaac gcaaaatggt agttgtgtca
    13561 gaagtggtca gcttcaagaa ataagaaggc atttacgaaa attgcttctt aaatttttgt
    13621 gaaatttcgg cttatttttc gaagaatcta ctcctggaac accgtgtatc agaggttgtg
    13681 aaagagacgt tcagccttga gcgatgataa aaactctcac aaacattgcg acgagtaacc
    13741 cacaggagca ataagaagcg attttgaaaa atagcttccc atatttcaca aaattatgcc
    13801 ttattgtcga tagcctgtct cttgaacgcc atttattcgt aaaaaagaac tgtgacaaga
    13861 cttttgtcac agttcttttt gcgtgcttag ccttgaaatt taactatcca taaatacaaa
    13921 tactagggtt attctaactt cttttattgc ttgaatcagt ccacaagaaa aacagcctct
    13981 tgattaaacg gcgtttatct tgttgattct gcggcaataa aatcagccag cagcaaaaca
    14041 tgaagtgctg ttttttgctg tcctctttta ttgcttgaat cagatcacaa gaaaaacagc
    14101 ctcttgattc acaaatattt ttttcgatat tctcccggcg taatcgattc cttcttttta
    14161 aagaaacgaa taaaattttg cggatttcga tattgcaatc tttctgcaat ctcttttacg
    14221 gagaggttag tctctttcaa ccattttttt gctacctcta aacgatgatt ttgaacaaaa
    14281 tcaccaaatt tttctcctgt ttccttttta aatacattac ttaaatagtt agggttataa
    14341 tgcaacctat cagcgatcac atctaaagac agatcacgat catattctgt gtagacgata
    14401 tgcataatat tctccgacaa atttttaaat tcatgatcag tgattgcttg agagcattct
    14461 gtgattggca gaactaattg ttctaaaaca agtttttcta gttctttggg atgataattg
    14521 ttcagtgctt ttacataaat ttgtttcatg ttatcaaagt tttttgaatc tactcccaat
    14581 aactgtccta attgaacgag ttcattgatc aagcggaccg aaactacttc ccgactcaaa
    14641 ggatttttat tttgtgcaaa caatgcatca atcaattcat gtactaaatt cttcacttcc
    14701 tgatttccag aacgaatggc ttcaaacaat cgattttgtt cttctatcgg atatctaata
    14761 agtgtttttt catgagcagc aggaacgatt tcctgataaa aaataattga acttggacca
    14821 gtatttacac gataatacag ggcttctttt gcccgatcaa ccgattgctt gctttcattc
    14881 agcgtttcga accggttact gatgcctata ctgatagtta aattcaagta ttcttttaca
    14941 cgtttttgaa tgagtcggca atactccatg acttttttgc ttgcttctgc atcatcttta
    15001 tccattcgat aaatagtggc ttgcatttct tcattcaaaa cgatcggcag cattcgattt
    15061 tcctttggca caatttccgt gatcatgttg ttgatcgcta aaagtagcaa atcacgctcc
    15121 ccaccgtgac tatcttctaa cagatcgatt tgaaccaaag ctgtataata gcagtcattt
    15181 tgagaactat aaccaaattg ctgcaaccgc tgatctagtt ctcgcctttc tactcgattt
    15241 ctaaataaac taagtacaaa tagcgtttcc agctgtggct tttgggtaac taagtgtgca
    15301 cttaacgctt ctttttctcc tatgatccga ctgactgatt gagcgaccaa atctagttct
    15361 ttcccactga atccttttga tttaagattc agacgttcct gtatttgaga aatcggacgg
    15421 gcaaatctgt ccgaaaataa gtaggaaaaa agaccaatga gtcctaccag tgttaaagac
    15481 actgcaacta gcccgattct cagattgcgg atcattgcgc caattgcact agagtcaact
    15541 tctaatctgt aaatccaatt attataatct gatttcagta ctatatacgt tttatctttt
    15601 tctcttatca ctttcagctg attgtaatct agatccacgt ccgttaatac agttggttgc
    15661 tctttatttt ttactgtctg agtagtaaaa aggttgccaa cttcgttttc aatagaaagt
    15721 atgtcttctc cactttcacc aacaacttgt tctattgtat gtttctctat cgtagcaatt
    15781 cccaatgcat atttttcttt ttcaaacatt ggcaaagaaa tcaccatttc gattcctttg
    15841 tttttctttt gccaaaataa attatttttg ttatttatgt acttctgttg atacttttca
    15901 acttcctctt cggtcagttg actcaacgat ccattgatta ctccccattt accagccaaa
    15961 ctgactaaat cataattatt atcatccata acaatcgttt ctataaaatt tagttctttc
    16021 ctgatttcag aataagtgga gtaatctgta tatgtcagcg gatgcttgaa tatgtccgag
    16081 aaatttttgg tcgaaccata cgtagaaaac gaatattcaa tcgttctaat tttccattca
    16141 acgttagttt taacttgcct gagcaaattc tttttgtctg ttaaataact cgattcgatc
    16201 gattcatagg tattctggta aatgaataaa ctaaatccta tgacaagtac ggctcctaat
    16261 aaaaacatcg gtaaaaatat tttataaaat actgtacttt tcttcatttt cttcctctcc
    16321 tccttttaaa ataaaaggct ttcatttttc tgtaaaattg atactcatct aattaaatga
    16381 gcattccccg ctttcaagaa tagcataatt aaaaataaaa atccaagtaa tttttaacgt
    16441 gtgcaatctt ttcatatcaa agatctattg acttatcgta gttatcgtaa tatgatgaat
    16501 gcgttaacaa aaagtgaaaa agtaaggaaa atgatggaat ggtaacgatc aaagatatcg
    16561 ctaaagcagc tggcgtttct catacgacag tttctcgtgc tttgaataac agcagtttaa
    16621 taaaaaatga aacaaaacaa aaaatacgac aactagctaa ggaaatgaat tattcgccaa
    16681 actatagtgc aaaaggttta gtgaatcaaa aaaaatatct aatcggacta tttttctcaa
    16741 gtttgcataa aggaacttca tcaaatttcc tagcagattc cattgcaggc atccattcag
    16801 tattagacac aaattacagt ttatctgttg aaagtatcga tgttgttgat ttaacagaaa
    16861 tcaacctgca acgctacgat ggactgatcg ttatgagtca aagtgatgat gatcagcctt
    16921 tcatcgacta tctcaaagaa aaagaatttc ctttcgttgt tgtcaatcgt cacatcaatg
    16981 atccagcggt tatcaacgtc actgccgatg atgcggccgg tgttatgcag gcagtagatt
    17041 acggtattgg atgcggacat acgaagatcg cttatattgg cggaaagaaa aattttcgtt
    17101 cgacaaaaga acgggaaaaa ggggtattga aaagtctaaa aaaagcgagc atccctgtca
    17161 atctagccta ttttttaaca ggagattaca gcttagaaag cggcctccaa aacatggaat
    17221 acctattatc actttctgac ctgcctactc tggttttttg cggcaacgac gacatagcga
    17281 ttggtgcatt gagagctgta catttagctg gcctccgtgt tccccagaac atttctctga
    17341 tcgggttcga tgattctcca gtcgtctcct atctcaatcc gccgcttacc accgtacgta
    17401 agcctttgaa agaaatgtgt caagaaggaa caaagctttt actagatttg atcgaaggca
    17461 ggacgatcga acaatctaaa attgagctct ccaccttgtt gatccttcgt gagtcgatca
    17521 attacttgta aactacaagt aattttatat aaaaatgtta acgtgtgcat aacacagaaa
    17581 ggaagtttat atgaaaaaac tgacaaaaga attagtgaaa aaaaacgaag cacctgtaaa
    17641 agttcttcag tttggtgaag gcaattttat gcgtggattt attgattggc agattcagca
    17701 gctgaataac caacaattgt ttaaaggtaa tgttgcaatc attcaacctt tagaacaagg
    17761 tcttggaaat atgatgaacc agcaagataa tttatatacg gtcattttac aaggactcag
    17821 caatggcgaa atcgttgata cttccgagat tatcaccgta gtagaacaaa tgatcaatcc
    17881 ttatgatgaa tgggatgctt atctatctct agcagaaaat gacgacttgg cctatatcgt
    17941 ctccaataca acagaggcag gaatccaatt cgtggagact gataatttaa cagatactcc
    18001 cccacagagc ttcccgggca aactgacagc ctttttatac cgacgtttca aattaaacaa
    18061 agctggcttt accatcattc cttgcgaact gattgacaga aacggcgaac gtctaaaaga
    18121 aatcatttta cgctacgcag aaaaatggga tttagaacct gcttttgttg agtggttaga
    18181 aaaagaaaat gttttctgtt gtagtttagt tgatcgcatc gtcccaggtt atcctcgcga
    18241 agatgcaggt gttttcaatc aacggcataa ctatgaagat caattgatgg tcaaagcaga
    18301 accattcatg ctttgggtga tcgaagggcc taaagaattg gagaatcaac taccattcaa
    18361 acaggctggc ttgaatgtca tcatcacaga tgatatgact ccttatcgtg aacgtaaggt
    18421 gcatttgctt aatggaccgc atacagcgat ggtccctctt gctttgttgg cggaattgga
    18481 aactgtagaa gaagtaatga aagatccttt attctccgca tatgttgatc agttattcaa
    18541 tctagaattg attcctatgc tttctttacc taaagatgaa ttagccattt atgctgacca
    18601 aatcaaagaa cgtttcttga atccttttgc ccatcacaaa ttagaagcga tttcattaaa
    18661 tagtgtttca aagttcagca cccgtctgct tccagtgttc aagaagtata tcgaagaaca
    18721 aaatcaggta ccaccgttaa tcactgtttc actagctgcg ctacttctta tgtatcgagg
    18781 cgatcaagta aagccacatg atgatgaaaa gacgataagc gaatttactg atgcttggtc
    18841 tgataatgga acagctattc ctcgattatt gcaaaacgct gcgctttggg gagaagattt
    18901 gtctcaaata ccgaatgtga cagatacagt tcaagaaata gctgcaaaga tcgatcaaaa
    18961 aggtgtccgg tcagtattac aagaaatgaa aggaacgatg gtccatgctt gaaaccctgc
    19021 aaccaacaat gaaattaaat cgacgagaca gtgtggtcgt cgcattaacc cctattccaa
    19081 aacaaacaca actgacgatt gatcagcaaa cgatcaccac tcttgaagat atcccgcaag
    19141 gtcataagat tgcattgggt gatttgaaag ctggagacaa cgtgatcaaa tatggctatc
    19201 caatcggtca tgtgacgaca gacgtaactg ccggtcaatg gctgcacact cataatgtaa
    19261 aaaccaatct ttctggtgaa ttggattatc gatatgaaaa agatgtccat ccaagtcatt
    19321 acctttttga gaaccgaaca tttcaaggtt acttgcgaaa aaatggaaaa gtagggattc
    19381 gtaatgattt gtttttagta cctaccgttg ggtgtgtgaa tggcattgct gaactgatcg
    19441 tcaaacagtt caaggaaaaa catccagatc tgggacagtt tgaccatatt acgattttaa
    19501 agcatcctta tggctgttcg cagcttggaa aagatcatca aaatactcga gaaattttag
    19561 cggacgcagt aaatcatccc aatgccggag gagttttagt atttggatta ggttgtgaga
    19621 acaatacagt tcctgaattc aaaaaaattt taggtgaata tgatgaagaa cgagtgaaat
    19681 ttctagtggc tcaagatgtt tacgatgaga tcgaacaagg tgtggctcta ttagaagagc
    19741 ttttaacagc ggcggaaaat gaccagcgca tatcagtccc gctctctaag ttgaatgtcg
    19801 gtttgaaatg cggcggttct gatggtctat cgggaataac tgctaatcct ttacttggtg
    19861 cattttctga ctacttgatt gctcaaggag gcagcacgat cttaacagaa gtgccagaaa
    19921 tgtttggcgc agaacaagta ttgatggcac gagcggaaaa tgaagaagtt tttgactcaa
    19981 tcgttgactt gatcaatgac ttcaaacagt atttcttgtc ttacggggaa ccagtttacg
    20041 aaaacccatc tcctggtaat aaagcaggtg ggatcactac attagaagat aagtctttag
    20101 gctgcacgca aaaatcagga agctctcctg tcgttgatgt gctacaatat ggcgaaaaaa
    20161 tcagaaaacc agggcttagt ttattgcaag ctcccgggaa tgatcttgta gctgcctctg
    20221 ctcttgcttc atccgattgc cagttggtct tgtttaccac aggaagaggg actccgtttg
    20281 gctcatatgt cccaacggtc aaggtatcta cgaatacaac tctttttgaa cgcaaaggtc
    20341 attggatgga ttttaatgca ggcgtcttat tagaacaacc aatggaagta atcttagaat
    20401 cgtttgttca aaaaatcatc gctgtagcta gtggagaaga aacgaaaaat gaacaaaatg
    20461 acgttcgaga aattgccatt tttaaaaacg gagtaacact ttgattttca taaaacagta
    20521 taaaaagaaa gaaggaagat ttatgttttt atctgatgat tttttattaa aagacgattg
    20581 ggcgaaaaag ctttaccatt cctacgcaaa aaacatgccc attattgatt atcactgtca
    20641 cttagatcca aaagaaattt acgaaaacaa caatttcagc aatctgacag aagcttggtt
    20701 aggaggcgat cattacaaat ggcgcttgat gcgagcttgc ggcgtgcctg aagaaaaaat
    20761 cacaggaaat gcttctgatt ttgaaaaatt tttagcttgg tgtcaaaccg taccaaaaat
    20821 tatcggaaat ccgctttaca gttggtcaca tcttgaattg aagcgtttct tcggtattga
    20881 cttgttgata aatgaagaaa atgcagagaa aatttggaca caagcaaatg aaattttggc
    20941 agctcctgat tttcgccgtc gggaaatcgc aaaaaaatcg aatgtagaag tgatttgtac
    21001 aacagatgat cccgcagacg acttgcacta tcatcagttg cttgctcatg aagaaccaga
    21061 tttgaaggtt ctaccttcat tccgtccaga taaagcactc aatattgaaa aagaaggatt
    21121 taatgcatgg gtcacactac tggaagaagc agctgaccaa acgatcacca cctatcaaga
    21181 actgatagaa gtcttagggc agcggataga atacttccat caacatggat gtcgtgtttc
    21241 cgaccacgct ttagacatct tacgctataa agaagcggat gaaacagtat tagaagagat
    21301 ttttcaaaaa gcacgagcta acaaactatt atcttcagat gaaatcgatg cttatcttac
    21361 agaaacactg atacatttga cccacttcta tcattctcat aattggacga tgcagctgca
    21421 catccatgct tatcgtaact gtaatacaca aatgttggaa aaattagggc ccgataccgg
    21481 ctatgatgga atgaatgatc tttctttgac tattcctctg caaaaattat tgaaccgtgc
    21541 agaagaaacg gatcagttgc caaagacgat cctttattca ttgaatccga atgactatcc
    21601 tgcgatttta gctttgatgg gctgtttcca aaaagaacag aatggaaaac tgcagttagg
    21661 ttctggatgg tggtacaacg atactcgagc tggtatgcgc gatcaattga cacaatttgc
    21721 tgatggtagt gctttgggga attttgtcgg catgctgaca gattcaagaa gtttcctgtc
    21781 ctacactaga catgaatatt tccgtcgtgt tctttgtgaa tttttaggcg aaatggtcat
    21841 gcgaggagaa gcacctgaag atgaacaact tttaggaagt ctagttcaag cgattagtta
    21901 tacaaatgct aaaaattact ttggcttttt tgcctaattg attaatagta acagtaacga
    21961 atcaaaaaca ggatagtgaa ataacggaat ccgtttatcc acgttttaga gaagaagacg
    22021 gaccaa
//
LOCUS       DACDUU010000050        21664 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.050, whole genome shotgun sequence.
ACCESSION   DACDUU010000050 DACDUU010000000
VERSION     DACDUU010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.050"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            23..262
                     /locus_tag="IWL30_001855"
     CDS             23..262
                     /locus_tag="IWL30_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706609.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(624..884)
                     /locus_tag="IWL30_001856"
     CDS             complement(624..884)
                     /locus_tag="IWL30_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706610.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1069..1566)
                     /locus_tag="IWL30_001857"
     CDS             complement(1069..1566)
                     /locus_tag="IWL30_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAP9706611.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1696..2406)
                     /gene="budA"
                     /locus_tag="IWL30_001858"
     CDS             complement(1696..2406)
                     /gene="budA"
                     /locus_tag="IWL30_001858"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAP9706612.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(2419..4083)
                     /gene="alsS"
                     /locus_tag="IWL30_001859"
     CDS             complement(2419..4083)
                     /gene="alsS"
                     /locus_tag="IWL30_001859"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAP9706613.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(4288..4950)
                     /locus_tag="IWL30_001860"
     CDS             complement(4288..4950)
                     /locus_tag="IWL30_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9706614.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(4960..5775)
                     /locus_tag="IWL30_001861"
     CDS             complement(4960..5775)
                     /locus_tag="IWL30_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9706615.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(6037..6480)
                     /locus_tag="IWL30_001862"
     CDS             complement(6037..6480)
                     /locus_tag="IWL30_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAP9706616.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(6614..6952)
                     /locus_tag="IWL30_001863"
     CDS             complement(6614..6952)
                     /locus_tag="IWL30_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9706617.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(6940..7317)
                     /locus_tag="IWL30_001864"
     CDS             complement(6940..7317)
                     /locus_tag="IWL30_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9706618.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            7541..8734
                     /locus_tag="IWL30_001865"
     CDS             7541..8734
                     /locus_tag="IWL30_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9706619.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(8900..9322)
                     /locus_tag="IWL30_001866"
     CDS             complement(8900..9322)
                     /locus_tag="IWL30_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9706620.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            9717..9911
                     /locus_tag="IWL30_001867"
     CDS             9717..9911
                     /locus_tag="IWL30_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706621.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            9908..10402
                     /locus_tag="IWL30_001868"
     CDS             9908..10402
                     /locus_tag="IWL30_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706622.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(10564..11736)
                     /locus_tag="IWL30_001869"
     CDS             complement(10564..11736)
                     /locus_tag="IWL30_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9706623.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(11942..12757)
                     /locus_tag="IWL30_001870"
     CDS             complement(11942..12757)
                     /locus_tag="IWL30_001870"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9706624.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(12907..13353)
                     /locus_tag="IWL30_001871"
     CDS             complement(12907..13353)
                     /locus_tag="IWL30_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP9706625.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(13350..14354)
                     /locus_tag="IWL30_001872"
     CDS             complement(13350..14354)
                     /locus_tag="IWL30_001872"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP9706626.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(14394..14723)
                     /locus_tag="IWL30_001873"
     CDS             complement(14394..14723)
                     /locus_tag="IWL30_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP9706627.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(14849..17176)
                     /locus_tag="IWL30_001874"
     CDS             complement(14849..17176)
                     /locus_tag="IWL30_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9706628.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            complement(18019..18312)
                     /locus_tag="IWL30_001875"
     CDS             complement(18019..18312)
                     /locus_tag="IWL30_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAP9706629.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(18715..19893)
                     /locus_tag="IWL30_001876"
     CDS             complement(18715..19893)
                     /locus_tag="IWL30_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9706630.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(19997..20311)
                     /locus_tag="IWL30_001877"
     CDS             complement(19997..20311)
                     /locus_tag="IWL30_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9706631.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            20418..20633
                     /locus_tag="IWL30_001878"
     CDS             20418..20633
                     /locus_tag="IWL30_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706632.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            complement(20933..21175)
                     /locus_tag="IWL30_001879"
     CDS             complement(20933..21175)
                     /locus_tag="IWL30_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9706633.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(21162..21590)
                     /locus_tag="IWL30_001880"
     CDS             complement(21162..21590)
                     /locus_tag="IWL30_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9706634.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
BASE COUNT         6362 a         4268 c         3671 g         7363 t
ORIGIN      
        1 tactataagg agtgtgtaca taatgaaaag aattctgttt atcagtttat caaatttatt
       61 gttacttact gcttgtggga cttctgcatc aaagaacata ccttcagata cgcaaatttc
      121 gaatagtatc gattcaacaa tttctacaaa tgagaaaaat tctccggtag aaacttccga
      181 tataaaagtt tctacctctg caaaaaacag ggtaaagaaa ctgtcagcac gtctccttta
      241 tttcaaacaa actctgaatt agcttcttca tcttcttcag caaataatga tgatagtttc
      301 gcgtttactc ctaactaaat gcaaccttta caaatgcttt acgataacaa gcctgagcta
      361 aatgatcctg atatcagttt tgcatttttt gaaatgattg gatcggatga tctttttaaa
      421 actactagtt tgtctatccg aaaataggac agttctggca gtataggttt ttatcgagtt
      481 tctccaccag gcctcgttac agagacagat tctctaggcg aaccttttga ataaatcaaa
      541 aatcatgaac aatcactgcc aactaaataa agtgccttca aaagcttggt cttctcgctt
      601 ttgaaggcac tttatttaaa tctttatgaa actaaagtat gttcatattt gttgctatag
      661 ttatatagat attctgttgt ttccaactca taaacgtgag tatattttgc cactactttt
      721 ccgcattttt ctaatatttc attgcgatcg acgtcatgat attcgtctac gcagatcaca
      781 acacaatttt ctaatttact gattacttct ccaaccactt tttgtttcaa gccaataggt
      841 tggcaaatat atttttttcc taaaatgata gtttcgttat tcatatttca tccatcctta
      901 atgtttttcc attatttaaa atgataatac cattaaaata tgattatgaa aatcaataac
      961 acttgtgatt ttataaaaaa ataaaaaaac cgaaaaacag aaatgcccac tgtctttccg
     1021 gctcttttta ttcttgattt ctcttctttt ttgtttcaat agacgatttt agttattaat
     1081 aaatgaaaaa gcatttatat taaaatttgc taataaaaga tctacatatt tttctagaaa
     1141 tgcttgatcg atttcatcat accctttcgt tactccgcaa tcgatatcta aaacaccgat
     1201 taaattacct tcttttacca ttggcactac gatttctgat tgtgcggctg aatcgcagga
     1261 aatgtaattt gcatgggttt tgacatcatc aacgatcaat gtttcttgtt tttcagctga
     1321 ttctccacaa actccttttc ccatcttgat acgtgtgcaa gaaactcgcc cttggaacgg
     1381 tcctaaaatc aattcattac catcgaataa ataatatcct gcaaagacag tctcaggcaa
     1441 agtctcattc aacaatgcag atgaatttgc taaatttgct aaccagttat cttctgcttc
     1501 taataatcct tgttgctgta ataataaaat ttcgtatgca tcttttttct gtttttctgt
     1561 ccacatgata aaaaccgctc ctttttgatc tatggagaag attatagctc agtttttttg
     1621 taaacgctag gtttcctcct caaaaaaacg agatacgtca ggttaatcac cttgaacgca
     1681 tctcgctgtc tttttctatt ctgcttgttg gatttctgct gctaaatcag cataatcaat
     1741 cgtttcgtga acaaaagtcg gatcatcaac tggaaaatgc tgtacaaggg acgagatgcg
     1801 gctaatctca acttttcctt cagccacttc aaaatccatt acatgtccgc caaatgtatg
     1861 tgtatcatcg ataaaatgca agtgaaatcc cgctgcagca actccttgga ataattcagg
     1921 cgtaaagaac ccgactaatg ttccttggac attgctttgt tggaattctg gttgagactc
     1981 tgaaattttt gctagtcttc tgtaaggttt ttcttgcttc ggcatgatac ggatgtgcat
     2041 atttttgaat aatccagtta tcttcactgc caaaaatagg ttttccccgg cttctcttac
     2101 taatatgtct tttttcaatt gttctccatc tgcagttttt gagacagaaa aactagtatc
     2161 tggcgtaaaa tctgtaattg ctgcataggg ggtaagttca cttccatcta attgggcaac
     2221 tgagccatct gatcttcctt gataagctac gccatctaaa aagatgactt caccatcaag
     2281 cccatgcaat gttcccaatc ccagagttcc tttttctaat atatgagcaa tcgtttctgt
     2341 tccatccatc aagccagcca tcaaagctcc taatgtcccg tgctggtata agattttttc
     2401 agtcatgtcg acacttcctt aatagaattg atctggtaag attgtttctc ccaatttctc
     2461 attatcacgg taatcaatcg gaatatcaat aattacgggt ccttctgtcg caaatccttc
     2521 ttctaaagct ttttctaatt cctctacact tgtagctcgg attcctttag cgccaaaagc
     2581 ttctgcatat ttcacaaaat caactggacc aaagtctaca cctgacgccc gctgatattt
     2641 cattttttct tggaactcta ccatattgta gtgtccatca ttccaaatca aatgaacgat
     2701 attcaatttt ttacgtaccg ccgtttctaa atcctgggca gagaataaaa atcccccatc
     2761 gccggaaact gatacgattt gtgtatttgg acgcaccaat gcagcagaaa tcgcccatgg
     2821 caatgcaaca ccaagcgttt gcataccatt actgaataac aaatgacgag gttcataact
     2881 tctaaagtgg cgtgccatcc aaatataatg actacctaca tctacggtaa cagtcatatc
     2941 atctgttact tttgactgaa gcgtgttaat aacttctaaa ggatgcaaga tacctgcttc
     3001 tccttttgat tgaacaatgt cccgctccgt taattctgct tgaagagatg ctaaatattc
     3061 ttttgcatct tctgacactt gttgaggctc taatgaacct gtcaataaat caagagtggc
     3121 tgagatatca ccgatcaatt ctctttcagg ctgcataaaa ggatcgattt cagctggcgc
     3181 ttcatcgatc acaatgatcc gcgcatcttt ttccgcattc cagtttcttg cttcatattc
     3241 gattggatca tagccaatag caataactag atcacttcgt ttcaaaagca tatctcctgg
     3301 ttggtttcta aataaaccca cacgaccaaa gaaatgcgct tctaattcac gtgagatcac
     3361 tccagctgct tggaatgtct ctacaacagg caatgctgtc tttccaacta attgcctgat
     3421 ggctaacgtt tctttttcac ttgatcctcg catacctact aatagtaccg gtagttttgc
     3481 ttctctaatg cgttcagcta ggtaacggat atcatctgca gaagcagaac ctaatttagg
     3541 atcgctcaat ggcttgatga cattcccttg gacaggtgca tccactacat cttgcgggat
     3601 actgataaag ctcgcgcctt tttttgaact cttagccata cgataagcat tggcaatcac
     3661 ttctgaaatt gtttctggat cttggatttc tgcactgtat tttgtgattg gctggaacag
     3721 tgctgcatta tccatgcttt gatgggtcaa cttcaataga tcgcttcgtt ttacttgtcc
     3781 ggcaattgct aaaactgggt caccttctgc tgtcgctgtt accaaacctg tagctaagtt
     3841 actagcacca ggaccacttg ttgcgataac tactcctggc tctcctgtaa tacgtccgat
     3901 tgcttgtgcc ataaatgcag cattttgttc gtgacgagtc acgattaatt caggaccttg
     3961 atcctctaat tcattaaata caccatcgat ttttgcacct ggtataccga atacataatc
     4021 aacatgatga ttaatcaaac tttctactac agatttcgaa ccttgtttca ttttttcgtc
     4081 cattgaacat cgtccttttt atgctttatt tttaattatt agttagaaat aatcacaaat
     4141 aattgataca gtttctaaac ctgtttcatt caaaagtata gcacgtttta tttcatttgt
     4201 ttagccatat gtttcaaaaa atgaaaaaga atgacagttt ttacataaaa actgtcattc
     4261 ttaattgaaa gtttcaattt ataaagtcta cagatccata gttctcatca atttacgatt
     4321 gttgaagtaa atcaaaccaa atacgataga aggacctagt agaatacacc attggggctt
     4381 tacgaaaaag aagagaaaca aacttgtcaa tagtaaaaaa tctagtaaac tcacatacca
     4441 ttttttcaac gtatagtaga acgctatccg tagaacttga tttggtttcg acgctggatt
     4501 tctcacctga aaataccagt agttgatgct aaaggaaact gataaacaag caagtaaaac
     4561 aaacaacgga atcaaccatt ttccataaga aaaacgtata aaaaagaaac aatcaaaaat
     4621 cattatcaca ctaactatcc aagcaaatag ccaataaaaa aatcccttta gccaaaattg
     4681 gcgaaacgtc tttatgaaca aacgtactgg tgaaaaactt ttttcttttg ctaattcttc
     4741 tgtcatacct atcatcacga tgcaacctgg accaataaaa agtaaagaaa aaataaagga
     4801 aaagacattc cttccatcta tcgacaaaag gaacgcagct gctatataca agatattgac
     4861 acaccaaaaa caaaggctac ttgccaatga ttgatacaac caataaaaga attccatata
     4921 aacatttttg tcaaaacgct gctctttcat aatcacctcc tattccttca ttcctgatga
     4981 agcgatccct tctacaaaat atttttgcat cgctaaaaat ataaaagcaa caggaagaac
     5041 agaaacaact gaggcagcca tgattaatgc gtactctgca tcaaatagcc caataaacat
     5101 tttcaaccct aactggattg ttttattttc cgtagaagac aaataaatga atggccccat
     5161 ataatcgttc catgtattaa cgaatgtgat gatcgtcaaa ctggcaatcg ccggttttgt
     5221 aagaggtaaa atgatcttgc gatagattcc ccactctgat aagccatcga tccgcgcact
     5281 ctcacataaa gaatctggga tactactgta gtattgcttc aacaagaaca caccaaaagc
     5341 attgaacgct tgtaatagca ccaacgacca caacgtatat gttaaaccca cttttctcat
     5401 cataatgaac tgcgggatca tataagactg ccaagggaca gcaatcgtgc cgatatatgc
     5461 taaaaataaa atatcgcgcc ctttaaaatt tagtttagaa aacccgtaag cagcaaaact
     5521 agaagtaaac aactgaatca acgtgatcac aacgcttaaa aaagctgtat tcttaaaaaa
     5581 tgtcactaaa ggaatacgag accaaataac cagataattc tctaaatgcc aagttttagg
     5641 gatccattga atcggaatcg tgaacacttc attatttgct ttgaatgaag cagaaaccat
     5701 ccataaaaaa ggaatgatcg tcaataaagc taatacaatc agcagaatca tacccaacca
     5761 ttgaataaag ggcatgtttt ttgaacgaat ttttgctttt tttctttcat ttcgaacacc
     5821 tccaaagtta taatcttcat ttctttgtaa tgattacaat ccagaacaac ggttattata
     5881 gctgattcat ttccgttctt cttatgtttg taagcgctta tttaacaagt tatgtaaatc
     5941 tctttatttt ttttgattta aaaatgaaaa aatacaaaag caagactgtc atacgtctcg
     6001 cttttgtatt gaaatttctt cgattagaga tacttattac tgttcaaaag cttctttgaa
     6061 acggtgaatc aattcattga ctacaggtga taaaatccga ttctttttcc agactagcat
     6121 gcagttcgtt tcaatcattg ggtcaagcgg aacgaatgtc actcgctttg aaagatgact
     6181 tacagcacct tctatgacta aagcagcacc tacctgattc tcaattagcg caaatacatt
     6241 gaaaatcaaa ttgtacgttc ctgtgatcag caactgctcc ttttttatgc ctaaccagtc
     6301 agcaaacatt cgctgtattt ctggacgctt tgagatcaaa agaggtacat tcgcaaagtc
     6361 ttcttttgtt atcacgtctt tttttgctaa aacagaattt tctaaaatga gcaatcccca
     6421 tttttccttt cttggtaaca aaatcgtttc gtatttctct gtatctactg gatctagcaa
     6481 aatcgctaaa tctaacaggc ttttttctag tcactccgtg atatcatcac ttgttccgct
     6541 gtggatatga aattgaacgc tgggataatc acggatcaac ttttctagta atagtgccaa
     6601 tgtattcgaa ttatcagctt ccacacagcc gatcgtaaat cggccgctga ataattgttt
     6661 ttttctgttc tcgaattctt gagtggtttg attcgttaaa tctaaaattt cttctgctcg
     6721 gcttttttag aaaataacct gcttctgtca agatcatttt gcggttttcg cgtacaaaca
     6781 gttctgttcc tagttcctct tctaactcct tcagttgtcg gcttaaggtt ggttgtgtaa
     6841 gatgtagaac ttctgcggct tgagagattg ttccttcttc tgcaattgtc caaaaatatt
     6901 ttagtaagcg taactccata gcgatgctcc ttgatttttt tacttgatac attccatttt
     6961 atttctagat aacacacgat catatttctt gatttttcct tgtaaacgtt ccaaacactc
     7021 ttgcatttca gcaatttctt tcgtcaaaag ttcactttct tcaatcaaaa gcttacgccg
     7081 ctcaaataca gttgcttctc cttcgctaga taggcgaata tattcaatca aagactcgat
     7141 cgataatccg gcatttcgca tacagatcgt aaagtcgacc cattttaggt cttcttctgt
     7201 ataatcacgg attccgcctt ttgtccgatt aactggtgga atcaacccga ttcgttcata
     7261 atagcgcaat gtatcggccg aaatgccctt ttcttcactg acttgtttga tattcatctg
     7321 atcactcttt ctcttaattt cttgccactt tacttatgta ttttatttat tctacaactt
     7381 taagtgtact ccaagtcaat actcatttct attaaaacta agctggatta tctatatcgg
     7441 ttgtttgtgt attatttcaa ataaaaaagt cttgtttttt tattcttttt tggatatact
     7501 tttaacaatt tagggaaatt ttaggaaaag gggtataaaa atgacaacgt atcagcgaaa
     7561 ggtattatta tcgacatcgg cgggaattgc tttagagaat atggatatta tgtttttagc
     7621 tttttcatta tcttcaatga ttacttcttt tcatattagt ggtacgcaag cgggtttcat
     7681 ttcaacgatc actaatttgg ggatgctcat tggaggtatt ttttttggca tcatagctga
     7741 taaatatgga cgtgtgaaag tattttctca aacggtcctt ctgttttcga ttgcttcttt
     7801 attgatgtat tttgcttcaa acatctactt ggtttatctc tttcgcttta tcgcaggtgt
     7861 aggagcggga ggagaatacg gggcgtgtat gtccttgatt tccgaaagtt tttcaaaaaa
     7921 acaaattgga cgagcttctt cggttgctgg tattggtgcg caggtcggtg cggctttggc
     7981 tgcgatttta gctgctgtga tcattccaac gtttggttgg aagatgttgt atgtcgtagg
     8041 tgtactgccg gtgttgatgg tcttgttcat tcgtcgcggc ttgactgaac caaaggaatt
     8101 caaatctgta caatctgccg aaaagaaaac caaacttagc agactgtttg aatcaaaaca
     8161 tcttgcttgg caaacatgtg gccttagtat catggtaact gtccaaattg ccggctattt
     8221 tgggttaatg aattggctgc cttcaattat gcaaaatcga ttagggcttt cggtttctgg
     8281 gtcttcattg tggatggtca gtactatttt aggcatgtct ttggggatga tgacttttgg
     8341 cataatcatg gataaattgg ggcctaaact agccttttct ttgtttttaa tttgttcatc
     8401 cttatctgtc tatctacttg tgttagccca tagccagtgg agtttggtag cagccgctgt
     8461 gatagtcggc tactttatca atggcatgta tggtggatat ggcgcagtga ttagtagttt
     8521 gtatccaaca gaaatccgcg cgacggcgaa taatttcatt atgaaccttg gacgagctgt
     8581 tggtggtttt tcttctatcg tcatcggttt cttgatggat cattatagtt taactgccgt
     8641 aatcttgttt ttgagcggta tttacctagt cagtctgctg gtcttgacaa ctcttactgg
     8701 tgttacagca ttaagggaaa gcttgcagca ttgatagttg agtctgtaaa tgacaagaaa
     8761 gaggtgtggc aaaagcttgc atctgcggta ataagaccag ccaaatcaaa aacatgagac
     8821 actgtttccg tttggctggt cgtattactt gatgcagggc agctttttca actgctaatt
     8881 tccttctatc tggcggcgac tattctttga cgtccgtcat ttcaaagtac tttactaata
     8941 acttagcata acgaaaaact gtggatacac tcgtatattg agcgtctgca taatcttcca
     9001 aatttttctc aggatgttga tacatgaatt caatcaaatg ataattatat gattttctgt
     9061 agaaatgaag ataaataatg tagattggga agttctcctg cttttccata gaaaagcgcc
     9121 gattattctc attcttcaaa atgaccttat caggatattt ttggaacaat tcttctaaat
     9181 ctgttagata attcaagata gacttacttg ataccttcaa ttcgttttgt agttcttcta
     9241 ttgaacacca acgttctgtt tcaagcagta aatgaatcat tttaattttc ttttgagaaa
     9301 cttttgttaa aaaagtctgc atattttctc ctcctgtatc tactttacta tactacatac
     9361 aagaaaaaaa acggatacaa atatcatatt gtcacaccgt caatagtgca taaacctgtt
     9421 attatttctc tttttttcct aagtattcta ttgatatgaa tcatttaatc gtttattata
     9481 taaatgtcaa taatctaata ataaaataac gtacatattt tattattgca tacttaataa
     9541 cagaactata gcaaaaaatc ccgtgtatca acacgggatt agattaagtt tcctcaatca
     9601 ctattcataa cacaattatt ataacaagta agtaaacaat tgaaaaggac atttattgaa
     9661 aacggttgtt ttttacttaa ggaggaaatt tagcgaaaaa attagttgca ttgtttttgg
     9721 tcgcaggaag tttgttgagt gggtgtggtg ctactacgtc tactactcaa agcacaagtt
     9781 cagatcaggc ttccagcagc agtaccacga tctccgaaag tacggcagat tcatcgaaag
     9841 aacaagaaca aattgaaaag aaaaaagaag agaaacaaaa agcggaaaaa gctgcactag
     9901 aaaaaaagtg acagaagcgg aatctgcttt gtcacaagct gaagcaaatc ctacaaaaga
     9961 aaactatgca actgcttcag cagctatcca agcgctacct gaaaacaaac aagagttgac
    10021 aagccgtctt gctgcaattg attccacaat caaagcaaaa gaagcagaag aagaacggaa
    10081 aaagcaagaa gaaattgcac gacaacaagc agaacaagcg caacaagaag caatcgcaca
    10141 gcaacaagca gaacaggcga ggcaagaagc aatcgcacag caacaagcag aacaggcgcg
    10201 acagcaatca gcagctgaac aggcctctca gcaggcacaa gccgcagctc ctgagcaaaa
    10261 taatgaacaa actgtctatg tcacaccgac cggttcaaaa taccatacac ataaatgtgg
    10321 aaatggtacg tatacccctg ccactttaag cgaagctcaa gctcgtggtt taaccccttg
    10381 ctccaaatgt tatggaaact aattcaaaag ttatactata ataataaaat tcttctgaaa
    10441 cataaaaaaa tagaagatgg cgttccagat agtaaaccaa aaataacagt ttacacgtcc
    10501 atttcgtcag cttctctctt ttttcattta gtctgttccc tctgcttttt tggacatctt
    10561 cattcattct ttctcaatca aatagtacac ctctttttct tttcgcagtt tatatcttcc
    10621 ttttttcaat ttcattgaaa actgtttcga atgtttatgt tttacatgga ttttaagcag
    10681 gagctcctca tgttgcaagc gatagttcct gccttttaaa tcagaaaatc tcactagtcc
    10741 gtattcatca gaagctttca caaatggaac tttttctcta gtaatatagt gtttgccgtt
    10801 gagcgttttt acagaaaaga ccattccttc gattggtcgt ccgtttgtgt tctttacata
    10861 aaaagataaa ggatagtttc gcttagcgat ggccaaagaa tctaaataag cgatgagtag
    10921 ccaaactaag ccgattgatc caagtagtat accgataaac caaataaaaa atttccattt
    10981 tttccattga tcaattcctt ggatcgcttt ttttgcctct tttgcttgat aaggaatacg
    11041 atgccccgtc acaagcagtc gatgtgtatt gaccatataa ggagtacagg taagcaaagt
    11101 cacgagatct ctgccttttt ggatacccaa agaatctact tcatcaggta agaccacttg
    11161 gattttttct acttgatacg ccaatgtctt tccattgatc tggatgaaaa actggtctcc
    11221 ttttttcaac ttaggcaaat ctgtaaataa ttttgcttct gggagtccac gatgcccaga
    11281 aagaacggca tgggtgtttt tcccgccagt cggataagaa ctttcttcca ataatgacgt
    11341 tcctttttgc aaaaatattt ccgttgtctg atcgaatata ggtaagctga cattgatttt
    11401 tgggatttcg atgactccaa gcatatgttc cataaaaaag gcattactag gtaaatcttt
    11461 taatgctttt gcatcaactg cttggttgaa actgcctatc cctggtgata cgttttgctc
    11521 tgccaactgt tggtttttct ttgtcatttt ttcctgctgt ttttttattt cctctgaatt
    11581 cttattcgat gcttgctttt gataatggat gatcatttgc tgggcaagaa aatcattcag
    11641 tgaatcttct acaaaaggat aagaaaagat cccgatccca acgactaaaa tgattgccat
    11701 gaagatgtcc agtattctta ttctgctttt attcatcatg tccacctatt ttctaatatg
    11761 tatatgaaga ggttgtgaaa tcagcgcctt gacctgagta ttagcgaagc aaacggcaaa
    11821 atagttcctc ctattttgcc gtttgttgag cgaataccaa gagaccgacc acttgtcaca
    11881 acctttctcc ccatttgaat cactatcgtt tatttatcgt atgatttttg tttccaatag
    11941 tttaagcttc tgtttcttga tggcgttttt tgaaatagat cactgctcct ccaactaaag
    12001 cagtacctac caagatgaat acgatgatcc ccataccacc agttgaaggc aagattcctt
    12061 taggttggtt atcgatcgtt gtttcatcag aagttgttgg atcatcatct actgtaaatt
    12121 cagtgtacgc agcttcattc ttaggtaatg cgtagccttc tggtgctttt gtttcttgaa
    12181 ggcagtatgc cccttgtttc agcccttcga ttgaaaattt cccttctgaa tcagaaatca
    12241 atttcacttc ttcccagtca gaacctgtga cttcatctga ccaagcatat gggtattctc
    12301 ctgaggcaag gtttgctatt tttttcatta atttaacagt tggatctttt gctcttagaa
    12361 tagaaaattc tgcatcggct aatttttctt gcgtgttcac atcttgtttg atgaagtgga
    12421 aatcaccgta gttttggaca ttttctacag taatcgtgtt atccgtagtt gtattttccc
    12481 gattgactac accgtttgga ttcccaagat tggcttcatc tgcgccatgg atgataccgg
    12541 ttgtttgttg caattttccg ttttttacta taaaagtagt ttctttttgt ggattggtta
    12601 attgataacc atctggtgct tttgtttcaa ctaaagtata attgccgtct attaatccat
    12661 tgacactgat ggatccttca gtatcagttg tgaatgtcaa cgctgactca gcaggatcat
    12721 agagagcgac accagtgttt tcatcaaaac cggtcacgaa ttgattctct gaatttttga
    12781 tctggaattg cgcccctgct aacttatcta cccctgaaaa gttcccagct ttctggattt
    12841 tgatattgct ttctttgatg acatttttag catataggtg aatcgtgtct aaaggtttgt
    12901 cagggtctag ggtaagctta acacgatatt tggcgacgtt tcgacaccgg gctttggtgt
    12961 ttctacaaag acgtatacag catctttccc atcagattta cctggaagag tcaggttagc
    13021 taatccttca ccagaagtcg ttacacttgc gaagggtgta gcgtctgctg gaactgctgt
    13081 gtctttactt ggatagtacg tagcagtgat gtcatatacg ttgaattcaa cgccgttcaa
    13141 cggttcgcta ttcggaaatg tcaattccgt tccgttgttt atttgatttt ctggtgctga
    13201 agtgaatttc aatttatgca aattaaccgt aacattttct ggtgtaggtg atacggctgc
    13261 agaaacctct gcagttccta ggccgagaac gaaaggaagc agcatgatag cagttcctaa
    13321 aaacgtccat atttttgttt tgatgttttt cattttattt ccctcctatt gtttcctttt
    13381 tgttgttttt tataaaacca agctgtccct gttgaaagga ctagcaataa ccctaaaatt
    13441 gagaaacctc caagcatctc ttcgcttgtt tttggaaagc ttcctccatt tttttcattt
    13501 gatggaccat ttgtctgcat ctgaactggc gtttttccat tattcgtcgg atttgctttt
    13561 ggttcgttga tgatttccaa tggtatagga tgaagcggat cataactgcc tttttcaact
    13621 tcaaagagaa tggttgattg actcaatata tatcctttag gcggtttaat ctccactagt
    13681 tcgtaacttc caaaagacaa tccatggaca gaaaatgttc catctttact ggaagtaaaa
    13741 atcgttgctc ttgaatcatc tttggctacc caatgattta tcccgttttc ttgcgctaag
    13801 tattggtcat tttttttgac tagaaattga gcatctgcta gtttttgttt cgtttgccga
    13861 tccactttga caaattgttt gcctcctgtt tggatggcaa gatgtgtttc tactgtttga
    13921 ttgtcagtcg tcaaacgtac atcgttatca aatgtcgttt tttcactgtt ttctttcaat
    13981 gtcatttggt aacggatcgt taattttttt ccagcaaacg aactaagatc ttttccagaa
    14041 tctgataaga caaaaccatg ttttattttt tgtgtgtgaa atgtatgaat cttgtctcca
    14101 ccaatcaaaa aatcgatcgt ttctggttct agccataact gaggatcagc tgtatctttg
    14161 atcgtatagg ttcctatctc gttgatgttc tcaggaattg ttacggtgat gagataaggg
    14221 actttatccc catctgcaaa atctgtctgc ttattctcta ctgtttttgt taatgtgagt
    14281 gcagcaggta ttttttgttc gctctttgga tacaagtgga tggtcgtcaa tttatgatta
    14341 gatgaatctg tcacaggaag cacaacgaca aatggtgctg atttttcttt tacttaatct
    14401 gaagtcttta tttccgtaaa tcggtaaaca gcgtcacgac cttgctcatc tttgtccgat
    14461 gcactaaaag aagcaatgcc ctcttcctcg tctactgttt ttgtcacatt ttctgccagt
    14521 attttttcac tatcactttt ttgtgctagt tttctttgtg cgtcttccac actacttcct
    14581 tctgaccgca gcttgtaaaa ttcatccgag acatcatata tagcgaaagt aacgccgttc
    14641 aaccctttta ctccttgaaa gttatccggg atatttattc cgtcgttttg actgatactt
    14701 ggcatcttat cgctttcgaa cattcgatta tgcaaagtaa atgtaatagg tgattctcta
    14761 gctgacacag tcggagaacc tataaaaaag accagtagga taaaaaagag aacagcaaat
    14821 atatccacac gtacattttt tgtttttttc atcgctgttc acctgctttt ttcttacgat
    14881 ggataaaata aaagaagcca gaaaaaataa agatcagtcc taaaaagaca aagggcagta
    14941 gacctatccc acctgtcgaa ggaagcaaga gagtcggatt gatcgtcaac ttagcggtat
    15001 ctgttgcgat aatcgtataa ttattgttta cacttccttc gcctaaacta atcgttacta
    15061 tttgtgatcc aatatctgtc cggctgttaa ctgttagggt ataatccaat gattcgccta
    15121 agacaacacc ggtaacgtgg agatttccaa ccgaaatcga gtgactttgg gcatcataat
    15181 taaaggcagc gtcatcgaca gtaatcactg cgttagcttt atagatggta taagttcctg
    15241 atgtgttggc tatatcagta aatgacaact tgggatttgc tgctttgact ttgtctattc
    15301 cgctattcgt tagtttaatt gtatagctgc cagcattaac catatttttt gctgattgtg
    15361 catctgctgt gtaatccata ctgctaagtg ccacttgtac tggagtaagc gaactagtga
    15421 caccctctgc tagcttgaca tggattgacg ggacacctgc ttttccatca tatgtcttac
    15481 tgccactgag ctctaccgca ccgctgattt gcttaatcgt atagacatca gatgtctgca
    15541 cgatagtaaa taaatagttt gttctggcag ctagtttagc tagtccgctt tttgtcaatt
    15601 gccaagacgt ataggttcca atttcagtag gcgcagttac ttcaactccc ttttcattca
    15661 tcgctttgaa atcagttgga ttccatgtga tttgatcaat actgagatca ttgtttaaaa
    15721 gggatgttga ttctgtttta tcattaaagg catatttaac gatcggtaca acagaaagac
    15781 catcatagta cttactgcca tttgcattga ttgatactgt tgcatttgtc ttataatata
    15841 ggatgacttc agaaatctgt tttccatttg cgtcctttac gattacattt ccatttgtat
    15901 ctaaactagc taaaacactc attgctgatt ggttctttgt ttctatcgct gaaacagctg
    15961 taaaggtata tccgttgata gtaggcgtat tagtcgtcag gtcttgtgca gcacttccgt
    16021 aagtaagatt cattatttta gaaggagcaa tcatctgtcc aaaatagtct tgatacttaa
    16081 tagtgattgg tgtttgtttg cctgtatagg tcaccgtatc gacgttactt tctagggctg
    16141 tgaaagaacc gtttgtttgt gcaaaagaat agcccgttac tgctgttgga gtgataacca
    16201 ctttgtcacc cacatggatt ccagataatg tagtcccgta cgttccgatt tttgctgctc
    16261 caccattgac tgtataagaa aaagttcctg tccgattcgc attgacctga accgttgctg
    16321 cttgataggt tggaaaattt gaaacatttc ccttaccatc aacgatctgc ccatttttga
    16381 tcgagtaccc cgtgaatgga atatcatttg ctaactgtat accgattgaa aaagtactca
    16441 tacctcctcc ggcaggaagc cctccccatg ttgagggtgt aaaagaataa cttttatccg
    16501 cattgggtgt aactgaatcg atcgtatttc cagcggtagc cacccccata tagatacttt
    16561 ttgggaatgt gatagtagta ccatttactg tggagccaat aaacagatta tggatatctg
    16621 gacggttatc aataaaagcg attgctttgg ttacgccttg taacgcagtt ttatccgcac
    16681 tcatcgatcc gttaaccgca acaatgactc catctttggc atatatcact gtatatccat
    16741 ctggtatggt atgtccgttg aaattcgcag tggccgcaac agtactaaat gtctggcttc
    16801 tacctgtttc tgaataagct ttctcccctg ctgcttttgc tgctgtgatt tctttttctg
    16861 tcggatcaat cagttgagtc gaggatgtgg ttgaggttgt ctcgttgttg ctagactgat
    16921 tggtgctttc tatggactgt atttcagaat agggtggact atttgtctcc tctgtcgttt
    16981 ggttagcaaa atttgacgtt tctgtataaa cgtttacata aatacttgat aagctcccta
    17041 agaaagtaaa aatcaagatg atcgaaatca taatgggatg taagtatttt cttttagata
    17101 cagctttgag tctagaaaca aaatgttttt gacatatacg ttggatttta ttttgagttt
    17161 tcttaatcat tttcatccct cctctcaata aaaaatcatc aaataatcct ttacacgtta
    17221 ttctaaattt aatttttatt attttattga tgtatagaaa gtttggggct ttttttctct
    17281 ttttattcta aggttatttt agtcttgtcc tttaatacaa agattttatt tttaaataaa
    17341 aaatactctt tttttacgat tattgaaaaa aaaagataaa acttgattaa ttgaagtatt
    17401 gcttaaattt ggaagctatg agggaagaat aaaagagtta ggacgggatt atctactatt
    17461 tttctttgtt ttaaatattt gttatctttg aacataaaag agaaatgtgt cgactgcagt
    17521 gttgtgtccc tcatcacgaa taaacggtgt tcaactagct gatcttgtat tcacttaaaa
    17581 aatggtaaag tacgagttgc tattccccat tttcaatttt ctaacgctca ggacaaaata
    17641 ctgttttcac aacctcattg gaataaaagc atatatattg tttcaagaac gaaatcttgt
    17701 tatcttttta aagggcatct gtccaaaatc taattttagg aggtacgttt tggtatttat
    17761 tcgtacaaat aaaagagaat tatctttatt catgtaacgc atccaattga ttttttcatg
    17821 tacgctcctg gaacgatttt actgacgatt gcttatttaa ctgcgaatcg ctcattagct
    17881 tttatcatag ctatccatat tttgaataac gttcttggct ttgtattata gatacagatg
    17941 acaaaaaaag ttcaagtatg cggtgtagaa ccaatatcgc atacttgaac ttttttcaat
    18001 gagtataaat taaaacttct aattttcaat tcccatcatc tgatatgccc gaccgtctga
    18061 acttcttttg aaaaagccaa atgtgattag ataacgtctt tgttcgacat aatcgccact
    18121 agaaaatacg ttcagcagta tcgaattaac ctctgtttct gtataagtca ctccatggtc
    18181 aaagcgtttc aagacttctt tcaataggat gtatttcttt tttgttgaac ttggtacttg
    18241 ttttactttt ccatcattca tgaatgtgtc taaaactttc atcttttctt gctccgtata
    18301 agtataaacc atcttctttc tccttttgat tctaagaata ctccatttat gttagaatat
    18361 tctggatttt ttattaaagc ggttactctt aagaaagata ttatcatagt gctctttttt
    18421 ttactactac ttataggtag atttattcta atccattatt ctcccctacc aataattaaa
    18481 gaaaaaactg gatcagggtg ttcaaattac atgctaagaa aaaagaaccc ttgaatacca
    18541 agggttcttt ctcagtctga taacaatgat taacgacgga tttctttgat acttgtgaaa
    18601 agtcgttaaa gtattgatat aaaaggattt ttattacctc tgactacata ttgactaatt
    18661 tgttaaaaaa tcgtttttca agaatcctgt aataccagtg ctttcaccgt ttttttaact
    18721 ctctaaagaa ctaaaagcaa tcagaggttt taccttttca tttgacgtta agtggcgata
    18781 aattttcgaa gttgtttggt cagtatggcc taagcgatct ttcaagactt cccagctaca
    18841 gtgttccgct actaagcttg ccattgtatg acgagctaaa tgagtatgaa atggcttatt
    18901 aattcctgag cgattcacgc accgtttgac gtgatcattt acatactctc gacgataagg
    18961 aacaccacgt ttactagaat caagatacac aaacaaatat gtttcttcta taataaacct
    19021 gtaatgttgc ctcatctcac ccaaagcctt aatcaactgt tgtagctttt ccataactag
    19081 aggagttact agaactcgtc tagcagaact aactgtctta gtagtttcaa ttcgaaaatc
    19141 cttactctta cctttaactg ccgtttgctt atagaacaga atttcatttc ttttgaaatc
    19201 tatatctcca ggctgtagag caagcgcctc gctgactcgg cagccagtgt aaaatagaac
    19261 atggaaaagt acagacatcg gtaattcctc atgaacaatg ccataattca aaaaagttct
    19321 caattcatcg acagtaagaa acttgtcctc aatattattt atggcttctt ttaaatcagt
    19381 taccgttaac tgatattttg gcattctcac agacttcatt ggattttcac ttatcaaatt
    19441 ctttctttga caatatttaa aaatcatatt tagaacttga ataagtgatt gtaaactatt
    19501 tttggcatag ccaacatttg ttccattaac gccacattcc tttaaggtaa aaagcaattc
    19561 ttgaaggcac tctggtgtca aatcagacaa ctttttatca ccaatccatc gaacaataat
    19621 ttttaattgt tcctctcgct tatcataggt tatttccttt tgttcattta acgaacggta
    19681 atattggaac cattgctcac ctacaacact aaacttgtca ttgcttgcaa caattttttt
    19741 cttaagcctt tgttgtttct ccatttccag ataagcgagt tgcgcctcac gttttgaatt
    19801 aaagccacgt cttgttgttc gatatgcttg aaagtaccat gtgatcctac cagtagaact
    19861 gatttgcttc ttgaatcgat ctgttttttc catttattga ttcctcctat tttgaaggaa
    19921 cccaacaagc agaaaccctt tggaaatctt tctatctatg tcatcattgt accgcatctt
    19981 agaaagaaaa tgtaacttat aaagatcgaa gtagtcgcaa tttttctcga tagtcattcg
    20041 aatcactctt tctcatccat tctaaaacat catcacgaaa atagtgtcct cgaatcccgt
    20101 accaacaggg aggaaactca ccaagtttta ctaaacggtt cagtgtacta tcacttatct
    20161 gaaatagtcg ctttaaatca gtatttgtta ataccagagg taaatgactg actgcaagca
    20221 atttttccgt gtaatcttcc agtttttttt gtaaaattga atagaactct atttccagag
    20281 aattaacatt tgttttaact tccaactcca tcacccttct ctctcaaaat caaaacgacc
    20341 accacggtac cccttaaaat tgtaaaaaaa cactccagta ctccatcaaa acggactaga
    20401 aaagaggaat ttttaatatg aaacaaggaa ttgcagacat aaaaataatt aaagaaattc
    20461 ttgaaaaatc tacggctaat gcaatagcat tcggaacagg aatcaacttg agtacagtaa
    20521 aaaaacttaa aagtggagag agagctgaag aaaaattaaa tcttgccgat gctattaaaa
    20581 tcacagaatt tggcatgaaa aatatgccaa ccaaaatcga aatatggaaa tagtagtttt
    20641 tctgatctat cagctgtttt gccaaagcac ataggaatct cacctccccc cagtaatggc
    20701 gggccgattt ggttacggaa gtatcattat cgtagtcgcg attctcatat taaaatttgt
    20761 cgcttaaaag ctaaccttgt catggcgtaa ctagagaatc aaaatctaca tgtactgata
    20821 gatttgctat tcatttgtca aagagcgcta ggctattttc agcctatgga aatcctttat
    20881 tttgacccga taaaaaactt ccatagaatg aaaaggaaag ggaaaaaata ctttaacgag
    20941 ttgtatcaaa ttcaagtatc ttctcaatca attttgtttc cagttgtcta cgcatatatt
    21001 catctacata cataaccatt cctttttctt cttcataaac tggtttcatg gctaaccgtg
    21061 aaatataatt ttgatatttt gacagaacga tggacatggc aattgaatca ccagaagctg
    21121 cggctttgat tgtcgttagt ggtatgaatg gtatatatcg tttattttgc ttcatatttg
    21181 acacccaaat actttcctag ctcttccaaa gccatttgtc gttggtacca aacactgccg
    21241 agacttttcc caataatatc gccaatttca cgatcattaa gaccgatgaa gaaggacata
    21301 aaaatgattt cttgtctttg actaggtaat gctgcaatcg catttgccaa ctcctcattg
    21361 gaaataaaga ttttcatacc aaggacaata aaagtaccac tactattcaa atttggttcc
    21421 aaataataga actctgtacc taaccctttt tgttccaata tttccattga tatttgccgt
    21481 tggcgaaatc tagcgtactt cttttgaata tttaacgcct cattgcagac tactttctta
    21541 caaaaactgt caaacgtatg ttgaatgtgg gtttcctctg agtcgcccat aatattctcc
    21601 ccctcaaaaa attcattcaa ctcgttttac ttctatccct ttccattatt actacaatct
    21661 agga
//
LOCUS       DACDUU010000051        21423 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.051, whole genome shotgun sequence.
ACCESSION   DACDUU010000051 DACDUU010000000
VERSION     DACDUU010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.051"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            578..1348
                     /locus_tag="IWL30_001881"
     CDS             578..1348
                     /locus_tag="IWL30_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAP9706635.1"
                     /translation="MIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQE
                     ISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLK
                     IKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFS
                     FFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIER
                     EPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            1352..1990
                     /locus_tag="IWL30_001882"
     CDS             1352..1990
                     /locus_tag="IWL30_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9706636.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            2018..2446
                     /locus_tag="IWL30_001883"
     CDS             2018..2446
                     /locus_tag="IWL30_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706637.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            2903..3814
                     /locus_tag="IWL30_001884"
     CDS             2903..3814
                     /locus_tag="IWL30_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9706638.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            4031..4468
                     /locus_tag="IWL30_001885"
     CDS             4031..4468
                     /locus_tag="IWL30_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706639.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            4563..5447
                     /locus_tag="IWL30_001886"
     CDS             4563..5447
                     /locus_tag="IWL30_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP9706640.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            5671..8838
                     /locus_tag="IWL30_001887"
     CDS             5671..8838
                     /locus_tag="IWL30_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706641.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            8858..9403
                     /locus_tag="IWL30_001888"
     CDS             8858..9403
                     /locus_tag="IWL30_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HAP9706642.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            9431..9886
                     /locus_tag="IWL30_001889"
     CDS             9431..9886
                     /locus_tag="IWL30_001889"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HAP9706643.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            9883..10641
                     /locus_tag="IWL30_001890"
     CDS             9883..10641
                     /locus_tag="IWL30_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP9706644.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(10727..11467)
                     /locus_tag="IWL30_001891"
     CDS             complement(10727..11467)
                     /locus_tag="IWL30_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9706645.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            11528..12781
                     /locus_tag="IWL30_001892"
     CDS             11528..12781
                     /locus_tag="IWL30_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9706646.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLIGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            13103..13330
                     /locus_tag="IWL30_001893"
     CDS             13103..13330
                     /locus_tag="IWL30_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP9706647.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            13453..13779
                     /locus_tag="IWL30_001894"
     CDS             13453..13779
                     /locus_tag="IWL30_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP9706648.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            13847..14560
                     /locus_tag="IWL30_001895"
     CDS             13847..14560
                     /locus_tag="IWL30_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP9706649.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            14870..15721
                     /locus_tag="IWL30_001896"
     CDS             14870..15721
                     /locus_tag="IWL30_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9706650.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            15842..17707
                     /locus_tag="IWL30_001897"
     CDS             15842..17707
                     /locus_tag="IWL30_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9706651.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            17721..19163
                     /locus_tag="IWL30_001898"
     CDS             17721..19163
                     /locus_tag="IWL30_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9706652.1"
                     /translation="MQSRFSEKFLWGGATAANQLEGAYDRDGKGLSVADAMPGGKQRF
                     AIIGSEEFDWTIDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPN
                     GDEGTPNEAGLEFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYE
                     RFAQTVLERYSSKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVA
                     SSKAVKIGHELRSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGE
                     YPAYTARTHKKYGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSE
                     GNLLGGVKNPFLEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDG
                     TIIDDYRIDYLRRHIEAMANAVADGVDLMGYTPWGCMDLVSASTGEMSKRYGFIYVDL
                     DDEGNGTLNRSKKKSFDWYKEVIASNGVNL"
     gene            19271..19972
                     /locus_tag="IWL30_001899"
     CDS             19271..19972
                     /locus_tag="IWL30_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9706653.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQEILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            20144..20623
                     /locus_tag="IWL30_001900"
                     /pseudo
     CDS             20144..20623
                     /locus_tag="IWL30_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            20634..21140
                     /locus_tag="IWL30_001901"
     CDS             20634..21140
                     /locus_tag="IWL30_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HAP9706654.1"
                     /translation="MDYKKRVQQMFLDSGVVLKETELERIEYTDFGLGAIETEGLNLF
                     VYENNERYCGKELVLLPYQTCPEHRHPPRDENEGKKETFRCRKGLVYLYVEGKETKKL
                     SCIIPKGQERYYTAKKEIILHAGDQYTIEQNIKHWFQAGATGAVITEFSSSSDDPSDS
                     FTNPAIYR"
BASE COUNT         7245 a         3510 c         4497 g         6171 t
ORIGIN      
        1 cttttcatta catcaagtat tgtggtgttt attggggaaa aagaaaatca ttattacatt
       61 cataattgca ggaatcattg gtggttttat ttttatgtat tataaggagc aatttcttta
      121 tttgttccag atgtacgttt tcttgttcag cggaatatat ggtatctctt tgatttctct
      181 tttattagtt gaaaaaatcg cttcgttaag agaagcgttc aactatatcc aatacattct
      241 ttttacattt ggattgttgt tttctcttct tttgaccgtt gccaatgtcg aattagcaac
      301 tatcgttttt tcttcttcac agttagctgt ttcccatgaa ccaccaaaag aaatcgctgg
      361 tgaggactat catgtatttt atccagtaac catcgggaaa aaccacgtgg attttattta
      421 ttccaatcgt tttgcatcag cagcagatca ggaattatat gaaactttga ataaaaacgg
      481 aagtatatta gtcaatgtat cagattactt gaatgaagaa aatgaacagt ttggcagagg
      541 gataaaaata aatctgaatt atcttaagaa atttcctttg attgatgtct ctggaaggtt
      601 gatccaaata accgaaaaag aaacgcaccc tgttatattg atccctgaac gatatagaca
      661 tacagattta aaaaatgacc tggcgcaaat aacagaatat tatcaagaaa tcagtgaaaa
      721 agaaccgaaa tttctattta taaaaaacaa tcaacccatt tatacattta ttccttctat
      781 cccctggatc gaacattatc cggttgttga aatactgaca ttgaaaaaca gtatgtattt
      841 ggaaagaaat attctcagcg gagaaatcta tccaccgtta aagatcaaga taggctcttt
      901 gtcgaaagaa aggttatttg agctgattga agaaagtcag ttacgtgata acttgcagtt
      961 atcatttccg tatactcaga ctgaagagat agctgtcaaa gtgttaagca gatcatttca
     1021 ttacttgata cagatatttt tactcacttt cttttctttc ttcttgttga gctatgtcat
     1081 gttatgcata tattttgtct acaattgtcg gaaaatcgct atctttcgtt caagtggtta
     1141 ttcgctcttt gaaacgtata aagatttctt catgatgaac ctcatcaaat ggggaacgac
     1201 aagtgtaatc ttcctttttc ttatagaaag agagcctaaa tatcttttta atattttttt
     1261 ctttagcttc attgatttta ttctttcagt tgtttttatt ttgtctacag aaaagaaaag
     1321 tcagttattg ctgatgaatg gaggttaaca attggagccg ttgatagtga taaaaggtgc
     1381 gaaaaaaatc attcgaaaca aaacgattct tcatttggat cgtttcactg cggatgctgg
     1441 ggatcttgtg actgttgttg gagaaagtgg agcaggtaaa agtacgttat tgaatatatt
     1501 aggattgaat gatcagttca gttcaggaca attctatttg ttcgatcaag agacgaaaca
     1561 attaactggg agagagaagc gcttgataaa aagaaatacc atatcttttc tttttcaaga
     1621 ttttgggttg attgaatatg aaacaatcga ttttaattta gatattggat tgaagttttc
     1681 gaatttaaat gtttcagata agcgaacagc aaagtatgag gcattgaaaa aagtaaatat
     1741 aaacaagaag ctaagtacgc ctatctacac attatctggt ggtgagaagc aaagaatcgc
     1801 ctgtgcgaga gtcttattga agaatagcaa aatcatttta gccgatgaac cgactgggtc
     1861 attggataaa agaaataagg ataaagtagt tgaaatctta tcgcaacaag cttcccaagg
     1921 tacgttagtt atcgtagtca cgcatgatcc atatctgacc aaaataggaa acaagaagat
     1981 catgctttaa aaatcaaaaa ggaaaggatg tcgttttatg aaaaaagcag ttattatttg
     2041 tgcactaggg ataggttttg ttggaggaaa cgctatgtca tctgaagtgg aagcaaattc
     2101 agttcacggt gaaatctatt acaaatcttt aaagccagga gagattcatt ttgatgctaa
     2161 aggtaatatt attaagtcaa atttaccagt agaccgggaa atttctagaa cagcaattaa
     2221 tcatagctct ggaagatggg tttattatag tgatacaact tgtacgagaa aaacaggtca
     2281 ttctaaccac tatagtcgtg tctataatcg tcacggtgca aaagcgaaag ttggaaactc
     2341 tattcgatca gcaaatgcaa caaaaggagt atgggcctat gcaagtgcga caggaaatag
     2401 aactgacaca ttctcatgtt ggtacaatcc aaccggttgg tattaagtat ccaaaagttt
     2461 atataaaata gaaaaaacat ataatgaaac agcgttaaaa gttatttttg gtagttttaa
     2521 tatattcatt tttagttgta taaaaatata tcataataaa aacaacaaaa aaatgtctgg
     2581 aaaatcgaac ataaaagtgt ctgatttgaa tatcccagca gcggaagaag aaaaacaaaa
     2641 tcgataacat ttgttctaag aattagttac gttaaatacg gaacgcaaga tagacttgaa
     2701 aaacggtaac aaaagaagta gtatattaag gtgtgatgtg ctacttgaca tttattctgc
     2761 tgagcgaact ttctggattg tgtagcctta tatctattca ggacgtatcg agcagattag
     2821 taaaggtgtt cttgatgatt ttcatcttga acacctttgt ttttagcaat tcgcaaaaaa
     2881 cgaaaagtag gagaattgat aaatgaaaaa aagattttta gcacttgcca tcgtactagg
     2941 cactggctta ttgtcaggct gtacgaatgc cggtgaaaaa acagccgtta gttataaagg
     3001 cggtacaatc agcgaacaag aagtaatgga tagcttgaaa aaaatgcaag gagcagattc
     3061 cgctgtgcaa cagttgatcg tttatcaagt atttgaagat aaatatggag atgacgtatc
     3121 aaccaaagaa atcgattctc aatatgatca aacaaagaaa caattgggtg attcatttga
     3181 ttcacaattg aaatctgcag gttacacaga acaaacattc aaagacagta tcaaacaaag
     3241 tctagccttt caagaaggtc taaagaaaca tatcaaattg accgatgaag atttaaaaac
     3301 ggcttgggaa tctttccatc cagaagtgga agctcaaatt atccaagtag caagtgaaga
     3361 tgatgcaaaa gatgtgaaga aagctgctga taagggagac gatttctcta aattagccaa
     3421 agacaagtca actgatacta cgacaaaaga agatggcgga aaagtgaaat ttgattccac
     3481 tactacaacg gttccggcag aagtcaaaga agcagccttt aagttgaaag atggtcaagt
     3541 ttctgatgtg atcacttcta ccaatgcttc aacttatact acggaatatt atgtggttaa
     3601 aatggtgaaa aaccaaaaca aaggcaacga catggataaa tacaaaaaag aattgaaaga
     3661 aattgcaaca gacacaaaat taagtgacag taccttccag aataaagtaa ttggcgaagt
     3721 attgaaagac gctaatgtaa aaatcaaaga caaagacttt gaaaatgttt tgagtacatt
     3781 cacttcagat tctagtacag caagtagtaa ataagcggta taaataaaaa atatgaacta
     3841 tttaaatcaa taaaactgtt catctaaatc tttttattag aactaaatca acagtagttt
     3901 aattactact tgatttagtt ctttttattt taaaaatttt ttttacttag aatccagcga
     3961 ccctagatat ttttctgtat aatcttaaat aaagaaaaca atatcaaaaa atgcacgtag
     4021 aagtagataa atggagtggt tttgcaaatt tcttttattt ctagaagtta ttcacgtata
     4081 cggatttgtt tttctcacaa ataaaaaggc aggaagtaca atggatcatt tgtatataaa
     4141 atatattcat caattttccg ttttggtgtt aggcacacta attatagcgg ggattgtcgc
     4201 atttattatt caaggccgtg acatattaac agtacatcaa gagtttttca gttatgcatt
     4261 tggtattcct ctgatcgcta taaggtctga taacaaaaag tggaagaaac gctattacat
     4321 tctcttgcta tttttgtgtt tttatctgcc acctaagatt tttactttat taggtataca
     4381 atgcctatca ttttctttta tttcctatat cattgtttta attttcctca gtttcatagt
     4441 atggatgttt tatcttgtca aaaaataaat gaatgtttca tgaacagtac ttcatcacag
     4501 cctaattttt ttctttatgc tatcgttata ttaaagaaat caatcagaag ggaaagaaag
     4561 tgatgaaaaa agttttatta gtggtaaacc ccagttcagg cggtgaacaa gcaaaggaat
     4621 ttgagcaatt agcgatagca aaattggaat cggtgtttga tgaagtggtc gtcttgcata
     4681 caaaaaaagc aggggatgca aaaaacttta ctcgcgaagc ggctacggaa gggtatcata
     4741 gtgtgtttgt catgggcgga gatggaacag tcaacgaagg aatcagtgga atcgctgaac
     4801 aagaacatcg gccaaatttc ggatttttcc cattgggtac ggtgaatgac ttggctcgag
     4861 cattagggat accgctagaa cctgaggaag cgatcaatca tttttcgatt gaatcagtca
     4921 agccattgga tattggaaaa ataaatgacg actattttat gaatgtcgtc gcaattggct
     4981 ctatccctga atcaatcaat gatgtagatg cggagaagaa gacgaaattt ggaaaactag
     5041 cttatttcat gtcaggaatc aaacagttag cttccacaca atcctactcg tttcacgtag
     5101 aagttgatgg aaagaaggaa gaaattgaga gcagtacctt gctgatagga ctaactaact
     5161 cagtcggcgg ttttgaaaca ttacttccaa atgcaaaagt ggatgatggt aagctccatt
     5221 tggtttattt aaaagattct tctttgatcg atacgcttaa aacattgcca gacctattaa
     5281 aaggagtaga tgaatctacg aaaaatctag cctaccttac ttgtgagcaa ttagttgtgt
     5341 cattagctga tgaggaagaa ttgacgacga atgttgatgg agatgaaggg gataaattac
     5401 ctgtgacaat caaagtattg ccctcacatt tacaggttta ttgttaaaat ggaggttgtg
     5461 accgaggagt tggcgggaga acaccgcttg ttcgggataa gggactgtga caagactttt
     5521 gtcacagcct tttttttgta caaaaaagaa atttacacct aatagaaaaa tttttccata
     5581 aaaatacata ttttaaatat gaaagtacaa ttggaaatag attaaaacga taaaataaaa
     5641 ttgtcaaaac gtcagagagg atggtggaga atgaatcaat gtgtaaaaaa gctgattgcg
     5701 tcgattttgt ttgtcttccc tctcttgatg gtgacatccg tttttgccga tgtagagaaa
     5761 acaaacgaat caacgcaatc agagaatcaa agttttgaca agactacttt ttcagaagaa
     5821 tcgattactt ctgaaactac agaatctcaa agtgaggctt ctactaataa aaggcaagaa
     5881 gaaaataaga caagtgagac ctcagaagta gctgaacaaa cgtctgatag cagtgaagcg
     5941 gcactggcaa gagctgtttc agcggatggg tacagcgaag ctgccacaac agaagaactg
     6001 gcgcagcttc tcgcagacga atcagtaact aagatccgac tgattcagcc tttgacttta
     6061 gacagggaat tagagatcaa acgagatatc gttattgatt ttggtggatt cgctcacaat
     6121 ttcggcacac accatattta tatcaatgag atacctcagt cgatcgagtt ccagaatttt
     6181 aaaggaacag ctgcacatcc aggtggattg acaccaaata tcgatggaaa tgccattatc
     6241 attgcttata tgaccgattt ttggggaagc agatatcatt ttacgggaga aataaaattt
     6301 accgggacac ttgatttgta tgatgggtcc aaattaggac tgatttacgc tcctcgtgca
     6361 actgtcacat tagatggtgt ttccggtgtg ttggatgtcc aaccagtgaa ggaaggactg
     6421 aatgccgctc cgggtaaagc ctatttttgt agaagctatc aattgaatgt gatcaatggt
     6481 agtcaacttt atggaccgta tctagggaaa ttctacggtt ttctagattc aggagatgag
     6541 actggtagtg gaaaaacagc gccaggaatc catatcttaa gcggttcaaa ggtctctctt
     6601 gattatgatc gggcagatgg cgttagcgat gaaggagagg cagtcgatac gctacctggt
     6661 aatgtcatgt ttgaagtcag tggttcggga agcgaatttt cggtaaatac aaagatcaat
     6721 attaccaatg acaacaatcg cggaatcatc caaatgagag gttcaggatc gaaagtcact
     6781 gtatcgaatg gtgggaaaat caccatcaat acagcaacta cgggcggatt tcgcttgcaa
     6841 ggagattcta gtcaaatcca tgtatcaagt ggtggacaaa tctcggtcaa aatggctgga
     6901 gatgacgatc aacccgggaa caatgggatg cgttttgtag gaaaaggcct ttctttgatc
     6961 gtagaaggag ccggatcagt tatcgacatc gataagcaga gtgggcgttc cagcgcagtt
     7021 cgttttgaga atggtacgca aaagctgaca gtaaccaatg gaggctcatt gagggtaaga
     7081 aatacaggtg atggcaaatc ttacgtgaac agactcaatc aaggaaatca agcgatccaa
     7141 ttttatgacg cttctggttt tctagaaagt ccaggttcag ctgattttac agtcactgga
     7201 gaaaaatcaa atatcgatat acgcgcagat tccggagcaa ctgtagatac aactggagat
     7261 attgatttaa attttacggc aggtgaaaaa acttatttag tcatggtcgg gaaaacaggg
     7321 gaagataaga ccggcatcat cagtggggat gtgttgaatg tggacttaga atctcctacc
     7381 tattttgatt tccaaaacaa acggattgga agcgagacca atggcatagg cggctgggta
     7441 ttccaaggaa atggagattc ttctttaaag atcgcccatt cagaaatcgc tttatggcga
     7501 aaaacaggcg caacattcga taactttgaa aaagacccgg attatttttc tgatatgaca
     7561 gacttatcga tcaatgggac cgacttaggc aatttcgtat caagccctag tgcaggttta
     7621 acaaatgaat ttgctcagac aggtgccgga atgaaaaatt ataatcgaat cagtgccaat
     7681 aatcagctcc cgatgattga atccttaaga gtgccaacga atgcagataa aaaaatctat
     7741 gggcatgtcg ttgttcccga aggtgtcgag gcaaagccaa gagatgcttg gacaggcgaa
     7801 gtagaagtga ctttacgtat tacttacgca gatacgacga aatcatctgt agaaattact
     7861 gctctcacgc aaggacaaac agatgaaaat gaaggggcag gatataaccc atggggagaa
     7921 ggcgaacaag gcggcttgtt cgaagttcaa gtacccaatg atgagagatt aaaaacggag
     7981 gatcaagtgc aagttgttgc tgctaaaaaa gtaagaggtg gccaagaagt gacagaactg
     8041 gactctgcca aaacgacagt cgatgtgatt cctcctgaac ctgctaattt aagtaccaca
     8101 ttgatcaatg cagcttctcg cacgatcaca ggtacaggtg aagcaggagc cttggtttca
     8161 ttgaaaaaaa atcaagctgt tttgtcagaa acaactattg atgatacagg ggaatttgtt
     8221 ctttctatcc cagaaaatca actttttgca ggagatgtat tagaaattgt attaaacgac
     8281 caagcaggag aagctactgc taagggtgtg cagaacaagc caagtaccaa cgatgaaatt
     8341 gggaatcaca atccaagtga tacgccagtt gtttatcatg atgccccgcc ttttgcacct
     8401 gctacgaaga tcgtagtaga aggtggtctc tcatttcttg ccccttccaa actcgatttt
     8461 ggtcagatca aagcaactgg tttgacacaa gaagcatttg gccaaatgga tatacaggaa
     8521 aaattatgga tttatgatca gcgagaaacg aaaaatccgt ggatcttaac gataaaatta
     8581 tctcagcctt tcacaaatga tgcaggattt tctatgcagg atgtcctata tttcaagaaa
     8641 ggagaggagt atcaattagt gaatgaagaa gaactggaaa ttgtcagagg acaaaattca
     8701 acgaatgtgc aagcagatta ttcagaatat ttaaataacg gtcgggcgtt taaacttgtc
     8761 ttaaccaaag aaaatcagat agctggtaat tactcagcag agataacatg gacattagca
     8821 gatgcgccga cagaataaag atcaaggagg atgattgatg aatcgaatat ggaataaaag
     8881 tcatcgaaac aactgctctt tacttatgat ccttctcctt ggattcatcc tttggcagac
     8941 acctgtccat gcctccgaat tcaattttgc ggtagatttt gaattaccgg ctaatcagat
     9001 tgataagaac aaaacatata taaatgtaag gatggagcct gggaaaacac aggaaatccc
     9061 ctacacggtc tttaaccgaa cagataaatc agtaacagtt gagacattga tccactccgc
     9121 tacgacgaat tcaaacggtg tgatcgaata tggagaatca aaaagtaaat ttgatgaaac
     9181 attaagttat gatatgaaaa acttagcaaa aacagtggga gaaataactg ttcctcctca
     9241 aagtgcggtc actgagacac tttcactgaa agcgccagaa aaagagctgg ctggtgtggt
     9301 cgcaggaggg atcacgtttc aagaaaaaaa agagaacgtt tctaacaaaa gtacagttgg
     9361 tgtcgtcaat cagttttctc aagtgaaagc aatcttgatt tgaaatagcg aaaaagagtc
     9421 ttccccagat ttgaaattaa aaaaagctta tgcggatcaa agcgatggaa gaaatgtgat
     9481 taaggcgaat cttcaaaatc caaatgcttc ttatatgttc ggcttgacta catcggttga
     9541 aatctacaaa aaagaaacaa aagagcctta cctttcaata aaaaaagaaa aaatagatgt
     9601 agcacccaat acaaattttg atttacttgt cccattagaa ggaaaaaaat tagctccggg
     9661 acattatcaa gcggttgtga agggtgcttg gaaagaaaaa cgctggaatc tccataccga
     9721 ttttgaaata actgatgatg cagcggacca attgaatgaa aaggatattg atttaaaaaa
     9781 tcagccagaa acatttgact ggcgatggct gatacttgtt ggagtcattc ttattggctt
     9841 atttgctgga ttatttattt taaaagaaag aaggaaaaag aaatgaaaaa aacaattgca
     9901 ttaagtaccg ttctagctgg aagtctatta ttatgtacac aaagtgtttt tgctgagaat
     9961 aagagctcag ccactacaga aggaacgatt tctgtgacca aaccagatgc tgcaaatcct
    10021 gaagacgttg ttccagttcc tcctgttgat ccaaatgatc ctgatactcc ttttacacct
    10081 gacgaaccaa atcagggaac atccggatta ctgacgattg accaagctcc agatcttgat
    10141 tttggaacaa tcaagttagg tacagcaaaa acagtatatg ctgcattaca aagcggaacg
    10201 gacagtactg gagcacaaac acaagtacag aatttcgtac aagtaacgga taaaagcgga
    10261 aattatgccg gttggaaatt atcagtcaaa cgaacagaat ttattggtac aacatctgat
    10321 acgaataaac taactggttc aaaggtgaaa ttcaaaaacg ccgtgattcg aaatagcgca
    10381 actaatagca gtggaatgcc atctaccgtt tctggaactg gcgctgaagg aatcgaaatc
    10441 ccagcagatc aggcagtaga tctagtaact gcaggagcaa atgaagggat tggcacatgg
    10501 atttattcat taggagagaa tgtggaacaa gggagacaat cagtggaatt atttgttcct
    10561 gtatccaatt atgctgtaga caactacgtt tcaacattca catggtcgtt aacagatgct
    10621 cctacagaag aggaagcgta aaatcatatc aacgacttca gggagtggag ccgattcaag
    10681 gcccactccc tgaaatctgc tatgcttacg tctcagctct tattcatcaa ctcgaagcag
    10741 ttttttggaa gtcatggaaa cgttcctttg tcttttcaaa gatctttcga ccaacgagta
    10801 aggagtcacc gttgaaaaat tctaacacat aatgagcaga atcaattttc tgcaattgcg
    10861 ataagttgat acagtaagat tttaaaagta aaaagtaagc atacttttct aactctgtct
    10921 tcaattgttt taaagaagta ttcactaatt tatcttctgt agttgttttg accataactt
    10981 ggtttttcaa acctttgata ctttcaatat ataaaatttc ccgaggatac aggacgagac
    11041 ttccaccttt ttgatcaata aagattttat cgacatttac gatctgctgt tctttccaat
    11101 tgatctgttc tagcacttga cgaatttgtt cagataaatt cctgtctttt ctgatatatc
    11161 caatcggaag gattttccga ttgatgatct ctactgcaaa attcgtataa gcggtgaaaa
    11221 aaacaatatt actatgtgca ttttttttac gtatttgttc ggcgacatca atacctgtga
    11281 gtgattgctt caagtcgata tcgataaaga aaacatcgtg attatcaata gtgcccaact
    11341 ggatcaactg attgatttga gagatagaag tgagctgcac gatattctcg atatgatgat
    11401 tgaccaattc tctgatgatc cattcgctgt gaaactggtt atcttctatg ataaaatact
    11461 ttttcatatg caggctccta ttaaaaaaat atgtgtacat tttgaaacaa aacaatggag
    11521 gaataagatg gtcgcaacag tgacacttta tttgaccttt actttgattt tagatttagg
    11581 tatctattta ttcttttcga aacaactgct agtaaaaaac agctttggtt gtatagcttt
    11641 gatggtggtg acacttttac ttcctatccc taaagtgagc tttccttacc taattggctt
    11701 attattgatg tttctttttt attttactac gaaaaacctg tttatcagct gtttgatcag
    11761 taatcttttt tttgctatca ctggactcag cttttttctg gtttttgatc tgatggatta
    11821 tttaggaaag agacatcttg gaacagcaag tttttttgca ctacttatcg catttatttt
    11881 ctatctatta gtaaaaagta tagacaaaca gacgagattt ctcaacttgc tttttactta
    11941 tccaactgaa cactattttg gaggtatttg tattttatta agctggggcg tgatttactt
    12001 ggtattggga agtgttcact tccgctcatc taattatctg cttctatctc ttttgtcgat
    12061 agtcatcagt tttttttctt tgtttttttc cattatgttg atcagtttcc aagtaaaaga
    12121 aagacaacat caggaatcgc ttcaactgta tcaaataaat gaagaatatt accatcatct
    12181 agaggaattc aaacatgatt ataaaagtct actgttttca ttaaaaatta ctcttcagga
    12241 aaataaaaaa gaaacgttgg aatttttaga aatattagaa gatcattctg cagctatttt
    12301 tgaaaaaccg caaatacaac agctaacaaa tattgtatcc ccagctgtca ggggtgtctt
    12361 tttaaaattt ctagaaaaag cagaatcgga gaaaatcccg atccaagtag ttattcctgt
    12421 cgaagtgaaa acaatcccga ttgatctggt tgtttttatt cgctgtttat ctatttttat
    12481 ttctaacgca ttcgatcatc gtgtagccga tcagagtccg attacgatca atctgctgga
    12541 agaaaaagag ggatcacgtt ttagtatagc gaacaaggca aatccaacga atttgacgct
    12601 atcggagatg gtcaaaagag gaaaaacctc taaaaaaaat ggcgggttag gtctatacag
    12661 tgcgaaaaaa atgttggatg tttacgaaaa tgcagaacta aaaatcgaat tttcaaaaca
    12721 gaatcactat ttttttgtag aaatgtatct ggcatctaga aagagcaaat cagcctactg
    12781 acttactgtc aaaggataag tttgaggaaa gtctacacgg agatagagac aacaacacag
    12841 catattcaaa agaaacagaa gcgtgatgac cgttgtcacc cttctgtttc ttttgctatg
    12901 tagttaaaca aagtagttga cacttccgct acttagtatt agtacgaaag tatacttcat
    12961 gatactaagt agataagaag tgaaaagcgg aacccacgat cgtcaaaatg ctaactgctt
    13021 tgcttacacc agataaaggg aagagcaata tcgcttattt taggcgtgga gaagcaagca
    13081 aatccctacg atcacaaaaa aaatgctgac ccagcagtta agagagttag aacaagatga
    13141 aagtatccat cgaaaagcct ataaccaagt cccgcctaaa gtagagtact cgctcaccga
    13201 agaaggaaaa tcattaagag agattctagt agcgttgtct gtttgggaag aagaacgtat
    13261 cgaaaaacaa aaagcctcaa gggaagaaga cacgctactg catacacatg gtggctacct
    13321 aaattattga ttttctatga catttatttt aaataaaaga aaacaggagt ttttttaaaa
    13381 gcatcatcta ttttttatgt aaaaaaatgg tatgattaat caagtaagaa gtgattggaa
    13441 gaaggcaagc aaatgaatag tatggaaaaa gtcaaccagt ttagagatga acgtaattgg
    13501 cgacaatttc ataacgaaaa agatttggct atctctattt ctttggaagc atctgaattg
    13561 ttagaacttt ttcaatggaa aacgccggaa gaagtaaaag aaacacaatt agaacgaatc
    13621 aaagaagaac tggcagatgt attgatctat tcatacatga tggcagacaa ccttcacctg
    13681 gatctagatg agatcattga agaaaaattg atcaaaaata atttgaaata tcctgtagaa
    13741 aaaagcgcag gtgtacgaaa aaaatacaca gagttgtaga aatgtgtcat tcggctgttg
    13801 gaaagagcaa aagaaaaaca gtaaaatgag tctggaggga gagaaaatga aaaaaaaagg
    13861 aaaagcaaaa agatggctag tcaatatcct cttgttcctc ttgttattag taggactagc
    13921 tttgattttc aatgaacaga tcaaggatta ttttgtcaga gagacaggag ataaatatgc
    13981 tatagcgaat gtaacaaaag aagatctgaa aaagaataac gataaagatg tcagttttga
    14041 ctttgatgcg gtagaaccaa tgacgacaga aggggtcatg aggtcgcaaa tgagaggaac
    14101 agatctacca gtgattgcta gtatcgcagt cccttcagtt tcaatcaatt taccggtttt
    14161 caaaggcttg gataatacgt cactacttta cggagcagga acattatctc cagatcaaga
    14221 aatgggaaaa ggaaattatg ccttagcaag ccatcgtgca acgaaccctg aattgctgtt
    14281 tacaccgcta gaaaatttag agatgggtgc caagatctat ttgacagatt tagaaaatgt
    14341 gtatacatac aaaacatttt ttaaagaaaa agttgctcct acagataccc aattactgaa
    14401 tgaagtcgag ggaaaagaaa ttgtcacact aattacttgt ggcgacatgg acgccgtcac
    14461 gcgactagtt gtacaaggag aactggaaag tgttacatcg ataaaagatg cgacagatga
    14521 tatgagatct gctttcaatc tagagacaaa aacattttag gatactggga cataagtcgt
    14581 tacagattaa aaacagccaa aggatatgaa tcgataagga aaaaccaatt tgctgaaaaa
    14641 aagagaaacg caaagaggtg catctctgat ttggttgttt tctagataga cttgtgttcc
    14701 agttttttta ttttttatat gaaaaggctt gcataaataa aaattcgatg gtatgatgat
    14761 tttgaataag aaatggattg ttactgtacg gcaggcaaaa ccagaattca gctaagaaac
    14821 agtaggcgaa ttctaggttt ttttattttt cttgagaggg cgaagcaaaa tgaaaattga
    14881 taaaattttg aataacaatg tcgtcatttc gaaaaatgga ttcggggaag aagtcgtttg
    14941 tatgggaaaa ggactagctt ttcaaaaaaa gattggggac gagatttcgc cagaagcggt
    15001 ccaaaaagaa tttgttttaa gagattcttt tgctaaaaac caattccagc agctgatggc
    15061 tgatattccg gcagaggaga tcgaatgggt caaacaagta gtcgaactgg ctgaagataa
    15121 actaaaagtt gaattttcgc ctaatattta tctgacattg accgatcatc ttcattatgc
    15181 gatcacacga gcaaaagaaa atatccaact gcctaatcca ttattatttg aaacaaaaaa
    15241 attttatccg aaagaatatc aagcagcgaa agaggctctt ttgttcgtga aacaaaagac
    15301 gggtgtggaa ctttctgagg atgaagccgg ttttattgcc tttcattttg tgaatagcca
    15361 acaaggaaac gaagacatgc aagtgacgat gaccgctacc acaatggtaa gagatatact
    15421 gagtatcatc agtaaattct tcggcatcgt gttcgatgaa gaatctttga attatcagcg
    15481 tatcattact catctgcagt tttttgccca gcgttatctc aaaggggaag gatctgacga
    15541 gcaagatgaa tttctctacg cattagttca aggaaagtat ccaaaagcgt ttcattgtgt
    15601 cgaacggatc aatgagtatc ttcgacaaac gcaaaaacag gaaatgggga tcgcagagca
    15661 gatttattta acgattcata ttcaaagagt agtaagtgag aaaaaaagca tccaatcata
    15721 aggattgtta ctgtatggca ggcaaaacct ggattgaaaa aataggttca cgaatgtttg
    15781 tgactgtttt tttcaatctg ggtttttatt ttttaaataa aaaagaaaga aggaaaaaac
    15841 aatggataat caagcagttg gtcgtcgtgt atgggaagcg gtaggaggac aaaagaatgt
    15901 caaaagttta gtacattgtg ctacgcggtt gcgattcaga ttaaaagatg aatctttagc
    15961 agatacgcaa aagttaaaag aagacccgga cgtgatccaa gtcgtacaaa gtggcggtca
    16021 gtatcaagta gtgattggca gtaatgtagc agatgtttat caatcgatcg tggatgagga
    16081 aggtcttgca gtttcggagg atggaaaaga agagtccaaa aatattttga atcgtctgat
    16141 cgatattatc tctagtatat ttacaccttt tctaggggcg atggcagccg ccggggtatt
    16201 gaaaggattc ttatcattgg caacggtgat gggctggctg acagctgata gcggtactta
    16261 tcaaatattg tttgctgcag cagatggtgt ctttaccttc ttacctgtca tgcttgcttt
    16321 tacagcagct aaaaagttca aagccaacca gtttttagca gtagcaattg ctatggcact
    16381 tgtgtatcca gctatcacag cagttgctgg tgcgggagaa gctatcagtt tctttgggat
    16441 cccagtgatt ctttcaccat ctggttatac ttcttctgtc ataccaatca ttctagccgt
    16501 atgggttcaa agtaaattag agccattcgt aaaaaaagtg attccgcaat ttctgcaaat
    16561 gattttagtt ccgcttgtcg ttttagtagt gatggttcca ttaacatttc ttgctttagg
    16621 accaatcggg acggttgccg gtaatgcttt aggcggtctc ttcaactcga tttatggatt
    16681 tagtccaatc gtagcaggtc taatcatggg aagtttatgg caagtattcg tgatgttcgg
    16741 catgcattgg ggattcgtac cgatcatgtt tttgaatatc gaacaatatg ggtttgatgt
    16801 attgatgcca atgcttcttc cagccatctt ggcacaaggt ggggctgctt tagctgtagc
    16861 attgcgaaca aaagatacga aacttcgagc attagggatt tcttctacag tgacttcgtt
    16921 atttggaatc actgaaccaa ctgtgtatgg tgtgacatta cctttgaaga aaccattcat
    16981 tgccgcttgt atttctggag gtattggtgg agctattatc ggattttccg gagtaaaagc
    17041 tttctccagc agtttggtca gcttattgac gattccgact ttcattaata cggtggatgg
    17101 tgtagagtca aatgtgacag tagcagtaat agctacagga attgcttttg ttcttgcatt
    17161 cgtaggtaca ttgattttag gatttgacga acaaacacaa gacaatcaat tagaaaacaa
    17221 acatgcgaat gcaggagaac cgattacttc agcacggcat accttaaaaa gtcctttaac
    17281 gggtaaagta ctgccattga gtgaagtacc agaccaagtt ttttcttctg gcgtgatggg
    17341 aaaaggtatc gctattgatc cagaagtagg agaactagtt gcacctgcag acggagagat
    17401 cacgactatt tttccaactg gacatgctgt agggatcaca acgactgacg gagcggagat
    17461 tttgattcat atcgggatgg atacagtaga attaaatggg aatggctttg agatcttagt
    17521 caaacaaggg gatctcgtca aagcaggaga tttattgatt cgttttgaca tcgaagcaat
    17581 tagagcagct ggctatagcg taatcactcc agttgtcatt acgaatactg atgcatttgc
    17641 cgatatactt gaattagacc aaaaagagat tattgcaaac gaagatgttc tggcaattgt
    17701 aaaataagga ggaaatcagg atgcaatcac gtttttcaga aaaattttta tggggtggag
    17761 caactgctgc caatcaatta gaaggggctt atgatcgaga cggaaaaggg ctatccgttg
    17821 cagatgctat gcctggaggt aagcagcgct ttgcgattat cggcagtgaa gaatttgact
    17881 ggacgattga tcaagaaaaa tatatttacc cgaatcatcg aggaattgat cattacgatc
    17941 gtttcaaaga agatcttgcc ttgtttgcgg aaatgggatt caaatgttat cgtttttcga
    18001 ttgcctggac acgaattttt ccaaatggag acgaaggtac tcctaacgaa gcgggattag
    18061 aattttatga tcagctgatt gatgagtgct taaaatatga tattgaacct gtgatcacga
    18121 tttctcatta cgaaatgcct cttcatttgg caaaagaata cggtggctgg aaaaatcgaa
    18181 aactgatcga cttctatgaa cgatttgctc agacagtatt agagagatac agcagtaaag
    18241 tcaaatattg gatgactttc aatgaaatca attcggcctt tcactttcca gctttaagtc
    18301 aaggattagt aaaaagcaat ggagcaggag agtaccaaaa tattttccaa gcatggcata
    18361 atcaattcgt tgcaagtagc aaagccgtaa aaatcggcca cgaattgcgt tcggatatcc
    18421 aaatcggctg catgattatc tatgcaacaa cttacagcat cgatgccaat ccagtcaatc
    18481 aggcagcaac aatgatccaa aaccaagaat tcaatttttt ctgcacggat gtccaagtac
    18541 gtggagaata ccctgcatac acagcgcgta cccataaaaa atatggagtc gatccggaaa
    18601 aattagagca aacggaaaaa gacttccagt tgctaaaaga atatccagtg gactacatcg
    18661 gatttagtta ttatatgtct acggcaatca atgagactga tccacaagca gcaacttcag
    18721 aagggaactt gctgggagga gtaaaaaatc cgtttcttga ggcaagtgaa tggggctggc
    18781 agatcgatcc agaagggctg agaatcgcat tgaatgaatt atacaatcgt taccagaaac
    18841 cattattcat tgttgaaaac ggtcttggag ccattgatca tatggaagag gatggcacga
    18901 ttattgatga ttatcggatc gattatttac gtcgtcatat cgaagcgatg gctaatgcag
    18961 tagcagacgg tgtagatttg atgggctata cgccttgggg atgtatggat cttgtcagtg
    19021 catcaacagg agaaatgagt aaacgttacg gctttatcta tgtggatcta gacgatgaag
    19081 gaaatgggac actgaatcga tcaaagaaaa aatcttttga ttggtataaa gaagtaattg
    19141 cctcaaatgg cgtaaacttg taattaaagc agctgaatga aaaagaacac acttctcaca
    19201 tgtgatttgt gttctttttt tattttttat gtgctattct tttagtaata gtaaagcaaa
    19261 gggtgacgtt atgaaaagga cacgtgtact atacttggaa gtcgcagata aaattaaaga
    19321 agatattttc tcaggaaaat atccagtcgg ttcgatgtta ccgacagaaa gtgaattaga
    19381 agaactgttt caagtcagca aaatcactgt ccgaaaggca atcgaactgc tggcatcaga
    19441 tgaatatgtc gcaaagaaaa gtggtcgtgg gacgacggtt ctaagcaatc gtccttacaa
    19501 tcgattatcc aaagcggcaa gctttacgca aatcttggag aactcccgat atgaagtccg
    19561 gaaagaaacg ctggatttgc aattggtcca tataaagaag gaagatcctt tgttttcctt
    19621 tttcggtgag caagctgttt gttttaagcg cttatattat ttgaacaatc agccttatat
    19681 ttattttgtt cattatcttc cacaagaaat gatgcaattc agcagagaag aatttggcaa
    19741 agaatctttg tatcgtttgc tgacgaaaaa aggatatact attgactcat tttctgatga
    19801 tttctcggca gtatttttaa cagaagaaga acaagaaatt ctgaaaacag acgcacagat
    19861 cggcatcaaa cggatacgga aatcgtctac gcaaattggg gaaatgattg aatattcgga
    19921 ggcggtctac catacggagc tgcatcctta ccatattgat tatgaaacat aaataaaaaa
    19981 agaggttgtg aaaaagctgc cctgcatcaa gtaataagaa cagccaaaca aaaatagctt
    20041 ctcatattag cttctcatat ttttcgtttg gttaggctta ttaccgcaga tacccggctg
    20101 tttctggatt accatttatt ctggataagg gggtgtggca cgattttcag aaggaatcta
    20161 tgaaccaatc actgtattga ttttagcttc gatggtaacc ccattcggct tgacgatcgc
    20221 ttatttctta gggaaaatca ttcgtaaaaa tatattcaat cgtcaagaaa tcgatacatt
    20281 gaagaccgct tttcctatgg ggatctgtca gatcacagag ggatgtttcc ctatcgtatt
    20341 gaacgacttg gcacgcaatg tcatagcaac gggagtcggt ggggcagtcg gcggtggatt
    20401 gagcatgttt tggggcgcag atagccgtat tccggcatcg ggaatgtttg cagtcgctac
    20461 gatgaccaga ccatgggcat tcattggtgc attgttagct ggttcttttg ctacgggaat
    20521 aactatttta ttattgaaaa aaccagttga cccaaatgca gaagtcatcc aagaagaaaa
    20581 agaggaagaa gagatatctt gggatgactt gacaatcagt taggaggaac agaatggatt
    20641 acaagaaaag agtacaacaa atgttcctag attcaggagt agtattgaaa gaaacagaat
    20701 tagaaagaat cgaatataca gattttggat taggagcaat cgagactgaa ggattgaatt
    20761 tatttgttta tgaaaataat gagcgatact gtgggaaaga actggtatta ttgccttatc
    20821 aaacttgtcc agagcatcgg cacccacctc gagatgagaa tgaaggaaaa aaggaaacct
    20881 ttcgttgtcg aaaaggctta gtatatcttt atgtcgaagg aaaagaaaca aaaaaacttt
    20941 cctgtattat tccaaagggg caagaacgtt actatacggc aaaaaaagaa atcatacttc
    21001 acgctggaga tcagtacacg atcgaacaaa atattaaaca ctggttccag gcaggtgcaa
    21061 caggcgcggt tatcaccgaa ttttcttcca gcagtgatga tccaagtgat agttttacga
    21121 atccagctat ttatcgttaa tacaaaggtt tgggaacaag ctgatgaacc aaaatgatca
    21181 aatttcgatt atttggttcg tcggcttttt tatttagaga aacatcaggc gttggtgaaa
    21241 cataacacga gaataaaatg gatttttcat taataggaga aacaagtgtg tgactattta
    21301 aataatgcgt gttattgaca tgcaggacga aatcccaatt tcttctataa gtaagtcgtt
    21361 tcaattttat atttactagt tatttatttt tttggttact gtaaaatagc tgaaaaatta
    21421 ttt
//
LOCUS       DACDUU010000052        21363 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.052, whole genome shotgun sequence.
ACCESSION   DACDUU010000052 DACDUU010000000
VERSION     DACDUU010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.052"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            474..725
                     /locus_tag="IWL30_001902"
     CDS             474..725
                     /locus_tag="IWL30_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706655.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
     gene            759..1037
                     /locus_tag="IWL30_001903"
     CDS             759..1037
                     /locus_tag="IWL30_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706656.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            1634..1891
                     /locus_tag="IWL30_001904"
     CDS             1634..1891
                     /locus_tag="IWL30_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706657.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            2004..2615
                     /locus_tag="IWL30_001905"
     CDS             2004..2615
                     /locus_tag="IWL30_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706658.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            2794..3351
                     /locus_tag="IWL30_001906"
     CDS             2794..3351
                     /locus_tag="IWL30_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706659.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(4050..4571)
                     /locus_tag="IWL30_001907"
     CDS             complement(4050..4571)
                     /locus_tag="IWL30_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP9706660.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            4770..8462
                     /gene="nifJ"
                     /locus_tag="IWL30_001908"
     CDS             4770..8462
                     /gene="nifJ"
                     /locus_tag="IWL30_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAP9706661.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            8651..9538
                     /locus_tag="IWL30_001909"
     CDS             8651..9538
                     /locus_tag="IWL30_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAP9706662.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            9535..10704
                     /locus_tag="IWL30_001910"
     CDS             9535..10704
                     /locus_tag="IWL30_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706663.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            10728..12083
                     /locus_tag="IWL30_001911"
     CDS             10728..12083
                     /locus_tag="IWL30_001911"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAP9706664.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(12155..13960)
                     /gene="glmS"
                     /locus_tag="IWL30_001912"
     CDS             complement(12155..13960)
                     /gene="glmS"
                     /locus_tag="IWL30_001912"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAP9706665.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            14369..14659
                     /locus_tag="IWL30_001913"
     CDS             14369..14659
                     /locus_tag="IWL30_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706666.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            14672..15697
                     /gene="sppA"
                     /locus_tag="IWL30_001914"
     CDS             14672..15697
                     /gene="sppA"
                     /locus_tag="IWL30_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP9706667.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            15700..16359
                     /locus_tag="IWL30_001915"
     CDS             15700..16359
                     /locus_tag="IWL30_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP9706668.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            16516..17448
                     /locus_tag="IWL30_001916"
     CDS             16516..17448
                     /locus_tag="IWL30_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706669.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHQVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            17602..18891
                     /locus_tag="IWL30_001917"
     CDS             17602..18891
                     /locus_tag="IWL30_001917"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAP9706670.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            19138..20388
                     /gene="clpX"
                     /locus_tag="IWL30_001918"
     CDS             19138..20388
                     /gene="clpX"
                     /locus_tag="IWL30_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAP9706671.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            20471..21058
                     /locus_tag="IWL30_001919"
     CDS             20471..21058
                     /locus_tag="IWL30_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAP9706672.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
BASE COUNT         7082 a         3648 c         4603 g         6030 t
ORIGIN      
        1 acaaataatt ggtttattct ggcaggcgta atgctttttt ctagtttcta tttatttagt
       61 aatgctctat tagcacagcg agggaaatat aagtttaaaa tattagatta gatattattt
      121 ttagagggga taaatataaa atgaaaagag gttatttata atggaaaaag tattaccaat
      181 taagagctac tgtattccaa ggcttttcat catagaattt caaaagtaaa gacatgtgtt
      241 tcacaaatag tgtctatcct ggctaaataa ttcttccact atctgcttta atttcagtag
      301 acttttcggc aagttatttg ctaatgagat gaatagagat tacaactcgc ctataaccgc
      361 ttaaaacaag cttttttaaa gtatagttag caagtcgtat tcatatgttc aaagaggagg
      421 gcttatgaac tttgaattca ttccgtgtga acagaacagt agggggaatt aaaatgaaag
      481 aaaagcctat aaataaatgg tcattgtata aaaattcaat gtttggagtt gttatctttg
      541 gtgtttgtgc tggattattc cttttcatat tattacaaac ggtgactctt ttctcttcta
      601 atcctactta tggatggaga gacagtatag ccttagggat gtttgcggga ggatatggat
      661 tcttattgtt gttctcaatt ctcacactaa gtccaatcca tggactcatt caactttgga
      721 agtaagaacg agccttaaac ttttcgttct cggaagaaat ggaaaaacac aacataacag
      781 gcagtatgta tgtggatgat caatggttta tcagcactga tgaggtaaaa atagctgcca
      841 ttaggagaga ttacctgcag aaaattcata aaataaaaga tcctaatggg aaaaaggcaa
      901 ggttaattat aactttgata acatctaatg gtaagaagtt aaaacttgca ggatcatatg
      961 ataatgttaa aaagttcgaa cattggtatc tgcaaaaaga cagcattagt cttaataaac
     1021 aatttgaaaa aaattaatca aagtcatcgg caacacgaat aatttgacat cttgatattg
     1081 tgatataagc aaaaacggca tttctgagac cgaacagcat aaaaaattta aatgttggaa
     1141 tttatataag caaaacccaa gaagctagat gcgatagagc atttaatttc ttggggtttt
     1201 gcttgggaat cagcatatca attaaaatga tcatctcggt atttcaaaag tgggtacgat
     1261 tacaaatagt tggagcaaaa tagtgggtaa aattaccatt ccagtgtctg tcttgcctct
     1321 ttttcgtcaa tcacaaatag acgagattca taggaaaaat cgatacaggg tatgatttca
     1381 atgatattat gaccaattac aaaatagatt gataaagcat tttttgcatt tttataatag
     1441 tttattgata ataatgtatg agatacagta taatgattta aaaggtaacg tgttgtgacc
     1501 gaattaaatg aattaatgcc tattttttat attccttagt ggacaaaaat ggagacagtg
     1561 aagttaggag cattgtatcc tttgttgcgt agttcaaaaa agtaagcgat ttctataatg
     1621 gtcagggtaa acggtgaggg ctgtccacgt aaatcctact attgaaatca caatgatcgg
     1681 acattcaaaa ggtggtgctg aagcagcaaa ttcagtagct ataaatacga atgcgattat
     1741 atttaatcca gcgacagtaa atttatctgc ttatgaatta gactcaaaca catatacagc
     1801 aaacatggta gcttatgttg tcgagggaga tgcattaaat gttgtttttg gaggagtata
     1861 cacctattgg agaattaatc atgttagata gacaatatga tgcaccttag tggagaatga
     1921 tatctcgcat aggtaactca gtaaaaaatc aaggtatgga agcaattatc tctgcattgg
     1981 aagatcgata tagataggcg gatatgaata gtaatagggg gaataaaatg gaatacaatg
     2041 gatcaatgag aattgtacaa attatatttt ggattacatc gggaattgtg atcataggag
     2101 gagttttttt gatgttgcca agcttaatat ttcctttctt tgcattaatc tcacctaaaa
     2161 tacccgaacc ggaaatcacc tatggagaat ttccatttag gattgaatac gaactagatg
     2221 gacagattaa cataattgaa gatactgtca tagctgagtt taacgggtgt gagttcagcg
     2281 caggttctat gaaaagagaa cgaagatgga gaagtagatt agctagtggt agagaagatc
     2341 taccttttgg ggacgatcta ggaatctatt ttagtcgagg aagtgcgcaa tattatatgg
     2401 gggaaaatgt acaatcaatg tcattaaaac cacatatagc ccttagaaat ctcgaggaag
     2461 gttttaaaag agaggtagac tttatattag gagaaagtgg atacataaga actgtactta
     2521 atttcggaga agcgcaagaa gttttgacac aatacggaat taccttaatc aactgggaaa
     2581 tatctgaacc gattgttaac aattttggtg attagaaaac aattacgata aatagaaaca
     2641 agccaaatca tcagcaagta aaagctgatg atttggcctt tcttatacga gtagtaggaa
     2701 ttcatagcga atattattac gaccagtaga tattttgata ttaaaggagt attacgaact
     2761 aatatgtgag aatgtagagg tgatgtgcga ataatgagaa atttatcaaa tagaaacaaa
     2821 atattaatca ttattgttgt tatagctgtg tttcatctgg gaacgaatgc ggttttaagt
     2881 agaataatat tgggtcctaa gccaccaaga ccagaaatta cccgagggga gtttgatttt
     2941 agattagaat atgaagtaga tggggaaagg attgtgatag aagatacaat tgttgcatta
     3001 tttgatggat tttctgcaga tgcaggtagc atggcatggt atcgcacgtg gcgtctacat
     3061 ttagcaagtg atagacgatc acgaaatatc ttattagatg aattagagga tggtcgaaga
     3121 atatattatg tcccagaaag tgctaactat tctatgggag atgtacagaa aaaaagagag
     3181 cctaatccta attggtatcc atttaatggc gtaacaatag aatatccacg caataaaaca
     3241 cctgaaatag gtgcaaaatt tataagtggt ttagaagatc tgtacgatag gtttggaatc
     3301 cgtcttatta gttgggaaca cgatccacca atagaaaatc gctttgaata atcgaaatct
     3361 actaatccat ttcatcagcc agtgaatgtt gtaatggaac aatttggttt agtgttcaaa
     3421 accattgaaa tactaggctg tgatcagcaa aaatgaatct gaataaaaaa gagagcaact
     3481 attagctgag aggagttaat agttgttagc tttatacgaa tcaaagtggc aaatacagat
     3541 gcgtatgcat ttgtgtttgc catcttgcgt ttggtagacg agataggtat aagctaaaga
     3601 aacaacttgt ttaaaataaa cgacctagct caacaaatga ccatctaatc aactaacagc
     3661 atacataaaa gcaactattt ttgaagtaga attgaaatcg ggataactta aaaagtgatt
     3721 gggatactag tgagaatcac cgtacataaa tattactcgt taagggaaat tgagaaattt
     3781 tgtatgaaaa tttaagaatt agttagtatg cgcaagtcaa aaatatgaat aaattatttg
     3841 gtggagataa tttgcttagc ttgtcataag aaatgagata atctggcaga tgcaagcttt
     3901 tggacaccat ttattcggaa taaggaggct gggacagaag cgtttaactc caagaaataa
     3961 gaaggagttg cttctgcttc cgccgtttat acgtgtttag agcgtgagac aaaagtgttc
     4021 tttacttttg tcccacgctc cttatttttt tattttgttt ggatcaattc ttttaattcc
     4081 tctattgaaa tagattcatc aaagatttct ttacccaaga tgacagttgg aacaaatcga
     4141 atatgtgctt gctcagcttc gttgatgaca gcttggctca gctgttcgtc tttttgttct
     4201 gtcagcttta gtttatctac tgcaaaatcc gctacttctt ggagagaaag atgtttccac
     4261 tcgtcttgag tatcgaatat ttgtttgatc tctttgatcg ctttttggcc atcgctgatt
     4321 gtgagatacc ggtgcatcac gttccctcgc tgtaagcttt ctttttcttt atcgaacaat
     4381 ttgatcactc gctgcaattg cccgctttgg actgcttctt ctaatagctc ataagattct
     4441 tcaaaccatt gtcggcaata cggacaagct aagttgatga attcgaccat ttttttagga
     4501 gcattcgatg aaccataaag gattcctttt tgtgtatttg ttttcgttgc atcaatcact
     4561 gatatatcca tgttaaaaca cctctttcgc ttatagttta tacatgtctg acaaaaaaag
     4621 gcaacgattt agactgtttg tctaaaaatt agatgaccaa ttcaaataga tacatgtaaa
     4681 atatttttat aaataaaaga aaaaaatggc atgttttacg ttataatgga acagataata
     4741 agagagaata tgaacaagga tgatgaatca tgcgaaaaat gaaaacaatg gatggcaata
     4801 cggcagccgc atatatttct tatgcattta ccgagattgc tgcaatatat ccaataacac
     4861 ccagttcgac catggctgaa ttagtagatc aatggtcagc agcaggaaaa gaaaacgtat
     4921 tcggtcaacc agtaaaagta gtagaaatgc aatccgaagc aggagcagct ggtgtggtcc
     4981 atggatcatt gaaaactggt gcactgacaa ctacatatac cgcttcacaa gggctgcttt
     5041 tgatgatacc gaatatgtac aagattgctg gggaactact gccaagtgtt ttccacgttg
     5101 ctagtcgggc agtgacgaca aatgcgttga atattttcgg agatcacgga gacgtgatgg
     5161 ctgcaagaca aacaggattt gctatgttag ctgaaagcag tgtacaagaa gtgatggatt
     5221 tagcacctgt tgcccatctt gcttctattg aaggagaagt cccttttttg aatttctttg
     5281 atggattccg tacgagtcac gagattcaaa aaatcgaagt tttggaatat gaagaattag
     5341 ctcctctgat caatcaggag aaattagctt catttcgtaa acgagggatg aatcccaatc
     5401 atccgactgt tagtggtacg aaccagaatc cagacatcca ttttcaacaa cgggaaacga
     5461 tcaaccccta ttatgataaa ttacctgaaa tcgttcaaaa atatatgaaa gagatcaacc
     5521 gattacgagg aacttcttat gacttggtga cttattacgg agcagacgat gcggaagaag
     5581 tcattgtttc tatgggatca gttgcacaga cgattgaaca aacgattgat tacttgaatg
     5641 caaacggacg gaaaacaggt tttttgaata tccatcttta tcgtcctttt ccagtggaaa
     5701 cattcgtgca aaagttgcct catacagtaa aaacaattgc tgttcttgat cgaagtaaag
     5761 aaccaggggc aggtggagaa cctctcttat tagacgttca aagtgcgtta tatgattctg
     5821 aacttcgccc ggctgttatt ggtggtcgct acggattggg gtctaaagat gtgactcccg
     5881 accaaatctc agcagttttt gatgaattga aaaaagaccc aagcatacgc aaaaaacggt
     5941 ttaccatagg gatcgtggat gatgtgactt atcaatcttt ggaaccaaag gaaagtctgg
     6001 atttgacaga accgcaaaca ttccaggcca agttttgggg atttggatct gacggaacag
     6061 ttggagcaaa taaatcagct atcaagatta ttggagatca caccgataaa tatgcgcaag
     6121 gctattttta ttatgattcg aaaaaatctg gcggtctgac tgtctctcat cttcgttttg
     6181 gagatacgcc gatacgttca gcgtatcttg tcgagcatgc ggaccttgtt gcctgtcaca
     6241 caccagctta cctgcatagc tatgacttgg tcaaaggatt gaagccaggt ggcatttttt
     6301 tattgaatac tctttggagc gacgaacaat tagaaacgca tctgccctta aaattaaaac
     6361 gttacttggc tgaaaataac attagattct atacgatcaa tgccatgaga ttggcccaag
     6421 aagtcggttt gggacgccgg atcaatacgg caatggaaac ggcttttttt aaattagctg
     6481 acatcattcc ttttgatgag gtgcttccgc tgctaaaaga agaagccctg aaaagctatg
     6541 gacataaatc aatggctatc gtagaaaaaa acatacaagc aatcgatcgg acgaccgaac
     6601 tgcttcatca agtcgcgatc ccagaccatt ggagaacgtt ggagatgcct gaaaaaatca
     6661 ataaaaggaa cactggttat atacaagaaa tacttgaacc agtgaatgca caggaaggaa
     6721 atcaactctc tgttggtaca cttgtagcaa atggaatgac aactggcgaa atgcctttag
     6781 gaacggcttc tatggaaaag cggggcgtcg ctttagaagt acctgagtgg attagtgatc
     6841 gctgtacgat gtgtaatgaa tgtgcctttg tttgtcctca tgctgccatc cgtccatttt
     6901 tagcagatga agaggaaatg gaagaagcgc cagaaggatt tatcgtgaga gaaatgcgag
     6961 gcgcagacgg cgtaaaatac cgtatccaag tttcagtgga agactgtaca ggttgtggtc
     7021 tgtgtgtaga ggcttgtccc gcaaaagaaa aagctttagt gatgaagcct tatgaagaac
     7081 agaaagaaca agctgtgaat tgggcttttg caatgacatt aagacaaaaa gcaaatccag
     7141 tcaaaggaaa gaatactgtc ttgtctacac aattcaatca accgcttctt gaattctcgg
     7201 gcgcttgctc aggctgtggc gaaacacctt atgtaaaatt attgacacaa atgttcggtg
     7261 atcgaatgat gatcgcgaat gcgacaggct gttcttctat ttggggcggt gcttctcctg
     7321 cttctcctta tacgaccaat gaatgtggtc aagggcctgc atggtcaaat tctttattgg
     7381 aagataatgc tgaatttggt tatggtatgc ttctagcggc acagactcgt cgcgaggctt
     7441 tggcaagttc aatcaaagaa gtattgccaa aagtatcaga agaactacga gaattgatgg
     7501 aagactggtt agtacacatg tttgagagtg aaggaacgca gcaaagagca gcaaaactgc
     7561 aagctgcgat cgaaagcgaa cgatcaattt tccctgaact ggaggatata tacaagagaa
     7621 aggatctatt cgtaaaaaac agccaatgga tgattggtgg agacggatgg gcttacgaca
     7681 ttggttttgg cgggatcgat catgtacttg ccagcggagc agacgtgaat atccttgttt
     7741 tggataacga agtctattcc aatactggcg gtcaaacctc aaaagcaaca ccagcttcgg
     7801 caatcgctaa attttcggct agtggaaagt atgcttcaaa gaaagatcta ggaatgatgg
     7861 cgatgacata tggaaatgtt tatgtggcgc aaattgcttc aggtgccaat caaatgcaaa
     7921 cgatcaaggc atttgaagaa gcagaaaagt atcctggtcc ttctctgatt attgcctata
     7981 cgccttgtat cacacatggc ttgcttggcg gaatgaaaga atcgcttaaa gaagcaaaag
     8041 aagctgtttc ttctggttat tggtcattat atcgatataa ccctacaatg gtcgaaaaaa
     8101 acaagaaccc aatgactttg gactacaaaa aacctgattt ctctgctatg cctgatttca
     8161 tgaggaaaca agttcgcttt tcctcgctag ataacgcaaa tccaaatctt gcaaaagctt
     8221 tatttgaaaa gactgtccac gacgctaaaa cacgttttta caactatgct gcactgacgg
     8281 gagaattaga aaaaatcaag aaacgacttg aaccagagga atctatcgat gtacccgtta
     8341 agactagaaa agaaaaaacg acggaccttg aagcagaaga aagaagagct gctagaagag
     8401 ctgccagagc agaacgacgt aaaaaaatgg aacaagctac aaaagaaaaa aaggaaaaat
     8461 aacagaaaaa atataatgaa taattctctg aagtttaaaa atatttattg cagagaaaaa
     8521 taaaacgcat acttgcaatt ttcttgaaaa ttatgcgttt ttctcaagaa aaatacgagc
     8581 atatcagcct ctaaaattaa aacatatgat atactagaga ggaaatctca aaagagaaga
     8641 gaggcttttt atgtcatttc aaatcgggga actttttaat ttggactatt ggcagcaaat
     8701 gttttccggt gacttgttct ctctgaattt tttggtgaac atacttgata ttctggttgt
     8761 ctggtatctt gtatacaaac tgatccaact gctgcgaggc acaaaagcga ttcagcttct
     8821 gaaaggtgta gctattttta ttgttatccg aatcgtagcg gaaatcattg gtctgcacac
     8881 cttgtcttgg ctgatgaacc aagtcattac gtacggggtg atagctgcta tcgtgatttt
     8941 tcagccggaa atccggagag gactggaaca tcttggacgc agttcgcttt tcaaacaatc
     9001 aaaaagcgaa cagcaggaag atgagaagat gatcttgtct tttgacaaag ccatccaata
     9061 tatgtccaag cgaaaaatcg gtgcgttgat cactatcgaa cgtcatactg gattagatga
     9121 gtatattgag acagggattg ctttagatgc agatatcact ggggagctgc tgatcaatat
     9181 ctttattcct aatacaccgc tgcatgacgg tgcagttatt gtaaaagaag gaaagatcgc
     9241 agtagcaagc gcctatcttc ctctatcaga aagtatgttg atccctaaag aattcgggac
     9301 tcgacaccgg gcagctgtcg gtataagcga agtcagtgac gcgattacca tcgttgtctc
     9361 tgaagaaacc ggggacgtca gcatcacgtt agataatgaa ctgatggctg gcttatccca
     9421 gcaagaatat ttggcaatct tgcgtagaga actaattgct gaagagaaaa caaaaaacaa
     9481 gaaaaacgtg attcaatctt tcctagacgg tatgactaag ggaggggatc gcaaatgatc
     9541 tcaaaagaga aacggacaag cattctttac agttttattg cattgctgtt cagtattgcg
     9601 ctgtacttca atgcaaatgg ccagtccgtt ccaaatacgc tttctggaaa tgaagcgtat
     9661 aatcaaacag ttacgggggt gccgatcaag atttcttatg attcaaagcg ctactatatc
     9721 catggatatg aaaatgcagt gactgtcaaa ctttccagtg caaaccgagt tcagttgaat
     9781 gcagaggcaa acgaagatac acggatgttc cgcgtcacag cagatatcac taagctgggc
     9841 gaaggaacac atgaagttcc gctaaaaatt caaaatttga gcagtgctgt cacagcaaag
     9901 atcgaaccat cgactattac agtaacggtt gagaaaaagg taataaaaga tttcgatgtg
     9961 gaaacgttga ttccttcaac gttcacacca tctggatatg aacttgatga tacttccgtt
    10021 agtcctaaaa aagtctctat cactacaggt gacaaaacat tggcagaaat caaacgagta
    10081 gtagctgatg tggacgcttc gatggtcaca gatgatggga ttaatagtga ggtcccaata
    10141 caggcgctga atgcagcagg ggaagtatta agtatcgtct ctgatccagt gcaagtaaca
    10201 gtgagagctg attcgatcaa gccaagcaag tcggtacgac tttatgggat acagcaagga
    10261 acaccagctg ccggggtaga aagctatgat ttcagttttt caaaactgga agcagaagta
    10321 agcggctcta gcgatttgct tgcatcgatc ggtgacagta ttgctgtacc gatcaatgtc
    10381 tcaggtatca atcatcgaac aactcgtaaa attgaaatac cagtagaaga ggggttaagt
    10441 atcaagcccg aatcagttag tgtggagatt acacccgtgt tgcaaacgtc aagcagtagc
    10501 tctgcttcga cgagcagtcg gacacaagaa aatacaagta cgagtagaag tatttcatct
    10561 gctgattcag gaacggaacc atcgacaaca accatttctt ccagttcgga atctgggaca
    10621 agcagctcga ccacaagtac agaagaaaca tcagaaacaa gcgaatcaac acaaatgagc
    10681 agaacaaatt cctcagaaaa ttaagttaaa ggagtaattt tacaactatg ggtaaatatt
    10741 ttggaacaga cggcgtacgc ggtgaagcca acaaggaact tacgccagaa cttgcattta
    10801 agctgggacg gtgcggaggt tatgtcctaa gccagcatga agaagaagga aaacgcccac
    10861 gcgtgttagt agggcgggat acaagaatct caggtcaaat gttagaagaa gcgctgattt
    10921 caggactttt atcggtgggt atcgaagtgt tccagctagg tgtgatctct acaccaggtg
    10981 ttgcttatct gacacgtttg caaaaagcaa gcgcaggggt catgatttct gcttctcata
    11041 atccagcaca agataatgga atcaaatttt tcggtgcaga tggttttaaa ctagtagatg
    11101 accaagagct agaaattgaa gcattattag atgctgaagt ggatgaattg cctcgtccat
    11161 cagctgaagg attgggaact gtagaagaat ttccagaagg cttattgaaa tattctcaat
    11221 tccttgtaca aacgatcaac ggtgaccttt ccggtttgac catttgtgtc gatgcagcaa
    11281 acggagcaac ttcaacaagt gttaatcgtt tgtttgccga tcttgaaaca gatttttata
    11341 caatgggcac aaatccagac gggttaaata tcaatgacgg cgtaggctct acccatccag
    11401 aacgtttagc tgagatggtc gttgaaaaag gagcagatgc tggactagct ttcgatggcg
    11461 atggcgatcg tattattgcg gtggatgaac ttggaaacat tgtcgacggt gataaaatca
    11521 tgtttatctg tgctaaatac ttggcagaaa aaaatcgatt gaaaaaagat acgatcgtta
    11581 cgactgtcat gagtaactta ggattccaca aagcggtgga aggaatcggt ttgaaagatg
    11641 tcgttacaca agttggtgat cgttacgttg tagaagaaat gcgcaaaaac gattataact
    11701 ttggcggcga gcagtctggg cacatgattt tcttagattt taacacaact ggagatggga
    11761 tgctttcagg aattcagtta ctgaatatca tgaaacagac aggtaagaaa ttatcagagc
    11821 ttgcagcaga ggtaacaatc tatccgcaaa aattagtgaa tatccgcgta agtgataaaa
    11881 acggtgcgat ggatgttccg gcaatcaaaa aagtgattga agaggcagaa gctgaaatgg
    11941 gatcagaagg aagaatccta gttcgtcctt ccggtacaga accattactt cgagtgatgg
    12001 cagaagcgcc tactgatgag aaagttaatt actatgtcga taaaatcgcg gcagtagtca
    12061 aagaagaaat cggactggat taagagatat tttcataaaa aataaaagaa caaggaccag
    12121 tgtcagatgt ctgacactgg tccttgttct tttattattc tactgtcact gatttcgcta
    12181 aattacgtgg tttgtcaacg tcgaatcccc gtaatagcgt tgcgtaatat gcaagtaatt
    12241 gagtcggtac gactgtggtt agagtagtta gataagggtg gacagttggt agcaccagct
    12301 ggtcactttc cttttctaat ccctctgaga caataactag tgttttagcg ccacggcttt
    12361 cgacttcttt caggtttcct cgagtatgcg caccagtcac ttcttcactg atgattccga
    12421 tcactggtgt tccttcttcg atcaaagcaa tcgttccatg tttcaattcg ccagcggcaa
    12481 aaccttctgc ctggatataa ctgatttctt tcaatttcaa agcagcttca agagaaacgt
    12541 tgtagtcagc accacgtcca atgtaaaagg catttcttgt atcgctgaga tatacttctg
    12601 ccaactcttc taaataatct ttttgggcga tgaccgattc catcgcatta gcaactaaac
    12661 tcaattcatg agtgatatcg aatacaagag aagtttcaaa ttctttttta tcaccgatag
    12721 ctttggctaa taaagttagt acagctattt gtgcagtata agctttcgtt gatgctactg
    12781 cgatttcggg tccagcatgc aatagcagtg taaaggatgc ttcccgcgaa agagtagaac
    12841 cagcaacatt tgtaatcgta agtgaagggt agccgagacg gttgattttt accagcactt
    12901 ggcggctgtc tgctgtttcg ccactttgac tcaagaaaat aaagaatggt ttttttgaaa
    12961 gaagaggtgt gttatagcca aattcactgg ctaaatgaac ctcaacagga atctgtgtca
    13021 atttttccaa agtctgttta cctgctaacc ccgcatggta actagttccg caagcaacga
    13081 tatagattcg atcgctatca gacaatgtat cgaccagctt ttgatcaaga gagacacgat
    13141 tgtcttctgt tacgtatttt tgagcgatac gacgcatgat aataggctgt tcgtcgattt
    13201 ctttcagcat ataaaaagga taggtacctt tctcgatatc tgaagcatct acttgtgctt
    13261 cgaatgaaga acgttctacg atgtcacctg caaaattttg aataataact ttttctgatg
    13321 tgacggtaac catctcttca tcttcaatct caacgaattc cttcgtatga gcaagcatag
    13381 ccattgcatc gctggcaatc acattgaatc catcgccgag tccgattaat agtggacttt
    13441 tatttttagc gacgtaaata acatcaggtg ctgtccgatc aatcaaagcg aaagcgtaag
    13501 aacctttgat gacccgcaaa gcttttaaaa acgcctcttg cgttgtagta tccttcgcga
    13561 aagtttcaat tagatgagcg ataatctcag tatcggtctc accaataaag tggtcatttg
    13621 ctaaataagc aattttaagt tcttcaaagt tttcgattac gccgttgtga acgagcacga
    13681 agcgtccact ttgggaagta tgtggatggg cgttttcttc agaaggttgc ccgtgagttg
    13741 cccatcgtgt atgaccaata cctatatttc ctgtagtatc aatatttatt ttgtttttca
    13801 aattttgaat tctgccttgt gctttgacca gataatcagt tgtgccatct gagacaaaga
    13861 taccagctga atcataacca cggtattcta atttttctag accgtcaatc aatcctggtg
    13921 ttacattttt taatccaatc attccaacaa ttccgcacat aaaaaacatc ctactttcta
    13981 taatgaattc gtatgaatgc ttccagctgc aggaaaatcc ttttgtttta tctttgcaga
    14041 cattttttgt ggattttctg tagagtgcag tccctagggc catccgccga atctttcgat
    14101 aaaccctctc ctcgtcatct gtttacacag acctggcgct atcctaaaag gtataactat
    14161 cctccttttt gttttttatc tggaaacatt taaaatcata accttttgcg aatgttcttg
    14221 tcaacaaaaa aagaataaaa aataaattgg tatatatcaa attctttgtc aaatgtcctt
    14281 tttatgtcaa aaaataatgg gtttttatct atcatctttt tcaggagttc ggtacaatag
    14341 aaagtaatcg gatagaggag gagtaaaaat ggattcaaaa attgagatca tgacactggg
    14401 aatgttaaaa aaacaattgt ccaaattcga agcatcagct ggtgtatcag atgatacgaa
    14461 aatattttta gatactggct gggatagcat ccaagaaatc gcacctgatg ccttggaagt
    14521 tgctcaagct cgtgagttta cggtagaaga tgaattaaca aaagagtcgt tttccgggta
    14581 tgcgcgggaa gaaaaagcag agcggttcga cacttctgag cagtcagaaa ctgtgatcgt
    14641 cataaaaaac ttgtattaga aaggagaaag aatgaataaa agacgttggg cagcagttct
    14701 gattgctgca ggattatttg tggtttcgtt agtttcagct agtttgacta agcctcagga
    14761 aaacgatcaa attgagggat tgaacagttg gttatatggg gatgaagaac tagctccaaa
    14821 tgtgttggaa gagggagaca gtacgcaaaa aatcgtaaaa ttatcagtag atggaacgat
    14881 tgctgcggga gggtcttcag gtttatttgc ttctgaaggt tacaatcata aaaattttat
    14941 ggaacaactg aaggcgattg aagaagatcc aacagtcaaa ggtatctttt tagaagtaaa
    15001 ttctcctggt ggcggtgtct acgaaagtgc ggaaatcgct aagaaattgg atacgattcg
    15061 taaagaacat gatatcccaa tgtatgtcag catgaaaaac atggcagcta gcggtggcta
    15121 ctatatttct gctcaagcag ataaaatctt tgcaacagaa gaaacagtga ccggctcaat
    15181 tggtgtgatc atgtcaggat tgaattattc aggacttcta gaaaaactgg gtgtggaaga
    15241 tacaacagtt aaaagcggag cgttgaaaga tatgggttca ggaacgcgac cagagacaaa
    15301 agaagaagaa gcggtgcttc aagcatatat cgacagcgca tatagtcgtt ttgtagcaat
    15361 cgtcagcgag ggacggaata aatctgaaga agaagtcaaa aaaattgctg atggaaggat
    15421 ttatgatggt gtccaagcaa aagaagtagg tttagtcgat gaattgggat ttcctgaaga
    15481 tgctttaaaa gctatacgaa aggaacaaca gcttgaagat gcggaactag tagaatattc
    15541 ttccacgcca tctgggtttg ctagtacatg gttaggaagt aaactagcag aattccaggg
    15601 attgaagcct tctgagacta gtcagatctt atccgttctt gagaatctag ggacagcgga
    15661 atcaccaaaa gcaatgtatt attacggagg tgagtaagca tgtcagagct ttcaaatgaa
    15721 aataaggaac aagaaaaaga taagcaacag cagcctgctg taggacctga ctccaacttt
    15781 tcccaaaagg tactgagctc ttttgaaaga gagcctttga cgcccgaaga aatcaaaaag
    15841 aaacaacgta aatggaaaaa gtatacggac acaagaaatg aaccgccaaa agcaaatgat
    15901 tttcctaagt atttctatgc aggttttttt atccgaatgt ttgccttctt ggtcgatctt
    15961 ttgtgtatcg gagctattac gcgtttaaca cttggtattg ccaaaaatct aggttggctt
    16021 aatttttcag attcttatct gagtctctac ggattcacag ccttacttat ttatctagcg
    16081 tattttattt tattgacgaa attcaatcat ggccaaacga ttgggaaaat gatctttggt
    16141 attcgagtca tcagctttga tgaagaagaa cttagctggt caaccgtttt agttagagaa
    16201 ggtgcttgcc ggttcattct aaaatttcct ttgctgatgt tagggtatct tccagcagtc
    16261 tttaatcaga aaaagcaaca tctgggcgat tttttcacta atacaagtgt tgttacgatc
    16321 aatctgatca aagcgttcaa ccaggaagtc aacagctgat gagcaataaa aagcagtaac
    16381 agttgaaagc accttctaaa agtcagatct aaaatctagc ttttagaggg tgcttcagtt
    16441 atttttaatt gtcatgcttt ttttgttttg ctactattga acatgagctg aatatgagca
    16501 agaggaggac actgcgtgaa gccgattgat tttccagaaa aatatgagaa tttaatgcga
    16561 gtggcacaac aagcactagc aaaccagcaa tatcagcagg caaaagaatt atttcaacga
    16621 gcttacgagt tgaaagaaag ctttgaggct aatagcctgc ttgttttttg tctttacgag
    16681 ttggacgaga aaaaagaagc attgaagcaa gcactcttgc atgaaaaaca gtatcttcaa
    16741 aatgaggagt ttgctgaatt ttattttgac ttgttaatca gtacacaaga ttttttatat
    16801 gctagaaaat tgatagcaag tacagatttt tatgaagctt tcgagcagcg ggtcattgaa
    16861 aaaatccaat ttgcagaaga attatccgga caaatggaga ggcagaagat caaggcactc
    16921 cacaataaat cggaggaact tccttcgttg gaacctgcac gtcagcttgc gtctattgat
    16981 gagatcgaac agcttcctta tcatgagttt atccaaacag cgagtaaact tattgtatcg
    17041 ccagaggtcc atattttagc aagggcaaaa ttactggaga cacttaggca attgaacgaa
    17101 tgccatcaag ttttctattt gacgattgaa gaaaagttag tcaaagtcat accaaaagat
    17161 ctgccaaagc cgcagcagca ggcaagttac cagcaattat gtgtattttc agatcgttac
    17221 gggaatgaag atgcgctgct tagttccgtg ttaaaagaag aattcacgtt gcaatctgca
    17281 attgtttatc ctgtctatga tacatacatc gaagacccga agcgctggtt tcaactgact
    17341 gtagaggcgt gtacaggaaa aacgatgtat gaaggtacag aagatgaaaa acaagatttt
    17401 ttaaaaaaaa gagaaaaaat tttgcagcaa atgatcttct tccattagat tttatcagtg
    17461 aaaaagataa aaatgttatg aaaaacaagg attctatcca ttcaatatgt ttacaatttg
    17521 aaagggttag tgtactattt aaaggtatgc atgaaacgtt tttgatgtat aaaatgaatt
    17581 tctaaactcg gagggaatta aatgactgca acatttgaaa aaaaaggcac gaatgatggt
    17641 gttttaaaat ttacgattgc gcaagacgaa atccaaaaag gattaacaac tgcgtttaat
    17701 aaagtgaaaa aaagcttgaa cgtacctgga ttccgtaaag ggaaagtttc tcgtcaagta
    17761 ttcaaccgta tgtacggtga agaagcttta tatgaagatg cattgaatgc tgtattacca
    17821 gaagcttatg aagctgctgt caaagaagct ggcatcgatc ctgtttctca accaaaaatc
    17881 gatgttgaaa gcatggaaaa aggtcaagac tgggtaatca ctgctgaagt aacagtgaaa
    17941 ccagaagtaa aattaggaca atataaagat ctagttgttg aaaaacaaga ccgtgaagta
    18001 acagatgctg acgtggatgc acgtatcaaa cgtgaacaag aagcacaagc tgaattagtt
    18061 atcaaagaag atgctgcagc tgaaaatggc gatacagtag taatcgactt cgaaggcttt
    18121 gtagatggcg aagcgtttga tggcggaaaa ggcgaaaatt actcacttga attaggttca
    18181 ggctcattca ttccaggttt tgaagatcaa ttagttggtc ataaatcagg tgaaagtcta
    18241 gatgtaaatg taactttccc agaagattat caagcagaag atcttgctgg aaaagaagca
    18301 gtcttcaaag tgacaatcca tgaagtaaaa gcaaaagaag tgcctgaatt agatgacgaa
    18361 ttcgcaaaag atgttgatga cgaagtatca acattagacg aattgaaaga aaaatatcgc
    18421 aaagaattag tagaaacaaa agaaaaagca gcggacgatg caaaagatga agcagcgatc
    18481 cgtatggctg ttgaaaacgc agaaatcgtt gaattgccac acgtgatggt tcatgatgaa
    18541 gtacatcgtt caatggacga attcttgaac aacatgcaac gtcaaggtat ctctcctgaa
    18601 atgtactatc aattaactgg ttctacagaa gaagacttgc ataaacaatt cgaaggtgaa
    18661 gcagaaatgc gcacaagaac aaatcttgtg atcgaagcaa ttgcagctgc tgaaaatctt
    18721 caagcaacag aagaagaaat caatgcagaa atccaagaat tatcagaaac atacaacatg
    18781 ccaatcgaac aaatcaaacg cgtgttgaca gaagatatgc tggaacatga tgtgacaatg
    18841 aaaaaagcaa tcgaattgat cacaggtaca gcgacagaag cgaaagaata gaaatcaagt
    18901 aataaatcat ttctgaagaa gctacttcta gaaaattgtt tattcgaaat aagggactgt
    18961 gacacgattt tgtcacagtt ccttattttt tgttaagagt aaaaaatgca aaggtatagg
    19021 tcagttgatc ctttgttaaa cactttttct ttttactaaa ctatgttatg atagtaaaag
    19081 actgtaaagt ctttgatagt ttcttgcata ctttttgcaa gttaaggggt gaagaccatg
    19141 tatgacaatc caagtagcaa tgagaccgta cgctgttctt tttgcgggaa aacccaagaa
    19201 gaagtgaaaa agatcgtagc tggtccaggg gtctacatct gtaatgaatg tatcgaccta
    19261 tgtaaagaaa tcattgatga agaattttat gaagaagctg tacgcgaatt cactgaagtc
    19321 cctaaaccac tagaaattct tgaagtattg aataactacg tgattggaca agatcgcgca
    19381 aaacgctcgt tagctgttgc cgtatacaat cactataaac gtgtcaatca gcaagcacaa
    19441 gaaaacacag atgatgttga actgcaaaaa agcaatatct gcttgatcgg acctacaggg
    19501 tctggtaaaa catttttagc acaaacatta gcaaaaacat tgaatgtacc ttttgcaatc
    19561 gcagatgcga caagtttgac agaagctggt tatgtggggg aagatgtaga aaacattttg
    19621 cttaaattgc ttcaatctgc tgattacaat gtggaacttg cagaaaaagg gatcatctat
    19681 attgatgaga tcgataaaat tgcgcgtaaa agcgaaaatg tatctattac tcgtgacgtt
    19741 tccggcgaag gtgtccaaca agccttgctg aaaattctag aaggaactgt tgcaagtgtg
    19801 ccacctcagg gcggacgtaa acatccacat caagaattta ttcagattga tacgacgaac
    19861 atattgttca tcgtaggtgg tgcatttgat ggcatcgaaa cgattgtgaa gaatcgtatg
    19921 ggcgaaaaga cgattggatt tggtacacaa aaccaaaaac tttctgaaga cgaaagtgtc
    19981 atgcagcata tcattcctga agatttacta aaattcggat tgatccctga gtttatcgga
    20041 cgattacctg ttatggcagc tttagagaaa ttgacaacag atgatctggt acggatcttg
    20101 actgagccga aaaatgcgct agtcaaacaa tatcaaaaac ttctttctct agataatacc
    20161 gaacttgaat tcgaacctga agcgttgaga gccattgcga aaaaagcaat cgaacgcaat
    20221 accggtgctc gaggacttcg ttccatcatt gaagaaatca tgatggatgt gatgtttgat
    20281 atcccttcaa atgaaaacat cgaaaaagtg attatcacaa aagaagcagc tgaattgtct
    20341 ggtgagccaa cagtaatcta taaagattca gagaaaaaag cgggttgatg aaaagaggct
    20401 gccgtaaaag tcattttggc ttttgcgaca gccgcttgct ttattcatca ccttgttaag
    20461 gagagagacc atgaaagtcc atcaagctga aattatcata agtgcagtgg caccaaaaca
    20521 atatccagat acacaattgc cagaaatagc tctggcagga cgatcaaatg tagggaaatc
    20581 ttcttttatc aacacattga ttgatcgaaa gaatttagcc cgtacttccg gtaagccagg
    20641 aaagacccag acattgaatt tttatttgat cgagaacgca ttacattttg tcgatgttcc
    20701 gggatatggg tatgcaaaag tatccaaaac tgagcgagca aaatggggaa agatgatcga
    20761 gacatattta acgactagag accagctacg tgcggtcgtt tctttagtag atttgcgtca
    20821 taagccttct caagaagata tccagatgta tcaatttttg aagtattatg atatccctgt
    20881 aattgttgtt gccactaagg cagataagat ccctcgtggt aaatggaaca agcatgagag
    20941 cgaaatcaaa aaagcgttgg attttgatcg caatgacacg tttattctct tttcttccgt
    21001 tacaaaacag ggaaaagaag aagcgtgggc agcaatcgaa caagcaattg aatcataaaa
    21061 agaataaaaa agacttacca gtcaaactct ttcataaaag tttggctggt aagtcttttt
    21121 ttgagcttaa cttacttttg tttcttgtct tttttctttt cgttttgtgg ctgtggtttt
    21181 agaaatcgat cgatctcttc ttttggtgca actgtttttt ccggatcgac agcaaatgat
    21241 tcgaaaagtt tatctaaatt atctgtacgt tcaaatttca accccattgt aaacacctct
    21301 ttgtttcttt ttacttagtc tatcatgtaa tcgtccaaat tggtttctaa gacataaagt
    21361 ttt
//
LOCUS       DACDUU010000053        21195 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.053, whole genome shotgun sequence.
ACCESSION   DACDUU010000053 DACDUU010000000
VERSION     DACDUU010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.053"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            611..1939
                     /locus_tag="IWL30_001920"
     CDS             611..1939
                     /locus_tag="IWL30_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP9706673.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2141..2974
                     /locus_tag="IWL30_001921"
     CDS             2141..2974
                     /locus_tag="IWL30_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAP9706674.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2990..3727
                     /locus_tag="IWL30_001922"
     CDS             2990..3727
                     /locus_tag="IWL30_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAP9706675.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3882..4850)
                     /locus_tag="IWL30_001923"
     CDS             complement(3882..4850)
                     /locus_tag="IWL30_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAP9706676.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5082..5915
                     /locus_tag="IWL30_001924"
     CDS             5082..5915
                     /locus_tag="IWL30_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAP9706677.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5949..6788
                     /locus_tag="IWL30_001925"
     CDS             5949..6788
                     /locus_tag="IWL30_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9706678.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6814..7374
                     /locus_tag="IWL30_001926"
     CDS             6814..7374
                     /locus_tag="IWL30_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9706679.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7573..9294
                     /locus_tag="IWL30_001927"
     CDS             7573..9294
                     /locus_tag="IWL30_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAP9706680.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9459..10601
                     /locus_tag="IWL30_001928"
     CDS             9459..10601
                     /locus_tag="IWL30_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP9706681.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10617..11828
                     /gene="thiI"
                     /locus_tag="IWL30_001929"
     CDS             10617..11828
                     /gene="thiI"
                     /locus_tag="IWL30_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAP9706682.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            12401..13048
                     /locus_tag="IWL30_001930"
     CDS             12401..13048
                     /locus_tag="IWL30_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP9706683.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            13441..16086
                     /locus_tag="IWL30_001931"
     CDS             13441..16086
                     /locus_tag="IWL30_001931"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAP9706684.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            16396..17709
                     /locus_tag="IWL30_001932"
     CDS             16396..17709
                     /locus_tag="IWL30_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAP9706685.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            17699..18352
                     /locus_tag="IWL30_001933"
     CDS             17699..18352
                     /locus_tag="IWL30_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9706686.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            18407..19078
                     /gene="radC"
                     /locus_tag="IWL30_001934"
     CDS             18407..19078
                     /gene="radC"
                     /locus_tag="IWL30_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HAP9706687.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGIYKLVHINYIRKFTVILPSGNTIMLSIIERHNAFFSS
                     SEDSL"
     gene            complement(18973..20325)
                     /locus_tag="IWL30_001935"
     CDS             complement(18973..20325)
                     /locus_tag="IWL30_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9706688.1"
                     /translation="MKRVALYMRVSTEQQAKHGDSLREQKETLYEYIEQHKDLKVVNE
                     YVDGGISGQKINRDEFQKLLQDVKENKIDLILFTKLDRWFRNLRHYLNTQEILEKHNV
                     SWNAVSQQYYDTTTAYGRTFIAQVMSFAELEAQIDSERIKAVMANKIAQGEVVSGKTP
                     LGYSIENKKLVINDDAPIVIDIFNYFLSSGSLRKTVYYLGSQYGIVRDYQSVKNMLTN
                     KKYIGELRNNKNYCPPIIDKKLFYAVQKALPKNLKTNAKRDYIFKGLLKCSDCQGSVA
                     GQTIKARYKKKDGTESIYERTCYRCVKRRNNKLRCTNKRAFYEKNLERYIFEATKQKF
                     EQIQINYSKKQPKIIKKKNSKKNIENKLDRLKKAYLNEVIDLEEYKKDREALMKELNE
                     IEVEPAKIDIKNVEFILSKEFDEIYKESSEEEKNALWRSIIDNIIVFPDGNITVNFLI
                     "
     gene            complement(20391..>21195)
                     /locus_tag="IWL30_001936"
     CDS             complement(20391..>21195)
                     /locus_tag="IWL30_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAP9706689.1"
                     /translation="LLNKLTISNVDSTREKIIRTFDKINKLFKNDEAEFKKELLELKL
                     QELELNYSYIVKVNDEKEQQKAIKEQMIEEEKVRREIEREKKRIDKEERQFNSEITKL
                     MAYMQKASADVEKELYADKIKELEEKLRELETVKEDVLQRELNTRAGYVYVISNIGSF
                     GEDVYKIGMTRRLEPMDRIKELSSASVPFEFDVHAMIFSEDAPTLENQLHSHFRKNEV
                     NKVNQRKEFFKVSLDEIEKVVLENYNGTVSFTKLAQAEQYRRSLELTDV"
BASE COUNT         7007 a         3576 c         4283 g         6329 t
ORIGIN      
        1 tcctactgcc ggagtaaata aagcttatgc aaagaaaaag aaatccaatc acttgggttt
       61 ctttttcttt tattattatg atgaatcaag ctttctgttt ttgtcacgta tgttgtaatc
      121 tttacgaaag agtagagttg gctactcata atcaaccata cttcccatca tcgctaaata
      181 cgaaataaat cagcattcct acaccaatca aagcacaagc tgcaccaaga aaagggatga
      241 cattcccaaa gttatctgta ttcataagta tgccaccacc cacaaacaaa aagccgccag
      301 caatcaggtt tttcatttta ttcatctcct ttcttacgtt atagtatttc cttctttcaa
      361 tgagtatttt gaaagctatc ttattgtatg atgttcttct aggcctcttc tttattatac
      421 aggccatttt cgttatctca tagaaatatt cctttttatc tgaaaactat tgtataccgt
      481 atgacatttt tcttcatgaa gttgtaagta tattctcatt caaatgaaaa tttcatcata
      541 gaagagattg tatttctaaa ttcttaagag taaaatacga agtataactt ggatttggaa
      601 ggatgattgg atgaaagcta ttacgcctga tatgacaaca aaatttaaca aacagtttca
      661 agcaaatatc aaacagcagg cattgcagcg ggcagttgtg aagaacggaa ttactgcttc
      721 tgctgaaaat gtttcagcac atgtggagaa tacaccggta ttttctatag atttggctac
      781 ggggaaagtg gctaatcaaa tgcaatcggg gcgttgctgg atgtttgctg cactgaacac
      841 attccgtcat aagatgctga atcaattcca tttaaaagag tttgaattat cacaaaatta
      901 cacttttttc tgggataaat atgaaaaggc aaattatttc tatgaaaaca tcattgcaac
      961 aggaaaagag gcaatcgata gccggaaagt agctttctta ctcgctactc ctcaacaaga
     1021 tggcggccaa tgggatatga tcgtttcatt attccaaaaa tatggcgttg tgcctaaatc
     1081 ggtgatgccg gaaagtgcga atagttcaaa ttctcgagat ttgaataact acttgaacaa
     1141 attactgcgt aaagatgcag tagtgttacg gaaaatggtt gctcaaggag aaacattaga
     1201 cgctattgaa gaaaagaaag aagaaatgct agaaagcatt tataacttct tagctatctc
     1261 attaggaaca cctccaaaag aattcgattt tgagtaccgc gatgaagaaa agaactatca
     1321 tcttgaccga ggactgacac cacaaatttt ttatgataaa tacatcggtg taaaattaga
     1381 tgattatgtt agcgtgatca atgcaccaac aaaagacaaa ccattcaatc gttcttatac
     1441 agtagagatg ctaggaaatg tcgttggcgg gaaagaagtc aaatatctga atgttgatat
     1501 ggaaactttc aaaaaattag cgattgctca gcttgaagaa ggagaatctg tttggtttgg
     1561 ttgtgatgta ggtcaatctt ctactagaac tagtggcatc atggccttag atgcttattc
     1621 aatggatgat ctatttgata cggattttac aatgactaaa gctgaacgtt tagattttgg
     1681 agaaagtctg atgacccatg cgatggtatt gaccggtgtt gacttagtag acgggcaatc
     1741 aacaaaatgg aaagtcgaaa atagctgggg cgaaaaagtt ggaaagaatg gcttctttgt
     1801 tatgagtgat gcctggatgg acgaatatac gtaccaaatc gttgtaagaa aagatttatt
     1861 gacgaaagag caattaaatg cgttcaatga agaaccaatt gttctatctc cgtgggatcc
     1921 aatgggtgct ttagcataga cgagtatttc aatgaagagt atgtagtaaa gataaaaaga
     1981 gctggcttaa gccagctctt tttatcttta aagtccattc cctgtttttt atggtccctg
     2041 tgctataatt ctaaataaag gataaggatt ggaaataatc cttttatcat taaaaatatg
     2101 atgacgaagt agaataaaat tgggagtagg ggggaaagtc atggatatcc aaactgtttt
     2161 atctgatctg aaattaaatg gtaaagtgat tccagttgtt ggtggcgatg ttaatcagac
     2221 ctatcgtata aaaaccgaac atcgtgctta ttttttaaaa atccatccga atgtaaaaaa
     2281 aggatttttt gaagcagaag tagacggatt aaaagaactg tcagcattcg tacgtgtacc
     2341 ggatacgtat atgctaggtg aaacaagtga aggcgcttat ttgttaatgg aatggatcga
     2401 gccgggaaaa ggggatcaac gagatcttgc tgcagcttta gctaatctcc atcagcaaac
     2461 agcgccacaa tttggttttc gtaaagataa ctatttgggt acacttgttc agaaaaattc
     2521 ttttgaagaa gattggtgga cttttttctt taaagatcgg cttgaatccc agatttcttt
     2581 ggcagaagaa acgaatcgat ggaatgtaca gcgtcaagaa aagtatctgc gatttaaaga
     2641 acgtgtattg aaatcggttg aaccaaaaaa gatcactcca cgcctcttgc acggagacct
     2701 gtggagtggt aatgtgttct ttgaccagca agggcatcca gtttttgtcg acccagctgt
     2761 ttcttatggg aatcgggaac aagatattgc aatgagccag ttattcggtg gtttccgacc
     2821 ggaattttta gatgcttatc aaactatatt tccattagaa aagggatgga aagaccgact
     2881 gccgatttac cagttatatt atcttttggc acatttgaat atgtttggag aaagttacgg
     2941 ttcccaagta gatcaattac tagaaaattt ttaagaggag ggcgtgtttt tggcaaaaaa
     3001 atataccaaa aagcatgaag tttcatatta tgagtgtgac atcaaccaaa cgatgacatt
     3061 tccttcgatg ttaggagtag ttatcaaaac gtctgaagat caaagcgacc agttgggaag
     3121 aggcagtgaa ttcgtcaata gctttggact tacttgggtc atcacgaatt attccatgga
     3181 gattaatcgt ctccctagag tcggtgaagt aatctcagta acgactcaag ccatggagta
     3241 caataagttt ttctgttatc ggaacttctg gatccatgat gaagatggga aagaattagt
     3301 caaaatagaa tctgttttcg tattgatgga ttttgtgaat cgaaaaatga gcagtgttaa
     3361 tgaagagatc attgctcctt ttgagagtga gaagatcaag aaaatcaagc gtcaggaaaa
     3421 aattgaaaag attgattcgg gcttcatgtt gccatatcgt gttcgttttt atgatatcga
     3481 tagtaatcag catgttaata atgcgatgta ttttaattgg atcattgacg tattaggata
     3541 tgactttttg actacgcatg ttccagaaaa agtaattatt cgatttgata aagaagtaga
     3601 gtacgggaat gagatcgaaa gccattttga acaaatccct atcgaaaatg gtgtcaaaac
     3661 aaaacatgaa atccgaatgc aagaacagct gtattgtgaa gcaaatatta tatggaaaaa
     3721 taaataaaaa agaggttgtg acagaagggg gcaacttcaa gaaataagaa gaaatttacg
     3781 aaaattgttt ctcaaatttt tgtaaatttt cgacttagtt ttcgaagaag ctatttttgt
     3841 cacagcctct ctttttaagt ttagctatta gataatgatg tttagttttg gataaacgaa
     3901 atcaattctt cacccgacaa accatagttt acagcaatca atagttttaa acgtgctttt
     3961 tgactattaa tactgttaca gaaaataatc cccatttttt ctagttcttt tcccccgcct
     4021 tcataatcat aaacaggctc tgcgattcca ttgaaacaac gagaaacaag tactacaggg
     4081 attttttgga caagaagctt ttccaaagca gctaaggctt gtggagggag attccccgca
     4141 cctaaagctt cgattaccaa accgtctact ttcgtatggg cgatggcttc caataagtcc
     4201 ccttgcatcc cagcatatgc tttaacgatc gggatagtcc catctacagc ggaaatgtcc
     4261 agtcgctttg tttctaaaag ttcctgcaag aataaaattc gattcttagt tactaagccg
     4321 atgggaccca atgtaggtgt tcggaaagta gcaacatttg tggtatgggt ctttgtcacg
     4381 tagcgtgcag aatggatctc atcgttcatg accaccaaaa cccccttatc tagcgcttct
     4441 tcacaacttg cgacacgaat cgcactttca aaattataaa ggccgtcact tccaagttca
     4501 ttacttgaac gcattgcccc agtcaaaaca attggtaatt tgttgccaat cgttgtatca
     4561 agaaaaaaag cagtttcttc taatgtatct gtaccgtgtg tgatcaccac tccggatata
     4621 cctgatgcat aagcggacaa gatacgttct tttaatacca acatgtgaga aggcatgatg
     4681 tgtggtgagg gaagattgaa gatatcttca actaataatt ctacatgctt ggggattgtg
     4741 atttctgcat ctaataatgg gtttgccaca tcaggactaa caccgcccgt gagcatgttt
     4801 tctttcatgg cgatcgttcc gccggtgtgt agtacaagta tttttttcat tttacagact
     4861 tccttacgaa taattttgca tagccgttaa atcttattgt atactatata aaagagtttt
     4921 gctatcagtg aagaataacg aatagcctaa cttatcataa aaaagctgac cttattaaaa
     4981 tggaaaagac tcaaccttga cctcctaata caatggagta tctggttgag tttcttttac
     5041 aagaaagcag ccaaggaaat caatcacgaa ggtggccgga aatgatcaga atcggattaa
     5101 catcgtttaa agaacatgac aaattaactg gtaaaaagaa taacactctt tatgaatatg
     5161 cagggtttct acccttagtg gaattagata ctgcctatta tggaattccc cgaaagtcct
     5221 cagttgagaa ttggataagt gaagtacctg aatcatttcg ctttgtagtc aaagtctatc
     5281 aaggaatcag cggtcaagga gactggaagc cttattatca gaccgaagaa gagatgattg
     5341 cgcatttttt agatgttatg tcgccaatgg tagatgctgg aaagatgttt tgctttttag
     5401 tccaattccc ctcatcattc aaatgtacga aagaaaatgt ccagtacttg cgcaaactta
     5461 gaaagtggtt tgagggttat cctgtttcta tagaattgcg ggattattct tggtacagcg
     5521 atgaatttct tgaacgtaca agaaacttta tgtctagagc cgctttttct ttagccatca
     5581 tcgatgagcc acagttgctg aatacaacga ttccttttga tccatttgtc accaatccga
     5641 actttgctct ttttcgattc catggtagga atgcgcaagg atggcaagca aatgataaag
     5701 agtggcgtaa aaaaagaacg ttatatcgtt attcagaaaa agaactgcaa gaactagcac
     5761 atgaagtcaa agtagttgat tctaagacaa aagaggtagg tgtgattttt aataataatt
     5821 ctggtggtga tgcggctgaa aatgcattag cactgcaaaa aatattaaat atcaagccag
     5881 atggactcaa tccaacccaa atggatctgt tttaagggct tattttattc aagaaagggg
     5941 cgaagtctat gatcaaagct attttttttg atattgacgg aacattggtc accaatcgtt
     6001 caaaagcgtt ggaatcaaca aaacaagcaa ttgaatatgc tcggaaaaac ggcattttat
     6061 gcggtgtagc aacggggcgc agcccagtaa agatcaaaga aatcattgat gaattggaat
     6121 tagatatgta tgtcgtgtat aatggtcaac tggtttttac tgcagatcgc acgattatcg
     6181 accacccttt tgaacaaaaa gtattggaac atattgttga atttgcagat gagaaccatc
     6241 gacaaatcgt ttttggtgcg agaaatcggt tagatggatc aacgacgatg ctattaggac
     6301 aatcgatttt tatcaagcga ctagtcagct ttttaccgcg taaattcccg gtacgtctga
     6361 tgaaaaaaat cctccaggtt tttagccctc accgacaaaa ggatcgttat gaaacgttag
     6421 agatattaaa agaaccaatt tatcaatgta tattgctcag tccggagtca gaacagaaaa
     6481 agttagaaca tcgctttccc gagtgtacgt ttcagcgttc aaatacgtat actgtagata
     6541 tcatcccaaa aggtggttca aaattactag gcatccaagc tttcgcaaat gctgtcgaaa
     6601 ttgaaatgga agaaattatg gcattcggcg atcattacaa tgatatcgag atgctgaaag
     6661 gtgtaggaat tggagtggcc atggggaatg ctcagatcga agtaaaacat gctgcagact
     6721 ttgtgactca atcaaatgaa gaagatggga tatacgaagg actgaaacac ttcggtctga
     6781 tttattagtc aatgaaagaa ggaaaaaatc agtatggaag cagagacacc ttttcaaaaa
     6841 gtagaaacat tccacaaaac ttttgatcca agaagaccag agatacccac ggcttttaca
     6901 gaaaatgaag ctcatacacg tgctgcgttc aaaatagaag aacttgttga atttgttgca
     6961 gctacttcac ctaataaaga acaactgcat gaagctgtta tgctgctgca cgaagcatta
     7021 gataaagcag aagaaaaaat acttggtaaa acacagtggg gaagtgatcc gctggttgaa
     7081 caagtagatg cactgacaga tatcctttac tttacttatg gttctttttc attactagga
     7141 atagtgcctg atcgaatatt cgaaatcgtc catcaggcaa atatggggaa attatttcca
     7201 gatggaaaac ctcattatga ccctgtaaca aataaagtgt tgaaacctga aaactggcag
     7261 atggaatatg ctccagaacc taaaatcgcg gcagaaatcc aacgtcagat tcaagaagcg
     7321 attgctaaga agaaatccgc tgatataggc atgtgcacaa atgaaaaaga gtgatcagag
     7381 tttcactatt tttgttatct ttatacgatg gaaaaacaaa agattttcgt ttaagttagt
     7441 gaatgcatga aaatagtatg ctataatcta gaatagatgt cttttgcacg agagacgtat
     7501 tatttggatt tatgccaagc agaacgccat ctgttgcata aaagatgcat actgaaaacg
     7561 gaggaaccat aaatgggaaa taatgttatc atcggaatcg tcattgcaat cattatagtc
     7621 gccattgtcg tctttttggt tagttacttg atgaagaaaa aaaaccaaga tcgattggat
     7681 gccttagaaa aacgcaagga agaattattt gacttgccag tgattgaaga agtcgatgat
     7741 gttaaaaaaa tgcatctggt tgggcaaagc caaaatacct ttcgtgaatg gagtcaaaaa
     7801 tggaatgaca tatctacttc ctcgttcgct gaactagaaa gtcaaatttt cgaagtggaa
     7861 aacctcaatg aaagttttcg gttcttaaaa ggaaaaaaag cagttgttga agcagaagct
     7921 acaatggacc aaatggagaa acaagtgacg gaaatccgtg aaggtctaaa ggaattgcgg
     7981 gaaacggaag aacgcaactc actagctgtt cagcaggcac ttgacgtcta tgaagaactg
     8041 aaagaaaaag tcagagaaga cgagcatgaa tacggaccag ctctttttga attgcaaaaa
     8101 cagatcaaag tgatcgaaaa agaatttacg cagtttgtga ctttaaacac ttctggagac
     8161 cctgttgaag cccgcgaggt tttggataaa gcagaaaaac atacgtatga agtggaagct
     8221 ctgatgaaaa aaattccacc gctgtatgag gagctccata ccactttccc tgaacaactt
     8281 gaagaaatca gtgatactta cgagaaattg atggaagaac aatatgtatt cccagaagaa
     8341 gatcttgctg aagatatcgc aaaagtaagt cgaagaattg aaaattcatt agccaatctt
     8401 gaaaaaacag aagttgaaac ggtcgaattt gaaaaccgtg aaacagctga tttgattgat
     8461 tcgttatatg atatcttaga acgggaaatg gaagctcaga gatatgtaaa aactaaccaa
     8521 tcaacgattg cagagtatat caaacataca acaaagaata accgtcaatt attgattgag
     8581 ctggatcaca catctcaaag ctatacattg aaccacaatg aaattggccg tgtacgaggt
     8641 ttccaaaccg aagtggaaga aatggaacgt caaaatgaac agatgattcc acagatccgt
     8701 caacacgaaa ttccttattc agaagtgcgc acgttctata aaacagtctt caaagtctta
     8761 gaagatatcg aaaagcaaca agtagagatc gacgactcat tacatgaatt gagaaaaggc
     8821 gaaaaagaag cgcaagaaaa gatcgataca ttcgaattca aattgcgcag tttgaaacgt
     8881 tttgtagaaa aacaacgttt gcctggcttg ccgaatgatt acctagaatt cttttttgtt
     8941 gctactgacc gtattgaaga actaagtgtt gtcttgaata aaattcgagt aaacatggaa
     9001 gaggtcaatc gtttagttgc tctatgtgaa gaagatctag aattattaga taagaagaca
     9061 catgatttag tagatgcagc agcattaacg gagcaaatgc tccaatatgc aaatcgttat
     9121 cgtcatacac acgaagaagt gagagaagca attgataaaa gctactattt attcaataaa
     9181 gaatatcact atcaagaagc attagacgaa atcggtacag cattagagcg agtagaacct
     9241 ggtgctttca aacgcatcga agatttttat tttaatcatc cagacttagt ataaagcaaa
     9301 aaagttagta aaccagcaag agcaagacgg attattgtct tgttcttgtc tttttttgta
     9361 tatttgaaaa ttgaaacaag tgaaaatttc acctgctctt gaaagcacct tggacaaccg
     9421 ttataataga aaagaatttc aaaaaagagg ggtttcttat gatatatttt gataacagtg
     9481 caacaacccc aatctttccg caaagcttag atgcatatgt aaaaaccagt cagcggatta
     9541 ttgggaatcc atcaagcttg catgatttag gcaatcaagc taaccggttg ttgcagcaag
     9601 caagaaagca gatcgctgat ttgatccatg tagaaccaga agaaatctat tttacttcag
     9661 gcggaacaga aggagataac tgggtattaa aaggaacgat gatcgaaaaa agagaatatg
     9721 ggaatcatat gatcatttca ggagtcgaac atccggccgt cagcgagaca gcagaacaat
     9781 taaaagaatt aggaatcgaa gtaagtatcg ccccagtgga taaacgcgga tttgtacgtg
     9841 tcgacgaatt gaaagaatta ataagaaaag agacagtatt agtctctgtt atggctgtga
     9901 ataatgaagt aggatcgatt cagcccatcc aggaaatcag tgatcttttg gatgattttc
     9961 ctaaaattca tttccatgta gatgctgttc aggcgattgg aaaagtacca ttctcgcaat
    10021 ggttgacaga aagagttgat tttgcaacat tttcagctca taaattccat ggcccacggg
    10081 gaactggttt catttattgg aaaaagggtc gtcgtttagc accattactg acaggtggag
    10141 ggcaagaaag aaatcagcga agtgggacag aaaatgttcc aggtattgtg gctatggtta
    10201 aagctttacg tttatcatta gagataaagg aacaaaaacc tgagcagaca caacgcttaa
    10261 aagtagctct aatcaatgcg ttgaagacgt atgagaaagt gacgattttt tcagaaggaa
    10321 ctgactatgc accgcatatt ttatgttttg ctctaaaagg aattcgcggc gaagttctcg
    10381 tccatgcctt agaagaaaaa caaatatttg tttctactac tagtgcctgc tcctcacgta
    10441 aacatatggc aagcagtaca ttgcacgcaa tgcatgtacc aaatgatctg gctacatctg
    10501 ctgttcgaat cagcttagat gaaaacaatt cgcttgcaga agtcgagcaa tttatgatta
    10561 ttttcaagca gctttataag aaattttctg caatcaatta aaagaggagt gttcttgtga
    10621 aatacagtga aattatggtc cgttatgggg aactttcgac aaaaggaaag aatcgaaaaa
    10681 gctttatcat gcaactagca caaaatgtca gacaagcttt gaaagacttt ccagaaatca
    10741 aaatccatgc tgatcgggac cggatgcatc ttttattgaa tggcgcagac gggcagctag
    10801 ttattccaaa actagcaaaa atatttggga tccaaaattt ctctccaagt attcgtgtag
    10861 aaaaagatgt cgaagttttg aaacaagctg ttcaagacat catgaaagaa atttatactg
    10921 ggaaagaaac atttaaaatt atcgctaaaa gaagcgatca ccaatttgaa ttgacaagta
    10981 atgaattgaa tcaaacatta ggtaatgctg tttttgatat tttcccgcat atccaagttc
    11041 aaatgcgtca accagatatt ccattacgtg tagagattcg gcgtgatggc gcttatctat
    11101 cgtatgaaac gatcaaggga gctggaggat tgcccgtagg aacaagcgga aaaggaatgc
    11161 ttatgctttc tggggggatt gattctcctg tagcagggta ctttgccatg aagcggggag
    11221 tggaaattga agctgttcat tttgctagtc cgccgtatac aagtgaacaa gcactgcaaa
    11281 aagcaaaaga tctaacagcc aaactagcac cttatgtggg aacgatccat tttatcgaag
    11341 tgccatttac cgagattcaa gaagaaatca aaaagtcagt gccacaagga tattggatga
    11401 caattactcg tcgaatgatg ttgcgtttga ccgatgaaat tcgccgtatt cgtcatggtt
    11461 tagtcatttt gaatggtgaa tcattaggtc aagtggcttc acaaactttg caaagtatgg
    11521 tggctatcaa tgaagtaacg aatacgccaa tcattcggcc tgtagcgaca atggataaac
    11581 ttgaaatcat tgaagtagca gaacagatcg atacatttaa tctttctatc cagccttttg
    11641 aagattgctg tacgattttt gctcctccac agccaaaaac acgccctcaa ctggaaaagg
    11701 tccatcaata tgaagagcgt ttagcaatag atgaaatgat ccaacgagca ctagctggct
    11761 tgaagatgga agagatacaa gcagaatcgt ctaatgaaaa aactgaagaa ttttcagaat
    11821 tgctataaga ggttatgaaa atagtgtttt gaccagagta ttagagaaac aaaacgaaaa
    11881 aatagctcca tgtattttac cgtttttgaa ctaatgtcga agattagctg attgaatacc
    11941 atttatcaag aagttatgga aaagccggtt cttttttact aactgaaaaa taaaatttta
    12001 tttagagatc aagctacagt tgataaatga tataaaaaag agaatttccc atgaaatagc
    12061 gaggttgtga cagaagtggg cagcttcaag aaataagaag gaatttacga aaattgcttt
    12121 tcaaattttt gtgaaatttc ggcttatttt tcgaagaagt tacttctgga acaccgtgta
    12181 tcagaggttg cgaaaaagct gccctgcatc aagtaataag aacagccaaa caaaaatagt
    12241 tcctcatatt ttacgtttgg ttgagcttat taccgcagat gcaagctttt gaacaccgtt
    12301 ttatttgtct cacacccttt tccttcctac ttgtaacctt gctcccgtgt tagaaacatg
    12361 ttacactaaa tatgtgaaat tattaacaag gagcgtcgtt atgcgagaaa aaacagatac
    12421 actgccaaaa gcaactgcga aaagattacc tttatattta cgctacttga agatgctgga
    12481 tgactcaggt ataagtagaa tcaaatcaaa tgagttcagc gaaatcaccc aaattccttc
    12541 tgcaacgatc cgcagagact tctcgcaatt aggcgagcta ggcagaagcg gatatggcta
    12601 tgatgtccct tttcttatcg atgtattcaa taatatcctt aatactaaag aagaaaaacg
    12661 aatcgcatta gtaggatatg gaaatcttgg caaagcattg aaacacaaca attttagaag
    12721 aaatgaaaat ctaaatatcg tgtgcgtctt tgataatgat ccagcactga tcaatcgagt
    12781 aattgatgga gaaatgatct atccgattga tcgtttcgcg gagatagcca aagcaaaaaa
    12841 tgtgacagta gcaatctcta cggtaccaag taaatattca caatctgcta ttgatgaaat
    12901 tgtcaaagga aatgtaaccg ctatacttaa ttttgcgcct gacagagtaa ctgtcccagc
    12961 ttatgtcaat gttcaataca ttgatttgac aaccgagttg cagacgctta tttactttga
    13021 tgagaattac tcagaagttt tttcttagga aaatttgagt aggcttattg atcttgcgag
    13081 aactcgtcgt aattctttca aaaatttttt agtactaaat gaagtggttt tttcacgacc
    13141 acttgttgac taaaaaaaaa aaccattgta gaatgaagca taacgctgaa caagaggagt
    13201 aactggcaca ttcattctta gagagagaac tatctgctga aaggttctca atgacaacca
    13261 gtgaaggtag cttgggagtt tctgtcttga atggagtaga gatagacgag tcgtgatcgt
    13321 taactcactt gagaggcaat aatgattgat tattgcaatt taggtggtac cgcgtaaatc
    13381 atacgtccta gaatacgaaa gtattttagg acgtttttta gtttaaagga ggaaaacagc
    13441 atgacggaag aaaataacct atcaacaaaa tataatccgc aagaagttga agctggacgt
    13501 tatcagcatt ggttagaaca agatttgttt aaaccgagcg gagataaaaa agcaaaacct
    13561 tattcaatcg taatcccgcc cccaaatgtt actggaaaac ttcatttagg gcatgcttgg
    13621 gatacgacct tgcaagatat gctgattcgt caaaagagaa tgcaaggata tgatacttta
    13681 tggctgcctg gtatggacca tgcaggaatc gcgacacaag caaaagtaga agcgaagctt
    13741 agagagcaag ggatttctcg ttatgatcta ggtcgtgaaa agttcatcga tcaagtgtgg
    13801 gaatggaaag aagagtatgc gagccacatc cgcgaacagt gggctaaaat gggcttatct
    13861 ttagattaca gtagggaacg ttttacatta gatgatggat tatctgaagc agtacgtaaa
    13921 gtttttgtta ccttatacga aaaaggtttg atttatcgtg gtgaatatat catcaactgg
    13981 gatcctcaag cacgaacagc tctatctgat atcgaagtta ttcataaaga tatcgaaggc
    14041 gccttttatc atatgagcta tgaactagca gatggatcag gcgtagtaga aattgcgaca
    14101 acccgtccag aaacgatgtt gggagatacg gcgattgctg ttcatccaga agacgagcgt
    14161 tatcaagcgc tcatcggcaa aaaagtgatt ctacctttag tcaataaaca aatcccaatc
    14221 attgcagatg aatatgtaga tatggaattc gggacaggtg tagtaaaaat cactccagca
    14281 catgatccaa acgactttga ggtaggtaat cgtcatgatc ttccacgcgt aaatgttatg
    14341 gatgatgatg gtacgatgaa tgaactagca ggaaaatatg ctggaatgga acgttttgct
    14401 gctcgtaaag caatcgtgca agatctgaaa gaaatcggtc gattgattaa aatcgatacg
    14461 atgatccaca gtgtgggtca ttccgagcgg acaggtgtcg ttgttgaacc tcggctgtct
    14521 actcaatggt ttgtaaaaat ggcgccgtta gcgaaaaaag ccattgataa ccaagaaaca
    14581 gatcaagcag tggaattctt ccctccaaga ttcaatcaaa cgtttttacg ttggatggaa
    14641 aatgtccatg attgggtaat ctcacgacaa ttatggtggg gacatcaaat tcctgcatgg
    14701 tatcacaaag aaacaggtga aatgtatgta gggatggaag caccagcgga tagtgaaaac
    14761 tgggtacaag atcctgatgt tttagacact tggtttagtt cagctttgtg gcctttctca
    14821 acaatgggct ggccagatac ggaaagcgaa gattataagc gatacttccc tactagcact
    14881 ttagtaaccg gttatgacat cattttcttc tgggtaagca gaatgatttt ccaaagcttg
    14941 gaatttactg gcgaacgtcc atttgagaat gtcttgattc atggattgat tcgtgacgaa
    15001 caagggcgga agatgagtaa atcattaggt aatggaatcg atccgatgga agtaatcgaa
    15061 aagtatggag cagatgctct ccgctggttc ttatcgaatg gttctgcacc aggacaagat
    15121 gtccgcttca gctatgaaaa aatggatgct tcttggaact ttatcaacaa aatctggaat
    15181 gcttcacgtt tcgttatcat gaatatcgaa ggaatgaccg tagaagagat cgatttatct
    15241 ggagaaaaat cagtggctga ccggtggatc cttactcgtt tgaatgaaac gatcgaacga
    15301 gtaactgagc tatttgatcg ttttgaattt ggtgaagcag gtcgacaact gtataacttt
    15361 atctgggatg atttttgtga ctggtacatc gaaatgagta aagaagtact ttatggagat
    15421 gatgctgttt caaaacaaac cacccgtagc atcttagtcc atacacttga tcagatccta
    15481 cgtttattgc atccaatcat gccattcgtt acagaagaaa tatgggaaca tattcctcat
    15541 cagggaaatt ctctagtagt ggcagaatat cctgttgtac gtccagagtt tgatgatgaa
    15601 acagcttcta aagggatgga agtattgaaa gaattgattc gagccgttcg aaatattcgt
    15661 tcagaagtga atacaccatt gtctaaacca attacgttgc taattaaaac aaacgatgct
    15721 aaaatcgatc aatttttagt tgaaaataca agctatatcg aacgcttctg taatcccgaa
    15781 gagttgacga tatcaagtga gatcactgcg ccagatctag cgatgtcagc tgttctgaca
    15841 ggtgcagaaa tcttcttgcc gttagcagga ttgatcaata ttgaagaaga aatcaaacga
    15901 ttagaaaaag aactggaaaa gtggacaagc gaagtaaaac gtgtccaagg aaaactttct
    15961 aatgaacgtt ttgtttctaa cgcgccggaa gaagtagtag aggcagagcg tgcaaaagaa
    16021 aaagattatt tagagaagca aacggctgtc aaagaacgaa tcgaaagttt gcgtactatt
    16081 caataagaat agaaaaagag agtgtgaaat cagctagcta ttactaaaag ctagctgatt
    16141 gaacatcgtt acaggaaaga gtttgagcct cactcttagc ttcgattcat aaggagatgt
    16201 gacataaatt ttgtcgcatc tcttttttag tctactctta ttatgtagga gaaggaggga
    16261 ggtggatcat gtatcaagag tcaataaagt agttgataaa atcgttcctc tctctgtttc
    16321 ttttctcttt gtcatacttt tatttttctt atgtgttcta tttgttataa taaaagagaa
    16381 atgggggaat tgttgatgac gattgaagaa gcaatagaat ggatccatag tcgattacca
    16441 tttggttcac gtcctggcct tgaccgagtc gaagctttat tagaagtggt agggcatcca
    16501 gagagaaaag taaaaacgat ccatatcgct ggaacaaacg gaaaaggttc tactgtagct
    16561 tatttgagaa gcctattaga agaggctggg ctgacagtag gtacatttac ttcaccttat
    16621 atcgaaacgt tcaatgaacg gatcgcaatc aatggcaaaa gcattccaga caatgatttg
    16681 atccagcttg taaaaaaata tcagccactg atcaaacaac ttgatcaatt tgaagcagta
    16741 gctggtatta ctgaattcga gacattaaca gcaatggcat ttgattattt tttgatgaag
    16801 aaagtggacg tagcgattat cgaagttggc ttaggcggct tattggattc taccaatgta
    16861 gctgtaccaa tgctgacggg aataacaacg attggacttg atcataccga aatacttgga
    16921 gatacaattg aagaaatcgc tgcacaaaaa gctggaatca tcaaacaagg cgttccagta
    16981 gtaacaggaa atattgcccc agaagctttt actgtcatcc aacaaacagc taaagcaaaa
    17041 aatgcaccgc tttatgtata cggggaaaat tataaaataa actatctgca tcctgatgag
    17101 atgtggggag aagtatttga attttttaat gaaataggaa agatccctca attaaccact
    17161 ccgcttttag ggcaacatca agctgaaaat gcaggagtag ccatcgaatt gtattttgtt
    17221 tactgtcaat tggaaaagct gcctttccga gaaaaagatg ttttgaaggg attgaagaaa
    17281 tctcgatggc caggaagaat ggagcgtttg agtaacgagc cattagttgt tttagatggt
    17341 gcccataata cgcatgcaat gaaccggctt gttgagaatt tgaaaaaaga attcaaagga
    17401 tatcaaatca acattttgtt ttccgcctta cgaactaaaa atgttagaga aatgctagaa
    17461 cgattgcagc aagtaccaaa tgtccatatt tatttgacga cctttgaaca tccaaaagcc
    17521 atcgaattaa gtgaagtatc agatttagca agtgagagag tatcaatcgt ttcattgtgg
    17581 caattcggat tagcagatat attggaaaag atgacaaatg aagacgtatt agttgtgacc
    17641 ggttcacttt atttcatttc agaagttcgc catttattaa tacaattagg aggagatcat
    17701 gaagtataat gcagtgattt ttgacatgga tggtttgctg ttcgacacag agttagtgta
    17761 ttacgaagcc acacaagtag tagcagacaa gatgggtttt ccttatgata aagacctgta
    17821 tttaaaattc ttaggtgttt cagatgaaga agtatgggcg aattatcata tgattttctc
    17881 ggaatttggc aaaacaagtg tacaaagatt catagatgat tcataccagg aaacgcttga
    17941 acgtttttcg caaggagcgg tacaaatgaa gcccggtgtg ttggaactcc tctctttttt
    18001 agaagaacat cagattccca aagttgtcgc ttccagtaac caacgagcag tcattgaact
    18061 tttattgaag aagaacaatc tgtttgaacg atttgagacg attgtttctg cagaaaatgt
    18121 tcaacgagca aaaccagacc cagaaatttt cttgttagct aatcagaagc taggtacagc
    18181 gaagaaagaa acattgattt tagaagattc acaaaatggt attttagcag cttctgcggc
    18241 tgagatcccg gtagtcatgg taccagatct gcttccacca tcccaagaat tgatcaaaaa
    18301 aacagctggg gttgtttctt ctttacatga aatccctgca ttgttaaaat aaaaatccat
    18361 ctcttttcgt atcttatttt agataagata gaaaggagat gtttttttgg gaaaacgatt
    18421 gatcaaagaa gtacctacta gctctttacc aagggaacgt atggaaattt atggcgcgga
    18481 agctttatcg gatcaggagc ttttggcaat cttactgcgt accggtcaac acccttatag
    18541 tgtgatgtcg attgctggaa atctgttaaa aacgtttgga ggactggcct ctttacgaca
    18601 ggctactttg catgagttag aagagattcg agggatagga cgcgtgaaag caatcgaaat
    18661 caaagccttg atcgaattag gtcgacgaat tcagacggat catgaaagag cggctccacc
    18721 ggttcgtacc agctacgaat tggcccagca attgattctg gaaatgaaag atcacaaaca
    18781 agaacatttg gtttgtattt atttggacac gaagaatcaa gtgatcttaa aaaaaacagt
    18841 gttcatcggt tcgctgaatc agtcagtcgc tcatcctaga gaaattttcc attatgctgt
    18901 acgttattgt gcagcaagga tcgttttagc gcataatcat ccttccggaa tatataagtt
    18961 agtacacata aattatataa ggaaatttac tgttatattg ccatctggaa atactattat
    19021 gttatcgata attgaacgcc ataatgcatt tttttcttct tccgaagatt ctttataaat
    19081 ttcatcgaat tcttttgata gaataaattc gacatttttt atatctattt tagctggctc
    19141 tacttcaatt tcatttaatt ctttcattaa agcttctcga tcttttttgt attcctctaa
    19201 gtctataact tcattcaagt aggctttctt caatctatct aatttattct caatattttt
    19261 cttactattt ttctttttaa taatctttgg ttgttttttt gaatagttta tttgtatttg
    19321 ttcaaatttt tgtttagtag cttcaaaaat ataacgttct aaattttttt cgtaaaaagc
    19381 gcgcttattt gtgcaacgta atttattatt tcttcgtttt acacatctat aacaagtcct
    19441 ttcgtatata gattctgtac cgtctttttt cttatatctt gctttaatag tttgaccagc
    19501 tactgatccc tgacagtcag aacattttaa taaaccttta aaaatgtaat cacgttttgc
    19561 atttgttttt aaatttttag gtaaggcttt ttgaacagca taaaaaagtt ttttatcaat
    19621 aataggagga cagtaatttt tattatttct taattcacca atgtattttt tgttcgtcaa
    19681 catattttta acactttgat aatctctaac gattccatac tgagagccta aataataaac
    19741 agtttttctt aaactaccac tagaaagaaa gtagttgaat atatctatca cgataggagc
    19801 atcatcattt attactaatt tcttattttc tatagagtag cctaacggag ttttcccgct
    19861 tactacttca ccttgtgcaa ttttatttgc cataacagct ttgatgcgtt ctgaatcaat
    19921 ttgggcttcc aattcagcaa aactcattac ttgtgcaatg aaagttctgc catacgctgt
    19981 agttgtatca taatattgtt gagaaacagc attccaagaa acattgtgtt tttctaaaat
    20041 ttcttgagtg tttaaataat gtcgtagatt tctaaaccat ctatctaatt ttgtaaataa
    20101 aattagatct attttatttt cttttacatc ttgtaatagt ttttgaaatt catcacgatt
    20161 tattttttga ccagaaatac caccgtctac atattcatta actactttca aatccttgtg
    20221 ttgttcgata tattcataga gggtttcttt ttgttctcta agactatctc catgtttggc
    20281 ttgttgttca gttgatactc tcatatataa tgctactcgt ttcatgttat ctcctttcga
    20341 atgtatgttc ttatcgttgt aaaagaaaag cccgaaggct aatcttttta ctacacatct
    20401 gttaattcta atgatcttct gtattgttca gcttgagcta gtttagtaaa agatacagtt
    20461 ccgttataat tttctaaaac aactttttca atttcatcaa gagatacttt aaagaattct
    20521 tttcgttgat taactttatt gacttcgttt tttctaaaat gcgaatgtag ttgattttct
    20581 aatgttggtg catcttctga aaatatcatt gcatgaacat caaattcaaa aggtacagaa
    20641 gcgctactaa gttccttaat tctatccata ggttctaacc gtctagtcat tcctatttta
    20701 tagacatctt caccgaaaga tccgatatta ctgatgacat aaacatagcc agctctagta
    20761 ttaagttctc tttgcaatac atcttctttg acagtttcta gttctcttaa tttctcttcc
    20821 agttctttaa ttttatcagc atagagttct ttttcaacat cagcactagc tttttgcatg
    20881 tatgccatta acttagttat ctctgagtta aactgtcgtt cttctttatc aatgcgtttc
    20941 ttttcacgtt ctatttcacg tcttaccttt tcttcttcaa tcatttgttc ttttattgct
    21001 ttttgctgtt ctttttcatc gttaacttta actatgtatg aatagttgag ttctaattct
    21061 tgaagtttta attcaagtaa ttcttttttg aattctgctt catcgttttt aaaaagcttg
    21121 tttattttat caaaagttct tataattttt tctctagttg aatctacgtt agaaatagta
    21181 agtttattga gaagt
//
LOCUS       DACDUU010000054        20717 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.054, whole genome shotgun sequence.
ACCESSION   DACDUU010000054 DACDUU010000000
VERSION     DACDUU010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.054"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            168..362
                     /locus_tag="IWL30_001937"
     CDS             168..362
                     /locus_tag="IWL30_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706690.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="IWL30_001938"
     CDS             631..930
                     /locus_tag="IWL30_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706691.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="IWL30_001939"
     CDS             complement(1027..1461)
                     /locus_tag="IWL30_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9706692.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="IWL30_001940"
     CDS             complement(1693..2403)
                     /locus_tag="IWL30_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP9706693.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="IWL30_001941"
     CDS             complement(2393..3253)
                     /locus_tag="IWL30_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAP9706694.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="IWL30_001942"
     CDS             complement(3258..3896)
                     /locus_tag="IWL30_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP9706695.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="IWL30_001943"
     CDS             complement(3911..4210)
                     /locus_tag="IWL30_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9706696.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="IWL30_001944"
     CDS             complement(4253..5737)
                     /locus_tag="IWL30_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP9706697.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="IWL30_001945"
     CDS             complement(5757..6218)
                     /locus_tag="IWL30_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9706698.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="IWL30_001946"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="IWL30_001946"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAP9706699.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="IWL30_001947"
     CDS             7521..8291
                     /locus_tag="IWL30_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9706700.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="IWL30_001948"
     CDS             complement(8564..9391)
                     /locus_tag="IWL30_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9706701.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9959..11194
                     /locus_tag="IWL30_001949"
     CDS             9959..11194
                     /locus_tag="IWL30_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP9706702.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12193..12453)
                     /locus_tag="IWL30_001950"
     CDS             complement(12193..12453)
                     /locus_tag="IWL30_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP9706703.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12480..13781)
                     /locus_tag="IWL30_001951"
     CDS             complement(12480..13781)
                     /locus_tag="IWL30_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9706704.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14073..14531)
                     /locus_tag="IWL30_001952"
     CDS             complement(14073..14531)
                     /locus_tag="IWL30_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP9706705.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14544..15869)
                     /locus_tag="IWL30_001953"
     CDS             complement(14544..15869)
                     /locus_tag="IWL30_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAP9706706.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15886..17298)
                     /locus_tag="IWL30_001954"
     CDS             complement(15886..17298)
                     /locus_tag="IWL30_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9706707.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(17315..18079)
                     /locus_tag="IWL30_001955"
     CDS             complement(17315..18079)
                     /locus_tag="IWL30_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAP9706708.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18183..19058)
                     /locus_tag="IWL30_001956"
     CDS             complement(18183..19058)
                     /locus_tag="IWL30_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9706709.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19313..19397)
                     /locus_tag="IWL30_001957"
     tRNA            complement(19313..19397)
                     /locus_tag="IWL30_001957"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19361..19363),aa:Leu,seq:caa)
     gene            complement(19415..19486)
                     /locus_tag="IWL30_001958"
     tRNA            complement(19415..19486)
                     /locus_tag="IWL30_001958"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19452..19454),aa:Gln,seq:ttg)
     gene            complement(19496..19568)
                     /locus_tag="IWL30_001959"
     tRNA            complement(19496..19568)
                     /locus_tag="IWL30_001959"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19533..19535),aa:His,seq:gtg)
     gene            complement(19630..19714)
                     /locus_tag="IWL30_001960"
     tRNA            complement(19630..19714)
                     /locus_tag="IWL30_001960"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19678..19680),aa:Leu,seq:caa)
     gene            complement(19735..19805)
                     /locus_tag="IWL30_001961"
     tRNA            complement(19735..19805)
                     /locus_tag="IWL30_001961"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19771..19773),aa:Cys,seq:gca)
     gene            complement(19851..19922)
                     /locus_tag="IWL30_001962"
     tRNA            complement(19851..19922)
                     /locus_tag="IWL30_001962"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19888..19890),aa:Gln,seq:ttg)
     gene            complement(19934..20006)
                     /locus_tag="IWL30_001963"
     tRNA            complement(19934..20006)
                     /locus_tag="IWL30_001963"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19971..19973),aa:His,seq:gtg)
     gene            complement(20009..20082)
                     /locus_tag="IWL30_001964"
     tRNA            complement(20009..20082)
                     /locus_tag="IWL30_001964"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20048..20050),aa:Trp,seq:cca)
     gene            complement(20089..20169)
                     /locus_tag="IWL30_001965"
     tRNA            complement(20089..20169)
                     /locus_tag="IWL30_001965"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20133..20135),aa:Tyr,seq:gta)
     gene            complement(20171..20246)
                     /locus_tag="IWL30_001966"
     tRNA            complement(20171..20246)
                     /locus_tag="IWL30_001966"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20211..20213),aa:Phe,seq:gaa)
     gene            complement(20282..20354)
                     /locus_tag="IWL30_001967"
     tRNA            complement(20282..20354)
                     /locus_tag="IWL30_001967"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20319..20321),aa:Asp,seq:gtc)
     gene            complement(20364..20436)
                     /locus_tag="IWL30_001968"
     tRNA            complement(20364..20436)
                     /locus_tag="IWL30_001968"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20401..20403),aa:Val,seq:tac)
     gene            complement(20452..20523)
                     /locus_tag="IWL30_001969"
     tRNA            complement(20452..20523)
                     /locus_tag="IWL30_001969"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20488..20490),aa:Glu,seq:ttc)
     gene            complement(20538..20627)
                     /locus_tag="IWL30_001970"
     tRNA            complement(20538..20627)
                     /locus_tag="IWL30_001970"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20591..20593),aa:Ser,seq:gga)
BASE COUNT         6547 a         4334 c         3286 g         6550 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgtccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatgcttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
     9481 aaaaatttta attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt
     9541 tctttttgtg ttatctgaat aattatcgtt tacagttata acacatagtg aaaattgtca
     9601 tggcgagaaa gtctaagaat atttttaaac ataactattt ctttcttacg tgtgtataat
     9661 gacgacagca cacctatcaa ttaatataat tattttattt ttttatgttc tcatgtttat
     9721 ttttaatgac acaacttctc gtatttatct ggcacaacat ataaatcgat caaaaaattg
     9781 ttgcgtgttt ttttgaaaat tggagtttca acttgattac aaaatttaaa tttagaagtt
     9841 gtgaaaacag cgagagacag tttaatattc aaaatataca taggctactc gaacgctgtg
     9901 ttttttagta tactagtaag aaacgaaggg atagactcat tccaataagg aggatttcat
     9961 gaagacaatt ctttttgctc ctgcagtatt caatttagct gagacaacac gaatgattga
    10021 ggttgccaaa gctatgaaag accgttatta ctgtgtattc tttggctttt ctaaaacttt
    10081 tgctcctttg atcacagaaa gtggctttga gtatcattta ctagcgccga tccttacgaa
    10141 aaaacaagaa gaacaaatca taaaatttga ccagttgcgt acacttaaaa acccttttac
    10201 caaagaaatg gttgcctctc gtatcactag tgaacagagt ctgattgcta agtttgagcc
    10261 tcgactgatt gtgacaggtt cgaatgtgac aattttttta tcggcccgca ataaaaagat
    10321 tcctttagta tatgttaaac cctatgcttt gagtgaacct ttttttcaat cagagcatgc
    10381 gctgatgccc cacgagttac gtttcttcgg ccctttaagc aatccccttt ggcactggac
    10441 aaaaaagcgt atagtaaata cccgatggat ccccaatgcc ttcaaacacg ctgcacaaaa
    10501 agaaatgctt cctttaccaa aaacaagttt acaaatgatg gatgctgatc taaatctcat
    10561 cacaacgcct aaactatttt tggaagaaga acagctgccg gaaaattatc gaatcgttgg
    10621 tccaattttt gctaagctca gtacttcctt gcctgaagaa gtgctaactt ttatacagcg
    10681 aaagcaacaa gaaagaaaac gtattatcta tttcgcaatg ggtagttcag gcaatccgcg
    10741 tatgttacgc aaaattttgt tcttcttaca aaaacaaccg gatctagcgg ttattgcacc
    10801 tattgactat ctgcttgatg acattcaatc tgactcagaa gatcttttaa taacagaata
    10861 tctgtctagt catcttttgc atgaattcat tgatttttct tttatccacg gcggggaagg
    10921 gaccgtccaa actgcctgtg aatccggcaa accgtttatc ggtgtgggga tgcaatttga
    10981 gcaaagctgt aatattgact attgtgtaaa atacggaaac gcaatcgccc tcacaaaacc
    11041 agtgactgaa agaaaactgc agattgctct gcgcgaagtt tcttcaccca agattcgtca
    11101 gcaggcacgc acgctgcaag ccgtttttcc aataaatggt ccacaacaag caattcaaga
    11161 aatcgaaacc ttcctgagca accacccaat atagtttcaa gtagaaaaag cttttttaat
    11221 agagactcat cagtaatttc gctattcgag acccagacag tgaaaataca aaaaaagtaa
    11281 atagtggatt gaaaagttag agttacttaa attacggtgt ctaaaaaaca caaaatcatc
    11341 cttaaaataa aagatctgac gctatccaaa tgagcatagg tagcgtctgc catttattct
    11401 tagtcaaatt ctttgacacc tttctctggt tggatcatct caacgtttct tcaaaagaaa
    11461 gaccataata attaggcaaa tacattttct taaaatgtgt catatagatc tagaagcatc
    11521 gctttgtaga gatttgtgcc gctagtcccc tcaacaatta ctaaatcaaa tgtaggattt
    11581 accactttat ctttgataat caacctccct cttctgttga gacaatgatc tcgcaatccg
    11641 atgcatactc ctatataaac tgccagcaat ccccacatat tgagtagatc atgtcagaat
    11701 gttctatgtc gtcagcaacg atacactaaa agtcacgttc accatgactg attgctattc
    11761 ccagcacaat tatttctata cacgtttcat gatttgaata aacactcaca tcatacatat
    11821 taccattcat acaccatact gcagccccta ctatatggtt gtaagctccc aaatctatat
    11881 ttgacttgat cactttggta gtagatctac taattctaaa tccattgctg ataaaatttc
    11941 cattttttcc tccttctttc tgccaaaaca gttaaaacaa taataatctt tatgtaaaaa
    12001 agggctgtga caaaagcctt gtcacagtcc catattccga ataaacggtg gtcaaaagct
    12061 tgcatctgcg gtaataagtt caaccaaacg aaaaataagc caacatttca caaaaattta
    12121 agaagcaatt ttcgtaaatt tcttcttatt tcttgaagct aaccacttct gttacaacca
    12181 cttttagaac atttaaagct tcgatgtgac aaaccatcta tgctgctcga tcataaaatt
    12241 gatagcaaaa attccacaaa gtacatatgt cccccatgaa aacaaaacat tcatatgcgg
    12301 tacaatgatc tttggaagaa tcacgatcaa aaatgatgcc agtgcccaaa tccatcgatt
    12361 gatttgaata tgatatgtag ctagcatatt caatataagg tgactagccg ttaataaaaa
    12421 gataaaaaca aaaaataaaa tcagccaata cattgtttcc caccttctaa aaaccaaact
    12481 tagacagtct ggacttcgtt ttctccacga ctttgttcat ctgacagcat ctttttatca
    12541 taagctttta caaatggata atagataata aatgctatca atgtgttgat catgatcaag
    12601 ataacaacac gccagtcatt tcctgctgaa agataaccgc cgattggagc tggtaatgtc
    12661 catgttggca aaacagtaaa tctattgacc atttctaatt tgactgcaaa ataactgata
    12721 atagtcaaaa ctaaaggaac taagttgaac gggatgaaca tgtaaggatt caacatcata
    12781 ggagctccaa aaattatggg ctcattgata ttgaagatac caggaataat agaaaaacga
    12841 ccaacctctt tcaagaattt tgatttacag aagaatataa acaaaacaga taccacgatc
    12901 gttgctcctg caccaccaat tgttaccgtc cattgataaa actgttcagg agcgatatag
    12961 ggtaattggg tggcagctgt accatctgcc aatgcctttg cattctcatc caacatgatc
    13021 agccacattg gacgaacaac agcccctaca atactcatgc catggatacc aaatgaccac
    13081 aacaaatgga tcaaaaacat tggtagtaaa acaccaagca aattattccc taaaatattg
    13141 gttaaaggct cgaataacga taataaggca gcgtttacat caaagcccgc gacgcttcga
    13201 atcagccaag taattgttaa aatgactgca cctggaatca atgcctcaaa actacgggca
    13261 acagcaggtg gcacttgttc tggcattttg atagtaatat ttcgattttt aaagaaacga
    13321 tatacttcca ctgcaaaaat catagataag atccccgcaa acatcccaga tgctcccaaa
    13381 gaactcatag gtaagaccca gccaagaggt gcctcttcag gagttaaaac gtttacagga
    13441 acttgtaaca taaagaacgt tcctaaagat aatataccac ctgtcacact atctaatcca
    13501 tatgatttag ctagatccga acccataaca aaagttgcat aaatagccat caatcccatc
    13561 gtcatcctaa agggtaaaac aatcgttgcc gaataagggg caataaattc attccatcca
    13621 ggaaacggta aattgataaa gaccataaaa aatgtcccaa tcaaaatcaa tgacaatgtt
    13681 gaaacaaccc cattacgaat tgctaataaa tgacgctgtg taccaatttt tgccattggt
    13741 gggacaagtt tctcttcgac ccatttcatc agcttttcca ctaaaaccac tccttttcaa
    13801 taaaatatga accattcatt gcatgacaac gcaaagaatg ttcataacta attatataat
    13861 taagaaattt tatttcaata tattctctta aataatcaaa tttattttta agaatcctga
    13921 aaataagttt tctaaatcat aggagcaaca aaaaaattcg ataattaaca aaaatcaaaa
    13981 tgattaaatc tagtgattga aacatatcat ttcaaaaaaa ttttgtaaat tcacccataa
    14041 atatcaccct atttaacttt tttcatcgta cactaaaatt caaaacaact atctttaatc
    14101 tttacttctt ggttttgttt taataaattt ccttcaaaaa tgcctggaga aactacaatg
    14161 atcaccgtat ctttttgtgc ttgctccaac ttcttcaaat ctataatagc taacagtgtc
    14221 ccttctttta cctcctggtt actttcaacc cgtatctgaa acggacttcc ttcgagttca
    14281 actgtatcta aacccatatg gatcagtatt tttctgccag attcactttc gatcgttatg
    14341 gcatgtcggg taggaaatat attatcgatt attcctttaa ctggcgcatg gatatatcca
    14401 tcatgattcg ttatagcgta tccaactcct atcatttttg aagaaaagac ctcgtctttt
    14461 actgctgtta atggaataat ttgaccacta gctacagctt ttagaatgtt tcttgtttta
    14521 aagaaatgca ctttttatca cctctacttt aatgctggcc aatatttttt gtttactttg
    14581 atcatttcat ctagtatttt ttttgcgact ctggcatttg gtacggtttg attcaatgta
    14641 aatgcttgta aagctttttg ataagaacct tcaaagtaag catctactag taacttctct
    14701 actgcaactt gtgcctccat caatccttta tgaaaatcac tgattggttt tcttagtgta
    14761 attggctcca caccagttgc accaacatat gccggtattt caacaactgc atctgaccgt
    14821 agattcggaa tcgccccttg attaggaacg attagcataa agcgacgacg atcatcattc
    14881 aacaaggaaa tagctatatc gacgatatat tgtccatgct ccccaaaatt aaagttcaat
    14941 acttcttcca caataccgcg gcgtatttta tcagccattt ctttggtatt tttctctcgt
    15001 ccatccatga ccatattagc acgtgtatac tctttgtcag tatgctcaac gaccatatca
    15061 ggatataagt aatactctaa gtaattattc gggatattac ttggaaaatt tttcgtgatg
    15121 actgacatca tttgaaaggt tttttgccaa gttttatctc ctatattaaa atcggcgact
    15181 tgcatttctt ttgttattag tttttcaata atctctggca taatttctcg ttgcaattct
    15241 ttatcataaa tcgatgtata ccagccaaag tggtttaaac cataatacgt aacgatccaa
    15301 ttcttacgat cataaccgta attcactgca atcgtttctt caattgaaat agtcatatca
    15361 caggcattaa tcatttttac attcggatat ttacgacgaa cagcttcaga aacgatcgac
    15421 tctggatttg tataattcaa aatccatgct ttgggggcat agtcttgtat atgatcgaca
    15481 atttccaata aacctttgat tgagcgcatc ccataagcaa atcctccgag tccacacgtt
    15541 tcttgtccta ctaatccatg tttaagtgga atcttttcgt ccttttctcg catctccaat
    15601 cctccaacac gaatttgtga gaaaacaaaa tcgcatcctg taaaagcaag ctttggatca
    15661 tccgttgcac gaatgcgaat atttgaaaaa ccttttcttt tcagcataaa tttaacaatc
    15721 aagtacatat ccatatttct ttcttcatca atatcatata aacgtatttc agataatggc
    15781 aaccgttttt cattgttcaa aatggcttga acgattcctg gtgtatacgt actcccgcct
    15841 ccagctatag tgataatctg ttttttcatc atcatctttt cctttctatt cttctaataa
    15901 tttattgaat tctgccataa cataagtcac ttctaagccg atgataattt gataattttt
    15961 ttcagttggt ttaatgatcc cgcttatccc taattgcttg attcgctgtt cctcgaccat
    16021 ggtgggatcc actacctcta cccgtaggcg tgtattacaa ctatagacat ccaggatatt
    16081 ggcttttcct ccaaacgctt gtagcatttt tgttgccata tattcataat ttttacttgc
    16141 taaccctaac tctgtttctt ctaaggttac ttcattgcca aactctgcct tagcttcacg
    16201 tcctaatgtc tgaatgtttc tcttattgat caaataataa aaagtaacgt aatacaaggc
    16261 aaaaaagaaa attccgattg gaagaatcaa aatagcattg gtagcgattc gaaaattgat
    16321 gagataatca acaatacatg ctccgaatgt aaaaccaatt cgtatattga ataagtaaca
    16381 gacaatccct gctaaaccgg caaaaagtgc atggatcacg tacaacatag gagaaagaaa
    16441 catgaaacta aattcaacag gctcagtgat atttgcaata aatgaagttc ctgcaccgct
    16501 tcccatgatt cctttgacca tctctttatt ttctttataa gctgctttat agatagctag
    16561 acatatagct ggtataccaa acatcattac tacaaaaaaa actgataaaa acagtccagc
    16621 ctgaggatct ccgttgataa agcgtgtcat ctctcctctg aaaacttcct tgctaggaga
    16681 tgtatagctt cctaaatcgt aataaatata cgtattcaac acatggtgca gtccaaaggg
    16741 gatcagcaaa cgattcaaaa acataaatat ccccacacct aatgctccca tatcaacgag
    16801 cagttttgca aaattatcaa tcccaatttg gatgagcggc cagataaagc ccaaaaaaac
    16861 agctgcgatt gtttgtagaa tcatcaccat agtcaatggg aatttttcgc ccgcaaaata
    16921 agaaaaaact actggtaatt tttgttcttt aaagcgatta tacgtccatg ccgctactaa
    16981 accggaaata attccaccaa aaatccccat attcacttct gggtccataa tttctaagcc
    17041 ttttttcaag gtagcaatcg ctaaaaaacc agtaagtgca ggtattcctt tgtccttatt
    17101 tttgacaaaa ccgattgcaa ctcctatagc aaataagata tccaagtttc caaaaacggc
    17161 atttccagct gcaccaatta ctgggatgtt cagcatatcg tcagctgata aacgcatgat
    17221 caatcccgct aatggcatcg ctgcaactgg aagtagcatc gctcttccaa gcttctgcac
    17281 atttgattta acattcattt ttattcctcc tctattaaat atcctcatat gttactaact
    17341 caatcaaagg atcatttttc actttttgag caaatgctga atgaattaaa aattcgactt
    17401 ggtgggtacg aggatacaca aaacttgaac catttaacaa ttcctgatcc aaatacgctg
    17461 cgtgacacat gacttcgatc gttttatctt ctctcaatat catgagtaaa cttgccaaat
    17521 attcttccac actcgtattg atttgtcgtt ttttttcatc agtttctcct acgacatcga
    17581 aacaaggctc aaaataatcg acatgacgaa cttcatcttt tcgttcaaag ttgctccgaa
    17641 ccggcaaatc atattttttt gctaatgtaa caactacctc ctgattttca tccaaagtat
    17701 gagtatgatg atgactgtct aaatgagttg gtatgatccc cgcatgataa attttttgaa
    17761 tctgagcatt ccattcctga tacagttgat ctttgtcaat gttaaatgga tgctgataat
    17821 aagataagga tctgaactct ccgctttcga taagagtatc gacagttttg aggagtggtt
    17881 tgccacacgt caatgttaaa tggactccta cacctagcct aggcatttcc tttcttagtt
    17941 tcacagcatg ctcgaaaccc ggcatatttg ccatcaatgt agttgatgtc aaaattcctc
    18001 gctgataagc atccataatc ccataattca caccatggct atagccaaaa tcatccgaat
    18061 taaaaatcac ttttcccatt tcattctcct tcttttttct actaaaatag gcgcaaccaa
    18121 ataatcatag tccatttact atgtgtcatt tggttgcgcc tatagatagt tacgccctta
    18181 ttttaataga ttcttgctag atgaattgcc aaatagatga tttcattttc ttgaatggca
    18241 agttgaaatt gctgttcgat gatatcttta atctttagtg ctgtacaata cgctttgcta
    18301 tgtttaaatg tttcagtaaa aaaaccaata ttttctactg gcgtttcttc ctgataaatc
    18361 aagcgtttaa ccaggaattt gatatgaacc attaagcgat gataagtcag tgattcttta
    18421 tcataaacgt ttgaatagga tttttctaga tgggacaaaa tcgtggtcgt catttctact
    18481 atttttaagc tctttaaact attaggctct cctagctcag cattaatgat atgcatagtg
    18541 atgaatccta cttcatttga ccctaactcg gtttgatatc gatgattgat tcgctcgacc
    18601 acttctactg ctatatcata ttcatttgga taaaggaatc tcatttctgg caacataatt
    18661 gctgtgacat ctctatcttc tctttttcgt tgaatagcaa aataaatatg atctgtcaat
    18721 gtaatataga tatttgaatc taatggagag gagatcctag ctgttatcag ttcaatcact
    18781 tcttccgcaa aatccataaa ttcgattgga atctgcgaga ctatctcact gaatcgtttg
    18841 atcttttctt tagaatctaa tacaaataat ttgtcagcat cctcaacatc aaatggctta
    18901 ctcactttag cagaaaagcc aatccctttc cccatcgcaa ttttttctat tccatcgtca
    18961 tcgatcaata cagcattatg attcagcttt ttcatcacat acaccgttaa ttcacctcct
    19021 ctatatgata aaacaaaaca acttgcagac tccttcatac atatttttgt ctccaaacaa
    19081 tttttaaaga caaaaaacga cctaagaaag aaccctttct taggtcgtgc ctaattgaat
    19141 agtagcaccc tacataattt acaaaaatag tttaacacat aataaaagcg ttatcaatat
    19201 cttttttact cgttttatga taatacttgt atcgaattga tttggaggta attacataaa
    19261 aacaaaaaaa atatagcaac tctctataag aaagttgcta tattgataat tataccggcg
    19321 gccggggtcg aaccggcacg tcctcaacgg acactggatt ttgagtccag cgcgtctgcc
    19381 aattccgcca cgccggcaaa tttaaaaata ataactgggg tagctggatt cgaaccaacg
    19441 catgagggag tcaaagtccc ttgccttacc gcttggctat accccaagaa taaagggcga
    19501 atgatgggaa tcgaacccac gaatgccgga gccacaatcc ggtgcgttaa ccacttcgcc
    19561 acattcgcca tcataaaaga tgttatttaa tttctcaata aaaaactaag atgaagacat
    19621 cttagtcaat accggcggcc ggggtcgaac cggcacgccc tcaacgggca ctggattttg
    19681 agtccagcgc gtctgccaat tccgccacgc cggcaagttg atttattagt actaaggcgg
    19741 taaccggatt tgaaccggtg atgaaggttt tgcagacctc tgccttacca cttggctata
    19801 ccgccattat actattatca atctataaaa taaagaataa attatagaaa ctggggtagc
    19861 tggattcgaa ccaacgcatg agggagtcaa agtcccttgc cttaccgctt ggctataccc
    19921 caagaataaa aagggcgaat gatgggaatc gaacccacga atgccggagc cacaatccgg
    19981 tgcgttaacc acttcgccac attcgccatg gcaggggcag caggaattga acccacacta
    20041 acggttttgg agaccgctgt tctaccttta aactatgccc ctataaaatg gaggagagtg
    20101 gattcgaacc actgaaccct aaggaacgga tttacagtcc gtcgcgttta gccacttcgc
    20161 tactcctcca tggtggcgcg ggacagaatc gaactgccga cacacggagc ttcaatccgt
    20221 tgctctacca actgagctac cgcgccatat tttaaaatta ttaattgaat aacaagaacg
    20281 acggttccga cgggaatcga acccgcgatc tcctgcgtga caggcaggca tgttaacccc
    20341 tacaccacgg aaccagttca ttatggaggt taacgggatc gaaccgctga ccctctgctt
    20401 gtaaggcaga tgctctccca gctgagctaa acctccaaca ttcaattaaa atgacccgta
    20461 cgggattcga acccgtgtta ccgccgtgaa agggcggtgt cttaaccgct tgaccaacgg
    20521 gccaggtaaa tttgctacgg agagtaaggg attcgaaccc ttgagacagt ttggcaccat
    20581 ctacatgatt tccaatcatg ctccttcggc ctctcggaca actctccaga gtgaaagaag
    20641 ctcaatactc tttctcaaaa tgagaactcc ggcagtagga ctcgaaccta cgacatcatg
    20701 attaacagtc atgcgct
//
LOCUS       DACDUU010000055        20612 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.055, whole genome shotgun sequence.
ACCESSION   DACDUU010000055 DACDUU010000000
VERSION     DACDUU010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.055"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            72..506
                     /locus_tag="IWL30_001971"
     CDS             72..506
                     /locus_tag="IWL30_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9706710.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(584..1117)
                     /locus_tag="IWL30_001972"
     CDS             complement(584..1117)
                     /locus_tag="IWL30_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAP9706711.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(1211..2389)
                     /gene="mutY"
                     /locus_tag="IWL30_001973"
     CDS             complement(1211..2389)
                     /gene="mutY"
                     /locus_tag="IWL30_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAP9706712.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2382..3179)
                     /gene="recX"
                     /locus_tag="IWL30_001974"
     CDS             complement(2382..3179)
                     /gene="recX"
                     /locus_tag="IWL30_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAP9706713.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            3286..4668
                     /gene="rlmD"
                     /locus_tag="IWL30_001975"
     CDS             3286..4668
                     /gene="rlmD"
                     /locus_tag="IWL30_001975"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP9706714.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4842..5420)
                     /locus_tag="IWL30_001976"
     CDS             complement(4842..5420)
                     /locus_tag="IWL30_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAP9706715.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5742..6212
                     /locus_tag="IWL30_001977"
     CDS             5742..6212
                     /locus_tag="IWL30_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAP9706716.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(6320..7546)
                     /locus_tag="IWL30_001978"
     CDS             complement(6320..7546)
                     /locus_tag="IWL30_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAP9706717.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(8300..8500)
                     /locus_tag="IWL30_001979"
     CDS             complement(8300..8500)
                     /locus_tag="IWL30_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9706718.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8634..9242)
                     /locus_tag="IWL30_001980"
     CDS             complement(8634..9242)
                     /locus_tag="IWL30_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAP9706719.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(9327..9797)
                     /locus_tag="IWL30_001981"
     CDS             complement(9327..9797)
                     /locus_tag="IWL30_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAP9706720.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9845..10759)
                     /locus_tag="IWL30_001982"
     CDS             complement(9845..10759)
                     /locus_tag="IWL30_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP9706721.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10905..11708
                     /locus_tag="IWL30_001983"
     CDS             10905..11708
                     /locus_tag="IWL30_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAP9706722.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11886..12833
                     /locus_tag="IWL30_001984"
     CDS             11886..12833
                     /locus_tag="IWL30_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP9706723.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12901..13194)
                     /locus_tag="IWL30_001985"
     CDS             complement(12901..13194)
                     /locus_tag="IWL30_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAP9706724.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(13222..13887)
                     /locus_tag="IWL30_001986"
     CDS             complement(13222..13887)
                     /locus_tag="IWL30_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAP9706725.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(14027..14659)
                     /locus_tag="IWL30_001987"
     CDS             complement(14027..14659)
                     /locus_tag="IWL30_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9706726.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(14666..15733)
                     /locus_tag="IWL30_001988"
     CDS             complement(14666..15733)
                     /locus_tag="IWL30_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9706727.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(15730..16461)
                     /locus_tag="IWL30_001989"
     CDS             complement(15730..16461)
                     /locus_tag="IWL30_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAP9706728.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(16630..17109)
                     /gene="greA"
                     /locus_tag="IWL30_001990"
     CDS             complement(16630..17109)
                     /gene="greA"
                     /locus_tag="IWL30_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAP9706729.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(17245..18420)
                     /gene="mltG"
                     /locus_tag="IWL30_001991"
     CDS             complement(17245..18420)
                     /gene="mltG"
                     /locus_tag="IWL30_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAP9706730.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            18624..19793
                     /locus_tag="IWL30_001992"
     CDS             18624..19793
                     /locus_tag="IWL30_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP9706731.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(20060..>20612)
                     /locus_tag="IWL30_001993"
     CDS             complement(20060..>20612)
                     /locus_tag="IWL30_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706732.1"
                     /translation="LEKFSYTRRRNFILLLILAISILNYSSVVNMVKEMNFQGTYSNE
                     QMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPNKDEITFTFKSSKG
                     KTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTIRYSYTFFSKISLF
                     ISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT         6260 a         4091 c         3291 g         6970 t
ORIGIN      
        1 tttgaaaact tgccatactt tttatttgtt ttattttcca gtatactata cgaaaggaag
       61 gtggctagac catgaaagtt gtacaaacaa aagatacaat gagcaatatt tacttagatg
      121 ctgtacggat caggcatcaa gtttttgtag tcgaacaggg tgtgcctttg tctcgagaaa
      181 tagacaaaga tgaagcccat tgtatccact tcgtcctgta ctcagataaa aaagaaccgc
      241 aaggaactgt acgtcttttg cctttagaaa acggaaaaat gaaattacag cggatggcga
      301 ttctttctga atacagacac caagggttag gaaaaatatt gattgaagaa gcagaaaact
      361 ttgctaagaa tcaaggctat aatacgattt tattaggggc acagtccaca gctgaaactt
      421 tttacgaaaa actaggatat acagcttacg gcgatccttt tgaagatgct ggcatgcctc
      481 atatttatat gaaaaagact ttataaaaat ttatttcgaa aaaagcaaaa aacagcaagc
      541 ttttgagaaa aatctctcac tggcttactg ttcttttttg tgtttactta cgcgacaatt
      601 gcttgtaacg gttataccaa atatcaatat agccttccga gaacgggcca tctccattat
      661 tgatccagtc aaccagaatc ttcacatttt cttttaggat gaagtcaata tcgttcgggt
      721 aattcatcat tttgctgtgc atctcatatt catcgacatc caatagtcgt ttctcgccgt
      781 caggaaatac cttgatatcc aagtcatagt cgatatattt caaggcctca tcgtccagca
      841 aaaaaggtga agctagatta caataatagg aaaccccctt ttctctgatc attgctatta
      901 cattgaacca gtatttttta tgaaaataaa caatcgctgg ttcgcgcgtt acccatcggc
      961 gtccatcaga ttcagtgact aatgtgtgat cattcacgcc gatcatagag tgttcgcttg
     1021 tttttaatac catggtatct cgccacgttc gatgcaattt gccgtcgtgc ttgtaacttt
     1081 ggatcgtcac aaactctcct tcttttggaa ctcccatagc aacccttcct taagcagcat
     1141 tttgtgcttt gtgtttgggt gttcattaca ccgacatcat aattattata tcaataaaaa
     1201 tgagctttgc ctagaaagga ttcctattgt ttcgattttt ctctaactga tctaccagtt
     1261 tcatttggac cttaggaaaa acgaccgaat caaatgcatc aggcttcaac cacttcgaag
     1321 ttttgttctc ggtaaattct tgttctgctc cctcggttgc ccgtccgtaa aaaaggagaa
     1381 catgccattt cagatgacta aaaatatgag taacttctcc taaatgtcgt gtctgccaaa
     1441 caaagagttc ctgcttttca aatgggagat tttgctcttc ttctgctact aggtcatcaa
     1501 acagatcgat ttctggcttt gtttcccatt cttttttcaa tcgctcatac tcctcttgtg
     1561 tcacttccat cattggaaat gtccacatat tcgccagcaa tttttggcta tctctttctt
     1621 caaaataata cgctccagaa tgattttgaa gagctgcact gatataataa acgtcttttg
     1681 gctttgcttt ttttgttttt acaggaaaac tcgtttggat tcctcgtttg ttagctaaac
     1741 aaaaggcttg gatcggacaa gtctcacact ttggagaagt tggtgtacag atagctgaac
     1801 ctagatccat cattgcttgg ttgaattcac ctggatgctt ttcatcaatg attttccgca
     1861 tcgcttcatc aaagattttc ctgctagaag cttttgcgat gtctgcttca atgcaaaaca
     1921 atctgctgac tactcgcatc acgttgccgt caactgcagg ttctggaagt ccaaatgcga
     1981 tacttgcaat cgcgcctgtc gtatatggtc ctattccctt caatgagcta atttcttcag
     2041 gcgtttgcgg catttcccca tcaaattctg acatgatctg tttagcagca gcttggatgt
     2101 tccgggctct tgagtaataa ccaaggccct cccatgcttt taaaagcttt tcttctggag
     2161 catttgctag ctcttcaatt gtaggaaacc attccatgaa acgataaaaa taatcgatca
     2221 ctgtgtctac tctggtttgc tgaagcatga tctcagagat ccagatacga taaggatcac
     2281 gattatagcg ccaaggcagg tttctttttt cttgttcata ccattgaatg aactgatctt
     2341 gaaattcttt tgtttcttta tctgtccatt tttcccattc tttattcact ttcctcttcc
     2401 ttttccgtaa tgaatcgctg gatcatatcc aaatcaaatc ctttttgata taagctttgc
     2461 tttactttta gatttctttt tttaggttca aaccttaaat ttcttcgcca tagtttatct
     2521 ccttgtttga ctaaataatc atattctatt tcttgatctg cttcttgcgg aagatcagct
     2581 atcgcttgtt ggatcacttc ttgagaaaag cctttcgtca tcaatgtttg ttggattttt
     2641 ctcaataatt ctttttggct ttttccgtga ttttttccaa acgctttttc ggctgtctga
     2701 tgtgcatttt gaacttggtc ttcaaatgta tattggctga gcgcttgttc aatgatttca
     2761 ggtttcaacc ctttttgctg catctgctgc gctaatttct ttggtccttt gtcacttaag
     2821 cgcatattcg tgcgcacata actttcccca tagaccaaat catcgacaag gtccaattct
     2881 tttaagcggg ctacgatttt atttcgatct tcttgaagga tttccttttc ttttaagtat
     2941 gttcggattt ctttttccga tcgcaactga taacttaagt aattcattgc taggtgaaaa
     3001 ccgaaatcag ctccgctact cttcttgatc tcttggatag tttcttcgtc taattcccgt
     3061 ccttttaata atcgatagcg tactaataca tcttcagata cttgtaaagc ttctccttca
     3121 gataaatcta cgcgatagaa agggcctttg cctttactga ttctcgtaac ggttaacata
     3181 aacatcctcc tcgaacatat attctctatc ataccacaga aaatccccgt aatcaggtat
     3241 tatatgttag aataaaccac agaaaaaaga aaaagggaga tacttatggc agaaacaatt
     3301 gtcaaaactg gacaaacggt tcctttgaaa atcaagcgtt taggcataaa tggtgaagga
     3361 atcggttatt tcaaacggtt gatcatattt gttccatatg cactaccaaa agaagaagta
     3421 ctagtgaaaa tcacgaaagc aactcctcgc tatgcagaag gagaattaat aaaagtcaaa
     3481 aaaacaagca aagatcgcgt agtggcacca tgtcctgttt actatgaatg cggtggctgt
     3541 caattacaac acttagctta tcaagctcaa ttggatttta aaaaggatct gcttttgcaa
     3601 gcattggaaa aattcaaacc agcaggcttt cgatcttatc aacttttgcc gacgatcggg
     3661 atggatgagc cttggcatta tagaaacaaa gcacaatttc agttacgaaa aaataagcag
     3721 actcaaaaaa ttgaagctgg tttatacgaa gcaaactctc atcagttagt ccctataaca
     3781 gactgcttgg ttcaagaacc tcgaacacaa aaagtaatga atactgttgt tcaactattg
     3841 aataaatttg atgcacccat ctatgatgaa cggacaaaca gtggaatctt ccgcacaatc
     3901 atggttagaa tcggtgttaa aactggggag ttacaagttg tttttattac tcgcagtaaa
     3961 aaattcccgc aaaaaaacgc aatgatccgt gagatcaatg aacgactgcc cgaagttgtt
     4021 tcaatcatgc aaaatgtcca ggcacaaaaa acgtctctcg tgatggggga tgaaacgatc
     4081 cacttatggg gaaaagaagc cattgaagaa caaatcaatg aagtgacttt cgatctttct
     4141 cctcgtgcct tctttcaact caatcctcag cagaccgaat tgctttatgg cgaaggaatc
     4201 aaagcattag atgtccagcc taatgaaacg atcgtggatg cttattgcgg agttgggacg
     4261 atcggcttaa gtgtagcaaa acaagccaaa gaagtacggg gaatggatac cgttcctcaa
     4321 gcaatcgctg atgcaaaaaa aaatgcggaa agattaggat atcacaacac tcgatatgaa
     4381 gtcgggacag cagagcaagt tctgcctaaa tggttgaatg aaggttttca gcctgatggt
     4441 atcatcgtag atccaccacg tacgggtttg gataacgcat tgatccaatc attattgaaa
     4501 taccctccaa aaaaacttgt ttatatctcc tgtaatgttt ctacacttgc cagagatctg
     4561 gttaatttaa gtaaaaagtt caagattgaa tatttacagt ccgtcgacat gtttcctcag
     4621 actgcacgtt gtgaagttgt cgtgaagatg acgagactcg ctgactaaat gaaagaggtt
     4681 gtgaaaaagt ctgccctgta tcaagtaata agaacagcca aacaaaaata gctcttcatg
     4741 ttttatgaat actgtacttg tgaccgaaga gttaacagga ggacactatt tatttaaaaa
     4801 aagggttgtg acatagtttt tgtcacagcc ctttttgatt attattggat attcacgtct
     4861 atcacttgat aaaatgcatt tgtcgtatcg ctgatattcc atactgctaa aatcacatta
     4921 tatccttttc gatcctgcgg aagtgtgatc gtttggttga ctgtttttgg tggaacagtt
     4981 gctttgtcgt ctatttttgt gatcaattca aaatcactaa atttcaaagg ttgattagga
     5041 ttccaagttg gtttggtaat gtagtaatcc caagtacttg tccgatgttg tgctgttaaa
     5101 ttccaagtaa tatttaatgt tcctggatta accatgtttt tatgccatct gttagatgtc
     5161 tgttcatcta aaggctcaaa gccgctgact cctgcactag caatttttcc atcaatgaat
     5221 gtatttttgg tagcttcgat actttgcggt tcccattgtg ctcttccgac attttgattt
     5281 aagtttcctg ctgctgatgt tcctaggtat gcacggcttc ctggagaagt aatataacca
     5341 tgcgcaaaac ctggaatgct aaaaccgaac aaccctaaac taactaaccc aataaacaat
     5401 aacgtactaa tctttttcat gtagaaacct ccttcatttg ataaccacat tgtaaacgct
     5461 ttattgtatt aacacaaaca ttatgctttt ataattttat gttgaagtaa aaaaggtgaa
     5521 aaaagtaaga tgcagattat aacagaagta gacgagaaaa gtacaaatag aaaacatgag
     5581 aatcaaatca tatgatcttt ttcaaaaaca tcattcatat aaacaaatgt tcaacttttt
     5641 aataacaaca attatcaatt tttttcttac ttcacaaact aaatacaggt cttgctgtga
     5701 gccttcatgg taaactaata ttatagaaag gagttagtaa aatgaagatg ataaaagata
     5761 ttcgtttttg ggtttgtgtt ataggaattg tcatattagg ctttttatca gggttgctta
     5821 gcggaaatcc cggaaaatat tactattctt tgcaacttcc accatttgca ccacctagct
     5881 ggatatttgg gccgatgtgg accttactgt atatattgat gggtatttct ctttatttgt
     5941 tacttaatca caacaacaaa aaacagcgaa acaatcttgt cggtcttttt gtgattcagt
     6001 tcatttttaa ttttatatgg tctgctctat tttttaattt aagaaatatt ttcattgcag
     6061 caatcgatat tacactttta gttattttct tgtcagtgct tatgtatcag ttgtggctgc
     6121 atcatcgcct ggcaatgtgg ctcatgattc cttattattt atgggtacta ttcgcaactc
     6181 tattgaatta ttcaatttat tttttaaact aagcgctaaa aagagacgtc ctgtaattta
     6241 acacatagaa aaagtgccac ctaagttaga ttttttgtct aatttaggtg gcactcttta
     6301 ttaaccaaac tatttttttc tagtacccac ttgttgttcc atactcatta ttctggtcat
     6361 aaccataacc attttgttcc tgtccagcgt aaggatcata actttgagta tcttgacttg
     6421 gtgtatagta ctgacttgta tcttctgttt gctgataata ggtattcgga tcttggtact
     6481 gatcatatgt gttttctgaa taggaagaat aatcggtgta cccttcacta ttcgatccat
     6541 caccgtttgt cgaatcgcca tcttccgaat catcaaagaa catcattgaa gcattctcat
     6601 ttttcaaatt aggtaatgta tcgcttgttt ttatatttaa ttgtttcttc agttcatttt
     6661 ggatcgaaag aagatcattt tttcctaaaa tttgatagta gatatcattg atcatttgtc
     6721 cttcgccttg caactggtct tgtttgattg tttcaaacgc cggggtgtag ttcgtcgcta
     6781 catcaagcat gtcatcccaa tctaagtccg tcgtcacatt gttctcaact gcttttaaaa
     6841 tttttctata attgcttacg ccatctaaac ttaaaacttt attgacgatt ttcgtcacta
     6901 cttcccgctg acgacgttgt cgaccgatat cgccttctgg atcttcgtgt cgcattcgag
     6961 aataggctaa acctttttct ccattcaatg tttgttcacc tactggtaca tggacaccat
     7021 ccaaagtaaa gtcaatttta ttattcactt tgatgccacc gaccgcattg atcaaatctt
     7081 ccatgccatc caaattgatc gttacataat gatcaatagg aatatcgagc agttgctcaa
     7141 ttgaatccat ggccatttcg acaccaccaa atgcgtaggc atgatttaat ttatctaccg
     7201 ttccataacc tacgatattc gtataaatat ctcggtccaa actgatgatc gttgattttt
     7261 tttgtttcgg attaactgtt actaccatca tcgtatcaga acgtccttgt tccgttcttc
     7321 ctaatccacc ggtatctaaa cctaatagca aaaccgaaaa aggttctttg tcatcaatgc
     7381 ttactctctc agaacgtttg gaggatattc gattagagtt tttagaaatt ttgtttactg
     7441 tttggctcgc ttctcccata aatctgattc cgtatgctga taatccggca atcagcaaaa
     7501 cgaatacccc taacaaggtt aatagaactt tttgccatcg cttcataata cacctctgtt
     7561 tcacattttc taaaatattg tctcatataa ctgttcaatc agcaaattca ctttttaatc
     7621 ataagaaaac tttaaaaaac atttttgtgt aacgattatt attttttaaa atttctcaaa
     7681 attcttaact atcataccat accgtgaaaa agatcgaaag accgaataca cgcagttcgc
     7741 aatcgtttta tcttttttta tgtatttttt ccagaaaaag cgaacaacaa ggtattttcc
     7801 aaataatcgt gcagtaaaat gatcgcacat acctttccca tctttttaac agtctttgcc
     7861 taccgttcag attatcattc gatttcacga caatttcttc ctatgactgg tccttttgtt
     7921 aaacccatct tctattcgtt ttatttccag ctcactgcat ggctcttaaa atgactcgaa
     7981 ctagtgattg cattaccatc atcttttaat ggatagatat gctcctcttc aaagtaaaac
     8041 gattttcttt taaagtaata gaattgacat ggtaatgagt atgtatacta gtggtttcac
     8101 aaatggtctt ttattcaaca tccaaaaact gaaatttctt taaaatccat tactaacgaa
     8161 gaaaatgttc cttgcttgat aattagttag gacatgtcaa gtgaacaaat gcatttactt
     8221 agccaaaaaa tctaaaaact tcataaatag acaaaaagac acgactctct aagaagtcgt
     8281 gtctttattc aacaaattat tataatttag tcacttcagc agcttgaggt ccgcgtgcac
     8341 cttcaacaat cgtgaattct acttcttgac cttcttctag gctcttgaag ccgtctcctt
     8401 gaattgctga gaaatgaaca aatacgtcat ctccgccgtc tactgtaata aacccaaatc
     8461 ctttttcatt gttgaaccat ttaaccgttc cgtgttccat aaatgtatat cctccaaaaa
     8521 agttcttgca agtaaccttg caagaacatt atacccaatt tagaaaacgc tagcaagctt
     8581 tcgctctttt attttccaaa ttatttttaa gactgatatg acggaggaaa tggttatttt
     8641 gctgcttcga aacgtttgtt tacttcatcc caattaataa cattccagaa tgctgcaata
     8701 taatctggtc ggacattttt atatttcaaa taataagcat gttcccaaac gtccaaacct
     8761 agaattggtg tctttccttc catcaatgga gaatcttgat ttgcagtaga ggtaatagct
     8821 aattttccat tttccattac aagccaagcc catccagaac caaatcgtcc agcggctgct
     8881 tttttgaatt cttctttaaa agaagaaaaa tcaccaaaag cttcattaat cgcttctttt
     8941 atttctcctg taggttcgcc accagcattt ggtgccatga tttcccagaa aaatgaatgg
     9001 ttagcatgtc cgccaccatt gttacgtaca gctgtcttga tatctgttgg aatagcgtcc
     9061 atatcagata ataattcttc tattgttttt tcgcctaatt ctgggtattt ctcaattgct
     9121 gaatttaaat tcgtcacata agtattgtga tgcttatcat gatgcagatg catcgtttct
     9181 tcgtcaatat aaggttccaa tgcgtcatat gcataaggta gatctggtaa tgtgtaagtc
     9241 ataaaaaaat tcctcctcaa tgttttctta ataatgtcta cattacaaag agtaacaaag
     9301 agaaggaaat tcttcaattg ttatgcctag tcgtcttttt cactttgacc tacttcttct
     9361 tcaaaaacct cagtttcttt aaaaataaac aacttgctga aaatatagtt cgcaaggata
     9421 acaataattt gagtgatgat tttcgccaca tactcattgc tgttcataac attgatcaac
     9481 aataccattg cccccatgtc caatccgtaa gaaaggatac ggtagaagaa gaacttaccg
     9541 cattcaataa gtgcatgtgt aaaattcggt gattttgaat ggaataccca aagtttgttc
     9601 gttacaaaag caaacaatac agaacaaaac cacgaaatag aattggataa ggccatactt
     9661 aaatcaaaat attggcgtgc gagagcaaaa ctaacaaaat ttacaatcgt agccaaacca
     9721 ccaaaaaaga gatagactag tatctcccaa tatccttttg cttctaaata tccttttaac
     9781 tgtcgatact gcttcatgag ccaacctacc ttattaaaaa ttcttattta tcatactcta
     9841 tatttcaata actttcatct ttatttaaaa aaattaattt tttttgatcc tttgcctttg
     9901 tctctgcttg gatcgtcaca tgtgtaatac catattttaa ttttaataaa gaaattatac
     9961 gaggatatat cttttcaatc tcgcttagta gctgatcttc aaaatcaata tgtccgtcga
    10021 aaataattat cttttcgcta tactgccaaa tatggacatg atgaacatcc tttaccccat
    10081 cgattgcttc aatttcttgt tccatttttt catagtcgat agcaggtgca ctttgtatca
    10141 aaatagcggc agcttttttg atcaccatta tcgcttctat caatatatat atcgaaatga
    10201 tcagtgtaat aactggatcg atccagtaga tatcgaaaaa acggatcaaa attgctccga
    10261 taatcacact taccgaagac agtgtatcac ttaacaaatg caaataacta gattttatat
    10321 tcaaactttc atctgcttct tgtcgtaata ataatactga gaacaaattc gcgagcaatc
    10381 caataactgc tactgttagc atcaaatgac tatttatctt ttctggtgaa gtgaaccgct
    10441 tgaatgcttc aacggttaaa aacaaggata atatgatcaa tatgaatgag ttgatgaaag
    10501 cactcaatat ttctaaacgc tggtagccat acgtacgttt tttatttgct tcttttcggg
    10561 ccattttttg tgccccgtaa gaaaaaataa ttgccaatgt atcactaagg ttatggaaag
    10621 catctgaaac aagagacaaa ctccctgaca taatccctcc aataaattct gataaagtga
    10681 tcaccgcatt caaaaccact actccaaata gtttttttga tgatctctgt ggctgtgtag
    10741 gcactattcc ttcctccatt ttatttatat tggatagtct ttagacaaga ataacatttt
    10801 tttgatattt caccaatcat tcagctgttt ccttaaagca cttgtatttc acttgatact
    10861 tcgctagaat tgcaagtgat gacactacta gatgaggagt acttatgaca acaaaacaat
    10921 taattatcag ttcctttata cgattcaaag acctaaagga ggcgagaaat gtcccatttt
    10981 ggaaagccat tgtctatctt ttagttttga gtataattat ggctttgcca atctcttttc
    11041 aagtttttca agtcttaaac aacattcaat tggatgggca aaagattgct actcgcattc
    11101 ctgattttac aatccaaaac gggcagcttc aaaccgaaga aaaagaagga ttcatttacc
    11161 aaacaaactc cattatcttc acatttgatc cagagggaaa acgaacagaa caggacatct
    11221 ctactgattt gatggggaat tttgttagtg tcgggatgtt aaaaaacaaa cttatcatag
    11281 ctcttcctaa tacaggcacg acttctgctt tgttgaacaa taaccaatta gaacttccct
    11341 acaccaatga atctttaaaa aacttaacag gtaagcagct ccgttctttt ttaagtgaag
    11401 catctatacc actttggata aaagtcctga catttatatt ttctatttat cctagttttc
    11461 tgaatctgct gatcactttg ctatttacca acattgctgc ttttctctat gcacgctttc
    11521 gactaacaaa agctactttt cttgattgtt taaaaacatt gatctattca atttcattgc
    11581 ctactataat tgcaacaatc ttgatggtct ttcttccttc ttttgacact agcgccttca
    11641 ttgcttttgc aggaatcttt atttttgctc aagctgtcaa aggatgggca aaaatatcga
    11701 tcaattaaga aataaagaca actcgttatt tctacttcaa tagacccttt tagaggttgt
    11761 gacatttttt tgtcacagcc ccttttttat ttgaaaaata tttctttctc aaaaaacaag
    11821 caaggcgctt caaagttctt cttgtatgcg ctacacttag agtaaagaaa ggcgggaaat
    11881 taactatgtc agaaagaatc atttatgcag atcgtccatt tcgtgatgaa tttatcgaag
    11941 aaatccatac tatagcgcct ggttatatct ttaaaacaga attacaacca gaggatctac
    12001 aaaatgtaga gatcagtttg ggctggaact cagactacca agaaaaacta ttagcctctt
    12061 ctaccttgaa atgggtccaa tctatctcag ctggtgttga tcagctaccc cttgagactt
    12121 tttcaaagaa acatatttta ctttcaaatg gtagtggcat ccacagtgaa tcgattacgg
    12181 agcatgtctt aggaatcatc cttgggtatt ctcgtggtct ttttcaagca caaaaggcac
    12241 agagtgacat gaaatggtta ggttcatcgg tccattacca atctatccaa gggaaaaaaa
    12301 tactaatcat tggaactggc catattggaa aaatgattgc aaaaaaatgc aaagcattag
    12361 acatgaactg ctatggaatc aatacatccg gtcatccagc agaaggaatg gaaaagactt
    12421 tttctttaaa tcaactaaag gaagttcttc ctgaagtaga tttcgtagtc aatatcttac
    12481 ctctaacgga ggaaacaact gggctctttg atcagcatat gtttgaacac tttgatccaa
    12541 aatctgtatt catcaatgtt ggccgtggtg cttctgttaa gacacaagat ctggtgcagg
    12601 ctttaaataa ccagcaactt tcctttgcag cattagacgt ttttgaggaa gagcctcttc
    12661 cacaagatca tcctctttgg aaaatggaca atgtactgat tacttcccac attgcaggtt
    12721 tgacacctga ttttcaaaag aaattaatgg ctattttttt aaccaatctg aaaagctact
    12781 ttgccaccca tgagcttcaa accaatcaag taaaattgtc tgcaggatat taatagctga
    12841 aaacaatatt ttcaaaagaa aaaagagaag gtgacaaaaa ttgtgtcacc ctctcttttt
    12901 ttattgtttt ttaaagtgat aggttggctc ctccaggttg ggatcataat caacaagtaa
    12961 atcatatcct ttaaagaacc attcatctgc ttcctcgata aaaaaaagtg tcccatcaat
    13021 ttccactttc tttacaggat gttctggctg ttcaactgac attccaatcg aaaaaccatc
    13081 atgaacctct gttttgccgt agacttttcc aaaaaaacgt attccgtaag tttctggcag
    13141 atcaatttct gagtcaaacc acttttgtgc ttttggcgtt accgttaatt tcatcccaac
    13201 aacctacttt ctcttttctt tctattgatt catcttatcg tctagaatat ccacatcgcc
    13261 tgtctctcca ataaccagta gattatcatt ttcacgaaca tattcatctg ctgcaggaga
    13321 agcgataacc gtttgatcta ctcttcgtat ggcgactact gttaagtcaa aacgttgcct
    13381 gaaatttaat tcagctaatg ttttgttgaa aaatttagga ttggagactc ggatttctgc
    13441 taaagaatat ttatctgaca actctaggta atctaaaata ttctttgaca ctaaattatg
    13501 cgcaatcctt attcccatgt ctcgctcggg atggacgacg cgatcagctc cgatcttttc
    13561 aagtactctg gcatgatact cattttgggc ttttgctaaa atatttggta cgcccatttc
    13621 ctttaccatc agagtaacta agatactggc ttgaatatct tctccaatag cgacaattac
    13681 atgatcgaaa tttcttatgc ctaatgatcg aagtgtcatt tcatcctgtg catttgccac
    13741 aacagcatga gtggctatat tcatatattc attgacccga tcttcattac tatcaatagc
    13801 cagtacttct tgaccagctt cgatcagtgt tcggcagata cttccgccaa atcgtccaag
    13861 acctacaatt gcaaaatttt gtttcaccgt tttcctcctt tagtatcttc ttttctcagt
    13921 ataacaaagc ctttacaaaa acaaaagcaa gcatcattat tgtaaaaaag ccaagaaaaa
    13981 taatagttat tttctcagct tttgattatc ctctttatca tcgtttttat ttcaccaaac
    14041 catgtttgaa tgcatagata gcggcttgtg tacggtcttc cacttctagc ttagccaata
    14101 tattggaaac atgtgttttt actgttttca gtgtaataaa caattcgtcc gctatttcct
    14161 gattactttt tccttcagaa atcagcatta aaatttcatt ttcccgattc gttagttctt
    14221 catgtaatac aggctcattt cgccgactca ttttttccat catttttgtt gtcacttctg
    14281 gttctaatac tcgctcgcca cgttgcgttg cccttatagc atcagcaatt tcatgtgcgg
    14341 tagaggtctt caacaaatac cctgctgcac ctgcttcaat ggcaggataa actttttcgt
    14401 catcaataaa actggttacg ataatgattt tagcttctgg ccaatctttt aagattgctt
    14461 ttgttgattc gataccatcc atttcttcca tgactaagtc cattaaaatg acgtccggtc
    14521 gaagtgtcat cgccttctca tagccttgtc gtccattttc agcttctcca attacttcaa
    14581 tgtcttcttg aatagataaa taagaagaga cgcctaagcg caccatttca tggtcatcta
    14641 ctaataaaac ttttatcatc cttatctatg cctccttcat caaagggacc ttgatttcta
    14701 cacttgtacc ttgaccttta aaactaatga tttttactgt tccaccaata ccagctactc
    14761 gttctttgat attgttcaat ccataacttc ccgttttcgt ttcattcata ttaaaccctg
    14821 ttccgtcgtc aataatacgt aacaaaagat tattgtctat tttgtgcaaa tatacctcta
    14881 attcattggc tttagcatgt cttaatgtat ttgataacaa ttcttgaacg attcggaaca
    14941 agtgatcctc aatggaacta gatagtttta catcttctac atcccatttc agcgaaatct
    15001 ggattttatt ttgcaattcc ttcaataact gctcgatacc ttgctttaaa cttttctctt
    15061 ctaaattgac tggacgtaga tgaagaagca gcgcacgcat ctcagactgg gaagcgttga
    15121 tgatctctgc taccattttc aactgcttgc gaaacatttc tggtgtctcg cttttttctg
    15181 cttgctccgt caatgccgac atcatcatca ttgctgcaaa caattgttgc gaaactgaat
    15241 catgcagctc acgggctaat cgatgtcttt ctaattctaa aatttcttct ttggtttgtc
    15301 catctacata ttggggacgg ctcgttacaa tttgtaattc actagatact tcaatcattt
    15361 tttcttttat cttagtgatt tccttatcaa tatctttgat gtataaatcg tccgatgcac
    15421 ttgggatcgg aagaagcaac aatttggatt cataattgcc agaagaaagt gcacgcagct
    15481 tttcttcgat tttcccgtat tttgtctttt gaataagtga taataataga aagacaatta
    15541 atcctacacc taaggaaatg gctaacaaat ggaagataag cggaacatat aataaacgtg
    15601 cacgcagtaa ttctccccac caatgacttt gattgctggc ataaaaataa gtgaaaagtg
    15661 aaaatagaat aataaggaaa gccgcaatcc ccgaataaac agctagcatc gctctggata
    15721 tttttcccat catattcgta tcacctcgac atctccaatc aaggtattag taataatctt
    15781 caatctacga gggttttcat cataatcact gctgtagatt ttgatttgtt catttttcaa
    15841 agaaaattgc tcttcttcaa acaagacatt tcctactaat gtcgcatgct ctaatcggat
    15901 ggctacgccc aaaggtacta agatccgtgt tctgcctatc ccttttctta caatcacaat
    15961 attatcatct tttggcaaaa gcgtattgcc caaatctatg atagtatcac cagaaatgac
    16021 ggcgatgttg atatcgtccc attcatagac gtcgtttcca atacgttggt taccgaataa
    16081 ttgctgtctt ttccgctcat tattatgtgt ttttaattgc tctgtttgga tcatcatgat
    16141 ctgtttcttt cgccaaaaag catttttggt taaatctatc cctgaaatct ctacaccttt
    16201 cagaccaata aataaaacgg caaatacagc catcatccat aaagcaggac tgttgactaa
    16261 gctaaaaaaa atgaccaaac ttcctaatat caattgaaaa ttttgaaatt tagtacgagg
    16321 atgtttgcgc atagctagat atatattgaa aataccgaat atcactaaaa gcaaaagtcc
    16381 agtattgttt acgatttgcc aaacagcaaa taacagcagt agtgcttcaa ctaccacaaa
    16441 aaaacgccaa gaactattca tcgctctttc tccctcctga cacattctca tttcatcata
    16501 cctatttttt taactgttgg ctataggact ttagatgtaa gatcgtttgt tgaaatctaa
    16561 aatacacaaa gagaaaaaga tctagagtgt atcatcagta agatgaactc tctagatctt
    16621 tcagtcagac tacgcttgtt ctactttgac gattttaact aacatatcgc cacctggagt
    16681 agcaatagtt acttcatcat ttttacgttt gccaatcaat gcttgcgcaa taggagaatc
    16741 attggaaatt ttgccagaaa acggatctgc ttcagcgctt ccgacaatcg tgtactcttc
    16801 ttcttctcca tctggtagtt cgatgaatgt aactgtttta ccaattgata cctcatctgc
    16861 atctacaccg tcgttatcga tgatttgagc aaaacgaatc atattttcaa ttgttgtgat
    16921 tcttccttca acaaatgctt gttcatcttt cgctgattca tattcagagt tttcggaaag
    16981 atctccgaaa ccgcgggcaa ttttgattcg ttcaatgatt tctccgcgtt tgactgtttt
    17041 tagttcttct aattcttttt ctaatttttc tttaccttct agagtcattg gataaacttt
    17101 ctctaccatg tgtaacgtct ccttttttcg ataaaatacc gcaaaaggta atcttttgag
    17161 gaatatagtc caaaaaagat taccacgctt ttcttcaaat gtaaatgttt ttctaaaaat
    17221 gatcaatgtc tagagcctgt ttttctaaga attatttaca tatttttcca ctaattccat
    17281 atgttcttca taagttttag cgaaataaac atttccagtg ctcgtatctg ctacgaaata
    17341 ataatagtca ttttctgctg gttctaatac tgccttgatt gcctcttcgc ttggattgtc
    17401 aaaaggtcca ggtccatagc cggtattggt atacaaatta tatggtgaat ctacagctgt
    17461 atcttcatac gtcactaatt ctttgtgttc acctaatgcg tataaaacag agatatctga
    17521 ctgaagtggc atatttgctt tgattcggtt aaagaagact tgtgcaatgt ttttacgatc
    17581 attttctttt acgccttctt tttcaactag agaagctaga gttaaaactt cttgtacagt
    17641 caaatttttc tgcttgatag ctgaataata gttttgcatg accgtattcg tcttgttcac
    17701 catttcaatc actagatcct taagcgaagt actcgtgtaa taatcatatg tggctgggaa
    17761 taagtaacct tccaaacggt attttgtgtt ctcagcttta gatgcacttt ccaacagttc
    17821 tgggaatttt tggtgcaact cgttaaagaa agtctcatca ttcactaaat ctaaaaattc
    17881 tttcttgtct ttaccagtta ccttcgctac ccgttcagca atctgatcga tatcgtaccc
    17941 ttctggaatg gcaattttcc catctgcaac ttttgttggc tcagaagtac cgccttcttg
    18001 caactgtttg ccaatctcgt caagagtcat attaggcgct aactgataat agccagcttg
    18061 aaagccagtt aaatttttaa attttgtata ataattgaaa acaatcccgc ttttaataat
    18121 attgtccttc tccaaaatct caccaatctg cttgttagaa gagccactcg ggatctcgac
    18181 ttggactaat tggtcatctg atttatctaa aggcttcaac ccgctatcta cataacgata
    18241 cacggtaaat ccaagaaatc cgccaataat caataatgta agagcaatca ctaaaatgat
    18301 cttcctcaca atcttatctt cttttttacg cagatttctc cgcttgtcga ttatatctgc
    18361 tgtagctgcc ttactggctt cttttttatc tttacttggg tgttgtttgt ttcctgacaa
    18421 aacaactcct ccttactatc aagttcgtaa ctttattcat tatacatgat atttgaattg
    18481 gcgtgaatga ttttatttaa atattctatc aaaaatgtaa gtagtaaatc cattactata
    18541 agattaaaat aaatgttatc aagatacaag ctattttagc tttattcttt ctgtaaaaaa
    18601 gaaaggagac taaataaact attatgaaaa aaggaaaaca tgctaaaaaa agaaaatatt
    18661 catttttaat gatcgttgtc gtgttgttta gccttagtgc gttctctaca ggaactattc
    18721 ttggatggca attttacaat aatgaacact ctcagatagt tgcccatgct caatcagctc
    18781 aaaaaaagtt gatactgact accaatgaac aacagctcgt tgaaaaaatc gatcaactat
    18841 taagaaaaaa tgattatatt ggctcaattt acgtaaagaa aaataatcgc atcatcatgg
    18901 aaaaaggcta tggctatgcg aataaactca cgcaacaacc aaatgatcct tctctttatt
    18961 atcagattgg ttcggttcaa aaagcattga ctactctttt gattttaaaa caagtcgagc
    19021 gtgggcacct ttctttagat actaagctgg ctcaattcta tccacaaatt gctggcagcc
    19081 agcaaattac tatccgtgac ttgctgtata tgcgctcagg cttgcaccga actgcttctc
    19141 caaccatacc gatgactgat gaagaagtcg ttcaatttgc gattcagcac ttaaaattta
    19201 cagattatca gacctctcat tatgattcat tgaactttac catattgact ggaattttga
    19261 tcaaattaac aaatcaaccg tatgaaaaat tattgaccga tgatatcatc gaacctttag
    19321 gattacagca tacaaaattt tatgaccaag tcaaaaattc atccaatcat gcactatcct
    19381 atcaaatgtc taaagaggat gattacttta aaccactgac agaatctgaa acagagatcc
    19441 ataacgaatt agggactggt aatatcagta tgtccgtcta tgatttaaat acgctgttta
    19501 cacaagtttt atctggaaaa ctagtttcca aagacttact cttctcatta tggcgtgaaa
    19561 atgcgaatgg acatccatat agtggtgggg tatatagcgg agataattat atcctgtcgc
    19621 aaggaaatat cgactctttt gatacatctg ctatatttag caaagattca caggatgcta
    19681 tcatcatgga aagcaatata aaagctgaca aaactatcaa actgcctact gctgatctaa
    19741 gaaaccaaat ctatgacctg atggaaggaa ctactaagtt ggcaaatagc tgatatgatg
    19801 aaaagatagc aaatactact tctttgaata gcatttgctt aaaaaaagta ctcgactaaa
    19861 cgtaggaaag agtgtgggac aaatctaaaa atcaattttt gctacagact ttaattccaa
    19921 ataaacagaa cagaaagagg tgttcttctt tctgttctgt ccaaattatt ctaagatttc
    19981 tctttaccaa cattatttga caaagggcct attaaatcta aaatagtatc tttagattgc
    20041 taaaatatgt gctagcaaat cattcatggc ttgttcttat ttttaatttt atgcaataaa
    20101 aaagaagaac aattagcgtg attatagaaa taaataatga gattttagaa aaaaatgtgt
    20161 atgaatacct gattgttact ttatttattc cactttcact ttttactgta acaaaaggac
    20221 ctccttgttt tagtactgta gcattttttg aattaacagt aaccgttaat cctttatacc
    20281 ggaatacagg taaaactatt gttttgcctt ttgaagattt aaacgtaaat gtaatttcat
    20341 ctttatttgg agaaaagcta ctataaataa cttgaccatc ttctgtaact tgatggtttt
    20401 tcaaaagcaa ttgatctttt ttatagccta taggtacata atcaaaattc tcaaattctt
    20461 caagtttatt ttccatttgt tcattagaat aagttccttg aaaattcatt tctttcacca
    20521 tgttaactac tgatgaataa tttaaaattg aaatagctaa tattaataat agtataaaat
    20581 ttcttctcct tgtataagaa aatttttcca ac
//
LOCUS       DACDUU010000056        20069 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.056, whole genome shotgun sequence.
ACCESSION   DACDUU010000056 DACDUU010000000
VERSION     DACDUU010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.056"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(111..626)
                     /locus_tag="IWL30_001994"
     CDS             complement(111..626)
                     /locus_tag="IWL30_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-keto-6-deoxy-D-glucose epimerase"
                     /protein_id="HAP9706733.1"
                     /translation="MVVVMVKVFDIIKFSDFRGYTAVALDNETLFSIGIEFHIKQINQ
                     GFSHNKYTMRGLHYQSGEHAQGKIVMCLHGEIMNIALDIRQGSSTYGCVYVNTLSESN
                     GKCAYIPRGYAHGYLTMEDNTLVQWCVDNDFNSNAAKCISYESVDIEWPVSRQCFVIS
                     EKDKNVERLSF"
     gene            complement(808..1419)
                     /locus_tag="IWL30_001995"
     CDS             complement(808..1419)
                     /locus_tag="IWL30_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP9706734.1"
                     /translation="MIDIISAITVNVLTALYQPFLFAVLTAYLFMYWYLFIVDNSRDP
                     KNLKDSVLIWLRYFKASSKFRRLFFLAFYFTLILFKTLLNRNMWMNPLSNVIGQWSLY
                     IIDNATGESRLTTECVENTMLFIPFCMLLLWWRDIKCDIIRTIYVGIKYVFLFSLSIE
                     FTQLFFRLGTFQLSDLFYNTLGGLIGALLYWLFYRLNKYIDLK"
     gene            complement(1445..2896)
                     /locus_tag="IWL30_001996"
     CDS             complement(1445..2896)
                     /locus_tag="IWL30_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP9706735.1"
                     /translation="MTSNNLIVKTLSRYKNTPLPVKAALWFMVCGFLQRGVSMITTPI
                     FTRVLSTEQYGLYSTYLSWESLLLMITSLSLFKAMMNLYVKYDNQEHILSTVSGLTLV
                     LTIFWFVIAIFIKDILAEILGLTPVLVVCMFSYFIFQAEIQCWSLHKRYIYDYKTLLV
                     ATLAVTVGSSVVGCLAVLFISPTAEARALMNVLVTGVVGVAVYISVFRRCKDFKDNEV
                     WNFAVVFCVSLMPHYLSEFILNGSDKIMINYLCGSSDVAIYSVAYSIGALINLVTNSV
                     NSTFAPYQYQKIKSGEYDLLSKRANEVLGLVAAMLFALMLFSKEIVLIFGGQKYIDSV
                     NLIVPICIGIYFNYMFQLFARVQEYFEKKLTVVIPSVLCAIMNIVLNYVFIKLYGYQA
                     VAYTTVFSYMFFCVIHYIFYRKVCKELLDGKNIYDVRGILLISAITIAGGILISIINK
                     FDFIKYLIIAIACVAAIIFRKRLLLLVRRIMSK"
     gene            complement(2920..3651)
                     /locus_tag="IWL30_001997"
     CDS             complement(2920..3651)
                     /locus_tag="IWL30_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706736.1"
                     /translation="MEKKMTVIITMAGLGSRFRKIGYAVPKYMINAKGRTLFDWAMDS
                     LVGYNKNVDKYIFVVRQEDNASAFIKEHCAYFGIENVEVIEIDYMTDGQATTCMLAIP
                     YCNQDNEIMVYNIDTYVESNEMKYEDICGDGHIPCFHADGDHWSFAKLNDRNEVIEVR
                     EKVRISDNCTLGAYYFSSAKLYKALYEEYYADSSNMEKNEKYIAPLYNFMIKKGLKVT
                     ISIVDAKKVHVLGTPEELNVFLNQE"
     gene            complement(3669..4064)
                     /locus_tag="IWL30_001998"
     CDS             complement(3669..4064)
                     /locus_tag="IWL30_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAP9706737.1"
                     /translation="MYSEYSFVFDIDGTLCPIKKKDERYEDLVPYENMVEKLRFYKEN
                     GAKITLFTSRNVNSYKGNLGMINANTAKVLLAWLDKWDIPYDEILYGKPWPGHKGFYV
                     DDRTVRPDEFLRCSVDELNEICNSSKKRF"
     gene            complement(4166..5803)
                     /locus_tag="IWL30_001999"
     CDS             complement(4166..5803)
                     /locus_tag="IWL30_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAP9706738.1"
                     /translation="MKVLIPAAKVVEKELQSIGMLPAIIYPINKKIVFDYLYNQYNAI
                     CDSLDIICYEKADKVHRRLKNYHDSRIHICDLPELGDLGQTVYFGLSDEDEPVLINFA
                     DTIVMESMSDIEMDTFYYSEDFVSSTWTYFKINEGIITDIHDKEEAYVSDKREKLFIG
                     VFMFSSSKLLKSCLREAVESRKQGGINSFYRALQLYSERCPLKAVRTEKWFDIGHIDK
                     YYNTKLEVKAREFNHISIDKDRGILKKTSDDKDKFIGEIKWYLKLPADIEYVRPRIFD
                     YSTSYVAPYVDMEYYAYHTVHELYLYGDLKYNQWHDLFSRIKFICSDFKRYTLKDEKI
                     RESLEDMYLRKTMQRFQKLSEDKRFSAFFEKQIQINGRKFISLTGVIELLKNLIPSML
                     YDVNEFCIIHGDLCFANMMVDSNCSFVKVIDPRGKFGKYDIYGDSRYELAKLFHSLDG
                     KYDYIIKDLFTVEYDTECAVIDYTINTRVRDYDLFELFKSVFSDEIKDDFDKIVLIEA
                     LLFLSMIPLHGESFDHQIVMLATGMELLARVSNKVIL"
     gene            complement(5838..7172)
                     /gene="gmhB"
                     /locus_tag="IWL30_002000"
     CDS             complement(5838..7172)
                     /gene="gmhB"
                     /locus_tag="IWL30_002000"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HAP9706739.1"
                     /translation="MKTVIMAGGRGSRISELFPDIPKPMIPIDGIPVLERELCSLRDQ
                     GFNDIILTVSYLHEKIEEHFGDGSKLGIKIKYFIEKTPLGNAGALYFLLDELGDEPFL
                     LLNADAVFDVDFRQMIRFHVENNADVTLFTHPNSHPYDSGLIISDDNKKVLQWLAKED
                     QRPEYYKNRVNAGLHVINPKVLRENPLMEIVNKSIVEGGGLPYKIDLDRMILKPLANT
                     GRMYCYDSPEYVKDMGTPDRYRAVIKDFQKGIIVGKNLSNPQKAIFLDRDGTINKYVG
                     FLRNAEEFELIDGVSEAIKIINSLGFLCIVITNQPVIARGEVTVDELNSIHNKLETML
                     GHAGGFLNGIYYCPHHPHKGYAGEVIELKIDCNCRKPKPGLLLEASKDFNINLSQSFM
                     VGDSESDVIAGKNAGCKTVLITEDITLPANYGQDYSVCSLLEFVKNHLCTMQ"
     gene            complement(7214..8323)
                     /locus_tag="IWL30_002001"
     CDS             complement(7214..8323)
                     /locus_tag="IWL30_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706740.1"
                     /translation="MNKKEILYNILKKYENLPYVFRNMFGHNISKLVSWKLTADCATV
                     EENSEVYAAFQLRSKDMSDVPVMGLVNCCKDVAIVMQGPLCLKDDFSYNTLAYYKKCY
                     PNALIILSTWKDEDIKALKRIEDLGVIVICSEKPEKPGHLNINYQVGNTLPGIVRAKQ
                     LGAKYVCKTRTDQRIYHPNAMAFFCSLLEQYPVNNEDDWKLIQNQRLLLLSMPYGDMF
                     FPYCLSDFLYFGDVDDMINLFSIPSDVREKGAVSRGVSRREISENNLAPEVQLLRSYI
                     SRMGGNEECSIRAYWEFVKNHVITINKNQIDLYWHKYVGRYSNNTIYGTYYIDDSQDA
                     LHCYNFDFINWLNLYTGKYEYKAEYEKYMDFVWEE"
     gene            complement(8348..9583)
                     /locus_tag="IWL30_002002"
     CDS             complement(8348..9583)
                     /locus_tag="IWL30_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706741.1"
                     /translation="MQISKWKIIQFLMVFLMIISSSILKPLGGWLGETMNVRLVTVIL
                     AIVCLLIASLPAFRKNRYLNFCTLLICVVIIAAYIYSDKTIWVKSRESMSLYYVLLAY
                     PLFCTLINQAWNLDSMLNTICGVTFLSYILRTSISLIQKFSGVLLYENIYRECAPENW
                     YRGGFLRINPPCFTIIIIPIALYMIERQVVARRKVKYYLLIASALAYSAVIHGSRSVL
                     VYQIIVLGCYILFKKGSSKRKIAMWVLAGLLVVIFINSTMFSTFVETFTNSTGEYAGS
                     AESRFASVWFFIPKFLQSPLFGTGILTGEEFTFILPGGVRGHLSDIGFFYTITQYGVG
                     ILIFDILFIIKGFKTALLASKVGMTGYQYLAFGITLNVLLTGINIDWFYPIFTPAIPV
                     CLAVIEYIYQECRSVANIE"
     gene            complement(9614..10744)
                     /locus_tag="IWL30_002003"
     CDS             complement(9614..10744)
                     /locus_tag="IWL30_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9706742.1"
                     /translation="MIGYKVSIMKVLMCESQMPENWYSFDLSKELNQYVDLTLLCKRD
                     VPPLDPKIKSKHILYHKSTNKVFSALVFLKSLFLIWKEIRFGDYDIVHLQLMKSLKYE
                     GGLYVLCKRHYKKLIMTVHNVLPHEVREKDRQEHFNIYSNCDGFIVHNRVTEQHLYAE
                     FPELNAKSYVCPHAIYGDVKHISRAYDKDKFEFILFGQIRPYKGIDVLLNAITELPMD
                     VRKKVHFTIAGQQHPKQDSTDYKKMIEDLKVSDCVLYKPERISDEEMDSMFRNADAAI
                     FPYKEIYGSGALLMAYTYCLPTIVSNVPAFMEETEEGKIGLLFENLNSKSLADTIVKY
                     VNLSPAEKSKIEDDIYELVNRKYTWAASAKLTYEAYKQILNE"
     gene            complement(10731..11504)
                     /locus_tag="IWL30_002004"
     CDS             complement(10731..11504)
                     /locus_tag="IWL30_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9706743.1"
                     /translation="MLYFVIPLMSAKAANNWEMVSTLFNRTLWSCYNQTDPDFKIIVA
                     CHEIPKLTHEYDDRVEFIQVSEKDAPIPTNQDEKMIDKGYKTHALAMRLRELGGGFAM
                     MVDADDLVSSHLAEYVNSHPQENGFYVKTGYVYFVGDDYMKVLPKFSSGSACIVKYAV
                     EDLPEAYPEVMTANCDDNPWIIRKRHGGVVPACEEQGRPLKPLPFKAAVYVLGTGDNH
                     SLYGKTTKYQTKMREILEMFIAKRKIKGKLKAEFSVDWL"
     gene            complement(11518..11973)
                     /locus_tag="IWL30_002005"
     CDS             complement(11518..11973)
                     /locus_tag="IWL30_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HAP9706744.1"
                     /translation="MNAYKLYKVERWFYLHKMEFFAKIVKALIYVLHNSSISYQTEIG
                     DNCKFLYGGIGCVIGKDTKIGNHVIIGTNVLTGGRSNKPGMPTIGNNVYIATGAKILG
                     NITIGDNVIVGANAVVINDVEPNCSVGGVPARVLKKDIDVNDYCSLNRY"
     gene            complement(12395..13501)
                     /locus_tag="IWL30_002006"
     CDS             complement(12395..13501)
                     /locus_tag="IWL30_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9706745.1"
                     /translation="MKILIVVTMTNNFGKKGSYNSQEIGLARGLAELGHEVIVYKSVK
                     SDEEVGTEQLSNTAMIHYIKCRYVGSNGFLSTKNFDKNADVLIQFADLQLSVPHVYSW
                     TVKNGIKYIPYNGITRSQSGNKYIKTIIEILAHRNYKIYKKNGCVAKNDRVSEKLVQL
                     GVNKVKLAPVCVDFEQLHKNYEAIDFTTLREKWNIPRNVRVILYVGRFEKDKRPLDMI
                     SLFNKLDEHNYLLMVGTGALLEQTKQQINLLGLQNRVKIIERIPNKDIWELYCLSDVF
                     VNLNYTEIWGMVLLEAMYYRLGIIAVNAPGPQNIILDNRYGMLAKSDDEILTFLKDGR
                     FTNDIIDAAYQRVTTEFRWSKSGEIMLSMLEGDK"
     gene            complement(13519..14280)
                     /locus_tag="IWL30_002007"
     CDS             complement(13519..14280)
                     /locus_tag="IWL30_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HAP9706746.1"
                     /translation="MEKNMYKRKRDFTVCGIVGVCATTKNEFVREVFSMPTSPVSATV
                     DLVGVPAIISAKENPKIAEMYSQATMAAIDGMPIVKIGRRKGFTCERCAAPDIMGMVF
                     EEGVRQGKTHYFYGGKDNEVLNKLRANLERDYPGIKIVGMYSPPFRPLNEEEDSKICE
                     EINNLNPDFLWVGIGAPKQEMWMWEHQKKIHNTVMFGVGAGFNFFAGTLEKAPEWMEK
                     ASLEWLFRLVKEPRRLWRRYILGGIKYIYYTIESK"
     gene            complement(14356..15354)
                     /locus_tag="IWL30_002008"
     CDS             complement(14356..15354)
                     /locus_tag="IWL30_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP9706747.1"
                     /translation="MKKTIMVTGGCGMIGSNLVKRLVKEGCWDVYVADNLWRGKLEYL
                     NDEDGHPVIDLDTHFFNADLTDYEQAKKVIGTTEYVVHLADVVAGIDYVFKNQGELFR
                     INNLINSNVFDCCRKAGKEKIKGVIYVGTVCSFPLTRQNTLNPEPLREEELFPALPES
                     AYGWSKLMGQLEMGYLEKETGIPCCILMLHNVYGTPTDFGERSQVIPALIRKAINNPE
                     EPFNVWGSGEQGRAFIHVNDIVNALVLALDKGWGHGHIQIGPSHCTSIKEIAYKIIEI
                     SGKDIKPFFDTSKPEGDKARCADYSKAKEILDWEPTVSLEEGLRESYNWIEKQIKG"
     gene            complement(15373..16026)
                     /locus_tag="IWL30_002009"
     CDS             complement(15373..16026)
                     /locus_tag="IWL30_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9706748.1"
                     /translation="MLAEKLNKHIDLLIKRYPTLETIKQDIIDGYLIMEECYLNGGKL
                     LVAGNGGSAADSEHIAGELMKRFKTPRPVSKEFADKLVAIDPVRGPELAKNLECGLTA
                     IPLVAHEALSTAYINDVDGLGVFAQQLFGFGRSGDVFLGISTSGNSKNIMSATVVARA
                     LGIKVIGLTGAKGGELATVADVCVKAPEVETYMIQEYHLPIYHCWCLMLEDKFFGNE"
     gene            complement(16036..17046)
                     /locus_tag="IWL30_002010"
     CDS             complement(16036..17046)
                     /locus_tag="IWL30_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HAP9706749.1"
                     /translation="MIITKTPFRMSFFGGGTDMEDYFKENGGAVLSTTFDKYCYVNVR
                     HLPRFFDYSAELSYSRTERVTSIEDIEHPAIRNAMKMLDMHEIRLTYEADLPARSGLG
                     TSSSFAVGMLNAFYALEGKYADKKKLADEAIYLERVLCNEAGGWQDQIAASYGGFNRI
                     NFNVDGYEVLPVIISPERKKQLNNNLMMFFTGFTRFSSDVQKANNVSGTEDKRVRLKK
                     MYELVDEAEAILTDKNRNLDDFGRQLDVTWRLKKGTGGAISTGSIDELYEKGMAAGAL
                     GGKLLGAGGGGFLVFYVQPEKQDAVRWAMRDLMYIPFEFENGGTRVIHYTPETYIEKQ
                     NG"
     gene            complement(17065..18315)
                     /locus_tag="IWL30_002011"
     CDS             complement(17065..18315)
                     /locus_tag="IWL30_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAP9706750.1"
                     /translation="MKKQYNIAVAGTGYVGLSIATLLAQHHKVTAVDIIPEKVELINN
                     RKSPIQDDYIEKYLAEKELNLTATLDAKAAYQSADFVVIAAPTNYNSRTQHFDTSAVE
                     AVIRLVMEVNPDAIMVIKSTIPVGYTASVRVKTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMDDERLVEDAHTFATLLQEGAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPENLIEAIVESNRTRKDFIADRVLQMAGYYNYAEANQYDAGKEKPVVIGVYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPTLKDGETFFGSKVVNDLEQFKAMCDSVI
                     ANRYDACLDDIKEKVYTRDIFQRD"
     gene            complement(19661..19834)
                     /locus_tag="IWL30_002012"
     CDS             complement(19661..19834)
                     /locus_tag="IWL30_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HAP9706751.1"
                     /translation="MNRYVHGTVQKKNRKINGKLLVNPNGYEWLRAKGSYPVPKYCNY
                     SETLMEWYQLIIT"
BASE COUNT         6423 a         4342 c         2782 g         6522 t
ORIGIN      
        1 ctatatctaa atttagtttt atgtattcct catagaaata accgagcatt tgctcggtta
       61 ttgttcttct tattttccag tatccttctt ttctccacag ctcattttaa tcaaaagctt
      121 aatctttcca catttttgtc tttctcactt ataacgaaac actgccgact gaccggccat
      181 tcaatatcta cgctttcata acttatacat ttggcagcat tagaattgaa atcattatct
      241 acgcaccact gaactagcgt attgtcctcc atagtaagat agccatgagc atatccacgt
      301 gggatatacg cacatttacc attgctttca ctcaatgtgt tgacatacac acatccataa
      361 gtggaagatc cttgtcgaat atcaagtgcg atattcataa tttcaccatg taggcacatc
      421 actatctttc cttgagcatg ctctccactc tgataatgta gccctcgcat agtatattta
      481 ttatgactaa atccttgatt tatttgctta atgtgaaact caataccaat cgagaaaaga
      541 gtctcattat ccaatgcaac agcagtatat cctctgaaat ccgaaaactt aattatatca
      601 aacactttta ccataactac caccatgtta tatattttac caatttttta attcgctcga
      661 cctcatttaa agcaataaaa ttccctacct cttgaagata taacaacaat gttttagcaa
      721 aaatagatgg catgtaaagt attcttgatg agattacaca atcctgtctg cagaaaaata
      781 ttaaccatat taaaattcaa agcagttcta ttttaagtct atatacttat ttaatcggta
      841 gaatagccaa tatagcaaag caccaattaa gccccctagg gtgttataaa acaaatccga
      901 tagttgaaat gtaccaagcc taaaaaacag ttgtgtaaat tctatactta atgagaataa
      961 aaacacatac ttaatcccaa catagatggt gcgtataata tcacatttaa tgtcacgcca
     1021 ccataacaat aacatgcaaa aagggataaa cagcatcgtg ttctccacgc attctgttgt
     1081 caatctgctc tctcctgtag cattatctat aatatataat gaccactgtc caataacgtt
     1141 tgaaagcggg ttcatccaca tatttcgatt aagcaaggtt ttaaataata tcaaagtaaa
     1201 ataaaacgca agaaaaaaga gtctgcgaaa tttactagat gctttaaaat atctcaacca
     1261 gattaataca gaatctttta agtttttagg atctcgagag ttatcaacta taaatagata
     1321 ccagtacata aacagatacg ctgttaatac ggcaaataag aacggctgat ataatgctgt
     1381 taatacattt accgttatag cagaaataat atctatcatt ctctatctct ctatgagcaa
     1441 atgtttattt gctcataatt ctccttacta aaagtagcaa tcgttttcta aatataattg
     1501 cagcgacaca agcaattgct ataattaaat attttatgaa atcaaattta ttaattattg
     1561 aaattaggat gccaccagct atcgtgattg cagaaatcaa aagtattcct ctgacatcat
     1621 atatgttttt tccatccaat agttctttgc aaacttttct ataaaaaata taatggatga
     1681 cgcagaaaaa catatatgag aatacagttg tgtatgctac cgcctgatac ccatataact
     1741 ttataaaaac ataatttagt acaatattca taattgcaca caaaaccgat ggaataacga
     1801 cagtgagttt cttttcaaag tattcctgta ctctagcaaa cagctgaaac atatagttga
     1861 aataaatacc gatacatata ggtacaatta aattaacact gtcgatatat ttctgcccac
     1921 caaatatcaa cacaatttct ttactaaata gcattaatgc aaaaagcata gccgctacca
     1981 aaccaagaac ttcattagct ctttttgata gtaaatcata ttctcctgac ttgatttttt
     2041 gatactgata aggtgcaaaa gtggaattga cagaatttgt aacaaggtta attagagcac
     2101 caatagaata tgccacgctg taaatagcaa catctgacga accacataag taatttatca
     2161 taattttatc agaaccattc agaataaatt cagataaata gtgtggcatc aacgatacac
     2221 aaaatactac tgcgaaattc cacacttcat tatccttaaa atccttacat cttctaaata
     2281 ccgatatata cacagccacg ccaacaacac cagtcactag gacattcatt aaggctctcg
     2341 cctcagcggt aggagatata aacagaaccg ctaaacagcc aactacagat gagccaacag
     2401 taactgcaag ggtagctact aataaagtct tataatcata tatatatcgt ttgtgtaaag
     2461 accaacattg tatttctgcc tgaaatataa aatatgagaa catacagaca actagaactg
     2521 gtgtcaatcc taaaatttct gccaatatat ccttaataaa aatagcaata acaaaccaaa
     2581 aaatagtcag tactaaagtc aatccggata ctgtcgaaag aatatgttct tgattatcat
     2641 attttacata aagattcatc atggctttga acaaactaag cgatgtaatc atgagcaata
     2701 aggattccca cgatagataa gtgctgtata aaccgtattg ttctgtagac aaaactctag
     2761 taaaaattgg ggttgtaatc attgaaaccc ctctctgtaa aaaaccacat accataaacc
     2821 ataatgcagc cttaacagga agtggagtat tcttatatct gcttaatgtt tttacaatta
     2881 gattattact ggtcactgct attttctcct aaacactatt tattcttgat tcaaaaacac
     2941 attgagctct tctggagtac caagcacatg aacctttttc gcatctacta tgctaatagt
     3001 cacctttaac ccttttttta tcataaagtt atataatggt gctatatatt tttcattctt
     3061 ctccatatta gaggagtctg cataatactc ttcatataaa gctttataca gctttgccga
     3121 tgagaaataa tacgcaccaa gagtacaatt atcagaaatg cgaacttttt cgcggacttc
     3181 tattacttca tttcgatcgt taagctttgc aaaactccaa tgatcgccat cagcatgaaa
     3241 gcatggaata tgtccatctc cacaaatatc ttcgtatttc atctcatttg attccacgta
     3301 tgtatcaata ttgtaaacca taatctcatt gtcttgattg caataaggaa tagccaacat
     3361 acatgtagtt gcttgaccat ctgtcatata gtctatttca atcacttcta cgttttcaat
     3421 accaaagtaa gcacaatgct cctttataaa agctgaagcg ttgtcttcct gacgaacaac
     3481 aaaaatatac ttatcaacat ttttattgta tccaaccaaa ctatccatgg cccaatcaaa
     3541 taaagttctt cctttggcat taatcatata tttaggaacc gcataaccaa tttttcgaaa
     3601 acgacttccc aaacctgcca tcgtaatgat aactgtcatc tttttttcca tagaactaac
     3661 ctccttattt agaatctttt ttttgaactg ttgcagattt catttaattc atctacagag
     3721 catctcaaaa actcatcagg tctaaccgtt cgatcatcta catagaagcc tttatgcccc
     3781 ggccaaggct taccatacag gatttcatca tacggaatgt cccatttatc caaccacgcc
     3841 aagagcactt ttgcagtatt agcatttatc attcccaaat ttcctttata ggaattaaca
     3901 ttgcgtgagg tgaacaatgt aattttagca ccattctcct tatagaatct cagtttttca
     3961 accatattct catacggcac caaatcctca tatcgctcat ccttcttctt tatcggacac
     4021 aaagtgccat cgatatcaaa cacaaaagaa tattcactat acatttttct cttcccttct
     4081 attgtattaa ttaatataat cgtgccttaa tgcctacatt gctaaaacag tattgtaacc
     4141 gttattcatt aaggcacaat ttatattaga gtataacttt attacttact cttgcaagaa
     4201 gttccatacc tgtagcaagc atcactattt ggtgatcaaa actttcaccg tgtaatggaa
     4261 tcatactaag aaatagcaac gcttctatca atacaatctt atcaaaatcg tccttaatct
     4321 cgtccgaaaa gaccgactta aacaattcaa ataaatcgta atctcttaca cgcgtattaa
     4381 ttgtataatc tatcactgca cactcggtat catattctac agtaaataga tccttgatga
     4441 tataatcata tttaccatct aaactatgaa atagttttgc taattcatat ctgctgtccc
     4501 cataaatatc atattttcca aattttcccc taggatcaat aaccttaaca aaggaacaat
     4561 tggaatctac catcatatta gcaaaacaca aatctccatg tataatacaa aattcattta
     4621 catcatagag catacttggg ataagattct ttagaagttc aattacaccg gttaaagaaa
     4681 taaacttacg accattaatt tgaatttgtt tctcaaaaaa agctgaaaat cttttatctt
     4741 cagacaattt ttgaaatctc tgcatggttt ttcgcaaata catatcttcc aatgactctc
     4801 taatcttttc atcttttaag gtgtatcgct taaaatcgct acagataaac tttatacggc
     4861 taaacagatc atgccactgg ttatatttta aatcaccata taagtataac tcatgaaccg
     4921 tatgatatgc ataatattcc atatcaacat agggagctac gtaagatgta gaataatcaa
     4981 aaattctagg tcttacatat tcaatatcag caggaagttt taaataccac tttatttctc
     5041 caataaattt atccttgtca tcgctagttt tcttcaatat ccctcgatct ttatctatag
     5101 agatatgatt aaactctcgt gctttaactt ccaactttgt attgtaatat ttatcgatgt
     5161 gtccaatatc aaaccacttt tctgttctta cggcctttag cggacaacgt tcgctgtata
     5221 gctgtaaagc ccgataaaat gagtttattc ccccctgttt ccgactctca actgcctctc
     5281 ttaagcaact cttcagaagt tttgacgaag aaaacataaa tacgccaata aataattttt
     5341 cacgtttatc ggatacgtaa gcttcctctt tatcgtggat atcagtaatt attccttcat
     5401 taattttaaa gtatgtccaa gtactcgaca caaaatcttc agaataatag aatgtatcca
     5461 tttctatatc agacatactc tccataacaa ttgtatctgc gaagttaatt agaacaggct
     5521 catcttcatc tgataaccca aaatacacgg tttgccctag atcccctaat tcaggaaggt
     5581 cacatatatg aattctggaa tcatgatagt ttttcagtct tctatgaacc ttatccgctt
     5641 tttcataaca gataatatcc aatgaatcgc agattgcatt atactgatta tacaaataat
     5701 caaagacaat ttttttatta attggataga taattgctgg taacattcct atactctgca
     5761 attctttttc aacaactttt gctgctggaa ttaatacctt catctaatta ctccttttac
     5821 aagtttcact aaaccaatta ttgcatagtg cataaatgat ttttaacaaa ttctaacaat
     5881 gaacagacac tatagtcttg gccataatta gcaggcaagg tgatgtcttc tgtaatcagt
     5941 actgtcttac aaccagcatt ttttccagca attacatcac tttcactatc tccaaccata
     6001 aatgattgac ttaggtttat gttaaaatcc tttgatgctt ctaataacag tccaggtttt
     6061 ggcttgcgac aattgcaatc aatttttaat tctataacct ctcctgcata tcctttatgg
     6121 ggatggtgcg gacagtaata aataccgttt aaaaatccac cagcatgccc tagcattgtt
     6181 tcaagcttat tgtgaatact attaagctca tcaacagtaa cttcacctct tgcaattaca
     6241 ggctgatttg ttataacaat acacagaaaa cctagagaat taataatttt aattgcctcc
     6301 gatactccat caattaactc aaattcttct gcatttctca aaaaaccaac gtatttatta
     6361 atcgttccat ctctatctaa aaagattgct ttttgtggat tggatagatt tttaccaaca
     6421 ataattccct tttgaaagtc cttaattaca gctctatatc ggtccggtgt tcccatatct
     6481 ttaacatact ctggagaatc gtagcaatac atacgccctg tgttagccaa tggtttcaga
     6541 atcattctat ctaaatcaat tttgtatggt agccccccac cttcaactat tgatttattt
     6601 acaatttcca ttaatggatt ttctctcaac accttcggat ttataacatg aagacctgca
     6661 tttactcggt ttttataata ttcaggtctt tgatcttcct tcgctaacca ttgaagcact
     6721 ttcttattgt catcggatat aattaatcca ctatcatacg gatgtgagtt aggatgcgta
     6781 aacaaagtaa catctgcgtt attctcaaca tgaaatctaa tcatctgcct gaaatcaaca
     6841 tcaaatactg catcagcatt caaaagtaaa aacggttcat cacccagttc atccaacaaa
     6901 aaatacaatg cacctgcatt gccaagtgga gttttttcaa taaaatattt gattttaatt
     6961 cctaattttg aaccatctcc gaaatgctcc tctatttttt catgcaaata ggaaacagtc
     7021 aaaataatat cattaaagcc ttgatctcta agcgaacaca attctcgttc taaaactgga
     7081 atgccatcta taggaatcat tggcttagga atatccggaa acaattccga aattcggctt
     7141 cctcgtccgc ccgccataat tactgttttc ataatatgcc cttccctaaa gtacttttta
     7201 ctataaaagt taattactct tcccaaacaa agtccatata tttttcatat tctgctttgt
     7261 attcgtattt tccagtgtac aaattcaacc aatttataaa atcaaaatta tagcagtgaa
     7321 gtgcatcttg gctatcatca atataatacg taccataaat agtattattt gaataacgac
     7381 ctacatattt atgccagtac aaatcaattt gatttttgtt aatagtaata acatggtttt
     7441 ttacaaactc ccaatatgcc ctaatagagc actcctcatt gccccccatt cgtgaaatat
     7501 agcttcgaag aagctgaact tctggagcta aattattttc agatatctcc cttcttgaga
     7561 ctcctctaga cacagctccc ttttctctca catcactagg aatcgagaag aggtttatca
     7621 tatcatcaac atcaccaaaa tacaggaagt ctgacaaaca atacgggaaa aacatgtctc
     7681 catacggcat tgatagtaac agtaatcgct gattttgtat taatttccaa tcatcttcgt
     7741 tattaacagg atactgttca agtaaactgc agaaaaacgc cattgcattt ggatggtata
     7801 ttctttgatc tgttctagtt ttacaaacat actttgcacc aagctgcttt gctctgacta
     7861 ttccaggcaa agtgtttcca acttgataat taatatttaa gtgtccaggc ttttcaggtt
     7921 tctcactaca aataactata actccaagat cctctatcct tttcaatgcc ttaatatctt
     7981 cgtccttcca tgtcgaaaga ataattaaag catttggata acatttctta tagtaggcca
     8041 gagtattata agagaaatca tcctttaaac atagtggccc ctgcattaca attgctacat
     8101 ctttgcaaca attaactaaa cccatcactg gaacatcaga catgtccttt gagcgcaatt
     8161 gaaacgctgc ataaacttca gaattttctt ctactgttgc acaatcagca gtcaatttcc
     8221 aagatacgag tttacttata ttatgaccaa acatgttcct aaacacatat ggtaaatttt
     8281 cgtacttctt caatatgtta tataaaatct ctttcttatt catgagatgc actcctatta
     8341 atatttccta ttctatgtta gccacacttc tacattcttg atagatgtat tctataaccg
     8401 ctaagcatac aggaatagct ggagtaaata tcggataaaa ccagtctata ttaatccctg
     8461 ttagcaagac atttaatgta attccaaatg ctaaatattg atagccagtc atacccactt
     8521 ttgatgcaag taatgctgtt ttgaaccctt ttataataaa cagaatatca aatataagta
     8581 tacctacacc atattgagta attgtataga aaaatccaat atcagatagg tgtcctctaa
     8641 ctcctcctgg taatataaac gtaaattcct caccagtaag aataccagta ccgaacaaag
     8701 gcgattgcaa aaatttagga ataaaaaacc aaactgaagc aaatcgagat tcagcagatc
     8761 ctgcatattc acctgtactg tttgtgaaag tttcaacaaa tgttgagaac atggtcgaat
     8821 taataaatat gactacaagc aacccagcta aaacccacat tgcaattttt cttttcgaag
     8881 aacctttctt aaaaaggata tagcaaccaa gtacaataat ttgatagaca aggacagatc
     8941 tagaaccatg aataacagca gaatatgcca gtgcgctagc aatcagtaaa taatatttta
     9001 cttttcttct agctacaact tgtcgctcaa tcatatacaa agcaatcggt attattataa
     9061 ttgtaaaaca tggtggatta attcttaaaa agcctccacg ataccaattt tccggggcac
     9121 actcacgata tatgttttca tagagaagta ctcctgaaaa tttctgaata agtgaaatag
     9181 atgttctaag aatgtatgaa agaaatgtta caccacatat cgtatttagc attgaatcca
     9241 aattccacgc ttggttaatt aaagtacaga acaatggata cgccaataat acatagtata
     9301 aactcatact ttccctagat ttaacccata ttgtcttatc tgagtagatg taagctgcta
     9361 ttataacaac acaaatcagt aaagtgcaga aatttaaata tctatttttt ctaaatgctg
     9421 gtaatgaggc tattaacaaa catactattg ctagaatgac tgtcacaagt ctaacgttca
     9481 tcgtttctcc caaccagccg cccaaaggct tcaatatgct tgatgagata atcattagaa
     9541 agaccattag aaactgtatg attttccatt tactaatctg catgttaatt attattcctt
     9601 tctaattgtt ttactactca ttcaaaattt gtttgtatgc ctcgtatgtg agttttgcag
     9661 atgccgccca ggtatacttg cggtttacaa gctcatatat atcatcctct attttgcttt
     9721 tctcagctgg gctaaggttc acatacttta caattgtgtc tgctaggctc ttagaattca
     9781 aattttcgaa aagtaaccct atctttcctt cctcggtttc ttccataaaa gcaggtacat
     9841 tactaacaat agttggtaag caataagtgt atgccataag aagcgctcca cttccataga
     9901 tttctttata tggaaatatt gcggcatctg catttctgaa catactgtcc atttcttcat
     9961 cagaaattct ctctggctta tatagaacac aatctgaaac ctttaaatct tcaatcattt
    10021 ttttatagtc agtactatcc tgtttaggat gttgttgacc tgctatggta aaatgaactt
    10081 tctttcttac atccattgga agttctgtga ttgcattaag cagtacatca attcccttat
    10141 atggacgaat ttgtccaaac aagataaatt caaacttatc tttatcatac gctctagata
    10201 tatgtttaac atctccatag atagcatgtg gacaaacata tgactttgca tttaattctg
    10261 ggaattcagc atataaatgc tgttctgtta ctcgattgtg aacaatgaaa ccatcacaat
    10321 ttgaatatat attaaagtgc tcttggcgat ccttttcacg aacctcatgt ggtaacacat
    10381 tatgaactgt cataattaat tttttatagt gtctcttaca taaaacgtac aatccgcctt
    10441 catatttgag acttttcatc aattgcaaat gaacaatatc ataatcacca aatcttattt
    10501 ccttccaaat taagaacaag cttttcagaa aaacaagtgc cgaaaacact ttatttgtac
    10561 ttttatgata caaaatatgt ttgctcttaa ttttaggatc taaaggagga acatcacgct
    10621 tacacagcaa tgttaaatca acatattgat taagttcttt agaaagatca aacgaatacc
    10681 aattctctgg catttggctt tcacacatca ataccttcat aatactcacc ttataaccaa
    10741 tcaacagaaa attctgcttt cagttttcct ttaattttac gttttgctat aaacatttcc
    10801 agtatctctc gcattttcgt ctgatatttt gtcgttttac cataaagcga gtgattgtcc
    10861 cctgttccta atacatatac tgctgctttg aatggaagag gttttaatgg acgcccttgc
    10921 tcttcacatg ctggaacaac gcccccatgt cgttttctaa tgatccatgg attatcatca
    10981 caatttgctg tcataacttc cggatatgcc tcaggcaggt cttccactgc atattttact
    11041 atacacgcac ttcctgatga gaactttggt agaaccttca tataatcgtc tccaacaaag
    11101 tacacgtatc ccgttttgac ataaaatcca ttttcctggg gatgggaatt cacatattcc
    11161 gccaaatgag aacttacaag atcatctgcg tcaaccatca tagcgaaacc gccgcccaac
    11221 tctcgcaaac gcattgcgag agcatgtgtt ttataaccct tatcaatcat tttttcgtct
    11281 tgatttgtgg gaataggggc atccttttcg ctaacttgaa taaattcaac tctgtcatca
    11341 tattcatgcg tgagtttagg aatttcatgg caagcaacaa tgattttgaa atcaggatca
    11401 gtctgattat aacacgacca taatgttcgg ttaaataagg tactaaccat ctcccaattg
    11461 tttgctgcct tcgcactcat taaaggaata acaaaataca acatacaatc tctcctttta
    11521 gtaacgattc aaactacaat aatcatttac atcaatatct tttttcaata ctctagccgg
    11581 aacgcctcct accgaacaat taggttcaac atcattaatg accacagcat tagcccctac
    11641 tattacattg tcccctattg tgatattacc taaaattttt gcgccagtag cgatatatac
    11701 attgtttcca attgtaggca taccaggctt attggatcta ccaccagtca gtacattcgt
    11761 tccgattata acgtgattac ctatttttgt atcttttcca attacacaac ctatgccacc
    11821 gtataagaac ttacaattat ctccaatttc tgtttgatat gaaattgagc tattatgaag
    11881 tacataaatt agtgccttta caatcttggc aaaaaactcc attttgtgta agtaaaacca
    11941 tcgttctact ttgtataatt tatatgcatt catgttttat ctccccatct ttttagcagg
    12001 taccccccac acttcgtagg gtggaataga cctcaatacc actgtatttg cagcaactac
    12061 agcaccttcc ccaacgcata attctgtgtt agatttacaa agaattttag cacccacaca
    12121 taaaacagcg ttctttttta ctcgaatgat gatacgttca ttactatctg ctcaaccaca
    12181 cgtcacattc tgatagactt taacaccatc ttcaaggatt gtgtcagaat gaatcacagt
    12241 gcctacagaa ttatgaacaa attcaacaga gttccctatc tgtgcacctt taccaataca
    12301 acaaccaaga attttggcta tttttctcgt gcaattatca actatgttta aagcatatcg
    12361 attcctcaat gccattaatt tcgttacttc catatcattt atcaccttcc aacatgctaa
    12421 gcataatttc tcccgacttt gaccatctaa attctgtagt tacacgctga tatgctgcat
    12481 ctatgatatc gttggtaaat cgtccgtctt ttaagaatgt caaaatttca tcgtcagatt
    12541 tagccaacat tccatatcta ttatccaaaa taatattctg tgggccaggt gcattaacag
    12601 caataatccc gaggcgatag tacattgcct ctagcaaaac cataccccaa atttcagtat
    12661 agtttagatt tacaaataca tccgataagc aatataactc ccaaatatct ttgttaggaa
    12721 tacgctcaat tatttttact ctattctgca gtccaagtaa atttatctgc tgcttcgttt
    12781 gctcgagtaa agcaccagtt cctaccatca gcaaataatt atgctcatca agtttattaa
    12841 ataaactaat catatctaac ggtcttttat ccttttcaaa ccgtcctaca tataaaataa
    12901 cacgaacatt gcgaggaata ttccattttt ctctgagagt agtaaaatct attgcttcgt
    12961 aattcttatg taactgttca aaatccacac aaacaggagc aagtttcact ttatttactc
    13021 ctaattgtac taatttttca gaaactcgat catttttggc aacacaccca tttttcttat
    13081 aaattttata atttcgatgc gccaatattt caataattgt ctttatatat ttattgccac
    13141 tttgacttcg cgttatccca ttataaggta tatattttat tccatttttt acagtccatg
    13201 agtatacatg aggtactgat agttgaaggt ccgcaaattg tattagaaca tccgcattct
    13261 tatcaaaatt ctttgttgat aagaatccat ttgatcctac ataacgacac ttgatatagt
    13321 gaatcatcgc cgtattgctt aattgctctg ttccaacttc ctcatcactt tttacacttt
    13381 tatatacaat aacttcgtgg cctaattcag ctaaccctcg agctaatcct atttcctgag
    13441 aattatagga ccccttctta ccaaagttat tcgtcattgt aactactata agtattttca
    13501 tctattttta ttgcccccct acttagattc aatagtatag tagatatact ttatacctcc
    13561 caagatatac cgcctccata gtctacgagg ttcctttacc agtctaaata accactctag
    13621 actagctttt tccatccatt caggagcttt ttccaaagtg cccgcaaaaa agttaaagcc
    13681 ggcgcctaca ccgaacatta ctgtgttatg gatcttcttc tggtgttccc acatccacat
    13741 ttcttgctta ggcgcaccaa taccaaccca cagaaaatct ggatttaagt tgttgatttc
    13801 ctcgcaaatc ttgctatcct cttcttcatt taacggacga aatggtgggg aatacattcc
    13861 aacaattttg attcccgggt aatctctttc taggtttgca cgaagcttat ttaacacttc
    13921 gttatctttt ccaccataga aataatgtgt ttttccctgt ctaacgcctt cttcaaaaac
    13981 cattcccata atatctggtg cagcacatcg ttcacaggta aatcctttgc gccgtccaat
    14041 tttaacaata ggcataccat ctattgcagc cattgtagcc tggctataca tttcagcaat
    14101 tttgggattt tcctttgccg aaataatggc cggcactccc acgagatcaa cagtcgcact
    14161 taccggagat gttggcatgg aaaaaacttc ccttacaaac tcgttctttg tagtagcaca
    14221 aacacctact attccacaca cagtgaaatc acgttttctt ttatacatat tcttttccat
    14281 ctcagtacat ccttactcat atgttcttcc tccgcatcct ctaattacac ggaggaagaa
    14341 cagcttatat acttattaac ccttaatctg cttttcaatc caattgtagg attcacgaag
    14401 accctcttcc aaagaaacag taggctccca atccaaaatc tctttcgcct tagaataatc
    14461 tgcgcaacga gccttatcac cttcaggctt agaagtatca aagaaaggct tgatgtcttt
    14521 gccagaaatt tcaatgatct tatatgcaat ttccttgatg gaagtacagt gagaagggcc
    14581 aatctgaata tgaccatgac cccaaccctt atccagggcg agcaccaatg catttacgat
    14641 atcattgaca tgaatgaaag cacgtccctg ctctccgcta ccccacacat taaaaggctc
    14701 ttcaggatta ttgattgctt tacgaatcag agcaggaata acctgactac gttcgccaaa
    14761 atcagtaggt gtgccataaa cattatggag catcaggata cagcacggaa ttccggtttc
    14821 tttttccaaa taccccatct ccaactggcc catcagtttg ctccagccat aagcagactc
    14881 aggaagagca gggaacaact cctcttcacg gagaggctca ggattcaaag tattctgtct
    14941 ggtcagaggg aaggagcata cggtaccaac atagataaca cccttgattt tctccttgcc
    15001 agccttacgg caacaatcaa aaacattaga attgatcaga ttattgattc ggaacaattc
    15061 gccctggttc ttaaacacat agtcaatgcc agccacaaca tcagccagat gaactacata
    15121 ctcagtcgta ccaataacct ttttagcctg ctcataatca gtcagatcag cattgaagaa
    15181 atgagtatcc aggtcgatga caggatgccc atcttcgtcg ttcaggtact ccaactttcc
    15241 tctccacaga ttatcagcta cataaacatc ccagcagcct tccttcacca gtctcttaac
    15301 aagattggag ccaatcatac cgcaaccacc ggtaaccata attgtctttt tcatttaaca
    15361 ttcctccaaa ttttattcgt ttccaaagaa tttatcttcc agcatcaaac accaacaatg
    15421 atatatcggc aagtgatact cttgaatcat gtatgtttca acttcaggag ccttaacaca
    15481 aacatctgct accgtggcaa gttccccacc ctttgcgccc gtcaagccaa taacttttat
    15541 accgagagcc ctagcaacaa cagttgcgct cataatattc ttgctatttc cggacgtcga
    15601 aataccaagg aatacgtcac cagatctacc aaagccaaac agctgctgtg cgaagacacc
    15661 caaaccatcg acatcattaa tataagcagt agacaatgct tcgtgtgcga ccaaaggaat
    15721 tgccgttaaa ccacactcga gatttttggc taattccgga cctcttacag ggtcaattgc
    15781 aactaactta tcggcaaatt ccttacttac aggacgtggc gtcttaaacc gcttcatcaa
    15841 ctcgccagca atatgttccg agtcagcggc tgatcctccg tttcctgcca caagaagttt
    15901 accaccattc aaatagcatt cctccataat taggtatcca tcgataatgt cctgtttaat
    15961 cgtctcaagt gtaggatatc gtttaatcaa taagtcaata tgtttattaa gtttttccgc
    16021 taacattgtt actccttatc cattctgttt ttcaatataa gtttccggcg tgtagtgaat
    16081 aactctagtt ccaccatttt caaattcaaa cggaatatac atcaaatcac gcatagccca
    16141 acgaactgca tcctgtttct caggttgtac atagaaaacc aagaaacctc caccacctgc
    16201 accgagcaac tttccgccta aagcacctgc agccataccc ttttcatata gttcatcaat
    16261 gcttcctgta ctaattgctc caccagtacc ttttttcaat ctccaagtaa catcaagctg
    16321 tctaccgaaa tcatctaaat ttctgttctt atcagtaaga attgcctcag cttcatctac
    16381 aagctcatac atctttttca gtcttactct cttatcctct gtgccactga cgttgttagc
    16441 cttttgaaca tcagaagaaa atctagtgaa tccggtaaag aacatcatca gattgttgtt
    16501 aagctgcttt ttacgttcag gcgaaataat gacaggcagc acctcataac catccacatt
    16561 gaaattaatt cgattgaacc caccataaga tgcggcaatc tgatcctgcc atccaccggc
    16621 ttcattacac agaactcttt caagataaat ggcttcatca gcaagtttct tcttgtcagc
    16681 atacttaccc tccagtgcat aaaaagcatt caacatacca actgcaaaag aactggatgt
    16741 acccagacca gagcgagcag gtaaatcagc ttcgtatgta agccgaattt catgcatatc
    16801 gagcattttc atcgcattac gaatcgcagg atgctcaata tcttcaatgc tagttactct
    16861 ctcagtacga gaataagata actcagcgct gtaatcaaaa aatctaggca gatgtctcac
    16921 attcacataa cagtacttat caaaagtagt agaaagaact gcaccgccat tctctttgaa
    16981 ataatcctcc atatccgttc caccgccaaa aaaagacata cgaaacggtg tttttgtaat
    17041 aatcataaat actctctcct aatcttagtc tctctggaag atatcacggg tatatacttt
    17101 ttccttaatg tcatcaaggc aagcatcgta gcgatttgct ataacagaat cacacattgc
    17161 cttaaactgt tccaaatcat tcacaacctt actgccaaag aaagtctctc catctttcaa
    17221 ggtcggctca taaatcacaa cagttgcacc tttagctttt acacgcttca taacgccctg
    17281 gatagaactc tggcggaagt tatcagaatt ggatttcatg gtcaaacggt aaacaccaat
    17341 cacaacaggc ttttctttgc cagcatcata ctgatttgct tctgcgtaat tgtaataacc
    17401 agccatctga agtacacgat ccgcaataaa gtccttacga gtacggttag attcaacgat
    17461 tgcctcgatc agattttcag gaacatctgc gtagttggcc agaagctgct tggtatcctt
    17521 cggcaggcaa tagccaccgt aaccaaagga agggttgttg taatgagtac caatacgagg
    17581 atcaaggcaa actccattaa tgatctgctg agtgttcaag cctttcatct ctgcataagt
    17641 gtccagctca ttgaagtagc tgacacgaag agccagatat gtattggcaa acagcttaac
    17701 agcttctgct tcagtaaagc ccatgaacaa tgtgtccaca ttctctttga ttgcgccttc
    17761 ctgtagcagt gtagcaaaag tgtgtgcatc ctcaaccaaa cgctcatcat ccatatcggt
    17821 acccacaata atacgcgaag gatacagatt gtcatacagt gccttacttt cacgcaggaa
    17881 ctcagggctg aaaataatat tcttgctacc agtctttaca cgaacagaag cggtgtatcc
    17941 aacaggaatg gtggacttaa taaccataat ggcatcagga tttacttcca tgaccagcct
    18001 aataacagcc tcaacagcgg atgtgtcaaa atgttgagtg cggctattgt agttagtagg
    18061 agccgcaata acaacaaaat ccgcagactg gtatgctgcc tttgcatcca atgttgcggt
    18121 cagattcagt tctttctcag ccaagtactt ttcaatataa tcatcctgga ttggagattt
    18181 acggttatta atcagttcaa ccttctctgg aatgatatcc accgctgtaa ccttatggtg
    18241 ctgtgccaac agagtggcaa tactcaaacc aacataaccg gtgccggcga ctgcaatgtt
    18301 atactgtttc ttcataatat ttcctctttc caccactgct gtggtcacta ctttcttaac
    18361 aaaatgctgt ttgtgaacag taacctgcta tttatataaa acagcccgaa agctgtgttc
    18421 attcatactt aaaacagcca gctcctacgc cggcaaattc aatttttcat aaatccccta
    18481 cccgtttagg gttatattgc aagtttgagt catttttatt gacctcgttt ggggttataa
    18541 tacattttcg cattgaaatg tacctacatt ttccactcaa ccagtcacca aaaagtttat
    18601 aaattcactt attaccctct attcttactc caaaggtatg tttaacatcg ctttattgac
    18661 ctattttcac cccaaatgga agtttgtttg gtacattatt ggtctattcc caccccaaac
    18721 tgtctatttt gagcgagttt ttgtttttta tcattgcaca ttttcaaggc tcaaattcta
    18781 agcggattta ctggcaggga gacattggta aatacttgaa caatttcacg gctttcatca
    18841 tcacatccta cgataataat tctaattgga taaagattat cattaataat ctactttcac
    18901 gtaaaaattc tgaactaaat aaaatctatt gataatattt ttttcttcca ctttttgtat
    18961 atcctacagg tatagtggat ttaattatcc ccaatgcctc tgaagtaaca gacatcacta
    19021 attcaataac acattcacta ccatagtttg taggaacaaa aatgacaaca aaatctgctt
    19081 ccttatgtga tgtttttcca tctgtccaaa ttttattacc aatgaaatct agtttaaaat
    19141 ccctcttaga aactagttca ctctatattt ttattcagtt tcattagttc tgtttttttg
    19201 tcttcagtca ccatatacat atatatctta attatctatc catttcatcc tacgcattct
    19261 tgcgtaaata caattcactc tgttgagctg atataaatca ttggtggtac aaagataaaa
    19321 tgataactgt ttctatacct ccaaaaattc cagtaaatat atctatagat agtttcaaaa
    19381 aaaattgtct tagtttcata atatttaaga tagtggtaca tactttataa caacgtactt
    19441 ttccaaatat tattttccgt tatgaacatc caatatatta caaagtttaa aagtttagat
    19501 gaactataat cttcatttct gcacagtgga taataaattc ttatagataa aaaatagcct
    19561 cttctaaagt cacaaatacc ttactcccac attttaagta aacactctgc agcattctct
    19621 ttgttataaa atgatagata ttcctcaatt ttatctctta ttatgttatt atcagttgat
    19681 accattccat taatgtttca gaataattac aatattttgg gaccggataa ctcccttttg
    19741 ctcgtaacca ctcatatccg ttaggattca ctaaaagttt accgtttatt tttcgattct
    19801 ttttttgaac cgtcccatga acataccgat tcaacaagct aaattataaa aattggtgtg
    19861 ctgtcattat tttttggaaa atttattaat ggctatcaat atacttttgt atcatactaa
    19921 atagctcgtc agttcctata tcggatatac caatattaaa gcatttagat cattatattt
    19981 aagagtggta tcatcccaaa atttcgaata ttttctttta tataagaata atgatactga
    20041 attgccccat tcacctttct ttttttaga
//
LOCUS       DACDUU010000057        19559 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.057, whole genome shotgun sequence.
ACCESSION   DACDUU010000057 DACDUU010000000
VERSION     DACDUU010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.057"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            6..845
                     /locus_tag="IWL30_002013"
     CDS             6..845
                     /locus_tag="IWL30_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP9706752.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            1041..2015
                     /gene="sgrA"
                     /locus_tag="IWL30_002014"
     CDS             1041..2015
                     /gene="sgrA"
                     /locus_tag="IWL30_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAP9706753.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            2313..2669
                     /locus_tag="IWL30_002015"
     CDS             2313..2669
                     /locus_tag="IWL30_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706754.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            2672..3829
                     /locus_tag="IWL30_002016"
     CDS             2672..3829
                     /locus_tag="IWL30_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706755.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(3855..4295)
                     /locus_tag="IWL30_002017"
     CDS             complement(3855..4295)
                     /locus_tag="IWL30_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP9706756.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            4455..4961
                     /locus_tag="IWL30_002018"
     CDS             4455..4961
                     /locus_tag="IWL30_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9706757.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            5129..7042
                     /locus_tag="IWL30_002019"
     CDS             5129..7042
                     /locus_tag="IWL30_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP9706758.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            7047..8048
                     /locus_tag="IWL30_002020"
     CDS             7047..8048
                     /locus_tag="IWL30_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9706759.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(8250..8456)
                     /locus_tag="IWL30_002021"
     CDS             complement(8250..8456)
                     /locus_tag="IWL30_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAP9706760.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(9102..9950)
                     /locus_tag="IWL30_002022"
     CDS             complement(9102..9950)
                     /locus_tag="IWL30_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9706761.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            10114..11016
                     /gene="gnd"
                     /locus_tag="IWL30_002023"
     CDS             10114..11016
                     /gene="gnd"
                     /locus_tag="IWL30_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAP9706762.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            11018..12019
                     /locus_tag="IWL30_002024"
     CDS             11018..12019
                     /locus_tag="IWL30_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP9706763.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            12016..12300
                     /locus_tag="IWL30_002025"
     CDS             12016..12300
                     /locus_tag="IWL30_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9706764.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            12333..13130
                     /locus_tag="IWL30_002026"
     CDS             12333..13130
                     /locus_tag="IWL30_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9706765.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            13144..13977
                     /locus_tag="IWL30_002027"
     CDS             13144..13977
                     /locus_tag="IWL30_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9706766.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            13995..14852
                     /locus_tag="IWL30_002028"
     CDS             13995..14852
                     /locus_tag="IWL30_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9706767.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            14874..15383
                     /locus_tag="IWL30_002029"
     CDS             14874..15383
                     /locus_tag="IWL30_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9706768.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            15420..15821
                     /locus_tag="IWL30_002030"
     CDS             15420..15821
                     /locus_tag="IWL30_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706769.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            15933..16286
                     /locus_tag="IWL30_002031"
     CDS             15933..16286
                     /locus_tag="IWL30_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706770.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            16320..16763
                     /locus_tag="IWL30_002032"
     CDS             16320..16763
                     /locus_tag="IWL30_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP9706771.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            16780..17865
                     /gene="uxuA"
                     /locus_tag="IWL30_002033"
     CDS             16780..17865
                     /gene="uxuA"
                     /locus_tag="IWL30_002033"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP9706772.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            17858..18571
                     /locus_tag="IWL30_002034"
     CDS             17858..18571
                     /locus_tag="IWL30_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9706773.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            19323..19523
                     /locus_tag="IWL30_002035"
     CDS             19323..19523
                     /locus_tag="IWL30_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9706774.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         6766 a         3015 c         4126 g         5652 t
ORIGIN      
        1 gaaagatgat caggattatt cggttgattg gatctgtttt aatagtctta gttggttttg
       61 ttttaggaat tcttgtcaat aatgcacttg cggatatgtt tattggaaat agtgaatgga
      121 ttggagcaat tgccggtacg gtcggacatt tagctgtttt tttactagct ttatttcttg
      181 ctagtaaggt cgaaggaaaa agaatagaag attatggtgt ttctgttcga ggaaaagact
      241 gggggtattt agcgggaggt ctcgttgttg gaattggagt gtttctgctt attactagtc
      301 cattatattt gacgggggcg tatcggttag attcaggaaa cgcaaacatt gtgccgctga
      361 taacgagttt tattttattt attgcagtgg gggcatcaga agagctttta ttccgtggct
      421 tttttcagca tcagatgctt agatttggtc cgttaatagc aatgattgga tctgctgcat
      481 tatttgctct tttgcacgga ttgaatccta acatgacatt tctggcagtt ttcaatatct
      541 ttttagcagg ttgctttttc agcgcactta tctacagtac aggaagttta tttacggcta
      601 ttggtgctca tattacttgg aattggacac aaggagctat tttgggtata ccagtcagcg
      661 gaacgcaaga acaaggtttt ttctccactc tgatccaatc gcaaaataca ataattactg
      721 ggggaaactt tggggctgaa gcatctatat caactacatt attcttgtta gtcttgattg
      781 ttggtttgtt actctatgca tataaaacaa agcggatgaa gaaatacact gctgtttatc
      841 aataaaagat gaagaaagca aggctgatat cggtcttgct tttttaagtc tgtcattgat
      901 taaacgaaaa agtaaacaaa ttcaagcgaa tacacgacat cttttgttag attttcacaa
      961 ctgactgtcg tgttctcatc attttcattt tatataattc ctttatcaaa tagaaggtaa
     1021 ttatatggag agtgatagaa atgaagaaaa cagcgaccgt attatttgtt attcttgggc
     1081 tgagtataat tgcagcaatg attggaatca gtacttctgc aaatgaacgg gcaaatgaga
     1141 atctttcatt tactgtgaaa actgatcgga ttgtttatga catgacacaa caagtcatta
     1201 ctattccagt caaaccaaac aaaagcgtga atgctagtga tgtacatgct gttctaacat
     1261 atggttggga tggtaatgga agttcagaaa aagttattgg ggaagtttat ttaaaagacg
     1321 tacaatggac cgctggtatt gagtatacaa taatgatttc tgcagaacta tcgattgatg
     1381 agatcaagag taaagataaa gttgatttga ttgtttttta tgatggacag atgacgatca
     1441 cggagaattt gaaaccaagc agttggacag ttgttggacc gccaagcagt acggatagca
     1501 caagtagtga aagcagtaca gaaaacacga gcggtgaaag cagtacagag agtacgagca
     1561 gtgaaagtag cacagaaaac acgagcagcg aaagcagtac ggagagtacg agtagtgaaa
     1621 gtagcacagg aagcacgagc agcgaaagca gtacggagag tacgagtagt gaaagtagca
     1681 caggaagcat gagtagtgag agtagcacag gaagcacgag cagcgaaagc agcacaggaa
     1741 aagtaaataa tgaacttgat ttggtaccta tcataccaac taaggaacaa aagagaaatg
     1801 cagggagcaa cctaaaaagt gtgctaaaac agcctgattt tgtttctcgt ggaattgatc
     1861 atcaagaaaa agcgaaagaa acagataata gaaaaagcga tttaccagaa actggtagta
     1921 gaaccttgaa caagctaatt acatggatgg gtgtgttact cattttgatt gtaggagcaa
     1981 gctattttcg ttctctcttc catcgagtga aataaagaat aaaagcaaca gatctaaaat
     2041 tgaataaaac gatagatttt tgagtgaata tttcattcga gagtgctgta ttgcctggcg
     2101 aagttgtagc tttgccagat ctatttttgt ttactaacat atttgagaaa caggaaataa
     2161 caggaacaca aatggaaaat aagagcaaga ataattgaca atgcacactg cgcatggtag
     2221 tatctctgaa aaatcagtaa cgaatctatt tgtacgttat tcatgtagct gtcggaataa
     2281 gtctaaataa tagataaaaa ggagtatgta ctttgtttga agaatttatt gatatcaatg
     2341 aaaggcaagt atatcaattt ttgaactatt gttatgaaag agacgagaaa ttatatgtgg
     2401 tgaaggatat cgctttagat ctaaactaca ccctggctaa gatgaattcg gtgattcaac
     2461 aagcagaatc gttttgcgaa aggtatccag aatataaatt atcttttctt tcagaaaaca
     2521 agatgataaa agtcgagttt tcttctcagt ttttactgag taaagtttat tctatacttt
     2581 tagagggaac gattggttat attttactgg attcacttta caaaggtact tatcaatcct
     2641 tagaaaatct tagtcaaaaa attatataag tttgcgtacg attcaaagaa aattaagaga
     2701 acttaatact attttgaaaa actatcattt atcttgcagc ttgaaaaaag gtaatccgat
     2761 agaaggagaa gaataccgta tccgatactt ttttcacttg atgtattggc agtcattcga
     2821 cgaaaatcat tctgatatat tatcaagtat aaaaaataaa cgtcaactat tagaaaaatt
     2881 tgcgaaacat gcgccatata cgcgtagtat agatagaaag aaatggatca gcctactaag
     2941 aatttcgttg caacgaatga aacaaggcca ctcgattacg tatgtcccag aagagatgaa
     3001 caaatttgtg catccattga taacaaaaga agagtttacg cattacatac tagagccttt
     3061 ttttgaagca aacttctggt cgatgaatca catcactcca acagagcaag cttaccttta
     3121 ttttatgttt ggtgtaatga acacctattt ggaagaagat ttgttggata ctccagataa
     3181 agtacaagaa agttggggct ggattgaatc gtctattgta gaaaattttg aaaaaaactt
     3241 caagatttgt tttacaactt cagaaaaaaa ttatctatat gtcaatttgg caatgatcca
     3301 tttgtattca agggtttttg ggacaaaaaa gaaggtggat gcttttggaa aatcagcaga
     3361 agacagagat tttcagcata tatttccagt gatgtatcca atcatgcaac agttttttag
     3421 tggggtagct cgcagaattc cagagcttaa tacttattat gaaaagaaca gacggttgat
     3481 ttttcaatac tgtatgttag tcagggatat ttttataaaa cacgaacaac ctgtcatatt
     3541 ctatattcaa tcaaagtatg gtaaaacaca ggaattatgg gcaaagaaaa ggatattatc
     3601 tatgactgaa cgcccaataa aatatagtgc atccgctgat cagttacctg atattgtcat
     3661 tagcgattat cctatcgaca aaaaggcata tgatcaaacg aatacacaga tattttactg
     3721 gaatggacaa cctacaaaaa atgattgggc gcgtctgaaa gctgaaatca taaaaatcag
     3781 gaatgagaaa aatagatcca tattggaaaa attggatttc gttaattaag aaaagcagat
     3841 tgtttatgcc aaaattaaga cattgcaaaa agattatttt ctttaaaatc gacaagatga
     3901 ccagaaagca agagttcgtt gagctcttgc tttcttttag ttttagaaag gaacatcctg
     3961 attttatctt cgctaaatcc agtcaaaagt tttgcagatt ggtcgtagat aatcggtttt
     4021 ttcaataaag aaggggatgt acgaatctca atatatagtt cactcaaact gagttcatct
     4081 attctgtgaa caagattttt ataactaata gaatgagtag agagcatttc ggtcacgccg
     4141 ttgtttgtca attttagtaa gtggataaat tcatttttgg ttagtggttg acgaattaag
     4201 ttctgttcga taaacggtat atcatggacg ataagccaac gttttgcgcg tcttgaagac
     4261 gagcagccag aacctgtata caatttaatc atcatacatt cctccaatag ctaagtattt
     4321 atgaaataat aacatgaaga aatagtcgaa aagtgccatt tagaggtgta aaataagaaa
     4381 atgttgtcac gtataatgga actagctaga taagaaatac tattaaatca agaaaaaaga
     4441 aagaaggcac aagtatgtca aacacagccg acgcaatagt agtgatcgat ttacaaaaag
     4501 gggtatgttt tgattcttcg ccgatacacg aattagaaac agtgacagca ttagtgaata
     4561 aaagaatcaa aacatacgaa acgattggga aaccagttat tttcgttcaa cacgaagatg
     4621 ctcaattagt gccacaaaca aaaccatggg agctattgtc ggaattatct attcctgagc
     4681 acagttattt tgtgagaaaa acccatgcga attcatttta taagactaat ttgagtgttt
     4741 tattaactga attggactgt cattcgatcg aattatgtgg ggcacaagca gagtattgta
     4801 tagatacgac tgtgaagttt gctcatggat taggatacca gcttatgatg tgtagaggag
     4861 caacgacaac ttatgataat ccttttatga aagcaaagga aacaatcgct ttttacgaga
     4921 aaatttggga taaacgattt ctgacatttt tggagcaata agttgtttct tcgtttaaaa
     4981 aaagcagaat ggatactggt ttatccattc tgctttatta cgtaaaatga gaaaataatc
     5041 ctgttctatt tttatgggat aaaccattta ttttttgtat aaaaaatgtt tgcgtttaca
     5101 tataattctc ctagaaacat aaaaaggagt gaatagcttg agaacaaaac aagtattaaa
     5161 gggagcgttg gtcatcttat ttgctgtaag tacaagcgtc ccgcaatgga cagtatatgg
     5221 agagagcatt tcttccgaat cggaatctcg ggaggtagaa accacagaaa ctactgatgc
     5281 tggaaatgaa acgaaatcga gtacttcaca aacagaaaca aatactgaaa caacggttga
     5341 gacatctgtt actagcacag atcaagaaac gaccgctacc acagataacc aacaaacaga
     5401 agaagcaaca gctacaaaaa tgtcaaacga cgaatcctct tctgctaaag taagtgacaa
     5461 agagaatggg acagaaaatg atgatatcgg taaaggtgaa aatttcaatg gtaaagaata
     5521 tttgaattac ttcggtaaac agatgacaga aatacttaat ggaccaacag aagacatcaa
     5581 gtcaaaatta atagagttat tagtcactga gcttgctgtt aatgattatg gtgtagataa
     5641 agagattatc aaacatctga ttgctactta tctattgtac gatatcgctt ttgcaaaaaa
     5701 cagtgtgttg atgtcgatgc ttggaggtac agatgatggg acaacgccta cagaaaacac
     5761 aggcttctat ccaaatacaa aacaacttca tcaaactgta ctaaacaaac aagatggcaa
     5821 aatgatggat gtatatgcat attatgtcga tcaaggttca gataaaacag tcatgatcca
     5881 tggcggattt cgagggaatt ggaataacgg gatcgttaca gaagaataca atgattttta
     5941 taaggccggg tataatttat tatttgttga ttccagagcg actggtaata gcggaggcga
     6001 ttatgtgacc tatggacaat atgagtcaga tgacgtcctt tactggataa atcaagaagt
     6061 tcgagagcgg ccatctcaaa agattttact ttatggtggt tcgatgggag cagctacgat
     6121 gatgtctgta ttagcgaaag atattcctgt caatgtcaaa ggaattattg aaaattgcgg
     6181 atttgcatca attgatgaac aactacgttt tacgtacagc cagacagtag cccccgcact
     6241 accggatgcc ataaaaaatc agttagacat cattggggat caagagcatg aagatttatt
     6301 tatgggcctt ttgaaacaat actattttga tcaggagatg catttggata caaaagctgc
     6361 tctacctacg attggaatgt cagattcatt gcctaaattg attatccatg gaacagcaga
     6421 tgatgtagta cctgtgtcta acgcgcaaaa attatatgag ttatcaggag ggtataaaga
     6481 tcttttatta gtagaaggag cagggcatgg aaaggcacag gaagttgacc atgctgccta
     6541 cacgaaacat gtaacggact ttttgaaagt agtgttccat gatcagataa acgtaaagta
     6601 tgtggatgaa aacaataaaa gtctactcaa agaccaggac gaaatccagc tttacggagc
     6661 ttatggagaa aattatatga cagagcaaaa gacattcgaa ggatacgaat tagcaaatgt
     6721 tgaaggaccg actgaaggga tttttaatga gacgatacca actattattt tctattacaa
     6781 aaaaatccca gtagctccac caaaaaagca agatccgaca gagaatgcag acaataaggg
     6841 aaagaatgaa cctcgagagg atcaatcaag tgatgatacg caaccgattc agatgacagt
     6901 gaaagtacaa aaaaagacag atgcaaaaaa aagaatgttg ccgaaaactg gagaaaagaa
     6961 acattcgctt ctcatttatt caggtggatt tttatctgtt agcacgatta tcacatggat
     7021 ttggcgaaga aagagagggt aaatgaatga aatggttcaa gcgaatagtg attgcagtac
     7081 cagttatcat cgtagttatt ttagtcactg tatttttgat taatcaaatc acaccaaagc
     7141 cgattagctt gcttgttaga aaacaatttg acacaagtga aaaagaacaa gtatatgccc
     7201 gaccagatga tttccagcaa aaaatcgaac aaatcgatat aaaaaaagaa atatcctatc
     7261 cttcaaagta caaaaataat aatatggata tctacacacc gaaagtggaa aagaaaaaat
     7321 taccgattct tttctggatg catggaggcg catatgtagg aggggataaa aatgattgtc
     7381 gtgattattt ggaatatcta tgttcagaca cgcagcaaat catcgtaaat atagattatg
     7441 aacgatcacc ggaagcaaaa catcctacac cagttatcca attgaacgaa gccattcaag
     7501 cagctaaaaa agagataaaa gatcaagcag attggtcgaa tatttcgatt ggtggaaatt
     7561 cggcaggtgc acaaattagt ggagaatatt tgttagcact tcaaaatgaa gaaattaaaa
     7621 aagctgatca tcttgatccg actttggaga acaagcagat tacgaagttt atttctttgt
     7681 ctggtctgtt ggatccttct gcacttactc aagtaagtga taaaatctct agctttcttt
     7741 atgaaaaatg tggatgggct tactttgata ataaggactt tgaacaatca gagaaggtca
     7801 aaagtttagc cttggtcaga catgcggatc gttggacgca aaacacattc ttaacggatg
     7861 gaaatacaaa tacgtttacg aaacaaatgg aagaagtaac ttctgtattt gagaaagcgg
     7921 gggtgaaagt cacaaaagta gattatgcgg agaaaaatgc tgttttgaac catgaatatc
     7981 agtttgattt cagcaataaa caagcgcaag aaacatatca aaagttagta atgtttttca
     8041 aggagtaaag tcttatgatg caaaagaggt tgtaatctgt cacttagctc caagtcacaa
     8101 ggagcaattt caataaacaa gcttttcatg gtttatgaat attgtaactt gtgactgagg
     8161 agttagagat ggcaaggggc accttttatt cgaaatttgg gaatatcaca cgattattgt
     8221 cacgatctct tttacgtaaa ttttataagt cagatttgtt gttttgatcg tttaaaaaaa
     8281 gcaacaaatc cgaagcctgc ggaggcaaga agggacaaaa agccgagtgg taagcaaaag
     8341 aatgctggtt ccactaatat tccgttgtta ttgatataag gctgggaaca aagatgaata
     8401 gcaaataaaa tcaatgcact agcgaaaaaa agcaaagaaa tatggatagc tttcataagc
     8461 agcacctcct tttttataat tgtatgataa atagattaag aatctataaa tgtgacaagt
     8521 ttgttaggat gtgaataaaa tgatcatcta gcgaacttga aacaaagatg gctaatgata
     8581 agcaattaat gattcagcaa tcagataggt acaaagttta ccatcatacg tctagaccaa
     8641 taatagaaaa tatgggaata taatagaaaa gatttaggat acccccaaaa gaatttcccc
     8701 aaaaattcga tgtatcctaa acttttttat tttacataac aatcatatca actgtgaaat
     8761 aaaattacaa atgataacct aaaatgaggc caaaagccag tgttatcaag gaaatactta
     8821 attcaccaaa atttttttta attttttaga aaataagaaa caaaaataat aaaaaaaaga
     8881 aaagataaat tttttactac tgtatactaa ctttgttatc gcatctaaca agtaactcga
     8941 ataataccaa tacaaccttc tccaaagatg cgatgacata cgttctaacc caccgactat
     9001 tgatgttctg taccaccctt gcagaaccaa aaaagccact accaaatatt tggtagtggc
     9061 tttttttgtt ttatggattt ttcgcttttt ctgttatggt gctattcttt gatttcagaa
     9121 atagtatgtc gaactttagc gatagaacga cgtgattctt tttcgttgta aaacatcaaa
     9181 atcacatata gagagtccaa aatgcttagt tgagaaatac gggaagctaa agcttcagat
     9241 cggaattctg tttcttcaga aatagaaata aaaacgacat cgccaagttt agcaagggaa
     9301 gaattagctt ggctagtaat aacgattgtt tttgctccag tttttcttac ggtttcagca
     9361 atatggattg tttcttttga ttgccctgtg tgagaaatca aaacagcaca gtctttttcc
     9421 gtcaataaag aagcttccat caattgaata tggtaatccg tgctgtgaga agttgcgata
     9481 ggggaacgca aaaatttatg ataagcgtca gttgctacaa tctctgaacc gccaacaccg
     9541 aaaaaataga cacgtttaga ttcattgata atagtagccg ctttttttag atccttttgt
     9601 tgtaacaact tttttgtgtc atttaaagtt gaaatactag actcgaacac cttttgcgcc
     9661 atagtgagct cagtatctgt tttctgaata ttttcgtgga tagaaatagc agatagatca
     9721 ttttcttgga tcaacatggc cattttaaaa tctttaaatc cattataacc aagttttttt
     9781 gtaaattgaa aaaaagtaga gtcagcaatg cccagttcac ttgctaaatt gctaatagga
     9841 ctatgactta ctttgcttgg attttctaaa atataatcgg cgatagcctg ttcttttggg
     9901 ctgaagtcgt tgtacattgt tttgatttgt agtcttatgg agacgctcat gagaattcct
     9961 cattttcata ataagattat cattttttcc gttctcatta atgttaaaga tacaaggaga
    10021 gtaggaaaaa atcaaacaaa aaatattttt gtaaaatcta tttacaaaaa aatttttttg
    10081 tataatgagg gtataagaaa tggaggcgct atcatggaaa ttggtgtaat tggtttggga
    10141 aaaatgggat tgaatttagc tttaaactta aaagagcacc atcacaaagt acaaggactc
    10201 gacatttcaa gtacagctgt tagtcaggca agagaaaatg acattcctgc ttatcaagag
    10261 atctctgaat ttctagctac ttttgagcag aaaaaaatca tctggttaat gcttcctgct
    10321 ggagagataa ccgaaaacat gttaagaaaa ctttttccaa aactcggacc aggagacatt
    10381 attattgaag gcggaaactc ttattataaa gattcaatcc gtcgagcaga agagtttcag
    10441 aaaaatgaaa tcggttactt ggattgtggg acttctggag gaatcgaagg agcacgacat
    10501 caagcctgct taatgattgg aggagataaa gaaacttttc tatccgtgga acaattattc
    10561 aaagatgtag caattgaaag aggatatctg tatgctggtg catccggaag cggacacttc
    10621 ttgaaaatga tccataatgg aatcgaatat ggaatgatgc aagcaatcgg tgaaggattc
    10681 caacttgtac aaaaaagtga gtatgctttt gacttggaac aagtcgcacg tgtttggaat
    10741 catggttcgg tgattcgcgg ctggctgatg gaaatcatgg aagatcagtt aaacgaacat
    10801 cccaatttaa gtaattatcg gggaatcgtt tctgcatcag gtgaagcaaa atggacggtt
    10861 gaaacagcgc tagaaatggc tgttccagtt ccagtaattg caactagttt gttcatgagg
    10921 aatctttcac aggaagaaga tagtttttca gcaaaagtag tttcagcact aagaaatggg
    10981 tttggcggac atgaaatagt tgaaaagggt gagtaaaatg ccagattatc atatagcaat
    11041 cgttaattcc agcagtttcg gaagagtctt tccacaccac ttggaaagat taaagtcagt
    11101 tggagacgtt catttcttta ctgttgatca agagattcca ggaaaagaac tagcggagtt
    11161 acttaaagga ttcaatatca ttattgcaag tgttacgcca ttttttcgac gagatttttt
    11221 tgaaaacaaa gatgaactgc tattgatttc ccgacatggt attggttaca acaatattga
    11281 tttaaaagca gcaaaagaac accagacaat cgtatctatc atacctgctt tagttgaacg
    11341 aaatgctgta gcagaaaata atataactaa tttgttgtcc atgcttcgac gcacgacaca
    11401 agcacaagca agagtcaagg acgatcgatg ggaagatcgg gcggagttcg tcggtaggac
    11461 gctgtttaac aaaacagttg gtgtgattgg tgcaggaaat accggaagcg gagtaatcga
    11521 aattttgcgt aacggttttc gctgtgatgt tctagcttat gatccttaca aatcagattt
    11581 atacatcgaa agctttggtg caaaaaaagt agaacttcaa gaattactgg aacaatcaga
    11641 tatcatttgt ttatgtgcga atttgacaga agagaactat cacatgattt ctgataaaga
    11701 agtcgatcag atgaaagaaa atgtttatct atctaatagt gcaagaggcg ctctagttga
    11761 tgaagaagcg atgattaatg gtctgaaaag cggaaagatc gcaggatatg ccacagatgt
    11821 tttagaagaa gaaccgggac gtcaagatca tccgtattta gcgtttgaac aagtagtgat
    11881 tacgccacat atatcggcct acacaaggga atgtttagaa gcaatgggtg aaaaatgtgt
    11941 cagagatgtc gaacagatca gtcagcacct tcttcctgaa cgttctgtcc agacaaagag
    12001 caactatata aaaggatgat gaagaaaatg gtgaacggtt taaaagtgaa aacaggccct
    12061 caattttatt tatatgaaga aggtggtatt tcaaaagttt ctgatctctt gaaatcatac
    12121 ggcgcgaaaa gagtactggt cacccacgga acggtatcat gggaaaaggc gctacctaaa
    12181 ttagtatttt tgaatgacga aacgattcaa tttttttatc atcgttattc tggagaatgt
    12241 agttatgctg aagccagacg aatagctaca atcatcaaaa aaatgaaatc gatttcgtag
    12301 ttggcgttgg cggaggaaaa ctgaccgatc tagtgggtta tagtgccaat cttgcaagaa
    12361 tcgatttcgg agtcatccct actttagcta gtaattgtgc tccatggaca cctcttgctg
    12421 tcatgtataa ggagaatgga caagcagaag ggaaaacaga acacttttac agacaagctg
    12481 ctttttttat aacagatccc caattagtga ttgattcccc tgttccatat ttcattgccg
    12541 ggttagcaga tacactagca aaatggtatg aatctgagct catcttagaa caagattttt
    12601 tgcaagatga atcatttttg aagctagcac aagatactgc gaagatctgc aaagaagaga
    12661 ttttgaatca ttctgctaaa gcaatccaag atatggcaca aagaaaactg acatcggagt
    12721 tcaaacagct ttcagagatt atctttgctg ttgctggttt agttggagga ttaggagata
    12781 aatacgcaag aaatgtggca gcacatgcta tgcatgacgc tatcagtaaa tatattccgg
    12841 aaagccatcg ttttctacat ggagaaaaag tagcgtatgg gattttctat caactggcac
    12901 tagaaggtcg ctgggaaaaa atagacgagt tatatccttt gtatcgggaa ttagaactgc
    12961 cggtttctct agaacaaatg ggattatttc cgaaagataa acagataata gataaaatgg
    13021 ctgaatttat tgattcgaaa gagaaagtgc atttgattcc tatagaggta acacagaaat
    13081 cactgataca gtcaatgatg gaactggaag gttatatcag aaagatctga aggaggagga
    13141 cacatggaaa cattaagtgt agtacaaagt ttgttgattg ctttatgggt agccgcggtt
    13201 atgtcccgct ggttaggtgg aggagccacg ttgaccttac gtttttctcc tttgatgact
    13261 ggattagtcg tcgggttgat catgggggaa gttgcacaag cgatgattgt tacagccgct
    13321 ttgcaaatga tttatatggg ggttttttct ccaggaggat caatgccagc tgaaccatct
    13381 attgcagcag ctatcgcagt accagtcgca ttattagggg atctgtcgcc agaagcagcg
    13441 attgccgttg cggtgccagt cggattgctc ggaagttact tgtatcagtt tcgttttttc
    13501 atcaatactt ttttaggtaa atatacagac aaagctgtag aagatttgaa tacaaaagct
    13561 atggcccgat caattattct ttatccaacg gttgcctcat ttttattatt cgttccgctt
    13621 gttttcattg ctttatattt aggggcgcca ttgatcgctg atgtaatcaa agctttagag
    13681 ggaacagtcg ttatccacat tcttgaagta gtaggtggag ggttagcggc aatcggtatc
    13741 gcgacaactg tatacgttat cggtcgaaaa gattatctgg tctttttctt ccttgcttat
    13801 tttatgagtg ttgttttgaa atctttagat attacgatgg ttacttatgc catttttggg
    13861 attattatcg ctttgatctt tgtccagtcg caaaaaggga aacaagtaca gcctgccaca
    13921 agcaataaca attcagcatc ttttgatgac gaagatgact atgatgacgg attttaattt
    13981 aggaggaatg ggaaatggct gaaataatgg ataatcaaac aggtgctcct gaagaaatca
    14041 cgaaaaaaga tgtaacaaaa gcctatctga gatggcattt tgcaaacgaa attcctcact
    14101 cttttgaacg ttacttggcg ccatcactac tatacggaat gatgccaatc ttgaaaaaac
    14161 tttataaaga tgaagactct ttaaaagcag catataaacg gcagttactg ttcttcaaca
    14221 ctcaattaag ctggggtggc ggtgtgatta ctggcctaat gtcttctatg gaacagcaaa
    14281 gagcagaaga agagtatgaa gaaaaagaga tcttgatgca ggatgacttg atgtataaca
    14341 cgaaagcggg attgatgggg gcactagcag gaatcggaga tgcaatcgat tcaggaaccg
    14401 tccaatatat ttttatcgca atcgcggtac cttgggcaca acagggaagt cctttaggcg
    14461 cactttttcc ttttatctgc tttgggttgt atcaggtttt actaggggta tttttcgcaa
    14521 gacaagcttt cactatgggc cgtaatgcta caggcctaaa gcaaaacact ggtgttcaga
    14581 cagcgattga aatgctatca gttttagggt tgtttatgat gggggtgctt gcgggaaact
    14641 atgtaaaagt agaatcttca ttgaaatttg cgatatccgg acgtgagttt gttatccagg
    14701 aaattcttga tcaaatcgtg ccagggatat tacctttagc tgtcgtaatg ggtgtgtact
    14761 tgttctatac gaaaaaagga ttgaaagtca cacaagcatt attatggctg actggaatat
    14821 taattgtgct agcaggaatc ggtattttat aaaaagaagg gaacgtgaat aagatgggtg
    14881 tagtcaattt agcgcgtgtg gatgagcgtt tgatccatgg gcaagtaatg gtaacgttat
    14941 ctcaaaaaag tggagtgaat tccatttttg ttgtagacga agttgtagca aaagataaat
    15001 ttatgagaga tctttacaaa aatgcaggaa gccgtacagg acaaaaaaca attgtcatta
    15061 caccagagaa agctaaattc tattgggatg aatatcaatt taaagaatac aattgtattt
    15121 tgatcgcaaa aacagtccaa acgatttacg acttagtaaa acacggaatc cccatggaag
    15181 aattgaatat tggagggatt gctcaaaaaa atcctgatac agatctttta gtgacgaaat
    15241 ctgtttattt aaataaatca gatgctgaaa aattgaaaga aatgaatgaa caatatggcg
    15301 taaagaatat ttattttcaa gccactccat ctgcacctaa aacaggatta aaagatgtat
    15361 tgaacaagtt tgatttgtca taaacaaatg cttagaaaaa agataaagag ggcgaaaaaa
    15421 tgaagaagaa cgagctgttt cgtgattggg aatttcgtta tcgctatatt taccgtaagc
    15481 gccgtacaaa aaagagtaaa cagcgttttc tttcagcatt agtatcagat atttacagta
    15541 tgagaacaga tgtgacagtc attgcttacg atacgctagc ctaccgatca aaaaatattt
    15601 atgttggaga tatcgaaaaa gcagagaaag ttatctgtac ttattacgat acacccgttc
    15661 atgcattagg aagttatttc atgtttgatt ggaaagacca aaggaaaaaa acaatctact
    15721 ctatattatt gtccttcatt ttgttgttca gtttaggttg gtggggaatg atgatatata
    15781 acaaaaatcc tcatcacgta ttcgatctgc tatccgtata aacaggtatc actgtactag
    15841 catttgggag ctactttttc ttattgggaa aagcagctcg cggatggcct agcagacaaa
    15901 cttttattcg aaatacttca tccatattaa cgatgctaga aatgatccgt acgattgatg
    15961 atccgagtgt agcgtatgcg tttgttgatg aaggctgtta tggagaaaag ggcttagaca
    16021 gcgtgagatc gggtatgaaa aaagaggcta ttctttttta tctagactct gttggggcag
    16081 atacaccgct acagttttcg ggaaattatt tttcaaataa agaacagtgg ctgaaacaag
    16141 ttgataagtt gaaagaaaaa aacgtcaatt atatttttag tgcaagaaaa aagcaagcgc
    16201 agttcttcta tttgaccaaa acagatttac gagggaaaac atttaattgg caaaatgcca
    16261 atcagattat tgctcttttt cgataagtaa aaagtgcaaa acaaggagtg agaagactaa
    16321 tgttggaaat catgatcgtt acacatggta agttgagcga aggactgaaa gatgcggcgg
    16381 aagtaattgt cgggacatcg agccatattg aaacaatgtg cttagagtct ggggaggaca
    16441 tccaaacatt cggagagaag gtcaaagaaa aattaactat gatcaacaca gcagaaccca
    16501 tgctagtatt tgttgatcta attagcgcaa gtccgtacaa tcaagcgctc ttggcagtca
    16561 atgaactgac tgaagaaaag aaagcagcca cttatgtcat agggggcgtg aatctgccaa
    16621 tgttgttaga agcaatcaat catcagatgc ttcacacacc gatagaagaa gcggtggaac
    16681 aaattgtcaa acaaggaaaa gataatttaa ctgtttggca tgcttcagcg gaagaaaaaa
    16741 aattagatga ggacgatttt tagaaggaag gctgaaaaga tgaagtgggg atttagatgg
    16801 tatggtgaaa aaggggattc gatcccatta aaatatgttc gccaaatacc gggaataaat
    16861 gggataattg gaactttgct gactaaaatg cctggagatg tttgggagat ttcagaaatt
    16921 gaggagttga aagcatctgt tgagagccaa ggactggaac tgcttggaat tgaaagtgta
    16981 gcgatccacg atgcaatcaa ggcagggaca gatgaacgag attactatat tgataactat
    17041 atacgaacaa tcaaaaactt gtctcgatgt ggggttcgat tgatttgtta tagcttcaaa
    17101 ccaatttttg gatgggcgaa aactgatttg tttaatgaaa acaacgatgg aagctattct
    17161 cttttatacg atcagaaaat cgtcgatcag atggagccag gagaaatgta tcaattgata
    17221 cacagccagt caaagggatt caaattgccg ggttgggaag aagaacggtt gaaaaaattc
    17281 gctcagctgg aaaaactgta tgaaggaatg accgaagaag aattatttga aaatctgact
    17341 tattttttag aaaaagtgat tccagtgtgc gaaaaagcag atgtaaaaat ggctatccat
    17401 ccagatgatc cgccatggga actttttggt tttcctagag tgacgaaaaa tcttggcgac
    17461 ttgaagaaaa ttctaaatgc agtagattct ccatataatg gactaacgct gtgcacagga
    17521 tctttagggg caaatccgga aaacgattta gttgaaatca tccatgaggt aggttcgcgc
    17581 atccattttg tccacttcag aaacgtggag tttttaggag aaagaaagtt caaagaaacg
    17641 gcacatccct ctacagaagg ctcccttgat atgtatcata ttatgaaagc gttggtgcaa
    17701 gaagggttcg acggtcctat acgccctgat catggtagaa ccgtgtgggg tgaagtggct
    17761 atgcctggat atggcctgta tgatcgtgca atgggactca cttacatgca aggtctatat
    17821 gaggcgataa gtaaggaaaa accactggga gagcattatg aataaaatca agctcttcat
    17881 ctttgatatg gatggccttt tattagaaac tggacgttta gcttatcgtg cttatgtaaa
    17941 atcagcacaa aagtatgatt atgaaatgcg taaggaagtc tattatttac taactggcca
    18001 aacagaaatg gcgataagag aacaaatggg cattttgtac ggagaagatg ttccatatat
    18061 caaatggcga gaagcaatca atcaatataa agaaaaaatc gtgaaagaag ataagagagt
    18121 atatacgaaa aaaggagcag aagaaatttt atcttttgca aaagaaagag gaatccatac
    18181 aatcgtggct tcgtctaata cacgggtaaa agtagaaatg tatttaaaaa tggaaaatct
    18241 atatgccttg tttgatcaaa tcatttcagg ggatgatgta aaaaaaggaa agccggaacc
    18301 agagatcttt ttgaaagctt gttcaaaaat gaatattcct ccaagtgaag cacttgtatt
    18361 tgaagactcg attgcaggga tcgaagcagc aaggcgagcg ggcattttgt cttttctcat
    18421 caaagatcat ttgatagggt tgcccgatca taatgggaag cataagttga aagtagatgt
    18481 aagtttatat gaatccaata aagcagacta tgaattttat gacctgcatc aagcaaaacg
    18541 atttttagaa agtaaagggt tgtatctcta ataaagagga ctattttcta gtcctcttta
    18601 tttattgtcc tgcacgactg agggaacaga tgaaaaagaa gtaatttttt attttgaagt
    18661 atctacctta tttgtagtga acaatttaga acaaacacaa aacgcatcta ctgttatcta
    18721 ccagtagatg cgtttaaaga gaagaacgag aatcgtccat atgagggttt ccactcctta
    18781 tgcagatttt tttaattctt gcactgcttg ttcgatcgta acacctgtcg ctacatgatt
    18841 ggtattttct ttgtcaaaaa cgacaaatag attcaaatct gtatccaagg ttactcgata
    18901 ttgtatatct ttgttataga atgtcatagc cattctcctt tctttaaata cttgctgaaa
    18961 ggcaacgaac gcgttgataa cgaaattcca tttataagta taccaccaat tgaaaggttt
    19021 tcatagttaa gaaattttaa agtcaaaagt tgcagataaa agcaaataaa caagaaaaca
    19081 aaaagtgaat caatcgcggt tattcaactt tttcttcgat tcgaagacaa tagaagacaa
    19141 tgaattttat atttcattgc attatccttg tgttattaga aaaagcatga tatattaaat
    19201 gagtaatcta ttttgaaacg taatttgagc gatatatcta catagataaa actccttttg
    19261 cctatcaaat atttattgca acgaaaaaac gtacattgat acgtatcagg aggaatttat
    19321 ttatgaataa cggtacagta aaatggttca acgcagacaa aggttttggc tttatcactg
    19381 gtgaagatgg aaatgatgta tttgcacatt tttcagctat ccaaggtgac ggcttcaaaa
    19441 ctttagaaga aggccaagca gtgtcttacg atgttgaaga tggtcaacgt ggccctcaag
    19501 caacaaacat tgttaaagct taataaaaat tgacttttaa ccgtcttttc gaaagaaaa
//
LOCUS       DACDUU010000058        19488 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.058, whole genome shotgun sequence.
ACCESSION   DACDUU010000058 DACDUU010000000
VERSION     DACDUU010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.058"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..305
                     /locus_tag="IWL30_002036"
     CDS             <1..305
                     /locus_tag="IWL30_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9706775.1"
                     /translation="IDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSS
                     FSSFLKIIIGSGIILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            298..2022
                     /locus_tag="IWL30_002037"
     CDS             298..2022
                     /locus_tag="IWL30_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9706776.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            2019..3470
                     /locus_tag="IWL30_002038"
     CDS             2019..3470
                     /locus_tag="IWL30_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9706777.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(3943..6096)
                     /locus_tag="IWL30_002039"
     CDS             complement(3943..6096)
                     /locus_tag="IWL30_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP9706778.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(6098..7144)
                     /locus_tag="IWL30_002040"
     CDS             complement(6098..7144)
                     /locus_tag="IWL30_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9706779.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            7294..8583
                     /locus_tag="IWL30_002041"
     CDS             7294..8583
                     /locus_tag="IWL30_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAP9706780.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            8596..11298
                     /locus_tag="IWL30_002042"
     CDS             8596..11298
                     /locus_tag="IWL30_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP9706781.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            11283..12140
                     /locus_tag="IWL30_002043"
     CDS             11283..12140
                     /locus_tag="IWL30_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9706782.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(12211..13068)
                     /locus_tag="IWL30_002044"
     CDS             complement(12211..13068)
                     /locus_tag="IWL30_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP9706783.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            13279..15450
                     /locus_tag="IWL30_002045"
     CDS             13279..15450
                     /locus_tag="IWL30_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP9706784.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            15518..15859
                     /locus_tag="IWL30_002046"
     CDS             15518..15859
                     /locus_tag="IWL30_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP9706785.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(16135..17889)
                     /locus_tag="IWL30_002047"
     CDS             complement(16135..17889)
                     /locus_tag="IWL30_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9706786.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLSFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(17900..18736)
                     /locus_tag="IWL30_002048"
     CDS             complement(17900..18736)
                     /locus_tag="IWL30_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9706787.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(18738..19421)
                     /locus_tag="IWL30_002049"
     CDS             complement(18738..19421)
                     /locus_tag="IWL30_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAP9706788.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
BASE COUNT         6438 a         3515 c         4022 g         5513 t
ORIGIN      
        1 ctattgattt tattttactt tttgccttgc tctgcctatt tgctacttat caatacagta
       61 tgattttaga tagtgaatat acgatctctg ttcctaatct gctaaagcta agttttattt
      121 cggttttttc cagcttttcg tcttttctaa aaataattat tggtagcggt ataattttag
      181 gcgtgacttg gcaattcaaa ggattgatcc tgtttggtgt cattggtctt ttgacggttt
      241 ggaatggtac gatgacaaca cattggcgtg aagaattaga taaacagttg gaaagttatg
      301 aataagtttc ggattctatt ttcaaaagga accttactaa atcgtctgtt gcggaaatac
      361 gcggttatca tgatcggtgt aacgatgact gctaccgtta tttttagtat ccatacttgg
      421 gaacaaaatc aaaaacaggc agaaaacatg acaagtgatg ccgttcaaag cacaagcaga
      481 atgctgaatg ataaaacaac cttgagtagg atcatcaaaa accagcttgt agggaattcc
      541 gaaaagatcg aaaatgtgac gacctactta acaaaaccca tagatcagta tctgatgtat
      601 gtatacgaac agcaaaacag taccgatgaa ttagtttctt tcccaaatca aatcaaagat
      661 ctctacgcaa attatgaaga actaagtgcg atatatatcg tgttgaatca actaccggag
      721 tattatgaat cttctagaga aaacaaaggt ggggaaataa aaaaggggac accaaaatta
      781 ctagacgcct tttatatcaa gttgcctatc acacaaggag gagctgagtc aggaagtatt
      841 tttattggtt ttcaaaagaa agaactagat atgattttag aaaatctaac gtcattcaat
      901 ggactctctt tatacatgat ttctggaacg acaaatcgac tatacacatt ccatgataat
      961 aaaatagcta aagaaacgtt tgaaagacaa gaaatgatca tcacagagag tctgaagaaa
     1021 aattcacaat tgccgattga cgatctggaa aaaaacaatt ttgttcaaca ccaagaactg
     1081 tctgatgatt atcgtatatt agctgtacta gataaaaaaa gtgtaaaaat cgaaacggga
     1141 aaaaatcttg ccccgctgat cattggtatc attattttag acaatatact gcttatcttt
     1201 ctttataaaa cattcaaacg atattcacag caagtatcta tcgtcatgca ggccatggat
     1261 caaacagcag cagggaattt agagacgcgg atcgacataa caaacacgga atatgaactg
     1321 aaggaacttt caatcggtat caacgaaatg ttggatagca tcaatcagtt tgtagaagac
     1381 atctacaaat tagaaatcaa acagcaagac gcacatatgc gggcgcttca agcacagatc
     1441 aatccacact ttttatataa tacattagaa tatatccgaa tgtatgcaat cagcgaagga
     1501 agtgaagaat tagcagatgt tgtgtatgcg ttttccgctt tgcttcgaaa caatacgaat
     1561 caagagaaaa cgatcacttt aaaagaagaa ctggactttt gcgaaaaata tgtctatttg
     1621 tatcaaatgc gttatcctaa tcgagtagcc tatcatttta tgatcgatcc tgatttggaa
     1681 aagatcgaag tacctaaatt tgtgatccag ccattagttg aaaattattt taaacacggt
     1741 attgatttta ctcgattcga taatgcgcta agtgtcaaag tcttgcagga aggcaaacgg
     1801 gtaaggatca tcatcaaaga taatggaaaa gggatgactg aaaaacggct aaaacagatt
     1861 gaagaaaaac tgtctcatcc taaagtggaa cttcacggtt ctatcggtct gcaaaacgtg
     1921 aatgaacgtt tacgtgccag cttcggcagc agttattata tgtcgcttga gaataatgag
     1981 acaggtggac tgaccgtttc tattacattc aaggagggat gagcgtgtat caagtgttat
     2041 tggtagatga tgaatacatg attttgaatg ggttaaaaaa gataattgat tggcaaagtt
     2101 taggctttca aatagtagca acagcagaga atgccaaaga aggactagct gtacttgaac
     2161 agcgacagat tgatttagta gtaacggatg tgacaatgcc agaaataaat ggactggaat
     2221 ttattgaagc ggctcaaaaa gaacgtcata attttgaatt tatgatttta tcaggatacc
     2281 aagaattcga ttatttgaaa ggaggtatgc aattaggcgc cgtgaattat ttgatgaaac
     2341 ccgtgaataa gttcgaattg gttgaaagcc tcaagaaaat caaaacacgg ttagatcagc
     2401 aaaatgaaca aaaaaatcaa caagaaatct atcaagaact gttattcagt caatggttga
     2461 atgaagaatt ggatgaaata agtgaagaag aaataatagg acaaataagt gagaagcagc
     2521 ataggatagt attgattcaa ctggcaagaa ctcatgatac tcttatcaat agatggttgc
     2581 aagaacggca acaaatcttt tactatcaac gaaattacgg agaccgtgta ttgatcacgt
     2641 tgttgttgga ggcagatgaa gttgatggct tctgttgttt tgttcaagaa aactttcctg
     2701 atcaggaatg gctgatcagt atcggggaag agacagattc tttggacaaa atccctgaaa
     2761 cattccaaca agcgaaagac aatctacagt tgcatcaatt ttatggagat aaagacgagc
     2821 atgtttttta cgcagtacaa gcagaaacaa aagatcaaac catcgatttt tcaagtttca
     2881 gacgcgtgct gcaaaataaa caattggaag tcgctcaaaa aatgatcact gatttttttg
     2941 aacagtttca gttagcagcc atgatgccgg aagatatacg atattccagt tttttactgt
     3001 ttatggagat tcaacgggaa ttgattgatc tggaagatga ggaatattta caaggaatcg
     3061 aaaaaatcca tcaggcaaaa acggtgcaag aacttcagca gctacttctg tcattcatca
     3121 aaaagcatca gagacagaaa aagtacagtg acaatgtgga aaaagtaatc gtaatcctgc
     3181 accagcatta tcaagaacca cttacactca aagaagtgtc agaaaacctg catttaaatg
     3241 tcatgtatct aggacagctg ttcaaaaaag aaacgaaaaa aagtttttca gcttatttga
     3301 accatttacg tatggaaaaa gcgaaacaac tgcttttgca cagtaatcaa aacatcaatg
     3361 agatagcaag tgagattggc tacaacaaca ccacgtattt ctctaaatta ttcaagaaaa
     3421 tcgtcggcag atcccctaaa gagtaccgag aaaacagtgg agaatcataa aaaaaaaagc
     3481 caatgtaatg aaataactta taaaaaattg tctagaaatc taattttggg ctgttcttat
     3541 tacttgaggc aaggcagctt tttcatgacc tctcgataaa cggagttcaa aagccagttt
     3601 ctcggtatta agccaaattt atgcaaaaat gtggaaagcc attttcacaa aatttttctt
     3661 aatactcgaa actaaacagc tttttcacga cctctccact aaatggtgtt caatcagctg
     3721 accctcggta ttagctcaaa aaatggcaaa atatgaggaa ctattttgcc atttgtctct
     3781 ctaatactca ggtcagaacg ctgatttcac aacctctcaa taaacggtgt tcaaaagctt
     3841 gcatctgcgg taataagacc aaccaaacga aaaatatgag gatctatttt tgcttggctg
     3901 ttcttattac ttgatgcagg acagcttttt cacaacctct tgttaattat agtttgataa
     3961 ggaaaaaggc aaatcttttg cttgatattt cttagggtga gggatgatcc ctaattggta
     4021 agagatcgtg ccgcctttca gcaaatcttg atgggtaaaa tataatttcg tatattctct
     4081 gtccatccgt ttgattttat ggataaattg ttgctgcggc cgattaggcg ttgcttcaat
     4141 cgtcaattcc tttccatttg acaaaagcaa aacagccttg tcaattaatg gcataccgat
     4201 aacatattca ccggttccag gcgtgacagg ataaaatccc agactattga agatatacca
     4261 agcggccata cttccattgt cctcatctcc agggtaaccc attggagaat cgtcgaaggt
     4321 ctgagtcatc aactgtttaa gcagtggctg tgccatttca ggttgtccta tataactgaa
     4381 caagtaagga tagtgaaaac ttggctggtt tgaaatagct aattgtccaa attctatcgc
     4441 ggccatctca ctcatttcgt ggatctcaaa accatatcct cctgggtcaa acgttggggg
     4501 ctgattgcac agctcaatga gtttttcttt atatctctca ctgccaccat attttccgat
     4561 cagtcctgaa aaatcgtgat aaacagcaaa gctgctttgc caagcacttc cttctgcata
     4621 atcttgtccc caacgattag ctgaaaatgg ttctctgaac ttgccatctt tatcttttga
     4681 acgcataaat cctgactcat agtcaaagat attttgatag ttcaatgatt gctgctgata
     4741 tttcttcgtc atttcctctt cttctagtac cttggcaact tggctaatac aaaaatcgct
     4801 ataagcatag tctaatgtgt gattgactga ttcgtggtag tgattaggaa catagccata
     4861 tgtcaagtaa tcttctgttc cttggcgtcc ataatttgga tgtttgcttt gaaccgtagc
     4921 acctttttcc attgccgtta gaaattcagg catcaagtct cgtcggatat cttttacagc
     4981 agcatctgca atgaccgcat cgatcaatgt cccaggcata agtccacgtt catcaggaga
     5041 cagccatttt ggcaaatagc cactttctct atagctgttc aaaaaacctt ccagcatttc
     5101 ttcgtatttt tctacagcaa tcaaagaata aagaggataa accgttttat acgtatccca
     5161 aaacccatta tttgtataga gaacaccagg ttttatagat cgactagtcg tatcatagtg
     5221 gattttttgc ccactaggat caatttcata aaatgtttgt ggaaataaaa acacccgata
     5281 taaattatga taaaacgtcc gcaattgttg ttgatcatgg tgtgtgagct gtatacgatc
     5341 aaaataggac tgccattgtt cttgactgtg agcaaggtaa gtctctttgc tttggttttg
     5401 ttctagttcc aggttataag ctgcttgttc cgagctgata aaagatgtcc caaatcggat
     5461 agtttgtttt tttgtctgtt caaaatacaa cgggatcgag tcgctatttt tgttggaaac
     5521 agctaactca tcaattgtta aaggatattc aaatctcaat ataaaataaa aagaaaaatc
     5581 aggatcttct gaaccagcaa aatgtgtgat ttttccttcc actgtgtaag gatctataac
     5641 aaagagctga tggcgtcccg gaaaggataa aagtaaaccg ctgtcatttt tgctgtaatc
     5701 aatagataag attccgccat acatgctagg tatcaatgta gaacagatgc cataacgtaa
     5761 ttgacagatc gacagatgag tcgggcaaaa tatactttct tctgggcggt aagaactttg
     5821 agcatggaaa agggtgttct cctgtagttt tccattgatc ggcgtaaaca gcatatgact
     5881 gaaatcaccc atccaaggac ttggttgatg ggtaagacgg tagccttgaa aaacgcggtc
     5941 atcaggatga aaccaccagc tgccgttttg atccgtagtt tgcggtgcaa aataattcat
     6001 cccaaaagga acgccagtat aaggctggca gttcccatta gagaaatttg gctgattcgc
     6061 tgttgcatgt ctagtatcta tttgaccgat attcatttta tttctccttt gttgattgtc
     6121 ttttgatgaa tgaaactgga aatttactat cggatggtcg atcttttgtc tctaaccaag
     6181 tgaccaaagc atctcccgca agcttaccta actgttcaaa atcttgtgcg atggttgtca
     6241 gtggcgggtc aacaagagct gctgcttgga tatcatcaaa tccgataatt gaaaaatctt
     6301 cagggatatg ataaccatgc tgtctcaaga gattcatggt ctggatagcg acaacatcgt
     6361 tttcacaaac aaaagcagtt acttcatact tagtgatata agaaagcaat tcatctaatg
     6421 tcgcgctttt gtcatccagt gaagtgtgga aagtcaaacc agctttgtcc aatgcttcaa
     6481 ggtaaccaag gtaacgctgt cgaacggatt gtggatgagt ctgcgtacca aacaaataag
     6541 cgatttttga atgtccctgt tcgatcaaat aagaggcagc aagcgcaccg ccttggaaat
     6601 tatcggaaag aacggtagga aaaggtaact catagatttt tttatctaat atgacgacag
     6661 ggaattcttt caatgaaagc tcaaaaagta agtcaagatg ttgttctgta tcaagagcat
     6721 aataaatcaa tccgtcaaat tcattgatta gctcttttgc tgttttattt tgtagaaagt
     6781 caagcgtcgt catcatgaca tcaatctgtt tatcttgcat cacagggttc aatcccttgc
     6841 taaaatcacc aacagacaaa tcagcgacat atggaagaag aaacagaatc cgtcgtgttt
     6901 ttgtaggaga agcaagaatc tgttccggag aagcggattt aacgaaactg cctttcccgc
     6961 gaacgcgata aataagtcct aattgctcta attcagtcaa tgcacgtttg gaagtgattc
     7021 tgcttacttt ataggtacgt gaaagttctt tttcagttgg tagttggctg tctacaggta
     7081 attcaccagt taaaatagcc gtttgcaaat cattaaatat tgtctggtac aatggttgag
     7141 tcatataaac acctcttaat gatatatcat attcaaataa taccataatg gatgaaaaag
     7201 taaataaaaa gatatttttt gaaagcgtag acataattgg ctgtatgaag tataatcttt
     7261 aaagatatat caaatagaaa gaaggtttta ttagtggttt ataaagatat accaaaatcg
     7321 gtgcagaaat ttatggaaga tatcacacaa aaatgtgggg atatccatgc ggactgggca
     7381 aagaatttta cagcagcatt tgccaatact ctgttgacaa cagtcaaaaa gcaaaatgac
     7441 ggaaccacct ttttattgac gggagacatt ccagcgatgt ggttaagaga ctctactgca
     7501 caagttcgtc cttatttagt gattgcaaaa gaagatgagg aattagctga catgatcagc
     7561 gggttagtca aaaagcaatt tttttatatc aatatcgatc catatgcgaa tgccttcaac
     7621 gaagaagcta acggtgctgg ccaccaaaca gatcatacaa aaatgaacga ttggatctgg
     7681 gaaagaaaat atgaaattga ttctttatgc tacccaatac agttggccta tcttctttac
     7741 aaaaatacag gaaaaactga tcaattcaat caatctttta ttgaaggtat caaaaaaatc
     7801 ttggacgtgt tcaaaacaga gcaagatcat gaacactctc cttatacatt tgtacgagac
     7861 acgacaagaa aagaagatac gctgatcaat gacgggcgag gtgcaaaaac agcttttaca
     7921 ggaatgacat ggtctggttt ccgtcctagc gatgatgctt gccgttatgg atatctggta
     7981 ccatccaata tgtttgcggt ggtcgtgtta ggttatgtgg aagaaatttt tcaaacgata
     8041 ctcgatgata aagaaattgt acagcaaaca cagaaattga agaaagagat ccaagagggg
     8101 attcagaatc acggacgaac ggttaatcaa aaagggcaaa cgatttatgc atatgaagta
     8161 gatggaatgg gaaacgccac agtcatggat gacagtaatg tgcccaatct gatcgccgca
     8221 ccttacttgg gatattgttc tgaacaagag gaagattatc tgcaaacaag aagaacattg
     8281 ttgagtaaag aaaatccata tttctatgaa ggaaagtacg caaaaggaat tggatcctcc
     8341 catacgccag aaaaatacgt ttggccgatt gcattagcta tggaaggaat gacaacgtca
     8401 gacaaatctg aaaaagagcg gatattgaat ttacttgtag caactgacgc aggaacacac
     8461 cttatgcacg aaggattcga tgtgaacaat cctgaaaatt atacgagaga atggttttct
     8521 tgggcaaata tgatgttttg cgaattagtc atggattact ttgatattag agtagaaaag
     8581 taggaggtat cgtaaatgaa aaagaaagta tatatcattt ctcatagcca ctgggatcgt
     8641 gaatggtaca tggcttacga acagcatcac atgcgactga ttgaattgat agacgatcta
     8701 ctagagctat ttgaagttga tccgtccttc aacagttttc atttggatgg gcaaacgatt
     8761 attttagatg actatcttca agtaaggcca gaaaaaagac aggcaatcca acaagcaatc
     8821 aatgaaggaa aattgagaat tggaccattt tatatcttgc aagatgattt tcttatcagc
     8881 tcggaatcga acgtgagaaa tatgctgatt ggcatggaag aaagccgtaa atggggaacc
     8941 cctgtcatgt taggctattt tcccgataca tttggaaata tgggacagac gcctcaatta
     9001 atgaaacaag ctggtatttc agcagcagcg tttggccgtg gtgtaaaacc aattggcttt
     9061 gataatcaag ttttggaagc agaaaactat tcttcccaat actcagagat gtggtggaaa
     9121 ggccctgatc aaacagcaat ctttggtctg ctatttgcca attggtatag caacgggaat
     9181 gagattccag tagaaaaaga agcagctctt gccttttgga agcaaaaatt agcggatgca
     9241 gaacaatatg cctctacgaa ccacttattg atgatgaatg gtgttgatca tcaaccggta
     9301 caaaaagata tatcaaaagc aatccatctc gcaaatgaac tgtttccaga ttacgagttt
     9361 attcattcta attttactga ctatctggaa gcagttcaaa aagatgtgcc agaagactta
     9421 ggctcagtag aaggagaatt gacaagtcaa gaaacggatg gctggtatac tttagccaat
     9481 acagcatctg ctcgggttta cttaaaacaa tggaatacca aggtccagcg acagctagaa
     9541 aatattacag aaccacttgc gacgatggct tatgaagtta gtggaaacta tccccatgat
     9601 caattagatt atgcttggaa gacattaatg caaaatcatc ctcacgacag tatttgcggt
     9661 tgttcagtcg attctgtcca tcgggaaatg atacctcgtt ttgaaaaagc agatgaagtt
     9721 ggaaagtatc ttgcacaaga ttccttagaa cagctgaccg cagcaatcga tacgaccgga
     9781 ttcccaaaag acagctttcc atttgtcatt gtaaatactg caggaatgga taaaactggt
     9841 gaagcagaaa taaccatcga attagaaaga aaaagattcg ctgaaggaat acctgaacaa
     9901 ctatatcaag agttagaaaa tttgcctaaa cgtaagtatc atgtagaaac aaaaagtggg
     9961 gccactatac cagctatttt atctgaagaa actgtccaat ttggttatga tttgccgaaa
    10021 gaccgtttcc gtgtgccata tatggcaaga atgatcaaag tgactttacc attagaaaac
    10081 atgccagcat tttcttggga aacattcgcg cttgtagaag gggaagcgaa agcagaagaa
    10141 aaggaaacaa tgattcatca gagtggacga atcatcgaaa acggccctct acatttgacg
    10201 attgaaaaaa atggtaccat tacaatggaa gatcgcaaaa acaaacgaaa gttgaataat
    10261 ctgcatattt ttgaagatat cggcgatata ggaaatgagt atatctttaa acaacctttc
    10321 tgtgatcagc caatcctatc cagtaacaaa gaaaactctg aagtaaaagt attagtagat
    10381 acaccggaaa tagcaaaaat aagtattcta caggaaatgg aaatccctgt ttctgcagac
    10441 gagcgtttag aaaaagaaca gcagatggtt gtggaatttc gttatcgaaa agcagaacga
    10501 tccaaagaaa aacgaatcct tcaaataaaa acaatcatga caatcagaaa agatagtaaa
    10561 aaaatcgact ttgaaacgac tctcgacaat cagatgaaag atcatcgctt acgtgtcttg
    10621 tttcctacaa agttacatgt ggaacatcac gaagcagata gtatttttga agtggtgaag
    10681 cgcccaaacc atgtgtctaa aagttgggaa aaccccacga atccgcaaca tcagcaagca
    10741 tttgtcaata ttcatgatga agagtacggt gtaacagtcg gaaactttgg tttgaatgaa
    10801 tatgaagtaa cagaagacgg acaaatcgca gtgacgctac ttcgcagtgt gggagaacta
    10861 ggcgattggg gatattttcc aacaccagaa gcacagtgtc taggtgagca tcggttcaat
    10921 tacagcattg aactacatgg accagaagaa aaattctcta cttatttgca tgcatacgca
    10981 gcacagattc ctttttccac tcaacaaatc aaacatcatg aaggaacatt gatttcaaaa
    11041 caacaatatt taacgataaa aagtgaaacg tttgcaatta cagctttgaa acgcagcaag
    11101 tttagtgata aagtagtagt aagaggtttc aatatgtcta gtcatttaga aaaattggaa
    11161 ataactaaag acaatggaaa aactgtcata ttgaatctgc ttgaagaacc aacgaagcaa
    11221 gcggttgtcc caataatcca accttatgag attcggacaa tcggattcga ggaggaaaac
    11281 gaatgtatgg cggaatagaa gcaggcggaa caaaatttgt gtgtgcggtt tcagatggtg
    11341 gagaaatttt agaaaaatta agtatcccta ccacaacacc agaagaaaca ttggatcagg
    11401 tatttgaatt ttttgatcgt tatgaattga agggaatcgg gattggctca ttcggcccga
    11461 ttggtatcga caaaacaaaa gataattacg gatacgtttt ggcaactccc aaaaaagggt
    11521 gggcacaatt cgattttctt ggcacaatca aggaacgcta caatattcct gctgcttgga
    11581 caacggatgt caatgctgct gcatatgggg aattgaagca aggagcagca caaggaaaaa
    11641 atagttgtat ttaccttacc gtgggaacag gaatcggtgc aggtgtcgtg atcaatgaag
    11701 aaattttttc agggatcgca catcctgaaa tgggacatat ttgggtgaaa cgccatccag
    11761 atgaccatta tgaaggtact tgtccttatc ataaagattg tttagaagga ttagcagcag
    11821 gaccgtctat tgaggcacgc acagggatca aagggcaaga tctgccgcaa gatcatcctg
    11881 tttgggctat tcaggcttat tatattgctc aagcactgat caactatacg ttgacgttag
    11941 cacctgaaaa gattatttta ggcggtgggg taatgaatca ggatcatctc cttcaaaaaa
    12001 tccgtcagca atttgttgaa ttgatgggtg gttatatgga aacacctcaa gtaagtgaat
    12061 atatcgttcg ttggggactg ccgaatgaaa gtgggattat cggtagtttg ctgttagcag
    12121 aacacgaaca tcaaaagtaa gcagacaaat tacacaaaaa gaacagagtc ttatcattat
    12181 ctgctgataa gactctgttc ttttttattt ctattcccat aatccaggta aagctttacc
    12241 gactattttt tcttgagtaa atggatggat catgtgcagt tctaatgcat ggagcatcag
    12301 tcgttcagct gttctatttt ggtagagagg gtctccaacg atcggatgtc ccttatgagc
    12361 taaatgaact ctgatctggt gtgtacgtcc ggtttccaaa acacaataga cttgtgtttg
    12421 ttcagaaagt actttgttga cagtgacatg tgtcaaggcg ctctgaccat ttttatcatc
    12481 tacgaccctt tttctccgat catggcgatc tcgtccaatt ttattggtga tggtcatatc
    12541 tttggcaatt tctccccata cgatggcttg atatctccga tagatcattt tttgttccaa
    12601 cattcttcca agtatcggca agacaaatgg attttttgca aataaaacgg caccgcttgt
    12661 ttctttgtcc aagcggtgga caacgtaagg tcgttgctgt ttagtgtcga gataagcagc
    12721 taaatgattg agtaaggtac tgttttctgt cggttcattt ggatgagtct tcattcctgc
    12781 aggcttatcc actataatca aatgctcatc ttcatataaa acacggatgt gcttcgcatc
    12841 tcctaattga acttcttgat aattataatc tgtttcttcg aataacaatg tgatctggtc
    12901 attgcccttt acttcatgat ggaacaaagc aggttgttga ttgatccaaa cgtttttacg
    12961 tatacgcaaa aagtgtcgaa cctttctagg aactaaccat tccttttcaa gcaagtcgcg
    13021 aattgttgtt tcaggatggt tcataggtaa agtgatcgtg attttcacaa gtgtactcct
    13081 ttcagtgcaa ttgcagacta attgtaccat ttttaaaaca tatttaagaa aaaacttaca
    13141 tttttctgtt cattcttatg ttactctact tgcactaacg taaaaaaaca tattgaacta
    13201 caacttaagc aggaagaaaa cactctttca ctatggtaaa ataagacgat tgaatcaaat
    13261 agacaggaga agaagcaaat ggattttcaa agcattttag aaaagataaa agctgctttg
    13321 atcagttttt ggcgttggat cagaccatat ctcggacaat tccatcgatg gcgaaagcgc
    13381 atctggaaaa aatatcatgt gaacaaattg attttgctga ttggtttgat ctgtgtctta
    13441 gtgacaagta tctacctctt tattttagca aaacaagcaa atgtcgaaac gttaaaatca
    13501 ggattaagcc aatcgacagt catttatgat aagaaaaacg aagaagctgg cacactgtac
    13561 gcccaaaaag gaacatatgt agaattaaac gcgatttctc cctatatcca agatgcagta
    13621 atctcgacag aagataggaa tttttacgag catcacgggt ttgatatcaa agggattgcc
    13681 cgtgcagcag tccgaatggt cttgtctggc ggaacatcag ggggcggagg aagtacgatc
    13741 actcagcagc ttgccaaaaa tgcgtatctg acattagacc aaacgtttga tcgaaaagcg
    13801 aaggaattat ttttggcaat cgagatcgaa aagaagtaca gcaaagaaga aatcttgacc
    13861 atgtatctta acaatgccta ttttggcaac ggggtatggg gtgtccaaga cgcttccaga
    13921 aagtattttg gagtagatgc aacgaatgtt acgctgtctg aggctgctac tttggcagga
    13981 atgctgaaag gtcctggtat ttataatccg atcgatcacc caaaaaatgc gaatgatcgg
    14041 aaaaacacag tactaagtgt aatggaagaa aacggaaaaa tcacaaaaga acaagaacaa
    14101 gcagaaagcc agacagatat cagcgcgtat cttaatgaca catacgataa ttcagattct
    14161 ggttatcgtt atccttatta ttttgatgca gtgatcgatg aagcagttaa cgagtatggt
    14221 cttgatgaag aagatgtcat gaataaaggc tataaaatct atacagcttt agatcaggat
    14281 tatcaaaagc aattagaagc tacttatcaa aacagtgcat tattcccagc aaatgcttca
    14341 gatggtgcca tggtccaatc tgcttcagta gctttagatc cgaatacagg tggtgtacaa
    14401 gcactagtag gacgaagagg agatcatgtc tttagaggat tcagctttgc tacacagatg
    14461 cgtcgttcac caggatcaac gatcaaacca ttaagcgtat atacaccagc actggaagct
    14521 ggatacaagc cttccagtat cttgaaagac gaaccgcaaa gttattacga tgcgcataat
    14581 tttgacggga cataccaagg agaagtaccg atgtatcaag ctgttgcaca aagtttgaat
    14641 ttgcctgctg tatggctttt gcatgagatc ggtttacaaa aaggatatga taaagcagaa
    14701 gaattcggcc tgcctttagc aaaatctgat aagtattatg gcttagccct tggtggactg
    14761 gaaaaaggag tatctccatt gatcatggcg ggtgcttaca gtgcatttgc caatgatggt
    14821 cagatgtata cgccgcatct tatcacgaaa atcgttgatt cgactggagc ggtgatcgtc
    14881 gataacacaa acaaaaaacc aaaacaggtc atatcaaagg aaacagccga tgaaatgacc
    14941 agcatgcttc ttggtacatt ttcaaatgga acagcggctg ctgcgaatcc tgcaggttat
    15001 acgatagcag ggaaaacagg aaccacagaa actaatttcg atgccacaaa agcaaatgac
    15061 caatggatga tcggttacac gccagacgta gtgatttcga catggatggg ttacgaagaa
    15121 tccagcaagc tgcactattt ggaagggaca agtggaaatg ttgtcgggaa agtcttcaaa
    15181 tcagcggcag aaggcatcct tccgtatact tcaaaaacag gatttagtgt agcagatgcc
    15241 tatgccacag gaggacaagt ggtagctgca gatcaagttg gaaacacagg aaattctaat
    15301 ggagagtctg gaaattggca agataacttg aatcaatatg gtgaacaagc aaaagaaggg
    15361 ctgaaaaact tcggcgatat ggtgaaagaa ggcgttcagg gaattggtga agcggctaaa
    15421 gacctctggc ggaaatatca aggagagtag cccctttgaa aacgaataac cccatgttac
    15481 aatagagagt aactactaaa agaaaagagg gcataacatg tcaaacatct acgatagcgc
    15541 gaatcaaatc gaacgtgaga tccgtgaatt accagaattc aaagcgttga aagaagcgta
    15601 cgagaaagta caagcaaacg aagaagcaca caaactgttc aaagacttcc aagcgatgca
    15661 aatcgaatta caagaaaaac aaatgagtgg acaagagttc agcgacgaag atgcaatgaa
    15721 agcacaagaa atggctgtga aaatccaatc agaagaagta atcaatgact tgatgcaaaa
    15781 agagcaaggc ttcagtacaa tcatcaatga cttgaaccgt atcatcatga caccagttcg
    15841 cgacctttac agcgaataaa actattttat gaaacaacag agggtgtgaa agagacgttc
    15901 ggccttgagc gatgacaaaa ttgcttctta ttgccgatag gctgtctctt gaacaccatt
    15961 taccaagagg tcgtgagcct accgtttaac tctgagttac aaggaacaat ttcaaaaaac
    16021 agcttctcat gtttgatgac tattgtgact tgtgaccgag gagttggcag gagaacaccg
    16081 tttattcaga aataggagct acgacaaaag tcttgtcata gctcctattt tttgtcattc
    16141 atttttcaag cggtagaaat agcctttttg agctaacagt ttttcgtaag tacctatttc
    16201 aacgattttt ccttcatcga tcacaacgat tttatctgcc tctttgatcg ttgaaagccg
    16261 atgagccaca ataaaagtag tgggtgtttc tgtcaggtaa ttcagggctt gctggatttt
    16321 tttctctgac tggttatcta aagctgaggt tgcttcatct agcaaaatga tttttggctg
    16381 acgtatcaac gctcgtgcaa tggagataca ttgcttttgg ccaccagaca gcttgttgcc
    16441 atgttcgcct actaaggtat ccagaccatc aggaagctgg tcaatcagtg aagaaagttg
    16501 cgcagcttcg atgacttcat ctaaacgttc ttttgatacg tttttcaagc cataagtgat
    16561 gttctctttg atcgaagcgg agaacaacaa agtggtttga ggaacaacgg ctaaataatt
    16621 tcgcacactt cgcatatcca gttcatccaa aggctgatca tctagaaaaa tttttcctga
    16681 agtcggctgg ataaatccaa tcaataagtt aaggagtgta gattttccag agccagatgg
    16741 cccaacaaaa gcaattgttt ctccagcttt cacctctaga gaaaaatgat cgataatcgg
    16801 tttgagtgag tccggataaa gaaaagaaac atcctcaaaa cgaaaatttc catccaaatg
    16861 ttggattttt ttcttaccgt gataagtttc tacatcgcca gcaatcaaca gttcgccaat
    16921 agaagtgata gattccatgc cttttgaaat cgtaggaatc agcgtgatca ttccagaaac
    16981 ggaattgacg atcgtcgtga aatacgtctg atacatcacg acatcgccgg gttggatgcg
    17041 cccttgtaaa gctaatatcc cagtgaaact caaacaaatc agctgaaata tctgaaaact
    17101 agcccagctg actgatccga aaacagattg caggatatct aagtggaatc ctttttcata
    17161 gatttgagta aaatgcttat ttaagcgagt tgtttcgtgt ttttctaacc catgagctcg
    17221 agcaatcggt gctaattcga tcacttcgat cacttttgca gaagtctctt ccatttcttg
    17281 cctgaagtct gagttggact gtctgatcct tttacggaaa aagagagtaa cgaatgcggc
    17341 gagaggaaca gtcaataaga aaaacatgaa aacaacagga cttttataaa gcgtgatacc
    17401 taaagctacg aataaattga cagcgatatt taatccgctg acaaatactt gagaggacaa
    17461 cgtttggata gcttctacat cacggatgat ctttgactgg attcgtccag attgcaattc
    17521 tttttgataa ggaatagata gttcttggat tttcttgata agggtcgctc ttagtccagc
    17581 ttctacttga cggatcgaag tgctatggta ttttacatga aaataattcg tcaatatatt
    17641 ttgaccaatc agacaagcca tcaaaaaagc gttgaaataa atggcctgca actgagaaga
    17701 atctttcgat gaagctgcat tgattacatt ggcaatagcg atcggcatta accaagatgg
    17761 aagatgtttg ataataaaaa agagtcctga catggcaaaa cgccaataat ttccctgata
    17821 aagatgcagc aatgtctgaa aagcatttgc atgatatttt tgataacttt tctgtattcg
    17881 ttcctccata atcttctcct taacaatctt ggtaaataaa ctgcaagtgt tctattgctt
    17941 caggaacata aatctcagga gtagcttcaa gagtggcgta cattagagga cgctcatatt
    18001 tgatataatg cagtaccggg cggaaatcta aacagccctt gccaacaggt accatgcgga
    18061 tctttccatt ttccatgata tagtctttga gatgaagcgc tgtaatataa ccatgtaaat
    18121 tgtccaaggc acctcggatc actttttcct gatcagcatg attttcttta tgcatcaaat
    18181 tcgcacaatc taaaatgatt tttaaatttg gtgaagctac ttcatcgatt aaacgtttag
    18241 ctaactgata gttatagatt ggatgattga tccctgcttc aacagcaaca gttacgccaa
    18301 aattttctgc atgagcgact aattcaatga tagaatttct ggcgatctga taagcttctt
    18361 cagtaaaatt ttcttttgtg taaccgacag agacactacc ggtttccgta gcaatcatct
    18421 tggcttgata ggcagagcac agagcaattt gctgtttgaa agttgccaat gcttgtgcac
    18481 gaacttcttg atttttgctg gataggttga tatagcatcc taaaatggaa agttcgatat
    18541 tgtttttttg gaagtattca ccaaaatagg cagcactccc aagtgtcatg tcagttaatt
    18601 taagttgttc aggaaatgct ttttgtggtg caaattggat atgggacaag tgaagctgtg
    18661 atactttttg aacgatttct tcacgatttc gaacatctaa atcgtgggca cggatgccta
    18721 aatttaatgg aaacatctca aactcctttt tgtaaataat tagcaagagg cagacgggat
    18781 tcttttatca attcaacaat tattcgggca actttatcag cgccttctgg actaaagtgg
    18841 gtattgtctt ttagtccttc tgggtaattt ttacaagtat caggcataag atgcaggtag
    18901 aatttttttg tctcttcttt tgagaaggtc tggaacaatt cttgtgatct tgagaacaca
    18961 tctaaaagag gaatatggtg tttctcagca aatgcacgca tcgcttttgg atagtctcca
    19021 agtatatctg gattcaatgt tccgttttct aagtaatctc gtcgcgtcac agatgtcagt
    19081 aagaccattt ggacgttcgc tttttttgcg cattcaaaaa atatctgcag attttttaaa
    19141 tagctaccga agggaagcgt acctctttct tcttggattt tctggtcgtt atgtccaaat
    19201 tgtacaaata agaaatcatt cggctgaaac tgtgaagcta gacgggctaa ccttccttcc
    19261 tggataaatg atttggtgct gcgtccattc tttgcttggt tattgatctg gatctcgggt
    19321 gaaaaataag cagagatttt ttctccccaa cctgtctcgg gacgcttgtt ttcatctttt
    19381 tctgccgcag tagaatcacc ggcaatatgg atagtaggca tgttatctct tctttcataa
    19441 aggtttatag gcaatgaagc agaacagcaa gagaagaaga cggagcaa
//
LOCUS       DACDUU010000059        19317 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.059, whole genome shotgun sequence.
ACCESSION   DACDUU010000059 DACDUU010000000
VERSION     DACDUU010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.059"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..456)
                     /locus_tag="IWL30_002050"
     CDS             complement(<1..456)
                     /locus_tag="IWL30_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP9706789.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEK"
     gene            complement(561..1424)
                     /locus_tag="IWL30_002051"
     CDS             complement(561..1424)
                     /locus_tag="IWL30_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9706790.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1620..3719)
                     /locus_tag="IWL30_002052"
     CDS             complement(1620..3719)
                     /locus_tag="IWL30_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP9706791.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(3939..5048)
                     /gene="rpoD"
                     /locus_tag="IWL30_002053"
     CDS             complement(3939..5048)
                     /gene="rpoD"
                     /locus_tag="IWL30_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAP9706792.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(5072..6913)
                     /locus_tag="IWL30_002054"
     CDS             complement(5072..6913)
                     /locus_tag="IWL30_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP9706793.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(7095..9488)
                     /locus_tag="IWL30_002055"
     CDS             complement(7095..9488)
                     /locus_tag="IWL30_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9706794.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(9595..10455)
                     /locus_tag="IWL30_002056"
     CDS             complement(9595..10455)
                     /locus_tag="IWL30_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAP9706795.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLCGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(10514..11035)
                     /locus_tag="IWL30_002057"
     CDS             complement(10514..11035)
                     /locus_tag="IWL30_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP9706796.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            11355..12074
                     /locus_tag="IWL30_002058"
     CDS             11355..12074
                     /locus_tag="IWL30_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9706797.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            12100..14691
                     /gene="mprF"
                     /locus_tag="IWL30_002059"
     CDS             12100..14691
                     /gene="mprF"
                     /locus_tag="IWL30_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP9706798.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(14879..16066)
                     /gene="tuf"
                     /locus_tag="IWL30_002060"
     CDS             complement(14879..16066)
                     /gene="tuf"
                     /locus_tag="IWL30_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP9706799.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            16434..16790
                     /locus_tag="IWL30_002061"
     CDS             16434..16790
                     /locus_tag="IWL30_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9706800.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(17031..18035)
                     /locus_tag="IWL30_002062"
     CDS             complement(17031..18035)
                     /locus_tag="IWL30_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAP9706801.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            18201..19052
                     /locus_tag="IWL30_002063"
     CDS             18201..19052
                     /locus_tag="IWL30_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP9706802.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            19069..19314
                     /locus_tag="IWL30_002064"
     CDS             19069..19314
                     /locus_tag="IWL30_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706803.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
BASE COUNT         5386 a         3992 c         3377 g         6562 t
ORIGIN      
        1 tttttctttt tcatagcatt cctgacagat tccatggaac gttagccgat gatcttttac
       61 taaaaaatga taacgttctt ctactacttg ctccacttca agtaaaagat cctcttctac
      121 ttcctcgatc gtgccgcagt tcaagcatag taaatgatga tggaaatgtt tggcgccttc
      181 ttttctcaaa tcataacggg ctaatccatc attaaaactt actttatcga caattttcaa
      241 ttctgtcaag atttcaaggg ttcgataaac agtagcaagt ccaatttccg gactcttttt
      301 tttcactaaa aagaagattt cttctgcaga taaatgctct ttctcatttt ccaaaagcac
      361 aagcagagta gcttctcttt gcggtgtcag cttaaacccc gcttcatgta gctgctgttt
      421 tgtttttttc atcgccgaaa tagcattcat gaccatttac tcaaccccaa caatttataa
      481 taattacaaa taaaaaaaga tataacacta tgttaattaa aattattata agctagttaa
      541 gaaaaacatg caagggattc tcaattagat tttttgacta gttttgtcac accgttttct
      601 tgtgtaatca atccttgttt caaaagatta cctaatgcgc gcttaaatgc tcctttgctg
      661 ataccaaaag ctttcatgat ttcttctgga tccgatttat ctgtaaagtt gatttgctga
      721 tctgcagaac gttctaaaat ggttaaaatc atttgtgcat catctgatat agcttcatat
      781 gctcttggct tcaatgaaag attcagtaca ccgtctggtc ggacaccaat cactcgacct
      841 tttatctgct cgcctagacg aggttcatta tatcgttcag aaggatggat aaacccaaga
      901 tagaaatctt ccgttaaaac gtacgtacct gttaatttca agcggtatgc tgttcctatc
      961 acatctttgt ttttcatttc ttctgttcca tatttgctta aagatttgaa tattttttca
     1021 tctgctaaac tgccccaaat acggtctttt gcatcaactc taagggaaat catcaagcga
     1081 tccccttttt taggccatag ctctgtcatt gtcggcaatt catcaagaga tacgacaaag
     1141 tctttatctg gaagaccgat atcgacaaac gcacctaaat cttttcgtgt agcagtaacg
     1201 attccaaaag cgtaatgacc gatacggctt ttaggaattt gtgtagtgat ccgattttca
     1261 tgtttttgat tttggtaacc gaatccttca actgcttcac cgattttgtg ttcaccttct
     1321 gatttgctta agccaaaggt ttgtccgttt ttttggacaa agtagttttt ttcattttca
     1381 tcaataataa tacctgtaaa tatagttgct aataaatcgt tcattttttt ctccctctat
     1441 agtgaaaggt tttcttaata catttctttg ctatcataac ataatgttat ctacttgaat
     1501 agctgagcct tggtttggaa aggtgaaaaa aggagtatga caacagttgt gtcatactcc
     1561 cctttttcga ataaacggtg ttaaacggca ggctcacagc ctctttcatt tgcctaaaat
     1621 taattgctcc catttcctcc gaaccatcca gttttacagt tgatggttac gatgtaaaat
     1681 ggcaaagcac tttctccaat gcctttcatc aatgcagatt ttgttgaagt ggcatataga
     1741 ttataatagc cttccccatt ttcgattctg gctttttcta atctgtaacc tccttcaacg
     1801 atatatccac gttggataca tgtagcaatc actttgcttt ggacacctgg tgcccacgtc
     1861 catgctggat tagatgcatc ggcaatatca cttgctctgg cagatttgat tggctgattc
     1921 gctgagtttg aagaggaagg ggtcgaggat gccgcttctt gctgtgctgc ttgtgcgacc
     1981 gcagcttgtt gagcggcttg agctgcagct gctgcttgag cggcttgttt tgcttgttct
     2041 tcagctgctt ttttcgcttc ttctgctttt ttcttttcct cttcttcttt tttctcttta
     2101 tcagtgagca tcttatctac ttgtttcaat tgttcattca actgagcaga tagatctgta
     2161 tttttcactt tttctactgc ttcttttgcc gtcttgtatt ggtcgcgtgt tgctgaatcc
     2221 actacttttc cgtctttaaa gactatagct gttttttctt tagctgtttg caattcagca
     2281 tattgatttt tagcttctgt ttgtgcttgt tgaatcaatt ttccaaaatc tgtgttatcc
     2341 gtttcgattg cttggatctc tttatctgct ttcaacttga tgtcatctgc tagatgatca
     2401 tcgacgatgg caggtgctat gaaccactca ttcacttttt ggatatctcg gatcttagat
     2461 tggatatctt ggtaagttgt ttctaactct gaatatcctt tttcattctt aagactggat
     2521 aaatcatttt ttagttttga taaatcttga tgaatcatat ctgcacgaat aaattgatgg
     2581 tgatcatctg tataaaaggc agcaagtttc tcatgaagtt cttccatttg ttgctgtctt
     2641 tttgcttctg ctgctacttc ggcttctact ttttgatgat ggtcataata agcccagctt
     2701 cctccaccga gtagaagtac tgctgctaaa acgagataag gtactttttt tgattttttc
     2761 ttttgcgctt tatctggatc tgttttttct aagtttgatt cttgatgtgt tttttcagta
     2821 tctggcacga ttgttattga tttcgcttct gcttcaaaca gcttaggctg gttctcttct
     2881 ttttctattt ttgcttcaag atcagtaccc tctttgctta cagattgatt ttcagcatcg
     2941 gattcactat ttatttcatc gggctctgtt gactttttgt ctgtgttttc gagtatttct
     3001 tttttctctg cttccggcag tttattcgtt ttttcctttt ctaattgttt ttcagtaaat
     3061 acttcagttt tttctgtgat atccgttttt acgtctttta tttttactgt ttctttacct
     3121 gtggcgtttt ctctatcaga gtctttttca gtttcttgtt ccttttctgg ctctactttg
     3181 ttttcctgtg caattttttc actgatcatt ttgagcagct ctttcgcgtg ctcttctttt
     3241 gtatcttcac gatgtgcttt gatataagcg gatagtatcg aattttcttc ctcattatcg
     3301 acgatttctt tcagttcagc tactcttctt ttttgttttg attcaagcgt ttcttcttga
     3361 ttctttcctg attcttccga agtctcatga acagttgctt tcttctgttc atttttctct
     3421 atattagtta cttgatcgtg agcaggtggt tcttcgtctt ctggttctgc tttttcgaat
     3481 aattccatta cagattctag aggtaaatct ttcaattccg accattcgat tggatcgttt
     3541 aattctgtat caggaaagac tgcatcttca gttgatttat cctgcatagc tgcatctgta
     3601 tttgtttctt caattgattc ttttatttgc tgatcttctg cagttatttg atgaccgcag
     3661 tctggacaga tagtattctg ctccattttt ttcgatccgc aatttggaca ttttttcatc
     3721 acttagcgat cggctccttt actaggttta cgctaccatt taatctatca tattctatac
     3781 gtcacgccaa tagcttattt ttaggattta cttaaaaata atgaatgaat ttccttccta
     3841 tagaaaaaaa agcttgagac tgcgtagtct caagcttttt ttcttttaaa ataaaccaaa
     3901 cagttttact gatttctctt ctggtcaata gtccacgctt attctaagaa atcttttaat
     3961 tgttttgaac gagaaggatg gcgcaatttt cttaatgctt tagcctcgat ctgacgaata
     4021 cgttcccgag taacaccaaa tactttccct acttcttcca atgttctcgt ccggccatca
     4081 tctaaaccaa aacgtaagcg aaggacattc tcttcccgat cagtcaatgt atctagtaca
     4141 tcttccaatt gttcttttag taactcataa gctgcgtgtt ctgctggact tgttgcttcc
     4201 tggtcttcta tgaaatcacc taagtgagag tcatcttctt caccgattgg cgtttcaagg
     4261 gaaacaggtt cttgcgcgat tttcaagatt tctcggactt tttctgttgg aagatccatt
     4321 tccgcaccga tttcttctgg cgtaggttct cttccaaggt cttgcagcaa ttgacgttgg
     4381 atacgtatca atttattaat tgtttcaacc atatgaactg gaatacggat cgtacgtgct
     4441 tggtcagcga ttgctcgagt gatggcttga cgaatccacc atgtagcata ggttgaaaac
     4501 ttaaatcctt tgcggtagtc aaatttttca actgccttca tcagtcccat gtttccttct
     4561 tggatcaagt caaggaactg catgcctcgt cctacataac gtttagcgat acttactact
     4621 aaacgaaggt ttgcttctgc cagacgctgt ttcgcttctt ggtcgccttc ttcgattttc
     4681 aatgctaacg ctacttcttc ttccgcagtt aaaagggaga cacggccaat ttctttcaga
     4741 tacatacgaa ccgggtcatt gatttttaca ccggttggcg cagaaagatc ttctgttttt
     4801 gcttgttcag cttttttctc agcactcttc aagctgtgaa ttgatggatc accattttca
     4861 tccaccacac tgatacctgc gtcttcaatc ttttggatca atttgtccat ttcatctgca
     4921 ttcaaagtga atggtgtagc aagctgattg gataaatcat catatacaac ttgtcgtttg
     4981 ggtttgtttt ctttgatgaa agctgctact gctgcttcat acgttttttt atctgttcca
     5041 tttgccataa aagaaggccc cccttgagta gctatttggc ttgttttaac tgtttcgtta
     5101 aattgatgat ttcaatcgtc aattcaagct caagctgttt gtttccagtt cgtctggctt
     5161 cttgctgcat ttgttttttt aatgttattt catcagctaa gccagatttg ctgatcacat
     5221 ctagcaagtc ttggatttct cgtggcgaac tttcttctgc caccggtaaa tattcgattt
     5281 ccaccaccaa ccgccttaaa ttttcatctt gcaaaaagtc taaaaactct gcaaggttga
     5341 attcataatg ttgtgagaca tatgcatcga ataaaaaata aacttctgca tatgcatcat
     5401 gaataaactg aaattctcgt tcttttaaaa tattacggac agaaacttct ttaaatgcac
     5461 gatataatag aatctgctct gccttttcta cttgcgtcaa aggtctcttt ttccgtgcag
     5521 tttcttgtct cgtaacctga gtgtgttgac tttcctgagg ttcttgacga ttttgctgat
     5581 ttgtccttcg aagatcgcgc agttgttttt gtatcgtctc tctgccgatc tgaaactctg
     5641 ttgacagttg agatagatac atatcttgtt cgattggtga aacaactcgg ctaagttcaa
     5701 ctagtaattc ttcgaggtat tcgatcttgt ctttttcatt atctaaattt ttcccttgtt
     5761 ttaaataacg tgatttaaaa gtaaagactg tttcgcgccc atgagccact aattcttgga
     5821 aagatgcttc tccatactta cggagatatt catcaggatc aagcttttct ggaacgacta
     5881 cgacagaaag tgctaatcga ctgttttgcc gaagcaattc aatccctcga ttggttgctt
     5941 caaaacctgc gttgtctccg tcatagcaaa agaccaattc tttcgtcagc ttttccattt
     6001 gttggatctg ttgagctgtt agacttgtcc ccatagaagc taatccagtt ttgataccag
     6061 cctgccatgc agcaatcaca tccataaacc cttcgaacaa caaaatttct ccgtttttcc
     6121 ggatatcgct tcttgcttta tcaagattaa ataaaacttg tcttttattg aacagttctg
     6181 tttctggact attcaagtat ttgggctgat ctttttcctg ccctttttca ggtgcaagcc
     6241 agcgtcctga aaaaccgatc gtctggcctt gagaattacg aagtggaaac atcactcttt
     6301 gataaaaacg atcagacata cggccatcat catgcgtgac aaacaatcca gagcgttcta
     6361 acaattcatt tgaaattgat tcattcttga atacttgctg aagaaaagaa cgctcattag
     6421 gtgcaaaacc tatgttgaat tcttcaatta attctaaaga caacccacga ctttgcaaat
     6481 attctaaagc tggctgtcca gcttttgtat gcaatagcat gtgatgatag atttccgctg
     6541 ctttttcatg agcagcaatc agtttccccg tagcagattc aggattttgg acagctatag
     6601 tacgctgacg ccattgatct tctaacggta tctgttcgaa gtctgccact tttaccactg
     6661 cttctggaaa acttagacct tctagttctt gaagaaaagt aaacacgttt ccgcctttac
     6721 cacaaccaaa gcaatgaaaa atctgcttgt cttctgcaac agaaaaagac ggtgtacgtt
     6781 cttcatgaaa aggacacagc cccaagtaat ttttaccaga ctttttcaac tggacatatt
     6841 gtccaatcac ctcaacgata ttcgtttgac tgcgtactgt ttcgataacc tcttgaggaa
     6901 tccgttgagc cattttttca ccccctgctc tattgatatc ttttgggttt aacagaagat
     6961 atttcaggag aataaccggt aaaaaatcgc actaactgaa atcagtgcga ttttaaagga
     7021 cacaattata catgtttgat tgtatcatat ttcattgctt tttcaagtat aacacatttc
     7081 tatctcatta agcatcaatt tgctactctt ttcagtaact gattttgaat atcagccttc
     7141 cagtacaata ctcgaagaac aatatttacg agcatgaata caaagaagta tcctacgctc
     7201 tctcccaacc ctaagtttac ttccaaaatt ccatggaaca gcattgcagc tacatatatc
     7261 cctacgatcg tcgttactgc taggaagatg cgcaatgggt tgaagggata acaagctttt
     7321 acaactgcca tgcaactgat cccaatcagc aagtaataca tcaaagtagt agtttcagca
     7381 tttccccatg cgaatgtttt tccaagtaga tacaccgcaa tgatattcac tactacaagc
     7441 aaggcatttg gtaaagcatt aataagtgct gtaggcaaat aacggtaagt gatttttctt
     7501 ttatcttgtt caaaagataa aaagaaggaa ggataccctt caatcgctaa atcaatcagc
     7561 gtgatttgga tagggataaa cggaaatcca attgctgtaa cggcacatat aatcgataaa
     7621 ataaatgaat aaatcgtttt gataaaaaag atgcctgaga ctttcgtcac attgttgacc
     7681 actcggcgtc cttcaaacag gacttctggc aatgtagtaa aatcagagtc caacaatacc
     7741 agattcgcaa tctgacgtgt tgctccatca ccttctgcca tagcaatgct gcaatctgct
     7801 tctcttaacg ccaaaacatc attcacaccg tcgcctgtca ttgcaaccgt tctgccgctt
     7861 tctttcaatt catttaccag caatttcttt tgctgcggac tcactcgtcc aaaaaccgta
     7921 tagcgatgag cagcttctcg cacttccatt tcttcagtaa tttgagaaag atcgacatac
     7981 gcatcataat tcggtaatcc tgcacgtcta gcgatattgg agacagtaaa aggattgtct
     8041 ccagaaatca ctttcagatc cacaccttcc tcatgaagat aagctagtgt ttcttgagcg
     8101 ttttttcgga ttggatcatc gatttcaaac agagcaaccg gctcaacaaa tgctggtttt
     8161 tcttcgttca attgctgatt tggagcgatc cccaacataa gtacgcgata gccattcgct
     8221 tgggctgttt ttagactgtc cggaagccga tcttcttcca ccaaacgttc aggcgctcct
     8281 acgtacacga ctcccagttc tggaaattcc atcgcgcccc atttgcgatc agaagaaaat
     8341 gcgactttgt ttgtcacatc ataccgatta gaaagctcaa aataatctct catagcccgc
     8401 atagtgatgt tgttatcact gctttctgcc agatacgtcc ccataatttc tgggatggct
     8461 ttctcgtaca gcggatgtag tgcttctacc cgctggatac tcattttccc ttcagtaatg
     8521 gtccctgttt tatccaagca taatgtatcg acatgggcca gcgtttcaat tgaatacatg
     8581 tcttgaacaa ggattcgttt ttttgccagt ttggttaccc ctgtcgttaa tgcaatactt
     8641 attagcaata ccaaaccctt tggaagcatg cctaacaaag ctgctgcaga tgcaataaca
     8701 gaaattttga tagaagcatc gcggatgaac agtgcttcca aaaatagtat gatccccaaa
     8761 ggaataatca cgaaactagt gaatttggat actttgcgaa tcgaatctac aagttcagaa
     8821 cgaatagctt tgtgagtctt tgcctcgcta gtcaagcgga cagcataatt atctgcacct
     8881 actcggataa cttcagccat gacagaaccg cttgtcaaaa agcttccaga aagcaattca
     8941 gctccagttt ctttgacaat caaatcagat tcgcctgtca aaagagcttc atttgcttct
     9001 gcccgtccag atataacctg taaatcggct ggtatctgtt ctcctgctcc gatttttacc
     9061 aaatctccta ataccagctc tttagtagaa atcgattgtt ctttaccgtc ccggatcact
     9121 agtacttctg attgggaaac aatcgataat ttccgtacaa gatttcttgc atgtatttct
     9181 tgataaattc caataacgat attgacgaca ataattgcta aatacgccat gttggaatac
     9241 gcacctacaa ataacagaca aatcccaata ccaaagttca gaaaattgaa taacgtcaat
     9301 atattatcgc tgaatatttg ttttgtactt tttgatgcat cttcctggta gtcattttgc
     9361 tggccagaag ccattttctg aactacctct tgctgtgtaa gtccattcat tttctttttc
     9421 tccattttcc gccaactttc aaaaataatc acgtgtttaa gtctagcgtg ttccaagatt
     9481 aaaaacaact tgtttttata aaatttatag aaaaacacac gaatgatttt acaaaaaaga
     9541 tgccaacctt tttgtttgaa tcacccgtgt gctgttttta ttatcaatct atctttactt
     9601 atattgttcc tccaaacggc tcatccacca acctagtgct gtaccggcta taaaaagaac
     9661 aggacaagct agtaccccag caactcccaa tcccgcgtaa tcggtaggaa tgatcataat
     9721 cgttgaagca aaaactacac ctaaaatgaa atggaatagc ttgctatatg cctttgagaa
     9781 aatgtaatcc atgatttttg acaatccaag aacacaaaga atcccaccaa ttccgatcgg
     9841 cacaatcact tcaagtcgta cttctttaaa tccgtcagcc atcgctttat acaatcccat
     9901 atacaccaaa aaattagaag ggctcaatcc tgggacgatc atacccaaac caatcaatcc
     9961 gccggcaatc agccatgttc caaaattctg atcgacttgc ccgtcaaata attgagcgcc
    10021 gcataataaa aacaaataag ccagaacaaa agtaacaacc atgatgatca catcagaagt
    10081 atctcttcct tttttccctg cttctttcca taacgcagga acagttccag cgatacatcc
    10141 gacaaaaaac cataatatcg ttgtttcata cgcacctaat aaaaaactga ctaaaaaaga
    10201 aagtagaaag atgcctgtca ctccgcctag accaacaggc agaaaaaaca aaacattttc
    10261 tttgaaattt tttgtaaggt gggctaaaaa agaaatcagc cgctcataca tgccaaagac
    10321 agctgctaat gcaccaccgc tgactcctgg caagataaat cctgaaccga taaacattcc
    10381 cttcacaaac cgtaacatcc agtctttggt tgtgtacccc acttcttgat tttccatttt
    10441 ttgccttttt tccactattt gcgcactcct tctttcattt aattttattc atgattgaac
    10501 atttcctgct attttactta aagacttttt cataggtctc tattattttt tcattcattt
    10561 tgacaatatc aggataaaac atgttacctc cattatgata gagatagccg ccattgtaaa
    10621 gcaaggactg gatccgccaa tatcggtatt gtttcccttc ctcatttcct aaaagtgggc
    10681 tcagcacatc tttagaatac gcttctgcta attctactgt gttttctccg ccatgatttt
    10741 ttacataggc aatataatct ttcccaaaat tatatgcttg gacagctgtc cataaatcgc
    10801 atccttgttt ctttgcttct actatcattt ccgccaaata tttgacacct tgatcaatac
    10861 tgtcagctgg attatcaatg atctcagctt ctccatgcag actttccgag ctctgcatga
    10921 gatctgtccc ttttcctttt gattcggtca atattatcgc cttgatcaaa ttctcgtagt
    10981 tttccatatg ataatttttt gcagctgttg ttatttctat ttgatagctt tccactcgtt
    11041 ttaccgaacg ataagttctt cctcataaaa acaacaccgc accaataacc gtcagaatga
    11101 tcatgtaaaa aagaatacgg ataatagata atcgttttcg cttcatttca tcacctagtt
    11161 attttgacat ctttttttat cttactattt ctttatcttc ttggctattt cttccttgat
    11221 ttctgtgtac gccttaagaa aaaatgaatt ttcctgtatt ttttgacatc ttacgaaaat
    11281 gatcgttgtt tttcagtaaa aacaattgcc caatgactga tataatcgtt atactttaca
    11341 cagtaggtga ttatgtgaaa aaagaaaaga aatatgcaaa aatgccagat gggagcgaaa
    11401 tttactatga aaagagcggt caaggcttcc ctctcttttt actgcatggc aatgacggca
    11461 gcggccgctt tttttcagaa caggtccctg tactcgaacg ctactatacc gtgtatctag
    11521 tagatagccg aggacatggt cgttcgacaa acgaagcatc tatgttgaat tttcagttaa
    11581 tggccgagga tctgaatacg atcatgttgc tggaaaaaat cgatcaggca gattttcttg
    11641 gatttagtga tggagcaaat ctagcactgg tatttgccag cagcttccct aaaaaagttc
    11701 atcgattgat tttaaattct ggaaacacgc ttgttaaagg cgttcgtttt tctgccagag
    11761 tcatcagtaa tattcattat gcatgggtat ggttactctc tttatttcgc ccaagtcttc
    11821 gcaaaaatct tttagttatc aaacttttgc tgcatgacat cgggcttaca gaaaatgatt
    11881 taaaaaaaat caactccccc actttgatca tcgtgggaaa aaaagatgta atcaagctaa
    11941 aacattcttt gtatatagct aaaactatac caaaagcttc ctttgtgctt gtgaaagaac
    12001 aaggacatga attagctaga aaagatcctg aacgcttcaa tcgagaagtt cttcaatttc
    12061 tttctgaaac ataactatta ggaatagaag gtgatttatt tgttaaaaaa taccatacaa
    12121 tggctgaaaa accacctagg actgttcaaa acaatctttt tgatttctgt cattgtcatt
    12181 attgtcggtg aattgatgtc catcggtaaa actctatcta tccagcaatt aggagaaacg
    12241 tttgccacta tacctgtatg gaaaagcgga ctaatgctat tgattggtct tatctctgtg
    12301 ttacctatgg ttggttatga tattattttg aatcggatgc ttgaacaaaa gcagaatcct
    12361 cgttacctgt ttgagacaag ctggctgatc aatacgatca acaatattgc cggtttcggc
    12421 gggtttgtta gtgtggggct tcgctctgaa ctatatggac aaaagaaaga gagcagaaac
    12481 gtgatccaag cattatcgaa agttttcttg tttttgatgg ctggactttc tatttacagt
    12541 ctgctttctt ttttacttgt agtcttcacg ccagttgctc cttttttgaa gcaatattgg
    12601 atatggttga tcggcggcgg tctatatttt cctgtcgtct ttttatttac tactttcaaa
    12661 aagaaaggat tccttggtgg tacctcactt aaaactcaag gacagctatt gctagtttca
    12721 ttgttggaat ggaccggtgt cctgtttagt ttcctttctg ttggttattt aatggaaatc
    12781 cggattgatc tgtggcaaac aattccatta ttcattgctg cttcagtgat aggaatcgtg
    12841 tctatgattc caggagaaat tgggagtttc gatgtaatga tgattatcgg gttgtccgct
    12901 attggtgtgc caagagaaat agtcgttgtg tggatcttgc tctatcgcct gttctactac
    12961 atcattccgt ttttgattgg aatcgtcttt ttcttcaaaa atatcggttc cacttttgat
    13021 cagcgttatt ctggtatccc taaacaatta gcaacagaaa ttgcgcataa aatcgtggtt
    13081 gtcttattat acttttctgg aatcatgcta gtactttctg ccactatccc gcaagctttt
    13141 acagagtttc gctggcttca tagtttgaac ccattgaaat ttcattttat tatccaattt
    13201 cctagtatac tattgggatt tcttttaatc attatgggca gagggatcgc agcacgtgtg
    13261 aaacgagcat atttgcctac aatcttcttg attgttctag ctttacttta tgttttatta
    13321 agtgatttta gtttcacacc ggtggttttc ttgtctattc tgcttctgat cattcttgct
    13381 tctaaaaacg aattatttcg ggaacaattg atctattcat gggagtggcg tacgatcgat
    13441 ggaatcatta ttggtgcact gtccttgctt tacatcgtta ttggggtata caatctgccg
    13501 gatttccctc atcggcgtca ccattttatt tcctttttct tatttccttc tgaaaagatt
    13561 tggttttcgg gattgttggc catcatcgct gttagtttca tgatcgttct atttgttcat
    13621 ttccttcaag gagaaaagaa acagattggc gaagccttta atgaagaaaa ggccctgaag
    13681 atccttacaa cttatggagg aaactccgat agtcaattga tcttcttaaa agataagcgg
    13741 atgtttgcct atgaaaaaga cggtgaacca actgttcttc tccaattcgc ttgcttcaat
    13801 aataaatgta ttgtaatggg tgatccttct ggtaagaaag aagattttcc agaagcaatc
    13861 gaagctttta tcgaagaaac agatcgacta tgctatttac cagttttcta cgaaacaagc
    13921 gaagagattg ttatgatttt gcatgaattc ggctacgatt tcattaagat gggggaagaa
    13981 gcttatgtag acctgaatag ttttacaact tcagggaaaa aaatgaaggg cactcgtgct
    14041 gtcttgaatc ggatcgaacg agaaggattt acatttgatg tcttacaacc accattttct
    14101 actgagcaaa tgagtacttt caaaaatatc tctgataatt ggttgggttc acgaaaggaa
    14161 aaagggtttt ctttaggctt tttctctgaa gattatctac aaagagcgcc tattgcagtt
    14221 gtcaaaaata cccaagaaga aattgtcgct tttgcaacga tcatgcctac ttacacgaac
    14281 aataaagtag gaacgattga tctgatgcga tatgattcta aaaaagcacc ttctggcagt
    14341 atggatttct tgttcttgca tttgtttgcc tatatgaaag aagaacatat ccaatggttc
    14401 aatttaggga tggcccctct ttcaaatgtc ggaacttcca gaaagagttt tttacaggaa
    14461 aggattgcat acttggttta cgagtttggt tctcattttt attctttcca tggattaaga
    14521 gaatataaaa gcaagtacgc tacaaactgg gtctcacgtt atactctata ttctagagat
    14581 agttggattg catatgttat gattgctttg ctgattattg ataatgcgcc agttgagcag
    14641 acacaaggta gaataagtgg tttgaaaaaa tggatacgaa gaaagtatta aaaactgcat
    14701 accgatagat gaacactctc atgatgaata aaagagactg aagtaaaacg aaagttcatg
    14761 cttctacaat ctcttcttat acaattgaag aacaaagagc ggtccaaaca gaaatcctgt
    14821 ttggaccgct cttttatttt aaacattttt cattaccaaa atattcgagt cgcgtttctt
    14881 atgcttcgat ctctgtaacg atacctgaac ctactgtccg tccgccttca cgaattgaga
    14941 atgtcgttcc gttttcaaca gcgattggat ggatcaagtc aacatcaatc gtcacgttgt
    15001 caccaggcat gaccatttct gtatcttctg gcaatgtgat tgttcctgta acatcagttg
    15061 tgcggaagta gaactgtgga cgatagttgt tgaagaatgg agtatgacgt ccaccttctt
    15121 cttttgtcaa cacgtacact tctgctttga attttgtatg tggagtaatg gaacctggtt
    15181 tagcaagtac ttgtccacgt tcgatatcgt cacgttggat cccccgtaac aacacgccta
    15241 cgttatcccc agcttccccg taatctaacg ttttacggaa catttctaca cctgttacaa
    15301 ctgctttttg tgtttcaggt ttgatcccta cgatctctac ctcatcaccg acacgaacag
    15361 caccacgatc aatacgacct gaggcaacag ttcctcgacc tgtaattgag aagacgtctt
    15421 ccactggcaa tagtaatggt ttatctgtat cacgttctgg tgttgggata tattcatcta
    15481 ctgtatccat caattccata atagcagctt cagcatctgg atcgccttgc aatgctttta
    15541 atgcggaacc tttgatcaca ggagtgtcat cgcctgggaa accatattcg ctcaataact
    15601 cgcgaacttc catttctacc aaatcgatca attcttcatc atcgactaaa tcaactttgt
    15661 tcaagaaaac aatcaggtat tttacaccaa cttggcgtga caataaaatg tgttcacgtg
    15721 tttgaggcat cggaccgtca gttgcagaaa caactaagat tgcaccatcc atttgtgctg
    15781 cccctgtaat catgtttttc acatagtccg cgtgtcctgg tgcatcaata tgcgcgtagt
    15841 ggcgtttttc tgtttcatat tctacgtgtg cggtattgat cgtaatccca cgttcacgtt
    15901 cttctggagc tgcatcaata ctagcatagt cttgaggatt tgctaggcct tttttgccta
    15961 atactgtagt gatcgcagca gttaaagtag ttttcccatg gtcaacgtgt ccaatcgtac
    16021 caatgttaac gtgtggttta cttctgtcat aatgttcttt tgccataatt tacaaacctc
    16081 ctaaataata tagatcactg aaaaatttca gctctcacct ttttattata gtcttttttt
    16141 gactaaatca aaaagaatac gtccgactta ctttttgtaa gattcgaact aagcttatta
    16201 tacgtcaata ttggtcaaag tcaactaaga gcactcgtct gtcttatacg cgaaaaaagt
    16261 accttttata aataaaaaaa tgtgtttttt gagaaaaact tcaagatagt ccttaacttt
    16321 cttttgaagc aataagaaca aaatgaggaa aagtttaaaa ttcagctgtt gccaacaact
    16381 ttcaatagac gaaatcttct atttcgttaa taataaggaa agaggtgaag tacatgaaaa
    16441 aattaattgg cgtacttgtc gttttaggca tatttctcgg cggaagtttt gcagcttatc
    16501 attattttta cggcggcgaa gcttattata caaaaatcac gacgaatggc gaaatgtcag
    16561 ctacccaagc agataacgga gagaaattca cgacttatac ctacaagcaa gcggcttata
    16621 ataaagatgg tgaaaaaaaa caggtgacgc ttcgcgaaga aagagaacat ccattaaaga
    16681 tgaatgcgta tcttaaactc aaagtcaatc ctcgtaaagg tgtattaagc tgggaagaaa
    16741 taaaggcttc tgaagtgcct aaaaatgcgg cagaaaaaat cgataattag taaatggtct
    16801 taaaatttta ttagataaaa agaggctggg acagaagcgt ttaactccaa gaaataagaa
    16861 ggagttgctt ctgcttccgc cgtttatacg tgtttagagc gtgagacaaa agtgttcttt
    16921 acttttgtct cacgctcttt ttttgttata ttcgtctttt ccgatccttc tcttgacaca
    16981 attcatttaa ctgatcactg gtacttgaac acctaatatt ctagtcattt ttataaacga
    17041 ttcaatactt catccacatt tttgatcatc acggatatcg taccatccgg attattcaca
    17101 aattcaatca aatctgggtt gcgatataca tctactggta cgatcagttc gatcccgttt
    17161 gatagcttga atttttgttt tccaaatttt ttctcagaaa tttcttgtac ttcttttaca
    17221 ggaggtgctt ctgggacaaa tccttgctct tgtacttctt ctttaaaagc catttttgcc
    17281 gtgatgtttt ccttgaagac tttttccgca atcatctcat gatccaaccg acctttttct
    17341 tctatcgtat catagacagc ttctttcaaa tcagccatga tatcaaattc ttctgtctca
    17401 aatttttttc caagatgctt ggctactttt tggatcactt ttacattttc ttctaaagaa
    17461 ggtgcaggaa cactttcgat cactcttcca gaaaaataaa gctctttttt gccagaaaac
    17521 tcatattttt tttcaattaa ttcgtaggtc caatcagtca aattcacagc gaatgcttca
    17581 tctgcttttt gtgttttcgc ccctagaatt gctcgattca aaatcaagcg gttgtcgatc
    17641 cctgcttcgt ctgcttcaac aaagtgagtg taagcctcac gataattgac tttcaataaa
    17701 gataagtaca tcactgtatc taattcatac aagacaacaa atacatctgc actaggtgct
    17761 tcttcacttt cttggtagat atcgaaccac cgctgaacga ttgcttcact tgcttccaca
    17821 aaattttctt gagcgatttt aagtgattca acgatcatgc tttcttctgc tagttgtcca
    17881 gtttttgttt gagcagaaga gattttttgg atcttctttt gaagaaaatc acgaatatat
    17941 tcttttgtca gatccagctc tttttgtgaa aagactggag aaccagtttc tctgtcaata
    18001 atatgcagaa ttgctttttt taaatagata tccattttct cattcccttc tgtcttagtt
    18061 tacggaaaaa aaatcaaaaa taccagaatc aaaatcattt tcttttaaac aaaaatttca
    18121 agagtagaaa caaaatggat tctttttttc gaaaaatgcg ctacacttat agttgaacga
    18181 ataaaaaagg agctgaaagt atgaattcat taacaagcac ttatcgtcta tcaaatggtt
    18241 acgaaatccc ggtagtagga ttcggcacat ggcaaacacc tgatggggat gtcgctgttt
    18301 cctctgttaa agaagcgctg gctgcaggtt atcgccatat cgatactgcc caaggataca
    18361 agaatgaaga aagcgtcggc caagcgatta aggaaagtgg aattcctaga gaagagatat
    18421 tcttgacaac aaaattatgg aatgcaaacc acagctatga acttgtgatg tcttcttttg
    18481 aagaatcatt gaagaagctc caaacagatt atttagatct gtttttgatc cactggccaa
    18541 accctgttgc ttttcgtgat aattgggaac aagcaaatgc agatacatgg cgagcatttg
    18601 aagaactgta tgaagcaggt aaaatcaaag caatcggtgt gagcaatttc ttaccccacc
    18661 atctagatac actagcaaaa acagcaaaaa ttatgccgat ggtcaatcaa gttttccttg
    18721 caccaggcga attacagaca actgtagttg agtatgcaaa aaaacatgat atgatcctcg
    18781 aagcatatag tccattaggc actggtaaaa tcttcgatgt gcctgaaatg aaacaaatcg
    18841 ctgaagcaca tgacaagacg attgcacaag ttgctttgcg ttggtcgctg caacacgaat
    18901 tccttcctct tcctaagtca gtgacaccaa gcagaatcaa agaaaatact gaattatttg
    18961 attttgaatt aacagaagaa gaaatgaaac aaatcgatca gttagatggt gtagtcggca
    19021 aagcaaaaga cccagatact acccaatttt aagtcgaaag gtgattttat ggatccaatc
    19081 gaacgcttga attctctctc ggaagaagtt acccagactt ttcattccga ttttgtcttt
    19141 ttgatcgatg cagagaaaat ccaacatttt cctgcaagaa actggacaca tgatcaaatc
    19201 atcgaagaac tgaaaaaacg gttcgatcat tcgctaatgg tcaaaccctg gcacgaacat
    19261 gaagtcattt attcacctga actacctgtt tttgccttga taccgaaaaa atagaaa
//
LOCUS       DACDUU010000060        18974 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.060, whole genome shotgun sequence.
ACCESSION   DACDUU010000060 DACDUU010000000
VERSION     DACDUU010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.060"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(90..1388)
                     /gene="eno"
                     /locus_tag="IWL30_002065"
     CDS             complement(90..1388)
                     /gene="eno"
                     /locus_tag="IWL30_002065"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAP9706804.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(1504..2259)
                     /locus_tag="IWL30_002066"
     CDS             complement(1504..2259)
                     /locus_tag="IWL30_002066"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAP9706805.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(2363..3553)
                     /locus_tag="IWL30_002067"
     CDS             complement(2363..3553)
                     /locus_tag="IWL30_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAP9706806.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(3663..4664)
                     /gene="gap"
                     /locus_tag="IWL30_002068"
     CDS             complement(3663..4664)
                     /gene="gap"
                     /locus_tag="IWL30_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP9706807.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(4705..5742)
                     /locus_tag="IWL30_002069"
     CDS             complement(4705..5742)
                     /locus_tag="IWL30_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAP9706808.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            complement(5945..7279)
                     /gene="rpoN"
                     /locus_tag="IWL30_002070"
     CDS             complement(5945..7279)
                     /gene="rpoN"
                     /locus_tag="IWL30_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAP9706809.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            7339..7410
                     /locus_tag="IWL30_002071"
     tRNA            7339..7410
                     /locus_tag="IWL30_002071"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7372..7374,aa:Arg,seq:ccg)
     gene            complement(7602..8486)
                     /locus_tag="IWL30_002072"
     CDS             complement(7602..8486)
                     /locus_tag="IWL30_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP9706810.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(8573..9472)
                     /locus_tag="IWL30_002073"
     CDS             complement(8573..9472)
                     /locus_tag="IWL30_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAP9706811.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            complement(9489..9995)
                     /locus_tag="IWL30_002074"
     CDS             complement(9489..9995)
                     /locus_tag="IWL30_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAP9706812.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            complement(10184..10804)
                     /locus_tag="IWL30_002075"
     CDS             complement(10184..10804)
                     /locus_tag="IWL30_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAP9706813.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            complement(10880..11317)
                     /locus_tag="IWL30_002076"
     CDS             complement(10880..11317)
                     /locus_tag="IWL30_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAP9706814.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            11480..12310
                     /locus_tag="IWL30_002077"
     CDS             11480..12310
                     /locus_tag="IWL30_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAP9706815.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            12294..13661
                     /locus_tag="IWL30_002078"
     CDS             12294..13661
                     /locus_tag="IWL30_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9706816.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            13680..14489
                     /gene="yidA"
                     /locus_tag="IWL30_002079"
     CDS             13680..14489
                     /gene="yidA"
                     /locus_tag="IWL30_002079"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAP9706817.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(14532..14975)
                     /locus_tag="IWL30_002080"
     CDS             complement(14532..14975)
                     /locus_tag="IWL30_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP9706818.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            15109..15480
                     /locus_tag="IWL30_002081"
     CDS             15109..15480
                     /locus_tag="IWL30_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP9706819.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            complement(15649..16431)
                     /locus_tag="IWL30_002082"
     CDS             complement(15649..16431)
                     /locus_tag="IWL30_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAP9706820.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(16428..17399)
                     /locus_tag="IWL30_002083"
     CDS             complement(16428..17399)
                     /locus_tag="IWL30_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP9706821.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            17491..18885
                     /locus_tag="IWL30_002084"
     CDS             17491..18885
                     /locus_tag="IWL30_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP9706822.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
BASE COUNT         5359 a         3901 c         3379 g         6335 t
ORIGIN      
        1 ttcagcaagt agagagttcg attacttaac taagtgatca tactcgacta tctttcaata
       61 gtcgatcatg tatcttcatt agaaaagaat tatttgtttt ttaagttgta gaaagatttc
      121 aaacctttgt attctgcaac ttcaccaagt tggtcttcga tacgtagtaa ttggttgtat
      181 ttagcaatac ggtcagtacg tgatagagaa ccagttttga tttggccagc atttgttgca
      241 acagcgatat cagagattgt tgaatcttct gtttcaccag aacggtgaga tacaactgct
      301 gtgtagccag cttctttagc catttcgata gcttcgaaag tttctgttaa tgtaccgatt
      361 tggttcactt tgataaggat tgagttagca attccttttt cgataccttc agataatttt
      421 tgagtgtttg ttacgaacaa gtcgtcacca actaattgaa ctttgtcgcc taatacgtca
      481 gttaatttct tgaatccgtc ccagtcgttt tcgtctaatc catcttcgat tgagatgatt
      541 ggatatttag aaactaattc ttcgtagaat ttgatcattt cgtcagttgt tttttcgcct
      601 tcgcctgagt cagctagtac gtaaacacct ttttctttgt cgtagaattc tgaagaagca
      661 gcatccatag caagaacaac gtctttacca ggtacatagc cagctttttc gattgcttcg
      721 atgattactt caaaaccttc ttcgtttgaa ccaaggtttg gagcaaatcc accttcgtca
      781 ccaacagaag ttgctaatcc gcgagctttc aagattgaag ctaatgcgtg gaatacttca
      841 gcacccatac gtagagcttc tttgaatgtt ggagcgccaa caggcatgat catgaattct
      901 tggaagtcga ttgagttatc agcatgtgat ccgccgttga tgatgttcat cattggtgtt
      961 ggcaatactt ttgtgttgaa tccgcctagg tagtggtata aaggtacttc taggtagtca
     1021 gcagctgcac gtgcaacagc gatagaaaca cctaaaatag cgttcgcacc taatttacct
     1081 ttgttaggag ttccgtctaa agcgatcata gctttgtcga tagccatttg gtcacgaaca
     1141 tcgtagccaa tgattgcttc agcaatgatg ttgtttacgt tgtcgacagc tttagttaca
     1201 cctttaccgc cataacgagc tttgtctccg tcacgtaatt caactgcttc gtattcacca
     1261 gttgaagcac ctgaaggaac cattccgcgg ccaaaagctc cgctttctgt gtatacttct
     1321 acttcgattg ttgggttacc acgtgagtct aagatctcgc gagcgtaaac atcagtaata
     1381 attgacatgt tttgtctctc ctttgagttt gttttttcct aagggatttc cctcatatac
     1441 gattttagtt aattttgctc attagtgcaa acatttcttg catttttcgc aaattttcaa
     1501 caattatttt acagcgtcta acaatgacaa aaatgaatct gcttgcaagc ttgcgccacc
     1561 gactaaagca ccgtcaacat tttcttttgc catgtattca gcaatatttt ctggttttac
     1621 agaaccgccg tattggatac gaactgcttc tgatacttct tttccataaa gtttttcaac
     1681 tgtttgacgt acaactccac aaatttcgtc agcgatttgt gcgtcagctg atttaccagt
     1741 accgattgcc cagattggtt cataagcaat aaccatgttt gacacttgtt cagctgaaag
     1801 atcagctaaa ccagctgtaa tttgtccttc gatccattca gcagttttgc ctgcttcata
     1861 ggtttctaaa gattcgccgc agcataggat tggtgtcata ccatttgcaa agatagcttt
     1921 tgctttttta ttgatgtctt cgtctgtttc atggaaatat tcacgacgtt ctgaatgtcc
     1981 gatgatcaca taatgaacac ctagatcagc aagagctgct ggtgaagttt caccagtgaa
     2041 cgcacctgag ttttcccagt agcagttttg tgctgagatt tttagttctg taccttcagc
     2101 tgatgtcatt aattcttgta agaataatgc aggagatccg atgactgaat caactttgtc
     2161 atttgaaggg attttgtttt ttactgcttc agcaaattct tttgcttctg atgcagtttt
     2221 gttcattttc cagttacctg cgataatagg tttacgcatt gaggaacaca ctccttaatt
     2281 tattgaaaaa tggctaggga gtcatgaaaa ctcccttcac cttttgatcg attactcgtc
     2341 agaattgagc aaacttggtc gtttatttat cgttgattgc tgctagtcct ggtaattctt
     2401 taccttcaag caattccaag cttgcgccac cacctgttga gatgtgtgtg aatttgtcag
     2461 caaatcctaa ttgttctgct gctgcagctg agtctccgcc accgatgatt gttgttgcgc
     2521 cttctaagtt agcgattgct tcacaaacac cgattgtacc tttagcaaag ttactcattt
     2581 caaatacacc cataggtccg ttccaaacaa ctgtttttgc accttgtaat gtatcagcaa
     2641 ataattttac tgatgctggg ccgatgtcta aagccatcaa accatcagga atgtctcctt
     2701 cgattacttg tgtttcaaca tcatttgaaa attctggtgc acagacattg tcgattggca
     2761 agacaagttt gtcgcctgct ttttcgatta attcttttgc tagttccact ttatcagctt
     2821 ctactagaga gttaccgatc ttgattcctt ttgcttcgta gaatgtgtaa gtcattccgc
     2881 caccgatcaa gattttatct gctttaggaa tcaagttttc gattacgccg attttatcag
     2941 aaacttttgc gccaccaagg attgcaatca ttggtcgttt tggttcttct actgcttcac
     3001 cgatgaactt gatttctttt tccatcaaga atccagcaac tgttgggata ccagttgaag
     3061 cgatacctac gttagaagca tgtgcacggt gagctgtacc gaaagcatcg ttaacaaata
     3121 catcgcctaa tgaagcccag tatttaccta gttctgtatc atttttgctt tcttttttac
     3181 cgtcaatatc ttcaaatcgt gtattttcga atacgacaac gtcgccgtca ttcatgttgc
     3241 ggattgcttc ttctaattgt tctccgcgag tttcaggtac gaatgttact tctttaccta
     3301 ataattcgcc tagacgtttt gcaactggag ctaaagattt tccttcttta tcttcttctg
     3361 tttttacacg tccaaggtga gagaaaagga ttgcttttcc gccttgttct agaacgtagt
     3421 tgattgttgg caatgctgct ttgatacgag tgtcgtcagt gatcacgccg tctttcaaag
     3481 gaacgttgaa gtcaacacgg acaagaactt ttttgtcttt taaatcgata tctttcactg
     3541 ttttcttagc cattttattt cctcctgaat aaacttatta tcaaaaaaag cggaagaagc
     3601 gcgattgctt ccccgctttt aaatgtacat gtctataatt tacacttgta cgtttgtcta
     3661 tcttataagt tagcgaagta ttctaaagta cgtactaatt gtgcagtgta tgacatttcg
     3721 ttgtcgtacc aagcaacagt tttaactaat tgtttgtcgc caactgtcat tactttagtt
     3781 tgtgtagcat caaataatga accgtaagtc ataccaacga tatctgaaga aacgatttcg
     3841 tctgtgttgt atccgtaaga ttcgtttgaa gcttctttca ttgctgcatt gatttcgtca
     3901 acagttactt ctttttcaag aactgttact aattctgtta atgaacctgt tggtacagga
     3961 acacgttgag cagctccgtc taatttaccg ttcaattcag gaattactaa accgatagct
     4021 ttagcagcac ctgttgagtt aggaacgatg tttgcagcag cagcgcgtgc acggcggaag
     4081 tcacctttag gatgaggtcc gtctagagtc atttggtcac ctgtgtaagc gtggatagtt
     4141 gtcattaatc cttcaacaac accaaatttg tcgttcaatg ttttagccat aggagctaaa
     4201 cagttagtag tacatgaagc acctgagata actgtttctt taccagttaa tgtttcgtgg
     4261 tttgtgttat aaacgattgt tggaacatcg tttccgccag gagcagagat aacaacgcgt
     4321 ttagcaccag ctgttaaatg tttttcagct gctgatttag aagtgaagaa accagtacat
     4381 tctagaacga tatctacgcc tagttcgccc catggtaatt cttctgggtt gcggttagct
     4441 aatactttaa cttcttttcc gtttacgttg aatgaacctt catgaacttc aactgttccg
     4501 ttgaaacgtc cttgagttgt gtcgtatttt aacaagtgtg ccaacatttt agcatctgtc
     4561 aagtcgttga ttgctactac ttcgattcca tctacatctt ggatacgacg gaatgctaga
     4621 cgtccaatac gtccaaaacc attaatacct actttaactg tcatttaaga tttcctcctt
     4681 atgaaaatca aaaaaatatt tattttaaag ggttacccct tttaaaatct catttgcagc
     4741 agcttcatca gtgatgagcc acgtttgttt tggtgcattt ttcatataag ctcgaattgc
     4801 tttagctttt gattttccgc ccgcgattgc catgatgatc gggacttttt ccaaatcttg
     4861 caattctatg ccgattctag ggaccttata aacgacttca cctttttcat tgaaaaagta
     4921 gccaaaagat tccgcaacag catttgcttg tttcagcatg actaattctt tttcagtcat
     4981 cttacgacgt gctgccatgt gtaatgcacg cccaatacta tgaataacac aattcgcttc
     5041 actaatcaag tttaagactt cttggataga tggctcattg agtaaagaat tgtaagttgc
     5101 cagacttaat tgttccggaa cataaagtgc acggtggtta ccgtttgctt tagcagccat
     5161 tcttgcactg acagaatttg cctgtacagt tatcgcttca ccaatcccgc ctcttgcagg
     5221 gacaaacagg ttatgccgtt tcttattttc cagattagac aattgttcgg ctaccacagc
     5281 catagtagtt cctcccatca cagcgatgat attctcgcct tctggaagta gtcggtctag
     5341 ggcttcgctc agcgcttcgc cgaatgcatc agccactttg gtctgctctt cgctgtttcc
     5401 tgaaacaacg atacagcgtt ggttaccaaa atattctgcc aactgttgtt cggtttgatg
     5461 cgtacctaaa agctgatcca tgaagttttc aaggctttga taaacttctt cgcccttagt
     5521 tgtcagcgtc ataccgcttt ttgaggtgtc gatcaaattc agatttttca aaagatctgt
     5581 ttctgttcgc aatactcgtt ctgtgatccc catactttcc gataaacttc gacgtccaat
     5641 cggctgcatc caatagatat ttcgtaaaat tttatagcgt tcttgcaaca cttcgataat
     5701 atctggtgca acagattcaa tcatttttat ttcatccagc atagcgatct ccttagttgg
     5761 acactttacg tccaatgtag acttaaaacg acccatatac gataaaaaaa gaaattaact
     5821 attcgaattt cacatcaata aagggaaatc tcaacctcac gactgtaagt ctaacagttt
     5881 tgctctcgtt tttcaactaa aaagactaga aaaagtgaaa tactttttct agtcttttga
     5941 ctgattagtt atcataacgt tttctttttg atgaactcgg gattcctaat aaatcacgat
     6001 attttgctac tgttcgtctt gagatcgcta tctcatcttt tttcagcaat tcaaccaatt
     6061 tctggtcaga caacggcttg tttttgtctt cttgatcgac taattcctgc agctttttct
     6121 tcgctgtatc tgctgaaagg tcttctgtct gttctgtact attcgtagga attcgtgtag
     6181 tgaaaaattt ttttaattca aatacaccga agtctgtttc caaatatttc ccgttgactg
     6241 cacgactgac cgtggattca tgcacatcta tctcagttgc aacttccttt aacgtaagag
     6301 gcttgattgg acgttccttg tcgataaaga aagctttttg ccgggaaaca atgacttgag
     6361 caacccgcaa aattgtatcc ccacgttgta ggatcgtttt cttcaaccat tcgaattcct
     6421 gtttccgttc ttttaaataa ttttcagttt ctttatctgc ttgttgtgac atttgtttga
     6481 agtaactctc ttgaaagcgt atatttggct gattttttcg gtttgagata acttgtacat
     6541 ttttatcgtc gtccactaat acccggacat caggaataat gtacaattcg ctgctacggt
     6601 cgaaaatccg gccagggctt ggactcaacg tttgtaaata gtcgaatatc tgctgaacag
     6661 catgtagatc aacagaaaaa cgttgtgcga tttctttcca ttttcgctcc accagttcct
     6721 caaaacattc ttccaacacg atataagcta attccggcgc ataatcatct cgttctgttt
     6781 gaagcatgag acattcttgc aaactacgtg cacctactcc agctggttct aattgctgaa
     6841 tcagagtcaa cgcatcgagc atttggatag gcgtagctcc aatctctttt ccggcttctt
     6901 ctaaactaat cgtcaaaaat ccgttcaaat cgatgtattc tacaagataa agaacgattt
     6961 tcctcaaaaa agtatctcgg taattcaaat ggacttgttc gatcaatgaa tcaaataaag
     7021 acccttttgt atcaggaatc tgatttaggt aatttgtctc ttctccttca taagaagatg
     7081 aagaagcttt tatgtaatct ggctgccatt ccggctcaac aacttccacc aatggatttt
     7141 ccattgcttg attctcaacg aatgccaaca gctcctcact gttatattgt aaaacttgga
     7201 tggactgttg caactgttgc gtcatggcta gtttctgtgt ctgctgctgt ttttgtgaaa
     7261 aattttgctc aaatttcatt tttttgtcca atccccttgt aattttttat ctgtttagtt
     7321 acaattatcc cagtgcttgc gtccttagtg tagtggatat cacgtaagat tccggttctt
     7381 gagacggggg ttcgattccc tcaggacgcg ttctactgct attttagcat aatttacgaa
     7441 tagttgcttc tttattttta agtgaaaact gtccgccaaa gaaaattggt ggacagtttt
     7501 ttgttgtacc tgtatagata ttgttaagcc tttgcaagta aggtagttat gtgttcaact
     7561 acttattaaa atgtgttatt tattaaagat acttgtctca cttactgaac tggccgatga
     7621 tcgtttctgc cataatagtt ctcgacagcc gttggatgcc ctaataaatc cccaactgtc
     7681 gtgatgcgat acccttgctc tttcagataa tcgatcactt gaggaacggc acgaatcgtt
     7741 tcaggataga tatcatggaa taaaataatt gccccatcgt atactgttgc ttggatcttc
     7801 tgcaaaatca aatcaggatt tcttgtcttc caatcttctg aatcaacaga ccattcgatg
     7861 atcggtcgct ggatgatcgt tgctcctaca ttcgtgacag aaccataggg cggacgaaca
     7921 aaagtggggg ttttgccagt gacttttttg atctcagtgt ctgctttcgt cacttcctcc
     7981 gtcatcgact gggcagaaag agtggccaga ttctgatgat cgtatgtgtg attgccaatc
     8041 tcgtgccctg cttcagctgt ctgcttcacg atttcaggat gctgcgccgt attttctcct
     8101 aagacgaaaa aagtggcatg cacgtttttt tcttttaaga ttcgcagaag ctgcggtgtt
     8161 gtggtcgctc ctggtccatc atcaaagctg attgcgacgc ttttgacagc cggatctcgg
     8221 ctcgtttttg tactgtcttg tgttgattgt gatgactgac tttgtacctt ttccgctgat
     8281 tcttgaatgg cttctttgac gttagctgtt tcagttgatt ggcttttaat ggttgattgt
     8341 tctgattgtt tgaaagattg tgacgaatgc tccgaagagt gtgtttgtcc ttttacttcg
     8401 gtgatcaagg aggccaccga agtaatttcg aaaaacaaaa gcacggataa agcaataata
     8461 ctgataacta aataatttct tttcactata tctcttcttt ctctttttct cttcttatta
     8521 tttacttgta cttacttgaa aattgaaact gctagtcgca actacttctc gtctaatgat
     8581 ccaaataatc ttccgatgcc aaaatcgttt ttgtcacgga cttaggaatg acaaattctg
     8641 gtgtacccat ataaccagga gcaatttcgt attgagaaaa agtcaaaact aattgatgct
     8701 ctttgttcaa ataaaaactt cgtgtaggcg taacaagtgt tggtttttct atcgtccctt
     8761 cttgaatatc ttgctcagtc caatagtact tagatggatc ttccttgact tgttcggcca
     8821 tctgccgttt gacttctttt gtcaaaacag atagataggt gtcatctttg aataaaagtg
     8881 gaagtgagag ctggacactg tttttcttgt cgattgtgtc gtatcttttt tcaacatggc
     8941 tatctgctgc tgtttgggtg atctgccttt caatcacgag aaaccggtta tcgtctacga
     9001 ttttctgata atctcccaaa acttggtaat gatccgtttc aaaatttcca tattcttttt
     9061 cagctttttc aaaatctgct tgtccttcgc gaaagtattt ttgatttaat ccatgaattg
     9121 ttttattttc tttctcattt tgtgtattga tcttcggtac ctgaatatcg attttttcag
     9181 atcgatcagt aaagcgttcg cctaagatca attcaatgac tgatcctaag atgggtaaat
     9241 cttctgcaaa gcttcgaact tgactattaa aaagaaggct acttgttgta atcattacga
     9301 tagccaaaga tactccgaac ccttggagac gtctctttct ccgctttgct tttataaatg
     9361 attgctcttc gataataaaa cgctggcgga tctcctcttt aagccctttg ggaattgctt
     9421 gttctttata agttttcttt agttttttta aattttcttt ttgggaatac atggcactct
     9481 tctctccttc attctaggat ttgacgtaaa gcattcaatg agcggtacat tttcgtttta
     9541 actgtgtttt cattcatatc caagatagtc gcaatttctt tcaaacgaaa tccttcaaaa
     9601 aagtagagga tcagaatctc tttttccgga ctgtctattt tatctaaaat catatggagc
     9661 tctgcaacgg aagtttctaa aggtaactgt tgggggatat cttgaatttc aaataagtcc
     9721 acaggcgtcc ttttttgtct gcgccaacta tctaatgccg tgcggatcaa tatccgataa
     9781 aaccatgcac taaaatgctc gatctctttt tctttttcaa aatagttcag ggctttatga
     9841 aaactatctt gcatcacatc taaggcctct tctttatcat gtacgtaatt catcgtgata
     9901 cggaacattt tttgataatc agtttcgatg agtccattca gcaggtgacg gtatttttcg
     9961 atctgccgtt ttttcttata tccagtaaaa atcattctcc actcctttct gcctcactta
    10021 atatgacgta caaggtgcgc taaaagtttc aattttttta ttttttatgt atttacttgg
    10081 gatttttata tacaaaaaag aaggtgtgac aaaaatcgtg tcacacctcc ttattctgat
    10141 aggctgatcg tctctgtcac aacctctcgt aagaaaataa tgtttattct ttttcagagt
    10201 cagaaaattc ttcctcttca taatcttcat catcatcgtc ttcgtctatg atacttaaat
    10261 cttcttcgat atttcctggc aagtcttctt catcatctgt cgggtcatct gctccgattt
    10321 cttgcaagtc agaattataa gcagcgattt cttcatcttc atcatcatcg tcatcaaaca
    10381 gatcatcgtc atcttcttct gtcagatctg tatcttctgg gtcatcgtca ttgtaatcga
    10441 tcgcgtcttc atcatcattg ataaaggcgt tgactttctt gcgtttacgg cgacgtggag
    10501 tatcttcatc ctcttcgtcc attccatggt ttacttcttc atcgatcgaa tcgatagcat
    10561 accatgaacg caagccccaa cgattatccc ctaatgagat aaagctgccg tcaatattca
    10621 agtcagtata aaattgtgcg agtgaatcac gaatttcact gtctgatttc ccaagatagt
    10681 tttggatctg gttagctaaa tcagaaaaat ccatgacatc gccacgttgt tctaagatcg
    10741 cgtgtgctac ttcgatcatt gataattcgc ttttgtttgc accttcaaat acactaattt
    10801 ccaatcagga cacgtccttt cacagtctac tccttatcat acaaaatcag acacaaaaaa
    10861 tcaaactttt cttttatatt tacgctgtaa aatccaaggt caaataatat tcgccgattt
    10921 tttccggtcc catgaacaga tcataatcga ttcgaacctt ccctgctgtg ggacggtctt
    10981 tcaaactaac atgcagttct ttcgtaaaag tagcaatctg gatcataccg tatggtgttc
    11041 gataggatgt ttctaaccgt tctttatagc caaatttcaa acgcatccgc atttctcctg
    11101 cacggatcag ctgtacatga ccatctggaa ctaatttgat cgttacagga atctcatcgc
    11161 ccgtctcctc ctgaatctct ttataacgta tgtacagcgt atcgccaatt gtcaccattt
    11221 gccctttcaa atcaaaataa aaatcttgca cttccccttg ctgttttact tttgtacgca
    11281 atttgattga aatcggtgtt ccttgtgata attccattat tcattctcct ttacttctca
    11341 tagctacatt gttcctagtt tcttttgttt tgtcaaccaa cttgtccatt tttacagaaa
    11401 accttttttc caaaacaact ctttgatttt agagtataat attgtagaaa atatgaattt
    11461 atacaaagga gccggttgaa tgaaccctgc gattcgacta gatcagcaca tttatcaaaa
    11521 aaacgacttt tccccttttg cattaacaga tatattaacg gaatattcta aagaacacca
    11581 gcaaatcttt ttacatttct ggcaatatga ctgcacagtc atcttaggta tgaaggatgt
    11641 tcgaactcct tttctaagtg acgggatcca aacactggaa gatgcaggtt acgctccagt
    11701 gatcagaaat tcaggtggat tagggatcgt atcagatgaa ggaattttga atatttcttt
    11761 gattttcccc caaagtgaca caaaaaaaat aaccatagat gacgcttacg agcaaatgct
    11821 cctattgact aaatacgctt ttccaaaggc accgatacaa gcattcgaaa tcacccattc
    11881 ttattgtcct ggaacttatg atttaagcat ccatggcaaa aaatttgccg gtatcgctca
    11941 gcgtcggatc aaaaatggga tttctgttat gatgtactta agtgtcaacg gtaatcagga
    12001 aaaacgtggg caagtcatga agcgatttta tcaatctgct ttaaaagaag actttggtac
    12061 aaatggctat cctgcagtgg atcctgctac aatggcaaca cttgaagaac tgcttgacca
    12121 accccttttt gtaaaagaag tcaaagaagc atttgcaaaa gcttttgaaa agctgtattc
    12181 tgcaagtgat ttacaggatt ctaccgactg gctaaaaaaa gaaatccagc tggagcaatg
    12241 gaacaaccaa atccagagaa tggaacaacg taataacata aaggagctac attatgacaa
    12301 ccccttataa atatcaaatg ctgcctatgg aaaaagtttt tcgtgatcca gtccataact
    12361 atatccatgt ccagcatcaa gtaatcttag atttgatcaa ttcgaaagaa gtccaacgtc
    12421 tacgacgcat taaacaatta ggcacttctt ctttcacttt tcacggagca gaacatagcc
    12481 gttttactca ttcattaggc gtttatgaga tttcaagacg aatctgcgat atcttttctc
    12541 gaaatttttc aaaagaaaaa ataggcaacg gaggctggga tgatcaggaa cggctggtga
    12601 ctttatgtgc ggcactgttg catgatgtcg gtcatggtcc ttactctcat acatttgaac
    12661 atatcttcca tacgaatcat gaagccatca ctgtagagat catcacttct ccagaaacag
    12721 aagtgtatca gattcttaac cgagtagaag aaggttttcc tgaaaaggta gctagtgtga
    12781 tccaaaaaac ttaccccaac ccacaagttg tccaaatgat ttctagtcag atcgatgcag
    12841 atcgaatgga ctatttgctt cgggatgctt atttcactgg aacagaatat ggaacatttg
    12901 atcttacccg aatattacgt gtgattcgtc cttatgaagg cgggatcgct ttttccatga
    12961 gtggcatgca tgcagtggaa gattatatcg ttagccgata tcaaatgtat gtgcaagtgt
    13021 acttccatcc tgtttcccgt ggtatggaag taattttgga gcatctgctt catcgcgcac
    13081 acgagttgta tcaagaagat gccagcagtt ttgaactcca ttctcaatta cttctgccat
    13141 ttcttgacgg aagctttact ttagaagaat acctcaaact tgatgatggg gtcttgggca
    13201 cttactttac tcaatggtct gaatctgctg atcctatttt aaatgattta gctaaacgtt
    13261 ttttgaatcg taaaccactg aaatctgcta ctttttctgg caaccgggat tcaaaattag
    13321 tccaagaact cactttgtta gtagaaaagg tcggatataa tcctgtttat tacactgctg
    13381 tgaattctag ctacgatcta ccttatgatt tttatcgccc agaacaaggc agacatcgca
    13441 cacaaatcga gattttgcga aatgacggaa ctttgataga attatcacag gttagtcagc
    13501 ttgttgctgc attagcggga caagaacaag gagatgagcg atttttcttt ccaaaagaaa
    13561 tggttgatcc gtccttgcgt gatcattatg atttatttga cgaaacttat caagaatttg
    13621 ccagccatat ccgtaatggc gcattgattg aaataaatta aaatatattg gaggaaatta
    13681 tgtctatcaa actagtcgct atcgacatcg atggtacttt attaaatgag aaaagagaag
    13741 taacaaaaga agtaaaagaa gcgattgccg ctgctgttgc aaaaggtgtt tcgatcgttt
    13801 tatgcactgg ccgtccctta ccaggtgtcc aggaacagtt aaacgagttg aacctttttc
    13861 aagataatga ttacgttatt acttataacg gggcactcgt acaacaaacg aaatcaggta
    13921 aaatcatcgc ccgtcacgga ctgacacatg aagatttcct agagatcgaa gtaatggcac
    13981 gccgtgtcgg cagtcatctc cattcgatcg atgacaaagc gatctacacg cctaatcgag
    14041 atatcagcgc ttatacgatc cacgaagcaa gtttagtcaa gatgccgcta aaatatcgaa
    14101 cacctgaaga aatgacacca gacatgaaca tcgtcaaaat gatgatgatc gacgaaccag
    14161 aaattttaga tgcagcaatt gcgcgtttac cgcaaacttt ccgtgataaa tacacaacgg
    14221 tcaaaagtgc gccctattac ttcgaagtac taaataaaga agcaagtaaa ggtgcggcag
    14281 ttgcgaatct cgcacagcac cttggtattg atcaagatga aatcatggct atcggagata
    14341 acgagaacga cttatccatg attgaatatg ctggtctagg agtagcaatg ggaaatgccg
    14401 tttctcttgt caaagaagca gcaaatgtca tcacttcatc aaacgacgag cacggtgtag
    14461 ctgaagcaat caaaaaatat gtactgtaat aaataaaaaa actgagagaa agctgatcta
    14521 ctcgctttct ttcagttttt atttttttcg atccatctct cgactgccca tcggtggcta
    14581 ccacgtttca atttcacaag cgcttcttga gggctcaccc accaaatttt attggtcttt
    14641 tcagttggtt ctcctatacg cttccaagaa tcagcaacaa agaaatatcc tggattataa
    14701 taataagttg cacgataatt cgagtaaaaa tactcatcag cttctcccag ataagaagaa
    14761 atttctgcca caattcccat ttcttctatc agctcacgga caatcgcttc ttctttcgtc
    14821 tcttctcctt caatttcccc gccaggaaga aaaaaagcac catttggagc ctcgattact
    14881 gcaatctgct gatctctttc tatgacaata tatgctgcat aacgggtttg ataatcagca
    14941 aattgctttt tttcaccaaa aattggttta tccatgaata gtttccacct taacagtttt
    15001 attttctatc cattttacat gagaaatttc tcatgaacaa gcacaaatca actatttcct
    15061 tacttctttt tgttatgatt gagtaaaagg aaaaggagag atgaatctat gaaaatggct
    15121 cacacatgtg ttcgagtgaa agatcttgaa gcatcaatcg aattttatca aaaggcattt
    15181 ggttttgaag aaagccgccg ccgtgatttt ccagaaaaca aatttacact tgtttatttg
    15241 acacttcctg gggatgacta tgaattagag ttgacttaca actatgatca tggtgcgtat
    15301 gatctaggag acggctatgg tcatatcgct atagcagctg atgatctaga aaaattacac
    15361 gagcaacata aagctgctgg tcttgagatt actgacttaa aaggattacc tggcactgcg
    15421 ccttcttact actttgtcat tgaccctgat ggttataaaa tcgaagtcat ccgaggatag
    15481 ttagtatggg gcattttact cttgattgaa taaaaaacaa acaccaataa ttaaaatgag
    15541 acgcaaactt ttcattaaat tgaaaaagtt tgcgtctttt tttaacaacc attgtagcta
    15601 taaggttgcc actctccacc aatttatttt taattcattt gaaagaaatc aaataaacga
    15661 ggtaccttta gaaaacaaaa ctgcttttcc tcctattatc acacgatctt cttttaattc
    15721 acattctaga tccccgcccc ttgatgacat ttgataagct gacaattttg ttttattcat
    15781 tctttctgcc caataaggaa taagattaga atgtgctgat ccacatactg gatcttcatt
    15841 gattgttaat tttggaaaga aggttctaga aacaaagtct ttttgcacac tttctgctgt
    15901 cacgatgact cctattccta attcaaaatt tttgatagct gtaaaatctg gctgcaattt
    15961 agccaccgtt ttttcatctt ctaatacgaa aaataaatca cgtgctaagt aggcttcttt
    16021 aatcttagcc ccaatcgctt cttcatattc agacaatatc ggcacttttt tcggcataat
    16081 actaggaaaa tccatgtaat aataatcttt aattcttgtc acaaatagat taccgctttg
    16141 agtagaaaat ttaatcgttt catcaggtat tttgtaatag ttaaataaaa caaatgctgt
    16201 tgctaatgtc gcgtgaccac aaagatcgat ttctcgatct ggcgtaaacc atctaagctc
    16261 aaattcttgg tttttcttca ctgtaaatgc tgtttcagat aaattgtttt caatggcaat
    16321 tttttgcatc gtaccttctg gcagccattc ttctagcaca taaaccgctg ctggatttcc
    16381 tttgaacacc tcatctgcaa atgcatcaac tacgtaataa tctaagttca ttttaaagcc
    16441 cctttctcta atttgttata aatatattct gcacaacaaa gatcttcaat agctagacca
    16501 actgcgtcga aaatcgtcgg cctcgattct cctttataaa atgttttttt ctcagaaact
    16561 aattcgccta atgtaccaac aatacaagat tcctctaaat ctccttctgc catggctata
    16621 agcaggtccc cgctttctgt taaggcagct gagtaatcat ctacatagat ttgagaagtt
    16681 tccatcaacg atgtacttat ctctcttgta tcaggagtaa atgtgcccac agcattgata
    16741 tgtgcgtctt cccttaccca ctctttttga agaaatgcct ctttactaga ggtcaacgta
    16801 caaatgattt ccgctggacg tactgcatct tctactgata ttgcatttat aaacgttatt
    16861 ttaggatact gttttttagc tttagcgata aaagtatttc gtctttcttc taaaatatcg
    16921 taaacaaaca ctgtagtgat gggacgaatg gtagaaatcg cagtcaaatg cgacaatgct
    16981 tgttgaccag ctccaattaa agcaagtgtt tctgcatgcg gatttgctaa gtaatctgtt
    17041 gccagcgcag atacagcagc tgttcgaatc caggtgactg catttgcgtt tatcaatgct
    17101 ttgggtagtc cgttttgagc atcaaacaag aaaatctctc ctaaatgaga aggtaaatga
    17161 tactgatgat tatccggaaa agcggtaatg acttttgtgc caaaatatct gttctccccc
    17221 aaataagcag gcatcatcgc aaacatattt ttgccttttc cgtcaggtaa aatttccacc
    17281 aatcgaggat tttggctgat tttaccactt tcataatcaa aaaaacattt tttcattact
    17341 tgaatcgctt cttcaaaact aaaagaagct actacttcct caaaattaat aattttcata
    17401 gacaattccc tcctcacaaa agattaactt ctaaaatcaa tacaatcaat ggtaaaattg
    17461 tatggtatac aattttaagg agtggagaaa atgcctatta attcttatga caattacccg
    17521 ttaacttggc gaccagacaa acaaaaattg aagcgtcctt attatcgatc gttgattcaa
    17581 caattagaac aagatatttt aaagaataaa ttacagaaaa acactcagtt accctcccaa
    17641 agagagctag ctgattattt agatttaaat tttactaccg ttggcaaggc ttataaatat
    17701 ggaattgaga aaggtttgct gtatacgaat attggaagtg gcacatttat ttctcccaat
    17761 gtctttgagt ccatcaccat ctctaccaat gaagtaaccg aacatatcat tgattttggt
    17821 ctggttagtt cttttgaaga atgtaataaa ttcgttgtcc cgtttattca atcaactagt
    17881 aataacccaa atcttgttgg tttgcttaat taccaagatc ccaaaggcac aaattttcaa
    17941 ttgactactg cagcaaattg gttaaaaact caaggagtca ttccgtcctt agaatctcta
    18001 tctattgttt ccggtgttca aaatggattg gctatcacac tttctgcact tttctctcca
    18061 ggtgatagaa tagcaacaga ccgatataca tacgctaatt tcattgaatt ggctcagtta
    18121 tatcatttag agatcgttcc aattgatttt gacgagcaag gaatgcgacc tgatatattg
    18181 attcaggaat gtagaaaaaa gaagattcat ggtgtctttc ttatgccttc ttgtaataat
    18241 cctatagggt ttcaaatctc tcacaaaaga cgagagcaat tagccgaagt gattgaaaaa
    18301 gaaggtctat gggttattga agatgatatc cattcatttt taacaactta ctatcaacat
    18361 acgatcattc ctcctttcca aaatttatta cctaaccaaa caatctactt agctggaatg
    18421 acaaaattta tttgttctgg attgcgagtt gcttatcttg tagttccagt acatgtccaa
    18481 agcaagatag aacaagcaat ttttaatata aatgtgaaaa cttctggatt agatgtggaa
    18541 gtcatcactc aaatcctcca ttcaaatgaa gcaaatagga tcttagaaac taaattaagt
    18601 cttacaaaaa gagccaatca gctttttgat tctcttttca aagtagaaaa accaacgaat
    18661 cgtgtccctt actatcgaat tcttccagtt gatcctgaga ttgaccctaa gataattgag
    18721 aatgactttc ttgctaacgg tatccgactt tatcattcca gacgctttac tacacgtgcg
    18781 caaccagatg cattcgttcg aatttcccta tcctctaata accttgaaat tttagcttct
    18841 ggactaagta tcgtaaaaga aaaaataaaa aaatatgaat tataacctat tgataaataa
    18901 aggaatcaaa tcttattgat tcctttcaat aaaaaagagg cactacccga ggttcaacaa
    18961 cgggtagtgc ctca
//
LOCUS       DACDUU010000061        18063 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.061, whole genome shotgun sequence.
ACCESSION   DACDUU010000061 DACDUU010000000
VERSION     DACDUU010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.061"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            316..675
                     /locus_tag="IWL30_002085"
                     /pseudo
     CDS             316..675
                     /locus_tag="IWL30_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(970..2103)
                     /locus_tag="IWL30_002086"
     CDS             complement(970..2103)
                     /locus_tag="IWL30_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9706823.1"
                     /translation="MLLNFKMTLTMLAFSLLVTGVILVSFFFLRNNLEGISIASTLGE
                     DVYFMEEGKTVQAETQMEASKSSNNQFMTISMIDKAYQDELFKYLPAMVLVICSAVML
                     LTFILWLVLRKIYSNQILAITRDLTFIDQVPKNTVKDEALNAAFNKLKDKFDGHLEDY
                     RRLNSYLTHEQKNAIAILRTNLEIERQHADQGNLMLLDRMTDSIDDILTLSNNSEDLS
                     TEVVDVSLICAEVCDAYKMNYPNLLFAFTEDQPTLIHGRDRWIYRAVSNLVDNAIKYG
                     EGKLVIVTISNQKGSVIIEVKDQGIGIPQEKQASIFSHQYRVHGLKQDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            complement(2094..2762)
                     /locus_tag="IWL30_002087"
     CDS             complement(2094..2762)
                     /locus_tag="IWL30_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9706824.1"
                     /translation="MRVLVVEDNRELVLSVKKGLEQENISVDVAYDGESGEEKAFINS
                     YDGILLDLNLPKKDGLSVLQSLRAEEVESPVIIITARDEIEERAKGLDFGADDYLVKP
                     FELLELKARIRAVIRRYHGRALPQISIGALKIDPLGRRVSYQKTEVVLKPKEFDILLC
                     IAQRHPAVVSSEEISEHVYDEDFDPFSSVLRVHLARLKKKLAEAAEKEVLKTIRAKGY
                     QLCY"
     gene            complement(3218..4204)
                     /gene="lacD"
                     /locus_tag="IWL30_002088"
     CDS             complement(3218..4204)
                     /gene="lacD"
                     /locus_tag="IWL30_002088"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP9706825.1"
                     /translation="MNEEKRQYIKQLCNEDGVIGALAIDQRGALKKMITKFKGSEAEE
                     SEIVEFKEIVSSQLTKYASSILLDPEYGLPAADVRAKNAGLLLAYEKTGYDASTPGRL
                     PDLLSIWSVKRLKEAGADACKFLLYYDVDEAEEINNQKHAFIERIGSECQAENLPFFL
                     ELVSYDANISDATSKEYAIKKPHKVNDMMKEFSDPRYGVDVLKVEVPVNMNYVEGYGT
                     DEVVYTKAQAANFFKEQAEATNLPFIFLSAGVKAELFQETLRFAKNAGSTFNGVLCGR
                     ATWADGVESYIVDGKEAAMEWMNTQGRKNIEELNAVLKETATPVEVLTSSLS"
     gene            complement(4217..5143)
                     /gene="lacC"
                     /locus_tag="IWL30_002089"
     CDS             complement(4217..5143)
                     /gene="lacC"
                     /locus_tag="IWL30_002089"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAP9706826.1"
                     /translation="MILTITMNPSIDISYPLETFKLDTVNRVRNVRKTAGGKGLNVTR
                     ILKQLDTEVIASGLIGGFLGEEIKKDLTQNEIAHDFLPISGETRNCIAVLHDGKQTEI
                     LENGPVITEQEARAFLQHFEKLAVKAEIISFSGSLPEGLPADFYRQMLQVCQPKPVVL
                     DCSGTALREVLRGSVKPKLIKPNTEELAGLLGREISTELSDLKAALSDSMFDEIEWIV
                     VSMGGDGAFAKHHETFYCVTIPKIDVVNPVGSGDATVAGLTAAIQQGLSDEAVLKQGN
                     VLGMLNAQEATTGYVQLANYQKLYDQILVEKV"
     gene            complement(5156..5671)
                     /gene="lacB"
                     /locus_tag="IWL30_002090"
     CDS             complement(5156..5671)
                     /gene="lacB"
                     /locus_tag="IWL30_002090"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAP9706827.1"
                     /translation="MKIAIGCDHIVTDTKMAVSDFLKSKGHEVLDMGTYDFTRTHYPI
                     YGKKVGEAVTSGEADLGVCLCGTGVGINNAVNKVPGIRSALVRDMTTAIYAKEQLNAN
                     VIGFGARITGEFLICDILEAFINADYQETEENKKLIAKIDHVEKASDKQSDPHFFDEF
                     IEKWDRGEYHD"
     gene            complement(5690..6118)
                     /gene="lacA"
                     /locus_tag="IWL30_002091"
     CDS             complement(5690..6118)
                     /gene="lacA"
                     /locus_tag="IWL30_002091"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HAP9706828.1"
                     /translation="MRVVIGSDSDGMELKELIKKKLIKEGITVVDKSETASEDFVEST
                     LAIANDLNENEGSLGIAVDGYGAGSFITATKVKGMVAAEVSDERSAYMTRKHNNSRMI
                     TLGSKIVGDELALNIVKEFLQANYDGGRHQVRVDMLNKMA"
     gene            complement(6138..6911)
                     /locus_tag="IWL30_002092"
     CDS             complement(6138..6911)
                     /locus_tag="IWL30_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9706829.1"
                     /translation="MLKTERQRSILAMCDRYGTITVKFIQEVLNVSDMTIRRDLEELA
                     QKKKLLRVHGGAQKIDYLEMSTSIEANELSHTEKKKLHTKEKEYIAKNAAAHINQEDD
                     TIFLGSGTTIELMTRYIKPSSLRIVTNSLPVFNLLSEIKRFELYLIGGLYREKTGAFV
                     GSIANEAVQKIGIKKAFVGVNGLKGSEVSNFSIEEGTLQKIVLDNAAEKCLVSDANKF
                     DHSDFYNFYDLRKIDKLFTDSTLSAELKSYYQQFTKIIQ"
     gene            7076..7555
                     /locus_tag="IWL30_002093"
     CDS             7076..7555
                     /locus_tag="IWL30_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9706830.1"
                     /translation="MNEFFFKDTVYVSDKQSQEEVFEEVYQDLLKKELVTEDFLENLL
                     ERERNYPTGLDLSPVSKILPDIAIPHTESEFVRTTRIIPIKVKNEIPFYNMISPSDQI
                     SASFLFMILNENGEAQAGMLANIMDFINSVDEEKLFSFFKLEDTEAIYHFLEEHFYI"
     gene            7612..7914
                     /locus_tag="IWL30_002094"
     CDS             7612..7914
                     /locus_tag="IWL30_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003088862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAP9706831.1"
                     /translation="MIKILAACGAGVNSSHQIKSALETELKNRGYQVTCDAVMIKDIN
                     EEMLSHYDIFAQIANTDLGFDINIPVVDAGAILYRIPAMAEPVYQSMESAIQSLNK"
     gene            7936..9393
                     /locus_tag="IWL30_002095"
     CDS             7936..9393
                     /locus_tag="IWL30_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol transporter subunit IIA"
                     /protein_id="HAP9706832.1"
                     /translation="MNTIIDIANKVFTPLINLGAAPLMTIVLTVIALFFKVKPSRALE
                     GGLKLGIALTGIGAIIGILTTAFSDAMTAFVERTGISLNITDVGWAPLATITWGSPYT
                     LYFLLIMVIVNIVMLAMKKTNTLDVDIFDIWHLAIVGLFAIFCGANLFIASALVIFIG
                     VLKIWNSDLMKPTFNDLLNAPDSNPMTTTHMNYMMNPIIMVFDKIFDKIFPWLDKYDF
                     DAAKLNSKIGFWGSKFAIGIYLGIFVGLLAGQTPTQIFSLAFTAAVCLELFSLIGAWF
                     IAAVEPLSQGITDFANKRLKGRTINIGLDWPFIAGRAEIWAAANVLAPIMLLEAIILP
                     GNKLLPLGGIIAMGVTPALLVVTRGKLIRMIVIGAIELPLFLWSGTLIAPFVTQTAKA
                     VGAFPAGLNGSAMISHTTMEGPIEKFLGYLVGNASQGQIEFVIYAALALGAYLLIFIW
                     YARQMKKRNAAYAAEKEENASPATAHNMTYAAEAK"
     gene            complement(9986..10738)
                     /locus_tag="IWL30_002096"
     CDS             complement(9986..10738)
                     /locus_tag="IWL30_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9706833.1"
                     /translation="MLKTDRFQAIMSLLEVNGTVRVSDIMEKFSVSDMTVRRDLDEME
                     KQGLLERVHGGAKLKDFYRHVELSHREKQIMLQEEKQRIVEKANEMINDGDTIFLGPG
                     TTIELMSKIIQGDSLRIVTNCLPVFEELNKRKGNRKIYLLGGEMRETTQCFVGEITNK
                     SLQDMNFHKAFFSCNALNEQKIMTSTFEEGQTQQIALNNSVERFLLIDHSKIGKRDFS
                     VYYNLNDVTVVITDDDEQKHYEKIEKEEKYVV"
     gene            complement(10953..11189)
                     /locus_tag="IWL30_002097"
                     /pseudo
     CDS             complement(10953..11189)
                     /locus_tag="IWL30_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
     gene            complement(11199..12163)
                     /locus_tag="IWL30_002098"
                     /pseudo
     CDS             complement(11199..12163)
                     /locus_tag="IWL30_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(12369..14051)
                     /locus_tag="IWL30_002099"
     CDS             complement(12369..14051)
                     /locus_tag="IWL30_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9706834.1"
                     /translation="MNTIIKQIEKGKPFFEKISRNIYLGAIRDGFLTAMPAILFSSIF
                     IMIAAVPEVFNVTLPEAFSTWLWKVYGYSMGLVGLLVVATTARCLANSMNRQLSDSGK
                     QINEVSVMLASICGFVMMAADQIDGGIATDYLGTKGLLAAFVSAFVTVNVYKFCVKRD
                     LTIRMPKEVPGTITQMFRDIIPFSIAVFIAVLIDTGVRQFFGTSFAAGVIQLLQPLFS
                     AADGYVGIAIIWGAMAMFWFVGVHGPSIVEPAIAAIIYANVETNFELFKNGAQASKVL
                     TVGLGNFVGTMGGTGATLVVPFLFMLFAKSKQLKAVGKASFIPVSFAVNEPLLFATPI
                     ILNPYFLIPFLLAPIANVWIFKLFVDVLGMNSFISVLPWATPAPIGLVLGTGFSVLAI
                     VLAVVLIVVDAIIYLPFVKAYDSELVEQELKNIAAEEVKEELVEEIQEVGATAAVIVD
                     EAKASVGEGKRVLVLCAGAGTSAMLANALEEGAKETGVNLSAKAGAYGSHYDMMKNFD
                     LIVLAPQVNSYLDDLRKDADKLGIKVIATKGAEYINLTRDPKGAVDFVLKQF"
     gene            complement(14062..14385)
                     /locus_tag="IWL30_002100"
     CDS             complement(14062..14385)
                     /locus_tag="IWL30_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9706835.1"
                     /translation="MDRKELQLLGFEIVAYAGDARSSLLTVLKEVREGNFDNVERLLA
                     DADENLTLAHNSQTKCLAEEASGNEMEVGVIFIHGQDHLMTTILLRELINDFVELYKE
                     KFAKA"
     gene            complement(14408..15814)
                     /gene="lacG"
                     /locus_tag="IWL30_002101"
     CDS             complement(14408..15814)
                     /gene="lacG"
                     /locus_tag="IWL30_002101"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP9706836.1"
                     /translation="MNYKTLPEDFIMGGATAAYQAEGSTKEGGKGQVAWDTFLVEKGR
                     YTADPASDFYNQYPVDAELCKKFKIDSIRMSIAWSRIFPKGYGEVNQTGVEYYHKVFA
                     ELNKHGIIPFVTLHHFDTPKELFDNGDFLNRENVEHFVNYAQFCFDEFSEVHYWSTFN
                     EIYPVASNQYLIGSFPPEVQFDIEKTVTCLHNMMYAHAKTVNIFKDGGYKGEIGVVHS
                     LETKYPASDKEEDKHAAKLDDALSIKFLLDATYLGYYSDETMAHINEILAANGQSADI
                     DSNDFREMKKAANRNDFLGINNYGSHWVKEYKGESTLHYNTTGEKGSQVYCIKGIGER
                     IVREDLPKTDWDWIIYPKGLYDLLVRIQDEYPNYNKIYITENGLGYKDNFEDGIILDD
                     DRIDYLRQHFNAISDAIKMGVNVKGYFIWSLMDVFSWVNGYTKRYGLFYVDFDTQKRY
                     PKESAYWYKFVSETKTII"
     gene            16281..17288
                     /locus_tag="IWL30_002102"
     CDS             16281..17288
                     /locus_tag="IWL30_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9706837.1"
                     /translation="MVTIRDIAKKSGYSTATISRLFKGDESLSITSDTKNKIIVTAKS
                     LGYDLTKIKTTLYKIAILFWISEEQLKKDVYYQHVKDDLKNYSKKANLDLIFISKNDG
                     VKSIPDDITGFIAIGQLTKKELYYLNQKGYKGVIRGINPLPEHFDTVEPDFHEMARQA
                     INYFINEGFTKIGFIGGNFFNLETGKEEIDSREYAFRYHLEKSGLLNEKYIFSQGSFT
                     VDEGYQLTLKMKAELNEDMPEACFIPSDTIAAGVLQGCHELGINIPKDMAVISINDDD
                     IAKFVAPPLTTFKIDIEEIAKTCVDLLSDQLVYSRDISKKTLIGAKLTVRKSFIPKEE
                     V"
     gene            complement(17682..>18063)
                     /locus_tag="IWL30_002103"
     CDS             complement(17682..>18063)
                     /locus_tag="IWL30_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-dehydro-3-deoxyphosphogluconate aldolase"
                     /protein_id="HAP9706838.1"
                     /translation="NSLRYLEFREGAFDFQTSQQNLLTIPGAMSPTEIFKMFEMGADI
                     VKVFPANCLGPEYFNQVQAPLGSLPLMAVGGVDQTNAQNYLNNGASYVGIGSKFFEKS
                     AVHQLNYERLMELAESFIDSLRVE"
BASE COUNT         5431 a         3813 c         2981 g         5838 t
ORIGIN      
        1 tataaataac tcataaaaaa tattttgact ttcaaaatca cttttgctac cagaagttga
       61 ggcgaagtgt tttaagcata gttgtaccct agtttgaccg ttaaaaataa ataacgatcg
      121 ctagagtgca actatgtttg tttgccctac cgtcagcgtc ggcttggcct ctcacatcca
      181 ttaccgtcgg gattatctcc gttacatacc cttacaaaat acgccctgta agcattgcat
      241 gaaaccaccg tgcatttgca ccgtatttcc gccctagccg ttcaaatcgg tcgctaccac
      301 cattcagaaa cacgtgcggt aatggaaggt ttggaggaag ctatccagac cactaacgat
      361 tgtccctatc gtcgtacgtt tcactcagat caaggttggt cgtatcaaat gaaagcttat
      421 agaaacaaat tgaaggagta caaaatcttt caaagcatgt ctcggaaagg gaactgctta
      481 gataattcac ttatggagaa tttttttggc ttattaaaac aagagatttt tcacgggaaa
      541 gtttataact gtttcgagga attaaaatca gccatcgata gctatatcta ttactacaat
      601 aatgaacgga taaaacagaa attaaactgg caaagtcccg tccaatttcg taaaacaacc
      661 gttacggttg tgtaaaaata tcattttaga gcatgaaaaa aagagtggat ttctccactc
      721 caaaaaagtc taacttttgg gggtcactac atttttgatc ctttttattt gattaattat
      781 atccagctgg ataataattt tgggggcaac atttctatct tactattatc ttcctaaagt
      841 agtagacttt cttattggga aaaattacaa caaaaaggag tggcttagtt gacaactcct
      901 ttttgtttac aaatcaattg gatatctata aattgtattt tctaacgaat tttatagaca
      961 tctgatttgt caaattaagc tagacaatat gtctttatct atgcagctca aaaatacttc
     1021 caatggtgtt cgataattta atgattttct tggaatatgg tttcttttcg aggcaacgga
     1081 ttgaataaag gtttcatccg tgtccctaaa gtccattgtt ttaggtaagc catcctgctt
     1141 gagcccatgc actcggtact gatgagaaaa aattgatgcc tgtttctcct gaggaatgcc
     1201 aattccctga tcttttactt caataatcac gctacccttt tggtttgaaa tggtgacaat
     1261 taccagtttt ccttcaccat atttgattgc attgtcaact aaattcgaaa ccgcccgata
     1321 aatccaacga tctcgtccat gaatcagggt tggttgatct tcagtaaacg caaacaaaag
     1381 attcggataa ttcattttgt aggcatcaca aacttccgca cagatcagtg aaacgtctac
     1441 gacttccgtg ctaagatcct ctgagttatt ggataatgtc aaaatatcgt caatactgtc
     1501 ggtcatccga tctaaaagca ttaaatttcc ttgatcggca tgttgccgtt ctatttccag
     1561 attcgtacgc aaaatagcga tggcattttt ctgctcgtgg gtcagataac tatttaatcg
     1621 tcgataatcc tccaaatgac catcgaactt atcctttagc ttattaaaag ccgcatttaa
     1681 tgcctcgtcc ttcacagtat ttttaggaac ttgatcaata aacgttaaat cacgcgtaat
     1741 cgctaagatt tgattactgt aaattttgcg taaaaccaac caaagaataa acgttaataa
     1801 catgacggca gaacaaataa ccagcaccat cgctggtaaa tatttaaata gttcgtcctg
     1861 gtaagcttta tctatcatcg aaatagtcat aaattgattg ttggaagatt tcgaagcttc
     1921 catctgcgtc tctgcttgaa ccgtcttacc ctcttccata aaataaacat cttcacctaa
     1981 ggtagatgcg atactgattc cctccaaatt atttctcaga aagaagaaac tcactaaaat
     2041 cacgcctgtc accaacagtg aaaaagccag catcgttaac gtcattttaa aatttaatag
     2101 catagctggt acccctttgc tcgaatggtt tttaagacct ctttttcagc tgcttcagcc
     2161 agtttctttt ttagacgagc cagatgcact cgcagaaccg acgaaaaagg atcaaaatct
     2221 tcatcgtaga catgttcaga aatttcctca gacgaaacaa ccgctggatg acgctgagcg
     2281 atgcacagta agatatcaaa ttccttgggt ttcaggacca cctctgtctt ttgatagctg
     2341 acccttcgac ctaatggatc aatcttcaaa gcaccaattg aaatctgcgg cagcgcacgt
     2401 ccatgataac gacgaataac cgcgcgtatt cgtgctttta attctagtaa ctcaaagggt
     2461 ttgactaaat aatcatctgc tccaaaatcc aagccttttg ctcgttcttc aatctcatca
     2521 cgtgcagtaa taatgataac tggactttcg acttcttcag cacggagact ttgtaaaaca
     2581 gataatccat cttttttagg cagatttaaa tctagtagta tcccatcgta tgaattgatg
     2641 aaggccttct cttctcctga ctcgccatca taagctacat ctacagaaat attctcctgc
     2701 tctaggccct tttttacgga taaaaccagc tcacgattgt cctcaaccac cagtacacgc
     2761 atgtatattc actcctcata cggatttagt aattacactt atgcttttta gcatagtaca
     2821 cgctcttctt caaagtccac gttctttttg tgaagatttg aagaaattca tacattctgt
     2881 taaaaaacac ataattaaga atgattttac tgtttgaaaa tacctagaat agaaaaagac
     2941 tggaaccttt cctccagtct cactactttc tatttgcttg gttttcttgt atatactttt
     3001 caaagttcgt tggtttatga ccaataagcc tcgttagaat actgaaaatt gccatcaagt
     3061 cattaagtac cacatgtaag aaaatctctc taaagaaaaa ccccttcgtt ttaactcact
     3121 tactatttta gaattcgttc ccctaataat gcagcatgat tttacgcttt atgtataaaa
     3181 aaagactgca agcaattttg cttcacagtc tgatttctta tgatagtgag cttgttaaca
     3241 cttccaccgg tgtagcagtt tctttcaata cggcatttaa ttcttcaata tttttacgcc
     3301 cttgcgtatt catccactcc atcgctgctt cttttccatc aacgatatag ctttctacac
     3361 catctgccca agtcgcacgg ccgcaaagta caccattgaa ggttgagccg gcatttttcg
     3421 cgaagcgcag tgtttcttgg aacaactctg cttttacacc ggcgcttagg aaaataaatg
     3481 gcaggttagt ggcttcagcc tgttccttga agaagttagc cgcttgtgcc tttgtgtaca
     3541 cgacttcgtc tgtcccatag ccttccacat aattcatatt gacgggtact tccaccttta
     3601 acacatctac gccataacgt ggatcagaaa attccttcat catatcgttg actttatgag
     3661 gtttcttaat ggcgtattct ttcgaagtcg catcagagat gttcgcgtca taagaaacaa
     3721 gctccaagaa gaagggtaag ttttcagcct gacattctga gccgattcgt tcaataaagg
     3781 catgtttttg attattgatt tcttctgctt catcgacatc atagtaaagt aggaacttac
     3841 aagcatcggc gcctgcttct tttaaacgtt taactgacca gatcgataat aaatctggta
     3901 aacggccggg agttgacgca tcgtaaccag ttttttcata agctaacagc aagccggcat
     3961 tttttgctct aacgtcagct gctggcaagc catattcggg atctaataaa atcgatgagg
     4021 catatttcgt taattgtgaa gaaacaattt ctttaaattc aacgatttct gattcttctg
     4081 cttcgctgcc cttgaatttt gtgatcatct tcttcaaagc cccgcgttga tcaattgcta
     4141 aggccccaat cactccatct tcattgcata gttgcttgat atattgacgt ttttcttcgt
     4201 tcatttccgt atcctcctac actttctcca ctaagatttg atcatatagt ttttgataat
     4261 tggctaattg gacatagcca gtcgtcgctt cctgagcatt cagcattcct aaaacattac
     4321 cctgttttaa cacggcttca tccgaaagac cttgttgaat cgcagcggtc aatccggcaa
     4381 cggtcgcatc accagagcca actggattga cgacatcaat ctttggaatt gtcacgcaat
     4441 aaaaagtctc gtgatgtttg gcaaaggctc cgtcgccccc cattgaaacc actatccatt
     4501 caatttcatc aaacatcgaa tctgacaagg cggcttttaa atctgataat tctgttgaga
     4561 tttctcgtcc tagcagacca gccagctctt cggtattggg ttttattagc ttaggcttta
     4621 cactgcctcg caacacttcc cttaatgctg tacctgaaca atctaaaacg acaggctttg
     4681 gctgacaaac ttgcagcatt tgtcgataaa aatcagcagg caatccttct ggcaagcttc
     4741 ctgaaaaaga aatgatttcg gctttaacag ctaatttttc gaaatgttgc aagaacgccc
     4801 ttgcttcttg ttcagtaata acaggtccgt tttctaggat ttccgtctgt tttccgtcat
     4861 gtaaaacagc gatgcaatta cgtgtctctc cgctgatagg taaaaagtca tgagcaatct
     4921 cattttgagt caggtccttt ttaatctctt cacctaaaaa accgccgatt aaaccactag
     4981 caatcacctc cgtatctaat tgcttcaaga tacgtgtcac atttaggccc ttaccgccag
     5041 ctgttttgcg aacatttctt actcgattga ctgtgtcgag cttgaaggtt tctaatggat
     5101 aagaaatatc aatcgatgga ttcatcgtta ttgttaaaat catttgtcca cccgcttaat
     5161 cgtggtattc gccacgatcc catttctcga taaattcatc gaagaaatgc ggatcagact
     5221 gcttgtcact ggctttttcg acgtgatcga ttttggcgat caattttttg ttttcttcag
     5281 tttcttgata atcggcattg atgaaggcct ctaatatgtc acagatcaaa aattctcccg
     5341 taatccgagc gccaaagccg atcacattgg catttagttg ttccttcgca tagatagcag
     5401 tcgtcatatc gcgaaccagc gcactgcgga taccaggtac tttattgaca gcgttgttaa
     5461 tcccaacacc tgttccacac aaacaaactc ctagatctgc ttctccgcta gtcacggcct
     5521 ctccgacttt ttttccgtag atcggataat gggttctggt aaagtcatag gtccccatat
     5581 ccaacacttc atgtcctttt gattttaaga aatctgatac tgccattttt gtatctgtga
     5641 cgatgtggtc acagccaatt gcgattttca tttcattcat ccttcctaat tacgccattt
     5701 tatttaacat gtcgacacgt acttgatgac gaccaccatc ataatttgct tgtagaaatt
     5761 ccttaacaat attcaacgcc aactcatcgc caacaatttt tgatccaagt gtgatcatcc
     5821 gagaattatt atgtttacgg gtcatatacg cactgcgctc atccgaaact tctgctgcta
     5881 ccattccttt aacctttgtt gccgtgataa agctgccagc gccgtagcca tcaaccgcga
     5941 ttcctaaact accttcgttt tcattcagat catttgcgat cgccaaagtt gattccacaa
     6001 aatcttctga agccgtttca ctcttatcta ctactgtaat accttctttg atcaattttt
     6061 tcttgattaa ttcctttaat tccatgccgt cactgtctga accaattact acacgcattt
     6121 ttgtttcctc ctatttttta ctgaatgatt ttagtgaatt gctgataata actcttcagc
     6181 tcggctgaaa gagtagaatc cgtaaataat ttgtcgatct tgcgcaaatc atagaaatta
     6241 taaaaatcgc tatggtcgaa tttgtttgca tctgaaacca aacatttttc tgccgcatta
     6301 tctaaaacaa ttttttgcaa ggtaccttct tcaatactga aattcgaaac ctccgaaccc
     6361 ttcaatccat tcactccgac aaaagccttc ttaataccga ttttttgaac agcttcattg
     6421 gcaattgaac cgacaaaggc tccagtcttt tcccgatata gcccaccgat caaatacagt
     6481 tcaaatcttt ttatttcact taatagattg aaaaccggca gactattggt aacgattcgg
     6541 agagatgacg gcttgatgta tcgtgtcatt aattcgatcg ttgtacccga tccaagaaaa
     6601 atcgtgtcgt cttcttgatt gatatgagct gccgcatttt tagcgatgta ttccttctct
     6661 tttgtatgca gcttcttttt ttcagtatga gaaagctcat ttgcttctat agaagtcgac
     6721 atttccagat aatcgatttt ttgtgcaccg ccatgaacgc gtaaaagctt tttcttctgc
     6781 gcgagttcct ctagatctcg gcggatcgtc atatctgaaa cattcagtac ttcctgaata
     6841 aatttgactg ttatcgtccc atagcgatca cacatcgcta agatcgaacg ctgtcgttct
     6901 gttttcagca tattctgtac ctccacgacc ttataataaa acatttacga acatttttca
     6961 acacatattg ttcgtttatt ttcgtttatg cgttgacaat gattttgtaa gcggttatac
     7021 tcaacttgta aacatataaa taaacataaa cgaactaata aaggagctgt tttgtgtgaa
     7081 tgaattcttt ttcaaagaca ctgtctatgt ttctgacaag cagtcgcagg aagaagtgtt
     7141 cgaggaagtc tatcaagatc tgttaaagaa agaattggta accgaagatt ttttggagaa
     7201 ccttttagaa cgggagcgta attatccgac gggattagat ctctcacctg tctctaaaat
     7261 attgccagat atcgctatcc ctcataccga aagtgagttt gtgcgaacca cccgtatcat
     7321 tccaatcaaa gtaaaaaatg aaatcccttt ctacaacatg atctcgccaa gcgatcagat
     7381 ttccgcttca tttcttttca tgatcttgaa cgaaaatggt gaagcacagg ctggcatgtt
     7441 ggctaatatc atggatttca ttaattctgt tgacgaagaa aaactgtttt cgtttttcaa
     7501 attagaggac acagaagcga tttatcattt tttagaagaa catttttata tatagaagtc
     7561 tctgcctaac cgcaggataa aaaataaact tattttaagg ggagaattat tatgatcaaa
     7621 attttagcag catgtggagc aggcgttaat tcaagccatc aaatcaaaag cgctttggaa
     7681 actgaattga aaaatcgtgg ctatcaagtc acctgtgacg ccgttatgat caaagatatc
     7741 aatgaagaaa tgctgtctca ttatgacatt ttcgcacaaa ttgccaacac cgacttgggt
     7801 tttgacatta acatccctgt cgttgatgca ggtgctattc tttatcgtat tcctgctatg
     7861 gctgagcctg tttaccaaag tatggaaagc gcaatccaat cattaaacaa ataggactaa
     7921 agaaaggaaa agcatatgaa tacaattatt gatattgcca ataaagtatt taccccccta
     7981 attaatttag gtgcggcacc gttaatgacc atcgtattaa ctgttattgc tttatttttt
     8041 aaagtaaaac cgtcacgcgc tcttgaaggc gggctcaaat tagggatcgc cctgactggg
     8101 atcggagcta ttattggaat tttaactact gctttttcag acgctatgac agcttttgtt
     8161 gaacgcacag gtatttcatt aaacattaca gatgtcggtt gggccccact agcaaccatc
     8221 acatgggggt cgccatacac gctttatttt ttactaatta tggtgatcgt caatattgtc
     8281 atgttagcaa tgaaaaaaac caacacatta gatgtggata ttttcgatat ttggcattta
     8341 gcaattgttg gtttgttcgc gatcttctgc ggtgctaatc tgtttattgc ttctgcatta
     8401 gttattttca ttggtgtatt aaaaatttgg aactccgact taatgaaacc aaccttcaat
     8461 gacttgttaa atgcgccaga cagcaacccg atgaccacga ctcatatgaa ctacatgatg
     8521 aatccgatta ttatggtttt cgataaaatt ttcgacaaaa tcttcccttg gttggataaa
     8581 tatgatttcg atgctgccaa attgaatagt aaaattggct tctggggatc aaaattcgcg
     8641 attggaattt atttggggat ttttgttgga ttactagcag gacaaacacc aacgcaaatt
     8701 ttctcgctag cctttaccgc tgccgtctgt ttggaattgt tctctttgat aggcgcttgg
     8761 ttcatcgcag ccgtagaacc gttatcacaa gggattaccg attttgccaa taaacgacta
     8821 aaaggacgta ccatcaacat aggtttagac tggcctttca tagcaggccg cgctgaaatc
     8881 tgggctgccg ctaacgtatt agcaccaatc atgttgctag aagccatcat cttaccagga
     8941 aataaattat tgccactagg cggaatcatc gcaatgggtg ttaccccagc actattggtc
     9001 gtaactcgtg gaaaattaat ccgtatgatc gtaatcggcg cgatcgaatt accattattc
     9061 ttatggtccg gcaccttgat cgccccgttc gttactcaaa cagctaaagc agtcggagcc
     9121 tttccagccg gcttgaacgg gtccgccatg atctctcata caacgatgga aggcccgatt
     9181 gaaaaattcc taggttattt agtcggtaat gcttctcaag gacaaatcga atttgtgata
     9241 tacgctgcct tagcattagg tgcatacctt ttaatcttca tttggtacgc tcgtcaaatg
     9301 aaaaaacgca acgctgcata cgcagctgaa aaagaagaga atgcatctcc ggcaacggcg
     9361 cataatatga cttatgcagc cgaagctaaa taagctaaaa acaccccgaa tgtctttaga
     9421 cttcggggta ttattttacc tataaaaaat aaatagatgt aaaatccatt tcaaaaagca
     9481 tagcccatct cttgccacac ccaataaaga aattatcaac gtttatgctt tttttaactc
     9541 ctttaagctt gcctttgttg gtattcctca acgattcttt ttgtgttctt gtttgttttg
     9601 tgctttttga ctttttgttg tttaattgcg aattccttga tttctttttt cctgaatcgc
     9661 ttcgcatccc gcggttatgc gtgcgatttc atcattgctt tttctgatct aactcgtttc
     9721 atgtcaaaga gcttcagctg acttagtgtc catggttgtt ttgatatccc ttttcctcct
     9781 taataaagcg gtgattgata ctgctatttt ttccttcaca taaacataac atcccaaaaa
     9841 aatcgactat cttttttcaa atggtgtatt caagtattcg gaggaattag taatgatgca
     9901 ccctaaaaat aacataattc gcttaaattc aaggctacca aaaaactacc aagttctaat
     9961 tatctagagt ttggcagttt ctcatttaca caacatattt ttcttctttt tcgatttttt
    10021 catagtgctt ttgttcatca tcatccgtta taactactgt tacatcattc agattataat
    10081 atactgaaaa atctcttttc ccaattttcg aatgatcaat caataaaaag cgttcaacag
    10141 aattattcag agcgatctgt tgagtttggc cctcttcaaa agtcgaagtc ataattttct
    10201 gttcatttaa agcattacag ctgaaaaatg ctttatgaaa attcatatcc tgtagcgatt
    10261 tattcgtgat ttcaccaaca aagcattgag tcgtttcccg catctcgcca cctaatagat
    10321 agatttttcg attccctttt cgcttattta attcttcaaa aacaggcaaa cagtttgtaa
    10381 caatccgcag tgaatcccct tgaatgattt ttgacatgag ctcgattgta gttcctggtc
    10441 caagaaatat cgtatcacca tcattgatca tttcattcgc cttttccaca atccgctgct
    10501 tttcttcttg cagcataatc tgtttttctc tatgagaaag ctccacatgc cgataaaaat
    10561 ccttcaattt cgctccgcca tggacgcgtt ctaataatcc ttgtttctcc atttcgtcca
    10621 aatcacgtcg gactgtcata tccgaaaccg aaaacttctc cataatatcg ctgactcgta
    10681 ctgtaccgtt aacctctaat aacgacatga tcgcttggaa tcgatccgtt tttaacattt
    10741 catcgcctct tttcttttta agactatcaa attcttaaga aaagtgctag aacaagtttt
    10801 cttaagaagc cttctgcagg aaggaaagca aaatttttca gaatttgcaa gctgctattc
    10861 taaaaaataa aaccgaacga gcatgactgt catagtgaca gccttgttca ttcgactttt
    10921 atctttaata aatcatttta tttaatctga agttattgtg caagttcaaa aacaaagtag
    10981 ttaatgtctc ctcgatattt cgaaatcgaa tactcaacgg gcagacgttc tagcgaaaaa
    11041 ccctgtgtat ggaaatagaa cattggatct ccttcttcca catctaacaa atcgctgatc
    11101 gtatcatccg ctttgattac ttctagtttt ctggtgaccg ttttaattgg ataacccatt
    11161 tcttcaaaaa cttgatacat tttttctttt ctgatttgtc aaattaagct agacaatatg
    11221 tctttatcta tgcagctcaa aaatacttcc aatggtgttc gataatttaa tgattttctt
    11281 ggaatatggt ttcttttcga ggcaacggat tgaataaagg tttcatccat gtccctaaag
    11341 tccattgttt taggtaagcc atctcgtcta agcaagccgt ttgagtgctc gtgctcattt
    11401 agccctcttt gcgaaggtgt tcctggatct gcgaaaaaga tggcaatatc gttcgagtta
    11461 cttatagatc gccagttgga aaattctttt ccacaatcaa atgtcatgga tttaaacaag
    11521 tgtttaggca tggatttgaa ccaatcattt aacctgtttt cgatatcttc ggctcgtctg
    11581 cctgtcggtt tcaaagtaat aataaccttg gataaacact caaccaatgt aataacagcg
    11641 ctcttgtgtt taactcccac aatggtgtcg ccttcgagat gaccaaattc tttagtcagg
    11701 tgtggataga gactgttccg ttcatgaatc gttctgcgaa aggcctgttt tccacgcttt
    11761 tcttgatgat tattcggttt acgtttcccc ttcataggta acgactgtgt gtcaaaaagg
    11821 ccttttttaa acatccgata gagtgtacga acagaacaat caattggaaa ctcgccacgg
    11881 ccgataatca catcgggtga ccacccttga gctacctttt cttgaacata tgttgattgt
    11941 tcatcagata aaacggttga acgtcgtcca caacgagatt tattttgttt gtattgacgg
    12001 taataatcta gcactttttt ccttgtttga gaaacgaata aacattataa atggtttgtc
    12061 tagagcgatt taaactttgt gcgacaagag cgacaggttt ctcgatattg aaataagact
    12121 ctatgattac tagttcgtct gttgtaagat gggtgtaggt catttgtact cactccttat
    12181 taattctttg gtcggaacta ttttgagtgt agcacaaatg atctttttag ttgtctagct
    12241 taattttaca atcgacgcct caaaaagaat acccgaaatt taaacagcaa aaaaagatag
    12301 cgcttaccat tgtgtttaag ataaaacgct agctaagttg gctagcgctt tattaatcta
    12361 agcaagctct aaaattgctt caatacaaaa tcaactgctc ctttaggatc acgtgttaaa
    12421 ttgatatatt ctgcaccttt agttgcaatt acttttatcc ctaatttgtc tgcatcttta
    12481 cggagatcat ccaaatagga attcacttga ggtgctagaa cgattaaatc aaaatttttc
    12541 atcatatcat aatgagaacc ataagctcct gctttagcag aaagattaac acctgtctct
    12601 tttgcgccct cttctaatgc attagccaac attgcactcg ttccggcacc agcacaaagt
    12661 actaaaacgc gctttccttc accaaccgat gcttttgctt catcaactat aactgccgca
    12721 gttgcaccaa cttcttggat ttcttcgacc aattcttctt ttacttcttc tgcagcaatg
    12781 ttttttaact cttgttctac taattcagaa tcataggcct taacaaaagg taaataaata
    12841 atcgcatcga caacaatcaa tactaccgct aatacaatcg ccaacacact aaacccggtt
    12901 cctaaaacca agccaatcgg tgcgggcgtc gcccaaggca atacggatat aaagctattc
    12961 atccccaaga catcgacaaa aagtttgaat atccatacgt tagcgattgg tgctaataaa
    13021 aatggaatta gaaagtacgg attcaaaata ataggagtag caaatagtaa cggttcattt
    13081 accgcaaacg aaactggaat aaatgaagct ttaccaactg ctttcaattg ttttgattta
    13141 gcgaacaaca tgaacagaaa aggaacaact agtgtcgctc cagtaccacc catcgttcca
    13201 acaaagtttc ctagaccaac tgttaatact tttgaggctt gtgcaccgtt tttaaataat
    13261 tcaaagttag tttctacgtt cgcataaata attgcagcaa tcgctggttc aacaatcgat
    13321 ggtccatgaa caccaacaaa ccagaacatt gccatggctc cccaaatgat tgcaataccg
    13381 acatatccat cagcagcact aaataatggc tgcaataatt gaattacacc tgctgcaaat
    13441 gatgttccaa aaaattgacg aactcccgta tcaatcaaaa cggcaataaa tactgcaatt
    13501 gaaaagggga taatatctct aaacatctgt gtgattgttc cgggaacttc ttttggcata
    13561 cggatcgtca aatctctttt aacacagaat ttatacacat ttacagtaac aaacgcagaa
    13621 acgaaagctg ctaataatcc ctttgtccct aaataatcag tagctatccc accatcaatt
    13681 tgatcagctg ccatcattac aaaaccacaa attgacgcta acataacaga aacttcatta
    13741 atctgcttac ctgaatcaga taattgacgg ttcattgaat tagctaaaca tcgagctgtt
    13801 gtagcaacaa ccaaaagacc aactaatccc attgaataac cataaacttt ccaaagccac
    13861 gtcgagaatg cctctggtaa cgtcacgtta aatacttctg gaacagccgc aatcataata
    13921 aaaatacttg aaaatagaat agctggcata gcagttaaaa agccatcgcg aattgcccct
    13981 aaataaatat tacgtgagat tttttcaaaa aacggttttc ccttctcaat ttgcttaata
    14041 atcgtattca tttttcttct cctatgcttt agcaaatttt tctttgtaca attcaacaaa
    14101 atcattgata agttctctta atagaatcgt tgtcattaaa tggtcttgcc catgaataaa
    14161 aattacgcct acttccattt cgttccctga cgcttcttca gctaaacatt tcgtttgtga
    14221 gttatgcgct aaggttaaat tttcatcagc atccgctaat aatcgttcaa cattatcaaa
    14281 attaccttct ctcacttcct tcaacactgt taataatgaa cttctggcat ctcctgcata
    14341 agcaacaatt tcaaatccta ataattgcaa ttctttccta tccatttcct tgccctccta
    14401 actattttta tattatcgtt tttgtttcgc taacaaattt ataccaatat gcagactctt
    14461 tcggatatct tttttgtgta tcgaaatcta catagaatag tccataacgc ttagtgtatc
    14521 cattaaccca agagaaaaca tccattaatg accaaatgaa ataccccttt acattaactc
    14581 ccattttgat agcatcggaa atcgcattaa aatgctggcg taaataatcg attcgatcat
    14641 catctagaat aatcccatct tcaaaattat ccttataccc tagcccgttt tcagtaatgt
    14701 atattttatt ataatttgga tattcgtctt ggattcgtac aagtaaatca tataaccctt
    14761 ttggataaat gatccaatcc caatcggttt tcggaagatc ctcacgcacg attctctcgc
    14821 caatcccttt aatgcaatag acttggctac ctttttctcc ggtagtgttg tagtgaagtg
    14881 tgctttctcc tttgtactct ttaacccaat ggctaccata gttattgata ccaagaaaat
    14941 catttcgatt tgctgctttt ttcatctctc taaaatcatt agaatcaata tctgcagatt
    15001 gcccatttgc tgctaaaatc tcattaatat gtgccattgt ttcatctgag taatacccta
    15061 aataagtagc atctaataag aacttaattg ataacgcatc atctaatttc gcagcatgct
    15121 tatcttcttc tttgtcactt gctggatatt ttgtctctag cgagtgaaca acaccaattt
    15181 cccctttata tccaccatct ttaaaaatgt ttactgtctt tgcgtgcgca tacatcatgt
    15241 tgtgtaaaca agttacagtc ttctctatat caaattgaac ttctggagga aacgatccga
    15301 tcaaatattg atttgaagca actggataaa tctcattaaa tgtactccaa taatgaacct
    15361 cagaaaattc atcgaaacag aactgtgcat agtttacgaa atgttcaaca ttttctcgat
    15421 ttaaaaaatc tccattatca aatagttctt taggggtatc aaaatgatga agagttacaa
    15481 aaggaataat accatgctta ttcaattcag caaatacctt atgataatat tccactcctg
    15541 tttggttcac ttccccatag ccttttggaa aaatacggct ccatgcaata gacatcctaa
    15601 tactatcaat tttaaatttt ttgcataatt cagcatcaac tggatattga ttataaaaat
    15661 cactagctgg atcagcagta tagcgtccct tttctacaag gaaagtatcc cacgctactt
    15721 gcccttttcc accttctttt gtagagcctt ctgcttgata agctgccgta gctcctccca
    15781 taataaagtc ttctggtaaa gttttataat tcattttaat ctctccttga aaattagttt
    15841 gatcaatatg ccaataatcc ataaatcacc tcagcataat tttaacatgt ataagtaaat
    15901 ttttttacac aacgaaaaac cgacctcgaa tatctatttt attgcgatat acaagcactt
    15961 atatcccttg atgtaaaata aatataccat tcaaaaaccg ttttgtaaac gtttttttgt
    16021 aaaataaatt tatcttttta ctaatttaat ttacacgaaa caagaaaacg gactgattcc
    16081 cactaaaatc aatcccacct actaatcatt ctccaagtgg ctatccatta tcgattcaat
    16141 cttctctgtt aatggattag gctctctgct gatatcagag tttcgtttcc tgctagaaaa
    16201 agtaatttaa gttactaaaa gagcagtttc gatttacttt aacaaagaaa aggattatta
    16261 tatccctagg aggatgaaca atggttacta ttagagatat tgcaaaaaaa tctggttact
    16321 caacggctac tatatcaaga ctttttaaag gtgatgagtc actctctatt acatctgata
    16381 ctaaaaacaa aataatcgtt acagccaaat ctttaggata tgatctgaca aaaattaaga
    16441 ctactctgta caagattgcc attctttttt ggatctcaga ggaacagtta aaaaaggatg
    16501 tctactatca acatgtcaaa gatgatttga aaaattatag taaaaaagct aatctagatt
    16561 tgattttcat ctctaaaaac gatggtgtaa aaagcattcc tgacgatatt actggattta
    16621 tcgcaatagg acaattgaca aaaaaggaat tgtactactt aaatcaaaaa gggtataagg
    16681 gggtaatccg aggaataaac ccattacctg aacacttcga tactgtagaa cctgatttcc
    16741 acgaaatggc cagacaagca attaactact ttattaatga aggtttcaca aaaattggat
    16801 ttattggagg aaatttcttt aacctagaaa cgggtaaaga agagattgat agccgtgaat
    16861 atgccttccg atatcattta gaaaagagcg gactactcaa tgaaaagtat attttctccc
    16921 aaggatcatt tactgttgac gaagggtatc aattaactct taaaatgaaa gctgagttaa
    16981 atgaagatat gcccgaggca tgtttcatac catctgacac tatagctgcc ggcgttcttc
    17041 agggctgcca tgaattgggt attaacatcc ctaaagatat ggcagtaata agtattaatg
    17101 atgacgacat cgcaaaattc gttgcaccac cacttacaac ttttaagata gatatcgaag
    17161 agatagctaa aacctgtgta gatttattaa gtgatcaact ggtatattcg cgggacatat
    17221 caaagaaaac attaatcggc gcaaaattga ccgttaggaa aagttttatc cctaaagaag
    17281 aggtgtaaaa taagtaattg aaacttatct ttaaaactat aattgtgtcc acatagtagc
    17341 cgagcaatcg ggtttatgcc tattatgttt tccataaata atttgatggt gatattttga
    17401 cgtaaataca atatgatttt ttcatcgcca aattgtgcgc gctaattttt gtttatcatt
    17461 cagaacattt cctccttttt atctaagtat gaggttggat aaacgcttca tcttagtatc
    17521 ttgggggatt ttttatctcg ttgggaatat atcaagcatc agagcgcgca aaaagaactg
    17581 gtcgaaaaat caatctacca gttcaatctc attcactgtg atactatgtt ttatacagat
    17641 aacgccatat tataaaacaa gttgtattcc gcaaatgggt tctattcaac tcttaaacta
    17701 tctataaaag attcagccaa ctccattagc ctctcgtaat ttagttggtg aacagcactt
    17761 ttttcaaaaa attttgaacc aattcctaca taagaggctc cattgttcaa atagttttgt
    17821 gcattagtct gatctacccc tcctacagcc atcaaaggta aacttcctaa aggggcttgc
    17881 acttgattga aatattccgg tccaagacag ttggcgggga acaccttaac aatatcagca
    17941 cccatctcaa acattttgaa tatctctgtg ggcgacatgg ccccaggaat tgttagaagg
    18001 ttctgttgcg aagtttgaaa atcaaacgcg ccctctctga actccaaata tcttaggctg
    18061 tta
//
LOCUS       DACDUU010000062        17979 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.062, whole genome shotgun sequence.
ACCESSION   DACDUU010000062 DACDUU010000000
VERSION     DACDUU010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.062"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(26..724)
                     /locus_tag="IWL30_002104"
     CDS             complement(26..724)
                     /locus_tag="IWL30_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAP9706839.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(717..2069)
                     /locus_tag="IWL30_002105"
     CDS             complement(717..2069)
                     /locus_tag="IWL30_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAP9706840.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2292..3692)
                     /locus_tag="IWL30_002106"
     CDS             complement(2292..3692)
                     /locus_tag="IWL30_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAP9706841.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3853..4590
                     /locus_tag="IWL30_002107"
     CDS             3853..4590
                     /locus_tag="IWL30_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAP9706842.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4715..5194
                     /locus_tag="IWL30_002108"
     CDS             4715..5194
                     /locus_tag="IWL30_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAP9706843.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5191..5958
                     /locus_tag="IWL30_002109"
     CDS             5191..5958
                     /locus_tag="IWL30_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706844.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6188..6757
                     /gene="lepB"
                     /locus_tag="IWL30_002110"
     CDS             6188..6757
                     /gene="lepB"
                     /locus_tag="IWL30_002110"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP9706845.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6861..7463)
                     /locus_tag="IWL30_002111"
     CDS             complement(6861..7463)
                     /locus_tag="IWL30_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9706846.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7569..8038
                     /locus_tag="IWL30_002112"
                     /pseudo
     CDS             7569..8038
                     /locus_tag="IWL30_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8144..8728
                     /locus_tag="IWL30_002113"
     CDS             8144..8728
                     /locus_tag="IWL30_002113"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP9706847.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8731..9804
                     /gene="prfA"
                     /locus_tag="IWL30_002114"
     CDS             8731..9804
                     /gene="prfA"
                     /locus_tag="IWL30_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAP9706848.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9797..10636
                     /gene="prmC"
                     /locus_tag="IWL30_002115"
     CDS             9797..10636
                     /gene="prmC"
                     /locus_tag="IWL30_002115"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP9706849.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10629..10808
                     /locus_tag="IWL30_002116"
     CDS             10629..10808
                     /locus_tag="IWL30_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706850.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10830..11855
                     /locus_tag="IWL30_002117"
     CDS             10830..11855
                     /locus_tag="IWL30_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAP9706851.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12030..13274
                     /locus_tag="IWL30_002118"
     CDS             12030..13274
                     /locus_tag="IWL30_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAP9706852.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13427..14374
                     /locus_tag="IWL30_002119"
     CDS             13427..14374
                     /locus_tag="IWL30_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9706853.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14508..15137
                     /gene="upp"
                     /locus_tag="IWL30_002120"
     CDS             14508..15137
                     /gene="upp"
                     /locus_tag="IWL30_002120"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAP9706854.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15276..15518)
                     /locus_tag="IWL30_002121"
     CDS             complement(15276..15518)
                     /locus_tag="IWL30_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706855.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15639..15711
                     /locus_tag="IWL30_002122"
     tRNA            15639..15711
                     /locus_tag="IWL30_002122"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15672..15674,aa:Thr,seq:cgt)
     gene            complement(15830..16468)
                     /locus_tag="IWL30_002123"
     CDS             complement(15830..16468)
                     /locus_tag="IWL30_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP9706856.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16591..17223)
                     /locus_tag="IWL30_002124"
     CDS             complement(16591..17223)
                     /locus_tag="IWL30_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP9706857.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17574..17873
                     /locus_tag="IWL30_002125"
     CDS             17574..17873
                     /locus_tag="IWL30_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP9706858.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT         6158 a         3150 c         3555 g         5116 t
ORIGIN      
        1 tgtcacattc ccctttttcg aataactagg ctgtttgtcc ttcaagatag ggcagatcca
       61 attctttacc gtaacgttgt tctaaagcta ggcggatcaa acgagctgcc aggttctcat
      121 ttcttgcaag gataggtcca tggaaataag aaccgaagac atttcggtaa atgacacctt
      181 cagattggtc ttcaccatta tttccttgac cttgtaccac tttccccaaa ggtcgttcac
      241 cttcacctaa gaaagtccga ccattgtggt tttcaaatcc ataataagtt tcattgaatt
      301 cttcgttatg gataacgata tcaccgataa accggttatt ttcctgactt aacgtgtagt
      361 gatccagtgc actgattcct tggatctttt ctccattagc tcccatgtag tagtgaccta
      421 gcaattggta gccaccacaa atggctaaca tgacgccatc attttcgata tagttgatca
      481 aagaatcttt ctttttctgg atgtcttttg aaatgattaa ttgctcaaag tcttgaccac
      541 cgccaaagaa aaccaagtca tatttatcag catcaaaagg ttcatgtata ctaacgatct
      601 ccgtatggca agaaaccccc attttttctg ctatatattt caacatcaaa aggtttccgt
      661 tatcaccata tgtgttcatc aaatttccat aaaggtgggc gatattgagt tcataattag
      721 ccacggttca ttcctccttt gataaatcct tgagcagcta gttctttacg aagctgcaat
      781 acagcagtgt aagtagctaa aatatagaca tgctcagttg gcaattcttt gattttttcg
      841 attacttctg gtaattcttt tgtttctgtc aatttatttt ctggaatccc ggcaactttt
      901 agacgtaaag tcatatctgt atgacggtct cctccagaaa taacagctgg gatatccata
      961 tcggcaaagt tttcaaaatt accatcccaa atccagctga catcgattcc gtctgcgtaa
     1021 ttggcattta ataaagccac taaagaaaat ggataaggag ctagaccaat catatcgatt
     1081 acttgattga tacctacagg atttttgaca agtactaatg tacatttttt cccattgata
     1141 ttgatgactt cttgtcggcc gaatactttc tcgtcatatc ctaaaccttg tttgatttta
     1201 tcaggatcga cattgaagta ctctgccact gcagtagctg ctaaagcatt gtagacatta
     1261 tacataccgc caacttcgat tccgtattca tgaccatcga tcacgaaatc tgctgacaca
     1321 ttgtccatcc gtaccatatc agttaatcga taatccaatt caggacgttt gaagtcacaa
     1381 tttggacagt aatatttccc taagtttgca taagtgatca ttttataatg caagatgtga
     1441 tgacaatttg gacataagac accgtctgta ttgtaatgag ccatttgttc acgatcttct
     1501 tcatggtcaa agccataata ttttctagga ttgactgttt cgatagaatt gaagattggt
     1561 gaatcaccgt tactgataat cgttgcattt ggtgactttg cggcaccgtc tacaatcatt
     1621 ttataggtcg tatagatttc tccataccga tccatttgat cacggaaaat atttgtaaat
     1681 aagaacagtt ctggtttcat atattctgtg actttgctca aacttgcttc atctatttct
     1741 aggacagcaa actttttctg tccttttttt ggttttgcct gcaagaaagt cgatacaatt
     1801 ccctgtacca tattcgcacc agtagggttg gttagaactt catcgaattg ctggcgcaaa
     1861 atattgactg ttaaagcggt agtcaatgtt ttaccgtttg ttccggtgac aacgacgact
     1921 tggtagtctt ttgccaaact atctagtata tgtggatcga ctttcagcgc tagtttacct
     1981 ggtaaacttg atccgccttt aaaaaaagtt tttaatgccc attgcgacgt ttttccaact
     2041 gcaatcgcaa aatgactgcg tattcccatt atgtaacctc caaaaacgat ttgctgacga
     2101 ataaattcaa ctaatcatac catatgttga aaaaaaggtt aaattgcttt tcttattctt
     2161 aagaaaaggt tacggctgaa agtgcatgaa atcggacaaa atcgcatgat ttaacaaaaa
     2221 aacagcttga aacaaaatga ttgtttcaag ctgctgtgat tttattattc cctcttacct
     2281 atattcccat tttaaccgat aataattttc tcgcttggat attcatagcc tagatctcgt
     2341 gtttcttttg gcacgaataa catcaatgta tatagcattc cgatccgccc aataaacatc
     2401 agtgtagcaa tcgtgatttt gcccacaaca gacaagtctc cggtaatccc taaagataaa
     2461 ccagttgttc caaacgcaga agttacttcg atgatgatcg agatcagcgt ttgttcttct
     2521 gtagcagata aaaaaacgat acaaaagaaa cacataccaa gagaaagcat aaagacaacg
     2581 actgattttc gcacatcatc ttgatcgatt ctccgtccaa aaatattgat gttatcttca
     2641 cttttgagga aggaatatag atacaaaccg atgattgcta cagtagttgt tcgaatcccg
     2701 ccgcctacag aactaggact gcatccgata aacatcagta aagaaaaaat gatcagtgtt
     2761 gtgatttgaa aatcacccaa atcgtgaatc tgcagaccag cattacgagt cgtaatcgaa
     2821 taaaacatcg aattgatcca tttgacactc atattggaat cttggaataa atggtccttt
     2881 tccaatagat aaatcaagac agttcccgaa acaaacaaga taacaaaagc taacacagct
     2941 aatttggtaa acaaggaaaa acgaaacgga agcttcgcat tggaacgttt gtataatagc
     3001 cattcccggc actccatcaa tacaggaaaa ccaattcctc cgacaaaaat caaaaacatg
     3061 atcaggatca agaaaaaata gtcatgtgca aagggcttga tggaatcccc agtgacgtca
     3121 aacccagagt tcgtgacagc cgatatcgcc tgataaaaac cgtaaaaaac ggcatcacgc
     3181 caacgatcaa aatagcctct gtaataaaaa taaatagaaa agaatgtccc aaaaagtaat
     3241 tgaaaccaga ttaaaatagc aaaagtgatc cgaatcagtc ggacgatacc agacaaacgc
     3301 ggttgattca tatccgtcat gatcagctgt cgctgtttca gtgtgatccg tcgtttagaa
     3361 aaaatgacaa aagcagtaga aatcatcatg atccccaaac caccaacttg aaataatatt
     3421 tccagcagta taataccgtt atcattgaac acagaattga tatcaaaagt cgatagtcca
     3481 gtcacactca ctgtactgat tgccatgaac agcatgtcca caaaagaaac atggctatct
     3541 ggttcgcgaa aaatcggcat ataaaataga acgagtgata agaccgtcat caatatataa
     3601 taaaaaacga tgatttggat cgatgagaaa tgattggcag caaacttctg tcctcgccgc
     3661 tgcagccttc gaaagaataa atcaacatgc acagtactat cctcctgaca atagtcgttt
     3721 ctaccagtat acaaggggaa ttaggaatac tcaaagaaat tatcaaacag ataacaatac
     3781 agctttcgat taaaatagaa atggcttata ataaggaaaa tcctagaaaa aagaaggagg
     3841 aggaataaca aattgattaa tgctcaacaa atcgtagaaa aaatgaaaaa tcaaaaaata
     3901 aattatgata cagttctgaa aaaaatgatc atgcaatggg agaaagaaga agtaaagcct
     3961 aagatattaa tacatacatg ctgtgctcct tgcagtacgc acacattgga attcatgtct
     4021 caatatgcag agatcacgtt atttttttca aattcgaata ttcatcctaa aagtgagtat
     4081 ttgcgaagat tacatgaaca aaaacgattt gtagaccaat ttaatgagaa aactgaccat
     4141 catgttgcgc taatcgttga tgaataccgt ccaaatgaat tcaccaaaat ggttttaaca
     4201 aaccatcttg aaaacgaaaa agaaggtggt gctcgctgca gtgcatgctt caatatgcga
     4261 ttagatcttg ttgcgcaaaa agcgcaagaa ctaggatacg attactttgg aagtgccctg
     4321 accatctcgc caaagaaaaa tgcagctttg atcaatcaga tcggaatgga tatccaaaag
     4381 atttacggga cacaatactt gccaagtgat ttcaagaaaa acaaaggcta tgaacgctct
     4441 ttgcaaatgt gcaaagagta tgatatttat cgccaatgtt attgtggttg cgtgttcgct
     4501 gccaagcagc aaggatgcga tctgaaagaa atcaatcggg aagcaaaagc ttacttaaaa
     4561 acatcaaccg tagtttttga ggaaaattaa agaaatcttt cggaaaaacg taagaaaaaa
     4621 ataaagaatt tatggtagaa ataaggtaga ctgtataatt tgtccgtagt aaagtaagga
     4681 aacaagaata aatttcaatg aaggaagaga taagatgcga aaagtagcac ctattttgaa
     4741 gccaatcgct gtattaacaa tctcgacgat tgtcgcgact actacaggta ttgtgaatag
     4801 ttccagctac cgtgcaccaa gttcaagttc tagtactgta gaagaaacaa caccaactac
     4861 ttcagtctcg acatctgaac caacaacaac ggaaagtttg aatgaaacca ctacgacaag
     4921 tacatgggac gatacaacaa ctagttcgtc gtatgatgag acatatacaa ctgacagctc
     4981 aagcgaaaca agtaatacgg taccagatgg cactccatcg gattcaacca atccaagtac
     5041 aagtccggat acaagtgcta gctccgaaac gataccaagc agtacgactg tagatgataa
     5101 cacgcaaaat gatgatgcag cacgtacagc acagcaaaac caagattcag gaaataccca
     5161 aacgtataac cagcaagcag gtgattctca atgacatttt tactagacct tatcaacggg
     5221 gtgcataaat tccttggcta tttagacatc agtcctaaat atcttaaccg tggatatacg
     5281 attttaagtg tgattcccac gctttattta ttgagaattg tttatggact ttggcaaaac
     5341 caaaactatc ttcaattttt cttatatgga cttgcatttc tagtgcttgt ttattttact
     5401 gttttaaatg tattttatta ctttttagat aaaaacacga aagccgatgt cactcagctt
     5461 tttgtgaagt atttacctga tgaagcgttc aacatccaga cagaagaatc gatagataat
     5521 ggaaattcga tcgataaagt gaatacacaa gaggtcatgg tttcttatga cgaagattat
     5581 caattgaaat tagccgagaa tatgcggtat ttgattggaa atggtgagat caaaacaaat
     5641 gaactcggtc gcatggatgg atttctggtc gatagaaata cattgtatcc ttattactat
     5701 gtcaagaaaa caggagagaa agagtatgtt ctaaatattg gacgaagcta ctcggacatg
     5761 gaaaaagtag ggaccattcg acttgactca acagaagaca cattggaacc tgtcggattg
     5821 ttcatcgtcg gcggtgattt tgtcaaagac gggttacgct atcatgagcc ttatcgtctg
     5881 aaattgattg caaaaaaggc acagtcgcag gaagaaccag tggtttatag tcgtagccaa
     5941 agaagaagac attcataatg tgatgaaacg ccgtgtatca gtggttgcga taaaaacgtt
     6001 tattcggaat aagggggatg tgacacgtct tttgtcacgt tcctttttta tataacgaga
     6061 aagataagac gaacacatca cttattcttt cctttcattt ataaacattg tagaataaag
     6121 aaagtacaac gaagtattgt gaaaaaacgt aataaataac gaggaaaata caagtaggag
     6181 gtaagctgtg aaagaatttt tgaaaaattg ggggattttg atccttgtat tagcggctat
     6241 tttattagct agagtgtacg tgtttacacc agttactgta aacgggcatt cgatggaccc
     6301 tacattaagt gatggacaac gattgatttc atctaagatc tcaaactatg aacgaatgga
     6361 tattattact acaaaggaac cgggggatga agaacggatg attgttaaac ggatcatcgg
     6421 tatgcctgga gatacagtca agatggaaaa tgaccagttg accattaatg gcaaaaagta
     6481 tgatgaacct tatctggatg agttcaaaaa agaattttca gaagataagt tacaaggaga
     6541 atatgcttat agctcaggat tccaagcaca agcagaaagt tcatccacct ttacgagtga
     6601 ctttgaatat actgtgccaa aaggaaagta tcttgttcta ggcgataacc gcttgatttc
     6661 aaaagacagc cgaatgtttg ggttagtcga caaagatatg atccaaggaa aagttgtttt
     6721 ccgttattgg cctctttcag aaattaaaat catgtgatga aaaaataaaa agaagctgtg
     6781 acatgttttt tgtcacagct tctttttccg aaaattcctt cttatttctt ggagttgctc
     6841 actttttttg caatctcatt ttaagctttt tggctgatgg ataagaaaag cgaagcgttt
     6901 ttgttcataa atagcttttc cccacgatgg aaggtatgac tagtagcaga gacaaagccc
     6961 gttttttcca atcgattatt caattcttct tggctaaagc cattatggac tttcggatga
     7021 ctaatttgtt cattcttgtc aaagtctacg atgatcaatt gaccgccagg agctaaaatt
     7081 tcataaagtt ttgtaagaat attctccgta tcaggaatgt gtaaaagtac aagtgataga
     7141 aggatgagat tagcttggac agcaggaaat tcgacagaag catcagcgtg gatcgttcgg
     7201 acatttttca gatctgctgc ttggatcttt tcttctgcca ttttcagcat agtttctgaa
     7261 gcatcggcaa tgatcagttc tttgaatctt tccgcaagcg gtaaactaac aagcccagtt
     7321 ccgcctccgt aatccagcag gactttttgg tggctgtcgc gcggtagata agtagttagt
     7381 tcttcgcgaa taatcttggc taatgcttga cggtcagggg aatcataatg gttagcgatg
     7441 gaatcaaaaa tattttttgt catcaaatag ctccttacgt aaaactttga aattttaact
     7501 tattatagca cgtgaaaaga aagaaagcat gctatactca attcaaaaag ggagaatcta
     7561 gaaagaatat gccgatatcg tttgtaaaag atagagagga aaaaggaaag tgtgtccgag
     7621 aaatattact agacttgcct gagtggtttg ggcttcctga atcaacggaa aaatatatcg
     7681 aggaatcaag taaactacct ttatggtgtg aaaaaagaaa agaagaatat ttaggcttta
     7741 tcactctttc acagacaagt gaagatactg ccgaaatcta ttctattgta tgggaataaa
     7801 aaaagactat catcaccaag gattaggaac aaaacttttt aaagaagcag aaggttacgc
     7861 agcaaaacat tacaaatatt tgcaagtgaa gactgtagac gaggggcact actctatata
     7921 tgatcaaact atttgtttct acgaatcatt aggattttca aggttggaag tttttccaaa
     7981 tttatgggat gaatggaacc catgtttagt tcttgtcaaa aaattggaac aaaagtgagc
     8041 aatagattta atactaatct gtgcttgatg aaaattctca ctgtaaaaaa tgctgaaaat
     8101 caagtataat accataggaa caaactggaa ggtgaaaaag agcatggcac aactattttt
     8161 taagtatggc gcaatgaata gcggcaagac gatcgagatc ctaaaagtcg cacataatta
     8221 tgaagaacaa aataagccag ttgtcttaat gacaagcggt attgatacaa gagatggttt
     8281 aggcgttgtt tcaagccgaa tcggactgaa acgtaatgcg atgccgatat ttgaagaaac
     8341 agacgtattt gacgtaattc agaaaatgga aagcaaaccc tattgtgtgc tgatcgatga
     8401 agcgcaattc ttaaaaaaga aacatgtcat tgcttttacg agaattgtgg acgaattgga
     8461 tattccggtt atggcatttg gtttgaaaaa tgattttcga aacgagttat ttgaaggatc
     8521 aaaatattta ttgctttatg cagacaaaat cgaagaaatg aaaacaatct gttggttctg
     8581 ccacaaaaaa gcaatcatga atttacatta tattgatgga aaccctgttt atgaaggcga
     8641 tcaagtgcaa attggcggaa atgaagctta ttaccccgtt tgtcgaaaac attactttca
     8701 cccatcaatg attacagaag gagaataagc atgtacgatc aattacaagc aattgaagac
     8761 cgttatgaag agcttggaga acttttaagt gacccagaag tgatttctga tacaaagcgt
     8821 tttatgcagc tttcaaaaga agaagcaaac acgcgcgaaa cagtcgaagt ttatcgtcgt
     8881 tataaagaag tagtacaagg aatcaccgat acagaagaac tgcttggtga aaaactggat
     8941 gatgaaatgg ctgaactagc aaaagaagaa ttatctgaat taaaaaaaga aaaagaagtc
     9001 ttagaagaaa aaatcaagat cttgctttta ccaaaagatc caaatgacga taaaaatatc
     9061 atcatggaaa tccgtggagc agcaggcgga gacgaagcag cattattcgc tggcgatctt
     9121 ttcaatatgt accaaaagta tgctgaagca caaggctgga aaacagaagt gatggaagct
     9181 aacgttaccg gaatcggtgg atataaagaa gtgatcatga tgatcaccgg agacaacgta
     9241 tattcgaaat taaaatatga aagtggtgcg caccgcgtcc agcgtgttcc ttcaactgaa
     9301 tcgcaagggc gtatccatac ttccaccgca acagttgtcg tgatgccaga agctgaagaa
     9361 gtagagatcg atattgaaga caaagatatc cgtacagata tctatcatgc ttcaggtgct
     9421 ggcggacagc acgtcaataa aacagcatct gctgtacgtt tgactcactt accaacaggg
     9481 atcgttgttg ctatgcaaga tgaacgttct caaatcaaga accgtgaaaa agcaatgaaa
     9541 atcttgcgtg ctcgtgtgta cgataagatc cagcaagaag cacaaagcga gtatgatgcc
     9601 aatcgaaaat cagcagtcgg aactggggat cgttcagaac ggatccggac gtataatttc
     9661 ccgcaaaatc gtgtgactga tcatcgtatc ggattaacta tccaaaaatt agatcagatt
     9721 ttagcaggga aattagatga gatcgttgat gcattgatca tgtatgatca aacatcaaaa
     9781 ctggaagcta tgcaaaatgg gtaaaatgac ctatcgagaa gtcctgtcac gggcttcttc
     9841 ttttttagag gatcatggga aagaagcata tagtatccaa tttttatttt tggaacgaaa
     9901 acaatggaaa aaacttgatt ggctccttca tatgaacgaa gagatttcag aagaagaaca
     9961 gcggttgatc gaaaccgatc tgcgacttct gcttgcagat catccacctc agtatttgct
    10021 aggatatgca gatttttatg atcatcggtt aaaggtaact gaagcaacac tgatccctcg
    10081 accagaaaca gaggagttag tcgaatggtg tttagacgag acgcctggtg taccgttaga
    10141 agtgatagat attgggacag ggactggagc aattgctatt agtttgaaag cagcaagaaa
    10201 aaattggcat atatctgctg tggatctttc agaagaagca ttagaagtag cgaaagaaaa
    10261 tgctcagaaa gaaggaacga aaatcagttt ttatcatgga gatacattag gaccagtaat
    10321 ggatcagcaa tttgatgtga ttatttctaa tccgccgtac atcagtcgga atgaatggga
    10381 actgatggat gaaagtgtcc ggtcattcga acctaaaatg gccctttttg ctgagaacga
    10441 tggattagct atttatgaaa aaatagcagt tgaagcacca agcgtactaa aatcaaatgg
    10501 aaagattttt ttagaaatcg gatttcgcca aggtgaagca gtaaaaaaaa tatttcagca
    10561 agcatttcca gataaaaaag tggcgataaa aaaggatctt tttggcaatg aacgaatgat
    10621 ccaagtttat ggttgaagtt gtggatgaaa aattttttat ttgttttttt attataaaaa
    10681 aacacttatt tgacaaggat gtaaacgact ttttggttta tcaacaagtt tatccactat
    10741 tgacaaatag attatccaca aacagtggaa aacttgagga taaactcaga caaaataaga
    10801 atgtatgaaa aaggggttgt ggataaactg tggaaacgaa acgatatgat gaaacagaat
    10861 tgcaagaggc agccgaagca ctgaaagaag gagaacttgt tgcttttcct accgaaacag
    10921 tatacggatt gggcgccaac gctcttttac caaacgctgt caagaaagtg ttttctgtaa
    10981 aagggagacc gcaagataat ccattaatcg tccatgtcgc ctcttttgaa caagtaaaag
    11041 agtatgtgga caactttcat ccggcagcag agaagattgt gaagaacttc tggcctggac
    11101 cattaacttt gatatttaag atcaaaaaag acactttgcc ctcagttgtg acgggaggat
    11161 tatccacagc tgcatttcgt ataccggata acaaaaaaac actagaagta atcgaacttt
    11221 ccggtgttcc gcttgtcggt ccaagtgcaa atacgtcggg aaaaccaagt ccaacgactg
    11281 cagatcatgt atatcatgat ctacaaggaa aaattaccgg gattattgat gatggagcta
    11341 cgcgaattgg tgtagaatcc actgttcttg atttaagtga tccaacagcc atgcctatga
    11401 ttttacgacc aggtgcagta acgaaagaac aaatcgaagc agtcatcgaa tctcctgttg
    11461 ctattgatca gcatcttgta aaagaaaatg aaacaccaaa agcccctggg atgaaataca
    11521 aacactactc acccgataca cgtgttctga tggtcagaga tggcgattgg tcaactgcag
    11581 ttcaatgggc aaaaaataag aagatccgtg caggtgtcat agcaagcccc gaaattgcgg
    11641 atcaagtgcg gacagatact gcagctgttt atatgtataa cgacaactca gtagaagcgg
    11701 cggctaaagg attgtttgca ggtctaagag gattagatga accaacactt ggattagatt
    11761 tgatttttgt acaagtgtat ccagaaactg gattggggaa tgcttacatg aatcgcttga
    11821 aaaaagcagc aggacaaaat tattttgaaa aatgatcaat tggttattgt cgcattcttt
    11881 tagtatgata caatgctatc aagtaaatct tctatcagtg aacgaaaaac taagcgtaac
    11941 taatcgtaat agaagagata cgaatatttg gcaatttgtt tttgatggga aaaataaaat
    12001 tgtaggaaaa taaaaaggag ctgttgtttg tggtagatta caaaacgttt gaccctgatc
    12061 tttgggctgc gatcgccaaa gaagaagaaa gacaagaaca taatttggag ctgattgcct
    12121 ctgaaaactt tgtctccgaa gcagtgatgg ctgcacaagg aagtatcttg acaaataaat
    12181 acgcagaagg ttatccaggg catcgctatt atggtgggtg tgaatttgtc gatatcgttg
    12241 aaaatttagc gattgaccgt gcaaaagaat tattcggagc gaaatttgcg aacgtccagc
    12301 ctcactccgg ctctcaagcc aacacggctg cttatctcgc tttggtagaa ccaggggaca
    12361 cgatcttagg aatggatctt tccgcaggcg gacatctaac acatggatca ccagtcaact
    12421 tcagtggcaa aacctatcac tttgttgctt acggtgttga tccgacaaca gaagttattg
    12481 attataatgt ggtacgtatt ttagctagaa aacatcaacc aaaattgatc gtagcaggtg
    12541 ccagtgctta cggacgaacg atcgactttg cgaaattcag agaaatcgca gatgaagtag
    12601 gcgcaaaact gatggtagat atggcacata tcgcaggatt agtagcagca ggacttcatc
    12661 ctaatccagt tccttatgca gatatcacaa cgactaccac tcataaaaca ttgcgtggcc
    12721 ctcgaggcgg catgattttg acaaacgatg aagcattagc aaaaaaaata aatagtgcgg
    12781 tatttcctgg tattcaaggc ggccctctag agcatgtgat tgcgggtaaa gcagtagcat
    12841 tcaaagaagc attagatcct gcatttaaag aatatagtga acaaatcatc gccaatgcaa
    12901 aagcaatggt caaagttttc aatcaagcaa ttggaaccag agtcatttca ggggcgacag
    12961 ataatcatct gatgttgatc gatgttcgcg agttaggtat aaatggaaaa gaagcagaga
    13021 gcattttgga cagtgtgaat atcactgtaa ataaaaactc gattcctttt gaaacactaa
    13081 gtccattcaa aacaagcggt attcgaatcg gcacgccagc aatcactact cgcgggttca
    13141 aagaagaaga tgctgtaaaa gtggctgaac tagttgtcaa agcattacaa gcaaaagatg
    13201 ataacgctca actagacgaa gtcaaaacag gtgtacgcga attaactgaa aaatttccgt
    13261 tgcataaaaa ataaaaattt aaattccatt agtaaacaga aaagttcaat gttgggcttt
    13321 tctgtttatt ttttgggaaa acggattctt tgacaatatc ggtgatgctc tctagtatat
    13381 gaggtgtaat tttagtcttc acactaaatc tcagggagga aaagtcatga ctgatcgtat
    13441 ctcgttagaa aaggaagcag tagcattcag tgatgcaaat gcgcctcatc cgcgtatcta
    13501 tgaactagca ccggaagaag gaagagtttt actagaagaa gtacaagaat cacctgtgaa
    13561 caaatattcc gtcgatattg aagacttgat ggtggatact ggcgaatggg gaaaactcaa
    13621 tattcgtctc gtacgaccag aagggaatat ggataaatta ccagttattt tttatattca
    13681 tggagcaggc tgggtttttg gcagtgctca aacccatgat aaattgatca gagaacttgc
    13741 ggttcgtacg aattccgtcg ttgtctttcc agaatatagt cgatcaccag aagcaaaata
    13801 tccaacagca attgaacaaa attatgctgt gttgcaacag ctgaataaat tagccaagtc
    13861 aaaaaatctg gatctcgaga atctaatcgt tgcaggagat tcagtaggtg gtaacatggc
    13921 tacagtcatg acgatcttaa caaaagaacg aaagggcctc ccaatatcca agcaattgct
    13981 gtattatcca gtcacaaacg ctgattttga tacagattcc tatcatgaat tcggagaaaa
    14041 ctattttttg acaagagaag gaatgatgtg gttttgggac caatatacga cgaatgaaga
    14101 agagcgtgct gaaatcacag cttcgccttt gagagcaaat ataaatgaac taaatgatct
    14161 tccttcagca atgattttaa cgggagaagc ggatgtatta agagatgaag gtgaggctta
    14221 tgcgcgcaaa cttcgtgatg caggggttcc tgttacacaa gtacgcttcc aaggaatcat
    14281 ccatgatttt gtcatggtaa atgctatgga tcagacaaag gcaacgcgtg cagcaatggc
    14341 attatctact tcttggatca agaaaaaaga ataagctttg cttgtccgca ggaaaacttc
    14401 ttttcaaaaa cggattttct taggaaaaat ggcgagtttg actagtcaat cacttgattt
    14461 tgttttacaa ttaaaaagaa acgacagaac aaaggagagc ttaaaaaatg ggcaaatttc
    14521 aagtgatcga tcatccattg attcaacaca aactaactat tattcgggac aaaaactgcg
    14581 gaacaaaggt ttttcgtgaa gtagtcgatg aaatcgctat gttgatggca tatgaagtat
    14641 cacgggatat gccattggaa gacatcgtga tcgaaacacc gatcactgaa acaacacaaa
    14701 aaacgttgtc tggtaaaaaa gtagcgatta ttccaatctt gcgcgcaggt attggaatgg
    14761 ttgacggtat cttggaattg attccagctg ctaaagtggg acatatcggt ctttatcgag
    14821 atgaagaaac attggaacca catgaatatt tcgtcaaact tccagaagac attgatgcac
    14881 gtcagttgtt tgtagtagat ccaatgttgg caacaggcgg atcagcaatc atggcaatcg
    14941 atgcattgaa agaacgtggc gcgtcgaaca ttaaatttgt ttgtttggta gcagcacctg
    15001 agggagtaaa agctttgcaa gaagcgcatc ctgatattga catctataca gcttctctag
    15061 atgaaaaatt gaatgaacat ggttatatcg ttcctggact aggggatgcg ggtgaccgtt
    15121 tgtttggtac aaaataagta aatacgaaaa agggtgtgac acaacttttg tcacatcctt
    15181 ttttggtcat ttttaacgtt tgatgcagaa cagctttttc acagctttcg tttgcatata
    15241 aaaaaaccat cccaaaaatg cagagatggt caaattcaaa tcttttttag ttctttgtag
    15301 ttaacgatta ctacaccctt actttgcgtc atcaagttcg cattttctgc agtttcatca
    15361 atctcaacaa cagcggaatt tttcaattgc tttgaaatcg ttccagtttg cgacttgccg
    15421 tgaattgtga acattacgtg agtattgatc ttgaattttt caccaatttc cgatggtacg
    15481 atagttgtat caaattttcc aaaagttgcc attttcataa aacctgcctt tcacatcaat
    15541 tataccacgt tttatgaaaa atatcatgaa attaaactaa ttttccatcg gactctttac
    15601 aatcagccaa atagagtata taatttcttt tggataaggc cagcttagct cagttggtag
    15661 agcaacgcac tcgtaacgcg taggtcgtag gttcgatccc tatagctggc attaaaagag
    15721 aagacagata tatcaaggat ttttaatcca ttgatacatc tgtctttttg tttaaaggaa
    15781 ataaagtatg ctttcttagc aatagagcat gtattatttc tataaaaatt taggagaata
    15841 aaacgactag caatatagct gtgaaatcaa atatcacatg aactgcccaa cttgtaatga
    15901 tcgagttgct tttaatggca gcttggttga ataacaatct tgccacccct tgtatcaaaa
    15961 ggatgtgtgc tacagtgtgt aaaatatttc cattatcata agtactgtaa tggataaatc
    16021 caaagatgat tgctgacaag aaactagcga caggaatgct gaacttacta gaaaaaatag
    16081 cgagaaaata cactgagaac aattcttctc caagtatcat gataggcaaa ataataaata
    16141 ataacgggct gaaagcctca tttattggat tgcctgttaa accccatttc aaaatttgtt
    16201 gcaaaaatac cgagactgcg gcactgatta ctacattgat aaaaaaatat ttggctatat
    16261 ttttgacagg agcgacaggt ttcgagaaca tttttttcgt atctttttta ccaaaaacaa
    16321 gtgtaaatgc tataaatcca gtgatagtta aagaaaaaat gctaataatg gatggcgcaa
    16381 atagcaaccc taacaaaccg agtacaacga caatagtgcc caatatccct tttttaagtt
    16441 ctattttttt tcctaaaatg ttattcatga taataactcc tactattcag tttttgaaga
    16501 tagagtcagt atacatgaag agttaaaata ggcaatgttt ttttatggat atttgtattg
    16561 ctaaaaattt atcttatata ttcattttga ttatactact tttatttgta aaaattttct
    16621 agcaaaacca agtgcttgca actgattaag cgtacaattt tttagatgtt ccgagcgtac
    16681 ttcaatcatt tcaaaggagt tgctacttaa atcgatattt tttaaatcag tatcaaaaaa
    16741 gctagcattt tctaaatcat tagtagaaaa cattatttta gtgagggaac agtctacaaa
    16801 atctgcgctt gataagacag agtgctcaaa ggtactttct tttatgtttg aaccagataa
    16861 atttacatag tccattttac aattctcgaa taacgtgttc ccaatataac tttccatcaa
    16921 tttagtacct gttaatttac aattgacaaa tttcacgcga tgcaacatgc tttttgtcaa
    16981 tatacagcct gaaaaatcac agttttcgaa tatacagtcg gtaatctcca ttctttgaaa
    17041 atcattggaa gcaaaaaatg catacttgaa aatagatttg taaataattg gatggcagat
    17101 tatttggttt tcgatgacct ctttttgtga aaggatatta tacagttcaa aatcttcgtt
    17161 ttcatagata tgaaaaaagg atcctttatt caagcttgct tcattaactt taggaaaaat
    17221 catatttaat tacctccatt ttcataaagt catattaaca aaagctaaac caacttacat
    17281 tagcagaaaa tgagataggt tttcgccttt ttcgataacg atagtgtcgc tatctcttga
    17341 aacgttctta gaattgccct gagagacaca aattaggcgt aattctaata aaaatcttag
    17401 aataaaaatt tttactgatt taatttagaa tgtttttaaa ataaaaatcg ttgccataac
    17461 agtgtattga aaggttttaa caatccagta atgatttaaa aaaatctaga tgtagcgtat
    17521 actttgttta tagaatgtag tgggaagaaa tatatttttg ggaggaaatc gtgatgtcta
    17581 aaagtgaaga aatagaaaac atggtactaa aattatatga tctcgtttta aatccagaga
    17641 tcaaagaaaa ggaacgtata cttttgatag acgcaaagac aggcttagaa aaaggtcagt
    17701 attatcctaa agtcataaat aatttggaac gtagtttacg tccgttagca atcaggggag
    17761 aattgtctaa accagtatca ccgttttata tggagatatc aacaatagga aaatttgaaa
    17821 aagaattagg tcgaggaatg gcttcggcac cgattacttt tgggcattta taaaaacgag
    17881 ttattcatct caatgaatta aaaagtatag tcaatctcat aatgcaggct aggttcattt
    17941 atttgaacag agtctgtatt tttttattct aaataaaga
//
LOCUS       DACDUU010000063        17179 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.063, whole genome shotgun sequence.
ACCESSION   DACDUU010000063 DACDUU010000000
VERSION     DACDUU010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.063"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..300
                     /locus_tag="IWL30_002126"
     CDS             <1..300
                     /locus_tag="IWL30_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9706859.1"
                     /translation="DGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKEN
                     RKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(724..894)
                     /locus_tag="IWL30_002127"
     CDS             complement(724..894)
                     /locus_tag="IWL30_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9706860.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1088..1267)
                     /locus_tag="IWL30_002128"
     CDS             complement(1088..1267)
                     /locus_tag="IWL30_002128"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706861.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            1654..2766
                     /locus_tag="IWL30_002129"
     CDS             1654..2766
                     /locus_tag="IWL30_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9706862.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(2844..3137)
                     /locus_tag="IWL30_002130"
     CDS             complement(2844..3137)
                     /locus_tag="IWL30_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAP9706863.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3305..3619
                     /locus_tag="IWL30_002131"
     CDS             3305..3619
                     /locus_tag="IWL30_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAP9706864.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            3640..5619
                     /locus_tag="IWL30_002132"
     CDS             3640..5619
                     /locus_tag="IWL30_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9706865.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            5632..5958
                     /locus_tag="IWL30_002133"
     CDS             5632..5958
                     /locus_tag="IWL30_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAP9706866.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            5974..6492
                     /locus_tag="IWL30_002134"
     CDS             5974..6492
                     /locus_tag="IWL30_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706867.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            6492..7844
                     /gene="celB"
                     /locus_tag="IWL30_002135"
     CDS             6492..7844
                     /gene="celB"
                     /locus_tag="IWL30_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP9706868.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(7928..9433)
                     /locus_tag="IWL30_002136"
     CDS             complement(7928..9433)
                     /locus_tag="IWL30_002136"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAP9706869.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLEFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(9525..10115)
                     /locus_tag="IWL30_002137"
     CDS             complement(9525..10115)
                     /locus_tag="IWL30_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9706870.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10294..11940
                     /locus_tag="IWL30_002138"
     CDS             10294..11940
                     /locus_tag="IWL30_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9706871.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYFQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            11989..12708
                     /locus_tag="IWL30_002139"
     CDS             11989..12708
                     /locus_tag="IWL30_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP9706872.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13187..14134
                     /locus_tag="IWL30_002140"
     CDS             13187..14134
                     /locus_tag="IWL30_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9706873.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14146..15066
                     /locus_tag="IWL30_002141"
     CDS             14146..15066
                     /locus_tag="IWL30_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9706874.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15107..16564
                     /locus_tag="IWL30_002142"
     CDS             15107..16564
                     /locus_tag="IWL30_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9706875.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            16909..>17179
                     /locus_tag="IWL30_002143"
     CDS             16909..>17179
                     /locus_tag="IWL30_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9706876.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYN"
BASE COUNT         5394 a         2926 c         3454 g         5405 t
ORIGIN      
        1 gatggcttag caccaggagc atatcagctg gtagaaactc aagcgccaac ggggtatgaa
       61 ttggatgcga caccagtaac tttcaagatc gaaaaagaac aagaagtggc gatcttttta
      121 acaaaagaga atagaaaagt tgctgatagc tcagatataa gaggaaacaa tccaacaagc
      181 agtggaaata agcatcttcc aaaaactgga gaaaccatca ttagccagtt tatattgtca
      241 atattgggtg tgctacttgt tttcatcagt ataaaattta gaaagaaacg aaatatatga
      301 gctaaaataa aaaattgaat aagcatagtt tttaaaatca actagtaccc atcaattgaa
      361 tggaaactac cgattttaga aggggtatac aaacaaatat tagtatcgga gtagagaagg
      421 ttgaacccaa ccagtaaata agtaaagaca attttgtgct gcgttgagaa gtgaaaaata
      481 aacctttgta gtaatactag ttaataaaaa atatacaaaa acactgcgaa aatcattctt
      541 gagtttcgca gtgttttttg cctaatgact caagtttatt gggtaatact ttgattttct
      601 ttctacaaat aatcagcgat cagttgtaat tttttctcta gttttaatgg ctctttgtca
      661 ataaggagaa aaacaagtcg ggtcttttgt ctcggaaagc ttcaaccaac tcgtgaataa
      721 aggttacgcc tccttgaaag gcgtggaaaa accaagtaat cgatcaatca ccaatttcgc
      781 atttggtaga acatgtttgg tgagctgaaa gtaggtggcg ttcatgtctg taactaagaa
      841 ttctatttct ttaggattag tgcagctaag gaaatagttt gttaatcgag gaattttttg
      901 tgtaggtaaa ttttctcgat taatttttct gtctcgccat ccgcggaaat aaaactcagt
      961 ttatcctcaa cggaaacgtg agaacgaaat tcacaaccct caacactcaa gacagaattc
     1021 ttttggaaga ctttggtaaa tagcttctca actctttcaa tgtgcgaata acggttgtca
     1081 aagataccta acaacttttc gaaataaaaa ataacgacac tttttcagtc agtaaagaag
     1141 tgattttatg tctgacatga tttgaaatcg agtgccttgg acggataaaa taactttgag
     1201 cgttccaatg gcttcgacag tttttacagg tatagcgctg cttttttagg cgtataacca
     1261 ttggcatatg attatattat tcaaagtgga caatggtttc cttcttcttg tttttcacca
     1321 ccattaattt cctatttcca tctaccacag cagaaccaca atttctacag acacatggag
     1381 cagggaagag aactgcagca acgatcaatg tttttttctt ctgtaaggtt tcgtaagaga
     1441 cctctgtaat catcaaattt ttgtctattg atctcagaat ttttttgata gaaccattca
     1501 taaagcatat cgttctctca gttgtttatt ttgtggtgat ttaatcatac aagaaaacga
     1561 tatgtttttt tataaaaaca tgaataggaa aaatagttga caagaaaatg acaacgccat
     1621 atgatgttcc atagtattta caacctgtca tagatgactg taaaaaataa aagtaaaaag
     1681 aaaggacgac aacaggtgga tttttatcag tctctacaac tagatccgtt tattttaaaa
     1741 caaaaaatac gagaagcagc ttctaaaaaa gaaaagtgca tgtatatttg ttcattgttt
     1801 ttacgatcat tattcatcgt attgtttgcc atttgtttta tcattataat tacaacatta
     1861 tttgaatcga agcataaacc atacgctgtg gttttattct gtatgttgat gagtattcgt
     1921 tttgttgact ttggttataa gatttcccat tcgatcatag gtttagcaat cgtcatgttc
     1981 tcactattag tagcaccgta tgtccagctg atcaaatggt cagttatggg catgttgatt
     2041 cattttattt tactaagcag catattaatg gcaaccgcta gtgatcccaa aatggggaat
     2101 gcttctttgt acggcttttc atatctcttt attgtttatt cgttacctaa agattcgttg
     2161 aacaagaatt tctttacaca aacaagtagc ttattatttt tgtttttttg ttggttttca
     2221 gttcttcttt acagaaaaca tcgagaaaag aacaaagaca aaagcttgtt taaagagatt
     2281 tttttgaaag gcatgtattc gcaggaaaaa atttggatgc tcatctatgc atttggcatt
     2341 agtcttttga tcgttgcagg agagtatgtc ccttttcagc gattgatgtg ggcagggttt
     2401 gcattttctt ctattgtatc tagctatgga ttgatgtcga ttggtttcaa agaacgagca
     2461 gtagaccgaa tcattggctc attgattgga tgtgttttat ttattggtat cagccaattt
     2521 attcctttta actgggtcgg tatattaggt ggtctagctt taggtgtttg ttctacctat
     2581 agatataaaa cagtttttaa ttcttttggt gctttggcaa tcactgccag tttattcggg
     2641 gttccaggag cagtaacgat ccgcattttt gagaatatcc ttggggtatg tttaggaatc
     2701 atgtatattg gggtgactga aatacttatc cgaaaaataa gagaaaaaca cggtttgaat
     2761 cattagatga gatcgaataa aaaataagga aaaagtgaag atacacatgg atgataaatt
     2821 gctatcattc catgtgtatc tttttacggt aaatcagtga aacgttcgac tttcattttt
     2881 aatttatact ttttacggag ttcgtttatt tgagccatca tttcggattt gtgatgcttt
     2941 tcgattgctt cttcatcttt ccaacggtcg atcagtaaaa gagattctgg atcttctagt
     3001 ggaaagtaat agtcatattt ctcgtttcct tcttctgcac gaatcgcttt gacaatgttt
     3061 ctttcgacca tttcttctgc aaattttctg gcttgtccat ttgtacttgt atacaaaata
     3121 ttcactgtga tactcactaa atcccctcct gaaaaaattc tatcactagt atatcacggt
     3181 gccagaaatt attattcaag atatgataca cattttttcc gcaatgctac ggaaatatat
     3241 attttgaata gaaaacgctt acaagatatg cttggtttat aaaaataaaa ggagtgattg
     3301 atttatgaaa aaagcattga ttatttgtgc ggcaggaatg tcatcctcga tgatggcctc
     3361 aaagacaacg gatttcttta agcaaaaaag agaagaggta ctcgtagatg cagtttcagc
     3421 tacagaaggc gataatatga ttaagaatag tgattttgat ttatttctga tcagcccgca
     3481 aacgacgatg tttttggata aattcaaaaa attaggagaa tctgttggaa agcctgttgt
     3541 tagtatccct ttccaagcgt atgtcccaat ccctacaggc atccagaagt tagcagaggt
     3601 gatcgaagag aacatatgat ggaataggag tggatgataa tgaaacagaa ggaaaaattg
     3661 ctgttgagat atttgattga tcacaagaag gagtatgtca ccagtcaacg tttagcttct
     3721 gaattgttat tatctgatcg gacaatccgt aattacttgc agcggatcaa agagttagta
     3781 gaaaaaaacg gaggtaagat cattgctaaa ccaggttatg gctaccaact gcatatttta
     3841 caaagactga catttgactt gtttttgagt cggcaagaaa tagtacctgg atatgctaga
     3901 gaaccgcaag gattttatga gtccgaagat cgacaaaagt atatattgaa ccaattgctt
     3961 ttggaagatg ccattctttt tatggatgat ttggcagaag agctttatat cagtcgttcc
     4021 agtttgacaa aggacatgca agaaatcaag gagcgattga ttccatattc actaaaaatc
     4081 gtttctaagc atggtcaagg gacatggatc gaaggggaag aaagaaatcg acggcatttt
     4141 atcatggata ctttttttgg aaaccattat ggaaattcat tgaaagaata tttagggcaa
     4201 agccgttttt tcccagacat cagttttgaa gagctagtca ttatcattct cgatgaaaca
     4261 agagaagcta agttaaaagt ttcggatttt atcatacaga atctgacttt acacttggct
     4321 ttggggatca aacggctccg agaagggttt gaaatcaagg aattaggcat cgaacaagaa
     4381 gtttctaagc gagtagagta tcaagtggcg cagcggatcg ttcaacgtat tgaggtaatc
     4441 gcaggtgtat cgtttcccga agaagaaatt gcttatttga cattacatct catggctaag
     4501 tcgaatcaca aagaaaatca aaatgatcag gaactatcaa aggaattagc gaaagttctg
     4561 aaaagattgt ctcaggaatt tcatttttca ttagtagagg atctccagct tttcaatgga
     4621 ctactcgatc atttgtcacc gatgatgatt cgattgagcc gaggtattcg cattgagaac
     4681 ccattgactg aggagataaa gaaagaaaat cccaaagtct ttgacgcagt aaaaaggcat
     4741 ttcagcaaca tgccggcttt gaaaaactat accatcaatg aagatgaatg ggcctatttg
     4801 gctttgcatc tcatggcggc attggaaaaa gaaagagctg cgcataaact acatgcattg
     4861 attatctgtg caacgggtta cggcagtgcc caactattaa agaatcgcgt agtcagtgaa
     4921 ttcggtaaaa atattacagt ggtaagtgta aaaggctatt atgagatcaa tgaaacaact
     4981 ttaaaaggaa tcgacctgat tatttcttct attgatcttt cgacaatgtt tttcaaaatt
     5041 ccagttttac atgtcagtat ttttttgaat gaagatgatg ttcgtaaaat acgaaaggta
     5101 gttggagaat ctatacccta tcattcctca gatagaccga taccagcatt ttctctacag
     5161 caaaaaaagc agatttacac cgaacagttg tctgaaaaat ttttcaagac ttatacgtac
     5221 gaaccgaaaa aggaagaaat tcttcatgat ttgctgcaaa agctgagcat aaatgaagaa
     5281 gaaggatata taagagaaat gcgaaagcag atccagcaac gtgaaaatat gggacaaatc
     5341 atattcagtg atacagtggc agtcccacat cccgctgttc cagttggaat cgaaacaaag
     5401 gtagccgttg cattgattcc ttctgggatg cagtgggatc aatatcaaga aattcatttt
     5461 gtttttttag tttcgccttc ttatgtcgaa aatgaaggaa ttacggtcgt gactaaagca
     5521 atcgttaggt tagttgacca gctaaaagta cagcaggaaa tcttagctga acctattttt
     5581 gagaatttca gcagtaaatt tatcaaactg atcgaataag gaggaaaaca gatggctgaa
     5641 aaaataaaca gtgaaacact acaagtgact tcttttgata tcattttcca tagcggaaat
     5701 gcacggacac tgatacatga agcatttcgc tcgatgcgta aagagatgtt tgaagaagta
     5761 gaagagaagt tgcgacaagc aaatgaagag attcttcatg cgcatcagtc acaaactcaa
     5821 cttctgaaag aatatgccag cgggacaaaa atagaaatgg aaattatcat ggtccatgca
     5881 caagatcatc tgatgacgac gatgacatta ttggaagttg cacaggaaat gaattatttg
     5941 tatcaaaaaa ataaatagaa aggaaggatc attgtgcagg atacagtaaa aaggaaaatc
     6001 gaattagaaa aaaagcaact tagtttgaaa aacatgtatt tcaatcgtta tctaatcgta
     6061 cgttacctga ctgccttttt ctttttcatg aacatgcaat ggatgattct gcttctatca
     6121 gctaaaagtt taggaagtag tttaccgatg attctgattt tagctatttt gcctgcagta
     6181 ggagaacaag taaagcttta tcgaaaacac caaacgaacg ttccatggac aaagtggtat
     6241 tttttgttcc agagtgtctt taatttatta gtgattccag tgttgtttac ctcaggattt
     6301 acattcttgt atccttttat ggcaaataac aatggaggac agttatttgt tttcattctt
     6361 gttgtaagcg gtatttttgc cagtgcattt atacagtatc gtttaaagaa aatttcactc
     6421 aaccaagacc aacagtatat ccgtattaaa caatatgaaa aagcgttata tttaggaaag
     6481 gagaataact aatgtttgat tttttgcaga aatacctgat gggacctatg gggaaaattg
     6541 cgcaacttaa aatcgttcgg gctgtcatgg cagcggggat ggcttctatt cctttcacga
     6601 ttgtaggctc gatgtttttg gttttaaata ttttgccatt accgttccca tttttagaag
     6661 gattttttaa tgcaactttt ttcaaagtta gtgacttgta catgatcgtc aatacaatga
     6721 cgatggggat tttatctatt tactttgcca ttgtgtttgc ttatgaatta acgtcaattg
     6781 aacgagatga acaaaaatta aatgttaatc cgctcacggg ggcattacta tctgtttttg
     6841 cattctttat gtgtatccct gaattgatca tgactgatgg taaaatcagc ttgattgctt
     6901 ctatcaatga gacagaaact gtagtaaatg gtattaggat gggctctttt gtcgaacgtt
     6961 taggaacttc aggaatcttt acagctatta ttatgtcgat tattgcggtc gaattgtatt
     7021 gtatgtgtgt caagagaaat tgggtcataa aaatgccaga tgttgttccg ccaggagttt
     7081 ctcgttcttt cactgctctg atcccaacat ttgtcattgc gtttgtggtg atgatcatca
     7141 atggattatt agttgcacta ggaacggata tcttcaaaat gattgctatc ccatttgggt
     7201 ttgttacgca gttgacgaat acatggcttg gtgtcatggt gatttatttc ttgatccatg
     7261 cattgtggat cgtagggatc catggagcga atatcatcac ttctttcttg accccgatcg
     7321 tattagctaa tatggcagct aacgcacaag gcgcaaatta tccgttggca ggagaattca
     7381 acaattctta tgtaaccgtt ggtggatcag gtgcgacttt aggattgatc atctttattg
     7441 cattcctcgc gaaatctgat cagctgaaag tgttaggaaa agcctcactt gtaccaggat
     7501 tattcaatat caatgaacca atcattttcg ggatgccaat cgtctacaat ccctatttag
     7561 cggttccgtt tttcttagct ccgatggctt ctgctacact cgcttatttt gccattcggc
     7621 tggaaatcgt caaaccgatg cttgcacaga tgccgtggcc ttcaccagtc gggatcggag
     7681 catttgttgg tagtggtggt gattggaaag ctgctgtgtt agcagtcatt tgtgcaatca
     7741 tcgcattcat tatctggttc ccatttatca aattctatga taataaatta ttaaatgaag
     7801 aaagagaaaa ggcagcagaa ttagctagtg aaaagttaaa atgatttcta agaaaaaagg
     7861 aactgtgaca aaaaatcaca gtcccttttt ttcttagaaa tgggaaacca ctagtatata
     7921 aataccatta ttcttcaatc aaccgcttca cgattgcata atccccttca taaggttcat
     7981 ctacaccatt gattttttga tagggatcca cgccttttcc cgcaataacg actgaatctt
     8041 gcggagcgct ttctgctaaa gcgcgtcgaa tcgcttcagg acgatccatt tcataatgaa
     8101 cgatcaaatc tggattagta attgcttcat tgatttcttc cgcgattttt ttcggatctt
     8161 caaatgctgg gtcatctgcc gtcaaaaagg cgatgtcagc tgtttctgat agcacttttc
     8221 caaaatcatg tcgacgggag attgctttgt ttcctgggct gcctaataca acaattacac
     8281 ggccatctgg atgttcattt ttggcaaatt ccagcaatgt tttcaagctt agataattat
     8341 gtgcatagtc cacatagaca tgcgctccat tggattggat caattgatcc attcggcctg
     8401 gtacacgagc ttcttttaat ccgtcttgtg catcttgact ggaggcacct gttagtgctg
     8461 cggcaatgat cgcactggct gcattttctt ggttgaatcc tccagccagc aaaatatcat
     8521 aagcagtatc gcagatagct aagcgatctt catgagaaga taaagtaaaa ccgtgagtac
     8581 ctttatctga acggatcact tgataattgc tttcttccga acgaccataa gtgatcgttg
     8641 gaatctggaa tagatcggct gtttccctaa gtaaatcaaa atagtcactt tcatgattta
     8701 aaacaactgt ttttgaattc aagatcaatt gtcgcttgca atagaaatag tcatcaaatg
     8761 tcggatgttc gatcggactg atatggtcag gcgagatatt caagaagatt ccgacatcta
     8821 aagtcaagcc gtatacgcgt tgtgttttgt aagcttgtga agaaacttcc atcactagat
     8881 gagtcatacc attttcaact gcttctgaca tcatacgata taaatctaat gactcaggcg
     8941 ttgtcaaatg ggatttgaaa tatgttttgc catctaacgt tgtattcata gtagaaagca
     9001 atgctgtttt tttatttgtt gtgtgatcca agattgcttt tgtaaagtac gcagcagttg
     9061 ttttaccttt tgttccagtg aatccgatca ctttcaattt attttgcgga tagtcgtaaa
     9121 aagccatagc aagaagcgcc attgctttac ggatatccgt tacgatgatt cctactgctg
     9181 aattttcgta tggttgttct gatacataat attgaatacc ctgttcgatt gctgaatcca
     9241 aatattcttc tttgaaattc attcctttac agaaaaacaa agtagaagca tcagcttttc
     9301 ttgaatcata acttaactga agaaattcag ctttttctgg tacatcaaga tgccagcctc
     9361 ttgttgaaac aaattctttt aatagatttt cttttaatag taaatgacgg atgtcttcta
     9421 aagagaagct cattgattga tccctcgctt tattcaattt cttggtttat tatagcacac
     9481 tctttttaat atatcgtttg tttccgctat gacaaaatcc agctttattc aaattttctt
     9541 gacacagggg acaagcggat gagtattttc ataaacaatg aactgactgt aatagatagg
     9601 agaacgaata gtgtctccaa caatccaaaa acataataat tcaaaaagac gataagacta
     9661 ttggtaagta aaagtccttg cggcattgga agccgaaaaa atttgtggaa aataatcggc
     9721 ggtatcgtta taccgccatt tgaggccccg actgtgtaca atgtgtaaac cccaagactg
     9781 aacaaaagac ttcctgaaat cagtgaaaag aaatgtgagg agatcatttc cacttttgga
     9841 acaatctcta aaacaacagg aaaaagcaga cttcccgcta cagtcttaaa aaatactgat
     9901 tttcctaata ggacagcggc tagaagcagc atcataagat tgatgctcca aacagtaact
     9961 gagcgttgga tagaaaaggc cgtttcaact aaatgtccga tggcgcccac tccggcggaa
    10021 gctatatcgt gagggagcaa gaaccaattc aatccaactg tgataaagaa aagacctaaa
    10081 aatataacgg gtagtttttt tatttcttgt gtcattgata tctcctataa atgttagcca
    10141 taaaaggctg tatatttctt tactttacca aaaaaacgaa accgaattca aaaaaacaat
    10201 tgttcttata aaagttatat aaaatcgatt gccagtttct gttttaccaa ttaaaatgat
    10261 agtaatgttt tgtagaaagt tggaatctga ccgatggata atcaaagaaa accagcatta
    10321 gatcaattaa caccgcaaga aaaaatggca cgaggctctg cttggatgac cgccagtaat
    10381 atcatttcaa gattgctagg cgctgtctat atcattccct ggtatgcttg gatgggcgaa
    10441 aatgctaagg ctgccaatgg attattcaat atgggataca atatatatgc attgtttcta
    10501 ctgatctcta cagcaggaat acctgctgcg attgcaaaac aaactgcacg ctataattca
    10561 ttgaatgaat atggcacttc acgacgatta tttatccgtg cattacagat gatgggcggg
    10621 ctaggcctgt tgtttgcttt gttcatgtac atcgcctctc cttggctagc tcatgcttct
    10681 ggtggcggag aagaactgat tccaacgatg cgttctttga gtatcgctgt attggttttt
    10741 ccttgtatga gtgtgatacg agggtatttt caaggcaatc aagaaatgat gccttatgcg
    10801 ctgtcgcaga ttgtagaaca ggtggcacgt attttttata tgctattggc tacattcatc
    10861 atcatgaaag tcatggatgg tgactatgtg acggcggtca cgcaatcaac tttcgcggca
    10921 tttatcggta tgctagcaag tattcttgtc cttttgtatt ttttgaagaa acatcaagcg
    10981 tatacatcgg cgtttattca ctacagcgaa aacaaaataa atattgcaac gaaggaattg
    11041 ttgttagata ccgttaaaga agcgattccg tttattattg tgggttcagg ggtaacgatc
    11101 ttcaaattag tcgatcagtt cacttttatg aagatcatgt ctgcatccac cgagtattcc
    11161 aatgcacagt tactggatct tttttcgatt tttagtgcca atccagataa gttgacgatg
    11221 gtagttattg cattggcaac atcgatcgcg tctactgggt taccgttgat tacagaagct
    11281 gttacgttga aagatcgagt aggtctggca aaattgacaa gcggaaattt acagttgttt
    11341 tcatttttta tgtttccggc gacatttggt gttatgttac ttgcctatcc gctaaataca
    11401 ttattttata caccggattc tctaggaagt aatgtactga tccaagcaag ttttgtagga
    11461 ctcttcctag gactatatat gctagtttcc aacatgctgc aaggaatgtt tgaaaacaag
    11521 gcggcaatta attatttggt tattgggttt ctagtgaaac tccttcttca atacccagcc
    11581 attcgtttat ttgaagtata cggcccttta ttggcgacaa tgatcggatt tgctgtgtct
    11641 tgtaccttga tcttaaagag aatccatgaa gtagcccgct ttaaaccaag ttttgtctgg
    11701 cgcaggacat tgctgatctt tattcttacc ttgattatgc ttttggcttc gtggatcaca
    11761 aagatgattt ttggtgcttt tttaaacgaa gatagcaaat tccagtctct attgttgatt
    11821 cttgtagtag caggtatagg ggctttagtg tatggttatt tagctttgaa aatccgcttg
    11881 gcagaaagac tactaggacc aggaatagcg cgtttacgac ataaattaaa aataaaataa
    11941 gtgaatcaga agagctagat tgatgctctt ttgaaatggg ggaaacgcat gcgtttagat
    12001 aagtttttag cagatatggg acttggcagc cgcaaagaag tcaaaggatt gatcaaaaaa
    12061 ggaacgatcg aagtcaatgg acaagtggtg aaaagcgata aagcgcaagt aaatgaattc
    12121 gaagatgcgg ttacttattt aggagaaatc attacgtatc aaaaagattt ttactacatt
    12181 ttgcataaac ctgcaggagt catttctgct actcaggata atcatgatca aaccgtaatc
    12241 gatttattgg aagaccagga ttatcgggaa gatctttttc cagtcggccg attagataaa
    12301 gacacagagg gcctcttgat tttaacgaat gacggggtgt ttgcccatca gttactatca
    12361 cctaaaaaac atgtggagaa agaatatcta gctgaggtta gcggtataat gacagaagaa
    12421 gatatccgtt tatttgcaga aggactgatt attgatggcg atgagcagac attgccggca
    12481 aaactccaaa ttgatcaagt agatcaagag acagaaactt caattatccg tctgattttg
    12541 catgaaggaa agtttcacca agttaagcga atggtcaaag cagtaggaaa agaagtctta
    12601 tatttaaaaa gaatccgtat gggaaaattt tggctgccta aagatttgaa taaagggcaa
    12661 tatcgaccca tgaccaaaga agagctagaa caagtgagag aaaaataatt gaatggatag
    12721 ctaaaagaat aagcagagta gttgtagcgc catctgaaaa gcagacttgt ccgtctgact
    12781 ttcggatggc ttttttgtga aatttcggct tatttccgta gaagctactt ctggaacacc
    12841 gtttgttcgg aataagggac tgtgacaaga cttttgttac agtccccatt gttccttgtg
    12901 actcagagtt agatagtaag gacacgatct ccctaatact caggccaaaa cgctgaattc
    12961 acaacctctg ataaacggtg ttcaatcagc tgaccctcgg tattagctca aaaaacggca
    13021 aaatatgagg aactattttc ccgttttttt ctctaatact caggccaaaa cgctgatttc
    13081 acaacctctc ctatactttg tttcattttc cctatacttt gtttatttat tctcattatt
    13141 gaaagcgcta taattacgtt gcgaatgaat cgaaaggggt ttttagatga aaaaacgaaa
    13201 ggggttcaat cattttttta tgaatctatg gcgttataaa gcacttgtcc tgatggctgt
    13261 accgggtatg ctttggatga tttttttctt ttacatacct gtaatggcga atgtcgtagc
    13321 attcaaaaat tttcatattt cagcagatgg gttcttggct agcttgaggg aaagtccttg
    13381 ggtgggttgg gaaaatttca aatttttatt ttcttcggat caagcatttc tcatcactaa
    13441 aaatacaatt ttatataatg tagcatttat ccttttgaat ctgctgatct cagtcgtatt
    13501 tgcaatcatt atgagcgagt tgagaaataa acgactcgtt aaagtttatc aaacgatgtc
    13561 cttactacct tattttctat cttgggtcat tatcagttac tttgtttacg cttttttaag
    13621 tcctgacaaa ggaatattca atcagtggat cactggacat ggaggagaag ctgtcaattg
    13681 gtataatgaa ccgaaatatt ggccgtttat cttggtgttc attggtacat ggaaagggat
    13741 tggttataac agcattattt actttgcatc tgttatggga atcgatccga cttattatga
    13801 agctgctatg gttgatggag ctagcaaatg gcagcagatc aaacatgtga cgataccaca
    13861 acttattcca ctaatgacga ttttaacgat tttagccgta ggaaacattt tccgagctga
    13921 ttttggcctg ttttataaca ttcctaggaa ttccggtgca ttatatgatg tgactcaagt
    13981 tttggatacg tatatctaca atggcctgac atccacagga gattttggta tgacggctgc
    14041 tgcaggactt taccaatcag ttgtcggctt tgtcttgttg atgatcacaa atacgatcgc
    14101 ccgacgtttt gatagcgatt ctgcattatt ctaaggagga aaaaaatgaa aaaaaagaaa
    14161 gtggagagag tccaagtccg ctctttcaat aagacggcta attttttctc taatataatg
    14221 attgcactct ttgctatttc ctgtattttg ccatttatat ttgttatcgc catctctttt
    14281 accgatgagt ccatattgtc gactcacggg tacagttttt ggccagaaaa gttcagtata
    14341 tttggttata cgtacttgtt cgaccagatg caggataaaa ttttccaagc gcttttcgtc
    14401 actgtacttg ttactgtttt aggtacactg atcaatagca cggctacttc tttatatgct
    14461 tatgcgattt cacggtcaaa ctttccattc cgtcgttttt ttaccgtttt ttgtttgatt
    14521 acgatgttgt tctcaccagg gatggtggcc aattatcttg ttatgacaaa tcttcttcag
    14581 ttaaaagata cgatttgggc attgatcttg ccaatggctg tcagtccctt caatattatt
    14641 gtgatgcgca cattctttaa acgttcggtc tctgattcga ttattgaatc ggcacggatc
    14701 gatggagcta gcgaactgcg aatatttgtt cagatcgttt taccgttagc tgttccaggt
    14761 attgcgacaa tcagtttatt tgccgcattg ggttactgga atgactggtt caatgcttta
    14821 ctatacatcc aagatgataa gctagttcca ctacagtatc ttttgatgaa aatccaaagt
    14881 aatattcaat atctgacaca aaattcagga gcaggaagcc aattgacagg cggattagcc
    14941 tcagttccag gagaatctgc cagaatggcg atcgtagtca tctcgacgtt gcctattgcg
    15001 atcagctatc catttttcca gaagcatttt gtcaaaggtc taacaatcgg cggagtaaaa
    15061 gaataaagaa acaaaaatat aaatcataaa attaggagga agaaaaatga agttatggaa
    15121 gaaactagcc tttacaggtg tttcagcgtt gttgttggga acattagcag catgcggagg
    15181 ttctggttct aaagaccagg cagaagctag caattcggac actttgcaga tgtatcaaat
    15241 cggagataaa ccagataatt tcgatcaatt gatggaagta gcaaacaaac gaatcgaaaa
    15301 agagatcggt gtaaaagtca atatcaatta cattggctgg ggcgattacg aaaagaaaat
    15361 gaacgtcatc atttcttccg gggaaaatta tgatatcgcc tttgcgaaca actatgtttc
    15421 caatgcccaa aaaggagcct ttgctgattt gactgaatta gcgcctaagt acgctgaaaa
    15481 agcttataat gatttagatg aagcatatat taaaggaaat ctggtcaatg gaaaattata
    15541 tgctttccca gtcaatggaa atgtctttgc tcaacaagta cttactttta acaaagcttt
    15601 actagataaa tacgatcttt caattgatgg aatccaatct tacgctgatg cagaaaaagt
    15661 cttgaaggaa ttccatgaaa aagaaccaaa cacggcagct tttgccattg gacaaggctt
    15721 caaagtccaa gggaactttg attatccttt agggaatacg ttaccatttg ctattgatct
    15781 ggatggagat gattcgaaaa tcatcaacca atacgataat gaaacgtata tcggactgtt
    15841 acgtacaatg catagctggt acaaacaagg actcattcca agtgacgctg caacgagcaa
    15901 taaggattat ccactagaag gaaatacttg gctaatgcgt ggagaaacac aaggaccgta
    15961 tgattatggc gatacaattt tgacaaatgc agcaaaacaa gaacttgtct caaaagcaat
    16021 tactgttcca ctaaaatcta cagctcaagc acaaatggca aattttgtcg tatcaaatac
    16081 ttcaaaaaat aaagaaaaat cagtagaact tctagggctg cttaacagtg atccagaatt
    16141 attgaatggg cttgtttggg ggatcgaggg agaagcatgg gaaaaagtcg atggttcaga
    16201 tgataaaatc aaattgctag atggttatca gccaaataca catatgtctg cttggaatac
    16261 aggaaacaac aagattctat atacacaaga gtctattacg gatgacatga ttgccaaacg
    16321 tgatcaatcg attgctgatg cagaaacttc accgatttta ggattcagct tcaacactga
    16381 cagtgtcaaa acagaattaa gtaatatttc taatgtcatg aatcaatatt tagatggatt
    16441 gaatacaggg actgttgatc cagatgaaac attaccgaaa ttgaaagacg ctttggacaa
    16501 ggctggctat gataaggtat tgaaagaaat gcaaaaacaa tatgatgagt tccgacaaga
    16561 ataataatga ataaagacaa aggggttgtg acaaaagacg tgtcacaacc tcttgttccg
    16621 aataaacggt gttcaaaagc cagtttctcg gtattaagct aaatatatgt aaaaatgtgg
    16681 aaagccatat tcacaaaatt tttcttacta ctcgaaacta agcggctatt tcacaaactc
    16741 ttggtaaacg gtgttcaatc agctgaccct cggtattcgc tcacaaaacg acaaaatatg
    16801 aggaactatt tttccgtttg tttctctaat aatcaggtca aggtgctgat tgcccaacct
    16861 ttttttgtat aatgagaaag tataaactta atgtttggag gagaagatat gttagggaag
    16921 gttttggaag agttatttat ccggatctgg gtcattataa aactaacctt gtatttctgg
    16981 atatatacat ttgccggcgg gatcatcttt ggattaggtg cggcatggaa aacagtcaat
    17041 gaactatttt atttgtacgg attcgagtat aaagaaatca caataaaaag aggctggaat
    17101 atatataaaa gaaatttttt aagaggcaat ctgctattta gtttattttt atctggtact
    17161 gctttattga gttacaatt
//
LOCUS       DACDUU010000064        16866 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.064, whole genome shotgun sequence.
ACCESSION   DACDUU010000064 DACDUU010000000
VERSION     DACDUU010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.064"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            99..416
                     /locus_tag="IWL30_002144"
     CDS             99..416
                     /locus_tag="IWL30_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706877.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNASIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(619..984)
                     /locus_tag="IWL30_002145"
     CDS             complement(619..984)
                     /locus_tag="IWL30_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706878.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1192..1674
                     /locus_tag="IWL30_002146"
     CDS             1192..1674
                     /locus_tag="IWL30_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706879.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1871..2761
                     /gene="pstS"
                     /locus_tag="IWL30_002147"
     CDS             1871..2761
                     /gene="pstS"
                     /locus_tag="IWL30_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAP9706880.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWEIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            3086..5560
                     /locus_tag="IWL30_002148"
     CDS             3086..5560
                     /locus_tag="IWL30_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9706881.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLLKRRNNTVAMTGDGVNDI
                     LAMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLV
                     KNIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNV
                     MEKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKF
                     RSQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLF
                     ERKNEKEQKPFSRVVKKFLSIFKREN"
     gene            complement(5663..7399)
                     /locus_tag="IWL30_002149"
     CDS             complement(5663..7399)
                     /locus_tag="IWL30_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAP9706882.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNGDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRAYTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLHGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(7396..8100)
                     /locus_tag="IWL30_002150"
     CDS             complement(7396..8100)
                     /locus_tag="IWL30_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9706883.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            8231..8734
                     /locus_tag="IWL30_002151"
     CDS             8231..8734
                     /locus_tag="IWL30_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAP9706884.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            8694..9032
                     /locus_tag="IWL30_002152"
     CDS             8694..9032
                     /locus_tag="IWL30_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAP9706885.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            9053..10471
                     /gene="ffh"
                     /locus_tag="IWL30_002153"
     CDS             9053..10471
                     /gene="ffh"
                     /locus_tag="IWL30_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAP9706886.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            10583..11161
                     /locus_tag="IWL30_002154"
     CDS             10583..11161
                     /locus_tag="IWL30_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAP9706887.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            11165..11686
                     /locus_tag="IWL30_002155"
     CDS             11165..11686
                     /locus_tag="IWL30_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9706888.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(11765..12319)
                     /locus_tag="IWL30_002156"
     CDS             complement(11765..12319)
                     /locus_tag="IWL30_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP9706889.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            12572..12847
                     /gene="rpsP"
                     /locus_tag="IWL30_002157"
     CDS             12572..12847
                     /gene="rpsP"
                     /locus_tag="IWL30_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAP9706890.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            12859..13110
                     /locus_tag="IWL30_002158"
     CDS             12859..13110
                     /locus_tag="IWL30_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP9706891.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            13235..14026
                     /locus_tag="IWL30_002159"
     CDS             13235..14026
                     /locus_tag="IWL30_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706892.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            14196..14717
                     /gene="rimM"
                     /locus_tag="IWL30_002160"
     CDS             14196..14717
                     /gene="rimM"
                     /locus_tag="IWL30_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAP9706893.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            14707..15468
                     /gene="trmD"
                     /locus_tag="IWL30_002161"
     CDS             14707..15468
                     /gene="trmD"
                     /locus_tag="IWL30_002161"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAP9706894.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            15604..15951
                     /gene="rplS"
                     /locus_tag="IWL30_002162"
     CDS             15604..15951
                     /gene="rplS"
                     /locus_tag="IWL30_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAP9706895.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(16083..16844)
                     /locus_tag="IWL30_002163"
     CDS             complement(16083..16844)
                     /locus_tag="IWL30_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9706896.1"
                     /translation="MIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKNPIDEVVIDY
                     KRESKTIKIKDKFLEDDEYNRLVDFTTSHNKRYSLLFQWLYLTGMRSGEAIALSKDDV
                     HITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAIYNELLELNP
                     NGQFLFQTTKGTPFQLTAINTYLRSHKADMKIDKKLSSHIFRHTHISKLAELGTPLYA
                     IQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
BASE COUNT         5607 a         2979 c         3393 g         4887 t
ORIGIN      
        1 atatcttttt acgaaaaaca tcacaaggaa aaacttaact atggtattat ctaaacataa
       61 ttttttcggt atttaatatt taattaggag gtaatatttt gcttttttat agtaatttta
      121 tcttaatagt agcgatatta ttacttctga atatatggat ttttgatagg agtagaaatg
      181 caagtatagg ttttagaact aaacgaagtc tgtcttctaa aaaaaattgg gtatactcac
      241 aaactatttt ttatggagga attgttttaa ttagtttact ttcttcaaca ctttattctt
      301 tgaatattat agatgtttcc acctcaaatt caatatctat tattggaatc attattgctg
      361 caattattac acaactgttt ttagtgtttg gagagaagaa aagaagtaaa aaatagaaaa
      421 actagtgata gttttggtga tagtgatatt aaaaatcaga aaagatagaa aaagcaggag
      481 atagataaaa tgctgatatt tcagcatttt taagagtata aaagagaaaa aaagtaataa
      541 atgtatacat gatataagaa aaacatatat gtataaaaag aagaaagtag cttctctttg
      601 tacatatatg gtttgttttt atggatgccg agctttttta taagataagg ctaccgccca
      661 taacgaccca aggatttgtg gaactaagta aatacctgca agaagcaaga tcagctgggt
      721 cggttttaaa ggatgataga agtaacgcca ttcagtaaaa ttggcgcaaa ataaatagga
      781 agtgacagta gatataatat tggcaatgaa taaagatata atacgcctgt tcctttgaaa
      841 ataatatcca gcaaaaagag ataaaaatat caatgtgatg aacccgctta cggaatgata
      901 acgaaaatca agcagtaagg cgaaatgcac ataaggaaca aaagataaaa gcaacggaag
      961 gtttttccag caagttttta tcatctttca tcaccaccta acaagtgtta ttattttgtt
     1021 agttttattg taaattgaat cctgcaataa tcaattactt tttcattatt tctagattaa
     1081 atgaataaac tctcatgcca ttatctctaa aaaaaaagtt tttcccccta atttttttag
     1141 atagaaaatt gtatagtatg atgagagaaa aagtagtttg ggggaacgtt aatgagaaga
     1201 aaagcagtaa tcgatcggct ttatttattt ttttggtttt ttcctatcgt tttgctacct
     1261 tttacatggg taaatgctgt tgtacaaggt gcggagatat taggcctgat ccaaggaagc
     1321 ggatttgtgg tttctaggaa ttttccagct ttgactgttt tctatttagt gataagtgta
     1381 ggtctcttat taccaaaatc tctgatcaat cactacaatt ggcagctgct ttacttttct
     1441 tgcttattag tatttacatc tatgttttct ttgctgcctc gcttgattat cggccctaat
     1501 ggtttgagcg agagtggatt gaaggattca gcttattcct ccatgtattt cttgacgatc
     1561 aaaccatggt tctatttagt aattgcctca tttattcttg caacattttt ttacttctgg
     1621 acagaaatca ggataaaaag tgaacgatct gtatctataa aaaataacta ttgatggtgg
     1681 atcatcagta aaaaagcata tgagctgcgt aaaatgcatg tttctcatat gcttttttta
     1741 ttattatgta aagaatgcga aaaacgttta catgattttt acataactta actatatttt
     1801 tacagcaacc atacgaggga tttacagtcg actgataaac tttagttgta aataagaaag
     1861 gagcaataaa atgaaaaaag tattgtttac tctaggtatg atgggaattc ttttagccgg
     1921 atgcggctca ggatcaggaa atacagatgg ctctgccaca caaaataatt caagtgactc
     1981 aaatgaacaa gtaaaaatcg ttgcggtagg ttcgacagca ttacagccgt tagtcgatgc
     2041 agcacaagaa agctttgtac aagaaaatcc aaattatcaa atctctgtac aaggaggagg
     2101 aagcggaact ggtttgagcc aagtcgaagc tggagcagtt actatcggaa actctgatgt
     2161 atttgcagaa gaaagagacg gagttgatgc ttcaaaatta gtggatcata aagtagcagt
     2221 ggtcgggatg gcaccaatcg tcaataaaga tacagatgtc aaagatatca ccaaacagga
     2281 attgattgat atctttacag gaaaaatcac taactggaag gaagttggcg ggaaagatca
     2341 aaaaatcaac gtcgtgaacc gtgcaaacgg cagcggtacc cgcgcgacat tcgaaaaatg
     2401 gggactggat ggtgctaccc ctgtgcagtc ccaagaacaa gattcatcag gaacagttcg
     2461 gcagctggta agtcaaacac caggagcaat cagctattta gctttctcat atctcgatga
     2521 ttccacacaa gcactaagta tcgacggtgt agaaccaaaa gaagagaatg tagcagataa
     2581 cagctgggaa atctggtcat atgaacatat gtacacgaac ggaaaacctt cccctgaagt
     2641 ccaaaaattc ttagactaca tgatgacaga agaaatccaa gaaggacctg tcaaagaatt
     2701 gggctattta ccaatcacaa tgatggaagt agaacgtgat cacgaaggaa atatcaaata
     2761 actagcagat aaacatgcca cggtagtcgt tactattaag gaaaaatcaa ctatgtttcc
     2821 ccacatatac atttttcctt taattgtgaa aaatagttcc tcatattttg ccgttttttg
     2881 agctaatacc gagggtcagc tgattgaaca ccgtttattc gaaaaaaaga gctacgacat
     2941 agaactgtcg tagctttttt ttgctatatg acaggaatga aaggtgtttg caagcttaat
     3001 gtgaggaaca ctttccatat ctgttctata ctggatagga gaagtttgga aatatccaga
     3061 aaacgaaatc aatcgaggta gccacatgaa gaataaatgg ataaatcgaa gtaaaaaaaa
     3121 gaaagtaaca caaagatcat cagataaagt catccgtctt tttccatcgt ataaagaagg
     3181 attaacggca gatcaagtca aggaacgaat agaaaaaggt gcagcaaata attcagtcga
     3241 tcccacattc aaaacaaatc agcaaatcgt attggaaaac atatttacgt atttcaattt
     3301 gatctttctt atcttagcca ttttgctttg tttagtagaa tcctataaga acttgacttt
     3361 tttaccagtt atcatagcca atacaggaat cgccattttt caggaaattc gatccaaaaa
     3421 gatcttggac gaactaaatg ttttaaatac agcaaaagtc aatgtcatac gagacgggat
     3481 agaacaaaca atagatatag aagaccttgt aatagatgac attgttattt tagagactgg
     3541 acatcaaatt ccagcagatg cagaggtcgt ggaagggaaa ctgcaagtta atgaggcttt
     3601 attgacaggc gaagcagatg aaataaccaa agaaataggc gatccattaa tgtccggcag
     3661 tttcattgtt tccggaaaag cttacgcccg attagacaaa gttggagcag attcgtatat
     3721 ttcccagctg acagtcaaag ccaagacaat gggagaagga gaacagtcgg agatgattgc
     3781 ttctcttaat caattgatca aatggattgg gatcatcatt attcctatcg gtatcctgct
     3841 tttttctcaa agttactttt ttaatggaaa cacgatcaaa gaaagtgtcg ttgcaatgga
     3901 agcagcattg atcggtatga tacctgaagg actgtacttg ctgacgacga ttgcattagc
     3961 attaagtgcg actaaactag caaagcaaag agttctttta cacaatatga aaagcattga
     4021 gacattggca agggtaaatg tcctttgtgt agataaaaca ggaacgatca cagaaaataa
     4081 aatgtctgtt caaaaagtga tcgtctctaa aaaacaagaa ataatcagtc agtcagatca
     4141 attggaagca tgcattgctg actatgcaaa agcgatggca ggagacaatg caacaatgga
     4201 agctgttaaa gcatatttta caaaaaccac tgatcaatcc tatcaccatg ttattccttt
     4261 ttcttctgtc caaaaattca gcagtgtagc gttggcagac aaggtgtatg ttttaggagc
     4321 gcccgaaatg gttttgcgcg atcaattgcc ggaatatgcg gaagagtttg tttctttcgc
     4381 tgaaaaaggc tatcgcgtgt tggtctttgg tgtgtacgat gggattcttg aagaaccagt
     4441 attgacagaa gcagtcacac cattaggcta tattctgatt gctaatccta tcagaaaaga
     4501 agcaagagcc acatttgatt acttcaaaaa acagcaagta gccatcaaag tgatttctgg
     4561 agataatccg ctcaccgtat ccaatgtagc tgtccaagca gggatttcac atgctgatca
     4621 gtacgttgat gcatctaaat tatcagaaga agaatatgaa gcagctgtcg aaaaatatac
     4681 agtttttgga cgcgtcaagc cagaacaaaa gaaaatcttc gttcaattgc taaagcgtcg
     4741 taacaataca gtagctatga caggtgatgg cgtgaatgat attctagcca tgaaagaagc
     4801 agactgcagt atagcgatgg cttccggaaa tgaagccgcc atgcaggcct cacaagtggt
     4861 cttacttgat tctgactttt cacgcatgcc agaagttgtt gcagaaggac ggcgcgttgt
     4921 gaacaatatt gagcgatcag ccagcttgtt tttagtgaag aatattttct cctttttgct
     4981 ctcactgttt tcagtgatct ttgctctgac ctatccttta gaaccatcac aaatcacatt
     5041 gattagtttg tttactatcg gacttccatc tttcttgctt gctctagaag aaaacaaaaa
     5101 gcgaatcaga gggaaattca tcatgaatgt gatggaaaaa gctgtgccag gtggactaac
     5161 agatatgatc gtagtcggtg cgttggtgat ctgcggggtg actttgaatc taaataaaac
     5221 agatgtttct acagcatcta ccatgctttt gattgcagta gggttcctcg ttctctataa
     5281 aatctgttca ccgctgaata aattcagaag tcagatcatt cttttctgtg ctagcggtaa
     5341 ctttttttcg gtcatctttt tgcataagct gttttcaatc accggtatct cagctgtatc
     5401 acttttgctg ttgagcattc tatttttctc ggcagattcg atttttaggc atttgaccac
     5461 attggtagaa tatttgtttg aacgtaaaaa tgaaaaagag caaaagccgt ttagtcgcgt
     5521 agttaaaaaa tttctatcga tatttaaacg agaaaattga cgaaaagtat ctcaaaaaag
     5581 agtaaaaaag aacaaccact cgtgtatctt tcgaagcagg atgagtcatc caactttaaa
     5641 acgcacgagc agttgctctt ttttacattt tcggaatggt aacggtgaaa gtcgaacctg
     5701 ttccaagatg actgtctaca gtaatcgtac catgaaggag ttgcacgtag tcttttacaa
     5761 tcgctaaccc taatccggtc ccgcctgaat gccggcttcg cgccttgtct acgcggtaaa
     5821 aacgctcaaa gatccgttcc tgatcctcaa tgtcgatacc tataccaaag tcttggacag
     5881 aaaacgatag atcatctgtt gcctgatagc ggataataat ctcgctgtcg ctttttgaat
     5941 attggacagc attttcaatc agatttttga taattggata aaggatatct gttttagtag
     6001 taaatggttc gttctttcca atcaatcgaa ttgtcaaccg tttcttttcg atgattggct
     6061 gataattttg aacgatttgc tggaaataag gttctgtata aattgtctgg atctcgtaag
     6121 aattctcgcc atcttttgac agctggatga tttcccgaat cagtttatct aaccggatcg
     6181 catctttttg catgatctca agaaaagaag tcagagtttg cgggtcatct tttgcaccat
     6241 ccagtaaagt ctcggtaaaa ccaatcagtg aagtcacagg agtttttaat tcgtgagaga
     6301 cgttgccaac gaaatccttt tgtagctttt ctaaacgtcg cactttcgtt aagtcatacg
     6361 ccacccctag aatctgtccg gtaccttctg aattagtaaa gtagcgcaaa ctcaagtcta
     6421 atgtctgccg accttctgta agggtgatct cctgatggat cagcggatgc tcaggagtca
     6481 cttgatgaat cagctgaatg agttttggct cttgaatgac ttctgtgtac cgctgttcag
     6541 gttgatagct taccacgttt aaatgtagct gcatctttgg attaagcagt agtaaacgag
     6601 aatcagcatc atcaatcagg aatacgccaa tcatcagttc gttcagtaac gtatataatt
     6661 gctcttctgt ggtagtatac gcccgataca tcttactcat ttgttcgctc aacgcattga
     6721 tcgtctgata caattcttcc cattgatcgg gtgtttgcat gattacttcc gcctgattag
     6781 gattttgcac catttttttt agtacgggca gcacggtttt caatggctgg tttttttgat
     6841 agatcagata atagatcatt cctgcaagaa caaggaagaa agacagaaag aagaaaaaga
     6901 cccaacggcg aaaactctca gttctcggta aaaaaccgct tgtcggttct gcgatacgta
     6961 aaatggcttc taactggcca ctttttttga ctggtaatgc gacatataaa agatccccat
     7021 tcaaagtagt acttttgcgc aaagcgctac ctaggttccc accattcaat acggctttga
     7081 tttcgggacg ggagtttcgc tgttcattca atgtttcatt gtttgtgtca aataagatct
     7141 ttccagtagc atccattaaa gtaatccgtt cgttcgactg atggacgaat tcttccaaag
     7201 cagttttgtt ttgcgctgct tggaggtttt cgacatctaa ttgccggatc agcagttgtg
     7261 ctttttttgt caggtagctt tcttgctgtt ccacgatctg ctgttgaaaa taatgggaaa
     7321 tcaattgcca gccaccaaaa aacaaactaa ctaaaagaat aagaaagaag agacgttcat
     7381 aaattttttt ccatttcatc gtttgggttc ctgaaactta tagccaaatc ctctaatagt
     7441 caccaagtac tttgggtgtt ttggatctcg ctcgattttt tctcgcaaat gactaacgtg
     7501 tacatctacg attcggcttt gtcctgcgaa gtcaaaattc caaatgcggt ctagcaatgt
     7561 gtctcggtcg atcacacggt cttttcgttt catgaaataa acaagtaatt caaattcttt
     7621 cggtgttagt tcaatcagat ggtcgtctac ggtcacttga taattggtca aatcagcagt
     7681 gatttgtcca atcgatagat attcaggctc agcttcatct tttgattgct tacgaggttc
     7741 aattcgtctg aatattgctt tcatacgggc taatacttcg cgaggactaa atggtttggt
     7801 caaatagtcg tcagcaccta tttctaggcc aatgatccga tccacttggt catctttagc
     7861 cgtcagcatc aaaatcggtg tttcattttt ttcttgacgc agccgctgag tgatctccat
     7921 gccatccatc gttggaagca tcacatctaa aatgatgaaa tcaaatgatt gttctaatgc
     7981 taattctaat ccttcgcctc catctgtggc actggttacc ttgtagcctt ctttttccaa
     8041 attgaacgtc aagagcgtta caatagaagg ttcatcatca acaactagta tttttttcat
     8101 tcaaatctcc tttaaatagg tttcattctg tttatatttt agcatagaat cagtacaaag
     8161 ggctatttta caaaagaaat aaatggtata ataggaaggc actttgctga atagaaagga
     8221 agagcagtca atgattggaa tcagtgcatg tttaggcggt ctggcctgtc gctatgatgg
     8281 acagaaaaaa gaagtaccag aattgatgga actagttgaa actggagaag ctgtcatggt
     8341 ctgtccagaa gtgatcggcg gattaccaat tcctagagat cccgctgaaa tcattggtgg
     8401 aaatggattc gatgtgtgga aagatcaagc aaaagtatgg acgacaagcg gcgaagatgt
     8461 aacggaagcg tacaaaaatg gcgcgatcaa agcctatcaa aaattgcagg aaaaacacat
     8521 tgatctagtc atcatgaagg aaaaaagccc ttcatgcgga acccattcga tttatgacgg
     8581 aagtttttcc ggaataaaaa aaaatggtgc cggagtggct actgcttatt ttattcagca
     8641 aaaaatgact gtgctctctg atgaagaatg gctgaaaact agaggtgagc agaatggaaa
     8701 tcgaaaagac caatcgaatg aacgcgctct ttgaatttta ttcgacctta ttgacagaaa
     8761 aacaaatgaa ctacatggaa ttatattatg cagatgattt ttcattaggt gagatcgcgg
     8821 aagaatacga agtaagccga caagcagtat atgacaatat caagcggaca agcaagatct
     8881 tggaggatta tgaaaaaaaa ctccatcttt tttctaatta tattgttcga gaacaggtat
     8941 tggaaaatat gactgcatat attacagata agtacccgga agataaaaaa ctacttgaat
     9001 atgtacagca aattcaagca atcgaagaat aaataaaagg aatgaagaaa atatggcatt
     9061 tgaaagctta actgaacgtc tccagcaggc gatgggaaaa ataagaaaaa aaggaaaagt
     9121 atcagaagct gacgtaaaag aaatgatgcg ggaaatccgc cttgccttac tagaagccga
     9181 tgttaacttg caagtagtca aagattttac taagcgcgta cgcgaacgtg cggtaggggc
     9241 agaagtgctg gaaagtttat cacctgcaca acaaatcgta aaaatcgttg atgaagaatt
     9301 gacgatcaca cttggttctg aaacagcaga gttaaataaa tcaccaaaaa taccaactgt
     9361 tatcatgatg gctggtcttc aaggggcagg taaaacgacc tttactggta aattagccaa
     9421 ttatttaaag aaaaatgaaa atgctcgtcc gctcctaatc gcaggggacg tatatcgacc
     9481 agctgcgatc gaccaattga aagtattagg tcaacagcta gacgttcctg tatttgatat
     9541 gggaactgaa gtcagcccgg tagagatcgt ccgtcaaggg atggagttag caaaagaaaa
     9601 gaaaaacgac tatgtattga tcgatacggc aggacgtctg catatcgatg aaacattgat
     9661 ggatgagttg aaacaaatca aagaattgac gcaaccaaat gagattttgc ttgttgtcga
     9721 tgccatgact ggtcaagatg ccgtcaacgt agctgatagc ttcaaccagc aattaggtat
     9781 tacaggggtc gtcatcacta aacttgatgg ggatactcga ggcggggcag cactatcgat
     9841 ccgctcagtg acaggcgcac caatcaaatt catcggttct ggtgaaaaat tgactgactt
     9901 agaagtattc cacccagatc gtatggctag ccgtatcctt ggtatggggg atatgctgac
     9961 attgattgaa aaagcgcagc aagactacga cgagaaaaaa gcggaagaac ttgcacagaa
    10021 aatgcgggaa aattcattcg atttcaatga ctttattgaa cagcttgatc aagtgatggg
    10081 tatgggccca ctggaagatc taatcaaaat gatcccagga atgaatcaag tccctggaat
    10141 cgaaaacgtc aaggtagatc caaaagatgt tgaacgcaaa aaagcgatgg tttattctat
    10201 gacgccagca gaaagagcaa atcctgatct gttgaaccca agccgtcgtc gccgaattgc
    10261 tgccggttct ggaaacagtg ttgttgaagt gaatcgaatg atcaaacaat tcaaggaatc
    10321 aagaaaaatg atgcagcaaa tgtccaaagg ggatatgaac atcccaggta tggaccaaat
    10381 gtttggttca ggcgtcaaag gaaaactagg caaaatggcg atgaatcgaa tgatcaagaa
    10441 aaataagaag aaaaagaaaa aacggaaata agatgaaaaa aacagcttcg gtacagactg
    10501 aaaatgtcta ctgccggagc ttttctatca gcaattttta ttctttacta atacaattac
    10561 tctaaacgaa gaggtggcac agatgagatg tccgtggagt gaacaaacag aaagtatgcg
    10621 gcaatatcat gatactgtct ggggtgttcc tgaatatgat gaccaaaagc tgttccgaaa
    10681 attgatgctt gatattaatc aagccggatt gagctggcag acgattttaa ataaaagtga
    10741 agcattcgat gaagcatatg atcgattcga tatcaaaaaa gtcgcaaatt atggagaaac
    10801 aaaaatcagt gaactaatgg aaaacaaagg aattattcgt aaccgaagaa aaatcgaagc
    10861 agccgtccaa aacgccaaag cagtcttgaa aattcaagaa gaaatcggca gtttttcaga
    10921 gtatctttgg tcgttttcta atggaaaaat cgttgattct tcttatcatt cacaagaaga
    10981 agtcccaaca acaagtcctc tttcagatga attagcaaaa gatctaaaga aaagaggatt
    11041 taaatttatc ggaagcacaa caatctatgc ttttttagaa gctgtcggga tcatcaatga
    11101 tcaccttgac agttgtttcc gcaaagaaga ggtcgtacgt atcggcagaa agggcgaata
    11161 aagcatgcga aaaatcatat tttatggtgc tataagttta gatggctatt tggcaacgaa
    11221 aacggatgat cttcaatggc tttttgacac tccaacaggt aagcaaacta cttatgaagc
    11281 tttttatgaa acaatcgata cgaccatcat gggcagaaaa acttatcagg aagcaaaaaa
    11341 atatttggag accgaagcaa tttatccaga gaaaaagaat ttcgtttttt ccacccacgc
    11401 acaattacaa ttgccagatg ctgaggtcgt aaacgaagat cctgttgctt ttctaaaaaa
    11461 tctacaaatg acagaaggaa aagatatctg ggttgtgggt ggcggtcgat tgatcaaacc
    11521 gctattggaa catcaaatga ttgatgaatg gtatatccag attacccctg tattattagg
    11581 ggacgggatt cctttattcc aaaaaggaac atatgaagcg cgttttgaaa tgctagacac
    11641 gacacgattc ggtgaattta tcgaattgca ttatgtacga aagtagaagt aaataaaata
    11701 aaaaaggcaa ctatatgcag aaatgtcctg tacatagttg ccttttttat taccgttatg
    11761 ctttttactc tgttaatttg ttagaaaaat ctagatacgc atctaaaatc gtttggaaga
    11821 aacttaatgt tccttcaacg atattaccat tttcatcaat tagattcgca acgtttccga
    11881 tatatgcttc cggttgctgc aaggttggca cattcaaaaa gacaagtgat tggcgtagat
    11941 ggtggtttgc gccaaagccg ccgatcccac ctggtgatgc agtaacgact aaacctggtt
    12001 tatgatccca aacactgtgt ccataaggtc ttgagcctac atctaaagca tttttcaata
    12061 ctgctggcac agaacggttg tattcaggag taacgaaaac gacaccgtct agtcccctca
    12121 cttcctcacg aaaacgagtc cattcagcag gagcctgttc tggagtttcg agatcctcat
    12181 tgtaaaatgg cagatcatcg atttttacaa atacactttc gtacccttca ggaagcaatt
    12241 tcgcaaaagc ttctgctact tttttgttgt aagaatcttt tcttaaacta ccaataaaaa
    12301 atccaatttt ttttgtcata cgatttttcc tccttcaaac tatctataat aatcttacaa
    12361 aagataagta actggagcaa ataaaaacac ttactgacaa aagctacata gaagatagat
    12421 tacttatttt taacaaaaaa gaagtctaaa atcttctatt ttctaaaaaa aatcgaattt
    12481 aatcaagctg taaagaaaaa aagctttaca gggtaaaaaa aaactgttac actacatctt
    12541 gtgaataaaa caatggaggt ggaaataaat aatggcagtt aaaatccgtt taaaacgtat
    12601 gggttctaaa aagagtccgt tttaccgtat cgtcgtagct gattcacgtt ctcctcgtga
    12661 tggacgtttc atcgaaactg taggtactta caatcctttg aaagaccctg cagaagtagt
    12721 tttaaaagaa gacttagttc ttgactggtt atcaaaaggt gcacaacctt ctgacactgt
    12781 acgtaacatc ctttcaaaag aaggcgttat gcaaaaacac cacgaagcta aattctcaaa
    12841 gaaataaggt gaccggatat gaaagattta agcgacttag tcttaacaat cgttcgtccg
    12901 ctagtcactt atcctgatca agtcaagtta gacgtcgtag agtcgaaaga cttttacgag
    12961 tacaatctaa cagtggctcc tgaagatatt ggccgtatca ttggaaaaca agggcgtgtt
    13021 gctaaagcaa tccgcacaat cgtttacggt gttcggatca atgcaccgaa aaaagtgcgt
    13081 ttgaatatca tcgatgataa ggaaaagtaa ataaacgtgc tgtactcgaa ttttcgagtg
    13141 cagctttttt gtttttttta ttaattgaag aaatgattat ttggcaaaac aagcaagaaa
    13201 gcgtatcctt ataggaggag ggagtgaaca gccaatgaga aagaaaatgt tatttattcc
    13261 atttgctcta ttgttaagcg cttgtagttc ggttactcaa gaatcagaca atacacatct
    13321 atcaagcagt atatctgaaa ctgttaccag tccagaaaaa agtagtacca aaacggcttc
    13381 caccactgaa caaacaatgg ctacctcaaa taatgagaaa aagactgctt tagaccagtt
    13441 gaaagaacaa cagccaaatg ttcccatgcc tctagatgta ccagtctctt caggctatct
    13501 caatatcgca gcgacacata caaagcaagg ttattctatt ttatattaca gaacagatcg
    13561 tcccctcggg ttgaatgctg acgaattgaa tcaagaaacg cctattgcaa cctatctata
    13621 tcaatatggg tttgcttcta gtcaagaaac aatccaagtt ttgcagccat ttgagatcga
    13681 cacaaatggt cagcaagtgg acttaggttc tcgaatcaca gggtatcagc aaggtgcagc
    13741 aggttcaagt tttcttgaat ggcaagaagg aaactggcgt attcgaatcc gtgggaataa
    13801 tatagaaggc caagatccct tactattagc taaagaaatc gtagcttatt tggaagaaaa
    13861 cagtctacca gcacctgaac aatttggtaa aattactgtt gatatgggtg atacgacgaa
    13921 tcgagccgta gaagtaagct ggcaagaacc gaaaaatgcc tatacgatca ctcatcaaga
    13981 tcctatgtct gcgttgaaga tggcagtttc tatgaagagg ttgtgaaatc agcgcgctga
    14041 tctgagccca tcatttaact ccaagtcaca ggaagtaatc aaaaatagca cgccgcattt
    14101 ttgcataatt ttggcttaat accttaaaag aaaactagaa ttgcctcttc acacatgcta
    14161 aaataaaaca gacaaaaaag aaatggggat ataagttgac tgaatacttg aatgtaggaa
    14221 aaatcgtaaa cacacaaggt atcaaaggcg aagtccgtgt tatctcaaca acggatttcc
    14281 cagaagaacg ttacaaaaaa ggaacggtgc taacactatt ccaagacgga aaagcacctg
    14341 tggaattaac agtcaaaagt catcgcaaac ataaaaattt tgacttattg agttttgaag
    14401 gacatccttc tatcaatgat gtggaaaaat atcgggatgg catattaaaa gtgtcaaaag
    14461 acaactctgt agatctagct gaaaatgaat tctattacca tgagatcatc ggtctgcatg
    14521 tattagaaga tggaactgag ttaggtaaag taaaagaaat cttgtcacca ggtgccaatg
    14581 atgtttgggt cgttcaacgt ccgaaaaaag ctgatatatt gcttccatat atcgcttctg
    14641 tcgtcaaaga agtcgattta gaagctggag tggtccgtgt agaaattcca gaaggactga
    14701 ttgacgatga agattgatgt attgacttta tttccaagaa tgtttgaagg tcctatgggg
    14761 gaatccatca tcggtaaggc tgtagataaa ggattattag atatcaatat ctctaacttc
    14821 cgcgattatt cagataacaa acatcagaca gtagatgatt atccatacgg cggtggagca
    14881 ggtatgctgc tcaaagtcca gcccatctat gataacatcc aagccattga aaaagctgca
    14941 ccggatgtca aaaaaagagt aattcttctt gacccagctg gtaaacgatt cgatcagaaa
    15001 atggcagaag aattttcaca agaagaacac ttagtattca tttgtggtca ttatgaggga
    15061 tatgatgaac gtatccgcac attagtgaca gatgaagttt ccttaggaga ttatgtcctt
    15121 actgggggag aattaggtgc gatggtcatg atcgatgcaa cagttcggct tttgccagat
    15181 gtgttaggga atcagacttc agctcaaacg gattcttatt caaccggttt attggaacat
    15241 ccccaatata ctcgcccggc agaatataaa gggatgaaag taccagaagt attgacaaat
    15301 ggaaatcata aattgattga agaatggcaa ttaaaagaat cactaagaag gacctatcaa
    15361 cgtcggcctg atcttttgga aacacttgaa tggacacctc aaatgacgaa atttttagaa
    15421 gaaatcaaga aagaagaaca agaaaaaaca gcagatgaag ccaagtgact ttttctaaaa
    15481 tcttctttac agtaggttga taatatgtta gtattttatg gtgagtgcaa aagcacttaa
    15541 ctattacgat attccgctgt gagaaaagct cataagaata tttggaataa ggagaagaaa
    15601 accatgaatc cattaatcga agaattaaca aaagaacaat tacgttctga catcccagcg
    15661 ttccgccctg gtgacactgt gcgtgttcac gcaaaagttg ttgaaggtac tcgtgaacgt
    15721 atccagttat ttgaaggtgt tgttatcaaa cgccgcggtg ctggaatcag cgaaacttat
    15781 acagtacgta aagtttctaa cggagtgggc gttgaacgta cattcccatt gcatacacca
    15841 cgtgttgcaa aaatcgaagt agttcgctac ggtaaagtac gtcgtgcaaa attgtactac
    15901 ctacgtgcat tacacggaaa agctgctcgt atcaaagaaa tccgtcgtta atcatcgtta
    15961 tcagacagag aaagaaccct tggtattcaa gggttctttt tttattatct gtttttttat
    16021 attaacgatt tctatttggt acatttttga ttaataaaaa ggggcaaaaa aggggcaaca
    16081 agttacagtt tttctatatc ttctttcaat ttttctttta ctccttttgt cacatgcaaa
    16141 taaatttttt cagtgatatc actattttcg tgaccaacgc gatcctgaat ggcatacaga
    16201 ggagttccta gttccgctaa ttttgaaata tgggtatgtc taaaaatatg tgaactaagt
    16261 tttttatcaa tcttcatgtc agctttatga cttctcaaat aggtgttaat tgctgttagt
    16321 tggaaaggag ttcctttcgt tgtttgaaat agaaattgac catttggatt taattctaga
    16381 agctcattgt aaatagctat cgcttttttt ggtaaatcaa tttctcgcat tccggcagct
    16441 gtttttgtag aatcagattt tttcatatca gctattgaac gttctctata catcatcgtc
    16501 ccatttataa ctactgatgc attattattg gtaatgtgta catcatcttt acttaatgcg
    16561 attgcttcac cagacctcat cccagtcaaa tatagccact ggaaaaggag agaatatctt
    16621 ttattgtgtg aagttgtgaa atctactagt ctattatatt catcatcttc taaaaattta
    16681 tctttaattt ttatcgtttt tgactctctt ttataatcaa taactacttc atcgatagga
    16741 tttttttcta cataaccttt tttcatagca tatgaaaaaa gaagattcaa tttggattta
    16801 attacactga cgtacttatt tgacaagtca tctttatata tcatgtcttc aaaaatatta
    16861 ttcaaa
//
LOCUS       DACDUU010000065        16278 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.065, whole genome shotgun sequence.
ACCESSION   DACDUU010000065 DACDUU010000000
VERSION     DACDUU010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.065"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..2387)
                     /gene="esp"
                     /locus_tag="IWL30_002164"
     CDS             complement(<1..2387)
                     /gene="esp"
                     /locus_tag="IWL30_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAP9706897.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVS"
     gene            complement(3005..3439)
                     /locus_tag="IWL30_002165"
     CDS             complement(3005..3439)
                     /locus_tag="IWL30_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706898.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3456..4661)
                     /locus_tag="IWL30_002166"
     CDS             complement(3456..4661)
                     /locus_tag="IWL30_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAP9706899.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            5355..5741
                     /locus_tag="IWL30_002167"
     CDS             5355..5741
                     /locus_tag="IWL30_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9706900.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            5835..6026
                     /locus_tag="IWL30_002168"
     CDS             5835..6026
                     /locus_tag="IWL30_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9706901.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            6540..6872
                     /locus_tag="IWL30_002169"
     CDS             6540..6872
                     /locus_tag="IWL30_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9706902.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            7168..7323
                     /locus_tag="IWL30_002170"
     CDS             7168..7323
                     /locus_tag="IWL30_002170"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP9706903.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            7599..8939
                     /locus_tag="IWL30_002171"
                     /pseudo
     CDS             7599..8939
                     /locus_tag="IWL30_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9180..10796)
                     /locus_tag="IWL30_002172"
     CDS             complement(9180..10796)
                     /locus_tag="IWL30_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP9706904.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            10914..11132
                     /locus_tag="IWL30_002173"
     CDS             10914..11132
                     /locus_tag="IWL30_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9706905.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            11327..11788
                     /locus_tag="IWL30_002174"
     CDS             11327..11788
                     /locus_tag="IWL30_002174"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9706906.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(11981..12220)
                     /locus_tag="IWL30_002175"
     CDS             complement(11981..12220)
                     /locus_tag="IWL30_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706907.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(12244..13767)
                     /locus_tag="IWL30_002176"
     CDS             complement(12244..13767)
                     /locus_tag="IWL30_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP9706908.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(13785..13907)
                     /locus_tag="IWL30_002177"
     CDS             complement(13785..13907)
                     /locus_tag="IWL30_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP9706909.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(13937..14920)
                     /locus_tag="IWL30_002178"
     CDS             complement(13937..14920)
                     /locus_tag="IWL30_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP9706910.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(14944..15093)
                     /gene="rpmG"
                     /locus_tag="IWL30_002179"
     CDS             complement(14944..15093)
                     /gene="rpmG"
                     /locus_tag="IWL30_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP9706911.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(15114..15491)
                     /locus_tag="IWL30_002180"
     CDS             complement(15114..15491)
                     /locus_tag="IWL30_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706912.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(15523..15702)
                     /gene="rpmF"
                     /locus_tag="IWL30_002181"
     CDS             complement(15523..15702)
                     /gene="rpmF"
                     /locus_tag="IWL30_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP9706913.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(15717..15986)
                     /gene="rpsN"
                     /locus_tag="IWL30_002182"
     CDS             complement(15717..15986)
                     /gene="rpsN"
                     /locus_tag="IWL30_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP9706914.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
BASE COUNT         5153 a         3095 c         2452 g         5578 t
ORIGIN      
        1 aagcttacgc cgtctttcac tgcaaagact gttgttccac tttcaatact tgttccttcc
       61 cctgcggtga aggttacttt gtggtagcct gcaggaatgt tttctcctgg attttcataa
      121 atatctttac ttacagttac tgctaaatcg gtcgtgccta cagaaccatc ttggtaaaca
      181 attttaatag gaattgttcc agtaatcggc tccgattctc ctacaccttt agctggagtg
      241 aatgtaacaa taccagtgtt tgcatcaaca cttgcattac cgaagtactt attatagaca
      301 tctgaaattg aaaaggcatc tttagctaaa ggaacatctg gtttatcaaa tgtaggtttt
      361 ccactttgaa ctgtcgtttc tggagttgtt gttataggta cgtatgttgc atcattttcc
      421 atagcttgag aagcttttac agcaaactct acatccacgt cgaaagttcg atttccgatc
      481 ttaacagtta caataccttt cgtactatca gtaacgttag aagtatcagg ctctgttttc
      541 catgaatact ctgcatcttc aggtaagttt ttattattcc gaattgattc tttagcatct
      601 ggtttaaatc caactaccac ggtttgttta tcaaccgctt ttggtgattc cttaataacg
      661 gttgaacctt cttctggttt atcaaaacct ggagaaacga tttggatttt taattcatct
      721 ttcgcgatta atttacttga atctacaccc gtaaattcaa attctacagt atctgaaatt
      781 ggagccccat ctttttcatt tggagcgata gttttttctg ctaatacagt accttcttta
      841 tcaacaagtt gggctttgtg acctgttccc gctaactcgt ggatgaatac tttcccctta
      901 actgttgtgt caacatcata tacttcttct aattcaggtt tcccgatata tggattcgtg
      961 tctccgctct cttctttatc gtcagggttt ccatcaccat caatatcttg caaataataa
     1021 taacctgttc cataagtgtt ctggattaat tttttattcg tatccgacaa ccaagaatca
     1081 aagatgaaat cttcaccaaa tggtttattt tcttcaactt gtgctgttgc tttcatatct
     1141 tctaagattt catctaatgg cttagtcaat tgtaaaacat atcgaataac gactggacgt
     1201 ggacttgcat tagcaaaatc gataagttta gttaaatcat tattagttat tgaacctatg
     1261 ccataatttt catgaccttc ccgtcgctcg attgctaaat cagctacttt tgttccggat
     1321 tcaaacgaaa catcatatgt tactttgtca gcctgaacca tgtgcatctg tatacccgct
     1381 acatatggaa ctaatcgttc atcaattttg taattagcat accaaggcgt gccctttgcg
     1441 gaagatgaat atgagaaatt ataatttttc gttaacttat gatctaagat tatagctcca
     1501 ttagcacctg tggatcctga accatctttt gtttgagtat taggtttctc atattttgca
     1561 cttactgatc catgtttaaa ctggtcagga ttattttcag atacatctaa gctattatag
     1621 aaatcatctg aatctgttaa aaagtagccc gtactatcaa tacccggtac aattttatca
     1681 tcgtcttgat gacttaccaa gtagattcga tacattaaac gatcggtctt gagattgtct
     1741 atgcctatct ctttaaaaat ttcatctagg ctcttttcaa acgtaattac cccattagga
     1801 gcggtttgcg tatcggttgt ttctgcccca gcaaataatc catcacttgc acgaatataa
     1861 ttgaccttcc aaatatttgt aggtctaccc agcgaatcat taattcttac aaaggtccgt
     1921 ctagaagatc caattccttt ctttggatcg acctgaattt tttctacata tttagctaat
     1981 cgttcatcta attgaagacg gatttcccag tttttagttg atgctgcaat attatgtttt
     2041 ccgtaaactt caaatccaat tgtattgcct tttagtgtgt ctccagttag ttcttgagga
     2101 ctaaattgta aatccgaaaa gttataattt ttcgtataac caggattctc ctgaaaagta
     2161 ttccccgaag ctactttgtc acctacttgt acggagctta tcgctgtttc aagtgggctg
     2221 cttggagaaa tttcatcttg tgctttaact agttcagttg ttcccaagac aagaccaaca
     2281 ccaatcaaaa cagatgcaac cccaacactt aattttttga tactgtactt caatactcgt
     2341 ttctttgttt tttctagaaa tactctctta ttattcttgc taaccattat tcaaatcaac
     2401 tcccttaata attactttcc tagtaagaat aaaagataaa tctgatacgt attattcaca
     2461 gaataggaat cttactaacc ttatgttaac taatttactt agagaatttc atgaacatta
     2521 caacaaaaaa aagtacattt cttcatgttt ttttcggata tttgtgcttt ttaaccattc
     2581 agcagctgaa aacatataaa aagatatgta tgtattcttt ctgaaattaa gttcatttct
     2641 atgcgatgaa gcatgttttc aaaatattac cctttcttag attaaacttc agagtattca
     2701 aataacggaa taaggagcct tatcccgcat tactgaatga gactgtgaca taagtaaaaa
     2761 ggtgacttca aattccttct tcggaatttg aaatcacctt ctttataatt ataaagtatc
     2821 ggtttcacag catttctgaa tgattcctag aatcttaaac gaatgattgg agttatcaac
     2881 attttttcac tcgtttttag cgaaacaaaa aatgacattt gtcacagcct cattaaatta
     2941 ttgattcttt ttctttataa ttgaattgtc catatttaaa gtgtctagta ctctacatga
     3001 ataattaatt gctttcactc aaatacttat aatctattaa cttgtagttg tttaactttt
     3061 ctgaaaaagt tagacccacg taataatctt cccagctttc tctttcaatt aactgatcca
     3121 atttttgata atccataaat tcatcatgta tcaaaaagaa taaataactt gatattttag
     3181 catctagctt aatcagatta aataatgttt gtttaaaatt gctaccatta atatgactga
     3241 tagattctct agcttcttca cataaacttt ttactttatt aattactact ttgtcatatt
     3301 catttaacac actattatta ccattactta gacgctcaga ttctacgaaa caactcaaat
     3361 cagaaatttc ccaaaaaaat tttttgttaa aatccaattt cctcaactcc tccaaactac
     3421 gtaaatgata tagtctcata ataaaataaa acctctcagc taatgttgtt gaaattgtcc
     3481 actaaaccta attgatattg cttaggagat acacctgcgt actttttgaa tactctcgaa
     3541 aagtgtgaag aatctgaaaa tcctaaacct aaagaaactt cactgatagt atggtttgat
     3601 ttcaacatta ttttagccgt agaaattttt cgcttagaga tataattata aatcgtaata
     3661 tgcatttcat tcttaaagct ttctcttaat ttagtttcac ttacagaaaa ataattagct
     3721 atttccttta cagtaattct tttatagata tttaagccga tgaattgaac gacagaagaa
     3781 attaagggag aaagatgctc tatcttagtt tttccgattt ccttagtata ataaacaatc
     3841 ccagaatccc tcacttttaa gacctcaggt aacgatacag ctaattcgtt ttcttgtatc
     3901 aaagcatctc tactttgata cgcttctatt atatccattc ctaatgttat agacgtttgt
     3961 gctaactttt caaaaacaat gatagaataa tttttttcag accgaatcgt atctccacta
     4021 acacttggaa ttattccatt actcagatta aaaaccatgt tttctaaatt tcgaatatct
     4081 ccatttttaa catattccag tattttattt tcataaagaa acagatatat ttctggatcg
     4141 taattttgtg ataacagatt atctattttg atttgctcga tagttttact tagattttct
     4201 gtatattcaa ttaacggttc atggaataga tcttctattt ttccagtaaa aaaataatga
     4261 actaaaatca gaatatctcg aatgtctcct aaagaataca aagtcaaatt ttgcaatgaa
     4321 ttatagagtc gttctctatc agccattgca acattcaaat tgttcatttt tagtttaaaa
     4381 aaaactttat caatattatt tgctctaaat ggaccaaata agtaatatct ggttttaaac
     4441 atgtatagaa gaaacatctc attaaaatca ccagtaaaga aatggaaatc tttagttttt
     4501 ttaatattat cagttagtaa ctttttataa tcataaaaaa aataagtagt tctatctgag
     4561 cgatactctt ttataatact gaattcggaa ttaaaaacca cgataggtaa ctttaaacaa
     4621 acatgaagag actttaataa gacttcttta tctgaaaaca cttttgtttc tctctttcct
     4681 ttctacatca ttaaatagta ttttatacgt tatctaaaat actattcctc tgtcaaaata
     4741 agctaactta cttcattata aaatatccac ttatcgcttt catgtatatt ccaccgtaaa
     4801 aagtacattt tatcaccgag aactcatcat ttttcatcat gatgaatact atttacatat
     4861 atctagttca aagacaataa ttattatgat ccgttattat ctaagcacta tacagataat
     4921 aggaattttt cgtttctaaa tattccaatt tgggaatcca ataaaaattt tcgttcaaat
     4981 acgtgtctat tcgaactctt ttctagttgt taattatgaa gggggaagaa ataaaaaata
     5041 caatctatac acgaataaga atattatcca aaaggaggtt ttagaaaaga tttattatac
     5101 gtaaaaagct caagacattt tcttagccat aaggacatgt ttgatattta agaagatata
     5161 tgaattttct tttctaagta taaagataac tatccattat caactaaaaa aacatttttg
     5221 aaaataaaca tcagcagtcc tatcaatgca tgtgagctta tagaagttat tcgcagcgca
     5281 gcataattgc attagcttct tttcgtgttt tttgcctctt ttccctttaa atcttaatgg
     5341 aggaggtgtg caatatgtca gataacgacg aacgcgtaaa tgcccagttg attcgattct
     5401 ttgaaaaggt cattttgaac accgctaata cttattataa aaaacaagaa gaaattcaaa
     5461 aacatgaaca acttgatcaa ctcccaccat atttgtttac taaagatact ataaaattta
     5521 aacaaccgat taatgtttta aatgtgactc tccttgttga agatataaaa ttagcaaaaa
     5581 tccttccttt gttgaaagaa cctgaacaaa tttttcttat cgaaaaattt atctttgata
     5641 aaactgataa agaaattgga aaggttctgg ggattacaag acagggtgcg acaaatttaa
     5701 aacatcgact ttacaaaaaa attcaaaaaa tgctggttta atatctgcat aataactaat
     5761 tttgtatgct tctttgctcg ttctcctcct tcccaaagaa atagattata agtccatggg
     5821 aagaaggtag atttatgaat tctttaaaag aagatttcat tttagcaaag gctggaaatg
     5881 aagaagctgt tgaggcaatt ctcaaaagat ttagctcttt gattcataag caatcctgga
     5941 gaactggaaa atatgatcaa gattgttatc aagaatgtat gcttgcaata tatctagcta
     6001 tctcgaaatt cgaaattaaa gaataactca actaagggag tctgtgacat aagtaaaaag
     6061 atagtttcaa atttcgaaca ataaatttga aactatcttc tttataatta taaagtatcg
     6121 attttacagc gttctataat gattcataga atctaaaacg aatgatttta attatcaacg
     6181 ttttttaatt cgtttttagc ttaaaaataa cttttgtcac agtctcactc ctaaaattac
     6241 tttatttacg tatgaagtgg gcaggaggtc gaatccagaa ctgggctatg gtacttcaac
     6301 gaggacttct cctctagaat tgaaaagcat gcgatttacc ttccgtaacg acggtagatt
     6361 tattcaacta tgtgtccaat caaaatttgt ctgatttcaa aaatagtcaa gagaattttg
     6421 gttgttaaaa actcttatct atcaacttcc acagtttagt tgacaaaccc tgatactaag
     6481 ttgggctatg atacactttt cttgactatc agttacgtta ccggaaggaa tgaactctat
     6541 tgagaaaaac atatgatcgt aaatttaaac taaagatttc acaggatatc cttgagaaaa
     6601 aagtaactac caagaaaatt gctgaagaat acaatatctc tcgtccaaca atttcccgat
     6661 gggtttcaga atatcgtcga tacgggaaaa atgcttttgc tggacaagga aaaagattac
     6721 ctgataaagc agatttctac attcttgaac aggaaaataa acgactaaaa gaggaagacg
     6781 agtgttctac gaacacaaag atcgatacgg atctccaggg atcactcaaa aactttacga
     6841 tgaaggaatc gaaatcaata aacgtgttgt agctgttctt atgcgtgaaa tgaatttatg
     6901 cacaagagga tttcatcgac gcaaatcttc tcatggtaga gaaaaaacaa tcaaagaaat
     6961 ggtaaaaaaa tgttgaatcg ataatttgaa cagaaccaaa ttgatgctgt ttgggtgatg
     7021 gatattactt acattccatg taatgacggt cgcctctatt tgtccaccta tattgactta
     7081 gcaactcgta ttctacgtta ctatatggta gattcacaca tgaaaaaaga gattgtcgtc
     7141 cgtccattac aaatttatta aggagaattg cctacggtaa ttcattccga tagaggtagc
     7201 cagcatcggt cttttattta tcaagagctg ttgaacgacc accacatcac acattcagtg
     7261 agccaaactg ggactcctgt tgacaatgca gtaattgaag cgtttcatcg atcaatcaaa
     7321 tgagaattaa tcgactccaa caaatataaa agtaaaatag agatgaaggt gttaatccaa
     7381 gattatttag aagactacta cccaaacaaa agaatccata caaaatttat gatgactcct
     7441 tgacaatttg aagcgaagaa aataagggat ctataatatc tagtgttggt gacgccttct
     7501 gttgccaata ctttttcttt tttctaataa acgcaaacag acaccttaaa atcctttaga
     7561 ataaagtcga cgaaaaccat tcaaaggagt gtttcaagat gtcctattca gagtttacca
     7621 aagagttact agatattcta gacctaaatc ttatctttca tgaagatgcc tttcgcaaag
     7681 agcgaatcaa tgacgaaaca tgttttgttt ttgatggcac attgacctat caaccggagg
     7741 aatgctttca tcgccactat caaaatgaac aaacgatcat taaatagggc tggaaaaaag
     7801 tatccatctt attgaatgat gtcagtaact acaagaccat tctgcggatt aacaaacagc
     7861 gcttcaaatg taaacactgt ggaaagactt ttttagcaga agattcagtt agtgatcgtc
     7921 gctgctccat tgctcgcaga gtcaaacaag ccattcttga gctattaagc gaacctcttt
     7981 ctatgtcttt gatcgcacgt atgaagcaca tttctccgac cactgtcatt cgaatacttc
     8041 gcagccttcg gcctaaaacc atttctttga gccagctgtt accagaagtg gtgtgtttcg
     8101 atgaattcaa atcagtgaaa aatgtctcgg gagccatgag ctttgtcatg atggacggca
     8161 agacccatca actgattgat attgtggaaa atcggcaact caatccttta cgcgactatt
     8221 tcttgcgcta ccctataaaa gtccgtgaac aagtgcgtct agtggtttca gatttctata
     8281 caccctatcg cacattggtg aaagagtgtt ttccgaatgc ccgaatcgtc gccgatcgct
     8341 ttcatattag ccaacacatt ggtcgggctt ttaccaacca ccgaattcaa gtaatgaaga
     8401 cctttaaaaa aggggatcgt cggtcgaagc acttaaagaa atactggaaa ctacttcaaa
     8461 agaatgcgtg ggaattgaaa ggtcagcatc gctactggcg tccgtcattt cgagatcatc
     8521 tgacagaagc agagatagtc gatcggctat tatcttatga tgattcctta aagcgaggct
     8581 acgaagtcta ccaggatttt ctttccgtta tcagaagaca agatgttcct gaattcgtcg
     8641 cgctcctaaa agaagactac aaggagttgc cagagcacta ccaaccagtg ttcacgacct
     8701 tcaagaaata ccaaacagaa atcaaacgag cgttgcgcgt tccctattcc aacggcccta
     8761 tagaatgttt gaataaccat atcaaagtac taaaacgaat tgcctatggc tatagaaact
     8821 ttcaaaacta ccgagaacga atctttttat atcgaggaaa atattttaag aaaactaaga
     8881 atactaccca actgactaaa gccagaacaa caacacggct agacaagata gctgtctagc
     8941 cgtgtgagta tctcgattat tctattgaat ttcttcacca acactcattg acgaagagcc
     9001 aaaataagta atctaaccga ctgttaacta aaccattgca ggtcccgtta aagatacttg
     9061 tgcctgttgt caccgcttct ttttcttgtc cacaagcgct taacacactt aataaagccc
     9121 ctgctattat taataataat atctttttct tattaaaaac ctcctcatct atttccagat
     9181 tagtaaatgt agtaatctgt gctttctttt ttctcaatac taataactaa cttatggggt
     9241 aaacaaatac tcgtttgacc gggttttgag atccatccag ttctaaccgc gatttgatca
     9301 ggactattat cttctttaac tcgaattctt ccatctttaa tctcaacaat attatattgt
     9361 cccttcgcag gatgataatt cacgagttta tgattgatct tatccaaatc gaagcgatcc
     9421 atttccttgc catcaattcg aacgatggca tatttgatag catcttcagt tgtttcattc
     9481 tccttttcta tagagaatac aaataaggga ataaaagagg taataatcaa aatgccaata
     9541 attatgacat cgcctatctt gacaccagct tttcttaaaa atttcattaa cgatatctgg
     9601 ctaaacatat tagacttttt ccatggaaat ggtgaaattt ttcggaaatt atctgcatta
     9661 agagaatcag ttaaacaacc ctcaagttgt tccttaatac attgttgatc catcttcata
     9721 ccaataaaaa ctagtttatt aatgcgttca ccaaacaacg gatgccaggc ttccttcaat
     9781 tcgggatttt tttgtataat catttgacac tcaattttag gtaaactagc tacccaataa
     9841 tttaatgatt caatactttt atagctaccc gatgtttcaa agatcgtcga aaaatcacta
     9901 tgttgggcaa accaaagaaa cccttttgct cttgtaatat tgccaggaaa atttttatcc
     9961 gtaagatggg caaatttttg tgcgtcaagg agtaaccgat ttcgatagat aaaacttgag
    10021 ataccatatt cttctgtttc aggaatatga ttttgatagc caagctctaa ctctttgaac
    10081 cagcccactg aataggaagt ctcatctata ttaaaaagat ttgtatttag aattttcgat
    10141 aagtctactt cacctttaat tgtcctaatt attttggcat tggactgtaa cttagcaatt
    10201 agaccgttaa ttttgtaaat cgtcttggtt gacactaagt cactattatt taaaagtaat
    10261 atgttacaac actcgaattg atcaattaac aagccacgaa tatcctgttc ttcttcaact
    10321 acattacctt cagcttttct ttcctgaatc ttttcacccg tttcgaaatt atcccaaaaa
    10381 cgttttacat ccactactat aataatggca ccaatctcta atcgttgggt caaatcaatg
    10441 tctaaagggt ttttagctaa tataattgct tgaataattg gaattggttt gctgattcct
    10501 gaagcttcaa taacaatttg attcaagtca gaaaattgca ctaattgatc tagttcaaca
    10561 atcaaatctt cgcgtaatgt acaacaaatg catccattcg gcatcgaaac aagtttttct
    10621 ccagttcttt taaaaccttt cttgtcgact aataccccat caacatttac ttcccctatg
    10681 tcatttacaa tgacagcaat ttcctttccg tgatcaccgt acagaatatg atttagaagt
    10741 atcgtttttc ctgaaccaag atagccagtt aagattgtaa taggtatttt tttcataggg
    10801 aacctctttc tataccttga atttacactt taagtgcaac acataaaaat gagtcataat
    10861 cgatatccaa ctagtataag tttgtcgaga acactcctag aaggaaaata attatgagcc
    10921 agtatcgtca tcttactata ctcgaacgag aaagaatctt tctgcttcac gaagaaggat
    10981 tttctattag tataattggc aacgacattc agcatagccc gtttactatt ttacatgaac
    11041 tgagaagaaa caagaaaaaa actattcacc ttccaaagcg gaaaaaaatt atgcttatcg
    11101 aagaaaaaaa tgcggacgtt atcacattct aaatgatccg tgagtttggt caattgttcg
    11161 tcatttattt gtcgattttc agtggtcgcc agaggaaatc tcgaatcgtt tgaagcatga
    11221 acatatggac ttttcaatta gttttatcac gatctaacgt gcaatttgca atgatctttt
    11281 cgatacagaa cccctctcac acggcaataa aggcctgatc cgttcattgc accatcgagg
    11341 aaaaacaaga catacaaaaa atcatgtcga gtctcgtgga aagatttgcc ttactcattc
    11401 gatccatggc cgtccaaaat ccgcagatga tagaagtgat tttggtcatt ggaaagccga
    11461 tattgtagct ggcaaaactg gtggtacttg tttagtgact ttgacagaca gaaaatcatg
    11521 cttcctttta gcaggaaaga aggtccccag aaagaaagcc gcttatgtga gcgagaaaat
    11581 gattgaactt ttaagcagta ttccaaatga ggcggtcaaa acgatcactc cagatagagg
    11641 aaaagaattc tccaatcatt tcgcagttac agaagcattg aataatatgc ccttttattt
    11701 tccagatcct catgcccttg gcaacaatga acaaattaaa atacgaatag actcattcga
    11761 gaatacttgc caaaatcgaa agagatagct acggtagata actcaacgat aaatcagatg
    11821 attgaaaaac tgaataacag acctagaaag tgtttagatt ggagaacacc ttatgaagtc
    11881 ttctttgata aagtgttgcg cttgatttga caattcaaga cataataaaa agcagatcaa
    11941 atccaaataa gggaaaaatg atctactctg gtattatcaa ttaattggcc aagctattat
    12001 gccgttttgc taaacgtcta cttcttccag tattgacctt gataaaacaa caatcaggat
    12061 cccgttttaa tactctatga actgtgtcaa tactaacatg taaagttgca gcaatatcag
    12121 ctaatgtact cccatttaat ctcatttgct ttgcaaatcg ataacgccga taagcatcaa
    12181 tattcttgga atccatagac aataatactt ttcttaccat gtatttcctc caaaaattga
    12241 attttaatga gccatcattt cttgagcaat ttcatgttta gacaatgaag ctggatagaa
    12301 agtaggccaa ttatgcaatt cattatagag tttttcttga ctttctccac caaagaaaat
    12361 atggaaatgc tctgccttac ttggtgcaat tccatgatca ctaaattgaa tatatttaaa
    12421 acgaccagca cctgcatcat cagtttcaaa attaaaacga acaccacggt ttcctttttc
    12481 ataattcaaa attttgtatc caacatattt gtatgtgtat tgatgatgtt caccgttgac
    12541 tacaaaatca atggtgttgt ctttaatgtt gatagtatca atatctgtct tatagccagt
    12601 agtataataa tctttgtact cggcagctgt catgtcttta ttgatttttg ctttgtaatc
    12661 gaaaacttca tccaaaattc catcttttaa caacggataa acagattgcc attcgcctgt
    12721 ataatcactc aattcgcgat ccttcacatc ggcatcttta aagtacccat tagcaactgt
    12781 cttttcatcc ttcgcttctt tttctggaac tacctcttca ccagcaacca tagttgtttt
    12841 ttctaacgct tttagattgt cttccattac tgaaacataa tcttcaccat tatccatttg
    12901 ttcttttgta agactttcaa gaggattcag tacttccaat tttatcccag cttcgttggc
    12961 caaagttttt gcaattttat cattagcatt cttttcaaag taaatataat tgatcttatt
    13021 ttttttaaca tactctttta attcagctaa tcgaccagaa gatggctctt cttcaggact
    13081 tagtccagca ataggaactt gtttcaatcc ataatccaaa gctaaatatc caaaggctgc
    13141 gtgttgcgtc acaaaacttt tttgtttcgc ttctttaaga ctagtggtat agtcagcatc
    13201 caaccgtttc agtttagtca aatacttttc agcatttgtc tcaaatactt ttgctttttt
    13261 cggatacaat ttaactaact gatccttgat atttgaaact tccttaattg ccattttggg
    13321 tgaaacccac gtatgaggat ctagttcatg atggtgatct tcttctccgt gatcatgatc
    13381 ttcttcttca ctacctggca ataaaatcat gtcttttgtt ccttctacta cattaggccc
    13441 agctttcttc caactttctt tagcttttgg tacccatgat tccatgtttt cgttatgata
    13501 tacaaaaaca tcagcatcat gagcttttgc catatcctta gctgagggct catagtcatg
    13561 aggttctgat cccgctggaa ccattaaatc cacgttcgct tcatcgccga cgatgttttt
    13621 ggtgaaatca tacattggat agaatgttgt tacaatttct aattttccat ctttatcatt
    13681 cgatactttt gcatcattag tgctggagca agctcccaat aatcctagag ctgctatccc
    13741 aataagtcct acactgattt tcttcattct ttttccttcc ttaattatgc taatttcttc
    13801 attttatttc gtttatgttc tttgataatt tttaatgccc tttttctagt tttaatattc
    13861 gggcttttaa gtcgtcgata tgcactagat atatccgttt tctccattag aaattacccc
    13921 caatcactac ttatcattat ttgctttccg ccagtaattt atcaaaactc tgtttgatcc
    13981 tttcttcatc caacattttt ccgattagaa taatagtggt ttggtctttt tgtcgagtgg
    14041 tgtattgaaa attaacctgt tcgtttacct cttgcatggc aactataaaa tcctgctctt
    14101 gtaaagcaat caatccttta aaacgataca ggctcccttg gttggttaaa atcagccagt
    14161 caatccattg gagtaaaaaa tcttttttta tgtcttgatc tgaagttaag agaattgatt
    14221 taaactcatg tacttgatgg tgttgatgat agtatttgcc attatagtga ttttctgatg
    14281 aatcactttc aaaaactaag ggatgattaa attttttcaa attataaaaa tccttttttt
    14341 gaatcgtttc actatgtgtg aacattcgaa tatcggataa agggttaata cttttgattt
    14401 ctctctgtaa tttttttagt gcagataaat tcaagttttg ttttctagac acaaaaatgc
    14461 gatctgccat gacaagttgc ttaagggctt gtggttctct ttcaataagt tcaaaactgt
    14521 cactatcaat aacagtcaat aaactatcga tatatactct tcctcttagt tgaggcgctg
    14581 ttaaaagtgt ttgaatgatt ggttggggat ctgctatacc tgtagtttca atgatgattt
    14641 gtgaaattgg ttgttgatgt tcttctattg tttccagtaa agaaatcaat attgctaata
    14701 aatcactgcg aagactgcaa cacatacatc cattatttag ttgtaacacc cgttcctcgc
    14761 tatgaactaa taatttgtga tcaatcccaa cttcaccaaa ctcattttca attataataa
    14821 tttcttccct ggggtagggt gagacttgca aggcttgatt gattagcgtt gtttttccag
    14881 cccctaaaaa cccagaaacg attgtaactg gtatgcccat tttgtcacct ttttaccact
    14941 tagctatttc acttctttaa aaattgcccg tctgcgcaat tttggcgaat acttttttaa
    15001 ttctaaccgt tcagggttat tccgtttatt tttgctggtg agatacagac gttctccagt
    15061 ttcgacacat tccaaaataa tattttgacg cataaatctt cctccttcaa tacttatcga
    15121 tcttcaagat atgcttgaat ttgttgtgca tcttcaaaag ttaactcgcc agattccaat
    15181 tgtgaaacta aattttcttt catatcaaca ggtatctccc aataaccatc gatacattga
    15241 aataactcct cgtcatactc ttcaaaaatt ggatagattc ttaaaaaatc atcaaatgat
    15301 aagccttggt cctcatagta atctttgact aatccatttt caattgcctg ttgggtcggt
    15361 aaacccgttt cagtaataat tcctctctct accatcgaac gataaaaagt gcatttatct
    15421 agcgattgct gggtcgtttc aagataatac tttaatcctt cttcaactaa aggtgggtaa
    15481 ataatttcca ttttgattcc ctcctaaaag ttatatatta aactattttg aagctccttt
    15541 aattactttc tttccattat agtacccttt taaagataca tgatggctac gtctataatc
    15601 accattcgct tcgtcaaatg aaatttctgg ttttactaca gtttgatgag ttcttcttaa
    15661 ccgtttcttc gccttagatg tttttcttgc tggtactgcc atgatatttc ctccttttac
    15721 caacttgctt tttttactcc aggaatcaac ccttgatggg caagttctct aaacttaata
    15781 cgggacatac cgaactttcg catataccct cttggacgac catccgtctg atcacgaagc
    15841 ctcagacgat tcggatttga atctcttggc aatttagcga gagcggtgcg atctcctgcg
    15901 gcttttaatt cctgacgttt atctgcatac ttttcaatca tcgccatttg tttctttgct
    15961 ttagcaattt tcgacttttt tgccatatac ctttatactc cttttatttt gatttagaca
    16021 cctcaaatcg taatcgttac gttttgacac tgatacttta ccaacaagag ttgctcttgt
    16081 caacaataaa taacatgaaa taaaactcat atgaatagat aactatttga aaacgaattt
    16141 atcggtacat agagataatc aaatcattta aaagaatatg cagtttaggc tctgaaaaat
    16201 aagaattcac ttttattagt ccaatcaaac acaaataccg ttttaagtag cttttcctct
    16261 acttaaaacg gcaatatg
//
LOCUS       DACDUU010000066        16197 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.066, whole genome shotgun sequence.
ACCESSION   DACDUU010000066 DACDUU010000000
VERSION     DACDUU010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.066"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(20..955)
                     /gene="murB"
                     /locus_tag="IWL30_002183"
     CDS             complement(20..955)
                     /gene="murB"
                     /locus_tag="IWL30_002183"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAP9706915.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1028..1948)
                     /locus_tag="IWL30_002184"
     CDS             complement(1028..1948)
                     /locus_tag="IWL30_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9706916.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2061..2813
                     /gene="xth"
                     /locus_tag="IWL30_002185"
     CDS             2061..2813
                     /gene="xth"
                     /locus_tag="IWL30_002185"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAP9706917.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3677..4210
                     /locus_tag="IWL30_002186"
     CDS             3677..4210
                     /locus_tag="IWL30_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAP9706918.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(4700..5224)
                     /locus_tag="IWL30_002187"
     CDS             complement(4700..5224)
                     /locus_tag="IWL30_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9706919.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(5217..5690)
                     /gene="tsaE"
                     /locus_tag="IWL30_002188"
     CDS             complement(5217..5690)
                     /gene="tsaE"
                     /locus_tag="IWL30_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAP9706920.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(5813..6793)
                     /gene="pta"
                     /locus_tag="IWL30_002189"
     CDS             complement(5813..6793)
                     /gene="pta"
                     /locus_tag="IWL30_002189"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP9706921.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(6946..7626)
                     /locus_tag="IWL30_002190"
     CDS             complement(6946..7626)
                     /locus_tag="IWL30_002190"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP9706922.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            7752..8621
                     /locus_tag="IWL30_002191"
     CDS             7752..8621
                     /locus_tag="IWL30_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9706923.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            8647..9162
                     /locus_tag="IWL30_002192"
     CDS             8647..9162
                     /locus_tag="IWL30_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706924.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(9209..9772)
                     /locus_tag="IWL30_002193"
     CDS             complement(9209..9772)
                     /locus_tag="IWL30_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9706925.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(9827..10006)
                     /locus_tag="IWL30_002194"
     CDS             complement(9827..10006)
                     /locus_tag="IWL30_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706926.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(10083..10592)
                     /locus_tag="IWL30_002195"
     CDS             complement(10083..10592)
                     /locus_tag="IWL30_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706927.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(10688..11068)
                     /locus_tag="IWL30_002196"
     CDS             complement(10688..11068)
                     /locus_tag="IWL30_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP9706928.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            11398..12147
                     /locus_tag="IWL30_002197"
     CDS             11398..12147
                     /locus_tag="IWL30_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAP9706929.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSRGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKLTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(12331..12498)
                     /locus_tag="IWL30_002198"
     CDS             complement(12331..12498)
                     /locus_tag="IWL30_002198"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706930.1"
                     /translation="MKDIQSGNVQETIDKWKGKHYRFTEAQKQALEYYQKLEESLRFN
                     DEARKYLKELL"
     gene            complement(12514..13608)
                     /locus_tag="IWL30_002199"
     CDS             complement(12514..13608)
                     /locus_tag="IWL30_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706931.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSK"
     gene            complement(13687..14331)
                     /gene="pcp"
                     /locus_tag="IWL30_002200"
     CDS             complement(13687..14331)
                     /gene="pcp"
                     /locus_tag="IWL30_002200"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAP9706932.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(14530..15474)
                     /locus_tag="IWL30_002201"
     CDS             complement(14530..15474)
                     /locus_tag="IWL30_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAP9706933.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(15476..16162)
                     /locus_tag="IWL30_002202"
     CDS             complement(15476..16162)
                     /locus_tag="IWL30_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAP9706934.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         4662 a         3356 c         2866 g         5313 t
ORIGIN      
        1 ttttaagttc cattacttat taattggcta aacttctgct tgtttctgat attgcttgtt
       61 tttctccaat gatccgcact tctgtttcta aagacacatc aaatttttct ttgattactt
      121 cttggatgtg cgcaatcaat tcaatataat ctgtcgctgt cgcatgatcg atattgacga
      181 tgaatccagc atgtttttca gagacttgtg caccgcccca tttcaggcct tgcaatcctg
      241 cttgttggat cagctgacct gtatagtgtc ctactgggcg cttaaagaca cttccgcatg
      301 aagggtattc taatggctgt tttgattgac gcaattcggt caactcatcc attcttgctt
      361 tgattgcttc ataatcccct gtttgcaaac tgaatcgagc ttccaatacg atagcacgta
      421 attcttggac tttactgtga cgataagaaa ataccatttc gtcttttgtc aaagttttca
      481 atgttccatc ttcaagcaaa agatccactt cagcaaaaac atctttgatc tctccgccat
      541 acgcacctgc attcatataa accgctccac cgacacttcc cgggatgccg caagcaaatt
      601 caaatccagt caatgattct gccaaagctg cataagtcgt atcaatcagt gaggcacctg
      661 cttccgcaat gaccatcgtt cctttgacat gaatctgttt catttcagtc agcatgatga
      721 ctacaccgcg aatcccgcca tcttgaacga tcagattgct tgcatttcct aataccatcc
      781 aaggtatatc atggattcgg caataatcga tgatttgctt tacttcttct tgttttttcg
      841 ggaatgcaag gacatcagct ggtccacctg ttttcgtaaa cgtatagttc atcaatggtt
      901 catcaaacaa tatagtaatc gtggctaagc tttcaactat ttcttcttta ttcactgagg
      961 attttccttt cttttctcaa acgtcctcat tattctatca cgcttaatac aatcttgcca
     1021 gtcttgttca aagttgatga cggtagtgat ctttaagcac ttcctcacat aattcatgac
     1081 ttcttacaac ttctttttgt ttaagcaatt ctgtttccga tttacctaaa cggatctgag
     1141 aaaagaaggc ctctaccatt ggctcaaacc cgcgtttgac tagcgtgttt gtccagtcgc
     1201 caaattcttt cacttttttt ccatcagcat cttgaatgac aagctgggtc agatcgttta
     1261 cttcataagt accattttta cttgttactt gatatttttc tatattagcg ccacttgcta
     1321 aatccatcgt taaaatagcc gtttgatttt gtgtttctaa ttgtaaaatg gcatattcca
     1381 aaaattcctt tgtttctctt atctggtatt ttgttctcaa aatgggttca tctaataaat
     1441 acacagctgt atcaataaga tgtaaaaaca aatcataaag gacgaactct gtcgtttctt
     1501 ttgccgctat tcgatttttt tgcaggtgga tcaaccgctt atctgggata cgtttcaatt
     1561 cttctaccat aggagcaaag cgccggttga aaccgatcat caaaattatt tggttcttat
     1621 cagctaattc ttgcaattca cgaacttcat ttagatggac actcagtggt ttgtcaatat
     1681 aaacatggat accattttct agacattttt tggctaattc ataatgtact tttgttgctg
     1741 cgtggatcat acatgcttcg attttttctt ctaaaagctc atcgatcgtt tcatatagat
     1801 gttggaaccc gtactctttt tgaattcttt cttttgtttc tttatttctt gttgcaaaat
     1861 aaaaatcaag tgttccttgt tttttcgcat aagttggtaa atatgctttt tctgcgatat
     1921 tccctacacc gattacacca atcttcatct actttcctcc tggatcattt agttactttc
     1981 atcttacacc attttttact aaaatgaggt acaatagtat cgcaaaggta aggaaattaa
     2041 ttcgaaggag tgtgctttta ttgaaattta tctcttggaa cgtaaacggg cttcgtgcca
     2101 tcgtaaataa aaatttttta gaagtttttc atgagttaga cgccgatttt ttctgtctgc
     2161 aggaaacgaa actccaagca ggacaaattg atttagatct tccaggttat tatcaatatt
     2221 ggaattacgc ggaacgaaaa ggctattccg gcacagcgat ttttgctaaa aaaccagcat
     2281 tgaatgccac ttacggaatg ggtatcgata tccatgatac agaaggccgt ctgattactt
     2341 tggaatattc cgatttcttt cttgtaacct gttacacgcc aaattctcaa agcgaattaa
     2401 aacgtttaga ttaccgatta gaatgggaag aagcctttta taattatttg gaaaatctaa
     2461 aaaaacaaaa accagtcatc gtgtgtgggg atctaaatgt cgctcatcaa aagattgatt
     2521 tgaaaaactg gaaaacgaat caaaaaaatg ctggattcac tccagaagaa agagcggctc
     2581 tttctcgact gttggataac gggtttatcg atacattccg ttatttttat ccaacccagg
     2641 aaggtgtcta ctcttggtgg aactaccgat tcaattcaag gaaaaacaat gctggttggc
     2701 ggatcgatta ctttttgacg agcaaagatt tagagccagg actagctgat gcaaagatcc
     2761 atacggacat tatgggcagt gatcattgtc cagtagaatt agatttgaaa taaagaggtt
     2821 gtgaaaaagt cgtttagttt cgagtattaa gaaaaatttt gtgaaaatgg ctttccacat
     2881 tttgcataaa tttggcataa taccgagaaa ctggcttttg aacactgttt atcgagagat
     2941 tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca aacaaaaata gttcctcata
     3001 tttttcgttt ggttgggctt attaccgcag atacaagctt ttgaacaccg tttattaaga
     3061 ggttgtgaaa aagctgcact gcatcaagta ataagaacag ccaaacaaaa atagttcctc
     3121 atatttttcg tttggttggg cttattaccg cagatgcaag cttttgaaca ccgtttatta
     3181 agaggttgtg aaaaagctgc actgcatcaa gtaataagaa cagccaaaca aaaatagttc
     3241 ctcatatttt tcgtttggtt ggacttatta ccgcagatgc aagcttttga acaccgttta
     3301 ttgagaagtt gtgaaatcag cgtcctgacc tgagtattag agaaacaaat ggcaaaatag
     3361 ttcctcatat tttgctattt tttgagctaa taccgagggt cagctgattg aacaccgttt
     3421 agtggagaag ttgtgaaaaa gtcgcctact ttcgagtcgt aagaaatttt tttgaaaatg
     3481 gttcctcaca actttgcata aatttgactt aataccgaga agtcatgatc cccatctaac
     3541 tccaaatcac aaggaatgag ttcaaaaact agcttttctc aacaaatatt acataaaaaa
     3601 ttctatgttt tttgttaaat ttatttgctt ttttcaaaag tatgttacag taatttccca
     3661 aggaggtttt acagaaatga attttgttgc catggatttt gaaactgcca atcatcagcc
     3721 ttatagtgct tgttctcttg cgctagtcat ggtaaaaaat agccagatcg ttgatgaatt
     3781 ctatacattg atacagcctg agactccttt tttctggcga aatgtccaaa ttcacggaat
     3841 ccatcaagaa gacgtacgca atgcgccgaa attcccagaa gtctggcaaa ctatccaacc
     3901 atattttcaa aaaaatcgtt tagtcgttgc acataatgct gcttttgata caaaggtctt
     3961 agcgggatgc ttagattatt atcatcttga acagcctaat tatctttctt tatgtacagt
     4021 gaaaagtagc cgtcgcctgt ttcctgaatt tccaaatcat cgattgaaca ctgtgtgcga
     4081 aaatttgaac atccaattag atcatcacca tgatgcattg gaagatagtc gtgcatgtgc
     4141 ggaaatcctg ctttatcaag aaaaaaaatt cgggacagat ccattgaaaa aacttgtgac
     4201 tataaagtaa aagaatactt atgaaacaca caaagaggtc gtgagcctgc cgtttcactc
     4261 cgagtcacaa ggagcaattt caaaaaatag cttctcatgt ttattaaata ttgtgactgg
     4321 tgaccgagga gctggcagga gaacaccgtt tattggagag gttgtgaaaa agctgttctg
     4381 catcaagtaa taagaacagc caaacaaaaa tagcttctca tatttttcat ttggttggac
     4441 ttattaccgc agatgcaagc ttttggacac cgtttaccaa gaggttgtga aatcagcgtt
     4501 ttgacctgag tattagagaa acaaacggca aaatagttcc tcatattttg ccgttttttg
     4561 agctaatacc gaaggtcagc tgattgaaca ccgtttattc aaaaaaaatg gactgcgaca
     4621 cgacttttgt cacagtccct tcgtgtgttt ttctgccttc tcaaaaggat gacagtttct
     4681 cactcttttc ctatttcttc tattcaatca aataactcat tttatataca tctaaaaatt
     4741 cacctagatc tgttcttgcc cctctaggca tgaccgcttc gatttggaag cccatttttc
     4801 ggtaaaggtg tacggctctt tcattacgaa cttgaacgtc aagctccaat cgaaaaagta
     4861 cacccatttc cttcgcccaa ctgatgattt cttctagcat aagagtgcct agtcccattc
     4921 cccaatattc ctgcagaata ctgataccga cttctcccac gtgagacaaa cgaaattgac
     4981 tgtctgcctt gacagatgct gttccaataa tcgtcccttc atagattgca agcagcaata
     5041 aattattatt gctttcgtat atataatcca actcttcttc taaggtttct ggtggtaaca
     5101 ggagttctgc ttcatccaaa actaaaaatt ctgtctcctt attaacttgt cccatcatca
     5161 aaagaatgtc ttttgcatct cctggcactg cttcccgcaa gatgatccca acttcttcac
     5221 tcatgatttt cagcccattt ttctagaatt cgctgtttga aggtttcact ttgctccccg
     5281 tatgagatta gtttcacata ccgcttttct aaatctgtat cacttttttc taaaatcagt
     5341 tccaaataat cctccggcag tgcttcttct aatagatttc cccattcaac aacggaaagc
     5401 ccatcacctt caaaatattc atctaaccct aaatctgctc cgctagctgc tacccgataa
     5461 atatccatat gatacaacgg aatccgacct tgactatatt ctctaataat cgtgtatgtc
     5521 ggacttttga tcatttgcgt tatccctaat ccttgggcaa ttcctttcgt caacgttgtt
     5581 ttacccgcac ctagatcacc agtcaatatc agattgtcac ctggcattgc ggaatgacca
     5641 attgcctctg cgaattgttc tgtcattgtt aatcctgata gttcgatcat ttttcatctt
     5701 ctcccgctct tctttctttt tgccagtata tcgcgtaatg aagttttttt aaagaaaaaa
     5761 tgaactagtc aagaacagca gctgctgttt ttggctagtt cgcgtgtatt tgttaatcaa
     5821 tcaatgtttg tgctgctgtg ataatcgata atttatatac atcttcttca tttgctccac
     5881 gagacaaatc agaaaccggt ttgttcaagc cttgtagaat tggtccgatt gcttcaaagt
     5941 taccgaaacg ctgagcaatt ttatagccga tatttcctga ctgcaattct ggaaatacaa
     6001 agactgttgc ttgaccagct acttctgaat ctggcgcttt cagttgtgca actgaaggaa
     6061 cataagaagc atcaaattgc aattcgccgt cgatcatgta ttgtggcgct agttttttag
     6121 cgattttagt tgcttcaact actttgtcta cttctggcga tttcgctgag ccttttgtcg
     6181 agaagctcat cattgctact tttggttcga tatcgaacaa ttctgctgtt tttgcagaat
     6241 ctacggcgat ttcagccaat tcttgcgcag ttggattcac gttgatcgca caatccgaaa
     6301 agacatattt ttcttgatct cttccacgta ccatgatcat cgctccgctt gtccgactga
     6361 tgccaggctt tgttttgatg atctgaagtg ctgggcgaac agtgtcgcca gtcgaatgga
     6421 tcgctccaga gaccattccg tctgcaatcc ccatatacgt aagcatcgta ccaaagtaat
     6481 tcacatcttt taaaatcttt tctgcttgtt ctttggatgc ctttccattt ctacgttcaa
     6541 caaaagtatc caccatttca tcccacttat cgtaattatc agggctgatg atcgtaaagc
     6601 tagatgtttt gattcctctg gcatgcgctg catttcttat ttcttcttcg ttccctacta
     6661 aaatgggttc cacaagttct tctgctttca accgggcagc tgcgcctaaa atacgagcat
     6721 cagtagcttc cgggaaaacg attttgatcc cacggcgaac aattttaaat cttaaactat
     6781 caaacaattc cacaagatga cctccagacg tttttatatg tattatcata cactattcca
     6841 gaaaaaaaga acagtttatt ttatctgtat cagtcaaaaa tgctggaaaa attaagtttt
     6901 tcacatacaa agcgaactgc actataacag gaaagcgctg ttttattaca ctgtttcagg
     6961 cagttgccaa tcaatcggtt cttctcctaa cgcaaggagc gcatcattcg ttttcgagaa
     7021 gggtttggaa ccaaaaaagc cacgacttgc agataacgga ctgggatgtg gagatgttat
     7081 gatgatatgg cgactcttat cgatcatttt gattttttct tgtgccggtt ttccccacaa
     7141 gataaacacg attggttttt cccgttcatt taatttttca atgatcgtgt ctgttaatct
     7201 ttcccaacct ttcccgcgat gagaatacgc ttgcccttcc cgaacggtaa gaacggtgtt
     7261 gagtaaaagg actccctgtt tagcccatga cactagattt ccatgttgaa caggtgcgat
     7321 tccaagatca tcataaagct ctttatagat atttcttaac gatggcggga tctttacgcc
     7381 aggttgcaca gaaaaagata agccatgagc ttgattttct ccatgataag gatcttgtcc
     7441 taaaatcacc actttaactt cttcataagg agtcaattcc aatgcttcat agatatgata
     7501 catatctgga tggatctttt gggacgcata ttcttttttt aaaaattctc ttagatgcag
     7561 ataataatct tttgaaaatt ctgaggacaa aacttcttgc caactgttat gaatgatttc
     7621 tttcaaaaac tccacctcct aataaaaaga ataactcaca ttttcttcaa tcacaaggga
     7681 caatcttcac cctcagcact tgattaatat ggtaaactag gaacaagaag cagaaaggaa
     7741 cgataatcat tatgatcaaa ttaattgcat cagatatgga cggcacatta ttagatgccc
     7801 atatgagtgt ttctcctgaa aatgccgagg cgatacgttt tgcaaatgat gccggtgttg
     7861 agttcatggt cgctactggt cgaaactatc aagaagctcg cgcagcttta gatgaggtag
     7921 gaatcgattg tgcgatgatc actttgaacg gggcacaggt atttgataaa gaaggaaatt
     7981 cattgtttac cgtgtcgatt ccaaataatc aagcaatctc tgttttagat attctagatg
     8041 ctaacggtat ttactatgaa gtagcaacca acgatggtct ttattctgaa aatcaagcga
     8101 aacgaattga aagttttgct tccatggtgg ctacccattt acctcacctt acgtataaaa
     8161 tggcgattgc catggcctca gctaagctgg agttattgca catcacatac gtggacagta
     8221 ttcgtgctat tttggaagaa aagaatctgg aagttttaaa aattatttgt tttcatacag
     8281 aagggccagc tgtattaggt ccagttggaa agcagatttc tcttttaggc gatctagccg
     8341 ttacttcctc tggtcaaaac aatatcgaag tgaaccataa aaacgctcaa aaaggaattg
     8401 ctgttgccca tgtcgctcac gagcgtggga tttctttaga agaagtcatg acgattggcg
     8461 ataacttcaa tgatgtcagc atgctgcaag tagcaggagt cagtttcgca atgggaaatg
     8521 cagaaatcga agtaaaagat tatgccaaat atacaacgga cacaaacctt gaatctggcg
     8581 taggaaaagc catattgcgc gcgattgcag aagacttgta gttttatcta gaaaagaggg
     8641 atatagatgt cagaattttt cattcaggaa aagcagcttg gacgtgcaac tcggacaatt
     8701 gtcaaaaacg aaaaaggaca ttccctgttt ttgatggttg gtcgctgggg aacaaagggc
     8761 gatgcattgt ctctttatgc catgaatggc gatctcgttg ctcacatcaa gcagataagc
     8821 ctgacattcg gtacgcgttt tgagctgtat aaagagttcg aaaaggtggg aaccatgcag
     8881 aaaatattca attggcccgg tgacttttat tatattcgta gacttcactg gacagtccaa
     8941 ggtgatatct ataatcatca atatcaaatc catcatttca atcagcaaat catgaaaatg
     9001 gataaagcaa cacttttttc aggagattac tatgtattgg atgtctcaaa tgatgaagat
     9061 gctccagttt gtatttgtat tgctgcggtt ttagattact ggctctaccg taaagacaaa
     9121 agtcgcgata agcctgtttc ttaccgactt aacttaagtt aaaaagatgt acctgaaatg
     9181 cggaaatacg catttcaggt acatcttttt agatattctt ttgcatatga tcataaagat
     9241 gaccactgat catccgctga ccgacaactt tgaagtcatg gcgttcgtat aattttttag
     9301 cctttggatt tttttcatcc acacttagac cgatgacgga gcgattagct ttcttcgcca
     9361 atttaggtaa tgcttctaat aatcgcgaac cgatgccttg tcctctaaaa tcttctgata
     9421 cactgataga gtccaaatac cattcgttgg gaaacgtttc tttatcaatg aacaaacgtg
     9481 tagacgcatc tagctgatat ttctctaaga taggtaccag tggctgatcg atgattgatt
     9541 cttcttcatc tgtataaccg taagcaactc ctgcaacttt accgtctatt tcttctacga
     9601 tacctcgtga ataactgtaa cgataatttg gatcagtgat tgcttcttct aaaatctcta
     9661 aagtcttttg ttcccctgtc tgcttcaaaa atggcagttc catatctttt agaatcacta
     9721 agatcaatgg ggcgatttct ggagcatctt cttttctcgc aaaacgaatc atagtttcct
     9781 ctccttttct ccattctaac atgcagaaaa agaaatcgca aaatcgttat tttaaaaaat
     9841 cttcaatctt ttcagtaatg aaataaccat gagtaacgat tcttgccgct tcagttcggc
     9901 aataagggca gacgactggc atttctttat cattatgttc gactacatta ttgacttctt
     9961 cgtatgcaaa tttttttccg caattagggc atttcatttt ttccatgtta tctcaacttc
    10021 ctttcatctt tactctctta gcttactctt atattgtaaa aaagagaagc aatatccttc
    10081 ttctatgcag atatccatac ctgacggtcc tgttctccat ctattaatcg gactttataa
    10141 taaggacgtt catctttgac tagaagtgtc cactctttca cttttgcttt tttcccgtag
    10201 tagctgactg cttccttcat cgctgcttgc ggttgtttga actcttccgt tttttctata
    10261 tcaaaaagat gttctgcggc cttttccgtg tcatttcgta cggttgtttt cccagtggat
    10321 ggattgactg ctatgacttg tccttcacaa aaaaactggt attcatatgt tgcattcgta
    10381 ggattctcca aatctttttc aactgcaaaa tgaatagcag atatgttatg ttcttttgtt
    10441 tcgtttttta cgattgctgc tgcttttttc aatgagatcc gatattcctg gtcactaata
    10501 tcatccatgt gaaaagcggc attgagcagt cccgtcatac cggtaatccc tattacgatg
    10561 agggcagcaa caccaaacat catttttttc attcttcatt ctcctgtttc ttttttttaa
    10621 agaatatcaa gaaatgaacg aatgaaaaat cgaatgaaac gaaaaaaaag tgacaatttt
    10681 gttatcttta ttcgtgcagt tctaatggca atccgtctgg atcaaagaaa aacgtcattt
    10741 tttttcctgt aaaggtatct tctctaatcg gttctgattc gatccctaag gactgcaatt
    10801 ctgcaattgt ttcttcaata tcgtctactt tgaaagcaag atgacgcaag ccacaagctt
    10861 ctggatagtt tggtcgttgt ggcgcattag caattccaaa aatctccaat tcagcacttc
    10921 ctagtttcag atctaattta tagtcatctc gttctttgcg gtaattttct ctgattactt
    10981 caaatcctaa tttattaaca taaaaatcac gtgatttctg ataatctgaa acaatgatgg
    11041 ctatatgatg gatcgttgta aggttcatat gagcactcct attcttcgtt taagctcgga
    11101 tctatattaa tctccagttc cattgtaaat gtgtcagatt gaaaaaaata gctgtttttc
    11161 ctttttataa gaaaaaaggc aaaaaaaaca gaaagtaggt cctccaagac actcttcctg
    11221 ttcccaatat aataaatgaa agtaagaaat tatattttca agaaaacaaa cagaaaatta
    11281 tcataacaca aattatttat aattgaaaga ctagtttttt ttcaagaaaa gcatacaata
    11341 gattgtgtta gtaaatacag aacacactac atttagtgtc ttaaggagtc ctcatacatg
    11401 aagtcatatt ttcagttttt agctcatcag ctaaaaggct ggccgcaaca aaattattat
    11461 cttttctttt tcagtcgtgg ctgccaggtc atgactcttg tcaatagtcc catcacttgg
    11521 ctgtccgtta ttacatttat cggtaccacg cttggtgtat tgtgtatttt atccatcaat
    11581 gcggcaaaat cagtcaacgg tattttaggt attttgtctg cgatttgttt tattatcgtt
    11641 gggttttcag caaagaacta tttaagtatc ggtgaacaga ttgcttatat gatcacattg
    11701 gatatccccg tattgttaag taaagaatgg aatcataata tggcttcaaa aatccgaaaa
    11761 ttaactggaa aaagctggtt tatcgcaatc atcagtactt tccttgtcta tcttgtttca
    11821 ggattcctga ttggtcgtta tacagatgat cctcggccgt gggtagatgc cattgctttt
    11881 tctattagct tgactgctgg tatcatcagt ttcttgcgtt acaacaacca atattactgg
    11941 tggttagctt cagggcttgc tcaaatggtc ctttggtata tttcatttac gcatggatct
    12001 gcaagtttgg cgatgttcgt gaacagctct gtttacttgc tgaatgatgt ccttgcattt
    12061 accgtttcac cttggtataa ccaaaaagaa cgtgaacgta tgcaaaaaat ggaagaagaa
    12121 tactacaacg cacaacaaca cgcataaaaa aactgagcgt gggacaaaag taaagaacac
    12181 ttttgtctca cgctctaaac acgtataaac ggcggaatca gaagcaactc cttcggaaat
    12241 aagctgaaat tcacaaaaat ttgaaaagca attttcgtga atttcttctt atttcttgga
    12301 gttaaacgct tctgtcccag cctctatttt ttatagtaat tccttcagat actttcttgc
    12361 ctcatcattg aaacgcagtg attcttctaa cttctgataa tattccaacg cttgtttctg
    12421 agcttcagta aaacgataat gttttccttt ccatttgtcg attgtctctt gcacatttcc
    12481 agattggata tctttcacta ctagttgcca tttttatttt gagactgtac ggtctttctt
    12541 atcagcagat tgtatagctt ctcgttcttt acgcaatact tttaaaaaac catttctgac
    12601 tatccgagga atgctgtcga agaacccttc tgtaaaacca tagcgacaat attgcttcca
    12661 aataaataga tagatttttg gttggatgaa tcgttggttt ttcgcaaaat attccatctg
    12721 agcagtgact tctggtgttc gttgacgttt tgtctttctc aacacggaaa taaatgtttc
    12781 aaaataagca ttaggatgta ctggcacttc ttcatatacg atggattgcc aatctttacg
    12841 taaaaatgcg cgatagttga tcaagaattc ttctacatcc tttactaatt cttctgcaaa
    12901 gtcgtcaaat agttcttttt ttatctgtcg cttttttgac tgcccattga tgtgttcact
    12961 tattttcttg atttcatttt ccaatgcttg ataatccgct atgtaaatcg gcatttctac
    13021 ttggcgaaat tctgcgagcc gctgagctaa ttttatatcc ggtaaatatg gctgtttcat
    13081 ccgacgattt tggtacaaga caaaacagta tttttgccat agtccttcag gaaaatcgat
    13141 attcttctgc ttaagccata ataattcgtc taaggccaga tcaacttttg tcattccctg
    13201 atggatcgaa gctgccactg tcggagagac atgcaggtga tcggcaaagt ggttgatgcc
    13261 tagttttagg atctttccgg tttttggcga tttaacgatg tattgcactt ttaattctct
    13321 accacactca caatacaatt tttgctggct tcgttttgtt cgaaaaaacg gattgatctt
    13381 gaagtcagaa aacacccaat ctgttgcagc taaataatgt tcttccataa aacgagagct
    13441 gattagatat cggcggtgct tatcgactag ttcccgctgt tttttcgtta attttgcata
    13501 gatctcccaa cgttgactgc ttgtgtattg tttagaaatt ttgatgggat agcttttgct
    13561 gatttctttt ggaaaccatt cttcaaagac ggattgccat tcttccacgg ctgtttcttt
    13621 cctttctaat tacaaaaagc tggctactaa cataacgtta gcaaccagcc agtgtttctt
    13681 gctaaattag tgagttgttc cggcatttcc ggaaatatct tctttatttt ctactgctgc
    13741 ttcaactgca taagtcaaag aagaagcaat gtcttctaaa gacatagaag gcgtacccac
    13801 tggacgatcg atcacttgat cgggtgagaa tggcacgtgg atgaacccac ctcgaattgc
    13861 cgggaattgt gtttcgatca aatgaagcaa acgatacatg atatcattgc agacaaatgt
    13921 tcctgctgta taagagatgt acgctggtag accatgatct tgtacatttt ttgtcattgc
    13981 tttgattggc aatgaggtaa agtaagcggc tggaccgttt tcttctaaag ccgtatcgaa
    14041 tggctgattt ccttcattat cagggattct tgcttctgct agattgattg cgacacgttc
    14101 gaacgagact gctgaccgcc cacctgcttg tccgacacag atcacgacat ctggttggtg
    14161 ctgttggatg gcttctttga ctacttgact gcttttttcg aatacggtcg gtacttccac
    14221 tttgatgatt tcagcaccgc tgatttcatc tggcattttt tttactgctt cataggctgg
    14281 attcactttg tccccaccaa atggatcaaa acctgttact agtactttca tctgttgttc
    14341 ctccgtccga ttgatttaac atatggttat cttttatcat accattttcc ttgaacagcg
    14401 taaataaagg aaacaatagt ttaaataaca taacagatca aacttaatat attcttataa
    14461 aaaaatagtg ttccactaca atgttagaag aggttgcgac aattattctg tcacaacccc
    14521 tctcttctat tatttaaata gaatcatata agcaatttgg aaaactagca taaataacgc
    14581 aataaacaac tggtttttga tcactccgta tttgtctttc atctccagca tcgctacagg
    14641 cacgatattg aagttagcag ccatcggtgt cagcagggtc ccacaaaaac cgcaagtcaa
    14701 tgcaaccatt cctattaacg ctggattcgc tccatgacta aatacgaatg gtgcaccgat
    14761 cccaaccgtc atcacggtga tggccgcaaa tgcattcccc atgattaccg taaatatcac
    14821 catgccaaga gcgtaaatga tgatgccgat gttcacattt ccttctggaa cgatcgtacc
    14881 tactgcattg gaaatcactg tcccaactcc agcgcttgta aataccgcac ctagagaagc
    14941 taaaagcatt ggcaacatgc ttaacggccc tactgtgccc agcatatctg ctgcatcatc
    15001 taaaaatgta cttggtcgat tatctcgaga aaagaacatc aagattagca tggctgcaaa
    15061 aacaccgacg cccacgccga ctaaagcacc taaattggta aatagagcaa acaagatcgc
    15121 aaagacaccg atactcaaag ctggaataaa aattttcatt cctagtttgt ctgacatttt
    15181 ttccatatat tcttttgatg gaagccggct ttctcctttg ctgactttct tagcaattgc
    15241 tggtatggtc atcgcaaaga tcaagccacc gttcacgatt gccggaagcc atcttcctcc
    15301 tgcaataact acccctaaga ccgtccagaa aaaggctgac ccataacgat gaggattggt
    15361 tttatctgtt aaatttctta ctccagtgta aatcaatacg agtcccatca cgatataaag
    15421 gatctccagt aatttatcgc ctaataaaat atcaggattt gtaaaaaagt ccatcttatt
    15481 gcccctttcc tggataatat ttcttcgcta atttcttttc tttaagatag aagtagacac
    15541 ttgccacaac taaagccgtg atagccactg gaacttccac taaagcaagc tgtcccaatg
    15601 ttacgtcata tcctagatct ttcagtgttg attggactag caacgcccct gctcctccga
    15661 tgaatagtac ttggccaaag aaccagcaga cattttccat agctgaagcc atacctttta
    15721 gttcttcttc atgcttagga tggacttcta acccttttga ttcgatggtc cccaatgcca
    15781 tcggtaaaat gattggacga acaaatccag caactccacc aaaactcacg ttaaaagccg
    15841 caaagatcat tcgaaagatc ccataaatat ctacaatcat accagcagat actttcttga
    15901 atcgcttgat caaggaagcc gctgattctt tcagaccatt tctttcaagc gttcctgttg
    15961 ccaacataat gatgataaaa atcgccatcc cgcgattgtt tacaaaactt gtacctaaaa
    16021 gttctaacat tccttgaatg ccaagaccgc cagtcaaagc tgtaacaact aaagcaacca
    16081 ttacgatcaa tatcgagtct aatttaaatg caaacccgag aataacaatc aagacgccta
    16141 aaaggctaag ataattttcc atatattttc ctccataaaa tgacaatatt tttttaa
//
LOCUS       DACDUU010000067        16056 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.067, whole genome shotgun sequence.
ACCESSION   DACDUU010000067 DACDUU010000000
VERSION     DACDUU010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.067"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            226..894
                     /locus_tag="IWL30_002203"
     CDS             226..894
                     /locus_tag="IWL30_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAP9706935.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1324)
                     /locus_tag="IWL30_002204"
     CDS             complement(1094..1324)
                     /locus_tag="IWL30_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706936.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1451..2833)
                     /locus_tag="IWL30_002205"
     CDS             complement(1451..2833)
                     /locus_tag="IWL30_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP9706937.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2989..4176)
                     /locus_tag="IWL30_002206"
     CDS             complement(2989..4176)
                     /locus_tag="IWL30_002206"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAP9706938.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4183..5214)
                     /locus_tag="IWL30_002207"
     CDS             complement(4183..5214)
                     /locus_tag="IWL30_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP9706939.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5230..6684)
                     /locus_tag="IWL30_002208"
     CDS             complement(5230..6684)
                     /locus_tag="IWL30_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9706940.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            6859..7764
                     /locus_tag="IWL30_002209"
     CDS             6859..7764
                     /locus_tag="IWL30_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9706941.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7855..8532
                     /locus_tag="IWL30_002210"
     CDS             7855..8532
                     /locus_tag="IWL30_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP9706942.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8813..9709
                     /locus_tag="IWL30_002211"
     CDS             8813..9709
                     /locus_tag="IWL30_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9706943.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9764..11134)
                     /locus_tag="IWL30_002212"
     CDS             complement(9764..11134)
                     /locus_tag="IWL30_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9706944.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11372..12118
                     /locus_tag="IWL30_002213"
     CDS             11372..12118
                     /locus_tag="IWL30_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP9706945.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12115..12954
                     /locus_tag="IWL30_002214"
     CDS             12115..12954
                     /locus_tag="IWL30_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP9706946.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12955..13905
                     /locus_tag="IWL30_002215"
     CDS             12955..13905
                     /locus_tag="IWL30_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP9706947.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14046..14798
                     /locus_tag="IWL30_002216"
     CDS             14046..14798
                     /locus_tag="IWL30_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706948.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14817..15968
                     /gene="queG"
                     /locus_tag="IWL30_002217"
     CDS             14817..15968
                     /gene="queG"
                     /locus_tag="IWL30_002217"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAP9706949.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5206 a         3002 c         3230 g         4618 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt tgataccgtt
     1381 gactagttct tccatcgcaa aagcagtccc ccattttaat ccaaatggcc gatctcgata
     1441 ctcgttatag tcaatgaatt tttttgctgt ccgtctcatc ttttccacct tctaatccac
     1501 tcataccacc tgcatgcccg ccaacgagag aactacgtgc gatggcgcgt cctccttgca
     1561 agagactatg cgcatggatg attgattgaa agccgtattt ttttctaatc gtatcgacaa
     1621 caaaatcaac tttcgcttca tttatctgct tttcaggatc ttcaaaaagg ttaagctgta
     1681 aacctgtcgc gtatacgagg tcggaacagt tcacgcctac acgccgaata tcctggtagt
     1741 ggtaatgctg gtggaaaatg gtcaataaag catctgctag atctctgcta gcatttgtcg
     1801 cttcgatttt catttgctgg tgaaacccgg tcttgccatt tggatcaaga tagcctaaag
     1861 agtatcctac ccataagctg actacttgtg tttttgctgc agatcgtctc aagcgagtgc
     1921 cgacttggtc ggccatttct ttgataacga tctcgatttc ttctcgtctt gtataatctc
     1981 tatctaggat ttgactattt ccgatacttt ttgattttgg tgtatattct tgtcccaaaa
     2041 aacttcgatc gatgccccaa gcatgggcat aaagctgagt accgatgacc cctagcttgc
     2101 ttttcaagtg ataataatcc gcgtgagcta aatcataaac agaatgtata cccatttgtt
     2161 tcagacgttt tgctgttcgt ttgccgattc cccaaaagtc agtgatatct gagattttcc
     2221 ataacttttc tggtacatct tcataacgcc attcagcaaa catttgcttt ccgtcttttg
     2281 agccattgtc taaggctagt ttagcaagta gcgggttatc gccaattcct atagtcgtgt
     2341 agattccggt ctgctcaaac acatctttat ggatcagttt agccatgtcg tacgcatttg
     2401 ttttaccaaa gagtgccatg ctatttgtta catcaaggaa gctttcatcg acggaataga
     2461 catgatggtt cgcttcgtct gcatattttt tatagataga attgatttct ctgttctttt
     2521 ccatataata ggccatcctt ggggcaacaa tatataaatc acttggatag gggaagggaa
     2581 gatctctggc acggctgata ttggtgatgc cgtaagcttt ttttgctgca ggactggaag
     2641 ccaagattaa tccgctaccc cgtgtttctg tcgaatcgga cggataggac atgactacta
     2701 atttagttgt aaggggatca agatcctttt caacgcactc tacggaggcg taaaaagatt
     2761 tgcagtcaat acataaaata tctcttgaag gttctttttc ataaacaaat accggatttt
     2821 gattcgccca cataacaatc acctcttcaa cacaattaga acacacgttc tctttccgtg
     2881 tcaataaaaa acagcaattt tcaaatacga gcaaaaggct cacatttgga aattgctgag
     2941 aggttatttt tatctatttg tttttagctg agatcgttga tttttttatc aaacatgaac
     3001 agcttctgga tcgatctttg ttgtcccatt tccaatgtgt gcaacataaa attccggagc
     3061 atagccgaca actttcatgt attcatcata gacattgttt ttgaaggcag gtatattttc
     3121 ttctttgaca agagcaatcg cacagccacc aaatccagct cctgtcattc gtgcacctaa
     3181 gacaccttct tgtttttgag cagcatctac tagtgtatct aattcgatcc cagttacttc
     3241 ataatcatag cgaagagagt ggtgagaatc atttaacagt tgaccaaatg cttctaagtt
     3301 cccagcttct aattcagctt ttgccttcaa agtccgctga ttttcagaaa cggcatggcg
     3361 agctctgcgt atcaatgtat catcgccgat ttggtcaata gttgattcaa attcttcttc
     3421 atctaagtct cctaaagaag agataggcaa agttttttgc aaacgtgcca aggcttcttc
     3481 gcattcgcta cggcgttcat tgtattttga atctgccaat tcccgccgct tattcgtatt
     3541 catgatgacg attgcataac catctagttt cactggaacc atttcgtatt tcagcgtatt
     3601 tgtatctaat aagattgcat gatctagctc accgaagccg atggcaaact gatccatgat
     3661 acctgaattg acaccgatga attcattttc tactttttta cctgtttgga ctagttccaa
     3721 gcgatctgtt tttaaatcaa acagatcttc taatacaaca cctactaata attctaatga
     3781 agcgctactg gaaagtcctg caccattagg gattgttcct tccactaata actcaaaccc
     3841 atgatcgatt gagtgtccag cttcttttaa tttaaggacc attcctttta cgtaatttgc
     3901 ccagtttgct tttttatcat attctagctc atctaaagta aaactaatga caccgatatc
     3961 gtcaaagttt gtggaataaa ctaatatttt attatcgtct cgaggtgctg ctacaccttg
     4021 cgttccataa gtgatggaag cggggaagac atggcctcca ttgtaatctg tgtgttcacc
     4081 aatcaaattg atgcgtcctg gtgagaagta ttgtgtagtt tttttaggtc cgaatgtttc
     4141 ggcaaatttt gtgtttaagc ttcttttgat ttccatgatg agttattcct ttcttgtttt
     4201 gattttgaat tctgttactg tttcaaatac tgatccggct tttaaatgaa tcgaaccaaa
     4261 tgctgagaag cgctctgcac ctggagctgt ttgagtctca aatgtgatgc caccgtgatg
     4321 tgccagtttt cggttgcgca tttctggtgt ttctgtgccg aaatttgcag tgaaaataac
     4381 cacactagaa gcatctgtag caatatctac ttggattctt ttatcggggc tagtcaagcg
     4441 agcggcttct ttacccaagc cggtttcttt taagaaaaat ggatgatcga ttccgtcaac
     4501 aagatttttt tgatccaaat cactagcaaa aacggacgct aattttttag gtatttgaaa
     4561 atcgaaagca cttcctgcag cattttcttt tacccctata ggaatactat ctgttcgcaa
     4621 aggagcataa gcttcgctat tgagccaaag ctcatgttga tcgattgact gagaagcatc
     4681 tcccgtcaag ttgaagtaaa catgatttgt cggattgaat aaagtatctt ggtcactggt
     4741 gccgcgagag gtcactcgcc agatattatc ttttgtcaaa gtatagcgga tttccacatc
     4801 cattgtacct ggaaaaccat gttctccgtc gggacttgta gtagtaaaaa tgacagaagc
     4861 ttcattttcg ccattcaaaa tgacataaga ccattttttt aattcaaatc caggagcacc
     4921 accatgcaga ttatgtccag tttttggatc tgtggctaat tggtaagttt ttccattgag
     4981 agaaaagcgg ccattttcga ttctccctgc ggtgcgaccg atagaagcac cgatatacgg
     5041 atctttttcg atatattctt cagcggtgtc aaatcccaaa actaattctc gatcatttga
     5101 ttttaaactg acgattctag cacctaaatc agagatagaa agtgctagct tgtttttgtt
     5161 ctccaaggtt atcaagtgat aaccttggcc aaaatctttt tctgtaatct tcattttcaa
     5221 gatcctttct taatgggaag gaactactgc atcgtgaagt tcatcttcca ttgtatcagc
     5281 tggagccaat ttgttttcca atttctcaac gatttgtgca tgttttttct ctgtcaatgt
     5341 aatcttccaa gcaaaaataa tagcggagaa aatcatcaaa gcagcaggag catagaatgc
     5401 ataagctttg aaagtcgtta cgccatgagc agtgatatca gcagcagtag cattaccagt
     5461 catcccagaa gcgatagcga caaaccctac gatcccgtta gacaaagcac cagcgatttt
     5521 atctagtaaa gggcgaattg atagagtaac agcttcgtta cggataccat ttttccattg
     5581 accatattca acagagtctg tgatcgtcat caaagcggat aagaaaatca attgttgagg
     5641 gaagtagaac aatactagcc cgatgataac gattggcaaa cttgaacctg cgatcgtaaa
     5701 gaaaaagtaa gaagaaatca tcattgcgat ccctccaagg taaacgtagc gacgagagat
     5761 gacgcgtgta agcattggga atagtggaac agctactaaa ccaagtaagg cagcaactag
     5821 accaacgatc caaaattcag ttggttggcc aagtacatat ttgaagaagt agaataatac
     5881 ggcagttgtt gctacattgg caatcgcata agcgatataa gcaagggcaa gccacatcaa
     5941 ctgatcattt tgagcaactg ctttgaacac atctttgatc ccagcttttt cattttgctg
     6001 acggatcgca gtatcgactt cttttgtgcc caaggcagtg ataatcgcag taccgcctga
     6061 aataacagca gcaatcacac cgaaccagaa ccagcctgaa gcgccttcaa tatgtttatg
     6121 agtaacaaga taggtaaaga aagtaacgat agggacaacg actagagtag taccatttgc
     6181 acctagagaa gagccgaaac gagcaaacgt agcaaatttt tcacgttcct ttgtttccgt
     6241 actcaaagct ggaatcattg accaaaaagc gatatcctta aatgaataaa aactatccaa
     6301 taagatgaaa acgattgcaa ataatatgat aaataaagtc gtattatgag tagctaagcc
     6361 aaagaaattg gtaaagattg ctaccaagag tacggaacta acaacgccgc cgataactaa
     6421 ccatggtttg aatttaccgt attttgtacg agtattatcg atgatgccac caatcaaagg
     6481 atcaaaaata atttcaacta agcggatacc tacaactaag cttgtaactg tagcaatcat
     6541 ttttgcattc gttgccttat ctgttcctgc gaacatctgg cttgtcacga ataccataaa
     6601 ataagtgctt aatgtaacat agaatgtgtc gtgtccaaaa gcgcctaaag cgtaggacac
     6661 acgttggatc attttgcttt tcataatctg tattcctctc ctgttcaata tataatacta
     6721 agtaaaccgg ttacaacgaa taacaaagat cttttaatga cttaaactta acaagaaatc
     6781 gcttcctttt taatatattt ctgtgacttt tatttaactt tttgttgcat tatactaaag
     6841 gagtaaggag ggatagacgt ggagcaatcg atttttttaa aaaaggaaca tcaacaagta
     6901 gaaaaagaat tgtattttga tttttgtggt ttctctaaaa cgatgccgtt tcattctttt
     6961 ggtcctgcta ttcgcaatga ttacatcctg catgtcgtga tggaaggcaa aggatcttat
     7021 catgtgaaaa atcaacaatt ccaattgaaa aaaggtgatc tgttcttgat tcgtccagga
     7081 gactcgacgt tttacttatc agatggagaa gatccgtgga tgtattgctg gctttcattt
     7141 ggtgggagtg ctgcagaaga aatcattcgg cagtcgcttt tcagagaaga ccaatacacg
     7201 atggtctctt ctgacataca aaaatatatc gacattattt tagaatgcat gaaagatacg
     7261 gaagaaacct tggaaaatga attacagtta acagcattaa cctaccgttt tttatcagtc
     7321 cttttaaaag atggcggaaa ggtcagcggt ggtgtacaaa aggttcattc ctctttagct
     7381 gttgaaacag tcaaatacat cgaggcacat tatgctgaaa aattgtctgt tgaggaaatc
     7441 gcccagcatt tagcggtcaa tcggagccat ttgtcgcgag tattcaaaaa tcatatgggt
     7501 accagtatca aggaatattt gatcggggta cggatcaatc gaggggcgtt tcttctttct
     7561 ctgacggatg aatccgtgga aagtattgcc tatcaagtag ggttcaatag tctagtcgta
     7621 tttagccgaa tgttcaaaaa gagtaccgga gaaacggcta cgaactatcg taaacgtatg
     7681 aaaaacgaaa attttaagaa tattgctttg gataccttga aaaaacagtt ggaaaaacaa
     7741 accgcagttt cttggacaac ctgattagtc tggttaaaat gacaagcatc ggcaaaagaa
     7801 acttgtggcg gttatgctac actgtaaagc gaagactaaa aaggaggaaa agggatgcgt
     7861 acacttatca tcattagcca tccaacgatc gaagaatctt taaatcagca atttttcaaa
     7921 gaagcttctg ctggtttacc agctacttgg catcatttgg aatcaagtta tccagacgga
     7981 aaaatagata tagagactga actacagctc ttggcccagc atgatcggat cattttccag
     8041 tttccgtttt actggtatag ttctcctgct catttaaaaa tttggcaaga taatgtcttg
     8101 gaacgagcag cacatgtatt aaaagggaaa gaactaggaa tcgtattgac aacaggtgtg
     8161 gcggaaaaag aataccaagc tggaggaaaa gaagaatata cgatttccga atttttgagg
     8221 ccgtatcagc gaatcgcaaa taaattccat atgacctatc ttccgccatt cgttttagca
     8281 caatttatgt atttatccca ggaaaaacgt tgggagaagc taattgctta tcagcagtat
     8341 ctctcattgg aaggcaaacc atcgctgact cagcggattg actggtttat tcaaagagta
     8401 caggaaaatc agaaaatgca ggaagaagaa tctgaaaaac aagcatatat cgtagaagca
     8461 ctgactgagg caaaagagca gatcgaagac ttgaccttta cactgcaaga aatgaaagga
     8521 acttcattat gacaaatgat tggctgatcc aacaattaga tgaattaagc gaaaaatcag
     8581 aatatcaaga ccgtgcgctc ttatatgaaa taaagaaact gatggctgag atagacgaac
     8641 gaacaatgca gctgcaagga gaaatcgacg gacaatcttg gaatcatctc aactggtgaa
     8701 attttcttat aaataacaaa gataaaaatg tgcacagcga caattatttg ctacaataag
     8761 aatatgaagc gtttgataac gctattatac ataaaaattg gtggtgaaaa aaatgtatga
     8821 tgaaaatgag tggggagaaa atgttcggat gacccgaaga agacagcaaa aacaggcagc
     8881 tcgtaagcgg atgatcatga ttggggcagc agttgccgtg tttttgatca tagcaggagg
     8941 agttgcgggt ctatattttc atggaaaaaa ttcgtcagaa gcgaaaagta cgaccataac
     9001 ggaaacaacg acaaagaaaa cagagacgaa acaaacaagt gagtcatcaa ctaaaacaaa
     9061 agagacacaa acttctgaag aaaaaacagc aaatggtttg ccagagcaac ttcaagcaat
     9121 cgtaaatgat tattcgaaaa agatcgaaga aaagacgcct gcattgattg aagaatacca
     9181 gacagaaatt cagggaaatc aagagggaat agctggttta tcagcagttg ctaaccaaaa
     9241 agcacgtgag cttcaagcaa tctccgacga agggatcaga aaattacgtg cagcgtatca
     9301 ggcagcagaa aataaagacg gtgtagactt agatactttg atcaatcaat tatcggctaa
     9361 ttatacgaat catgtcgcta agatttccga tatttatttg cagactagcg cctctcttca
     9421 agcagagtca acaagcacac aagacacaac atcttctgat tctgagacag cagagtcggt
     9481 agaagcttct gaaatgcaag ctcgaacaac acaagacaca acagattcct ctgaaactaa
     9541 taccaacagc gcgacttcac aggctgcgac aaccgtagtt ttggaaggag aaggtccaaa
     9601 tcaaatagcc gaacgaactg gcgtaccagt agaaacgatc ttgtcgctga atgggatgag
     9661 catggagaat tatttcctga ctcccggtga gacgctaaaa ttgaattgat aaaagcaata
     9721 aaaaagaacg aagcttccac taaggtgaag cttcgttctt tttttatttt ccggttcttt
     9781 tttcgcgtac aaagtaatgg atcgtcatgt aaagcaagaa aacgagtcca ataatcaatg
     9841 agattctcgt ttcaggattg ataaacatga aaatcaatac gatagctaaa aaagcaagtg
     9901 tcacataatt cgtataagga gatagtggca ttttaaaagg atgatcatgc attttatctg
     9961 cgtgcttctt tttgaagcga atctcgctga tcaaaatcac tacccaagga accataccag
    10021 gcaaaatact tgagctgtat acgaaaacga aaatattttc tgactggttg atgaacattg
    10081 gtagaagata atttaagatc agaccaatta agattccacc tgagattgct acgacaggat
    10141 aaaaaggcgc accgtgacga gatagtttca gaaatttttt agacatcttt ccatttatgg
    10201 caagtgtata gaccatacga cttgcgctga aaatccctga attgcaacct gataatgccg
    10261 ctgtaatcac gacaaaatta atcagtcctg cagcaaatgt aatgccgact ttagaaaaag
    10321 tttgtacaaa aggtgaacca atctcactca attgattcca cggatagata ctcacaatga
    10381 taaaaatagc accaatataa aaaatcaaga ttcgaccgat agtggactgg acagcttcaa
    10441 tgatcgtctt tttaggattc tctgcttcac cagcagtcat tccgatcagt tcgattcctt
    10501 gataggaagc aacaacgatc gataatgcaa agaaaaatcc tttgacgcct ccagtgaaga
    10561 atccaccatt tttccaaaga tttgaaatcc ctactgcctg accatgatta ccaaatccaa
    10621 ataagattac cccaagacct gcaatgatca tcaaaatgat cgttatgact ttgatgatcg
    10681 aaaaccaaaa ttctaattcg ccaaacgcct tgacagaaat aagatttgcc agcataagaa
    10741 acaagacgac aatgattccg ggaataattg ccggcaaatc aggccaccaa taattcatat
    10801 aagatcctac agcaatcact tcacttattc ctacgacaat atattgaaaa atattactcc
    10861 aagcggtcaa gtagccagcc acaggatgga tgtactcgct cgcgtaattt gcaaaagaac
    10921 cagtcgaagg atcaacgtaa agcatttcgc ctaaagcacg cataatcaaa tacaaaaaga
    10981 atcctgcaat cgcataggct cccatcactg agggccctgt ccaacgtatg gttgaggagg
    11041 cacccataaa cagaccgaca ccgatcgttc cacctagtgc gatcatttgg atatgacggg
    11101 cactaagact tcgagttaac ttttcttctt ccatacaaaa actcctttcg ttgttgagtc
    11161 aacaagaaaa aataatcaaa cgtttcaaca catgtttttc attattaggt tagccttatt
    11221 gaactcttga tgtgattcta cagaaaattt tcataaaatg cgaggaaaat tcgaaaaaaa
    11281 ctttttcatc attctacttg aaaatgcttt ccattttagt taaactatat ttaaataaaa
    11341 agttaggtaa acctaaaaag gagtgtaaga tttggaacgt acggccattg taattcaaga
    11401 cctttcagcc gcttatcaag ggaaaactgt tttgaaaact atcaacttga cgataaatcc
    11461 acaaaaaatt accggtatta tcggaccaaa cggtgcagga aaatcaacat ttatgaaatc
    11521 tttgctagag ttgataccag ctactactgg taaagtcacc ttttctggac agtcggtaaa
    11581 ctcagtccgt aaaaaaatag cctacgtgga acagcgaagt gaattggatc tttcctttcc
    11641 aattgatgta ttaggcgttg tacttttagg aacatatcca tctttacgaa ttggacaaag
    11701 acctgggaaa aaagaaaaag aacgtgcaag acaagctttg aaaaaagtag ggatggaaga
    11761 atatgccaaa agacagatca gcgaactatc gggtggacag cttcagagag tttttattgc
    11821 aagagctcta gcccaaggag cagaatggat ctttttagat gaaccattcg tagggattga
    11881 tgcgttaagt gaacgaaaga tctttgacat cttgcaggaa ttgaagaatt caggaaaaac
    11941 gattttgatc gtccatcatg atcttcataa agtagaagaa tatttcgatg aggttattct
    12001 tttaaataaa cagctgatcg cttccggtcc agtacaagaa gtctttacat cgaagaacct
    12061 tcaattggcc tatggtgaga tcatcagaca tttagtaaaa ggaggagaga aaaaatgatc
    12121 caatccttta ttgatggatt aatgaattat caattcctgc aatacgcttt ggtcacttcc
    12181 atgcttgtag gacttgcttc tggagtcatc ggttctttta ttattttacg agggatgtct
    12241 ttgatgggag acgctatctc tcacgcagtt cttccaggtg tagccatttc ttatatgttt
    12301 ggttttagtt acatctttgg agccactgct tttgggatgc taacagcagc agtgattggt
    12361 ttcgtgactc agcggagccg attgaaaaat gatacagcga ttggaattgt ctttagctcc
    12421 ttttttgcat tagggattat cttgatttca tatgcccaga gtgcgacaga tttgtatcat
    12481 attttatttg ggaatgtctt agctgttagg gaaagcgatt tgttgctgac agcactcgtt
    12541 agtggaatcg tcttgatatt tgtctttttc ttttataaag aattgaagat cacatcattc
    12601 gatccgacaa tggcaaaagc ttatgggctg aacacatcgc tgatccatta tcttttgatg
    12661 ttctttttga cattagtggc tgtagtcagt ttacagacag taggaacaat cttggtgatt
    12721 gccatgttga tcacaccagc cgccacggct tacttgctaa cgaaccattt actgaaaatg
    12781 atcattacag ctgcaggaat cggtatgcta agtgcagttg tcggtgtgtt tttcagttat
    12841 agttacaatt ggccatcagg agctacgatc gtgttagcat gtaccgcatt ttttatcctt
    12901 gcttttttat tttctccaac aaaaggaatt ttatttcaga aaggaagcag atagatgaaa
    12961 acaaaaaaat cactcttttt aatcttggca gttagtttct tggtattagc cggttgcgga
    13021 aagcaggctt ctgaccaagc ggatgaagga agtaaagaaa agttgtcagt cgtggctacc
    13081 aattcgatct tggcggacat ggcaaaagag gtcggaacag atcaaataga tatccacagt
    13141 atcgtaccag tcggaacaga tccgcatgaa tatgaagtac taccagaaga catcaaaaag
    13201 gcaagcgatg cagatgttat tttatacaac ggtttgaatc ttgaaacagg taacagctgg
    13261 ttcgataact tgatggaaac ggctaaaaaa gaagaaggaa aagattattt tgcagttagc
    13321 aaaaatgtag aacctcttta tttaactagc ggtgaagaac atacaaaagc agatccccat
    13381 gcttggctag acctatctaa cggaataaaa tatgtggagg aaatcgcacg tatattctct
    13441 gaaaaagatg cagaaaatgc gacactctat aaaaaaaatg cagaagcata tgtggaaaaa
    13501 ctaaaagaat tagatacaca agcgaaggaa agttttgctt ctatcgaaga gaacaaaaaa
    13561 ttattagtaa caagtgaagg ggcattcaag tatttttcac gagcatatga tttgccagca
    13621 gcttatatat gggagatcaa tacagaaagt caaggtacgc ctgatcaaat gaaagcgatc
    13681 attgatcaga taagagcaaa agaagtaccg gttttattcg tggaaaccag tgtagactca
    13741 agaagcatgg aacgggtagc aaaagaaacg ggtttgaaaa tctatgataa actgttcact
    13801 gattccatag caaaagaagg agaacaagga gattcttact atcagatgat gaaatggaat
    13861 attgaaacga tccatgaagg tttgtcacaa acaaaagaaa gctgaaaagt gcaaggtcta
    13921 gcttgaggag cagaccttgc attttttata agagatatga aaaaaaatta tgctgttttt
    13981 cgtcgtacag ataccttttt tgaagaaatt ccgttatagt tagagtaagg aaggaaggtg
    14041 tggctatgaa ttttacacgt aaagataaat atacgattaa agaagaagtc tttcgattga
    14101 tcagccagca ggaaaatacc gacaaggatc aagaccttca atttcttgaa gcagaattag
    14161 ctaaattatt catagcaaat caaaatgaga caacagaacc agatgaagag ctgtacatag
    14221 gcaaatggtt gtttgatgaa tttcagcctt tagatgaaaa taccggtatc ggttattttg
    14281 tgttcctttc tccagtcggc ttcacacctg gcgtattagt taaacaaaac gacgaaataa
    14341 ccgtcaaagc atggcctgca ttattttcag tcatcaaagc agaaggagta atcaaaaaga
    14401 tccagtcggc ctattataaa agtcaattta ttgccaacag caaattaatg aatatgtttt
    14461 ataatcgtct agtagaaatt gctaggcctg ggaaaatccc gcaaacattc acgtacacac
    14521 agcaaaaaaa tctctatgaa gaagtattgt attcccgcga tctgtctgag ttttcgccat
    14581 gggaagaggt aaggctccat gaactgttaa gtacaaaacc agtttcattg aattttttta
    14641 atgcattaga taacaaaaga aaattaattt caacaaatta ttgggaagta tttgctgcaa
    14701 gaaaaaaatg gatggaagaa aagaagattg ccgatcagca gatccagttt gtccagctga
    14761 aaaaagaaaa actatattat gggttataca gccgttaaca gcaagcgagg aagaccatga
    14821 cattaaaaga agaaatcata caagaaagca aacgattagg aattgacaaa atcgggttca
    14881 ccacggcgga accttttgat tcattaaaag aatcgttgga agaacaaaaa gcagctggcc
    14941 atacttctgg ctttgaacat ccaaatatcg atgagcgtct ttatccagaa atgacatttg
    15001 atcagccgaa atcgatcatt tccattgcac ttgcgtatcc tactaggatc catgaagaag
    15061 tgcctaggga tgaaaaacgc gggcagtttg ctcgtgcttc ttgggggatc gattaccatg
    15121 atatactcag agaccgtttg aaccgattga tcgaattcat ccaaacaaga gcagaaaaat
    15181 ggcaaaaaga agaggaatgg cgtctagctc cgcaagtaga tactggagaa ctgatcgatg
    15241 tcgctgtagc acagcgagct ggtttaggat ttattggacg aaacggattg ttgatcactg
    15301 aagagttcgg ttcatttgtc tatcttggtg agatcgttac taatattgta tttgagcctg
    15361 ataaaccagg agaatttggt tgcggtgact gtatgcgctg cgtgactgct tgtccgacaa
    15421 aagcattgct tggtgacgga agaatgaatg cccagcgatg tttgtcttat caaactcaga
    15481 caaaagggat gatgccggaa gaatatcgta aaaaaatgcg aaatgtcatt tatggatgtg
    15541 atatctgcca gttagtctgc ccttacaatc aaggaaaaga ttttcatttt catgaagaga
    15601 tggaaccaaa agtagacgaa atatatccaa aattaaagcc aatgctttcg atgtctaata
    15661 aagacttcaa acagcaattt gggcatttag ctggttcatg gcgtgggaag aaacctttgc
    15721 agagaaacgc attgattgct ttagctaatc taggtgatcg aacggcattg cctgagattt
    15781 cagaatgttt aacagatgtc cgccctgtca tcagaggaac agcagcttgg gctattggac
    15841 gtctaggaac aaaagaaccg gaaaaatggc ttgatgtatt agatgaaata ttagctaaag
    15901 aaacagaaga agtcgttata aacgaattgg aacatgccca gaaattattg cggagaaagt
    15961 taaaataaaa aaatagttaa cattattcaa agtttttgtc cagtaattgt tgtaacaatt
    16021 actggttttt ttgtattttt tacatttttt taagag
//
LOCUS       DACDUU010000068        15852 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.068, whole genome shotgun sequence.
ACCESSION   DACDUU010000068 DACDUU010000000
VERSION     DACDUU010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.068"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            288..1427
                     /locus_tag="IWL30_002218"
     CDS             288..1427
                     /locus_tag="IWL30_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAP9706950.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1494..2069)
                     /locus_tag="IWL30_002219"
     CDS             complement(1494..2069)
                     /locus_tag="IWL30_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9706951.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2074..3048)
                     /locus_tag="IWL30_002220"
     CDS             complement(2074..3048)
                     /locus_tag="IWL30_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAP9706952.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPVVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3072..3116)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3331..4299
                     /locus_tag="IWL30_002221"
     CDS             3331..4299
                     /locus_tag="IWL30_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9706953.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4412..4690
                     /locus_tag="IWL30_002222"
     CDS             4412..4690
                     /locus_tag="IWL30_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP9706954.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4808..5044
                     /gene="secG"
                     /locus_tag="IWL30_002223"
     CDS             4808..5044
                     /gene="secG"
                     /locus_tag="IWL30_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAP9706955.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5156..5902
                     /locus_tag="IWL30_002224"
     CDS             5156..5902
                     /locus_tag="IWL30_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9706956.1"
                     /translation="MKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSLAEH
                     QLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5905..8262
                     /gene="rnr"
                     /locus_tag="IWL30_002225"
     CDS             5905..8262
                     /gene="rnr"
                     /locus_tag="IWL30_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAP9706957.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8284..8748
                     /gene="smpB"
                     /locus_tag="IWL30_002226"
     CDS             8284..8748
                     /gene="smpB"
                     /locus_tag="IWL30_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAP9706958.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9050..9769
                     /gene="atpB"
                     /locus_tag="IWL30_002227"
     CDS             9050..9769
                     /gene="atpB"
                     /locus_tag="IWL30_002227"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAP9706959.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9822..10037
                     /gene="atpE"
                     /locus_tag="IWL30_002228"
     CDS             9822..10037
                     /gene="atpE"
                     /locus_tag="IWL30_002228"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAP9706960.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10128..10652
                     /gene="atpF"
                     /locus_tag="IWL30_002229"
     CDS             10128..10652
                     /gene="atpF"
                     /locus_tag="IWL30_002229"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAP9706961.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10639..11181
                     /locus_tag="IWL30_002230"
     CDS             10639..11181
                     /locus_tag="IWL30_002230"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAP9706962.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11204..12760
                     /locus_tag="IWL30_002231"
     CDS             11204..12760
                     /locus_tag="IWL30_002231"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAP9706963.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12774..13676
                     /locus_tag="IWL30_002232"
     CDS             12774..13676
                     /locus_tag="IWL30_002232"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAP9706964.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13699..15105
                     /gene="atpD"
                     /locus_tag="IWL30_002233"
     CDS             13699..15105
                     /gene="atpD"
                     /locus_tag="IWL30_002233"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAP9706965.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15119..15541
                     /locus_tag="IWL30_002234"
     CDS             15119..15541
                     /locus_tag="IWL30_002234"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAP9706966.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         5246 a         2863 c         3430 g         4313 t
ORIGIN      
        1 tttgcaacta gcttctgtat atgatgttta caaagaatgg tacaagaaaa gatcatcctt
       61 gaaaaaggaa agctaacaat aactagcatc atataagagc ttttaaggat agaacagtag
      121 aataggaatc tatgggcaag tcctctcgta taaataaaat aggagggaag tagcagcata
      181 tttttcataa agtggctctc tttcttcatt tttactttgg ttgctcctat ttgaaaattc
      241 gagcacactc aaatacaata aaaagaaatg gcaagggaga ggattagatg aaaaaagaag
      301 aaaaaatcgc gattttacaa gagattatcc gaattaaatc agtgaatgga aacgaaggtg
      361 aagtagcagc ctatttgaat aagcttttgg caaggcatga cataacagga gagattgttt
      421 cttacagaga tggtagagat aatttgattg ctcgttacca aaagggccaa tcaggaaaag
      481 tgttaggcct atctggacat atggatgtcg tggcggctgg tgatgaatcg tcttggacct
      541 atgcaccctt tgcagcagaa atccatggga accgtttgta cggacgtggt gctacagata
      601 tgaagagtgg cttggcagca atggtgattg ccatgatcga actgaaagag tcaggaaaac
      661 catttaacgg aacagtcaaa ttattagcca ctgtcggaga agaagtgggt gaactaggtg
      721 gggaacagct cacgaaagca ggctatgtag atgacttaga tgccttgatc atcggagagc
      781 caaccaatta cagtttgatg tatacgcaca tgggatcaat caactataca gtcacctcgc
      841 acggaaaaga agcgcatagc tctatgccag accaaggtta taatgcaatc aatcacctaa
      901 atgaattcat tacgaaagca aatgctgaaa tgaatcacct cgctgaaaca atcgagaatc
      961 ctgtcctagg aaaaacaatc cataatgtta cattgatcag tggcgggaat caagtcaaca
     1021 gcatcccaag ccatgcgcag ctccaaggga acattcgatc gattccagaa tacccaaatg
     1081 ataaaatcat tgcattgctg caatcaatcg tcaatgaatt gaatcaagag acagactatc
     1141 atttggaatt gatgatcgat tacaacaaaa tacctgtcaa agctgatcca gattctcctt
     1201 tgattcattc cattcagcaa caattcagcc agccattacc tttagttgga gcggcagcaa
     1261 caacggatgc agccgaattt acgaaagcta accattcctt cgactttgtc gtctttgggc
     1321 ctggtgtcgt tactttgcct catcaagtag atgagtatgt tgaaatcgat aattatttgg
     1381 atatgatcga aaaatatcaa ggaattattt tatcctactt agcgtaagat ttttttaata
     1441 aatgaaaaaa cagagccaga ttagcattat tttgctaatc cagctctgtt tattcagcct
     1501 atttttcttt ttgaatatat caagcgttgg ggcaacatag tgatgactaa tgaaacaacg
     1561 acgatagaca acaagcgatc aaggtagtct gtacccacct gtaccaagat cacacttgtt
     1621 gtctgactta aacctaaccc atgtaatagc tgaacgatca tactggatcc tgaggaggtg
     1681 atcccgccaa ataactgaac ggtaatgatc gaagaaacga tcgttccagg taccgacaaa
     1741 atcaaagcga gtaataacag tttcaagctg tttttatctt tgccgagata tcttggcaaa
     1801 atcaatccag caagcaagcc tgtgagaatt tggattggcg aataatacag agaaaaaata
     1861 tctgtagtca ctccacttaa aataccgctg gacaatcctg tcagcattcc aggtacaggc
     1921 ccaagcaatg caccagcaaa aatcgttccg atagaatcga gatagattgg caaacgaagc
     1981 cataaagcaa tcgttcctcc aatataattg atcccaatac acaaagccat cactgtaagt
     2041 acgcgagttg ccattttgtt tttattcatt ttgttagccc atctttaacc tttccaaatc
     2101 tttcataatc gtttcttctt gtgcatttaa aagaacggtt aaaaactttc ggaaaaaaag
     2161 acgagtatct acttttgtca taattttact gttagcaggt ttctgccaaa attcataacg
     2221 atcaaccaat gtttgtccgc gacttattcc ttgagtttct acagcagtga agcttttaaa
     2281 gccttcacag atggactcat cgataaaata agcaacagct aatggatcat tgatgacaca
     2341 tcctaagatt ctttcttgtt tccagtgaaa gtcaaaataa aaacgagtga tcgcttttag
     2401 atactctccc tcttcaggat tcatttggca gcaatattca aggatagtcg gtgtcaagac
     2461 gatttctctt gtcacatcta acccaaccat ttctatcggt tgtttgagct tttcgaatac
     2521 ataagaagct gcttcaggat cacaccagta attgtattca gcaactggtg aacagttacc
     2581 atggcttttg taagttccgc ccattgagac aaaacgatcc atatgtttcc caacagatgg
     2641 gctgtttttc aaagcagaag cgatattggt caaaggcccc aaagcaatga ctgatgtatc
     2701 tgttttttct ttgaaataat cagtcagaaa atcaactgca tggagcgatt ctgcctgttt
     2761 ggctaagacc accgggaaat ttgtgtcccc taatccatcc aacccatgag tgtcttgcgc
     2821 actaatgaaa ggttgcttca aaggtttttc cattccttga taaacaggga tatctaaacg
     2881 atcacagcgc tccaaacatt tcagcacatt ctgcgtaccg atatctacag ggacattccc
     2941 gcaaacgacg gtaatagcaa ctacttcgat atcaggggat ttgacagcta gtgataaagc
     3001 taaagtgtcg tcgatccctg gatcgcaatc aataattact ttacgcatta tttccaatct
     3061 cctttcttta gagatactta gttttttatt ctgggaagtt tccgaaccag tccaagacga
     3121 gtgtcttgtt tataatgcac gaaattagta tactggcttt tattgtgaat gtaaatgctc
     3181 tcttacaagt aaatctattg agaagaactt taagtaaatg atacaatgaa aaagaaatgg
     3241 aatttacgtg tgccgaagaa aaagaatcct attgatcggt atattgtata ataaatagat
     3301 tatagtttgg cagaaagcgg cgggtgtacg atggttattt tttatttcat tcttattctg
     3361 atatttatag gaatcagtta ccattttctt tcttattttt atgcaaagaa attagtaaga
     3421 gcgggggtac agagaggcta tgactggtat gaagaaactg ggcaccgctt gatggatcct
     3481 gctgcagtca cgtcagtaga aaaaaagcga caaaaattag aagaagagtg tgaaagcttc
     3541 tgggaacaag gaataccgaa aacgatcaaa agttatgacg ggttgaaact agctggacag
     3601 atgttcctcc aaccaactca gcagaataaa tgggtcattt gtgtacatga ttatcgaagt
     3661 acagggaaaa gagatatgtc tcatataggt aaaagatatg cagagaaagg attcaacgtt
     3721 cttatacccg atttacgtgc gcatggagaa agtgaaggag aaatcatcgg aatgggatgg
     3781 ctagaccggc tagatttaat tgcatggatc caactcatct tagatgaaca accagatgca
     3841 tccattattt tgcacggcag ttcgatgggc gcttctacta ttatgatggc aagtggtgaa
     3901 aagcttccat ctgcagtcaa aggatttatc ttagacagcg gatatgtttc tgtttatgcg
     3961 gaatttcggt atatgttaag taaaatcacg gtctttccta aaaaaatgat catgagatat
     4021 gcgaatcact atgctcagaa atacgcaggt tattctttaa agcaagcatc agctacaaga
     4081 cagcttggta gtaaccattt gcctttgtta gtcatccatg gtgaaagaga tcattttgtt
     4141 cctacagaag cagcatacac gattcaaaac gctacggcag gggataaagc attactgctt
     4201 gttccagaag cagaacattt ggaagcatcg atgaaagatc cgaaaaccta ttggacagtg
     4261 attttttcat ttattgaaca aagaataacg atggattgat tttctcttcc tttttgtaag
     4321 caacacgagg ataaataatt gtttatagac catgagactg ttaaaataag gtaggaataa
     4381 aaaaatatca gttatttgag gagtgcggca tatggaaatc aaagaggaaa aaaatcgtct
     4441 tgctttattc aatgatgagc aacaagaaat cggagaaatg acatggtcgg atgcggggcc
     4501 agacatcatg atcattgacc atacatttgt cgatccagca tatcgtggtc aaaaattagc
     4561 tgaaaagcta gtgtatactg gagtagaact agcaagacga gaggggaaaa agattatccc
     4621 ttcatgccct tacgcaaaaa aagagtttga gaagaaacca gagtatcatg acgtcttacg
     4681 ccaatcataa taataacagc tgaaagattt gcactttcag ctgtttttcc atttatatag
     4741 agttgtcacg gagttttatc tatgataaag ttaaagagac gatatctttt gggaaggagg
     4801 ctgctatatg tataatttaa ttttgggaat cgtgatcgtt ttatcgattg ttatggtcat
     4861 tgcgattatg atgcagccaa gcaagcaaaa tagtgcagcg agtgcattta ccggcggtgc
     4921 ggatcaacta ttcggcaaac aaaaagctcg cggctttgaa gctgtcatgc agcgatcaac
     4981 tgcagtcatg ggagctgtct ggatgatctt attatttgtc ctagcatttt tatcttcgaa
     5041 gtagtcgaat aagcctctcg cacaacggcg ggcggctttt tttattgcaa agagaacgct
     5101 taagaagaag ttatgaaaga aactatggta aaatgaggcg aaagaggtga aacgagtgaa
     5161 acaattacca aaaccccttt atgcaaaaca tggaaaacga gcagttttgc tgcttcatgc
     5221 gtactcagga agtccaaatg atgtccggat gctcgcacgg tttttagaaa aagcagatta
     5281 tacagtctat gcgccgctat ttaaaggaca tggaacgatg gagccatatg atatcttgca
     5341 agaaaaagca gaaagctggt gggcagatac taaaaaagct atccattttt tacagtctga
     5401 acagttttct gatattgccg tattgggatt atcgatgggt ggcatctttg ctgtacgggc
     5461 actagaagaa gaatcagtaa ttggcggcgg ttttttctgt tcgcccctgt ctcctgtaga
     5521 aacaaacgtg cctgaaaatt ttgaaaaata tgtacgtcaa gtgctgaaga tagcaggcaa
     5581 atcagaaaaa gaaatcaatg aaaaagctgt gtcttaccgc tcgttagctg aacatcaatt
     5641 aatggatata caagaccaag cagcaatcgc agaaagtaga ctatcagata tccaacagcc
     5701 aatattttta gctcaagctg ggaaagatga aatgatcgac ccaaatggtg tatttgaaac
     5761 ggcacgcaaa ttgagccgcc aacgagtgac acttcaatgg tatcctgaaa gcggtcatgt
     5821 gattacagtt ggcacagcaa gacgcgaatt agaaaaagac gtgttggaat ttttagaaaa
     5881 attaccttgg aatgaggaat aaatatgaca aaagaaacaa taaaagagaa aattctcttt
     5941 ttcatggaaa gcagtcgcaa acgtagtttt tctatggaag aaatcgctga aggtctaggc
     6001 tttcaaaaaa gcgatgattt taaattacta gtacaaacga ttgctcaaat ggaaagagaa
     6061 cagagtatcg ttttcaataa aaaaggaaaa gtcaaacttc ctttaaaacc gatcttggtc
     6121 gaagggacat ttcgggcaaa tgaacgagga tttggatttg taacgattga tcccgaagaa
     6181 gatgatatct atatcccgaa agaagcgact ggctttgcaa tggatgggga taccgtagca
     6241 attgatatca tgaagcgagc aaatacagat atggatcgcg gagcagaggg aaaagtagtg
     6301 gagattcgag accgagcgac tactcagtta gccggagaat ttgttgctta ttcagacgaa
     6361 gagatacagg aaacggatct ttatggtgtg gtcatcccga aagatagaaa gcttaaccaa
     6421 ttcaaagtgt atgcagcggc agagggagtt cgtccagttg acggaagtat tgtattgatc
     6481 gaactgaccc attaccctga aaaagggtat gcaactagtt tagaagggat aatcaagcaa
     6541 gtgattggtc ataagaatga ccctggcatg gatatcctct cgattgtcgt ggctaacggt
     6601 attcctacaa aatttcctga tgatgtgtta tctgaggcag atgaagttcc agaccagatc
     6661 agcgaaaagg atattgttgg acgcagagat ttaagagatc agctcatcgt tacgattgat
     6721 ggagaagatg caaaagatct agatgatgca gtgacggtca aaaaattaga aaatggtaat
     6781 tattttcttg gagtacacat tgcagatgta tcttactatg tgacgcaagg cagtcaactg
     6841 gacagagaag catacgaaag aggaacaagt gtctatttga ccgatcgagt gattccgatg
     6901 atcccacaac gtttatccaa tggtatctgt tcattgaatc cccatgttcc acgactcacc
     6961 atgagttgtg aaatggagat cgatccaaat ggacaagttg tctctcatga gatttttcca
     7021 agtgtgatcc aaacaacaga acggatgacc tacacagctg tcaatcaaat cttagaagat
     7081 caagatgaag aaacgatgca acgttatgct cagctcgtgc ctatgttcca agaaatgggt
     7141 gaattgcatc gaatccttga aatgatgcga atcaaacgtg gagcgatttc ttttgaggat
     7201 cgtgaagcga agatccttgt tgatgctgaa ggacacccaa atgatatcca gctaagaagc
     7261 agagggatag gtgaacgctt gatcgaatcc tttatgcttg ctgccaacga aacggtagct
     7321 gaacattata ataaattaaa tcttccgttt atttatcgaa tccatgaaca gccaaaagaa
     7381 gaaaaaatgc agcgcttctt tgattttgct tcagctttgg gcattctggt ccgaggaacg
     7441 aaaggaacga tcacaccaaa agatttacag catgtgatcg aaggagtaga agacaaacca
     7501 gaagctgcag ttatcaatac tatgttgcta agaagcatgc aacaagcacg ctattcagaa
     7561 gacaattttg ggcattatgg cttagcggca gattactaca cacactttac atctcctatt
     7621 cgacgctatc ccgatctaat cgtccatcgt ctgatccgtt cttacagtga agataaaagt
     7681 gaaagcaatc aaagttcgtg ggctgcggaa cttcctgaaa ttgcagatca tagttctaag
     7741 atggaacgtc gtgcagtaga aacagaacgt gaagtagatt ccatgaagaa agcagaattc
     7801 atgatggaca aagtgggaga agaattcgat gggatcatca gctctgttgt gaaattcggc
     7861 ctgtttatcg aactgccgaa tacagtagaa ggattgatcc atatcaatga attgaaacag
     7921 gactacttcc attttgttga aaatcattta gctcttgtag gtgaaagaac tggtctgact
     7981 tttaaaatcg gacaaaaagt gcgcgtaaaa gtagtcaaag ctgatccaga tgaacgagca
     8041 gtggattttg aactcgtttc cgcagaagag gtcactgcgt tggaacgtcc aaaagcacgc
     8101 aatggaaaat ctggaaaacg agaaggaaaa caagcaggca aacgctcaga aaaaaatggc
     8161 aagaagaaca aacgatatag tgatagtaag caaacgttcg gtaagaaaaa agggaaaaag
     8221 ccattttaca aaggtgtcaa aaagaagaaa aagaaaaaat aaaatgagtt ggaggaaaca
     8281 gctatgccaa aaggcgaggg aaaactaatt gcacaaaaca agaaagctcg ccatgattat
     8341 tcgatcatcg acacgatgga agcagggatg gtcttgcaag gaacggagat caagtcgata
     8401 cgaaacagcc ggatcaatct gaaagatgga tttattcgcg tccgcaacgg ggaagctttc
     8461 ttgcataatg ttcatatcag tccttatgaa caaggaaata tttttaatca tgatccgttg
     8521 cgcacgagaa agttattatt acacaagaaa caaatcgttc gtcttgaaaa cgaattgaaa
     8581 aacactggaa tcactgttgt tcctttaaaa gtctatattc gtaacggcta tgccaaggta
     8641 ttgattggtc tggcaaaagg gaaaaaatcg tatgataaac gggaagattt gaaacgaaaa
     8701 gatatcgatc gacaaattga tcgaacatta aaaaatttct ctagataatt tcattatcct
     8761 ttttcgcata ttcggtgctt ttgcttttac ttaatgataa aatttgttag catttaatct
     8821 gataaaacat gattatcaca ggttttttct tagatcagga gcaccgtgca tggggaaaaa
     8881 ttcgcttcgt ttttgactga aataaaaaag tgtctaattt tcatgaaaat gttttgaatt
     8941 ttctgtttca tggtggtatg ataccgtgtg gttagaaacc gattggaaaa aaccagatac
     9001 gcacatgaat tgattttatg tgaatttcgg ggaaaagagg tgaaatggat tggatgaacg
     9061 ctcgctaacg tttcacattg gaccaatttg gtttgatggt acagtttgta taatggtgtt
     9121 actgacctgt ttgattgttt tcttcttagc ttactttttt acaagaaatc ttaagttgaa
     9181 accaacaggc aaacaaaatg cgatagaatg ggtcattgat tttagtcgag gaatcgtaac
     9241 agataatctg cctagaaaag aattgagcaa tttccatttg ttggcattta cattgttttt
     9301 atttgttttt gttgcaaaca acataggttt agtaacaaaa atcgtcttac ctaacgaaac
     9361 aacactttgg aaaagcccga cagcagaccc gtttgttact ttaactttgg catttataat
     9421 gatcttactt actcacttat tcggggtgaa aaagttaggc ttcaaaggat atttcgtaaa
     9481 ttcgttcttg aaaccctata gttttatgtt tccgatgaaa ctgattgaag aatttacaaa
     9541 cttattaaca cttgctttac gtctttatgg gaatatttat gcgggggaag ttttgctaac
     9601 cttgattgca aaaatgatga caagcttagg ctggttctca ttaccattag caattccatt
     9661 agagatggtc tggatcgcgt tctccttgtt tattgggagc atccaagcat ttgtctttgt
     9721 cactttgtca atggtttata tgagccataa gattgaagta gaagaataat actcggttaa
     9781 aaaacaaact actatttatc tattttagga ggaaaacaat tatgaattat atcgcagcag
     9841 caatcgcaat catgggagca gcaatcggag ccggttatgg taacggtcaa gttatctcta
     9901 aaacaatcga atcaatggct cgccaacctg aaatgtctgg tcaattccgt acaacaatgt
     9961 ttatcggggt agctctagtt gaagctgttc ctatcttagg ggtagttatc gccttgattc
    10021 tagtttttgc cgtttaattg aatcagtatt aaggcagaaa caacatcgtt tgttactgct
    10081 tttttacatg gaaactagaa atactccaaa gaaaggaagg ctcgcctatg ctgaatcaat
    10141 tggcaattgc agaagttggc aatgcgatgt taggtaatat catcgttgtc agcggctcat
    10201 ttttgatact actggctctc ttgaagcact ttgcttgggg accaatcagc gatattttga
    10261 aaaaacgtga agacaagatc gccaatgatt tagattctgc agaacaatct cgcatcaact
    10321 cagcgaaaat ggaacaagaa cgcgaacaac aattgttagc ctctcgttct gatgcagctg
    10381 atatcatcaa aaatgcgaaa gaaagtggag aattaagccg ccaaaatatt ttgaaggatg
    10441 ctcaagaaga agcagctcgt ctaaaaagca aagcccaagc tgatatcact gtagaacgtg
    10501 attcagcgct gaactctgta aaagacgacg ttgcagaact ctctcttcaa atcgcggaaa
    10561 aaatcttgaa caaagaatta tctccagaaa tgcatgaatc attaatcaat caatacattg
    10621 aaggtctagg ttcttcaaat gaaactagat aaatatacag taggaagacg ttacgggaaa
    10681 gctttatttg aactggcgat cgattcaaat caagcagaag aaatctatca agatctcgta
    10741 agtttacgtc aaatctatga acaagtacct ggattaggga atatgttaag tgatgttcgt
    10801 ctagaacctc atgaaaaacg agaaatcatg gacaagcttg tcagcggtta tgatggaatc
    10861 gttaaaaact ttttagaagt tgtttatgag tataaccgaa tggatgacct tttgttcatg
    10921 attgaagaat atgaacgtcg atatgatgaa catcaagggt tactattggg aagtgtcaca
    10981 acagcagtcc cactttcaga cgaacaacga atacaattag aaaagaatgt tgcaaaaaca
    11041 atggactatc aatcagttga attaaaacaa atcgttgatt cttctattat tggtggcgcg
    11101 attgttgaag ccaaccatcg agtaatcgac ggaagcattc gcacgcaatt agaaaaaatg
    11161 cgtaatcaat taaacagata gtacaaagag gtaggtgaat cgaatggcca tcaaagcaga
    11221 agaaattagt gccttgatta aagaacaaat tgaaaattat caaaacgtgc tttcagtcga
    11281 agaaatcggt accgtaactt atgtaggtga cgggatcgct cgtgcacatg gtttagaaaa
    11341 cgcgatgagt ggtgagttgc ttgaattttc aaacggctca tacggaatgg cgcaaaactt
    11401 agagtcaaat gatgtaggta tcattatcct tggcgatttc gagtccattc gtgaaggaga
    11461 taaagtaaaa cgaacaggta agatcatgga agttccagtt ggagaggcct tgatcggtcg
    11521 ggtagtcaat ccgctaggtc aaccaatcga cggactaggt gaaatcgtta ctgacaaagc
    11581 tcgtccagta gaagcgatgg caccaggtgt tatgcaacgt aaatctgtta acgaaccaat
    11641 gcaaacaggg ctaaaagcga tcgacgccct tgtgccaatc ggacgcggac aacgtgaatt
    11701 agtcatcggt gaccgtaaaa cagggaaaac ttctattgcg attgatacga tcatcaacca
    11761 aaaaggtcaa gatatgatct gtatctatgt agcaatcgga caaaaagatt ctacagttcg
    11821 tacacaagtt gaaacattga aaaaatatgg cgcaatggat tacacaatcg ttgtgaatgc
    11881 cggtgcgtct caaccagcac cattacttta tatcgcacca tatgctggta ctgcaatggg
    11941 tgaagaattc atgtacaacg gtaaacatgt attgatcatt tttgatgatc tttcaaaaca
    12001 agctgttgct tatcgtgaac tttccttgct acttcgccgt ccaccaggtc gtgaagctta
    12061 tcctggggat gttttctact tgcactcacg tttattagaa cgtgcagcaa aattaagtga
    12121 tgatctaggc ggcggttcaa tgactgcttt gccatttgtt gaaacacaag ctggagatat
    12181 ctctgcttat atcccaacaa acgttatctc aatcactgac ggacaaatct tcttagaaag
    12241 cgacttgttc tacgcgggta ctcgtccagc ggttgatgcc ggactatctg tctctcgtgt
    12301 agggggttct gcgcaaatca aagcaatgaa aaaagttgct gggactttac gtctagactt
    12361 agcaagttac cgtgaattag aagcatttac acaattcggt tctgacttgg atgcagcaac
    12421 acaagcaaaa ctaaatcgtg gacgccgtac agtagaaatc ttgaaacaaa aattgcatgc
    12481 accgctagct gttgaaaaac aagtagtgat tttgtatgct ttgacacatg gattcttaga
    12541 tagtatccct gtagacagta ttttagactt cgaacatgaa ttattcgaat atctggatac
    12601 caatcattca gatatctttg aaacaatccg tacaacaaaa gatcttccag aagaagaagc
    12661 gttgaacagt gcgattcagg aatataaaga catgttctta gctacaaaag gaaataattc
    12721 ctctacagaa gataaattga aatccattca aaatgcatag tgaggtgaga tgaatgggtg
    12781 cttcattaaa tgaaatcaag acacgtatcg cttcaacgaa aaaaacgagc caaatcacgc
    12841 gggcaatgca gatggtgtct gcttctaagt tgacaaaatc tgaagcttct tcacagaagt
    12901 ttcaggttta tgcaacaaaa gtaagagaaa tcgttacgca tttgactgct actcagttaa
    12961 atgatattgc ctcagaaaat cctagagggg atatcaatta caacagcatg ttgctcagcc
    13021 gtcctgttaa gaaaacgggt tatatcgtca tcactgcaga tggcggttta gtcggtggat
    13081 ataacagctc gatattgaaa caaacgatgt ctattttaga agaagaccac caatcacccg
    13141 atgattacgt gatgatcgca attggcggaa ctggtgcaga cttctttaaa gcacgcggta
    13201 tcaatctagc atatgagctg cgcaatctat ctgatcagcc aagctttgat gaagtccgca
    13261 aaatcgtaaa tatggcaaca actatgtacc aaaacgaagt attcgatgaa ttatacgtat
    13321 gttataacca tcatatcaat tcgctaacta gtcagtttcg tgtagaaaaa atgctgccaa
    13381 tctcagactt agatccagaa gaagcaacta ctttcgaaca agaatatatt tttgaacctt
    13441 caaaagaaga aatcttgtct cagttattgc ctcagtatgc tgaaagtttg atctacggtg
    13501 cgatcgttga cgccaaaaca gcagaacatg ctgctggtat gacagcaatg aaaacagcaa
    13561 ctgataatgc tgctaatatc attgatgagc tgacggtttc ttacaaccgt gcaagacaag
    13621 gggctattac ccaagaaatt accgaaattg ttgccggagc ttcggcacta gaatagtatt
    13681 gagattggag gaaaaaagat gagttcaggc aagattgttc aagtaatcgg tcccgttgtc
    13741 gacgtggaat tttctttaga ccaatcctta ccagatatca acaacgcatt agttgtttat
    13801 aaaaatgacg aaaataaatc aaaagttgtt cttgaagcag cgttagaatt aggggacgga
    13861 gtgatccgga ccattgcgat ggaatcaaca gatggtttac aaagaggaat ggaagtcatt
    13921 gatacaggca aagcaatctc tgttcctgta ggtaaagaaa cattaggtcg cgtattcaac
    13981 gtactaggag atacgatcga tttagaaaca cctttcccag aagatgcgga aagaagcgaa
    14041 attcataaaa aagcaccagc ctttgacgaa ttaagtacaa gtacagaaat tttggaaaca
    14101 gggatcaaag ttatcgattt gcttgcccca tatttaaaag gtgggaaagt cggactattc
    14161 ggtggtgccg gtgttggtaa aaccgtactg atccaagaac tgatccataa tatcgcccaa
    14221 gaacatggtg gtatttctgt atttaccggt gtaggtgaac gtactcgtga aggtaatgac
    14281 ttgtattatg aaatgaaaga ttcaggagtt atcgaaaaaa cagccatggt gttcggacaa
    14341 atgaacgaac caccaggtgc acgtatgcgt gttgctttga ctggtttgac gattgcggaa
    14401 tatttccgtg atgtagaagg tcaagatgta ctgttgttta tcgacaacat tttccgtttc
    14461 actcaagctg gatctgaagt atcagccttg ttaggacgga tgccttctgc ggttggttat
    14521 caaccaacat tggcaacaga aatgggtcaa ttgcaagaac gtatcacatc tacgaaaaaa
    14581 ggttcgatca catcgatcca agcaatctat gtgccagccg atgactacac tgacccggcg
    14641 ccggcaacag cattcgccca tttggatgcg acaaccaact tggaacgtaa attaacagaa
    14701 caagggattt atccagcggt agatccattg gcttcttctt caagtgcttt agcacctgaa
    14761 atcgtgggag aagaacatta taaagtagca acagaagtcc aacacgtatt gcaacgatat
    14821 cgtgaattac aagatatcat tgctatctta ggtatggatg aattatccga tcaagaaaaa
    14881 gtgttagttt ctcgtgcccg cagagtccaa ttcttcttgt cacaaaactt taacgtggct
    14941 gaacaattta ctggtcttcc aggttcatat gtccctgtag aagaaacagt gaaaggattc
    15001 cgcgaaatct tggaaggcaa atacgatgat cttcctgaag aagctttccg aagtgttggc
    15061 cgaatcgaag acgtcgtaga aaaagcgaaa acactaggct actagaaagg ggtgccccat
    15121 ggatcaatat ttaacagtaa atgtggtaac tcctgccggt ttggtctatg atcaccatgc
    15181 agctatcgtc gttgcacgta cgacagctgg tgagcttggg attttgccta aacatgcacc
    15241 aatcatcgtt ccattgacga ttgatgaagt tcgtgtaaaa cgaacagatt cagataccca
    15301 cgtcgattgg atcgctgtta atggcggaat catggaagtt cgtgataatg ttgtttcaat
    15361 cgttgctgat agtgcggaac gtgaacgtga tatcgatgta tctcgtgcgg aacgtgcaaa
    15421 acaacgcgca gaacgccaaa tcgaagaagc aaaagcaaaa caagatacga acgaattgaa
    15481 acgggcaact gttgcgttgc accgagcaat caatcgtatc aaagtatcta agcactcata
    15541 aaatgagatg cggatgttta tccgtgttta agaacagtaa aaagaggtta tgacagaagt
    15601 ggtcagcttc aagacataag aaggaattta cgaaaattgc ttctcaaatt tttgtgaaat
    15661 ctcagcttat ttttcgaaga agctacttct ggaacaccgt atatcggagg ttgtgaaatc
    15721 agcgttttgt cctgagtatt agagaaacaa atggaaaata gttcctcata ttttgccgtt
    15781 ttttgagcta ataccgaggg tcagatgatt gaacaccgtt tatttggaat aagggactgc
    15841 gacaagactt tt
//
LOCUS       DACDUU010000069        15274 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.069, whole genome shotgun sequence.
ACCESSION   DACDUU010000069 DACDUU010000000
VERSION     DACDUU010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.069"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(42..408)
                     /gene="ssrA"
                     /locus_tag="IWL30_002235"
     tmRNA           complement(42..408)
                     /gene="ssrA"
                     /locus_tag="IWL30_002235"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(579..1352)
                     /locus_tag="IWL30_002236"
     CDS             complement(579..1352)
                     /locus_tag="IWL30_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9706967.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1496..1846
                     /locus_tag="IWL30_002237"
     CDS             1496..1846
                     /locus_tag="IWL30_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP9706968.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1827..2543
                     /locus_tag="IWL30_002238"
     CDS             1827..2543
                     /locus_tag="IWL30_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9706969.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2543..3991
                     /locus_tag="IWL30_002239"
     CDS             2543..3991
                     /locus_tag="IWL30_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9706970.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4061..4570)
                     /locus_tag="IWL30_002240"
     CDS             complement(4061..4570)
                     /locus_tag="IWL30_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706971.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4560..5285)
                     /locus_tag="IWL30_002241"
     CDS             complement(4560..5285)
                     /locus_tag="IWL30_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9706972.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(5508..7628)
                     /locus_tag="IWL30_002242"
     CDS             complement(5508..7628)
                     /locus_tag="IWL30_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP9706973.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            7858..9036
                     /locus_tag="IWL30_002243"
     CDS             7858..9036
                     /locus_tag="IWL30_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAP9706974.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9052..9810
                     /gene="aroD"
                     /locus_tag="IWL30_002244"
     CDS             9052..9810
                     /gene="aroD"
                     /locus_tag="IWL30_002244"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAP9706975.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFFSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            9833..10318
                     /gene="ybaK"
                     /locus_tag="IWL30_002245"
     CDS             9833..10318
                     /gene="ybaK"
                     /locus_tag="IWL30_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAP9706976.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(10389..11642)
                     /locus_tag="IWL30_002246"
     CDS             complement(10389..11642)
                     /locus_tag="IWL30_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9706977.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            11759..13108
                     /gene="gdhA"
                     /locus_tag="IWL30_002247"
     CDS             11759..13108
                     /gene="gdhA"
                     /locus_tag="IWL30_002247"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAP9706978.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            13220..14566
                     /locus_tag="IWL30_002248"
     CDS             13220..14566
                     /locus_tag="IWL30_002248"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAP9706979.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(14874..15260)
                     /locus_tag="IWL30_002249"
     CDS             complement(14874..15260)
                     /locus_tag="IWL30_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9706980.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         4987 a         2737 c         3104 g         4446 t
ORIGIN      
        1 aataaggatt taagcatcta gcgaaatctg ctgaaatctc acaatggaga cggcgggagt
       61 cgaacccgcg tccaaacaca tcgccactta aaaatctacg ttcatagtca actcatttaa
      121 ggattcactt tacagcttgc cgagtgacag gcattctgta tcgctagtct gatgttctct
      181 ttttcaactt acagacggaa agttgaaacg tatcccacta aagttaggac ccttactcga
      241 gcacatgggc gatgccgaga ggatctacgc taactgtttt taggcagcta aagcgtaaga
      301 gttgttttcg tttttagcag ttatatttaa ctgtaacgtt tttacgtaga cgtaacctac
      361 gaaacgcaat tcaagctcga ctgtgcctgt cgaatccgta acgtccccgt tagctaacaa
      421 gggttacgtg tatttttcat acaaaattag tatagcatat attgggataa attcaataaa
      481 aaagcgaatc atatcccatt atttatataa taactgcccc tttcttaaga aaaaataagc
      541 gattttctta agaaaagagg tagcttcaaa aaaagatctt aaatcagatc aaaatacttc
      601 aatgcgtgga caattccgcc ttctgtattt ttctttgtga taaaggtaga aagttctttt
      661 aatccgtcat aggcgttgcc catagcgatt ttgttgtcgc aagcttccaa caaggcaaaa
      721 tcattcggac catcgccaaa tccaaaagta ggaacttttt ccagctgaag ttctttttgc
      781 agacgtttga cacctgcccc ttttgagatt cctttcttca ctgtatcgat cgagtaagga
      841 ccattccgat aaaaagttag ctcaggaaaa tgttcgtgat agtattcgtc acctgattga
      901 gataagatca gcaacatatt tactgtgttt tctttaaagc ctaaaggatc aatttccgga
      961 acatctgaat gaatgaagtc gtaagcattg atcattgttt ggctatgacc ggtacaccag
     1021 attttctgat cgttatagta agaaagttca tgaccgttca actgtacatg atcatacatt
     1081 ttttgacatt cttctgaaga aaatgtttga gaatagattt cttcgccgtc tactcgaatg
     1141 aaagaaccat tcatggtgac cgcagaagta atgcccgcat cttccatgat agagtggatt
     1201 tctgcttctg tacgtcctgt agcgatgacg gggagaacat tgttttcttt taatgcgttc
     1261 atagcttttg ccacttcggg tgtgattttt gatttcgcat ctagtaatgt tccatcaaga
     1321 tcaaagaaag tgattgcacg ataatttgac acaaataaaa ctcctttttt attggaataa
     1381 gtttatctta acaaagaatg aacaaaagac aaacctttcg tgaagaattc ctgaacatat
     1441 tggttcagtc aacaatttag agtatactat aagaagagaa tttaggaggg aaagaatgat
     1501 ttttttcagt attggtatct tattcttaat aattggcagt ctttttcgga ttctgccctc
     1561 aaaaggaaat ctgccttttt atgggtatca ttcgcctctt gccgcaaaaa cagacgctca
     1621 ttggcggctg gctcaaaaga caagcggaaa ctggtttttt cttatgggct tactgatggc
     1681 attgatcggc tattatttaa agacatctgg gcatacgaat tattttctga ttgaaatgct
     1741 tctgctagta tttccaatta tgcctatttt tattatgaca gaaaagaaat tacagaaata
     1801 tgacctggaa actggaggga acgataatga atattttaat gattgaagat aatcaatctg
     1861 tttctgaaat gatgcagatg tttttcttaa atgaaggttg ggaagcaact ttcaagtatg
     1921 atggaaaaga agggctcgat gcctttttag aaaacccaca acattgggat atgattacac
     1981 tagacctgaa tttgcccaca ctggacggta tgctagtagg aagagagatc cgcaaagttt
     2041 caagtactgt gccgattatc atgctgacag ctagagattc tgaaagcgat caggtgatcg
     2101 gcttagagat gggggcagat gattatgtca caaaaccttt cagtcctctg acgcttattg
     2161 ctcgaatgaa agctttgcat cgccgtagtg agttggcaga agcaagagaa gaagcagtag
     2221 tttcagatga tcattttgat atcgagacta gtcatttcaa gatgaatacg aaaacacgag
     2281 aagcgtatct ggacaaccgg ccaattgaag gactgacgcc taaagagttc gatctattgt
     2341 ttactttagc aaaaaaacca cgtcaagtat tctctcggga gcagttgctt gaattagtct
     2401 gggattatca gtactttggt gatgaacgga cagtggacgc ccatatcaag aaattacgac
     2461 aaaaaatcga aaaggtcgga ccacaagtca tccaaactgt ttggggagtc ggctacaaat
     2521 tcgatgattc tggtgttgcc taatgcgtta tttgtatcag caactcttag ctttctgcgg
     2581 aatggtcgcg ttgatcttat tgatcgttgg agtttctttt acacaactga caaaacaaac
     2641 gattgaagaa aacaattacc agcaactgtt tggatatgct gaatcagttg aaaagacaac
     2701 gcaaacctat gccgatacgc tcccgcagtt tagtcaagat caggcgtttc aaaatgcatt
     2761 gctgctgaca gagcaagtac tgacagaaca aaatgtgaac tttatattta tcgataaaaa
     2821 cgaacgtgtc atttatccga ctaatacagc tttactgaat tttaccttaa cagctgatca
     2881 atggaaaaat ctgaggaatg gacgtcaaga aaaattcaca tcgaataaaa atattttagg
     2941 acaagcacag gcgacctctt acgcacttgt gccatttaat ttgaatcatg aattctatgg
     3001 agcacttgta gttagtcaac ctgcgcgaaa tatcgataac agcgttcgtc caattacact
     3061 gaacctgttc aaaggattta ttttttccag tatcgttgca gttattgcca gctatgcttt
     3121 tgcagcattc caagttaagc gtatcaatcg gttaaggaat gcgacgaaag aagtgacgaa
     3181 tggtaatttt gatgtacagc tccccgtaca tgacaaagat gaatttgatg agttagcaga
     3241 tgatttcaat aaaatgacga attcattgaa agaatctcaa gcagaaattg aagaacaaga
     3301 aaatcgtcga agacaattta tggcagatgc ttctcacgag atgagaacac ctcttacgac
     3361 aatcaacgga cttctggaag ggttagaata caatgcaatc cctgaaaatc agcgagaaaa
     3421 cgcgattaaa ttgatgaaaa atgaaacaga gcgtctgatc cgtttggtga atgaaaactt
     3481 ggattatgaa aaaatccgta cgaatcaaat cagtatggtc attaaaaaat tcaatggaac
     3541 agaaacattg aaaaatattg ttgcacagtt agaagctaaa gcagaagcag caggagatac
     3601 attgacattg aaagccgttg atgatattga tgtttacgct gattatgacc gatttgtcca
     3661 aatcatggtc aatattattc aaaacgcgat acagtttact gaaaacggcc aaatcacgat
     3721 tactttagaa aaaggctatc ttgaaacgat catcacgatt gaagatacgg gaattggaat
     3781 gtctgaacaa caaatgaaaa gtatctggga tcgttattat aaagtagatc cttcaagaaa
     3841 aaatacaaaa tatggtgaat caggattagg tttaccaatc gtgcagcaac ttgtacgctt
     3901 gcataaagga aaattagaag tagaaagcga actaggaaaa ggaacaaaat tcgctgttcg
     3961 tttgcctgac gtagaaatca aaaaagagta aaaaacaagc ggtataaaag atcatgaatc
     4021 gatcttctat accgcttgtt tttactataa aatgattgat ttagcccgcg ggctctaaac
     4081 gatcaatata aggactttga gcaatagtag catcatgagt aaagagtaag ataccttgat
     4141 tttcttcatg agctaaataa gaaagcaagt ggagtagctc ttggatctcc aatacactaa
     4201 gttcatcaaa tgtatcttca ataacaatat tgtttcgctt ggaaagcaag gcatgaagta
     4261 attgcagctt tactttttca aaagacgtca actcagctgc cgcacaatta agcaaggaag
     4321 gagagatatc aagtttttct tgataggcca aacgatcttg tttgatttct ttttccggta
     4381 catccaacag gacatggtct ttaagattca gataaggaaa catcggccaa tttttttgga
     4441 tgatcgcaaa attatccagt tttgcaacaa gaacctcttt tttcaagtgc tccaatattt
     4501 tatgctgttt ttcgagagga cacaaaataa cggtcaagtg ttcagcatga atcaaatatt
     4561 caggtttcat tacgtccagg cctcctactt tgcaaatgat ggataaaaaa agtgaaaggg
     4621 aatagactaa gaatgagaca gcatccactg agcccaataa gagtttgggc aaatccctta
     4681 agtatatctg tgaaaaaaag atcattttgg aaatcgatgg taaaaaaagt ctgttgatcc
     4741 atcggaattg taagtccgct taccgaagtc aagatatcac ggatatctgg cagttcagta
     4801 gtctttaaga caagaaaatt gatcctttga agaagcgctt cataaactga ctggaagctt
     4861 acgatccaca ataaaaccaa gatacaacaa agaaagagag gaaagaacag ctgccagaaa
     4921 ataaaaagga ataatttggt tttagaaaga ccagtcaggt gccaattgat caattcctgc
     4981 ttgttttgcc tgactgaata gtaaaagaaa aagataaaaa ggaaggcaaa tagaaaaaat
     5041 aaactgaata tgccttttat ataaaatcgg ctagctaatt ctaaagccgg aagaagctct
     5101 gtagatgcag aagcgccaat aaatgaaagc cgatcttgga cttgattggc taaagccgat
     5161 tgcaaatggt aaagagtcag gcatgctatc aataaaaaag cagaaaagct tgaaaataaa
     5221 caatagagta ttgtctgctt tttgtaataa attaatcgag cagtagcata gcggattatt
     5281 ttcatacttt tcacctctat aaaaagtata gaaaatgaat atgaataaag tatgaaattc
     5341 ctttagtgaa atcacgaatt aaaaccagaa aatattttgt aaaaagcaag aggttgtgaa
     5401 gaagctgccc tattctatta ccgtagatgc aagcttttga acactgttta ttcgaaaaga
     5461 ggagcagcga caataatttt gtcgctgctc cttttaaatt tattttatca ttcggaagaa
     5521 cttgtggtcg ttccgtgaag ttcttggtac gtttgcgttt tataaagatc aacagttgat
     5581 tgtccgttgt gttcactaaa gacactagtt gacttgtcac caagagattt ttcagcttct
     5641 tttaatttct ctaagccatt cttgtagtca taatcactgg tatcaattgg gtctaatcca
     5701 ctattcgtat agaaccgaag aagatcacca ttattgattt ggtcagaaac agctaactgt
     5761 ttatcaactt tttctttcag tgaatcaatt tcattctgta cttcctcagt aggttcagta
     5821 atagcttcac cagtttcgtt actatagatc gtaccaccgt agtaagtata ttttggtgta
     5881 acaaaatcac catcacggaa agaaacaagc tgttcatgat ctttcgagaa tagatcttgt
     5941 cctaactgaa tataattttt tgtatctaca cctagtaagt gaagtaatgt aggcaaggca
     6001 tcaacttgtc cgccgtaagt gtgattgatt ccgccctgtg tttgtccagg gatatggatc
     6061 atataaggga cacgctgcat ttgtgcattg tcgtagtttg tccaagtaga acttgttttt
     6121 ccaactaatt cagctagatt tttatttctt gaattcgaga caccgtaatg gtcaccgtaa
     6181 aggacgatga ctgaattatc atacaagccg ctttctttca aataattgaa gaattcttgt
     6241 acggcggtat ctaaataatt ggctgtcgca aagtaaccgt tgatcgtttc atcatttgta
     6301 ttggctaacg ggaatcctgc ttcgtcattg gtgaattttg aataaggata atggttagat
     6361 acagcaatga attttgaata aaatggttgt tgcagacgtt ccaagtattg tacggattgt
     6421 tggaaaaatg gtttatcatg caacccgtat tggaacgaat tgtcatcatt aacatcataa
     6481 taagaagaat caaagaaata atcatatcct aaattcttgt atgtctcgtt tcgattccag
     6541 aatgtaccag cgttcccatg gaataccgca ctaatgtaac cttgtgtttg acttaaaata
     6601 tcaggtgcag cttggaaagt attttttccg cctaattgtg tgaacaagga gccttgattc
     6661 aagccaaaca atgaattttc catcagcgtt tccgcatcgc ttgtttttcc tgcttttact
     6721 tgatggaaaa agttgtcaaa actgaacgtg ctgttgctgt ggtaaagact attgatgaaa
     6781 ggtgtaactt catgctggag accattctca tctgtcaatt tataatcgat caggaattgc
     6841 tggaagcttt ctaaatggat ataaatcaca ttttttcctc tagcgagccc gaacaaatcg
     6901 tcatttggtg ctgcataatg ttcttttaca taattttcta cttctttcat atcattggga
     6961 cttgcttctg cccgcacttg gtttgtctgg taagtttgaa caccgtcata aaccatgaaa
     7021 gcattcaagc caagatattt gaccaaataa tcacgagaga atgttcgagt cagcaattct
     7081 ggacgatcag cttccgccat aaaaagattt cctgagaaaa tcataactgc taatgtagag
     7141 atcgcaaaag ccattcttgc tcggatcggg cgagggtcca acttgatttt ttttgcaatc
     7201 agcagaacag tcaagataat aaaatcgatc caatagatca catcatatgg gcgaaacagt
     7261 cgaatggcac tttctcccag accactcgct actttcccag cacctaaaat cgtgttgatc
     7321 gtaatgaagt ccgtgaattc tcgataataa gtgacattcg aaaaaacaag caatgtcaaa
     7381 agaaaataaa tgaccatcat cgtgatataa gcaactctag ggcgtctgat ataaagagca
     7441 attccaagta ataacaatgt agttgcaata gggttgataa acaagataaa gtattgaatg
     7501 ccactttcga ttccgagatg gaaatcaact aaataggcaa aaatcgtttt caaccaaagt
     7561 aaaacgacta gtaaagaaaa aaatccgagc ctcttattca aaaaggcagg tgtttttata
     7621 catttcaaga taatcttcct ttctgcactt cttaatcaca gaagtgtcct gaaaccatgt
     7681 tacacagctc acttattctc atttatttta ctagtatgac aatatagtgt caattgaaac
     7741 cagaagttta tgctgtttca ttaaggtttt ctttaagctt catgaaaaaa tgagcagtaa
     7801 gacaagtcct atgttttttg ttatacttgg gaagagaaag ctaaagcagg tgaaggaatg
     7861 aaagtaaaag taaaaaaaag agcaagcaat cgattgaaaa aaggatatcc actgatccaa
     7921 aaagaagacc ttgctcatcc ccaaaaagaa accacgcaat gggtggaatt agttgattcg
     7981 caaaaccaat ttatcgcaaa aggatatcta ggaaagcaga ataagggaat cggttgggta
     8041 ttgagccagc agaacatccc atttgatcaa tctttttttg aagaattttt tgtgaagtca
     8101 aaagaaaaaa gaactttctt tttttcagat gattctacta ccgcttttcg tatattcaat
     8161 ggagaaggag acgggatcgg cggtctgatt gttgattatt atgcaggtta tgctgttttt
     8221 tcttggtata acgaaacgtt atatgcacat cgtgaattat tgattcaagc ttttcgtgaa
     8281 atctacccgg aaatcatagg aggctatgag aaaattcgat ttgaatcagt aaatctgcct
     8341 gagagccagt tcatttacgg aaaagaggca ccggaaccct tgatcgtaaa agaaaacgga
     8401 gtaaaatttg ccgtttattt aaatgaagga ttaatgactg ggatcttttt agaccaaaaa
     8461 gaagtacggg gatcattagc agaaggtttc gcttctggga aaacagtttt gaacatgttt
     8521 agttataccg gcgccttttc tgttgctgcc gcaatgggag gggcaatcga aacaacaagt
     8581 gtcgatctag caaaacgcag tttaaaaaaa acacaggaac agtttgaagt gaatggtttg
     8641 gatgtcgatc agcaaaaaat cattgttatg gacgtattcg agtatttcaa atacgccaaa
     8701 agaaaacaat ttttatatga catgattgta ttggatccac caagttttgc cagaaataag
     8761 aaaaaagtct ttcgcgtagc aaaagattat ggggaactgg tcaaggatag tctggatatt
     8821 ttagcagaag acgggatact tattgcgtct acaaatgctg cgaatgtctc gatagaacaa
     8881 ttccaaacaa tgattgaaga ggaattccat gccaaaaatg tttcttttga acaacttgct
     8941 ttatatcgtc tgccaaaaga tttccagacg acggatacct ttttagaagg caattatctg
     9001 aaagtgtttg tttatcaaat taaaaataac cattagaagg agcgatgcag aatggttcgg
     9061 gtaaagggat tgaatatagg aagcggtaga ccaaagattt gtgcgcctct tatcggggcc
     9121 gtccgagagg aagtattacg tgaagcagtt cttgcacgtg aagcaggggc cgatttagtc
     9181 gaatggcgag tcgatcatta tcgtgaagtt ttttcttttg atgaagtagc agaaacattg
     9241 gttcttatcc atgagatgct tgatggactg ccgttattat ttacgtttcg taccttagct
     9301 gaaggcggag aacaagacat atcgatccgt cagtatcgcg acctttatga acatgtgata
     9361 cacacaggat tagtagatat ggtcgatatc gagttgttca aagtagaaag tctaggaaaa
     9421 agactgatag aggaaatcaa gtctttaaac attcctttaa ttatcagtag tcatgatttt
     9481 aaagaaacac cagcagaccc tgtcttgctt taccgattga atgttatgga acattttgga
     9541 gccgatatcg gaaaactggc agtaactccc aataatgaac gagatgtttt gcggttgatg
     9601 gagttgacta gacgggcaaa tgcttttttc tccatgccta ttattacgat gtccatggga
     9661 aatcttggta aaatttctcg tctggttggc ggaacgacgg gttccgttat gacatttggt
     9721 tccttaagca gcgattctac ttctgctcca ggacaattac cggtcaaagc attgaagcaa
     9781 attattcaat tattaaatga accgttgtag aacaagtttg aggtgatatt ttttggcaaa
     9841 gaaaaaaata gtaaaaacga atgcaatccg tatggtcgaa caaaaaaaga ttccctatac
     9901 agagcacgaa tatgagtggg acgaaagcca cctaagtgcg tcatcggtcg ctgaacagct
     9961 tccagaaagc caatctcgta tatttaaaac actggttgct gtgggcaatg tgacggggcc
    10021 tcttgttgct gtgataccgg gagaagccga attgaatcta aaaaaattag caaaagtttc
    10081 agggaataaa aaagtagaaa tgctccattt aaaagatttg gaagcaacga ccggatacat
    10141 acgtggtggc tgctcgccta taggtatgaa aaaactattt cctacttatt tagatcaaat
    10201 tgcagaaagc tatgaacaaa tcattgtctc ggctggcaga agagggctac agatggagtt
    10261 agcacctcaa gatatctgtg cattaacgag cggacaattt gcagatataa aacaataaat
    10321 catacgtata aaagaaagaa ggaggccggg atgttgtttg tccagtctcc ttctttcttt
    10381 tttatacatt atgctctttg cccggcattc attcctgcta catgccctgt agcgaaagcc
    10441 gctgtgatat taaagccgcc agtataacca ttgacatcca gtaattctcc cgcaaaatac
    10501 agaccatcaa tgattttact ctccaatgtt ttaggataaa tttcctttag agaaacccct
    10561 ccgccagtta caaatgattt ctcaatagga aaagttttgt tgattgatag cttaaactct
    10621 ttgcatagtt gaacaaaatc ttgaatttgt ttttgagagg cttgttgtcc tgtcagatga
    10681 tccatttcaa gccgctcaag aaagaaaact aacaatcgct caggcaatat accatgccaa
    10741 gcattgacca agttcttttt ggttgcttta cttttttctg tcaatatatg aataagctct
    10801 tgtttggttt gtgtagggaa acaatccaga gaaagtgtca ctggttctcc cgttttctct
    10861 aattctttat tgacaaaact tgaacaacgc agtgcagccg gaccagaaat accgaaatgg
    10921 gtgaaaagta aatccatcgt atgttcagtc aatacacgac cttttttatt taaaacgcgt
    10981 aaagtgatgt cttgtaaaga aagcccttga agtgttttct cctgaatata cggttcatcc
    11041 gatatcaagg gggactctgt cgcataaaga ggcgtcaccg tgtgacccac tcgcttcacg
    11101 atcttgtacc catctccagt agaaccagta gatggataag tccgtccacc agttgttaag
    11161 ataatggcag gagcatggaa ctcttcgaat tccgtgcgta taccgtaaat tttatgttct
    11221 ttgtggatca atttctctac tcgagtagag aatagaagcg tcacatctag ttctttcaga
    11281 cgattgagca atgcttcgat aatcgttttt gatttatttg ttacaggaaa catccgtcca
    11341 tgatcctctt cttttaaatg tacgccttgt gattcaaaga agttcatgat atcgaaatta
    11401 ttccattgcg caaacgtact atataaaaac tttccatttc caggaatatg tgcaatcaaa
    11461 tcatcaacag gacgattgtt cgtaacattg caccgaccac cacctgtcat cagtagtttt
    11521 tttcctgctt ttttgttttt ttcgatcaag agtacgcgtg cgccttgttc agctgcactg
    11581 atcgctgcca tcatcccgct tgttccagcg ccgactacga tcacatcata cagtttttcc
    11641 attccctcac ctcaaggaaa gtgtagcata tctgaagaaa gaaaacagaa aaaaatgttt
    11701 tgagccaaaa gagaaaatga gctaaaatga gggaagataa gcaaaggagc gtggaacgat
    11761 gacaaacgca acagaatatg ttcaagcaat tcaagaaaag ctacatcaaa aggatcaagg
    11821 gcaagaagaa tttttacaag caatcgatga attcatgccc acggttatgt cttttttgga
    11881 cactcatccc gaatatatcg aaaagaatat cttagaactt ttaacggaac cagaaagagt
    11941 tattcaattt cgagtgcctt ggcaagatga tgaaggaaac tggcgagtaa atagagggta
    12001 ccgtatccaa tacaactcag ctatcggccc ttataaaggt ggattgcgct tccatcctag
    12061 tgtcaatcta agtatcttga aatttttggc ttttgaacag attttcaaga atagtttaac
    12121 cggtctgcca atcggtggag gaaaaggcgg aagtgacttc gatcctaaag gaaaatctga
    12181 taatgagatc atgcgttttt gtcaaagttt catgctggaa ttgtcaaaac atatcggccc
    12241 atcgattgat gttcctgcgg gagatatcgg tgtaggagct agagaaatcg gctatttgtt
    12301 cggcgaatat aaacgattga aaaaatatga tactggtgtg ttgacaggta aacctttgga
    12361 tttttgggga agtaagatac gtacggaagc tactggttat ggattagtgt attatgtcaa
    12421 acatctattg aacgaagaaa aagattcatt taatcaaaaa acagtttttg tttccggaag
    12481 cgggaatgta gccatttatg caatcgaaaa agtgcaagag ctaggcggga aagttgttac
    12541 atgttcagat tctagcgggt acatctatga tccagaagga atagatgtga aactattgaa
    12601 agaagtcaaa gaaatcaata gacaacgttt gacggaatac gcaaaccaac gtccttctgc
    12661 tatttatcat gctggagaat ctgtttggac attgaaagaa aaagcggata tcgctttgcc
    12721 atgtgctact caaaatgaaa tagacgaaag cttggccgaa atcttagtag aaaatgggat
    12781 caaaatcgta gcagaaggcg ctaatatgcc atgtacagta aaagctgttt ctgttcttca
    12841 tgaagcaggc gtgtggtatt gtccaggaaa agctgctaat gctggaggag tagcagtatc
    12901 tgctttagaa atgagtcaga acgtccagcg attgacttgg gaaagagagc aagtggatga
    12961 gcagctggat caaatcatgg aaacgattta ccatacgtgt cgccaaacag cagccgagta
    13021 cggcgacgaa aaaaatctgc ttcttggagc aaatgttgct ggatttgaaa aagttgccaa
    13081 agcaatggca gcagaaggtt tagtttgaaa aatgaaactt tcttggaaat attgtcagca
    13141 ttttgtttat ttgcttgaaa aataataaaa aaaaacgtaa agtattacct gtagagtaaa
    13201 cctataagga ggagaacaaa tggcacacat tcattttgac tattcaaaag tagcaccgtt
    13261 tgtcaacgaa caagagttag ggtacatgca aagccaagta acagcagccc acaacgaatt
    13321 acgggaaggt actggcgcag gaagcgactt ccgcggctgg atcgatttac caacgaacta
    13381 tgacaaagaa gaatttgcac gtatcaaaga agcagctaaa aaaatccaat ctgattctga
    13441 agtattagtt gttatcggta tcggtggttc ttatttaggc gctcgtgcag cgatcgactt
    13501 cttgaatcac acattcttca atttattaga tagcaacaca agaaaagcac cacaaatctt
    13561 ctttgccgga aattcaatca gctcaacata tattgctgac ttgatcgaag taatcggtga
    13621 tcgcgacttt tcagtaaacg ttatttcaaa atctggaaca actactgaac cagcgattgc
    13681 gttccgtgta ttcaaagagc ttcttgttaa gaaatatgga caagaagaag caaacaaacg
    13741 tatctacgct acaacagata aagcaaaagg tgcagttaaa gtagaagcag atgcagaagg
    13801 ttgggaaact ttcgtgattc ctgatgacat cggcggacgt ttctctgtat taactgctgt
    13861 tggtttgtta ccaatcgctg ttagtggtgc agatatcgat gcattgatgc aaggtgccgc
    13921 tgatgcaagc aaagcatact caagcgacaa actagaagaa aacgaagctt accaatacgc
    13981 agcaatgcgc aacattcttt accgtaaagg caaagtaact gagttgttga tcaactatga
    14041 accaggaatg caatacttct cagaatggtg gaaacaattg tatggtgaat ctgaaggaaa
    14101 agaccaaaaa ggcatctatc cttcaagcgc aaacttctca actgatttgc actctttagg
    14161 acaattcatc caagaaggac gtcgcaatat ttttgaaaca gttgtgaagg ttgaaaaacc
    14221 tcgtaaatca atcacaattc ctgaacaagc agaagattta gacggtctag gctacttgca
    14281 aggaaaagaa gtggacttcg taaatacaaa agcatttgaa ggaacattgc ttgctcatac
    14341 agacggtgac gtgcctaact tgctagtcaa aattccagaa atggatgctt atacattggg
    14401 ttatacaatg tacttctttg aaattgctgt aggtatttca ggttacttga atggaatcaa
    14461 cccattcgac caaccaggtg tagaagctta caagaaaaac atgttcgcac ttctaggcaa
    14521 accaggcttt gaagatcttg ctaaagaatt gaacgaacgc ctgtaagata agggtttttc
    14581 gctagattta aataatcagt gatagaaaca tgttcgcttt aaaatgaaaa ctggtaccca
    14641 tttaggtact caaacatact gcatcgttta ttcagaataa ggggctgtga caagacgttt
    14701 gtcacagtcc cttgtttgta tagaaatcga ttgattgcct tgctctttag tttgttttag
    14761 gactttaaga acagtgaggc tgacttcgaa ggttaattct gtttttttgt tttctagaat
    14821 cagtaagcaa aaaagcactc acttcgagtg cttttttgcg atatattctt agcttaagat
    14881 aggcctaatt tttcttttac agatgagggt accttatcgc tggtaattac ttttttctct
    14941 atgatccgct tttcgcttgc tttcaactgc aagatcgttc cttcggggta agcagaacca
    15001 ttggcagagt caccagaagt aatgacatcg aaatctaact gtcttttttc accgttttca
    15061 gcataagcat ctacgttgta tttaaaacct cttccaatta cttccccatt atctgctttg
    15121 atatcagttt ctgcaggcat tggagctttg actaccgcat aataatcttt acctacataa
    15181 gtagactgat agtagtccca acctttccat cctataaaag caatcaatat gataacgatc
    15241 aaccctaaaa tttttttcat gaaagatctc cttt
//
LOCUS       DACDUU010000070        15165 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.070, whole genome shotgun sequence.
ACCESSION   DACDUU010000070 DACDUU010000000
VERSION     DACDUU010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.070"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            278..787
                     /locus_tag="IWL30_002250"
     CDS             278..787
                     /locus_tag="IWL30_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9706981.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="IWL30_002251"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="IWL30_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9706982.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLTPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="IWL30_002252"
     CDS             2322..3302
                     /locus_tag="IWL30_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9706983.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="IWL30_002253"
     CDS             3424..4842
                     /locus_tag="IWL30_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAP9706984.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="IWL30_002254"
     CDS             4913..6382
                     /locus_tag="IWL30_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9706985.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="IWL30_002255"
     CDS             6392..6859
                     /locus_tag="IWL30_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP9706986.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="IWL30_002256"
     CDS             6895..7767
                     /locus_tag="IWL30_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9706987.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7849..10383
                     /locus_tag="IWL30_002257"
     CDS             7849..10383
                     /locus_tag="IWL30_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9706988.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10539..11312
                     /locus_tag="IWL30_002258"
     CDS             10539..11312
                     /locus_tag="IWL30_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9706989.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11305..12096
                     /locus_tag="IWL30_002259"
     CDS             11305..12096
                     /locus_tag="IWL30_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9706990.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12106..12597
                     /locus_tag="IWL30_002260"
     CDS             12106..12597
                     /locus_tag="IWL30_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9706991.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12615..13025
                     /locus_tag="IWL30_002261"
     CDS             12615..13025
                     /locus_tag="IWL30_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9706992.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13029..14429
                     /locus_tag="IWL30_002262"
     CDS             13029..14429
                     /locus_tag="IWL30_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAP9706993.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14452..14826
                     /locus_tag="IWL30_002263"
     CDS             14452..14826
                     /locus_tag="IWL30_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP9706994.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5270 a         2128 c         3037 g         4730 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga cccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttt caattagtaa ttgaagggac gcctaaaagt gaaaaaaaaa
     7861 atgaaagatg aagtattaga gtatatcaaa aataatttaa agtattacga tttttctgct
     7921 caagatattg caatgaaatt ttgtattaaa agaaatgtcg cttcccacta tttgaatcag
     7981 ttattttcag atggaaaatt actaaagaat gattctgtta gaccagtaat gttcaagtat
     8041 aatcaacaaa agccaaaaga ctgcttttct aagtttattg gagcagacat atctttgaaa
     8101 tccactatag ataaatgcaa agctactgta atgtatcccc ctaatggcct cccattaata
     8161 ataaaaggaa acagtggcgt tggaaaaagt tttttagcaa gtttgatcta tcagtatgct
     8221 cttgatagaa aagtaattca taatgacgca aaatttgttg ttgtgaattg tgcagattat
     8281 gcaaataatc cagagctatt gtcagcagtt ttatttggat ataaaaaagg agcctttact
     8341 ggggctgaaa aagatactgc agggatgcta tcttctgcta atggaggata ccttttttta
     8401 gatgaggttc acaatctaag tgcagaaaac caagagaaat tgtttcttct tatggattct
     8461 caaaagtatc gtaaacttgg tgaaagtgtg caatgggagt atgcaaatgt tcgccttatc
     8521 ttagccacaa cagaagataa aaatacctct ttgttagcaa cttttagaag acgcatccca
     8581 gctgaaatca ctctgccaga ttacaataat agaagtacat ctgaaaaaat tcaattatta
     8641 tttgaatttc ttaaagatga agcaattaca attgattcaa aaatattttg ttcgattgaa
     8701 ctttttactg atcttttatc gaaaacattt gaaggaaatg ttggagaatt gaggaatgaa
     8761 atcaagcttc tttgtgcaga aggttattta ggaaataaga gaaagtcttc aatttattta
     8821 ggggacaaac tagacagcgg tttttggata gatccagaaa ttagtataga tttaaaaaaa
     8881 atgtttctta aatcactttc tgaaattgat ttgattgaac tttttcaaaa tcacatttca
     8941 attgaagaaa gcatgagtca attaagagat agaatactta aagagtgtgc aggttcttct
     9001 gagtataatt atttagaaag tgacgaattt atagcattaa gaaactatgt tgttaataaa
     9061 atatcaccaa ttattgattc gacaggtcta tgtttattag atgaattctt ggcggatatc
     9121 agtctattta taatgtttat tgatgtaatt gaagaaacat caagaaattt tttgctgtct
     9181 aagttcagaa aatttcgtat gaaaaatgat aaacaaaaat tattggcaaa tgagatttgg
     9241 agtagtttag aagtcgaaga aagtaaacaa gaacttttgt tgaaatggat actttttctt
     9301 gtaaagaagt tctatgtgaa aataccagaa acccattgtc tgatagttat gcacggtaaa
     9361 ataacagctt ctgcgatcgc aagggaaaca aataagttgc tgaatacata tgtttatgag
     9421 gctttcgata tgccaattga aggtgagact agtgacttaa ttaacctaat caacaatttt
     9481 tgtaaaagta tagatacgac gaatggactc attctgctag ttgatatggg atcattggaa
     9541 caaatgtatg aaaaaattga atccaatgta ataggtgatt tggtaatttt aaacaatgtt
     9601 tctacagctt tagcgattga atgtgggatt caagtttgcc aaaaaaagcc aattagtcat
     9661 ttttaccaga tggattttga ttcgtttcaa gttaaagttc agtattataa aggattatca
     9721 caaaagaaaa atgtaatagt atcttgtctc tctggtgagg ggatttctga aaaagttaag
     9781 gatattttaa aacggtatct cgataatcaa gtggagattt tgatttttga ttttaacgca
     9841 ttaaagaaaa tagcaaagga aaaagatact atgattttta agaatacaat ttgtgttctt
     9901 tcaaccactg aattttacat tcaaggaatc gattgtttaa atttagaaaa tctaattaat
     9961 gggaatcaaa ccttagaaaa attaaataag tatatggact cggatagctg tgagttctgc
    10021 ttaaatgaat tagttaaact ctttactatt gaaggcgcta gtactaagct tcgcttttta
    10081 gattccaaaa aagtattcaa tgagatagaa aaagtactat atttgtatga aaaatattac
    10141 gatgtcaaaa tcccaagttt tctgagaatt aatctatttc tacatctttc tggaatgatt
    10201 gaacgaatta tgattggtga tggaataaca aatgaacatt tgaaagaggg agcgcaacta
    10261 tttgatactt ttttaaatgt atcaaaagat tttttttcag aaatcaaaga tatttactgt
    10321 attgaaatac ccaaaggaga gtatgagtta atctatacaa tttttgagca gactattttc
    10381 taaatagcat actagcgtct aaaaaaatga aagggtgtcc aaagtatatt tagacgctca
    10441 tttaattaat atttactgta ttttcccgct ttattggata atgttagttg gcacagtaat
    10501 tgcttatata aaagtataag aagaaaggag agaacaaaat ggcgttttgg caaacatttt
    10561 tagttgtgtt gtatggattt ttcattaact gggataagga agggcccaaa atcggaacct
    10621 cccaacctgt cgttgcaggt ttcttgaccg gacttattat gggagatatt caaactggac
    10681 tttatatcgg tgggactttg caattaatga cactaggtat tactagtttt ggtggtgcat
    10741 cagtaccgga ttatcaaaca gcagcattga tcggtactta tttagcaatc tcaactaacc
    10801 aggatgcttc tataggaata actttagcaa taccggtagc aatgttaatt gtacaactag
    10861 atgttctaaa gtggtcaaca aacatttttt ttcaacaaaa ggcagaaaaa ttcgcacaaa
    10921 aagaagatta tcaaaaatta gagtggatgc aatatttggc atcaattaat acaagctttg
    10981 tatctggaat tcctgttttg ttaactgtga tattaggccc aacgttagtg ggaagtgcaa
    11041 tcaattatat tcctgaatgg gttaccggtg gtttaaaggt agcaggggga ctattacctg
    11101 ctgttgggat aggaatgcta cttaaatatt tacctacaaa agattacttt gcctatttgt
    11161 tagttggttt cgttttggca gtttatttcc aaataccaat tttagggatt tcgttacttg
    11221 gcgctgcaat cgcactaatc gtatataaaa atagatcaaa taatactcaa gtagcaccag
    11281 ctgcaacagg aggttttgac gaagatgaat aagaaactga caaaaatcga caaacaagta
    11341 attttccggt ggattctcaa tggtgcaact tcattaaatt atgaaaaaat ggaagggttg
    11401 ggttatgcgt attctatgct tccttttatc aaggacacat ataaagatga tcctgaagga
    11461 atgaaacaat ccgttttagc tcacctacaa ttttttaaca ctacgccgta tacagcgcct
    11521 tacattattg gtatgaatgt tggaattgaa aaagaagaaa aaaataagtc tttggaagca
    11581 gtaagtgctc tgaaaacagg tctaatgggt cctttagcgg gaattggaga tagtgtattt
    11641 gtagttattc catggactat ttttggagca attgcagcta atatggcatt ggaaggtagt
    11701 tttgttggaa ttattttgtg gatactggca tcaattttat taaggattac tgcttatcct
    11761 ttataccttg ctgggaaaga atcgggtgcg agcctactaa ctcaaattga aacaaaatta
    11821 aagtctatta ctgatgcagt atcaatttta gggttaatgg tggtcggtgc actcattgcc
    11881 acagttgtaa aagtgaatgt tggctttgaa tttaaacaag gcgacttaac aatgaccgga
    11941 aatgaaatct ttgatcaagt tatgcctggt ttattacctg catgtgtagt agcaattgtg
    12001 tattatttgt taggacgaaa ggttaaacca gtttatgtaa ttctattaat cttagtagtt
    12061 tctattgcgt taagagcgtt aaatattttg aagtaggtgt tgaatatgga tgaaaaaatg
    12121 aaaggtatta tacatgttcg tattgatgat cgaatgattc atggacaagt agcaacacaa
    12181 tggacaaatc aattgagtgc taacagaatt atggttatta ataatgaagt agccaatgat
    12241 gacgtaaaaa aagctgtagt tcgcctcgct gcaccaccta atgttagaac ttcaataatt
    12301 acaagggaag tagcagttaa aaatattctt tctggtaaat atgaaggaca aaaagttctt
    12361 attgttgcag tttctccaat cgatgttaaa tttttgattg ataacggttt accaataaca
    12421 agtgtaaacg taggaaattt atcaagaagg aatggaacag aacgcatccg tccgacgata
    12481 aatattactg aagaagagag agaggctttt aaagaactaa ttagtgatgg agttgaagtg
    12541 acagtgatac aaactcctag agattcaaaa gaatttttaa aggattttat ttattaagaa
    12601 cagaggatga gtggatggag tttataatta catctcatgg aaatattgcc attggatttg
    12661 tagatacatt aaaaatgttt tttggcgata atttaaattg tcatgtggta acacttggag
    12721 atgcaggcat tgaagaattc cgtgaaaaca ttgaaaaaat agtttctcaa gtaaaaaatc
    12781 aagaagttat ggtatttgct gacttgattg gtggaacacc tttcaacgaa tttgctaaaa
    12841 gaagtgaagt atttcaaaat gggtttcaac ttcttggtgg catgaattta ggaattttga
    12901 ttgaggctgt caatttgaga ttacaaggaa aaaaaagcga tgatatcatc gaaaatctaa
    12961 gaaatatgaa tacaattgcg tgtcttgcag atatgcaaag acatactaac gaagaggatg
    13021 agtgataaat gcaaaaaatt ttttacaagc ctaaagattt ttggtttggg gattgcatgc
    13081 ctttttataa agatggaact ttttatctat ttcatcaacg agatacgaga aatcctaaac
    13141 ctttaactga tccttttggg tggtcactag ttacaaccac tgattttgtg aaatttgaag
    13201 aatacgggga agtaattcat cgtggtggca acgatgacat tgatcaatac atttacgctg
    13261 gaagtattgt ccaaggaatc aatgagtatt tagctttgta tactggacat aatcgcaaag
    13321 ctaagttgga tggaaaaact tcagaagtgt taatgattgc aagttctaaa gatttaattc
    13381 attggaaaaa ggatggcgaa gcagcaaaac ttgttcctca agaaggatat gataagaatg
    13441 attggcgcga tccttttgca atttttgatc aagatcatgg aaaatacgtt ttgatactag
    13501 gagctagaaa accagcaagt aagaaaaagc cgacggggag attggtctat tttgaatccg
    13561 aagatttaaa agattggaat ttcaaaggag actactgggc taataatgaa tttaacatga
    13621 ttgaaatgcc tgatatattt gaaattgatg gaacgtggta tttacttttt tcagagtatt
    13681 gtgaagataa aaagactaaa tatcgagtcg gagcaggatt attttcagat tggaaatcac
    13741 cagtagacga agctttcgac ggaaaagcct attatgcagc aagaacagtc ggaactaatg
    13801 aaactggccg ttttctaact ggctgggtgg caacaaaaga aggggagaac gatcaaaata
    13861 actgggattg gggcggtgca cttgttccac atcaaattat tcaaaagaca gacaaaactc
    13921 ttggtgttaa actgcctgaa aaaataattg agaattttaa agaaatcact ccattttcaa
    13981 gttttaagtt agagaaagaa tttggaaaaa aagaaagagt aattgttcct aaaatcgaag
    14041 attattttct atttgaaaca actttgacta ttacatcgtt aacaaaggag tttgctttaa
    14101 aattatttgt agatagtgat actcaagaag gatacgaatt tcgtatttca ttgattaata
    14161 atcagcttac tttcggaaaa actccgaact atccttggcc tcaaatgttt gataaggggc
    14221 tgcaacgacc actatttttg gaaataggca aagaatatga actaaagtta attgttgatg
    14281 atacgatagc tgttttatat attgataatg ttgctttaag tactagaatg tatgataaag
    14341 tcggaacagc attaagtatg actgttacgg agggagcaat caaggtagat aaactagact
    14401 attcaataaa ttatgaatgt ggattttaaa acaattgaag ggatgatgta agtgaaatta
    14461 aatggtttac aacatattgg aattccaaca aaagattttg gagcttcaaa aatcttttat
    14521 gaatccttag ggtttagtct gattaatgaa gaaatgaatg tgggaagtaa agtcggcttt
    14581 ttcgagttga atggaacgat aatagaaata tgggaagata aaactactga gtgtagagga
    14641 gccattgatc atctagcatt agatacggat gacatagatg aggtattcaa ttcaattcaa
    14701 gatttaggat atgaattatt agataaaaat attatggaat taccgttctg ggaaaatgga
    14761 atccgttact ttaatttcta cggaccaaat catgagatta tagagataag ccaaatgaag
    14821 aaataaatag ataacgaagt gctctaagtt ggtatgaacg aaaagtactt cattttttgg
    14881 tatcagcatt tcaatagttt aatgagaatt tactatttca aattaattca aagtgacagt
    14941 attttttact tatttggttt caaattttgt aatccttcct atttattgga atacactcca
    15001 cattttgtat acaagtcata tcatgtttta gcaaagctct taccaagcaa agttataatg
    15061 atgttttcca aagtttgatc tttcgatgga ggctaacaca tattttttgc aggacagcca
    15121 aaattagatg tatagaatag agtctgtgag gcgtatagac agttt
//
LOCUS       DACDUU010000071        14625 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.071, whole genome shotgun sequence.
ACCESSION   DACDUU010000071 DACDUU010000000
VERSION     DACDUU010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.071"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            4..816
                     /locus_tag="IWL30_002264"
     CDS             4..816
                     /locus_tag="IWL30_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAP9706995.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="IWL30_002265"
     CDS             813..1613
                     /locus_tag="IWL30_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAP9706996.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="IWL30_002266"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="IWL30_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAP9706997.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="IWL30_002267"
     CDS             3110..4819
                     /locus_tag="IWL30_002267"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAP9706998.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9389
                     /locus_tag="IWL30_002268"
     CDS             5031..9389
                     /locus_tag="IWL30_002268"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAP9706999.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9534..10007
                     /gene="rimP"
                     /locus_tag="IWL30_002269"
     CDS             9534..10007
                     /gene="rimP"
                     /locus_tag="IWL30_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAP9707000.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10030..11205
                     /gene="nusA"
                     /locus_tag="IWL30_002270"
     CDS             10030..11205
                     /gene="nusA"
                     /locus_tag="IWL30_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAP9707001.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11227..11520
                     /locus_tag="IWL30_002271"
     CDS             11227..11520
                     /locus_tag="IWL30_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAP9707002.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11517..11828
                     /locus_tag="IWL30_002272"
     CDS             11517..11828
                     /locus_tag="IWL30_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAP9707003.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11841..14147
                     /gene="infB"
                     /locus_tag="IWL30_002273"
     CDS             11841..14147
                     /gene="infB"
                     /locus_tag="IWL30_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAP9707004.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14174..14521
                     /gene="rbfA"
                     /locus_tag="IWL30_002274"
     CDS             14174..14521
                     /gene="rbfA"
                     /locus_tag="IWL30_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAP9707005.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         4983 a         2598 c         3255 g         3789 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgagtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccacatccag ctaggacgaa
     5701 atattccagc cgatgaacca acgacaccaa tgatcagtat cgttgaagaa gaacgaagag
     5761 tcacacttga ggggtacgta ttcgacaaag aagtcagaga actacgttca aaacgcaaga
     5821 tcttgacttt gaaaatcaca gattatactt catcatttat cgtcaaaaaa ttctcgaacg
     5881 gcgaaaaaga tgaacaagta tttgatgcga tcagtgtcgg tagctggtta aaggtacgag
     5941 gaagtattca agaagatacg tttgtccgtg atttggtcat gaatgcgcaa gatatcatag
     6001 aagtcaaaca tacacctcgc aaagattatg ctcctgaagg ggaaaaacga gtagagcttc
     6061 atgttcatag caatatgagt acgatggatg caacaaacag catctctgat cttgtggcac
     6121 aagcagggaa atggggacac cgagctatcg ccattacaga ccacggtggt gcgcaagcct
     6181 ttccggaagc acatagtgct ggaaaaaaag caggtgtgaa aatcttatac ggcgtggaag
     6241 cgaatgtcgt agatgatggt gtcccaattg cttataatga tgcacatgaa gcattgagtg
     6301 aagccacata tgtcgtattt gacgtggaaa cgacaggatt gtctgcagta tatgacacga
     6361 tcatcgagct agccgcagtc aaaatgtata aaggaaatgt gatcgaaagc tttgatgaat
     6421 ttatcgatcc aggacatcct ttgtcacgaa caacggtaga tctgactggt atcacggatg
     6481 gaatggttcg aggatcaaag tcggaagaag aagtattgcg gatgttcctt gaattttcaa
     6541 aagatacaat tttggtagcc cacaacgcgg cttttgatat ggggttcttg aatactagct
     6601 acgcaaggta tggtattcct gaagcggcca atccagtcat tgatacgttg gaattagcta
     6661 gatatctgta tcctcaattc aaacgtttcg gattaggtgt gttatctaaa aaatttggtg
     6721 tcagcttgga acagcatcac cgagcaatct atgatgctga agcaactggt cacttagcat
     6781 ggatctttgt gaaagaggca atggacaatc ataacatgct ttatcatgat cagctaaatg
     6841 agcatatcgg agaaggagat tcttacaaaa gggctcgacc tttccatgta acgattttag
     6901 caaaaaatca ggcaggactg aaagatttgt tcaaactgat ttcaatgtcg aatgtcgaat
     6961 acttcgaacg cgtcccacga ataccacgct cccaattgaa aaagatgcgt gaaaatctat
     7021 tgatcggctc agcttgtgat aagggagaaa tattcgaagc catgatgcaa aaaggggtag
     7081 aagaggcaag aaaccgagca aaattctacg attatattga agttatgcca aaagcggtct
     7141 atgcgccttt gatcgagcaa gaattggtaa aaaatgaaca tgatttggaa gaaattatcc
     7201 aaaatttggt agaaatcgga aaaagtttgg ataaaatcgt tgttgcaaca ggtaatgtac
     7261 attatctgaa cgaagaagat gccatttacc gaaaaatttt gatcaattct atgggcggag
     7321 cgaatccgtt gaatcgtcat agtttgccgg atgtccattt ccgaacaacc gatgagatgc
     7381 taactgcatt tcacttttta ggggaagaaa cagcgaaaga gatcgtagta gaaaatacga
     7441 ataaaatcgc agatatctgc gaagaagtga ttccagtcaa agatgaactt tatacaccga
     7501 agattccagg ttcagaagat gaaatatcag aattgagtta cacaaaagca aaacaaatgt
     7561 acggagatcc gttacctgag atcattcaaa aacgattaaa aaaagagttg aattctatta
     7621 atggtaatgg cttctcggtt atttatctga tcgcccaaaa actagtgcat aaaagtaatg
     7681 aagatggcta tctagtaggt tcacgtggct ctgttggctc tagctttgtt gcgacgatga
     7741 caggaatcac tgaagttaat ccgttagccc ctcattatta ttgtccagag tgccaatatt
     7801 ccgagttttt tgaagatggt acgtatggtt ctggatttga catgccagaa aaacaatgtc
     7861 caaaatgtgg tgcacgctta aacaaagacg gtcatgatat tccatttgag acatttttag
     7921 gattccatgg agacaaagtg cccgatattg atttgaactt ctcaggtgat tatcaagcag
     7981 aagcccataa ctatacgaaa gtattgtttg gcgaagacta tgtttaccgt gccggaacga
     8041 ttggtacagt cgccgacaaa acagcatatg gctatgtaaa aggatacgaa agggacaata
     8101 atctgcagtt tcgaagtgca gaagtcgaca gattagcaaa aggcgctact ggagtcaaac
     8161 ggacaaccgg ccagcatcca ggggggatca tcgttattcc cgattatatg gatgtctatg
     8221 attttacgcc gatccaatat ccagcagacg atcaaaattc ggaatggaaa acgactcatt
     8281 ttgattttca ctcgattcac gataatgtat tgaaattaga tattctaggg catgatgacc
     8341 caactgttat tcggatgctt caagatttat cagggatcga tccacaaacg atccctacgg
     8401 atgacccgga agtgatgcgg atatttgccg gtcctgaagt acttggagta agtcaggaac
     8461 aaatttattc caaaacaggg acattaggta ttccagaatt cggcactcgt tttgttcgag
     8521 ggatgctaga agaaacgcat cctacaactt ttgcagaact actgcaaatt tccggactat
     8581 ctcatggtac agacgtatgg ttaggaaatg cggaagaatt gattcgacga ggagatgcga
     8641 cactggcgga agtaatcggc tgtcgtgatg atattatggt ttatttgatc catgctggat
     8701 tagatagcgg gatggcattt aaaatcatgg aaactgttcg taaaggacaa tggaacaaga
     8761 tccctgatga attgcgagaa acctatctat cggcgatgaa agaaaataat gtgcccgact
     8821 ggtatatcga ttcttgttca aaaatcaaat atatgtttcc gaaagcccat gctgcagctt
     8881 acgtattgat ggctcttcgt gtcgcttatt tcaaggttta tttccctatt ttgtattact
     8941 gtgcatactt ttctgtacgt gcagacgact ttgatctagt ctccatgtgc aaaggaaaag
     9001 atgctgtgaa acaagcaatg aaagaaatca cggacaaagg attagatgct tccgtaaaag
     9061 aaaagaatca actgacagtg ctggagttag ccaatgaaat gctggaacga ggattcaaat
     9121 ttggaatgat tgatttgtat aaatcggatg ccgttaactt tgttattgaa ggtgatacgt
     9181 tgatcgcacc atttagagct gtcccaagtt tgggaaccaa cgtggcaaaa caaatcgttg
     9241 aagcaagaaa agacggtcca tttttatcca aagaagatct tgccacccgc ggtaaagttt
     9301 caaaaacatt gatcgaatat atgaacgaca atggcgtatt gaaagatcta ccagatgaaa
     9361 atcaattatc gttattcgat atgctataag catctgtttt cctgtctgct tgtaatttta
     9421 agcagagtat ggtatagttc ttataggtaa tgatactaac tgtgagtgag cggatttttc
     9481 gctcactctt tttaatggaa ttacgcactt aattttttaa ggaggcgaaa cttttgagta
     9541 gcgtcgttga aacagtcaca gaaatggtga caccgatttt agatgaacaa aattttgaac
     9601 ttgtcgaagt cgaatttgta aaagaaggaa aaagctggtt cttacgagta tttattgaca
     9661 aagaaggcgg tatcgatatc gaagaatgcg cctttgtcag tgagaaactg agtgaaaaac
     9721 ttgatgcagc agatccagat ccgattcctc aagcttattt cctggaggta tcctccccgg
     9781 gagcagagcg gccattgaaa aaagaaagcg actatgaaaa ggcacttggt gattatgtcc
     9841 atgtctcctt ataccaacca gtagatggcg aaaaacaata tgaaggattt cttcaatcat
     9901 ttgatgctga acagcttaca ttgaaaatac gcataaaaac acgagaaaaa gaattgactt
     9961 ttgaccgaaa aaatattgca aaagctcgct tagctatcca attttaatca cggagaggaa
    10021 tcagaaaaaa tgagtaaaga aatgttaaat gcgttagacg ctttagaagc tgaaaaaggg
    10081 atctcaaaag aaatcgtgat cgatgcttta gaagcagcat tggtttctgc atacaaacgt
    10141 cactatggcc aagcacaaaa cgtcgaagtg gaatttgatc agaaaaaagg aaagatccat
    10201 gtctatgcgg taaaagaagt aacggaagaa gtgatggatt cacagttgga agtctcattg
    10261 aaagatgcac ttttaatcaa tccagcatat gagatcggtg atacgatccg ttttgaagta
    10321 acaccaaaag attttgggcg gatcgctgcg caaacggcta aacaagttat tttacagcgt
    10381 gtacgtgaag cagaaagaac gattatttat aacgaattca gtgcttatga aaaagacatc
    10441 atgcaaggaa tcgtcgaacg ccaggataaa cgttatattt atgtcaatct tggtaaaatt
    10501 gaggcagttc tttctaaaca agatcaaatg ccaaatgaat tttatcaacc acatgatcga
    10561 atcaaagttt atgtttcccg agtagaaaat acgtctaaag gtccgcaagt atttgttagc
    10621 cgcagccatc cagacctatt gcgccgctta tttgagcaag aagtaccaga agtctatgat
    10681 ggaatcgttg aaattgtcag catcgcccgt gaagctggcg atcgttcaaa agtggcagtt
    10741 cgctcaaatg atccaaatat cgatgcagtc ggtacatgtg tcgggccaaa gggacaacga
    10801 gttcaagcaa tcgtcaatga attaaaaggc gaaaacatgg atatcgtcga atggaatgaa
    10861 gatccagctg tattcattgc aaatgcattg aatcctgcac aagtcatgga tgtgatcttc
    10921 gatgagacaa atccaaaggc ctgcacagtt gtcgttccag actaccaatt atcgctagca
    10981 atcggtaaac gcggacaaaa cgcacgttta gctgctaaat tgactaacca taaaatcgat
    11041 atcaaatcag aatctgacat gacggaattt tatgaaaatc aagaacaaca aaaagaaaca
    11101 gaagaactgc atgatgaagc aatcgttcag tcagatttaa cagacgatga atatgaaaca
    11161 attgcattca acaatgaaac ggtcgaagaa aaaccagaag cttaacttta gtaaggaggc
    11221 aaagagatga aacaaaggaa aattccttta cgcaaatccg tggtttcagg tgaaatgaaa
    11281 cctaaaaaag agatggtccg aatcacccgt tcaaaagaag gggaagtatc gatcgatcca
    11341 actggaaaaa tgcctggacg aggagcgtat gtctcacttg agccggaaga agtacaacaa
    11401 gcttgggata aaaaaattct cgatcgagta ctcgaaacaa cattaacaga tgagttttat
    11461 caagaacttc ttgactatgt cactcaccaa aaagcacgta aagagttatt tggaaaatga
    11521 atacagttaa tcggcaaaaa gccatgaatc tcatcggtct agcgatgcgc gcgggaaaac
    11581 taatcacggg tgaagaactg accattgctg aaatccgcaa acaaaaagca aaattggtgt
    11641 ttgtggcaac cgatgcaagc gagaatacaa gaaaaaagat aaaagataag agttcatatt
    11701 atgaagttcc ttgctttgag ctattttctg aagcagaaat cacgcagatg atcggaaaac
    11761 ctcgcaaggt attcggaata atggatgccg gatttgcgaa gaagaccaag gagctaattg
    11821 aaggttagga aggtgattgc atgggcaaca aaagaatcta tgaattagca aaagaactca
    11881 aaaaatctag taaagacgtc gtggaaaaag cacaacaact tggcatcgat gtgaaaaacc
    11941 atatgggtgc gattactagc aatgatgaga agaaattgca acaagcgttc aaaaacccaa
    12001 gcacgaaaca acaagctgca caacaagcaa gcagaaaacc agccaatcaa cagccgaacg
    12061 atcaacgtaa aaaagaaaca acgaacaatc aacaaaaaaa taaaaataac cgcagttatc
    12121 aagatcgcgg tcaagggagc ggtcaagtga atcaaggaaa aaaaccaaca acaaaccaaa
    12181 ataatacggg aaataccagc agccaaaatc gtcaaggaac gagccaaggc ggcaatcaaa
    12241 accgtactaa caataacaac aaaccaaaca ataacaatcg cggcaaattt aataataata
    12301 accgtaatcg ttataataaa aaaggtaaaa aaggcaaaca gcaacaatca actaaaccag
    12361 ctgtaccacc acgcaaattc cgtgaattgc cagatgtatt agagtatact gaaggtatga
    12421 acgtagcaga tattgcgaaa aaaattcatc gtgagccagc agaaatcatt aaaaaattat
    12481 ttatgcttgg tgtcatggtc aatcaaaacc aagcactgga taaagatacg attgaactgt
    12541 tagcgacgga ctatggcatg gaaccacaag aaaaagtcca agttgacatt gctgatatcg
    12601 ataaattctt cgaggcagaa gaagtcgatc cagataaatt agtctcacgt ccgccagtcg
    12661 taacgattat gggacacgta gaccatggga aaacaacttt attggataca ttacgccatt
    12721 cacgtgtaac aagcggcgaa gccggcggaa tcacacagca tatcggtgcc taccagatcg
    12781 atattgatgg taaaccaatc actttcttgg atacaccagg acatgcggcc tttacaagta
    12841 tgcgtgcacg tggagcaagt atcacagata tcacgatctt agttgttgct gcagatgatg
    12901 gcgtcatgcc acaaacagtt gaagcaatca accatgccaa agctgcaggc gttccaatca
    12961 tcgtcgctgt aaataaaatt gataaaccag gtgcaaaccc tcaacatgtt atgcaagaat
    13021 taagtgagta tgagttaatt cctgaagcat ggggtggaga aacgatcttt gttgagatct
    13081 cagcgaaatt cggccaaaac atcgaagaat tgctggaaat gatcctgtta gtcgcagaag
    13141 tagaagattt aaaagcagat ccaacacaac gcgcaatcgg aacagtgatc gaagcacgtc
    13201 ttgataaagg taaaggtcct gtgtcaacct tacttgtcca agaaggaaca cttcatgttg
    13261 gtgacccaat cgttgtcggt aatacttacg gacgtgtccg tgtaatgaca aacgatcttg
    13321 gccgtcgcga taaagaagca ggacctgcaa cgccagtaga aatcactggc ttgaatgatg
    13381 tacctcaagc aggtgaccgt ttcgtagtct tcgaagacga aaagacagca cgtgcagcgg
    13441 gtgaagaacg cgcgaaacgt gcattattag aaaaccgtgc tgccacaagc cgtgtgactt
    13501 tggataactt atttgaaagc ctgaaagaag gcgaattgaa agaagttaac gtaatcatca
    13561 aagcagatgt acaaggttca gcagaagcac tggctgcatc attgaagaag attgatgtgg
    13621 aaggtgtccg tgtaaaaatc gtccattcag ctgtcggagc aatcaatgaa agtgatgtaa
    13681 ccttagcagc agccagcaac gctattatca tcggtttcaa cgttcgtcct acaccgcagg
    13741 caaaagtgca agctgatact gaagaagtcg atattcgtct tcaccgtatc atctataaag
    13801 ccatcgaaga aatcgaaact gcgatgaaag ggatgctaga ccctgaattt gaagaaaaaa
    13861 tcaccggtca aatgatcgtt cgtgaaacct tcaaagtttc taaagttggt acgattgcag
    13921 gtgcctatgt aacagaagga tatattcgta gagatagcgg tgtccgtgta atccgtgacg
    13981 gaatcgtaat ctatgaaggc caacttgcaa gtctgaaacg attcaaagat gacgtgaaag
    14041 aagtcaaaat gggttatgaa tgtggtgcaa tgattgaaaa attcaatgac atcaaagtag
    14101 atgacgtaat tgaaggattt atcatggaag aagtcaaaaa tgactaaaaa ctgataaaag
    14161 gaggccaatc attatggcta actaccgtga ccgccgagtc ggtcaagaaa tcttaaaaga
    14221 agtcaatgat attttgcgca aaaaggtccg tgaccctcgt gtggagaacg taacgatcac
    14281 agatgtgaaa gttacggggg acttacagca agcaacgatc tattacagca ttttatctga
    14341 cttagcatca gataagaaaa cagcacaaga tggattgaat aaagcatcag gattgattcg
    14401 tcgagagtta gggcacaatc taagtattta taagacacct gaattgttct ttgaacttga
    14461 tgaatcagtt gtctatggca accatatcga tgaattgctt cgcgacttga acaaagacta
    14521 atcaaaaact cccgcaaatc ctgtgaaatc aggctctttg tcaataagga ctgatgagtt
    14581 gtgcaaaatc aaatctgagt cagaatgaac cacattctgg ctcag
//
LOCUS       DACDUU010000072        14472 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.072, whole genome shotgun sequence.
ACCESSION   DACDUU010000072 DACDUU010000000
VERSION     DACDUU010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.072"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(277..639)
                     /locus_tag="IWL30_002275"
     CDS             complement(277..639)
                     /locus_tag="IWL30_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707006.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(713..1093)
                     /locus_tag="IWL30_002276"
     CDS             complement(713..1093)
                     /locus_tag="IWL30_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAP9707007.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1175..2350
                     /locus_tag="IWL30_002277"
     CDS             1175..2350
                     /locus_tag="IWL30_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9707008.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2426..3010)
                     /locus_tag="IWL30_002278"
     CDS             complement(2426..3010)
                     /locus_tag="IWL30_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9707009.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3211..4209
                     /locus_tag="IWL30_002279"
     CDS             3211..4209
                     /locus_tag="IWL30_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9707010.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4321..4830
                     /locus_tag="IWL30_002280"
     CDS             4321..4830
                     /locus_tag="IWL30_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAP9707011.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4860..5519)
                     /locus_tag="IWL30_002281"
     CDS             complement(4860..5519)
                     /locus_tag="IWL30_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAP9707012.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5521..6285)
                     /locus_tag="IWL30_002282"
     CDS             complement(5521..6285)
                     /locus_tag="IWL30_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAP9707013.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6293..6970)
                     /locus_tag="IWL30_002283"
     CDS             complement(6293..6970)
                     /locus_tag="IWL30_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAP9707014.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7007..8392)
                     /locus_tag="IWL30_002284"
     CDS             complement(7007..8392)
                     /locus_tag="IWL30_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP9707015.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTIQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8602..8686)
                     /gene="ffs"
                     /locus_tag="IWL30_002285"
     ncRNA           complement(8602..8686)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IWL30_002285"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8709..9224)
                     /gene="tadA"
                     /locus_tag="IWL30_002286"
     CDS             complement(8709..9224)
                     /gene="tadA"
                     /locus_tag="IWL30_002286"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAP9707016.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9331..10188
                     /locus_tag="IWL30_002287"
     CDS             9331..10188
                     /locus_tag="IWL30_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAP9707017.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDGTEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10269..10604)
                     /locus_tag="IWL30_002288"
     CDS             complement(10269..10604)
                     /locus_tag="IWL30_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707018.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10671..12041)
                     /locus_tag="IWL30_002289"
     CDS             complement(10671..12041)
                     /locus_tag="IWL30_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAP9707019.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12119..12655)
                     /locus_tag="IWL30_002290"
     CDS             complement(12119..12655)
                     /locus_tag="IWL30_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9707020.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12964..14445
                     /locus_tag="IWL30_002291"
     CDS             12964..14445
                     /locus_tag="IWL30_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9707021.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4458 a         2882 c         2353 g         4779 t
ORIGIN      
        1 tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt tatcatgtat
       61 ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt cgatcaatta
      121 ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca ctaaaaaact
      181 atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa gaagtttgaa
      241 aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca ctccctctac
      301 ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat atccttcgcc
      361 taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa cattttcctc
      421 tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga tatgaactat
      481 ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa gttccgctaa
      541 cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac catcctccaa
      601 atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct cgatcaactt
      661 gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact ttttatttct
      721 tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt aactgctccg
      781 caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt ctgcgatatg
      841 gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt ttggaatatt
      901 catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat gaatggatat
      961 ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc tcgttatcaa
     1021 gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt atttgtccaa
     1081 ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa atagctcaaa
     1141 aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata aacagatttc
     1201 aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc aaatggatga
     1261 cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg tagctaaagc
     1321 gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg ataaactata
     1381 tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga aagaaaatat
     1441 cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt ttacaaaacc
     1501 aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta ttatagaaac
     1561 aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt tcatcatgga
     1621 cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga ttttatgcaa
     1681 tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt taagcgaatt
     1741 gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt taactttatt
     1801 cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc tactagttgc
     1861 ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa tggtatttat
     1921 caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc aacagcacga
     1981 aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg gagaatggtt
     2041 aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt tttttgaaca
     2101 gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt ggttagattt
     2161 taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga aaggaaaagt
     2221 cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc gcttgaatat
     2281 tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg ctttagagac
     2341 aatcgattaa atatttattc aaaataaaag gacgagacct tactaggttt cgtccttttt
     2401 cataattact ccgtaccata ccaaactatt ctgaaatttc aattgtttca atcactacat
     2461 catgtacagg tcggtcttgt ggaccacgtt gtacatttgc gatttcatct actgtgtcca
     2521 tcccttctag tacatgtcca aagactgtat gtcggaaatc tagccaagga gttccgccac
     2581 ctttatacgc ttcaatgatt tctgatggga atccagcacc ttctagttgt ccaagcatat
     2641 tagctggcac atttgtgtta tttacaataa agaattggct tccgtttgtg tttggacctg
     2701 aatttgccat agataaagct ccgcgaaggt tgaacagttc acgactgaat tcatcttcaa
     2761 aactttcgcc ataaatactt tcgccaccca ttcctgttcc agttggatcg ccgccttgga
     2821 tcatgaaatc aggaataacg cggtgaaaaa tcacgccatc ataataacct tttttggaaa
     2881 gttcaatgaa attttgaacc gtcttcggtg ctagttcagg gaataattga acagtaatgt
     2941 caccacggtt tgttttgata acagcttttg gtcctttttc actttttaaa tctagttgtg
     3001 ggaatgccat attgtttcct cctaatattt gcatacttca taataataat aacataatac
     3061 ctaacctctc gtaaaaatac tagatttgtt ttcttttatt tttcatcact agatacattc
     3121 tataacttac cgtttcgatt tcgaattcaa acatttttga gaagcattct caattaaatt
     3181 ctgctatagt gtgaaaaggg aggaattttt atggaaatct atgacattac aatcatcggt
     3241 gcaggtcctg tagggatgtt tgccgctttt tacgcaggaa tgcgccaagc taaaacaaaa
     3301 atcattgaca gtctacctca acttggtggt caattagcaa ctttgtatcc cgaaaaatat
     3361 atttacgata ttcctgggta tccagcaata aaagcaagtg aattaatcga tcaattagaa
     3421 aaacaattaa ctacttttaa ccatacattc catttaaaag aagaagttct ctcgcttact
     3481 cgtgaagacg aagttatcga gattactaca aataaaggca tccattattc caaagcagtc
     3541 attttagctt taggaaatgg ttcattccag ccaagaaaat tgaatttaga taatgctgaa
     3601 tcatttgaaa accacggatt ggattatttc gtgaatgatt tgatgaagta tgctggaaaa
     3661 aaagtcgcta ttgctggagg tggtgattct gctatcgatt ggacgttgat gttagagcct
     3721 attgcaagcg aagtttactt gattcatcgt cgcccggaat ttcgtgcgca cgaacatagt
     3781 gtatcacgct tgaaatcttc atctgtcaac ttgcttactc cctatctcat cgatggatta
     3841 tcaggcaata atggggaact gacagatatc cggttaaaaa aagtgaaaag tgacgagacg
     3901 attgatttga tgattgattc tctgatcgtc aattatggat tctcttctaa tttagaacat
     3961 ctttcttctt ggggacttga tagtaccaga aatgccatta ctgtaaaatc agatatgtct
     4021 acttctattc caggcgttta tgcagctggt gacatctgta cctatgaagg aaaagtgaaa
     4081 ctgattgcta ctgggctcgg tgaagcacct actgcagtga acaacgcttt acactatatt
     4141 aatccaaagg aacgaactca gcctggtcac agcacaagct tatacgataa aaatcttcgt
     4201 cctagttgat tttttatatc tctttgcatt tcaagttaaa cccgaaagca gtttcttgca
     4261 tttttttcat gttccgttaa catgaaatag agaaagaaaa aataaagggg gagcaataat
     4321 atggtcgtca aaactgagac tttagaagaa aaggcaagag aactattgaa ggaacgtggc
     4381 gtaactgtag aagatatcgc acaacttgtc atgtttttac aaaaagacta tatttctgat
     4441 ttgactttgg cggattgtac tgaaagtgtc aacagcgtgc taacaaagcg tgaagtacat
     4501 aacgccatta tcactggaat ccaattagat atattagcag aaaagaaaaa attgatgagt
     4561 ccgttgcaac atattatcga agatgacgaa ggcctttacg gtatcgatga gatcctagcc
     4621 ttgtcaatcg tgaatgttta cggtacgatc ggctttacga attatggtta tatcgacaag
     4681 gtgaaacctg gtattttaaa agaactaaac agccacgatg gcgttcatgt ccataccttc
     4741 ttagatgata tcgtcggagc gatcgccgct gctgctgcca gtcgattagc acacaaagaa
     4801 ccagaaaaaa gaagtcgcct tactcaataa ttcaacaaaa aaagaaagaa aacgtctatt
     4861 tagacgagtt ctttcttttt tgtttgtttc aagctatttt ttcctaagaa aagaaaaaca
     4921 gcaaaaaata attctaataa tacaaaaaat aagataaagc tatgcatgaa aaattcttct
     4981 ggaagtacat tgatgattgg atcagtaacg ggatcgaata accagtcatc gttgttgaaa
     5041 aacgtttcat gaaaaaggat aaaaaagcga tcaaatccta tcatcatgaa aaaaccgaaa
     5101 acaactggca gtaacatgcc gatttgaaac ggacgaatca accgccaaag ccgatcattc
     5161 ttttgcaaat atttgataaa caaaatactc gggatcaaag tgatgaaaaa cacagcataa
     5221 tccactaaaa acaaaatttt cacttcataa aagtgatggg ctccgctagc agaaactggg
     5281 aaatctggca agctgaggat ggttttgaag ggattattga gataattcat taggtgatca
     5341 aaatttttca gtaacgtctc ttgatctaaa gaagtataat ccaaaatttg caaatgtccg
     5401 atatcaaata cgtatagtgg tcgaaagttg atcgtcacac caactgccag actgatcagt
     5461 gtcagaaata ggctgaacaa tccagcatat tccatccact gccatctcga atttttcata
     5521 ttagaagctc cattcatcca gtgattgttt cacataggta gctgggatag gcaacccagg
     5581 tacatcttct tcttttgtaa aacctgataa aacgagcaaa gtatctatat tattgcgtat
     5641 cccagcttgg atatctgttt catagttatc tccaaccatg attacttctt ctttttgcag
     5701 ccctaatact tcaacggctt tatccataat gatggcttct ggttttccga tgtagatcgg
     5761 cggcgtttgg gttgctgtct ctacaaaaga gatgactgac cctgctccag gaagaaggcc
     5821 gcgttcagta ggtatattct tatctgggtt cgttccaatg aaagtagcac ctttttgaat
     5881 agcaagtgtt gctttaacta ctttttcata ggtcagataa ttatctaagc ctaccactac
     5941 atagtcagga gcctcttctt cccatgtaaa tccggcagct aaaatcaagt caatcaatcc
     6001 tgcttctccg ataacataga cttttttccc ttttccatct gctttcataa aatcgattgt
     6061 tgccaaagta gctgtgtaga ctgtctctgg agctacatga atgtcaaact catctgctag
     6121 acgattagct actgtttctg gtgacttagt cgtgttattg gtaacaaaca agaaaggtaa
     6181 ttttcttttt tgcaattctt ccacaaatcg tttaccggcc ggtatgggtt gtgtcccacg
     6241 ataaatcgtt ccgtctaaat caattaaata tcctttatag tccattcatc cactactctt
     6301 ccctttttct gatcgtaaat gctgtttttg ttttttctac ttgtttaggt tgttcttgtt
     6361 tttttacttt ttcagaggtt tttgctgact ctgtttttcg atttttaata accggttttt
     6421 tgcgattaga tgcgactggt ccttttttct catcgatata cgcacgatta tggttttttt
     6481 tcttttttcg gcgctgagtt gtcttttctt ttttaccgcc gattctctcg atgacaaaat
     6541 acgcacaacc aaaattgcaa tattcgtaaa gatagtcttc caaggaatcg attcgctgat
     6601 ctggatgcgc ccgtcgatta tccgcttgga aaaatccttt taggcgtagt tgttcataac
     6661 cccaatctcc tacgatataa tcatatcggg ctagtacatc actgtaacgt tctccaagct
     6721 tttctgcatt aaatccttca cgataatcgg acacaagccg ataatgtctt tcacctatca
     6781 aaaaattact gtcatctaac agtgtcacta gctcgccttt gactttttct gctttaggct
     6841 cctcaacgat ttcttccaaa gcagctgtca gttcttcggt gactgttgtt tcctgttcag
     6901 ctgatgattt tttcttagca atttctgttt tatgctcaac gttctgctgt tcattttgtt
     6961 ttgcagacat gcgttcacca cctatctttt ttattatacc ttgtttttat ttgattgggt
     7021 agtgagcttt caagtaatct cctaccacac ttcggataaa ttcagggaac aaaaattcat
     7081 tttttccagc aatctcaatc gttggaaaaa atggaacaaa catcaaatga tctacggtag
     7141 taaaagtata atttttattc agatcgattg gcttgttctt ccaaaagact tgatggttaa
     7201 tagaatcata tgtgattcca ttatagacca tctgcccaaa aatcttaccg cgaaatccca
     7261 ttcccttcat aggatagtta cgtaaaaagt tacgattttt ttctatttct aaaactaagc
     7321 ggatcagatc atttccaccc aatgtgacat ttaacaagtg catcggatgc ggcaacgcag
     7381 tgtgaagctg atcctgattg acccgccctt ttggaatttc agttaaaaac agaccactgt
     7441 ttaatattgc tgcttccgtc tttcctcgtt ctttcaccgc ctcaaaggct acttggataa
     7501 acgaatgctc attgaagata tctagactca gagaaaatgg caaatctgct acttcttttt
     7561 cttttaataa ttcatgccca cgttctagat aactatttat ttcttgttca tcttctgtaa
     7621 aagctgtcat tgtttcggta gggatggctt tggctgaatg cttaaggatt tttttctctt
     7681 catcgatggt taaatggact tcaccgacat actgcccgta ctttccagcg gctgctattt
     7741 ggacatcatt gattacgtga ccttctctga ataaatgatg ggtgtgcgat cctaaaataa
     7801 catcaattgc tggtatttct tttgcaattt ggaaatcatc ctgaatcccg agatgactca
     7861 tcaaaactaa aacatccact tgtgaacgta aatcttcgac taattctggc aaaatatcat
     7921 aaggattccg tatgtcccag ccattcggat tataagtaag cggaaatgga gcagtaaatg
     7981 caattagtcc gattttcgtc tggtgtttcg ttgtaatgat tttatatggt ttcgtccatg
     8041 ccggtggctg gatcgtattt ttatcgaata agttatccag gatgatatca aactcagcat
     8101 gatcgtataa atgattcaac tcttctttgg aatttcctac gccttcattg tttcctatcg
     8161 tcgctgcatc ataaccaact tcattcatca gcaagatatt ttcctgtcca ttcgttgctt
     8221 ctgacaatgg atgccaacga tccacaaaat cacctagatc tactgcaatc gttgtcgttt
     8281 tttctttctt ttcgttttct cgtttacgtt gatccaaaaa ccttctgatc cttggccaat
     8341 tttctaaatg agaatgcaga tcattcgtat gtaaaatcac tattttttcc atgtaccctc
     8401 tcgcctctct ttatctttat cataccatag gcagcttttt cttccattga aacaagctct
     8461 atttcagagt ttttcctgcc ttgaagcaaa ttgtataagt acaatattcg ataaatcagt
     8521 ctggctaaaa aatcaggaac attcattttt acagtactat aaaaataaaa aaaacagacc
     8581 cgaaagtctg ttgtcgtttc attataatca gacacatggc ggaacctgct tagtgctgct
     8641 tccttccaga cctgacacgc ttcacaagcc caccattgtc ctagcctttc ggcaaagaat
     8701 agtatacctt atttttcatt cgttgaccag tcttttttta atttcttttt tcgctggcgg
     8761 agctttcgga aaaagtcagt caaaatcatc ccacattcgt cttctaaaat gccaccttct
     8821 acatatgctt ggtggttaaa ccgctcgtcc tccaaaagat tcatcaaggt accggctgtt
     8881 ccgccttttg gatcatacgc gccgaaataa acttctgcta cacgagacaa aatcatcgcg
     8941 ccgctgcaca taggacaagg ctctaaggta acaaaaagtg cagcatcttc caagcgccag
     9001 cttcccactt tttcacaggc ttccttgatt gcaagcattt ctgcatgtgt agtagcatct
     9061 tgcgtggttt ctctaaggtt atatcctctt ccaatcactt ttccatccaa tacaaccact
     9121 gctccaatcg gaacttcatg aagcatttct gctttctttg cttctgccaa tgcaaggcgc
     9181 atccattttt ctttttctac tgcatcaaga tttgtattta ccaattaccc ttccctgcct
     9241 ttgttttttc ttgttttact ttagcatatg ataaaaaggc tgtaactaac ttttttttct
     9301 atggtaaaat aaatacaaga ggtgaacaag atgaaaatgg ataaattact tgctctttac
     9361 ccacaagcac agaaaaaaaa ctctccagca tcagaagcgg atatcctttc ttttgcaatt
     9421 gatgatgctt ttatctggat caaacaagat tcattatctc aacaagaaac cagccttttg
     9481 aaagcgttat tccctgtaat caacgatcaa aaaaaacacc catggtatcg ctatcttttt
     9541 aaagatgctt cttgtgttca agaaaaaagc tttcgtttga tccagcttca cgtcgaatca
     9601 aaaggagaat tcttaaaaaa agaatggcaa gatactattt tggaattatt tcgctattct
     9661 gctgatttct ttttttatac agacacagat gcgctcttag tcgaacaaat aaaccgtgat
     9721 tatcttgatg gtactgaaat caacggtatt tttctatctt tagatgctga ttttgatacg
     9781 acaacgtctg tattcgttgg ttctttccat tcacctggca ccgacctcgt ccctttgttt
     9841 gctgaagaaa gaaaaatttt tttaacagag caaaacaatc tttatactca cagccgtact
     9901 ttttctatgc aagatgtggc tttacattac tatacaaaag atactgtaaa agatagtaag
     9961 ttgatcaaaa gttacataga actgatcaaa caaaatgaaa tggaagtgat cattcaagaa
    10021 ttatggaatg aattaggcaa tgtcagctct gctgcaaaaa cactgtttct tcatcgtaat
    10081 actctaaaat atcgaatcga aaaatttcaa gaacagtctg gtttcaatct aaaaaaagcc
    10141 aacgatttac tgttttgcta tcttttattg cttcatgaac ataattaaag atagattaaa
    10201 ggggttgtga caataatttt gtcacaaccc cttatttcga atgaatagtg ttcaaaagct
    10261 agtttctctt acatcatttt ttcaacaaaa tgcattagcc ggtcttttac atcttcggtt
    10321 gaatctttga ttgaatcacc ataaacagag attttctttt ttcctgattt gatttttgct
    10381 aacattccca tcaaagaatc caagtcacta tctgaaagac catctacgat ttctagtaat
    10441 ttttcatttc catcaaattt gtcatagacg aattttttta ctttcgtccg agtagtcata
    10501 tgatggatct tttccattac ttttcccgat acaacgactg ctgtcgctac actggcagcg
    10561 gctgctacgc ttaatccgat tgttactttt gttgatgttt tcataagtat cagctccttt
    10621 ttatctatct ccatcatagc atagacaaaa aaataaacga actaatccac tcagtgaaat
    10681 ctcaatcctt tagggaaaaa gttctttact gttcctttta cttgtttccc aaatccgaca
    10741 ggtatcttat ctaatactag caaaacccat ccttgattcc catcacgttg aaatgtttct
    10801 cctgctgtgt aagattgcca ttccttttga gtgatcggca agcaaggtat attctctatc
    10861 ttttttgtag caagcgctaa tgcatagcta ggttcaaatc gattcttttt gaaatctcct
    10921 aaatgcaaac ctgtacgtac gacttttaaa ccatcgagag aaggggctaa ctcaggaact
    10981 tcccaaagat ggtcgttgaa cactaaaagt cttccagtag cttcataatg aaaatcattt
    11041 gaaaactccg tccagagttt ttcctgctct tttgtcattt gcactttact tttttttcgt
    11101 gtcttctttt ctttatgcat ctgattttgt ccatgaaaag tcagctttgc aacaaaatgt
    11161 ccttcaccct gatctttatg aggccatata cgtattgttt tttccaaacc agctacgctt
    11221 ccccattcag aacgtcctga actaacggat tgagtcaaag gaatctcctc aatcgttact
    11281 ggataatttt ctacaagcca gctaatgatc tcttcatttt cttctggtgc aaacgtacaa
    11341 gtcgaataaa tcagttgtcc tttatttttt agcattttta ttgctgaaga caaaatctct
    11401 tgctgacgtt tttgacagta tagcggagat tcttctgtcc actcttttat tgcatttgga
    11461 tcttttcgga acattccttc tcctgaacaa ggagcatcaa ccacgatccg atcaaaaaag
    11521 cccgaaaaat gagggacaag ttctgcgggt gcatgatttg tcactattgc gttcgaaact
    11581 ccccatcgtt ctatattttc tgataaaatc ttcgcacgtt tcggaaagat ctcattcgtc
    11641 actaataacc ctttcccctt catttgagca gctaattggg ttgatttacc accgggagcc
    11701 gcacacaaat ccaaaacttt ttcaccgggt tttgctgcag cagctgttcc aacgatcatt
    11761 gcacttggtt cttggctata ttcataccca gcttgatgga ggaaagactt tccattgaca
    11821 gtgccaagaa atccctcatt gctgtaggga gcgggttgta attcttcgct atggtaagtt
    11881 tggaccatgt ccaatcctgc cggttttagt ggattccacc gaaatccttt tttcacgctt
    11941 ccttgttcaa gcgcagaaaa aaaatcggat gcttcttcgc ctaaaagaag acgatatttt
    12001 ttgataaatt gctgtggtaa cgttgtcgct tcctccttca ttccaatgtt tcgttagaca
    12061 ttttacaatc ttttttacga aaaataaagg tggctgatct actttttcat gttgctgcct
    12121 aaggtgactg ttcctttaag atattttcca agtattcatc cgtcacctcg tttttatccc
    12181 aaacagcgtt catcatttca agaaaatctt gagaagaatc atctgagcgc aaacttccca
    12241 atgcattaag ccataccaat tcaagcgact tttcttcttt gatcttcgtg tgacctttga
    12301 catatgagaa aaatggccgc ttaaatagcg aatccatgtc taaatcagat tttgcaatga
    12361 tttcacgaat actttcatgg atagaaaaaa aatctttcaa taagtagaca atgcttaaaa
    12421 catcatcgga actgcattca gctaagtagt tttcaaaata aacagtgatt tcttctaata
    12481 tcttctcttt taacccgctg attgatttga aatgtgtata aatcgggtat gtagaacaac
    12541 cgcagaactc tgctaactct cttgcagtaa ttgacttcat tccatattct tccacgtata
    12601 ccatactttt gaacagcaaa tattctttac tataatttgt tactctcccc atcattctta
    12661 cctccgtctc ttttcatcta cccataaata aataacataa ataattctaa aaatattatt
    12721 gataattatc aaaacatatt tagttatatc cattaaaaat caatttttcc gccttaaaaa
    12781 caagaatatc ttgacaaaat accctaggaa ataaacaata gaataaacaa ggtaacaaaa
    12841 aaattaaata acgttatata aaaaagaagg cgacactctg tgagaataac acagactgaa
    12901 atgactttcc actcacagtt tttgtctaac tatatacaaa aacagaataa aaaggaggta
    12961 tttatgctaa ttgaagcaag aaaaaaagag ttacttgttc tacaaatggt cattcaaaaa
    13021 aataattgtt ctttacaaga attatccaat gacttacata ttccaaaacg gacaatcaaa
    13081 gaactgatcc gaaaaataaa tgtatcactc aagcaatttt tattgattga tgaatttatt
    13141 tattctaatc ataaaggaga gatccaaacc aaagaaaccg atcaaactaa aaagttagct
    13201 gttttttcta agttaaaatt atgctaccta aaagaatcca atcgttttaa ctatttgatg
    13261 ttactaatca attatccaca aactgctgta ccaaaaaaat atttacttga acaactttat
    13321 atatcgccct cctatctcga aaaactgaca aaacagctca atcattcctt aaaaaaattc
    13381 catttacgga tcctatctgt aaaaaattgc tgtactcttg aaggaaatga gttatctatc
    13441 cgtctctatc tttacttttt tctttcagat acatttggag gtaacgattg gccattttcc
    13501 tcacttgaat taaatgcact aaaaatgaac agtacagatg agcaaataga aaaaatttca
    13561 aaacaagaaa aaaatatcta cttgctgtta actttattca tattacgaaa caaacaacag
    13621 gcatattttc caaaaacttc ttctgaaatc actgaattga tggacttatt gcagcataca
    13681 catgattatt cagaatactt tacttctttt tcagtgagtc gcccttgtga agagacagaa
    13741 acagagagag cctactttaa ttattttttc tattgttttt ttccaaatca aatctctttt
    13801 cgacaaaaac aggagattgg ttttttattt tccacaagca aaaaccttat ttgtcgacaa
    13861 gtcggtcaat tgatgactta cttaactcaa cagtttcctc tcaaccattt agaaaataaa
    13921 ataccaattt atttttattt tcttgtgttg gcagttgttt ttttcgaact gttgggtgaa
    13981 tccagcagta gtttttctac actttgcttt ccagaagcca cgtccaatca ttctagttct
    14041 tttatacagg aaaagaaatt aagagaaaaa atcgatacaa ttttttcaaa aaatggggaa
    14101 agaaaccaaa tcagtcaata ccttagtcaa ttgatccaac acttactaca aacagaaacg
    14161 aaagagcagt tactcatcca tttgcagata accaaacagt ttacaggaaa ttatcatctt
    14221 caaagccatt tgcagcagtt atttcgcaaa gatgctgtta aaattaccgc atattcagaa
    14281 catgctgatt tgatcattac agatcagcat gagaaaattt cagatgaaac ggagactttc
    14341 tatctggata caacgcacaa tacagtgtta tttggcaagt taataaaagc ggttcatgaa
    14401 ctgtatatga aaaaaggtta tgagaacaaa acaaagagga tgtgacaaaa ttcttgtcac
    14461 atcctcttat tc
//
LOCUS       DACDUU010000073        14083 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.073, whole genome shotgun sequence.
ACCESSION   DACDUU010000073 DACDUU010000000
VERSION     DACDUU010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.073"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..3648)
                     /gene="rpoC"
                     /locus_tag="IWL30_002292"
     CDS             complement(<1..3648)
                     /gene="rpoC"
                     /locus_tag="IWL30_002292"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAP9707022.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQT"
     gene            complement(3727..7344)
                     /gene="rpoB"
                     /locus_tag="IWL30_002293"
     CDS             complement(3727..7344)
                     /gene="rpoB"
                     /locus_tag="IWL30_002293"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP9707023.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8072..9040
                     /locus_tag="IWL30_002294"
     CDS             8072..9040
                     /locus_tag="IWL30_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9707024.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9058..9963
                     /locus_tag="IWL30_002295"
     CDS             9058..9963
                     /locus_tag="IWL30_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9707025.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            9960..10766
                     /locus_tag="IWL30_002296"
     CDS             9960..10766
                     /locus_tag="IWL30_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9707026.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10923..11891
                     /locus_tag="IWL30_002297"
     CDS             10923..11891
                     /locus_tag="IWL30_002297"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAP9707027.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12208..12732)
                     /locus_tag="IWL30_002298"
     CDS             complement(12208..12732)
                     /locus_tag="IWL30_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAP9707028.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13019..>14083
                     /gene="scm"
                     /locus_tag="IWL30_002299"
     CDS             13019..>14083
                     /gene="scm"
                     /locus_tag="IWL30_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP9707029.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         3982 a         3040 c         2787 g         4274 t
ORIGIN      
        1 tgtttgattg ttcattgcgt cttctgcagc catttgcgct tcaatatctg aaatactgta
       61 gacattttcg cttgctacac cgacttcttt tggttccatg tcacgataac gttgcatacc
      121 agtaccagct gggatgatct tacctataat aacattttct ttcaaaccaa gaagaggatc
      181 tttcttaccg cggatcgcag catccgtcaa gacacgagtt gtttcttgga atgatgcagc
      241 agataagaaa ctatttgttt ctaatgatgc ttttgtgata cctagtaaga caggtcgaga
      301 agttgcagga acgcctcctg agactagcgt attgtagttt tgatctttaa agtctgcgat
      361 gtctaataat gtacctggta agatatctgt atcacctgga tccattacac gaactttacg
      421 cagcatttga cgaaccatta cttcgatatg cttgtcgccg atttctaccc cttgcatacg
      481 gtaaacacgt tgtacttcac gaagcaagta gttttcaaca gataatacat cgcgaacttg
      541 caacaattgt tttggatcga ttgatccttc tgtcaatggt gcaccacggt ggatcatatc
      601 gccttctgct actttcatac gtgctgtata aggaacagta tacgtacgtg tatctgtttt
      661 tcctttgatt gttacttctt tggtacgtgt tgcaggatct tctgagatat cgatgacatc
      721 ccctgttact tctgtgatca ctgcttgccc tttcggatta cgtgcttcaa agatttcttg
      781 gacacgagga agaccttgag tgatatcgtc tccggcaacc ccacctgtat ggaatgtacg
      841 catcgtcaac tgagtaccag gttcaccgat tgattgagcg gcgatcgtac caactgcttc
      901 tccgacttca acgtcagagc cagttgcaag gttgcgtccg taacaatgtt tgcagacacc
      961 atgttttgta ttacatgtga agacagaacg gattgtcact gtttcgacac ctgcatcaac
     1021 gatttgtttt gcgatgtctt cggaaatcaa ttggtttcct gcaatgatca attcatttgt
     1081 ttcaggatga cgtacttctt tacgggtgta acgtcccact aaacgatctt ctaatgattc
     1141 gatgatttca tttccctcgc ggatggcttg gatttcaaga cctcggtcag taccgcaatc
     1201 ttcttcacgg atgattacat cttgggccac gtcaaccaga cgacgagtca agtaacctga
     1261 atcggcagtc ttcaaggctg tatcggtcat ccctttacga gccccgtgag ttgagataaa
     1321 catttccaag acggatagtc cttcacggaa gttcgaaatg attggcaatt ccatgattcg
     1381 accattagga gcggccatca aaccacgcat tccggcaagc tgtgtaaagt tggaaatgtt
     1441 accacgagct ccagaatcag acatcatgaa gattggattt ttcgcttcca aaccttccat
     1501 cagtttttgt tggatgctgt cttttgcagc attccaaaca gcaatgacac gttcataacg
     1561 ttcgtcatct gtgatcagac cacgacggaa ttgttttgta atagtttcaa cttgtttatg
     1621 ggcattttca atgatttctt gtttttcatt cagtacactg atgtcagcga tccctactgt
     1681 gataccggca tgtgtcgaat gtttgtaccc taagtctttc atacggtcaa gcatcttaga
     1741 agtttctgtt actttgaatc gtttgaagac ttctgcgatg atgtttccta agtttttctt
     1801 cttgaacggt aatactaatt cttgctcttt gatatgtgca ggaatatcag taccagcttc
     1861 aacaaagtat ttgtccggtg tttggacggt caagttgaag tctgttggtt cattcagata
     1921 agggaattca ggaggcatga tctcgttgaa gatgatctta ccaactgttg tgatcatcgt
     1981 acgttctttt tgccattcag taaatggttt ttcacctaat gagttagggt tcacaccgat
     2041 acgtgaatgt aaatgaacat atccgttgcg ccatgcgata acggcttcgt tttgatctct
     2101 gaagaccatt ccttcgcctt cgcgtccttc ttcttccatt gtcaagtagt agttacctag
     2161 aaccatatct tgggaaggag taacaactgg tttaccatct ttagggttca agatgttttg
     2221 agcagctaac atcaacatac gtgcttctgc ttgtgcttct tcattcaatg gaacgtggac
     2281 agccatttgg tctccatcga aatcggcatt ataagcttca catacaagtg gatgcaaacg
     2341 gattgcgcgt ccttgtacta aaactggttc gaatgcttgg ataccaagtc ggtgaagcgt
     2401 aggtgcccgg ttcagcaata ctgggtgttc cttgattacg tcttctaaaa catcccatac
     2461 ttcgtcttct tgacgttcga ttttacgttt cgcatttttg atgtttgaag ctagttcgcg
     2521 ttgaacaagc tcacgcatca cgaatggttt gaataattca atggccattt cttttggaag
     2581 tccacattgg tacatcttca agaaaggtcc aacaacgata acggaacgac ctgagtagtc
     2641 gacacgttta cctagcaagt tttgacggaa acgaccttgt ttccctttca acatatgaga
     2701 aagagatttc aatgggcggt tacctggtcc agtaactgga cgtccgcgac gaccgttatc
     2761 gatcaatgca tctactgctt cttgcagcat acgtttttca ttttgaacga tgattccagg
     2821 tgcattcaag tctaacaaac gtttcaaacg gttattacgg ttgattacac gacggtaaag
     2881 atcattcaag tcacttgttg caaaacgtcc accttctaat tgaaccattg ggcgtaaatc
     2941 tggtggaata acagggataa cgtccataac catccagctt ggttgatttc ctgaagcgcg
     3001 gaatgcttct aaaatatcca aacggcggat tgcacgtgta cgtttttgtc cttgtgcaga
     3061 tttcaattct tctttcaatt cggctgcttc tgcatctaga tcaacattat caagtaattg
     3121 tttaatcgct tctgcaccca tagcagcttg gaattcttgt ccatattgtt cacgtttttc
     3181 gcgatattcg cgttctgtca acaattgttt tttctctaaa ttcgtatttc ctggttcgat
     3241 gactacatat gaagcaaaat aaatgatttc ttccaaagcg cgtgggctca tgtctaatac
     3301 aagacccata cgtgaaggaa tccctttaaa gtaccagata tgtgaaacag gagctgctaa
     3361 ttcaatatgt cccatgcgtt cacggcgaac tttcgaacga gtaacttcca ctccgcatcg
     3421 atcacaaacg attcctttat aacggatacg tttgtatttt ccgcaggcac attcccagtc
     3481 ttttgtagga ccaaaaatcc gctcacagaa caacccttcg cgttcaggtt ttaaggtacg
     3541 atagttaatc gtctcaggtt tttttacttc accgtaagac cagcttctga ttttttcggg
     3601 agaagccaaa cctatttgca tactttcgaa tttatttaca tcgatcaaaa ggggttccct
     3661 ccatttcatt taatggcaaa ccgcaagacg gctgatcttc gaccgccttg cagctcatag
     3721 accggattag tctaaattat cttctgcggt ttcaaagttt tggatgacat cgtctttttc
     3781 atcttccact tgttcagcag ctttcttttc taattctttg gcagtttgct gttcggcaaa
     3841 ttttgtcaaa gcatcaacag tgatcaaatc gtcgtcttca tcgtccatgt cgcgaagttc
     3901 gatctctgtt tcttcgatat ccaagacacg catgtccaga cctagtgatt gcaattcttt
     3961 caccaatacg cggaatgatt ccggtacacc tggttttgga attggttcac ccttgacgat
     4021 tgcttcgtat gttttcacac gtccgactac gtcatctgat ttgtatgtca agatttcttg
     4081 taaagtataa gccgcaccgt aagcttccag tgcccatact tccatttcac caaaacgttg
     4141 tccaccaaat tgagctttac ctcccaatgg ttgttgtgta accaatgagt aaggtccgat
     4201 tgaacgtgca tgcaatttgt catcgaccat gtgagcaagt ttgatcatgt acatcacacc
     4261 gacagacaca cgtccatcaa atggttcacc tgtacgtccg tcgtacagaa cagttttcgc
     4321 atcgctggcc ataccggctt cacgaactgt ttcccatacg tcttcatcac ttgcaccatc
     4381 aaatactggc gttgctacgt ggatacctaa ctgacgtgca gccataccca agtgtaattc
     4441 caatacttgc ccgatgttca tacgtgaagg cacccctaaa ggattcagca tgatatcgat
     4501 tggtgttcca tcaggtagga atggcatatc ttcttccggc ataatacggg atacaacccc
     4561 tttatttccg tgacgaccgg ccattttatc cccttcatgg attttacgtt tttgaacgat
     4621 ataaacacga actaacatgt ttacgcctgg tgacaattca tctccagctt cacgagtaaa
     4681 gattttcaca tcgtgaacga taccgccgcc gccatgtggt acacgtaatg atgtatcgcg
     4741 gacttcacga gctttttcgc caaagatcgc atgcaataga cgttcttctg cagataattc
     4801 tgttacccct tttggcgtga ctttccctac aagcaaatcg ccatcttgga cttctgcacc
     4861 aatacggatg atacccattt cgtctaaatc ttttaatgcg tcttccccga cattagggat
     4921 ttcgcgtgtg atttcttcag gtcctaattt tgtatcgcgg gcttctgatt catattcttc
     4981 aatatggata gaagtgtata cgtcatcttt taccaaacga cggctcatga tgatcgcatc
     5041 ttcatagttg tacccttccc aagtcatgaa accaactaaa acgttttggc ctagagccat
     5101 ttcgccttgt tccatagatg gtccgtctgc cagagtatcc cctttttcga ctttctcacc
     5161 caaatgaacg attggacgtt ggttgtagct tgttccggag tttgaacgac ggaattttgt
     5221 tacatcgtat ttatctaatg caccgttgtc gcggcgtacg cggatttcag aagcatcaac
     5281 gtattcaacg acaccgtcat gtttacataa caaagcagct cctgagtcat gcgctgattt
     5341 gtattccata ccagtaccaa cccatggtgc ttgaggattg atcaacggca ctgcttgacg
     5401 ctgcatgttg gcacccatca aggcacggtt ggagtcatcg ttttccaaga aaggaataca
     5461 tgctgtcgca acggcaacta cttgtttagg tgatacgtcc atgtagtcta ctttatcaat
     5521 agatacttct aagttttcac tttgagcacg agccataaca acgtcttgag caaatgttcc
     5581 atcttcattc aatgggctgt ttgcttgggc tacgatgtaa tggtcttcga tatcagctgt
     5641 caagtagtcg atttggtcag taacacgacc tgtttcacgg tctacacgac gataaggtgt
     5701 ttcgataaat ccgaatttat ttactttcgc ataactggac aaactattga tcaacccgat
     5761 gtttgggcct tcaggagttt cgatcggaca catacggcca tagtgggagt agtgaacgtc
     5821 acgaacttca taaccggcgc ggtcacgagt caatccgcca ggtcctaagg ctgaaagacg
     5881 acgtttgtgt gtcaattcgc caagagggtt tgtttgatcc atgaactgtg acaactgaga
     5941 agaaccaaag aattctttga tacttgccac aactggacgg atattgatca attgttgagg
     6001 tgtcaatgtt tcagtatctt ggatcgacat tctttcacga accacacgtt ccatacgtgc
     6061 tagcccgata cggaattggt tttgtaaaag ttcaccgact gaacggatac gacgatttcc
     6121 taagtggtcg atatcatcga cattgccgat accttccatc aagttcaaga aatagctcat
     6181 tgaagcaatg atgtcggctg gacgaaccgt ttttacagct gcttctggat agccgtttcc
     6241 gatcacgttt acttcacgtt cagggtctct tggtgaaaat actttgatga cttggacagt
     6301 cattggatct gtgaccacac cgtcttctga tggatagtaa gtcactgaat ttaggccatt
     6361 atcaatgaaa ggagctagtg tttccatgac ttggtgagtc agaactgttc ctttttcaac
     6421 gatgatctcg cctgtttctg gatcaactaa tgtttcagct aatgtcagat tcaatagacg
     6481 tgttttcaaa tctaattttt tgtttacttt gtaacgacca acgtttgcta agtcgtaacg
     6541 ttttggatcg aagaaacgag cattcagcaa gttacgtgag ctgtctgctg tttttggttc
     6601 gcctggacgc aaacgttcgt aaacgtcttt caaaccttct tctgttcttg agtcgcttgc
     6661 gtttttatgc aagtcttttt cgactgtatt gcgtaatgtt tcgctgtcgc cgaagatttc
     6721 aaagatcgta tcatcggaac caaatcctaa cgcacgaact aatacggtta aaggaatctt
     6781 acgtgtacgg tcgatacgta cataagagat gtcttttgca tcagtttcca tttctagcca
     6841 agctccacgg ttagggataa cagtagaacc gaatccttct tttccgtttt tgtcgacttt
     6901 tccatggaaa tagacacctg gtgaacggac taactgagaa acgattactc gctctgcccc
     6961 gttgatgatg aatgttcctt gttctgtcat taatgggaaa tcaccgaaga atacttcttg
     7021 tgctttgatt tcgccagtct cgcggtttgt caaacgcaat gttacatgta atggtgcaga
     7081 gtagtttgca tcatgcgcac gagcttctgc tactgtatac tttggttctt tcaattcata
     7141 atctacaaat tccaaagata gattgccatt gaagtcatcg attggcaaaa tgtcttcaaa
     7201 catttctcta agtccctcat ctaagaacca ttggtaagag tctgtttgaa tttcgatcaa
     7261 atttggtaat tccaacactt cactgattct tgcgaaactt ctacgttcgc gatgttttcc
     7321 gtattttact acgtgtccag ccaagctctt cacccctcta aaatgtcatt caaaaagact
     7381 acccgatcaa ttgttacaat aatatttcaa atattatttt taggtaacaa cagacatttt
     7441 ttgcggcttt taggcaaaaa aaaaccaaaa atagaacatt ctcttgaaac cttacttcgt
     7501 gagcttctac ttttgtttcc ttatattaaa gccgaaacgg acaatatttc gtttctgcct
     7561 tttcttcatc agatagtttt tgcaacttca tatactacac ttattttcta taaaagtcaa
     7621 cacttcctgg cgtttcttct gctttttcgc aacattttct taatgttttc aattttatgt
     7681 tttcaattat ctgacaattc ttaattgaca gaccttttta tctcggttat actttagaaa
     7741 attcattttg tggaggaact tctatgcgaa ccaacatcaa ccaaatcgca ccaacacaac
     7801 tgaataactt ttactttagc tactttagat aggtttgtat tcatcatgat ggatacaaac
     7861 gttaaacgac agtttgtatc tatgatggct aaagaacttt gtattgcagc aaaaagccac
     7921 gtagacaaaa tgctaagtgg ctgagcagtg aaaatacaaa gtaaaagtat caagcattct
     7981 actttttcaa tttattttcc gacttttcaa tccatttgga cattttgtct gaatggattt
     8041 tttgtttccc aaaattaagg aggaaaaaga aatgaaaaaa aatataatca tcggattagg
     8101 cgctgccctg ctactagctt taggaggttg cgcacaaaaa gacaattccg cagaggcatc
     8161 agatggacaa aaaacaattg gcgtattgca acttgtacaa cataactcgt tagatgctgc
     8221 ctataaagga ttcaaagaag gattagcaga agaagggtat aaagaaggcg ataatctaac
     8281 aatcgattat caaaatgccc aaaacaatca agacaacctg aaaagtatga gtgagaaact
     8341 cgtcaaagaa gatcctgatt tgctacttgg aatcgccact ccagcagcac aatctttatt
     8401 aaatgaaaca accactatcc cgatgactgt cacagcggtc acagacttgg aacaagccca
     8461 tttagtcaca tccaatgaaa aacctggcgg caatgtgact ggcacgacag atatggtgcc
     8521 aatcgaaaaa caaatcggct tactgttaaa tatcgtgcca gacgccaaaa caatcggcat
     8581 catgtataac aacggtgaag caaactctaa gatccaagct gatttggcag aaaaagaatt
     8641 gaaaaaagcg ggcgtcaaag tcaaagtact gacagcaaat tccacaaatg atgtccaaca
     8701 agtgacgaaa agtctagcta aagatgtaga tggaatctat attcctactg ataacacgtt
     8761 cgcaagtgcg gcaagtgttg tcggtgaagt cgcaaaagaa acaaaaacac ctgtcgttgc
     8821 tggcgcagtt gatcaagtca aagacggcgg tctagcaaca gtaggaatcg actacgaaag
     8881 cttgggtaaa caaacaggga tcatggcagc aaaaatctta gacggcaaga atccaagtga
     8941 acttcctgtc gaaacagcga atgatttgga actgtatgtc aataaagaca tggccaaagc
     9001 attaggcatc aatccagaca gcatcaaaga agctgaataa aacacaggag gattctcatg
     9061 acaaatttgc tctctctttt cttatcatct gcatcgcaag gggtcttatg ggccctactt
     9121 gcgattggtg tatacgtcac tttccgtatc ttggacattg ccgacttgac tgctgaagga
     9181 agctttcctt taggtgcagg gatcgcggca gtaagtatta caaatggcta tcatccattg
     9241 acagcttgtc ttctaggctt tttagggggt gcggcagcag ggcttgtttc cggactgctt
     9301 cacacaaaat tgaaaattcc cgcccttctt gcagggatca tcaccatgac tggtttatat
     9361 tctgtcacat cccgtgtgat gggtgcgccg aacatctctt tattgggtca gccaacgatc
     9421 tttcattggg tcgagtcatt cggcataaca aaagacaacg ctgtactgat cgtcggtctg
     9481 ctttttgctt tgatcgttgt atgtttactt gttttgttct tacgtacaga aacaggactt
     9541 gctattcgtg caactggcga taatctggca atgagcgaag caaacgggat caacacagat
     9601 aatatgaaga tcatcggtta tatgatttct aatggatgca ttgctctttc tggctccttg
     9661 cttgcgcaaa acaacggctt tgctgattta aattctggta ttggaacgat cgtaattgga
     9721 cttgcttcta ttatcatcgc agaagttttg ttacgtaacc agccactatt tctccgtctc
     9781 ttgactgtcg ttttaggtgc ggtcatttat cgttttattt tagcgttagt cttcgaactg
     9841 aatgttgaac caagtgattc gaaacttgct tctgctttcg tgctagtgat ctgtctttct
     9901 ttcccgcaga tccagcaaaa attccattta cctcaaaaaa agaaaggcgg tagtgtcaaa
     9961 tgaatgacgt cttaacattg gaaaatctcc atcatacctt tgaaaaagga acgatcaacg
    10021 aaaatcatgt cctgaaaggc attaatcttt ccttagatcc cggcgacttc gtgacgatca
    10081 tcggtggaaa tggggctggt aaatctactt tgctgaacag catcgccggt acattttctg
    10141 tcgatcaggg tcaaatttta ttgaatggaa aaaatatcac gaaaaaatct gttgtcgaac
    10201 gttcgaaatc catcagtcgc gtattccaag atcctaaact agggacagcc gttcgtttaa
    10261 cggtagaaga aaatcttgct cttgctatga aacgaggaaa aaaacgcggc ttcttccgtg
    10321 gtgtcaaacc acaagaccga acatttttca aagaccagct ggttcggcta aatttggggt
    10381 tggaaaatcg tttatcctca gaaatcggtt tgctgtctgg aggacaacgt caagctatta
    10441 cgttattgat ggccacatta cagcgtccag aactgatctt attagatgaa cacactgcag
    10501 cgcttgaccc tgctacttcc aaaacggtga tggaattgac agaaaagctg atcactgagc
    10561 agaatctcac tgcttttatg gtcactcatg acatggaaga cgcgatccgc tatgggagtc
    10621 gactgatcat gctgcatcaa ggaaggatcg tcgtagatat ccagcaagag gaaaaacagc
    10681 agctcacagt gccccaatta ttagcgttgt tcaaacaaaa cagcggcacg gcattaaaag
    10741 acgatcaagt acttttatct tcataatcag aaagaggatg ggacaaaaaa tgaacgccca
    10801 ccctcttctt ttttctcttt ccttctttcg tttttgcttt atgtatcaac cgttgtttct
    10861 tcgaaaacta ttttgttctc tccttcttcc tgatagaatg gatgggaaag gagcttgatg
    10921 ccatgacaac gaaagacaag gtacttcaat tattgaaaaa gacaccagat tttctttccg
    10981 gagaaaagtt ggcacagcaa ctggaagttt cccggacaag tatttggaaa gccatcaaag
    11041 aattggaaaa ggcaggctat cgttttgagc acgggcctac agggtatcgt tacctgccct
    11101 ctgacgtact agatgcaaaa gaaatccagc aagagctacg gtcgtttatt cctgatttga
    11161 aagtacaaac gatgttgact tctgaatcta cgatgaaaga tgccaaactg gcagcagctc
    11221 aaggagattc tgtttcgaag ttatttgttg ctgatacaca agaacaagct cgtggacgtt
    11281 ttggacggcc ttttttcgct gaacctggac gaggaatcta catgagtctc ttgctccatc
    11341 cagacaaacg gtttgatgaa ttgcctcaat atacgatcat cgcagccagt gcctgtgtga
    11401 aagccattga atctgtaacc ggtcgttctg cttcgatcaa atgggtcaat gatatctaca
    11461 tggatggaaa aaaagtctgt gggattttat ctgaagcagt cagtgatatg gaaagcggca
    11521 ggatcagtca tatcatcatt gggatcgggc ttaatttttc tgttcctcaa aatcattttc
    11581 cagctgatct ccaagaaaaa gccatctctg tctttcccga cggacaagct agtgtcacaa
    11641 ggaatcagct aatcagcaaa atctggcaag aattgttcac ttatctttct cacccagaaa
    11701 aagcgttgga cttctataaa gaaaaatcat tcgtattggg aaaaaccgtg tcgttcaatc
    11761 aaaaaggaac gacttatgtc ggtcttgcaa aagaaatcgg tgatcatggc gaactgatcg
    11821 tccaaacaaa tgaacagaag atgagtttgt cttctggcga aatcagtctc acatctatcc
    11881 gtacgcaata attatcagat ttcacaaaag gacacctgat ctcatacagt gtcctttttt
    11941 ctgaattctc ttcaaaagac tctccttatt ctatcagctg acagtttctc tatcctctag
    12001 aaaaaggtat tctaaaaata tctataaagc atggtacact ttctttaaat gaatcaatgc
    12061 aaaaaacaag agagtcatca gtttacacga tcaaaagaga tagcaggccg cttccttttt
    12121 caatcttata gatagaccat gggtaacgat aaaaacaaaa aagaacttgc gacttatttt
    12181 gccgtaagtt cttttttagc tagtgcctta atctcttaat cccaacaaac gtctcgcata
    12241 gatcacctga ccagagtgca tagcagcatc atcaataata gaaacgagac gcattcctcg
    12301 tgtgactggt ggtgtccagt tctcgtcaat tacttcatct agctcggaag gttccagcct
    12361 tcttaagtaa gaaatagcaa aatcagcagc tcctcgcaag tatcctagca gatcttctat
    12421 ttgattagct tggatctgct ttgcttgttc cagactatgc ttccaatctt gttcgttatc
    12481 tgctatcgtc atcggaaacc ggctttccca gtcttgacta tgccaaattg gagaagttcc
    12541 tgctaaatca gcaatctgga aatctaattc tcgagcggta tgccaagcca gccaagtcat
    12601 tgatttgatc tgcggggctc tatcttccac tggaaaacga ttggcctcct caatagagac
    12661 gccttcaaac aaacgttcaa accgatcgta tgcccgatcc acactctcgc atagcatatc
    12721 taaataatcc atttattttc ctccttttta tttatcctac ttactttttc ctaattctag
    12781 gatacctact tatatgttac tacacaccct tattttttac actttaacag tcaccatcat
    12841 caatagtttg gagattgaaa gaaaaagata gtgtcgaact atcaattgaa caagaagcaa
    12901 caatatgaac gtttcattaa tattctttaa ataaacaatg atttttcaaa cgaacagttt
    12961 aagactagga gaaaaagagt ataataggaa aatgtttgaa aggaaaggaa ggggaaaaat
    13021 gaaaaaaagg gcaagtttac tagtcctagt tgctttgttg ttcaatatct tttcgtatgg
    13081 tgtggccgtt tcagcggaaa catacattcc taaagaccct gggaatattt ataatgatct
    13141 aactggtaac tatggcttgt taggaattgc tagtcaattc cacgtatttg ctaaaaataa
    13201 gacaactgtg aatgcacata cagacggaaa tatagcaaca catgaacttg atgcaaaaaa
    13261 taattttggt acagagcttc atgaaggttt attgaaccaa gaaataaatt atatacaaaa
    13321 aactgattct ttcgctaatt cttcaggcat acctacaggt agcacaagaa tgaataaatt
    13381 tgtcgttggc agtaacgtgg atgtagcact tcaagatggt catcctacag tcaatggatc
    13441 caggcttgat cacttaactc cagatgattt ttataaagat cgtgctgggc atgaatatat
    13501 tgatttcgag caggaatttg ctaaattgaa tgtagcagca aatgatcttg caacgatcac
    13561 tccagctaaa acatatactg ctgcagattt tccagacatg aacaatcgga caatcgattt
    13621 aactgattta tcagataccg ggtttttatt ggtaaatatc gatgcagaag tgttaacgat
    13681 gaatacgcca cttcaaatca ttaatccaaa tgatcaagta gttgtattta atgtcatcaa
    13741 cagttcttct gcattgaacg tacagtcacc aatcaaatac aatgatcgtt caaatcatga
    13801 aacagaagat ttttcggatg cgaatatctc ttggaatttt ggaaatgaga tgactgattt
    13861 gacaatcagt gctccttttc aaggaacaat ccttgcgcct aatgctacta tccgcattac
    13921 tcaaaaccaa gacggaacga ttataggaac aaatgtgatt ttagatgcag ctaccaatcg
    13981 ttgggatcct aacgaaatat ttatcagcga caatggtaca gatacgacag acacaagcga
    14041 cagtacagat acatctacca caagtgatag ctcagattca tca
//
LOCUS       DACDUU010000074        13864 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.074, whole genome shotgun sequence.
ACCESSION   DACDUU010000074 DACDUU010000000
VERSION     DACDUU010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.074"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IWL30_002300"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IWL30_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAP9707030.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IWL30_002301"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IWL30_002301"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAP9707031.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="IWL30_002302"
     CDS             complement(2169..2906)
                     /locus_tag="IWL30_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP9707032.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="IWL30_002303"
     CDS             complement(2997..3524)
                     /locus_tag="IWL30_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAP9707033.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="IWL30_002304"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="IWL30_002304"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAP9707034.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="IWL30_002305"
     CDS             complement(4401..4886)
                     /locus_tag="IWL30_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAP9707035.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="IWL30_002306"
     CDS             complement(5039..5236)
                     /locus_tag="IWL30_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707036.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IWL30_002307"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IWL30_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAP9707037.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IWL30_002308"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IWL30_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAP9707038.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="IWL30_002309"
     CDS             9211..9423
                     /locus_tag="IWL30_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAP9707039.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="IWL30_002310"
     CDS             9425..11104
                     /locus_tag="IWL30_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP9707040.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(11358..11558)
                     /locus_tag="IWL30_002311"
     CDS             complement(11358..11558)
                     /locus_tag="IWL30_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9707041.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(11708..11869)
                     /locus_tag="IWL30_002312"
                     /pseudo
     CDS             complement(11708..11869)
                     /locus_tag="IWL30_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            complement(12249..12322)
                     /locus_tag="IWL30_002313"
     tRNA            complement(12249..12322)
                     /locus_tag="IWL30_002313"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12286..12288),aa:Ile,seq:cat)
     gene            complement(12634..13032)
                     /locus_tag="IWL30_002314"
     CDS             complement(12634..13032)
                     /locus_tag="IWL30_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707042.1"
                     /translation="MDKGNSVTFALENISLNKIHFELTDNFSYGDLEFNIELGYKEDE
                     AEYLEKKLVSLRAHIVGGMEGAPVDILLFSVIRVSTNEQPYKKLVEESMGELAKPILN
                     KASNLISNLYSEVGVMPTVIDLYQLYSQQK"
     gene            complement(13025..13477)
                     /locus_tag="IWL30_002315"
     CDS             complement(13025..13477)
                     /locus_tag="IWL30_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707043.1"
                     /translation="MKSINDLLKKHPDLYKKLNSEQRNLSKQLLKIRLKKQKNVTEFS
                     KMLGISEKKYLEFEFGDMHIPVAEYNKLLKQVENLSYKDSMNFTIKVAGGPIKYDRIE
                     NKPYIRNKSYSVFKSRKRKEVSSNMVYASVNYQDTSRYIVEKEVYVHG"
     gene            complement(13464..13757)
                     /locus_tag="IWL30_002316"
     CDS             complement(13464..13757)
                     /locus_tag="IWL30_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707044.1"
                     /translation="MGILQRSEIVKELFYHKFSYEIRLDDTYEHYRIVFFVYNSIEDA
                     TATTALTFGFTKNGTINSDKTRQAATESDGICKKVCNGEENYWIGEEKLNEIY"
BASE COUNT         4081 a         2920 c         2337 g         4526 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaatacctgc cacaagagaa attgtctctt
    11281 gtggcaggta tttttgttac tcgctttgtt ctctcgtaaa aattgatgtc taccatcaat
    11341 ttgatttaca gataaaatta agcttttaca acgtttgcag cttgtgggcc gcggtctgat
    11401 tcttcaacgt cgaaagtcac tgcttgacct tcttctaatg ttttgaatcc gtcaccttgg
    11461 atagctgaga agtgaacgaa tacgtcgctt ccatcttcac gtgagataaa tccaaatcct
    11521 ttttcagcgt tgaaccattt tactgttccg ttttccataa taatatcctc ctcgtgccaa
    11581 aagcactaat ttaaattgta acattgctcg tgtacgatag aggtgcaaca gatgtttcaa
    11641 cttctattcc caaacaaaat tacaatataa ttgtacactt gcttcatttt ttttgcaaga
    11701 aagcaactta cttctcttcc atcattcctt cttctctcaa actaaaatat cgtttgcgtc
    11761 cgatgatcaa gtggtccagt acagtgatcc ccatcatttc tccacacttt tgtatccttt
    11821 tggtaaaatt catatcctgc tgcgatggaa taacattacc agaaggataa tataggttag
    11881 tacacgtata aaaatatctt ttatattgac acaatttatt taacaagtat aataataatt
    11941 gaaagtgaat ttaaacaaat atacaccata aacacaacgt acctgcgaat acgttgtgtt
    12001 tttttctttt tcaaaaaaag acgcttaccg ctgcgaaaca gtaagcgcca ttgtcggctc
    12061 taatgtttat ctagccaatg actaaataat gccaaagtta aaccaattaa caatggcaag
    12121 agcacgtgct ccaaagtgaa ttcaaacaaa tcatacacct cctcccacga aaatctctgt
    12181 ggaagcgacg agccgatatt ttattatacc attattgaat tttatttttt atcaaataga
    12241 ttaaattatg taccctgtag gactcgaacc tacgaccgga cggttatgag ccgtctgctc
    12301 tgaccaacta agctaagggt actggttgtt gccacataaa gccataaaca atcaaccagt
    12361 agaatgtgtg gcaacaaacc tgttatcgca tagcttggag tgtgactatt atgggtgata
    12421 gtgaagatat gcgataacat cactatttta tcgaatgatt tttatagttg tcaatacagt
    12481 catctaaata cttcatatca atcatttatt cagaaacaaa ctatcttgca ggatctatac
    12541 gagttaatta cagagcaaaa aagcctcaac caaggatgac tggttgaggt ctttgtcttg
    12601 caaatttaaa tagataactc tattacttgt attttatttt tgttgactat atagctgata
    12661 taaatcaata actgtaggca taactcctac ttcgctatat aaattagaaa tcaaattaga
    12721 agctttattc aggataggtt tagctagctc tcccatagat tcttctacta attttttata
    12781 tggctgttca tttgtagaaa cacgaataac tgaaaagagt aaaatatcta ccggtgctcc
    12841 ttccatcccc cctacaatgt gagctcgtaa agaaacaagt tttttttcta gatactctgc
    12901 ttcgtcttct ttatatccaa gttcaatatt aaactctaaa tctccataac taaaattatc
    12961 agttaattca aaatgaattt tattcaaact aatattttca agtgcaaaag taacactatt
    13021 tcctttatcc atgtacatat acctcttttt ctacaatata gcggctcgta tcttggtaat
    13081 taacactagc atagaccata tttgacgata cttcttttct ctttctactt ttaaatactg
    13141 agtaactttt attccgaata taaggtttat tttcaatacg gtcatattta atagggccgc
    13201 cagcaacctt aatagtgaaa ttcattgaat ctttatagga caaattttca acttgtttca
    13261 aaagtttatt gtactctgca acaggaatat gcatatctcc gaactcaaat tcaagatatt
    13321 ttttttcact tattcctaac atttttgaga attctgtcac gtttttttgt ttttttaatc
    13381 ttattttaag taattgtttg gacaggtttc tttgttcaga atttaatttt ttatataaat
    13441 caggatgttt ttttaataaa tcattaatag atttcattca acttttcctc ccctatccag
    13501 taattctctt ccccattgca tactttctta catataccat cactttctgt agcagcttga
    13561 cgggttttat ccgaattgat tgttccattt ttcgtaaaac cgaatgttaa tgcagtagtc
    13621 gcagtagcat cttctataga attgtatacg aaaaaaacaa ttctataatg ctcataagta
    13681 tcatctagtc ttatttcata actaaattta tgataaaaaa gttcttttac tatttccgat
    13741 ctttggagta ttcccatccg atttcttaag cttagctcaa taggtgtttt tattggagaa
    13801 tcattttcac caattaattt atttttttta acagagtaaa tttcaagata ttttaataga
    13861 agga
//
LOCUS       DACDUU010000075        13509 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.075, whole genome shotgun sequence.
ACCESSION   DACDUU010000075 DACDUU010000000
VERSION     DACDUU010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.075"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(92..634)
                     /locus_tag="IWL30_002317"
     CDS             complement(92..634)
                     /locus_tag="IWL30_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAP9707045.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            769..1158
                     /locus_tag="IWL30_002318"
     CDS             769..1158
                     /locus_tag="IWL30_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707046.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1162..1926
                     /locus_tag="IWL30_002319"
     CDS             1162..1926
                     /locus_tag="IWL30_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9707047.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2055..3002
                     /locus_tag="IWL30_002320"
     CDS             2055..3002
                     /locus_tag="IWL30_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9707048.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3132..3872
                     /locus_tag="IWL30_002321"
     CDS             3132..3872
                     /locus_tag="IWL30_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707049.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(4163..4831)
                     /locus_tag="IWL30_002322"
     CDS             complement(4163..4831)
                     /locus_tag="IWL30_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9707050.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            4965..5252
                     /locus_tag="IWL30_002323"
     CDS             4965..5252
                     /locus_tag="IWL30_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707051.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            5668..7206
                     /locus_tag="IWL30_002324"
     CDS             5668..7206
                     /locus_tag="IWL30_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAP9707052.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            7203..8168
                     /locus_tag="IWL30_002325"
     CDS             7203..8168
                     /locus_tag="IWL30_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9707053.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            8155..9477
                     /locus_tag="IWL30_002326"
     CDS             8155..9477
                     /locus_tag="IWL30_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9707054.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      9540..9644
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            9718..10461
                     /locus_tag="IWL30_002327"
     CDS             9718..10461
                     /locus_tag="IWL30_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9707055.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            10476..11468
                     /locus_tag="IWL30_002328"
     CDS             10476..11468
                     /locus_tag="IWL30_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9707056.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            11480..12217
                     /locus_tag="IWL30_002329"
     CDS             11480..12217
                     /locus_tag="IWL30_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9707057.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            12207..12929
                     /locus_tag="IWL30_002330"
     CDS             12207..12929
                     /locus_tag="IWL30_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP9707058.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(13013..>13509)
                     /locus_tag="IWL30_002331"
     CDS             complement(13013..>13509)
                     /locus_tag="IWL30_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAP9707059.1"
                     /translation="QKYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAE
                     PQAAVQEAPVQAEQPVVQETVQTEKQAAPVAETQPAPAVTETAATPASTSSAKEWIAQ
                     KESSGSYTATNGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEA
                     NGWY"
BASE COUNT         4452 a         2306 c         2804 g         3947 t
ORIGIN      
        1 tatgtcgatg ttttttaatc aaaataataa ataaaaactg tctaacattt aaaatttaat
       61 ttagatatta gacagttttt aagtttgtga gttattttcc ttgtcgtttt ctcacttttt
      121 tagtccagaa accacctgaa atatattttg gctcataaga aatgataaaa gcacgctctt
      181 cgatttcttt gatcaggcga tacagttcac gttcgctttt tctaggagaa agtatttcga
      241 gaactagccg ctcaccttcc agccccattg cagaagtctg tgtcacacca taaccgtgtt
      301 ctctcagtat acccggcaaa cttttatctt ctgcgctatt tgctggcaaa atcgttgtga
      361 ccatgatgta tcctaatgcc aatttgtctt caatcaaaat tccgacactg atacctactg
      421 cgtaacctaa cgcatagaca aataaattca gtggattatc taaacgatct aaaacaaggg
      481 ataagccgac gatataaatc gtgatctcga ccatactgac aagtggagca atcagtcgat
      541 agcctttcat tgtcaacatg aaacgtaacg tgttcagagt gatataagca aaattaatga
      601 ggaagatcat ccccaacatt tttggatcaa tcatttgtaa aactcctttc atttatataa
      661 caacaatatc aaaaaaaatc gtaagtgaag aaaaattgaa actttataca tacaaaaaaa
      721 tgataaaatg attataatag aattataaag ataggttagg tgattaaaat gaaaacggtc
      781 aatcgtgtgt tttgtgccag ccacgtgcta agtctccttc tcagtatata cttaggtgtc
      841 tattcgattc aacagccaat tgaggtagag aacattgttt ttcagatgtc gctgacagat
      901 ggtttaattt taagcgtttt tttctttttg atactgttta ttggtaatat tttcggtagc
      961 gttgccagtt ttttgaattt ttctatttat ccactccttt cgctagcatt aggagtggtg
     1021 ggactttgtt ctctgtgctt atttcccgag ccattcagtc ctgcatttat attatttggt
     1081 acactgaacc tgttccagac aactgttgga gcatggttgc tatggcgatc aggaaattta
     1141 atgaaaatag gtgagtaaca attgaatcca attatattga aacgttctgt ccatcatatt
     1201 ccagtgttag aagttgttct gaaagaatta agagaggaac gtattcccgt tgtcatctat
     1261 tatcacggat ggcagacaag taaggagtta gttttgacgc aaggaagaaa aattgcgcag
     1321 cagggcattc gtgtgatcct gccagatgcg atgaatcatg gggagcgcaa acagccggtt
     1381 tcttctattc catcctatac attttgggat agtatctacg gaaatctgtt tgaatttgac
     1441 acacttatca agcatttaga aaaaagaggt cttctacatg atcagcttgc agttggcggg
     1501 gtttcgatgg ggggaatgac aacatgcgcc ttgttggcta aacatcctca tatagtagcg
     1561 ggcgtgtgtt tgatgggttc ccctgcacct ttggaatatg gaaaagaaat cgtaaggcgc
     1621 gcatcggaat ataattatgt cgtaccaaaa gactattttg atttgatttc atgggttaag
     1681 agctatgatt tgtcattgca gccggaaaag ttagctggac gtcctctgtt cttttggcat
     1741 ggacaagctg acgaaaaaat cccttatata caaacggaac gatttgtaga aaaaaatcgg
     1801 cataagtctt acggaaaaga aattgttttt cgttcctcaa aaacacagcg acatatggta
     1861 caaataccac taatggaaga agcttccact tttttagcaa aaaatttgat aaaaaagtat
     1921 aaataattga aacggaaaat cactcatgac gatagaggat tttccgtttt ttattttggg
     1981 ctataataaa ctctgttagg aaaatttggt ataattgata gtaagaatga caacatgtta
     2041 gaaaggaaga gagcatgccc actttagcag atgttgcaaa acgtgccaat gtttcaaaaa
     2101 tgacggtctc tcgagtcatc aatcatccgg aacaggtgac tgacgaattg aaagagttag
     2161 ttttctcagc aatggctgaa ctggattacc gtccaaatat tgctgcaaaa gcacttgtca
     2221 cgaatcggtc gcagatcatc aaattattta tactggaaga aatcgacaca actgagccct
     2281 attacatgaa tttgctgatg ggaatcgcta aacggatcgg taaagcgcat tattctctcc
     2341 aattagttac aaatgatgcc ttcgacgtcg gttcttgtga tggatacatc atcacaggtg
     2401 tcagaaaaaa agacttcgat tggatcgacc gtttggaaaa accagttgtc ttgtttgggg
     2461 aaaattctga aggatatgat tatgtagaca gcgataatga atatggtaca gcaaaagcaa
     2521 cgcaatatgc cttagaaaga gattatcaaa cgattattta tatcggtatc gaagtggacg
     2581 agccttttga aatttcaagg gaaactggtt atttatctgt gatgaaggaa gttgatcttc
     2641 ctagtaaaat cttgcggttt ccaaatcatt cgacagaagt agaaagatat atgacagaaa
     2701 actgggcaga ttttacagat aaaacttgtt ttgtatgtag ttcagatcgg ctagcactag
     2761 gagcagtaag agggattata aatgctggag gacacttgcc cgaagaattt ggtgtgattg
     2821 gatttgatgg ggtattttta gaccaaatca gttcacctaa attgacaacg atcaagcaag
     2881 atattatacg tatgggtgaa gtatgtggag aaatgttgtt aaaaaaaata gaagaaaaag
     2941 gggcgagtca aggttatcgt cgtttttttc ctgaactagt tatcagagag acaacgaatt
     3001 aagtcttatt actatatata tatttatgaa attcatcgct attttcttaa aataatcagc
     3061 gcattgctac tgaaaacaat gaatctgtgc tattattgag aaaagttcca tttttgagaa
     3121 ggatggtttg aatgaaagaa atcagacgaa aacatttccg ttttcctgcg tacgatgatg
     3181 agttaggcgt aaaattaacc aacgacaaga agcgagtatt atttgacgga caagaagatc
     3241 ttttgactga acagaatcag tccccgtttg aacagatgaa agaatttcat ttcacgaaca
     3301 cgaacaccga gaaaatccgt cgcaagcgtc gtgcaacaaa tggattgaag tcagctccgc
     3361 gtaaggaaaa tttggaaaag cataaaggaa aattaccgga ttataccggc cattaccaat
     3421 cagaaatcac atcgacagga cgaaaaaaac tttttggtga ttcctctcgt tctgtcagac
     3481 aaagtgaaac aaggacagag tccaagcata ctacacgaaa cgcatctgtt gacacgaagg
     3541 tgcgggagcg ttcttatttt gttcctaaat atgtacccgc atcgattatc ccagacccta
     3601 gagaaagcaa aatttctgag gatcatttaa tcgaatcgat gaaaaaaacg gatgactctt
     3661 atctgttgtt cgatacagaa ccgacgccgt atcaagaaaa aagaaatggt gagccgtctg
     3721 tacgaacatt caatcgaaca aaaacgttag aggaaacaaa gcagtcagaa gaaaaaaaat
     3781 cgaaaaaaag ccatggtatc ttagaacgct cattacaagg attgatcgat gatcaaggaa
     3841 actctttggg agaaaatagc tattttaaat aagtgatata caaatttata ttcggctaca
     3901 gttggaaagg aaacgctgcg gcgtttcctt attttatact ctatccgaaa gtttatatcg
     3961 tatcctcaaa tttttttgag tttaagcgaa ttttttctcc caataagtaa gaaaataatg
     4021 gcaaaataaa aaaacaaact attttcgcag atcgtttgca aaaatagttt atttttttat
     4081 aaagaagatt ttactgaata atatcatttt agtttacata atgatatttc tgtaaattaa
     4141 ataaagtagt gaaaaataag aatcagtgct ctaactgttc aagaggatca agtggttcaa
     4201 atgtttctgt agtctgtttt gcttccaatt ggggcaaaac agatccaatt tccagatact
     4261 tcactttatt caatgtataa gatagaatag aacagctaag taatgtatac agaaaatgtg
     4321 tgagatccaa ataaagcaag gagacaacta aaataccaaa agtaatcaag aaaagacttt
     4381 tgccaatctt aatatgaaaa tatttttcaa tggcttgata aatgatatct gttccgcctg
     4441 tgcttccgcc gaatcgaaag acgagaccgc aaccaatgcc agaaagaata cctgtcatca
     4501 aactattgat cagtagacta tctacagcaa aatgtatatg aaggctctcc cataaatgta
     4561 aaaagaaaac tagcgcaaat gttccgacga tcgtccggag aaaagcgtgt tttccaaaaa
     4621 gaaacaatga gataagcaat aaaggtaaat taaaaagcag tgaagtgaca gaaggagcga
     4681 atccaaaaag attatgcaaa agcaaagcaa taccagtaac cccaccatct gccagcttag
     4741 atggaatatt gattcctgtc acagctaatc cgtagattcc tgcgcctaaa atgattccgc
     4801 acaattctgt aaataaattt aattttttca tttctagcct cttttctttt gaaaattttc
     4861 agcaacttct ttattatact gacttatact cttacttcaa gaggaaaatg aaaaaaaatt
     4921 atttttggct gtttcttttt atttatcaaa taaggagtgg aaaaatgcga gcagcagact
     4981 tattgacaat tcttacaaaa tcacccaaaa aaacagagta tcaaggagtt tttttggctc
     5041 aagaacaaaa actggtgcct gtgacaatgg tccagataaa taaagcaaat caatttatct
     5101 ttttatttga acacggcaaa gcaccacttg ctatgaaaga catctttctt tttttgatga
     5161 agaaccgtga gaaagctgtt tattatcagt caaaagaaga actcattcct ctttatggag
     5221 tgaaagagat agaaaataaa gtggtgatat aagtaaaaat aagaaaacaa gacgtttatg
     5281 atgtcaaaac aaccattttt tacatttttg gcaaaaaagt gtcatgatac agtataatat
     5341 tcgttgaaca ggccaataaa ggtgttctcc cccacgagat ttctacttta ttggctgttt
     5401 ctttttatta aaataaaagt tgtgtgtgta aaaaagatat agctgattcc aagtgacaat
     5461 ttcgtataga tttttatggt attcgtagtg aaaaaacgag aaccaaaaag ttgatccgac
     5521 tttgttgctt ggattctttt tttgtcagca tatttagagg tatttttttc ttttttttgt
     5581 tcgtgctgta aggttaaggc aagacaagtt agttgggaaa aaggaaagaa atgtttatac
     5641 ttaaagaata acagaaagga aggtcgcatg aataatttta tacaaacatt aagtgaaaga
     5701 aaaggcgagt tgctggaagc tgcatttcaa catctgtcca tttctctcat ttctttgctg
     5761 attgcagcac tgattgctat tcctttagcg atttgggcag ccaatcataa aaaaatggca
     5821 gaagtcttat tgcagatcac gggtgtgctc caaacgattc cttcacttgc cttattaggt
     5881 ttacttatcc catttgtagg gatcggaact gttccagcat tgattgcact tgttatttac
     5941 gctttgcttc cgattttcca aaacacgtat attggtctag ctgaaattga cccgtctatt
     6001 gaagaagcag cggtagcatt tggtatgtcc agaatgagac gcttgctgaa agtagagctt
     6061 cccatcgcat tacctgtcat tatctcaggt atccgaactg ccttagtttt aatcatcggt
     6121 acggctactt tggcagcatt gatcggagca ggtggattag ggacatttat tttgttaggt
     6181 attgaccgaa atacacctgt tttgacattg attggtgcga tcagttctgc cttgcttgca
     6241 attatattca gcggactgat tcgctggttg cagcataaga aacctcgata ttccatcatt
     6301 actttagcag cagtggtgtg tttatttggc ggcgtttctt tatatcagtc tgccttgttt
     6361 aaaaatgaaa ccatcacgat tgctggaaaa ttaggagcgg aacctgaaat cctgatccaa
     6421 atgtataaag aattgattga agacaacaca aaaacagaag ttgaattaaa accgaatttt
     6481 ggcaagacga gttttttgtt caatgcactg gatcaaggtc aaatcgatat ctatcctgag
     6541 ttcacaggta ctgtcctaga aagcttggtg aaggtacctg aagcaatgaa aagtgctgat
     6601 ttgacaaaag aaaaaacata tgacgaagca gaagaattat tgaataagca attcgatatg
     6661 acattattaa aaccaatggc ttaccaaaat acgtatgcct tggcagtcaa gagatctttt
     6721 gcgaacgaac atcaacttaa aaagatttcc gatttggcag ctatctctga tcagatcaaa
     6781 gcgggattca cactggaatt tattgatcgt tcagatggat ataaaggaat ccaagaagca
     6841 tatggcttga atttttcaag tgtacaaagc atggagccaa gtttgcgtta ccaagcaatc
     6901 aataatggag atgtcaatat tgtggatgcc tattccacag atagccagct caaggagtac
     6961 gatctagtga ctctagaaga tgatcgaggg ttattcccag cttatcaagg cgcaccgctg
     7021 atgaaaaaag attttgctaa agaacatcct gaaatcgtta aagcactgaa taaattaagc
     7081 ggaaaaataa cagaggatca aatgattgat atgaattacc aagtcaatgt cgaaaagaaa
     7141 aagccgacag aagtagcacg aatcttcctg aaaaatgaag gattagtaaa ggaggaagat
     7201 cagtgactaa attaatcgag ttcaaacatg tgcaaaaaaa gtatgatgga aagtatgtca
     7261 tcgatgacct aaatctggca atcaaaaaag gagaaatctt tgttttggtt ggtccttccg
     7321 gtagtggaaa aacaactact ttgaaaatga tcaatggatt atctaaacca tcagcaggag
     7381 acatctactt caaaggaaaa tcgttaaatg aatataatct gcagaaaatg cggtggaata
     7441 tgggatatgt gctgcagcaa atcgctttgt tcccgaccat gacagtcaag caaaatattg
     7501 aagtcatccc tgaaatgtta ggttgggaaa agcagaaaag agcagaccgt gtagatgaac
     7561 ttcttcaaaa agttggctta tctcctgata tttatcgaga tcgtatgcct agagagttgt
     7621 ctggaggaga acagcaacgg atcgggatca tccgagctat tgctgcaagc ccagatgtga
     7681 ttttgatgga cgagccattt agcgcattag atccgatttc aagaaactca ttacaagaat
     7741 tggttttgtc cttacacgaa gaattgggca cgaccatcgt tttcgttacg cacaatatgg
     7801 aagaagcaat caagctaggt gatcgtattg catttatgaa ggatggagaa attatccaat
     7861 gtgacacacc agagcagtta ttgatgaatc ctaaaaacga ctacgttcgg catttctttg
     7921 atgaaccgaa acagacgaaa gagtggcgag tagaagattt agtcgtcaat ggatatttct
     7981 taaatgaaat accagaaaat gctcgtcaac aagtttgctt tgatacgcct atgaaagaag
     8041 tatattcgct tctttcggtg tatccaagca ttgtgatagt agaaaagcaa agaagtattg
     8101 ggtcgctgac aagtaaagaa atctttgctt ttttaagtcg agaggaggaa ggaaatgaaa
     8161 acatatgatg caatcatcat cggttcaggt gtgagcggct taagtgctgc ttatggattg
     8221 aaagaagcag gtaaaacagt actagtggtc gaagaagatc tatggggcgg gacttgtcct
     8281 aaccgtggat gtgatcctaa aaaagtcttg ttaagtgcag tagaggcacg taatcgggta
     8341 aaacaattga gtggaaaagg ttttaacgag atcccaactg ccaactggga agaacttcaa
     8401 aaattcaaac gaacatttac ggatccagtc ccagaaagcc gaaaaaaaca attagcagaa
     8461 gctgaaatcg atcatctttc aggaaccgct cgttttttgg atgattcatc tattgaagtc
     8521 aacgaagaag tcttccatgc ggattactta gttttggcaa ccgggcaaag acctacgatc
     8581 ttaccagtgg agggaaagga atacttgaaa actagtgcag actttttatc attgcctgta
     8641 ctgcctaaag aaatcatttt tatcggtggt ggttatattg cttttgagct agctacgatt
     8701 gccaatgctg cagggagcaa agtaaccatt gtccatcata atcaacggcc attaaaagaa
     8761 tttgaggcgt ctttggtaga ggaagccgtc catcaaatgg aagcatctgg cattcagttt
     8821 gcttttggtg ttgaaaccca aaagatcatt tcagaaggta ctcgctatcg tttagtcgga
     8881 aaagagacgg aattagtagc agatatgatt ttttgtgcga ctgggagaca gccgaataca
     8941 gaatctttag cacttgagca agcgaatatc gtctttgata aacacggaat tgcagtcaat
     9001 gattatctgc aaacgagcaa tccaaaaatt tttgcgtgtg gagatattgt ttcaaggaaa
     9061 accccaaaat tgacaccagt cgccactttt gaaggaaatt atgtagctaa gcggataacg
     9121 gatgcaacaa gcgaacctat caaatatccg attataccta cgatcgtcta cgccagtcct
     9181 aaacttgctg aagtaggagt tacaaaaagt cacgcctctt cgtcagatca agtggtagag
     9241 atggatctta ccagttggtt cacctatcat cgtgtcaatg aaccagttgc taaggctgaa
     9301 ttgacctttg accagcaaaa ttatttgatt ggtgctgcag tgattagcga gcaagcagac
     9361 gaattgatcg atgatttgac actcgtcatc aatcaaaaat tgactaaaaa agagttggat
     9421 agctatatca tggggtatcc gacattggca agtgatctct cctatttatt gaaataatca
     9481 gcatgataac taaagcgctt gttgcttttt ccttcttgtg ctacacttta aaaaatgaat
     9541 acctgtttct ggggatgtcg tagacgacat gagagtaagt tgtgaactta gacccattga
     9601 acctgtcagt taatactggt gaagggagaa acatagcaga cgcttgaagc gggttactat
     9661 gtcgtgccca tttcaagcgt tttttgttat tcatattaga agaaaggagg taacaaaatg
     9721 aagaaaaatc tttatccagc agtcagtatg gcattcattt taattatttg gcagattgtt
     9781 gtcatagttt ggaaaatacc tgcctttatt ttacccggac ctgctgctgt attttctgca
     9841 ttagcggcag atgcacaagt attactttcc cattctgtca cgacattaaa tgaagcagtg
     9901 cttggtttgg taatagctag cgttctttct tttattactg ccttggcaat ggattattgg
     9961 aagtttttag aaatgtcttt ttatccgtta ctagtgattt cacaaatgtt gccaatcatg
    10021 gtgttagggc cattacttac cctatggttt ggtttcggta tgcttccaaa agtgatcctt
    10081 gttgtcttga taagctattt tccgatcgta gtttcttttt cggatacatt gaggaaagta
    10141 tcaaaagagc aaatcatctt tttgaaaacg atgggggcag atacagggaa gatttatcga
    10201 atatataaaa taccagcggg tttatccggc ttcttttccg gtttaaaagt ggctgctact
    10261 tattgtgtca gcggagcagt cgttggagaa tggctaagcg ctcaagcagg cttagggtat
    10321 tacatgatac gcgtaaaaaa cagctaccag atcgataaag tttttgctgc gatcatttgt
    10381 gtcattctat tgagtttatt gctgaatggc gcttgttcat tattgaaaaa agggtattat
    10441 cgtatgcttt atatttttta agagaagggg atcaaatgaa aaaattatgg cttttattac
    10501 cgctactgtt gcttttatcc gcttgtggca ctgcaaaaga aacaagtagc aaacaagaaa
    10561 taaaggattt gaaaaaagtg acacttgttt tggattatgt accgaatact aatcatactg
    10621 gcatctacct agcaaaagaa aaaggctatt ataaagaggc tggtttaaat gtccaaatca
    10681 tcgaaccagg agataattcg acaagtattg gtctagtagg tgcggacaaa gcacaatttg
    10741 gtgtaagtta tcaagaagat gtcacttatg ctcacgcaga tggacagaat ataccagtca
    10801 aagcaattgc tacagtgatc aaacataata cttctggatt cgccacccta tcagacagta
    10861 acatccattc accaaaagat tttgaaggaa aaacatatgc aggttggcaa agtccaagtg
    10921 aagaagctgt cttaaaagct gtgatggaaa aagacggcgg ggacttttca aaactgacaa
    10981 tggtaggaag caatggagaa gggccagaaa gcttaggaaa atcctcagat atccaatggt
    11041 actttgaagg ttgggatatg atcaaagcaa aagaagcggg tattgaacta aactatatcc
    11101 cattgaaaga attagatgaa cggttagatt actatactcc ggtaattatt acgaatgatc
    11161 aactgatcaa aagtgatcca gaattggtac agtcttttat ggatgccacg aaaaaaggct
    11221 atcaggaagc tattaaggat ccaaatgaca gtgctaaact gttgcaaaaa tatgcaaaag
    11281 aaaatgatcg gacgttttta gaagaatcac aagcattctt gtcaaaaaat tatacggatg
    11341 atcctgagaa ttggggattg atggaagaaa aagtttggcg caattataca gcttttatgc
    11401 aggaaaacgg attgatcaag caagatgtac cttcgcaaga actattcact aatcagttta
    11461 taaaatagga gtgatagata tgtcaatcca aatcagccac ttgaatgtag tcattgaaaa
    11521 cctgaaaata ttagaagaca tccatttcac tatccctaaa gataccttta catcgattgt
    11581 cgcgccaagc ggagccggta agtcaacatt gctgaaaact ttaacaggag tccagcctat
    11641 gacttctgga acgatcaaag tagacgatca aaaagtaact ggtctccata cagcatttag
    11701 ttatatgccg caagaagata tgttacttcc ttggctgagc gtctatcaaa acgttacgtt
    11761 atatcaaaaa atcaatcatc taacaatcaa tgaagaacaa gtaagggatt atctggatat
    11821 ctttgggtta aacggctatg aacatttttt gccggaacaa ttatctggcg ggatgaagca
    11881 gcgaacggcc ttgttacgaa caatcatgaa tccttcttct tacttgcttc ttgatgaacc
    11941 atttggagcg ttagatgcta tgaccagagg gcagatgcaa gactggatgt tgaagctgcc
    12001 taaaaaagca aagcggacta ctttacttgt gacgcatgat atcgaagaag caatttattt
    12061 atcagacagg attcttgtgc tttccgcccg tccggcacat gtgattgctg agattcaagt
    12121 gcctgaaaaa cgacggtcac gtgaatggct actgcagcaa tcagagttga agcaaacgat
    12181 ttatcagtta ttggcaggtg aattagatgt taagtgatgc tcattgtcat ttagatggga
    12241 gtcagagttt agcactcctt cagcaggaac acacgatttt gacgatcatc aattgtgatt
    12301 cgccagaaga atggaaggaa aacagacagc ttgcagcaag taagactcaa gccttaagtt
    12361 acggtatcca tccttggaaa gcagatagct atacgtttga acaagttgag ccttttttga
    12421 aaaaagcaag gatcatcggt gaaatagggt tagataatat ttggacgaat gtaccgatga
    12481 ctacacaaaa aaaagtattt gagcgacagc ttgcttttgc agccataaat gagaaacctg
    12541 ttgtactgca cacaaaagga tgcgaaaaag agatcttaaa ttatatccaa gcttacccta
    12601 atcactattt gatccattgg tattcttctt tggactatca acaagactat attgatttag
    12661 gctgttattt cagcattggt cttgatttga aaaagaatcc agcagtctgg caactagcta
    12721 aagcagtccc aatcgaccac ttacttattg aaacggatgg actacatgca gcagagtggg
    12781 ctttaggggg gattttttca gaaaaagatt atgtctcact gcttgaagaa cagttaaaga
    12841 caatcgctga gatcaaagag ctatcagttg aagaagttaa aaaaaatact tatcaaaact
    12901 tggaacgttt cattagatta ggtaaataaa aaagagatgt gacacaacta gtgtcgcacc
    12961 tctttttggc tttctttgta taaaagtaaa ctgtttgatt aataaatgaa tcttagtacc
    13021 aaccgtttgc ttcccagaat gctttcgccg cttcccaaga accataacgt gaagtaacat
    13081 attgttctgc tactctttct tggtttgcag cagagtagtc accatttaag taagaagaat
    13141 ctaattggta acgtccgatg taacgtccgt tcgttgctgt gtaagaaccg cttgattctt
    13201 tttgtgcgat ccattctttt gcagagcttg tgcttgctgg agtagcagca gtttctgtta
    13261 cagcaggagc aggttgtgtt tcagcaacag gcgctgcttg tttttccgtt tgaaccgttt
    13321 cttgtacaac aggttgttca gcttggactg gtgcttcttg aacggctgct tgaggttcag
    13381 cagctgcttg tgcagagcct tctgttggga ttgttaattg ttgtcctgag taaatcaggt
    13441 taatatcaga aattgaattt gattctgcaa tggcattgat caaggagtta tcaccaacgt
    13501 atttttgag
//
LOCUS       DACDUU010000076        13429 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.076, whole genome shotgun sequence.
ACCESSION   DACDUU010000076 DACDUU010000000
VERSION     DACDUU010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.076"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..914)
                     /locus_tag="IWL30_002332"
     CDS             complement(<1..914)
                     /locus_tag="IWL30_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAP9707060.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="IWL30_002333"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="IWL30_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAP9707061.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2391..3650
                     /gene="lysA"
                     /locus_tag="IWL30_002334"
     CDS             2391..3650
                     /gene="lysA"
                     /locus_tag="IWL30_002334"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAP9707062.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3637..4437
                     /gene="dapF"
                     /locus_tag="IWL30_002335"
     CDS             3637..4437
                     /gene="dapF"
                     /locus_tag="IWL30_002335"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAP9707063.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4440..5321
                     /locus_tag="IWL30_002336"
     CDS             4440..5321
                     /locus_tag="IWL30_002336"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAP9707064.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5318..6034
                     /locus_tag="IWL30_002337"
     CDS             5318..6034
                     /locus_tag="IWL30_002337"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAP9707065.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6040..6735
                     /gene="dapD"
                     /locus_tag="IWL30_002338"
     CDS             6040..6735
                     /gene="dapD"
                     /locus_tag="IWL30_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAP9707066.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6751..7752
                     /locus_tag="IWL30_002339"
     CDS             6751..7752
                     /locus_tag="IWL30_002339"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP9707067.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7749..9110
                     /locus_tag="IWL30_002340"
     CDS             7749..9110
                     /locus_tag="IWL30_002340"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAP9707068.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9070..10221
                     /locus_tag="IWL30_002341"
     CDS             9070..10221
                     /locus_tag="IWL30_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAP9707069.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10196..11365
                     /locus_tag="IWL30_002342"
     CDS             10196..11365
                     /locus_tag="IWL30_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9707070.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11540..12919
                     /locus_tag="IWL30_002343"
     CDS             11540..12919
                     /locus_tag="IWL30_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9707071.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            13200..13429
                     /locus_tag="IWL30_002344"
                     /pseudo
     CDS             13200..13429
                     /locus_tag="IWL30_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4484 a         2339 c         2924 g         3682 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaaaaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtataaatag cttctaatag
     2221 ttcgggtatt ttgtttttat aaccattttt attcttattt ttaaaaaaat atttcgaaat
     2281 atactagaca attcagaaaa ttttgtttaa tattattcca ataaaattca aatctttatc
     2341 aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat
     2401 tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca cctctgtatg
     2461 tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc tttgtatcga
     2521 aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc
     2581 atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa atctataccg
     2641 ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac aagacgccag
     2701 aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat aatttgatgg
     2761 aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt atcatgcttc
     2821 ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg catattgatt
     2881 caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag ttgataaaag
     2941 aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa atttttgatg
     3001 ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat tttgaccaac
     3061 ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat gatcagccga
     3121 tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct ttggaaaaac
     3181 gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt ggagaagctg
     3241 gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa tattactttg
     3301 tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa tacgattgtg
     3361 atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt
     3421 gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa tcgggtgatc
     3481 ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac tacaatcgag
     3541 cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt gttcgcagac
     3601 aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa atataatggc
     3661 tgcggaaatg attttatctt attagattac gcagaagaca cagattatag tcaattagcc
     3721 gcttctttat gcacaaaaga aaaatatgat atagacggat tgatcgccgt aaaaatcgat
     3781 ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg cggaaatggc
     3841 attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca ggaattcaac
     3901 gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc tttttattgc
     3961 agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa tcaaacttat
     4021 ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac tagtttattt
     4081 ttaggaacga ttcataccgt tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa
     4141 agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt caactttgta
     4201 gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt aggttggaca
     4261 cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt
     4321 tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc tggtgaggat
     4381 gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga ggaatgaaga
     4441 tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga tcagtagatt
     4501 atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat ggattattag
     4561 ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag cagatccttc
     4621 aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct ggaacgaata
     4681 atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca gacgcacttt
     4741 tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct cattttacag
     4801 caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct agaacaggca
     4861 tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc attggtttga
     4921 aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt ggagaagaat
     4981 tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta ggaggaagcg
     5041 gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta gtaaaagata
     5101 cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt gaactgatcc
     5161 atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca ttattagact
     5221 taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa aagaaacaat
     5281 tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag
     5341 tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata cggtccttct
     5401 tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc cagaactgct
     5461 tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct tatcatatca
     5521 acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag aaataaaaga
     5581 acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca tcatggtgat
     5641 gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg aactgattga
     5701 aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc tgcttcaaac
     5761 aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa aaagagaaga
     5821 aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac atgatgtatt
     5881 gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta atcgaatctt
     5941 tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag gttattacaa
     6001 attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca agaaatcatc
     6061 cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag
     6121 ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt
     6181 gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa
     6241 atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa aattaatgct
     6301 cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc
     6361 atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat gattgatatg
     6421 ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg tgcaggagct
     6481 gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga agacggcgta
     6541 ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg
     6601 gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct
     6661 gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt gattgctgcg
     6721 ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg tattgtgggg
     6781 gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga ttttccagta
     6841 ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa
     6901 aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga tttagcgcta
     6961 ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag tcagggacta
     7021 tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt aatcgttcca
     7081 gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa ttgttcaacc
     7141 attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca gcgagtcaat
     7201 tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt
     7261 acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac ggtgattccg
     7321 gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat gataaatgag
     7381 acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt acgtgtgcca
     7441 atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt tacgacggaa
     7501 gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga tctagcagca
     7561 cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt tgggaggatc
     7621 agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga taacatacgc
     7681 aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa aatagcggag
     7741 gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg
     7801 aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc caagtgatcg
     7861 ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac ttactttatt
     7921 tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa aggatttgtg
     7981 atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa gatgaactag
     8041 cacgcataca gaaacaactg gaagacgaaa agataactga agattattta gtaagtagag
     8101 gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt attgacgcaa
     8161 aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa
     8221 tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac ggggcgaacc
     8281 ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc gcgatactgg
     8341 cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga atcatgatgg
     8401 ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt agagaactga
     8461 gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc ccagttcaag
     8521 aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa
     8581 tatcgaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga cgcaaggatt
     8641 ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt atctggaaag
     8701 caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca gggatcgaca
     8761 atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc atcacgaaag
     8821 aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta gctttgatct
     8881 ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg ttatcgatat
     8941 tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag ttgaatctga
     9001 tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat gaagggatgg
     9061 tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga aatcgggttt
     9121 catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcga ggccgttatc
     9181 catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga agaaacgatt
     9241 gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct ctcttttgct
     9301 tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc gatgctttta
     9361 gggttgactg actttataac taaacacatt gaggatctta tttataatgt agtactgatc
     9421 tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc aggattgttg
     9481 gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact agctaaggga
     9541 gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga cattatcatt
     9601 aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct
     9661 gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga gtggaatcat
     9721 ctaatgaaat ttggccaaat ccaaggtgga acgatccgaa atgttttagc tggagaagtt
     9781 attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt gaagagggga
     9841 ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg ctataatgac
     9901 ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga ttcaaacaaa
     9961 ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata tacgcaacaa
    10021 tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca tgatccgacg
    10081 ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac gattcttttt
    10141 acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt aaataatgat
    10201 gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa aaatacgaca
    10261 attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga
    10321 ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag aagacttttc
    10381 tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta tatttcagca
    10441 acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag ttggtgcaac
    10501 ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg ttcttattcc
    10561 agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg aattgatccc
    10621 gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac gagcatttga
    10681 acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc ctactgggac
    10741 gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt
    10801 tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata tgtccatagc
    10861 tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac atgcgatgac
    10921 tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac taattaaagt
    10981 ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct
    11041 aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac gaagagacta
    11101 tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg gtgcatttta
    11161 cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt gtgtagaatt
    11221 agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag aaggagaagg
    11281 ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct gcaaacggat
    11341 caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa tcgttaattt
    11401 tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg aacgcgtttc
    11461 ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat
    11521 attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg catctcatgg
    11581 agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag atatcacgct
    11641 atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt atttagaaga
    11701 caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc cgcaatccaa
    11761 tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa aaacagtaac
    11821 ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat tgattttgag
    11881 ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa atgtctactt
    11941 gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc cggctattaa
    12001 aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag caagccgaaa
    12061 agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca cttatttaga
    12121 tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg aagttttgac
    12181 aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca aaacaagtga
    12241 aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa atacagaatg
    12301 gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg aatacttgca
    12361 aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt ctatcccagc
    12421 acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac gttatgtagt
    12481 aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag gttcctctgc
    12541 attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta cagcagcaaa
    12601 agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa aatatgtacc
    12661 agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa tgacccatca
    12721 attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa atgtattggc
    12781 actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact tcttcttcca
    12841 gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag cgttaggaga
    12901 agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct
    12961 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta acttggctgg
    13021 acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag ggactgtgac
    13081 acgatttgtg taccgacccc caaaagttag accaaaaaat ctaacttttg gaggtcggta
    13141 ttttttatgg ttaaatatac ttttgaactt aaattaaaga tcgttcatga ttatttggat
    13201 ggcaaaggcg attggaccgt aaaaatccag atcaagagat tgaagcggaa atgcttaaaa
    13261 ttcgggagaa gcacaaggat tatggctgtt tacggatgac caatgaacta agaaaccgcg
    13321 gtttctctat caataaaaag aaagttcagc gcttgatcaa gaagctagga ataaaagtaa
    13381 cggcatacac gcgtaaatca cgtcgctaca attcttatcg aggaaaagt
//
LOCUS       DACDUU010000077        13178 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.077, whole genome shotgun sequence.
ACCESSION   DACDUU010000077 DACDUU010000000
VERSION     DACDUU010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.077"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            145..1884
                     /gene="yfcC"
                     /locus_tag="IWL30_002345"
     CDS             145..1884
                     /gene="yfcC"
                     /locus_tag="IWL30_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP9707072.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2041..3225
                     /gene="iadA"
                     /locus_tag="IWL30_002346"
     CDS             2041..3225
                     /gene="iadA"
                     /locus_tag="IWL30_002346"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP9707073.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3336..3539
                     /locus_tag="IWL30_002347"
     CDS             3336..3539
                     /locus_tag="IWL30_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP9707074.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3640..4302)
                     /locus_tag="IWL30_002348"
     CDS             complement(3640..4302)
                     /locus_tag="IWL30_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9707075.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4315..5817)
                     /locus_tag="IWL30_002349"
     CDS             complement(4315..5817)
                     /locus_tag="IWL30_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9707076.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVTVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6365..8044
                     /locus_tag="IWL30_002350"
     CDS             6365..8044
                     /locus_tag="IWL30_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP9707077.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8171..8650
                     /locus_tag="IWL30_002351"
     CDS             8171..8650
                     /locus_tag="IWL30_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAP9707078.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8869..9549
                     /locus_tag="IWL30_002352"
     CDS             8869..9549
                     /locus_tag="IWL30_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP9707079.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9557..10888
                     /locus_tag="IWL30_002353"
     CDS             9557..10888
                     /locus_tag="IWL30_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9707080.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10903..11865
                     /locus_tag="IWL30_002354"
     CDS             10903..11865
                     /locus_tag="IWL30_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAP9707081.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11976..12314
                     /locus_tag="IWL30_002355"
     CDS             11976..12314
                     /locus_tag="IWL30_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAP9707082.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12393..13100
                     /locus_tag="IWL30_002356"
     CDS             12393..13100
                     /locus_tag="IWL30_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAP9707083.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4197 a         2322 c         2888 g         3771 t
ORIGIN      
        1 tacataggag aaaaaaaaca aaatagaaaa aagtgatttt ctaataaatt attcatgtgg
       61 aaatgtcatt tttactgtat aattcatttg attatccatt ttttattcat caaaattact
      121 gaaaaaagaa aggaagaaat aaatttgaag aaaacaaatt cttcaggaaa accgaaacgc
      181 gacttcacta aattatcgac accccatacg tatgtcatta tttttggtgt agtcatcttt
      241 gcatggatac tgacttttgt cgttccagca ggaaagttca gcacgcaaga tattgaatac
      301 aaagatgcaa atggcgaaac aagtactcgt actgttttac gtcaagattc cttccgttat
      361 gcttacgagc tggataagtc gtatgtgttt gatcaattag aagaactgca agatcatcca
      421 gcagaacgtg aaaaactgga tgttccggaa aaaggattgg aaaaagtcat tgctgacgga
      481 gaaaaaaatc ttacgcagga aaaactagat gaaatctcct tgacagatga tgtgctttac
      541 gatcagtacg gcgaaaatat ctatgatact tctaaaaaat tgcataaaac agccaaaata
      601 tggggaacag atgattttgg cggctttggt ttcttaaatt ttgtttttga aggactagta
      661 tctggagata agtatggatc tgctgtaggg atcgctgcat tgatccttgt tgttggaggt
      721 gcttttggta ttattatgcg taccggagcg atcgacgcag gtatctatgc gtttatcagt
      781 aaaactaagg gactggagcg tttagcttta ccgctattat tctttgcttt ttcattcggt
      841 ggcgctacat tcgggatggc agaagaggta attccattct caatggtgat ggttccattt
      901 gtcattgcgt taggatatga ctcgatcgta gcagtaaccg tgacttatgt cgcatctcaa
      961 gtcggaaatg cgacctcttg gatgagcccg tttagcgtag cggtggctca agggattgct
     1021 ggaatccccg tgttgtctgg tgcgacattc cgattgatta tgtgggtagt tgttactgca
     1081 ttagccgctg gttatatgat gatatatgca gaaaaaatcc gtaaaaaacc agaaagttcg
     1141 ttgacttaca aatcagatga ttatttccgt tctcatatca aaaagacatc tgatgaaaat
     1201 aaaaaattca tgcttgggca taaattgatc ttattagaga tgttagttgt ccttgtctgg
     1261 atcgtttggg gcgtgactca aaaaggctat tacattccag aaatcgcttc tcaattcttt
     1321 gtgatgggat tagttgctgg aatcattgct gttcttttca aattagatgg aatgaaagtc
     1381 aatgacatcg cttcttcttt ccaatctggt gcagctgatt tagctggtac agcaatcgta
     1441 gtaggtatgg ccaaagggat tcttcttgtc ttgggaggat ctgatgcatc tgttccttca
     1501 gctttgaata cgattttgca tgggatcggg acggcgttga ccggtgttcc agctgtcatt
     1561 ggtgcttggg cgatgtacat cttccaaagt ttgttcaact tggttgttac atcaaattca
     1621 ggacaagcag ctttgactat gccaatcatg gctccactgg cagatttagt aggtgtatcc
     1681 agacaagtag cagttcttgc ttatcagctt ggttcaggtt ttatggacgc ttttacaccc
     1741 gtatcagcca gcttgattgg tgtgctaggt gttgcacgga tcgaatgggc aaaatgggca
     1801 agattccaaa tcaagatgca aggattcctg tttgttttag gaactatttt catcatgatt
     1861 gccattgcaa taggtctgca gtagtactgg agacctgtaa ataaaaaagt aatccaaaca
     1921 gtagataagg actccaagaa ccgtcatttt acggtaaaat caaaggagta aagatcctat
     1981 cgtttggatt attttttaag atcctatcat cagttatact taaagaaaga ggaaaaatgt
     2041 atgaaaatca ttcgacaaat tgaagtgttt gcaccagatt acttaggaaa aatggatgta
     2101 ctgattgccg gtgggaagat tttggctgta gaagaacagc tagaaggcgg ctacgaagga
     2161 gtggaagtag aagaactttc aggtgaggga aaagtactaa ccccgggttt cattgactgt
     2221 catttccata ttttaggtgg aggaggagaa ggcggttatc aaaatcgaac gccagaagtc
     2281 acattgagcc aattgactac agctggtgta acgacagttg tcggttgttt gggaacagac
     2341 ggagaaggta gagatatgac tgcgttgatc agcaaagcga aaggtctgga agcagaagga
     2401 atctccactt atgtatacga aggttcgtac cgattgccgg taaaaacagt aacagattct
     2461 atcatcaaag actttttgac aattgataaa attatcggaa tcggagaaat cgcagtatcc
     2521 gatcaccgtt cttctcagcc aagttttgaa gagttcgctc gtgcagtagc agatgctcgt
     2581 gtaggaggca tgttatccgg aaaagctggg atcatcaatg tccacttagg cggaggaaaa
     2641 agaaagctag agctattgac acgaatcgta gaagaaacag aaattccgat cacacaattt
     2701 ttgcctactc atgcaaatcg cacaccagaa ttatttgaag cttgcgtggc atttgccaaa
     2761 cgtggtggca cgattgactt cactgctagc gaagatcctg atttttggga gaagacagat
     2821 ggtgaagttc gcttcagcaa agcactgaaa cgattgatcg aggaagatgt ttctttagat
     2881 aatttcacga tgacctcaga tggacaagga agcttgcctt attttgatga aaacaatcat
     2941 ttcctagggt taggagtagg aagtgccaag gcattgctag tagggatcaa agaagctgtg
     3001 caaaaagaaa gtatcccttt ggaaatcgcc ttacgagcta taaccagtaa tccagcacgt
     3061 atattgaaat tggataaaaa aggaaaaatc gaaattggtg ctgatgcaga tctgtgtatc
     3121 ttggataaag aaacgcttga tattgataca gttatcgcta aaggagaaat aatggtccaa
     3181 gaaaaagaag tcaaagtatg ggggactttc gaaaaaagtt tttaatcaac agctctaact
     3241 atctttttta gaaaacgagc gttctgtttg tctgtatctg ttgttgatgt taccctgaag
     3301 gtagatcaat aaacaaacta agaggaggaa gcacaatggc agatttaaaa ggacgttcta
     3361 aagatatgaa agataaagta gtcggtgaag gaaaagaagc atacggcaaa gcaacagacg
     3421 acaagagcaa agaaacagaa gggaaagcac aatcactagg tgctgacatc aaagaaaaag
     3481 ctcgaaattt aggcgacgaa gtcaaagaca agttttctcg aaaagacgaa aaaaaataaa
     3541 aaccacttat tactaattga atcaagcaaa taaaaaatcc gaaccatatc tgcgagacag
     3601 atatggttcg gattttttat ttgatcatta tcctgttttt taaacttcga ttttttgaaa
     3661 ttttttattt tttagttcaa agacttcatc acagcttttt gctacttcaa tctcatgagt
     3721 cacgatgata atacagcgat tttgttcatg ggcgatttct tggaaaagtt tgacgatatc
     3781 ttgtgaagtt gtttcatcta gatttccagt aggctcatcc gctacgatta gttgatgttc
     3841 acagcagata gcacgaacga tagcgactcg ctgctgctga cctcccgaaa gtttggtcac
     3901 ctgttttttt gctaatattt catcaattcc tacacgagcc agtcgatcta aagcaacttg
     3961 tcgataattc tcttgcttgg tcttagtgat ttccattgct gctatgacgt tgtctagagc
     4021 cgataaataa ggaagcaagt tgtaagcttg gaaaataatc gagacatcat ttttacgata
     4081 acttcttaac ccgattttgt tcagtgaacg atcttgatag tatacctttc cttcttttgg
     4141 tgcatctagt ccagaaataa gcgaaagaaa agtcgttttt ccagaaccac ttgctcctaa
     4201 aatagcgtac atttttccag cttcgaaatt cagttctaca tctttataaa gcgcatcttg
     4261 cggatcttga taccaaaatc ccaattgttc tgtccgtaac atcaaattcc tcctttagtt
     4321 taataggatt tttcttgggt ttaatcttaa tatccctata gaagctaaaa agacagaaaa
     4381 cagactgatt ccaaatgcta ctccggctaa tagtcccaat tgtgcaggtt tgacattgat
     4441 ttccaattcg ctgacttgat cagaaacagc aaatggatta ttcgtggttc cttgttgacc
     4501 atcgcgttga ccgccttgtt cttgtctgtt acctggcatt tgtccaccgc caggagcttg
     4561 ctggttttgt gtcgtggttt ctgtctgttg gtcaagtagc tgctgtccca cggcattcgc
     4621 gacgacgttt ccgctaagcg aagcaactcc taatgcaaga atcatacaaa tagccacttc
     4681 ggtaaacaat tggagaatga ttttacttct ggattctcct aaagatagca agacgcctaa
     4741 ctcatgccga cgctcccgaa tggtaatcat aatgatcaga gtaagtatga taataccggc
     4801 tacagtcact agtaaaacaa catttttaga aaaacttgcc acgttgttta aaggttccaa
     4861 cattgactga tacatggaat catttgattg taagctaaat gtatctgtgt cgatcagctt
     4921 ctcagcttct ttgagaaact catccatttt atctggatca gatagagtgt aaatagcaga
     4981 atctatcgtg ttatcgtcag atgtcccatt tagttcattt gcaaaagtgt aagaagtgta
     5041 aagtgtattc gacggattca tgaagttgaa tttcataccc atactgccgc ttgtttcgct
     5101 ggattcataa attcctttga tcgtcatttc ataagtggta tcttcatctt cagtactcgt
     5161 gattgtgaaa gtatctccga ctgccaggtc atttgtttct gctagagtag aatcaatgac
     5221 tacattgttt gtgcctttgt cgctgtcatc tattccttca ccatccgtga tttttgctgt
     5281 tccatcagaa aaattactat tttgtgcagt tgctgacaca ccactgattt gaaagtctgc
     5341 ttgagtcatt tgcggattcc cgcccattcc tccagcaggt tcaccgcctg catcagtagt
     5401 ttctgaactg tcttcacttg aatcagaact agtgatggca gttattccat cttttgccat
     5461 agctgaggcg gaactttcaa aggaataact ctttacatta tctaattcag ctattttttc
     5521 cgcgtccgtt aaggaaactg gtgtaagaga aaaactgcct ggatctggac gttcttcagt
     5581 ggtagaaccg cttgaagaag tttgttcctg ttttttaaat gaagcttctc gattggcact
     5641 taaagtcact gttgctccaa cttctttttg tgcttgattc gctgcttctt ctgcagcgct
     5701 tcgaatcgtg aggccagcta gcacaaaaat aagaatggct gtgaacacgg caaacaataa
     5761 caatgaacgt ccccatttta cttttgtact tttccatgca cgtttcataa aattcatatt
     5821 ttgcatccct cctagtagat gcatttgata atagacaacc tatcttaaag ctagcttaaa
     5881 tcttatgctg tttgcacacc atcaacatga ggttctgtat atataaaaag tcactatagt
     5941 tattgcaaaa aatgtggtaa aatgaaaaaa gtatgtttca aaccgacgaa gaagaaatga
     6001 aagaggaata gcaaaagaaa tcctagatgc tacagaggga tttttacgtg catagttgtt
     6061 ctaacaattg ctgatactag cgatttagtt tatttgttgt ataataatgg ggaacagctg
     6121 aatacttata taagtattct ggctatagcg tttcgcaaaa gatccatttt gttaatgaag
     6181 tatatccaaa ataaaatgcc catccaactg tcagaacgcg gatgtgctgg taattttcct
     6241 gctgattctc cataagttga gaaatagtga ttttgcatat gcttcattgg aatggctcat
     6301 atatttagat aaagaagaaa atcgacatct ttaaacttgg atgtcataag aagaggtgaa
     6361 gtttgtgagt accattaaaa ttatcccatt aggcggcgtt cgtgaaaatg gaaaaaacat
     6421 gtatgtcgca gaagtgggcg aggatatttt tattttagat tgtgggctaa aatatcctga
     6481 aaatgagttg ttggggatcg acgtcgtaat ccctgatttt acgtatttag aagaaaacat
     6541 tgatcgtgta gctggtgtct ttttaacaca tggacatgca gatgcgatcg gggcacttcc
     6601 ttacttgttg tcaaaaattt ctgtaccagt attcgggaca gagttgacga tcgagttagc
     6661 aaaattgaat gtcagtcgta atgaagcagc caaaggattt aaggatttcc atgtggtaga
     6721 tgagcataca gaaatagact ttggtgaaac agtagtgagc tttttccgta ccactcatac
     6781 gataccagat tcagtgggga tcaatctgaa aacaaaagaa ggtagtatcg tttatacggg
     6841 agattttaaa tttgaccaaa cggctatccc aatgtatcaa acagattatg cacgattggc
     6901 ggaaatcggc aaagaaggag tcttagcctt actaagtgaa tcggcaaatg cagaaaatcc
     6961 tcagcctgta gcttctgaag cccaaatagc agatgaagta tttgatacga tcaaatattg
     7021 ggaagggcgt atcatcgtcg cttgtgtagc aagtaatttg caacgagtgc aacaagtatt
     7081 ggatgcagca gaaaaagcac aacgaaaagt tgtcttgact ggtcaagatt tcgaacgaat
     7141 tattcgcaca gcgatgcgtt tagaaaaact tcaattacca agtgaagatc tgcttgttac
     7201 gttgaaagac atgaaaaact atgatccaga agaactgatc attttagaaa ctggccgaat
     7261 gggtgaaccc atcaagtctc tgcaaaaaat ggcaaacggt acacaccgta atgtcaagat
     7321 tgaagaagga gatttggttt atatcactac tacgccaagc atcgcgatgg aaaccatcgt
     7381 ggcgaaaacg gaagatatca tttatcgagc tggtggaaca gtgaagttga tctctgagaa
     7441 tatgcgcgtt tctggccatg ccaatcctaa tgacttgcaa ttaatgatca atttgatgaa
     7501 accaacatat tttgtgccag ttcaaggaga gtatcgtgaa ttagctgctc atgcagattt
     7561 ggctcatgca gtagggatgc catacaaaaa tatctatatt actggacgcg gagacgtgct
     7621 ggaatataag aacaagcgta tgagtgttgc aggaactaca agtgcagaaa atgtaatgat
     7681 tgatggatta ggcataggtg atatcggaaa cattgtttta cgtgatcgaa aaatcttgtc
     7741 agaagacggg attttcgtag cggttgtcac tatcagtcgg cgagaaaaga aaatcatttc
     7801 aaaacctcaa attacttcac ggggattcgt ttacgtaaaa gctagccgtg atctgatgaa
     7861 agaaagcagc aatatgattg aaacaatcgt tgagaaacat ttagaaagca atgattttga
     7921 gtggagcaaa ttaaagcaag acatccgaga tcaattaggc cgttacttat ttgaacaaac
     7981 aaaacgccgc ccagtgatct tgccagtcat tatggaagct actcaacgtc gaaataaaaa
     8041 ataaacaaaa agagtaagat gaacctctct atagagtgct cgtcttgctc tttttttgcg
     8101 aaaaacaaat aatttttgaa tacagcatcc ctcgctttat aataaacgat agaataaagg
     8161 gagtgaatca atgatgagtc aaacaaaagt cgaacgttat ttagaagaac aaaagatcag
     8221 ctatcaacca cttgatttta gtcatgtggt agaagaatct ttcacagaag aactacaaaa
     8281 aagagggatt gatccagctt tgatatgtaa aactctcgta gtgaaaggaa acaaaactgg
     8341 ggtgatcatc gcattgattc ctttgaacga tcacttagat tacaaaaaaa cgagacaagt
     8401 gacgaaagat cgtaaagttg gttttcctgg aatggatttt gtattaatgc atacaggata
     8461 tccccacgga gcaaacaccc ctattggaat aaaactcgca catcctgatt attgtttttt
     8521 agcagacagt tctttaaaag aaaaagaaga agtaattgtg tcaagcggtg aaatcgggag
     8581 aagccttcgg ataaaagtag ccgatttaga aaagatcgtc catcctattt acgctgattt
     8641 aattagttaa gggacgagaa gagaagctaa gcagagcaga gacctcaaac ttgagattat
     8701 atctgcttgg tacttggtgc atcctgtatt atctgttaca attgctttgt acatactgta
     8761 ctatcaatcc aatgaaaata tatttagcag ctatattttt tcattgatgt gcaagtgatg
     8821 aaatcgtttt taataaaaaa gatacagaga aaacgcgagg tgtaaaatat gaaaattacg
     8881 tatcatggac attcctgttt aacaattgaa atggaaaatg gccaacgcgt gatgattgac
     8941 ccgtttatta caggaaatcc attgacagac ttaactgcag aagaagtaaa gacagattgg
     9001 ttgctgatta ctcatggtca tagtgaccac ataggagata tgattcctat tgcaaaagaa
     9061 aatcaagcca caattatcag tattgttgag attgctgatt atgcgaacag cagaggagta
     9121 gattcattcg gtatgaatat cggtggaaaa catgcgtttc cattcggcgt ggtaaaattc
     9181 gtacatgcac agcatagttc aagctatgaa gtagacggta tcgttcagta tatgggcgag
     9241 cctagcggaa ttattattca agcagaagga aaaacgattt atcatgcagg ggatactgct
     9301 tatttcagtg atctaggatt gcttgcagaa gaattcgata ttgatgtcgc tttcttgccg
     9361 attggcgata actatacgat ggggccagaa gatgcagcaa aggctgcgca aacaattcgt
     9421 gccaagaaag tagttccaat ccattacaat actttccctg tcattgaaca agatcccgaa
     9481 tcttttgtag agttactgga agatagtgaa ggaaaaataa tgagcgtctc ggaaacgctg
     9541 gaagtgtagg cagaaaatgg cgacaaaaca tgatttgatt ttagaatata ttgaaagttt
     9601 acctgttggc aatcgaattt ctgtaagaag catcgcaaaa gaattgaatg tcagtgaagg
     9661 aacagcgtac cgtgcaatca aagatgcaga aaatgtcgga ctagtttcaa caattcaacg
     9721 agtagggacc atccgtatcg aacgcaaatt aaaaaaacat attgaacgtt tgacatttgg
     9781 cgaggtcgtc aggatcattg aaggggacgt tttaggcggg gcggcaggat tagacaaggt
     9841 cttgaataaa ttcgtcatcg gcgcgatgac taaacatgcc atggaacgct atattacgcc
     9901 aggatcacta atgatcgtcg gcaaccgggc ggaagtccaa aaattagcat tggaagatgg
     9961 cgccgcagta ttgatcacag gaggattcaa tacgactgct gagattgcaa agctagctga
    10021 tgatcttgaa atgccgatct tacgcacctc ttatgataca tttacagtcg caaccatgat
    10081 caaccgagcg ttaagcgacc aattgatcaa aaaagatatc atgcttgtca gtgatatcta
    10141 tatgccagct gaaaagactc attatttgca gcagacagat acgatttctg attatcagcg
    10201 tttgtcagaa gaaacgcagc attcgcgttt tccagtcgtc aaccgccatc atcgtttgat
    10261 ggggattgtt acggcaaaag atgttttagg gaaatcgcca aaccagatca tcgatcgggt
    10321 gatgacgaaa gaaccaatca gtgtaaagaa aacaatgagt attgcctctg ttagccatca
    10381 aatgatctgg gatgggctag aagtcatgcc ggtcgtttcc gatgacttga cattggaagg
    10441 cttgattacc cggcaagatg taatgaaagc gatgcagctt gtccagcgac aaccgcagat
    10501 tgcagatacg atttcggatc agatatctgg aaatatccat acgattgata ctgaccgtga
    10561 aggaaatcgt ttggaaaacc ctaagttcaa attcgtagta gcaccacaaa tggtcaatgg
    10621 cgttggaacc atttcttttg gcgtacttag cgaaatcatt tctgatgtcg cacaaaaaac
    10681 aatggtgatg aaccaaaaac gaaatatctt gattgaacaa gtgaacctac attatttgcg
    10741 gctgatccaa ttggaaagcg aattagacat tcgtccacgt gtattggaaa tcggcagacg
    10801 ttccgctaaa ctggatatcg aagtgtttat tgaaaacacc atcgtttcca aagcaatcgt
    10861 tgtttgtcaa gtaatggaac gttcttagaa agcaggagtg aaatgacagt acagcaagaa
    10921 atagtagata caataaaaaa atacgataaa atcattatcc acaggcattt gcgccctgat
    10981 cctgatgcat taggatcaca agtaggatta gccgaactat tacgggaaag tttcccagaa
    11041 aaagaaatac gtcaagtagg tgaaacgatt gaagggcttc gctttttagc agaaatgcaa
    11101 gaagttgaag atgtcttcta ccaaggagcg ctagtcatcg tgactgatac agctaattca
    11161 cccagaatca gtgatcaaag atatcagttg gcagaaaaac tgatcaaaat cgaccatcat
    11221 cccaatgatg acccttatgg agatcttgtg tgggtaaata caaaagcaag tagctgtagt
    11281 gagatgattg cagaatttgc tttgatgttt cctgaatctt taaagatgaa cgttaaggct
    11341 gcacggttac tttatgcagg aatcgtcgga gatacagggc gctttttgta tccctccacg
    11401 acgtcccgta cattagagat tgcttcgata ttgagaaatt atccatttga tgcaagtgct
    11461 ttgaacagag agattgaaca aatgccgatg aaagtcgcta aactttccgg ttatctttat
    11521 caaaatatcc aggtggatga aaatggtgca ggaagaataa tccttccgca agaaattctt
    11581 gataaatatg gaatcgatga ttcagagaca gcttcgatcg tttcacttcc tggagtgatt
    11641 gacgaagtgc tggcttgggg gatttttgtt cagcagcctt cagggtatta ccgcgtacgt
    11701 ttacgttcta aggggccgat catcaatgaa ttggctaaac gccatcatgg aggaggccat
    11761 ccattagcaa gtggtgcgaa tgcaaaagat ttagcagaag tagaggaaat ctataaagag
    11821 atccaagata tatgccaatc gtataagagc agaaaaacag aatgatactt cgttcgaaaa
    11881 cgtgcctttt gtagtaaaaa tcacaaatgt atcttttcaa gatcttcttt ttccgttaca
    11941 atagtgctca ctaaagattt taaaggagaa agaagatgac attaccaaat tgcccagaat
    12001 gtggctcaga gtacacatat gaagatcgag gcttgttgat ttgcccagaa tgcgggaacg
    12061 aatggaacaa agaagaacaa gaaactgtca tagaagacgg attagtggta ttagatgcaa
    12121 atggaaaccg tctgttagag ggcgatagcg tgacagtgat taaagacttg aaagtaaaag
    12181 gtgcgtcagg tgccattaaa caaggaacaa aagtcaagaa catccgttta gtagaaggcg
    12241 atcataatat cgattgcaag gtagaaggat ttggaccgat gaaactgaaa tcggaatttg
    12301 tgaaaaagaa ttaaagaata ttcaaggtct tttcacattc ttttcctact actaatgata
    12361 aaataaagta aacgaaaagg agtgaaacga aaatgtatgg ttatggaatg tatggttttt
    12421 tagatccgac atttcttttg gtcattcttg gattagttat ttccggaatt gcgtcagcgt
    12481 acgtcaatag tactttccga aaatacgatc aagtgagaag caaaaacaat gtgactggta
    12541 cacaagcagc acaatatata ttacaaagtc aacagataaa taatgtcggt gttcaacaaa
    12601 ttgccggaga tctcacagat aactacaatt caggtaataa gatgttgagt ttgtctcaag
    12661 caactgcaca gtctacttca gttgctgcga taggtgtagc agctcatgaa tgcggacatg
    12721 cggtacaaga tgcttcgggt tatgtcccat tgaagattcg tgcagcactt gttccagcag
    12781 ctaactttgg ttcgatgatt tcgttcccct tgattatggt aggtgtattg cttagctgga
    12841 actctacgtt aatcaatatc gggattttcg ctttttcttt agcattactt ttccaattgg
    12901 tgacattacc agtcgaattt aacgcgtctc ggcgagcaat ccaaattttg agtgaaggcg
    12961 gactactgac ggaagaagaa gtaccgatgg caagacacgt attatttgca gcagcactga
    13021 cttatgttgc agcagcgctt tcgacattcc tgcagctgtt gcgcttgatc ttattgttcg
    13081 gcggcaaccg aagagattaa aataaagaag ttgtgaaatc agcgctttga tctgagctaa
    13141 taccgagagt tagctgattg aataccattt atttggaa
//
LOCUS       DACDUU010000078        12734 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.078, whole genome shotgun sequence.
ACCESSION   DACDUU010000078 DACDUU010000000
VERSION     DACDUU010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.078"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IWL30_002357"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IWL30_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP9707084.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="IWL30_002358"
     CDS             complement(322..891)
                     /locus_tag="IWL30_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAP9707085.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="IWL30_002359"
     CDS             1077..1979
                     /locus_tag="IWL30_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9707086.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IWL30_002360"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IWL30_002360"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAP9707087.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IWL30_002361"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IWL30_002361"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAP9707088.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IWL30_002362"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IWL30_002362"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAP9707089.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="IWL30_002363"
     CDS             8547..8732
                     /locus_tag="IWL30_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAP9707090.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="IWL30_002364"
     CDS             complement(8794..9990)
                     /locus_tag="IWL30_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9707091.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTLLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="IWL30_002365"
     CDS             complement(10160..11596)
                     /locus_tag="IWL30_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP9707092.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="IWL30_002366"
     CDS             11889..12698
                     /locus_tag="IWL30_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9707093.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2673 c         2249 g         4009 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcagtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gaca
//
LOCUS       DACDUU010000079        12526 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.079, whole genome shotgun sequence.
ACCESSION   DACDUU010000079 DACDUU010000000
VERSION     DACDUU010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.079"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            139..459
                     /locus_tag="IWL30_002367"
     CDS             139..459
                     /locus_tag="IWL30_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707094.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            911..1111
                     /locus_tag="IWL30_002368"
     CDS             911..1111
                     /locus_tag="IWL30_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9707095.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1258..4047
                     /locus_tag="IWL30_002369"
     CDS             1258..4047
                     /locus_tag="IWL30_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP9707096.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4094..4621
                     /locus_tag="IWL30_002370"
     CDS             4094..4621
                     /locus_tag="IWL30_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP9707097.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4642..5832
                     /locus_tag="IWL30_002371"
     CDS             4642..5832
                     /locus_tag="IWL30_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9707098.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5872..7170
                     /gene="asnS"
                     /locus_tag="IWL30_002372"
     CDS             5872..7170
                     /gene="asnS"
                     /locus_tag="IWL30_002372"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAP9707099.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVPEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            8336..9010
                     /locus_tag="IWL30_002373"
     CDS             8336..9010
                     /locus_tag="IWL30_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP9707100.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECVNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQKAGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            9007..9348
                     /locus_tag="IWL30_002374"
     CDS             9007..9348
                     /locus_tag="IWL30_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707101.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLI
                     AVTKEKQIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTKEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            9378..9737
                     /locus_tag="IWL30_002375"
     CDS             9378..9737
                     /locus_tag="IWL30_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAP9707102.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            9940..10119
                     /locus_tag="IWL30_002376"
                     /pseudo
     CDS             9940..10119
                     /locus_tag="IWL30_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            10261..12342
                     /gene="cadA"
                     /locus_tag="IWL30_002377"
     CDS             10261..12342
                     /gene="cadA"
                     /locus_tag="IWL30_002377"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP9707103.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
BASE COUNT         4189 a         2177 c         2692 g         3468 t
ORIGIN      
        1 aaaagaggtt gtgacgtggt cacgacctct ttttgacgat tatgatacaa aaaacatcat
       61 ttatgaacaa aatgtgaata acgggcgttc ttgagatatg attttaattg ggaaaaactt
      121 atcgataagg aggtctttat gaaaaaaaga ctgttatggt gtttgctgtt tctatcccta
      181 agtttaacaa gtcagccagt ttttgctgaa gaaacacagc ggtctcctat tacaggcaca
      241 cgtctgtaca aaaatgttta tgtacagaca acagatcttg attctgcgat tcctgaaaca
      301 gaagaacggt ctttgcagcc aagatttggt tacttttggt actgtccgac ctgtgattat
      361 gaaagttgct ggcatttctt acattctaca gctgcagctt ttgcttttac tcatactcag
      421 aaatatgatg gacatcatcc tgttatttat gaggtataga tatgagcaaa aaagaaaaac
      481 taatctcgtt gatcgtattg gtttgccttg ttcttctggt aactgtcata ggctatcaga
      541 aataccatga gaacatcaga cctactaaag aatcttcccc agcctcgacc gttgaaacag
      601 aaccgataag tagtcagaaa gaataatttt tttagtttaa agaagaacat cagagcaaaa
      661 aaacaccaac tgaacaatat gtcgtttatt ttaaaaaaag ttcggttttt cactttatgt
      721 aatcgctgag aaaacttttt ttaataaaaa aagtcgaaaa actctagctt ttttgtaaac
      781 ggtatgatat actataggag tagtttttgt tcggtaatct gtggtagctt gtttcaaata
      841 aacatcttcc agatatacac cattcaagta attgcaaaat atgtgttgat acacaaggag
      901 gatttttatt atggaaacag gtacagtaaa atggtttaac tcagaaaaag gatttggatt
      961 catcactgca gaaaacggaa acgatgtatt cgttcatttc tcagctatcc aaggcgacgg
     1021 attcaaaact ttagaagaag gccaagcagt aactttcgat gttgaagaag gccaacgcgg
     1081 acctcaagca acaaacgtta ctaaagctta attaacctat actaaaaaat aaacgcccag
     1141 acatttgcgt gtctgggcgt ttttgtctat tttctttaaa aagcgatgag agtatttttc
     1201 tgcactttgt agtataattt caaaagaaca ttagaggcag gagaagagga agatattttg
     1261 aaaccaagaa cttatgcagt cgttgatatc gaaacgactg ggacgaatcc aaaagaagat
     1321 cggatcatcc aatttggctg cgtcatgatt gaatcaggtc ggatcacttc acgtttttcg
     1381 atcgatattc atccagggcg gaagatttca aaacaaatcc agcatttaac agggatcacg
     1441 aatcaacgcg tccaaaaagc gccttatttc gaagatgtcg cccaaacgat ttacaatctt
     1501 ttggcagaca ccatatttgt cgcgcataat atttattttg attataattt tttgaaccat
     1561 gaactcatgc gatgcggact tcctagtttg aagataccag gcatcgatac agtagaactt
     1621 gcacaaattt tcttgccgac ggaaccaagc tttcgcttag ctgatttgtc agaaagcctt
     1681 ggatttattc atgagaatcc tcatcaggca gatagtgatg ccgaagtgac gggtcaactt
     1741 cttttgctga tcgaagaacg gatgagaaaa ttgccaatca tcacgttaga gcagattgcg
     1801 cgattaagta gacacacagg tatggatacg agtcgtttca tttatcatgt gattgaagaa
     1861 atgaaagaaa aaccagagcc acttgatccc tcattagaaa tcgtcgatgg gctagctttg
     1921 cagaaaaaag aagtggaact attcacatcc gtccattatg gcgagcggac atatccaaga
     1981 aaaaaacaag caaaagaaaa aatgtttggt aaaacactga tgtatcgaaa agaacagaac
     2041 agactgatga atgcggtcta cgaccatttc acaaaagatg aatcaaaaga tttgatgatc
     2101 gaagcagcta caggaatggg taaaacaatc ggttatctgc tgccattgag ttatttggct
     2161 acacctgaaa aacccgctgt catcagtacc gtttcgctag ttttgcagca gcagatcata
     2221 gaaaaagaca ttccattgct gaatcaattg ctagatcagc ccatacaacc tgtaattatc
     2281 aaaagctatc gccactatat tgatttacag cgttttaaag gcacacttgt ggaaccgcca
     2341 gaacaaaaac aatatgcttt gtatcaaatg gcggtcttag tttggctgac gcaaacgaag
     2401 accggtgatt tggatgaatt acatttaaca aatttgaacc attcattctt tgcagatatc
     2461 gcgcacagag gtattggatt tttagcaaga aatcagtcat tttacgaaca agactttgtt
     2521 cgtttcttgc aaaagaaaat caggcaaagt aattttttga ttgtcaatca tgcgttttta
     2581 atacaagaaa cacagcgaaa agaaccattg cttccagcta gtccattttt gttgatcgat
     2641 gaagtacatc atttgccgga aatcgctgaa aaagtaaatg acaaacgatt aagtattaac
     2701 tatttccata aacaaatcca gcaattagaa gaaccaaatc agctgtttga ccgaatacaa
     2761 agctatttac cgaaagaaca tgaagcctta cgctggttat cgatttatcg agaagaactt
     2821 tctgcgctat gcgaagcaca gatttgtatg gcagaaggat taatgagttt agtgagcggc
     2881 aagcaaccta acggacagta tccagaagaa gtcatgatga caaaagagat gttggatcag
     2941 ctgccactgg aatctgggac agccattgat cacctgcagc ttttttatca ggagatccag
     3001 cagctacagt cacaaatgcg tctgactttt gagattatcc aagaaagctg gacaaaccag
     3061 atgcggatcg atttagcaag tttatatagc ctatttgacc aattagacaa gcagtacttg
     3121 ctgatggatc aatggctgga agactggcga gatcatttga tccattggtt cgttcctggg
     3181 aacacagcgc agcagatgat ttttcatctc catgattttc aagcggctat gttgccgtcg
     3241 actgtttggt atcctcgata tgatcgtatt ctttatattg gaggaacgct gaagatcggc
     3301 cctaatcgac actatctctc taagaggctt ggcattgaag cagcacctct taaagtgatt
     3361 gcctctcctt atgattatga gaaacaggca aggatctatg tgccaaaaga ggcacctgcc
     3421 gtccatcaag tcagcaatca agcatatatc gattatttag agacagcact tacaggtctg
     3481 atcgaacaag aagatcggcc aatacttgtg ttattcactt ctcatgatgt tttgcagaag
     3541 gtctatcaga aaatgcatct gtctatgcta gaaaaaggac gagaaatact agctcaaggg
     3601 tttggcggaa gccgggagaa gcttttgaaa cgcttcattt tgtcagaaaa aagcgtattg
     3661 tttggcgctg acagcttttg ggaaggtgta gatttgcctg gagatgcttt gcagatcgtt
     3721 gtagtcacac gtttaccctt cgataatccg cttcgcccga tggtcaaagc taggaacgct
     3781 tatttagaat cagaggggat caatcctttt taccaggaat ccgtacctaa agcagctttg
     3841 aagttacgcc aagcattagg tcgcttgata aggacggaga cagatcgtgg cgtattgctt
     3901 attttggatc gaagactagt cactgcaaaa tatggtgcac ggatcatcca tgcattaccg
     3961 aaaaaagtac cgatcaaaga gctagctatg gaggaaatct atcaggatat ccatgaattc
     4021 ttaaagaaaa atgaagaagg aagttagagg agctgtcttt tttcataatt aaaatctgct
     4081 ataatgaaaa ggaatgaaga taaaggagcg acgacccttg aagaaaaaac agacaaagga
     4141 aaaaacgatt aatcgcgtgt tacttggctt gatcgtattt ttattagcgg tcatcgtgat
     4201 cagctcgatt ttttacttgc gttccagtcg tccggcggtg caggcgaaga aagaagcagt
     4261 agaaatcgca aagaaatatg caaatcttga ggaagccgat catttttatt ggtttactcg
     4321 aaagaagaca tacttttcac tgacaggtac aaatgataaa ggaaaagaaa tcgccgttat
     4381 cattcctaaa tctggtgaaa aagtaaaagt attggatcaa acaaagggac tgactgaaaa
     4441 tgaagcaaaa cagacaattg ctgcggccca tcctgaaatc caaatcgaaa aagctgcttt
     4501 agggatgtat gatgatcagc ctgtgtggga agtagtagga aaagaaacaa atgggaaact
     4561 gaattattac ctgattggct ttgaatctgg agatgaagtc aagacaatca agggaattta
     4621 aaaggtaaag gaatgaatgg gatggagtta tcaaaaagaa caaaacgatt agaaccgtct
     4681 gtcactttgg cagctgcggc aaaggccaag gcattaaaag ccaaaggcag agatgtgctg
     4741 agtttgactg tgggagaacc ggattttgcg acaccagaaa acatccaaga agcagcaatc
     4801 gaagcaatcc gcaatgggaa agctagctat tatacaccaa cagcaggaat cccagagctt
     4861 cgacaggcaa tcgttgatta tctgaagaaa aactatggac ttgtctatga accttcacaa
     4921 acagtcgtaa cagacggagc gaaatttgct ttgtataccc ttttccaaac gatccttgac
     4981 ccgcaagatg aagtcatcat tcctgtgcct tactgggtca gttacggtga gcaagtaaaa
     5041 ctggcagaag gacttcctgt ctttgtgaaa ggggaagaat ccaatagctg gaaagttact
     5101 gttgagcagc tagaacaagc acgttcagaa aaaacaaaag ccgtaatcat caattctcca
     5161 tcgaatccga ctggaatgat ttatagcaag aatgaactgc aggcaatcgg tgaatgggca
     5221 gtaaaaaatg atattttgat agttgctgat gatatttatg gccgtttagt ttataatggc
     5281 aatgagttta ctccaattgc aacgatttca gaagcaatca aaaatcagac catcatcatc
     5341 aacggcgtgt ctaaaacata tgcgatgaca ggctggcgca ttggttatgc ggtgggaaat
     5401 cctgtgatca tcaatggaat gatcgctgtt gcttcccaat caactagcaa tccgacagca
     5461 gtcagtcaat atgcagctgt tgaagcattg aaaggcgagc aagatacagt ggaagaaatg
     5521 cggaacgcct ttgaagaacg cctgaatacg ttatatccgc ttgtggcaga actgccgggt
     5581 gtttctcttg aaaagccaca aggagctttt tatctttttc ctaatgtaaa agaaacgcta
     5641 cgcatgtgta aatatgaaaa tgtcacacaa tgggtagaag atcttttaga agaaacaggg
     5701 gtagcgcttg tgactggcga aggctttggg gcaccagaaa atgtccgcat gagttatgcc
     5761 actgatcgcg ctacgttaga agaagcggta cgcagaatca agcaatttat tgagagtaaa
     5821 agtcaaaatt aatcctgaag aaattcaagg aatataaaag gagagtcata cgtggaacaa
     5881 attcaaatca tcgattcgaa aaaccatgtg ggagaaacag taacaatcgg cgcttgggtc
     5941 gcaaataagc gatcaagcgg aaaaatcgca tttttacaat taagagacgg aacagccttt
     6001 ttccaaggtg tagttgtaaa aagcgaagtg ccggaagaag ttttccaaac agctaaaaat
     6061 ctgcaccaag aaacatccgt gttagtaact ggagaaatcc gtgaagacag ccgttcaaaa
     6121 tttggttatg aaatcggaat tacaaatgtg gaagtggtaa gcgagagcca cgattatccg
     6181 atcacaccaa aagaacacgg aacagacttc ttgatggatc atcgtcattt atggctacgt
     6241 tcttcaagac aacatgcgat tatgcagatc agaaatgaaa tcattcgtgc aacgtatgaa
     6301 ttctttaatc aacatcattt tgtcaaaatc gatccgccga tcttgactgg ttccgcacca
     6361 gaaggcacta cggatctatt cgaaacaaat tactttgatc aaaaagctta tttatctcaa
     6421 agtggccaat tatacatgga agctgctgct atggctttcg gaaaagtctt ttcattcggt
     6481 ccgactttcc gtgctgaaaa atcaaaaaca cgacgtcatt tgattgaatt ctggatgatc
     6541 gaaccagaaa tggctttcac tcatcaagaa gaaagtttag aagtacagga acaatatgta
     6601 gcattcttag tacaaaatgt tttagatcat tgcgaatacg cattagatgt actaggacga
     6661 gacaaagaag tcttaaaaac ttatacaaaa ttgccattcc ctcgtattac ttatgatgat
     6721 gcgatcgaat tactgaagaa aaatggattt gatgacatcg aatggggcga agactttggt
     6781 tcacctcatg aaacgttcat tgcgaattca ttcgaacagc ctgtctttat cctaaactat
     6841 ccaaaagcta tcaagccatt ctacatgaaa ccacatccaa caagagaaga cgtggtaatt
     6901 tgtgcagata tgatcgcacc agaaggttac ggtgaaatta ttggcggaag cgaacgggca
     6961 acggactatg atttcttgct agaacagatc cgtaatcacg gtttggatga aaaagaatat
     7021 tcatggtatc ttgatctacg taaatacggt tctgttccgc actctggttt tggtctaggc
     7081 cttgaacgaa cagtcacttg gattgctggt atcgaccatg tgcgtgaagc tattccattc
     7141 ccacgtttgt tgaaccgaat ttatccataa gaggtgatga aaaagctgtt ctgcatcaag
     7201 taattagaac agccaagcaa aaacagctgt tcatgttttt gaattggcgg gacttattac
     7261 cgcagatgta agcttttgaa taccgtttag tggagaggtt gtgaaaaagc tgtttagttt
     7321 cgagtattaa gaaaaatttt gtggaaatgg ctttccacat ttttgcataa atttggctta
     7381 ataccgagaa actggctttt gaacaccgtt taaggaagag gttgtgaaat cagtgccttg
     7441 acctgagtat tagagaaaca aaacggaaaa atagcttctc atattttacc gtttttttag
     7501 ctaatatcga gggtcagctg attgaacacc gtttatatca agaggttgtg cgcctgccgt
     7561 tcagctccga gtcacaagga gtaatttcaa aaaacagttt ttcatgtttg atgactattg
     7621 tgacttgtgg ccggagagca acgtttattt aaaaaaaaga actgcgaact ctcgcagttc
     7681 tttttttaaa atacgggata atggaagttt ccagcagaca agagaagaaa gcaaaagaaa
     7741 ttgctatttg attcacaagg ttctataacg aaatcgcaat caatcaaaca tttacaaaga
     7801 aggtaattca aatggaaatc tgatttgatg atgcttctgc tgttgaaata aagcagcatc
     7861 tagcacctga aaacaaacta atagtaacat tcgaagatgg agtaggtccg tattttcagc
     7921 atgcaataat ccatatgtaa gttcaattca cccttgaaat catcggacca gataaagtta
     7981 tgaccaatct atcccaagca atctagaaga gatattagta aagggacatt tactagaagt
     8041 cttagatgag cctataaata tatgttttga tggcgtcatc agtggtctga ttgacggaag
     8101 cttgggtatg atcgatctct ctggaccaaa caacgaagga atcaagggga atccggcacg
     8161 ttaagaaaaa aatagcataa aaaactagac acttgatgag cgttcattca attgtttgaa
     8221 gggactaggc tggtgtcatt ttttatacat atattttctc tttcatcttg atagggctta
     8281 tgggataata taaggaagaa aatcatcgtt ttattcaaaa aggagttttt gacaaatgaa
     8341 aaaacgtata ttatttatcg gtgatagcat tacagacgca ggaagagacc gagcagaagc
     8401 aactgatcta ggtaaaggtt atccactttt gattgccgga aaactaatct cccaatatcc
     8461 aacgatggaa tttgaatgcg tgaaccgtgg gataggagga gacaaaattg cagatctgca
     8521 agtacgctgg caagaagatt gtttagcttt acaacctgac tatgtatcga ttttgatcgg
     8581 aatcaatgat acctggcata atactagcga tcaggctata ttcggaactg agaaatctgc
     8641 tcagcaattt gaatccgttt atcgaaaatt actaaatgaa cttcaaaaag cgggaatctg
     8701 caacatcatc ttgatggagc cgtttgtact tcctataacc gacgaccgtc atggctggcg
     8761 taaagactta gaccctaaaa tccaaattgt tcgtaaactt gctaaggaat atggcgcatt
     8821 gctggttcct ttagatggta tattcaatgc tttagggatt gcttatggtt tttcgaaata
     8881 tacaagagaa gatggggtcc acccaacgat tggcggacat gagatcattg cacaaagctg
     8941 gatagatgca attgccgcag tcgatttttt tagtggagat aaaaatgagc gaaagaaaag
     9001 tgaaaaatga tggaaataag gtgggcagta aaagaagatt tacctcaaat caatcagctg
     9061 attactgatc atccagaatc attcgtaaaa gcaaaaacgg cagctgacct aaatgataag
     9121 aataaactat tgctcattgc tgtaacaaaa gaaaaacaga ttatcggatt tgcgcaagcg
     9181 gacgtgcaac agtcagaaga acgattggat cagcgaccat caggaaaact aatccaacac
     9241 acattagtaa caaaagaaaa tacgaaagaa atccaaaaaa aatttgcaga atacttgatg
     9301 ctggaagcta aaaaaagaag aataaaatca atttttgccc agttttaatc aaaaacagta
     9361 atagaaggag aaatacaatg agttgtttag gaaatgtcat ctggtttata ttcggcggac
     9421 taatcggtgg tcttagctgg ttagcagcgg ggatactttg gtgtatcacg atcataggta
     9481 tcccaatcgg tatgcaatgt tttaagatcg cctccctttc ttttgcgcct tttggcaaag
     9541 aagtaattta tcaccagtct ggcatgtcat tgatcgttaa tatcatctgg ctgatcgttt
     9601 caggcctacc aattgctctc gcacatgtcg tcagcggcat ccttctatgt atcacgatca
     9661 tagggatacc ttttgccaag caatctttca aacttgctcg attggcgttg atgccattcg
     9721 gtgcagaaat tgtataaaag agaataataa aggttagata acgcatagag tcaggtggga
     9781 tacatttatt tcacctgact ctatgcgttt tcctgtataa gttcttccat gtgactttta
     9841 cgtcttttat ttatatggga ttactgttta tggaaagttg aaataatttt cattaaagaa
     9901 ctctttctat atttttgaaa gcgtagtgga gcttcgaata tctgggaagg acattttgca
     9961 gggattgaaa aaagatattg tcctatcgaa aaattaatga atttagacaa attgccagca
    10021 acaggcttca aacttagttg ctatcctgtc aagatcaaga aggcaagtgc gggttggata
    10081 agagcagtgg caatgatcga aaagaaaaag aaagaataag ccaaaacatc tgtatggaag
    10141 aaattttccg atgcagatgt ttttcatttt ttctttcgga taccttgaca tatgaataac
    10201 cattcattta taattagtta tgaagagata ttcatatgtt gaggcgagga ggtcattaaa
    10261 atgaaaagtt acagattaga aggtttagat tgtgcgaact gtgcaatgaa aatcgaaaaa
    10321 ggagttcaga aaatcaatgg agtcaaggaa gcaacggtaa acttcacttc tggaaaacta
    10381 acgattgacg cagaggaaga tcatttggct acgattgaac aagaaacaaa aaaagtagtg
    10441 aaggaattag aaccagatgt caaagtaaca gaaatagata aggaaaaagt atctgaacat
    10501 ggaaatgaaa aagagagaaa tacgctgttc cggattttat tttcccttgc aggtatcgct
    10561 ttattgctat tatttgattt taatgaacca attcgcttga tagggtattt attgatttat
    10621 ctgctgatag gatatgatgt agtgaagaag gcagtgatga atatcgtcaa aggaaaaatc
    10681 tttgatgaga attttcttat gtctgttgct acgattggcg ctatgttgat cggtgaatat
    10741 ccagaagccg tagcagtgat gctattttat caaatcggcg agtatttcca aggattggca
    10801 gtcagtcatt caagaaaatc gattcgcgaa ttgatggcga tccgtccaga aacagctcac
    10861 gtgcagacgg cggaaggatt gatgaccgtg aaccctgaag acgtgcagat cggtcagttt
    10921 gtacttgtga agcctggtga acgtgtaccg ttagatggaa cgatcatcga aggagaatca
    10981 ttagtcgata cgtcagcact tacaggagaa tcagtcccta aatctgtata tgttggtgaa
    11041 acagtattaa gcggatttat caataaaaat aaaccatttt tagttcaagt agaaaaatct
    11101 tatgaaaatt ctactatcag caaattatta gaattagtag aaaatgccag cagtaaaaaa
    11161 gcaccagctg aaaatttcat tactaaattc gcgcgctatt acacaccagt cgttgtagga
    11221 ttggccgttt tattagctgt tttacctcca cttgttgttt ctggagcggc gttttctgaa
    11281 tggatctatc gagcattgac atttttagtg atctcatgtc catgtgcatt agtgatttcc
    11341 gtgccactta gtttctttgg cggtatcgga ggagcaagta aaatcggtgt attagtaaaa
    11401 ggttcaaatt acttggagtt gcttgcacaa actgagacag tggtattcga taaaaccggc
    11461 acattaacga aaggtgactt ttcgatccag acgatcgata caaaaatcga tccaaagata
    11521 ttcatgcaat atgttgcgag tgcagaacag ttttctacac atccgattgc tcaatctgtt
    11581 ttagaaggct acgcaggtcc gcttttacca acaactaata tccaagaatt tgcaggtgaa
    11641 gggatcttag ctgaagttga tggcaaacaa gtattagtag gtaatcacaa attgatggaa
    11701 agatttgaaa tctcttttcc ttcaagtcaa gaaatcggta cattgcttta tctagcgatc
    11761 gatcaaagtt acagtggtta tcttgttatt gcagatacct tgaaagaaga tgccgtagac
    11821 gcattggttc aattaaaaca ggctggtgtg aagaacacag tcatgttgac gggcgactcg
    11881 aaaaaaattg ctgatcatat cgggaagcaa gtaggcgtgg acaagatata tagcgaattg
    11941 cttccagaag ataaagtgca gcggttagag gaaatcttac aaagaaacaa taaaaagacc
    12001 gcatttgtcg gagatggaat caacgatgca ccggtgctag caagagcaga tgtaggaatc
    12061 gcaatgggtg gattgggaag cgatgctgca attgaagcag cagatgtagt catcatgaat
    12121 gatcaaccat cgaagattgc agaagcaatc catttagcaa agaaaacatt gaaaatcgtc
    12181 aaacagaaca ttgtctttgc tattgggata aaaattttgg tacttgcctt aggtgctttt
    12241 ggttttgcat caatgggaga tgcagtattt gcagatgttg gggtgacagt attagctgtt
    12301 ttgaatgcca tgcgaagctt gcatgtaaaa aataaacgtt gatcaatgaa gaggacgaat
    12361 gaacgtcctc ttttttttat atccttttct taatgaaaaa tgtaaagaaa atagataaga
    12421 ttataaaata actaatgttt ttaattttgc agaaaattca aaatagcttg aaattcaata
    12481 aggtcaatga aataaaagaa aataacttat ttttttgatg gaaaaa
//
LOCUS       DACDUU010000080        12344 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.080, whole genome shotgun sequence.
ACCESSION   DACDUU010000080 DACDUU010000000
VERSION     DACDUU010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.080"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..154
                     /locus_tag="IWL30_002378"
     CDS             <1..154
                     /locus_tag="IWL30_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP9707104.1"
                     /translation="SLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTL
                     RYSLGY"
     gene            complement(235..1218)
                     /locus_tag="IWL30_002379"
                     /pseudo
     CDS             complement(235..1218)
                     /locus_tag="IWL30_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311512.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(1613..2455)
                     /locus_tag="IWL30_002380"
     CDS             complement(1613..2455)
                     /locus_tag="IWL30_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9707105.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGI
                     PYINNLGLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQH
                     LEIS"
     gene            2780..3196
                     /locus_tag="IWL30_002381"
     CDS             2780..3196
                     /locus_tag="IWL30_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP9707106.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3193..3663
                     /locus_tag="IWL30_002382"
     CDS             3193..3663
                     /locus_tag="IWL30_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9707107.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3677..4450
                     /locus_tag="IWL30_002383"
     CDS             3677..4450
                     /locus_tag="IWL30_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9707108.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4464..5276
                     /locus_tag="IWL30_002384"
     CDS             4464..5276
                     /locus_tag="IWL30_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9707109.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5330..6295
                     /locus_tag="IWL30_002385"
     CDS             5330..6295
                     /locus_tag="IWL30_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9707110.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6432..9155
                     /locus_tag="IWL30_002386"
     CDS             6432..9155
                     /locus_tag="IWL30_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9707111.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            9168..9800
                     /locus_tag="IWL30_002387"
     CDS             9168..9800
                     /locus_tag="IWL30_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9707112.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            10005..10232
                     /locus_tag="IWL30_002388"
     CDS             10005..10232
                     /locus_tag="IWL30_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HAP9707113.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            10301..10567
                     /locus_tag="IWL30_002389"
     CDS             10301..10567
                     /locus_tag="IWL30_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP9707114.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            10557..10826
                     /locus_tag="IWL30_002390"
     CDS             10557..10826
                     /locus_tag="IWL30_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP9707115.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
     gene            complement(11005..12306)
                     /locus_tag="IWL30_002391"
     CDS             complement(11005..12306)
                     /locus_tag="IWL30_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9707116.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         4034 a         1999 c         2421 g         3890 t
ORIGIN      
        1 ttccttagaa aaattaggtt gtcaaaattt caactcacgt tctgttaaag ggctagagag
       61 tagatttgaa agcatattac tggcttacag tgttctatta agtcgagcac aacgacgttt
      121 tgaaggaact ttgagatatt ctttaggata ttaaaattaa ctagcaccac gggtaaaata
      181 aatagttttt ctatcaagta tattgaacta cgataaattt gttattttgt tttgtttaat
      241 ttcgttgtac ttattggctt tgacttcgat ttctttaaat actgggtaat cggtgtccgg
      301 caaagctagc aaatctgttt tttctgtgtt ccatagatca ttgataatga catgtttttc
      361 gtaatggatt cgctctcgat cgtgtgacag ctccaccgct aatgcctcgt tgaaactggc
      421 gaaatccttc ataatcggtg tagatgaaaa gaagttgtac cgtacatagc cgactttgtt
      481 ttccatacta cctttctcgt ttccgctacg tggattgcac acctgtgtct caaaaccata
      541 gtgcatagaa aatcggagga aaccgtcagt cagcaccgct tgttcagtct ttgtttttgt
      601 ctaagtaacg gccgttgaca tattatcaat ccgtattttc cgaggaacgc cacctgattg
      661 gctgaagagt tctttcatgc cttccaacaa acattcttga ttctcggcgg ggagtacaac
      721 ggcgaatcct gcattactaa aagggaaagt catcactaat accttcacat ctttaaacgc
      781 tccatcgtga caaacttcca tcgtaccaaa atcaagttgc gcttctgctg gcgggtgttc
      841 aagacgctca aagccttggt cttccagtgt ttccaagtag tgtgtcgctt tccaatcttg
      901 aatgaataca cagactgttc ggtaggatcc tggaaagcca tcggctttta aagcagtagt
      961 cattgcttca gcagtcctac gctttttatt tggcagctta gcatcttctg aaagccatag
     1021 ggaaacgatg cctccccact tttctgtata catcatacca gtctttttgc gtgctttagg
     1081 ttgcggcagc aaatcatcat ctgcatattt tttagccgtg cgccaattta catctagctc
     1141 ctttgcaata tgattaatgg atttatcatg attatttcgt aaatttctga tagtattaat
     1201 atcggtcatt gtgagcatac ctttcttacc tccagttaca gtgtcgagac tataacttta
     1261 gaggagtttc aggttgcccg caatggcttt ttatcttaac ttaatcgatt tgcggagttg
     1321 tgtagttttt cgttgcactt gtatgtactt ttattctgcc ataaacagct taatgaatgt
     1381 ccacttgaaa aagtgaataa acgaaataga tgactcgatt taataaggaa gtaaacccac
     1441 acagaaatat aggtgatttt ccttgaatga ccaagatatt gaaagttagt gaaaaaacac
     1501 aaaaaggtag ccctattcac agaaggtctg ccttttgtgt tatcgccagc aaacattaga
     1561 gggggtattt catatagctt ttgtcctctc gacagtccta tcagtttctc ttttaactta
     1621 tctctaagtg ctgtccagtt ttaatagctt ctagcttgtt tcctaactgt gtttttaata
     1681 tgttgaaggc ttttgaacct gtgcagtggc aagtaaggat tttttctact cgtgagtcat
     1741 tcaatttatt tccaattttc attacctctt gctcaggtag acctaaatta tttatataag
     1801 gtatgccaat catatggaag ccaccaatta ggtattttat gggtttagtc ggaaatgttt
     1861 caagagcaat ttccatcata tcgataatgc catgatgact acagccagta aagacaatca
     1921 gaccgtcttc ttgctcaatt acgaccaata attcatctga aaaatcatca taaacaaatt
     1981 tatcatccat ctttttgtat aaattcttgg tggtcgagtt tgctttgcct agggggatga
     2041 tatggaagct cggagcaagt tctgttttcg tttttattgt atgtaaattc tcatgcattt
     2101 taaggatttc atttttagga ataccaacat acagaaacgt ggttaaaatc tttaaataat
     2161 ggggtaaagt tacaccttca gctgtataaa taggggcgtt tcctgatttt aaatatctta
     2221 acccgccggt gtggtcagaa tgaccgtgtg aaataatcac tgcctccaga tcattcaaag
     2281 tttcccccat tttagtcgca ttatagacta attttttatt agcgccagca tcgaagagat
     2341 atttcttccc tgagtagtta attaagagag ataacccatg tccactttta aagttcaaat
     2401 ttattcttga gttttcaaat aaaaccttga tttccactat aacatctcct ttcacatgaa
     2461 cgtgtattca tatattgtag cacattaagt tctatttttt gttgtaatta ccgaaaaaaa
     2521 tattaatgga cgctttttac gctttatttc gtatgtggaa gaaaatttct taggatgtgg
     2581 cgggggattg gggcaaagct aaattccaat tacagtgatc atttaattag acacctttaa
     2641 atacccacac tttcatcaac actataattt agggaaagaa aagtgttggc atgatataga
     2701 aaaaccttta attaacagcg tttcctaaaa ttggcacgca tcttgcttat agaaaagcgt
     2761 aaggatagga ggaatgaaaa tggcaactat aattcttgcc agccatgggg aatttgctca
     2821 aggattaaag caaacagcga caatgattat tggagatagt gtaccaattc atgccttgtc
     2881 agcatttaga gatgaagatg aatctatttt ggtacaaata aaaaaacttt tagccacaat
     2941 agatatagaa gaaacctaca ttttaactga tattctaggt ggtagtgtga ataatgatat
     3001 gctcactatt attaaagagt atgagggatt gcaattaatt actggaatga acttaccttt
     3061 agttatttct attgcaacct ataatggaaa aattggggaa agtgagctag aaaagcttat
     3121 taaagagagt caacaatcag tgattgattg tagaaaatta ttagtagaac aacttactgg
     3181 aggagatgac ctatgattaa gttagtacgt attgaccatc gtttgttgca cggacaggta
     3241 gtattttctt ggagtaaatc attaaatatc aatcgtatta ttattgccaa taaagaggca
     3301 gcgaatgatg aattcaaaaa aatgtctttg aatttatcta aaccgactgg cattaaatta
     3361 acaatttttt cagttgaaca gttactagaa aaaatgccta aaatcgaaac acttaacgaa
     3421 aatatcatgc ttatttttgg taatacaaaa gaaattcgtc aattttgtga agcctatcct
     3481 aaagtacaag aaatcaacta tggagggata atcaaaaaag aaggttccaa gcaatttagt
     3541 aatgccattt tcttgaatga atctgagcaa gaagatgcca aaaaattaaa agctcttggc
     3601 attaaacaat ttattcaaca ggtaccaact tctaaaaaag aagatttgaa tacaatgtta
     3661 taaaggagga atgaaaatgc tcgttcagtc aattggttta gggttaatcg gtgttttttg
     3721 tattttagac tcaaggcttt taggaagaat gaattttgaa cgaccgttga ttgtcagtac
     3781 gcttgttggt ttggtacttg gagacatgga aaaaggatta attgttggag cctctttgga
     3841 attgatgtcc ttaggattag taaatatcgg agctgcttca ccacctgata tgaatatggc
     3901 atcaattatt gctacagcct ttgccatttt atcaaatgcg aatgcagaag cagcattaac
     3961 gattgccatt ccaatttcag tgttaggtca aatactaggt atcttaatgc ggacgatttt
     4021 gtcaaatttg actcacgttg cggatcgctt tatcgaacaa ggaaaattta gccaagcaag
     4081 gagaattcat atactttatg gctcgggatt atatgctctc atgtatttta ttcctatttt
     4141 tctagcaatt tattttggaa cgaatcttgt ttctcagatt gttgatgcga ttcctgagtg
     4201 gctaacaaat ggcttaacgg ttgcaagtaa aattttacca gcatatgggt ttgcgttgtt
     4261 attacaaacg atgttaacga aaaaaatggt gccattttta ttacttggat ttttaattac
     4321 tgcttattct ggattgggaa tcattggtat tgcagccttc gcatgtattg ttgcgtttgt
     4381 catggttcaa gttcaaggta aaaactcaaa tggtaaccaa aatgagccag atccattaga
     4441 tgacttataa ggaggtagat taagtgaaag agaaagatca aaatgcatgg aaatatcggc
     4501 agttcttatg gcgttcatgg gcaattcaag cctcatggaa ctatgaacga caaatgaaca
     4561 tgggttttat gtacggaatt gcaccaattc tagataacat ttacaagaat ccagaagatg
     4621 aagcaatgaa gaaagaagct tacgagcggc atatgatgta ctacaactgt acccctcaaa
     4681 caagtgcatt tgtgttaggg ttaactgctt ctatggagga gcaatactac gaagaccaag
     4741 aaaactttaa tccggaatca atcagtgcag tgaagacttc tctgatggga ccgctatctg
     4801 gtgttggtga ttctttcttt caaggaacga ttcgggtatt agcctttgga ctaggaatta
     4861 atttagccca acaaggtagt attttaggtc caattctagc aatgttaatt tcctttgtac
     4921 cttccttttt agttacttac tatggtggga aaattggcta taacatgggg aataaatact
     4981 tgtctaaact gtatcaagaa ggtttgatgg ataaagtaat gtatgtttgc tccattgtgg
     5041 ggttgatggt cattggttcg atgattgcca gcatgatcgg gattacaaca ccaattcaaa
     5101 tgggtaaagc ttttgtctta caagatgtat tagatagtat tatgccacaa tttttaccat
     5161 taggattaac cttcttcatg tactggttat tgcagaaaaa ggtcaaaaca ggatggatgt
     5221 tggcaatctg tttagtcgga ggaattattt ttagtttgtt aggtatctta caataaggga
     5281 ataaccaata aatttggttg aataatataa ataaggagga attcaattga tgaattcaaa
     5341 agaaattgtt aaagaaatcg tagcgaaaaa agaggtaaaa tcaatcgtat ttgttggctg
     5401 tggcgcttca aaagcggacc tttatcctgc aaagtatttt ctagaccaaa atgcgaagca
     5461 gttacggatt agtcattaca cagccagcga atttaactat gcaacaccag catctgtaga
     5521 tgagtcaact gtcgttattt cagcttcttt aggaggtacg actccagaaa cagttgaagc
     5581 taacgcagta gctaaagaaa aaggtgctac agttattagt ctaacacgaa gcattgactc
     5641 accgttaaca aaagatgcag attatgtaat ttatcatcga tttgcagaga actatgctgc
     5701 taaattagaa aaaacaggct actgtttaga attggcagta gaacttttac atcaagtaga
     5761 aggttatgac cattatgaag caatgcaaga aggatttgct actatctatg atcttgccca
     5821 aaaaagtgca gaatcagctc gtaaagatgc gaagaaattt gctgaacgct ttaaagatga
     5881 aaaagttatt tatgtaatga gtagtggtgc tacacatgaa gtagcatact caacatcaat
     5941 ttgcttgatg atggaaatgc aatggattaa ttctggtacc ttccattcag gagaattttt
     6001 ccacggacca ttcgaaattg tggataaaga cgtaccgttt atattattaa tgaatgatgg
     6061 aaaaactaga aatatagatg ctcgagcatt aactttcctt gaacgctttg atacgaaaac
     6121 agaagttgta gatgccttgg attatggctt gtcttcacat attgctaaag aagtcttaga
     6181 ctactttaac ccattcgtga ttactgcagt atttagagtt tatgcagaag aattgtcaga
     6241 agcaagagaa catccattaa ctaagagacg ttatatgtgg aaactaactt attaaaaaat
     6301 agatcaaact aaataaggtg gcttgagaaa ttaagccacc ttgttttctt caatgtgatt
     6361 ttatacatat ggtaaaataa ctagtacata tggtaaaata actagtacat attgtgaaag
     6421 caggtggcaa gatgcagcga ttaaatgact atctttatca actaacattg cttttgaaat
     6481 ctacgccttc agataattat tcagctcgca gtatttcgca agctctacag ttgaatagaa
     6541 gtacaatcag tagctactta aatgaaggtg ttcgtgaagg gcttttcata aaagtaaaaa
     6601 gttacccggt tttattttta catagaacag ctcttgaaga actacatatc acactgaata
     6661 actccgaaat agaatcaatt gaaagcttgt taacaatttc tcaaaaaccc gcattagatc
     6721 aagtaattgg ctctaaagga agtttgaaag aagcaattga tcaaattaaa acggcagtct
     6781 tatatcctgg aaaagggttg cctttattat tgatcggcgc tagtggatca gggaaaacct
     6841 ttctagcgaa taaaatatat gaatatgcag tagaagaaaa ggtcattcac acatcggcac
     6901 ctttcataga ttataattgt gcacaatatg tgagcaatcc agagttacta tcctctgctt
     6961 tatttggcta cacgaaagga gcatttaccg gagcaagcca ggagcatact ggtttgctag
     7021 aaaaagccga tggaggagta ttgtttttag atgaagtgca tcgcttatct gaagaagggc
     7081 aagagaaatt atttacattt atggatacgg gtgaattctc tcctatgggg gataatagta
     7141 ttcggaaaaa agccgatgtt cgattagtct ttgcaacaac agaaaatata tatacgactt
     7201 tcttaccaac ttttcttagg agattgccag ttattgttaa tctacctaga tttcaacaac
     7261 gtccgtcgtt tgaacgtctg tcattgattg atgaattttt tgtttctgag agccaaattt
     7321 tagctaagga actatctgtt tctgatgcat tgattcattt cttaatgaat agtgattttg
     7381 aaggtaacgt ggggaatatt aaaaatatta tcaagtatgc ttgcggaaac gcatatatcc
     7441 accagaagag tcagcaaaca attcaagtta aattattgga tatgccttta gagtacagtt
     7501 ataaattcaa ggagcaacta aataaaccca gaaagaaaag agcagatcga atctactatc
     7561 cagaagaaac acgacaagtg caactagaaa gaaacaatca gctttttgtt caattttttg
     7621 accaattact tactggattc tctgaagtga ttgaaaataa caaggatatt aaactatttt
     7681 tagaagaaat ggttaccaag gtaacacaag taatggatac gcttattttt caagaggatt
     7741 acgaaaaaga gcagtcttta tacgccattc taaactatca tattcgtcaa agtattgatt
     7801 ttatgcgaga aagctacggg tttgaacaag atggtaaccg ggtagtcgct ttggccaact
     7861 atctatatgt caaggataat caagtgattt ttgaaaatag tcttcaagaa aagaaaacca
     7921 aagatcagct taacgaattt tttgctaatg ttcttgaaac cccttattgg tatgcaaaaa
     7981 aaatcttaag ccttttatct gagtgtttag atcaacctgt gtatgaagag gacattttct
     8041 ttattacgct atatttttat agtttgaatg tcacgaatgt taaaacaggt attaaaagta
     8101 ttgtgctagc tcatggttat tctacagcta gtagtatggc gaatgttgcc aaccgtatgc
     8161 tgaataagaa cgtttttcaa gcttatgata tgcctttaga tattacatta gagaaaattg
     8221 aagcaaaatt gattcaattc cttggagatt atcgaacaga ttcaggcttg attttattgg
     8281 tagatatggg ttcactaaac caactaggcg gtttattagt gaatcatcta aatggaccac
     8341 tgttaatcat tgatcatatc aatacaccgt tattattgga agtcggccaa caagtattaa
     8401 acaataaaag tatcaccgaa ataggagcag aaattgaaac aagtcttact ttcaaaaagc
     8461 agttactact tcccaagaaa aagaaaaaga aagccatcat tacttgttgt tatacaggga
     8521 ttggtagtgc gacccagatt caggagattt taacgaaatg cttgaaagat gcagttagag
     8581 aattagtaat tattccgtat gattacaaaa agctgttaga aaacaagaat tttgagactc
     8641 cttttcaatt atacgatgtc atcatgattg taggtacaga agatcccaaa attaatcaga
     8701 ttccatatat tggattagat cgcctgatta atgggggaag cattggcgaa tttgttcatt
     8761 acttggaaaa agagctgact attgaagtga acgatttaca aaaagaatta attttcaatt
     8821 tttcaattag taaaattgtc gaaaatttaa ccattttgga tccagataag gtgctatctt
     8881 ttgtacaaaa agcaattaaa gaattatcga agttattaaa ttttgatttt tctaataatc
     8941 agctgttttt gttgtacctt cactgtagtt gtatgattga acgaattgta cgtaaagaaa
     9001 gtgtggatga gcaagaggac attgaagaat atatgcaagc aaatggagaa ataatggata
     9061 aaattcatag cgcttttttt gagattgaaa gggaatacac aattgaaata ccagatttag
     9121 agttgcgatt attgagcgat attgttcaag gataggagtg aattcgaatg acaatgggag
     9181 taatttttga tatggatggg gtactcatag atagcgagac attttatttt gagagaagaa
     9241 tgaacttctt tcaagaactg gagatagatc caggatcaaa ccagattgat gattatgtgg
     9301 ggaaaacaga agctgcaata tgggaaacat tagttcctga ggacaaatca ttaagagagg
     9361 aactttatcc gaaatatgta gattatcgaa aaacgcatcc aattgatttc gctaaagctt
     9421 tacgaaaaga agtgaaaccg ttacttgact tcctcaagaa gcgggatata ccattggcac
     9481 tggcatcttc ttctccagct gtcgagatag agagtatgct cattcaatgt gaacttactg
     9541 aatatttttc ctttgttata agtggtgagt cattaaaaca aagcaaacca catcccgaaa
     9601 tatatttaaa agcgaaacag caattagact gtgacttctt tattgcagta gaggattcaa
     9661 cgattggtat taaatcagca aaggcagcaa atatctatac ggcggcacta aaacaaccaa
     9721 tgcaaatcga tcagagtgaa gcagacatac aaattgaaag tctacaagaa ctggaacaaa
     9781 ttgttttaga aaaaggttag tgaattaatg gaaataaggc tatatatctg tgctagctat
     9841 tttgactaac caaaaaaact tgctataatt caggtgtagt aaaaggtgct ggtaacacct
     9901 tttacgaaaa tgcttcggca gtgataatgg gattggcttt ttaagttttt tattttgttc
     9961 gcaatcccta aggtcatgtg aatgatccta gggattgcta gaatattttg caacagaacc
    10021 aaaatacgaa tagttatcaa gcagccatat tgactgcggt caatttagga ggggatactg
    10081 atactactgc tgccgtaact ggtgctcttg cgggtattct ttacgggggt gaagtgatcc
    10141 ctgaaaattg gagaaaatcg cttcaaaatg accagcttct taaaacgatc agtcagagat
    10201 tttgcgatac cgtgctatct caaagaattt gaataatgga tatgatgcaa atcttgtttg
    10261 tactaacgtt gtattgacaa aaaggatttt atttagtaca atgttagtac aaatgatgaa
    10321 cggaggtaga atcatgtctc aatcaaccat tacatttagg attcccgacg atgaaaaaga
    10381 actggtatta gaatatgcaa aagcacacaa ttcttcattg accgaacttt atcgaaatgc
    10441 tgtactggat aaaatcgaag atgaaatcga tctacggact ttacggagtg caattgaagt
    10501 ttccaaagag aaaaaagaga gtggtatttc tcaagaggaa atggagaaac tgctaaatga
    10561 attataaagt catctacatg ccgcaagccc agaagcaatt aaaaaaaatg gacagaaacc
    10621 aagctaaaat aattgtcgcc tggatcagaa agaatttaga aaacacgact gacccgcgtc
    10681 agcacggaaa aggtttgact ggaaataaga gtggggaatg gcgctatcga attggaaact
    10741 atcgaatatt ggctaatatt tgtgattctg aaattgaaat ccagatattc tcaattggac
    10801 atagaagtac aatatataaa agataaccgt ttttggaacg aagtagagtt tcgaaagtca
    10861 atgtataatc ttttgaattt tgcataatac cacaggtaga aactacccga tactatcggt
    10921 tggtttctgc ttttttatgg ctctttgtca aataggactg atagagtgag ataagaacat
    10981 ctaaggtaga attccttttc tactttagat gttttttgat tcataggtaa tcagattatt
    11041 tagtaagaaa attctggtct tcattttccg aaagcttcga aagccataag cgactcgttt
    11101 caatgctttg atgtgggtat gcagattttc tattttggca tttgaataag gcaattcgag
    11161 ggctaaacga atgccggatt catagtttat aaaagctttt tttatagaat gcagttcttt
    11221 tggaacggaa ttgggcatcg ttcttacaag tttaaagaaa gtgtccgcct ctttgtcgtg
    11281 aaacgcttga agaatttctt gatagtaaaa ataggcgatt cgtaattcgt cagagatcga
    11341 aagcagacga tccaccactt cactttcggt gagatacgcc caaccaaaat tgcgccattt
    11401 ggtccatttg tcataaacaa gcttgtcctg tcgtttgatc agaagccgag acaacgcctt
    11461 taattgtcga taatatttgg cttgtgttgg atcgtttcga tcaaagcttt tcatgattcg
    11521 tacgcgaata tcctcaaatt tacgaatgag atgtttgaca atgtgaaaac gatccacaac
    11581 gagttttgcc tttggaaaac attcctttat cagagaggca taagaaacat tcatgtccgt
    11641 cacgatgacc tttaccttca atcgtgcgcg tcgttcaaat tgcataaaat aagaaacgag
    11701 agcctttttc ttccgtgaag gaagaatatc cagtaatttt ccagtatcgc catccataca
    11761 agaaaaggag tagggaccga cttgattttt cagtgaacga aattcatcca ttaataaaca
    11821 acgcggtaac caatttcttt ttacttttgt catggggatc agagaagcga gtgtccgaga
    11881 aacggtcgac caagaaacgc cgcaatgctt agcaattgtc gtcattgctt gtttttcagt
    11941 tagctcttct aaaattttat agcgcacttg tttagcgata aagcagtttc tatcgacaat
    12001 gtaggtattc gccgtaaaat aggtgtggca gccacgacag tggtagcgtt gtttcgctaa
    12061 agcaagataa gtcagttggt tcgcacattt attaagttga atgagcgatt ttttctttcc
    12121 gttcttaacg atcatgcctt catgattcat ctgtttacac aaaggacagc gacgaatttc
    12181 tttacttaac gtcgcagaaa caacaagact cttgatattc ttaatgctct tcgattccac
    12241 atttgtgatg aaaatgtttt tatctttgat tccaagtagt agtttgatat gatgaagatc
    12301 aggcatacag aacgctcctt tgattgtgtt gtggtgattc aatt
//
LOCUS       DACDUU010000081        12305 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.081, whole genome shotgun sequence.
ACCESSION   DACDUU010000081 DACDUU010000000
VERSION     DACDUU010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.081"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(178..735)
                     /gene="frr"
                     /locus_tag="IWL30_002392"
     CDS             complement(178..735)
                     /gene="frr"
                     /locus_tag="IWL30_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAP9707117.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(738..1460)
                     /locus_tag="IWL30_002393"
     CDS             complement(738..1460)
                     /locus_tag="IWL30_002393"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAP9707118.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1596..2477)
                     /locus_tag="IWL30_002394"
     CDS             complement(1596..2477)
                     /locus_tag="IWL30_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAP9707119.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2576..3358)
                     /gene="rpsB"
                     /locus_tag="IWL30_002395"
     CDS             complement(2576..3358)
                     /gene="rpsB"
                     /locus_tag="IWL30_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAP9707120.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3717..4196
                     /locus_tag="IWL30_002396"
     CDS             3717..4196
                     /locus_tag="IWL30_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAP9707121.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4413..5504
                     /gene="phnW"
                     /locus_tag="IWL30_002397"
     CDS             4413..5504
                     /gene="phnW"
                     /locus_tag="IWL30_002397"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAP9707122.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5497..5625
                     /locus_tag="IWL30_002398"
     CDS             5497..5625
                     /locus_tag="IWL30_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP9707123.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            5629..6174
                     /locus_tag="IWL30_002399"
     CDS             5629..6174
                     /locus_tag="IWL30_002399"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP9707124.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6628..8319)
                     /locus_tag="IWL30_002400"
     CDS             complement(6628..8319)
                     /locus_tag="IWL30_002400"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAP9707125.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8739..9686)
                     /gene="arcC"
                     /locus_tag="IWL30_002401"
     CDS             complement(8739..9686)
                     /gene="arcC"
                     /locus_tag="IWL30_002401"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP9707126.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9801..10820)
                     /gene="argF"
                     /locus_tag="IWL30_002402"
     CDS             complement(9801..10820)
                     /gene="argF"
                     /locus_tag="IWL30_002402"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAP9707127.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10911..12140)
                     /gene="arcA"
                     /locus_tag="IWL30_002403"
     CDS             complement(10911..12140)
                     /gene="arcA"
                     /locus_tag="IWL30_002403"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAP9707128.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3376 a         2542 c         2210 g         4177 t
ORIGIN      
        1 ttaaaagatt cccactatct taagtattat agcaatatct aactgttttt cctatgaaat
       61 cattgttttt ttatttaaaa actgaataat tttttctttt tttaattttt aaataaaaga
      121 aatataaata aaaacagagg aagcggaatg atccgcccct ctgtttttat gattttatta
      181 aacttccaat aattcttttt ctttatcagc agcgatctta tctacttctt tgatgctgtt
      241 atctgtcaat gtttgtacgt ctttttctaa tccgcgcaaa tcatcttcgg taatatcacc
      301 atttttttgt tgttttttat attcatcgat cgcatcacga cggatgttac gaatcgcgat
      361 ttttgcattt tctgcttctt ttttcacatc tttagctaat tcttttctgc gttcttctgt
      421 cagttgcggg ataactaagc gaatgacatt tccatcattg gttggactga ttccgatatc
      481 acttgccata atagcttttt caacatcttg aagaatgctc ttgtcaaaag gtgtgatcat
      541 taaaacacgt gcttctggaa tttggattga tgccatttgg ttcaatggtg ttggcgcacc
      601 gtagtagttc acagtaatac ggtccaaaag gctagcgttc gcacggcctg cgcggatttg
      661 cccaagttca cgttgaagat tttgtgctgc ttttttcatt ttttcttttg cttctgtcat
      721 aattgcatct gccattttta tttccccctt acagttgttc cgatattttc gcctaagatt
      781 gcacggcgaa tatttccgtg ttcatttaaa ttgaagacaa gcagtggaat atcattatcc
      841 atgcttagtg aacttgcagt tgaatccatg acttgtaatc ctttggcaat aacttccaga
      901 tgagtcagtt cttcaaattt tactgcattt gcatctactt tcggatcagc agaataaaca
      961 ccgtctacat tgtttttagc catcaggata acatctgcgc cgatttctgc cgcgcgtaat
     1021 gctgctgttg tgtctgtcga gaagtacggg ttgccggtac cgcctgcaaa gataacgatc
     1081 cgtccttttt ctaaatgacg ttctgcttta cggcgaatat atggctctgc gatttgacgc
     1141 atttcaatcg aagtttgcac gcgtgtcggt acacccacgt tttccaacgt atcctgtaaa
     1201 gctaaggcat tcataactgt agctaacata cccatgtaat cagcttgagc acgttccatg
     1261 cccatttgtg ctccgatttg tccgcgccag atatttccgc cgcccacaac gattgccatt
     1321 tcaacgccca gctcgtgaac ttctttgatt tcttggatga tttctttgat tactggtggt
     1381 ttgattccaa atcctgcgtc cccagctaaa gcttctccac tcaattttaa gactacacgc
     1441 tgatatttcg gtgtgaccat gttcattcct ccaatatttt ctattgccat tttaccatac
     1501 ttagatgatt tcgacactct aagactggtt tttcttgttt tgcttaaaaa aagggagcgc
     1561 acaaaccgtg cgttcccgtc tgttattcaa cagaattatt ttttcacttg gctcataact
     1621 tcttctacaa agttatcttc gcgtttttcg ataccttcgc caacttcgaa acgtacgaat
     1681 gttttaacag ttgcgccttt tgatgcaacg tatttttcaa cagtcatatc aggatcttta
     1741 acgaatggtt ggtccaccaa agcgatttct gctttgaatt tcttcaagcg tccttctacc
     1801 attttttcaa cgatattcgc tggtttgcct tcgttcaatg cttgttctgt aagaacagtt
     1861 ttttcatgtt ctaattcagc ttctggaatt tgtgattcgt ttacataacg aggattgata
     1921 gctgctacgt gcatagctac gtctcttgca actgtttcgt cagttgttcc atctagaaca
     1981 gtcaatacgg caatacgtcc gcccatatgc aagtatccac caaatgcagc attgtcgtct
     2041 ttttctacaa cttcaaagcg acggaagctg attttttctc cgataacttg agttgcttcg
     2101 atcaagtcag attcaattgt acctttatct gttttgattt tcattgcagc ttccatgtct
     2161 gctggtttgt tttcagcaac aagttctgca atttctttta ctaaatcttg gaacatttcg
     2221 tttttagaaa caaagtctgt ttctgagttt acttcaacga ttgctgcagt gttacctttc
     2281 acagcaactg aagccaatcc ttcagctgca atgcggtcat ttttcttcgc agcctttgcc
     2341 attccttttt cacgtaaaag atcaactgct ttttccatgt cgccttctac ttcaactaat
     2401 gcttttttcg catccatcat tcctacgcca gtcatttcgc gtagttcttt taccatttta
     2461 gctgtaacgt ctgccatgtt taactcctcc tgtgtgtttt tctttttaaa aaaagctgct
     2521 tcaaaggaga gtgaggcttg tcgcccagcc tttgaaacag ctccatcatt caaaattatt
     2581 ctgctgaaga attgtcgcct tcaacaacat ctacgatttc ttcgattgat gttgctgtgt
     2641 tttcttctgt gaaatcttct tcaactactt gatcttcacc ttggtttcct tcgatgaaag
     2701 catcagccat ttttgaagta atcaatttaa cggcacgaat tgcatcgtcg tttgaaggga
     2761 ttactacgtc gatttcatct ggatcgcagt ttgtatcaac catagctacg attgggatat
     2821 ttaatttgtg tgcttcttga acagcaatac gttctttacg tgggtcaacg atgtacatta
     2881 catctgggat tcttggcata tcagcgatac cgcctaagaa tttttcaaga cgttcacgtt
     2941 ctttgttcaa accagcaact tcttttttag gtagaacttc aaaagttcca tcttcttcca
     3001 ttgcattgat ttgtttcaaa cggctgatac gtttttggat agtatcccag tttgtcaatg
     3061 ttccgcctaa ccaacgatgg ttaacgaagt attgtcctgc acgagttgct tcttctttga
     3121 ttgcttcttg tgcttgtttt tttgtaccta cgaataatgc tacgccgcct tcttcagcaa
     3181 cgctcttcat gtaatcataa gctgcatcta ctaatttaac tgttttttgc aagtcaatga
     3241 tgtagattcc gtttctttct gtgaagatat atttcttcat ttttggattc cagcgacgag
     3301 tttggtgacc aaagtgtacg ccggcttcta gtaattgttt catagaaatt actgccatgt
     3361 ttgtttcctc cgatttggtt ttatttttcc tcttcttatc ttctaccttg ccggcaaact
     3421 ttttcaagca cctaccgaca atccaataag aatgtggatt tgctgatgca taaagcaccc
     3481 tcagctatta tacagacaaa agatccgtat cgcaagagca aatatacttt tctgctcaac
     3541 attcaatagt ttacctgaaa gataaaaaga attcaagaaa aacagtagat ttctctttgt
     3601 ttctttatta tcctttttcc ataaaataat tttaggtata aaagacgaat attattgggc
     3661 aatccttctt ttttttgtta tgatacatgt gaaatctttc gtaaaggaga aagaaaatgc
     3721 gcaaagctca aagacatttg ctgattaaac aagtcattga agaattcccg atccgtacgc
     3781 aagaagaact attaaacaaa cttgggcatt atggcgttac cgccacgcag gcaacgattt
     3841 ctcgagatat ccgtgatcta aaaatcgtga aggcacctga tgaaaatggc gtttcccgct
     3901 tcgtattgtt tcgcgaaaaa ggaactcctg agactaggac tgaggaagag gatcggctgg
     3961 ttcaaatgat tgaagacatt gtattgaaag tcgaacgagt ccactttttg acgatcgtca
     4021 ataccttgcc agacaatgct catttatttg catctgtttt agatgagatc aaacctccct
     4081 ctattgtcag tacaattgct ggattcgata cgatcatggt catctcggaa aatgaggagg
     4141 acgcaaaacg agtagaagaa tttcttcacc atccaactga agtatcactt ttttaatcag
     4201 agcacgtatt cctaatatgg aatgcgtgtt tttttatttt caatattttc cttattgaat
     4261 aaacctttac actctttaca aaaatacatc attttttatt caaatttttc aacaccttga
     4321 ttgaatcaca ataatcatca tttatattgg atgttaacaa gagcagcttc ttgctctttg
     4381 tgtatttcaa aaaagaaatg aaaggaaatc atatgtataa attattaact tctggaccat
     4441 tgacgacttc tgaaacagtg aaaaatgcta tgctgatcga tcattgtact tgggatcaag
     4501 aatacaaaga aattacacaa tggataagaa aaaaattatt ggatcttgct caagtatcgg
     4561 aaggaatata cacaacggtc ttaatgcaag gtagcagctc cttcggggta gaatcggtat
     4621 taagttctgt gatcaggcca gaagatactg tactgatttg ttcaaacggc gcatatagac
     4681 aacgaatgat caacatgtgc gaatgtctga aactgaacta taccgtttat gaggtatcgg
     4741 aacatcaaat cctccaagct catgtgatta cacagctgat tgaagagaat ccgtctatct
     4801 ccgcactagc aatggtccat agcgagacta cttccggtat tttgaataat ttggaagaga
     4861 ttgctgctgt cacacaagaa agagatcttt tgtttatagt ggacgcgatg tcaagctttg
     4921 gtgggatacc gatttgtgta gccgaactag ggattgattt tttgatttcc agctcaaaca
     4981 aatgtattca aggtgttcca ggcttttctt ttgtacttgc taataaagcg gaactggaaa
     5041 aacgaaaaaa tgttagccga agtttgtcac ttgatctata tgaccagtac cgcgtgatgg
     5101 aaaacgagaa tggcaaatgg cgtttcactt cccctaccca tacggttttg gcttttcaac
     5161 aagcgctgaa ggaactagag gcagaaggag gaatcgaaac tcgatattca cgttatctcg
     5221 ccaataatca tgtgctacga caaaaaatga cagagatggg gttccagcct ttcattcaag
     5281 aatatcaggg gccattcatc acctcttttc tttaccctaa ggactggcga tttgatttta
     5341 atcattttta tcagtattta aagcaaaatg gatttgtgat ctatcccggg aaaatcagcg
     5401 atcatgaatg ttttcgtatc ggaaatattg gcgagatcta tccggaagat attttggaac
     5461 tgacagcaat tatcaaagac tataaggaga acgttaatga ttaaaactgt aatttttgat
     5521 tgggcaggaa caactgtgga ctttggctgt atggctcctg tccacgcttt tagaaatgct
     5581 tttctagaaa aaggaaccca actgactgac aaagaaatcc gctaaccgat gggaaaatta
     5641 aaatgggatc atatccaaca gcttctttcc ctcccttctg tcaaaaaaca atggatagct
     5701 atttatggac aactacctca ggaaaaagag gtggaggaat tgtatcaaca atttgaacat
     5761 tatctgttta aagatttggt caagcattct accttgaaac ccgacacatt ggaaacgatc
     5821 gaacggttga gaaacgctgg gatcaatatt ggttccacga atggatttaa tgctcctatg
     5881 atgacaatcg tagcagaaac cgcaaaaaaa gaaaggtatt caccagactt catggcaact
     5941 tttgaagacg tcgaagggta tggtcgaccg tttccttata tgatccataa aaacatgcag
     6001 tacttcaaaa acaagtcggt tgatgaagta atcaaagtcg gagatacggt ctcagatatc
     6061 caggaaggga aaaatgccgg agtgttgaca gtaggtgtaa ttgaaggtag ttcgctaatg
     6121 gggatttctt atgatgagta ccaaaaatta gagatgggga aaaagatcgt ttaaaacagt
     6181 ctgttgctct tagattccaa caagccggcg ctgatatttg tgtaaacaat ttaaaagagt
     6241 taacagaata cattttgtac gctgacaaga atccagaatg acttttatac aaaataagag
     6301 gttgtgaaaa agctgtcctg tatcaagtaa taaaaacagc caagcaaaaa tagctcctca
     6361 tatttttcgt ttggttaggc ttattaccgc agatgcaagc ttttgaacac cgtttattcg
     6421 aaaaaaaaaa gggagctgtg acacgacttt tgtcacagtc cccttatttg caaacaaaca
     6481 gtgtttaaga gacaacctct ccactaaacg gtgttcattc agctgactct cggtatttgc
     6541 tcaaaaaacg ataaaatttg aaaagctatt ttctcttttg tttctctaat actcggatca
     6601 gaacgctgat ttcacattct ctttttctta catcttatct ggtgcatgaa gtcctagcaa
     6661 acgaaggtct tctttcaata atacagtcac tgcataaact agagctaagc gtgcttcttt
     6721 ttgttcatca tcagctaaaa tcttcgtatg agcatagtat ttgttgaatg cttgtgctaa
     6781 tttgattgca tgtttcgcaa tcactgacgg ttcgtatttt tccccagcag acaagaccgt
     6841 ttcagggtat ttttgaacta atttaaccac ttcccagctg gtatcgtcat tcaatgcata
     6901 gtctgctgtt tctgaaggga caaatcctgc tttttccaac aggctgactg cacgagcatg
     6961 tgtatattgc acataaggtc cagtttctcc ttcaaatcga acaacttctt ccaggttaaa
     7021 gtcaaatgta ttcagtcgat cgtttttcaa atcgtggaaa atgactgccc caaccccaac
     7081 ttgtttagct acagcatctt tgttttccaa atcagggttt ttctcgctga tttgttcttt
     7141 agcagattgg attgcttcat ttaatacttc ttctaataag acgatctttc ctttacgagt
     7201 tgataatttt ttaccacctt gcgtgatcaa accaaatgga atatggtgca tgtcgtcaga
     7261 ccagtcaaag cccatttcct tcaagacagc tttcaactgt ttaaaatgat agctttgttc
     7321 attccctact acatatagag attgtttgaa gtcgtaagta cgtttacggt acaatgcagc
     7381 agctaagtca cgtgtaatgt atagtgtcgc accgtctgac ttcttaatca acgctggatt
     7441 caggtcataa gcagataggt caacgatctc tgcaccttta tcctcgttca gcaaatgttt
     7501 ttcttctaaa agtttgacga tttcatccat cttgtcattg tagaaagctt caccgttcaa
     7561 cgaatcgaaa cgtacttcca gcagatcata gattttattg aattctttca tcgattcatc
     7621 acggaaccat tgccataatt gaatagcctc tttatcgcct tcttccagac gtttgaacca
     7681 agcacgagct tcttcattta attctggttc tgtttcagct acttcatgga attggacgta
     7741 taaacgaagc aattcattga ttggttctgc tttaacagct tcttctgtac cccatttttt
     7801 gtaagcgacg atcagttttc caaactgtgt tccccagtca cctaaatggt tgatgcggat
     7861 tggctgataa ccgatttttt ccataatgaa accgatcgaa ttaccgataa ctgtcgaacg
     7921 caaatgtccc attgagattg gtttggcaat attaggagaa gacatgtcga ttggtactgt
     7981 tccttgattc ccgatgttgc tatccccata atgttctttt tctttcacta ctgtttgcaa
     8041 aacttttttg ctgataagtt ctttattcat gaaaaagttc aagtaaggtc cgacaacttc
     8101 gattttttca aagttggcag aatcgatttt ttctgctaaa tctgcagcga tttgctgtgg
     8161 cgcttttcgg taaacttttg ctaatgaaaa ggctggaaat gcaacatctc cgtgttcagc
     8221 tgattttgga ttttctagta attgctcgac ttgttccagt gttaaatcat ctttgacagc
     8281 gtcatagaca gcttttgcga caagatcttt attattcatc tttattcctc ctaaagtgtt
     8341 cccttgattg ttaaacaaaa aaaatctcta gcgacggcat acttagtatg ccatcactag
     8401 agacgagatt tcccgcggta ccactccaat tgtcctaaaa aaggaccagc tccatctgat
     8461 atcggtagac gaccgtctgt ttaaaacaga attctccaag tgcgcttcgt tgtatcttct
     8521 tcattcggct tccaccactt ccgaattcgc tagccaagaa tgatacaagt actctcttgt
     8581 tcaacaaatc ttagtcttat taaactttct tgaattatat cagaaaaaaa aacgaaaggc
     8641 tagtcgtttc ataaaaaaaa aatccagatg gcctaaagga gaagaaatac cacctggata
     8701 gctataaatc atttcgtaaa tacgaaagac ttaacaaatt aatcagctac gataatcgtt
     8761 ccttcttcag aagctaaaag attctcgata ttttctaaag aagtgatgat tgccttagcg
     8821 tttggttttg cttcaacaaa tgcaatagct gcttctactt tcggaagcat gcttcctgga
     8881 gcgaattgct tttcatcaat gtattgtttc atttctgaaa cagtaacagt ttctaatttc
     8941 ttttgatctg gtttttggta attgacatac acattgtcta ctcctgtcaa tacgatcagc
     9001 aaatccgcat cgatgatttc ggctaatttt tctgaagcaa aatctttatc gattacggct
     9061 tctcgtcctt ctaatccagt agctgtttct acaacaggaa tcccaccgcc accaacagag
     9121 actgtgataa ctccttgatc gaccagcgtt tcgatgacac gtgcttcttt aatcgagatg
     9181 ggtttagggg aagcgactac ttttctccat ccacgtccag catcttcttt gaatgtcaca
     9241 gtggagtcag cattcatttg ttctttggct tcttcttccg tgtagaatgg accgactggt
     9301 ttacttggat ttttgaatga aggatcattt tcatccacaa taacttgagt gactaaagaa
     9361 accacatctt tatcgatccc tttttccttc agtacttcgc ccatggcgtt ttgtaaccag
     9421 taaccgatag atccttctgt cattgcaaca catgtatcta aaggcattgc cggtgttttc
     9481 tcagaatcag cagcttgctg ctgaatcaat aggttaccga cttgcggacc atttccgtgg
     9541 gagatgatca attcatctcc ttgttcgata aatttcacta aatactttgc tgtttccatt
     9601 aacgcttctt gttgggcttt tgcacttgca tccgtagaca agatggcatt gccccctaaa
     9661 gcgacaacta cttttcgttt ggccatttca ttcgtccttt cttcagttct accgaatttt
     9721 ctagataaca aaacgagcca gcgttttgtg attttccctc aatgctgact cgttttttcg
     9781 tgagatgaat gtggttatgc ttaaacgcga gggataaaca agttacctaa tgttgctgcc
     9841 atgatcgctt tgattgagtg catacgattt tctgcttggt caaattgacg agcatatttg
     9901 ctgcggaaga cttcatctgt tacttccatt tcagtgatgc caaagcgttc tttcatttgt
     9961 tcaccgtaaa cagtgtttgt atcatggaaa gctggcaagc agtgcaagaa gatcaaacga
    10021 tctgtgttgt gtgttttttc taccatttcc atattgattt ggtaaggttt caacaatttg
    10081 atacgttctt cgaatttatc ttcttcaccc attgataccc atacatcaga ataaaggacg
    10141 tctgctccgt ctacaccttt atcaacatcg tcggtaatca tcaattgtgc accggatttt
    10201 tcagcaaagc cttctgccat tttgacgatt tcttcttcag gttgtaattc ttttggtgca
    10261 acgatacgca tattcgctcc taaaattgca cctgtgacta ataaagagtt agccatattg
    10321 tttcgaccat ctccgcagta agcaacagtg ataccttcta ctgttccaaa gttttcttgg
    10381 atagttaaaa agtcagcgat catttgtgtt ggatgccatt catcagtcaa gccattccaa
    10441 actggaacac cagagaattc tgctagttcc tctaccattt tttgactgaa tccacggaat
    10501 tcgattccgt caaacatacg tcctagtact ttagctgtat cttctgtgga ttctttttta
    10561 cctaattgga tatcatttgc acctagatat tcaggatgtg cacctagatc gatcgctgca
    10621 gtagtaaatg cagaacgagt acgtgtagat gttttttcaa ataaaagcgc aatatttttt
    10681 ccttctagat agtgatgtgg gataccacgt tttttcaagt ctttcaagtg ttctgaaaaa
    10741 tcgatcaaat attgtaattc ttcttttgta aagtcttttt ctgctaataa gcttcttcct
    10801 tggaatactg attctttcat attaaacatt ctcctttaga ttgtttttat ttaagattaa
    10861 agaggaaatc ccgacggcag ctgacagtaa gactgccgtc aggaaacaaa ttacttcaag
    10921 tcttcacgga caagtggttg gctcatgcag cgtggaccac cacgacctcg tgacaattca
    10981 cttgaattga tttctaacac tttgatgcca tagctacgaa gcaattcgtt cgatacataa
    11041 tttcggttat aagtgactac aacacctgga gcgattgcca atgtgtttga tccatcattc
    11101 cattgttctc gaggtgcaac gatctcatct ccattaccag ttgggatcag tacaagatca
    11161 tctaatccaa gagcagcttt catcgtttcg tgtaagttat ctgaatgagt aatcttgata
    11221 tcatcaccat ctggttcgat tgtaaagaca tccatcttac cgtctttact ttgaattgct
    11281 ggatggatag tgaatttatc gtgatctacc attgtaaata cggtatccaa atgcatcatt
    11341 gcacggttgt tcggaatttt gattgccaac actttttcaa atcctgaatt ttttgcgaat
    11401 aggtggcgtg ctaatttttc taaagctttc gcattagttc gttgagaaat cccaacagct
    11461 actactttat cgcttaagat caattcgtct ccgccttcga tatgatctgg attttcacga
    11521 tccaaccata cttcgattcc tttatttgca aatcttggat gatgtttcaa aatatattct
    11581 gtaaacatcg attcacgacg acgtgcttca aaagtcatct tattgactgt catcccattt
    11641 cccattgatg cggaagggtc gcgtgtaaag tacaagtttg gcattgggtc catgtaaaat
    11701 ggatagtcat cgtctgctga cagatcatat agactgctag aacgtacttc gatatctttt
    11761 gtacgaaccc cagccatgat tttatctacc atttcttgcg tttccattga tagaaggaac
    11821 tcatgaagac cctctcttac agcattcgat gcaatatgag attcatccaa cattttattc
    11881 aagaattgtt ctttgacatt gcctgcgtca atcgcttctg ccgctaattt ttccaagtaa
    11941 agagtctcga cgccttcatt ttgcaatgtc ttcgcaaagt tgtcatgttc ttcttgagca
    12001 atcggaagat atggaatatc atcgaacaac agacgatcca taatatctgg tgtcaaattt
    12061 tccacttctt gtccaggccg tttcagtaaa actgtcttta atttccctat ttcagagaaa
    12121 acgtgaatag gtttatccat gttgtgttgc ctccttcttt ttgattacat gtataggata
    12181 tcctcctttt gtaatcactt tcataatttt gtctcattca aaatgagaca ttcctcacat
    12241 taatattcga taattaaata attaaacgtt tttctatttt taagcgtaat ttattcattt
    12301 tttat
//
LOCUS       DACDUU010000082        11967 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.082, whole genome shotgun sequence.
ACCESSION   DACDUU010000082 DACDUU010000000
VERSION     DACDUU010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.082"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            98..877
                     /locus_tag="IWL30_002404"
     CDS             98..877
                     /locus_tag="IWL30_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAP9707129.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            870..1187
                     /locus_tag="IWL30_002405"
     CDS             870..1187
                     /locus_tag="IWL30_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP9707130.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            1431..3041
                     /locus_tag="IWL30_002406"
     CDS             1431..3041
                     /locus_tag="IWL30_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9707131.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            3198..4046
                     /locus_tag="IWL30_002407"
     CDS             3198..4046
                     /locus_tag="IWL30_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9707132.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            4277..4756
                     /locus_tag="IWL30_002408"
     CDS             4277..4756
                     /locus_tag="IWL30_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707133.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            4789..7017
                     /locus_tag="IWL30_002409"
     CDS             4789..7017
                     /locus_tag="IWL30_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP9707134.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            7146..8789
                     /locus_tag="IWL30_002410"
     CDS             7146..8789
                     /locus_tag="IWL30_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP9707135.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(8862..9407)
                     /locus_tag="IWL30_002411"
     CDS             complement(8862..9407)
                     /locus_tag="IWL30_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAP9707136.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(9430..9915)
                     /locus_tag="IWL30_002412"
     CDS             complement(9430..9915)
                     /locus_tag="IWL30_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9707137.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(9905..10717)
                     /locus_tag="IWL30_002413"
     CDS             complement(9905..10717)
                     /locus_tag="IWL30_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAP9707138.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(10856..>11967)
                     /locus_tag="IWL30_002414"
     CDS             complement(10856..>11967)
                     /locus_tag="IWL30_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP9707139.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT         3861 a         2215 c         2390 g         3501 t
ORIGIN      
        1 aatataatct tgtttttaaa taaaacggct acaatttagc gaatttatgt tacaatgagt
       61 ccgctgttta tttgttgtaa ttaaaggaga tattttaatg aacaaaaaga aaagtgcgcc
      121 atggcttcaa gctttgcatg cagtgatgcc actttgtata agctatatac cagtaggatt
      181 agcttgcgga gtattgttgc aaaaagttgg atttgatcct ctcttatcag gactgttatc
      241 gattctggtc ttttccggtg gcgcgcagtt tttagttgca tctatgctta cgacgcaagc
      301 atcatttgca acgacattat tgatggtttt ctttttagaa ttacgctata cattattagg
      361 agcaagtctt gccggtttta tgaaaaaaga aaagcggacg tttttggctg tcttttctca
      421 atcactgaat gatgaaaact atgcggtgaa ctacttaaag ttttcaactg acccttcctg
      481 gaataaaaga aaggctttat atgtcaattg gttctcaatg actgcatggg ccggtagcaa
      541 tatgatcggt aattttttcg gctcggtgat tcgagtagat gctgaccttg tccattttgc
      601 tttgacggcc atgttcattt tcatgtttat catgcagatg aaaagcgcac tactgatctt
      661 tacaggttta ttctcaggcg tattaggggt cgtattcatg gtattattcc aaaatacttt
      721 cggattgatt gcagcaacag tcattgcttc atttgccgga tttatgctgg agaaggcatt
      781 caaaaaagaa aaagctaaga aactaagaga ccgcggtaaa gatgtcaatg agccgctctt
      841 caacttcccg gaacaggagg ccaatcacca tgactaaact atatttgcta cttttagttg
      901 cttgtctgtt catcgtggca tatatcccgc gcttgttccc gatgctttat tttactcatc
      961 gaaaagtgcc atcctggttc agtgactgga tgaaatatgt accggtggca ttatttgcgg
     1021 cgttagcttt taaagatgtt ttcattactc atgaacatct ggatattgct tggaatatca
     1081 aaatagctgc aatgattcta gtagcaggcg tggcttacaa aacacgttcg atggcgttgt
     1141 cagttttgac aggacttgcg tctgtctttt tgctgtctat gctttaaact gttttatttt
     1201 attaaaaaca gagattgcga cataagttgt tcaaactgat ctatccgaac tgcatttgca
     1261 acagtcaatt ctggatgcaa gtttattcgt agggataaaa aagattagtg ttggcttatg
     1321 atgcaagctc ttttttattt taggaattag agagggattt gtccttctat gattttttgc
     1381 tataataggg aagatgttta ttttatgaaa gaatagttga gggaatttaa atgaataaaa
     1441 aattgatgca aggaacattt tggctaacct ttgctaatct tctatgtaaa gttttagggg
     1501 tagtctattt gattccctgg ctaagtatga tggggaacaa tcaagatggt atgcttgcta
     1561 cgacattgta taatgttggc tatcttcctt atggattgtt cctgatgctt ggtacagtcg
     1621 gatttccaaa tgcgattgca aaaaaagtag ctgtagccac aaaagaagga gataacgctg
     1681 cttgtcggct gatttttaga agtacgatca atatcatgtt tgtcatcggt atcgcctctg
     1741 ctgcactgat gtatttattt gctcctttac tagccggaat cagtccgatt gctaacgtta
     1801 ataatgggat actagctatc agaagtttat gcccatcttt gattgctatc ccaattctta
     1861 gtgcaatgag aggatatttc caaggaaaaa atgatctgcg gccttatgga acttctttga
     1921 ttatcgaaca ggctgtgcga gtgatcgtta tcttggctgg tacttattac ttgcgcgtat
     1981 tgactgacgg aaccatatta gaagctgtat tgatcagtac agtcgcttct ttcttcggtg
     2041 ggttagctgc gattatccat atgtatatcg tcggacgcag aaaagacttc ttcgaactta
     2101 aagatttttt gatttccagc cgttatttcg aaagagaaaa ccgatctgcc tctgtctcta
     2161 tcattcggga aacactaccg tttatttttg tcggctcagt cattactatc gttcaaatga
     2221 tcgaccaagt aacgatgaaa ccactgcttc actttttccg tccagagatc gcagatcagc
     2281 aactggaatt cttattcagc cgtgcttctg tcaatccaaa taaattgaca ttgatcctga
     2341 tctccatggt aggaacagta gctataagca gcttgccaat attaagcacg atgaagaaaa
     2401 aagatcggat acaaatcgaa aaaacagtag gagatagttt ttcgattgcg ttattgattc
     2461 ttcttccttc attgactgga atgtctttat tagctggacc actatacaca ttgttttttg
     2521 gctatgatcc tgaaagtgtc ggatatttcc aaatggcttt gctagcttct ttgtttttct
     2581 ctttattcac gatcctatct acgatgcttc aatctttgaa tcaccatcgt tttgcgatcc
     2641 gattaactgt agaagcaatt atcctgaaag tcatcttcca agtaatcgga ttaggattag
     2701 ttggcggata cgggatgagc ttatcaaatg gcttagcatt cggtattgtc tttgttcgcg
     2761 gctatcactt tatgtgcaaa gaataccgaa tagcaccttt agcaaagatc agcaattttt
     2821 tcttaaaaac tttccgctcc accctgatta tgcttgcagt atgttttgcc gttttctttt
     2881 tgctgaatca gcgattgtca atggaatcaa aaagctatgc aatgatttat tgtgtggctg
     2941 taggaagtat tggcggctta gtatttttat tttctcaatt cggaaaaaat gggtttcgaa
     3001 tgttgaaaaa tgttaaacat catagcagaa gcaaagaata ggttaactaa tcgttttttg
     3061 ctgtcacttg aaaaacggca actcgtatga ttacgcttgt cgtttttcta ttttccaatt
     3121 ttagtttcat atctaatctt acttatgata tagttataag gtctggaaaa attctttgaa
     3181 acgaggaaat taaacacatg aaacttgcag tagttacaga tagttcggca tatctgccgg
     3241 aacgtatcaa gaaccatccc gacttattcg ttattccaat ccctttgatt atggatggga
     3301 aaatttataa cgaaggaatc gatatcgaag cggatgaata ttatggactt ttaaaaaaca
     3361 gtaaagattt tcctactact tcacaacctg tcgtagggga agttttgggt ttatatgagg
     3421 aattgaaaga gaaagggtat gacacgattt taagtatcca tctttcttca gggatttctg
     3481 gctttgtcaa cacattattt gcgatgaaag atgacatcag cggagtaacg gtcattcctt
     3541 atgactctaa gatcactagt atgccgatgg gacacatggt agaagctgcc ttagacttaa
     3601 atgatgaagg tcagtccctt gatgaaatac ttgcacatat cgatcgaatc cgagataata
     3661 cctacgctta tctgattgta gatgatttga acaatttagt acgaggagga cgtttgacaa
     3721 atggtgcagc gctgattggc agcttattaa aaatcaaacc gatcctgaca ttttctgaag
     3781 ggaaaattgt tttattcgaa aaaatccgtt caagtaaaaa agcatttgct cgagctgaag
     3841 aaattattgg tagaagaaat aacgaaatac aaccgccagt caaactttac gtgatacatg
     3901 caaataattt agaagtagcg attgcggaga aagagaaatt gcagaaaaaa tatccaaatg
     3961 cagaaatcga aattggtcat ttcggaccag tcattggtac acatttaggt gaaaaagcca
     4021 tcggcattgc catttcagcg caataattcc ttttattcaa aaaacatacc aaaaatattc
     4081 caacatcatc ttggactttc tacatgttac taatgagcgt gtaaaagaac gagatggtgt
     4141 ttttatttct atcaatgaag ctctttttct ttgcaatcct cacttcaatc ctctataatg
     4201 tcgcatatag tttagtaaat gagtaaacta ctaatttact tatttactaa aaataaaagg
     4261 atgaggagtt aaaataatgg aaataccaac aaatttaaaa cataaaccag taatcgttgt
     4321 agaagactat gataaagttg atggaagaaa tgcattaaat acggatgcaa aagggctttc
     4381 actgggcctt gctcagtgga atgatcgtgg aaaagtcgat atctcaggca aggtatggcg
     4441 acatactgga gaaaaatggt cacgacagtc ggaagaatta ccaatcacac gtattttaga
     4501 tttagcgatc ttagctgcac aaggaagttt atacttccaa gatgcttatc gttatgaaaa
     4561 attttatgat ccagaaaatc cagtcgtaga catcattgga ttacaaggga accgaatgac
     4621 ggtagaagta gataccaaaa atccaaagat cgatgaagac atcattttgt attacgatac
     4681 actacaaaaa gacggagaac tgattgggga acgattccgt attttaaaac gtcttttaga
     4741 tgagctagga tattaataga cagagatgat caaaagggga agagaagcat ggagaatttc
     4801 tcactaacag aaaaccgaac cttttcacgg ctagaaaagc aaatgatctg gaagaaaccg
     4861 aaaagccatc ggacaagtgc agaagaatgg cggatcgcct ccgaaatcaa agggaattgg
     4921 caagatcctg aaatgaaaat cagtaacgta cttttggaag gagatgccgg gtcggggaag
     4981 acacaattag ccaaagcact atctgctgat ctgcaacttc cctatacaaa agtgacctgt
     5041 tttgcagata tggacaaatc agatgttttc ggagccttat tacctgtagt agattctaat
     5101 aaggaacaag accaagaatt actagaagct atttaccaaa cagatacact tgaagctgta
     5161 ctgacactga tccagaacca ttacggcatt gatccttttt ctgctaaaga aaaattagca
     5221 gagctagttc aaaggatcga agctgattat ccagatgcaa tccattacag ctattaccct
     5281 tctgaaatcg ttcgagcgat tgaaaaagga tatttattag agattcaaga gccgactgtc
     5341 atccgagatg cctctgttct tgtggcactt aactcagcgc tcgaaccaaa tgggatgttg
     5401 aatttaccga caggaatcat tcgtcgccat ccagattgcg tcattgtgat cactacaaat
     5461 cgaaattacc aaggaaatcg cccattaaat gaatctttaa gagatcggat gcaacatgcg
     5521 gaaaaaatgg atcttcctgc cttagaagtc atggcagaac gtgcgatggc gaaaactatg
     5581 atccaaaaac aagaacttct cttaaagatg gcagagatta tccgtttgtt ggatgaaact
     5641 gcaaaagcta acgccatcaa aggtgtagca ggaatgcgtt cttattttta ttgggcaaat
     5701 acctataagc aaggacaaga tttactccag tcgatttatc caaaagtttt gtataaatta
     5761 tccactgatt cagatgaatt ggcaattctt gaacaagctt tgtctgacag tggattgcta
     5821 ctggaacttc aggacatatt gagaaaagaa aaatggggtc atcctcaatc tgagcataca
     5881 aaaggaagaa ccattagcgc cgaagaagca aatgaacgaa acatcactga aaatgctgaa
     5941 gaaattctgc gcttagatga aaagaaatcg ttgtctgctg aagaaccaaa tgagggagaa
     6001 gagaaacaag aagaaacaat agaaaaaaac gagattcctc ctcaaatgca aaaagaacgc
     6061 agtagcgata tcgaaggaaa aaatcaaaaa gaacaacaaa caggagatga ttctcaagaa
     6121 atgtctgctt ctgacatgga agcacatgat aaggaaatca agaagcaact gaataaagaa
     6181 gcgcgacaaa tcatgaagga aacgattcat gaaaaagaag gtctcatcgt tcaccgacca
     6241 gctttccaag gaaaaacgcc ggaagcacgt gagctacaca tgcaagtaat gccaatagta
     6301 gaatcactta gccggcaaat tttagagtta ctggataatg aacaaacaga gacgtaccaa
     6361 aaaggaaaat acgaaggtca gcggttcaat gcaagcaaag tagcatatgg tgatttgaga
     6421 aattttgaca aaaagaaccc gccacatgag caaccttctt tagcggtagc tgtacggatc
     6481 gatgaatctg ggtcaatgat acgggatgat cggatcgaag cggcaaaaaa agcagcgatt
     6541 gccattgccg aattttctaa gaaagtcgat atcccattgt tgatttacgg agatacagca
     6601 gaccgttcgt caagagaaaa gacttctttg ttttcttata aagaatttga agacggattt
     6661 caatggttaa atgaaaaatt agtcactatg aaaccaaggc aaaacaatcg tgatggtgct
     6721 gcacttcgtt tagcagcaga aaaattaaac agacaatccg caacaaccaa actgttgctt
     6781 aatattagtg acggtcagcc aaaagcgttg ccggattata caggcaaaaa agcaaaagaa
     6841 gatatccagt ctgttttgaa agaatatgaa cgtcaaggaa tcctatttgt ctccgcagct
     6901 attggccaag acaaagaaga aatcaagtcg atctacggtg aaagtcgttt tgtagatatt
     6961 actgacttga atgaatttcc caagcaaatg attcaactga ttgcccgcta tctatgataa
     7021 aataaagctc aacgaacgca cttagcagat tcgttgagct tttcgtttct agttaccggt
     7081 aacaaggata cttctaaaat agaatcggtt ataatcaaga gggaccgtta aaggagtgaa
     7141 acgatatgat acaagaacaa aaatggtgga aagaagcagt aggctatcaa atctatcctg
     7201 ctagtttcaa agatagcaat ggcgatggtc aaggagacat caatgggatc cgtgaaaaac
     7261 ttaattattt gaaagatctt ggtatcggct ttatctggat caatccgatc tatcaatctc
     7321 catttgtcga taatggttat gacgtaagtg actatcaaga tatagaagag agattcggaa
     7381 cgatggaaga ttttgattta ttgctaaaag aagcacatga attagggatc aaagtgatta
     7441 tggatcttgt catcaaccat agttcggatc agcatcaatg gtttgaagaa tcgaaaaaat
     7501 ctaaagaaag cccgtatcgc gattattaca tttgggtcga cggggtagat ggcaaagaac
     7561 cgaataattg gacatcgatt ttcggcggtt ctgcttggga gtattctcat gaaactggac
     7621 agtattatct gcatgttttt gcaaaagaac aaccagactt aaattgggaa agcgagaaat
     7681 tgaaagaaga actattcaat atgatccgat ggtggttgga taaagggata gatggatttc
     7741 gtttagatgc gatttctcat gtaaaaaaag atgaatattc agtaaaagcc acagagaacc
     7801 ctttctcacc ctttcaaaat gtctcaggaa tcgaagaaca tttgaccgat ttgaaacatg
     7861 tttttgaaga gtacgatatc atgactgtcg gagaagcaag tggtgtcact gcagaagaag
     7921 gaccacaatg ggtagggaaa gacggttatt tcgatatgat attcgaattt gatcatatcc
     7981 atatctggca gcaagaaaaa gaagggcaat tagatgtcct taaattgaaa cttgcattaa
     8041 gcgcttggca aacttcattg gatggtatcg gatggaacgc tttatatatg gaaaaccatg
     8101 atgttcctcg tgccgtttca gtgtttggag atactcgtcc cgatttttgg gctatgtctg
     8161 caaaagcaat cgctatgatg tatttctttt tgcaaggtac gccatttatt tatcaaggac
     8221 aggaaatcgg aatgacgaat atgccctttg aatcgatcga tcaagttgat gcagtagatt
     8281 cgaaaagact ttataaaagg ctgcttgcgg aaggtaagac aagagaagaa gcattggata
     8341 tcattcgtga aacaacgaga gacaacagca ggacacccat gcaatggact tcggaacaat
     8401 atgctggttt ttctacacat gaaccttggc tgatcactaa tccaaatacc aaaacgatca
     8461 atgtagaaca acaagaatat gaaccagagt cggtcctgca attctataag aacatgatcc
     8521 gcattagaca aaccaataaa ggtctgatat acggttctta taaagaatat cttcatgaac
     8581 atccacaact ttatgtctat gaaagatatt tagaagatga acaatatctt attatggtaa
     8641 acttaacaga atcacttgct gactatgaac ttccaaaaga agcagatcag tcctggacat
     8701 tactcttgtc aaattcctct agcggagaat ttgaagctaa aggcatacta gcaccttatg
     8761 aagctcggtt atataaaaca aacaagtaaa acttcctcgt taaagttcat tagttggact
     8821 ttgcgaggaa gtttttcaga ttgctctgtc aattactcat ttcaataatt cgctctagga
     8881 ccaccgatat atttaggacg agagcgaagg gctgtcacgt gagcttgtcc aagggttttc
     8941 aaacgaggtc tgacaggtac ttggacatgt ttgatatgca taccgattga tgtgtcaccg
     9001 atatcgatac cggcttgtcc aactacgtgt tccacttcga caggatcatt gaactggtca
     9061 aaagcagcaa cagaacaagc acctccagca tgtagtgcag gaacaacaga gacaatttcg
     9121 aaattttttg cttctgccac tgttcgttct actactaatg ctcggttcaa atgttcacaa
     9181 ccttggacag ctagcgcaat ttctttagga tccaacaact cttttaacgt acggatgatc
     9241 cattgaccaa cttccgcact ggaatttttt ccaatatgtc ctccaacgac ttcgcttgtg
     9301 ctgcatccta aaacaaaaag atcacctttt ttcaagtgtg cctcagccaa tatgtcatta
     9361 actatttcag tcaattggtc ttttaattct ttttctgaca acttcatttt ctaaaacctt
     9421 ctttttcaat taatattgct tccgtgcttg aatacgttga agacttgcag ttataggaaa
     9481 ggcaagtccc attccaatca gattttggaa gatgtttcct ggtatagaag ccactccagc
     9541 cggccaatca tataaaatcc aaccggcgat accatagccg atgatcataa ctaagcttcc
     9601 taataaaagc cctaatcccc acatacgagt agaagatttg ctgaaagaac cagcaacata
     9661 tccttgtaac ccgtggatca ctaatgaaaa aactgcccac tgcggatatc ctgaaatcaa
     9721 atcgatcaat agaccactgg aagcaccaac aactaatcca cccgttggac cataaagaaa
     9781 agcagcggta tagatcccgg cctcgcataa tgtcacaaat ccattcgtag ccggtacagg
     9841 gatcaaaatt gttaatgaaa gggcaactgt caacgcaatc agcatagcat agatcgttaa
     9901 ttgcttagtt ttcatttctc attcctcctt tttgaatacc gccagaccag acacttccgg
     9961 tatgatcgtt catcagcacg ccattagaaa tacaatgggc aacaaatttt ttactttctg
    10021 ctactgcttt ggtaaaatct gcccctaaca caagattcgc tgttattgca gaagaaaagg
    10081 cacaaccggc cccgtttact gttgtagtgt cgattttttt atgagaaaat acctcataat
    10141 gatcaattga atagagaaga tccacagctt catctccagc catccttgtg ccgcctttga
    10201 tcacaatgtt ttttgttcct gaagcagtga gttctttagc gagatggatc atttctgtta
    10261 acgaagaggg aagagcttga ccagaaagta aagcagcttc tgccaaatta ggcgtaatca
    10321 tcgtaacaag tggaaaaagt tcttcgatga gctgcgaaat atacaattga ttgatcacag
    10381 cttcagtttc tttgaatgcc aaaacagggt ctaaaatgat tgggatattt tgttttgttt
    10441 ttatgaagtc tcgaacaacc atgattgctt cacttgaatt caataaccca attttgatac
    10501 catctaatga agtcatttta tcaatcgaaa ctaactgtcg tttaagcaat tccaaagata
    10561 tagattccag cataaatcgc tcattaatat ctagtgcccc tatagaagtc aaggcgctta
    10621 gtccgaatag accaaaattc tcaaaagttt ttaaatctgt ttggattcct ccaccagcaa
    10681 atggatcaga accgccaatt gtcaagattc ttttcataac attttcctcc atgaataaga
    10741 gtataacgaa attttcaaaa aagaaaaaca gccaattggc atatttttat ctcatccaat
    10801 ttaaaaaaac aaaaagaaaa gaaagaccaa aatggatctt tctttcctta aatagttagg
    10861 acttttgtga ttttaattct ttctcgattt gaagccgttg cactttcatt gtagctgttc
    10921 gcgggattgc atcaaaatca cggatgatag ggacgttcaa gcgaggaagt tcatggactt
    10981 gctcccacca tcgattccaa tccatttctt tatcaggagc taaagcaata atcggttgtg
    11041 gagaatgatt tttatccctt acaatcacta cttctgccaa aaaatctaac gaatctaata
    11101 aaaagtcttc aatagctaat gtactattga tcgtttcgat caaatccact tgccgatctt
    11161 tcaaaaatag gtggccatgc ttatccataa aaccgtaatc tccgctatcc caccaatcgc
    11221 cataaacatt ttcctggaac cgtgcatctt ccttgtaata agtcaaagcc cgtcctttag
    11281 acagtaattg tatatgacca tctgtgtttt caggtaggac attcccaact gaatctgtaa
    11341 tccgagcgct tgtcatgtct tccagtccaa cgcccatatc tcttgcatca cttgttttca
    11401 aactctctag tgtatgagcc ttaagaatca ttggtccgca ttcactttgg ccatatactt
    11461 gaagaaatac cgcatcctgt gccatcgacg ttcgcaaaaa tgtcttcatt gtctggttgt
    11521 ttatagcatc aaaagtggag tgatagtatc ggatacccga aaaagcttct ggatgctcct
    11581 tagcagtaaa acgccactga acaaagttat tcggatgggt ctctagtgca attggccggt
    11641 gagcttccaa catatgaaca acttttgaac tttgtgcgtt tgctaaaggc atcataggaa
    11701 atcccatgga catcaaagaa gagataccaa tattgaatct ggaatgcact ggagagatgt
    11761 gaaaagcgat taatttcttt tccgaaattt ttgtgaaaac tgttttttgc catttcgtcc
    11821 gccatcccat agaatgagca gaatgacaaa taagtttagg gataccagtt gtaccagaag
    11881 tatgggtcat ataagaaatt tcatttttgt ctaaaaatga ttgagaaaca gacaaagcag
    11941 gttgttccat taaataaaga ataggaa
//
LOCUS       DACDUU010000083        11647 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.083, whole genome shotgun sequence.
ACCESSION   DACDUU010000083 DACDUU010000000
VERSION     DACDUU010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.083"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            560..1531
                     /locus_tag="IWL30_002415"
     CDS             560..1531
                     /locus_tag="IWL30_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAP9707140.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            complement(1609..1845)
                     /locus_tag="IWL30_002416"
     CDS             complement(1609..1845)
                     /locus_tag="IWL30_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9707141.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(1814..1945)
                     /locus_tag="IWL30_002417"
     CDS             complement(1814..1945)
                     /locus_tag="IWL30_002417"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9707142.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            complement(2021..2404)
                     /locus_tag="IWL30_002418"
     CDS             complement(2021..2404)
                     /locus_tag="IWL30_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP9707143.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            complement(2437..3222)
                     /locus_tag="IWL30_002419"
     CDS             complement(2437..3222)
                     /locus_tag="IWL30_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAP9707144.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            3400..4500
                     /locus_tag="IWL30_002420"
     CDS             3400..4500
                     /locus_tag="IWL30_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9707145.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            4490..5314
                     /locus_tag="IWL30_002421"
     CDS             4490..5314
                     /locus_tag="IWL30_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAP9707146.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            5320..6126
                     /locus_tag="IWL30_002422"
     CDS             5320..6126
                     /locus_tag="IWL30_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9707147.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            6123..6953
                     /locus_tag="IWL30_002423"
     CDS             6123..6953
                     /locus_tag="IWL30_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9707148.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            6960..7991
                     /locus_tag="IWL30_002424"
     CDS             6960..7991
                     /locus_tag="IWL30_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9707149.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(8098..9371)
                     /locus_tag="IWL30_002425"
                     /pseudo
     CDS             complement(8098..9371)
                     /locus_tag="IWL30_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            9648..9857
                     /locus_tag="IWL30_002426"
     CDS             9648..9857
                     /locus_tag="IWL30_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707150.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            9907..10623
                     /locus_tag="IWL30_002427"
     CDS             9907..10623
                     /locus_tag="IWL30_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAP9707151.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(10525..11045)
                     /locus_tag="IWL30_002428"
                     /pseudo
     CDS             complement(10525..11045)
                     /locus_tag="IWL30_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            11333..>11647
                     /locus_tag="IWL30_002429"
     CDS             11333..>11647
                     /locus_tag="IWL30_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9707152.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPV"
BASE COUNT         3308 a         2181 c         2670 g         3488 t
ORIGIN      
        1 ataacgtgat tttttgctgt aatgacaagg ttttagctgt ttcagatacc atgtatccaa
       61 aaatagttta ttgagaaaat aggagagtat ttgatactaa ctagtaggaa aaaatgagat
      121 aaaaagtaaa tggaatcatt taaggtaatc tgaaataaaa aatatacagc cctgtggtac
      181 tttacctaca aggctgtata tattttttac tctcagcgtt tttgactccg tgtctgactc
      241 cgttttattg attttgaatg ttattcaatg accatttaac aggtcaaatt tgatgttttt
      301 gattccgtat gacgccgttt gatatctagt gatatagtga tttgagtcta catcgaataa
      361 gggaataaac caataaaaaa tgatttagaa tattaaaata acaaaaaaga ggactacctg
      421 tattagtcct cttttttgac cagattttga ccagtctatt ttacatccta gtatatttat
      481 atgagctaat tttatgatta aatggtaagt ttttccttgt gtgttttagt ttaaactagt
      541 gtgtaattca aaaatctata tggaaggaac agtattaaga atagaacagg gttctctaca
      601 tgatggtgct ggtttacgga ccgttgttta tttaaagggc tgcccattac gctgtgcctg
      661 gtgttcgatt cccgaatcac aaagcaagca gattgaaaaa ggatttggcc agaccatgac
      721 cgctgaagag gtaatggatg agattgagaa ggatgctgtt ttttactttc actcggatgg
      781 cggagtgacc attagcggag gcgaggcact ggttcaggcg gattttgcta aagaaatttt
      841 gcaaaaatcg aaatacattg gaatcaatac cgtactggaa accagctttt gtggtgctta
      901 taatgaaatc caaaaggtag ctccctatgt ggatacattg tttgtggatg taaaaatgtt
      961 taccagcaag ctccacaaac aatggaccgg cttagataac caacaaattt tgaagaatat
     1021 tcgtcgcttt ttaattgagt atcctaactg cgaagtgcga attcgagttc ctgtggttcc
     1081 aggtatcaat atgaacctga ccgagctgct gacgattgct tgttttgtgg cagatcttga
     1141 tcggttcgta ccgttagaat tactaccgta ccactgctat ggtatgcacg gttaccaagc
     1201 attgggactg gagtatccgt tggcagacac gccagcgcct tcacctaaag aaatgtttgc
     1261 attggcagat caactggctc gcacggttcc tcatttgctg gtcacagctt taaccaaaac
     1321 atttatttat ttattgaatc caagctttat cttatggata aatgaaagac actcactagc
     1381 tgcagccagc tcaagaatag agcttttgct gaagtcagtg agtgtctttt tacgatgttt
     1441 tttcgaagtt aatttgtcga atgaacactg gctcaatcgg tacatcttca ctgtttgtcg
     1501 gctgagctgc gatcgctgct acgatttcta acccttggta tacctgtcca aaaacagtat
     1561 aacgaccatc cagctccggt agtcctccaa gctccaagta tgcttgcttt acattagcag
     1621 gtcagcctgc gcctttgtgt tttgcacaat aaaaaactgg ttgccgttgg tgtgggggcc
     1681 gtggtcccct aaggccaagg cgccattaaa atgataaagt ccgcgatgca cttcatcagc
     1741 gaaaaagccc tcatagctgg aaacagcttc atcaccagaa ccatccaaag cgccaccttg
     1801 aataacaaat tccttaatga cacgagccga aaggtgtgtt gtcataaacg ccttgctcag
     1861 ctagggagcg ccagttggct acagccaggg gagcttggtc aggaaataag cggagacgaa
     1921 tttcaccagc atcggtgacc agcacacaaa cttcttcctg ctgcggtttt tctaattgaa
     1981 agagaggaag gtttgcaact gaaacgcctt gatcctgcca tcaaatatcg aaaatccgcc
     2041 actcgacctt cgcctgatca ccaaggcgag ccattcccaa taaaacggta taggcggttt
     2101 catccgcttg aaaggtcaat tgcagctgtc ctcgtaaatc gttgtagtca tctggggcgg
     2161 ctacttgata aaaaagatga aactgcgcat gcaaatattc caaaggctga tctttaaagg
     2221 ctgcaaaata agcctgaact ttttcccgct gcaagctgtc agccaccaaa aaattttcca
     2281 gttgttgttt tggaacggct gctaacaagg atcggcaaac cgcctcaatt ttcaacgcat
     2341 cccaatgtcg cgcaattaaa taatggatgg gtgcctcttg attgtagtcc cacaggttgc
     2401 tcattttttt caccactttc ttctattata taggaatcac aaaatcggca gcggcgtcag
     2461 gcggcgttct tttccttgaa tgactagcaa cttttctacc tgacagtctg ccaacagctg
     2521 actcattttt ttaaagccca gctgataatc ttcggcaaca tgagcatccg agcctaaggt
     2581 aaagagcttt ccgccgacac tttgatacaa aggaacggca taacggtaaa ggtcttcgtt
     2641 gccgtatttc aagaagctct tggcatttaa ctccatcgcc aattctcgtt caacaacctt
     2701 tttgaaaatc gttagcagct gagcctcaaa atgctccgcg aattcttccg ctgtgaaggt
     2761 gaagcggcgt aatccataat caaagtgagt caaaatatct ccttctggaa aatccgtcaa
     2821 gacctgcaac atctgatcaa aatattcctt ggtagtttga atgggatctt tggttaaaac
     2881 cacatcgtcc atataatcaa agcggccatt ttgatgaata cttaccagct ttaaatcata
     2941 aggatgctga gcgaggtaat ccacgatccg ctcttcttgt cctggaacaa cgccaatctc
     3001 gatacctcgc agaaatttgg tggaataggt ttcttttaaa gagtccagtt tgtttatata
     3061 ggctggataa tccggaatgt catcttttcc ggtagatgga ttgtttaaat caaagtgatc
     3121 cgtagaaacg aaaaactccg gctgataggc taaatagttt tcaaattttt cctctgaatc
     3181 aaaggaaaaa tacgtgtgga gatgctgatc ataatactgc atagggagcc tccttttttc
     3241 tactatcata ccgcaaaagc tttagaaggt taacgggaat tcgtcgaaat ttacaaacca
     3301 tatacaacct ttacattgtt catgaaacct tttcaagaaa ctgattctct cattacaaag
     3361 ctttgttaga atggaattat cgaaagggga gaagaatgaa tgaaaatgaa tgaaaaaatt
     3421 cgtaacatga ctatcggttt attttgtgca gcaacagtaa gtatcctgct gacaggctgc
     3481 ggctcaggtg aagcaacgaa gcaatcggct gcggcagacg actctttaga tacgattata
     3541 gaaaaagcca aggaagaagg agagattgcc agcttgggaa tgccggatac ttgggcaaat
     3601 tgggtaggca cttggaacga ccttgaaaaa gaatacggct taagtcacgt ggatacggat
     3661 atgtccagtg cagaagaatt ggctaagttt gaatcagaag gaaaaaatgg aaccgccgat
     3721 attggtgatg tcggtatcag cttcggtccg ttggctgaaa gcaaagagtt aaccttacct
     3781 tataagacca actattggga ggaaattccg gattgggcaa aggatgatga tggggattgg
     3841 ttgttaagct atacgggaac catttccttt atcaccgata aagagaatgt gaagcaagca
     3901 ccgactagct ggaaggagct gcaagagggt gactatcaag ttagcgtggg ggatgtgacg
     3961 attgccaacc aagcacaatt tgctgtattg gctgcggcga ttgccaacgg aggtgacgaa
     4021 aaggatattc agccaggtct gaattactac aaaaaaatgg cagaaaaagg ccgtttgtcc
     4081 accgttgatg ccagcgtagc aaatcttgaa aaaggtgaaa tcgatgtggc agttgtttgg
     4141 gacttcaatg gactaaatta tcgtgaccaa attgactccg agcgttttga ggtaactgtg
     4201 ccaacagacg gctccatcat tagcgggtat acaaccttaa tcaataaaaa tgcccctcat
     4261 cccaatgcgg caaagctagc acgggaatat attttatctg atgaagggca aatcaatttg
     4321 gcaaagggct atgcgcgacc tattcgcaag gtagagctgc ctaaagaagt agctgaaaaa
     4381 ttactgccgg aggatgctta tgaagcggcg gtgccagttg aagacaatga agcttggaac
     4441 aagacctctg tagagcttcc agagctttgg caggcggagg tactgaccta tgcaaagtag
     4501 acttgacaaa ctgattgtag ttatactaga cggctgtcgc tacgacacag ccgtcgccca
     4561 gcttgggttc atgaatcatt tagttgagca ccagcaagca agcttattga aggtaaaaag
     4621 tgagctgccc agcaattcac gtccgttata cgaagtactg atgaccggcg ttccagccta
     4681 tgaaaatggc atctatagta attaccatgc tcagcgatcc aaagagtggt cgctgtttga
     4741 attagttaag gaagctggcg gaaaaacagg agccgccgct taccactggt acagcgagtt
     4801 gtacaatcaa gccccctata atcgtttggt tgatcgcatc cagcatcagg aggagcgact
     4861 gattcagcat ggcatctttt atagtgagga cacttaccct gattcgcatt tatttgcgga
     4921 tgcccattat ttagtgaatc agtatcagcc agactttttg gtcgtacatt cgatgaatat
     4981 tgatgatgtc ggtcacaaat ttaccgccga ttctcatgaa tatgcagcag cggttaatca
     5041 ggcggacaga ttattggcta tttaccttcc tgaatggctg caaaaaggtt atcaagtcgt
     5101 ggtgactgcg gatcacggga tggatgcgca cggactgcat ggcggcagct tggctgcaca
     5161 tcgtgaagtt cctttttatt tgatttcaaa taaacgccaa aagctgaagg aacaaactgt
     5221 accacagctg ctggcggctc cattgttttg ctggttatta ggaattgcac cgtcagagaa
     5281 aatgcaggac attcaagaat tgatgagggg atgaaagaga tgaaaaaacg cttggttctg
     5341 gttcctgcct cctttatctt ctgtgcattt ttactgctgc cgctgctttt tatggttctg
     5401 tgcagcttgc acagtgattt aactaaccag tggacattgg ccaattacag tgatattttc
     5461 agcaacaaat attacacaca ggcctttgtt aacagcgtac tgattgctgt cagctccagt
     5521 gtgattgggt taatcgggac gtttatttta tgtttatgct taatgaatct atcgactaaa
     5581 ttgcaggaga aaatcacctt tgtttccaat ttggcagcta attttgctgg gattccgctg
     5641 gcattctctt ttattatttt gttggggaat gtaggtgtgc tgaaaatgat tttgccattt
     5701 ctagcggact tcaatttgta ttcttggtgg ggattgacca tcacgtatac ctatttccaa
     5761 attccgttag gggtcttgtt tttgtatccc agcatgaagg aagtgaaaaa ggaatggctg
     5821 gagtccgcac aattgctggg cgcttcatca gcgtatgctt ggttcaaggt ggctttgcct
     5881 tttttgcgac catcgattgc cagcaccttt gtcatcttat ttgctaatgg catggggacc
     5941 tatgaaacag cgtatgccct gaccagcagt aatgtaaatc ttttaacgat tcgaattgcg
     6001 gctttagtgt cgggagatgt gttcgccaag cctaatttag gcagtgcgtt ggcagtcgct
     6061 ttaggtgtcc tgctggtaac ggcaatgttg attattcaaa agaaaggcaa ggtggtgcag
     6121 acgtgaaaaa gttaataaaa ccgattttgt tctgctttgt cctgtattac ttgctgccga
     6181 ttattggaac gattttgtat gccagctcca ctaaatggtc aaaatcgctt ttacctagtg
     6241 attttacctt gcaatggttc caacagctgc tgacggacag ggaatttatt gctgcggtgg
     6301 gtcgctcact tttattagca ggtgtggtct tggtgacgat tctgttgttg atgattccca
     6361 ccattatttg gattcatttg tatttccctc gtttgaatcg ttggctggaa aagctgctcc
     6421 tgctgccgta tgctttacca ggagtgattt tggtaacggc tttgcttcga acgtatgctg
     6481 aaactggtat cccaatgttt gtagtgttgg taggtgcatt gtttattacg gcgttgccga
     6541 ttgtctatct tagtcttaac aaccagatgc gcttaattaa tctgaaggaa ttagtggatg
     6601 cagcagaaac cttgggcgcc ccgatgtcta cgattattat ccaggtgctg tttccaaaca
     6661 tccgtatcgg tgtgacccta gtttctctga tgattttttc ttcggttttt ggggagtaca
     6721 tgctgacgaa cctgttgatt ggcggtcgat ttcagacggt acgcatctat atgctgcggc
     6781 ggatgaacga aaacggccat ttggctagcg ctgtgatggt tttgtatttg ctgtttatgg
     6841 tgggaattgc tttaactacc tttatgctga cttatcgaca aaagcaacag ctgtccctta
     6901 agcagacgag ggaagagaag cagtcgaaaa agaagccctt aaaggagttg tgacggaaaa
     6961 tgtttttaga aatacatgat ctcaatctgt tttttgatga aaaacagatt ttagctattg
     7021 atgaaattca gattgaaaag ggtgagctgg ttactttact aggtcccagc gggtgcggaa
     7081 agtctacgtt gctgcgctgt atcacgggat tggaacaacc aaagagcggt gagattgtgc
     7141 tggacaatga gaatattgcc gataagccta cgaaggatcg caatatcggc tttgtttttc
     7201 aacagtatgc attgtttcca actatgactg tttttgaaaa tgtagccttt ggtttgaagg
     7261 taaagaagct gagtcctgag gtgattcagg aaaaggtttt tgagatgcta tcattggtgg
     7321 atatgacgga gcaggcagat aaaaatgtcc agtttctttc tggcggtcaa cgacaacggg
     7381 tggcactggc tcgctcattg gtcactgaac cgaaagtcct gctgctggat gagccattaa
     7441 gtgctttgga tgcccgaatt agaaaacagc tgcagcggga tttgcgcgct atccagcaat
     7501 cgctagggat gacgatgatt tttgtgactc atgaccaaga agaggcgatg cgaatcagtg
     7561 atcggatttt tgtgatggaa gctgggcggg tggctcaggt ttccacctcg aaggaattgt
     7621 atcagaatcc tcagagccga tttgttgcgg aatttatcgg caactataat cgtttcgaat
     7681 ggtatgagct gaatcagtat acccacgagt tttccgctaa gggcaatcgc tgcatttatt
     7741 atttaagacc ggagctgatt caatttgaac cggttcctca gggggttaag attccggttc
     7801 tatggaagga atcatttatt ttaggcaaca ttcaacggta tgtttttcaa acgctggagg
     7861 gcaaagaaat tttagtggat cggttgaacg atcgggaaga gaaaattagc gatgccttat
     7921 acattcagct gaaagatatt ctagcaatcc ctgtggaaat tgatactcag cattttgcac
     7981 aacaaagcta gtgtatagtc ggcaaacatt tctggctctt tgtcaaatag gactgataga
     8041 gtgatataag aacatctaag gtagaattga ttttctgctt tagatgtttt tttatgttta
     8101 ataagtgatc agatgattga ttaagaaaat tctggttctc atcctaaccc gtaagctatt
     8161 cgtttcaatg ctttgatgtg ggtatgcaga ttttctattt tgccatttga gtaaggcaac
     8221 tctaaggcta aacgaatgcc ggattcatag tttataaacg ctttttttat atgatgcagt
     8281 tctcttggaa ccgacttggg cattgttttt acaagctgaa agaagatgtc cgcttctttg
     8341 tcataaaaag cttgaagaat ttcttgatag taagcataag ctattcgtag ttcatcagaa
     8401 gtagaaagta gacgttctac gacttcactt tcggttaaat aggcccaacc aaaattacgc
     8461 cattttgtcc atttatcata aacaagcgtg tcctgtcgtt tgatcagaag tcgagataaa
     8521 gccttcactt gtcgatagtg tttggcttgt attggatcgt ttcggtcgaa gcttttcatg
     8581 actcatagtc aaatatcctt aaaactgcga atgaggtgct tgacgatgtg aaagcgatcc
     8641 acaacgagtt tggcatgagg aaagcactct tttatcagca aagcataaga ggcgttcata
     8701 tctgttacga gtatttttac gtttaaacgt gcacgtcgtt caaattgcat aaaataagaa
     8761 atgagatctt tcttcttacg tgaaggaaga atatccagta atttcccagt atcgccatcc
     8821 atacaagaaa aagaataggg accgacttga tttttcaatg aacgaaattc atctaccaat
     8881 gaacagcgag gcaaccaatt tattttcacg tttgtcatgg ggagcagaga agcgagtgtt
     8941 cgcgaaacgg ttgaccaaga aacgccgcaa tgtttagaaa tcgtcgtcat tgcttgtttt
     9001 tcagtcaatt cctccaatat tttataatgt acttgtttgg cgataaaaca gtttttttca
     9061 acaatgtagg tattcgccgt aaaataggtg tgacagccac gacagaggta ccgttgcttt
     9121 gccaaagcaa gataagtccg ttgattcgca cacttattga gaaggaccag cgattttttc
     9181 tttccgttct taacgatcag cccatgttga ttcacctgtt tacactgagg acagggctga
     9241 atcgttttaa tgagcgtggc agaaacaacg atactgttga ttgaatgtat ttttttcttc
     9301 tcgacatgag tgatttgaat gtttccatct ttgattccta gtaatagttt gataagatga
     9361 agatcaggca tacagaacgc tcctttgatt gtgttgtggt gattcaattc taacggagct
     9421 tctgtatgtt ttgttttttt atgctcaaaa ggactgatag aatgatttac tatcagtcct
     9481 agaaattata gcgccacatt tctttcacct ttgacttttc agacggcagt ttttatgctg
     9541 atactgtaga gagagcagga cacagctttt gcttgttttg ggtaaggggg aaagaagatg
     9601 accaaagcac ttatttttga ttttgatggg acgatgattg acagtgaatg gatgttttac
     9661 gagagtattc atcactattt actgaagcct atttactgga gcagttcggc tataaaattc
     9721 cttttgcaga ctatcaacaa ggtgttggga cggcagcaga actgtccttt tatgaaggga
     9781 taagtcaagc ggttgggaga ccagtggatg ctgaacattt tgagcagcgg atcagcagca
     9841 ctggaaaatc ggagtagtct caattctgaa ctgcgggagc tagactattt ttttgaagca
     9901 catccgatga ttaaggaaaa aattgattcc tttgtgacga tcgatgatct tcaggtcggc
     9961 aaaccagatc cagaaggtta tttgttgtgc ttgaaacggc tgagggttga acaggctgtt
    10021 gtagttgagg atagtccagt cggtgcccaa gcagcaccgc atatgacagt tgatgtacaa
    10081 gcgttggatg cagatttttt cgcctttagc gggcataaaa tgtgcgggcc gacaggaatc
    10141 ggtgtattat atggcaaacg tcaattgtta gaacaaatgg agccagtcga attcggcggt
    10201 gaaatgatag attttgtcaa tctttacgat agttcatgga aagagctgcc atggaaattt
    10261 gaagctggta ctccgaacat tgcaggagca atcgattact tgaatgagat tggtatggag
    10321 aatattcatc gttatgagca agaactggtt gcctacgttt tacctaaatt acaagcgatc
    10381 ggcggtatca ccatttacgg accgcaagat cctgaacatc atactggcgt ggttgctttt
    10441 aatttggatg gcatccatcc ccgtgatgtg gcgactgctc tggatatgga aggtgtcgct
    10501 gtacgttcag gacaccattg tgcacaacgg ttcatcgacg atttctgttt gactaattgg
    10561 tcgtttaaac agtcgatgat aatgccgttt gagcggctcc aatgtcgtgg aatctttgag
    10621 taagagtttc caatatcgct tgagccgacg gtacttcttc tgattttcag attacgtgat
    10681 tgattttcgt acagttctat gagaatcgcc tgcttcagtt cctttgttag ttgatgatgt
    10741 tcatcaacta agtccgtttt cgcagaaaag gtctgcccac aatcatagca tagaaaacgg
    10801 gtacgtttta attctaaaat agtcaacttt tctttgactt tcaacatctg agtacgtgtc
    10861 gtataggaac cgttgcggat aatacgaatc gatccacagt gttcacaatg ggaaggtgag
    10921 tcatccaatc tccctttaat aaactgcgct tgaacgcctt tatattcttt ttcaatcagc
    10981 cattcttcat caaaaaataa atgtttatct gttaatccga gtaattttct agtatgatta
    11041 tccatagagc gactcctttg gtagggtgat gttttggtcg actaaatttt accaaaagtg
    11101 agtctctttt tgagttgaaa cgaagaaaaa gtgttagtag aaaatcgcct tcgattttcc
    11161 aacaacacaa aatattatag aagccttttt tctaaaggat ttcaaggtgt ctgtttgcat
    11221 ttattaaaaa aaagaaaaag tattggcaac agaaggcgtc accaacacta gatattatag
    11281 agccataata tgctgtataa tatctagtgt ataggcttaa gtggaggtat atatgaaaat
    11341 attaactgtt gaagatgata atatgataag agagggaata agtgaatatc tttcagaatt
    11401 tggatatact gttattcaag ctaaagatgg aagagaagcc ttgtcaaaat ttaatagcga
    11461 tataaatttg gttatcttag acattcagat accttttata aatggtttag aagtgttgaa
    11521 agaaattaga aagaaaagca atttaccaat tctaatcttg actgcattta gtgatgaaga
    11581 atataaaatt gatgcattta ctaatttagt agacggatat gtagaaaagc cattttcatt
    11641 gccggtc
//
LOCUS       DACDUU010000084        11529 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.084, whole genome shotgun sequence.
ACCESSION   DACDUU010000084 DACDUU010000000
VERSION     DACDUU010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.084"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            70..1758
                     /locus_tag="IWL30_002430"
     CDS             70..1758
                     /locus_tag="IWL30_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707153.1"
                     /translation="MKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLSRI
                     KGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLLNI
                     CTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYY
                     AYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFV
                     VVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDI
                     TTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLL
                     QFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVI
                     QSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKA
                     ADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIE
                     ITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            1745..3379
                     /locus_tag="IWL30_002431"
     CDS             1745..3379
                     /locus_tag="IWL30_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707154.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            3554..4387
                     /gene="msrA"
                     /locus_tag="IWL30_002432"
     CDS             3554..4387
                     /gene="msrA"
                     /locus_tag="IWL30_002432"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9707155.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            4467..5456
                     /locus_tag="IWL30_002433"
     CDS             4467..5456
                     /locus_tag="IWL30_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAP9707156.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            5745..6443
                     /locus_tag="IWL30_002434"
     CDS             5745..6443
                     /locus_tag="IWL30_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAP9707157.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            6593..7909
                     /locus_tag="IWL30_002435"
     CDS             6593..7909
                     /locus_tag="IWL30_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9707158.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            7926..9341
                     /locus_tag="IWL30_002436"
     CDS             7926..9341
                     /locus_tag="IWL30_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9707159.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            9806..10126
                     /locus_tag="IWL30_002437"
     CDS             9806..10126
                     /locus_tag="IWL30_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9707160.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            10152..10469
                     /locus_tag="IWL30_002438"
     CDS             10152..10469
                     /locus_tag="IWL30_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9707161.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            10498..>11529
                     /locus_tag="IWL30_002439"
     CDS             10498..>11529
                     /locus_tag="IWL30_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9707162.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVL"
BASE COUNT         3851 a         1813 c         2279 g         3586 t
ORIGIN      
        1 caaaacttat taaaaaaaga caattctagt atactgaaaa tagaattaaa aaaggagaac
       61 aacaaatcta tgaaaataag taaaaaagca tttcttttag tagttcttat cttacttagt
      121 actttatatt cagtaaattt tatgagaaat gctcaagaaa tttatacaac aggtgattta
      181 tcttttcatc tttcacgaat caaagggtta tcttctattt ttgaagggcc aatcaactat
      241 acaacattta acaattatgg agatggactg aactattttt atccttttct tacaataatt
      301 cctgcagtcg ttttttatgg aatttcaaat aacttaatat taagttatgt tctatatatt
      361 tggttattaa atatttgtac gattctgatt agtttttatt atggagaaaa gttttttaaa
      421 aagatagatg ctgctttttt atttagttgt ttatatactt ttgcaactta tcgaacagtt
      481 gatatctatt atcgatccgc aattgcagaa gcaatcgctc ttacgattat cccagctgtt
      541 ttatattatg cttatggatt gatattcaat agagaaaaaa aagtcatcgc tttatctgtc
      601 agtataagtc tacttgtgta tactcatgtc ttaactacac tattgtgtag tgcattgatt
      661 gcttgtatga ttatagttcg tttgttttta agcaagcaaa taaaaaacga tataaaattt
      721 gtggtcgtga atttagtaaa ggctgttttt ttgacttgtg cattaactag ctatttttgg
      781 tatcctatgt tgcagcagat gaattatcaa gaaatacaca gtcccttcaa aacagatttg
      841 cagctagaag cactttctat ttcagatagt atcattcaag cgttaaataa cgatattaca
      901 acatacacta tggggttatt aggattggtt gcgttgattt ttcctttatt catactgaaa
      961 gaaatggaga aaaaagaaaa aatcatctat gttggcgtag tttctacatg ggtatgcgta
     1021 actaatttat ttccatggtt tttgtttcaa aaaacaccga taagcttact ccagtttcct
     1081 tggcgtatct taggtatcca aatctttttt tcttcaatga tcataacgct tgtttttatg
     1141 aaaaagacta tgaataaaaa atattcatgg ttaatcattg gcgctactgt ggtgtttcta
     1201 ctcgtcacgt ctatagcagc aaaagaaaat tacgcaaaag ttatccaatc aaaacacggt
     1261 catattgtta tcaatgaaga aacactccca cgttatacaa catcagatat gggaggatta
     1321 tacgattacg ccccaacaga agcgataaaa gaaaggcctc acttagaaaa acatgaagta
     1381 aaagtaggag aatcgtggga aaaagggaag tataaagctg cagataataa cattacttac
     1441 acagtggcaa gcaacaaaaa gcaaaaagtg acagtaccag tatatgctta tttaggtaca
     1501 caggtaaaag taaacggtaa agtcgtaaat gactcttata aaaaacatgg cttagtgaat
     1561 gtggatgtaa atgctggaga aaataagata gaaattacaa caaaatatac tccaactgct
     1621 ctcatggctt tatggatcag tattctgact tattgtttag tggtgtattc aagttttaaa
     1681 cgatttgcac ctaataagtt gaaagattct cgatatgtga ctaaaaagga gataaacaag
     1741 aagaatgaaa ataaataaaa aagtagtaat attgagtgga ttaattatag ttagtagtat
     1801 atattctttc atctttttca aaaatacaca gagtatttat accactggag atctttcttt
     1861 tcacttatct cgaatcaagg ggttatcgac tatttttacc agtccgatta attatgagac
     1921 attcaattat actggatatg gtgtaaatta tttttatccc tttctgactt tttttccagc
     1981 tgtaatgtta tattggatga caaaaaattt gattgtaagc tatattattt acgtttggtt
     2041 attgaacttg tgtacaacac tcgtagctta tcattatggc gagcggtttt taaaacaaaa
     2101 aaaagcggca tttttattta gttgtctcta tattttctct gcttatcgga cagtagacat
     2161 ctactatcgt tccgcaattg ccgaagctat cgcaataaca cttgttatcc ctgtattatt
     2221 ttatgcttat caaatcatct ctggaaaaga agaaaagtat ccgtctgtaa agctagcatt
     2281 gagtatgagt ttgctagtgt acagccatgt actatcaaca ttaatgagta cagcattaat
     2341 tatcattttt atttttattc gtttggtttc taaaggtttc aagaatgcag attttattgc
     2401 tatatttaaa aaattattta gtgcggcagg aatgacattg gttctgacga gtgctttttg
     2461 gtatccgatg tttgaacaaa tgctctacca aaaaatcaac aaacctagcg tgacaaattt
     2521 atatgctcat gcatctaatg tgttcgatag tctgactgaa gctatgaaca acgacttaac
     2581 tacttactca atgggactag ttggtctgtt atctttatgt attcccctga ttttatttaa
     2641 aaaattaaca aacatagaaa aaaagattta ttatggtacg tgtttgactt ggctagctac
     2701 aactagtttg gtgccatggt atcttctgca aaatacgcca gccaaattgt tacaatttcc
     2761 ttggcggatc ttaagcttgc aaataatatt ctcttctctg atattaacga tgatattttt
     2821 caaaaataga agatacaata agacaagaga actattttat ttatttatga ctgttttgtt
     2881 attaatcact ttaactgttt ctagtaaaaa aaatttcaat caaaaaataa tttctcagcc
     2941 gggacatatt gttgtaaaca aagaaacagt agaggcgtat accactaaag gaatggatta
     3001 ttttttatat gattattctc ctatcgcttc attggagaat aaggagcata taataaaaca
     3061 cgaagcaaag attggcagtc aatgggtaaa ccttccttat aaaacaactg gtagttctat
     3121 catatataga gtaactaatg agaagagcca agtaatcaca cttcctgtat atgcttattt
     3181 aggaacgaat gtagaaataa atcatcaacc tgtaaaggag caggtgaatg ctaatggatt
     3241 ggtacaaata agcggagtca aaggagttgc tgatattaaa gtaagcacaa gttatacagt
     3301 aacgactatt atttcttatc ttgttacatt aactgcatat ttatgtttat tttttaaaat
     3361 agttttccat aaaaggtaaa ttaacttttc atttataaaa taaattgatt aaaaaggaga
     3421 atctgtcgat aaggattctc tcttttttat acgaaaaatc tgtaggggga tgaagtaatc
     3481 gcttatttcc tcactttatg ataaattaca gctaaagtca ctagcttgtc ttcttcggaa
     3541 aggaggagaa tctatggatg aggaacaaat attcattcaa acactttata atctgatttt
     3601 gaatccgaat acacgagatt gggaacgaaa agtattgatt cagacaaaga atgacataag
     3661 agaaaatata tccgttaaag aacagttatc aaaactagaa gcaacactac gtccattagc
     3721 tatccgaatg aatctgacgc ctgatgtaat ggacttttat ttattgatta cggaaggatt
     3781 tgataaggag caaaaatatg acttttccaa gcatgccatg caagatgctg actatcaaga
     3841 acgtgcagta tttgcagggg gatgtttctg gtgtatggtc gagccgtttg aatcgaaaaa
     3901 agggatccta tctgttttgt caggatacac gggcggacat gttgaaaaac caaattatga
     3961 tcaagtaagt ggtggttaca caggtcatgt ggaagctgtg gagatcattt atgacacaag
     4021 agaaataagt tatagcgagt tgctgacaat ctattggcaa attactgatc ccacagatac
     4081 atttggacaa tttcaagata gagggaagca atatcgtcca gtgatttttt atcaagtcga
     4141 acgacaaaaa gaattagcgg aacaatcaaa acagaaacta gacagttcag gaactttcca
     4201 ccaaccaatc gtcacaaaaa tcgaaccagc cggtacgttt tggccagcag aaaactatca
     4261 tcagcaattt tacaaaaaac aaccgaaacg ttataaaaaa atccagcaag cacgtaatca
     4321 atttctgatc tatcaacgag taaaaaacaa atggcaaaaa aatatacgaa aaaatcattt
     4381 tgattaactc cttttcttta aaatgtagta aactaaaaat agaaaaaaga aagcgtttta
     4441 aaaagatttg atgaggagga gaaaaattgt atattgcaga tagtgcacgt tatgaaaaaa
     4501 tggaatatcg taaatccggt cattcaggtt tgaaattacc gattttatct ctcggattat
     4561 ggcaaaattt tggtgattat gatcctatcc ataatcaaag agagatttta agaggcgcat
     4621 tcgacatggg aattacccat ttcgaccttg cgaataatta tggaggccct gctggagctg
     4681 cagagaaaaa ctttgggcgt atttttagag aagattttca agcctaccgt gatgaactga
     4741 ttatttctag caaagcaggt tatcatatgt gggaagggcc atatggggaa tggggctctc
     4801 gtaaatccat catttcaagt tgtgaccaaa gtctccaaag aatggggcta gattatgttg
     4861 acatcttcta tcatcatcgc ccagaccctg atacaccttt ggaagaaaca gcagaagctt
     4921 tgatgcaact tgtaagacaa ggaaaagcat tatatattgg tatatccaac tataatggag
     4981 aagatacaaa aaaaatgact gaaattttaa aaagaaagga agcaccgttc attattcatc
     5041 agatgcgcta caatatgttt tctcgtgcac tactagaaga cgatctatct ccagtattag
     5101 aagaagaagg gctaggggca atcacgttca gtccgcttgc tcaagggttg ctgacaaatc
     5161 gttatctaca tggtattcct gaagattcta gagcacatcg taaggaaata ccattccttt
     5221 cagaagaaca agttggatca actctggaaa aaatcaaagc actgcaaaca atcgccgtat
     5281 cacgaggaca atcattagcg caaatggccc ttgcatggaa tttgcgacaa aaatctgtta
     5341 caagtgtact ggtcggagca agtcggttaa gtcagttaca agaaagtgtt cgaatgatgg
     5401 ataatttaga tttctcacca gaagaagaat tgagaatcga ccaaattcta gaatagaaac
     5461 gatgtaatca aactttttat ccatatatta aaaaaaacaa aagatgacat caagaatcga
     5521 atgaagatcg tctgttattg aacatcatat tttttgtcgt tcaaaataaa aatgatcgaa
     5581 ggctaggagt gaattttatc ctagtctttt ttatggtaaa tatcttcttc gtttgtatgt
     5641 ctatgaacgg attaatcaat tttgtataca aaaaatcatc agatgattca aacgtgttat
     5701 aataattacg aaaaaataca tcagatgaaa aggaggtatg tggtttggaa gaaaataaaa
     5761 ccctagtaga agtaactact gacaagatcc ttagatattt aaaggaaatg cagttgcaag
     5821 taggagataa actgccgaat gaatatgaat tgtccgatta tttagaggtg gggcgaagca
     5881 cggtgcgaga agctgtgcgg gctctagctt ccaggaatat ccttgaagtt cggcaaggag
     5941 cggggacatt tgtcagtgat aagcgaggga ttgccaatga cccactcggt ttttctttga
     6001 ttgaagatca ggatcggatg atacgagact tatttgaact gagatattta ttggagccta
     6061 agatggcagg tttggcagcg gaatatgcaa cgaagaatca aatcgtccaa atgaaaaaat
     6121 taacgcaaaa gattgaaact tcttttcgag aaggcacaca agaacatgtt caattagaca
     6181 tcgattttca tacgatgatt gcagaagcta gcggaaacgt agccttgctg catattatcc
     6241 caatcattaa tgaatcaatt gctttgttta ataaaaatta tgatatccag catctgaaaa
     6301 tcgagacgat cgatattcat acagagatcg ttcaggccat cgaaaggcgg aatgcacttg
     6361 ctgcgtttga tgcaatgact gtccatatgg caataaatcg gagagaatta cagaaaataa
     6421 taggagaaaa agaaactaat tgacagttca tctgatgtat tttatgataa atacatcaga
     6481 tgaatttttg ttttatattt ggtaaaaaag aggagtaaat agtttctgtt catcgaaatg
     6541 taagcgctga ctaatagaca aaaagagatg acgtcaagga gaagaagcag gaatgagggg
     6601 atctggaatg agttttatgg ataaattagc caatgtttta gggaacgtgg caacgagaat
     6661 caataattta aggtatatca tggtgataaa aaacgcattt gctgcattga ttccggtaat
     6721 tattacgggt gcttttggca cattgttttc agcaatggtc tttgactcgg aaaatggctt
     6781 agcacagata agtttcttgc gttttttaga agaattaaag cctatcgcat ctgccatcag
     6841 ctatgtcaca ctaagtttct tgaccatcta tgctgttttt ctgataggga tcgaattagc
     6901 caagctgaat aaagtggatg gtgtattccc tggtatcatc gcagtgatgt cttatttatc
     6961 tgttaatcca actgtttatg aattagtgaa tgaaaatcag actgttatag tagaaaacgt
     7021 attggctaaa caatatacag atacgaaagg gttatttttg ggaatgttgg tctccatcct
     7081 ttctattgaa ctttattgct ggttgaggaa acagaagaaa ctacagctga gaatgcctga
     7141 taccgtgcca acgaatgttt ctgctagttt ttctgcttta ttcccgacga ttctgactgt
     7201 atcgggtatc gcggctgcgg gatttatcat caaagaactt acaggaatgt acctttatga
     7261 tatcatttac aatgtcgtac aacgcccatt agaaggtgtc gtacaagggt taccgggtat
     7321 tttgctattg atgtttattg cgcaagtctt ttgggtgatt ggtatccatg gaaatcaaat
     7381 ggtgaagccg atacgggaac cgttgctttt agcggcaatt gctgtaaata cggaagcttt
     7441 tgaagctgga aaagaagtac cgaacattat tactatgcca ttttgggata tgtatatgag
     7501 tatgggcggg tctggagtca cgatcggttt actgattgca gtattgttag ttggaaaacg
     7561 ggaagatatg aaacaaatca ccaagttatc ccttgctcct gggatcttca acatcaatga
     7621 accagttatt tttggtatgc cgatcatgct gaatccaatt ttagctattc cgtttatctt
     7681 aacgcctctt atcactggaa cgattggtta ttttgcaacc gctttaggat ttgcggcgaa
     7741 agcagttgta atggtccctt ggccaatgcc gccgatcgtc aatgcttatc tggcaacagc
     7801 aggaaatatc ggtgcagtta ttacgcaact tgtttgtatt attgtttcta ttttgatcta
     7861 tctgccattt gtaaaagtag caaatcgtgt ggaaaatgat gggatctaaa aaacaggagg
     7921 aaatcatgaa aattactatt ccaaaaaatt ttattcttgg tgctgcatct tctgcttggc
     7981 aaacggaagg ttggaaagga aaaaaagaag gacaggattc gtttatagat agctggtata
     8041 aaaatgaaca ttttgtttgg cataatgggt atggcccagc cgtagcaact aattttatgg
     8101 aacaatacca gcaggatgtg gatctgatga aatccatcca tctgacacat tatcggactt
     8161 ctatcaattg ggctcgtttt tttactgatt atgagaactg tatagtagat gaagaatatg
     8221 ctcagcatat aagtgaaatg atcgactctt tgatcgaagc agacgtagaa ccgatgattt
     8281 gtttggaaca ttatgaagtc ccagcttttc ttttggaaaa atatgatggc tggtcttcaa
     8341 gaaaagtagt tgatttttat gctaagtatg ctcaaattgc ctttgatcgt tacggtgata
     8401 gggtaaaaaa ctggtttaca ttcaacgaac caatcgttcc ccaaacgcgt tgctatttgg
     8461 atgctgttcg ctggccgcat gaacaaaaca caaaaaagtg gatgatgtgg aattatcata
     8521 aagttttagc aacggctaaa gcagtagaga tttaccgcag cggaaattat ggtggaagga
     8581 ttggctgtat tttaaatcct gaaatggtct atgctcgatc caattctcca gaagaccaaa
     8641 aagctgctta tatgtatgat ttgttttata atcgagtatt ttttgatccg atggtaaaag
     8701 gatactacac agaagagttc ctggagttgt gtcaaaaaca atcgatttat ttcgatcctg
     8761 caacagagga tttagaaacg attcagcatc atacagtaga tttccttgga atcaatcaat
     8821 attatcctaa acgcgttcgc gcccctcgtt atcaatggaa tgaagagaca cctttccatc
     8881 ctgaacgata ttatgaagtc tttgatttac ctgggaagaa aatgaataat tctaggggat
     8941 gggaaatcta tccgaaaatc atgtatgata tagccatgta tctaaaagaa aattatcata
     9001 atatcccttg gctgataaca gaaaacggta tgggaagaga atatgaagaa cagtatatgg
     9061 atgagaaagg aatcgtgcaa gatgattacc gtatcgaatt tatcggacag catttgcatt
     9121 acttgatcaa agcaacagaa gaaggcgcaa attgcgaagg ctatatgtta tgggcattca
     9181 ctgactgtgt gtcaccaatg aatgcattta agaatcgata tggattagtt cgaatcgaat
     9241 tgaacgatga acgaagcaga acattgaaaa aatctgcttc gtggtatcag actttgattg
     9301 ataagagaga attggatttg cctgacgaaa tctataaata aatagaagtt ttttgtagat
     9361 gttcattgta tttactggtt atttgaaata aaagaggttg tgaatccagc gttttgagct
     9421 aatacccagg gtcagctgat tgaacaccgt ttattgagag gttgtgaaaa agctgtcctg
     9481 catcaagtaa taagaatagc aaagtaaaaa caacatctta tgtttttaac ttggttggac
     9541 ttattaccgc agatgcaagc ttttgaatac cgtttatttg aaaaaagttg tgacaagttt
     9601 tgtgtcacaa ctttttttgt gcacgttaaa agaaaaaagg taataaaaat taatttgtgt
     9661 gtttagtgtg cgctttcttt ttttagtgtg tatgcaactt ggtatagacc ttaaatgtga
     9721 tgatatcaac tttttaaagc gcttttaaag ttggcacgct acttgcttat atacaagtgt
     9781 aagtatagaa aggaacgtgt ttaaaatgga aaagaaaaca atcatgcttg tatgttcagc
     9841 tggaatgagt acaagtctcc tagtcacaaa aatgcaaaag gcagcagaag caaaaggaat
     9901 ggaagcagac atctttgctg tatctgcttc agatgcggat aatcaactag aatctaaaaa
     9961 tgtagacgtt ttacttttag gaccgcaagt acgctttatg caaaatcaat ttgctgaaaa
    10021 gctagcacct aaaggcattc ctttagatgt aatcaatatg gctgactatg gcatgatgaa
    10081 tggtgaaaaa gtactcgacc aagcagaaaa actaataaac aaataataaa aattatcgga
    10141 ggatacaata aatggaagat caaaaaaatc tagaagccat catgggtctg atcatgtttg
    10201 gcggaaatgc aaaaagcgat gcaatggaag cgattgctgc tgcgaaaaaa ggtgatttca
    10261 ctttagcaga tgaaaaaatc aaagatgcag aggaatcttt agtccaagca catcactctc
    10321 aaacagagat gctgacacaa gaagcacaag gaaatcacgt tgaagtcacg cttttgacgg
    10381 tccacagtca agatcatctg atgacatcta tagcttttac agatttagca aaagagatca
    10441 ttgatctgta tcgtcgattg aacgcgtaat ctacagtaaa gaaaagaggg aagatttatg
    10501 gataaatttg ttgcatttat ggagaaacat tttatacctg tagcatcaaa aattggtgca
    10561 caacggcatt tagttgcgat tcgggactca tttatggtga gtatgccgtt aatgatctta
    10621 ggagcattag cagtaatgat caataactta ccgattcccg ggttccaaga attgatgaac
    10681 tcaatttttg gtggtgaatc atggaaagga ttcggggctg ctgcttggaa cggtacattt
    10741 gccattcttt ctgtcttgat tgctttcttg cttgcttatc atttagctaa cggctaccga
    10801 aaagacggtg tttccgcagg tgtgatttct ttaggctctt tcttcgcttt aggcggagca
    10861 ttaggtatga gctcaaacgg tttgtttatt gctattattg ttgggattat ttcaacagaa
    10921 atctttgtac gtttaagcgg aaatgaaaaa ttgatcatca aaatgcctga tggtgtgcca
    10981 ccggcagttg caaaagcatt tgcttcatta ctaccagcaa tgatcactat ctctgcgttt
    11041 gctttagttg ctgcaatttt tgctgcattc ggtgttgatg atatcgtagg ttctttctat
    11101 acagtggtcc aagaaccatt tatgggactt gcaaactctt atccatctgc cttactgctt
    11161 gcatttatca caccattctt atggttcttt ggtttacacg gagcgaatat ggtcgatcct
    11221 ttgatgcaaa caatcaatgc gccagccatt gaagcaaacg ttaatgcaat cgcagctgga
    11281 catagcgcac cgtttatcgt gaacaaacct ttctttgaca gctttgtcaa cttaggagga
    11341 acaggtgcaa ctttaggttt actgttagct atttatttag taggacgtaa aaacaaacca
    11401 tatatggttg taacaaactt gtcgattgcg ccaggtgtct tcaatattaa tgaaccaacg
    11461 atgtttgggt tacctatcgt gttgaacccg atcatgttca ttccatttat tttgacacca
    11521 atggtctta
//
LOCUS       DACDUU010000085        11483 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.085, whole genome shotgun sequence.
ACCESSION   DACDUU010000085 DACDUU010000000
VERSION     DACDUU010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.085"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(96..581)
                     /locus_tag="IWL30_002440"
     CDS             complement(96..581)
                     /locus_tag="IWL30_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAP9707163.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            1061..1702
                     /locus_tag="IWL30_002441"
     CDS             1061..1702
                     /locus_tag="IWL30_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9707164.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            1721..2356
                     /locus_tag="IWL30_002442"
     CDS             1721..2356
                     /locus_tag="IWL30_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9707165.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2374..3189
                     /locus_tag="IWL30_002443"
     CDS             2374..3189
                     /locus_tag="IWL30_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9707166.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3508..4944
                     /locus_tag="IWL30_002444"
     CDS             3508..4944
                     /locus_tag="IWL30_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9707167.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5133..7580
                     /locus_tag="IWL30_002445"
     CDS             5133..7580
                     /locus_tag="IWL30_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP9707168.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7810..8877
                     /gene="rlmN"
                     /locus_tag="IWL30_002446"
     CDS             7810..8877
                     /gene="rlmN"
                     /locus_tag="IWL30_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP9707169.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(9070..10173)
                     /locus_tag="IWL30_002447"
     CDS             complement(9070..10173)
                     /locus_tag="IWL30_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9707170.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10726..11274
                     /gene="aac(6')"
                     /locus_tag="IWL30_002448"
     CDS             10726..11274
                     /gene="aac(6')"
                     /locus_tag="IWL30_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP9707171.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         3813 a         1940 c         2482 g         3248 t
ORIGIN      
        1 tacattgttt gattggccaa aagaatttct gatatatttt gtgctaaata aagaaaaaga
       61 cgcttgaatt gggaaataag aaaactactc tatcattaca aattttcata aactggcaga
      121 tgttctaagt aaacaagtat cttttcaaag tcggaaaaat ccataatcaa cgtagtggta
      181 ttgatatttg gatgaaagcc tacggtatct tgacgatcaa tagcagcatc gatcacgact
      241 tggatttttt tctctttgtc gtgcatcaat gctaatggag tcactgtacc tgctggaacg
      301 cctaggaggt caggaagctg ttcttcagaa acaaaagata agcgcttttc ttttaattgt
      361 tcagctagtt gttttaaatc ggcgtgcttt tcatcaggca agatgacaag atagatttgt
      421 tttccttttc ttgatttcaa taatagattt tttacttgcg gaccaggtag ggaaaaaggg
      481 acatttttga ctgacgtaat cgcaggatga tcgacttttt gaaaagagat acccagtttt
      541 tttagtagtt cataagcttc ttgttcggtt gcacgactca tcgcgcatca ccctcctttt
      601 tttattagtg tatcataaaa aagagttgac aaatgaaaaa taatcatgta aattacacgt
      661 aaagttaaaa caaatgacat cgaaaagaag agtagccgtt aggtgttttt agagagtctc
      721 tggttggtgg aaagaggcag cagttaaccg tgaaacacat cttggagtcg attttctgaa
      781 gcgtatagta ggaaaatccg gggagcccgt tacagctgcg gtcgttgcta gatgaccaga
      841 taaggctggc ttttccgtga ggagtcggta aaaaaaggtg gaaccacgtg attaacgtcc
      901 tttgatcttc ggatcaaagg actttttgtt ttcatctgaa cggcaacgac tgtgtgaggt
      961 tttttctgca aggaaaaaat aaacagaggt ggaaccacgt ataatacgtc ctcttggcat
     1021 ttgccaagag gattttttta ttttaaagga ggaaaagaag atggagtatt tgattgaagt
     1081 tatgccgcaa ttattagtag gagcactcac aacattgaaa ttatttttat ttaccttgat
     1141 tggctcatta ccattaggga ttgttttagc attaggattg tctttgcatt ttaagccgct
     1201 acgctacttg cttcaaacat atgtatgggt tatgcgggga acaccgctgt tgttgcaact
     1261 gatttttatt ttttatgggt tgccgacgat tgggatcgtt tttgatcgtt tcgaagcagc
     1321 actcattgcc tttatcttaa actatgcagc ttattttgca gagatctttc gtggagggat
     1381 acaatcgatt cctcaaggtc aatatgaagc agcgcaagtt ttgcaattaa gtccctttca
     1441 aacggtgacg aaaatcgtca tcccacaagt gacaaagatt gttttacctt ctgtagggaa
     1501 tgaagttatc aacttggtca aagattcttc cttgatctat atattaggtt taggtgacgt
     1561 aatgcgtgct gggaaaatcg cgatggaacg cgacgcgact ttactgcctt tgctaggcgt
     1621 ggcaatcatc tatcttacat tgactggtat tctaacaatc ataatgaaac aagttgaagg
     1681 aaaattaaat tattaccgct aactgatagg aggaagaaat atgattgaat tgaagaaagt
     1741 gaacaaagca tttggtgaaa aaaaaataat caaagatttg aatctaatca ttccagatgg
     1801 gaaaacatta gctatcgtag gtccttccgg tggcggtaaa accacgcttt tacgtatctt
     1861 ggctggctta gaaagttctg acagcggaga attttattta gacggtgtac cgttcaaccc
     1921 attcgagctt aaaatgaaag aacaagttgt aggagtcgta ttccaagact tccagctatt
     1981 tccacatctg aatatttttg aaaacattac cttagcacca aaaatggtat tgaaacagac
     2041 aaaagaagaa tacaccaaaa aagcagaaga attagcggct tcattaggga ttagtgattt
     2101 attgaatatc tatccttatc agctttctgg cggacaaaaa cagcgggtag cgattgccag
     2161 agcattggct atgaatccac gggtattggc ttatgatgaa ccaacaagtg ctttagatcc
     2221 tgaattacgc cagcaagtcg agcgtttgat cctatcattg aaagaagatg gggtaaccca
     2281 aattattgtc acccatgatt tagtatttgc agaaaatgtc ggtgatcaat tactaaaagt
     2341 ttccccaatt cattaagaaa ggagaagata gagatgaaaa aaaccagttt tctattcgct
     2401 ttgatcgcag gtcttcttct ttttgcaggc tgttcgaaca aaaaaacttc agcagataat
     2461 tgggcgacta tcaacgaaga gaaaaaaatc gttataggtt tagacgatac atttgttcct
     2521 atgggattta gagataaaga cgggaagctc acaggatttg atattgattt agccaaagca
     2581 gtctttgaac aatatgggat tacggtagac tttcagccca ttgactggtc aatgaaggaa
     2641 tttgaactga acaatggaac gatcgatttg atatggaacg gctattccaa aacgccggca
     2701 agagaaaaga aagtgcaatt tacaaaacct tatatggaaa acgatcaagt gctgatcaca
     2761 cctaaagaat caggaatcac aaactttgga caaatgaata ataagagatt aggtgcgcaa
     2821 aatgggtcat ctggttatga tgtatttacg aaacaaccag aagtattaaa agagatagtc
     2881 aaaaaccaag atgctgtttt gtatgatagt tttaatgaag cattgatcga tctgaaaagc
     2941 agacgggtcg atggtctgct gatggataaa gtatacgcag actattattt gaaacaaaga
     3001 aacgaactag aagacttcaa cataacaaaa ggttcttatg agagtgagga ttttgcagtt
     3061 ggtgcacgca agtcagatag tacattagtg aaaaaaatca atgaagccct agatcaacta
     3121 gagaaaacag gtgaattcca aaaaatatcc gataaatggt ttggcgaaga tgttacgccg
     3181 agaaattaac aaatagggga ctgtgacaaa agacgtgtca cagtcccttt ttccgaataa
     3241 acagtgttct cctgccaact ccttgtgact cggagctgaa tggcaggctc acaacctctt
     3301 ggtaaacggt gttcaaaagc cagtttttcg gtattaagcc aaatttatgc aaaaatgtag
     3361 aaagccattt tcacaaaatt tttcttacta ctcgaaacta agcgactttt tcacaacctc
     3421 ctttacataa ttttattttg ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat
     3481 tgaaggttat aggaaaggaa gagatgaatg gaacacaaag aacttacctc atttccaaaa
     3541 gattttctgt ggggctctgc ttctgcagcg tatcaagtag aaggagcttg gcaagaagat
     3601 gggaaaggag aatcagtctg ggatcgattt gtacggatac ctggaaagac atttaaagga
     3661 acgaacggtg atttggcagt agaccactat catcgttata aagaagatat tgctttaatg
     3721 aaagaacaag gtctgaaagc ttaccgcttc tctgtagctt ggacacggat ttttcctaat
     3781 ggacgaggag aagtcaatca agctgggctc gctttttatg agagattgat cgacgaattg
     3841 attgaaaacg atattgaacc tatgttgact ttatatcatt gggatcttcc acaagcattg
     3901 caagatgaat ataatggttg ggaatcacgt cagatcattg aagatttcac tcattatgca
     3961 gaaacattat ttgaggcatt ccgcgggaaa gtccattact ggattagttt gaatgaacaa
     4021 aatatcttca cttcattagg ttatttgtta gcagcacatc cgccaggtgt gacagacccg
     4081 aaacgaatgt atgaagttaa ccatattgct aatttagcaa atgcatctgt catcaataaa
     4141 tttcacgagt tagaaatgcc tggaaaaatc ggaccaagct ttgcgtatac gccaaattat
     4201 ccaattgaca gtaatcctga aaatgttctg gctgctgaaa atgcagaaga cctgatggcc
     4261 aattattgga tggatgtcta tatgtgggga aaatatccga ttgctgccat gaggttctta
     4321 gaagaaaaag gatgggcacc aacgattgaa gctggcgatg cagaattgct agaatctgct
     4381 aaaccagatt ttcttgggat caattactat caaacggcga cgaatgcatt caatccatta
     4441 aacggagttg gtgctggaaa aatgaatacg acaggaaaaa aaggttcatc agaagaaact
     4501 ggagttcctg gaatgtataa aaaagttgag aatccatttg tcgaacgaac aaattgggac
     4561 tgggagattg acccagaagg attaaggatc ggactgcgtc gtatcacaag ccgttaccgc
     4621 atgccggtac tgattacgga aaatggctta ggagagtatg acaagttgac agaagataaa
     4681 caaatccatg acgactatcg aatcaattac ttacaaagcc acgtcaaagc catcaaagaa
     4741 gcaatttcgg atggagcaga agtcctaggc tattgcacct ggtcttatac agatttgctt
     4801 agctggctga acggttatca aaagcgatat ggatttgtat atgttgatca agacgaaaca
     4861 caagaaggat ctttggaacg atataagaaa gacagttatt actggtacca aaaactgatt
     4921 gaagaaaatg gagagaacct ttagttcaca tacttaaaac aagaaggtac ggctggatat
     4981 ggccgtgcct tttcttgata aaatcattta gaaattaaga aaaactgtaa aataagctga
     5041 aaaatgaaag aaagcgtcag ttctttcaaa caaaacttta gttcccttac ttttttttgt
     5101 ataatgaaac ttgatgaaat gagggataat aaatggcaca aaagaaacgg cctactaaaa
     5161 aaaggaaaaa gacgaaaaaa acgaaaaagc aaaaacagca gcaggaacaa atgctgacga
     5221 ttggtgttgg gtttgccttg attttatttg ctgtcttcgg ctttttaaaa ttaggttttt
     5281 taggaatatt gattgcaaac ggctttcgga tcattgcagg aaatacttac caaatcctct
     5341 gtcttttatt agccgtatta ggattttgga ttgtcattaa gaatacagag ttttcaattg
     5401 gtaaaaaccg acgctggttt ggcggcatac tcttttattt cggtatctta ttattgttac
     5461 atgcccattt atttggcaaa ctccatactg gggaaccaaa tatcatgggg acgacatggg
     5521 atttgttagc cagtgatata aaacaaagtc aagtagacaa taacgttggt ggtgggatga
     5581 ttggagcaat cctttatcat tttacttact tcttgattgc acagcctgga agttatttag
     5641 cagcagtgct attactggca ggaggagcat tcctgttcag taatctggaa ggctatcagc
     5701 tgttaaatgg tatccaatca attggagaac gagtacagga attattggaa ggtgatcctg
     5761 ccaagcaagc tcgaaagcaa gcggcaaagg aagagagaat gaaacagcgc gcagaagcga
     5821 aagaagcaag gcgcttagct gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga
     5881 agaaaaaagc ggtgagccag caaagggaaa atactgctga tgaatggcag gaaccagaaa
     5941 atacagagcc ggagcagcta agttttgttc caatcgacag ttttcaagaa aatatccatc
     6001 ctgcgaatct agagaaacca gtaccagata caccaaaaca gaccaataca gcagaaggtt
     6061 tttcagatga gttgccagag gatgacggta cctcattaga atttgagatt gaagcagaac
     6121 aggaaaatca agactatgaa ttaccgacag ttgatttgct tgattctata ccaactgtgg
     6181 atcaaagtga tgagtataaa aaaattgaaa aaaatatcgg cgtattagaa caaactttcc
     6241 aaagtttcgg tgtcgatgcc aaggtcgtca aagccagctt aggaccatcc gtcacaaaat
     6301 tcgaagtcca gccggctgta ggggtaaaag taagtaaaat tgtgaactta acagatgata
     6361 tcgcgcttgc tttagcggcg aaagatgtcc ggatggaagc accgattcca ggtaaatcac
     6421 tgattggtat tgaagtacca aatggaaaga tcagtatggt ttcctttaga gaaatcatcg
     6481 aagcacaacc aaaccaccca gataaattac tagaagtacc tttaggacgt gatgtttctg
     6541 gacgggtaca aacggctgat ctttcgaaga tgcctcactt actcgtggct ggttcgactg
     6601 gtagtgggaa gtcagtagca attaatggga ttatcacaag tatattaatg cgtgctaagc
     6661 cccatgaagt aaaactgatg atgatcgatc cgaaaatggt tgaattgaat atgtacaacg
     6721 gcatacctca tctgttgacg ccggtcgtga cgaaccctag aaaagcagcg caagccttac
     6781 aaaaagtagt acaagaaatg gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa
     6841 atatttctgg ttacaatgaa tttgttcaac agaaaaactt ggaaaacgga acgaaacatc
     6901 caaccttacc attcatcgta gtgatcgtag atgaattagc tgatttgatg atggtagcca
     6961 gcaatgaagt tgaagatgcg attattcgtt tagcgcaaat ggctcgggca gcggggattc
     7021 atatgatcct agctactcaa cgtccaagtg tggatgttat cacaggaatc atcaaagcaa
     7081 atgtaccttc cagaatggca tttgcggtat caagcggtac agattctcgg acgatcattg
     7141 attctaatgg ggcagaaaag ctcttagggc gaggagatat gctattcttg ccaatgggtg
     7201 agaataaacc gatccgtgtg caaggagcat tcatttctga ccacgaagta gaacgggtcg
     7261 ttcaatttgt tacagatcag caagaagctc attatgaaga gaaaatgatg ccgacagatg
     7321 aagttgaaac gacaggtgct ccagaacagc cacaagatga gctgtttgaa gaagcaaaag
     7381 cacttgtcgt tgaaatgcaa acggcaagta tttcccttct ccaaagaaga tttcggatcg
     7441 gttacaaccg agcagcacgt ttagtagatg aactggaagc tcatggagtt gttggcccgt
     7501 cagaaggaag caagcctcgg aaagtattca ttgagcagga agaaacatca gaaacgttgc
     7561 cggacgataa tgacatgtaa aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa
     7621 aaagacaagg catgatgata ccaaaagaaa aaatggtttt tttccatctt ttctaatacg
     7681 gtatgatgtc ttgtcttttt agtttttgtg agattaaagt tctattcgat ttatttgtga
     7741 agtattgcat taaggaaatg aatcatgtat agtatgtagc gaaactttga gaaaaagagg
     7801 gaaaatactt tggaaaaaca gtccatttat ggcttgacga atgaagaatt aatcaattgg
     7861 tttatagaaa acggcgagaa aaaattccga gctgcccaag tttgggaatg gttgtatcaa
     7921 aaacgtgtaa gtaatttcac agaaatgacg aatctatcga aacaattgat cgaaaaatta
     7981 tcggagcatt ttatcatcaa tcctctaaaa cagatggtcg tccaagaagc ttcggatggt
     8041 acagtaaaat atttgtttga actccctgat aaaaacatga ttgagactgt actgatgaga
     8101 caagaatatg gcttgtcagt ttgtgtgacg acacaagtag gatgtaatat cggctgtacg
     8161 ttttgtgcaa gcgggctgct caaaaagaac cgtgatctga ctgctggaga aattgttgca
     8221 cagatcatga tggtccaaca ttattttgat gagcgcaatc ttggcgaacg cgtttcacat
     8281 gtcgttgtaa tggggatcgg tgagccattt gataattacg acaatgtaat ggactttttg
     8341 catatcatca atgatgccaa aggattggca attggtgctc ggcacattac agtttccact
     8401 agtggattag cacacaaaat caaggaattc gctaataacg gcttgcaagt caatctggct
     8461 atttccttgc acgcgccgaa taatgaggtg cgaacatcga tcatgcggat caaccgaagt
     8521 ttcccaattg agaaattgat ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga
     8581 atcacgtttg aatatatcat gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa
     8641 ttagcagatc tattaaaaga taagaaaaaa ttagcatatg tcaacttgat tccatataat
     8701 ccagtcagtg aacatgatca atattctcgc agtccaaaag ctgatgtatt gagattttat
     8761 gatgtattga agaaaaacgg cgtaaactgt gtgatacgta aagaacatgg tacagatatt
     8821 gatgcagctt gcggacaatt gcgaagcaag caaatgaaaa aaacagttgc caaataatcg
     8881 tatcctactc gttttttcaa ttaccataaa aggagcagtt ttcttaatta aggaactgtt
     8941 caacttaaat aagagaaaag attctatagc gaaagctata aagctttttt tgacaagaaa
     9001 aaccctcatc aagcaaagaa atgtcctcaa tttgatgaca gcctgcttga tgggggtttt
     9061 agcaattatt taactgattt tcttatatcc ttgctggctt tcttgtgtag catgataagt
     9121 ttctacgtca agaatggcct gtttaaattt ttctcgggtg atttcataag gcacatgttt
     9181 aatatcattt gttgccatgg ctttatctaa gattgcttct aaatcggtcg tgacattgag
     9241 attcatgtcg gttaacgata ctggaagttg gttctttttc ataaacacat agattttatc
     9301 aaacaaatcg aactgctgat ccatcatcaa taaggtcagt acaccataac tgacagatgc
     9361 tccgtgaagg tatccctcca ttttttctag aacagtcgta ccgtaataga atgagtgtgc
     9421 catagcagaa ttatagttat tgttaactaa aacagaaacc aatccggtag taacaatgat
     9481 atccgaaacg acttgttcaa ttgcatatga gggacgatcg tcggcactat ctttcaatgc
     9541 ttgcgtacca taagtcaaga gacgatcaat gcacattttt ccgatttgga ttcctaatct
     9601 atccgtgtag cctagatcat cgccacgagc agaaaaagta gattcgactt ctttggataa
     9661 agcatcgcca atacctgccc ataagtattt atcaggtgct tcggcaataa ttgctgtatc
     9721 aatgaaggtg tgtacaggtg gttttgaagg aaagaagagt ccgtccatta cgccttcttc
     9781 gttatagatg acacataccg cagtgacggg cgaacagttc gaaccaatag tgggaaaggt
     9841 gaagactggt ttagctagtt tttcaccaat gacttttaca gtatcgcaag cacggccacc
     9901 accgactgca aacaggacgt ctgctttttg gacactttca tgctggatca actgttcaat
     9961 atttccataa gttgaattac ccccgtacca gagactggcg acaacctcta tttcctgtgt
    10021 caaagctact tttattttgt tttctgcctt tgaaagtgca gtcttccctc caatcagcac
    10081 tacttttgtc ccatattctt tgacgattcc atttgctttt tcatacgcat ctgttccaat
    10141 ggtataattt ggtaagaaaa cactttgact catattcatt tccttctttc ttcgttgaaa
    10201 cgggttaaaa ttttaaagag tgatcgatct gactgcggct gtagatggag tagaggtgaa
    10261 cctaagacag gataagtgat gaataaggtg gcatgaacac cttgaaatca tcaataagta
    10321 tcctaaaaac gaacaagtac aaaaacattg aaaacgacaa taatgaattt ttgttcctct
    10381 tagtatttct accgattccc tttgcttttt tgtaattatc taaaaattct aaaaattaag
    10441 attattatat gaaaacaggt tttatttgtc aattcataat tggttttttt taaagcaaaa
    10501 gataatctat atcatgatga acaaacagag ttttttcctc caattgatgg ctttcctgat
    10561 ctttcctatt ttgaataaag tgtttctgta taattttttt attttataag taagataaat
    10621 aatgaaagaa tagagatact gattggtagc taaagatttt tgagcgagaa catttcgttt
    10681 tttcaaaagg actgttagaa taagcaagaa caagggggaa gacgtatgat aatcagtgaa
    10741 tttgaccgta ataatccagt attgaaagat cagctttctg atttactgag actgacttgg
    10801 ccggaagaat atggagacag ctcggcagaa gaagtagaag aaatgatgaa tccagaacga
    10861 atcgcggtag cagcggtaga ccaagatgag ttagtaggat ttattggtgc aatccctcaa
    10921 tacggtatca caggttggga attgcatcca ttagttgtag aaagctcccg acgaaagaac
    10981 caaataggta ctcgattagt caattactta gaaaaagaag tagcttccag aggaggaatc
    11041 acgatttatt taggtacgga tgatttagac catggaacaa cgttaagtca aacggacctg
    11101 tatgagcata catttgataa agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa
    11161 ttctatgaaa aattaggtta taaaatcgta ggtgtcttac caaatgcaaa tggctgggac
    11221 aaaccggata tttggatggc aaaaacgatt attcctcgac caaattctca ataataaaaa
    11281 gaggttgtga cagaagtaat cagcttcaag aaataagaag gaatttacga aaattacttc
    11341 ttgaattttt gtgaaattcc aacttatttc cgaagaagct acttttggaa caccgtgtac
    11401 cagaggttgt gaaaaagtcg cttagtttcg agtagtaaga aaaattttgt gaaaatggct
    11461 ttctacattt ttgcataaat ttg
//
LOCUS       DACDUU010000086        11474 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.086, whole genome shotgun sequence.
ACCESSION   DACDUU010000086 DACDUU010000000
VERSION     DACDUU010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.086"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            237..1172
                     /locus_tag="IWL30_002449"
     CDS             237..1172
                     /locus_tag="IWL30_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAP9707172.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVAESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            1262..2365
                     /locus_tag="IWL30_002450"
     CDS             1262..2365
                     /locus_tag="IWL30_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP9707173.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            2378..3331
                     /gene="menA"
                     /locus_tag="IWL30_002451"
     CDS             2378..3331
                     /gene="menA"
                     /locus_tag="IWL30_002451"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP9707174.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(4266..5612)
                     /gene="gor"
                     /locus_tag="IWL30_002452"
     CDS             complement(4266..5612)
                     /gene="gor"
                     /locus_tag="IWL30_002452"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAP9707175.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFCGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(5746..6987)
                     /locus_tag="IWL30_002453"
     CDS             complement(5746..6987)
                     /locus_tag="IWL30_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP9707176.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(7005..7928)
                     /locus_tag="IWL30_002454"
     CDS             complement(7005..7928)
                     /locus_tag="IWL30_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP9707177.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(8149..10641)
                     /locus_tag="IWL30_002455"
     CDS             complement(8149..10641)
                     /locus_tag="IWL30_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP9707178.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(10648..11115)
                     /locus_tag="IWL30_002456"
     CDS             complement(10648..11115)
                     /locus_tag="IWL30_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAP9707179.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(11251..11324)
                     /locus_tag="IWL30_002457"
     tRNA            complement(11251..11324)
                     /locus_tag="IWL30_002457"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11288..11290),aa:Arg,seq:acg)
     gene            complement(11382..11455)
                     /locus_tag="IWL30_002458"
     tRNA            complement(11382..11455)
                     /locus_tag="IWL30_002458"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11419..11421),aa:Pro,seq:tgg)
BASE COUNT         3403 a         2341 c         2007 g         3723 t
ORIGIN      
        1 tattacacaa tctccatata ttatatagat gttctgttct tttgtctaac tccttttgga
       61 tttttttcaa attttaaaat aaagcgtttt ttcacgaaat ttgctgaaag aaaacgtttg
      121 aaatattaca tgggttattt atcaaaaata gttgacttat aggctaaaaa gattagaatg
      181 tataatgaat cacaaagaaa aattgtggac tagaaatgga atggtggggt attaaaatga
      241 agaaaaaacg ttttgtaact ggatttgccg tattagcatt ttcagctcta gtgttaggcg
      301 cttgcggcgc agatgacaac ggaagctcaa actcttcttc tgaatcatct acagcacaat
      361 cttcgactgc aaaaagcaca acagaatcat ctgcaaaagt tgtagctggc ggagatttgc
      421 aagacggcac atataaatta gaagaaaaaa attattcaaa tggttaccgt gcagtctttg
      481 aaatggttgt aaaagacggc aaaatcactg aatcaaaata tgataacgtc aatgaaaacg
      541 gcgaatcaaa aacaaaagat gcagaataca acaaaaacat ggaagcaaaa tctggcacaa
      601 acccagaaaa atacattcct gagttgaatg accaattctt aaaagcacaa agcgcaagcg
      661 gtgtggaagt cgtaactgga gctactcact catctgaaag cttccaaaac tacgcacaac
      721 aattgatcca agctgctcaa gctggtaaca cagatacaat cgaaatcgac aatggtgctg
      781 atttgaaaga cggcacttac aagcttgaag aaaagaacta ctctaacggc taccgtgtgc
      841 aattcgaaat gacagttgct ggcggcaaag tagctgaatc aaactttgat tatatcgaca
      901 aagacggtaa atcaaaacaa gatgatacag aatataatga aaatatgaaa gcaaaaagcg
      961 gtacagaacc aaaaacttac atccctactc tgaacgacga attcgtcaaa gcaatgggcg
     1021 aagaagatgg ctcacctgct aatgtagaag ttgttacagg tgctacacac agctcacatt
     1081 cattcatcat gtacgcacaa caattagtga atgcagctga aaaaggcgac actcaaacaa
     1141 tcgaagtaga taatatcgta acagaaaaat aatcacataa tcatactaat aaaagttgga
     1201 gggtgggacg atagccagat taggcagtcg tctcaccttt caaaatgaag agaggaaaaa
     1261 gatgcggaaa aaactactat tttccttaat cacactgggc atctctttta tggtgctaac
     1321 tggctgcagc aactcagcaa ataaaacaac cactgaaagt tcagcagcta ccaagatatt
     1381 gaaagatcca tattctgatg aacagttctt gttagggact tacgttcgta tccgtgtgta
     1441 tgatgaaggc aaagaatcag cattgaaacc tgccttcgat cgagtaaaag aacttggtga
     1501 taaaatcacg atcaatcaaa aaggttctga gatcgatgaa gtgaatgagc aagctgggat
     1561 caaaccagta aaagtttctg atgacgtcta cacgttagtc aagcgggcct acgaatatag
     1621 ccaagattct caaggcggat tcgatatggc gattggtgca atcacacaat tatggcgaat
     1681 cggttttgat gacgccagaa aaccttccca agaagaaatc gatcaggcat tgaaattagt
     1741 ggattatcat aagatcgaac taaatgacaa agaaaaaact gtttatctaa aagaaaaagg
     1801 aatgatcatc gatttaggtg caatcgccaa aggttacatt acggacgaag tagtgaaagt
     1861 cttgaaaaaa caaggagtga ccactgccat cgttgacttg ggcggtaatg tctatgtctt
     1921 aggccacagc ccaagaggcg aaaaccaaga ctggacagtc ggcatccaag atccgaacat
     1981 ggctcgcgga tctgttttag gttctattaa agaacggaac aaaacattag ttacctctgg
     2041 aatttacgag cgttatttag aagtagatgg caaaaaatac catcatttgt ttgatccaaa
     2101 aacgggttat ccgttcgaca atgatatcgc aagtgtcacg atcataacag ataaatcaat
     2161 tgatggtgac gggttgtcta cagcagtctt ttcaatgggt gtgaaaaaag gactggaata
     2221 cgtcgaaagc gaattgaata acgggacgga agcgatcttc gtgacaaaag acgataaagt
     2281 atatgtgacc gatccaatca aagacacctt caaattatcg gaggactccc attacacgat
     2341 gggtgaccgt tctgaactaa aataaggagt acgaaccatg acactaaaaa tctttttaga
     2401 agtagttgaa atacgaacaa aggtggctag tgtatttcct tttgcggtag gggtattatt
     2461 ctctatcgct ttttttcatg agatacattg gctaaacact tttttatttt ttattggaat
     2521 gctagttttt gatttagcga ctaccgctat caataattac atggattatg aaaaggctca
     2581 ttctgaagta tataagtatg aagaaaatgt cattggccgc agaaatctat ctcctgtatt
     2641 gattcgaaac atgattttag ctatgattgc atttgttttt gttatcggcg ttattctaac
     2701 gatcaatacg ggttggcttt tacttttgat gggatttatc tgttgtttta ttggagtatt
     2761 ctatacattc ggaccgattc ctttatctcg aatgccttta ggggaaattt tcagtggggt
     2821 gactatgggc cttggaattt ttgccatggt cgtttatgtc aatacattcg gttcagatgt
     2881 ctatgatctg acagtcaact tttcatctgg acaatttgct ttggtaggaa atctttgggc
     2941 tatagcagca atcgtattag cttccttacc cttggtgttt acgattgcca atatcatgtt
     3001 ggctaataat ttgcgggatc ttgaccgtga tattgaaaat catcgctata cactagtttt
     3061 ttatatcgga cgtcctgcgg gaacagtctt attccagcta ttgatgtatg cttgctattt
     3121 cgtgatttta atggggttgt tgagcggtat ctatgaatgg ccgatcttgg tcaccttctt
     3181 gaccttgcca attgtgaaga agcatttgga tcaattcaag caaacgttac cccagcctat
     3241 cagtttcagc tattccatca aaaatatggt cttattcaat gccagttatg tattggggct
     3301 attactaaca atcattatgc aagccattta acagcatgaa gaggttgtga aaaaactgcc
     3361 ctgcatcaag taataagaac agccaaataa aaatagctcc ttatattttt cgtttggttg
     3421 ggcttattac cgcagatgca agcttttgaa caccgtttat caggaggttg tgaaaaagtc
     3481 gcttagtttc gagtattaag aaaaattttg tgaaaatggc tttccacatt tttgcataaa
     3541 tttggcttaa taccgagaaa ctggcttttg aacaccgttt atcagaggtt gtgaaaaagt
     3601 cgcttagttt cgagtattaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa
     3661 atttggctta ataccgagaa actggctttt gaacaccgtt tatcagaggt tgtaaaatca
     3721 gtgttttgtc ctgagtatta gagaaacaaa tggaaaaata gttcctcata ttttgccgtt
     3781 ttttgagcta ataccgaagg acagctgatt gaacactatt tgttcggaaa aagtgactaa
     3841 ctatgacaca atttttttca cagtcccttt tttcacttga tttgattcgt accagcgaac
     3901 ttgacgagaa ctttcttggt ttggtaacta tatggtattc ttttaacatc ctcgtttgct
     3961 tcttctaatt tatttataca aagtcacact tttttaaaca tctgttcttc gatacatttc
     4021 tatttctttt aaacatcgat accaatattt tgaacttaag agtgcatacc aaatggattc
     4081 atttactctt cgtcagttac gaagataatc aaaatctcca acttccctac ttatttcgtt
     4141 ttgagaatta tcgaagcaat cttacataaa taactaaaaa acaaaaaacc agaaaatcag
     4201 tatactgatt ttctggtttt acatggtttg ttcaataaaa agctgttgga actgttaata
     4261 ttaaattatc gcatcgttac gaattcttcc gctcctgttg gatggatggc tactgtattg
     4321 tcaaagtctt ctttagttgc acccatcttg atagccacgg caaaaccttg cagcatctca
     4381 tcgacaccaa caccaatagc atgtaagccg acgattcttt cttcttttcc gacacagatc
     4441 aatttcattt cgcatttttg gcgataatca tttaatgcaa agtacattgg ggtaaagcgt
     4501 gaacgataaa ctttcaattc attttcgcca tatttttcca atgcttcttc ttctgtcaaa
     4561 ccaattgttg caattggtgg atgcgtaaag acgactgtcg gaacaagttt atagtcaaga
     4621 taagaatctt tctttccatt aaataaacgt tctgataaac gtctaccagc tgcgattgca
     4681 acaggagtca gatctaattt accgatcaca tcaccaacag caaaaatatg ttcatccgtt
     4741 gtattttggt atttatctac tttgataaac ccttttttgt ccaattccac atttgtattt
     4801 tccagaccta atttttcggt attcggtaca cgtccaccgg caaaaagtac acaatcacca
     4861 gttaaggttt caccattttc aaatagcacc gtatattcgt cattgttttt agtaatttct
     4921 ttagcaatat gatgtgcgta agtttttatt ccagcttctt ggtacatttg gatcaaattg
     4981 tccgaaagca ttttatcaaa cgttctaagt ggtcgttctt ttcgatatgc ccacatgact
     5041 tcactaccta aaccacaaaa tacacctgaa agttcagccg caatgtatcc agcacctaaa
     5101 acgattgctc tcttaggtaa ctctgttaat tcaaaaaatc catttgaatc aattgcgtat
     5161 tctccaccag gaatggtcat tttacttgca cggcctccag tagcgatcaa gattttagga
     5221 gcacgatagg tttcaccgtt tacttcgatc gtttgactat cgataaaagt agcgtaacct
     5281 tttatagcct cgacatgatt gctatctaat cctctttgat aagcaccatg tagaaaatca
     5341 atatattttt ctcgtctttc tactagaccg gcgaaatcaa cattttttaa atctgcttgg
     5401 ataccgtaac ttgaggcatc tcgttcaatt gtttccaaaa tcgtacttgc ttgccacata
     5461 acttttttag gtacacaacc gacgttgaca catgttccgc ccaattcatt cgcttcaatc
     5521 agcaaaactt ttgcgccatg catccctgct ctgtttgctg aagcaatacc gccagagccg
     5581 ccaccaatta cgatgtaatc atatatttgc attttgttcc tccttattga agagatcaat
     5641 caaaaccttt atttaacgat cctttttcta cagaaaacaa gtgtaacact tttcttgaat
     5701 tttgtatgcg aagatgctta aaacagggat atatcagctt tttccttatt tacgttttcg
     5761 gatcatatct ccaggtttca ccggctgtgc gactggcata gttaaaatca tcattggatt
     5821 tggtgctcgg tcaatggatt cgccttcttc attatgcata acttcaacgg tttgactgaa
     5881 atgactgaat cctggaccat aaaattcgat ttcatctcct acagaaaaca agtttctctg
     5941 acgtatcgta gcaatctgtg ttgcctcatc ataagataat acttcaccga caaatttata
     6001 tactgggatt ttacgacgtt caccgaataa ttgttcgttt tcagttggtg tattgtagta
     6061 aaaacctgta gctaattctc gttgtgctac tttccataac tcgtcgatcc attcttgctt
     6121 gcagacatag ttttcaggat cttccatata actgtctaca gccgctttat agacatttga
     6181 caccgtagat acataatgga tcgatttcat tcttccttcg atcttgaagc tgtctacgcc
     6241 gttttcaatc aattctggaa tatgttcgat cattgacata tccactgcac tcatcgagaa
     6301 ttcttcttcc acttcgcctg tatcagttaa actgtttcgt tcttgaccaa aaggcatatc
     6361 gaacaatcca tacttccagc gacaagattg agaacatccg ccacggtttg catcccgcat
     6421 cgacatatgg ttggacaatg tgcatcgacc agaataagag atacacatgg caccatggat
     6481 aaatgcttcg atttcggtat ccgtattttt acgaatctct gccacttcct ccattgaaac
     6541 ttcacgagct aaaacgacac gttccaaccc ttcttctttc cagaattcca atgtctcaaa
     6601 attagttgct gaagcttgag tggataaatg gatcggcaga cctggggctt ctgtcgcaca
     6661 aatttcgatc aaagctggat cagaaacaat cactgcagaa ataccgacat cacgcaaact
     6721 gcggaaaaat tcccctgctc catcttcatt tccttcatgg gtgaccatat tagcagcaac
     6781 gtataccttc gcatcatgtt cttttgcaaa agcaactcct tcagccattt cttcataagt
     6841 aaaattacct gcacggcttc ttaaaccata tgcattcccg ccaatatata ctgcatctgc
     6901 tccataatgg atcgcggttt ttaacttttc cagtgttcct gctggcgcta ggatttctgg
     6961 tcgtttcaat tttcttttta ctgtcatttc tttcatcata ctccttattt gatagcagaa
     7021 ggatcaatat aaaagaatcc tgtatctaat tcacggtttt caggatgcaa tttactgatt
     7081 tcgctagtca ttttttccgc tttttcttga tcccagctac cttctaaaat ttgttttttt
     7141 gcttcggcga atagtcgagt gatgtttacg aatttttcgc ctggagtaaa taatccgtcc
     7201 aatttccatg tacagtaatg atgctgataa agttcattca attcattcga gagattcaga
     7261 tcattgctgg caaaaatatg tgtgccatga ctatcttcat aaatagaata atgtgtctct
     7321 tcttttttag gttccgagat aaaaagacct ctttcacgat ctttttgttc gtcttgtttc
     7381 gtatagttat agtaattctg tagcagtgga cgtttggatt gatggatgca tgtagcacca
     7441 tacacaaggg tttctacagg aatatcgagt ttttcttcca tcgccttcat ctcttcaaaa
     7501 ggaacttcac gagcaagtac tgctccaact gcgccttttt tcgcccagaa attgatttga
     7561 cgtgcgctcg ttactaatgt ttcagcatca taaatatacg gtatacgcaa ttcaggattt
     7621 tttttcatcg tataaataat acctgtatca ccaactgtga tcacatctac gtttatttct
     7681 tttaaaaaag ccaaatattc aggtacattt ttcatttttt caggatgaat gatcccattt
     7741 acagcaatat tcgctttttt accatacttg tgtgctaatt ctaccaattg tcgctgttct
     7801 tctcgtgtaa atgaagcagg cagacgcaat ccaaattgct cttcaccgaa atatagtgta
     7861 tctgcacctg cagcaagcaa ctgttctgct tgttcaatag tttctactgt ggttatgagt
     7921 tcgatcataa ttttctcctc ctacaacttc ataatagtag catagctcta aaaactttcc
     7981 aatacttctt ctatttaaag tcggattttt gaaaattatt tcacaaaaaa tcaaaataaa
     8041 gttcgatata ttctgtccaa atataaaaag agattgtgac aaaaatcttg tcacaacctc
     8101 ttaatcaagt cttactatta taaaaattca gctacacagg ccaagtaact atagggaacc
     8161 tactgtttcc tttttaggtc cgcgaacatt caatgtgatc ttaccttttg aagcacctac
     8221 tgttacgtgg tcacctaaac gaatttctcc gccaagcaaa gcctcgctca atcgatcttc
     8281 aatttctttt tgaagtgcgc gacggattgg acgtgcacca tattcaggat caaatccagc
     8341 tttcccgatc acatcgattg cagatggtgt gattttcact tgaatatctt gttctttcaa
     8401 tcgtttgata atcgctttgc tcatgatttg gacgattgta tggatttcat ctttactcaa
     8461 tgaatggaag acgacagtct catcgattcg attcaagaat tcaggtcgga aagctttctt
     8521 taactcttct aagatacgtt tttgcatggc tttgtgatct ttagtaacat cttggacatt
     8581 gaatccgaca tttttttctt ctcgaatttg tgtcgctccg atatttgaag tcataatcat
     8641 gattgtattt ctaaagtcca cttttctacc tttagaatct gttaaatgac catcatccaa
     8701 tacttgcaga agtaaattga aaacgtctgg atgtgctttc tccacttcat ctaagagaat
     8761 cactgaatat ggtttttgac gaattttttc tgtcaactgt ccaccttcat cataccctac
     8821 gtatcctgga ggagacccga ttaatcggct tgtactgtat ttttccataa attctgacat
     8881 atcaacacgg atcagggcat cttcacttcc aaacatgact tctgaaagtg ctttggccaa
     8941 ttctgttttt ccgacaccag ttggtccaag gaacatgaat gaaccaattg gacgatcagg
     9001 atctttcaat ccgcttcgtg cacggcggat cgcacgtgaa actgctttga cagcctcatc
     9061 ttgaccaacg acacgttcat gcagaagtcc ttccagttct aacaagcgtt cgctttcttt
     9121 tttctccaac tgctgtagag ggacaccagt ccactccgat acaactgtag caacatcttc
     9181 ttctgttaca cgatcagaat aaccttgctc ctgttttgct tcaattgcag aaactttttg
     9241 cagcttttta cgtagtgctt tttcttgtct gcgtaattga gcagcatttt caaagtcttg
     9301 tttttggatc gctgcttcct tttcttgtac tagttcatcg atttctagtt tgatcacagt
     9361 tgattttgtt aagtgatcgg tttgatccaa acggactttc gctgctgatt catcgatcaa
     9421 atcaattgct ttatcgggta gttgacgaga agtgatataa cgaacagaaa gattgactgc
     9481 tgctcgtaca gcttcatctg tgatctccac tccatggtgc tcttcatatc ttgtacgtaa
     9541 tccttgaaga atcacttctg cttcttcagg agttggctca tctacttgaa tgcgagcaaa
     9601 acgtcgctct aatgcagaat ctttttcgat atatttttga tattcatcta gggtcgttgc
     9661 tccgatcgtt tgtaattctc ctcgagctaa cgccggtttc aagatattcg aagcatcaat
     9721 tgctccttct gctccacctg caccaattaa ggtatgcaat tcatcaatga ataagattac
     9781 ttgaccatca ttatagattt cgtcgatgac ctttttcatt cgatcttcaa attcaccgcg
     9841 atatttcgta ccagcaacca aagatcccat atcaagcatc atcaggcgct tttcttgcat
     9901 gtcttctggc acttcaccta aaatgatttt ttgagctaac ccttcagcaa tggctgtttt
     9961 gcctacacca ggttcaccca ccagtacagg gttattcttt gtccgtcggc taagaatctg
    10021 gataagccgt ttcacttctc tcgaacgacc aacaactggg tccagcttgt tttcttttgc
    10081 taatttcgtc aaatcacgtg ctaatgaatc tagcgtaggt gttccttgcg gtgtttgccg
    10141 tttatcttgt acatttacat ttctacgacg attcattcct tttccagcag aatcaccagc
    10201 tagccccatt ttttgtttca gaacttgacg ggcttttgaa aggctgatac ctaagttgat
    10261 catgattcga gtagccatga tactttcatc ttttaataag ctcatcaaaa tatgttctgt
    10321 tccaactttt tgagaaccag ttttcttcac ttcgctgcta gctaaagcaa aaacttgttt
    10381 cattcttggt gaatatggaa gaaagactcc tttagggtaa gaacggatac caccataacc
    10441 aatcagatgt tcaatttctt cataaatttc cgtttcattc aaatccattt cacgtaatac
    10501 ttttccagca attccatttt gctcgaccac taaggctagt aaaacatgtt ctgaactaac
    10561 tgattgatgt ttcaaccgtt ttgcttcttc ttgtgcaatt tctaatactg cttgggctct
    10621 ttcagtaaaa agttcttcca tctaatctca cttcccatct tcataactta aacgttccag
    10681 aaatgctcgg agaatccgag cacgtgtatg atcttcatat tcattgaaat tcaatgtgtt
    10741 ttttgcaatt gcactcaaca tcaagtttcc ttcttttttt gtaattattt ggtcttcata
    10801 aagtttttga ataatagcaa aagcattttt ctcactgatt gtatcatcaa ataactgatt
    10861 tatctgctca agtaattgtt ttttatctga aatccttact tttgcaattc gtatataacc
    10921 accaccacca cgtttgcttt ctactagata accacgttgg atggtaaaac gggtattaat
    10981 cacatagttg atttgtgaag gaacacagtt aaagaggtct gccatttcaa tccttcgaat
    11041 ctcgatcatt tcactatttt ccagaatttt ctttatatat gattcaatta aatccgaggt
    11101 attctgatgt gccatcttat tcattccctt tgactaatat tgaccttaat tataacgaat
    11161 ttgataatag aaagaaagaa gaacgctttt tgttaaataa atcctttaga atcaacaaaa
    11221 aaaaatacgg aatatactat tccgtatcga cgcgcccaag aggagtcgaa cccctaacct
    11281 tttgatccgt agtcaaacac tctatccagt tgagctatgg gcgcatattc aatttaaaaa
    11341 aattataaat catttgaaaa caaaaagcaa gataaaaaaa atcgggaaga caggattcga
    11401 acctgcgacc ccttggtccc aaaccaagtg ctctaccaag ctgagctact tcccgtttaa
    11461 aaaataaaaa agac
//
LOCUS       DACDUU010000087        11461 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.087, whole genome shotgun sequence.
ACCESSION   DACDUU010000087 DACDUU010000000
VERSION     DACDUU010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.087"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(40..2115)
                     /locus_tag="IWL30_002459"
     CDS             complement(40..2115)
                     /locus_tag="IWL30_002459"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAP9707180.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRVSRGHRFLGEDVTFTHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQITAMALSL
                     ASLDQIITK"
     gene            complement(2117..3034)
                     /gene="glyQ"
                     /locus_tag="IWL30_002460"
     CDS             complement(2117..3034)
                     /gene="glyQ"
                     /locus_tag="IWL30_002460"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAP9707181.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3418..4230)
                     /gene="recO"
                     /locus_tag="IWL30_002461"
     CDS             complement(3418..4230)
                     /gene="recO"
                     /locus_tag="IWL30_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAP9707182.1"
                     /translation="MSQLVESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4373..5272)
                     /locus_tag="IWL30_002462"
     CDS             complement(4373..5272)
                     /locus_tag="IWL30_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAP9707183.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5287..5688)
                     /locus_tag="IWL30_002463"
     CDS             complement(5287..5688)
                     /locus_tag="IWL30_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAP9707184.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5666..6142)
                     /gene="ybeY"
                     /locus_tag="IWL30_002464"
     CDS             complement(5666..6142)
                     /gene="ybeY"
                     /locus_tag="IWL30_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAP9707185.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6159..8351)
                     /locus_tag="IWL30_002465"
     CDS             complement(6159..8351)
                     /locus_tag="IWL30_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAP9707186.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEVQEE"
     gene            complement(8375..9346)
                     /locus_tag="IWL30_002466"
     CDS             complement(8375..9346)
                     /locus_tag="IWL30_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAP9707187.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10052..11431
                     /gene="brnQ"
                     /locus_tag="IWL30_002467"
     CDS             10052..11431
                     /gene="brnQ"
                     /locus_tag="IWL30_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP9707188.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
BASE COUNT         3078 a         2416 c         2113 g         3854 t
ORIGIN      
        1 gcacgtttcg tgcatgctct ctgtttttca aacaaaagac tattttgtga tgatttgatc
       61 caagcttgct aatgacaaag ccatggcagt tatttgacta agttgtgtta gtcggttctt
      121 acgtactgct ggttcatcag ccatgaccat cgtttgatca aaataatttt cgatcagtgg
      181 acgaagagcg acaagctggt cgtagttttc gtggatcgtt tggtcagcaa aacttgcttt
      241 cattgcttct actgctttgt gtaattcttt ctctgcgtcg ttttcgaaca gctcagggtc
      301 tacttctgat tgtttttcaa gctgcgcttt tttcgctaag ttcacgacac gtgtcaatgc
      361 ttccattgaa ggtttgaagt cttcatctgc aagatgttgt ttgaacaatt gtgctgctgc
      421 aaatactttt gtcagatctt tttgttctgc attcaaaaca gcttcgatga catcatgacg
      481 gatattcttc gttgtcaata attggcgcag acggcctttg ataaagtcga tcacctgttg
      541 ttgtcccgct gacaagccaa tgccaaaacg atccgtatct ttactgataa cttcactaat
      601 cgtttcttgc agaacagaca aaggaaaggc ccattttttc gattcgatga ttcgaacgac
      661 accatatgct tgacggcgca aagcatatgg atcgtttgag ccacttggga tcatcccaac
      721 tgcaaagaaa gaaaagatac tatctaattt atcagcaatc gctaaaacag aacctactgt
      781 agtctcaggt aaatctcctt cactggatat aggcatgtaa tgttcacgga tcgctgttgc
      841 tacggctggt ttctcgcctt taagcagagc atatttttct cccatgatcc cttgtagttc
      901 agggaattct cctaccatgt tggttactag atcaaattta tagatttcag cagcgcgttg
      961 cagatccatc aattcttcct cagatagtcc tacttgttcg cctatgatgc gagcgatgac
     1021 ttgtacccgt tgcattttct catagatgct accgattttt tcatggaaag taacagtttt
     1081 taatttttca acgtattggt cgattgaagc tttcttgtct tcattataaa agaattccgc
     1141 atcttctaaa cgagcagtca atactttttg attgcctcgt accacatttt ccaattttac
     1201 ccgatcgcca ttacgagcag aaataaaatg aggaagcaac ataccttgat cgttccgtac
     1261 ttcgaaataa cgttgatgtt ctttcataga agtgactagt acttcttcag gaacagatag
     1321 atatttttct tcgaagtcgc cagaaaagac agttggatat tcgactagat tgttgacttc
     1381 ttctagtaaa tcttcatcta gatcaatgat ccagtgattt ttttctgcta attcgtttgc
     1441 ttgtgtgatg atcatttctt ttcttttttg cggtgaagca ataacaaatt gttcattcaa
     1501 tttttcttcg tattcttttg catgcgtaaa tgtcacatct tcgcctaaga aacgatgacc
     1561 tcttgaaact ctgtctgttt tgacatccaa gatctcaaac gggattattt cgtcatctaa
     1621 caatgccacg atccagtgaa tagggcgaat atattcaaaa tcgtattctg cccagtgcat
     1681 cgttactggg aaagtgaggc ttttaatgac atctgctaaa cctgtcaata cttcttttgc
     1741 ttctttgccg tgagtgtatt tcgttacata gacatattcc acgcctttta attctctaaa
     1801 agtgatagca tctgtcgtca gaccttgtcc gcggacaaat ccttcagctg ctttcgtcca
     1861 gtttccatct gcgtccaaag caattttttt ggctggtcct ttgacttctt cttcgctgtc
     1921 tgcttgacgt tctggaatgt ttgttacttt gattgctaag cggcgcggtg tagaaaacgt
     1981 ttcgatcgta tcataagtca attggttttc atctaaaaat tttgctgttt tttcttctaa
     2041 ttgtttcatg cttggtgtga ctacatgagc aggcatttct tccagcccga tttcaagtaa
     2101 taagtctttt gccatcttat tcagcctcct tcacgttttc tggatttgct gtgtttttat
     2161 tcaataaagg gaatcctaat ttttcgcgtt ctgccacaaa gattttggct acagcacgag
     2221 ccatgttgcg gatacgggaa agataacctg cacgctctgt tacggatact gcaccgcgag
     2281 catctaataa attgaatgta tggctacatt tcaaaatata atcatacgct ggatgaacaa
     2341 gattttcatc gatacagcgt ttagcttctt tttcaaaacg atcaaaattg cttagtagca
     2401 ggtcttggtc gcttacttca aaagaatatt tagaatgttc atattctggt tgtttgaaga
     2461 tctcaccata tttcacgcca tcagcccatt ccagatcata aacactttca acttcttgga
     2521 tataagaagc aagacgttct agtccataag tgatctcaga agtaactggt ttacaaggta
     2581 gtcctccgac ttgctggaag tacgtgaatt gtgtgatctc catcccatca agccatactt
     2641 cccagcccac accggcacaa cccatcgatg ggttctccca gttgtcttca acaaagcgaa
     2701 tatcgtgttc taatggatca attcctaaaa gacgcaagct ttctaaataa agctcttgga
     2761 tattttcagg tgaaggtttc attacgactt gaaattgatg atgctgatac aaacggtttg
     2821 ggttttctcc ataacgcccg tcagctggtc ggcgggatgg ctctacataa gcagcattcc
     2881 atggttccgg tccgattgcc cgtaagaatg tatatgggct catcgtacct gctccttttt
     2941 ctgtatcata agcttgcatc agcatgcagc cgtttgatga ccaaaatttt tgtaatgtta
     3001 aaatcatatc ttggacggtc aattttttac tcatctgttt tgcccctttc aatttttaag
     3061 cgttgctgtc aataagaata caggaaagaa aacgacaaaa agtccctatg tctgattaca
     3121 gacataggga cgaataattt cgcggttcca ccctacttcc gaataaagat tcggcagctt
     3181 catttatcgt actcgcaagt gcctcttcaa aaatcgtttg ggtccggctt ccactctccc
     3241 gaactcgcta aaccacgtgt tttttactga tcttggtcaa tgtacgtatt cattttgcag
     3301 ttttctggtt tgtataatag atcctctcag tgaacgatct atttaatcta cggttaataa
     3361 tagacggaag tccctgtatt tgtcaaatag ttttgatttt tttgttgtat ctttcattta
     3421 ttcttctttt tcaggtatct ctttttcagg catatttttc tctggcgtct ttaatacgtg
     3481 tgcccaagac ttcatttgat caatgaactt tttgcttttt agatgaatac cgacatattc
     3541 atcatacaac tggtcaatga ggtcacggat tgcctgcttc gtttcttctt tcaagtggat
     3601 tccactgatc ttatcataag aaatagccga aaacattcga acgaaataaa cagcgcgagg
     3661 atctgcatga taacggtgtt cgtccattgg ccagtgtttc tcgcatatca caccgccata
     3721 tttggaagaa tagtcaaatt ttccttttgt ttccccgcat acgacacagt gcgtccaaat
     3781 tggcgcgatt ccgaagcgtt gcagtatttg cacttcaaag atattcgtga tgatttctgc
     3841 atcatctcct tggtccatcc gctgcagagc ttgttgcgta aattggaata aatgcgggtc
     3901 atattgatga tcttcgattg ctgcatctac tagatttagc aaatacgtac cataaccgct
     3961 caaaaagata tcttcttgga tgttccgaaa cgcatggacg tctttgacac tgcttaaaaa
     4021 cgacaaacct tcacttcgaa aattaccgat atagactgct tcggtaaagg ggagcaaagc
     4081 cggagctaat ggctgatttt gccgatggat tcctttgata taaaacatca ttttgccaga
     4141 agattccgta aagattttga ctaatttatc tttttctttg taattacggc tagataaaat
     4201 caagcctttc gattctacaa gctggctcat attctccctc ctaaaaaata aatctggaaa
     4261 agcagattct gccgatccag attatttatg tttcgatctt ccgctgtttt tctcaacgta
     4321 cggattttta tccagtttat ttctatacct aacctataag aagtccagaa tatcagtact
     4381 cgtcttttcg gtagccgaag tcttgcagat agactttttt gtctcgccag tctttttgga
     4441 ctttgaccca aagttctaga aatactttgt ctcctaaaag attttcgata tcctttcttg
     4501 ctttcgttcc tacttgcttc agcatttttc cgcctttgcc gataatgatt cctttttggc
     4561 tatctctttc gacgatgatc gtggcctgga tatggatttt atcattctcg tttcgcttca
     4621 tcgaatcaac aacgactgcg acagaatgag gcacttcgtc tcgtgttaaa aacaagactt
     4681 tctcacggat caattcagaa acgatgaagt attcaggatg atcagtcact tgatcatctg
     4741 gaaaatattg tgggccctct ggcatttcac tgactaatac atccattaag cgttcgacat
     4801 tgtttccttc tgtagcagag atcggaatca cttgtgcaaa aggcatctgc gtcgaataat
     4861 cttcgatgat tcccagcaac tcgtctggat ggatcttatc gattttattg ataatcagat
     4921 aaacaggaga atgcacgttt tttagacgtt caataataaa atcgtctccc cggccacgtt
     4981 tttgatcagc actgatcata aataagactg catccacttc tttcaacgca ctgtaagctg
     5041 cttctaccat aaaatctcct agacgatgct ttggtttatg gattcctggt gtatcgataa
     5101 aaatcagctg agcttctggt gtagtatata ctccttggat tttatttcta gttgtctgtg
     5161 ccttgtcact cataatggca attttttgac ccacgattcg gttcaataat gtggattttc
     5221 ctacattcgg gcggccgacg attgcgacaa accctgattt atgttctggc atatacgttc
     5281 tcctttttaa ttaaacaaca aatgatatat tttagggaca aataaaatca acccaatgat
     5341 gacagcaaaa caagcggtca ctaaaactgc tccagctgcc atatctttga tttttttccc
     5401 aatcggatga aaatgaaaat ccgtaaacat gtccactaca ttttcaaaaa cagtattgat
     5461 gatctctact atccaaacga gaaaaacagc taaaagcagc cataaccact ctatccgatc
     5521 aagctggaaa atcacgccca taaataaagc aaggataccg aacgcaacat gtttcctcat
     5581 attgcgttct tcctcaaaaa cggttttgac tccttggaca gcaaattcca cagacatcag
     5641 gaaatgtttg tttttctcta cgtttttatc ttttgagtcc ataggcatcc aagatctctt
     5701 tctgtaaacc aaacatttct ttctcatctt ctggtgtcat atggtcatac ccgttgatat
     5761 gcaaaaatcc atgtaccgct aaaaagccaa gttcccgatc atatgaatgg ccgtaagctt
     5821 ctgcttgttc agcagcacgt tcaatagaga tcatgatgtc tcctagtaca cgaggtaatg
     5881 gattctctcc atcttcaaaa atcactggga tttcgtcttc gccttcttct tccagagcaa
     5941 aactgatgac gtctgtcggt gcatcttttc cacggtaatc acgattgatt acttgaattg
     6001 ccgcattgtc catgaaagta acagacattt ctgtttcctc tggcagcttt aaataagatg
     6061 ctgcaaattg cagcaagtca tcgatttctt tcattttctc ttctgagact tggtttgttt
     6121 catccataaa ggtaatatcc attatacatc gcttctttct attcttcttg tacttccttt
     6181 atcttctcaa cttctgtttt cattttagga tatttgatcc gagaatggaa ggtgctactt
     6241 aaaccgctga ttaatgattt ttccactttt cggatttctt tcaaggtcag tccactatcg
     6301 tctaattgac catcggaaat acgctcttcg ataagattat ggacaaaact ttcgattttt
     6361 tcgctcgtcg gatgatccat tgcccgtact gcagcctcac aactgtcagc aatattgacg
     6421 atccctgctt cacgactttg cggttttggt ccaggataac ggaattgctc ttctgttact
     6481 tccggattgc gttctttcgc tttgatgtag aaataacgca ttagcgtggt tccatgatgc
     6541 tgtttgcaaa tatcgatcac catttgcggc atattataat cttccaatat tttggctcca
     6601 tctgtcacat gtccaaagat aatatgttta ctgtcttctg gcaataagaa attgtgcggg
     6661 ttttccgctc ctgaaggcaa gttttcaacg aaaaagctag catgtttgat cttgccgata
     6721 tcatgataat agcaagctac tcgagttagc aaagaacgtc cgccgatttc tgctacagcg
     6781 ttagcactta gattagctac catcatacta tgatgataag tacctggcgc ttcttccagc
     6841 aactgtttca ataaaggatg atttggattg ctgagttcat ttaatacaat tacactgtca
     6901 tcattgacca tcaattcaat atatgggtgt agaccgatcg tcagcaagaa tgagaagagt
     6961 gcaccagtga acccgcagac gagcattaac caagtcgttg catcaccaaa gctcattcct
     7021 tgatagacga tcaaaatgac atccatcatc acaggaaaaa taatgaccca catggcagca
     7081 gataatcctt gttctgagat ccgtttgcgt ctgacaatcg ttgccatgag cccagagaaa
     7141 acataagaca tcaagataat cgttagaaag ttcgtgccaa tcgaatcata aaaaatgaaa
     7201 agtgcggata ctgcttggaa aataccagcc atgatccctg ctcttcgatt caagaaaaag
     7261 cttaaaacaa gtggtgcaaa agcagcggga taaaacagcg ggatataagt agcttgttcc
     7321 gtttggaata attggaaaaa tttcatcagc actatgctga ctgacatagc tgtcacatag
     7381 aaaagcacgt attttgttct ttctcctgca ttttggaatt gaagtgaatt ataaatcagt
     7441 acgccgattt gaagcaaaac agctaaaacc atggcaatta gcggaaaaat cgaagttgtt
     7501 tgattagtca gtcctagtaa ttccaatttt ttcatagctg aagcatcgat ttgattacct
     7561 tcacgaacaa tgatttctcc ttggaagatc atcactggtt gtacagcttc tcgtgcggat
     7621 tgtttcaatt cttctgtctt tttctcattc aatacgtcat tcactacgat accttgatcc
     7681 actaagtagc gaatcatttg ttgttgtgct ggtgtgacat tcaaatattg gatttgctct
     7741 tctgcttctt gtttaaaagc ttccaattca ctttcacgaa cttgtttgct catctgttcg
     7801 tcaatgagct tcagactctc atccctcaca gtatgaatct cagtttcagt catttcaaat
     7861 atcgtcgaat aaaaattgtt cggtagtttt tgataaaaca caacattttc tgcattcaca
     7921 ttttcaaaat ttttcttcag cgcggcgatc cgctcttctg tagtaggctt tgggatattt
     7981 tcatcttcct ttgccttatc aatcttttcg tcataagatt tgttgatggc atcattcgtc
     8041 ttatcgatca attcaaatag ttgcttgatc cgattgttct ggtcatctgc aagatctttt
     8101 tggtaagtgt attctggagt gactgcttct gctgccagtt ttcttttttg ttcggtttct
     8161 tccgtgttct cgatcgtttt gttcgcacgg atactttctt ctgctacttg gccttctctg
     8221 aaatcattgg ccttttgttt gacactacta aatgtaacgc cgcagataat gataaaaaac
     8281 aagaacagca ggaaaggcaa tgctgccttt ccaagttttt cttgtagttt ctgcagtgtt
     8341 ttattcaaca tgtgcttatc ctcctaagca aagcttattc ttgatggaga tctgcctttt
     8401 cataagcttt gatgatttcg gcaactacag ggtgccgaac aacgtcactt gcttcaaaat
     8461 taacaaaatc gattttcttg atttgcttta atgttcgttc tgcatgaaca agaccacttg
     8521 tcgtccctcg aggcaagtct atctgacttg tatccccatt gacaatcatt tttgattgat
     8581 aaccgagacg ggtcaagaac attttcatct gagcgactgt cgtgttttgt gcttcgtcca
     8641 agataacgaa tgcatcatcc aatgttcgcc cccgcatgta agctaaaggt gcgatttcga
     8701 ttacgcctcg ttccatcaat cggttcgtat gatccaatcc aaaaatttgg tataaagcat
     8761 cataaacagg ccgcagataa ggatccactt tttcttttag atccccggga agaaagccta
     8821 gattttcacc tgcttccaca gcaggccgcg tcaaaatgat tttttgcact tcaccttttt
     8881 tcaaggcagc gattgctaat actactgcaa gaaaagtttt tcctgtcccg gctggtccta
     8941 cgccaaatac gatatcatgt ttggctaccg attcgacata tttcttttgg ccgctgtttt
     9001 ttacccgtat aggttttcca ttacggtctt tgacgatctc ttcctcatac atctcaacaa
     9061 aataatctaa tgtacctttt tgccccattt tcaatgccgt cacaacatcg ggcgtactta
     9121 cgtggattcc tcgtttgatc aattcctgta atgtccgaat gactgaggca gccaaggaaa
     9181 tctgggattg ctcaccaatg atctgaatga tttctccacg tgtatggatg gttgtttccg
     9241 ttgtttcctc aatcagtttt atatgcttgt cgtgtgcacc taaaagcatg ctgacatcat
     9301 cagcagccgt caatctaatc tctaaagaac tagactcttc cgtcaaaaat agacatcccc
     9361 tttttttcga ttaattcttt cctattttac catactcaga agaaactata tagcaaataa
     9421 gaaaaggttc taaaagagtt gcttaaccat aagtaccaag aaacgttaaa aaactagccg
     9481 cttccattct ctaaaggtct cgtttgaata aaaaaagctg atcatgtaag ctatgcataa
     9541 gatagaggac aagtattccg tctaacttag caagtattct ctgggtattt ttcttgtttc
     9601 aaaaaaagaa aacaaaatat ttgaaatatt cttgcatttc aaaaaaaaca ggtgtattat
     9661 aacgtcaaca aataaatata cagcaaagcg tcgaggaaaa cagtaggcta ttcagtgatt
     9721 tattcccagt gagtcatgta agtgagaaat gacaataaat cgaatagacg aacatcattt
     9781 ccgagtcaat gtcgaaaacg tagatcattg tggctgcctc cattatcagg ctagcggata
     9841 actttatcga aaagcgtccg tgaatgaaga agctttttta agcgaaaaaa ggtggtaccg
     9901 ttctagaatc tagagccctt ttcccaagtg gaaaagggct ttttttatat tttaaggtgg
     9961 tggttttcat ggaatctgat attagtggta tttctagttt ctgcaacgtt tattttgtta
    10021 ctcaaaatga taattattgg aggaaaaatt tatggaagaa acaaaaactt attcatggaa
    10081 gcaattattg atggtcagtt cacttatctt cgggatgttc ttcgggtcag gtaatctgat
    10141 ctttcctgta cacttaggtc aaatggctgg atcaaactgg atcagcgctg gtgtaggatt
    10201 tgccatctca ggcgctcttt tcccattatt agctatctta gctgtagtag tgacaaaaag
    10261 tgatggtctg tatgacttag ctaaacctgt cggaaaatgg tatgctgcct tgttcttgat
    10321 ccttgttcat ttaacgatcg gaccattctt tggtacaccg cggacagcag caactgcttt
    10381 tgaaatggct gctaaaccct ttttgcctga aaaatatgat tcattaggta tgtttttatt
    10441 ctctgctctt ttctttttac ttgcttattt ccttactgta catccaacac gtttggtcaa
    10501 atatgtcgga aagtatttaa actccttgtt tcttgcatta ttagctatca tttttgtcgt
    10561 ttctttattc caacccatgg ggaatctaaa ccaatctgct gcagattctt atcagacgaa
    10621 cgctttgaca aatggcgttt tagaaggata caacacggtt gatgcagtag ctttacttgc
    10681 tttaagtgtc acttttgtcc atgcagttcg tgatcttggt tttaaagata aaaaagtcac
    10741 agaattaaca gcaaaagctg gtagcctgag cgttgcgtta gaagtcatga tttattttgg
    10801 tctggtcctt ctcggtgcga tgagtttgaa taagatgtct ttatcagaaa atggcggaac
    10861 tgcactttct cagatcacta atttttattt aggtaacttc ggtgctagct tcttaggggt
    10921 aatggtcaca ttaggtgtct tcactactgc tatgggactc gttgtttctt ttgcacaaga
    10981 tttccataaa ctgttcccta aagtcagtta tatgacatgg ctgcggttaa cgacttttgt
    11041 ctcttttgtc gttgcgaatg ccggattgga taatatcatc caatggtctt taccagtatt
    11101 gatgcttttg tatccattgt cacttgcgtt aatcttactt tccttaacag caaaattctt
    11161 ccaaaagagt ccatttgtct atcaagtaac gatgttcttt tctgccgttc cagccgtatt
    11221 ggatatgttg gcaaattcgc ctgcattagt gagtcagcaa actgtcgtag caagcttact
    11281 aggattttat catcattatg ttccttttgc tgcattaggt cttggctggg tcgtcccaac
    11341 attgcttggt tacgctggca gcttgctctt ttattatgta tatcgcgtat caggatataa
    11401 acaagaagca agtgagttac cagaagaata accaaatcaa acacaattta acataagaga
    11461 t
//
LOCUS       DACDUU010000088        11106 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.088, whole genome shotgun sequence.
ACCESSION   DACDUU010000088 DACDUU010000000
VERSION     DACDUU010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.088"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..390
                     /locus_tag="IWL30_002468"
     CDS             <1..390
                     /locus_tag="IWL30_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9707189.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
     gene            778..2724
                     /locus_tag="IWL30_002469"
     CDS             778..2724
                     /locus_tag="IWL30_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP9707190.1"
                     /translation="MIERHVNLLKLLSRQEDFKPASFFSDKLAVSTKTIYQDIELLED
                     YVVNYQVTIERLPSLGIKLIGDRKNVEHLISSVENYLEKDIYSPEKRRVEIIKEVLFG
                     DKPVSLTHLSQSFMVSKTSLYNDLRIINKIIGSGEVKLQSSAEGISFTGNEQAIQRAI
                     KQLVFYYANDMESKTFFQVLNMLFDSKVISVIHDLLFNDYSELTEKVSDYYVRSLIST
                     LVIQCNRLLKGHHLTVEEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLC
                     RQLFAHRVTNNLKTSNEEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLR
                     LKKGIKIQNPLLENIKSQYSELFSIVWYAMSILEEEFDVTLTDDEVSLILIHFQIAIE
                     DQSKANNIIIICQYGISSAQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISS
                     LSFDAGGIPLVRVSPLVSKTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKF
                     VQSSLVKTGVQVESKEECLDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALP
                     HANPSMVIESNIFIVALKNAINWGDRKVKLVIMVSLAEEDVSEIRGVYEELYQFIGQK
                     EMVDRLLQVHDSEKLLRIFQQKGE"
     gene            2727..3182
                     /locus_tag="IWL30_002470"
     CDS             2727..3182
                     /locus_tag="IWL30_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9707191.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            3196..4524
                     /locus_tag="IWL30_002471"
     CDS             3196..4524
                     /locus_tag="IWL30_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP9707192.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            4558..4842
                     /locus_tag="IWL30_002472"
     CDS             4558..4842
                     /locus_tag="IWL30_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9707193.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            4844..5359
                     /locus_tag="IWL30_002473"
     CDS             4844..5359
                     /locus_tag="IWL30_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAP9707194.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            5375..6037
                     /gene="rpe"
                     /locus_tag="IWL30_002474"
     CDS             5375..6037
                     /gene="rpe"
                     /locus_tag="IWL30_002474"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP9707195.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            6044..6616
                     /locus_tag="IWL30_002475"
     CDS             6044..6616
                     /locus_tag="IWL30_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9707196.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(6803..8323)
                     /locus_tag="IWL30_002476"
     CDS             complement(6803..8323)
                     /locus_tag="IWL30_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP9707197.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
     gene            complement(8566..8751)
                     /locus_tag="IWL30_002477"
     CDS             complement(8566..8751)
                     /locus_tag="IWL30_002477"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HAP9707198.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            complement(8735..8917)
                     /locus_tag="IWL30_002478"
     CDS             complement(8735..8917)
                     /locus_tag="IWL30_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707199.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            9158..9376
                     /locus_tag="IWL30_002479"
                     /pseudo
     CDS             9158..9376
                     /locus_tag="IWL30_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            9871..10470
                     /locus_tag="IWL30_002480"
     CDS             9871..10470
                     /locus_tag="IWL30_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9707200.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            10534..>11106
                     /locus_tag="IWL30_002481"
     CDS             10534..>11106
                     /locus_tag="IWL30_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707201.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIGD"
BASE COUNT         3697 a         1755 c         2057 g         3597 t
ORIGIN      
        1 aaagaccgaa aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa
       61 gaagcgaata ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg
      121 acgcagtcac tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt
      181 cgtagaacca tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac
      241 agccgtagaa aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt
      301 tttgatacat acaatcaaaa atttctaaac agaagccata aaggttttca acaggtaacc
      361 gatacattag tttcaatgtt tactgagtaa ctaattattt tgcaggagga caatttattt
      421 acacaaaatt attgacgctc ccaattagga ctcaattgtt catagatgag catttgtaat
      481 taagttatat tataataaaa aaatacgaat tttagtgacg ccattatatt gtttttattt
      541 ttcaattgta atatttcata ttacttccat aataaggagt attttctatt ggaatatctt
      601 agctctttta aggatttctc cttttttgtt tctcattttt taagattatt tggatttcct
      661 ttacaacaaa cttccacaag tattattgac atagatgtaa ttgagggaag ggggttccag
      721 tgctagaata aataatgaaa agaggtaaca aatgctagcg ttaagaggtg aacaagtttg
      781 attgaaagac atgtaaattt attaaaatta ttaagtcgac aagaagactt taaacccgct
      841 tcttttttta gtgataaatt ggcagtgtcg accaagacca tttatcaaga tattgaattg
      901 ttggaagatt atgtagttaa ttaccaagta acaattgagc gattaccttc tttgggtatc
      961 aaactcatag gtgataggaa aaacgttgaa catttgattt catcggtaga aaattatctt
     1021 gaaaaagata tttattctcc agaaaaaaga cgtgtcgaga tcattaaaga agttttgttt
     1081 ggtgataagc ctgtttcttt aactcatttg tctcaaagtt ttatggttag taaaacatcg
     1141 ttatacaatg acctgagaat tatcaataaa attattggca gtggagaagt taagttgcag
     1201 tcatctgcag aaggaattag ttttactggt aatgaacaag caattcaacg agcaatcaag
     1261 caacttgtat tctattatgc gaatgacatg gaatcaaaaa cattttttca agttttaaat
     1321 atgttgtttg attcaaaagt tattagtgtc attcatgatt tgctattcaa tgattacagt
     1381 gaactgactg aaaaggtttc tgattactat gttcggtcat tgatttctac tttagttatc
     1441 caatgtaacc gtttactgaa aggtcatcat ttaactgttg aagaagaatt cctcttcaat
     1501 agcattcgct atatggaaac gtacattgtg gcaaatggtt taattgaaga attgaaggca
     1561 aaattgaatc ttccatttta tgacgcagat aaagaatatc tatgtcgtca actatttgca
     1621 catcgtgtga caaataattt aaaaaccagc aatgaagagt acgctatgag cgttcataaa
     1681 ttgattgaaa gaatgagtga gattgaaaaa attgatttga ctcaaaatga tcgattatat
     1741 caatccttat tgtatcatgt gccagcaatg gtccttcgtt taaaaaaagg tattaaaatt
     1801 caaaatccgt tattagaaaa tatcaaatct caatattcag aactctttag catagtatgg
     1861 tacgccatgt ccatccttga agaagaattt gatgtgactt taacggacga tgaagtttcc
     1921 ttaattttga ttcattttca gattgctatt gaagatcagt ccaaagcaaa taatatcatc
     1981 attatttgcc aatacggtat ttcttctgcg caattaattt acaataaagt tcgacggttt
     2041 ttaccagcaa aagataatgt agaaatatca actttagata agtttcattt agctgaaatt
     2101 ggggaagttg atttgattat ttcctctctt tcctttgatg caggtggtat acccttagta
     2161 agggtatctc cattggtaag taaaacagat tatatgaata ttatggaagc ttatactaaa
     2221 tatttaatag aagaaccttc gcgatttatt caaaatgaac tgattcctaa agcggttaaa
     2281 gttcccacca tatctaaatt tgttcaatca tctttagtaa aaacaggagt acaggtggaa
     2341 agcaaagaag aatgtttgga tttgatgatt tccgaactag aagccaattc attagttact
     2401 gaagagtttc gtgcttctat cttctctaga gagaaaatgg ggaatactaa tttagaaagt
     2461 ggtgttgctt taccgcatgc gaatccatca atggtcattg aatccaacat ttttattgta
     2521 gcattaaaaa atgcgattaa ttggggcgat cgaaaagtca aattagtcat tatggttagt
     2581 ttagctgaag aagatgtatc tgagattaga ggagtatatg aagaacttta ccaatttatt
     2641 gggcaaaaag aaatggtcga tcgcttgtta caagttcatg atagcgaaaa attactaaga
     2701 atttttcaac aaaaaggaga ataaaaatgt atctagataa agaattatgt ttatttcaat
     2761 tagaagtgaa agatcaaaaa gaattgtttc aagtaatgtc tgaacaatta aaaaacgctg
     2821 gttgcgtgaa agatagtttc ttagaaggta tcaccaaccg tgaacaagaa tttccaactg
     2881 gattagaagt aaatcaaatt gggtttgcta tcccacatac agattccgca catgtaaata
     2941 gctctcaaat ctgttttgcc tcattaaagg aaccactggt attttcagat atgacagata
     3001 aatctcatga aatacctgta cgtttagtct ttatgttggc aatgtcacaa cctcatgaac
     3061 aaattgatac actacaaaac ttagttagcc tatttcaaaa tgaagaaaaa gtaaatgaat
     3121 tacttgcatg taccacgaaa gaagcattta ttgagttagt acattctgca ggagtttatt
     3181 aggaggaata gagagatgga tattgtatta aatgcctttg aatggtttat tggattaggg
     3241 tcaaacgtct ttttaccact gattattttt gtaatcggga tattatttgg attaaaacct
     3301 ggtaaagcat ttgtttccgg cgttactgtt ggtatcggtt ctattggttt aggattagtt
     3361 ttaagtttat tatcagatgg tttaggaaca gcaattcaac aaatggggga acaatacggt
     3421 ggtggcctga atatcctaga tattggcgta ggtgttggtg gacctttggc ttactctaca
     3481 tcattgggaa ttttaatgat tccaatttca ttaattctta acttcatatt tgtggttttg
     3541 ggttggacaa aaacattgaa tgttgatatt tggaactttt ggttcgcaat tttcctaggt
     3601 atgattactc aatctgttac gggaaacttt tggtttggct taattggcgc gattgtcgca
     3661 gtaattttac aatggttctt agcagatcgt acacaaaaaa ctgttagcga atttttcggc
     3721 tatccaggaa tctctatcac acatatgatg ggattatcag gagtactatt tgccaaacca
     3781 gtaaattggg tttttgatcg tattcctgga tttaataaaa tagatgcaga tgcagaaaca
     3841 ttaacaaaac gtttcggtgt tttaggtgac acagttgtta ttggcctaat tatcggtatt
     3901 gtagtgggta ttatggcagg ctatgacgta gcaggaattg gaaacttagg aattatgact
     3961 gcggcagtga tgaagattat gcctaaaatg gtagcaatgt ttatggaagg tttaatgcca
     4021 attgcagaag cggcgaaaga atttacgaat aaaaagttaa atggtcgcca agtaaacatt
     4081 ggaatggatg cagcgttaac tgtgggtcat ccaactatta tgtcaacttc tctattgctt
     4141 gtaccaatct cattattgct agcactaatt cttccaggaa ataaagtatt accttttgga
     4201 gatttagcat tctttgcttt cgccatctgt ttaatgattc cttattttaa aggaaatatt
     4261 gtgagatcat tagtaggatg ttcgttgtat gttgttacaa cattatatat ggcaacatgg
     4321 ttggcaccaa ttatcactga tgtgtttaca ttagcaagtt tcgatgtggg gacagcagga
     4381 ttagtaacta tgttgttatg tggattatgg ccagtagggt tgtttgtttt attagcatct
     4441 tactttggtg taattggaat aggtggatta ggagttgttt tagtaggtgc ttctatctac
     4501 atgaacaaag tgaagaaagc ttaatcataa aattaaaata agaaagaaga gatcaatatg
     4561 aaaaaattat tagttatttg tggagcaggt catgctactt ctacaattgc agtagcaaaa
     4621 ttatcaaaat ggttggaaga agaaggatta acagatcaag tgaaaattta tcaatctaaa
     4681 attgctgatg agctaaataa aattgatgaa tatgatgctg tagtaagtac aacaattgtc
     4741 cctgatgtaa ttaaagctaa agtaacgaat ggcttgggct tattaacagg tattggtaca
     4801 gatgccattt atgcagatgt aaaagaaaga ttaggtgttt aatatgcgtt ttcatcatga
     4861 cactttgact attgaaacag tttcagggcg tccttcgtat catgatattc gaaaagaact
     4921 tttagataca gttgatacag caggtattga gaatggaatg ttagtaattt cgagcccgca
     4981 tacaacttgc tcactctttt ttgaagaaac gatgcatgac acaaactttt ttggtgatga
     5041 ttatttacat gtagacatca acaatgtaat ggaaacgatt attccaaaaa tgacgagtga
     5101 aaatcaatac aacagtccgg ggccaaaaca cattgatttt ggaacaagat tatctgatcc
     5161 caattaccct gcagaaaaat gggtcatgct aaataccgat gcacatttac gttcatctat
     5221 ttatggaacc aactcaatga ctttaattgt tcaagaaggt aaacttttat taggagaaat
     5281 gggccgtgtc tattttgtcg attgggatca actaagagag cgaactagaa caatcaatct
     5341 catgattatg ggagaataaa cgggggaata gagaatgaca attgctccat cattgttaaa
     5401 tgcacctatc gcagaagtta aagagttatt tgaaatctta aaagaaaaga atgttgatta
     5461 tttgcatatt gatgtaatgg atggctgttt tgtaccagat atggcttttg gaccaagtgt
     5521 tattaaagat attcgtccac taacagacgt tgtgtttgat gttcatttga tgattgatac
     5581 accagaaaaa attgtcacaa gatttgtaga agcaggagca gatattgtta ccttccacat
     5641 tgaagctacc aaacacgcaa tgaaggtcat tcagttagtc aaggaagcag gagcaaaagt
     5701 agggattact tttaatcctg caacaccagt cagttttatt gaaccagtat tatctttagt
     5761 cgatcaagta ttagtaatga caatcaatcc aggaacagag aataagcatt ttattcaaga
     5821 gacagtagtt aaaatcgaac agttagatgt aatacgcaaa caaaatgatt atacgtatga
     5881 tattgaagta gatggcaaga ttgataatca aacgattaaa gtttgttcta aagcaggggc
     5941 ggatattttt gtatcaggcg gtttcatctt caatgggaaa aataccccgc aagaacaaat
     6001 tgataaacta aatcaatcat tagaggaagt gaagtaaaag atcatgacca ataaagacca
     6061 aaataaaaac tatttagaag cgattatctt agaattacaa aataactcaa aacgtattaa
     6121 ttctgtagag ttagaacaat ttagtgagaa aattgttcaa gctaaacaca tctttctagc
     6181 tggtgcagga cgctctggaa ttgccatgca agcatttacg aatcgcttaa tgcatttagg
     6241 tttttcagtc agccttgtcg gtgaaattag ttcaccacat tcgcaacctg gagatttatt
     6301 aattatctgt tctggctcag gtgaaacagg tagtttaaaa aatttagcgg ccaaagcaaa
     6361 tcaaagtggt gtaagcattg ctttagttac catgaaaaaa gactcgtcga ttggtcaatt
     6421 agcagattct gttcttgtat taccaggtat taccaaagat gaaaatcaac gagaagaaga
     6481 tactttttca caacctatgg gttcaagttt tgaacaatta gcatttctca cctttgatag
     6541 tttagtgtta tctttaatgg ataaaacagg tgaaagctca gaaacgatgt ttgctcgaca
     6601 tgcagatttt gagtagtaga ttttaaagat aaaaaaccat tctctaaatg ctaattattc
     6661 agcaaatatg agaatggttt ttttatttga ttagacataa cttaataatt cagtagtttt
     6721 gagcgtaagc gtgtcataga atggtaccta aacacaaata gcccgatttg atacactcaa
     6781 atcaggttat ttgtggctta tattatttac agatttcttg tatctgctga ttccatggca
     6841 aataagcgcg tagcgcttca ggtgtcatcg aagacagatt gggaatctct gtaaaaagat
     6901 gatttaaata tttgcggatg tttaacccat tcgcaacagc tgtctggata atactcaata
     6961 taattccatt tgatctagcg ccttttaagc ttttagaaaa tagccagttt tttcgaccga
     7021 tcactaactc tttgatggca cgctctgctc ggttatttga aagttccaag cgtccatcaa
     7081 gcaacacaat ctgaagtccg tctttatgat tgactgcgta ttgaaccgcc tttccaagct
     7141 gcgattttgg caaataagca aaactctcac accaatgaaa aaattcagtc atcacaggct
     7201 gcaacgtttc cttcctcgct acaaaccttt cttctgcgct aagcttatta aaggtccgtt
     7261 cgattcgaaa catcttgtca cagaagtcta atcctatttt ggcatgactt tttggggacg
     7321 gttgtttacc caatgcttca aaaaactttc tgcgaacatg cgcccaacaa taaacattgt
     7381 ttaccggcag ctttccatag gccgcgtaac catcagtatg caagaaaccg ctaaatgatt
     7441 ctaaaaaact ttttggctca tcaacggaac gcgaatttgc gtgctcgtag agcacaactt
     7501 gtttttcttc gcttttacct gaagcatata accagtaaaa ggtcttttct ttatgagtat
     7561 tcaatacccg atagcttgtc tcatccgcgt gtaagacctc ctgattgctt aattcttgat
     7621 gaaggcaatt gtagattggc tctaaaacca tttcacttac cttgatatgc caattagtga
     7681 tttttttacg atcaagaggg aaaccaagtg catgccattc tttttcttgt cgatatgccg
     7741 gcaccttttt aacaaatttt tcccaaatac tctgtgtcac aattgaagag gaacctagac
     7801 tattattgat caacggttta ggaacaggcg cttttttgat ttttgtttct cctaatgctt
     7861 cacattctct gcaatgatag gcatgttggt agatgacttg tttttctata tgggcaggaa
     7921 catacaatgg ctcttctcta agccttgtcg caccgatatc atgtaaagta tgaccacagt
     7981 cttcacacaa acaatcggca ccttttaatt cataatgaat aggaacttca ggcagacctt
     8041 ctaatgaaag tgctttgagt cccttgcgtt ttctgcgttt aaaagaagat accgtaatgg
     8101 gttcctggct ttcttgtcca gtgtgctctg gttcattaaa agttgagtcg tctaataaat
     8161 cgatttgccc atctacgggt ggtgtttctt tttttcgtcc aaacaagcgt cgattcattt
     8221 cagcaatttg ttctctaaga agagtatttt cctgttgctg gatttcaagc aactgtgtta
     8281 attgcttgat ttgtacaaga agttcagatt ccatttttgt catttttaca ccaccattct
     8341 agcgattttc ttacatctat tataccattt tatgacaacg aaaaacagac aaaatccttg
     8401 atttaacaag gttttcgtct aatatatcgc accttttttc gtttctttta gcgtacattt
     8461 ttcttcgatt gaaaggccag tcagtaagac ttcttttctg gaacgcggcc attgaaggta
     8521 cccactttca aatcttttgt acattaatac aaatccattt tgatcctagt aaagagcctt
     8581 gaagcgatct ttcttcgtac cgcaaaataa gaaaagcgct tgatcgtaaa catcaaggtc
     8641 atattcttct ataacgaaag ctgctagtcc atcaattcct tttcgaagat ctgtttttcc
     8701 acaaacgata aaaatatgtt tcaatgcagt tgcatcaatg agcattggca ttcaactctt
     8761 ttaacaaggt atacaaaaga tatttatcca ttccattata aataaatatt tccttatctt
     8821 ccgatttgat tctgcaagct agttttggct tggtgtcaat ttgcgatttt tgagccgcac
     8881 ttgaaaattg aactggtaca atatcttttt tattcattcg tttttctcct tctatatttg
     8941 atagtttaat tatagaagaa agataatcgg cgtacgagat actatcctat gacacgctta
     9001 catttaattt aatattttgc ctcaaattag ccacactttt tatagaacta agaggaaaaa
     9061 tgaagttttc ttcctattaa tatagttgtt tgaaatctta aatatagcgt tttacgatgt
     9121 aatgggaagt atggtgaata aatcaactat gttcgtacga agcatttact caactaattt
     9181 aatcgaatct ttcaataaac aaatcaagaa atacagccac cgcaaggaac agttccaaaa
     9241 tgaagagtcg atggaacgtt tcttagtctc gtcttttgat acttacaacc aaaaattcct
     9301 aggtcgcagt cataaaggtt tccaacaggc cgaaggcgag ctcgaacaaa tgctgagtca
     9361 accgatggag aattagaaac aatctacagt agggaagaac cattgacaca aaattattga
     9421 cgctcccaac taaaagtgtc aaaataatga tgaaatcatt tagttcttat aaattagaaa
     9481 agagaaaata aatattccat atctacttta ttggatccgt aacatgacta aattctgaat
     9541 actttttaaa gcttatcatt tggtaaaact gaatataagt taattatata gactgaaaat
     9601 tttgaatttt cagaatagaa taacttaaaa gtactgacaa aattttttaa aggggcaata
     9661 ttaatgttct gagataatca aatcctaccg aaaatatcaa aaatccaatg tgacacaatc
     9721 agattgtgtc tcattcgagg gaatcacaca atcccacgtg gctctaggca gaattgaatt
     9781 tttgcttggg gctttttcct tatattgatt ttgaatgaga cttattatat gttataatgc
     9841 aattaagtat cattggagag ggcgaagaaa atgaagacgg tagaacctat tcgagacaaa
     9901 aaaaagatcg atgcgatgaa ggcaatcttg gcttcgggaa aatatggcca acgaaatttg
     9961 gttctttttt cgattgggat caacaccgcc tatcgcattt ctgatttgcg tcaattgaag
    10021 ctggccgacg tgctggaaat ttcacgcggt cgcgtggtgg tcaaagatcg attagcgatg
    10081 aaggaacaaa aaacagctaa acataattcg gtctatattt ccaacaaatt gcgaaaaacg
    10141 attttggagt acgttcaaca ggaatttcct gatcaactcc ttcagcaaga ttttgacggt
    10201 tatttgttcc ctagtcgaaa agggacgaat gaaccgctca accgccaaag tctgtggcga
    10261 atcattcatg atgctggcac ggcaatcgga ttgaaagaca ttggcccaca ttccatgcga
    10321 aaaacgttcg gttactttct ctataaacag gggaccaaaa cagaaatcat tcagtccttg
    10381 ttgaaccatt cttctcagcg cgaaacattg cggtatattg gtattacaca agaagacaaa
    10441 gatacagctg taaaaagctt agatttataa tataaaagtt aagtttaaac atggatagtt
    10501 ttaaattagt agattgagtg ttgggaggaa agaatgagta cattatatat tataggaaac
    10561 ggttttgatt taagtcatgg tttaaaaact agatacactg atttttcaaa atgggttgaa
    10621 aaaagtcata gtgaaatatt cgataaaata gacacaatga taaatgaaaa taatgaggtg
    10681 agaagtacag ctgatgataa gataggttgg tttaattttg aaagtagttt gcatgagtta
    10741 attcaaagtc cagtctttga agattatgta gaagagtcta aggattatct cataggttat
    10801 ggacatgaag attggtcaga ttctgcccat catgattttc aaaatatggt ttatgattat
    10861 gttgtttctc tagaagaatt acaacaactt ttcaaggaat ggatacgcaa agaaatacag
    10921 ccgacaatag aacataaaat tccaaaatac caattttcgt ccgatgtact attctttaca
    10981 ttcaattata cagatgtttt ggaaattcta tacaagcaac cgaaagaaaa catatatcat
    11041 attcatggca gttatgacga tataataatc ggccactccg agaattttga caactttata
    11101 ggagat
//
LOCUS       DACDUU010000089        10272 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.089, whole genome shotgun sequence.
ACCESSION   DACDUU010000089 DACDUU010000000
VERSION     DACDUU010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.089"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..209
                     /locus_tag="IWL30_002482"
     CDS             <1..209
                     /locus_tag="IWL30_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707202.1"
                     /translation="GQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRMDPNHLKL
                     ENQDRYIDQLVFTFRRLLKSDCHG"
     gene            202..606
                     /locus_tag="IWL30_002483"
     CDS             202..606
                     /locus_tag="IWL30_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707203.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            618..1226
                     /locus_tag="IWL30_002484"
     CDS             618..1226
                     /locus_tag="IWL30_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP9707204.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            1246..1608
                     /locus_tag="IWL30_002485"
     CDS             1246..1608
                     /locus_tag="IWL30_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707205.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            1611..1793
                     /locus_tag="IWL30_002486"
     CDS             1611..1793
                     /locus_tag="IWL30_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707206.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            1810..5241
                     /locus_tag="IWL30_002487"
     CDS             1810..5241
                     /locus_tag="IWL30_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP9707207.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDFIVAIGGLTAAFGILLPAILAVSYLFGPMML
                     IIGGIIAVIAGVIVAIKNWGAITDWFSGLWKKFTDWLGDTWESIKDGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPEFIMEGSF
                     NPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGVGEAGPEAVLPLKRSVLQEIGDRILS
                     STSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
     gene            5292..6029
                     /locus_tag="IWL30_002488"
     CDS             5292..6029
                     /locus_tag="IWL30_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP9707208.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            6039..8330
                     /locus_tag="IWL30_002489"
     CDS             6039..8330
                     /locus_tag="IWL30_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707209.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVDGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            8354..>10272
                     /locus_tag="IWL30_002490"
     CDS             8354..>10272
                     /locus_tag="IWL30_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707210.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSENPNLMPYITEPWVG
                     PLLGNGHTVKDSVKRVITHTKTRTANSGDILSLGLGIPCTAEANNRYLITTLRPSTTY
                     TLSVTMSVGSDWTGETNTIGVRLRYLNEQGGIELPINALIPANVERDKMVTHTFTGIT
                     KDNVTSITNCYVEIFSLNSEYKGTVSVSYDVKLKAHYPNLLDGPYWLGKVPLGENIAD
                     PTVVFPHKTSEYMVYGRRNTENYIADQTYTISMKATKLTVQSFAVYIAAGRVKVGDMK
                     PTEGLANTWELTFTVTKQHIDSGVTNYLEIYQYPSATKGAVQIEWLKLEKGNTRTPNI
                     SEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINS
                     QLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKL
                     YREGKTVSIYFVALNG"
BASE COUNT         3594 a         1573 c         2172 g         2933 t
ORIGIN      
        1 tgggtcaaaa tctatatcta actttctatt cagaaaatag agaagattta acaggagact
       61 cactagatat tatttcattg attcaaaatc gttcgattcg ttttcagaga atggatccca
      121 atcatttaaa actagagaac caagatcgct atatcgatca attggtattt acgtttagac
      181 gattattgaa gagtgattgt catggctaaa aatagttggg agctaaaaat aaatggacat
      241 gatgaacttc ttgtgcggat ggaacgctat tcaagcgaga gcgaacgact gattaacgaa
      301 gcattgaaat caaaaggttc ggctattgca gtggatagga ttacagaaaa aattcctgtt
      361 tctgaagcag atttaagaag agggcaccaa cacgcaaaaa atagtcgtcc acttaagact
      421 caatacatta atttgggttt catcattaga cctacaagaa aatttgagta tttaaaatat
      481 cctgatttgg ggataggtac ttctaaaaga aatcagccgg acgaatttat gagaagagga
      541 ttaggtcttg cacttgatcc aattacagaa cttctgattc gtcaattcga taaattaaat
      601 aaataggggg aacaacaatg gctaaaacaa aaactgtagt aacaacgttc gataacgtaa
      661 gtatcaaacg aattgctttt aattttaaga acgcaggaaa tgcaatcgca acgaattgta
      721 atggacaatt agatggcgaa acagaaatgc aaacggtggt taaaaaatgt ggagcgacag
      781 aagtaaaatc aaaatctaaa ccaatcaata tgacggtaac aattactgca catgtaccga
      841 tggaagttta tcgacgtttc aatgggttga aacaagatgg acgtattaaa ccaggcattt
      901 actcttacgg tcctgattcc gtaggcgaag atttctcact tgctgcagag atcgtggatg
      961 acttcgaaga aaatagaaag ttagttggta tgttagcatg cacttcgaat acaggattaa
     1021 cattctctat tgaaaatggt gcggatgaag tagctgcgtt agaactagaa acaaaagtta
     1081 tgcaagatga atttggtaaa ttttatcatg aagcaattgt tgcagaactt gaagaagact
     1141 taacagatca atggatgacg aatctatctg ctgatgtgat taaaaaaact tcagttgtga
     1201 caactacggc cactcaatca cagtaaaaaa aacggaggta gcgaaatgaa cgaagattac
     1261 tcaaaaattg aactaaacga tggaacaatt ttgaatttag aacctaaact gaatatcaag
     1321 aaattattga tgatcaatag agattttaac acagacgagt ttgcaaaaat gactgtggga
     1381 aaaggatcca tggatatttc tgttattcaa ggtgcaaagg ctgtgtatat tgcttaccgc
     1441 caagcgaaca tgactgatta tatttcattc gatgaattta tcgataaatg ggattttgat
     1501 atggctactg ccagctatat ttatcaattg atgatgttca aacaagcacg cgatgcttat
     1561 caaaaagaat ttgaaaaagc aaataaggaa aaaaagcttc aaaagtaaaa attccaaagc
     1621 tcttagttga aacgtgggtc gatgtctatt cgatgttgac cgacgttttt tctatgcctt
     1681 cagatttggt tttaagcgat atctgtttag atgacatttt gcaaatggct tacaacaaga
     1741 gtgcttatga aggatggaaa aactatgcaa taaaccaatc ccagaaaaac taaagaaagg
     1801 aggtaaaaaa tggctaaaaa gagaacagaa gcagaagtaa ctttcatagc taacgatgac
     1861 ggattgaaat ctacgttaaa agaaatcagt gctgaattaa ctaaaaatag agcagaatta
     1921 aaactagaac aagctcaatt acaacagact ggttctgaat cagacaagtt aggaagtaaa
     1981 ttatcttctt tagaaaagca gtatgaatta caaagtcaaa aagttgaagt aactagccaa
     2041 cgtttagcca atgcgaaaaa atattatgga gaaaattcca ccgaagttca gaaacttgaa
     2101 agagaactga ttaatcaaca aacagcgcaa caacgtttgt caaacgaaat tgataaaacg
     2161 agtaatgcac tagctcaagc aaaaggcgaa atacagacgt acgagtctac aatgcaacag
     2221 ttggatagcg aacaaaaaaa tgttcaagct agtgcttctc tgattgaatc cgaatacaaa
     2281 aaatggcaag caactgctgg tcaatcagct tctgaagccg agaaattagc gaaagcccaa
     2341 gaatatgttt ctcaacaatc tgaaaatgcg gagaaaacga tagatatcct aagacgacag
     2401 ttagaagcta cacagtctga atttggcgct acatccacag aagcaatgca gatggaagcg
     2461 aagcttaatg atgctgaacg tgaatttgaa gagttaggac aagctgctaa aaatgtagat
     2521 acaactaact tggacgatat cggaagcaaa atagatatga ataatttaat ggaagcttct
     2581 gacgttttaa gcgacattgg cgataagctt acagaattag ggaaacaagc agtggactct
     2641 gctaacagtg taggtagttc ccagagtaag atacaagcta attttggttt gtctaaacaa
     2701 gaggctgaag aattaacgaa tgtagccaga gacatttatt ataaaggttt tggagaatcg
     2761 ttagatcagt ccacagatgc attgattttg gtaaagcgta atttaggaga tttaaataat
     2821 caagatttac aaaatatcac ggaacaagct atggtcctag aaaacaccat gggcgctgat
     2881 atggatgaaa cgttacgtgg tgtaaatggc ttaatggtca atttcggctt gagtgctcaa
     2941 gatgcaatgg atttaatggt ttcgggtact caaaacggtt tagataaaac gcacgaatta
     3001 ggcgacaata tggcagaata tagccaatta tggagtcaaa tgggatattc agctgatgaa
     3061 acgttcggaa tgcttcaaaa tggtttagat gcgggtgctt ataaccttga taaagtcaat
     3121 gacttagtta aggaaatggg aatatcgtta acagatggtc gatttgagca aaacatggat
     3181 atgtttagtg aaagtactag aaaagctttt gaagagtgga aaaatggcgg aggaacacaa
     3241 aaagacgtta ttaattccat gattcaagat tttagcaata tggatggtca atacgaccaa
     3301 ttaaataaag cttcgacaat ttggtctgcg cttggcgaag ataatgcgat gaaagttgtc
     3361 caatctttaa ctgatgttaa ccatacattt gatgatgtta gtggatctgc acaaaaaatg
     3421 aatgaagatt ctactactcc gttgcaagag ttgaacggga aaatagctga attaaaggat
     3481 tcattagctc ctataggcaa cacaatcata gatgcactcg aaccagtaat tgattttcta
     3541 ggaaagatgg ctgatgcgtt taataatctt ccacaaccag tacaggattt cattgttgct
     3601 ataggtggtc ttacagcagc atttggcata ctgttaccgg caatactagc cgtgtcatat
     3661 ttatttggtc cgatgatgct tataattgga ggaattatag ctgttatagc aggcgttatt
     3721 gtggcaatta agaactgggg tgcaattact gactggttta gtggtttatg gaaaaaattt
     3781 actgattggt tgggtgatac ttgggaaagt ataaaagacg gagcctcatc agtttgggat
     3841 ggggttaaag aaacctggtc tggatttgta gattgggttc aagatatttg gcaaggagtt
     3901 tctgattggt ttggagagct atggagcgga ttagttgaag gagcttccaa catctggcaa
     3961 ggagtccaag aaacttggca aacattcgtt gattgggttt caaatatttg gaacggagtc
     4021 aaagaagtat ggtcgattat ttgggcagac attgtaggaa ttgttcaaat accatggaca
     4081 ttaataacgt cattgattca agctggtatt aatattatcg tgggtatttt tgatgtagct
     4141 ggacagttat tgggcgcagc ttggcaagct gtttggacac ctatttctga tttccttaaa
     4201 aatacttggg atactatgac acaatggata agtatcgctt ggaatgggat tgtaactaca
     4261 ttccatacta tatttgatcc agtagtggca tggtggaatg gtatatggac agctattagt
     4321 actacggctt caaatatttg gaattcaatt agtgcaacag cttctagtat ttggaacagt
     4381 atcaagaata caatcactag cttggtacaa gcagctgcta cagtaattca aaatatttgg
     4441 tcaactgtat ctagttggtt aggtggaatt tggaattcaa tcagctctac agcatcaaat
     4501 atctggaata gtgtgactag tagtataagc aatgctataa acgcagctaa aagtgccatt
     4561 caaagtgttt ggaatagtat atcttcgtgg attagcggaa tttggaacgg tatcaaaaat
     4621 actgctttga atctttggaa tggaattaca agcactatta gctctaaagt aaacgatgga
     4681 aaaaatgcaa tttcaagcgg ttggtccaat ctaacaggta ttgtttccga catattcaat
     4741 aatgttaaaa gtacaattgc taacatatgg gaaggcatca aaaagactgt tagcgctcca
     4801 attgattgga ttagagacaa aatcagtggc atttttgata atttgaatat ttctatacca
     4861 catattccgt taccggaatt tatcatggaa ggcagcttta acccgctaaa aggtcaaatc
     4921 ccccatttgc gtgtaaaatg gcatgctaaa ggaggtatct ttactaaacc aactttacta
     4981 ggtggaatga acggtgtcgg tgaagcagga cctgaagcag ttttaccttt gaaaagatct
     5041 gttttgcaag aaattggtga tcgtatcttg agtagcacat cagtttcatc tagggcacaa
     5101 acgattcaac ctgtgaataa ctacgaattc aatttcacaa ttgatggtaa cgcagatgag
     5161 gttactatga agcaaacaac tcaacaaatc attgatagca ttacaaaaat tcaaaatgat
     5221 aatgcttcgg catggcgtta aacaggagag tatttctcct gtttttttag tattaaaaag
     5281 gatgtgaaaa aatgactgat tgtatacatt ctataatcga tggatttcct gattatttgc
     5341 ataaattggc tttagctgaa agaccaacca taccttctcc aaaaagacag agagttgaaa
     5401 cttctgtttt aggtaggtta ggtggcttag tacaagatta ctcgtttgaa gatatgtcgt
     5461 ttacattgca ctataactat ttagaggatg tggaagacca tcaagcgttc aagcaatcgt
     5521 tttatatcat gcgtcattgg ttaaactatg caaagaaatt agaattctct gacgatccca
     5581 acgtctatta cgttatccag actatcgata ttggggatgc agaaaacgat attgttgaat
     5641 ggggagagtt cgatgtaaat attactgcga aaccattcgc aagagttcaa gaagatgtac
     5701 ctataaccgt agataaacca cagtcattta acttgctgaa taatagttta gaagaaagtt
     5761 ttccaaagat tatcatcact ccttcagcta cttcatgcca gttcatctta aatgattatg
     5821 tgtttagttt cgaaggctta gtagcaggaa ctgatgtagt cattgatagt gatttgatgc
     5881 tttgctacga agagcaatcg gacggagata ttttagatcg gtccaacaaa atgaagacca
     5941 tgcaatatcc gacattgcaa gtggatatca atcattttaa ttgcactggt ttgagcaaaa
     6001 tacaaattta tcgtaacggg ttaaggtagg tgaaatagat gatcgataat ttaataacta
     6061 tttacgataa aaatgacgcg aataatttag ctgaacattt atatgatacg caaggtttag
     6121 gcgctttgtc agactggtta acagctactg ttagcaataa actaaacgga gccgagatat
     6181 ttcagggtac ttattcaata aacggaacta atgcagattt gattgtagac ggacgtatta
     6241 ttcagtgtta tgtagatgaa aatcgagcaa aacagcgtct acggatttat tatgcaaaaa
     6301 cttctgtaat aggcaatacg atagaagtaa aagctgaacc tattttcaat gatataagaa
     6361 aatcggtgtt gaataaatat gacagcggaa cagaaaagat cactgctact caggcatggc
     6421 aaaacgcaaa agttttagcg aaaccagcta tcccttcgca gttttctttc tcgtcattag
     6481 tagatacgct tgctaatgtg aagatagaaa aggcgaattt tttagaattc tttggtggaa
     6541 aagagggatc tattctagat cgatttcatg gtgagtttct aaaagataat aacacattac
     6601 gtcatgaaaa aagtctaggt acggatcata aaatcaaagc gatttatact aaaaacttaa
     6661 ctggtcttga cttagagatt gatgctcaaa gtgttttagt tggagtttat ccattcatta
     6721 gcagctcttc agaaggagaa gacgagatca ctctaccaga agaagttatt ttcacggatt
     6781 acgtggatga ttatcctgct ggatatgttt cttttgttga ttttaaagac aaagcgactg
     6841 atgtagccac attaagggaa gctgctaaag actggttgaa aacaaacata gataaacaaa
     6901 aaccacaagt gagtggttcg attgaattag taccattgag gcatcaaaga ggctatgaaa
     6961 aatttgttga tctagaaaaa gtttcgatgg gtgacggagt agatgtgtat catccacagt
     7021 taaaagtgaa tatgtcagcg agaattgtgg aatatacgtt taatgttcta accaattcat
     7081 acgataaatt agttgtagga aacgtcaaaa caaacttctt agaaaacaca gagaataatg
     7141 tcagcaattt gattaatgat gccattgatc aattgaaaaa cggtggcgaa atcagtgatt
     7201 tactcaatga tattgtagat catcaaactg atatgattac tggtcaaaat ggtggttatg
     7261 ttttattaga tcctaaagaa gcgcctagtc gtattttgat tatggacaca ccagataaga
     7321 ataccgcaag gaatgtttta caaatcaaca atgctggtat tggtttttct aaaactggca
     7381 ttaatggaac atatgaaacg gcatggacgt tagatggcgg attcaatgcc tcgtttatta
     7441 cggctggtga aatagtagga attactatta gaggtactac attaattagt gatggtgctg
     7501 attatagaac aagtattgct aatggcaaaa tgacttggta ctcaaaaaaa gttaacaaag
     7561 atattatgga gctagaagca cgtgattatg taagtgctga tgccggtatt gtatcataca
     7621 ccatgaaaac tggtggtggt ttcatgatta gaaatccaca gggtaacttg gtttttagta
     7681 cgtgggataa tggtaataac agaccgtttc tatcttttgg tgcgcccaat ttcaggtata
     7741 gcaatgctag ttatgtaact tctggcgacg gtagttcttt aagcattaat ggtagtgcgg
     7801 gtaactcatg ggaatttaag gtagctggta ggactatgaa atttactagt gatggtatgc
     7861 taacgttacc aggttgtttt tttggttcat gggaagatgg gaaacttgct aggtttgaac
     7921 aatcaacggt acaagtatat aaagatttta ctgttagagg tactaaaaac tcaactgtac
     7981 caacagaaca ttatggacaa cgactattga acgcttatga aactccagaa tattatttcg
     8041 ctgattatgg ggaagccgtt acaggtgaca atggtaaagt tcgtgttgat attgacccca
     8101 tgtttgctga aacagtaaat ctaagtcggt atatgacaca tgtgacacct acagaactag
     8161 ttttgtgtgc tgttactcat gaagatattg accatttcat cattgaaact agtaagccaa
     8221 acgtattagt tagatggaat ttagtggcac accgtctagg gtatgaagat attagattaa
     8281 aagaggatac agcatatgat agcacagtgc ttgaccaaaa acgtttttaa aacgaagaca
     8341 aggaggtata taaatggcta gcagtttata taatttggct ttagatttca gcaaagaatt
     8401 aaactacacc aaagctatta tggctcgtca aggtgataaa gggattacgg tgacggttaa
     8461 accgtttcta aatggcttgc agatggatac gagtggcgga acatttactt taaaaggaac
     8521 aacaccatct aaccgttacg tagataatgt tgcaactagt gtaactagtg aagaagtcac
     8581 gttttctctt gatggcacat ttatgagtga agcaggatat tataaacact gctacgtaga
     8641 atatagaaaa gacaatcaaa ttttaacaac gcaagatatc atttttttct cactaggagt
     8701 gtctgacatt tcgcaaggcc aagccgatga atatgtttcg caattagaag agttgattcg
     8761 aaagtacaac gaaacttttg atgcttttat ggctgaaatc aaaggtagag tggatagctt
     8821 aaatcaacag attactgatt taactggtca agctaaaacg ctacaagaca agttagatgc
     8881 tctgaaagaa gaaatttcta agttaggtaa cttacaagtg atgtacagta acagcatcga
     8941 cttcggggac tatgattatt cggagaaccc gaatttaatg ccatatatta cagaaccttg
     9001 ggttggacct ttgttaggta atggacatac agttaaagat agtgttaaaa gggttataac
     9061 tcatactaaa actagaacag ctaattccgg cgacatatta tcgcttggct taggtattcc
     9121 gtgtacagcg gaagctaata ataggtatct tataaccact ttacgaccat ctacgacata
     9181 cacattaagc gttactatga gtgtcggttc agattggact ggagagacca atactatagg
     9241 ggttaggctt agatatttaa atgaacaagg tggtatagaa ctgcccatta atgcattaat
     9301 accagcaaat gtagaacgcg ataaaatggt cactcataca tttactggta taacaaaaga
     9361 taatgtaact agcataacaa actgttatgt tgaaatattc tcacttaata gtgaatataa
     9421 aggaacggtt agtgtcagct atgacgttaa gttaaaagca cactacccta acttactaga
     9481 tggcccttac tggttaggta aagttccttt aggcgagaat attgctgacc ctacagttgt
     9541 ctttccacac aaaactagtg aatatatggt ttatggtaga cgcaatacag aaaactatat
     9601 cgctgaccaa acatatacca tatcaatgaa agcaactaaa ctaacagttc aatcgtttgc
     9661 cgtgtatatt gctgctggcc gcgttaaagt aggagacatg aagcctactg aggggttagc
     9721 taatacatgg gagttaactt ttactgtaac taagcaacat atagatagtg gggtaactaa
     9781 ttatctggaa atataccaat acccgtcagc tacaaaagga gctgttcaaa ttgagtggct
     9841 caaactagaa aaaggcaata ctcgaacccc gaatattagt gagtataaat atcgtggtac
     9901 tggtatgcgt gattcaaaca atccaaaaga ttatgtttgg gatctagcac cagaatatgt
     9961 cgaagataat ttggccacag atattaaaat ttctgaaatt actggtaaag caaacaatta
    10021 taccgatggg aaagtatcgg agattaattc gcagttgact gcttcaatta atgaagtaga
    10081 caccacagct aaggatgctc aaacaaaagc gaatgctaat gcgactgcta tagatgaatt
    10141 agacaataag atcgatgaac gcattaatga tacagctact accacattaa cagttacaaa
    10201 cgggaatacc ggatcagcaa agctttatcg tgaaggaaaa acagtttcta tatattttgt
    10261 ggctttaaac gg
//
LOCUS       DACDUU010000090         9859 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.090, whole genome shotgun sequence.
ACCESSION   DACDUU010000090 DACDUU010000000
VERSION     DACDUU010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.090"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            42..1301
                     /locus_tag="IWL30_002491"
     CDS             42..1301
                     /locus_tag="IWL30_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9707211.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            1311..2183
                     /locus_tag="IWL30_002492"
     CDS             1311..2183
                     /locus_tag="IWL30_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9707212.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            2195..3025
                     /locus_tag="IWL30_002493"
     CDS             2195..3025
                     /locus_tag="IWL30_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9707213.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            3065..5248
                     /locus_tag="IWL30_002494"
     CDS             3065..5248
                     /locus_tag="IWL30_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP9707214.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            5309..6529
                     /locus_tag="IWL30_002495"
     CDS             5309..6529
                     /locus_tag="IWL30_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HAP9707215.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            6526..7986
                     /gene="gtfA"
                     /locus_tag="IWL30_002496"
     CDS             6526..7986
                     /gene="gtfA"
                     /locus_tag="IWL30_002496"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HAP9707216.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            8063..9169
                     /locus_tag="IWL30_002497"
                     /pseudo
     CDS             8063..9169
                     /locus_tag="IWL30_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            9491..9700
                     /locus_tag="IWL30_002498"
     CDS             9491..9700
                     /locus_tag="IWL30_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP9707217.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            9693..9842
                     /locus_tag="IWL30_002499"
     CDS             9693..9842
                     /locus_tag="IWL30_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAP9707218.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
BASE COUNT         3363 a         1506 c         1945 g         3045 t
ORIGIN      
        1 aaaaagaaaa cgcattcaaa ttgtataagg aggagcataa aatgaagtgg tctaaaaaaa
       61 ttattttagc ttgtatagca ctaggagcaa cgatctcttt gactgcttgt ggaaaaaatc
      121 aggaagatgc aagcggtaag gtagaaatag agttttttaa tcaaaaaaaa gaaatgactc
      181 aaacgattca ggagattgct aaagattttg aagcaaaaaa ccctgatatt catgtaaaag
      241 ttgtggatgt accgaatgct ggtgaagtaa ttaaaacacg tatgcttgca ggcgatgtac
      301 cagatgtcat taatctttat cctcaaagta tagaattgaa agaatgggcg aaagctggtt
      361 atttagagga cttaaccgaa gaaccgtatt tagagaatat caaaaatgga tatgcacaac
      421 gttttgctat tgaggatcgt gtatatagca taccattaac cgctaatgtc tatggttttt
      481 actataacaa aactgctttt gaggaaatgg ggattcaagc accagaaact tgggcagaat
      541 ttgaaaaaat agtagctgag attaaagatc aaaacaaagt accgtttgcg attgcaggag
      601 cagaaggctg gactttaaat ggttatcacc aactagctat agcgactgct gctggtggtg
      661 aagaggaagc gaacaatgtt tggcgtttct cagaagttaa tggaattaat gctgaaagca
      721 aagaaatgca aaaagatttt aatcgattag atttgttaag agaaccaaaa gctttgcaaa
      781 acaactggca aggtgccggt tataacgata cagtagttac ttttacaaaa ggtgatgcgt
      841 tgattatgcc gaatggttca tgggcaatgc cgatgattca ttcacaaaat ccagattttg
      901 aagtaggaac ttttcctttt ccagcagatg aagcaggtca aagtctaacg attggcgcag
      961 gagacctagc cttatcagtt tctgcaactt ctcaacataa agaagaagcc aaacgttttg
     1021 tagaatatat gacaacacct gaagcgatgc agaaatatta tgatgtagat ggtgcaccgt
     1081 gtgctgttga gggcgttatc gagaatactc aggattcacc attaagtggt ttaactgaac
     1141 ttgcttttac tgatcgtcat ttagtttggt tagccaaaga ttggaatagt gaaaatgatt
     1201 tttatacctt aacaacaaat tatttacaca atggcaatca aaaagccatg attaacgcat
     1261 taaatgcttt ctttaatcca atgaaagctg acggaaggta ggaaaataaa atgaaaataa
     1321 aagaaatttt aaatcgttac tgggcaatca tttttgtagc aattccaatt attttgcaga
     1381 tgattttttt ctactttcca atggttcaag gtgcttttta cagtttaact aattggactg
     1441 gtctgactta taattttgat tttgtgggtt tgaacaatta taagattctt ttttctgatc
     1501 cgaagtttat caaatcgatt ggcttcactt taattgtgac gctttgcatg attgctggac
     1561 aaattggttt tggtattttg attgcgcggg cgttgaacgc aaatattaaa gggaaaacat
     1621 tctttagagc atggttcttt tttccagcgg tcttatcggg cgtggttgtt tctttagtat
     1681 ttaaacaaat ttttaactat ggtttaccaa aaattggtag tatgttaggc attgaatttt
     1741 tacaaaccag tcttttagga acaacatggg gagcgatttt tgcagtaatt tttgttttac
     1801 tttggcaagg aattgccatg ccagtcatca ttttcttagc gggttttcaa agtattccac
     1861 aagaaattat tgaagctgct agaatggatg gggcgaatga taagcaaatt ttttggaata
     1921 ttgaaatcac ctacttatta ccaagtattt caatggtatt tattatggca ttaaaaggtg
     1981 gcttaacagc atttgatcaa atttttgctt taactggtgg tggaccaagc gatgccacaa
     2041 catctttggg gttacttgtt tataattatg cttttaaaaa caatcagttt ggttatgcga
     2101 atgctattgc tttagtctta tttatcttaa ttgggattgt ttcactggca caattaaaat
     2161 tatcgaaaaa atttgaaatc taggaggttt cccaatgaaa acatcaaaag aaaaaaatat
     2221 ttctaaatac ttgcttttat tattaggggg cttcatgatt ttagttccct tagtttcaac
     2281 gctattcggt tcatttaaaa caacacccga tattatgaat aatttctttc gttttcctaa
     2341 tccagcgact ttggaaaatt tccgaagatt gttagcggat ggaattggtc attacttttg
     2401 gaattcggca atcatcacaa tcattgcagt agttgctgtg acagcaatta ttcctatggc
     2461 tgcttattca ctagcaagaa aaatgactcg tgggaaagca tacggattga tgtatttatt
     2521 tttaatttta ggaatttttg ttcctttcca agtaattatg attccgatta ccgttatgat
     2581 gagtaaaatt ggtttagcaa atatgtgggg attaatctta ctctatctca cttatgccgt
     2641 accacagaca ctatttttat atgttggttt tattaaacta aatgtgccta tcagtctgga
     2701 tgaggctgct gaaattgatg gggctagtca ctttacaact tatttcaaaa ttgttttccc
     2761 aatgttaaaa ccaatgcatg cgacgactat tattcttaat gctctatggt tttggaatga
     2821 tttcatgcta ccattattaa ttttaaatcg tgattctaat atgtggacac taccattgtt
     2881 tcaatataat tatacgggac aatattttaa cgactatggt ccaagttttg cttcctatac
     2941 agtaggaatt gtcacgatta cactagttta tcttgctttc caaagaagta ttatagctgg
     3001 aatgagcaat ggagcagtga aataaccaat aaattattct cattataaag gagttcataa
     3061 acttatgatt atatttgaca atcacacaca aacatttcat ttacaaaata aaaaaattag
     3121 ctatctatta ggaatcgaag aaaataatta tttaacgcat ctttattttg gaaaaaagat
     3181 tacgcactat tctggtgggt atcgctaccc ccgaacagat cgttccttct cacccaacaa
     3241 tgcaaatgcg caagatcgtt tattatcttt agacacatta ccattagaat ttccaggata
     3301 tggacatggt gattttagag aaccggcgca taatattaaa ttaggaaacg gtagtcgtgt
     3361 aaatgatttt cgttatgata gctacgaaat tcaaaaagga aagcccagct tagcgggatt
     3421 gccagcctta tatacggaag atgatgatga agctgaaacg ctgattattc aattgaaaga
     3481 cgcgattagt ggattgactt tacagttgtt ctacacaatt tatcaaaatc acagtgtaat
     3541 tattcgttct acgcaattag taaataatag tgatgaaaca gtggagatta accgcttagc
     3601 tagtcaatca attgattttc caaatcgtcc actcgagtta attcatttaa atggtacctg
     3661 ggcaagagaa cgtcaaatga ctagagaaaa aattcataca gggataaaag tattagatag
     3721 taaacgcgga tcaagcagcc atcaacaaaa tccgtttgtt gctttagtag atgccaaaac
     3781 agatgaattt caaggagagg catatggatt taatttagtt tatagcggta atcacgaaac
     3841 ggtgattcaa aaagatccat ttgaccaaac acgggtcatt atgggaatta attcatttaa
     3901 ttttggttgg caactaaaaa ctggcgactc atttcaaacg ccagaagtcg tgatggttta
     3961 tagtaatgaa ggtctgaata cgttatcaca gacgtatcat gaattgtata atcaccgttt
     4021 aattcgtgga aactatcgtt taaaagaacg tcccacgtta atcaacaact gggaagcaac
     4081 gtattttgat tttaacgaag aaaaaatcat gaatattgtc gatgatgcga agaacttggg
     4141 cattgaaatg tttgttttag atgatggttg gtttggcggt cgtgaaaatg acttcacttc
     4201 attaggtgat tgggttgaaa cagaaggcaa acttgcttgt ggtttggaaa atttagccaa
     4261 gcaagtgcat gataacggga tgaaatttgg gatttggttt gaaccggaaa tgatttctga
     4321 agatagtgaa ctatttcgag cacatccaga ttgggcattg ggtattccag gtcgtgaccg
     4381 ctcaattagt cgaaatcaat atgttcttga tttttcaaga caagatgttc gtgataatat
     4441 ctatcaacaa atgacggcga tttttgatcg tgtaccgatt gattatgtga agtgggatat
     4501 gaatcgaaat atgacagaag tctattctgt cttgttggac tcaacgatgc aaggtgaagt
     4561 ggctcatcgt tatatgttag ggttatatga atttttagaa aagttaacaa cgaattatcc
     4621 aactatttta tttgaaagtt gttcgggtgg aggcggtcgt tttgatgcag gtttcttgta
     4681 ttacatgcca caaacttgga cgagcgacaa taccgatgcc gtggctcgtc tgaaaattca
     4741 atatggaacc agcttagtgt atccaatttc aagtatgggt gcgcatgttt cagcagtccc
     4801 aaatcatcaa acaggaagag aaacgagttt aactattcgt ggcaatgctg cgatggctgg
     4861 tgtttttggt tatgagctag atctaacgca attaacttct gaagaaaaag cagaaattaa
     4921 agaacaagtt gccttttata aaaagcatcg ccaacttttg caatatggaa ctttttatcg
     4981 cttggaaagt ccgtttgaag gcaatgatac tgcatggatg tttgtctcaa aagaccaaac
     5041 agaagccatg gtcttttatt tccgagtatt agcagaagct gcgcatccat tagtcacatt
     5101 gaaattagca ggattagcag aagaagcaac ttatcaaatg gaagatgtaa catttgctgg
     5161 cgatgaattg atgaatctcg gtttttatat tgactcagaa ctacatggtg attatgcgac
     5221 acaacgcttt tatttgaaac agatatagta gcttgaattt acaatataag tgcaacacca
     5281 aaagagactc attccaaacg aagaaaatat gttttattta cacgcattta ctaaaaagca
     5341 gcctgatttg aattgggaaa atgaagaagt acgtgaagaa atctatgcaa tgattaacta
     5401 ttggttagat aaaggactag gcggatttag aatcgatgcg atattaaaca tcaaaaaaaa
     5461 actagagtat ggcatctttg aatctgatgg agaagatggg ttagccttca ttgggccctg
     5521 gattcttaat cagtcgggca ttgaagtgtg gttaaaagaa atgagagagc ggacatttaa
     5581 accacataat agtatgacgg tagcagaagc cgatgttcct aatgaacgtt tagatgaata
     5641 tattggtgaa gatggctatt attcgatggt ttttgatttt agttatacgg atattgatgt
     5701 acctgaaact ggtgaatggt ttaaagatag ccaatggaca tggacggata tgcgtaccaa
     5761 tatttttacg aatcaattag ttacgcaaga taaaggttgg ggcgccttat atttagaaaa
     5821 ccacgatcaa ccacgttcaa ttaataaata tattccagaa gaatggatca atgattatag
     5881 taaaaaaatg ttggcgacgc tttttatgtt actaagaggt acccctttta tttatcaagg
     5941 acaagaacta ggaatgacca acatagaaat ggcatcatta gaagattttg atgatgtagc
     6001 tacgcattct cagtataaac gggcattaga atatggattg gcaccagagc aagcattgaa
     6061 agctgtcaat aaacgaagtc gggataattc tcgcacacct atgcaatgga atacgcaaaa
     6121 aaatgccaga ttctcaactt ctgataatgt atggttaaaa gtaaatccta attatccaga
     6181 actcaatgca gaagctcagt taaccaatca acagtcggta ttcaattatt atcatcaatt
     6241 aattaaatta agaaaaaata gtgtatacaa agacgtgcta atctacggac aatttctacc
     6301 agtgaaagat aaaaatgaac atattttgct ttatgaacgc caattagaag ataaaatcgt
     6361 attagttatc ttaaacgtta cagcaacaga acaagaatat tatgtagatg aacagtacca
     6421 acaagtatta atcaataatt atgaagatgt tttattagat caaagtcgaa aattgcgttt
     6481 acgaccgttt gaaagtattg tattagctaa ttatggagga caattatgaa aattaaaaac
     6541 gaagcaatgc tcatcaccta tgcagatagt ttaggaaaaa acatgggcga attaaaacaa
     6601 gtcttagaca aacatttaca aggtgttatt ggtggtgtac atctgttgcc tttttttcca
     6661 tcgacaggtg atcgaggctt tgcgccgagt gattatacga cagttgatcc agctcttgga
     6721 acatgggaag atgtggaagc attaggtgaa gattattatt tgatgtttga ttttatgatt
     6781 aatcacattt ctcgtgaatc aaaatttttc caagatttca aaaaaaacca tgagcagtca
     6841 ccatacaaag aaatgtttat ccgtattcat gaatttttcc cagaaaatcg accaacacaa
     6901 gaagatattg atttaatcta caagcgtaaa gacaaagcgc cgtttcaaac ggtagaattt
     6961 gcggatggta cgacagaaga ggtttggaat acctttgggg aagaacaaat tgatttagat
     7021 gtgaccaaag aagttgtgaa agaatttatt cgagaaacca tcaaagatat ggcgaaccat
     7081 ggctgttctt taattcgatt agatgcgttt gcttatgcga ttaaaaaatt agataccaat
     7141 gatttcttcg tggaaccaga tatttgggaa cttttagacg aagtgcgtca agaagcagcg
     7201 aaatatgacg tcgaattatt accagaaatc catgagcatt attccattca aatgaaaatt
     7261 gccaatcatg attattatat ctatgacttc gccttgccaa tggtcactct gtattcactt
     7321 tatagtggaa aatcagaacg tttagccaat tggttgaaaa tgagtccgat gaagcagttt
     7381 acaacattgg atacgcatga tggaattggt gttgtggacg ctcgtgattt attaacagat
     7441 gaagaattgg attacacctc tgaggaattg tataaagtcg gggcgaatgt gaagaaggtc
     7501 tattcaagtg ccaattacaa taatttagat atttatcaaa ttaacagcac ctattatagt
     7561 gcattaggcg ataatgaccg tagctatctg ttagcacgtg cgattcaagt atttgcgcca
     7621 gggattccac aaatttatta cgttgggtta ttagcaggaa aaaatgatat tgaactgttg
     7681 gaagcaacga aagaagggcg taatattaat cgtcattact atgatttagc cgaaattgaa
     7741 gaagaagtgc aacggccagt gattcaacaa ttatttgcac ttttaaaatt tagaaatgaa
     7801 tctgcggcgt ttgatttgga tggaacgatt gaagtagcaa cgccatcaga atcagaaata
     7861 gtgattacac gtaaaaataa aaacggtagt gatagtgcaa cattaacggc taacttggat
     7921 acgaaagaat ttatcattga gtctaaagat ataaaaattt tcactagtgt tgcattaaaa
     7981 ttttaaccta ttccatagaa caaaaaatcc tttatactag cacttggatg acttatccaa
     8041 gaccaataaa aaggactcat acatggataa gtataaaaca aatacagcat ttaataaatg
     8101 gttttctgcc ataaaattag gaaaactttc tgtcaactcc caaaaatgta tttttgatta
     8161 caattcgtat cgtaaaaaac tgtcttttga agctgtgctc aagctctttc tctacgcagt
     8221 taatgaagaa aaagaaagtt tgcgtgattt gagtacagct cttatcgacc gatcgctcca
     8281 actagaaacc gatgtgtcat cgattagtca tacccaattg tctcgagcat ttaatgcact
     8341 tgaacctaaa gtgttagaag aaatttttca acaactgtta gaaaaagttc aacatcaaat
     8401 caaaccaacc aagcgaaatc atctgtatct gattgattcc agtacgtttt cgcttagttt
     8461 aaaacgtcat aagtgggcga ctttccgtca aacaaaatca ggtgtcaagt tacacttaaa
     8521 ctattgttat atggacgata ctacgatgta tccaactgat tttaccttaa caaatgccca
     8581 tgaacatgat ctcaatcaac taccagtatt ggtgaatcaa ccagaggcaa cttacgtgtt
     8641 tgatcgtggg tattaggact ttgaaaagat ggatcagatg cattgggatg gctatttctt
     8701 cgtcactcgg attaaaaaaa acaccaaagt tcacgtgata gatactctgg aaacatcgcc
     8761 agaaacagaa attctacgag acgagctggt tcgtttagga tctaaaacct atctaaccgc
     8821 caatttccga ctagtcactg ttcaagataa gaacggacgt gtgtttcaat ttatcaccaa
     8881 tcgaatggac gtctcttcaa aagaaatctc tgatatgtat cacgctcgtt ggcaaatcga
     8941 attgtttttc aagcacatca aacaacacat gaccattaaa accttctttt ctcaaagtga
     9001 aaaaggcgtg cagaatcaat tgattttaac gatgatttcc gctctcctca cgtttctcat
     9061 caaattggag acgaaaacgg aaaaatcagt gttccaaatc aaacgattct ttcgttattt
     9121 actgtttcag ccctttgaat gttgtctaga aaaattaatt cccacatgag aaaaaagaaa
     9181 tttggctcgt tacttttggt tttcagttga ataacagata aaaaattttc tggaaaaagt
     9241 catcctctgc gtcagcacag ttttttttga cttattttca gaggaaatag ttttcagaat
     9301 attctaaaat gttttatgca acactagtga tattttttag aattagattt ctaatgtgca
     9361 ttttttgagt attaaggagc aagtatacaa agatttataa agctgattaa atttgaataa
     9421 aaatatgcag gaacgctact atttttagta gtgttctttt ttatatttga ataaaaaatg
     9481 tggtggttaa atggtggtta taacaaaaaa tgaattaatg aaattaggtt atgggaagta
     9541 tcagtctgag agaatcatac gacaggcaaa agcaattatg gtttcaaaaa gctataccta
     9601 ttacgaaaat agaagattag gactagtacc tgttgaatct gtagaagaag ttttaggtat
     9661 gaagatagag gtagaggagg attttcagaa tcatggttaa ttttaaaaaa gataaaaatg
     9721 ggaaatatca ttttactgct agtttgggat tcgacaaacg aacagggaaa agagtacaaa
     9781 aaaacgtaga ggggttaaaa cagttaaaga agaaaaaaag cgtataccga acttgtttct
     9841 aattatggaa aagaagcag
//
LOCUS       DACDUU010000091         9830 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.091, whole genome shotgun sequence.
ACCESSION   DACDUU010000091 DACDUU010000000
VERSION     DACDUU010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.091"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            508..1071
                     /locus_tag="IWL30_002500"
     CDS             508..1071
                     /locus_tag="IWL30_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707219.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(1132..1497)
                     /locus_tag="IWL30_002501"
     CDS             complement(1132..1497)
                     /locus_tag="IWL30_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707220.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1573..1818)
                     /locus_tag="IWL30_002502"
     CDS             complement(1573..1818)
                     /locus_tag="IWL30_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9707221.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1888..2142)
                     /locus_tag="IWL30_002503"
     CDS             complement(1888..2142)
                     /locus_tag="IWL30_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707222.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(2306..2629)
                     /locus_tag="IWL30_002504"
     CDS             complement(2306..2629)
                     /locus_tag="IWL30_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707223.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            2816..2889
                     /locus_tag="IWL30_002505"
     tRNA            2816..2889
                     /locus_tag="IWL30_002505"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2850..2852,aa:Ile,seq:cat)
     gene            complement(3274..3771)
                     /locus_tag="IWL30_002506"
     CDS             complement(3274..3771)
                     /locus_tag="IWL30_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9707224.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(3811..4620)
                     /locus_tag="IWL30_002507"
     CDS             complement(3811..4620)
                     /locus_tag="IWL30_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9707225.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            4954..5811
                     /locus_tag="IWL30_002508"
     CDS             4954..5811
                     /locus_tag="IWL30_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP9707226.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            5804..6706
                     /locus_tag="IWL30_002509"
     CDS             5804..6706
                     /locus_tag="IWL30_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP9707227.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            6739..7089
                     /locus_tag="IWL30_002510"
     CDS             6739..7089
                     /locus_tag="IWL30_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9707228.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            7123..8061
                     /locus_tag="IWL30_002511"
     CDS             7123..8061
                     /locus_tag="IWL30_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP9707229.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            8139..9032
                     /locus_tag="IWL30_002512"
     CDS             8139..9032
                     /locus_tag="IWL30_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9707230.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            9070..9633
                     /gene="amaP"
                     /locus_tag="IWL30_002513"
     CDS             9070..9633
                     /gene="amaP"
                     /locus_tag="IWL30_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP9707231.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            9647..>9830
                     /locus_tag="IWL30_002514"
     CDS             9647..>9830
                     /locus_tag="IWL30_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP9707232.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFK"
BASE COUNT         3419 a         1663 c         1849 g         2899 t
ORIGIN      
        1 tcattggtcg ttgaatgggc tgaatagtag tgtataaaac aattttgtgg agcgtgaagc
       61 ttttatggta gaggaaaata cgaataaagt gatgttgata aaaagattcc tatcttgaat
      121 atgaatcgag atgtcttgaa aatgaatgag tttgaccaat cattatccta tcgaaattta
      181 cagtttcgtt cgattggtct gtcaattaat gaaaaagtaa aaaagaatat gttctaggaa
      241 gagggcggca catctaaatt tttaagtaac ggacagttca aatatcaagc attgaaagag
      301 gctatcctac tgtttgaagg aatacgaata tataaaaacg acaaagagga aaaagtagtt
      361 cattaaatcc cttcaagtga tgaaagaatg agatctaata aaacctgatc tttcatctgt
      421 ttttttaata acgtaataat ttttttattt tttgtgcttt cgccgttatt gtagagtgat
      481 ttagcattct tatttttaaa aataactgtg ttatatatta aaaaaggcat aatagttata
      541 agctttttta aagaatattt agaaattatt aattttcgtc tgaaaacaaa aaaagaaagc
      601 gttattattt tagtctattt tcaggggggg aaccttatga taaaaaaact atttttacta
      661 tcaatattaa cgctagcaat ggcaggttgt tctgactctg gcttagcaac tgaactgatc
      721 acaaaatcaa ctgaagccac gacagaggag ttaaaatgtg gaaacgatca cagtgactac
      781 agaggcttaa atcgacgatg catgggaata ttaggcagaa gcaattgtaa gcatcgatgt
      841 agtaatcgaa gaaggtgtag aagaagcaac agaaaaatca aaagaaagat caaaagaagc
      901 aatctgtgct gtgcatcgta tacagacgtc gaagatgcgg tacttttaaa ccacgtcagg
      961 agatgttcat ttatgtctag ggaagttggc gatgaaccaa aactagagta cgcttttagt
     1021 tttgcacgtg aggatgaaga taaccctaaa acttacgaca ttttagcttg atctaagatg
     1081 gttaaagctt tttaatgaag taagaggaga tagaaggctt ttctagttat attaggtaag
     1141 ataaattcct ttttgctcca aatttgctaa acattctgct agataaactt catcgtgttc
     1201 cggataaatc ggcacgttgc gctctacagt aggtaaatca aaaaatgctt cgcatgattt
     1261 gccacgatac ataggatttt tccactctgg tgctgggctg taaccacgtt tttccatttc
     1321 atccatgaca agctgatgat attggaataa tctataagga gaatagtgaa aaacataatc
     1381 cacagtagca tgtttctttc cccagccatt tcctcttaat gcacagcatt cccgatgttg
     1441 tcctaaaagc tgttgacgag gaagctttga aatcaatttt tcatgccata atcgcataga
     1501 aatgtctcct ttatatatag attgttaacc ttagacgtta aatgaaaaaa ccagcaccaa
     1561 ggctggaaaa aatcacttca gtttgcttgc tgtttctgag cgaccaatta ggtaatcaac
     1621 ggaaatatcg taaaagtcag cgatatagat taattcaatg atgcttggaa tttcttctcc
     1681 ttcttcgtac ttacgatatt tgaataaagg aatacttaac gcgctagaaa cagctacttg
     1741 agataaagat cggccagttc ttaagaaatt cagacgacaa cgtaaagtat ccagtaaaat
     1801 agcttcttcg atactcattt aatcaccttt cattatagta gaatagttat aatgactata
     1861 gaaagctaca catgcaatca atagtactca atgtttttta tttaaacgta tcaaataaag
     1921 aatgacgatc aataaagaca aaaccagtcc tgataattct tctataaaaa cactatcggg
     1981 ttcaaagtag ttgtaattaa aaactttcca cataaagtag atagccggaa gagtcaaaat
     2041 agctaaataa aaccgttctt tgttccaatt cataagataa acaatacttg cgaccagtgc
     2101 gaccgcaggc acccaaaaca aaactctcac caacgtaatc atgtaacgga ctcctcaacg
     2161 attcatataa taaaagataa catgttattg catacatcac aaatgaaatg ctaggaaaat
     2221 aaaagaagaa tagatcaatg aaaaatgaaa aagcagaaca gttttctgct gataagcagg
     2281 aactgttccg ctgaatgtgt cttatctatc gagaaaaaat caatggattg atatctaagt
     2341 aattcaatac atataaaaag aggacgatcg cattcactaa aaaggaaaga atgatgatcc
     2401 catagcgaat ggaaacatat ttaacaaata cttttgtttg aatacgggca tatctttttc
     2461 ctttatagct gagcatcatg acaagaagca aaagggtaat cacaccttga aaaatcattc
     2521 caataaacag aattcctgag gtttgataat ttaattggag aaaataagct gctgccgcaa
     2581 ctaaatcgaa taaaatcgca ataaatggca aaataatcac tcctaacatt gagaataaat
     2641 aaaagaataa cattttttct aaaataagca aataaaaatt aaagtaacaa aatagttatg
     2701 acacaagtaa aagaaataag aataacaata ttattttact tatattttga aacttgtgag
     2761 agtttgtcat tcgtgctata gttgaaaagt aacaaaaata aattttattt atgatgtacc
     2821 tttagctcag ttggttagag cagacggctc ataaccgtcc ggtcgtaggt tcgagtccta
     2881 caaggtacat aagaggttgt gaaaaagctg ccctgcatca agtaataaga acagccaagt
     2941 aaaaacagct tcttatgttt ttacttggct gttcttatta ctgcagatgc aagcttttga
     3001 ataccgttta aggaagaggt tgtgaaatca gcgttttgac ctgagtatta gcttaacaaa
     3061 cggaaaaata gttcctcata ttttgccgtt tgttaagcta ataccgaggg tcagctgatt
     3121 gaacaccatt taagaaagag gtcgtgagcc tgccgtttca ctctgtgtca taaaaaagcc
     3181 aatctacttt gatagaaaaa gcacgtagga ctaaaacagt tctgcgtgct ttttttattg
     3241 tgtgttaaat atcgtaaatg aagatagata tttttatgat tcgacgatag agacgttgaa
     3301 atctttcaac agctctaaag cggattctcc gtgttttcta ctggagacta acattggatc
     3361 aacaaaaaag tctgcttcag gaaaagcact ctgcaggcct atcagattag agagtacaca
     3421 caagtttgtt tcaatcccga ctacttcgat ctctttttgt tcttctttgc tttcaaataa
     3481 agtttcctgg atttcagcta aagtttcagg tggaatcgta aagtaatact ttttaatcac
     3541 tcgttcattt tcttgtggag aaagaagagg gatcaattgc aaatcttctc gttcttcttc
     3601 tttgtccttc agttcaactg gtatatcccg tgtgaagaga atatattcat tattttggcg
     3661 tgctttagct aatctttgag caatacgttc tgccaattga tctgactctg gaatataaaa
     3721 ttcgctgttt ggatcaagaa tatgattttg catatcaata ataactaaca ttttctttcc
     3781 tcctacattc atatcgtcat tttattcttt ttaatagaag ttttttaaaa tataagcaac
     3841 accagcttct tcattcgtca gtgttacttg gtttgcaatt tcttttactt gtcgctgtgc
     3901 attccccatc gcaatactca acccgctgta attcaacata gcaatgtcgt tttcttgatc
     3961 gccaaaagcc atgatgtcac tcgattcgat tccaaggtaa tccgccagaa aaacaagtga
     4021 atgagcttta gtgatacctg taggcgtgat ttctaagaaa tgcggatcag agaatactga
     4081 ttggatacca tctgcttgca gatctctttg tagcttctgt agtttttctt cactcttatc
     4141 tgttaatgct aatttaagat agctaagaga aggatcttcc aacatattta aaatttgatc
     4201 aaaagaatgg ttctgtaaat tgaaatgttc ataataaggg tcatctttga ctagatttga
     4261 ttcatcgaat gtcagctgat catcttgggt ttctcggcta gcaatgatgg atattttcgc
     4321 ttcttgagca tagaagaagg cttttctcag tgtatcgcta tctatttttg aacgtctgag
     4381 aactttcgcc atttttccat ccttcatttc agcaatcagt gaaccattgg aagaaatcaa
     4441 atagccaccg attcctaatt cggaaaaaat atctttagca gaaatgctgt tccttccggt
     4501 agcgacggtt acgattccgc cattttcatc aaatttccga agtgcttgga tattttcttc
     4561 tggtatcttt ccatttcgat caagcaaggt accgtctaaa tcaatcgctg ctagtttcat
     4621 tttatcttcc tactttcttt ttacatatct attttcctgt taccttaaga ttaaatgaga
     4681 atttaaaaaa atgaaactaa cttgctttgt cattatgaat tttttcaaaa aacattctta
     4741 agaaaaaggt ggctatatat agtagttttt ggtcaatacc tttgaataaa aagccatgaa
     4801 aaacgaatag ttagcgctta ccgatgcgtt ttgtgataaa aaaattttaa aatattcaat
     4861 gaaaacaaag catatatttt gctttaaaac caaatgagga atattctaga tgtagaacag
     4921 gttagaaaaa gattagaaaa aggagtgatt ttgatggaga agagatacgg cgtagttcct
     4981 gtggtaactg cttcagacga aaattatgct ccttacttga gcgtgatgat cgcaactgca
     5041 ttagaaaatt gcaataaaac aagacgcata aaattttatg tgattgacga tggtctatca
     5101 gaatatagta aagaaggatt ggaagaaacg gtaaataaat attcaagtaa tgcaagtatc
     5161 caattcctga cagtggaaaa agatatctac gaagacttcc tagtcagtga ccatatcaca
     5221 actaccgctt acttaagaat ttctttacca aacttattag ctaaagagga ttataagaaa
     5281 gtcctttact tagattcaga tgtgttagta ctagacgata tcgtaaaact atatgatgag
     5341 ccgttaaatg gaaaaacaat cggcgctatt atcgatccag gccaagtgaa agctttggaa
     5401 agattaggaa tcgattccga tgacttgtac ttcaattcag gagtgatggt gattgacatt
     5461 gatcaatgga ataaaaaaga aattacagaa aaaactatcc attacctcag tgaaaatggc
     5521 gatcgaatca tctatcacga ccaagatgct ttgaatgctg tactttatga agattgggaa
     5581 caacttcatc caaaatggaa catgcagact tcgctgattt ttgaacgtca tccagcacca
     5641 aatgaaaaat atgaacgtca gtataaagaa ggaaatgaaa aaccttcgat cgttcacttt
     5701 acaggacatg ataaaccttg gaatacgttg aaagatcatc cgtataccaa tttatactta
     5761 aaaaaattag cacacagtac tctaacgaaa gtaggcgaga tcaatgaata aaaaagaaat
     5821 agcagtagtc gcaagttgta acacaaaatt cgtaccacat ttagctgcat tatttgtctc
     5881 agtattagat aattgcaacc cgtctaaatt tgttcggttc tatgtgattg acgatgatat
     5941 cgattttgaa agtaaacagc ttttacgctt ctcagtgaaa aacgcacgaa tgaactcaga
     6001 tgtggaattc ttgaaaatca ataaagaatt ctttacaaat gtggtaatca gcgatcgtat
     6061 tccagaaaca gcttattatc gtattgcgat tccggaattg ttccgcggca cagaagttga
     6121 acgtatctta tatatggatt gcgacatgat cgctttacaa gatatctcta aattatggcg
     6181 tcttgacttc ggtgattcaa tcgtagcagc tgttgaagac gctgggttcc atcaacgact
     6241 ggaaaaaatg aaaatttcag ctaaatcaat gcgttatttc aactcaggtt tgatgctgat
     6301 caatgtgaaa aaatggttag atgaaaatat tacgcaaaaa gtattagatt tcatcgaaca
     6361 taatccagaa aaattacgat tccatgacca agatgcatta aatgctatct tacatgaccg
     6421 ttggttacca cttcatccgc gttggaatgc acaaggctat atcatggcaa aagcaaaaaa
     6481 acatccaaca gcagcaggtg aacgtgaata cgaagaaact cgcaacaatc cttacattat
     6541 ccatttttca ggacatgtga aaccgtggag caaggatttt gaagggccaa cgaagaaata
     6601 ttacgaaaaa tatgcaggga tgacggcttt ccgttgtgta gctaaattcc ctaaatatcc
     6661 aaaatatgca aaagttcaac caaaaacaga tgtagttgtt aaataataga agacaacaac
     6721 tgaagagggg gagtaggaat ggaaattaca atgtctaaaa atgcagtaga gacgttaatt
     6781 gaaaaggttg gggaaaatac aaaaatcgca ctcgcgctta tcaatgattc cgatccattt
     6841 ctaagggaca aaggtgcttt tgcaaaaggc agcttttttc aaatcattcc ttttgtttcg
     6901 gaatttggag aatatgcgac taaaatcgag catccattat tggacatcta tacatctaaa
     6961 ttagagcaaa attatttcgg taaacgattg aacatggact tcaacaaaca gcttgacagc
     7021 ttttcattag aaaatgaaat cgcagtactc gattataata tcaaattaaa gaactgcttc
     7081 ttcagctaat agtttcgaga aagtccaaaa aggaggtata cattgagata ctactttgtg
     7141 gatgaagaaa agaatgtaat agaaacagat gaaaaaaaat ataattggct cgtacttgat
     7201 agtgcaaagc ccgaagagat tgagcaagca atcgaagaat tcgagcttcc tgatgatatt
     7261 ttcgtaggaa ccgaatatcc ggaagaagtt tctcggatgg agcgccttta tgagacgaaa
     7321 ctagagcatc cattttcttt ggtagtgatt aatctagatc agtcagataa tcgtatcgaa
     7381 aaaaaattaa cgccaatatc ttttgtactt tccgataatc tcttgattac ttgtatcgat
     7441 agaaaaaccg aatttgtgga tcggctgctg acaaagcatg gaaatcattt aaactcgttt
     7501 gaaaagatca tcacgtatac attattagat atttacaccc attacgtgaa agaactaaga
     7561 gagatgaaaa agcgaattga cgctttggat aaagcagctc gtaaaacaac ggaaaatgaa
     7621 gaactgtata aacaggcaga tcttgaaaga gacatcgtat atatcgatca taccttaaga
     7681 gatcaacggg aaacaatgga tcaattatgg aattctgaaa ctttcatcaa acgtttagac
     7741 gatgaacagc tactctatga tgtccatttg agacagcgac aaacagaaaa aatgatcgag
     7801 atctatcgag atttattaga gagtattggt gatctattca acgggatgat ggacaataat
     7861 ctaaatcata tcatgaaata tctagattca gctgcattaa tcatttccgt tccggcgttg
     7921 ttcgcaggga tttggggaat gaatacagga ggattaccgg gtaaaagttc tgctctaggc
     7981 tttatactcg tagtctttgg ctctttgata gcagcagtag ttttaggtta ccatttatac
     8041 cggaaagatt atacaaatta aagaatctat ttaaaacagg tatcaaacaa agaaatttgt
     8101 tttggcaata caatttaaaa actaggagga atacagcaat gacagaacca aaattaatcg
     8161 acccgcgaaa cttgtaccat acggagaaat ttcctaaaca agatcaggat acaccagctt
     8221 tacagcagaa tatgattcct gttcctgatt gtggagaaga atcctatgaa ggcaataatc
     8281 aactagaaaa ccgccgtgta ctaatcactg gtggagattc agggatcggt cgtgcagcag
     8341 cgattgcatt tgctagagaa ggggcagata tcgctcttca attcttccca ggagaagaaa
     8401 aagatgcaga ggaagtagca aaatacgtga aagttgcagg acgtaagatt gtcttactac
     8461 cttttgatct tcgtgacgaa caagcaccta aagaaatcat agataaagcc gtgagtgaac
     8521 ttggcgggct agatacgatg gtactaaacg caggacagca aatttcttgt cagtctattg
     8581 ctgagttacc acttgagcaa gtcaaagaca catttatgat aaacatcatc gctatgtttg
     8641 ctttagtgaa agaagctgtt ccgcatatcc cagcaggagg agcaatcgta acaacaactt
     8701 ctgttcaagc attcaatcca agcgaacatt tattagatta cgcagcaaca aaagcttcaa
     8761 tcgctaactt tacgatcggt ttagcaaaac agcttgcacc taaaggaatc cgtgtaaatg
     8821 gtgtagcacc tggtccaatc tggacaccat tacaactaga cgaaggacaa ccttctgatt
     8881 cgatccctga atttggtcag caaagtttac tagaacgtgc aggtcagcca ggagaattag
     8941 caccagttta tgttttctta gcatcgaata aagcaagtta tgttacagcc caaatttacg
     9001 gagtaactgg tggagaagcg atcaacttat aagaagttga tcgtttcacc cctatgagga
     9061 ggtgcgaaaa tgaataaagg tgtaaaaacg atcggagtga tcggaacatt acttttgcta
     9121 tctgtgcttt tgtttgcgat actattaagt gcaacctatc cattgccttt tcagctagag
     9181 aagtttcgat tcttttccat cactaattca tacgtgcaac aatatgtatt ttggcttgcc
     9241 gttgcatttg ctattttgac aattatcacg ttacttgttt tcctgtttta tccaaaaaca
     9301 atcggaaact ttctactgaa acatggtgat ggggagttga cgttggataa aaaagcaatt
     9361 gaaggaatgg ttcgttctca cttgcatgaa gatgaattca tccattcacc aaaagtaaat
     9421 gtcaaagcaa cgaaaaacaa gatcgccgtc aatgtaaaag gggagttgaa acgaacttca
     9481 gcactcattg gtaaaacagg aacattgatg aaagaaatcg aaaatgaagt acaacacata
     9541 ttgggaacaa aagagccagt gaaagtatcg gtgaaataca caggctttga agaaccagct
     9601 aaacgaagca atgaccgcaa acgagtagaa taggggggga ccaaaaatga aagaactatt
     9661 ttcgacatac aagctcccta tcatctttgg ggtacttgga ttgatattag ccgttttatg
     9721 gatcaccgtg ggctttttca agaccttatt gatcttgatc atggcagcga tcggcgtcgc
     9781 tatcggtttt tatctgaaac aaacgaaaat cttagatcac tattttaaat
//
LOCUS       DACDUU010000092         9626 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.092, whole genome shotgun sequence.
ACCESSION   DACDUU010000092 DACDUU010000000
VERSION     DACDUU010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.092"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(55..1365)
                     /locus_tag="IWL30_002515"
     CDS             complement(55..1365)
                     /locus_tag="IWL30_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HAP9707233.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            complement(1397..2221)
                     /locus_tag="IWL30_002516"
     CDS             complement(1397..2221)
                     /locus_tag="IWL30_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9707234.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            complement(2237..3133)
                     /locus_tag="IWL30_002517"
                     /pseudo
     CDS             complement(2237..3133)
                     /locus_tag="IWL30_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378728.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            complement(3216..4325)
                     /locus_tag="IWL30_002518"
     CDS             complement(3216..4325)
                     /locus_tag="IWL30_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP9707235.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            complement(4619..5644)
                     /locus_tag="IWL30_002519"
     CDS             complement(4619..5644)
                     /locus_tag="IWL30_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9707236.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            complement(5889..7160)
                     /locus_tag="IWL30_002520"
     CDS             complement(5889..7160)
                     /locus_tag="IWL30_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAP9707237.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            complement(7444..8391)
                     /locus_tag="IWL30_002521"
                     /pseudo
     CDS             complement(7444..8391)
                     /locus_tag="IWL30_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            8403..8876
                     /locus_tag="IWL30_002522"
     CDS             8403..8876
                     /locus_tag="IWL30_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9707238.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            complement(9049..9603)
                     /locus_tag="IWL30_002523"
     CDS             complement(9049..9603)
                     /locus_tag="IWL30_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9707239.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
BASE COUNT         3041 a         1870 c         1437 g         3278 t
ORIGIN      
        1 tttaactaag aaaagagtaa ggaattgaaa attccttact ctttcagctt ctttttaatt
       61 atattcaggt aaatagtccc catgtgcttc gattaaatca tccactaaat ttttgatatc
      121 tgttaaagac aattcagatg atagatgtgg atctaaatac gctgcttgat aaatttttga
      181 tttatctagt gttaaagcgg cttctactgt cagttcttga acattgatat tcgtccgatt
      241 taaagcagct aattgtgttg gcaattcacc gacataggtt ggttgcacgc catttttatc
      301 gactaaacaa ggtacttcca cacaaacatc tgtcggtaag tttgtgatta agcctttatt
      361 taagacattt cctgcaatca ctgacggagt tcctgtcgta atagaatcca tgatatatga
      421 agcatattca cggcttctcg tatgtgttaa ctccccatta tttacaatgt cctctcgcat
      481 gctttcccaa tcattaattt gttgttcaca acgacgtaag tattcatcca acggaatccc
      541 taattcgtca atcaattctg ggtattttga tttaataaag tatggatggt attctgcatt
      601 gtgttcactt gattctgtaa tatagtaacc aaaacgtttc aacaattcaa aacgtacaga
      661 atctggatgt ggatttggtt tttctgcagc taactgacga attgttggat agaaatcttc
      721 cccatttttt gtaatttcca acaagaatgc catatgatta atgcctgcaa ttttccattg
      781 aaagtcattt aagtcatatt gatcggctat tcctaaatgc tcaaacaatt ctggtacgca
      841 tacttgaacg ctatgacata aaccaacagt cttaatactc gtcgctttta aaacacccat
      901 ggttaacatc gccatcggat ttgtatagtt caataaccaa gcatttggac aaacttcttc
      961 catttcttta gcaaagtcta acattactgg tagtgtccgt agggttctaa aaatcccacc
     1021 aatccccatg gtgtcaccaa tagtttgtcg caacccatat tttttaggaa tatcaaaatc
     1081 aatcactgtt gaaggtttat aaccacccac ttgaatagca ttgataacaa aatcagcctc
     1141 tcttaacgct tctttgcgat cataataaga aacaatatca gcacgatttt cattcgaatt
     1201 tttattaata tttttaagca ttaaatttga ttcttttagt cgtttttcat caatatcgta
     1261 caatgcaata tgtgcatctt gtagacttgg agttaacatc gcatcgccta atacgttttt
     1321 tgcaaaaata gtacttcctg caccaataaa tgtaattttt gtcatgtact tactctccaa
     1381 tcactaatta atagttttat aatttactac ctgttgtagc aattccttca atgaattttc
     1441 tttggaaaac gatgaatatc acaactattg gccaaattgc taataacgaa cctgccatta
     1501 attgtgggaa gttcgtggca tattgtcctt gtaagtttgc taatcccgga gctaaaggca
     1561 tcttatcaat tgaagtattg acaatcaatg gccacattaa atctttaaat gcaaacaacg
     1621 ctgtgaatat tccaagtgca accaatcctg atttagcaag cggcaacata attttgaaaa
     1681 agatcgtcca aatattggca ccttctaaat aggccgcttc ctccaattca tcaggtaaac
     1741 ctaagaaaaa ttgtcttaat aaaaacgtac caaacgcact aactacccct ggtaatatta
     1801 atgctggaat tgtatttaaa atacccaatt tttgtgctaa taaatactgt ggagtaataa
     1861 aaatggaacc gggaatcatc atttgaataa gtactaagct gaaaaggata tttttccctg
     1921 gaaaacttag acgcgcacaa gcatatgcag ccattgctga aaataaaacc gagccaatca
     1981 ctcgtccaac aaccattaaa aatgtattga tataaaaagc catgaatggt aataaatcaa
     2041 gtacttgttg atagttactt agctgaaatt tctcagggaa aattgtaggt ggaatttggg
     2101 tagattcaac caacgttttc cctgaagtta aaatcatcca aataaaaggt aatgccattg
     2161 caataccacc taaaataagc atgatatgga ttggcgcatt tgaaaaaaat ttatttttca
     2221 taatttgata gcctccttaa tagtttaccc actttttctg caacttcatt tgaattgcag
     2281 taataattaa tataatcact aaaaggaaca taacgattgc cgaaccatat cccttgtcat
     2341 ttaaaataaa tgaatgttta tagaataaat aggctaatga ttgtgattta aacatggcgg
     2401 tactattagg agtaatcatc ataaaaataa catcaaatac ttggaaagca ttgataactg
     2461 ttgttacaac tacaaaaaat aatgttggtg taactaatgg taacgtaatg ttgaaaaact
     2521 gctggattgg actagcacca tcaatttccg ctgcttcata ataatcttta ggaatttctt
     2581 ataagccggc tagtaataac accatgttat aaccaatagc actccaaact cctacaatga
     2641 taatagaata aatcgcaatc gatggatccg ttaaccatgc tggtccatcg agacctattt
     2701 tagccaacat gttattaatc agtccataat cattattgaa catccagcgc caaatcattg
     2761 cgactgcgga tggtgcagca acgactggta gaaaataaat tgtccgataa atcgacaatc
     2821 ctttaacttt tctattcatt aaaactgcca ccaacaagga taatacaaca atactaggta
     2881 tcgaaaccac tgcataaatt aatgtgttta ataaagactg aaaaacttca ccgtctgcaa
     2941 acattttttg gtaatttctt aatccgaccc attcagtatc accgaaccct accgtttttt
     3001 gaaaacttaa aattgctgtt tgaattgctg gccatatatt taataaaatt aatcctaatg
     3061 ccgttggaaa aataaaaata tagccccata tcacttgatc tttacgatat tttccaaaaa
     3121 ttcgattttt cataatatcc ccctttttgg tattcatcca ttaatataca gacgaaatag
     3181 aatcctttcg cctgtatatt gtataattaa ctaatttaat ttccgctcaa tatcgcattg
     3241 acttcttttt caatttgagc ggttccatct tcaacagtta cttcaccaga cagaatacgg
     3301 ttaataattt gaacttctgc ttgttcagct cgtaatccat ctttattgta tggacgaggg
     3361 actccatact ccaacatttt tccaaaagca ctcaaatcaa ttgtatctga agaagcagcc
     3421 cactcgtctt caaagccttt tagtgctggg attgatgccc ccgtttccgc ttggatttta
     3481 ttggcgtcct cactagctaa aaatgccatt agttttttca cttcttctgg gtattttgta
     3541 ctcgcactgg cagcccaacc aagtccattt gtaattgttg cattaacttt tcccttaggt
     3601 aacacggcag aagcaacatt ttcaactaga tagtcatttt tcaaaaatcc cggtgcaaac
     3661 cagtttcctt caatcatcat tgctaatcta cctgattgaa aacgacttaa cttatcagga
     3721 tcttgtaatt ctgtaattgt tggtgaagca tgatcaacaa gtgcaatatc tcgataaaaa
     3781 gctaaagctt cttgtgcttc tggggtattc aaacctgatt ttgtattgtc atcggcaaat
     3841 acttctcccc cattttgata aataaatggc cagtagccag tctctgttcc aatatgtgat
     3901 gcaaaaccat agatgccttt tgattcatct gttaaatcaa ttgcagcttg tctgagtgtt
     3961 tcccatgtcc atgtttcatc tggataatcg acacctgcat tatcaaataa ttctttgttg
     4021 taccatagcc ccactacaga agcatcttta gggattgcgt atagttcgtc ttcaagcgta
     4081 tagaaatcag caattccttc agtaaagttt cccatgtcaa tatcattttc tttgataaca
     4141 tcatcaagtc ccatcaaagc attctgtgac atatatttat aggattcgct agagtgcatg
     4201 gttactacat ctggcatatt acctcccgtt gttgcagctt ctaatttaac ccaatattga
     4261 tcccaaggcg ttgtctccat ttctactttg atatcaggat tttgtttttc aaattcatct
     4321 aacatttttc ttagtcctgg agcttggtca gtatctcaac tcgcatagct taaagttact
     4381 tgcccgtctt tagaattact atctccccca ccacatgctg cgagtaatgt cccagttaaa
     4441 acagctatcc ctgttaaagc tatttttttg aaatgtttca tcttaattcc tcctgttttg
     4501 aatgcgtttt ctttttgtta acaacaccat aactgtttac ttaatgtttt gtcgagattt
     4561 tttactaaaa atattagtaa acaaaaaagt aaacaccctt ttttggatgt ttactttttt
     4621 aatcgatatt tttttcactt tctctaacaa taatattatt agaaacaatc actttgatcg
     4681 aaacatttct aacttctttt attcgttcat taaccatgcg aactgctagt tttccaattt
     4741 cttctgtatt cacattcacc gtatttaatg caggtgttaa aaattcagca acttcaatat
     4801 tatcaaaact cacaatcgaa atatcttctg gaatatgtag accatttttt tgaagtgaac
     4861 gataaacccc cacagcaata ggatcatttc ctaccatgat agcagtcggc aacttatctt
     4921 tagcatgttt tttcaaaagt aaatctgctg cctgcattcc gtcaagtgtg gtccagcctt
     4981 ctaaataaat ttgactatat ttttctaatc ctttttgttt catccacgtc tcataggcta
     5041 tttttcgata atcatcatct gacacgtgtt tatttccttg gctatctaaa cgaacacgtc
     5101 ctcctccgat aaacgcaatg tttcggtgtc cttttttata caatcgatct aaatgctgat
     5161 tcgttgcttt tgttaaatca gtataaactg catctacttc agcttcaata ctagggtcat
     5221 caatcactac gacattcgaa ttaattgctg atacttcttc aatcacagaa gatagcacct
     5281 gcccaataat caacacacct tgacatttta gcaaatcgtc ttgctccaac ggtgtttctg
     5341 acaaacgaat aattttcttc aaattgactt tgctttgttc tgcttcgact tgaatccctc
     5401 tgcgaatcgt tcgaaaatag ggatcttcca actcatctac ttcagaaact gtcgttatta
     5461 atgcaatatt tgttaaattt tttgcaacgg gacttttttt gacataatga taaaaattaa
     5521 ccgattctgc aatctttcgt ttggtttcat cggttactga aagtgtcgga tcctcattta
     5581 gcacgcgcga tactgttgca atggacacac ccgcccgttt tgcaacatcc ctaattccta
     5641 ccataatagc tcctcttttc atatttgaaa gtgtttacta acatataatc atacattttg
     5701 aaaataagca aatttcgtta aaaataggca ctgtaatcat aattaaaaac aagaaaaaat
     5761 atccgaacaa tttagagtaa ataatgctaa ttctagtgaa tttgcgacga ggaactgcta
     5821 gatcacagtt cattctctct atcgttcgga tattttattg ttaactatac ttatttcaca
     5881 gagctttttt atttgatttt gtgaacagag tctcgagaaa tcaattctcc ctctaatagt
     5941 agtacttgat tttttatttc ttcttgtttg gccagtccaa tcagttggtg aaaagcaatc
     6001 gcccccttgt tacctggatt tagtcgtatt gttgtcaacg caggatccat taattctgca
     6061 aaagtaatat catcaaatcc aataatcgat aattgttcca tcactcgttt attcttattt
     6121 tttaaaaatt ttactaattt agccgctaaa taatctccag taacaacaat tgcagtaaat
     6181 tcagctaatt cttgataaat tcgctcaaat tcagagttgt tatatatcca atgcgattta
     6241 aaggttaaat gatatttctt taatgtatcg atataagctt gatgtcgttc ttgaataaca
     6301 cttggttcac ctacaatgta gtcaaaagaa agaaacgcta ttttctcatg accataatga
     6361 atcaaatgtt ccactgccaa tgtactagct tggtaatcat ttgtatttac aaataaacga
     6421 ttcatttgtt gttcaagttc tcttaatgtc tcttttgaaa gatgcgtatc aatataaaca
     6481 acaggaccat caaaaacgtt atttaattcc aaaaattctt ttacacgtac accaacaaca
     6541 atgaacccct caaaacgcca atttctctga atagttttaa catcttctgc tgaattaaca
     6601 ttatgcacca atacaaaata accatgattc tttgcctgtt cctcaattcc agacaaaact
     6661 tctgcggtaa atggatcaga aaatacaaaa tcagcattat caatagccga aaaataaagc
     6721 aatcctatta aaaatgcaat cgtcttaccg attccctgac agacagaaca cattccacta
     6781 ggatcattaa atgaataagc atttgcatat cctaaactag gtgttgcaaa acatgaaaac
     6841 aacacacgaa ataacgcatt gatgttagta atcgtcccta acgttgagcg cgcactgcca
     6901 ccaatcttct tttgattgac cacaatcgcc gtagaaagat tatcaatctc atcaacctct
     6961 ggacgactgt atttaggtaa aaataaacgg gtaaaactat cataggtgtc attcagttga
     7021 cgaccagctt cttgggtaat agtatcaaaa acaatcgatg attttcctga accagatacg
     7081 ctggtaaaaa tactaatttt atttttggga attttgagac tgacattttt aagttgtttc
     7141 tcttgcgtgg ataatttcaa tatactcgtt catttggtac tcccctttat tttgaaatag
     7201 ttgagaatgt ttcaatgaga cacggtattt ttggcagatg tttagtttac ctagaaagag
     7261 gtataaaaat caagaaaaaa agagaggctg actcataagt agttttatta gtcgaaaatg
     7321 atagtttata aaaataggaa ccgcatcaaa tcaacattct ttagaatgga tttgatgcgt
     7381 ttttctttat tttttgactt atgagtcaac ctcttactct tcgtcatgga tttgattagc
     7441 taattattgc tccacttgtt cacgaatgaa atttaagtac tgcggtaaaa ataaaggaat
     7501 ttcttcaaag cgtggacttt gattttcata cgcttttgca tctaggtttc cttcccctgt
     7561 agcaatttca atcaataatt gatttgggtc acgataataa agtgatacaa aaaattcacg
     7621 atcttttatt cctgaattta taaaattgcg ctcttgtaat tgtttgtcga tgcgttgtaa
     7681 atcagattta ttttctacgc caaacgcaac atgatggacg ctaccaatcc cttcaatggc
     7741 aactggattc tcacgatctt gaataatctg aacttcttga tagaactgag tgtcttgatt
     7801 ttctaagatt gtcactcgtt gatgacccga aaagaaactg acgtattctt tgtgctacca
     7861 gcctaccgac aataactctc gttcagtcgc atcggaatat tgtacgcgta gttgaatggc
     7921 atcaattcct aaaatggcat gctctgtagg gatctggtct gtgacatacg ggaaataatc
     7981 atcaatatct tcaaattcgc gtaagggaac taaagctaat cgtgttgcat ctggcccttc
     8041 aaaacgtaag ataggtcgtc cagaaaatga ttcgattcca taatggcaaa cgccttgtgt
     8101 ttcaaaatat atttcccaaa aatgcaatga tgcttcggaa ggaactttta gtatcgttcg
     8161 ttcaatcgta ttggttccaa aaaactggtt gttcccttct tgtacttcaa aaaaagtcag
     8221 ttcagttccc acacggtggt gattatctga aaaaaacagg tgatacatgg tatgatcgtc
     8281 ttgattaatt gttttcatta gtaattttaa accgagtata ttatgataaa agtcaaatgt
     8341 cggtcgaata aagcgattca caatagaaat gtgatgaata cgtgcgggca taatatctaa
     8401 aaatgagaat caaacctata aaagatggtg aacaactttt gttttcatca gaagaaaata
     8461 agccccttta tttagactat gtaaatcaca atttgaaaat catcattaaa gagaataact
     8521 taaaacatat cacacttcat ggttttcgtc atacccattg tagtctgctc tttgaatctg
     8581 gtgcgtctgt caaagaagtg tatgttcgac ttggacatac agatattaaa accactatgg
     8641 atgatatata cacccatatc gcgaaaaagc agacagagga aacggcaatt cgttttgcag
     8701 atttcatgag ttctacagag gaaaagcaag gaagtttgca gaaggtcaaa agtatggtca
     8761 aaaacgaaaa aagcagacta tccctattag atagtctgct aaacgttgag ttatcagtat
     8821 ttttcttatt ccgcagcgtt tgcgtttttg agaccgtatt gattctcttt ttttaatttc
     8881 atccttttcc aacctaaaaa tgttatttaa ttacttagtt tatataacac gaacctatac
     8941 caagtaccag taaccccagc ttttattccg cgaactttat gaaataaaat cgttctagat
     9001 aaaaatagct tttaatcttt gtctttactg tttataaaaa tttttaaatt atacacctag
     9061 tttaaatcca cgaagtgatg ctcctatttc atcttcagtt ataccaatgt acctttttgt
     9121 aattgcttgc gagctatgat taaaaataaa cattaaggta gcaatgtctc tagttttctt
     9181 gtagtagtga tacccaaaag tttttcttaa tgtatgtgtt cctatatcat ctcgtcctaa
     9241 tttttctgct atatctgcat atatctgata gacacgatcc cttgttatat gtcgaccttg
     9301 ttgccgactc ggaaataacc aatcattttc ttctttacct tcagtatata ataaaatttc
     9361 ttctctcaaa ttatctataa aaatcggttt tcgcttacct gttttatgtt caacaataat
     9421 aggcttactt ttgcctcgta tatctccaac ttttaatcct ataatatctg acatacgtaa
     9481 cccattattg attccaaaca aaaataaaaa actatctctt tccggattac ttgttcttct
     9541 tagccaaaac tgaaaatctt gtatctcttc atttgtcctt aacggttgta cattatagct
     9601 cactattgtt tcccttctta aaaata
//
LOCUS       DACDUU010000093         9123 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.093, whole genome shotgun sequence.
ACCESSION   DACDUU010000093 DACDUU010000000
VERSION     DACDUU010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.093"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..2107)
                     /gene="recJ"
                     /locus_tag="IWL30_002524"
     CDS             complement(<1..2107)
                     /gene="recJ"
                     /locus_tag="IWL30_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAP9707240.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLI"
     gene            complement(2249..2683)
                     /locus_tag="IWL30_002525"
     CDS             complement(2249..2683)
                     /locus_tag="IWL30_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAP9707241.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(2693..3481)
                     /locus_tag="IWL30_002526"
     CDS             complement(2693..3481)
                     /locus_tag="IWL30_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9707242.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(3478..4419)
                     /locus_tag="IWL30_002527"
     CDS             complement(3478..4419)
                     /locus_tag="IWL30_002527"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAP9707243.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(4596..5183)
                     /locus_tag="IWL30_002528"
     CDS             complement(4596..5183)
                     /locus_tag="IWL30_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAP9707244.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(5404..6717)
                     /gene="obgE"
                     /locus_tag="IWL30_002529"
     CDS             complement(5404..6717)
                     /gene="obgE"
                     /locus_tag="IWL30_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAP9707245.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7005..8015
                     /gene="gap"
                     /locus_tag="IWL30_002530"
     CDS             7005..8015
                     /gene="gap"
                     /locus_tag="IWL30_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP9707246.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(8100..9035)
                     /locus_tag="IWL30_002531"
     CDS             complement(8100..9035)
                     /locus_tag="IWL30_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9707247.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2510 a         2091 c         1582 g         2940 t
ORIGIN      
        1 aaattaataa tttttcaggg atacgcaaat attgagctat tgcttttatt ttatgacgta
       61 tatcaattcg ttcttgagaa tggatcaatt taaacaattt tgcgtactgt tctcttgtgc
      121 ctaccccatt caaataggcc tcatcaggtg aaatccccat cagataaaca cgattgaatg
      181 aataagaccg aaaaacttct ttcagatccg ctaaatcatc tggacaatcc ataacaacta
      241 gagagtttgg ttttcttttt tcaattattg cctttacaga atctagtcct tcgaaaacaa
      301 tcgcagcttc ctggataatc tcaggcacga atttcaacgt cgaagaatca aatgctaaat
      361 aaaggctacc ttccgacgtt cggctctgtt cacggaaacg ttttgcccgc caatcaaata
      421 cttgcagacc acccacagaa aaatcagata ccatcagctg cggtttctta cgcccgttcc
      481 attcattgat cgaaagcttt cctaccacat ctacctgatt attcagcagt tcttcttctt
      541 gagtaccaaa accaaaagcg accgcatcaa gctgactcga tgcatcggaa aggacaaatt
      601 tcagatgctg ttggtttgct ccgattcttt tgatattttc tgcttggact tgtcgaaata
      661 aaaactgagg caaaggatta tccgtaccaa atggcgccaa tagtttcagc tcatcaattc
      721 gctctactgt tacttcattt ggcagcaata cttcatcaat tctcaactca ggccctctca
      781 tcaagtcaat ttgatgatcg actatatatt ggttcatctt ctcctgtagt atagtgacat
      841 tttcacttgg catcgttaaa ccgactgctg catgatgtcc tccgaagtaa gtaaataaat
      901 ctcgcatctg gtcaagcatt tcaaaaagat tcaaggcttc aatactacgt ccagatcctt
      961 tagctgatcc atcttctttt aaagtaagga caagcgttgg tttacccgtt tcattcatta
     1021 ttttcccagc aacaatgccc aaaacgcctt catgccatcc tcggtttacc aatagatgga
     1081 tatgattctc cttattgacc atcgctaatg cttcttgtgt tatctgctct acgattgctt
     1141 ttctttcttc attgatctca ttcaattttt ttgcttgaac ggatgcttcc tcttcatcaa
     1201 acgtcgcaag taacgagacg gcaggacgtg gatcgcccat ccgaccgatt gcatttagtc
     1261 tgggagcaat cgaaaaaccg atcgttgttt cgtctgcgtc cctcattttt acgccgcttt
     1321 cttcaaatag cgcctgaagt cctatccgtt ctgaatgatg aatggcatcg attcctaatg
     1381 aaacaaggat tcgattctca tcagtcaatg aaactaaatc ggcgatcgtt cctatcgcta
     1441 ctaaatccaa aaattctgca ggcggttctt ccaataatgc agaagctact ttgaatgcta
     1501 ctccgactcc tgccaaatct ggaaatggat agtgccccgc tggatgacga gggtgaacaa
     1561 tcgcataagc gtctggtaaa ttctcaggta gttcgtgatg atctgtaatg atcacgtcta
     1621 caccagcttg ctgagcgtat gctaccgcat catttcctgc taccccgtta tcaacagtga
     1681 tgatcaactg gattccttct tcgatctttt cttggtatac cgattggttt ggtccatacc
     1741 catgctcaaa tcgatttggc aggaatactt ctacttctgc accaagtaat tccagtgttt
     1801 ctttcattac cgttgcactg gtgatcccat ctgcgtcata atcaccgtaa acgaggattt
     1861 tttctccact gattacagct tcttggattc ggctgattgc tttatccata tcgtgtatca
     1921 gatacggatc atgtaattct tgttcctttg gatgtaaaaa tcggttgata tccttttcct
     1981 catggtaacc tctttgccac agtaacttac cgacaaatgg agataatttc agttcacgga
     2041 tcatttggag aaaggattca gaaggctctg tctcgcttaa cttccaatca aaatttgctt
     2101 gtctcacttt ctcactccta acttagttat tatagcaaag gaaatcagca aaaaaaagca
     2161 gaaagaaaga ataaaaacag atcagatccg ctagacagat gttcagcagg acctgatctt
     2221 ttgtacgacc atttatgaaa tatccctatt agtcaaaata attgaagctt tgtttgggcg
     2281 attgagtttt ttgttcttcc ggcgctttca attgactgat ttgtgcggtt ttcgcttgta
     2341 actccgcctg ataagcagct tccaactccg cacgttcgtt tctaaactcg cgttgctgtt
     2401 cttcttttgc ttcttcaatt ttcttttgaa gttctgtttg atattctgac atttcctgag
     2461 tcagctgttt gatttgcttt ttttgctgcc atagtgttgc cgaagcagtc aaaaatacaa
     2521 tcagtgcacc aatcagcgca gaaccgatga tcactaagat caatggcgca gaaaaggaag
     2581 tgaagccaaa acttacagga acatttttat tgttcaatac agcaaaaaga acaataatca
     2641 aaactaaaac aaatccaaga atcacattgc tttgcttttt caattccttc acctactttt
     2701 tgttaaaaat tccgctggct aaataatcac caatcgttgg aaacagcgta taaaattttg
     2761 atgcaacttc taaaataaac ggttgattga tttcccgttt tggatgtctc atattgcgga
     2821 cgatcatttt tgccaacttg gcaggttcta aaacgatttg tccgactttt tccaaatagc
     2881 ttccgcttgg atcagcctta tcgaagaaat ttgtctcgat tggacctgga ttcactgtcg
     2941 taacagctac gccaagtggt ttcaattcta atcgcaaagc gttggagaag ccaaggactg
     3001 caaatttcgt ggcagaataa accgttgatt ttgcagttgc catttttcca gccatagagg
     3061 cgatattaat gatatgacct tgtcttcttt ctgccatatc gatcgcaaat ttttgtgtca
     3121 aaaccatcat tcctaagatg ttgacttcaa acatatcgta tgcttttcca aggtcgaatg
     3181 taaggaagtc ttcgaaaatc ccaaaacccg cattgtttac cagaatatcg acgcgcccga
     3241 cttcttccat caccttttca tataattcgt ccacgctttc aggattgctg acgtctacct
     3301 tgaaagcata agcctctttg ccgctcaagc gtctgcactc gtctctgaca ccttctacaa
     3361 atgccattct tcgtgcgcaa cttataacga ttgctccttg ctttgctgct tcataacaga
     3421 tttgtgcgcc cagaccgcct gaactacctg ttactaaaac aactttatct tctaaattca
     3481 tgcctcttcc tcctcgcgaa atggaatctc aatgctatca aaatctttca taatcgtcgt
     3541 gttttcaaac acttcttgtg cttctttttc aagctctaaa gctgcttttc ctaaatatct
     3601 cgcactgata tgagtaagta gcagccgttt tgcctgcgct tcttttgcaa cttcagcagc
     3661 ttgatgagtc gtggaatgga aatagttgtg cgccattctt gcttcatctt tattaaatgt
     3721 gctttcatgt accagaatat cgctattttc cgcaagcaca acggaattat gtgtttttct
     3781 cgtatcgcct aaaatagtca cgattcgtcc ttttttatct ggacctacga aatcttttcc
     3841 attgatcgtc cgcccatctt ctagttggat cgtttcacct tgcttcagtt tgccgtacag
     3901 tggtccagct ggaataccta attcttttag acgatccacc tgcaattctc ctttatgatc
     3961 atgttcaacc acacgataac caaaactgtc gatcccatga tttagttttt ttgtataaac
     4021 agaaaattgc tgatcagtaa aaatcggggc tgtttcattt agctcaatga atttcagcgg
     4081 ataacttaaa cgcgtttgcg aaatacctaa agagactttt atgtattcct ctatcccttt
     4141 tggcccgtaa atctctaatg gcgtatcccc accttgaaat gaccggctgc tgatgagccc
     4201 gggaagtcca aaaatatgat cgccgtgtaa atgtgtaatg aaaattttac cgatttttct
     4261 cggacgaata gtcgttcgca aaatctgcat ttgcgtgcct tcgccacagt cgaacaacca
     4321 aacttcattg cgctcgtcta aaagtttcaa tgctatactt gtcacatttc tatgttttgc
     4381 aggaacacct gcacctgttc ctaaaaattg taattccata atctacctga ctttctttaa
     4441 gttcctcttt aattttaggg taaaaagaag ggataatcaa ccgtaagtcg agaatgggtt
     4501 caaattaaaa ataacgttct aaagcgatag cgttttcttt gaaaattact ataaaaacag
     4561 cagcgaaccc gtttgagatc ctctgctgtt ttttattaat ttgaagatcc agaactttca
     4621 ttgtcgttgg aagtttgcgg atgtttgtcc ttccaagtct tttgtgcagc ttctttcgca
     4681 tgattttcag cttctttcgc atcatgcaca tctttttcga catcttcata atccaagcga
     4741 gtaatttctc cgcctaattc catcattacc aattgattca gtgggcgatt gtcctcttca
     4801 tcagcaatca agatcagtcc tgtagctcct tcaccgattt tatttcctac aaagtcgaac
     4861 acggtctggg catcttttat ttctttggca tcttgtgtcg caccgatcat tcctccggca
     4921 aaccatccta ataaaatccc tagcggacca cctaatacac caaccaacat tccgattaat
     4981 ccacccgtag aagatttgtt attaccggta aagtcgataa aatcttctat tttgaattga
     5041 tgtgtcccat taggatcatg gctgacaact gccatctgtt ctcctttaat cgaacgaaca
     5101 gcatgtaact ttttgatttc tgaaaatgcc tgatatgcct ggctttctac ttgaaaattt
     5161 aaaatgatta ctcgtttgct cattaaaaac gcccctttca taccttcttg ttatttattt
     5221 tacctttaat tgaaaaataa gaaaagaaaa gcactctttt tttccaataa aatagggctg
     5281 tgacaaaagt cgtatcacag ctcctttttc cgaataaacg gcgttcttga agctgatcac
     5341 ttctgtcaca acctccctta aatctgaatc tatatttttt ggtatttgtt tttatttgct
     5401 tgcctattcg acaaattcaa attcaaactc accgatacgt acaaggtcgc catctttcgc
     5461 accgcgtgcc cgaagagctt catccacacc cataccacgt aattgacgag caaaacgcat
     5521 cactgtttca tcatgatcga aattcgtcat ttggaataat ttttcgatct tctcaccaga
     5581 taagatccac gttgcatctg aatcacgatc gatttggaac tcaggacctt ctggattgaa
     5641 tccatagtgt acagtttctt cttccagttc ttcttcgtac aatgggaatt ctggcgttac
     5701 ttctaacaaa tctgctgtag cattcaataa cgcgtccaat ccttgtcgag tcactccaga
     5761 aattggaaat actgggatgt catcagcaaa ttcatcttct ttttccttgt tcagctgttc
     5821 tttgaatttc actagatttt ccggtgcatc cggcatatcc attttgtttg ccacgatgat
     5881 ttgcggacgc tctagcaagc gtaagttata agtagataat tctttgttga tggcaagata
     5941 gtcttcataa ggatctcgtc cttccatccc actcatatcg atcacatgta aaattactcg
     6001 tgttctttcg atatgacgaa ggaattgtgt acccagccca acaccttgcg aagctccttc
     6061 aatcaagcct ggcaagtctg ctgcggcaaa acttctgcca tcacttgtag taaccattcc
     6121 tagattaggt acgagcgtcg tgaaatgata agcgccgatt tttggacgag cagatgaaat
     6181 aacggatagt aaagtcgatt tcccgacaga tggaaaacct acaagtccaa cgtctgccaa
     6241 tactttcaat tctagttcga tttttcgctc ttgtcctggt tcaccgtttt cagcaatttc
     6301 cggtgcagga ttccgaggag aggcaaaacg gatattccct cgtccaccgc gtccaccttt
     6361 tgccacaact agtgtttgac cattttcaat taaatctcct aaaagagcac cagtctccgc
     6421 atctcggaca gtggttcctt gtggtacttt aacataggta tcttctgaac cacgaccgtg
     6481 catcccttta ctcatcccat tttcacctgg ctgcgctttg aaatggcgat tgaaccgaaa
     6541 atccatcaat gtacgtaacc cttcatcgac gatcaagatg acatcgccgc cacgaccacc
     6601 gtctccgcca gccggtccgc cgtcaggaac gtatttttcg cgacgaaatg caaccattcc
     6661 gtctccgccc ttgccagctt tcacatcgat cgttacttga tctaaaaaca tggacataat
     6721 taagtcctcc tattattcat ttatttattt gactgcctga atttccacaa taaacaaaag
     6781 tcttacttat tttatcggtg aaatctgatt ttgtctatca tttcagcaaa aagattgttt
     6841 accttcttct ttttttatca tttggtaagg tttccaacct tttttctgct accaagtata
     6901 attttttgac aaaatattct ttgcgtagca gaataacagt gtattaattg tattaattga
     6961 ggatgccgtt tcacagcacc cctaataaag gaggaacttt tgatatgaca gtaaaagtag
     7021 gtattaacgg atttgggcgt attggccgtc tggctttccg ccgcatcaaa gaagtctcag
     7081 atgatattga agtagtagca atcaacgact taacaagtcc aactatgtta gcacaattat
     7141 tgcaatttga ctctactcat ggtacttatc ctggcacagt tactgcaaca gaagactcaa
     7201 tcgtagtgga tggcgaagca actcgtgttt atgcagaacc cgacgcaagc aaaatccctt
     7261 gggcaaaaga aaacggtgta gatatcgtac tagaatgtac tggtttttat acatctgaag
     7321 aaaaagcaaa agcgcattta gatgctggtg tcaaacgagt agttatttct gccccagctg
     7381 gtgctatgaa aaccatcgtc tataacgtca atgatgatac attagatgaa aacgacaaaa
     7441 tcatctcagc aggttcatgt acaacaaact gtttagcacc tatggcttat ttcttaaata
     7501 atgaatttgg gatcgaagtt ggtacaatga caacagttca tgcctacact tccacacaaa
     7561 tgctcttaga tggacctgta aaaggcggta atttgcgtgc tgctcgttca gctgctgaca
     7621 atactatccc acattctact ggtgctgcga aagcaatcgg cttagttatc cctgagctac
     7681 aaggaaaatt acaaggacac gcacaacgtg ttccagtcgt tgatggttca ttaactgaat
     7741 tggtttcagt attgaaaaca aaagtaactg ctgatcaagt aaacgaagca atcaaaaaac
     7801 acacagttga caatccttca ttcgggtatg atgaccgtca aatcgtttct agcgatgtca
     7861 ttggaacaac tcaaggttcg atctttgacc caacgcaaac agaagtaaca actgctggtg
     7921 atttccaact tgttaaaact gttgcatggt atgacaatga gtatggattt acttgccaaa
     7981 tgattcgctt actagaaaaa ttcgctaact tgtaatttga tagatcaatc gataaaagaa
     8041 gcctttgact ggatccagtc aaaggcttct ttcttttcat cccctacttt taaactcgtt
     8101 caatgaacat attggcttaa aaattgttgg actacttgct gatatttttc aggatctgtc
     8161 ttgtatgctt ccgcatgctc ggctcctttt acaacatact tttcttttgg tcccttcgtt
     8221 gcacgataga ccttatctaa catttcaaat ggtacaaatg tatcttcgtc tccatgtata
     8281 aagagcattg ggcgctggtt tttcttcaat tgttccactg catttgcttc accgaaaaag
     8341 tagccagcac ggacttttgt cactagactc gttacttgca taagcggaaa tgctggaaga
     8401 ctaaacatat cgtctagctg ataagcaagc tcatcatgaa cggaagaata accgcaatct
     8461 tcaatgattg ctttcacatt atctggtagt ttttctccgc tagtcatcat aactgttgct
     8521 gctcccatgc tgacaccgta tagaacgatt tcttgcgatt ttccattgtt ttctagcact
     8581 ttgtttatcc actgtacata gtctttacgc tctggccatc caaatccaat gtaatcccct
     8641 tcgctttttc catgtcctct tgcatcagga acaaggacat tgtaacccat atcatgaaac
     8701 atctttgcgt aattggtcat cgtttcagcg tttcccatat agccatgagc cacaagagct
     8761 gttttttcac ttttcgtttc agcaggaaga taaatcgctg ataatttcag accgtctttt
     8821 gaagtgatgg tccaatcgct tcggttttct tttgaactaa accatttatg tgcatccgtt
     8881 tctttggatg tctgtgttgt accaggtgta tcttcgtcta aaaagtcttt ctcagaaggc
     8941 acaacagcat aattataaaa ataattccct gcaaacacta acgcaacaat cagagctaaa
     9001 gcgacgccta aaaggatttt catccaaagt ttcataaatc atccatctca tttctctttt
     9061 ttcatataac cttgaattag tataatcaaa gaatctctac tatgcaatgt tcatttttat
     9121 aac
//
LOCUS       DACDUU010000094         9058 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.094, whole genome shotgun sequence.
ACCESSION   DACDUU010000094 DACDUU010000000
VERSION     DACDUU010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.094"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..106
                     /locus_tag="IWL30_002532"
     CDS             <1..106
                     /locus_tag="IWL30_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9707248.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            583..783
                     /locus_tag="IWL30_002533"
                     /pseudo
     CDS             583..783
                     /locus_tag="IWL30_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1495..2100)
                     /locus_tag="IWL30_002534"
     CDS             complement(1495..2100)
                     /locus_tag="IWL30_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9707249.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2282..2617
                     /locus_tag="IWL30_002535"
                     /pseudo
     CDS             2282..2617
                     /locus_tag="IWL30_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2782..3009
                     /locus_tag="IWL30_002536"
                     /pseudo
     CDS             2782..3009
                     /locus_tag="IWL30_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3135..4106
                     /locus_tag="IWL30_002537"
     CDS             3135..4106
                     /locus_tag="IWL30_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9707250.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4246..5559
                     /locus_tag="IWL30_002538"
     CDS             4246..5559
                     /locus_tag="IWL30_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP9707251.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5602..6468
                     /locus_tag="IWL30_002539"
     CDS             5602..6468
                     /locus_tag="IWL30_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9707252.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6465..7313
                     /locus_tag="IWL30_002540"
     CDS             6465..7313
                     /locus_tag="IWL30_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006639650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9707253.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7327..8799
                     /locus_tag="IWL30_002541"
     CDS             7327..8799
                     /locus_tag="IWL30_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAP9707254.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            8816..>9058
                     /locus_tag="IWL30_002542"
     CDS             8816..>9058
                     /locus_tag="IWL30_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9707255.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         2871 a         1533 c         1849 g         2805 t
ORIGIN      
        1 cgcatctaaa cgaaataata ttcctagaaa atcactggat tatcgaacac ctttggaagt
       61 atttttgagt tacgtaagta ttgatgatct gtctaactta atttgacaat taagacattt
      121 aaaaagaaaa gtgacagata ggaataaaaa cttattaaat gtagttatga tctgttatat
      181 ttcatttcta atactaggtc ttccacaagc agtgtttcct gaaatgtttt ctggagactg
      241 gggtattgtc agctattcta tattaacatt gattgctatg gttttaggta cttcatttta
      301 taatgctttt gtaagtaagc atgttgagga atatgaagta ttaatgtact taaaagagaa
      361 aaaaacaagt aattaatatg agaacaccac gcatagtttt tattttgaga tattctagaa
      421 tgaaacacat taagaacgaa agcaaagcaa aagacgccag aacttctact accttctaag
      481 ttttttcata tttgtttgtt atgctataca tagatctgca taattagact tgcgctgccc
      541 gttattgaag tttcaggcag cgctcttttt gggggatcga taatggaaga agaaaaaata
      601 tttgagaaac gctggcagct agcatcaagt gaacaaagag caagatacaa taatttgatc
      661 acctcctacc ccactgttga ctagacatat aaggaaaaga aatacttact ttggctgtgc
      721 cagttagata tagatacctt tgaaacattt gaagtgatcc tagataaaat taaacagaat
      781 tgatcagaaa caagcgaagc cttctctcgg ttcgcttgtt ttgctattta ttagttggct
      841 cagggcaact ctaagctcgt ttgaaagaaa accgatggat aatccccttg tttgtaccac
      901 tttagaatac atggatgtac gtattgccat tgactcctat tagttactga aaaaaaattt
      961 aaacaagttg agtattgcag ataaatagct ccagtgaggt gaataactgc tccactcaag
     1021 gcaatagata gctccatcca gggcaacaaa ctgctccact ttcatattta atagatgaat
     1081 aaccttctgt ataatagaaa ggcatacctt tcggtatgac aacttataca gaatgttatt
     1141 ttattggttc actatcgaaa aattttggag ctttattttt ccggtatttc tcaacgaacg
     1201 attagtttaa ctgtgggaag ctccagacat acgattcgag aagttacttc tcgagcagac
     1261 aggaaggggg tgactgaact aaccgctgat atgagcgata cttggctgta ggaatatctt
     1321 tttccggaaa aacaaacaac agcacgaggt taccttcctg tagactggga atatgttcat
     1381 aaagaactaa tgaaaaagaa tatgacattg aaactccttc atagagaata tggggtacat
     1441 ggaacataga aacgaaaatg cacggtagaa aagcgaatga atatagtata aattctacaa
     1501 atcattcgct ttctttattt tatatatggt tggtcgagag agaccatttt ctttggcaat
     1561 ttctgcaact ggaactcctt gatttaacat gtccaccaca gttttaaaga ctaatcgttt
     1621 ttgcggatct gttgagttct cagaatattc aattggtcgc cccttatatc gaccagcttt
     1681 tttggccaat aatactcctt gccgctgacg ttcacggatt ttctcacgtt cgttttgtgc
     1741 aatataactt agcaatgata aaaacatatc gaacatagca gtatctagga gttcatttcc
     1801 agtattaaaa tctaaaaact gtgcatctag tatttttaat gctacttttt tctgtttcat
     1861 aaaggtaact gtatttttta tgtcttcata atctctgcct aggcgatcca gtgatgcaac
     1921 aatgacacaa tcaccttctc ggatataatt tagtaatttt tgaaactcag gtcgctggat
     1981 atctttacca ctggcttttt cttggaataa tttatcaatg ttaaggtctt ccatcattgc
     2041 caattgtcga tctaaatttt gatcttgtga agacactcta atatatccaa tttttgccat
     2101 aaaaactcct tttaaaaaat gatataattt acatgtaaaa tttacttgta gttttactgt
     2161 tatcatagca tttttaaaaa taaattttaa gtctacttat aatttacttt ggaggttttg
     2221 aatgaatgaa attaagcgca ataaaaattc ttttggtgtt tatttaggag agcctagctc
     2281 ggaacagtta aataaaggtg aagctcgtca tgctctatgt cgaaatatat tttatggtag
     2341 aaaaggcaaa ctttatcaaa cttattacga cggtatggag gaacagctca acgcattagg
     2401 attagtaact aatgctatca tatattggaa ttcactatat ttagaaaaag tctttgaaca
     2461 aattaaaacg gaagggttcg actgttccga agaagtgtta cgcaagcaat ttccattaat
     2521 tagtgatcac attaattttg ttggcaaata tagtttccgc tatgaaaaag aattatcgaa
     2581 tggaatacta cgtccgttaa atatagagaa ttattgagaa tgttcgtctt ttggttccta
     2641 ccgtgcattt tcgcacggat gttccagata ctatttttga atgtataaaa ttgaaaacac
     2701 aagaatacta ataaatcaac gattcttgtg tttttaattt ttcactaccg tgcattttcg
     2761 tttctatgtt ccatgtaccc caatcttgaa aaattaatta aagacttaga taaagccatt
     2821 gacgacttag tcgtggtgct accggagtat caatgtttaa ccagtatccc aggtattggt
     2881 cctgtttagg ctgctggaat actcgctgaa attggtccaa ttgaacgttt ttctgatgaa
     2941 ataaaattga caaagtatgc cggcctatat tggcgacaga gtcaatccgg taaatctgaa
     3001 tacgaaaatt tttttacgaa aagcttcacg tctaatatct attttaaaat aaaaatattc
     3061 ctgctaattt ggaatcgttt ccttcttttg gtataataat acctgaaatt cttttgagag
     3121 gagttgttcg tgaagtgaaa actactatga aggatgttgc tgcgttagca ggagttggtg
     3181 tgggtacggt atctagagta ataaatggag taaaggtgaa agaggttacc aaagaaaaag
     3241 taatggctgc aattagtgag cttaattatg agcctgatga atatgccaga ggtatgaaaa
     3301 cgaatagtag caatacaata gctcttattt tacctacgat ttggcatccc ttcttttccg
     3361 agtttgccta ctatgtagag gaagctctca gccaaaaaaa ttataagctt tttttgtgta
     3421 atgcgaacgg cagtgcggaa aaagaaaaag aatatatcca aatggtcaag cagagtaaag
     3481 tagacggaat tataggtatt acctattcag acattgataa atatgtatca tcaaatttac
     3541 catttgtcag tatagaccgc catttttcgg aacaggtttc ttatgtaacg gcagataatt
     3601 atttaggtgg gcaaattgcc gctcaagaac tacttactcg taactgtaaa aacttgtgct
     3661 atgttggggg tgtttcacct taccctaacg aaacaaacaa tcgtaaagct ggtttttaca
     3721 atatttgtga ggaaaataaa atagctgtga aggtattgga tatgcctgaa ccaattagca
     3781 atttagagga acaattgctc gctttttttt cggataatcc tgaaattgat agcattttta
     3841 ccatcaatga tttcatggct ttggatgtga taaaagccca aaaaaaacta gggaaggaag
     3901 cacctaagga ttttcaaatt attggttttg atggagtcaa gctatcgttg caaaaagact
     3961 atttactatc aacgattgta cagccgatag aggaaatggc caaggtttca gtagaaacac
     4021 tcatggcgat gattttaaat gaagaaccgc ctaagcgtgt ggtagtaccg gttttctttc
     4081 acgctggaga gacgactaaa gagtaaaaaa ccggtttata acgatttttt tgagataggt
     4141 tgacagaaag tgatttcaat gatatcttaa aaccaattaa ttggaaacgt taccaattaa
     4201 ttgaaaaata aaacggaaac gtttcaaaaa ataggaggaa catcgatgaa aaagagaatg
     4261 ttagcaattg cagcaatttt agcattaaca gctggaggac ttgcaggatg tggcagtaag
     4321 gagaaaagtt caggtagcag tgatccagga gaaattaaga tttgggttca gttttctgat
     4381 gaaacgcctg aaggaaaagc ttgggcagag attgtgaaca attttaatga tgaatacgag
     4441 ggtgaatata aagctgttac tgagtatatt cctcgtagcg gaagcggcgg tggatatgag
     4501 gataaagtaa atgctgcaat tacaaccaat acccttcctg atgttattac gttagatgga
     4561 ccgaatactg cagcttatgc tcattcagga gtcattgctc cattagatga ttatttgaaa
     4621 gatgcggaca tggatgatgt attagacagc attattcagc aaggaaccta cgatggcaaa
     4681 atgtatgcgt ttggtttctc agagtccagt gtgggtgtct attacaataa gtcgctgttc
     4741 aaagatgctg gaattacaga agatcaattg ccgacgttag aagatccttg gacttggacg
     4801 gaatttcaag caatttgcca aaagctgact gataaataca aaacgcctgc aattgatatg
     4861 caaatggcgt cgggagacga aatgctgacc tacgcatata caccatttat atggtcgaat
     4921 ggcggcgatg tcgttaatgc tgatggaacc aaagccgaag actattttaa cggtaaaaaa
     4981 tccgttgaag cgttggaatt tattcaagaa ttggttgaaa aaggttacac cacaatcagt
     5041 cctattgaaa aaggatttga aactggcaaa tatgccatga tgctgagcgg ttcttggaca
     5101 atggcagatt tagaggaaaa ctatcaagag attgattatg gtattttgcc ttaccccgtt
     5161 tcagatgcaa ctggggagtt agtttcacca accggaagct ggcagttagc aatgactgag
     5221 aaaacagaga aaaaggatgc ttctgcggcc tttattaagt ttgcaaccag tacagaatct
     5281 agtcgtatta tgagtttagg gaacagtgtt ctacctatca gaaattcaac agttgattta
     5341 attaaagatg aagtgtcgga aggaatgcag gtattaatta agcaaaacca actttctgga
     5401 catgcccgcc cagtggttgt agcttatccg caagtatcta gagcattcca acaagcagtt
     5461 caagatatta gctacttcga ggaaaaccca gatgtacaaa aagtagcaga tagtcgcgct
     5521 gctgaaatgc aacaggcaat tgatacttca ctaaaataga agtttctaaa gggggccaat
     5581 caagtgttaa aaaaaccgac aatgaaagac aatttgatag gttacggctt tctagcgcca
     5641 gctttgctgc tgctgacgat ttttctgatc atacccgtaa tcatggtaat ttattatgcc
     5701 tttactgatt attatttgtt aacgccagat gctagacaat ttatcggctt agaaaatttt
     5761 aagcgactgt tcgctgatcc aatttttgta aaaagtattt ggaacacggc taaatttgtt
     5821 gtttggatta ttccggtcca aataggggca gctttaggaa tggcacttat tatcaacaaa
     5881 aatcgtaaag gaaatatctt ttttaaagta gccttttttg cgccagtcgt gatgtcctta
     5941 gtcgttattt cgattttatg gttgtacttg ttaaatccta atgaaggtct ggttaatacg
     6001 ctatttgcaa agattggaat tgcttcgcag ccttttttga ctagtcccaa acaagcaatg
     6061 tatacgcttg ttttcgtatc ggcatggcaa ggtgcaggat atcagatgct gctgttttta
     6121 gggggaatgc aaaatattcc gcaagacgtt tatgaggcag ccgaaattga tggtttttct
     6181 aagtttcagc aatttcgcta tatcaccatg ccattattaa agcccacggc gatttttatt
     6241 ttacttacca cattgatttc ggcctttaaa ctaatcattc agccaatggt aatgactcaa
     6301 ggtggaccaa tgaattctac tatgacaatg gtttattaca tttaccaaac tggatttaca
     6361 gaccgtatgg taggctactc cagctccatt gcgttaatct ttaccactat gattggttta
     6421 attacaattg ctcaaagaaa attggtaaag gaggatgatc cagcatgacc aagaaatttg
     6481 gaccgctaat gatcgtagaa tattgtttgc tgttcctttt agcggcatta ttcattttcc
     6541 cgatgctgtg gatggtagtt tcctctatga agcctgaagc agatgtctat caaaatttag
     6601 cttccttcaa agctttttta cctagtttga atccagctaa ttggtttaaa acttatcaag
     6661 aggttatttc ccggttcagt gtgggaactt atttattaaa cagtattttt tatggtttaa
     6721 cctttgcctg tgggtcaatc attgtaaact ctttggcggg ttttgccttt gcgaaggtta
     6781 atttttcagg caagaaaatt atctttggag ctttgttagc tctgctgatt gtcccgatgg
     6841 agacggtttt aattccgcaa tttaccatcg ttaattcact aggcttagtg aatactcgct
     6901 tggcagtgat tttaccagcg atggccagcg cgtttaatat ctatctgttt agaaatttct
     6961 ttatcgcgat tccagaagaa atcattgaat cagcgaaatt agatggtgca aatattgtta
     7021 aaattttctt tcacatcatg ctgccaatgt ctaaaccagc catagccaca gtaggtgtct
     7081 tatcctttat ctccagctgg aatgattata tttggccgtt aatggtctta actgacagca
     7141 gcaaattttc aatgcaagtg gcaattacta cgatcaatac tacacagcca gtttatatca
     7201 atcaagtgat ggctgtttta accattgcaa cgattccact gattattgtt tatatcgttg
     7261 cacaaaaata tattctgcag ggacttggcg gttctggaac gggtataaaa taggagtgaa
     7321 tagtgaatgt tgcaaaaact tttaaaggcc aatgagttta ttgagaaaaa tgctcaaaca
     7381 gtcaatcaag aataccggaa taaatttcat ttgatggctc cgattggctg gatgaatgat
     7441 ccaaatggtt ttgtttatta tcaagaggag tatcacttgt tttatcaata ttatccttac
     7501 gacagcatct ggggaccaat gcattggggc catgctaaaa gcaaggatct aattcactgg
     7561 gagcatttgc cagtagcact ggcaccaggt gaaagctatg acaaggaagg ctgtttttcc
     7621 ggcagtgcca tagaaaagga tggcaagctg tatttgattt atacaggaca caatgttgtg
     7681 gaagggcaag ttcgacaagt tcagtgtttg gcagtttcag aagatggcat tcactttgaa
     7741 aaatatgccg gaaatccaat catcgcagaa gatcacttag cttctgttgc aacgaccgaa
     7801 gatttccgcg atcccaaagt gtttcagcga aaagaagact attttactgt agtcgctact
     7861 aaaactgcag atgctcgtgg acaaattgtg atgttccatt ccaaagattt actaacttgg
     7921 gagttttatt ccgtattgct ggagggaact ccagaacaag gcattgtatg ggaatgccca
     7981 gacttgtttg aactagatgg caaagaagtc ttgattcttt ctcctattca aatgactcca
     8041 caaggcaatg cttaccaaaa tacgaactca actgttgctt tcatcggtac tgtcgattgg
     8101 caaacaggca aatttctagt agagaatttt catgaaatcg atggcggttt agatttttat
     8161 gcaccccaga cctgtgaaaa cgaagcaggc caaagaataa tgattgcttg gatgcaaatg
     8221 tggcagcgta aaatacctac gaatgatctg ggccatggtt ggtcaggttc catgaccttg
     8281 cctagagagc tgagggtggc tgataatcaa ttgttgcaac ggccagtttc atcaatttat
     8341 caagtaattg atccgcagga aaggattgaa tgggcgaaga ctgaagagaa tcgcttctta
     8401 ctgaaggact tagtatatga ccagcactat ttacgcttgg aaatcaattt aaaacaggca
     8461 acgaaactag aacttcacta tgcgaaggga cccgaccata cattactgct taactatgat
     8521 gttgaacggg agtgctttac tgcttccagg gaacacatgc cgtatgattt atcaggagct
     8581 gaaaaggaag tgttgaaaaa gcgcagtgtg acgattcctt tggaaaataa ttgtctaacc
     8641 atcgaaattt tccgtgatac ttcagctatt gagctttttc taaatgatcg tctgactatg
     8701 agtacaactt tttatgagac agaaaaagga caggattttc ttttgctggc agacagtgaa
     8761 gtaacctttt cattggaaag cggcttaatt aatctataga agggagagct cagtgatgat
     8821 ttatggttca atcgaagcag gcggcacaaa atttgtgtgt gccattagcg atgaagaaat
     8881 gacgatcaag gaaagagtga gctttccgac aacgacacct gaggaaacaa tacctttagt
     8941 cattgacttc ttcaaacaat accaagcaga tttagcaggg attgggattg gctcatttgg
     9001 gcctatcgat atccatcgcg attccgccac ttatgggtac attacctcga ccccgaaa
//
LOCUS       DACDUU010000095         8900 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.095, whole genome shotgun sequence.
ACCESSION   DACDUU010000095 DACDUU010000000
VERSION     DACDUU010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.095"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..463
                     /locus_tag="IWL30_002543"
     CDS             <1..463
                     /locus_tag="IWL30_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707256.1"
                     /translation="CLHHLFLSKTIPRYSKNKKAEKFCLLLNKFTLIAPILAFIIFSV
                     LLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFLMSLKPPKSISLCLVINGIFLLSLI
                     IFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYIGYFPNKNNNFYFYRNKL"
     gene            complement(589..1266)
                     /gene="nth"
                     /locus_tag="IWL30_002544"
     CDS             complement(589..1266)
                     /gene="nth"
                     /locus_tag="IWL30_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAP9707257.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1273..1971)
                     /locus_tag="IWL30_002545"
     CDS             complement(1273..1971)
                     /locus_tag="IWL30_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAP9707258.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2239..3423
                     /locus_tag="IWL30_002546"
     CDS             2239..3423
                     /locus_tag="IWL30_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAP9707259.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3429..3872)
                     /locus_tag="IWL30_002547"
     CDS             complement(3429..3872)
                     /locus_tag="IWL30_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9707260.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(4098..4385)
                     /locus_tag="IWL30_002548"
     CDS             complement(4098..4385)
                     /locus_tag="IWL30_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707261.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4556..5908)
                     /locus_tag="IWL30_002549"
     CDS             complement(4556..5908)
                     /locus_tag="IWL30_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAP9707262.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(6181..6627)
                     /locus_tag="IWL30_002550"
     CDS             complement(6181..6627)
                     /locus_tag="IWL30_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP9707263.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6863..7789)
                     /gene="cysK"
                     /locus_tag="IWL30_002551"
     CDS             complement(6863..7789)
                     /gene="cysK"
                     /locus_tag="IWL30_002551"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAP9707264.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(7929..8816)
                     /locus_tag="IWL30_002552"
     CDS             complement(7929..8816)
                     /locus_tag="IWL30_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9707265.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT         2432 a         1810 c         1459 g         3199 t
ORIGIN      
        1 ttgtctgcac catctttttt tatcgaaaac aatacctcgt tattccaaga ataaaaaagc
       61 tgaaaaattt tgtttattat taaataaatt tactttaatt gctcctatcc ttgcatttat
      121 tattttttct gtacttttaa gcactactct taaaggaaaa tttatggaaa gaagctctca
      181 tgcaatgatt ctcacttttt tatggttgtt gttcaccaga atctatattt ttctgatgag
      241 cctgaaaccg cccaaatcca ttagtttatg tttagttata aatggtattt ttttattatc
      301 acttatcatt tttattacac ctttagatag atacgtcact tatttatata atcctttgga
      361 atactggact tattttatag gtatattgga aggcattatt ttctacatcg ggtattttcc
      421 caataaaaat aacaactttt atttttatcg caataaattg taataagtta ccatcctagc
      481 tactgagtat gtacatttac tttttcatgc aaaaaagata aacgaaacag cttttctcca
      541 taataacaag ataattcata catttatttg caattaaaga aaccacgtct acaggcgtgg
      601 tttctttttt agcgcctttt cttttagacg catccgattt ttcccatctt gacatattga
      661 tagcaaagga catacctcgc acttgggatt tcttgccgtg caatgatatc taccaaaaaa
      721 aatcagtgta tgatgtgtct ttacccataa ttcttgtggt actttccgca tcagtgtttc
      781 ttctacttcc atgaccgtag catctagttt gcagatcctc aggcgttttg agacacgttc
      841 cacgtgggta tctactgcga ttgctggtat cccgaatgca tcccccaata cgacatttgc
      901 tgtctttctg cctacaccag gaagagacat caattcttct cttgacgttg gcacttgccc
      961 atcgaaacgt tcaatcagct gctgtgcaca cgctttgatg ttttttgctt tatttcgata
     1021 caaaccaatc gtttttattt taagaatgat ttcatcgatt gaagcttctg ctaacgcatc
     1081 gggtgtcgga aaagaggcaa acaaatctgg cgttgcttta ttcactgata catctgtcgc
     1141 ctgggcactt aagatcactg caatcaataa ctcaaatgga ttattgtgct tgagttctcc
     1201 atgtgcttcg ggaaacattc catacatcgt ttctagagct tccattgttt tttgtttgct
     1261 taacatattc cgctactcct ccaaccaatt tttcagtgag actttcggca attcttcttg
     1321 ttgagctgac tgttctactt ctctttgaag aagctgctgt tttcttcgtt tttggtcttc
     1381 ttccacttgt tgttttgtac gtagattttt tctttcccac gatagcaaaa tgcggtcaac
     1441 gtaattaaaa ctataagcct gatttaatac ggcttcccgc agtgctagct tcaaaatttc
     1501 cggctgataa tggtcttctt ctagccattg tccgatccgc tggaactcga tcgaagaaag
     1561 cgggcgtcca aattcttgtt caaataattg atacactgac tggatttctt tttcttgtgt
     1621 tttttcttcg tatttttttt cttgtgtttt taaatactca cctaatagat catagatggg
     1681 gtaaagatta tatcgatctg cttttttacc gttgacaatc gtcgtttcta tcttgataaa
     1741 tccatttgtt acgagacgat ccagtatttg aaaaatctga tcttgtttga ttcccatatc
     1801 catagcaatc atctgcaaat cagggaaagt atctccttca agctgtgcca tatgcaactg
     1861 tagaacaaat aaaaattctt cagattgtag accgatccta tgataatttt taagtaaaag
     1921 attagatatg actgtttgtc cagcgcttaa gtaattttct aaattcaaca tgatttctcc
     1981 tccgctcaat cacagtatac gtaaaaaaaa catgggaagc aagaacaggt ttcttttcta
     2041 tttacttcac tgatcggtca gatttacaat gtcgtctact tgatttttgt ttgttcatgt
     2101 ttttgatgcg tatcttcact tttcggaata aataaattaa gtagatttcg ctgttcctta
     2161 gaatgttgac aaggaaacaa aattactata cactattttt gtttccaagt taacattatt
     2221 gcataaagga gtttttacat gtacagtttc acaaataaac attctaaagt ttttggatac
     2281 gtctctgtct tattcactgg attaggattt actatcgtca gtcctgtact tccgttttta
     2341 acgctgcctt attctcactc catacatcag caagctttct atatcacttt gctcatgtct
     2401 gtttatgctc tagctgcctt cttatctgcc cccattctcg gtagcttaag tgatcatttt
     2461 gggcgtcatc cgatccttat tatcagttta ttaggatcaa gtatcggata tttgcttttt
     2521 ggtttaggaa attcgatatg gatgttgttc cttggtcgta tcattgaagg attgacggca
     2581 ggagaaatct ccaccttata tgcttacttt gccgacatca cggaacctaa tgaaagaacc
     2641 aaagtttttg gatggatggg tgctttagtc ggtataggaa caacgctggg tcctattata
     2701 gggggtctat tagctgagtt ggggaattct gttcctatat ttattggagc actctttact
     2761 ttcttaaatg ccgtttatgg ctacacattt atgctagaaa gccttccaat aaaaaaacgt
     2821 tcagtagatt taagtttctc ccatgtaaga ccttttcacc aattacaaca attattcaag
     2881 atttcatccg ttattccttt attgacagca ggttttgctg tttggttggc agctggctct
     2941 cttcaatcta tcttttctca attttcgatt gacgcctttc agtggaaggc tgggttggtt
     3001 ggtttaagct tctctttgat tggaatcttg gatactgtct cgcaattgtt cattatgcct
     3061 cgattgctca aaaaattttc agaacagcag atcacaagaa tagggatgtt cagtgaaatc
     3121 ctcgcctatc tattcatcac attatctgga attcttctac tgccgatttt gtttttattt
     3181 gggattatct gttatggttt cggtgattct atttttactc ctgtattcaa tggccaacta
     3241 tccaattctg tctcagataa ccagcaaggt cttgtaatgg gcggtaccca aagtatacaa
     3301 tctctatcgc gcgtcatcgg cccgctaatc gcaggacagc tttatgctgc agcaccattt
     3361 cttccgacgg tattcggatt tttgcttatg atttttgcct attatcacta caataaaaaa
     3421 tagtagcttc atttgctact tcgtataaat tctctgtatc gaccgataaa acgaaatact
     3481 gtgtcaaatt cttcttttga catattttca aatacagtag catctcgctg tacccatttt
     3541 tgatggagtt gctcgtgtct attccttatt ttttgtccga ttggcgtcaa ttgaaaatag
     3601 atttcttttt tattctcttc gctttgataa gtgtttatga tccctctagc tatcagtttt
     3661 tttgttagct tactgatggc tcctctagtc atgtaactag cattcgctaa ttttgtcacg
     3721 ttaggatcag ggattttccc gatatgttca atcacttcta gttcatttag agtaaatcct
     3781 gcaaattctt tttccatttc tggtttatta cgatgaataa tctcattagc taaatccaga
     3841 tattcttcta ttagcagttg tcttttatcc atttctacac cttctttttc tttcattata
     3901 ttgttttttt gtttcctatt caacaaagat aaaaatattt tgtaggaaaa cccaatctga
     3961 aaccaagaaa aaaaggggtt gacaaagctt gtcataaccc ctttttctga ttaaaagata
     4021 gtaaagagac agtcatcggt aattgtgatg attttttgtc atcacttaag gatgagcggc
     4081 cttttcaaaa actgtcttta aagatcatca tcagtttcta gcacatatgg ctgaccttca
     4141 tatgtgtact ctgtattcaa tgtgccgtca ttttcatctg agtagatgcg tctactgaaa
     4201 ggaaaacgat gagaaacatt atctttggcg tcatttgctg gcatcgtgta atattcctct
     4261 tgcgtttcat tcaactgaga gagtacttga tctgccaact catcaatatt gatcgttaat
     4321 ttcttcgaag cacttttata atcttctaaa aatgcatctt gttggacaaa atattcttta
     4381 ttcatcttct ttctcacctt tcttttcaaa atcaaataaa gctgcttgtt cttttatgta
     4441 acagctttat ctttgctgct tattgtgaca aaaaattcga agaaagacaa ctaattgtac
     4501 ttttcacaaa aaaagacaga gggtcgtgac aaaattgtgt cacaaccctt tttctttatt
     4561 gtgcagtttc gatttcgcct agcgctgctt gtgcaagtag gttcaagtaa ttccatgggc
     4621 ggtcaaaatg tggttggaag aagaaatctg aaagtgcgag atcttctaca gtcattttat
     4681 tttgaacagc taaagacatg gtatttgctg attgtgtaat gtcgtatttg gacatgaact
     4741 gagcacctac gatacgattc gttccttttt catagactag ctccatcatt actttttcag
     4801 tagttggcat gaactctgga cgataattat cttcaaatac agtcgcttgg acttctagat
     4861 cgttcaaagt tgcgctttct ttggtcacac ctgtcgaacc gatcgtccag ccaaataggt
     4921 aaagtcctga agttccttga gtaccacgat acgccatttt atgatctgtc aagttatttc
     4981 ccacaagcaa accttgacga acggcatttg ttgccaaagg aatatagttt tgcgtgccac
     5041 ttggattgta gttgactact gcagagtctc cagctgcaaa aatgtctgga ttacttgtct
     5101 gcatgtattc attcactttg attgccccgt ttggcagtgt atctactttt ccttcgatca
     5161 gttttgtatt tggacggaag cccacacata aaatgaccat atctgtatca aatgtatctg
     5221 ttggtgtagc tacttgtttc acttttcctt cttcatctgt gataaattca gtaacatttt
     5281 cgcctagcgc aagagtaaca ccgcggtctg tcaattcttt ttctaagata tctgtgaatg
     5341 gtttatctag atatttattc aagatgcgat ctaatccatc aatcaatgtc acttgttttc
     5401 cagattcgac aaaagcttct actaattcaa taccgatata tccaccacca acaattacta
     5461 cacgttttgc ttctttcgct tgtgcaataa tttcatttgc ttgattgtag tttttgcata
     5521 ggagtacatt tttcgcatcg attccactaa tcggtgggat aataggccat gaaccagttg
     5581 tcatcactaa tttatcataa gaaaccgttt ctgtttctcc tgttttcaga tcttttgccg
     5641 ttactgtttt attttctgta tcgacgtctg ttacatcgtg ttccatttta actgttgctc
     5701 ctaatgaaga taattcttca ggatttgagt agaacaagcc tgctgggtcc tttactactc
     5761 cgccgacata aagtgcgata ccgcaagata agaaagaaat attgtcattt ctttcatata
     5821 ctgtcacttc tgcttctgga tgatttttta aaatactttt tacagctgca gtacctgcat
     5881 gagtacagcc aattactact actttcattt atggtccctc cgattaacat tttgtccttc
     5941 gacaaaacta ctataacaga aataaaacgg atacaacagt ttaatgcttg tgaattcaca
     6001 aaggtttgtt tgtagtttca ttccttttca agcataagaa cccaatatct acaagattct
     6061 tattttaata atattcactt ttatatctaa gattgtgaat aaaagacaaa aaaataaaaa
     6121 agctagcgtt ttttttacac agctagcttt ttttaggtat aacacttgta gaaactgttt
     6181 ttacatttgc gccttttttg ctgactcttt acaatccgga cagagtccat acaattccaa
     6241 ccgatgttca ttaatctcaa aatcagtcag tctgcttgca gccatttcta tatcttcaag
     6301 ccctgggtaa tgaaaatcaa caatcttacc gcagttttca caaattacat gataatgacg
     6361 ctttgaactg aaatcaaatc ggctagaagc atcgccgtac ttcatttctt gaacaaatcc
     6421 aatcgaagta aataagcgaa ggttattgta aacagtcgca acactcatat ttggaaaccg
     6481 atgttcaagt gctttataaa tctcatcagc cgttggatgg gaatgatgtt caatcaaata
     6541 ttctaaaacc gcgtaccgtt gaggtgtgat tcgaatgttc tcttttttta acttttcgat
     6601 aacctcttcg actaacttat tttccatgta aacaccccac gttgttattc tcgtcttatc
     6661 atactatttt tacattttaa aatccactta acatgaaaag aaaattcaat tttattgtat
     6721 tttaatgaaa aaagacgtaa aacagtcgcg ttcactattc tacgtctcta ttcaaataat
     6781 ttttctcatc ataaattttt gtgtgttctt ttcatcttta aaagtttttt acataaacct
     6841 atctggagaa aaaccaagtg ccttatatct cttggtaaag ggctgtagat aaataacgtt
     6901 caccattgtc gggaaccaca gctaatacct tcttgccttt tcctaattct tttgcgactt
     6961 ttagcgcggc agcaattgca gcgccagatg agatgcctac taaaatccct tcttttcgcc
     7021 caacttctct agctgtttcc atcgcttcgt ctccactaat agaaaggact ttatcataca
     7081 cagaagtatc cagtgtttta ggaacaaaac ctgtaccgat tccttggatt ttgtgaggtc
     7141 ccgcttcttt cccttctaaa atagcagatt ctgctggttc taccccgatt aattcgattt
     7201 tcggatacac acgttttaat tctcggccag cgccagtgat cgttcctcct gtaccgatac
     7261 ctgcaacaaa cgcgtccaag ccatttactc caaatgcttg atgtatctca ggaccagtcg
     7321 tcttctcatg cacaagagga tttgcttcat tttcaaattg taatggtaaa aagtagccgt
     7381 tttcttttgc caaacgttct gcttctctga ttgatccaga aataccatcc gcaccaggag
     7441 tcaaaatcaa ctctgcgccg taccctttca tcaataaacg tctttcgata ctcatcgtct
     7501 caggcatcac gatgattact ttatatcctt ttgcaactcc taccatcgac aatccgattc
     7561 ctgtatttcc agaagtaggt tcaatgatag tatcacctgg tttcagtaga ccatcgtgtt
     7621 ctgctttttc tatcatactt aaagcaatac gatcttttac acttcctcct ggattaaaaa
     7681 actcaagctt tacaaaaaca tctgctgaat cttctggtac gattttattt aatttgacga
     7741 ttggcgtttt gccaatcaat tcggtgactg agttatagat agctgtcatc ttcaattcct
     7801 ccttgtcttt gctcttctta cttatcataa tacaatctgt tcatttatgg cgagtgattc
     7861 gtctttgact agcaaaaaaa agacaaagat ataccatata tggtaatttc tttgcctttt
     7921 tatctaaatc aaacgtttag ttcttgacca actaataaag aataactgga catagacatg
     7981 ccatttttcg ccattagatc atcaacagac atgccaaatt tttgtgcaat atcccatagc
     8041 gtatcacctg acacgactgt atatgtctga ccattatttg tatttacatc tgttgttgaa
     8101 gatgatgacg ttgaagtcgt cgtagtagtc gttgttgttg tacttgtggt tgttgtagcc
     8161 gaacctgttg atggcgtatc aaaacgggtt aaattataag tattaatcaa tgagttcaat
     8221 ttggccccat aatttggatc ggtagcataa cgacctgtca agtacgctgt tgcatcataa
     8281 aaacttgttg tattgctttt ccacacgcca gcataatgat aatcgcccgt ggaaaaactg
     8341 gttgaacgaa gaacattcgc atggtcttgg aaggattccc aataagaaga atattttcgg
     8401 aatggctcat tcatcgttac ccattgtccg tttacatatt ctttggttgc catataaacg
     8461 acctgtccgt tatagcttcc tttcacaccg aatagattat aatttggcgc actagctaat
     8521 ccggaagtgc catatgcact ttccaacaac gcttgagcga tcatgactga agcatacaag
     8581 tcattacttg cagcaacttc tgacgcagac cagccgattt gatcgataaa cgcttgagga
     8641 tcagcgactg tttgagatcc ttgttcatct gcatgaacat gttgcggtac cgctgtaacc
     8701 atcatcggcc caactaaaag actcgttcca attaaaggag caatcgcttt tttattgatc
     8761 accatttttc ttttaggtct atggtgccgg gcactgcggg tcttcagctc ttgcattcaa
     8821 attcctcctc aaaataaaaa aacttaataa aaagttctct tcttaaaaca aacttaatta
     8881 attaaattat acttttaatt
//
LOCUS       DACDUU010000096         8747 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.096, whole genome shotgun sequence.
ACCESSION   DACDUU010000096 DACDUU010000000
VERSION     DACDUU010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.096"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            130..951
                     /locus_tag="IWL30_002553"
     CDS             130..951
                     /locus_tag="IWL30_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP9707266.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(1236..2246)
                     /locus_tag="IWL30_002554"
     CDS             complement(1236..2246)
                     /locus_tag="IWL30_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9707267.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(2258..3211)
                     /locus_tag="IWL30_002555"
     CDS             complement(2258..3211)
                     /locus_tag="IWL30_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9707268.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(3201..4271)
                     /locus_tag="IWL30_002556"
     CDS             complement(3201..4271)
                     /locus_tag="IWL30_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9707269.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(4277..5320)
                     /locus_tag="IWL30_002557"
     CDS             complement(4277..5320)
                     /locus_tag="IWL30_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9707270.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(5321..6262)
                     /locus_tag="IWL30_002558"
     CDS             complement(5321..6262)
                     /locus_tag="IWL30_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9707271.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            6346..6693
                     /locus_tag="IWL30_002559"
     CDS             6346..6693
                     /locus_tag="IWL30_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAP9707272.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(6763..8424)
                     /locus_tag="IWL30_002560"
     CDS             complement(6763..8424)
                     /locus_tag="IWL30_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9707273.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
BASE COUNT         2513 a         1771 c         1607 g         2856 t
ORIGIN      
        1 aatctcatac ttccatttat ttttcatatt taaatgataa cctagatttt tcaagtaaat
       61 ccttggtttt ttttcagagt ctgctacaat aaggtaaaaa gtacttttac tgtcaatgga
      121 ggtggtcaga tgaaccgttt tgcttctaaa gaagaagaaa aattatatta tgaacataaa
      181 gcaagtgaag aagaatttct taactggtat caaaaacagg aacgtccaga atatgacaag
      241 ccctctttga ctgtcgatat cgtactgatg tgctataaca aagaagaaga tcagctgaaa
      301 gttttgttga tccaaagaaa agggcatcct tatcgaaatt catgggcatt gcctggtggc
      361 tttgtccaaa aggatgagtc aactggagaa agcgtcttaa gagaaacaaa ggaagaaact
      421 ggtgtggtta tttcaaaaga aaatattgag caactgcata cattcagtac gcctaatcga
      481 gatccgcgag gctgggtcgt cacagtcagc tacttagcct ttattggaga agaaccgcta
      541 atcgccggag atgatgccaa ggaagtacgc tggttcacac tagaacgtca tagtaacaag
      601 atccatttat ctagcggaga agtggctatc tcgcttgatt tagtcactgg agagtcttct
      661 ggcaaagata ctttggcttt tgaccatagt caaatcatct tgaaagcatt caaccgtgta
      721 gtcaataaaa tggaacacga gccgcaagtt ctacaagttt taggtaaaga tttcacgatt
      781 acggaagctc gaaaagtatt tgctaaattc ttagggatcg attataaaac gatcgatcat
      841 tccaatttta agaaagctat gctccaatat ttcgatgaga tcggtgaacg tcctgtagga
      901 atcggccgtc cttcaaaaat ctatcagctc aagccggggc accatgaata aggttgtgag
      961 caaaagattc agacctaatc attcaagaaa aaacgcaaaa acaactcttt gtgagttacg
     1021 ttttttgttt cagaattttg atgtattcgt aaactttctg aataaaaaat taacaatagc
     1081 agaaaaagct cgtacgaact ttttctgcta ttgttttatt ggtttgtcat aattagtgag
     1141 cttttcttca aaagaaaaat tcctagaaac aaaagtcatt aagacttttt attaacaagg
     1201 ctaaccctaa cgctcctatc agtttcattc attttttatc ccttgataac cgaatgtctg
     1261 cccatttttt ctactacttg gctctttctt acggaattgc tttcttctgc tccgtcggta
     1321 ttgatcaaaa gaaatttttt attataccat tgggacttat aacgcaattt tgttattaat
     1381 ttatgccagt cgtgaataga aaaatcagca tgtgcctctt cttttggaaa agattctaca
     1441 aataagtagt catattgttt gaccaatttc ccagataatt gttcaagata atcttctcgc
     1501 tggttctttt gtttcagata aagatccatt acttttctct tttgtttctg ataattgctt
     1561 gcctgatcta acctgatctt tctatggtga gcggagcgga ctttcacatt caatttccgc
     1621 tgtgcatgtt gcagcttttc ttctgtctgg tgaaattttg gtaaagttac ttctagaggt
     1681 cgagatgttt ggatcaatgt cttcggatga taggcgatcc cgatccattt agctaaagat
     1741 tcttttttta ctggcgaaac atcacaaagt agtgcgacat aaaattcttc attatatcga
     1801 gcagaaatcg tagcggagcg aatcgatcct tcaactggcc gatgctgatt tatcttgatt
     1861 agttcttttt gttttggtaa ctttaagtag ccattttcca gatagatcgt acgggtttgg
     1921 ttgttggtcg tataagattg ccacatactt tttttcgttt taagctttgg gaagctggca
     1981 cgtcccttga aataattgcg aaaagcccga tccaaattaa gctgtgcatt tgctaaagca
     2041 agactgtcta cttcttttaa gaatgggtat tcttttttta atgtagcggg tgtgagcgaa
     2101 aaattggcta ctcctgtttg ttgccaactt ttctgccgct ttttcagcaa atggttatag
     2161 gtaaatcgag cacatccaaa agtacgaatc aattgctgtt tttgttcttc tgtcggatac
     2221 atacggaatt taaaagcttt tagcatctat gtccctccta gttagcaacc agaagagatt
     2281 ccttttgtcg ttttagtttt tttgcgtaat atgggacttc ctgctcagtt gcatagacaa
     2341 aatgttctgg tgcgatttca cgaagttgac gtacttgtcc gtcatctggt tcaggttgat
     2401 atttgattct tctgcgtttt ctctcatggt ctggatctgg tagaggaata gcagataaca
     2461 aactttctgt ataaggatga acaccgtgat tatagatttc atcacttgtt cccatctcta
     2521 ataacaagcc gttatgcatt acaccgattc ggtcactaat atgtttaacc atcgacaaat
     2581 catgtgcaat aaatagatag gtcaattgat gttctttttg gagatcttgc aacaaattga
     2641 ccacttgggc ttggatcgac acatctagtg cagaaattgg ttcatcacaa atgataaaac
     2701 gcggttttac tgccaatgct cgtgcaatac caatacgctg gcgttgccca cctgaaaatt
     2761 catgaggata acgagtaccg tggcttggat tcaatccaac agttctcaat agatcatcga
     2821 ctttttttgc acgctcttca gatgatttag ctagtccatt aacatcaatt ccttcggcaa
     2881 tgatatctct gactttcata cgaggattca acgaagcgta tggatcttga aagatcatct
     2941 gaacatcatg ccggaatttc gtcaaacctt ttttatcttt gatcttcgtt atatcttgac
     3001 cgtcgaataa tatctcacca tcagtaggtt cattcaagcg aatgatcgta cggccagtag
     3061 tggattttcc gcttccagat tctccaacta aaccgaatgt ttctccttca taaatgtgaa
     3121 agctgatgtc atttacagca tgtacttcat tcttttttcc tacattgaaa tactgtttca
     3181 atcctttaac ttcaagtagt ttatttggca tcagcaattc ctccttgacc gtaagtatgt
     3241 ttttgtttct ctttaaagat agcccagcga cgttggattt cttctggcgg tgtcactttg
     3301 ggtgcctgcg gagcaagtag ccaagttgct gccttgtgtg tttttgatag ttcaaaaaat
     3361 ggtggttctt gttctgcatc gattttcatc gcgaattcat ttcttggata gaatgcgtct
     3421 ccttttggcg ggttcaataa atccggtgga gaaccaggaa tagcatacaa tcgatcgttc
     3481 gcactatcta atgtcggcat tgaaccaagc agtccccaag tatagggatg ttgcgggttg
     3541 tagaaaattt cttccgatgt tccaacttca acgattcttc ctccatacat gacagcgaca
     3601 cgatcagcga cattcgctac tacaccaagg tcatgagtga taaagataat tgatgtatcg
     3661 atttttgtct gaatttcttt cagcagctcc aaaatctgtg cttgaatagt cacatcaagc
     3721 gctgttgtag gttcatcagc aatcaggatt tccggataac ataccagtgc aatcgcgatc
     3781 acgatccgtt gacgctgacc tcctgagaac tgatgcggat aactgttgat acgtttctca
     3841 gcttcgggta ttccaactaa tttaagcaat tctaatgcag ctttttgtcc ctcttttttt
     3901 gaaactttat tatgctttct aagggactca gcgacttgct tcccaatggg catcgtcgga
     3961 tctaaagagg tcattggatc ttggaaaatc atcgctattt ctttaccacg gattttttgc
     4021 atctctcttt ctgattttcc aacgatatcg ttacctttaa agaggatttg accagcatct
     4081 atattggcgt tgcttgacag caagcgcatg atgcttctgg tcgtcactga ttttccactt
     4141 ccagattccc caacgatcgc caatgtttct cctttattca aatgaaaatt gacaccgcga
     4201 atcgctcgta cctttccagc atacgtatca aaagagattt ctaagtcttt cacttctaaa
     4261 attttcgtca tcgtcttcac tctttcattt taggatcaaa ggcatcacgc aatccatcag
     4321 caagtaagtt aaagccaatc atgataaccg ataaagttac tgccggaatc cataatagat
     4381 aaggcagata caggaatgtc ttgtaccctt cgctcaacat cgttcctaat gaagctgacg
     4441 gaggcgtaag acctaaaccg ataaagctta aaaaggcttc gaagaaaata gcagatggaa
     4501 tactaaacat catttgaata ataatgacac tagagatatt cggcaaaata tgtttgaatg
     4561 caattttcat tcttgattca cctaatgtca atccagctaa aacatattct tgatctttta
     4621 atttcattgt ttgtgcacga acgatacgtg ccattggtat ccagtttgtg atggccatag
     4681 cagccacgat tgaaaaaatt cctggttcaa aaacgaccaa catcaaaatc atgacaacga
     4741 ggtttggaat tcctgataag atttccaaaa aacgttgcat cacgttatcg acacgtccgc
     4801 ctaacaaacc agaaaccaac ccgtaagcta caccaatcgt tacatctagt aatgcagcaa
     4861 taaatgcgat cagcaacgag attctcattc ccataaataa gcggcttaat acatcacgtc
     4921 ccaaaccgtc tgttcctaaa aagaaattta cgtttgaagg gacgtttgct tgagcatatt
     4981 tatcaacaag ttcaccacca accattgctt ttccattcaa tccatcgata ttgattcccg
     5041 gaatacgagg aggcaaattg atataagcca cgttttgggc agttggatca tgaggagaaa
     5101 caaagattgt gatcacagag atcaaaatga tgaccaatag taaaaccatt gaaataacgg
     5161 cagctttgtt tttctttaaa cggcgccatg aatcttggat aaaattcaaa gaaggtgcag
     5221 taatctgttc acgttcttct tcggtatttt tttgtaatgg aagaaattca tccgcaggga
     5281 tatctgcaac tgtttcataa cgctttggta tcatttccat ttatcctcgg ctcccttctg
     5341 aaacacgaat tcttggatca actagaccgt acaatagatc cacgatcaaa ataacaaaaa
     5401 tcagcataaa tgagtaaagg atcgttacag ccatgatcgt aggataatca tttgttgtga
     5461 ttgacttaac gaattgttcc ccgataccag gaatagcaaa gatattttct accaccagtg
     5521 atccggtcat caatgctaca gctaacggtc ctaataaagt catcaatgga atcaaactat
     5581 ttcgaagacc atgtttaaag gcgatttccc aacggctcaa tccttttgct tttgctaatt
     5641 ccacataatc actatgcagt acttccacca tttccgtacg gataaaccga gcagagtcag
     5701 caagcggcga catggccaac gcaattgttg gtaagattgt gtacacaaag ccatcccatt
     5761 ttgcgattgg caaaacttgt aatttgattg caaagatata ttgtaataaa acagcaaata
     5821 caaagtttgg aatggaacga cctaatatcg ctacgagtgt agaagatgta tctgcccatg
     5881 tattttgctt catggcagag atcgttccta aaatcgtccc taggactgtt ccaaagataa
     5941 ttgcctgtaa ccctaactgc aaggatggtc cgattcttcc agctaacaaa tgtgcgactg
     6001 gctggttttt aaattggaag gaaattccaa aatctccttg aagcaaatta gaaagataaa
     6061 tcccatattg tacgataact ggtttatcca aaccgacttg tttatttagc atctcgattg
     6121 tttcaggact caaacgttcc tgattcgtat atggcgtgcc cggcagcaat tgcatcaaga
     6181 aaaaggtgat tgttgcaatt aaccaaagcg tgatgatcat gaaaaagaca cgttttaata
     6241 catatttgat ataactattc actaatgttt ccccctagct tttgacttct gacagatgtt
     6301 tcgctacatt attagataat gaaccaaaaa taaaggagca atcaaatgaa aaaaaaatct
     6361 atcccctacg cagtagcttt tctactgatt ctggttattt taataaaaaa tctcatcaac
     6421 cattccttta cactcattca attatctaat gatctattct tatggtcatt gccgtttctg
     6481 attatcggcg gttttttatg ggtcttttct tcgggtttct tcgatcattt tcagcgatcg
     6541 gtccatcttg cacgaacaag gaaccgaaag aaaaaaccag aattttcctc gctctcgtct
     6601 gccagctacg gtatgtacag cttttggctg atcattgccg gaatactaat tgctctttct
     6661 gctattttca tgcttttttc tttgttagga taagtgaata tgagaacgag gttgcgacat
     6721 ttgccgcagc ctcgtttttt ctcatgctca cggtattaac gattattctt caacgtatgt
     6781 ccatttgtaa tcccatgaag cgcctgccgc atggtgaacg atacctttga ttttttcatt
     6841 cactaggtga ccttctgctt tttggtacac cggaacgaca cccatatcat cagagatgat
     6901 cttgattgct ttttggaaat catcccaacg ggcttgttca tcgccagcgt ctgttgatga
     6961 agctgcttta actgcttcgt catattctgc attgctccag cgaccacggt tgtatgaatt
     7021 acctgtgaca aacaaatcag taaagctgct tgggtctgca tagtcagcag cccatccgcc
     7081 tagtactgcg tcaaaatcac ccgaagttga gcggtcaaga cgtacagaga atggaacagg
     7141 tgttggtttg actgttacgc catccaagtt ttcttggatt gagttttgaa catattcgat
     7201 tacttttttc gatgaatcat catcagaagc catcaaatca aattctaaag aatcaacgcc
     7261 caattctttt ttcgcttttt cccaatattc ttttgcttta tctttgtcat aagaaactaa
     7321 ttttccagct tcttttgcaa agtcttcgtt cgttgttgga ctttttacca tatctgatgg
     7381 gatcaatcct gtagagacaa ctgatccgtc acctagtact tgttttacta aagcttcacg
     7441 atcgatagaa taagaaatag cttttcttag gttcacattg ttgaatggtg aattcttttc
     7501 acgttgattg aattcgatat agcttgtccg cgcttcttta acagaagtat aagctggatc
     7561 gtttgcgttt tgctgtgcta actcaccaga caagatcacg tcatctgctt ggccatcttt
     7621 gaataagttc aatgcagtag aagattcttt tactacactg actttgattt cagaaagttt
     7681 tactgtatct ttgtcccagt aatgatcatt tttcttataa gtccattctg tatctgttcc
     7741 tggtccatca aaatcttcta atgtgaatgg tccattataa acagctttat cacttgcaga
     7801 tgcataggca tccccatttt cttccactac ggattgtttt tgtgggaaaa atgatgggaa
     7861 agcaagcaga tattggaaat atggtgtttg tttcgtcaat ttgatttcta attcatagtc
     7921 accaactgct ttgatcccta gttctgattt atctttcttt ccagctgtaa tgtcttctgc
     7981 attttcaact ggcgcataca ggtaagcata ttcagaagct gtagctgggt ctactgttcg
     8041 ttgccagcca taaacgtaat ccgctgctgt aacaggatca ccatttgacc attttgcgtc
     8101 ttctcgtaat ttgattttgt aagttaatcc gtcttcactg acttctgcca actcgctagc
     8161 tcctgccggc tgtggtttgc tgtcttcatc aagacggtaa attccttcat aaacgttatt
     8221 caatgctgta aaactgattg tatctgttgc aactgataag tcagctgtag gcatttcttg
     8281 aggcacgact aagttaaata cgcctcctcc atctgctgaa ccgctttttg ctgaactact
     8341 gcttgtggct gaactgccgc cataacatcc agcaagtaca aggccgcata ccgcaaccaa
     8401 accaaatgta aatttctttt tcattttacg taccccttta ttggaatttt cagaataatt
     8461 aatattttct gaagttgttt aagagtttaa tgaataatta ttaaaaaatc aacacttttt
     8521 tttgtctttt tcataaaata atgatttttt cgtgaggttt caaggaattt ttcagaaaaa
     8581 atagctttgt acaaggtttt ccatggtctt ccaaaagcca ttaaataatt ttcgttatat
     8641 tgtctattat tgccatacaa agataaaaaa gaataaacaa aaagaaactg tgacaaaagt
     8701 cttgtcacag tcccttatct gaataaacgg tgttcaaaag ccagttt
//
LOCUS       DACDUU010000097         8494 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.097, whole genome shotgun sequence.
ACCESSION   DACDUU010000097 DACDUU010000000
VERSION     DACDUU010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.097"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..173)
                     /locus_tag="IWL30_002561"
     CDS             complement(<1..173)
                     /locus_tag="IWL30_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9707274.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="IWL30_002562"
     CDS             complement(593..1714)
                     /locus_tag="IWL30_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAP9707275.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVMDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYKPG"
     gene            2042..3451
                     /locus_tag="IWL30_002563"
     CDS             2042..3451
                     /locus_tag="IWL30_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAP9707276.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /locus_tag="IWL30_002564"
     CDS             3448..4176
                     /locus_tag="IWL30_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9707277.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="IWL30_002565"
     CDS             complement(4304..5215)
                     /locus_tag="IWL30_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9707278.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="IWL30_002566"
     CDS             complement(5232..6239)
                     /locus_tag="IWL30_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP9707279.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="IWL30_002567"
     CDS             complement(6236..8365)
                     /locus_tag="IWL30_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9707280.1"
                     /translation="MKKKLFRIVGIVFISIVLLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8477)
                     /locus_tag="IWL30_002568"
     CDS             complement(8370..8477)
                     /locus_tag="IWL30_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707281.1"
                     /translation="MQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2600 a         1726 c         1485 g         2683 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtttgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccatca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca ataggacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DACDUU010000098         8337 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.098, whole genome shotgun sequence.
ACCESSION   DACDUU010000098 DACDUU010000000
VERSION     DACDUU010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.098"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            156..386
                     /locus_tag="IWL30_002569"
     CDS             156..386
                     /locus_tag="IWL30_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAP9707282.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            483..1784
                     /gene="murA"
                     /locus_tag="IWL30_002570"
     CDS             483..1784
                     /gene="murA"
                     /locus_tag="IWL30_002570"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP9707283.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1797..1973
                     /locus_tag="IWL30_002571"
     CDS             1797..1973
                     /locus_tag="IWL30_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP9707284.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2108..2404
                     /gene="yidD"
                     /locus_tag="IWL30_002572"
     CDS             2108..2404
                     /gene="yidD"
                     /locus_tag="IWL30_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAP9707285.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2588..3055
                     /locus_tag="IWL30_002573"
     CDS             2588..3055
                     /locus_tag="IWL30_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9707286.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3140..4024
                     /locus_tag="IWL30_002574"
     CDS             3140..4024
                     /locus_tag="IWL30_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9707287.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4141..4374
                     /locus_tag="IWL30_002575"
     CDS             4141..4374
                     /locus_tag="IWL30_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAP9707288.1"
                     /translation="MSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4462..4542
                     /locus_tag="IWL30_002576"
     tRNA            4462..4542
                     /locus_tag="IWL30_002576"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4496..4498,aa:Tyr,seq:gta)
     gene            4549..4620
                     /locus_tag="IWL30_002577"
     tRNA            4549..4620
                     /locus_tag="IWL30_002577"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4581..4583,aa:Gln,seq:ttg)
     gene            5135..5842
                     /locus_tag="IWL30_002578"
     CDS             5135..5842
                     /locus_tag="IWL30_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9707289.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5902..6546)
                     /locus_tag="IWL30_002579"
     CDS             complement(5902..6546)
                     /locus_tag="IWL30_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAP9707290.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6673..6960
                     /locus_tag="IWL30_002580"
     CDS             6673..6960
                     /locus_tag="IWL30_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707291.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7068..7388
                     /locus_tag="IWL30_002581"
     CDS             7068..7388
                     /locus_tag="IWL30_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9707292.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7469..>8337
                     /locus_tag="IWL30_002582"
     CDS             7469..>8337
                     /locus_tag="IWL30_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP9707293.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKK"
BASE COUNT         2741 a         1353 c         1797 g         2446 t
ORIGIN      
        1 ctcagtccct ttttttgcat atttttagca tttatttcct aaaaaatgta aggggattcc
       61 tatattttta agtatacttg tttttccttt tatacatttt atggtattat cattttatta
      121 gttattataa aacaatcgtt agaaaggacg tcattatgca agtatacgga attgatgcgg
      181 tgcttcgaat cgtgagtcat ttggcgttta tctacttagc cttctggtct ttacgttcgt
      241 tacgcattga aacttttttc agaccaatgc ataatacgca aattagaatg gcaatcgtac
      301 ttttttcaat ttttttaggg tacactgcaa gtaatttttt cttagaagtt atcgctttat
      361 gccgaaatct tttcgttagc ctgtaaattt taaaaaaagg tactatttta aaagcaagga
      421 tgttataata gaaaagattt tgagtaagtg tatgctcatt ggaatttttt ggagggaaca
      481 acatggaaga gatcatcgta agaggtggca atcaattaaa tggaacagta agaattgaag
      541 gagctaaaaa tgctgttttg cctattttag ctgctagttt gttagcagaa gaaggtatta
      601 cgacattaga caacgttccg attctttctg atgtcttcac tatgaatcaa gtgattcgtc
      661 acttaaacgt agatgtagat tttgatgaac aaaagaatca agtaacaatc gatgcttcaa
      721 gacaattaga aatcgaagcg ccttatgagt atgttagtca aatgcgtgct tcaatcgttg
      781 tgatgggtcc gctacttgcc agaaatggac atgcaaaagt agctatgcct ggtggatgtg
      841 cgattggtaa acgaccaatc gatttgcatt taaaaggatt tcaagcttta ggagcaaaaa
      901 ttattcaaaa aaacggctat atcgaagcaa ttgcggatga attgatcggt aacacgattt
      961 atttagattt ccctagtgta ggtgctactc aaaatatcat gatggctgcc gtaaaagcaa
     1021 aaggaacaac catcattgaa aacgtagctc gcgaaccaga aatcgttgat ttagcaaaca
     1081 ttttgaataa aatgggcgct caagtctatg gagctggtac tgaaacaatg cgtattgaag
     1141 gcgtggatca tttacatgca gtcaaccact caatcgtaca agatcgtatc gaagcaggta
     1201 cctttatggt tgctgctgca atgacacaag gcaatgtgct gattgcagat gctatctctg
     1261 aacataaccg cccattgatt tctaaattga tcgaaatggg tgcagaaatc attgaagaag
     1321 aaggcggagt ccgtgtcatc ggaccaaaac atatcttacc tacagatgta aaaacgatgc
     1381 ctcatcctgg tttccctaca gatatgcaag ctcaaatgac agctatccaa ttagttgcag
     1441 aaggtacaag tgtagtgact gaaacagtgt ttgaaaatcg tttccaacat ttagaagaga
     1501 tgcgccgtat gaacgctcat gtcaaaatcg acggaaatgt agcaatcatg gatggaaacc
     1561 atgaattgca aggtgcagaa gtatatgcaa cagacttgcg ggcagcagca gcattagttt
     1621 tagctggctt gaaagcaaat ggaatcacac gtgtgcgtaa cttgaattat ctagaccgtg
     1681 gatattataa tttccatatc aaactacagc aattaggtgc agacgttgaa cgtgtagaca
     1741 tggatcaaac ttctgctgaa aaaacagcac aaacgattgc ttaaggaagt ggctgaatga
     1801 gtaaccaacg atatatctta ttaactttga taaagatttt agtggtgatc ttattgctta
     1861 tccttctttt tgtggccgga acgatgatcg gatatggtgt catcggtggc ggcaatccat
     1921 ttaaagtatt ccagccaagt ttgtggattc atattcgaga cttttttcat tgataaataa
     1981 gaatcacagt ggttctagtc gatatgaagt gagccatgaa gcagaacatg acaaaagaaa
     2041 acagaaggga acgttgacgt tagtcgcgat cccttttttt agtaaaagaa aagaaggaat
     2101 ctaattgatg aagaagatat tgatttattt agttagagga tatcagcgct ttatttcgcc
     2161 tgcatttcca cctagctgcc gttattaccc aacctgttcc aattatatga taaaagcaat
     2221 tcaaacccat ggtgcactaa aagggacggt aatggggact gcacggatct tacgctgtca
     2281 tccttttgtc aaaggcggta tcgattatgt accattgaca ttccaattaa cgcgaaataa
     2341 gaaagataaa aaagaaccag attataaaaa ggttgtgaaa aatacgagta gcactgagcg
     2401 ataaagaaac aatttggaaa atagcttctt atattttgca aaattgtgag ttattgccga
     2461 agtgttggat tttgaacacc gtcaaaaaaa ggttgtgaaa aatacgggta gcactgagcg
     2521 ataaagaaaa agaggaaaat tattgtcaaa gtaacaactc cgcaactact tatatggggg
     2581 acaaaaaatg atcgaagaac taaacaagaa agatttacca tggtccttgt tattagatgc
     2641 agatcctgat aaagaaaaag ttcaagtata tgtttccaga gggagtggct ggatatggaa
     2701 agaaaaagaa aaaacaatag gtgtactgat ctatgtggca agggaaaaag aatacgaaat
     2761 cgtcaatgta gcagtagccc caacgtatca aggaaagggg attggcggaa aattacttga
     2821 gacagctttt cagaagcttt cacaactcgc ttccagtcaa actcagatca tcatacgaac
     2881 cggcagtact agcagtgcgg cgttacatct ctatcaaaaa atgggctatg tggagattgg
     2941 tcgagtcaaa gactatttta tccatcatta ctctgagccc atttttgaaa atggagatca
     3001 attaagggat caagtaattt tagcaattca cttacctaaa aatagcttta actaataaac
     3061 tttcaattcg agaaaatgct ttcaaatgct aaatagatac attacaatag ctttagcaaa
     3121 caaaaaggag aatattttta tgggaatatt agtttttgat tttggtggat cggctgtcaa
     3181 atatggttgt tgggatggca aagagattaa aggtaaggga caatttgcaa cgcctgaatc
     3241 ttgggaagaa atgaaagcgc aattatttca agtttataaa aaaatactcg tttcttttga
     3301 agtcgaagga attggaatca gctctcctgg agtagtgaat aatgagaaac aggtgattga
     3361 gggaatcagt gcgattcctt atattcatgg atttaatatt ttccgagatt tggaaactct
     3421 gtttcaactc ccagtaacga ttgaaaatga cgccaactgt gccggaatgg ctgaatttta
     3481 tgaaggtgct gcccgtgatt atcaagatgt tgcttttgtt gtggcaggaa ctgggattgg
     3541 cggggcgttg ttcacgaaag gacaaatcaa taaaggggca catctttatg gtggagaatt
     3601 tgggttgatg ttcttaaatg gcgataagac attcagtaaa cttggtacag cggtacagat
     3661 ggcttggcgt tattgtgaac gcaaaggtct cgataaaaat tcttataccg gaaaagatgt
     3721 ctttgaatta gcagaaactg gagatgcaat agcaaaagaa gaagttgaga gtttttatac
     3781 gtatcttaca aagggattat tcagtattca attttcgttt gatccagaag tgatcgtatt
     3841 aggcggcggt gtttctgcaa aagaagggct cattgatgag atcaaaagcc gaatgaagaa
     3901 gctgacagaa caattcgatc tgcatgattt cgaaccgcag attttattat gtgaatacag
     3961 aaacgatgct aatcttgtag gtgcagcagc aaactatctc gctgtacgtc aaggggagaa
     4021 ttaagagctt tagagaaggt gcagcttgca taaaagaagt gaatgaaatg aataggagat
     4081 ttttacctaa ggaatgtttt atttgctgca gtatgataaa atatctgaga ggtgaaaatg
     4141 atgtcaaaaa aaaataaaga tgttgaagtg cgtatagaag aagtgaaaaa agatatcaaa
     4201 ggtaagaatt atgaagtcac tcagttattt atagggaaaa aaatgattgg tgagatctta
     4261 gcctatgggc caaaagaata tgaaattttc tttggtgaag aagattttgg aaaagaaaaa
     4321 agtcttgaaa atgcgataga aacagtgatt cgacactgga atctgcacga ataaaaaaat
     4381 atcaaaaaaa ctttcaataa agggttgcaa aaatctggaa aattaggtat tataactaag
     4441 tcaggttgat acaaaccttt cggaggagta gcgaagtggc taaacgcgac ggactgtaaa
     4501 tccgttcctt agggttcagt ggttcgaatc cactctcctc catttcattg gggtatagcc
     4561 aagcggtaag gcaagggact ttgactccct catgcgttgg ttcgaatcca gctaccccag
     4621 tataccacaa tcgtaagctc tcgttagttc gagagctctt ttttttgctt tgctaccttg
     4681 ttccttgaag attctgctga tttaagatag agttaagtta actacaagaa ttggagggat
     4741 ccacatgaaa tggaaagaaa aagtagacga agcagctgaa aagttatatg atttggtcaa
     4801 atcagaaaaa tacaatattg aagtgaatat tccgaaaaaa ggcggaaaag cggtccaggt
     4861 aaaatcaaaa cgcccaacga atcatacaaa aaaatggatg gctaaaaacc gctaataata
     4921 acaaaagatc cataagtcgg cattttcatc gattttaggg tcttttgtta ttttctaggt
     4981 tgaacatatc taaaaaattg tttctgacaa taggagtagg aagaacgatg tgtgtctaaa
     5041 tcctctgttg atggatagaa acctttatct ttttcagttg tgaatcaggt atataccagt
     5101 tataatagaa gaaacgaaag gacgggtgta cgttatgtcg aatcaattac ctgtatatat
     5161 acagattcat gaccaattaa aaaatgaaat cgaacaaggt atatggaaag tcggagaccg
     5221 tcttccttca gaaagagaat tagcagtgaa attcaatgtc agccgtatga cgttgagaca
     5281 agcaattcaa aacttggcag atgaaggaat cttagaaaga aagatcggtt cgggtacata
     5341 tgttgcgcga gaaaaagtac aagaaaaaat gtctggtgca acaagtttta cagaaattat
     5401 ggaatcgcaa aatcgagtac cgtctagtcg gacaatctct tatttcttga cttcgccaag
     5461 ctcaagtgaa atggaaaaac taaacttgag taaagatgac acgatcttgc gaatggaacg
     5521 tattcgtttc gcagatgatg tccctatttg ttttgaagtt gccagtgtgc cacaaaagct
     5581 gatcgatggc tacagcaaag cagagattac tgaatcgttg tatcgcacgc tagaagaaaa
     5641 aggcgggaaa aagatcggtc cagcgaatca aacgattact gctgtgttgg cttctgagaa
     5701 aattgctgac tatttagagg taaaacgagg agatgcagtt cttcgtttgc gccagatttc
     5761 ttattttgaa gacggtacgc cgtttgagta tgtgcgtacg caatacgtag gaaatcgttt
     5821 tgagttttat ttggaaaaat aaaaaacagg tgatccctga ttccttactt agaaataaaa
     5881 gagaatcaga atcacctgtt tttatatatg atcagttacc agttgctcgt gataagaaac
     5941 aggaccctct tcaaattgga cttgtccgtt cagcagctca atgatggatt ctttaaagtg
     6001 gtcagcactt ttttcatcta cagcacagat gacgctaact tgatcagtat attggatttc
     6061 ttttataaaa aaatgttgga gttcaagata attctgtaat tttcctagtt gcggatacgt
     6121 gatcatcaca gagatctcct gttgcaaggt accttctacg attccaatgt cagacaatgc
     6181 ttgagagaca gcatgagtgt atgctcgtat taatccgcct gtgccgagtt tcgttcctcc
     6241 aaaatatcga gtgactactg cagctacatt gatcagttga ttttttttga ggacttccaa
     6301 catcggtacg ccagccgttc cacttggttc tccatcatcg ctgcttcgct ggatttcatt
     6361 tttttctcca atgatgaagg cgctgcagtt atggttcgct ttccaatgtt ctttttttat
     6421 ttcttgaatg aaactttttg cttggtcctc tgattctatt cgtctaagag aacagataaa
     6481 gcgtgatttt ttgatctcaa tctcactttg tccattttct tttattgtac gatagcgctc
     6541 gatcatcttg tcacctcttt ttttcatttt ctttattcag tataaataaa gttctaggtt
     6601 gtttcaacac tgtaacggtt gcgcaatcgc tgtagtatgc gataataaag acaaaggtag
     6661 gtggatctgg ggatgcagga acgaatgaaa aagtacgatg caattacgca ttatttgaag
     6721 aacaatggcg gctctcaagt aacgttgacc tttacccaat ttgacgaact gctgtttccc
     6781 agtaacggac ttcccaaaac agcaagggaa agtacagatt ggtgggcaaa tgattataaa
     6841 caccctgaaa aaggtgccta tggctggata aatgcaggct atgaagtagt tgtcatcaat
     6901 ttggacaaag aatatgtcgt gttcaataaa ttagtgaaat ctagttggct atttgattaa
     6961 ataaaccgcg tttagaaaac gttgacaaat ttctttcttt ttatataata gagtaacaga
     7021 cgcgaagaaa acgtttgcgt agtttgtttt aaaaaggaga gaaaagtatg gctaaaaaaa
     7081 caatcatgct tgtatgttcg gctggtatga gcacaagttt actagtaaca aaaatgcaaa
     7141 aagctgctga agcaaaagga atcgactcgg atattttcgc tgtgtccgct tctgacgcag
     7201 ataacaatct tgcaaataaa gatgtagatg tactgttatt aggacctcaa gttcgcttta
     7261 tgaaagctga ttttgaaaaa cgtctggaac caaaaggcat tcctttagac gtcatcaata
     7321 tggctgatta tggtatgatg aatggcgaaa aagtgttaga tcaagcaatc acgctcatag
     7381 gtgaataaat agaacagtat aactatcgaa caagcgatcc aaaaaataga tcgcttgttt
     7441 tttcgtatat ttttttaggg cgaataatat gaatcatttt tggggtagaa ggcttctgac
     7501 gagagatatt aaagcaatag actgtgagga ggtaaattta ccaaattatg agaagatcgc
     7561 agcagtggaa cgtttgggca actggattct ttgtcatcgc tgtggaatga ggacgtccgt
     7621 cattgatgga cagcttgcag attacggtta tttttgtaca cattgtctat atttagggcg
     7681 atgcgacact caacaagatt tatatttgtt tgatcagcct gaagcaaaac ctcgagaagt
     7741 cgtctttttt tggacaggac agcttactga aaaacaaacg gaaatcgcaa agagaatact
     7801 ttgccattca gaaaaaagac atcatcttat ttgggctgta acaggtgcag ggaaaacaga
     7861 gatgctttat cctattttag tggaaacttt aaaagctgga ggaagagtag cgatttgtac
     7921 accgcgagtc gatgtttgta atgaattgtt tttacgctat cgttatgttt ttccagaaga
     7981 aaaaatcacg ttgctccatg ggaatacagc aacggcttat acctttactt cttttgttat
     8041 ctgtacgatt catcagctat accgttttta tcggtccttt gatttaatag tgatcgatga
     8101 gatcgatgcg tttccttata acggggatgc aggattagct catgctgtgc aaacagcgat
     8161 taaaccagac ggacgattca tctacttaag tgccacacct gataaaaagt tactaaagga
     8221 aatcaagcag acgtttgaca tccacaaact tgctttgcgc tttcatcggc gtttacttcc
     8281 tgaacccgtc ttggttttca tgaataattg gcgacaaaaa tgcaagaaca agaagaa
//
LOCUS       DACDUU010000099         8262 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.099, whole genome shotgun sequence.
ACCESSION   DACDUU010000099 DACDUU010000000
VERSION     DACDUU010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.099"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(158..1534)
                     /locus_tag="IWL30_002583"
     CDS             complement(158..1534)
                     /locus_tag="IWL30_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9707294.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            complement(1654..2301)
                     /gene="eda"
                     /locus_tag="IWL30_002584"
     CDS             complement(1654..2301)
                     /gene="eda"
                     /locus_tag="IWL30_002584"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP9707295.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(2385..3812)
                     /gene="uxaC"
                     /locus_tag="IWL30_002585"
     CDS             complement(2385..3812)
                     /gene="uxaC"
                     /locus_tag="IWL30_002585"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP9707296.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(3886..4965)
                     /gene="uxuA"
                     /locus_tag="IWL30_002586"
     CDS             complement(3886..4965)
                     /gene="uxuA"
                     /locus_tag="IWL30_002586"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP9707297.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(4968..6599)
                     /locus_tag="IWL30_002587"
     CDS             complement(4968..6599)
                     /locus_tag="IWL30_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP9707298.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            6903..7574
                     /locus_tag="IWL30_002588"
     CDS             6903..7574
                     /locus_tag="IWL30_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9707299.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
BASE COUNT         2717 a         1612 c         1103 g         2830 t
ORIGIN      
        1 taatattatt ccaatcctgt aggaattttt ctctatgatg aacatcaaaa atctctgcag
       61 caattggtcc gaaaaagaga ttgtgtttat aagtcatcgt atccataaca cagccatctg
      121 aatcaataca aaatatccaa ttattcatat ttctctccta tttatgtttt tgttttaaaa
      181 gctcgcaatt ttctaagact ttatctttag ataatggata aaggaaaaat aaaattaatc
      241 ctacaatcag aaaacaaatt gatggaatta atgttgaagc gttatataac ttttctaaaa
      301 catctcccgt ttgtacattt gctaaagagt catagcctat aatagaaaga gtccaacctc
      361 cgacgcctcc agctagagct tgacctattt ttctagcaaa agaatatacc gcatatacgg
      421 tcccatcttc tctttgatca gactttactt cttgatcatc aataacatca gtaatatttg
      481 cccaaataac agtattaaac acacccaaac ccatatagcc aaccgaacta agaattaaga
      541 atacatacat attactagct tttatcaaaa ataagatcaa atatactatt gctgaaaaga
      601 acattcctac tgatgccagt tctttttttc catatttttt acttaaaaac aaagttaaag
      661 gcgaaacaac tattaaaacc aaaataggat tgataaaatt cattaaaact attccctgtg
      721 catcattata gtaatcagga aatacataat tattcatact cataattaat aattgagcaa
      781 ggagtaagaa aatagccgca cctattatag aaagtaatgc cctattttta actaaccctt
      841 ttaaagattt taacaatgaa actttgtatt gagattggtc aattcttact cgttcagtag
      901 ttagtttata acatattaaa taacaaataa gtgcacatat cgaaaatatt cccgcaacaa
      961 ttgtaaaagt tgatccacca cgaactattt gagcgcctga atcatctctt gtataaataa
     1021 ataatggtgt taatacacca atgaccaaac ttgctaaagt agctccaaca tttcggaatg
     1081 tcgatagcat tgttctttct tctgtaatag aagtaatagc tgatgccatt gatccataag
     1141 gaatatttat agatgtgtaa aaaatactcc cccataatat atacgtaaca tacatgtata
     1201 ctaccttaac gctcatagaa gctgaaatca tagaagtttg atacattaaa aaacttgcta
     1261 aagcgactgg accactcatt cttaaaatcc acggtttaaa cttaccctgc ttagttgctt
     1321 tcgatctatc aacaatacgc cccatagtta catcagtaac tgcatcgata aatcttgcta
     1381 ctaaaaataa tgttccaacg gcagctccgc ttatccccaa tacttttgta taaaagatca
     1441 ttaaaaaaga actagcaaaa atgaaagtaa aatcgtttcc aaaatctcca aacatatatc
     1501 ctattttgtc tcttatacca aattttcttt ccatacaatt ctctcccttc tgttgttaag
     1561 cgttttcatt ctgatgaaag tacgtactat actctgaagc gtaaagagtc agtatttatt
     1621 ttttacattt cagagtatta tttgtttttt ctcctaataa ttctttatct cataaaactt
     1681 atctatatat ttactagcta ctcgacttac ttcttcaaaa tcaccttttt caacagaagc
     1741 caataaattc ccacctacac caactgcaac aactcctact tcaaaccaat cttctaagtt
     1801 ctctatactt actccgccag taggcattat atttacgtgt ggtaatggag cttgataggc
     1861 ttttacaatg gatggaccaa aagcagaacc tgggaatagt ttaatgatat ctacgccagc
     1921 agtaagtgcc tcctttatct cagtaattgt catacaacct ggtaaataag gaatttgata
     1981 taaattacaa atctcagctg tttgtcgatc aaaagatgga ctaacgatat attctgcccc
     2041 agccataatt gcgatacgag cagtaaacgc atctaaaacc gtaccggctc cgattattac
     2101 atctttttga tcagcatatt ttttagcaat ttctttgatt atttcttcag cattaggaac
     2161 tgtaaaagtt agttcaatcc ctttaattcc acccgcaata attgaattta ttgctttaat
     2221 ggcctcatcc ttatttttcc cacgtacaac cgcaatgact ccagccgctt ctaacttact
     2281 taaaatttct acttttttca tatctatctc cttcctttat ggatacatac tttgtctagc
     2341 tgtactaact tacaaagaat aaatttaagc ttttatttca agtattattt tctacttccg
     2401 atttcaaaaa aattaaaata attatacgca ttacgataac aaatattctc cactatctga
     2461 ctaagataca tttcatcatc tggaattcgg cctgctttaa cccactcgct tagtaattga
     2521 cataatactc gacgaaaata ctcatggcga ggatagctta gaaaacttcg agaatcagtc
     2581 aacatacctg taaaattcga caacaaactt tgttgtgcca tcgtagtcaa ctgttgacgc
     2641 attccttcag ctgtatcatt gaaccaccaa gcacatccca attggatttt ttgaatcata
     2701 cccccttgga agctttgcat tgctgttgct agtggaagcc aatcagttgg atttaatgaa
     2761 tataaaattg tttttggtaa gcacccactc ttttcagcgg tatcgaacaa tgctacaaca
     2821 ttttgtgtca aactagcttg atcccccatc gaatcaccac cgacatctac tccaatttgt
     2881 tcctttaata tacttgaatt atttcttaaa gcatttaaat gcatttgcat cgtccaacca
     2941 tatcttgtat actcagacat caaatgtaat tgaatacctg taacaaattg tgcaatttct
     3001 gataaatgga aatccgtttt tcctgctaaa gcttttatta acagattgtc gagtattttt
     3061 tcatctgcta tttcataaaa ataagtattc aaaccgtgat ctgctaatcg acagccaatt
     3121 tgatgaaaat gatccactcg ttggctcaat gcttgcttca acccattaaa gtctttgatt
     3181 ggtatagccg ctgtttgact aagttttaaa atatagttcg aaaagcctga tgatttgata
     3241 ctccagacct tatctggacg gaaagtaggt aataccttga aagaattatc ctgtttagaa
     3301 agctcagtat gataccacaa gtcatctgta ggatcatccg ttgtacagat ggctttcaca
     3361 tccatcatct ccaacaattc tttcgggcga taggaatccg tttgaatttt ttcgttagca
     3421 cgtcgccaaa tttctggtgc attcttctca gaaattgtca aatcaatatc aaagtaacgt
     3481 tgtaattcca aatgagacca ttcaaaaata ggattacctg gcgccttctc cactgttttt
     3541 acaaaagcta aaaatttttc ataatcatca cctggaccac tgatcaactc ttcttctata
     3601 ccattagctc gcatcaatcg ccatttataa tgatctccag ctcctttatc atttaaccat
     3661 acctgagtca aattcttgta ttgcttattt tctagcaaat atgctgggtc taaatgacaa
     3721 tgatagtcga taattggttg gtgttttgca aatttatgaa aaagtctttt agctatttca
     3781 ttctctaata aaaagttctt atctaaaaac atatttaaac actcctttcc ttccgttagt
     3841 ttttagaaga gaaaattttt cctctaaata aactccctta tataattatt tactcgcccc
     3901 cttttcaagc gcttcccaaa taccatttaa ataaactgca cctagagctc ggtcatataa
     3961 tccataacca ggtctaccgg tttctcccca aatcatccga ccatgatctg gacgaatata
     4021 gccattaaat ccattatcgt acattgtttt taatatctta tacatatcta gagaaccata
     4081 ttctgattta tgcggcgctt cgtaaaaatc gagtccattg tcacttaaat atttgatatt
     4141 acgaacatgt gcaaaaggaa tccgatcccg ttgacaaaat tcagatagaa tttcatagac
     4201 atcattatct ggtcgttctg caatacttcc agtacataat gtaataccat tagaaggaga
     4261 atccactaca ttacacaacc aatctaaatc gtcacgattt ttaacaatac gaggtaatcc
     4321 gaaaatggaa tatggtggat catctgggtg aactgccatt ttaatcccaa cttcttcaca
     4381 tgtcgggata attgctttga gaaaatatgc aaagttctct cgtaatttct cttcatcaac
     4441 ttctttatag gcttctaata attgcttaat ttgtgataac cgatgaggtt cccaacccgg
     4501 caattcaaaa ttattagtag aattttctac ttctctaata atttcctccg gatcagtagg
     4561 gatatttttt gaaatatagg ctaacgtaga agacccatca tctaaacgat aatccaaatc
     4621 cgattttacc caatcaaata ctggcataaa attataacag ataacctcaa tcccaaattc
     4681 tgccaaattg cgaatcgtct gtttataatt ctcaatgtat tgatctcgtg ttggtaaacc
     4741 aattttgata tcatcatgaa cattaacact ctcaataact ttcaaggtaa gtccattggc
     4801 ttcgacttca tcttttaaag atttaattcg ttctctcggc caaacctctc cggctggtat
     4861 atccattaat gttccaacaa ttcctttcat tgctgggatc tgacgaattt cttgtagtga
     4921 gattttttct tcatttgccc cataccatct aaaagtcatt tccatagtta ctttcctcca
     4981 acttctgtta tatatttatt gattgtttta cgaactgctt gaggacctac tagcatttcg
     5041 caaaagattt tttcagtttt ctttcctacc ccaacttgat ataaatctaa accaaagatt
     5101 tctttatttt ctaaaatttc ctgtaccgca caatgaatct ttgaaggatc ttgttcacct
     5161 aatttcaata ctgataattt atcctgcaac tctaccaata acggatcagg acttggcaca
     5221 aaattttccc cttgatcatc gactgccagt aaataacgta accaagcagc aatagcgata
     5281 ggaataaatg ttagattttc tgcatttccg tctttttcga catacttttt aattgtttca
     5341 ccatagcgaa tcgctacttt ttgagaagta tctgttgcaa ttcgttgtgg tgtatctgga
     5401 atatagggat taggcaaacg tttattaatt acttcatcaa taaattgttt tggatcaata
     5461 atgcctggat tttcaactac tggtaaacct tctaaataac ctattccttt gattaaacca
     5521 accaaatcat catcctgcat ctctgctgaa attgattgat atcctagcag acaacctgtt
     5581 actgccaaag cagtatgaag tggattcaag cttgtcgtaa ccttcatgat gtctgcttta
     5641 tcaaccgtct ctctatctgt cataatcaca ccagcctttt ccaatttagg ccggccattt
     5701 ggaaaactat cttcaataac taaataatga actacctcag tattagtgaa agcagcaata
     5761 ttggtatgtt tttttgtgtg aatgatatct aaatcctcga gccccatcat caatagatct
     5821 tcctttacat tctctgaagg atttggcgta attcgatcaa tcatactcca aggaaaagtc
     5881 actttttctt tatcctttaa ataagcaata aatttttcag aaacatatcc ttctttttgc
     5941 caagcttctg caatcgtaaa aatactatct tgaaaacgtt gaccattctg tgagaaatta
     6001 tcagtagaaa ccatcgcaat cggcaactct ccagcattaa atctgctata taacaaggat
     6061 gttacaatac tcatcgtatg ttgaggattg ctaggtccag atttgatatc agctttgact
     6121 accgaaaaga attgcccttt agaatctttt aaactgtaac ctttttcagt aatagtaaac
     6181 gttgtaaact gtaatttagg atcttcaaaa tatttaacca ctctttgata gtccgctata
     6241 ttcttcggat tgcaatataa actatcagcc gttactgcca aaagtctttt tttgagttca
     6301 ccatctccac tcataacaat ttccaaaatg tcattgttat agtcatgata agctttttcc
     6361 actacttctt catcaaatgt ttcacacaca actactcctg tttctaattc attttgatct
     6421 gctaagcatt gagcaatttc tgcatgaaat cctcgataaa gatttcctcc accaaaatga
     6481 agccatactg gatgttgctg gccttcttgc ttaacttctt tgacatcaaa acacggcagt
     6541 agaatctgca tttcattgaa ttcttcgata gaattttgcc aatttttaat cgaaaccata
     6601 attaacctcc tcaaactgat atgttagttt tgatacattt tatattttaa aacccattgt
     6661 aaccgttgtc aatataaaaa gggaataaac ttgctttttt ctaccatatc agaatgattg
     6721 tattttttat tcagaaaaat aaaatactcc ttctttttgg actgatttta tcgaaactta
     6781 ttttttcttt ttttcatgta ttcttttatt tttataacta gtgtattact tcaaaatttg
     6841 atattatatg gcatgaacag aattaaaata tcagttaaaa taaaagttaa ggagcattgt
     6901 gtatgacaat taaaactagt ctccaaaatc aagcttacca atctattcgc cgacaaatta
     6961 tctatgctga cttagaacct ggaaaaaaag tatcagaaaa aaaattagaa gaaacacttt
     7021 ctattggtcg tacaccaata cgtgaagcac tgattcaact ccgacaacaa ggtcttgttg
     7081 atacaattcc tcaaagcgga aattatatct cattaattga tttagattcg gccaaaaatg
     7141 ctcgatttgt cagagaacat ttggaacgac aaattatgtt agagtgttgt gccaaaatga
     7201 ataaccaaaa aaaacggatt ttagaaacta taatttcaga acaagaaaaa gctgttagtc
     7261 aaaaaaatgc tcgtagtttt tttcataatg ataatttatt ccataaagct tgttttgaaa
     7321 ttgcagagcg agaagaagta tggcaatggt tagaaaataa caatacacac ttagaacgat
     7381 ttcgatggct acgagtaaaa acggaaggat taaagtggga aaaaattatg gaacaacatt
     7441 atcaactatt tgaggcatta acaaaacaag atccagaaga agctaacttt ctaacatcgt
     7501 tgcatcttca tttaatgctt aatgagcaaa atattgtttt agctactcat ccaaaatatt
     7561 tcaaaaatat ataactttta actcttttta aattaaatgg ttgttctgat cagaaaaata
     7621 tcgcttgttt tcttattatg taatatattt cataaactat tctgcataac aactattgtt
     7681 tgtacttttt acattggata actaattatg actgctgata acttttgata ttaaactagt
     7741 atctccaata caaataaaaa atagatatct tgttcatcaa aattagttcg tggatactta
     7801 ccttcattta catcattcag cggtttccca ttttctttta ttctattatt tcattttcta
     7861 atagacttgg cttcatttcg caatactttt tttcaatagg catcaaattg gtgttgttta
     7921 gatatttagt tgtagttttg tgacatgata agtatgctcc tttttctctt ttccactaat
     7981 acaacgatca aagggtgctt ttttcaaaaa actagaaaaa tcaaaataag attcgtattt
     8041 tgattctata tatcctgttt caactacatc ttatatttca caatagaata aatagccttt
     8101 tgcatagctg cttaacttat aagaaattgc aatttacttc ttcaaagtga aaacgaatat
     8161 gagtaaaata aaattattct tcttacagat aattaaaaat agcgcgtaac aaaaatcggc
     8221 attcaatcac ataattagga ttgatgccga ttacttatat tc
//
LOCUS       DACDUU010000100         8011 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.100, whole genome shotgun sequence.
ACCESSION   DACDUU010000100 DACDUU010000000
VERSION     DACDUU010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.100"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..366)
                     /locus_tag="IWL30_002589"
     CDS             complement(<1..366)
                     /locus_tag="IWL30_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9707300.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSY"
     gene            complement(363..605)
                     /locus_tag="IWL30_002590"
     CDS             complement(363..605)
                     /locus_tag="IWL30_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HAP9707301.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            complement(1151..1681)
                     /locus_tag="IWL30_002591"
     CDS             complement(1151..1681)
                     /locus_tag="IWL30_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9707302.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            complement(1863..2519)
                     /locus_tag="IWL30_002592"
     CDS             complement(1863..2519)
                     /locus_tag="IWL30_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAP9707303.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            complement(2646..3119)
                     /gene="dfrF"
                     /locus_tag="IWL30_002593"
     CDS             complement(2646..3119)
                     /gene="dfrF"
                     /locus_tag="IWL30_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAP9707304.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            complement(3538..3756)
                     /locus_tag="IWL30_002594"
                     /pseudo
     CDS             complement(3538..3756)
                     /locus_tag="IWL30_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3787..5001)
                     /locus_tag="IWL30_002595"
     CDS             complement(3787..5001)
                     /locus_tag="IWL30_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP9707305.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(5342..5584)
                     /locus_tag="IWL30_002596"
     CDS             complement(5342..5584)
                     /locus_tag="IWL30_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9707306.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(5616..6173)
                     /locus_tag="IWL30_002597"
     CDS             complement(5616..6173)
                     /locus_tag="IWL30_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9707307.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(6186..6374)
                     /locus_tag="IWL30_002598"
     CDS             complement(6186..6374)
                     /locus_tag="IWL30_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP9707308.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(6387..6953)
                     /gene="amaP"
                     /locus_tag="IWL30_002599"
     CDS             complement(6387..6953)
                     /gene="amaP"
                     /locus_tag="IWL30_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP9707309.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         2430 a         1629 c         1145 g         2807 t
ORIGIN      
        1 atacgatgta ctaggaataa tttcttcata aatcttgcca taagtttcat atattttatc
       61 catagcacca atatgctcca caaccaaata tttataagac ggtatttctt tatacagaaa
      121 gccctctggg gtaatggttc tttggggaat agaacagaaa taataaagtt ttccatttct
      181 tctttccata aaagcatatt ttacccaatt tcctgattgt gaaagatacg cttttttcaa
      241 actactgtta aactttctcc aaaactgtgt actaatctga atattctttt tttgataatt
      301 agtcagttct acttcctgcc caataactgc aaatgcgttt aattccttta tagaaaaatt
      361 tatcatttta ccttgtgagc agtgataacg gtataactgt acgcatttac cgttataata
      421 caattatcaa tcgttatata ccagtttttt ccttttcttg tgatttccgc attagaaagg
      481 ctaatttttt ctttgcacca ttcaattaca ttttctacat tcaaagataa gttcttttta
      541 attctaataa cgccaagttc agttgtgtga agtcttccta agttttccag taattcattt
      601 tccatatttt gatacctcat ttttcagcaa atttatttgt atgttttata ccccaattat
      661 aacattctca tatcagcact acaataaaaa taattaagca ccaataatct tattgaacat
      721 ctccaaccgt aaatctttca caccatcagt gataaacact gagctgacag caattacgac
      781 aactaccata attgcctgca cttaaatttc tgcaaaagaa aacgacaatc aatttcttat
      841 tttttgaaat caactgtctt tctcatattt actattcaat tttcatagtg atataggtac
      901 aatttatatc actactgtaa aaatggggtt ttgccttata aatacagcgt tttccgcttg
      961 ttagtgtata gtgtttcttg ttttttgtgc cccccttgtt agataacggg ggctttgaac
     1021 ggctcgcaag ctcgccgaac cgccgaagcg aaccgccccc ttgcaagcat ggcggaaaaa
     1081 tcttaaggat tttcccgcca acgtggtcgg atcgctccgt cggtttaaca gcttttctgt
     1141 ttctctctag ttatctacgc atataaatca aatcggttat tccgttataa gtctgtgttt
     1201 tacaaagcct taactttatt tccctgtcta tgttgcccaa aagacgaacc ccatttccta
     1261 acagagtagg aataacagaa atgtaatatt tatcaatcat atttttgctt accaactgct
     1321 gaacaagatt tgctccgcca caaatccaaa tatcctttcc tgcttcttgt tttagccgtt
     1381 ccaataataa aacaggattt tcacttgtaa aatgaatttg ttgggaagaa ttgcactctt
     1441 tatgtgttaa tacataagtt gtaaatttat tatataccca ttcttgcgga gaaagttcag
     1501 taacaacctg atgagaagta ttccacccca ttaagacagt atctatatct ttggtaaatt
     1561 ctgtataagt gtcgatgttt tcattgtcat ttccatgacc atttagccaa gctactccac
     1621 cattaccgtc cgcaatatat ccgtcaaggc tcatggcaat aaataaaact acttttctca
     1681 ttttttctct tttataaata caaacaggaa tttatttctc ttttttactc ataagggaac
     1741 aactgattgc tacaaataaa cccattaaaa tccatggaaa tgctgctctt ataaaatcac
     1801 taaccatact ttattcctcc ttaaattcca atttccttag ttctacaaac tgaaattttt
     1861 gcctatgaat gtatcttgtt ctttatacgc aaacaggctt ctgtgtgcat aatccaatgt
     1921 cttgaaaacg gcatttgaat tagtccacaa atatctttat tacaccaata atcaatcagc
     1981 caaattgctt tttcattgtc gtttactaca ttcaacgatt ctaagtatcc ttttctttct
     2041 ttcggtattt ttcttttcaa ttcatcataa gataaccgtt caagcatttt ctcagtgctt
     2101 tcccatactt cgaaaatata taaatataat tcttccagat taagctgttt tgaaaagtct
     2161 gcaatctgct gtttcatgag ttcatttcct gttgtaatga taggtgaatt gatacgctct
     2221 tgataattgc ctgcaaaaaa tacttgttca tcttcattta tcactgtatg cacaactata
     2281 tcttcaatgc ggaaaatatg ccaaatagaa taagcaatcg tcttactatg ataaccatca
     2341 gcgtttatga aggggattga gttaaaatct tctctgcata attcttcttt gaaagatact
     2401 aaggtttgta ttaactgact tcgtaaagta agtaaagtat caatacccct cttataagta
     2461 tctttctttt ttatttgtgc ctgcattgtt ttattaagtt cagaccattc cttattcata
     2521 ttcaatcctc tctcaaattc tgattttatc gccccataca aactggaatt tgtcttacaa
     2581 caatttatat atgcataaaa agcaatatat ttatgaaaac accctaaatc caaaatttac
     2641 tcaattcatt ttttccttac ataaaatgtt ctcgtatatt tcacttcctc accaagtgtt
     2701 tcccctatca aaacttcaaa atcattgata tcaaattctg gaaagaatgt atctccatct
     2761 tcaatattta aatctacttc tgtgatatac attttatcta ctatttgcaa agcttcctta
     2821 aatagtccat atccaccaga tatgtataca tctcgtcctt tagccaataa taatgcatct
     2881 tctaatgatt taactgaaac taaattatct ccttgatact ctgttgtggt ggaaacaaca
     2941 atattcattc tattaggcaa cggatgaccg atttcttcat aagactttcg ccccataata
     3001 accacattac ccgttgttaa ctctctaaat tgcttttgtt ctccctttat tttccatggt
     3061 atattaccat tcttgcctat aacattattc tttgacctcg caacaatcaa acctatcatc
     3121 tatttcatcc tccataatat gatgtcgtac ctgtgattcc atcaagtgca ccacatctta
     3181 acaaactcaa aattatctcg aactaaccag ctcgacaaat tccttttttg tcttcatctg
     3241 cattatatca tattgttata agcattgcca tttcttttct gcttttgaaa agttacttat
     3301 cagcctatgt tttgtaaagg gtgatattca gcatttctca cgcaacaagc ccttgacaaa
     3361 ccatagacta ataagtttta aggtaatcaa gcagaaatga aatagtctgc tgtttcacgg
     3421 caacaaaaaa gagataccaa cacacttatg tagtatctct tagtctagta gcaatattct
     3481 tttgtttgca agcaggtaaa catgggctgt ttcttaatat ctttttgttt gggaaacacc
     3541 tttttcccat cctttcaccg ttgccatctc tgctgcgcac aatgccacag ggataaacca
     3601 tagcaaaccg cctaataaca agaaaaatac taagctgaag cctgaggtcg cgaatgtggg
     3661 atattcatat aatgaaacga ccattgagat ggtcatagcc aaaaagccaa agaacgttaa
     3721 tttttttgca ggaattttcg ttttgctttg ttgcatgaat cttcctcttt tctatttgaa
     3781 tttgtttcaa ttgagtttgt tgccaaagaa gaaaaagtcc aatccttctt cttgttcttc
     3841 ttttgtagta gtgatcgttg taaagtgcat cgccgatttt aaaatcactg ttgtcagcac
     3901 ataggcatac acggcagtaa tgatgacacc aatcacttga atccctaaga agtaccagct
     3961 gcttggattc gttagtaaat ggctgttaga tgcaaagatc cctatcagaa tcgtacctgt
     4021 gaatccgccc atgccatgaa caccccacac atcaagtgca tcatcccatt gtttcttttt
     4081 acgaaaatca acagcagcat gacagacgat acccgcaatg atcccaatca gcatggcact
     4141 ttttgcttct acataaccgg cacaaggcgt aatggctgcc aatccagcaa cggatcctgt
     4201 tagtacgtcg acaaaatcaa agccatttcc ttttactttt gcaatgatca gccaagtaat
     4261 catcgcaaat gcaagagcga tgaatgttga accaaatgca ttgactgctt gttcatctgc
     4321 tcgaagtgca ccaccggaat taaaaccgaa ccagccaaac caaagcagtc ctgtaccgat
     4381 agcagctgcc ataagactgc tatgctgact tctttctcct aaaggaatct ttctttgacc
     4441 aagtgtgagg atacatgcta aagagccgaa tccagctgtc gtgtggatga ctgctccacc
     4501 agcgaaatct ctaaatccca tctgatccaa aaatccgcca ccccatatcc agtggcaaac
     4561 agggaaataa atcagcagat tccaaaaaat aacaagtata atatagcctt ttaaattcaa
     4621 tctccctgca aaagctcctg tcatcaaagg aacggtaatc acgataaaca tcagttggta
     4681 aagaaagaac attaaaaatg gaatcgttgc accatgaaac atgttgggga agcttgtaac
     4741 atgtctcatc aaaaagaaat ctgccgggtt tccaataaca ccgccgatat cccgtccaaa
     4801 ggccaatccg aatccaccaa atacccacat aatcgttgtt atcccaacgg agagaaaaga
     4861 ttgagacatg atcgttagta tattattctt ccgtgctaat ccgccgtaaa aaaaagctag
     4921 accaggagtc atcaggcaaa ccattgctgt acataaaatc atgaatgctg tatctcctgt
     4981 attgataggt aatgtattca tttttttcgc ctcctttcta cgattacgag tataaaagaa
     5041 ggtgtagttg tttttttctt ctgttttttt gaattaatac ttgcatttat aaaagacaga
     5101 caattaataa aagcttgatg tgttgtgctc tttttcgttt ttcacacaaa taaagacaaa
     5161 aaataaacgg tcttcttttt atagaaaacc gtttattttc gtcttttttt atttttaaag
     5221 atattttttc ataaggaata aagaatactt catataaaat atataaaaaa gaaccgcagc
     5281 gagtgtcgct acggttctca aatttgttct tcatttcaac ataagttttt cttcaatttg
     5341 cttataattt acgtgcaaag aatgaaacga ctaatactaa aatgattgca ccgatcaatg
     5401 atggaatgat agccattcct gcaattgttg gtccccaagt tcctagtaaa gcttgtccaa
     5461 ttgctgaacc tactaaacca gcaatgatgt ttgcgatgat ccccattgat gatcctttat
     5521 tcgtgattgc tcctgcaatt gctccaatga taccgccgac gattaatgcc caaataaatc
     5581 ccattttaaa ttcctcctaa ctgacttctt acttcttatt gtacacgtgg ttcgttattt
     5641 tctttgactt tttgtgcacc tttgcctaca gcagattttg ctttatctgt ttggcgtgaa
     5701 gcaaattgtc ctgtggattt tgctgcttct gttactttgt cttgtactgt ttcttgatct
     5761 ttttggtatt cttcttgtgt tttgatgtct gcaacattca cattcacttc gataacatct
     5821 aaatgtgtca tattgtttac ttcagtactg atcaattctt tgatttggtt gtaaatatct
     5881 tcgatgtctt taccgtattc tacgatcaca tctagatcca cagctacttg tttcttacct
     5941 acttctgtat tgatacctgc tgttacgtta tctgtgttga ctaatttttc agccatattt
     6001 gagaaaaatc cgccatctaa tgtcaataga ccatctacat ctttcaatgc ataaccgatg
     6061 attttttgga tgactttgtc ttcataagtc aattcaccat tcatcatgcg gttttctcct
     6121 ttttcatttg ccaaaactga tccatttgtt gtattcatgt ttttgttttc catctttgtt
     6181 tcctcctatg atttaaaaaa ttgatcaact attcctgact gtttcaaata aaaaccgatt
     6241 cctgcgccaa ttgctgtcat gattatgatc aacaatgtct tgaaaaatcc aatcgttaac
     6301 cataaaacag ctagcagtag tccaagaccc ccaaagataa tagggagctt gtatgctgaa
     6361 aatagttctt tcattatttg gccctcctat tccactcgag agtgcttgta gtctcttgta
     6421 tcttcttgtt cttcataacc gctgtatgtg actgccactt tgaccgtttc ttttgttccg
     6481 agtactttag ttacttcttc ttcgatatct tgcatcaata cacctgtttt ccctactaat
     6541 gaagaagttc gtttcaagtc tccgttgaca tggatatgga ttcgattttt cgttgaacgt
     6601 atccgtactt taggtgaatg aatgaactct tcttcgtgta aatgggaacg taccaaccct
     6661 tcgatcgctt ttttgtcgat tgttagtttg ccatcttccc gtttcataac gaaagttcct
     6721 cttgactttg gatagaaaag aacgactagc aagatgataa tagcaagaac ggcaaatact
     6781 acagctgccc agaaaatata ttgttgcatg tagtaattcg ttaaagtaaa gaagcgaaat
     6841 cgttccaatt gttcaggcat aatatacggc gcattgatca acgcaatcgc tagaaatact
     6901 gaaagtagca gtaaactcat gataacaccg attattttta accccttatt cattttccac
     6961 ctcctataaa cttattttta ccctgtaaat aactctaccg acaatcacag tatagatagc
     7021 gaacagttag catagcatca gtaaataaag cgctaccaaa cattgctgga tcataacggc
     7081 gttttattgc cagtctgata ctgaaataac aattgcttac ttattctcta tttacttttg
     7141 attgctggct atacttaaag gatattattc aagattaaaa attgcaaaga ttatgctcag
     7201 ttattattac aagattctat ttatttatta aattttttcg gagaattaaa aaatacaata
     7261 aataacttta gttactttaa tgcgttctta ctttattttt atattatcat ttttcttatt
     7321 cacttctttg gtttatacta ttcttataaa taaaaacgag gtatgtccta tgatgaaaag
     7381 attgtactat tcattgatca taacgattgg ctatttgatt gtatcaaatc taggaaatat
     7441 ggtatttggt atcagtaaag aattcagttg gactactaca ttatgggaat ctctattctt
     7501 tttcatcttc gtatttttgt tacaaaatta tcgaaaaaaa taaacatgtg ccatctatct
     7561 gttggcacat gttttaggat ttatggtact ttttattcgg caatccattt tatttttcta
     7621 cttcttctta atacctctaa cacttttgat ttataaacta atccaatgca tctacttgcg
     7681 cttattaagc tatttcagct agatagtacc accccatgcg tcaatgcttg attttccttt
     7741 tgtataacat tgtataagca ataaaggaca aataagtggt gattacacca aaccattgaa
     7801 taagaaaaat tctgatacgt aaataaacat gaacttgaaa aaattaatga atcggttagc
     7861 tactacgtag ttgtcttttt tctcatacag ttctgttaac ttacacttct cttcgctaga
     7921 taataaaaaa tcctccaact gtattgtcca atataatccc aggcatctaa actagcatta
     7981 tagaagagac acacatagaa tctaaaataa c
//
LOCUS       DACDUU010000101         7766 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.101, whole genome shotgun sequence.
ACCESSION   DACDUU010000101 DACDUU010000000
VERSION     DACDUU010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.101"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..113)
                     /locus_tag="IWL30_002600"
     CDS             complement(<1..113)
                     /locus_tag="IWL30_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9707310.1"
                     /translation="MKKKIFVGAIIALFLLPINAFAYSINNEFNLGANEGSS"
     gene            complement(110..499)
                     /locus_tag="IWL30_002601"
     CDS             complement(110..499)
                     /locus_tag="IWL30_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP9707311.1"
                     /translation="MTGVIGGTIVGLLGGMDNILHVLIFLVGVDFLTGLAKAWKLKEI
                     SSEIGFEGLLKKVLIFVVIAVAVDVQKIVGNSIPLREIVIMFYVANEGISFLENISVF
                     LPLPDKLKEVFQQIRNDTENKDRGGTK"
     gene            complement(599..739)
                     /locus_tag="IWL30_002602"
     CDS             complement(599..739)
                     /locus_tag="IWL30_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP9707312.1"
                     /translation="MNSFPGFENIKQFYDWGCYTDQDLLDYVNMNCLTKDQYKQITGN
                     EI"
     gene            complement(732..1079)
                     /locus_tag="IWL30_002603"
     CDS             complement(732..1079)
                     /locus_tag="IWL30_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707313.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKSGTAVDDIEDVDQLKQLNALLTKQLAISVQEQEKMQQMLAQLTM
                     EVASIKNGGKSNE"
     gene            complement(1093..1587)
                     /locus_tag="IWL30_002604"
     CDS             complement(1093..1587)
                     /locus_tag="IWL30_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707314.1"
                     /translation="MADIVQLKEDGVAKYLKTHVKAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            complement(1587..2036)
                     /locus_tag="IWL30_002605"
     CDS             complement(1587..2036)
                     /locus_tag="IWL30_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707315.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            complement(2040..2660)
                     /locus_tag="IWL30_002606"
     CDS             complement(2040..2660)
                     /locus_tag="IWL30_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707316.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            complement(2660..3568)
                     /locus_tag="IWL30_002607"
     CDS             complement(2660..3568)
                     /locus_tag="IWL30_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HAP9707317.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            complement(3581..6343)
                     /locus_tag="IWL30_002608"
     CDS             complement(3581..6343)
                     /locus_tag="IWL30_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP9707318.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLAEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRLIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     SLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFTSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSAGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWRIYDILCNNGFTK
                     QSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLF
                     NAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQAAYAFERNYE
                     RPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYS
                     GYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIIN
                     WARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISS
                     IVIPPK"
     gene            complement(6340..7080)
                     /locus_tag="IWL30_002609"
     CDS             complement(6340..7080)
                     /locus_tag="IWL30_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP9707319.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYRNVTRKIECYYKAPSIDVVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            complement(7070..>7766)
                     /locus_tag="IWL30_002610"
     CDS             complement(7070..>7766)
                     /locus_tag="IWL30_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP9707320.1"
                     /translation="KDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVNSA
                     ENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTIKG
                     IAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTPSV
                     QPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQMK
                     RDAGAVGGTLYAT"
BASE COUNT         2258 a         1644 c         1068 g         2796 t
ORIGIN      
        1 gaactacctt cgtttgcgcc caaattgaat tcattgttga tcgagtaagc aaaggcgttt
       61 attggcaata aaaaaagagc tataatagct cctacaaaga ttttcttttt catttggttc
      121 ctcctcggtc tttattttca gtatcatttc gtatttgttg gaatacctct tttaacttat
      181 ctggcaaagg aaggaatacc gaaatgtttt ctaaaaaact aatcccttca tttgcaacat
      241 agaacataat aactatttct cttagaggta tagaattccc aacgattttc tgtacgtcaa
      301 ccgcaacggc aatcacaacg aagattaaga ctttcttcaa caatccttcg aaacctatct
      361 cactacttat ttctttcaat ttccatgcct tagcaagtcc agttaagaag tccacaccca
      421 caagaaaaat taaaacatgt agtatattgt ccatccctcc caataaacca acaattgtcc
      481 cgcctatcac ccctgtcatt actgagagtg tatttaaata tttttccaat atctatttct
      541 cctttatgca aagaagcgca ctcgtttgag tacgcttttt atttaacttt gtgcttaatt
      601 aaatttcatt tccagtaatc tgcttatatt ggtcttttgt taaacagttc atgtttacat
      661 agtcaagtaa atcttggtcc gtataacatc cccaatcata aaattgtttt atattttcaa
      721 agcccggaaa actattcatt acttttccct ccattcttaa tacttgcgac ttccatcgtc
      781 aattgagcta acatttgttg cattttttct tgttcctgta ccgatatcgc caattgtttt
      841 gtcagtagag catttaattg cttcaactga tcgacatctt caatatcgtc aaccgctgtg
      901 ccactcttct cttcttcagt agctaactca acccactgtt ttttatcaaa atcaaatctt
      961 ggtctccagt taggaactgg tggtttaatc tctgtacagt tttcagggat attttcttga
     1021 ttgttcaaaa tgatttgctc gaatccgtaa ggcttgattg atttgtaaac tactttcatt
     1081 agatttcctc ccttaaattg aataagtgat aacaaatgaa taagctgatc cataacttga
     1141 atttcttctc catttaatgg ctccgtctgc gccaatagat agttgagcac tgttcaaagt
     1201 agaacggtct attgacccaa ccagttgttc aaaactaatt ggtgtccgat agccttctgg
     1261 aacagttaaa ataaccgaat cattcccgcc actacttttt ccgtttaaag ccacaaaata
     1321 tatagaaact gtttttcctt cacgataaag ctttgctgtc cctgtattac cgttagtgac
     1381 aaccagattt accacttcat atctgttatc atcagtcgtc aaaacacgtt tattattgat
     1441 tgttacagaa cctgaaaaat tcttatgtcc tgcaatggct tggtctccat ctgtctgcac
     1501 caatgcttct ttaccatcaa tagcttttac atgcgttttt aagtatttag caactccgtc
     1561 ttcttttaat tgaacaatat cagccattaa accgtcccca ctttctcaaa tgtaatattg
     1621 gctaatccat caagtttcac tttatcggtt gctgacatta aacctgctgt cgtagttgta
     1681 gcattacctg gatttttctg tgctccagct gcaattccat ccaacttagt tttatctgtg
     1741 gacgacatca acccatttgc tgtagttgta gctacagctg tagttgtggc atttattcca
     1801 ggatcacctt tatctccctt tggtaaaaca aaattaaatc tagctgcaga tgatgttcct
     1861 acattcgtaa cagaagcggt tgaaccacta gaaacggttc ctatggtaat tgttgctgct
     1921 tggccaggat cgcctttatc tcccttcacc gttgttggtt tgccttctat agcattccaa
     1981 tgagtttgtg gataaacctg tacattgctt tgttttattt ttacgatatc tgtcattctc
     2041 tatacctccc caattttttc aaaagtaaaa tttggaattc tttcgtttgt gtaattttct
     2101 gcttgattta cagcttcttg gaatttttga tctacatatg actgattgac accaccagtc
     2161 ccactaccac ctgtagaact aattgttcca tcttcatcaa tagtgatgtt ttctccagca
     2221 gtataatttt tcagtgaatc gagtttttct tttaactcca gcgaaaaatt gacatcagat
     2281 tgtctgactg cagagacttt ccctgtctca tcaatcgtca ataattcacc aacttttatg
     2341 ccacccaaac ggtcgatagt ggcaactggc aacacgtagt caccttctcc actgtttaaa
     2401 attttttgat acatttctgc cgtgataatg ccgtcatctt cttcacttgc atagggtaat
     2461 tctgtgagaa cattctctaa tcccaaatct tctttagtaa tgacgacggc tccaattttt
     2521 ccattaacag ataaaactgt tgcttctcct gctattattt cttctaaccc aagaactgcg
     2581 gaagcatgtg tgattggaaa gaattggcgt tgtacacctg ttttttcatc cgtttccatc
     2641 attcgtttag ttttagccat tagatcaccc cgactttctc aagagtgaac acattttgct
     2701 ttggatcatc aacggttgcg atgatcaagg ctccttcttc aatcgggaat tctacagttc
     2761 caactttttc gacttcgtga ttatctgaaa agagatcgtc ttgaagaata tcagtcactt
     2821 caatttcacc gtattcaatc gtgaaaagtg ctgctttcat tttttgatac aaataattta
     2881 gatcggatag gaaacgttca gaaatcgatt catggcgtac accttgaaga tctacacgtg
     2941 catccattaa ttcggctaac attactccac ctggatcgat agacttcaaa atatccttga
     3001 ttgattcaaa ccatgacaaa taatctgatt cttgtccttc tctccaatct tggaaactat
     3061 tctcctgttc ttctctccag cgatcaaatt cttctttcct ttcattcatc cagtccgtaa
     3121 aatcgccttt attttcatta ataaaatctg tcatatcagc aattaaatct tcaattgatt
     3181 gccaataaga acccatttct ccttctgttt ttgaaacagc attgatgaca aaataggaga
     3241 agtcttgagt tgttccaatc aagtcttctc ctttaaatat aatgaaattg gctgtttgtc
     3301 gatgcaaaca ctgcatggaa tatttatcaa aaatatattt gattttccct tttttagcat
     3361 ccacaattct tgtttctaat tggactggat attttccgcc aacaactgat tcaaaatata
     3421 ccttacattc tgataaatca tatggaagac cattttcgac aattgtagct tccataactt
     3481 cagtgttttt attgccttgt cgaacttgaa tcatccccac gtaattatag ggttctgttg
     3541 tacttaatat gacattccac tttgccatta aatcacctcc ctattttggt ggtatgacaa
     3601 tggaagaaat tgcacttgca ctataatact ggcgatctaa ctttccacaa atcataccta
     3661 actctgtgtt ttgttcataa gtttgcatac gaccattagc taagcctcgg ataacgccag
     3721 tatgtccata agttccgtct gcaaaccatg aacccacttg tccacctctt gcccaattaa
     3781 taattgcacc aactactaat tgatcatatc tagggttttg aatcactttc caaccaacag
     3841 cagaccaatc ataggcaata ccaatatcag atgctgcaga agtatttcct atcacatgag
     3901 ttaaaccata ttttgttcca gcacccatgc cacaaccacc cagatatcct gaatattctg
     3961 cagacaagcc ataacactga ccattgccaa ttcgttgtcc aatcaaagac tctaaatgtt
     4021 ttagtcctgc ttctccagta gctcctcctg gttttaaatc tttaaatttg ttataccagt
     4081 taactgcata atcttgacgt tcaggatgtg tcgctgccgg acgttcatag tttcgttcaa
     4141 aagcatatgc tgcttgtctt ggatcagtac aggccttaaa tccatcaacc gtcgtaggtt
     4201 gtactacgcc catccattgt ccatttgtaa atgtccaaat aagcaatcga acttgtgcat
     4261 ctaagctcgt tattggttct ttaatacctg cagcattaaa taagttttga acataaactt
     4321 ttccatccca tgttgctgga ccaacaagag gatatgaaga accatcccac tgaactaatc
     4381 cgtatgctgg tccacctatt tgaacagtat caggatcaaa agttcctcct gtttcttgtt
     4441 gaatgttccc caatattcca catgcagatt gtttcgtaaa tccgttatta cacaaaatat
     4501 cgtaaattct ccaagctctc ttttctgcat ctgtttttaa ttcactagga tacccaccag
     4561 tagattctcc acctcccgat ggaccaccac cttgtccagg aattatttct tttcctttaa
     4621 ctgtaagttt tccttgtaca tctaagtctc caaaataaat tgctttacca tttcctaata
     4681 aaactaatcc ttttcctact ttaggagaaa tcaaaatata tttgccgtct ccatttgtac
     4741 gaataactaa agaattatct tcaatagggg ttggagtaga agccccagga aaaggattac
     4801 cagcagaatc agttgttcca atcgttccaa ttgaaccctt agaattccaa aattccattc
     4861 cttttttagt taactccatt attttctttt tgttgttcca aatttggagt agtccattaa
     4921 ctaatttcaa tacatctcca gtcttgttaa aagaattttg aaagatatct gctttgatta
     4981 atccagtttg tataaaattg gcattaaata tactatctaa tgtccaagct gaagtaaaag
     5041 gcccccgcca tcctttctta gaaaaagcaa tcccattttt attcattctt agtacttctc
     5101 ttgccttttc taaatctgga ttatccataa taaagatgtt ggaaggtttt tcttttggcc
     5161 ataaacttac atatcctcct gcactgcctt gacctgtaat cattgaagaa acataatcat
     5221 taaaatcact catataatta ttcgttgcat aatcttttaa tttatcctga atagttacag
     5281 cttgttgctg ataaaaagca acttgaatgt ctcctgcttc taatttcaaa gttttttcag
     5341 ataaagaatc gtattgtagt ccactaacct ttgattcaag ataaatatta tattttttat
     5401 gatatatttt aaatgtatca aacagaccat aatttctaat cttcgcaaat tctttcgctt
     5461 cttcactatc agtcaactta tcaaattcaa ccgtaataga aactttaggc ttatcacagc
     5521 caggattgat cgttttaaag taatttttag ctattttatt taagctctta atgtctttta
     5581 ctccttgttc ttctgtaaac tgaacatgct ctgtatacac atcaggatag ttattaatat
     5641 aggcactatc tactggcgaa ccataaattc gattagtcgt acctacctca ctttgaggat
     5701 cagcatatgg aataagtctt gtttttatac ctgtccaatc taatttgacc tttaaaccag
     5761 acatatcttt tccataccga atcgttccaa cattattgcg acctcttcgc tttaacaaag
     5821 aaagcttaaa tggttcacgt ttaatttctc caccccaata ttgaagcaat gatccttgtt
     5881 caccagcaat acaatttaaa acatttctcg cttcaaagat tgtgctagaa acggccgtaa
     5941 tatcagaata aagtcgtatg tcagattttt tatccatttt ggtttcaata attgatatcg
     6001 cttcttgacc agttttagag tctacttccg caagagtgac tacccgtctt ccaagtcgat
     6061 ttgtacgact ctgagcataa atagttactg tatttaaaaa agtatctata tctttatcat
     6121 caataaaaaa gatatgatac tcttcttgat cgtttggctt tgcttttatt tgatagtcat
     6181 tttcaaaata ttcatcaaat ctagttccta aaggataatc caactctaat tcatattttc
     6241 cattagctac ttcatatatt tcacatcttg tacaatcctt taaaattcct aagccattcg
     6301 ttgagaaatc tgtttcagta ggactatata ttcttggttt catacttttc tccaccacct
     6361 aggcattatt tcaaatgaat gaatattttt cgtccattta atttcatttt ttccaggata
     6421 taaaaatgga aaatctaaaa ataaagtgac ttgatcctga tgttctaaat taccatctaa
     6481 ttttcggtaa gcctcttcta atttagaatc tataaataat tctctgtcca acgatttcaa
     6541 atcatatttg tccttattaa tataaaaaga agcatctcca gaaccactca atttaataag
     6601 cggttttgaa gaatacttct ctgggttata aatttcaaaa gtttctgttt gtcgaatagt
     6661 aaatctacca ttgtaatttt ccttaaatgg tcgaatgctc actgtaaatt caaaagggac
     6721 aatatttcct gtttttcttg ttcctttaaa ttcaggtcct tctattacta cagcttgata
     6781 aatatattgt tcatcatagt aaagaataaa atcactataa gacgacatat ctaaccattc
     6841 agtgattcga tcttcccact cctgcaccac atcaattgaa ggtgctttat agtaacactc
     6901 aatctttcta gtcacatttc tgtaatagcc tttatcaata atgatagaat catttccttc
     6961 tcgctctctc aattcaatta ctcgactagc tgaaacagaa gcaggcctgt tttgaatata
     7021 cacattaaat tcagaagaat aatgttgatt aataaaaaat tgtcctctct taagttgcat
     7081 ataatgttcc tcctactgca ccagcatctc gtttcatttg tcttgtcaat tctgttttaa
     7141 tttttgtggt aatcttccga accattgaat caggtaaatc tccataaaca tttaaatgta
     7201 aatgaatttc ttttgtttca ctagcagata tattttgctt tgtttgtgta attggtaggt
     7261 gacttattcc atttactaca ggttgaacag acggtgtttt tactaaattt gtcatggttt
     7321 gatctaactt actttgctct ttatcaatac caacgataat accttgaaca atatttttac
     7381 caaccatatc acgcatccat cttgatggag aatgaatatc taaagctcct ttaatccaat
     7441 ttttgatatt acctgcaata cctttgatag tatctttcaa agcattccac ttttctttta
     7501 caccatttat aagtccatca atgatgttct tgccaatttc aaataaattg acttccctta
     7561 acgaattaaa gatttctttt acccgattaa tagcatttga aacaccattt tttagatttg
     7621 tccatgcatt ttcagcagaa ttaacaattc cagtaacaat attccagaaa gagtccttaa
     7681 tgttattcca tgtattaatc atggtatttt ttatagaaat ccacgtattg tatgccgtat
     7741 ctttaatatt tttccaagta tctttg
//
LOCUS       DACDUU010000102         7727 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.102, whole genome shotgun sequence.
ACCESSION   DACDUU010000102 DACDUU010000000
VERSION     DACDUU010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.102"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(27..2198)
                     /gene="copB"
                     /locus_tag="IWL30_002611"
     CDS             complement(27..2198)
                     /gene="copB"
                     /locus_tag="IWL30_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAP9707321.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(2218..4404)
                     /locus_tag="IWL30_002612"
     CDS             complement(2218..4404)
                     /locus_tag="IWL30_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAP9707322.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDALFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(4404..4613)
                     /gene="copZ"
                     /locus_tag="IWL30_002613"
     CDS             complement(4404..4613)
                     /gene="copZ"
                     /locus_tag="IWL30_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAP9707323.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4626..5066)
                     /locus_tag="IWL30_002614"
     CDS             complement(4626..5066)
                     /locus_tag="IWL30_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAP9707324.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5140..5679)
                     /locus_tag="IWL30_002615"
     CDS             complement(5140..5679)
                     /locus_tag="IWL30_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HAP9707325.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITGFPLLPLDLLWHTTDYSIQA
                     KKWFIQQQQSFMETNHSWIIEGNYTSTLGKRIKEADKIIWLQVPRCRAIYRVILRSLK
                     RKINKKTRPDMPETFSERFDKEYLEFLSFIWHFEETHEPEIQQLIQENHAQNKLIILK
                     TRGEKQEFLTKLKIESKNN"
     gene            complement(6173..7639)
                     /locus_tag="IWL30_002616"
     CDS             complement(6173..7639)
                     /locus_tag="IWL30_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9707326.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         2087 a         1700 c         1355 g         2585 t
ORIGIN      
        1 ttttttgtca caaccttttt tcattattat ttgattttca atgtcaaagc atttagagct
       61 acaactaccg tacttaacga catcaagaca gcaccaactg ctggacttaa tatgatacca
      121 attggcgcaa gaattccggc tgctagcgga attgcaagaa tattgtaacc tgccccccac
      181 cagaggttct ggatcatttt ctttcgcgtt tgcttggcta gatcaagaaa atgcaaaata
      241 tcttttggat cgctgtttgt caatacgaca tccgcagaat cgattgcgat atctgtccct
      301 gcaccgattg ccatcccgat tgaagcacgg gctaaacttg gcgcatcatt gatcccatca
      361 cccaccatga ttactttttt cccttggtca aggtatttct ggacaacagc ttctttatca
      421 tctggtaata aaccgccata atattcttcg atccctaaat aatctgctac tgcttgggca
      481 gcgtttttat tgtcaccagt aagcatgaca ggagtgattt ttctttcttt caatgttcgg
      541 ataaattctt ttgcttctgt ttttactacg tcacctaaag caacgattgc agctagatga
      601 ttttctaaaa tcaggaagct gatggtattt ccttgttctt ggtaaggttt caataatgct
      661 tgttctacgt ttaatccgag acgctcaact tctttttcat taactatttt cacttgttgt
      721 tttttgactg ttgcctctag cccgactcct gaaagatttt ttagattttc agcttgataa
      781 ggcttgattt catgttcttt caaataattc ataatgccaa cagccaatgg atgattggca
      841 ttcgcttcca aggctcctag atattgaagg atttcatttt tattgaacgc atcatcaagt
      901 acttcgacac ccgttacagt aaattttcct tctgttagtg ttcctgtttt gtccagcatg
      961 atcacatcta gatcattggc ttgctccagt gcattgcggt ttttcaaaag taatccgttt
     1021 ttagctgcta ttgatgtgga gcgagcaaca actaatggga tagctaaacc tagagcatga
     1081 ggacaggcga tgataaagac tgttaccata cgttctaatg cattaggaag atctgtcaag
     1141 aacaaccagg cgatgaatgc caggataccc acaattaatg cgacataaaa cagccatttt
     1201 gccactttgt cagataatga ctctagcttg gatttttctc cttgtgcttt tcgcaccatt
     1261 tccataacct tcgctagata accattttca ccagttccag ttacttcgat ttcgatcgtt
     1321 ccatccccat tgatagaacc gccaatcact ttgtcattgg cttgtttttt gattcctctt
     1381 gattcgccag tcacagctga ttcgtcaaca attgtttcac ctttcaagat tacaccatct
     1441 gttggcattt tatcaccaga ccggacgatc aaacgatcac cttcgtttac ttctttcaat
     1501 gaaactgtct cttcttttcc atctttcgtc aagcgtttca ctgattctgg cagtaattct
     1561 gctaattttt gtaaagcatt gctggcattc gacacagcat tcatttcaat ccagtgacct
     1621 agtagcataa taacgatcaa agtagccaat tcccagaaga aatccatgac gtgtgtatgt
     1681 ggattgagca gatttgcgat aaacgaatag acactataaa tatatgcaac tgtaatcccc
     1741 atcgcgatca gtgtcatcat agcaggactt ttttgtttca attccatctt agctccactt
     1801 aaaaatggct gaccgccata gataaatagt atagttgcta agatcaatac gagccaatca
     1861 gaaccaggaa acgttacttg aaatggaaat tccattccca tcattggtga aaaaagaatg
     1921 attgggatag ctaggataag cgatagccaa aatttttgtt tgaaattccc catgtgcatg
     1981 gaatgatcca tgccgccgtg ttcgtgatcc atgtgcatcg aatggtccat ttgtttgctg
     2041 tttgctatat ctccatggtt atgtcccatg tgcatggaat gatccatctg actatggtca
     2101 tgtttcatct ccatctgttt ttcatgagat tctgtacttt ctttgtttac tacttctttt
     2161 ttatgctcat gttttttctc catatcgttc ccattcattt ttactccatc ccttctctta
     2221 ctttttcatg tgtcgattta agctcaggga gttcaacaat acactgacgg aactaaacgc
     2281 catcgctcct cctgcaatga ttggattcaa aaagcctagt gcggcaaaag gaataccaat
     2341 cgtattatag acaaaagccc agaaaagatt ttgtttgatt tttctcaatg tcaacctaga
     2401 caactcgatt gtttgtgcaa tggatgccaa actgctattc atcagcgtca catcggctgt
     2461 ttccatcgca atgtccgttc cacttcccat tgccataccg acatcagcta atgctaaggc
     2521 cggtgcatca ttgattccat cgcctgccat accgactttt ttgccatctt tctgaagttt
     2581 ttctacgtag gctgctttat cctctggaag tacttcagca aagatatgct ttggatcaat
     2641 accaacttgt ttaccaatcg tttcggctgc cagtttgttg tctcctgtca gcataaaaac
     2701 atctaatcct ttattctgga gctgcttgat tgcttgtttg gcttctagtt tgatttgatc
     2761 agccacggca atcaacccga tcacttgctt ttcatcagct aaaaacatga ctgtttttcc
     2821 ctgctgttct aattctaacg cgtgttcttg gtattcatca aatgataggt tcaattcaat
     2881 taatcgttta cgtgtacccg caaaatagcg tactccatta atcgtaccgc tgatcccagc
     2941 acctgggtgg gctgtaaaat caatcatatc gtatgttgct gcttcttgca gttttccgta
     3001 ttccacgatt gctttaccta gtggatgttc agaagcttgt tctaaagtat aaaaaataga
     3061 taggacttct ttagaaccga ctagatctgt aactttgggc tttccttctg taatcgttcc
     3121 agttttatcc aaaacaatgc tgtctaagtg agcagctgct tctaatgctt ctccgccttt
     3181 aattaaaatt ccatttcgtg caccaacacc cgttcctacc ataatagctg ttggagtcgc
     3241 taaacctaat gcacatggac acgcaatgac taatacagag acactatgga tcaatgcgag
     3301 ttgccaatca ccagtgatca aacctgtagc tatgagcgtg ataaacgcga tgactaatac
     3361 gatcggaaca aaaatccctg agatccgatc tgcgattttt tggattggtg ctttgctccc
     3421 ttgagcatct tccaccattt gaatgatttg cgctaatact gtctgcttgc cgacttgtgt
     3481 gacttccatg tgtagcagac cattcgtatt gattgtcccg ccaaaaagcg cgtcatccgg
     3541 attcttttct acaggaagat tttcaccagt aagcatactt tcatctatcg cactgcttcc
     3601 tgaaataatt cgaccatctg ctggaatctg ctcaccagga cggatgacta gttgatcacc
     3661 aacgactact tcttcaatcg ctaacgtttc ttctttcccg tttcttatca cttgggcggt
     3721 ttttgtttga agagacatca attgtttgat tgcattacct gttttcgttt ttgctgtatg
     3781 ttccaaatat ttgccgagca aaatcagcgt gatgatcata ctgctgcttt caaaataaag
     3841 atcttggggg tgtccacgga aaaatccgtt gtaaatgctc aatgcaaagg cagccgatgt
     3901 tccaattgct accaatacat ccatatttgg tgctttcgtc ttgattgcat ggtacgcacc
     3961 tttataaaaa cgagcaccga cataaaattg gacaggagca gacagcacta actggacgat
     4021 tggaaaatga aaaaagtgaa caatcgccgc atggcttcct aacatcatag caatcattga
     4081 caacatcaac ggcaaagtca aaaccgtact caatataaga tcgaatagca tttttttcaa
     4141 atacgcttgc ttttcttctg caattttttg tttgtgggct tcatcgtaca aaattgcacc
     4201 atagccgatg ttttctacgc tttggattaa tttttcagtg gtcgtccctt catatttgac
     4261 acttgctttt tcagtggcca agttgactgt ggcattgacc acaccaggtt gtccgttcag
     4321 ctctttttcg attctagcag aacaatttgc acaagtcata cctgtaatga cgaacgtatc
     4381 cattttggtt tctgctgttg ccatcaaatc acctctgctt tataacccaa tccattgatt
     4441 gcttgacaaa tcttttcgga ttgaactttt gtttcatcaa acttgaccgt accgtttgct
     4501 tttttcaaat ttactttcac tttttgcacg ccgtcaagtg cgcttactgt ttcttctact
     4561 cttgctgcac aatgattgca gctcatacct tcgattgaaa attgttgttt cattttttct
     4621 tcctcctatt gatgttgttt acattcacat tgacctggga tacagttaca ttcgatcgta
     4681 tctactggtt gcttttgttc cagttgtttc aaaatctcat tgatatcttc ttgagtcaaa
     4741 gtggcttctt ctactagatc cgcaatcgtc tctccaatcc tttttgcaca gatatgggaa
     4801 aaaagatttt ccgtcgcact gcgaactgtt tccatctccg agacagctgg atgataaatg
     4861 aatttttttc cttcttgttc tgtccagatc acttcttttt ttaccaaacg tcccagcaaa
     4921 gttttgatcg ttgctggttt ccaatccatc gaatcagcta agatctgcgt gatttcttgg
     4981 gctgtcgtct gtccaagtgt ccaaataaca cgcatgatct cccactctga atcactgatc
     5041 tttactggcg ttttgacttt ttccatttat ttcacacctt tcgtttacaa atgtaatcaa
     5101 taacttatgg atacagatta cacttgtaaa caaaaactgt caattatttt tgctctctat
     5161 tttcaattta gttaaaaatt cttgtttttc ccctcttgtt ttaaggataa tcagtttgtt
     5221 ttgtgcatgg ttttcttgga tcagctgctg tatttcgggt tcatgggttt cttcaaaatg
     5281 ccagatgaaa gacagaaatt ccagatattc cttatcgaat ctttctgaaa atgtctcagg
     5341 catatctggt cgggtcttct tgtttatctt tcttttgagg gaacgtaaaa tcactcgata
     5401 aatcgcccgg caacgcggaa cttgtagcca aatgatctta tctgcttctt taatcctctt
     5461 tcctagtgta gaagtatagt ttccttcaat gatccatgaa tgattagtct ccataaatga
     5521 ttgctgttgt tgtatgaacc acttttttgc ttggatgctg taatctgtcg tatgccataa
     5581 aaggtctagc ggcagtagcg gaaagccggt gatttctttg atcttccttg cgagggtcga
     5641 ttttcccgac ccactttgtc caatcacgat gatcttcatt cttctccctc acttctctta
     5701 tcattttgat ttgatcatac cacttataac gccgcaaaaa aacaagggac tgtgacaaaa
     5761 gtcgtgtcaa agtcccttgt ttcgaataaa tggtgttcaa tcagctgacc ttcagcatta
     5821 gctcaaaaag cggcaaaata tgaggagcta tttttccgtt tgtttctcta atactcaggt
     5881 caaatcgctg attttacaac ctcttcctta aacggtgttc aaaagcttgc atctgcggta
     5941 ataagcccag ccaaacgaaa aatatgaggg gctatttttg tttggctgtt cttattactt
     6001 gatgcaggac agctttttca caacctctga ttcacggtgt tccagaagta gcttcttcga
     6061 aaaacaagcc gaaacttcac aaaaatttga gaagcaattt tcgtacattc cttcttattt
     6121 cttgaagctg agaccccttc tgtcacaacc tcttgtaaca agtatttcta ttttaaggct
     6181 gaaccactgt ttctggttca gtgatttctt tgtttttgtc tcgacgagca attgctggca
     6241 agatcatacc taataagatc aagacgattg gtgtgatgat attcgtgaat aattggaacc
     6301 accatgcttt aggatcagca gtataatcta cttttggcac cattcctagg atacatgcaa
     6361 acgcagtgaa ggcaaagcac caagcaccaa agataaaacc gatttttgga tttttaacaa
     6421 atttatattc agaattgaat ttcttataag ctttattcaa catcatatat gccaagaaca
     6481 cccataggta acgcattggc atgacaactg agttcaagtt cgtcaaccat tttactaatt
     6541 cattcatatc tttgatacca aagatcggca acaaaataat gattcctact agtatacctg
     6601 tcagaagata accattcacc aatgtacctt tacgattacg tttacgtagc cagtttggta
     6661 caaattctgg atccgcatct gctaacaaga tttttagtgg tgcatcaatt gaaaaggcta
     6721 atgcggagat ttgtccaatc atgtttgtca atgcgtaaat gatcatcaat gagttaccga
     6781 taccatagta attacctaaa atttgaaaag cactgtatgc cccatttgcc atcaagtctt
     6841 taggaatatt atcgctagca aacagcatac ccattgcgac tgagcctaat acagcacaaa
     6901 tccctaccat tgctgctaag aagatcatcc ctttaggaaa ttcctttgca ggatttcttg
     6961 tagagttgac ataaggggat attttttccg ctcctccaac tgcaaatact agcatcgaaa
     7021 cggttgtgaa ataactgaaa tcaaatgttg gaatatatgt tttcacttga ttcatatgtg
     7081 gtgtggcaaa ttccatcgtt ggattgatga acggtgccgt tacagctaaa agaataaata
     7141 agatcgacat gacgaacatc gctgttccgg ccagtccacc aatcactttc aaagtagaaa
     7201 gtccttttgt tgataaatat aaaaaggcaa gaaatataat aagtgaaata actgctacca
     7261 tttgtactga catcgtattt acgatatttc catttccttg gactgcccaa ccgccagcaa
     7321 tcaggattgc ttgaggcttt tgtgccaaat aaggaatatg gacgacccag taagtccaag
     7381 cggcatagta tgccagtcgt ttattagatg tcgtattttc gacccatgaa ctgacgcctc
     7441 ctttactatc cttgaaggct gatcctaatt gtccaacgat caatgcgtat ggaataaaat
     7501 aaatggccaa aatcaatagc caagaggtga caactgatac tccttgctga gcatagttat
     7561 taactacatt ccccatgccc caaaccatgt taaatgcaat taacgcaaca gtaaaccagc
     7621 gtaattgttt gttgttcatt cgttctctcc tttatgataa aaatttgtta tccagatgta
     7681 atcatattca atttttaaca aacttacaag acagtgttat tttatgt
//
LOCUS       DACDUU010000103         7397 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.103, whole genome shotgun sequence.
ACCESSION   DACDUU010000103 DACDUU010000000
VERSION     DACDUU010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.103"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..142)
                     /locus_tag="IWL30_002617"
     CDS             complement(<1..142)
                     /locus_tag="IWL30_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9707327.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQ"
     gene            complement(183..437)
                     /locus_tag="IWL30_002618"
     CDS             complement(183..437)
                     /locus_tag="IWL30_002618"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9707328.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(548..802)
                     /locus_tag="IWL30_002619"
     CDS             complement(548..802)
                     /locus_tag="IWL30_002619"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9707329.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(995..1270)
                     /locus_tag="IWL30_002620"
     CDS             complement(995..1270)
                     /locus_tag="IWL30_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAP9707330.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1242..2195)
                     /locus_tag="IWL30_002621"
     CDS             complement(1242..2195)
                     /locus_tag="IWL30_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9707331.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2807..4300
                     /locus_tag="IWL30_002622"
     CDS             2807..4300
                     /locus_tag="IWL30_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAP9707332.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4435..4731
                     /locus_tag="IWL30_002623"
     CDS             4435..4731
                     /locus_tag="IWL30_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP9707333.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            complement(4755..5714)
                     /locus_tag="IWL30_002624"
     CDS             complement(4755..5714)
                     /locus_tag="IWL30_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9707334.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHFLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIR
                     CSARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFK
                     SITFDCGKEFSNWKQISNANDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDESFIQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
     gene            5897..6748
                     /locus_tag="IWL30_002625"
     CDS             5897..6748
                     /locus_tag="IWL30_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707335.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            6769..6981
                     /locus_tag="IWL30_002626"
     CDS             6769..6981
                     /locus_tag="IWL30_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707336.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         2442 a         1199 c         1330 g         2426 t
ORIGIN      
        1 attgctttac ccatttgtca aatgctgaag ccgttagttc atattctcga atgatttcta
       61 cacgtggctt tccagctaag taaagattga cgatttgttg cttgaattct tgtgaaaaag
      121 ttcttcgtgt tctcttagac ataaaaattc ctcctggtat gttttcttct agtctacaca
      181 ccttaatttt tctgtctagt taattgtagc ctatccatga tcggtttatt agacctttga
      241 ggtctatatt ttttaactac aatcgacctt aaatttaatt gtttcattag cttttggaca
      301 tgtttaagag atactgaaat cccttctttt aataaaactt gatggatctt tgtggcacca
      361 tagcgttgtt tattcgtaaa aaaaattgtt gaaatcttct ttttcaactt attatttctg
      421 atttctgtag ctgtcaacgg tttattctta taaaaataat agacacttct agggatttct
      481 aataaccgac acaacaaaga aacagtgtaa tccttacacc atttttcaat aaattgaaca
      541 agcgaattta ggttttccga gagaatatgg ctgcggcttt ttttaagatt tcaagttcct
      601 catttaaacg agcattttct tttctcagtt cagctacttc ttttccagtc agtcctgtgg
      661 actgatcagg taaatataaa ttcttccatt tgtaaatcgt tgcttcagat aagccatatt
      721 ctttcgctaa ccctcgaaca gaacgtccag tttgattcag ttcaacaatc atttgtttaa
      781 aattttcttc atatcgggtc ataaaaattc atcctctcgt agataagttt aatttattct
      841 tgtctctacg aaaagctgtc taagatttga tactaacacc aaatgttgta tacccccaaa
      901 caatacacat attttgaagg attaggatta ataaagctaa aaatatagta agtatactaa
      961 atatatttac tatatctagt atacttacta tattttaatc ttttgatcgt ctattatata
     1021 taatccgttc ctcgtcgttt aaagtatcaa cataggcgtc cattgcaagt ttgactattt
     1081 catacatagg aatatctttt gcataactaa gatcccgaat ggtttttagt acgggaggat
     1141 ctacttgaac tggttttctt tctttcgcag tgtactttcg ttcccctaac tcgacattaa
     1201 tatcatcttt aattgaaata tcttcagcac caattttctt cttatctttg ttcgttggta
     1261 ataatgccat ttagtttaat ctcctttcca agaggtagga tatgattggc cattgagtca
     1321 gcatatttag gtgtataatc aaatccttca atatcaccat gttgttcata gtattcaatt
     1381 ctttcttcta attctgtaaa gatgtctgca aaaatagccc accatcttcg atctacataa
     1441 tcatttaatt ggataccatt ctcaccatac atttctaacc gatccgagcc tttaacaatg
     1501 cttttaaaaa tgtttgattc accatactta tcaattgttt tttgatagtt tgcgtgttga
     1561 gaggaacgtc gcactgagaa aagaacaggc aaaattccaa tgatttgaag atgagactct
     1621 tcaaaattcg tgacaatcgg aactaaaact ttattaataa aatcttctgt tccttccatt
     1681 gcgtaccgtt ttaattcttg cattgcaata atataatctg cagcagttaa gaatgcacga
     1741 accacattat ctgttgatgg tgggcagtcg ataaagatat aatcgaaatt ttttctcaat
     1801 tcttctaaag gtttaatgaa ggccaatgat ctcttacgac gatcaggata tttttcggac
     1861 aagtattcat taagttcttc agatccagga gagccagcca taatatataa atgtggactt
     1921 acttgagtaa tacctgaaac ccagtcccct tttttgattg ctgcagacat ggatcgatca
     1981 aagctagtaa tttgatacgt tgatccaatg ttggaagtag tatttctttg ttggtcataa
     2041 tcaattagca acacgtcttt ttcaaaaagt tgactagaaa caatcgctaa taaatcattg
     2101 tctgtagtct taccaatgcc gccttttcta cctgtaagca ttacaacttt tcctagttta
     2161 tctgatgttt taaatgcagt ccgtaagttt tccatagttc cctccaattt cctatactaa
     2221 atatatttag tatacttcat atacttaata tattcgatat atctaatata ctccaaattg
     2281 ataagaattc agactttata aggaatatag tggatatact aaatatattt gctatattca
     2341 gtatatcaga tatattcaaa atgtcaaaat atatttgtgc gttttgcgtt gacaatttag
     2401 agtgaaaaga ggtaaactta attctataga aaccacagat aattcactat ttacaaagta
     2461 gcaaaagcag tggtactctt taaacagaaa aattacataa aaaaaagaga cggaaataat
     2521 tgattttagc agaatcaatt atttctgaat cacatggtta ggacatgtgt tcacgctcgt
     2581 ctcaagaata tgtaagtagc taccaactac ttatattaaa agttgatcgg ataccttttc
     2641 cgattgcctt tattctacca aaaaaaggcg agcaactcaa cagaagaacg tttatttagt
     2701 gtacttaaat taaatattct taaacactga agagagaaca catactatgg ctgagattaa
     2761 agctaaactg tgttctcttt ttgtttgaga aaggacgtta caatcgatgt tgagcgaaat
     2821 ttattcagct attcttaaaa acggcttacg tacatacaaa tatcgaaaca gcaccatgaa
     2881 acctgcaggt catattgatt ccagtaggag tggtgctatc tttgcgtacc gtagcaagca
     2941 attaatgaat gttgggcgtg gtatggtcat tacttcggaa gaagctattt tagagaacga
     3001 aaagaaacta acgcattgga cacctaatac ctatcgcttt ggtacttatg cggatgatta
     3061 tcgaacagtg gttaaaggtc attctgagaa aaacttaagt caaatcaaca catttgtagt
     3121 tgatatcgat tcaaaagaga accatcaagg cgaaattatt ttagcctgtt tagatcaagt
     3181 gggctacatg ccaacgctaa ttttagaatc agatcatggt tatcaagttt attttgtact
     3241 aaagacacca ggctatgtca caaagaaaag caatttcaag gtgatcgatg tagcaaaaaa
     3301 aatttcaaaa acaattcgtc tacagttggc tgaaaaattg tccggagtgg atctaggatg
     3361 taaccacttc ggaattgctc gattcccaca agaaaataat attgttttta aagaaatgaa
     3421 ctatcagtat tcttttgctg actggattca atggtcgatg aagcttgaat cagatcaaaa
     3481 aacaaaagaa gatcgagaac gaaaactaat tgtctttcct gagaaaaaag aaattcgtca
     3541 agtagatgaa ccgtggttcg atatgttact tcataaagca aacatcgtcg gcggagaagg
     3601 acgtcttggt cgaaataatg tgattttcac gctttcttta gcttattact cttcaggtta
     3661 tgcattggat acttgcgaat acaatatgtt cacattcaac gaacggttac aggaaccgct
     3721 atctgaagca gaattagtaa agattgtacg tagtgcttat tctggtaatt atcaagcagc
     3781 aagcagagaa ttcattattg aattgtgcca gcaatgggtt tcaccaacct taaaaaataa
     3841 tgatctgttc attcaacgac gtggctggtg gaaatttaaa aagccacgag atcaaagaaa
     3901 atatagtcat aaaaatgaat ggaaagaaga tctattggct tatctaagcg aaaaaagtct
     3961 tgctagtgag ccttatttga acctgtcaaa aaaagaattg gcttcacaac taacgatgcc
     4021 caagcgaaca cttgataaga tcttaaatga gttacgagag gagaataaag tcttctacaa
     4081 ggtaacacga ggaagaaatg gcggaatgat cattgcttca gtcaaaacgt tgttcgcgag
     4141 ggttattcaa ctaagtaaag aaaagagact agattattca acaaggatca cagaggtatt
     4201 tggactcaac gagagcttag tagaagagac atttagacaa ttatcaaccc ccttgaaccc
     4261 tcttaaagaa gtgaatcttt ttgaagtaga tacaggttga aaaataaaag gtgcaaattg
     4321 ccttatacaa tatatctatg atacttctgt aggtgttgct ccctttgcct agctacgtgt
     4381 aggtggtata cttataactg cgtatgcagt cttatgtatc atggaggaag ttgtatgtcg
     4441 ttaaaaaatt atgtctattc acatcctgtt aatgtgttta tcattggtcg tttgggatta
     4501 tctgtggaag tgttctgtga gctttatggt tttaagcagg ggactttgtc ttcttgggtt
     4561 accagggaaa aaacagttgc ttctttgccg attgagtttc ttcatgggtt gtccttagct
     4621 tctggggaga ctatggatca ggtatatgat tgtttgtgtg tgttagagca ggagtatatt
     4681 gagtataaaa tagctaacga gttaagaaaa aggaagaagt atatccagta gtagttttta
     4741 tttactctta attgtcaaat taagttagac aaatcatcaa tacttacgta actcaaaaat
     4801 acttccaaag gtgttcgata attcagtgat tttctaggaa tattatttcg tttcgatgcg
     4861 acagattgaa taaaagattc atctactgaa ttgaaatcca tagatttcaa taaaccatct
     4921 ctacgtaaca atccgttaga attctcgttt aggcctcttt gagacggcgt tcctggatca
     4981 gcgaaataaa tggcaatatc attggcatta ctgatctgtt tccaatttga aaattccttt
     5041 ccacaatcaa aagtgatgga tttgaataga tttttcggta cagattcaaa ccaatgattt
     5101 aatttgtttt caatatcaat cgcttgtcta ccacacggtt tcaatgtgat gataactttt
     5161 gataatcgct caactaaggt aattacagca cttttatgtt tcaaacctac gatagtgtca
     5221 ccttcaaggt gaccaaactc atttgagaat tggctataat ctttttcacg ttcatgaata
     5281 gagcgacgga aagcttgttt tccacgtctt tcttgatgtc catttggttt acgcttgcct
     5341 ttcatcggta agtcagaagg attaaatagc ccctttttga acatacgata aatggtacga
     5401 gcagaacaac gaataggaaa cgctgcacga ccaacaatca catcgggtgt ccatccttga
     5461 acaacctttc tttgaatata ttctgattgt tcctcaggta aaacaagcgg gcgtctacca
     5521 cagtttgatt tgtttttctt atactgttga tagtactcta aggctgattt tccttgcttg
     5581 aagaacaggt atactttatg gatcgtttgt cttgaacgat tcaagaaatg cgcagttttc
     5641 gcaacagatt gatttatttt gaaataagac tctatcatta caagttcatc cgttgtaaga
     5701 tgggtatagg tcatttgtac tcactcctta taattctttg gacggaacta ttttgagtgt
     5761 agcacaaatg acttttttag ttgtctagct taattttaca atcggcgtac ttaaaaaatt
     5821 ggttcattga gcaacctatc gctgctaaag tagcaaagga aaacgttaaa ggaaagaatt
     5881 tagggaagaa atcgaaatga aatcggacga ctggattgaa cacacctggc ttcctctgaa
     5941 aagaaatagc aaacgaaaga aattttgggt attgcaatta tggccattag gaaaactttg
     6001 tagttacggg gcacgactta ttcttgattt aaccgtttac tcgattggag tatttggaac
     6061 ggcttattac ttgataaatg atcccaatag gattgcagca tcagacattt ttacagtgct
     6121 aatgacgttg atttttgtca tgctattgtc tatttttact caattagtca tgcaatttac
     6181 caggcaaaaa ttaacagcag gaaaactttt caaaagccta ttcgatttaa ttgttgtaaa
     6241 tgcaattcta gcgactttaa taaaacaaat tgaacaatct ggaagaacaa caaaaggagt
     6301 gaaggatttt ttattgacgt ttattcactt tgtaaatgga cacatgtatt tgtttgaagt
     6361 gggtattatt ctcttttttg tcgggtatgt atccacgaaa cttattaaac gttcaattga
     6421 aaaagaaatt gaaattgacc tgattccgac acttcttggt tatgagtaca atgttgattt
     6481 tggaagatat gacgaattga tcaatacgga tatcgttcac acgagtatcc atgaatctac
     6541 tgttaagggc atcagatcta ttttaaaaaa tggccaggaa atgaaagcaa tagatcgaag
     6601 aaaagagatc tacaattttt atgaagtcga agcaagccta cgatatggca tcggaaaatt
     6661 gtggttagat ggaagtaata ttcgtcaaaa caaaccatac attttttgga acagtagtga
     6721 tttagaactt ccaatgaaag ataaataatt atggagaatc gtttgaaaat gattcctcta
     6781 tacaggattg tgcttatagt tggcatggag ttaaaaacct taaagaagga gcagttattc
     6841 aaagtgaaag aacgtcataa tcagttacta tgtagtaaaa cgggtacgat gaacattgaa
     6901 taccgaacag aatatttaga attaacgaat caaactgatt ttttgttgat tcgaaagcaa
     6961 caagagcaag aaaaaaatta atggctactt atagccaatt gattgatgaa tgggaacaat
     7021 tcaaggaatc taaaattcca gaacgtctct aaaagccttt atttgtaaag agcataagga
     7081 aacgtgggaa cactttttaa aaaaagtgtt ccaataggct gtgcctatta cgtttctcaa
     7141 aaataaatgt gggaacaaaa tatttaaaaa taggatagtg aaaggatagt gaaggatagt
     7201 aaaaggaggg tagtaggata gcaaaaagga taggctcctt ttcgctatcc tttttaaatt
     7261 ggaggaatta atatggaagt aagaataaga aatgtggatg agaaaatcgt aagagaaatt
     7321 gatcgaagat gtgacgaacg gttctgttgc aaagttttaa atctactatc aaataaggta
     7381 gaataataga aaaagat
//
LOCUS       DACDUU010000104         6935 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.104, whole genome shotgun sequence.
ACCESSION   DACDUU010000104 DACDUU010000000
VERSION     DACDUU010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.104"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(1..1151)
                     /locus_tag="IWL30_002627"
                     /pseudo
     CDS             complement(1..1151)
                     /locus_tag="IWL30_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1394..1849
                     /gene="tnpA"
                     /locus_tag="IWL30_002628"
     CDS             1394..1849
                     /gene="tnpA"
                     /locus_tag="IWL30_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAP9707337.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            2114..2212
                     /locus_tag="IWL30_002629"
                     /pseudo
     CDS             2114..2212
                     /locus_tag="IWL30_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2435..2665)
                     /locus_tag="IWL30_002630"
     CDS             complement(2435..2665)
                     /locus_tag="IWL30_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAP9707338.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2895..3713)
                     /locus_tag="IWL30_002631"
     CDS             complement(2895..3713)
                     /locus_tag="IWL30_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9707339.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3874..4563
                     /locus_tag="IWL30_002632"
     CDS             3874..4563
                     /locus_tag="IWL30_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP9707340.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4577..6079
                     /locus_tag="IWL30_002633"
     CDS             4577..6079
                     /locus_tag="IWL30_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9707341.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            6092..6568
                     /locus_tag="IWL30_002634"
     CDS             6092..6568
                     /locus_tag="IWL30_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP9707342.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6665..6775)
                     /locus_tag="IWL30_002635"
                     /pseudo
     CDS             complement(6665..6775)
                     /locus_tag="IWL30_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2252 a         1092 c         1265 g         2326 t
ORIGIN      
        1 ccaaccttga tctgagtgaa acgtacgacg atagggacaa tcgttagtgg tctggatagc
       61 ttcctccaaa ccttccatta ccgctaatgc attgggcttt tcagagatac gataggataa
      121 aatctcacta ttgaacatat ccataaaagg atctagatac aatttctttt gacggataat
      181 gcctttggca tccacttcat ggtatttaaa ttcagttgta tccgtcgtga ttttctgatg
      241 gcagatactg gtgtagaaac gacgatggat ccgatttttg gctactgctc cgacttgtcc
      301 tcgataagaa ttgtagcgcc gtgatttacg tgtgtatgtc gttactttta ttcctagctt
      361 cttgatcagg cgctgaactt tctttttatt gatagagaaa ccgcggtttc ttagttcatt
      421 ggtcatccgt aaacagccat aatccttgtg cttctcccga actttaagca tttccgcttc
      481 aatctcttga tctggatttt tacggtccaa tcgtttttgc cagtacataa agattgcctt
      541 agggaacttt aaagtttcta gaagctcaac taatttaaac ggtcctcgga ggcttgcgat
      601 gattcgtgcg attgcttcat tcgtcgttgt tgggcttcct gttttcgcag cttcctcaat
      661 tcttttaaaa aggcattttc aatttgaagg gaacggactc gtttctccaa ctcctcgatg
      721 cgttcacgtt ctttagacgt tgctttagtt gtagattttt tcttattttt gggttcattt
      781 ttcttagaca agataggtgg acatcctttc gtttttgaga gtccatcgat cccaccctca
      841 cggaattgac gcatccaact agcgatcaaa ttagggttgt tcatgttaag tgtgttggca
      901 acttcacgat aggacagctc acttgttaga tataactcta tcgcatccag cttaaattga
      961 acagaatatt ttttcttttg tcgctttctt actagaccct cttcaccaaa ttcttgatag
     1021 gcattgatcc agcgcttcac ttgtgatgga gccttaatgg aatatttctt tgccagataa
     1081 tcacttccgc ctttaccatc caagtaatca tgaacgatct ttaatttaag ttcaaaagta
     1141 tacttagcca taaaaaatac cgacctccaa aagttagatt ttttggtcta acttttgggg
     1201 gtcggtacaa aacttaaaag ttttagaccc ttttattttc ttaatgactt cagtcagttt
     1261 tgtgcgtaaa gcgtgcgaaa caagaaaacc gcgcgtttct acgcgtggaa gaagtatctt
     1321 tagcgataaa aactcctttc gatatactag tgagtggcta ccaaccgctc atcagatcga
     1381 aaggagtttt ttcatgtcaa atgacgataa aagtttagca tatactcgtt ggaattgtaa
     1441 gtatcatatt atttttacac caaaatatca acgaaaagta atctatgggc aattacgaaa
     1501 agatcttgga aaaatactta ggaagttgtg cgaaatgaaa gatgtggaaa tcatagaagc
     1561 acatgcaatg ctagatcata ttcacatgct tgttcgagta ccaccaaaaa tatgtatatc
     1621 cagttttatg gggtatctga aaggtcgtag tgcggtacta atccacgagc agcacgcaaa
     1681 tctcaaatat aaatacggaa acaaatcctt ttggtcgaag agatactatg tgagtacagt
     1741 cggattgaat caaaagacaa atgaaaaata catccgtgaa caagaagctg atgatcgcat
     1801 tcgatatatt ataagtaaaa gagagtacgt agaaccattt aaaaaataaa gagaagtaag
     1861 tgagtgaaga tgagcagttg gcggtctttt aaaaatcccc ttttaagggg cagcgagtgt
     1921 cgaccccttc tagggggatt aaaaatacac ccgtcctcac gggtgtatgg gttactgata
     1981 gacttgattt taactggtgt cagaaactta tgtccagaaa gggtgatgaa attttggcaa
     2041 tttacggcta ttcaagaatt agcacaagca accaagatta caaaacacaa attcaaacat
     2101 tagaaaatgc tggcgcagaa ataatcttcc tagaaaatca ctggattatc gaacaccttt
     2161 ggaagtattt ttgagttacg taagtattga tgatctgtct aacttaattt gacaattaag
     2221 agtatattta cttcagatgg taaggcaaca aaataaaata caataggcag atgaactaat
     2281 aaataaaatc tgtattaaat acagataaat taatataaaa atcaaaagga tcataaactt
     2341 ttaacaagtg atgaaatggg tggaaattga ttcttataga cagtttgtct cataacattg
     2401 aagtattttg caattttagc attgaaacac ttttttaatc ttcaacgttg ttcttataaa
     2461 caagatgatt gggaatcttt tgaattaaca ctgtatagct tagaacaccc tttatagaaa
     2521 acattcgctt agcaatctta ataattcctt gtgcccgatg tcgtttagat tcagtaggtt
     2581 cctgctcatc gatcatggaa aaaatttgaa ggatcagatc cttaataaat ttatttaaga
     2641 gaggatgatc cattgcttca tccatactcg ataaactagt gataatgagc tacacgtttt
     2701 tctttcaagt tgatcaatag cttccacgat aaaaatatct ttagataaaa tcaagaaccg
     2761 taggaactac agggatattt ggtcaataag agccaccgct attagtaaaa aataagctat
     2821 caagtatgct ctcatcccaa gaattgccta cttcaatcgt taaaaaccgt taggtttagc
     2881 taagtggggg tagttcactc aatactttta tcaataatag agcgcaaaac tttttctctt
     2941 aattcaactg aatcaatatt attgttttgt tcataaccat gaactaatag atcacaaata
     3001 taaagttgtg atatttttcc agctaaagat ccaccattaa gaaattcttc aatggctgtt
     3061 tgtaaaacta agtctgcaga ctttcctata ggtgaatgaa tatagttagt tatagcaata
     3121 atttttgcgc catttttttt agctattttc aatgaatcat aagtatcttt cgttttccca
     3181 gataaagaaa aaatgattac taaatcatta actgttaata aagaagcgac ttgggcttga
     3241 aaatgagggt ctagtacagc ttttgcttga actcctacgc gaagaaacat attttctaaa
     3301 tcaagactag tatttccact agatcccact ccaaatatat atatgttttt tgattgagtg
     3361 atcattttaa tggcttcatt caatttttct tcgtctaaaa gcctaatagt ggaatagaga
     3421 gcttctgtta aactattagc aatttcatca tagtacttta cactagtcgg atgttctttt
     3481 ttttgactaa agtcttcctt tgcaatttca atttttaaat cagaaaaacc agaaaaacca
     3541 attttttgac aaaagcgaat aatagtagca tcgccaactt ttgtgttttt cttaatatca
     3601 ctcatcgtac tataaataat agttttccca gaatttaaaa taaaatcagc gacttttttt
     3661 tcagacttcg ttaaaaatgg ataattttct ttaattaaat agatataact catacaaact
     3721 cctctacaat ttcaactttt ctccattata ctccaaaata ataaaaaaaa ctcctaaata
     3781 ctccattttt atctttactt ttatgaaagc ggatgctata ttgtttttgg agttgttatc
     3841 caaaaataat aaaaaattgg agtgtttagg ataatggata aaaagaggtt tttagatctt
     3901 atttcaaaag gtttgattgt ttcctgtcaa gcattacctg gtgaaccatt ttacaaagag
     3961 gaagaaagtt taatgcctta ttttgctttg gctgctcaaa gggctggagc agttggtata
     4021 cgtgcaaatt ctgttcgaga tattattgaa attagaaagt gtgtatcatt accaatgatt
     4081 ggaattatta aaagagatta tccaccagaa tctccattca ttactgccac tatgagagaa
     4141 gtagatgaat tagtagaaac cggtgttgag gtaatcgctc ttgattgcac tcttagaaat
     4201 cgacatgatg gactatctat aaattctttt attactaaaa taaaagaaaa atatcctcat
     4261 cagttattta tggcagatat ttctacattt gaagaaggca aaaatgcata taaagcagga
     4321 atagattttg taggaactac tttaagtgga tatactgagg aaagtaaaaa acaagatggg
     4381 ccagatatag atttgattaa aaaattggta gaagaaggga tttgtgtaat tgctgaggga
     4441 aggattcata ctccagaaca agcaaaagag ataaaaaaaa taggagttgc aggtattgtt
     4501 gtgggtggtg ctattactcg accacaggaa attgcatcaa gatttgttaa agcattaagt
     4561 tagggggaga aataaaatgt ttaaaaaatt ttcacaatta ggacgcgctt tcatgttacc
     4621 aattgctata ttaccagtag ccggattact attgggactg ggaggagcgt taacaaatga
     4681 gagtgctata aatgcttatc catttttaga tcaaccgtgg ttgcatacaa ttttaagtat
     4741 tatgagttat gctggaaatg cagtttttgc aaacctagca ctaatttttg caattggtat
     4801 tgctgtagga cttgcaaatg gagataaagg aacagctggt ttagctggtg gagtagctta
     4861 tctagtatat actgcaacta ttagtgggtt tcttgaactt ttttcagcaa aagatgcaac
     4921 actagataca ggtgtagtgg gatcaattgt tgttggtggt actgttgcat ttttacacaa
     4981 tcgttataga aaaattgaat taccacaatt cttaggtttt tttggaggat caagatttat
     5041 tccgattgtt tctagtttag ctgcaatcat tattggttct ttcttttatt taatttggcc
     5101 accaattcaa aatggtttag tagttgctgg tgaacaaatt gcccaaatgg gtagtttagg
     5161 aactttttta tatggttttt tattgcgttt aacaggtgct gttggactac accacaccat
     5221 ttaccctttg ttctggtata catctttagg tggtaccgaa actgtagctg gatcaacaat
     5281 tgctggagcc caaaatattt tctttgccca actagctgat ccaaaccaca ctggtctgtt
     5341 tacttatggt actagatttt ttgcaggaag atttgcaaca atgatgtttg gattaccagc
     5401 agcatgttat gcaatgtatc gtgcaattcc taagaaaaat cgtaaaaaga atggtgggct
     5461 ttattttagc ggggccctta catcattttt aactggaatt actgaaccag ttgaatatat
     5521 gtttttattt gttgctcctt ggctttatgt gattcatgca ttcttagatg gattatcttt
     5581 ttattttgct gatatcttaa atattcgtgt agggaattcc ttttcaggtg gtttgatcga
     5641 ctttttatta ttcggtgtac ttcaagggaa tgacaaaact aattggttaa aattaattcc
     5701 ttttggtatt gcatgggcat ttatatattt ctttgttttt acattcttta tcaaaaaatt
     5761 taaggtagct attcctggaa tggaagaaga atcagaagtt cattctgaaa aagtaataaa
     5821 caaaaataca tcattacatg aagagtctct tatcattatt gaagctcttg gtggaaaaga
     5881 aaatattgag tctgttgaag cttgtgcaac tcgtttgcgt gttgctgtta cgaatgggga
     5941 gattattcaa aaacaaacaa taaaagacac tggtgcaact gctgtcttcg aagtgaaggg
     6001 tggcatccaa gctgtttttg gagggaaagc tgacttatta agtcaagaaa tcaatcaaat
     6061 attaggaaaa gataattagg aggtttttaa tatgtttgaa attttcaaaa agaaaaaaca
     6121 agaaactttt tatgcacctt gttccggaaa aatcatttcc atcacagaag taaatgatcc
     6181 tgtcttttca caaaaattac taggtgatgg ttttgccgtc atacctgatg gttcaaaaat
     6241 ttatgctcca ttggctggtc gagtaacaaa tatatttcct actaaacatg ctattaatat
     6301 aaaaagcaac tcaggcattg attatttgat tcatattgga attgatacag ttgaattaca
     6361 aggagagcct ttttcgattt gtgttcaaga aaatgatgag gttactcctg aaacctcgtt
     6421 ggttgatgtg gattttgaca aaataataaa atctggtaaa gatacttctg ttatagttgt
     6481 ttttccggag aaaaatcagt tcaatgattt aataatagat aatggtcgaa ttgctcatgg
     6541 gaattcatgt ggaaaaatca ctttttaaaa aagtgtatat aaaaagctat aaaataagct
     6601 aaataggata tttgtcaaat gaagttggtg aacagaaaga actgttttct ttctgttcat
     6661 ttttttattt tattagtaat gattccccat gccaaatcat gatccgtaat ttaaagttat
     6721 aaaagcttcg aaatccattg gcatttcgtt ggaatacctt gatatgatta ttcaaatata
     6781 aaatatagag agaagagaga gagggaagat atttataaag ggcaaagtta tgtttactca
     6841 acttaggaaa tagaaataga tcaatttata ttctcttgtc aatcgaatat gaaccttagc
     6901 actatatacc aaatatagta aaatgctgaa agatg
//
LOCUS       DACDUU010000105         6585 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.105, whole genome shotgun sequence.
ACCESSION   DACDUU010000105 DACDUU010000000
VERSION     DACDUU010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.105"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            242..2473
                     /gene="pflB"
                     /locus_tag="IWL30_002636"
     CDS             242..2473
                     /gene="pflB"
                     /locus_tag="IWL30_002636"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP9707343.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            2717..3478
                     /gene="pflA"
                     /locus_tag="IWL30_002637"
     CDS             2717..3478
                     /gene="pflA"
                     /locus_tag="IWL30_002637"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAP9707344.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            3563..4489
                     /locus_tag="IWL30_002638"
     CDS             3563..4489
                     /locus_tag="IWL30_002638"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAP9707345.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            4671..5564
                     /locus_tag="IWL30_002639"
     CDS             4671..5564
                     /locus_tag="IWL30_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAP9707346.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            5698..5823
                     /locus_tag="IWL30_002640"
     CDS             5698..5823
                     /locus_tag="IWL30_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9707347.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(5957..6583)
                     /locus_tag="IWL30_002641"
     CDS             complement(5957..6583)
                     /locus_tag="IWL30_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707348.1"
                     /translation="MQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWLLL
                     DHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSFLA
                     ILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSLFS
                     FFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
BASE COUNT         2385 a         1099 c         1300 g         1801 t
ORIGIN      
        1 attaatacag gtggcgtctc tttttcattc atttttaata aatccttata cgtttgcaaa
       61 aaaagatgaa agtgttcaca atgcttaaat gttggtatgt caacgttgat ttaaatatat
      121 gtaaataaaa cataaaaatt gttaaataaa aaacaagaaa acccttgcaa agatagggtg
      181 ctgtgttata ctaatcacgt aaaaactgtt acataaaaca gaaatttagg aggtccatac
      241 aatggagcaa tggaatggat ttaaaggtac caaatggaaa gaaggcgtag acactagaga
      301 ctttattcaa aataactaca ctgaatatcg cggtgatgac agctttttgg aaggcccaac
      361 agaagcaaca gaaaaattat gggatgaatt gcaaaagtta aatgatatcc aattcgagcg
      421 taacggtgtc tatgatatgg acacagaaat cgtatcaact attacttcac acgatgcagg
      481 ctatttggat aaagatttgg aaaaaatcgt cggtctacag acagacaaac ctttgaaaca
      541 agcattcatg ccgttcggcg gaatcaatat ggctaacaat gcgctaaaaa gcaatggcta
      601 tacaccaaac gatgaattga cacatatttt cactgactgg cgcaaaacac ataatcaagg
      661 tgtatttgac gcttatactg ctgaaatgcg tgctgctcgt aaaaacaaaa tcattacagg
      721 gcttccagac gcttacggtc gcggacgtat catcggtgac tatcgtcgtg tggcattgta
      781 tggaatcgac tatttgatgg aacaaaagaa aaaagatcat gatatgactg gttatcatac
      841 aatgagtgaa gaagtgattc gtttacgtga agaaatcact gaacaatatc gtgcattagc
      901 gcaaatgaaa gaaatggctg cgaaatatgg ctatgatatt tctaaaccag ctgcaaatgc
      961 aaaagaagct gtccaatggc tatacttcgg atatttagca gcaatcaaat cacaaaatgg
     1021 tgctgcgatg tcaatcggac gtatctcagc attccttgat atctatatcc aacgtgattt
     1081 ggataatgga gtaatcacag aaaaagaagc acaagaattg atcgaccact tagtaatgaa
     1141 attacgtatg gtcaaattcg cgcgtacacc tgaatataac caattattct caggattccc
     1201 tatctgggct actttatcca ttgcaggtat gggattagat ggtcgttcat tagttacaaa
     1261 gaatgatttc cgtctgcttc atacattaac aaatatggga ccttcaccag aaccaaactt
     1321 gactgttttg tattctgaac accttccaga aggattcaga acatatgcta gcagaatcgc
     1381 tatcgaaagt tcttcgattc aattcgaaaa tgataatcag cttcgtgaac aatggggttc
     1441 tgatgattgt gcgattgctt gctgtgtatc aggaacagtt gtcggaaaag acatgcaatt
     1501 ctttggcgct cgtgctaact tggctaaaac actattatat gccatcaatg gcggagtcga
     1561 tgaagtgact ggcgcccaag ttggaccaga atatcgtcca atcacatcag aaatactaga
     1621 ctttgaagaa gtgaaacata acttcatgaa tatgatggat tggttagcag aactatatgt
     1681 caatacgttg aacatcattc attatatgca tgataaatat tcttatgaag cagctcagct
     1741 ggctttgatg gattctgact tgcaacgcac atttgctaca ggtattgctg ggatctctca
     1801 tgcggcagac agcttgtcag cgatcaaata tgcaaaagta aaagcaatcc gtaatgaaaa
     1861 aggaatagca gttgattttg aagtggatgg cgacttccct aaatacggaa atgatgatga
     1921 ccgtgcagat gaattagttt catggatctt ggaatacttc atgggacaaa tcaaacgtca
     1981 taaaacttac cgcaactcaa aaccaacaac ttcattattg acgattactt caaatgttgt
     2041 ttacggtaaa aatacaggaa atactcctga tggccgtgaa gctggaaaac cacttgcgcc
     2101 aggagcaaat ccaagttaca atgcggaaca aagtgggtta ttagctagcc tgaactcaac
     2161 agctaaaatg aattacggct gtgcattgga tggaatttca aatacccaaa caatcaatcc
     2221 ttcaggtctt ggaaaagaac aagatacaag aatcgaaaac ttgcgtaatg tcttagatgg
     2281 atacttctca aaaggagctt atcatttgaa tgtcaatgta ttcagtaatg atttattgcg
     2341 tgatgcaatg gaaaatccaa tgaaatatcc gaacttgacg atccgggtat ctggttatgc
     2401 agttaaattc cgtgacttga caagagaaca acaattagat gtcttgatga gaacttcaca
     2461 cgacagaatg taaaaagttc gtgaaaaaag tttttcgact tagcgtcgaa aggacgttga
     2521 atagccatcg catcaaaatc gaataatcag tatgcttatt tgaaagaaac gatttacagg
     2581 ggcaggactt gtatgttccg tccctgtaaa ttttacacat aacgaagcgg caattgcgaa
     2641 aaattttgtt cagaaagctt cgcttcgaca gctaacttgt acttaaaatg aaatcaaaga
     2701 aaagatgtga acttaaatgg aagaaaaaac aatcggctat gttcattcta tcgaaacttt
     2761 cggttcagta gacggacctg gactgcgctt tgtcgtgttt atgcaaggat gccgcatgcg
     2821 ttgccaattt tgtcacaatc cagatacttg gaatattggc ggagggaaag aatacacagc
     2881 agatgaactt ttagataaag cagaacgctt cagaccttac tggggagata aaggtggtat
     2941 tactgttagt ggtggagaac ctctattgca gatcgatttt ttgatcgaac tatttaaaaa
     3001 agcaaaagag cggaagatgc atactacatt ggatacatgc ggaaaacctt ttacgtatga
     3061 agaacctttc ttttcacgtt ttcaagaatt gatgaagtat acagatttac tgttatttga
     3121 catcaaacac atcgataatg aagagcataa aaaactaacg cattgggaca atgaaaatat
     3181 tttagaaatg gctcaatatt tgtcaaaaat caacaaaccg gtttggattc gccatgtact
     3241 agttcctgag cgaagtgatt atgatgaata tttgatccga ctagacaact ttattcaaac
     3301 cttgtcaaat gtagacagag tagagattct tccttatcat acgatgggaa aatacaagtg
     3361 ggaaacactt ggtttgaaat atccattaga agggatcgaa ccacctacaa aagaacgtgt
     3421 cgaaaatgct aaacgattgc ttcatacaga agagtataca aaatatttaa cgcgataggt
     3481 cgcatcggaa agaagataag tattgcttct ttccgttttt ttgctattat taaataggag
     3541 aaatcacaaa ggagcgaatg acatgtcaaa aattttagtt tttggtcacc aaaatccaga
     3601 tacagatgca atcggggcag ccattgcgtt ttcacacttg caaaatgaat taggtaaaga
     3661 aacagaagca gttgctctag gtacacctaa tgaagaaaca caatacgcat tagaccactt
     3721 tcagctatct gcaccacgag tagtagagtc agctaaagca gaaaatgcag atgtcatgct
     3781 tgtggatcat aatgaattcc aacaaagtat ttcagatatc agcgaagtaa atattttagc
     3841 tgtagtagac caccatcgta ttgcaaactt tgaaacagct aatccattgt actatcgtgc
     3901 agaaccagtt ggatgtacaa gtacgattat tttaaaacta tataaagaaa accaagtaac
     3961 aattcctaaa gaaatcgcag gaatcatgct ctcagctatc atttcagata cattgttgtt
     4021 caaatcacca acatgtacgc ctgaagatga agcagctgca aaagaacttg cagcaatcgc
     4081 tgaagtcgat ctaaacactt atggacttga aatgttgaaa gcaggaacaa atctaagtga
     4141 taaatcagct gcggttcttc ttgatttgga tgcaaaaagt ttcccaatgg gcagttccaa
     4201 cgttcgtatc gctcaagtca atacagtgga ccttaatgaa gtgatggatc gacaagcaga
     4261 gctagaagca gcaatggaag atgaaatgaa agcaaatggc tacgatctgt tcgtattagt
     4321 tgtcacaaac atcttagaca gtgattcaga attacttgtt gctggtaaag gaaaagaaaa
     4381 agtagaagaa gctttccaaa caactttgac caacaaccgt gctttgctta aaggtgtcgt
     4441 ttcacgtaaa aaacaagtcg tacctcaatt gacggctgta tttagctaat caaatggtag
     4501 tatgaagcat tcaaaatgaa tggataagcg attgatataa aaagaaacat agaacatact
     4561 aaaggccgaa gaaatggagg agaataacag tcaattatga ctcttgttct tcctcctttt
     4621 tacgtaaaag ccttttaagt aaaataaaac ggatagaaaa gagaacatat atgaactatt
     4681 attcaaaaaa taaggaaaac ttttatcaaa aactgaccga tgatccgctt ttttcttcat
     4741 tgaccgatta tctatatgaa catcgagaac aagaaacgat tctaagagaa ttgaaaaaag
     4801 aattttcgca aaataaattt tcccattttc ttgatttatt gatcgatgca ggattaatca
     4861 agagggaaga acgaagatat catttgaact ttccaatatt tgatcctaaa gattacttgc
     4921 aacaagccac ttcggctgct gaaaccatag ctgaacaatt gaagagactc tctgttgatg
     4981 aacaaaaatt agcgatggga gaagttattt gggcgtattg tttcgaagat gaacggaaag
     5041 aagcgtattt ttatggagta agaaacagcc gagagacaga attgctgagg acaactgcag
     5101 gaaatgagaa gtaccgtttt atcacacttt cctctataga acacttcccg cttactttgg
     5161 ctaattactt ttttgtacag aagaatcagc ttcctgtaac gaaggcattc aaagaattag
     5221 ccgaacttat tggagacgta aatgaatcgt acttttttga tcaaatagaa gttatcgttg
     5281 acaggatacg taagaataaa tataaaaatc gccggcctag tattttccat cagtctctac
     5341 ttgtgacgaa tactataaag gaagaagaat cattcacatt agaattacct attgtcgaaa
     5401 aaaacaattt tgagattgaa ttaccaacat tggatccttc attgacaatg gaggaaatgg
     5461 cctttcttaa acgacaaatc ttttctgagc tgagcaagaa atttataccg catgcatttt
     5521 cttatatcaa agaatatgga acagttttag tgagtaaaac ctaagagagc agtaagcaaa
     5581 aattatactg actcttgcag gaggagcctt tctcttcctt tatggttaaa agaattattt
     5641 gagtattttg cctatctacc acctattaga ttcactacat ggtctggaaa caaaggaatg
     5701 caaaaaaatg gtgagaaatg tggaatgaca aaggaagtag tgataagaaa agttagattt
     5761 ttaaataatc aatattatga ttcagtcaag tatggcattt tatgggaaga actagctgat
     5821 tagattagga agtccatttg ataatccaga atcactaaga aacgaatggc gagtcattaa
     5881 taatttatcc aatcttgggt tattctttta gattgaagtg tttaaaacta aaaaagcatt
     5941 tatgaagtcg taatagtcag gcttcaaaat gctttttatt ttgtttgttt tttctttgcc
     6001 acataaatac acctagactg acgatgaaga gcatatccaa cagatttcct gcgacttgga
     6061 tcgtcgaagg gttggtcagt ggcattaaag caaggacaaa aaaggaaaac aagctgtaaa
     6121 taaaacaggt gacaggaact aatcgaaaga agagcggaca tttcaattgg ctaaaaatga
     6181 gtgaaaaaat aaaataagta aaaatggata aaatcacgac taaaccattc atgataaaga
     6241 agaaaagcgt attgtgatta aacgaataaa aaagaattgc taaaaaagaa aacgttttaa
     6301 atgcaatcaa tacgcttgaa gcttcgttta acgtaccagg atgtcgatga aaaaaatagg
     6361 caccgaaata aacagaaaac agtaagccgc aaagagaaga caaaatcaaa aaaaacggat
     6421 tttcttcggc ttctgtgata tcagaatgat ccaaaagcaa ccagcctaat gcactagaag
     6481 gcgcatgtga ggtcttaagg tatgaataac atagaaaaaa taagatcgtc aaagttgcaa
     6541 aaaaagagcc taaatgtaaa aaattagtgg ttctacgttg aattt
//
LOCUS       DACDUU010000106         6460 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.106, whole genome shotgun sequence.
ACCESSION   DACDUU010000106 DACDUU010000000
VERSION     DACDUU010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.106"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(5..697)
                     /locus_tag="IWL30_002642"
     CDS             complement(5..697)
                     /locus_tag="IWL30_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9707349.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRSGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            692..958
                     /locus_tag="IWL30_002643"
     CDS             692..958
                     /locus_tag="IWL30_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9707350.1"
                     /translation="MHRTFGSRGCPTLHLADRYAERKTPTLCFAFSARTRQAGIPLED
                     IKDFLGHKDVSTTQVYAHISPEVKKRSMNQLENYIEEQIKKHSN"
     gene            complement(1134..1703)
                     /locus_tag="IWL30_002644"
     CDS             complement(1134..1703)
                     /locus_tag="IWL30_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9707351.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1777..2100)
                     /locus_tag="IWL30_002645"
     CDS             complement(1777..2100)
                     /locus_tag="IWL30_002645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707352.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVITDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2241..3065)
                     /locus_tag="IWL30_002646"
     CDS             complement(2241..3065)
                     /locus_tag="IWL30_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707353.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFLHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKYFTKNGFVT"
     gene            3419..4267
                     /locus_tag="IWL30_002647"
     CDS             3419..4267
                     /locus_tag="IWL30_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAP9707354.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4412..5352
                     /locus_tag="IWL30_002648"
                     /pseudo
     CDS             4412..5352
                     /locus_tag="IWL30_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
     gene            5342..6073
                     /locus_tag="IWL30_002649"
     CDS             5342..6073
                     /locus_tag="IWL30_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707355.1"
                     /translation="MTIKREKWLRGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSILWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYWYAPFLSLMAYFMMLTVFRKKDLLYEMIAV
                     VTVLFVYLAARQFFSIRLLSGNTRFQSLTTVLLICFLTFSAILPLYLPKVHWNGNSFF
                     DKFYKVQNFFPYAIPIVLDNEPPRM"
     gene            complement(6103..>6460)
                     /locus_tag="IWL30_002650"
     CDS             complement(6103..>6460)
                     /locus_tag="IWL30_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9707356.1"
                     /translation="TEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVA
                     ESTYKSLKVEFVYQYTFETLQQLDSELFDYVNWWNHLRLHGTLGYETPVGYRNQRLAQ
                     RILDNELGCANASEAV"
BASE COUNT         1844 a         1280 c         1160 g         2176 t
ORIGIN      
        1 ttttttatcg ttgtggaata tttaaatacc aacgcttgtc atgaaaatct tgtgctccac
       61 ccttagtatt tccttctgga tcgtttgtcg cacgcatcat gacgtatact ttcttattag
      121 ggaaatcacg catattgaaa gatacatgat agccaacatt tcctaaagta ttataagctt
      181 gatttacgtc tggtctataa atcccatcag ctcttactct agctagttct tttccagtat
      241 tgtagtccat aatgaaaata tactcgtatt tataattagc aatgtgccat ccagctacat
      301 gcaagtttgc gttttcgatt tctccgaact gatcaatgtg ggcgtaattt gttccatctg
      361 tcagcgtaga atttgcagca cctgatcgag ttggatcaat tactggttta tcctccgaag
      421 tagttggatt atcatccgta aaaccatgag ccaaatcata ggctagtttt tctttactta
      481 cacccatttc agaaagataa ccgtaaggat ctgtatgatc gccccaaata ttttgtgtta
      541 cccataaatg cgatttgatt cctggttggt tataaggagt gtctaatgtt aatggaatac
      601 catatttcat tgctgaatct cttgccaatt caacgtatac tttatagttc ttttcaaaag
      661 tttctttatc atgtgtgtgt tgtaactcga tttgcacagg acttttggct cacgaggttg
      721 tcctactcta catcttgctg atagatatgc tgaaaggaaa acaccaacat tatgttttgc
      781 tttttcagct agaacgagac aagcaggaat acctttagaa gatatcaaag attttttagg
      841 tcataaagat gtttccacta cacaagtcta tgcccatatt tctcccgaag tgaaaaaaag
      901 atcaatgaat caactagaaa actatataga agagcaaata aaaaagcact caaattgagt
      961 gcttttttaa gctcactatc actaaaacta tcactaattt tatttataag cgaacatgtt
     1021 tctatcactg attttttaaa tctagcgaaa aacccttatc ttaaaggcgt tcgttcaatt
     1081 ctttggcaag gtcttcaaag cctggcttgc ctagaagtgc gaacatggtt ttcttatagc
     1141 tcatgtagaa aaattttctt gatgatgatt ttatccatca attgagattg actgttttgc
     1201 tctcctatga tttcagctat gattgtccga ttttgcggtt ttcttcttaa ctggtagttg
     1261 attttcccag tttccatcaa ctcgctaagc atatccacga ctccgccaaa tccggtcttt
     1321 ttcgtaataa aacctagtgc taccattcgt tcatctagat tttttgctgt aatcgtcaca
     1381 ccagtaatga gcgagtgctg taatgtttgg gccagctctt ccatcccttc agcattggat
     1441 acgacggaag ctgttttgtt aataggttct tcgtttattt cttttggttc tttcctctcc
     1501 actttcactg tttgacgaag gacatctagt tcttgcaaga gcgtttcgta ttgttcttgg
     1561 gtcagcatca cgccagctac tttttcacga ttaaatatat atacgccagt tcccgcgtct
     1621 cgcgcttgag caaatacatc catcggcgat cgtttcactt ctgtgatcga agatgtggga
     1681 acttctaatt tctttaattc caatggattt cctcctttac taatcgcttt ttattatagc
     1741 acagactgac ttgttttgat cttttttcca aaggttttat cggtttttgc ctaaccagta
     1801 attgatatcg gtaagaagtc tttcgatacc aaatgaagga tggtacagat acgttttttg
     1861 atcttcgtta tggtctagtg gaaaaaaatg atcggtgatc actagatctg cataccgagg
     1921 cggcttattt cgagtacata catgtataga gatccgtttt cgatgtctta gcgttttgtt
     1981 cagtacccca gcaagtcttc ttgcttcttg gtgttcatag cgaaaaaaaa agacgatttt
     2041 cacttcagga gggtggacat ctaaataccc tcgttcctgg agtgttttca gtaaaagcat
     2101 cccccactct actgcggggt gtgcaagtgc ttgttggatc gctagccgaa attgatatag
     2161 tttatctttg gaattgggaa atttttctaa tgtttcctga taataacgaa cttcttctat
     2221 cagccggtca tggataccaa ttacgtaacg aagccattct tggtaaagta ctttgtcagc
     2281 tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc aagcttttca
     2341 gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca ccgcttttca
     2401 gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt tattggcaaa
     2461 atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt tcgactgtct
     2521 cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat gaacatctca
     2581 gaaaaggact gctctccatc atctgcaaaa aaatgcagaa aatctttcaa aaagcaaaac
     2641 agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata agaaaaagaa
     2701 aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc gtacttctgc
     2761 tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga ttggatcaga
     2821 aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg aacggttttc
     2881 ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag atgccgatcc
     2941 actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca gatcaaatct
     3001 ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag tttttgccat
     3061 ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat gggttcactc
     3121 ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc ctcattattt
     3181 tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt aaaaaaacat
     3241 aataagatca ctttgcttgt aggatgtatt cccttccatt acaatgaaat aaatagcgat
     3301 tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac aaaaaatcga
     3361 tcagttgata taagaattag aaaaagcggt catcggcaaa cgttcaacta tccaattgat
     3421 gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg gtgtaggtaa
     3481 aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca gagtccaatg
     3541 tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt cacaatcaaa
     3601 agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttggcag atgagatcaa
     3661 tcgtacgact cctcgtactc agtcagcact tttagaagca atggctgaga atcatgcaac
     3721 gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta cgcaaaatcc
     3781 aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt ttttattccg
     3841 actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg ataaattcga
     3901 aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag aaataaaaac
     3961 aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc agttagtgtc
     4021 agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa gcctttcctt
     4081 tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca cgcccaaaga
     4141 tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg atcgttcatt
     4201 gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc ctattcctgt
     4261 gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat tttgtgttat
     4321 ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct ttaggctggt
     4381 tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta agcagctttc
     4441 taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa aaaagtatgc
     4501 aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct cgtttacaaa
     4561 tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta caaataaacc
     4621 gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa cattaactgt
     4681 catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa gagagatctc
     4741 ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat tacagagagt
     4801 tctggaaaaa aacagcagct gaacgcgtac caaagaggtc ttgattttcg ggaacatcgc
     4861 gcttatcgtc caggtgattc tttcaaccga atcgattgga aattgtctgc tcatactcaa
     4921 gagtggttat accgtgaata cgattatcaa gaagaagaat gttctcctct tttttgcttt
     4981 tggggttcgc tcagtccaaa atttgaacct ctgctagact tgtatttttc tttatggcat
     5041 ttgagaaaag aaaagcagcc ggaacttctg atattaggca accgcgcctt tcatggcaaa
     5101 gaccccggtc atacctattt tgcttctttg gaagctggcg atgagaaagc ttttttcgca
     5161 cacttgaaaa aatatgcaaa aaaacaaatc gtcctctttg ttcctgagaa ttctcctgaa
     5221 gtctcagaag ctattgaaac attgtcaaaa gatcgaacgg tttatcttgt ttatttcgta
     5281 gaaaaacaac tcatggtcca agaaaaagac aagatatgtt cattgccagg aggtgaatgg
     5341 attgacgatt aaacgggaaa aatggctgcg tggcgtttac tcctttgtga tactaagtgt
     5401 aggtgctccg atatttgtag tagctaatca attgcaaaac agctaccttc ttatcctaac
     5461 tattggtgtc ctatgcctta ttgtctgctg tattcctaaa aaatgggtac gtttgctgct
     5521 ttctttgcca gtaatgatag gttgtttgtt tttatatttt ccttcgaaga cattttccat
     5581 attatggatc ttacagtttt tttcgtcagt cacagaggaa ttcttccatc tatcccaagg
     5641 taaactttat tatttgccag aaaagacagc tttttttctc ataatgttgc tgatctattt
     5701 atttattaca ttgaccgtgg attacgacta ttggtacgca ccctttcttt ctttgatggc
     5761 ttacttcatg atgcttaccg tttttcggaa aaaagattta ctgtacgaaa tgattgcggt
     5821 agtaactgtt ttgttcgtgt atctggcagc aaggcaattt ttctccatcc ggttgttgtc
     5881 gggaaacact cgattccagt cattgactac agttctgctt atttgtttct taacttttag
     5941 cgctatcttg cctttatatc ttccgaaagt acactggaat ggcaacagtt tttttgacaa
     6001 attttataag gtgcagaact tctttccgta tgctattccg attgtccttg acaatgagcc
     6061 tcctcggatg tgatgatctt cggcaggagc taacagttaa agttagactg cctcgctagc
     6121 gttagcacat ccgagctcat tatcaaggat tcgctgcgcc aatctctggt tacggtaacc
     6181 aaccggtgtc tcgtagccaa gtgtaccgtg caaccgaagg tggttccacc aattgacata
     6241 gtcaaataac tccgaatcca attgttgtaa ggtttcaaat gtgtattgat agacaaattc
     6301 tactttcaac gacttataag ttgattcagc tacggcatta tcaaaaggac agcctttatg
     6361 actcaatgat cgattgatgt caaaagttgt taataattca tcaatagctt ggttatcaaa
     6421 ctcttttcca cgatcagtat gaaaaatctc aacctctgtc
//
LOCUS       DACDUU010000107         6250 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.107, whole genome shotgun sequence.
ACCESSION   DACDUU010000107 DACDUU010000000
VERSION     DACDUU010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.107"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(368..1075)
                     /locus_tag="IWL30_002651"
     CDS             complement(368..1075)
                     /locus_tag="IWL30_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707357.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENG"
     gene            complement(1140..1976)
                     /locus_tag="IWL30_002652"
     CDS             complement(1140..1976)
                     /locus_tag="IWL30_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9707358.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(1994..3403)
                     /locus_tag="IWL30_002653"
     CDS             complement(1994..3403)
                     /locus_tag="IWL30_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9707359.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3417..5240)
                     /locus_tag="IWL30_002654"
     CDS             complement(3417..5240)
                     /locus_tag="IWL30_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9707360.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
BASE COUNT         1929 a         1338 c          998 g         1985 t
ORIGIN      
        1 aaacaattac atatgatttt ttcataaaaa aacagtactg tctagtagta taaaatatac
       61 aaatttctta aaaatttttt cactaaaatc actacctgaa acagtctaaa acacttatat
      121 atcaacgttt aatgaaacta tctccgtttc atttcagata caaataaata tagtcgggct
      181 ccaaatccga aaaaaaatac ccccagtagc ttatcctaag ctactggaga tgtataagta
      241 cggtcatata acaacggggt agggtaaata aatgaaaaag atggaagaat cattattcaa
      301 taactttttt aaatacttcg ttgtaatcag ttacctgtcc gttcttaaat accacagaat
      361 aatcttttta cccattttca agtaaccgaa gtggaatgtc tttaccaact tgctctgtca
      421 ctgcattact gatacctgtt agattagcta ataaatctga taaagattga gcatctgtag
      481 aaatctctgt atcagtattt atcgtgattc ctttatcttc atttacaagc ttccaagtag
      541 gtggtatagc tgtgtatgtt tcgatgaatg aatcaattct ttcatctatc gtcttcccag
      601 tatctacttc tgtctcatta ctcttagatg aatcgattct ttcatccatc gtctgctccg
      661 taactgcttc tgtctcatta ttctcaccgg aaactctctt cacacccaaa cttatatatt
      721 gtccatcttt agtgtgcata gttaaaattc ccacgctttt actaataaat tcactataac
      781 cagttctttc aatagagact tcaatgtatt tcacactatc atactcaaaa gaaaaataga
      841 catgtaaaag cattttgttg tttacatcta cataataatc ctttgactct acttgagacc
      901 ttcctatacg gaatacagct ttactgtctg atatctctat accagcatcc ttattattaa
      961 tctgccaatc tccaagtagc tcttctggca agctagtagt ttcttgtgaa cactttacca
     1021 cttcactggt agaggtcgaa acattcacct ccgcactgtt agaagaattt tccaaggaat
     1081 caggagcctt agcacaacca acaagtcttt atatattgtg cagccgatat tttcttcatt
     1141 cagtacaccc ttgaaatatg cagtgttaga taaagcagtt cgtcctgatt tgctgaggca
     1201 cccatctctt cttgaacgta ctccttgatt ttcaaggccg cccgatactc tttcggaaaa
     1261 cgctgcttca ctgaagccag caaaaaatca tccttctccg gcaactcttc cttcttaatc
     1321 attcgggcaa taaaaaactg taaatgcgtc acaaatctta agtaaagcat cgaatcttta
     1381 tcaacctttt tttcgggaaa cagatatcct agcatagcca agatatcctt tagtgctctt
     1441 accgagagca tggtttcttc catatttgct tgttcacttt gggcatttac aatgtgaaac
     1501 gcaatgtttc cagcctcctc ttctggcaac tgaacattca tttttttgtt gatcaattgg
     1561 atagcatagc agccgatctc aaattctttt gggtaatact tttgaatttc aatcagcagg
     1621 cgattttgaa aaacaatgcc tttttgactg cgttcaatgg catgcttcaa gtgatcaacc
     1681 aaggtaaaaa atagactttt attcagctta aagtcaaact tggcgttggc ataatcgata
     1741 attcgcttga cgcaaataat tagatcatcc ggagcagcct caaacagttt aatgtaatcc
     1801 ttttttagag catcagaaag tgaatctaat ttaaatactt tttggatttt gtcagccgga
     1861 accaaatcgc ctttctttag ttgaaatcca atccctttcc ccatcaaaat tacttctttg
     1921 ccttgcttat ctaagcaaag aattaaactg ttgtttaacg ttttaattac tctcattaga
     1981 ttcaccgttc tctctaagct aagccatttc ttgcgattac tttttgatac caaaagaagc
     2041 tttctttttt tattcgtttt aattctttaa gattctcatc ggttcggtca acatatataa
     2101 aaccataacg cttattgaag ccttgatggg tactaacaac atcaatcgct gaccaaggta
     2161 aataaccaaa aacatctaca ccatctgtta aagctgcttg aatagccaga atatgtttgg
     2221 ctagataatc aattcgatcc tggtcattaa ttcgtctatc ttcctcaact atgtcgggtg
     2281 ccccatagcc attttcagta attaaaatag gtaaatcata gcgatcgtac aatttcctca
     2341 atgttaatcg tagtccagta gggtcaacta cccagccata tggcgtttta tctacataag
     2401 gattttcagc tgcccggtag acaccttctt ctcccaacat gatctgttga tcccccgctc
     2461 tagcggaaac atctgatccg tcatttttgc tggcagcgat ggtggcagtt gaataataat
     2521 taatcgcaat aaaatcgggt ttaccggagg ataaaattgc ctgatcctct gcctcaattt
     2581 taggatataa tccacgatca ttcaaatagt tttcaaacaa ataattgtat tttccccgaa
     2641 ctgctacatc taagaaagac cagcctctca atgtttccca attatgggct gcaattgcat
     2701 cttctggact ggaggtagct tgatacatag ccgttaaatt gattgctgga ccgatttttc
     2761 ctttaagatt caactgatga tacaagtgaa ttactctagc ttgagctaat aaaacatggt
     2821 gattaatttg aaagagttct ttttttgaag gcagctctcc acttggtggc aaaccaatcg
     2881 ctccaggatg aaggatcatc gtgttttgct cattaattgt cagccaatag ttcaccttgt
     2941 caccatactc agtaagcaat acctgtacat agtccacaaa ataatcgata gttgctcgat
     3001 ttaaccaacc gccctgctcc tctagaaaat aaggtaaatc gaaatggtac atggtgacaa
     3061 tcggttcgat tccctgattc aggcattcat caattacttg atgataaaaa gaaattccct
     3121 tttgattggt tttattattt tcaatatcca taattcgcgt ccaagcgatg gaaaagcgaa
     3181 aagcttttaa gcctaattct ttaaacagct gtatatcttc tttatagtga tggtaaaaat
     3241 ccgaggcaac ttcaaaatta gctacccctt ctgtatgctg atacttgtca ataatagacg
     3301 gttgtttccc atcctcctga taggcaccct ctgcctggta ggcactaatg gaggcacccc
     3361 ataaaaactt ctctggaaat gctgaaagct gtttgtattg cacaaaaatc ctctccttaa
     3421 ctaatttgca tcaatggttc gtgttccttt aagcttccgc ttgcttcact aattcctgcg
     3481 taattttcac tgttggtcac taccacaatc actgtatcat cgtagccagc gtttctaatg
     3541 gcagcgaaat cggcgttgat taataattgt cctgctgaca cctgatctcc tgcttttaca
     3601 aatacttcaa agggttcccc ttttaaattc acggtgtcga tccccacatg gatcaataat
     3661 tcaatacctt ctttagaacg tagaccaatc gcatgtttag attctaaaat agtaatgact
     3721 tctccctcaa caggtgaata aatgctgttt tcttctggat gaatggctac gccctttccc
     3781 aatagtccct tagaaaagac agcatcgttc acttcagaaa taggacacac ggttcctttt
     3841 accggatttt caacaatgac tgcttcggct attgccgttt gctctgattc atcagaagta
     3901 tcgtcaaatc caattagata agcaatgata aaggttgcaa caaaggcgat tacacacgta
     3961 ataattgcgt gaacaatatt cattggattt tctccaataa aggctggtaa ggctgctaag
     4021 ccaggggaaa caaaggcata tctgactaaa ccgctcaagc cagcatacaa accagcaatt
     4081 gcaccaccaa tcatggaagc aactaatggt tttttcagct tcaaggttac tccatagagt
     4141 gaaggctcag taattcccat taaagcagtg atcccagtgg acacagctag ctgcttcaac
     4201 tctttgtttt tagttttcgc ggctacagca aaggtagcac ctgattgagc aatattagaa
     4261 gccagcattc ctggcccatt gatcatttca gaacccgtac tgctcaattg catagtagcg
     4321 attggcgtca ttgcccaagc tgtacccgta accaccaaaa atggctgcaa accacccatc
     4381 aaaaatggaa ttaaccaagc tgctttgccg ttaatccatt cagcccccat tgctataaga
     4441 tcgttcagca acgttccaaa cggtccaatg acaaccaacg ctaaaggccc cataattaaa
     4501 aataccagca tcggcttagt aaagaattta ataaacgaag gcgaaattcg atccgcaaag
     4561 ggctctacat aggacatgat ccaaacagta aagataatcg gtaaaactga tcccgcataa
     4621 tctgcagcta ataccggaat tcctagaaaa gaaacggcat cgccagtaga aaaaagctgt
     4681 gaaatatttg gatgaagcaa aatgccagca acagtaatcg ccaaaattgg gttgacatca
     4741 aactttatgg ctgccccata ggctaatagt actggcataa aataaaaggc tgcatcagca
     4801 atagtattca aaatgatgta agtttgacta ctgtctgata gtacaccagt caaagtcaag
     4861 acggccaaaa tggcttttac catcccggca cctgttatag ctggaatcat cggtgtaaag
     4921 gtggtagaaa tgacactaat caatcttgaa ataatacttt cttgattgtc tgaagagggg
     4981 ccctttttaa ttgaagttcc cacttccttt tgaatttcct tgaaggctgt ttggacttca
     5041 ttgccaatca ccacttggta ttgtccaccg ctatccacca cactaattac accaagtgtt
     5101 ttttctaagg ctttcgtatc ggctttcctt ttatctttga gttcaaaacg cagtctggtg
     5161 gcacaatggg ttaagctgac cacattttct tttcccccaa cctgttgcaa tacatttctt
     5221 cctacttctc tgtaatccat tattctctct ccttaaagca gtaaaaaaga ccaagtcaaa
     5281 ccacgcccca atcaacattg aagtgttagt ttgtcttggt cttgcctgca taatcagtaa
     5341 catgccaata acgttatttt gctgtaatcc aataatagca cattatgata gcgcaatcaa
     5401 cagggaattt aattttttat cttttcgttt gaccgtatag ggtaatataa cagttagatc
     5461 acatcaacta gggtatatct gcgtatactt actgctaaaa taagtatatc taatattaac
     5521 tataccagta aaaccattcc tacataacca gcgggaaatt cccaaaatcg tgaaagcatc
     5581 aaaaatgcat ataacggatc acacaacttc aacaattctg ctcttgggaa aaattgatga
     5641 atttggtgag taaaggatgg aataaacagc attccaacaa tataggcaac aatagtgata
     5701 cttgtttttt tggtaaaaat cagtaagcaa tagccaattt caatcattcc gagatataaa
     5761 ggtatcgata gaatgatttg ggctagtatt cttaaaaatt aactagcaaa tgtttaaact
     5821 gatttatttc taatctacat gtagtaagta tttcaattcc accaattatc aataaaaata
     5881 ccaaacatat ccataataat tttttctttt tcatttcttt tatattgtta taacagtgaa
     5941 taacataaaa attcccgaca atcattgtaa tatttttttg tcacctgtat tagtatttaa
     6001 tttataggaa actataaaaa ctccctagtg aatttacacc tttgggagtt cctgtttgga
     6061 cggtatgcat aatacattta aatcaattta acattaacac ctcagcatat ggtatctcca
     6121 catatatacg gtctttcttt ggatagtact caatatttat gttgatagca cttccattgt
     6181 ctaatatagc tttagcccgg tatgtgttat ctccattttc atgagtaatc gtcacatcat
     6241 cacatgatcg
//
LOCUS       DACDUU010000108         6246 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.108, whole genome shotgun sequence.
ACCESSION   DACDUU010000108 DACDUU010000000
VERSION     DACDUU010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.108"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..517)
                     /locus_tag="IWL30_002655"
     CDS             complement(<1..517)
                     /locus_tag="IWL30_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9707361.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGV"
     gene            complement(650..1333)
                     /gene="rpiA"
                     /locus_tag="IWL30_002656"
     CDS             complement(650..1333)
                     /gene="rpiA"
                     /locus_tag="IWL30_002656"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAP9707362.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            1652..2065
                     /gene="rpsL"
                     /locus_tag="IWL30_002657"
     CDS             1652..2065
                     /gene="rpsL"
                     /locus_tag="IWL30_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAP9707363.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            2149..2619
                     /gene="rpsG"
                     /locus_tag="IWL30_002658"
     CDS             2149..2619
                     /gene="rpsG"
                     /locus_tag="IWL30_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAP9707364.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            2713..4800
                     /gene="fusA"
                     /locus_tag="IWL30_002659"
     CDS             2713..4800
                     /gene="fusA"
                     /locus_tag="IWL30_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP9707365.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            4972..6159
                     /gene="tuf"
                     /locus_tag="IWL30_002660"
     CDS             4972..6159
                     /gene="tuf"
                     /locus_tag="IWL30_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP9707366.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
BASE COUNT         1899 a         1244 c         1287 g         1816 t
ORIGIN      
        1 aaacaccgca ttttgaagaa ataaattgga tcgtttcttt tttttcataa tagagacgtc
       61 ggattttttt gatcgttttt gcatcgattt ttggtcttcc gccgattttt ccttttttac
      121 gagcttcatc taatccaact aatgtacgtt ctttgatcaa tgcgcactcc atgtttgcta
      181 ggttttccat taattggaaa tagatttttc ccatttcgcg cgtatcgatt ccttcatcaa
      241 gactaatcaa gtgcagcccg cgatctttga ataattgcgt gagttctgtt aattgccgcg
      301 tagatttgcc taatcgatga agttggcaaa tgactagtgc atcgccttgt tgcatgtcag
      361 cgatcactga ctccaaatga acagcagtag aatcattttg ttccacttca tatgtctcat
      421 ggaaaatttc ttgacaacca aagtcagata attgatcaac ctgagttgtt aaatcaacgt
      481 catttatggt tgtgcgtgca taaccaataa ttttcatggt gattcctttc tagcagtgat
      541 tttctaactg gatataacag taaaaatgta gcataaagta cgtcaaaaag atagtttttt
      601 cgcaaatttt ccgctgtctt tttttattgg atgatcaagc agtttttctt tatttacgac
      661 ggattgcgtc aagaatctca acgccttttt ctgagccgac aactacttta gaaaccatgt
      721 ttaggaaaag cccatgttct actaccccaa caagttcatc taaataggta gctaagcttt
      781 ctggctgttc gatgacttct agatgcagat caatgatgta atgaccacca tctgtggtta
      841 agacttctcc gttttcgtct gtacgcaaga cgggttggaa tcttttttct tcaaaaatat
      901 gaagtagctg ttgggagcca taagggatca cttcaactgg gagtgggaac ttcccaagtt
      961 tatcgactaa tttagaagaa tcaacgatcc aaatcgtttc ttttgagtac gtagcgacta
     1021 ttttctcgaa aagcaaagca gcgccgccgc ctttgatccc ttgaaaatca gcactgattt
     1081 catcagcacc gtctattgtc aaatcgacta cggggacttc atcaatactc tttaacggta
     1141 ttcctaattt ttccgcttgt tcttttgtcg catttgaagt agtaactcct gtgatacgta
     1201 aaccttcttc acgcatacgg cgaccaatct cttctaccat ataataggcg gttgaaccgg
     1261 tcccgagacc tacgatcatc ccgtccttta cgaatttcgc ggcttcaatg ccagctaatt
     1321 gttttaaatt catctttgcg tccacctttt tttagtttga gcatcgtgct ttcctcacta
     1381 ttttagcgtg atttacggta aaaagatacc attctttttt attccaataa agctttttca
     1441 aaaaaaattg catctcttgt tgacatgttt tttcattcgt gatattctaa caaaggtgct
     1501 ttatctacag gtttctgtga agaaacgtgc tgactgttta tcaaagcata tttgataagg
     1561 atgcagacat tgcatttatt ttttatcaca aaaaaagaac cacctggatg tgtggaacta
     1621 gacgcaaatc gtgggaagga ggaaacaagg aatgcctaca attaaccaat tagtacgtaa
     1681 acctcgtaaa tcaaaggtgg aaaaatctaa ttcacctgct ttgaacaaag gatataacag
     1741 ctttaagaaa actcaaacta acgtaaactc acctcaaaaa cgtggggtag caactcgtgt
     1801 gggtacaatg acacctaaaa aaccgaactc agctttacgt aaatatgcac gtgttcgttt
     1861 gtcaaactta attgaagtta ctgcttatat cccagggatt ggtcataact tacaagaaca
     1921 cagtgttgta ttattacgcg gtggtcgtgt aaaagactta ccaggggtac gttaccatat
     1981 cgttcgcggt gctttagata ctgctggtgt taacgaccgt aaacaaagcc gttctaaata
     2041 cggaactaaa aaacctaaag cttaatttta attattaaac aaatgaaact tgcttctact
     2101 atatagaagt aattactatg aacaatattt cggaaggagg agttacggat gccacgtaaa
     2161 ggtcctgttg caaaacgtga tgttttacca gatcctattt ataactcaaa attagtaact
     2221 cgcttgatca accgtgtaat ggttgacgga aaacgcggga ttgcagctaa cattatctat
     2281 aatgcctttg acttgatcaa agaatctaca ggtaacgatc cattagaagt gtttgaacaa
     2341 gcaatgaaaa acgttcaacc tgttcttgaa gttaaagcac gtcgtgttgg gggttcaaac
     2401 taccaagtac cagttgaagt tcgccctgaa cgccgcgtta ctctaggtct tcgttgggta
     2461 gtaaactacg ctcgcttgcg cggtgaacac acaatggaag aacgcttagc aaaagaaatc
     2521 atggatgctg caaacaacac aggtgcttct gttaaaaaac gtgaagacac tcataaaatg
     2581 gctgatgcca accgtgcgtt tgctcactat cgctggtaag attctctctg tttggtcggc
     2641 ttttataagt cgacctcaga tagtgtcctt atttttagca actagatagt aaagagagga
     2701 gaaacaagga gaatgaccag agaattttct ttacaaaaca ctcgtaatat cggtatcatg
     2761 gcccacatcg atgctggtaa aacgactgct actgaacgta tcttgtacta tactggtaaa
     2821 atccataaaa ttggtgaaac tcatgaagga gcttcacaaa tggactggat ggaacaagaa
     2881 caagaacgtg gtattacgat cacttctgct gctactacag cacaatggaa gggtcaccga
     2941 atcaacatca tcgacacacc aggacacgta gacttcacag ttgaagttga acgttcattg
     3001 cgtgtacttg atggtgctgt aactgttctt gatgcacaat caggtgttga acctcaaact
     3061 gaaacagttt ggcgtcaagc aacaacttat ggtgtacctc gtatcgtatt tgcaaacaaa
     3121 atggacaaga tcggtgctga cttcttatat tcagtatcaa ctttacatga ccgtttgcaa
     3181 gcaaacgctc acccaatcca attaccaatt ggtgcggaag acgacttcac aggaattatc
     3241 gaccttgtaa caatgaaggc tgaaatgtat acaaacgatc ttggaacaga gatcgaagaa
     3301 acagaaattc ctgaagaata ccgcgaatta gctgaagaat ggcgcgaaaa attggttgaa
     3361 gctgtagctg aaactgatga agaacttact ttgaaatact tagaaggtga agaaatcact
     3421 gaagctgagt taaaagaagg tatccgccgc gcaacagtta acgtagaatt ctacccagta
     3481 ctatgtggtt cagcatttaa gaacaaaggg gttcaattac tattggacgc tgttcttgat
     3541 tacttgccat caccgttaga tatcccagct atcaaaggga ttgaccctaa aactgatgaa
     3601 gaagtagaac gtcctgcaga cgacagcgct cctttctcag ctttagcatt taaagttatg
     3661 actgacccat tcgtaggtcg tttaactttc ttccgtgtgt actctggtgt tttacaatca
     3721 ggttcatacg tacaaaacgc aacaaaaggc aaacgtgaac gtgttggtcg tatcctacag
     3781 atgcatgcca actctcgttc agaaatctct gaagtttacg ctggagatat cgctgctgct
     3841 gttggtttga aagacacaac tactggggac acactatgtg atgaaaagag tctagttatt
     3901 cttgaatcca tggaattccc agaaccagtt atccaagtcg ctattgaacc taaatcaaaa
     3961 gctgaccaag ataaaatggg tgtggcttta caaaaactat ctgaagaaga tcctactttc
     4021 cgtgctgaaa caaacgtgga aacaggcgaa acaatcatcg ctggtatggg tgaacttcac
     4081 ttagatatca tcgttgaccg tatgcgtcgt gaattcaaag ttgaagctaa cgtaggtgcg
     4141 cctcaagtat cttaccgtga aactttccgt gctggcactc aagccgaagg taagtttgta
     4201 cgtcagtctg gtggtaaagg tcaatacggt cacgtatgga ttgaatttac accaaatgaa
     4261 gaaggtgctg gtttcgaatt tgaaaatgcg atcgtcggtg gggttgttcc tcgtgaatac
     4321 attccagctg ttgaaactgg tttgaaagat gctatggaaa atggtgtctt agctggctat
     4381 ccattagtag atatcaaagc taaactttac gatggttcat accatgatgt cgattcaaat
     4441 gaaactgcct tccgtgttgc tgcttctatg gcattacgtg cagcagctaa gaaagctaat
     4501 ccagtaatcc ttgaaccaat catggctgta gaagttgtta tccctgaaga ttatttaggt
     4561 gatgttatgg gtcacgtaac tgctcgtcgt ggacgtgttg aaggtatgga agctcgcgct
     4621 ggcggccaac aagttgttcg tgcgatggtt ccacttgctg aaatgtttgg ttatgctaca
     4681 actcttcgtt ctgctactca aggtcgtggt acatttacaa tgacatttga ccactacgaa
     4741 gatgtaccaa aatcagtaca agaagaaatc attaagaaaa acggcggaaa agctgaataa
     4801 tttcttgaaa ccggatagtc ctgcaaagag attatccaaa gagactatat ttttaatata
     4861 gtttcgtgta aaataattat cgatgatatg ggcatgtggt gtaccttgtg tacatcatat
     4921 acctatcaga caaataaaaa atatattgta tttttaggag gacattttaa aatggcaaaa
     4981 gaaaaatttg accgttctaa accacacgtt aacattggta ctatcggaca cgttgaccat
     5041 ggtaaaacta cattaacagc tgcaatcaca actgtactat ctaagaaaaa cggcggccaa
     5101 gctatggctt acgatcaaat cgatggtgct ccagaagaac gcgaacgcgg aatcacaatc
     5161 tcaacagctc acgttgagta tgaaacagat acacgccact acgctcacgt tgactgccca
     5221 ggacacgcgg attacgttaa aaacatgatc actggtgctg ctcaaatgga cggagctatc
     5281 ttggtagttt ctgctgctga cggcccaatg cctcaaactc gtgaacacat cctattgtct
     5341 cgtcaagttg gtgttcctta catcgttgta ttcttgaaca aagtagacat ggttgatgac
     5401 gaagaattac tagaattagt tgaaatggaa gttcgtgacc tattaacaga atacgaattc
     5461 cctggtgacg atgttcctgt agttgctgga tcagctttga aagctctaga aggcgacgct
     5521 tcatacgaag aaaaaattct tgaattaatg gctgcagttg acgaatacat cccaactcca
     5581 gaacgtgaca acgacaaacc attcatgatg ccagttgaag acgtgttctc aattactgga
     5641 cgtggtactg ttgctacagg tcgtgttgaa cgtggacaag ttcgcgttgg tgacgaagtt
     5701 gaagttgttg gtattgctga agaaacttca aaaacaacag ttactggtgt tgaaatgttc
     5761 cgtaaattgt tagactacgc tgaagctgga gacaacattg gtgctttact acgtggtgtt
     5821 gcacgtgaag acatccaacg tggacaagtt ttagctaaac caggtacaat cacacctcat
     5881 acaaaattct ctgcagaagt atacgtgttg acaaaagaag aaggtggacg tcatactcca
     5941 ttcttcacta actaccgtcc acaattctac ttccgtacaa ctgacgtaac aggtgttgtt
     6001 gaattaccag aaggaactga aatggttatg cctggcgaca acgtaacaat ggaagttgaa
     6061 ttaatccacc caatcgctat cgaaaacggt actaaattct caatccgtga aggcggacgt
     6121 acagttggtg ccggtgtcgt tacagaaatc aaagcttaat tcacttaagt tttaaagtaa
     6181 agcaacattt attcggacgt aatcgctcgg aacgaaggtc ttaggatctt tgttccgagc
     6241 tttttt
//
LOCUS       DACDUU010000109         6199 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.109, whole genome shotgun sequence.
ACCESSION   DACDUU010000109 DACDUU010000000
VERSION     DACDUU010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.109"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            5..1087
                     /locus_tag="IWL30_002661"
     CDS             5..1087
                     /locus_tag="IWL30_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9707367.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1305..2447)
                     /locus_tag="IWL30_002662"
     CDS             complement(1305..2447)
                     /locus_tag="IWL30_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9707368.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2452..2637)
                     /locus_tag="IWL30_002663"
     CDS             complement(2452..2637)
                     /locus_tag="IWL30_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP9707369.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(2991..3236)
                     /locus_tag="IWL30_002664"
     CDS             complement(2991..3236)
                     /locus_tag="IWL30_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9707370.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3230..3631)
                     /locus_tag="IWL30_002665"
     CDS             complement(3230..3631)
                     /locus_tag="IWL30_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9707371.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(3818..5626)
                     /gene="uidA"
                     /locus_tag="IWL30_002666"
     CDS             complement(3818..5626)
                     /gene="uidA"
                     /locus_tag="IWL30_002666"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAP9707372.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(5649..>6199)
                     /locus_tag="IWL30_002667"
     CDS             complement(5649..>6199)
                     /locus_tag="IWL30_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAP9707373.1"
                     /translation="PEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
BASE COUNT         1996 a         1175 c          869 g         2159 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa ccggtggcaa atccatgtaa
     1141 acatggttta ttttttacac tgttttcaaa caaacccttg ctatttcttc ggttcgtcct
     1201 cgtccatttt cgataccgac agctagctta taacccaagg tatatcaaca taaaaaaata
     1261 aaaataggct atgacagcag cgtgacagca aaaaagatgg aaagttaaat atcaaacctc
     1321 gtaatttcat ttgcgagtgc ttgatttgtt tctggatata agtgtccata tttgtccaaa
     1381 gtagttttaa tagaggcatg acctagacgc ttagatattg catagatatt tttattatgt
     1441 tcaattaaaa aggcaacatg cgagtgtctt aatccatgca tatgaattgg ttttactcct
     1501 gcaatttcag ctagtttttt tattctgctt aaaatagttc ttggagatgg cggtaatcca
     1561 tcaaaagaaa aaaccaattc tatatttttc ccaattgatt gttgagtttt tttccatgtt
     1621 tttaaatctt caacggtttg tctgtctaac gaaattatcc gaatacttga tgtatttttt
     1681 gtttcagcaa attcataatc ttgtcttgta cggatgtaaa ttgacttatt tacgctacat
     1741 ctgccattta ttaagtcaaa gtctttccat tgtaacgcta agagctcctc actccttaca
     1801 cctgtaacaa aaataaaacg catcaaacgt ttataaaatt cttcataaaa atccccgtga
     1861 taaacactat tatatacttt cttaaattca tcaactgtcc aaaattctac ttgaggacgc
     1921 tgagttttta ttttacctat agtatctaca ggattttctt caataatttc aagtacaatt
     1981 gcccttttaa aaactactct caacttattg aatatctgtt taatataatt tggtgataat
     2041 ggttgctcaa tgcccttttt tacaacatgg attttcgata aaaactgttg aaattcctgt
     2101 atatgaattg gtcgaatctt tacaacctgc atttctccaa aaaaatccaa tgcacgttgt
     2161 aaaatatgat ctgttttcat atatgttttt tctacagttc ccaacttata ccaaggaaaa
     2221 aaatactctc gataaaaatc atgaaacgtt attccttgca gataaattcc ttgtgaatca
     2281 gaaaaacttt gttttaactc ttcgagtccc cttctagctt ctccttgagt tttgaagcct
     2341 cttctggtca cttgaattct ttttccaaag cgatcttgtc ctaaagttgc agaaaaaaac
     2401 caagtgcctc tttttctgtc tttataaaca ttcgcaattt tcctcatttt actattcctc
     2461 ccaagaaata tggagaactt cttctaaaat tgaaatagga attcttttta tttttgaatt
     2521 acgataaaaa aagtatcctt tctcaactaa aagttcttta cattccttga agattttatt
     2581 agcatagtac tgagaaaaac ctagattaat caaatctttt cgagtagctg ttttcattat
     2641 agcaccttcc ttactttttt tcatttattt ctatcagctg cttttgtcaa aagcacatag
     2701 gaatttcacc tctccccaag tttacggcga gccttacgga agtatcatta tcccaacttt
     2761 aaatcatcgc tttttattga ctataaaaat tattttagaa tatttatcgc tccttcttta
     2821 cagcaagaaa tcatggcgca actaaaatat gctccttaaa gtgttaacgt actgatagaa
     2881 tctatttaat tttcaatgaa caataggttt tctacctaaa agaatctact aattccttaa
     2941 ctattagaag attcttttaa acagaaaaag tggaaagggg atttttttaa ttagttaatt
     3001 ttaaatttta agatcgcttc tattaattta atttctagct gacttatcat atattcatct
     3061 acataggtaa cgaattgacc tacctcgtta aatccatttg tcaaacataa cttagccata
     3121 taagaatgat aatgtttgag tatcatcttg atcgaaattg gatctccttc tagagcagat
     3181 aagataatgg atagtggaac taataggtac tgccgctttt tagatttatc taccataggt
     3241 cataccccat tttcaattgt tcaactgcct tctttcgttg ataccagata ccacttgtgc
     3301 tcatattgag aattttaccg atttcagcat cattaaaacc tgcaaaataa taaagtagga
     3361 taatctttct tcttatctcc gataactgat caattgctgc tgataacttt tgatctgaaa
     3421 ttaatatttc cattccaaat aagaaataga catcttgttc atcaaaatga gtttgggaat
     3481 acctgccttc atttgaatct gccaatggtt cctcattatc ctttatgtta gcgtttcgtt
     3541 ttcgaataga cttagcttcg tttcgtaata cttttttaca gtaggcatca aattggtgtt
     3601 gtatagatat gtcattgtaa tgttgttgca tgataatatg ctcctttttc cccctttcca
     3661 ctaatacaac aatcagaaag cccctttttt tcgaaaaaac tagaaaaaaa acataaaaat
     3721 atttatcatt ctttaaacag aaaatctttc tataaaaaac aaaaaaaaac gattaggcta
     3781 ggctaatcgt tttttgaaca ttcttaaaaa atccaaatta ttttttataa ctgaaattgg
     3841 gtattgatga ccatcgtttc tgtagatagt aagcaatcat tttaggtttt ctatctctag
     3901 taaagattcc tttcttattt ccttgcactc ttaaaatccc ttgacttgtt gcaaaatctg
     3961 caaaattcca cacttgttca ccaacaaaat tttcacatgt atctattact tcgtgatttg
     4021 cttcgtaata agccacttga tactcttctg taaacattgt tggtactgta tcatgaaatc
     4081 cagctactgt atctgctcca tattctgtaa acattattgg cgtatcagga catctttcca
     4141 tccatccatc tagctctttt ttcaacagat attttgcatg tgccagatcc cctcccgaaa
     4201 cataccaacc ataataacga tttaaacata aaacatcgac aatatctcct accttacaaa
     4261 catctggcgt tgattttaga taagtaacga ttgttacagg tcttttctgc ggatcaagtt
     4321 ctttagttaa ttcaactaaa ggttcaaaat attctttagc accttcggaa tccgaatctg
     4381 gttcatttgc aatagaccac attacaacac ttgggtggtt cttgtcgcgt tctatcaatt
     4441 ccttgatcac cgttttatga gaattttctg ttcgtatctc tttccacgta ttatgttttt
     4501 ccacattatc atctaataaa gtggccatga aatttaaatg taaccctact gcaggtactt
     4561 catcaataac tacaatacct tctcgatctg ctaatcgcat aatttcttct gagtaaggat
     4621 aatgtgatgt tctaaaagaa ttagcaccta tccatttcat taaattaaaa tctttaatat
     4681 ttacagcctc attcaaacca cgtccattaa catacgaatc ctcatgctta ccaaatcctt
     4741 taaaataaaa aggtttatta ttaatcaaaa atttgccatt ggtaacctga acactgcgaa
     4801 taccaaattc ttctgtatag gtatcgatca ctttattatc atcaattaaa ctaattttca
     4861 tactatataa ataagcgtct agaggttccc aaaaaattgg attcttaagc tgaatcattc
     4921 ctttctttcc ttcagcttca gctacaagtt gagcctcttc gtcatatatc gctattaaga
     4981 tttttccggt ccattctgta tcgatattga attctacact tgcatccaaa tcagttattc
     5041 ttgtattaat agtaatatca cttatatagt ctttcggagt tgtatataac ttaacaggtc
     5101 tatgaatacc tgcataattg aagaaatcaa aattaactaa gttttccttt tttatatccc
     5161 cattttcctg tactgtttcc ttcataatcc ccacaggtaa agtactatcg tcaataatat
     5221 tgtttacagc aactgtaatt cgattattcc cgtcttttac aaaccgatta atttcagact
     5281 caaatggtgt aaacccacct ttgtgttgag taataaactt tccattcaaa aaaacttttg
     5341 cttcatgtgt tgcagatcca aaacgaagaa ctaatctttc ttctaataat cttttaggaa
     5401 gagtcacttc tttttcatac cacacccatc caacatgatt tctaatgttc tctgtttcaa
     5461 ataaatcatt ataagaagag ggaactgaaa tcggataact ctctttttca tttattggtt
     5521 ctaaagcaag ttcttcctgt aagtcatcat ttgtatctaa tttaaatctc cagagaccgc
     5581 ttaagtcaat tacttgcctc gtctctgtca taatgggata caacatataa accactccta
     5641 tctaaatatt aaataaatgt atcctttaaa ccacaataag catctattgg acttactccc
     5701 ttaaattcag aaagacccat tagtttgtcc accaatattt ctaaagtttc ttgttttcca
     5761 tcataagtat taatataagt ctttacttga ggtacatcag ccaaatgaaa cggacactgt
     5821 acagatacaa aaattgttgg aatttcatga atgtaaaacg gtatatccgg agttccttta
     5881 cttgctggcc agacaattcg ttgaactgtg cctggattaa cattagctat attaatgatc
     5941 aagtcatatt ttttagttaa atttttaatt ggtctcttag cagcatatat tccagctata
     6001 tttttaattt tttcttcttg cgataatcct ttcaattctt cttcaggtga aatgtatttt
     6061 tcaacttcaa atccttttgc tgacaaaatc tgtttcaata aatcaatggc ttgaggtcta
     6121 tttccagcaa ctacttcacc aaacccacct tttaccccat gtacatctac tagtaaaatc
     6181 cttttatatt tttctggta
//
LOCUS       DACDUU010000110         6119 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.110, whole genome shotgun sequence.
ACCESSION   DACDUU010000110 DACDUU010000000
VERSION     DACDUU010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6119
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.110"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            45..1052
                     /locus_tag="IWL30_002668"
     CDS             45..1052
                     /locus_tag="IWL30_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9707374.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1064..2068
                     /gene="neuB"
                     /locus_tag="IWL30_002669"
     CDS             1064..2068
                     /gene="neuB"
                     /locus_tag="IWL30_002669"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAP9707375.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2065..3213
                     /gene="neuC"
                     /locus_tag="IWL30_002670"
     CDS             2065..3213
                     /gene="neuC"
                     /locus_tag="IWL30_002670"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAP9707376.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            3186..3863
                     /locus_tag="IWL30_002671"
     CDS             3186..3863
                     /locus_tag="IWL30_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP9707377.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            3853..4995
                     /locus_tag="IWL30_002672"
     CDS             3853..4995
                     /locus_tag="IWL30_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAP9707378.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            5008..5985
                     /locus_tag="IWL30_002673"
     CDS             5008..5985
                     /locus_tag="IWL30_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9707379.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            5985..>6119
                     /locus_tag="IWL30_002674"
     CDS             5985..>6119
                     /locus_tag="IWL30_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP9707380.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     E"
BASE COUNT         2284 a          771 c         1183 g         1881 t
ORIGIN      
        1 agaatcgaat tatagatgac tataattcat aggaggagat ataagtgttt aaaggaaaaa
       61 caatattagt aactggcgga actggttcca ttggaagtga aattgttcgt cagcttttaa
      121 aatatgaacc aaaagctatc agaatttata gtagaggtga agaaaaacaa ttttatatgc
      181 aacaagagct ctcaaatcac actaatttga gatttttaat tggtgatatc agagactctc
      241 aaaggattga ttatgcatgt cgtgatgtgg atattatttt tcatgctgcc gcaatgaaac
      301 acgttccagc aagtgaatat aatcctatgg aagctgtaaa aacaaatgtg atgggaactc
      361 aaaatgtaat agatgcagcg atcaaaaata atgtaaaaca ttttgtaggt atcagtacag
      421 ataaagtagt gagtcctact aacaccatgg gtgcgacaaa gttattgagt gaaaaattaa
      481 taatagctgc caatcagtat aaaggaaaaa cagaaacaat attttcttgt gtaaggtttg
      541 gaaatgtcat ggggtcaagt ggatctgtta ttcctctttt tagtaaacaa ataaaaaaca
      601 ataaagatat tactttgaca gatgttgata tgacacgatt tatgatgtca ataccggatg
      661 ctgcaagatt agttctgtct gctgctatga attcaaaatt tggagaaaca tatgtcttaa
      721 aaatgccggc aatcaatatt ttaactttag ctcaatcatt aataaaaata tataatgata
      781 cacatgatga aaaatataga ggtgaaataa aagttgtagg tatcaggcca ggagaaaaat
      841 tatatgaaga gctaatgact acagacgaaa ttgaacgctc atatgaaaat gataaaatgt
      901 atatcattcc accacactat catgctactg acgaactgta caaagattat gaaaaaatta
      961 aaatcactgc atactcttca gatgcaacag aattaatgag tggacaggaa tttattagaa
     1021 tcgcaaaaga gaataatgtc gtgacttggt aaggagtttt atgatgaaat cagaagtata
     1081 tgtaattgca gaaattggtg taaaccataa tggaagtcta gatttagctt tagaaagtat
     1141 tgataaagct aaagaatgtg gagctaacgc ggttaaattt caaacattta aaacgaataa
     1201 gctagtttcc aaaaaggctc caatggcaaa atatcagaag gataatctta aagaggacag
     1261 ttctcaattt gaaatgctaa aaaaattaga attatctcaa tctgatttta ttagaataaa
     1321 agattattgt atacaaaata aaattgattt tttgtctact ccttttgatg aagaaagtgc
     1381 agactttcta aaacaaattg gtgtacaagg atttaaaatt ggctcaggag atttaacaaa
     1441 tttacctttt cttagaaaaa tagatatgta tgggttgcca gttttattat ctacaggtat
     1501 ggctgatatt catgaggtca gtgaggctgt agattgtttc atagatagtc cagttactat
     1561 tttacattgt acttcaaatt atccggctag ttctgaagat attaatttat tggcgattaa
     1621 cacattaaaa gaaatgttcg ggatacccgt tgggtactcc gatcattcat tgggatatga
     1681 tgttggtatt tgtgctgttg cgatgggtgc taaggtaatt gagaaacatt ttaccttgga
     1741 taaggaacta cctggtccag atcataaagc ttctttagat cctcaggaat ttgcagattt
     1801 tgtaaagcat attagaaaca ctgaaatatt tttaggtgat ggtgttaagc gagcaatgcc
     1861 ttctgaactt agtacaaaag aagtatcaag aaaaagccta gtggtcactg agaatttaaa
     1921 tgtgggagat attcttacag agaaatctct tacaataaaa cgtcctggaa atggaatagc
     1981 tcctaagttt ttatatcaat atattggcaa aaaagttaaa agaccaataa gtgcagatag
     2041 tgttctgctt gaggaagatg ttgaatgaaa aaaatactta tagtaagtgg aacacgagct
     2101 gattatggaa tatataaacc tgttgctgag aatttactac aagataattg ggatgtatca
     2161 tttgttgttt ctggaatgca tttatcccat aaatatggat ttactaaaga attgatctta
     2221 gctgacggat ttagaattag tggagaagtt caaagtttgt ttttagagaa caataaggga
     2281 aacatggctc ggtctgtggc attagagata aatggtttca caaatatatt tgaacaaact
     2341 agccctgatt ttgtattatt actaggggat agaggagaaa tgttggcagc tgcgatagct
     2401 tgtttgtatc ttggaatcaa aacagcacat attcacggag gcgaagtgtc tggtactgta
     2461 gatgaatcaa taagacatgc cataacgaaa atagcatctg ttcattttgc tgctacggaa
     2521 aaaagcaaag agcgtttaat aaaaatgggg gaagattctt ggagagtttt tcccgtcgga
     2581 gctcctagaa tagatactat tttaaataat agattaccta gttttagaga tataaaagaa
     2641 aaatatcatc taaaatttga aaagaaaaaa tacgttttgc aagtatttca tccagttgta
     2701 accgaactag aaaatataga aaaacaaatt ttagctttga tcaattcaat ggaacaacaa
     2761 tctcaaccaa ttatttgcat tttacctaat agtgatgctg gtagcgaaat tatcagaaaa
     2821 atgtaccaga aatatttaaa aacggatatt atttatatta cgaatttaga gccggaggaa
     2881 tacctgacta tattaaaaga gtgcaaattt atgatcggaa attcttcttc tgggataatt
     2941 gaagctgcat cattcaaaat accagtaata aatttaggaa ccagacaaaa tggacgagaa
     3001 caatctaaaa atattataaa tgcaaatcta aatgaaaatg aaattgaaac tgctttgaat
     3061 aaaatacaag atcctcagtt tataaaaagc ttgaaaaatg ttaagaacat ctacggtgac
     3121 ggcaacagtt ctagaagaat aacaaaaaca ttgaatcaaa tattgaatga tggggaagat
     3181 attaagtgga ttcaaaaaca aatagcttat taaaatatac tagtgatcca tacattatta
     3241 ttggggctgg cggacacgca aaagtcatta ttgaaacctt aagattagaa aataagttaa
     3301 tatatggatt ttttgatgat aagcaaaata aaatgcaaga tctgaaatat cttgggaaaa
     3361 taaacgaatt gaatagctgg gcggatagca aaaaaaaaat ctataaattt attgtagcta
     3421 ttggtgactg tgtagtaaga aaaaagatta taaaaaaatt gaatatgcca agtgagcaat
     3481 atggaatagc tgttcatcca agtgctatag tggcaaaaga aatttcaatt ggttatggaa
     3541 ctgttatttt tgcgaatgct gtaattaacc cagatgctgt aattggagag catgctataa
     3601 taaacacagg tagcattgtt gagcatgatt gtagaattaa taactatgtt catttatctc
     3661 caggtgtatg cttagcgggt ggagtacatg taggtgtagg aactcaaata ggaattggtt
     3721 ctcaatgtat ccagatgaag aaaattggat cttggtgtat gataggcgca gggagcacta
     3781 ttgttaagga tattccctca cattccttag cttatggcaa tccagctaaa attaagaaag
     3841 aaggaattaa ttttgaattc tagaatttta ttatcaagtc ctcatatggg aggaacagaa
     3901 gaaaaatatg ttgctaaggc ttttaaagat aattggatag cgccattagg tgaaaatgtg
     3961 gataaatttg aagaaagtat tacaaaatat actggttcta agggcggagc ttctgcgtta
     4021 agttcaggta cagctgccat acatcttgcc ttaagactgc ttaatgttgg aaaaaacgat
     4081 attgtctttg ctccttcatt tacttttata gctacagtaa atcctatttt atatcaaggg
     4141 gctgtaccag tgttcattga ctcagaacct aatacatgga atatgagccc tgttgcacta
     4201 gagagcgcat tagaagaagc tgaaagaaac aaccaattac ctaaggcaat catcgtagtg
     4261 aatttatatg gtcaaatggc tgattatgaa cgaattttag ctattgcaaa ccaatttaat
     4321 gttccagtga tagaagatgc agcggaatcg ttaggttcta cttataaggg aatttcgtca
     4381 ggcttatttg ggacaattgg gatctattca tttaacggta ataagatcat cacaacctca
     4441 ggtggaggag ctctagtgtc tagtgataaa aaatatgttg agcattcaag atttttagca
     4501 acacaagcaa gagatcaagc accgtattat caacatagcg aaataggtta taattaccgt
     4561 ctaagcaata tttctgccgg tatcggacgt ggacaaatgg aagtattgaa tgatagagta
     4621 gctaaacgaa gagaaatttt caatcgatat aaggaagctt tttctggaat tgatagaatt
     4681 gaaatgatgc cagaagtgga tggcatggtg ccaaatcgtt ggttgtctac tttaacgatc
     4741 aatgattata attctattga tcaagtagtt aacgtaataa ataaactaaa tgaaaaaaac
     4801 attgaaacaa gagctttgtg gaaaccacta catttgcaac cagtatttca aggggtaaaa
     4861 atgtattacc atgataataa tcttagtgtg tcagaaaagt tatttaatag cggcttatgc
     4921 ttaccttctg gaagtaatat gacagatagt gaacaagaat ttgttattga tgagtttaaa
     4981 aaaatattca agtaggtgtg atattttatg aacataggaa ttattggttt aggttccatg
     5041 gggaaaagaa gacttagact cattcaagaa cactttaatg atattttact atgtggtgtt
     5101 gatactaatg aggcgagacg aaaggaagtt gaaaaggaat ttggaatacc aacttataat
     5161 agtctaacag aggcaaaaaa tttgtttgaa ataaattcag tttttgtttg tacatcccct
     5221 ataacccatg aatttattat aaaagaggct ttaaatttag attgtaatat ctttacggaa
     5281 atcaatttat taaatgaata ttatgatgaa ataatcgaag tagctaaaaa gaaagataga
     5341 ttactttatc tatcttctac atttttgaag agaaaagaaa ttcaatatat taagtctaaa
     5401 gtgacgcata ataaaaaaat ttcttatcaa taccatgtag gacagtattt acccgactgg
     5461 catccttggg aagattatcg caattttttt gtttcaaaca aaaaaactaa tgggtgtaga
     5521 gaaatattcg ctattgaatt accttggatt atagatacat ttgggaaagt taaatcgttt
     5581 actgtaatga cccagaaatt atccaatctt gatattgatt attcggacag tttcactctt
     5641 attattgagc atgaaactgg tatagttgga actttaagtg tagatgttgt ttcaagagta
     5701 gctaaaagaa atttaactat aattggtgaa aagattttaa tagaatggga aggtaacccg
     5761 gattctctat tttcatggaa tgaagaaact tctagtatga cagatattca attgtacgat
     5821 gaggttatgc atgataataa ttattcacat actattattg aagatgcata tttagaggag
     5881 attaaagaat ttttcacgct tttggaaaag aaaatggaac cccaatatac ttttgaaaaa
     5941 gataagtata ttattaactt aattaacgac attgaggtaa attaatgatg aaagtaattt
     6001 tttttgggtt agggtcaatt ggaaagagac atctaaaaaa tctaataagc tatggaaaaa
     6061 agaattctat taattttgat ataacagctt atagtagcag tggaaaaaaa gagtttgaa
//
LOCUS       DACDUU010000111         5928 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.111, whole genome shotgun sequence.
ACCESSION   DACDUU010000111 DACDUU010000000
VERSION     DACDUU010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.111"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(170..3319)
                     /locus_tag="IWL30_002675"
     CDS             complement(170..3319)
                     /locus_tag="IWL30_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP9707381.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEE
                     QIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHA
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3333..4550)
                     /locus_tag="IWL30_002676"
     CDS             complement(3333..4550)
                     /locus_tag="IWL30_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP9707382.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(4540..>5928)
                     /locus_tag="IWL30_002677"
     CDS             complement(4540..>5928)
                     /locus_tag="IWL30_002677"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP9707383.1"
                     /translation="LLSDEESKEDLIATIVDILGYSISPEYLFNVLADQAKQATFQLN
                     DLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKKLNDVEVLGHDG
                     DVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERRFFSVFDPTMGS
                     GSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMNLRNGDTLNKDW
                     PTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADFAFLLHGFYHLK
                     ETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGTSIPTTVIVLKK
                     NRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYAHLATFDEIKEN
                     DYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAISSLQTTPENEAW
                     LQGALEVFKHEQ"
BASE COUNT         1621 a         1259 c          992 g         2056 t
ORIGIN      
        1 tgcaacagaa ccggtaaatg aatgaaaaag atggaagaat cattattcaa tagctttctt
       61 aaatactccg ttataaaatg tgaacaagta aaaagacatc tatccctatt ttctacactt
      121 ccactttact actatctttg aatcgtacaa ttttcttaac tcttctcgtt tacctcaatt
      181 cattttcaag tggaatcaac tcatcatcta acattttacg ccatgctcgc ttcatttctt
      241 gtgggtattt aaatggtttc gcttctggat gtttggcttt atatccttcg taatccttac
      301 tgttgataat tgctttcata ttaggaattg gatcttcacc tggcatatat tgcagattag
      361 aagcatgtaa ttctttctca gaaacaaacc actcattgga aaaggcatga atcaaattat
      421 cacttgcttg tgtaaaatag cgacgtttaa tgacaaagat atcttcatta ctatctaaag
      481 tccccacatc aaatgcattt aaaatttgtc ggtaaacttc tttgactcct tctgttttat
      541 tcaatttagc caaagctttt tcaatttctt ctcgtgcatc tgaactattc gcagcataag
      601 ttcctagtaa ttgatcaata taagtagaat caatatcata caacttagtg gtattttcac
      661 tataaaattc gatcgttgaa aaatcttctc cctctttttc tggttcttcc tccatcaatg
      721 aacccttcac ggtctcgtac acgccaattt gttcttccaa aagttgaatt tgattgttca
      781 aagttgaaga ttgcaccatg tcatcattgt aatcatcata agtcactaac gattcatacg
      841 cacgattcaa ttcttgaaat gctttgacgt actcgatacg gatttcaagt ggcgtatgtt
      901 catcaatgga ttccatggat gaaacctctt ttaacttacc atatgcttgc ttgaatcttt
      961 ttttggattc ttggtaactt ggataaatca aacccgattc ttctttagct tctgaataca
     1021 gttttgtcgc atcctcgaca ttcttctcca ttgtcgctgg ctttcggaaa gtaacaatta
     1081 gtccttttgt tttttcagga tacgtacggt tcgtacgtga aaaggcttgg atcaatcctg
     1141 catactctag attacgatcc acaaataggg tttgaatcgt aggggcgtca aaacctgtca
     1201 atagacgatc cactacgatt actaaatcga tttgtttccc aaattgtttg aattccgctc
     1261 tttttcgtgc caaacgatta ttgatgtctc cgttataacg ctcaatgtct gctagtgacc
     1321 aggcagtatc ataatactga ttatattcct caataatttt ttgcatttct tcttgttggg
     1381 cacttgaatc tcggctattt tcttctaatg aataggtaat agcgattcgt gggaaatcag
     1441 gatcctccat cgttcgtcct tcacggatag gctgattggg aaactctttt attaaccaat
     1501 cagattcttt cgtcatctct ttgatggccc gataatactt tttggccata tcaatggaac
     1561 tcgtggttaa gatcgcagac ttcgttggac ggccatttcg aaaatcaaat tttgtatagg
     1621 cattgtctgg acggaacatt ttatgaatga ctttttggat atgttcctct gcttcaaaaa
     1681 cagtagggtc caaataactt tcttttttga cagactccat ttgatcgatc atacgattga
     1741 tctgttccgg tgaatactct gcatatgttt ctacttcttt taatttccga tagattttat
     1801 tctctaatga agtcggctcc acggtgtttt catgctctac ttgaaatcct aaaaccgagc
     1861 catcttctaa tgcattcttg atggtatagg tgtgcaagac ctctccatac tgatcatggg
     1921 tcgtacgtgc caattgccct ttggcttgtt tccgattctc agggaaaatc ggtgtacctg
     1981 taaagccaaa ccaagtggat ttcggaaaca tttttttgat ttcttccata ttttcagcac
     2041 ttaacgctcg atgacactca tcaactacaa acacgatatg ttgccccatt aatttttgga
     2101 agcgattggt tccttttttc tcttcctgtt tctttgcata tttcaacgcc gcatctagtt
     2161 tttgacgggt tgtaatgatc acaacattcg cattggcatc tgccaacaga gtttcactta
     2221 actctttggc acttcctgtt cccacgatca agctattcgc tttggcatta ccagaagaaa
     2281 ccccagtatt aaactccgaa gcaaatttcg taaactctgt ggtggtctga ttatctaaat
     2341 ctttgcggtc cactaacatg atcgttcgat cgatccccga ttttcgtgct aaaagcttag
     2401 tagatacaaa actagtcaac gtctttcctg aaccagtcgc atgccacaca taccctgatt
     2461 gatgtttatt tgcggcaata aacaaggctt gaatcgcatg cacttgataa ggatgtaaca
     2521 ccatgagggt tttattgtct tgatcttcac tgacaatcgt ataatccgca atcaagcgat
     2581 gggcatctgg gatatttagc acttgtttgc aaaactcata taagttttct acctttttat
     2641 tatctttcgt tcgccaacta aataaaaact taggatgcaa atccttcggt aaggcatttg
     2701 caaaataccg agtcgtttgt tcattggaaa ccacaaacaa ctgtaaggtc gaaaagatat
     2761 tgttccgaaa catgccttct tcagcatatt ttttgatttg attaaacgcc tgataaaaac
     2821 catcttttgc agttacttgt tttagctcga tttggataat cggtaagcca ttgatcaata
     2881 aggtgacgtc aaaacgtcga tcccgcgctt ctatgttact tccacgtttt gcaatttgat
     2941 ggaccacttc ataagtagag attcctccac caatgtcttg attggaataa aggataagtg
     3001 agacagaacc caattgcgga ttttcccgtt caatcgtgat ccgagccatc ccattttcac
     3061 cttttaacca tttcgcagca tcaaatgggg ttttcgtccg tagcaataat tcggttttga
     3121 tcgtttcaaa ctctttatcg gtcaaaggag aatcattcag ttctgcttga ttatttgaga
     3181 tgattttttg acgtaaattg ctccaaagat cttcttctga ttttagatcc ggacgatacg
     3241 tccactgatt atgtccttcc ccgaggacac gaatcaactg ttcttcgact tccgcttcct
     3301 cacggtgact aattttcatt cttcttccct ccctaaacaa acatcttttg caagaagcct
     3361 tttttcgttt ccttcagcaa atctaactta cgttgatgaa gagcgatcgt atcatctagc
     3421 tgcttgaaga aggcaccaat tttttgttgt tcatttatat ccggtggaaa ctgaaatgtc
     3481 ctttgttcta taccaccttt agtcacatgt accattgtcg ttccgttagt tgattcttta
     3541 attttctctt tatcagtcaa tagccaagta tatagatact gtttgtccaa ttccttagat
     3601 aaaaatttaa gtttccaaat atgataatgg tagattacac gttcttcatt ccacatttca
     3661 ggaccgaagc ttgttgccca taaatataag agatctcctt tattagcata cttgtcttcc
     3721 tctaattcta agtttgaata ataccatcta tcattagtat taaaattacc tactcttaaa
     3781 accctatatt ttccagaatc aagtaattct tgttgtttaa aagctctacc attcaaaaaa
     3841 actaccactt cccccaactt ccgctcttcc caatctcccg taaatcctgg aaaacgaact
     3901 tctggcactt ttgctccgtt tttagggaac attttttgta agaatccttt tttcgtttcc
     3961 ttcagcaaat ctaattcgtg ctggtgaagc atgatggtgt gatcaagttg tttgaaaaat
     4021 gaaccgatct tgatctgttc gtcataagtt ggaagtgaga taggtgtctt tttaccacta
     4081 tcaattgtat aggttccgac ggttccaatg cctgcaacgt cacggatata cttctgaatt
     4141 ttgttattta tgaacgagta gtaaaagaag tttccgtcaa ttttatgctc attcttgaac
     4201 caaatgatgt ttccatcctt aaagtaaatt gggttatcat cagtgattaa taaggggaca
     4261 cctattgatc cgacaccagt tacaagcaag tctccttgag aaactttacc tgaatttttc
     4321 gaatattcat tatatttttc ttctgaaatg taaagataat cggatggttc ctcatttttt
     4381 gcagctgaga caatatcacg agcacgtaaa aatctaatac ctgagttagt ccaatctgat
     4441 tgatgaattc gcttcacaga agtaacatcc atcatatctc ctaacttacg ctcttcccaa
     4501 tcttccgtaa aaccaggaaa ccggatttct ggtcgtgtat cattgctcat gtttgaacac
     4561 ctccagcgca ccttgtaacc acgcttcatt ttcaggtgtt gtttgcaggg aagagatagc
     4621 ttctaaaagt tctttttcta gcacttgctg ttcttgtcgt atcttcttga tgtcatttcc
     4681 gacatgaacc atatcaattg gttcttcttc ttcgaaagta tcgacatagc gtgggatatt
     4741 taagttatag tcattttctt tgatttcatc aaaggttgcc aaatgagcgt atttctcgac
     4801 atcttttctt tcagcgtagg tttcgaggat tttttgaatg ttttcttccg ataatttatt
     4861 ctgatttttc cctttaacaa attcacgact agcatcaatg aataatacat ctcgagtttg
     4921 acgattcttc ttcaatacaa tcaccgtcgt tggaatcgat gtaccaaaga acaagttcgc
     4981 aggcataccg atcacagcat aaatactgcc gtcttctaat aatttttgac gaatgacccc
     5041 ttctgcagca ccacggaata aaaccccgtg tggcaagacg atagccatag tccctgtttc
     5101 cttcaaatga tagaaaccat gaagaagaaa cgcaaaatct gctttggatt ttggcgctaa
     5161 tttcccgtag cgattaaacc gagaatcatc taagaaggtc gtatctgctg accaattagc
     5221 cgaataaggt ggattcatca ctactgcatc aaatgtgtaa ggttcatctg ttggccagtc
     5281 cttattcaac gtatccccat tgcggagatt catttcttcg gcatctaccc catgtaaaat
     5341 caagttcatt tttgctagat tgtatgtcgt tgtatttagt tcttgcccat gatatttaac
     5401 gttatctgga tgagttaagt agttacgtac atttaacatc aaggaacctg aacccatggt
     5461 tgggtcaaag acactaaaga atcgtcgttc tttttgatcc aacgtcacga tttgtgccat
     5521 catatcagaa accatatgag gcgtgtaaaa ttctccggct ttcttccctg cttctgaagc
     5581 aaattgactg atcagaaatt catacgcatc cccgatcaca tccccatcgt gacctaaaac
     5641 ttccacgtca ttcaatttct tgataacttc ggtaatcgtc acatttcgtt gttgctcatc
     5701 tgttcctagt ttttttgatt ggagatccac atcatcaaat aacccattaa attgattgta
     5761 ggtagatgcc aattgcacaa aggctttgtt tagatcgttc aattggaacg ttgcttgttt
     5821 tgcttgatcc gctaagacgt tgaaaagata ctctggtgag atcgaatacc ccaaaatatc
     5881 aacgattgtg gcaatcaggt cttcttttga ttcttcatct gaaagtaa
//
LOCUS       DACDUU010000112         5900 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.112, whole genome shotgun sequence.
ACCESSION   DACDUU010000112 DACDUU010000000
VERSION     DACDUU010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.112"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(109..594)
                     /locus_tag="IWL30_002678"
     CDS             complement(109..594)
                     /locus_tag="IWL30_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707384.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(733..948)
                     /locus_tag="IWL30_002679"
     CDS             complement(733..948)
                     /locus_tag="IWL30_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAP9707385.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            1172..1972
                     /locus_tag="IWL30_002680"
     CDS             1172..1972
                     /locus_tag="IWL30_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9707386.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            1991..3859
                     /locus_tag="IWL30_002681"
     CDS             1991..3859
                     /locus_tag="IWL30_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9707387.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELCDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            3852..4343
                     /locus_tag="IWL30_002682"
     CDS             3852..4343
                     /locus_tag="IWL30_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP9707388.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            4331..4885
                     /gene="srlA"
                     /locus_tag="IWL30_002683"
     CDS             4331..4885
                     /gene="srlA"
                     /locus_tag="IWL30_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAP9707389.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            4903..5898
                     /locus_tag="IWL30_002684"
     CDS             4903..5898
                     /locus_tag="IWL30_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAP9707390.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2059 a          943 c         1191 g         1707 t
ORIGIN      
        1 aattattagg ttaatgcaaa tgcttattta tactccgtat aactttctca gaaaaaatga
       61 taccaataat gacttgctag tactgtaaat ccgtataata tcatctaatc aaaatggtgt
      121 tccatttgca tctgtcatcg taatcgatcc ttcgggtgac actcgataaa aaccaacgct
      181 tccagatcct ccttgggaac ggatttgttt gctggaagct gtgaacaaat aatctcgatc
      241 aattttttga taaaagctaa taacaatatc gtcactttgt aattcggaac ggtgggcaat
      301 aagaaataga agtgctttgt cagagttttc taatggaaga gaaggtacag tgctttcttg
      361 catttcagat gttgaagaaa tgctaaacga ctgaatactt gattggctat gttgcgaatc
      421 tacattacca gtagaagtat tagtaatgga agtagtagaa gtacttttag ttgaaccaac
      481 ctcagttgtt gaaatggctg ctgaagattc tttaacagaa ttctttgttt tatatccaca
      541 ggcagaaaaa agtattacag ttcccaacaa gcctgcgtaa atccctttat gcatgatagc
      601 tcctcctaaa atagattatc tttattcagt atatcactta aacaaagaaa agcagatggt
      661 ttaaaatgaa aaaaccttcc caataaaggg aaggttcatg aagataataa cttttttaat
      721 aattatagct ttctaatatc tttcatcaac ttttctagta atcattggga aaaatttcaa
      781 gcagtagatc gcacaaatac ctacgacgat ataaatgatc ttcgcaaaaa tagttgttga
      841 accaccagca atcatagcta ctaaatcaag ttcgaacaag cctaccaaca gccagttcaa
      901 ccctcccaca atcaataaag ttaatgcaat agcatctaaa gttttcatta gaattctcct
      961 ttcttaaatg ataaaacaat tatatatcaa aaagagaaac attgcttata taacgctcag
     1021 gaattttagc aatctatgca aaaagatgaa aatttttttt gctcttccat tagtacacat
     1081 tttttagctg aagctgtaaa tttcatgtct taattttttt tagtgagctt ttatactgga
     1141 tttgtaagaa aaaagaagga gaggatcaca tatgtcggat tggttggata ttgcaggaaa
     1201 agtagtaatt gtaactggag gttcctcagg aattggaaga agtatcgtcg aaaatttgtt
     1261 gaagcagaat gcacaagtag cgaattttga tgtaactgaa tgccgtatac agcatgaaaa
     1321 cctactatct ttaaaagtcg atgtgtcttc taaaacggac atagaagaag ggatatataa
     1381 agtaatgaaa catttcaaga cgatcgatgg tctagtcaat aatgcaggaa taaatattcc
     1441 ttctcttcta atagatagaa atcatccaaa aagcaagtat gaattatcag aacaagtttt
     1501 tgataagatg atcgctgtca atcaaaaaag tgtatatttg atgagccaag cagttggaag
     1561 aatcttggtg caaaagggat caggagtgat cgtgaatctt tcttctgaat caggattgga
     1621 aggatcagaa ggacagagct gttatgctgc tacaaaagca gcgatgaaca gttttactcg
     1681 ttcttgggca aaagagttag gaaagaggaa tgttcgtgtg gtaggtgttg caccagggat
     1741 actggaagaa accggtttga gaacacaaga atatgaagaa gctctttctt atacgagagg
     1801 tataagcgtc gaacagctga gaaacggtta cagtcgaaca agtacgatcc ctctaggaag
     1861 gagtggtaag ctgcaagaag tggcggatct agtctgctat tatctttcag aaagatcaag
     1921 ttatatcact ggagtcacga ctaatatttc aggaggtaaa acaagaggat aaaggatagg
     1981 ggggacgcac gtgacgctag taaacagatg gtaccaatta ttgaaattac tagtcactca
     2041 caagaaagtt tcaatccagg aagtgcaaaa agaaatcaaa accagtaatc aaaccataaa
     2101 aaaagatatt ggggagttga atgaagaaat tgccggtatt gcaaaaatca tccaaaaaaa
     2161 caaacatttt gaacttgaaa tttatgattt tgattttttt gatgagatca tgcaaggaaa
     2221 attgaaaaca gccagtgatt tcaattcggc atccaaaaga atcgcttatt tactaaatag
     2281 gctgatcgaa acagaggatt tcttacgtat ggatgatttg tctgaagaag tcggtgtcag
     2341 cagaggaaca gtggtcaagg atctaaagac attaaaagag atgatcaatg attttgatgt
     2401 aaagattaca ggcacaccaa ataaaggact aagaataata ggagatgagc tagaattacg
     2461 actactttta ttttattttg tttctcccta cttttcagag acgaaaacag aacagtttct
     2521 agaagaaaaa caagcaatca aacattttca gaagaagacg aatgctagta aacaagaaat
     2581 caccctttta acgaaagtta tcggtatttc tttgaataga atctctagta actatctgtt
     2641 gaagaagccc atcaatcgtt acagtgggtg ttttgaatat atccaagaat tcaatgaact
     2701 gatcaatcag ttggaagaaa tctatgaact aactttgagc caatatgaaa aagattttat
     2761 cagttttcct ttgaacatca tcaacacaag tataacgatg ataaggaaag ctaactctga
     2821 tttgcgctta tatttcgacc ccatgatgat gcgtattcga cgattagatc tcattgatct
     2881 ggatgagtcc ttcttatatc aagagatgaa acagcacctc tctttattga taaatcgggt
     2941 aatttttcgt tatcgattga ctgatttatt ttatggtgag atagaaaaaa agtatccttt
     3001 ttcttaccaa atcgctagcg aatgcataca agcattagaa gctatcttgc aacataagat
     3061 ctctaaagta gagatcagct acctaacgtt gtatttggag ttagcatttc gatatcaaaa
     3121 agagcatttt tcctctaaaa aagtagccat tgttcatacg acaggaaaag gaacagcatt
     3181 aatgatccaa agacaaatca aaaggatatt ggggagggat atacaaataa cacatgtttc
     3241 agaagaacaa tatgaaaaag tagatctaaa caaatatttc gctgttttta caacgattcc
     3301 gttaaaaaat gtaaatgaag agacaccggt gatccatttg actaatcttt ttgatgactc
     3361 ttggttaagg gcacaatggc aaaaagcaaa aaaaaagcga ttagccgggt ttcattctac
     3421 tgtttttttc tatcaaatat ggaaagaaaa aaagacgtat caagaatatt taaacaaatt
     3481 agtcgctcct ttgatcagag gctattatgt ggatgaagaa tttgaaaaaa ggatcttgaa
     3541 aagagaatat cagcaaagca catttgttaa caaagaattt gcttttcctc acacaaccaa
     3601 ccagctatct caacacattg ttttatcagt agggctaata actgatcaag gtttagcaga
     3661 aacaggagga aatggagtta tttttttatt tgccatacct gaagaaatga cggctgaagt
     3721 tgagaatgaa ttatttgagc tatgcgatat cctatttgaa ctcataaaaa tgggaataga
     3781 tgagtttatt gaattagcac cgacacaaga atcattttta aaactaatca aaagtaaagg
     3841 agagagactt catgaatgat atgtatctat tcatagtttt cgcagcttca gcttatgtgc
     3901 tgcaagcagt tatcagttgg aagcaactaa agcatttcaa tcaaacatat agcgaactgc
     3961 gaagaaaagg aaaagtagcg attggtaaac gacctgggaa aatccgttca ggcacgattg
     4021 ttatgctagc tatagacgat catgatcaag ttttagaagt cagaaaaatg caaggggtta
     4081 catctttggc aaaatttcat tcattacctg tctatgcggg tgaagatatc cattacatcg
     4141 atcgttacca tccctcagtt cgagcggaag atcgtttgac acaagcagct ttagaaaatg
     4201 ccagagaagt atacttacga atagaggcag gaaatgaaaa aagtgaacaa ccttcattgc
     4261 cttttattgc tatgctcaat aattttcgga tatggaaaag tacgatcaaa gaaaaaagga
     4321 gtgacaaacg atggatatca taacaaaatt tgctgaggga tttatgaggc tgttccaaac
     4381 aggtgcagaa acattcataa gctggatgac aggaatcgta ccagttgtct tgatgctgat
     4441 ggttgcaatg aatacgctga ttgctttttt aggtgaagac cgagtaacga aagtagccaa
     4501 attatcagcg aaaaatccgc ttactcgtta ccttgtgtta ccgttcttat ctgcttttat
     4561 gttgggaaat cctatgtcat ttacaatggc tcgtttcctt ccagaatact acaaaccaag
     4621 ctattatgct gctcaagctc aattttgtca tacgagcaat ggtgtattcc cccatattaa
     4681 tcctggagaa ttgtttgtat ggatggggat tgctcaaggg atcgaaacct tagggctaaa
     4741 ttcgatggag ctggctatcc gatacttgct tgtgggactg ctgatgaact ttattggtgg
     4801 ttggataact gattttacta ctggatttgt atgcagacag caaggaatcg ttcttagcaa
     4861 aaaagtagag ctttcagttg attagagaaa ggagagtttt aaatgtatgg tgatattaag
     4921 atcgtaagag gaaacggcgg gtatggaggt cctttgatta ttaggccgac aaaagataaa
     4981 cacaagttca tctatgtcac aggtggaggt gagaggccaa tgatcgtaga taaaatagaa
     5041 gaactgacag gtatggagtc tatcaatggg ttcaaaacat caatacctga tagtgaaatc
     5101 gcgcttgcaa tcgttgactg tggagggact cttagatgtg gaatttatcc taaaaaaggg
     5161 ataccaacca tcaatatcgt ttccacagga aaaagtggcc cattagcaca atatatcact
     5221 gaagatattt atgtttcagc agtagggtta gaacaaatac agccactaat ggaaactgat
     5281 gataacaaag aaacagtaaa tactgcatct ccagataaaa agtacaaata tgatacttct
     5341 aaaaagataa ccgagcaaaa agcagagcag caaaactgga tagcaaaaat tggaatgggc
     5401 gcgggaaaag tagttgctac attcaatcaa tcagcaagag aagcagtaga tacgatgatc
     5461 cggacagtga tcccttttat ggctttcgtt tcccttttga ttggtgtgat acaaggttct
     5521 gggatcggta atgtatttgc taacattatg gcacctttag caggaaatgc agttggatta
     5581 acaatcattg gctttatttg ttcactgcca tttttatctc ctttgcttgg tccaggggca
     5641 gttattagcc aagtgattgg aacattgatc ggtgtagaga ttgggaaagg aaatattcct
     5701 ccccaattag cattaccggc actatttgct atcaatactc aaaatgcatg tgattttatt
     5761 ccagtcggtc taggtttgga agaagcaaaa gctgagacag tagaagtagg tgtaccatca
     5821 gttctttatt caagatttct taacggcatc ccacgagtta tcattgcttg ggtagcaagt
     5881 tttggattat atagctgagt
//
LOCUS       DACDUU010000113         5862 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.113, whole genome shotgun sequence.
ACCESSION   DACDUU010000113 DACDUU010000000
VERSION     DACDUU010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.113"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(99..1229)
                     /locus_tag="IWL30_002685"
     CDS             complement(99..1229)
                     /locus_tag="IWL30_002685"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAP9707391.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1878..3080)
                     /locus_tag="IWL30_002686"
     CDS             complement(1878..3080)
                     /locus_tag="IWL30_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9707392.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="IWL30_002687"
     CDS             complement(3140..3343)
                     /locus_tag="IWL30_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP9707393.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="IWL30_002688"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="IWL30_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4880..5692
                     /locus_tag="IWL30_002689"
     CDS             4880..5692
                     /locus_tag="IWL30_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9707394.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1735 a         1209 c          938 g         1980 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa tt
//
LOCUS       DACDUU010000114         5852 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.114, whole genome shotgun sequence.
ACCESSION   DACDUU010000114 DACDUU010000000
VERSION     DACDUU010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.114"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..323
                     /locus_tag="IWL30_002690"
     CDS             <1..323
                     /locus_tag="IWL30_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptidase"
                     /protein_id="HAP9707395.1"
                     /translation="GDIITYYENSGKKISTRRVIAVENNHEDFYTKADTKTYIEPGVV
                     KKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVELKKKRRRT
                     QKFL"
     gene            451..1134
                     /locus_tag="IWL30_002691"
     CDS             451..1134
                     /locus_tag="IWL30_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAP9707396.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1443..1516
                     /locus_tag="IWL30_002692"
     tRNA            1443..1516
                     /locus_tag="IWL30_002692"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1477..1479,aa:Arg,seq:tct)
     gene            1675..2034
                     /locus_tag="IWL30_002693"
     CDS             1675..2034
                     /locus_tag="IWL30_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAP9707397.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2157..3788
                     /locus_tag="IWL30_002694"
     CDS             2157..3788
                     /locus_tag="IWL30_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAP9707398.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(3850..4491)
                     /locus_tag="IWL30_002695"
     CDS             complement(3850..4491)
                     /locus_tag="IWL30_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP9707399.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            4773..5081
                     /gene="rplU"
                     /locus_tag="IWL30_002696"
     CDS             4773..5081
                     /gene="rplU"
                     /locus_tag="IWL30_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAP9707400.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5108..5452
                     /locus_tag="IWL30_002697"
     CDS             5108..5452
                     /locus_tag="IWL30_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAP9707401.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5465..5758
                     /gene="rpmA"
                     /locus_tag="IWL30_002698"
     CDS             5465..5758
                     /gene="rpmA"
                     /locus_tag="IWL30_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAP9707402.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
BASE COUNT         1812 a          985 c         1264 g         1791 t
ORIGIN      
        1 tcggagatat tattacatat tatgaaaatt caggaaaaaa aatctcgacg cgtcgcgtaa
       61 tagccgttga aaacaaccac gaagattttt atacaaaagc tgatacaaag acttatattg
      121 agcctggagt agtaaaaaag agaaatatta tcggtagtcc catctttcag attccatata
      181 ttggactagt cctttcaaaa acggcatttg catggattca ggggttattc tttacgattg
      241 ctttttgcct aacaggaatc acagcatgga atacctttgt agagttgaaa aagaagcgga
      301 gacgaacaca gaaatttttg taagaatgtt aaaaacataa atgtattcca gagatccaac
      361 gactcttctg aacatgaaac acgagaaaac actcatgcag cttgtagatt tagtcagttg
      421 ttcgtataat gtgtgaagag gaggagtagt atgcgcaatg aaatgatgca tcgtcctgta
      481 gtcaaagaag aactattgga ttttatgcgt acccagcaaa aaaaattacc gggtgagcta
      541 ggcaaactgg aagaagaagc tcatgtagca gaagtaccag ttattcccca tgaaacagtt
      601 gtatttttga aatttttact tggacagctt cagcctgaac gaatcttgga gatcggagca
      661 gctatcggtt tttcatcaag tgtcatggcc acgacaatcg gagaaaacgc tcatgttacg
      721 acaattgatc gtttcgatgt catgatcgag aaagctaaaa aaaactatga acgtctagga
      781 ctgacagata aagtgacttt gcttgaaggt caagcagcag atatcttgcc ggagttgtct
      841 ggtccgtatg actttatttt tatggatagt gcaaaatcaa aatatattga atttttacct
      901 gaatgcttac gtttgttaag aaaaggtggc gttttgatgg tagatgacat cttccaagga
      961 ggaacgatct tgctcccaga tgaagagatt ccccgtggca aacgagctat ccatcgtaaa
     1021 ttaaatgaat ttcttcgtgt tgtgatggat catcccgatt tgacatcgac aatcttgcca
     1081 ttaggtgatg gtgtgatctt aatgacaaag gaatcagaaa caatcgagtt gtgatacatg
     1141 attatggcag cctgtagata atcgattgaa tcgagggctc aattcgaact tgatgtaaaa
     1201 gtttctatat ttgtaagtca attaaaggat acaggaaatc taaataaaca ggagtagcat
     1261 gaagtcaatg tagctcctgt tttcaattga taaccttaga acagctgaag gagaatgtct
     1321 cgttttaaaa gattgattta gtagttgggc caaaataatt cattatttgc taattaaata
     1381 actatttcgt attgacagaa gcaatttaat tatctataat tattgatgtt ccgctgattc
     1441 ttgtcatagt agctcagctg gatagagcat ccgccttcta agcggacggt cgggggttcg
     1501 aatccctcct gtgacgctaa aaaaccgtta gaaactgagg tttctaacgg tttttctttg
     1561 ttttttgtgc caatattgtt tgttttcaaa ggcaaataaa ataaaaaaac atccttatcg
     1621 agtacaatta taaggagcaa aaaaataaaa ctcatcagaa aagaggaaga agatatgacg
     1681 atactatcta gaattttagt gactctagtt gcaattgaat ttttttatat catgtatatc
     1741 gaaacggtga gaactgattc tgacacgaca agtagggttt ttaaaatgag taaagaagag
     1801 ttaagcagaa aatctgttca aacattgttt aagaaccaag gagtgtacaa tggactatta
     1861 ggagtgggac tactttatgg agcttatctt tcttccgcat ctaaagaaat cacaagtatg
     1921 ctattaatat ctattttttt tgttgcatta tatggcagtt tgactagcga tcgttggatt
     1981 ttactcaaac aaggtgggct agctctgttg gcattgcttt ctattttctt ataaatatag
     2041 aaaaaataag aatttatcta acaggaaact ttgtgaaaaa tattacttta tctcatgaaa
     2101 tatgatattc taatgaatgg tattttagaa aatttcatga aaaaggagtt agataggtgt
     2161 tcaaaaatta tgtttcctca ctaaaaaaag aattttcagg ttatgatctt tcaagatttc
     2221 agaaagatct tttggctggg atcacagtag ctgctgtagc attgccttta gccttggctt
     2281 ttggggtatc aagcggagct gatgcggccg caggtttgat tacggctatc attgccggta
     2341 tcgttattgg cggtttatca ggcggttttt atcaaatatc aggtccgaca ggtgcgatgg
     2401 cagctatctt gatgtctatt gcggcaacaa agggaatgca aggtgtattg cttgctactt
     2461 ttttagcggg tctattttta cttgcagcag gacttttccg attaggaaca ctgacttctt
     2521 ttatcccagc acccgtaatt acggggttta cttcaggtat tgctatcatt attgcattag
     2581 gtcagatcga taatttattt ggtgttcact cggaaggtac aaacgttatt gaaaaactaa
     2641 gtagttatca aacgatagga tttccgataa acacagcctc tttgatcatg ggagggctag
     2701 tcattttagg gatgtttatc tatccaaaaa aatgggcgaa acgaatgcct agttcgctgc
     2761 tggctatcat tttggcaaca gcagttatgc tattgttcca tttaccagta gcaactgtgg
     2821 gtaagattcc acaaacattg atcagtagta atcgtttgaa tatgggagat tttagctttt
     2881 ctgccttgca acaagtggca gttccagcta ttagcattgc tttattagga atgattgaaa
     2941 gcttgctatg tggtgcttcg gctggccgaa tggcaaataa gcccttagat agcaatcaag
     3001 aattaatggc gcaaggaatt ggaaatctgt tgcttccttt ctttggcggg attcctgcta
     3061 ctgcggccat cgcccgtaca agcgtggcca tcaaatctgg tgcacaaact agattagctg
     3121 gtatgataca tgcagttgtc ttactgctct ccatgcttgt ttttgcgcca gtgatgtcac
     3181 agattccaat gccagcactt gcaggtgttt tgattgtgac ggcttggcgt atgaatgaat
     3241 gggaaacgat caaggagcta tttactaata aatattggtc agcagttcta ttgtttatcc
     3301 ttacgatgat ttgtacagtt atctttgatt taagtattgc tatcgtgatc ggggtgatcg
     3361 gcggctgtgt attttttgtt gtcaaaagcg ctgctatcac gatttcatta gaaacgatcg
     3421 attggcaaag aatggggctg ccagaaacgg aaaagctgaa taactggaca gtagtgtata
     3481 ttagtggtcc actgtttttt atgtcttcag aaaaactcaa aaataaattg gaagaactaa
     3541 atcatcacga tggtgtcatt ttctccatgc gaggcgtacc aagtattgat ttgacggcac
     3601 gtgcagtttt tgatgaattt caagaaaaag cagagcaaag aggacaaacg attatctata
     3661 cttccttgca gccagaagta gaaaaacaac tttctcactt gtgggaacat acattcacgc
     3721 atcaacatca aacagttgct catgcgttga cagctttaca tgcgaaatat actgcaatta
     3781 gccattaaaa caatgaaaaa gaacagacaa gactgtaaac tgaagatttt atggtcttgt
     3841 ctgttctttt taatattgct gtaaaagtga agctagtact tgccagtctt gatggataac
     3901 aggtacaagg gagcgattgt agaaaatagc tgctggatga aaaagtggaa gaattcgata
     3961 gtttttttct gaccattcgt aggcatcctt ttgttctgtt aattgtaaga caggccctgt
     4021 atagagttga ccatgatttt tggtaaccgt atattctttg ccaagtaaac gttgaagacc
     4081 aatgtttcca agtgtcgcaa ttaattttgg ctggaccttc atcagttcgt aatccaatat
     4141 tggtgcatgc gcaaatactt cggaacgagt aggtgtccga tttggataaa cagtttctga
     4201 ctgttgtgtc cgtttattga tccgatgagt cacgcgatac gggcggctcc ttacagcact
     4261 ggtgatatac acctcttctc gtgttaagcc agttgaagaa agtgcctgca tcaattcttt
     4321 tccagcttga ccgctaaaag gaacatgcgt atcaatctct gttttccctg gggcttctcc
     4381 cacgagcatc agtttaggat gaagaggtcc ttgcccagcg ataaatcctt ctacaggacg
     4441 gccgttcatt cttaaagaag cttcttgtat cagttctttt gggtattcca tttatgctcc
     4501 tcctttttct tttattataa gaataaaaga aacacaatgc gactatttta tttggtagaa
     4561 ctgtgaggat agaaaagcag aagtattgct tgaatttttg ttttactttt tgattttaaa
     4621 tttagttgac taacggttgc tgttctggta agctagtact gttgtaattg tggactccac
     4681 agctacaacc gctcagaaca agtaataagc acagcatatg ctgtcacttc ggatggcgag
     4741 tctaagtata tgagaaggag gtgctttata gcatgtacgc aattattaaa actggtggta
     4801 aacaagtaaa agttgaagtg ggacaagcaa tctatgttga aaaacttgac gttgaagcag
     4861 gagaaaaagt tgtttttgac gaagttatct tagtaggcgg agaatctaca aaagtaggcg
     4921 ctccaactgt tgcaggtgca actgttgaag gtactgttga aaaacacggc aaacaaaaga
     4981 aagtcgttac tttcaaatac aaacctaaaa aacattcaca ccgtaaacaa ggtcatcgtc
     5041 aaccatatac aaaagtggtt atcgacgcga tcaacgctta atagcgtttg agaaagaagg
     5101 cctatatatg atcaaaggaa catttaagcg aaatgacgca ggtcagatcg tttcattcac
     5161 tttgaccggg catgctgatg ctggtcctta tggaagtgat atcgtttgtg cgggtgtttc
     5221 tgcgttagcg atcagcactg tcaacgggat tgcctcatta gccggttttg agccgattgt
     5281 tgaaatgaat gaagaagaag gcggttatct ttataccgaa gtcacttcag gaatgacaca
     5341 agaacaaaac aatattgctg agattctatt agaaaatctt ttattaggat tacaatcgat
     5401 agaagctgaa aactcagaat atatccaaat taaaacaatt aatgataaat aggaggtgca
     5461 gaccatgttg ttatctatga atctacaatt atttgcccac aaaaaaggtg gcggttctac
     5521 atctaacggc cgtgactcag aatcaaaacg tctaggtgct aaacgtgctg atggacaaac
     5581 agttacaggt ggatcaattc tttaccgtca acgcggtaca aaaatctatc caggtgtaaa
     5641 cgtgggtatc ggtggagacg acactttatt tgcaaaagtt gatggcgtag tacgtttcga
     5701 acgtaaaggc cgtaacaaaa aacaagtgtc tgtttatcca gtagctcaag aagcttaatt
     5761 gaatcttatg attaaagagg ttgtgacaag acttttgtca cagccttttt tgtttataaa
     5821 aataaaagtt gtagtggagg ctacgaagtt ta
//
LOCUS       DACDUU010000115         5793 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.115, whole genome shotgun sequence.
ACCESSION   DACDUU010000115 DACDUU010000000
VERSION     DACDUU010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.115"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            94..759
                     /locus_tag="IWL30_002699"
     CDS             94..759
                     /locus_tag="IWL30_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9707403.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(1043..2128)
                     /gene="xerS"
                     /locus_tag="IWL30_002700"
     CDS             complement(1043..2128)
                     /gene="xerS"
                     /locus_tag="IWL30_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAP9707404.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            2462..2695
                     /locus_tag="IWL30_002701"
     CDS             2462..2695
                     /locus_tag="IWL30_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HAP9707405.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            2692..3099
                     /locus_tag="IWL30_002702"
     CDS             2692..3099
                     /locus_tag="IWL30_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP9707406.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(4236..4754)
                     /locus_tag="IWL30_002703"
     CDS             complement(4236..4754)
                     /locus_tag="IWL30_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAP9707407.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1949 a          930 c          915 g         1999 t
ORIGIN      
        1 attattccta ccttattatc aagggaaagt agaagaaaga acatataaaa atcggttatc
       61 tacaatcaaa aaaacttttc ttatttcttt aaaatggagt taagagaaat acgtccaatt
      121 gacattcaaa agtggcaaaa taatttattg agcaaatata aaaattctta tgtaagaaac
      181 atgtatggga tttttcagat gtcattagat cgagcagttg ttttgggact acttgaaaaa
      241 aatcctgcaa aagttattgg aaatgtgaag aaaaggaaaa ctacagttga tttttggaca
      301 aaagaagaat ttacaaaagt tatttcaact ttttatattg atgatttgta ccaacatttt
      361 ggttttattt gtatgtggtt actttttatg accgttatgc gtttgggaga agccactgca
      421 ttaacttgga aagatattga ttttgaaaat aaaaacttgc gtgtgaataa aacactctat
      481 tataagagag ccaaaaccta tgagtttgta gaacccgaaa cggcttctag tattcgtaca
      541 atcgaactag acgacaatac aattaccttt ttaaaagctt ggaaggagcg tcaagtttca
      601 ataggaggca tgtcgtttgt actttcttat aatggaacac ctactcagaa atatacaatc
      661 cgtcatatta tgagaagaca tgcaacgatc gttggtgttc atataatccg catacacgga
      721 ttaaggcata gtcatgcttc tttgcttata tcacaataaa aagatgcact tcgaattaaa
      781 gaaaggttaa aacatgctga tgtacaaacg actttaggaa cttatggtca tttgtatcta
      841 aattcattga gagagataac aaatgatccc acagattata tccaaactaa tttttctaaa
      901 atcaaaccga cacctattta cgataataaa aaataaatga taaacaaaag taggatcaca
      961 ccaaaagaaa cacactagaa aaactataat acaggctttt ctagcgtgtt gtttcttatt
     1021 tccgcctatt tatacattat actcataaag catttaatgc atcgatcgta gccgcattgt
     1081 ctatatgggt atataaagtt gttgtattgg tacttgaatg tcccaattgt tgggcagtta
     1141 ataccaatga atttgtctgt tggtagagtc tagtagctaa tgtatgacgc agtttgtgag
     1201 ggctgacttg aattctaaag ccttttgaat attttgctac catccgttcg attgcaatac
     1261 cggatatacg cttggcttct ccttggtaaa ttgctagaaa taaagctttt tcgttttctg
     1321 ttactttata tcgttctttc cgaatagcta gataattagc taaatattcc attgcgaatg
     1381 ctgctatatt gacagcatct cttttatttc cttttctagt tactaccaca ttattcttat
     1441 ctaaagatag gtcatctaaa ttaatattta taacctcgga gagtctcaat ccagatccta
     1501 aaatcagtgc attgatcgcc aggtctcttt ctacatctcg ttgataatag atcaaagctc
     1561 gtttactcaa agtattttta tattcattgg caatataatc tagataattt tgtgtatcct
     1621 tatctagaaa taattttttc tcaatggcat tggcacgtgc agctaacgta gttttatctg
     1681 tgatggttgc tacttgatcc ataggattat tgtctaagta tggttttcct agaggattat
     1741 tggaactttt cgaaagatat ttaaatagaa cctttaatgc cgtgatattt ctattgatag
     1801 tagaagtaga tagacctaag ttattattat ctttgagctt agtttttgtt gtctcttttt
     1861 ttacattttc ttttgtgcga cgtttcaaat gagatttgta caagtttagg tcataggcag
     1921 ttaactgtct tatcgaggtg atagaagtat caggaacgat agactcactc agaagccatt
     1981 cataaaagag cttataattt tttagatatt ctaaagtggt tcgaggtgat tgatctttat
     2041 cctcacgatc ttgaacaaaa tcttttatat aatcgggcat atgaagtatg attgtatcta
     2101 ttttttgata tatttctgta tttttcatgt cattcatcct ttcaataagt aggggagaag
     2161 agtatctcta taattggact ataccaatta tagaatgttt tgtcaattgg tatgctctaa
     2221 aatttattaa tttgtttcaa aaaaagcata taaacaggta tacgtttaat aatttaaaat
     2281 aaatggtata cataaatagg gaaggaaata gaagggagca atctaaattt tttgaatttt
     2341 cttaattaat tatatagaaa gaacaatctt tctatagagt tagacatgag agggactatg
     2401 cttgatttaa aatcaacaag cgtatatact gtatatacaa agaaaaagga gggagaagac
     2461 tatggcaatt aaagaaagaa aatttaggga agtggggaac tcagtagtat taactatttc
     2521 aaaggagttt cttaaagaaa atgggttaaa tcccggagat acggtttttt tagatgaaga
     2581 aaaacttcga gcggcaatta caaaaaaaca agccttatct gatgaaattg atttagcaat
     2641 atctcaagca ttaactgaat atgatactac ttttagagag cttgtcgata aatgagtatt
     2701 cattacctag aagccaaaga tattataaaa atgaatgtaa tccaaataaa aaaatactct
     2761 cctaatgaac cccttggtgt aaaggatgcg aatgctttag aaatgtgtgt agggcaactt
     2821 cgagcctcag cttttggtga agaggtttat ccttcgattt atgaaaaagc agctatccta
     2881 cttatacaat tgataaagaa acatcctttt cacaatgcaa ataaacgtac tgctttctta
     2941 gctacttttg tctttttgaa aatcaatgga cagcttttag ctattgatca aaaagatgct
     3001 gtaaatttag cggtatatat tgctacgtat gataaggatt tcgatcaact aaaatatgaa
     3061 gtgatcaaca ctataaagac acatacgcag cccatttaag gagttactgt atgtgtcttt
     3121 atagggttat tagctgctat agcgaaataa ttgaagtaaa ttacaaatag ctataattca
     3181 aatccaacta tatagaaaac gtaaattttc tatatagttg gattaagata acgactattt
     3241 attttatcat atttttacta taccctatac ttttacttgg tatatttatg aataatcgca
     3301 aatttacccc tatactacta gtttgtaagt tagattaata aaaatactaa agttatttgt
     3361 tcttttatta aatacaccta caacttataa attgatggta tttagataac acgaaagaat
     3421 aaaaataaca gctgatagta aagtttgttg acgattgcta ctcaatcgaa agtgagttag
     3481 tgcgtatgaa cttgcttctg aaaacttatg aaatgaaaga ccttttacat cttgcagaaa
     3541 tcggtactgg gattaaacta agcaataaaa actaatgttc ccctcattct ttgaacttct
     3601 actatttgat ttatttattc tgataaatga cgatttcacc ttcaggatct tcaaatcccg
     3661 tatagaattt atttaagatc tcttcagtat cagtaatagg ataggcgata ggccacatac
     3721 cctttgatgg ttagaataaa ctgctcaaaa tctgtatcgt atgttggctt tagcatatta
     3781 acaaaggcta tctccaaatt ataaaccata agcgttgaat cgttcttaac aatttcaatt
     3841 cttcctaacc caaagtcaga agatataatt accggacttg ccttaccctt catgtgggtt
     3901 gttgagattc catatttaaa gggaattgtc ggtactatcg gcggtctgaa aaacatccca
     3961 aaaataaaaa aatctttccc ctgctagtct cctaagtccc tttgtagcgg atatggcacc
     4021 ataatctcat tttatcgaag tgagtcatgg tttatctgtt ggccaaccaa aattgataca
     4081 atctaacgat gcaccctcta cggcattcta ggggagtgca cttgcattca cccaataaaa
     4141 accggcagtt agcaaagaac ttatgtagtc gtatgttctt actgttaaca tacaagaggt
     4201 ctcctttcca caattgctta gcagtaaacg ggaatttaca aagctaatct cttccatttt
     4261 cttatctttg ttctaaaagt cccaaatgga gcaactgtat ttacatgaat aaatttatat
     4321 acttcccata cagccgtttt ggttgcctcg tctgcccatt ttcttttgtg tgcagtgaat
     4381 agatcttcat cactcattga atcaatcatc atgttaatat catcaatatt acgattgaga
     4441 atttcctcta attctttcaa tggtagatgt gcataggtat ttgtaaacca cttatataac
     4501 tctcctaact gattccattt aaattgatca gaaggcgttt taacagataa tccatttctt
     4561 tcatcatttt cccattttag aattaaattc gtccaaccaa cttgatatgc taaattttca
     4621 gcaggtgttc tatccacttc ctcaactctt ttatccttca attcttcagg aatagtttca
     4681 aattctaaaa tatatttagt ataagtccta tttatttctt tttttaattc ttctttattt
     4741 ttatatgttc tcattatatt acctccataa attcctaatt gtctccttta attcatctat
     4801 aatttcatcc acgatatttt aatccgttct acaatgcata tctgatatgc attgactgtc
     4861 taactcatat gtgaatgatt tataaaatat cccaacgtct catctaaaga aatgttgatc
     4921 gtcagcaagg agctaaaaaa tacgaatata tttcttttgt agtaaaatcg gactggtttg
     4981 catatcattt tcttcgtaaa tatcaaaatc atgttaacta ctatcaatta ctaataaaaa
     5041 ctaccacttt aagcgttaag aactgttaag tttagttaag tgaggatagt tcacaaaatc
     5101 cattgttaaa cttcacgaaa gtggtaaaac tcaaaatgct ttatatagga ttacagcatc
     5161 gctctttctt cgattgcgcg ttgggtaaaa aatattatga agtaaaaatt gacgatgaca
     5221 cgttccttac ggctcatcag atgaaacagc tacaaaaacg aaatgatcaa ctcgaagaaa
     5281 atttaattct aaaaaagcga ttgccatatt cacgcctcgc tcgacgaact attaaaggtc
     5341 aagatttacc tgaagatcat cctttttagg aaattgaagc tttttatgtt gcacaatgca
     5401 tttataatac tacaatgatg ttttcaccag atattcttct agggtgcggt gtaatgaaac
     5461 agatacactt gcaaaagaaa attcaccgcg aatttactaa aattatgaat aattatgtta
     5521 atcttccgaa atttttttcc aaaattaagg gatcaagctg gtgttataga ttgtttaagg
     5581 ttggcaaaaa aataacttct ttttaaacaa ttatagaaaa ccgatactct gttaagttgg
     5641 ttgacacggt gtcgattttt atgactttct tattctgaaa cttcactaac ggacagtacc
     5701 taaaaagtct atagtatgga cgtttacgag tactatttta tcaaaaatgc tgctcatttc
     5761 aacttatatg ttttagtgaa ctacccccac tta
//
LOCUS       DACDUU010000116         5686 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.116, whole genome shotgun sequence.
ACCESSION   DACDUU010000116 DACDUU010000000
VERSION     DACDUU010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.116"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(485..1294)
                     /locus_tag="IWL30_002704"
     CDS             complement(485..1294)
                     /locus_tag="IWL30_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9707408.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1294..2040)
                     /locus_tag="IWL30_002705"
     CDS             complement(1294..2040)
                     /locus_tag="IWL30_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9707409.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2058..2525)
                     /locus_tag="IWL30_002706"
     CDS             complement(2058..2525)
                     /locus_tag="IWL30_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9707410.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2525..2935)
                     /locus_tag="IWL30_002707"
     CDS             complement(2525..2935)
                     /locus_tag="IWL30_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP9707411.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2946..3959)
                     /locus_tag="IWL30_002708"
     CDS             complement(2946..3959)
                     /locus_tag="IWL30_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009141002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9707412.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4176..4910
                     /locus_tag="IWL30_002709"
     CDS             4176..4910
                     /locus_tag="IWL30_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9707413.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVSNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4894..5604
                     /locus_tag="IWL30_002710"
     CDS             4894..5604
                     /locus_tag="IWL30_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9707414.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQSGSVAKF"
BASE COUNT         1830 a         1195 c          942 g         1719 t
ORIGIN      
        1 cgacaaaaac cacgagcagg tccattcatc ctcaaacctc ggtgatcttc gcggtttaac
       61 accatcagaa gaaatgcaca aaaataataa ggagagtctg agacataaat aaattcccag
      121 acttttagtg agtctaaatc cgaataaacg gtgggtcgaa agcaactcta gctgcgcgtc
      181 gatcgcagca atctctaggt gctatgttcg gataaatggt ggtcaaaagt cagattttcg
      241 gccatttcgt aacagccaac cagacaaaaa gcgtctgttt tgtcttctac tccaattgct
      301 caaatcttag cgacttttgg cttggtctta ctctaaacag tttgtaagcc taaactttat
      361 ttgctgtttg tttctaagat caaagcaaaa ctacttttta gttggttact tttacaagtt
      421 ctttttactg ttgaataaat gtaaatctta agaacaaata tttacttcta taattccaag
      481 tttcttaaaa aattccgcca agtgtaccta aaaagcataa aacaaaaatt cctagaataa
      541 ttgtgatcgt tttatgccct ttggagtaaa gccagacgca taaaaaggta attcctaatg
      601 gcagcaagcc cggaaaaatt tgatcaaaaa tactttgtaa tgaagtttca gctccaccaa
      661 ccgtgaattt tagtgacgta tttaaagtaa ccatcgtgcc tatcatcgcg ccaattaccg
      721 ttaaacctaa aatcgaagca ccatgagata atttgtcaat catatggttg tcttctgtat
      781 catcaataaa tttagttcct aaaacatagc catattttgt cgccaaccat ttaaaaataa
      841 aatgaatggc agtatacaaa gtaaaataaa ggatcatgcc taaaatattc ccttgttgag
      901 caaaagaaat acctatgcca gccgcaataa cccgaaaagt tccccagaaa aatgaatcgc
      961 caatacctga taaaggtccc attaatcctg ttttgatttt attaattgcg tctgtatcaa
     1021 attcaggatt tttagcattt tcttcttcca tggtgacgga caagctcata acaaaaggcg
     1081 caatatgcgg tgtaatgtta aatgtttccg tatggcgctg caacgctgta taataatcat
     1141 catcgttcgg atatatttta cgtaaggaac gttcaatacc attcatttgt ccatatgcca
     1201 tcattttatc gaaggaccaa gaggattgca taaaaaagga gcgccaaaat atacttctta
     1261 attctttttt cccaataaca ctttctttta ccattataaa tcttcctcct cttcaaaagt
     1321 tagcttttga acagccagta acgctaagca aagtccgacg acagccacac cgacaacatc
     1381 cattttcaga tacattacgc cgacaaatcc taagaaaaga taaggcatca tcgctttatt
     1441 cgccatcatt ttaattaata acgctagccc aacacaagaa agcacaccgg ccgcaacatc
     1501 aaagctgtcc aatacaacct gaggaatcaa cttgatcatc gcattgattg tagacacacc
     1561 aaaataagta gctacaaaaa ctaaaatggc cgatggaacc ccaacgatta aaggttgagc
     1621 cacataatga tatttttgtg cttttttgaa atcatgattg actaatgcct catccatttt
     1681 gttgttaaaa tacgtaattg gcattgcata ggcgaagtta cgccacatct gaagaaagat
     1741 ggacaaaggt aaagtcaacg caacggcagt tcctactcct tgacccgatt gaatcgcaat
     1801 tgctgtacct aaaacactgg aagcataaac ttcagggggc gtcgcactcc ctaccataat
     1861 tgagcccatg aacatggatt ccaaggtggc accaataata attcctgact ctaaatctcc
     1921 caaaatcaaa ccgacaaaag gcgctgtaaa tagcggtcta cccatcatcg atgcaccaaa
     1981 tgtgtattca tcaatagacg ccaaaaaagc taccagtgcg attaatattg cttgtatcat
     2041 attcttcccc tcctctttta gtcatgcaaa ggattgattt ttttgtccgt tggtacagcc
     2101 tgacattgaa ctgttatccc gttttcagtc atttttttca ccaagtccag ttcttgttca
     2161 ttcaagtaaa cggaactagt aatcattttc cgattctcgt ttttctttaa atttcctaaa
     2221 ttcacttctt taatctcagg tacgtgctga gtaatttcgt aggcatcctc caccgtcccg
     2281 acaattaaaa atacttggta tttcttgatt ttttctccat ttaaaacaga gatggcctct
     2341 gtcatatcct tcaccacaaa ttttgtccct gtaggaacag ccatttttag tgtttttttg
     2401 cgcagattat tggtagcgat ttcattactt gcaacgaaaa tacaatttgc acctaagtta
     2461 tttgtccagg caaaagcaac ttgcccatga acaaaacgtt catctactcg taataattta
     2521 atcattaaaa atcatcctct tctgttttct ctagcaactc gttcaccaaa actatccctt
     2581 cattcccgct attcatagct ttttggatta gttttttcgt gtcctgttca cctgataagc
     2641 aaagctctaa aagcagcggc agatttacac cgctgattaa ataaaaattg tctttaccga
     2701 tttgttcgga gaaagcgtta gacacgcttc ctcccagtaa atctaccgtc acaattaatt
     2761 cgtcatcagg cgctaagtcg gaaaatagct gctctatttc ctggttaatg gagtagtcac
     2821 tatctatata cgcagttatt gctaaaagtg atttttgagg accaataata aattctaaag
     2881 tatcctttaa gcctttagcc atcatggaat gcgtcgcaat tacaattttc ctcatagctc
     2941 ttaccttaat attccacagt ccgataataa cgacgaatat ctagtgaatg atcccgcacc
     3001 ttttcaaaat gagcactcac tcgttctaag gccgttgcca taaccattgg cgctacatac
     3061 tgacgcatag cgcttggtac acctgctaat tcgaaatccg cagtgtcaaa aaccgtcaca
     3121 acatcgctat atttatttac aaaacgctct acgcgatcga ctagcggacg cgtttgatct
     3181 tcacctttta ataacacaaa actcatagat ttatcagtca tttcaatagt cccatggaaa
     3241 aattcggctg catggatcga cttggtggca atccattgca tttcttccag tacgcacata
     3301 gcatattcat aggcagcccc ataagtattc ccggctccaa caaacatatg aaattccgta
     3361 tttttatgtt tttctgcaaa ttcaagtgct ttctgatcat tttgttgccg aacacttagt
     3421 aatacttccg gcattttttg taatgtttga ataaattcat catattcagg aaactcaccg
     3481 ttatttttca tcaggcgtgc acccaaagca aaaaactgta actgaagttc ttctactaat
     3541 gtatcgttgt cagcatcatt ttcaatcgca taatccacga tttccccgag tggtgtagca
     3601 gcatttccta caaacgcaat cgtcgttgca ccttgactat tggcatactt aacaacatca
     3661 acggtttctt gagtcgtacc ggttaatgaa gatgtgatta acactgtatc tttattaatc
     3721 tttttcggct gtgaacaaac cacctctgcg gctatttcat gataataatc aagctctgaa
     3781 tgagtgctta acagataagc tagcgggctg attgtggcat atgttccacc agagcctacc
     3841 aaataaagat ttttaaatcc cttctcactg attgcatctg ccacctgatt gattgtctcg
     3901 atatttttta ctgcaccatt taatactgat agaaaacgtt cttcgctaaa acctaacata
     3961 gttaaaaacc tccaagtcaa taatttgaaa aatatatttg caaatgccgg cacattaaca
     4021 aaccacttaa ccttttttat ataccacctg ttaacgtttt taatgtacca cttatagaaa
     4081 acgctgtcaa tagtttttaa ccacttttat ttttatttgt accaaaatcc ttttttcatg
     4141 tataataaac aaaaatagac tctggagtgt aacgtatgaa tactgaagaa ctaacgaaca
     4201 aattaataca agacattgaa caagaaaaat ataaatacgc gcaagctatt ccttcagaac
     4261 gtgattttgt tcaaatttat gaaatgaatc gttcagccgt aaaaaaagcc attcaacaat
     4321 tagtaaataa agagtaccta ctaaaaatac atggcaaagg aacttttgtt aaaaaaaata
     4381 tccatgaaca tttgcaaatc aaatttcatg gtttgtctga attgttggaa cgggaaggga
     4441 ttcagcctag cagcttaatt ttagcttcag aaataaaacc ggctggacac attgtcggga
     4501 aagctttaca gctggaacca actgaagaag tatttcattt ggttcgttta agaagcggcg
     4561 acggtgagcc aatttctatt gaagacactt atattccatt aaatctcata cctgatcttc
     4621 aagcttttga tttccaaatt ttttcatttt attccacatt ggctgcttat cattatcaaa
     4681 ttgaaaatat ctatcagaca atttccagct caaaagtgag caataaagaa gcccgtttgt
     4741 taaaaaccag cttaaatcgt ccggtaatca gccttgctat cactgcggtt acgacaaatg
     4801 aacaagcggt tgaatacact gaagtagtgg ttttgcctga attcgccgcc atctatacta
     4861 aaaatgttca tcaaggaaag gagatgaaaa tctatgccca aattgattaa tctgttggct
     4921 tatgacctga ttgacaaaat tattgcttat attcatgaac atcatttaac ccccggaaca
     4981 aaactgcctt cggaacggga cctatcagaa atgtttaatg ttaatcgaac taccttaaga
     5041 ggcgcgttac aaaaactggt ggacaacggc accttacggg cgcaaaccaa tagcggctat
     5101 tatttaactg cgccaaaatt cgaaaaaaat gcccatcatt tctttacacc gatagaagat
     5161 tctctgctta gtaagcagcg gctacagcta aaggcaacgg ctctttcacc gaaaaaagca
     5221 gcagctcttt gtccggaact gaatgatttc aaacagcagc aaccacaagc ttatttagaa
     5281 tatattgatg cagaagcaat tagcttgatg ttattaatcc ctatggaaac attaaagaaa
     5341 agtaatttct cggacaatcc tttccaagat tttaaaagtt tggcatatac gcgttctcaa
     5401 gaaatttctt taataactga caatgacgtt gcggcggact tgctgggagt tgacaaagaa
     5461 gaggttctgt tatttgtacg agaggaaata agagccggta aggagttggc ggcgattgct
     5521 ttaggttata ctattagtcg ccgctgcaac atacaggtta atgttgctac gaccttgcaa
     5581 tccggttctg ttgcaaagtt ttaaatctac tatcagataa ggtagaataa tagaaaaaga
     5641 tagcaggagg aatgacgatg agtcatttta aaggaaagca atttca
//
LOCUS       DACDUU010000117         5643 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.117, whole genome shotgun sequence.
ACCESSION   DACDUU010000117 DACDUU010000000
VERSION     DACDUU010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.117"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..138)
                     /locus_tag="IWL30_002711"
     CDS             complement(<1..138)
                     /locus_tag="IWL30_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP9707415.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LK"
     gene            complement(232..1167)
                     /locus_tag="IWL30_002712"
     CDS             complement(232..1167)
                     /locus_tag="IWL30_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707416.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
     gene            complement(1726..2043)
                     /locus_tag="IWL30_002713"
     CDS             complement(1726..2043)
                     /locus_tag="IWL30_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP9707417.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            complement(2044..2298)
                     /locus_tag="IWL30_002714"
     CDS             complement(2044..2298)
                     /locus_tag="IWL30_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9707418.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            2657..3061
                     /locus_tag="IWL30_002715"
     CDS             2657..3061
                     /locus_tag="IWL30_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAP9707419.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
     gene            3078..4226
                     /gene="tnpB"
                     /locus_tag="IWL30_002716"
     CDS             3078..4226
                     /gene="tnpB"
                     /locus_tag="IWL30_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP9707420.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTIALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(4449..4667)
                     /locus_tag="IWL30_002717"
                     /pseudo
     CDS             complement(4449..4667)
                     /locus_tag="IWL30_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(4933..5127)
                     /locus_tag="IWL30_002718"
     CDS             complement(4933..5127)
                     /locus_tag="IWL30_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707421.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
BASE COUNT         1904 a          979 c          855 g         1905 t
ORIGIN      
        1 cttcagttga tcagtatttc ttcggttttt aatcttatct ggtacacaat tgtggtatat
       61 gttagaaaca gttctcataa tttccttgaa tgttataaaa gtatctgtat aatgttcagt
      121 atattttgat gaacgcatat gtagtcctca tttcttggga gttttgagac tactctatgc
      181 gtttttttgt tgatttacca acatgtttag acatttttct ctaggaaaaa attaactagc
      241 accacgggtt cctttatttt tattaacaag ttttatcaca ggcttcatat atttgattat
      301 atcttcatct tctacataga tatctgttat cattacgaca tctgtactat ttcgattgag
      361 tactttcact tgatctccag cattgatttt ccctctaaat cttttaggaa tgaaccaact
      421 atacatatga ttagcatcac caaaatgtgc ttttatcgca ttctcaggat ataacttatt
      481 caatagttca ttcttttgaa ttttcaaaac ttcaaatcta tacttatcat cttctgctaa
      541 tctaaataaa tcttctaaac gcatgttttc taactgtaag ttttgaaaat cttccggtat
      601 aaattttcta tatttcatta ataatgaacg cccagcccga tttattagaa tctggccatt
      661 ttggtcatct ctataaagaa ttccgtaacc catcaacgcc aatagcacac gtgacgtttt
      721 gttacttgta atattcatat ttccttgatt acctgaggca atgaaagact tatctacttg
      781 aattagggtg aaattctcat tcaaatatgg tagtaaaatt aatatatgtg aaaatcgaat
      841 cttgagatag ctattcccct tgagatattc actgatactt ctactcaaat catttaaatt
      901 tttataatta tttgtacgga acgtatatat atcaacatca gttttcttta catgagaact
      961 tattctaaaa tgaaaaagcc tactctcaga aagaattgaa aaatagatac tttcagtaga
     1021 tactgaagat tgaacaaaat taaacttgta tccttcaggc atagcacttt taacagtctg
     1081 ctgcattaac tcggataatc ttttgaaagt agtaatatat tttttaccag caaaccatac
     1141 atatcttttt tcattaagta tgttcactat ctctcctcca ttaattatat ttagcatcaa
     1201 atagttattg tacctatatt ttatcccatt ccttttaaaa caagaatacg tacaaagtaa
     1261 aatgttgtaa atagtctagc agggatgtat attttcatgt accaacataa tatttaagaa
     1321 gtttcccata gatatattgg acttcctctt ttaaaacaca aaaataaata ctaccttatt
     1381 taggtagtat ttattaaaag ttacgtcaat tttgattaaa aatatactac ttgaacctct
     1441 catgagtaaa gtcacgaaat ttttgggaat agagcgtact tgatatctta ttttttcact
     1501 aatagcagtg tctcttattc accaagcgat ccccgtagtt cctacggttc ttaatttttc
     1561 tctagactaa tgattgtatc tttagacctt cggccataac atggagcaaa ataaaaaggt
     1621 actaagtctt atatatcaat cttcgagtaa ccgacggtta acaaccatat cgagcggcat
     1681 taatcaccca cctgtagagg atgagcgact ttttcctaat tattgttaaa agcctagttt
     1741 catttgttta gattggaaaa tacgtcctac aagtatctct tttttttcat ggtcgatccg
     1801 ataaaataag gcatagcttt tgccgatcac gatccgatat gttgggatat ccattccata
     1861 gatttcggaa acttcctcat gcatagttgg aaaatttttg gttagttcta aagccttctg
     1921 gatcgcacct acaaatcttt ttatgctggc tggagaaaga gcaagctcat tttcccattc
     1981 ttctattatc gtttcaaggc tggtctgaaa acttttagaa taaactagcg tgtactcttt
     2041 catctattca ctccagccgt attttccgaa agcttttacc atttcaattt ctgaaactgt
     2101 taaacctttc tcaaagtctt ggaaactttc ttcaatcaat ttgcgatcgt gttcttttgc
     2161 tatttgagta gtatctaata tgaataagtt gccttcttgt ttggcttgga taatgtctcc
     2221 gctttttaat ccaagagagc ttggaaggat cactcccacg ctattgccga tctttctaac
     2281 ctttagttct tttcccatgt tatcacactc cttttagttt ttgatgaata aattataaca
     2341 tgttataaca acaattgcca acggtgtttc ttaagggatt ccactccata ttattattgg
     2401 acaaaataag ccacttagaa cgctgaaaac atatacaaac gatatcaaaa tacgagtgaa
     2461 ctactcacca tacacttccg ttaacatcca tttaacagaa ttaggcagaa gtcgcccatc
     2521 ctctataggt gggtgatgaa tgccgttcgg tatgagggtt accgttggta actcgaagac
     2581 cgacaactaa ggcgtattgc ctttttattt tgtcccatgt tacaatcagt ctatacaaga
     2641 aatgagttga tataacatgg aattagatac aaataatcat tcagtatttc ttctttatta
     2701 ccaccttgtt ttagtaacga aatatcgtag acaagtgatt gatgatgaaa tatctgatta
     2761 tgccaaaact acttttgaaa gaatttcaga atcgtatcac attactttag tggagtggaa
     2821 tcatgataaa gaccacgttc atattatgtt caaagctcaa cctaaaacag aattgacaaa
     2881 attcatcaat gcctataaaa gtgccagctc aagattgata aaacgtgact ttccaagagt
     2941 caaacaattt ctttggaaag aaatgttctg gtctaaaagc ttttgtcttc tgacaactgg
     3001 tggcgcacca attgatgtga ttaagaagta tatccaaaat caaggaaaca atcataaata
     3061 gaaagtaggt gaactttatg gaacaactaa aagcatacaa attcagaatc tatccaacag
     3121 aggaacaaga aatcttcttt gccaaatctt ttggttgtgt ccgtaaggtc tacaatctaa
     3181 tgcttgatga ccgaaaaaag gcgtatgaag aagttaaaaa tgattcttct aaaaaaatga
     3241 cttttccgac accagctaaa tacaagaaag aatttccgtt tttgaaagaa attgatagcc
     3301 ttgctttagc caatgcccaa cttaatttag ataaagcata caagaatttt tttcgggata
     3361 aatccgttgg atttccacgt ttcaaaagta agaaaaatcc tgttcagagt tatacaacga
     3421 ataatcaaaa tggtacaatt gctttgattg atagtaaatt catcaaagtt cctaaattga
     3481 agtcattagt aagaattaag cttcatagac aaccaaaagg gattatcaaa tctgctacaa
     3541 tatcacgtca ttctagcggt aaatactata tttctttgtt gtgtaaagaa gaaattatcg
     3601 aattacctaa aactaattcc acaattggta ttgacttagg tattatggac tttgcgattc
     3661 tttctgatgg acaaaaaatt gataataata aattcacgtc caaaatggaa aagaaactaa
     3721 agcgtgaaca acgcaaattg tctaggcgtg ctttattggc taaaaataaa ggtaccaatc
     3781 tatttgaagc taaaaattat caaaaacaaa aacgcaaagt agctagattg catgaaaaag
     3841 taatgaatca acgtactgat tttctgaata agttgagtac agaaatgatc aaaaatcacg
     3901 atattatctg tattgaagac ttaaacacaa aaggtatgtt gcgaaatcat aaattagcta
     3961 aaagtatttc tgatgtttct tggtcaaagt ttgtgactaa attacaatac aaggctgact
     4021 ggtatggacg aaaaatcatc aaagtagata aatggtttcc gtctagtcag atttgttcag
     4081 agtgcggaca taaagatggc aagaaatctc ttgaaattcg agaatggact tgtcctgttt
     4141 gtcatactca tcatgatcgt gatatcaatg ctagtatcaa tattctgacc gaaggtctaa
     4201 gactacattc tatgggattg gcttagatcc aatcatcaag aaccgtagga actacgggga
     4261 tagcttggta aataagagac accgctgtta gtaaagaaat acgctatcaa gtatgctcta
     4321 ttcccaagaa gctcccactt caagcgttaa gaaccgtcag gtttagctaa gtggtgagta
     4381 gttcacgtag gtatttttat tatatataga tttaacatat gtcggtcggt tgattgatat
     4441 agctaaagtt ataactcaac cgaagccgat tcatctacac gaataaagtt gcgagtgttc
     4501 tcggctaatt cataaaaatt gttatgtgtt ccatatagca atactggaat aactttattt
     4561 tcttgaatct cataagcaac tctatacacg gtgaccgcat acttaaatcc attagaccaa
     4621 ttacctgata aacctccagt tttttgctct tacttaaatg gatcaatggc aatttcatca
     4681 caaacctata gaggatgagt gacttctgcc taattacgtt aaaaaatatt gaaagtaact
     4741 ctatgtagca aaaaatatct agaatttcat ttcctcaaaa ctgatcgata ttgtatctaa
     4801 aaatatatac tacctaaaat aggtagtata tattaaaata tgtgcttttg ttagaaaaat
     4861 caatacataa attatcatat ctgtatatgt catttaggta tttcagttat gttataatag
     4921 ttttgctgtc tgctaccccg gcctaatctg gcaaggggag gaggagatat aatgaaatac
     4981 ctttttgatt ttgtttttgc aatcaagcca cttgcagatg tagtgtgcag ttacctctgc
     5041 aaatggcttg attgcaaaat tgccaaaaaa gacagtaagc ctacccgtta attctataat
     5101 atagaaacgt aaaaaaaacc ccatcagcct gcacgctgat ggggtttggt gatataatga
     5161 aatacctctt tgttactcat aattatacca taattagtca cgaatgcaac caatagaaaa
     5221 aactacttag aacggtagat catcgctaat aacaatgcat tgtccaccaa atggattttg
     5281 tgaagcatta aatacattgt tattaattgt cgctgctttt aactaggcct ctccccttct
     5341 attataaaag aagatatata tcatttctta attacctact accttaaacc aaaatttaag
     5401 tactacctaa tttaagtagt acttaaacaa tctaaaaaat agttatccca attgttatgc
     5461 ttttataata cgattatttg taacaaaatg aatattatat aattaaacac taccatattt
     5521 aggtagtgtt taattattag caaaacaaaa aatatttatt ctattctctt aacttcaact
     5581 aagttatgct gcacaccttt atataaatca tatgcaactc attagctaga caaactacaa
     5641 gca
//
LOCUS       DACDUU010000118         5537 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.118, whole genome shotgun sequence.
ACCESSION   DACDUU010000118 DACDUU010000000
VERSION     DACDUU010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.118"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..2155
                     /locus_tag="IWL30_002719"
     CDS             <1..2155
                     /locus_tag="IWL30_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP9707422.1"
                     /translation="TTEFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADAN
                     ANVVIITTRQKLDAALKYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEI
                     KKMFPKSTWFGFTGTPIFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGF
                     QVEHENTVEPTSLENKIYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEA
                     DEHIQKVIHKMFRPDNAYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDW
                     LTKEFPNQLIREGRTMEDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTS
                     WSLADIERYNGDINNRLARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNL
                     EYAGLIQAFSRTNRTYPDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSY
                     QESKKRFKQAYGKLKEVSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDD
                     MVKSPTLNNQIQLLEEQIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDS
                     TYIDQLLGTYAANSSDAREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFV
                     IKRRYFTQASDDLIHVFSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYK
                     AKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
     gene            complement(4090..4755)
                     /locus_tag="IWL30_002720"
     CDS             complement(4090..4755)
                     /locus_tag="IWL30_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9707423.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            4881..>5537
                     /locus_tag="IWL30_002721"
     CDS             4881..>5537
                     /locus_tag="IWL30_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAP9707424.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     M"
BASE COUNT         1882 a          877 c         1050 g         1728 t
ORIGIN      
        1 caccacagag tttacaaaat ttgcatcgga atttaatact gggatttctt ccgggaacgc
       61 caaagcgaat agcttgatcg ttggaacggg aagtgccaaa gaattaagtg aaaccctgtt
      121 ggcagatgcc aatgcaaatg ttgtaatcat tacaacccgt caaaaactag atgcggcgtt
      181 gaaatatgca aagaaacaag aagagaaaaa aggaaccaat cgcttccaaa aattaatggg
      241 ccaacatatc gtgtttgtag tagatgagtg tcatcgagcg ttaagtgctg aaaatatgga
      301 agaaatcaaa aaaatgtttc cgaaatccac ttggtttggc tttacaggta caccgatttt
      361 tcctgagaat cggaaacaag ccaaagggca attggcacgt acaacccatg atcagtatgg
      421 agaggtcttg catacctata ccattaagaa tgcgctagaa gatggctctg tcttaggatt
      481 tcaagtagag catgaaaaca ccgtggagcc gacttcacta gagaataaaa tctatcggaa
      541 attaaaagaa gtagaaacat atgcagagta ttcatcagaa cagatcaatc gtatgattga
      601 tcaaatggaa cctgtcaaaa aagaaagtta tttggaccca gctgtttttg aagcagatga
      661 acatatccaa aaagtcattc ataaaatgtt ccgtccagat aatgcctata caaaatttga
      721 ttttcgaaat ggcaggccaa cgaaatctgc aatcttaacc acgagttcca ttgacatggc
      781 caaaaagtat tatcgggcca tcaaagagat gacgaaagag cctgattggt taacaaaaga
      841 gtttcccaat cagctcattc gtgaaggacg aacgatggag gatcctgatt ttccacgaat
      901 cgctattacc tattcattag aagaaaatag ccgagattca agtgcccaac aagaagaaat
      961 gcaaaaaatt attgaggaat ataatcagta ttatgatact tcctggtcat tagcagacat
     1021 tgagcgttat aacggagaca tcaataatcg tttggcacga aaaagagcgg aattcaaaca
     1081 atttgggaaa caaatcgatt tagcgattgt agtggatcgt ctattaacag gttttgacgc
     1141 ccctacgatt caaaccctat ttgtggatcg taatctagag tatgcaggat tgatccaagc
     1201 tttttcacgt acgaatcgta cgtatcctga taagacaaaa ggattgattg tgacgtttcg
     1261 taagccagcg acaatggaga agaatgtcga ggatgcgaca aaactgtatt caaaagcaaa
     1321 agaagaatca ggtttgattt atccaagtta tcaggaatcc aaaaaaagat tcaaacaagc
     1381 atatggtaag ttaaaagagg tttcatccat ggaatccatt gatgaacata cgccacttga
     1441 aatccgtatc gagtacgtca aagcatttca agaattgaat cgtgcttatg aatcgttagt
     1501 gacttatgac gattacaatg atgacatggt gaaatctcca actttgaaca atcaaattca
     1561 acttttggaa gaacaaattg gcgtgtacga gaccgtgaag ggttcattaa tagaggaaga
     1621 accagaaaaa gaaggagaag atttttcaac gatcgaattt tatagtgaaa ataccactaa
     1681 gttgtatgat attgattcaa cttatattga tcaattacta ggaacttatg ctgcgaatag
     1741 ttcagatgca cgagaagaaa ttgaaaaagc tttggctaaa ttgaataaaa cagaaggagt
     1801 caaagaagtt taccgacaaa ttttaaatgc atttgatgtg gggactttag atagtaatga
     1861 agatatcttt gtcattaaac gtcgctattt tacacaagcg agtgatgatt tgattcatgt
     1921 cttttccaat gagtggtttg tttctgagaa agaattacat gcttctaatc tgcaatatct
     1981 accaggtgaa gatccaattc ctaatatgaa agcaattatc aacagtaagg attacgaagg
     2041 atataaagcc aaacatccag aagcgaagcc atttaaatac ccacaagaaa tgaagcgagc
     2101 atggcgtaaa atgttagatg atgagttgat tccacttgag aatgaattga ggtaaacgag
     2161 aagagttaag aaaattgtac gattcaaaga tggtagtcaa gtggaagtgt agaaaatagg
     2221 gatagatgtc ttttatttgt tcacatttta taacgaagta tttaagacag ctattgaata
     2281 atgtttcctc cacctttttc attcatttac cccaccccgt tgttatatga ccgtgttttg
     2341 agaggaacca ataaaaagca taggctaact aggggttcct ttttcttttg tttttttgga
     2401 atccgcttaa gtgaaatgta tctgaaatga aacggatata aagtgatttt ttgctataat
     2461 gacaaggttt tagctgtttc agataccatg taacccaaaa tagtttattg agaaaatagg
     2521 agagtatttg atactaacta gtaggaaaaa atgagataaa aattaaatgg aatcatttca
     2581 ggtaatctca aataaaaaaa tatacagtcc tgtggcactt tacctatggg actgtatatt
     2641 ttttactctc agcgggcttg ttttgactcc gtgtctgact ccgtcgtatt gattttaaat
     2701 gatattcgat gaccatttaa caggtcattt ttgaaatttt tgtctctata tgactcagtt
     2761 tgataattat tgatagatca atttgggtct atatcgaaca agggaataat ccaataaaaa
     2821 atgatttaga atatcaaaat aacaaaaaag aggactacct gtattcgtcc tcttttttga
     2881 ctagattttg accagtctaa tttacattct aatatattca tatgagctaa ttttttgatt
     2941 aaatggtaag tttttccttg tttgttttag tttaaactag tgtgtaattt aaaaatctat
     3001 atggaagggt ctgtggggta atacttaaat aaaggctagg aacctgataa catcaaggtt
     3061 cctagccttt ttataataaa atgaagcgaa aataatttct tttaaagagc cgagattatc
     3121 agctatataa gagtgaattt gatgcaaaaa acagagccga aacagtcttg gtgttcgtag
     3181 ctctgcttat atgtttcgaa tttaagacaa agagttggca aaagatgttg ataaggaaga
     3241 taagataact gtgttatata aactaggtaa ctaactgtaa caagctaatt ttaagagatt
     3301 cttattagaa gacgtttagg aatataacta agaaaatgat tatacaggta attgtcattt
     3361 caataatgtt ttctaatttt cgcgtctttg ttttaagaaa aaggccagaa tcagttggaa
     3421 aaaagttcgt acgatagtga agaggggcaa aatttcctac taatattccc actaacaaga
     3481 gataaaacgg ccaggttggt gtgattcctc tataaaaaaa tgcccatgct accgaaatcc
     3541 catagattaa caactgaaca atttttacaa tgaaaaaatt gttctgccat tactcaaagt
     3601 ctcttcctcc ttgtatcgtt tatttgtttc attttatcat tatgataggc gcttgaacaa
     3661 tccattttgt attttattga gtactcttgg ataaactacc agcgattatc atatttattt
     3721 atgttactgt aagccatcat ataaaagtaa atgagcagat catagaaata taaaaaagaa
     3781 ctaaaattga tctaaattat gtgcagcttg actacgtacc aatctatatt acttactaga
     3841 ttggtcgtta atctattcgg atcgaatgag caaaatattt ttgtgaatgt ccatccttga
     3901 ttatgattcc aaaaagaatc attgggaggc tgtggcgtaa atattatact ataagccaga
     3961 aatacctatc attctgaact tttttattta ggaactttca tctcaaggct ttttcaatta
     4021 atttgtaccc ctgttttttg gataagatca tgtgcattga ttttttctat ctctacgttc
     4081 atctcctact taatgcctat ctggctcctg agggcttctt tttaaggcgt cgtcatagtc
     4141 ttggctgagg atttcttggt aatctgaggg aatggcttcc caagtgagca ggcgaacgtt
     4201 catgacgtgt tttcctttgt gtttgattac agcatggttt ttctcttttt ctacctctcc
     4261 agcgtgaaaa gacagccatg taccatggcc aaagatatca ttacataccc aacgttcgat
     4321 ggaattgtcc acgtatccta cagggagtgt ggtatagccg atttgtggtt tcaaaaaagg
     4381 attgaatcga tcgatcgttt catttttgta ttgataattg ataagaaaaa gatagaccaa
     4441 acaaaagacg agcagaaaga aagatagtgt ctttttgagt gagaagggtt gttttgatga
     4501 ataagatttc ttgttaacaa gaggttcatc tggcaatgtt ggttctattg attcggtagc
     4561 tgattcaata gaaggaaaat caatcggcag acctaaagta tgatgtaaaa gtgacagcat
     4621 ttcaaagtct ggcattgtac gagaattttc ccaactagaa atcgttttat cggaaacacc
     4681 aattttttcg cctaattctt tttgcgtaag cttacaattt ctgcggtaag ttcgtatttt
     4741 ttctccggtg ttcatcttct tctcctttcg attctacagt atatagattt attctacatg
     4801 acctatacac ttgatagata cgtttttttg atgaaaaacg gtatgatttt atcaaagaat
     4861 catttaagta agggagagtg gtgataatca aaaaaaagtt cacagtttta ctcacaatca
     4921 tagtgaccgg tatcatgagt ggttttgcct tggttagtat aaataaacag gtactctatc
     4981 ggacaaatga atttttccat caaacagata cttatactcc gattcgattg ccacaacatt
     5041 ttattgggca agaagaatat gagcagctaa tcatggtatt tgaaaatgtc tcatcagaaa
     5101 ctgagctttc ttatatgaaa aaaacagtaa atgaatccaa tgaagtagat acatcttgga
     5161 acttcaagat acagccaaag cttgaaatga cattgtatta tcaaggggat gtcaaatctt
     5221 ctcacgagaa ggtaccctat tctaatacct cctttcgcgt aacgtccatt gaagaatcga
     5281 tcaaaataaa agattttgaa ggtgaatttt tcttgatgac gagagaaaaa gaagcctatg
     5341 aacgggtcat ttcactattc actgatcgat tcaatcaagc atttcacact gattatgcca
     5401 ctaatatttt tgaagacttt gaaaatagct attcgtattc ggttatttca aatacagacg
     5461 tatataatta tcgagtgtat ttaactattg gattgattct tttattcctt ttgttatcat
     5521 cctttttctt attgatg
//
LOCUS       DACDUU010000119         5374 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.119, whole genome shotgun sequence.
ACCESSION   DACDUU010000119 DACDUU010000000
VERSION     DACDUU010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.119"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1498
                     /locus_tag="IWL30_002722"
     CDS             <1..1498
                     /locus_tag="IWL30_002722"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP9707425.1"
                     /translation="FYKYLSDRLLEQVVLLADESLEEYDTVSKQTMLYRELLSDEESK
                     EDLIATIVDILGYAIAPEYLFNVLADQAKQATFQLNDLNKAFVQLASTYNQFNGLFDD
                     VDLQSKKLGTDEQQRNVTITEVIKKLNDVDVLEHDGDVIGDAYEFLISQFASEAGKKA
                     GEFYTPHMVSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQ
                     ELNTTTYNLAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWS
                     ADTTFLDDSRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGV
                     IRQKLLEDGSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQ
                     NKLSEENIQKILETYAERKDVEKYAHLATFDEIKENDYNLNIPRYVDTFEEEEPIDMV
                     HVGNDIKKIRQEQQVLEKKLLEAISSLQTTPENEAWLQGALEVFKHEQ"
     gene            1488..2705
                     /locus_tag="IWL30_002723"
     CDS             1488..2705
                     /locus_tag="IWL30_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP9707426.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            2718..3719
                     /locus_tag="IWL30_002724"
                     /pseudo
     CDS             2718..3719
                     /locus_tag="IWL30_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(3921..5240)
                     /locus_tag="IWL30_002725"
     CDS             complement(3921..5240)
                     /locus_tag="IWL30_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HAP9707427.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
BASE COUNT         1771 a          938 c         1096 g         1569 t
ORIGIN      
        1 tttctataaa tatttatccg atcgattgtt ggaacaagtg gttcttttag cagatgaatc
       61 cttagaggaa tatgacacag taagcaaaca aacaatgttg tatcgtgaat tactttcaga
      121 tgaagaatca aaagaagatc tgattgccac aatcgttgat attttgggat atgcaattgc
      181 accagagtat cttttcaacg tcttagcgga tcaagcaaaa caagcaacgt tccaattgaa
      241 cgatctaaac aaagcctttg tgcaattggc atctacctac aatcaattta atgggttatt
      301 tgatgatgtg gatctccaat caaaaaaact aggaacagat gagcaacaac gaaatgtgac
      361 gattaccgaa gtcatcaaga aattgaatga tgtagatgtt ttagaacacg atggggacgt
      421 gatcggggat gcgtatgagt ttctgatcag tcaatttgct tcagaagcag ggaagaaagc
      481 cggagaattt tacacacctc atatggtttc tgatatgatg gcgcaaatcg taacgctcga
      541 tcaaaaggaa cgacgattct ttagtgtttt tgatccaacg atgggttcag gttcattgat
      601 gttaaacgta cgtaactact taacacaccc agataacgtt aaatatcatg ggcaagaact
      661 aaatacaacg acatacaatc tagcaaaaat gaacttgatt ttacatggtg tcgacgccga
      721 agaaatgaat ctccgcaatg gggatacgtt gaataaggac tggccaacag atgaacctta
      781 cacatttgat gcagtagtaa tgaacccacc ttattcggct aattggtcag cagatacgac
      841 tttcttagat gattctcggt ttaatcgtta tgggaaatta gcgccaaaat ccaaagcaga
      901 ttttgcgttt cttcttcatg ggttctatca tttgaaggaa acaggtacca tggccatcgt
      961 cttaccacac ggagttttat tccgtggcgc tgcagaaggg gtcatccgtc aaaaattact
     1021 agaagatggc agtatttatg ctgtgatcgg tatgcctgcg aacttgttct ttgggacatc
     1081 gattccaacg acggtgattg tgttgaagaa gaatcgtcaa actcgagatg tattattcat
     1141 tgatgctagt cgtgaatttg ttaaagggaa aaatcaaaat aaactatcgg aagaaaacat
     1201 tcaaaaaatc ctcgaaacct acgctgaaag aaaagatgtc gagaaatacg ctcatttggc
     1261 aacctttgat gaaatcaaag aaaatgacta taacttaaat atcccacgct atgtagatac
     1321 tttcgaagaa gaagaaccaa ttgatatggt tcatgtcgga aatgacatca agaagatacg
     1381 acaagaacag caagtgctag aaaaaaaact tttagaagct atctcttccc tgcaaacaac
     1441 acctgaaaat gaagcgtggt tacaaggtgc gctggaggtg ttcaaacatg agcaatgata
     1501 cacaaccaga aatccgtttc ccaggattta cagatgattg ggaacagagg aagttggggg
     1561 acttcgttgt agattatgtt gaaaagacaa gtgtacaaaa tcaatttcct atgctaactt
     1621 catctcaaca aaaagggata gttttgcaag aagactattt tgcaaatcgg caagtaacta
     1681 cagaaaataa tattggttac tttgtactac ctagaggata tttcacgttc cgtagtcgaa
     1741 gtgataatga tgtgtttgtg tttaaccgta atgacattat tgatagaggg attataagct
     1801 atttttatcc tgtatttact ttaaaatctg ctgattccga cttcttctta agacgtatta
     1861 acaatggaat ccaacgacaa ttatctattc aagcagaggg aacaggtcaa catgttctgt
     1921 ctctaaaaaa gtttaaaaat atagtagcaa tgtttcctag tgaggaagaa caacaaaaaa
     1981 tcggcacctt cttcaaacaa cttgacgaca cgatcgctct tcatcagcgt aagttagatt
     2041 tgctgaagga aacgaaaaaa ggattcttac aaaaaatgtt ccctgaaaac ggagcaaaag
     2101 tgccagaaat ccggtttcct ggttttacgg aagattggga agagcgtaag ttgggagaat
     2161 taggtaaaac tcaatcggga attggatttc ctgatgccga gcaaggtggt tctgaaggta
     2221 ctccattttt taaagtttcc gacatgaata acattggtaa tgagtatgaa atgagaaatg
     2281 caaatcatta tgtatctaat gagcaaattg agcgtaaaaa atggaagcca ataaaagatg
     2341 tgccagcgat tatatttgct aaagttggcg cagcaataat gcttaataga aaacgtttgg
     2401 tcacaagccc tttcttaata gataacaata ccatggctta tttatttaat aatacttggg
     2461 atatctattt tggtaaaata ttgtttgaaa ctataaattt gccaagatac tcccaagtag
     2521 gggcattgcc ttcttataac agttctgata ttgagaatat cagtgttaag gttcctgtaa
     2581 aggatgaaca acaaaaaatc ggtactttct tcaaacagct agatgatacg atcgctcttc
     2641 atcaacgtaa gttagattta ctaaaagaaa cgaaaaaaag cttcttacaa aagatgtttg
     2701 tctaggaggg aagaagaatg aaaattagtc accgtgagga agcagaagtc gaagaacagt
     2761 tgattcgtgt acttggagaa gggcataatc agtggacgta tcgtccggat ctaaaatcag
     2821 aagaagacct ttgggtcaat ttacgccaaa aaattatctc aaataatcaa gcagaactga
     2881 atgattttcc tttgaccgat aaagaatttg aaacgatcaa aacggaatta ctgctgcgaa
     2941 cgaaaacccc atttgatgct gcgaaatggt taaaaggtga aaatgggatg gctcggatca
     3001 cgattgaacg ggaagattcg caattgggtt ctgtctcact tatcctttat tccaatcaag
     3061 acattggtgg aggaatctct acttatgaag tggtccatca aattgcaaaa cgtggaagta
     3121 acatagaagc acgggatcga cgttttgacg tcaccttatt aatcaatgga ttaccgattg
     3181 tccaaatcga gctgaaacaa gtaactgcaa aagatggtgt ttatcaggca tttaaccaaa
     3241 tcaaaaaata tgcagaagaa ggtatgtttc ggaacaatat cttttcgacc ttacagttgt
     3301 ttgtggtttc caatgaacaa accactcggt attttgcaaa tgccttaccg aaagacttgc
     3361 accctaagtt tttatttagt tggcgaacga aagacaatga aaaagtagaa aacttatatg
     3421 agttttgtaa acaagtgtta aatatcccgg atgctcatcg tttgattgcg gattatacaa
     3481 tcgtcagtga agatcaagac aataaaaccc tcatggtgtt acatccttat caagtacatg
     3541 cgattcaagc cttgtttatt gccgcaaata aacatcaatc agggtatgtg tggcatgcga
     3601 ctggttcagg aaagacgttg actagttttg tatctactaa gcttttagca cgaaaatcgg
     3661 ggatcgatcg aacgatcatg ttagtggacc gcaaagattt agataatcaa accaccacct
     3721 gaataattca tagctttaat tctttggcac attttattga aatatgtatc actaatatcc
     3781 ctatcaactg attttgacta aaaagttact tcaaacattc actgttcaaa aatattgagg
     3841 aataaatttt gggtgtaagg cattttgagc atacttctcc ctggataact tcccagtaaa
     3901 aaaatcgtta gattgttgga ttagatccat tgactggctc gcctcaggcg agcaaagacc
     3961 ctgtagaatt cattctgata cacatgataa ctagataatt tgagatagac tcgtctaccc
     4021 gtttggacta atttcccagc aactttcaga aacttcaatc gaatagaatg aatggtcatt
     4081 cccttttgga cttcttcaaa gccaatcgtt cttaagaagt tgactaagtt gtaagctatc
     4141 aagctgagca tcattcggac atgattctcc aaaaagcgag gactgtctgt tttgtcaaag
     4201 taaaagccag ctttcgcttc tttaatgaag ttctccattg tgccacgttt ggcatagata
     4261 gaaaagatgg tatcaggaga aacattttct gatagattcg tcacgataaa ttcatgtcga
     4321 aagagtagtt cgcccgcttc acgtgttgag cgtatacaca cgcgacgact ttgtgaccag
     4381 gattgtgctt gataagtggt cgagaagtac tgaacttctc gttcttccca cttttgatta
     4441 tcgccataaa gtactgattt ctcagctatt tgacctagtc tacgattatt cttcagtcga
     4501 ataacataat gactcttttt tgattcacac gaatcataca caccaggtgt agcgaacccg
     4561 ctgtctccac gaaccaagat gtcagtgttt ggtagagagt gattatagtg ctctaataaa
     4621 ggtgtgagaa actcctttac gccttttgat gtatattgat ttcctgaacg tagttcagct
     4681 tttaagaagt caccagtcaa tccgtcaaaa gctaccaatg gatggtaacc ataggtttgg
     4741 tagtgtgtat tataattcgt ttgttcttgg tgaccaaatg tatctgaatg ggtcgaatct
     4801 aaatcaatga ttaattccgt atcattacgg atgaggcgtg ctttatcgat aagttcttgg
     4861 ttcaaagctt gaagttcatg gatattctcc tcagatagtc gatctaaaaa tcgggaaagt
     4921 gaagattgag aagcgagttc tttcctatct aaaacagctt taaacacagg atcttgtctc
     4981 aggagattag ctgcagaatc agctgagtaa ccagcaatta attgcataat gaactgctca
     5041 agtatggata agttgtcatg agtaaaatac gctcgttcgt cttcaatgtg aatgtgttgc
     5101 ttagccagtt cagaaaaacc aaagatgttc atcagctctt tcactaggac gagacccgaa
     5161 tcactcgata attgaccacc tgtatgagaa atagtgatat ttgaattgaa ttttacttgg
     5221 tttttgtgta agctagtcat gaaaagaact cctttctttt ggttggttta gtcactttaa
     5281 ccatagcaga aaggggttct tttttcatca cttaacgggt gaagatgaaa aagtaattgt
     5341 aagctgccgt gaggcagttt cacatggttt taat
//
LOCUS       DACDUU010000120         5222 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.120, whole genome shotgun sequence.
ACCESSION   DACDUU010000120 DACDUU010000000
VERSION     DACDUU010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.120"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(182..1144)
                     /locus_tag="IWL30_002726"
     CDS             complement(182..1144)
                     /locus_tag="IWL30_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9707428.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1146..2585)
                     /locus_tag="IWL30_002727"
     CDS             complement(1146..2585)
                     /locus_tag="IWL30_002727"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAP9707429.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2770..4716
                     /locus_tag="IWL30_002728"
     CDS             2770..4716
                     /locus_tag="IWL30_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAP9707430.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            4980..>5222
                     /locus_tag="IWL30_002729"
     CDS             4980..>5222
                     /locus_tag="IWL30_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9707431.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         1687 a          881 c          865 g         1789 t
ORIGIN      
        1 acagttgtgt aaaaatatca ttttagagca tgaaaaaagg agtggatttc tccactccaa
       61 aaaagtctaa cttttggggg tcactacaag ttgacaactc ctttttgttt acaaatcaat
      121 tggatatcta caaattgtat tttctaacga attttataga cattaaaaaa ttaatttaac
      181 gttaaatact tcctccagaa agtattgaga ttggtaaaat ataatcctta tttgtctttt
      241 cgcctttaat ttttttcaga agaatatcaa caataagact tgcaatttca tcaattggtt
      301 gtcttattgt tgtcaattgt gggaaaaatt tctcaataaa agaggtacca tcgtatccaa
      361 taatttttaa atcttctggg atattaagtt taagttgatg agcaattttc atagtgagaa
      421 tggctgttaa atcatcagaa acaaaaatac catctggttt gtgatttata atagttgact
      481 ttatttccat ttcacgacgg atagtagaca gattattagg aattttgaaa atttttccat
      541 ttggattttg aaaagaaaaa cctagtgcac gtaattctgt gggagaatcc gtgttatcat
      601 ttcctgtaat cataatggta ttttgacaac catttttttg taaagtctta gctgcaagtt
      661 ttcccccttc aaagttatct gaagaaacaa tggaaacatt aggagctaaa tttctatcaa
      721 aagcaacaat aggtgctctc acacgctcat aatcagcaat tccaagatta tggcttgatg
      781 agattatgcc atcaacttgg ttggcttcaa gcatttcaat gtattctctc tctttagcag
      841 gatcattttg actattgcaa ataatcgttt tatatccatg tttaaataac tctatttcga
      901 gatactcaat gagttcagag taaaagatat tactaatatt aggaaaaata agaccaataa
      961 gctgaggtga ctttccttga agtccacgtg ctaaattatt tggtttataa ccaagagttt
     1021 tcatagcttc atgaactttt tgttttgttt tttctgataa atatccctta ttattaatga
     1081 cgcgtgaaac ggttgttggg ctaactccag ctaattcagc gacatcagtt aattttgcta
     1141 ccattttaat attttaattc aaagtatttt ccagtaggtt cgcctgattt gatttgaata
     1201 ccattttggt gattttcagg gaatacacga ctagtaaaca ctttttctcc tttattaata
     1261 aaaatctcaa tgattgagtt atctacaaaa atgttagcag ttgtttcttt agtatcaatt
     1321 gagcattcac gaatcgtacc aaagtcagtg gcatattgaa tgcctgcttt gctgcgatct
     1381 aaaataattt ttccttcttt ggtatctaca atcaagctaa gtccatttcc tttactatca
     1441 gagaaaagaa gtaactccga tttctgattt ggaggaaaag ttagttcaag ctcataagta
     1501 ttatttgttt ctgcttttgc tgtaaattgt tcagaactag cacgtaatga tgtaatagct
     1561 tctactggat attggtaaag tttaccatct tttattgaaa gttcttttac aagactcatt
     1621 gctccctgat aatcatactt atcagttgga taatcgatat caggtagtcc aatccaactg
     1681 acaatcagag cacgaccatc aggagtattg aatccttgag tcgcatacgc ttcaaatcca
     1741 taatcaagat tttgaatttc tgaagcgccc acaagtttta ctttttctat gtcaagcgat
     1801 tgacagatct taaaagtatt cggatagata ttcccataat ttagctcaga tttatccaac
     1861 ccttgaggac aataaatgag aacaggtttt ttgtctacaa atactagatt tggacactca
     1921 atcatgtatt ctgaaccagt gccgccaaaa tcaaggttgc caacttcctc ccatgtttcc
     1981 acgttattgt catccgcttt gtaaagtttg ataaagcctg atttttctaa attttgtgct
     2041 ccaacaatcg catacaattg tccttcataa ttaaacattt gtggatcacg aaaatgatca
     2101 gttacatcag caggtttatg aataagaaca ttctcacctt tcgtaatgtt gtagtcttta
     2161 tccatccatg ctccgatttg aagtgtatca cgttcccaat tttcatttcg aacgtttcct
     2221 gtatagagta agaatagatt cccctctatt tcatatgcag aaccggaata agcgccgtgg
     2281 ctatctcgtt ttgtgtctgg aagaagttct gctcctgtta catgaaaatg aactaaatca
     2341 tctgattcat tgtgtaccca ttctttcaac ccatgagcag ctccaaatgg ccaattttga
     2401 taaaaaagcg tatattttcc gttaaaaaaa gaaaaaccat taggatcgtt taacaatcct
     2461 gttttaggtt cgatatgata gctagcacgc caaggtgatc gttttacatt atccttaatt
     2521 ttatccatct catctttagt ccaatcagaa taagcacgat atcgtatttc tcgaggtaag
     2581 ttcatatttt actccttcaa tttatctttt tacaactagt atggtaaacg ttttccgttg
     2641 aaaaatcaat catttcattt aaaaaaataa actatatgac aaacgtttga catattttta
     2701 agatatgcta aaataaattt gcatttcagt gaaccgtttt cacgtaaaaa taaaaaagga
     2761 gtgattcgaa tgaattacag taaagtagct tccgacgtaa tacaagcggt cggaaaagat
     2821 aaccttattg cagctgcaca ctgcgctaca cgtcttcgtc tcgtactaaa agatgacacg
     2881 aaagtgaatc aaaaagcttt agacgaaaat ccagatgtga aaagcacatt taaaattgat
     2941 ggacaatatc aagtgattat cggtgcaggt gatgttaatt tcgtttatga cgaacttatc
     3001 aaaaaaactg gactttcgga aatttcaaca gatgatttaa aacaaattgc tagtaaagat
     3061 aaaaaattca accccattat ggcattaatt aaattattat cagatatttt tgttccaatt
     3121 atacctgccc ttgttgcagg tggtttatta atggcattac aaaacttttt aacagctgct
     3181 ggactttttg gaccaaaatc aattgaagaa atgtatcctg cgattgaagg tatttcctca
     3241 atgatccaac tgatgtccgc tgcaccattc atgttcttac caattttagt aggtatttca
     3301 gctgctaaac gttttggagc aaaccaattt cttggtgctg caattggtat gatcatgaca
     3361 acaccagatc ttggtggctc aactgaatat tggaatattt ttggttatca tgtttcacaa
     3421 acaaactatg cttatcaagt tattccagtt ttagcagctg tttggatatt atctatatta
     3481 gaaaaattct ttcataaaaa attgccatca tctattgact ttacattcac gccattatta
     3541 tcagtaatga ttactggatt tttgactttc acagttattg gccctgtaat gttattgttg
     3601 tctaatggaa ttacagacat tattgtttgg ctatataaca ctactggatt tattggaatg
     3661 ggtatttttg gaggaacata ttcacttata gtcatgactg gtttacatca atcatttcca
     3721 gctattgaaa ctcaattact ttcagcgtgg acaaatggta tcggacatgg agactttatc
     3781 tttgtagttg catctatggc aaacgttgct caaggtgctg caacatttgc catttggttc
     3841 ttaactaaaa attctaagac aaaaagtctt gcttcctctg cgggtgtctc agcacttctt
     3901 ggaattacag agcctgcctt gttcggagtt aacttaaaat accgctttcc attcttttgt
     3961 gctttaattg gttcaggaat tgctacagca gtagcaggat tacttaaagt agttgctgtt
     4021 tcacttggtt cagcaggttt cttgggattt ctttcaatta atgcaacatc tattccattt
     4081 tacgtattgt gtgaattgat tagctttgta gttgcttttg caattactta cttctacggt
     4141 aaaacaaaag ctgctggtat ctttaaagca gaagaaattg ctgaacaaac accagttaat
     4201 tcttcaaaag tagcaacaac agatccaata tctactgatg ttgaatcaac acaaacaaaa
     4261 gtttcatcag aaacaattgt tagtcccatt tctggtaaag tgattagtct ttcttcagta
     4321 aatgatccag tcttttcatc agaagctatg ggaaaaggga ttgccattaa accaaatgga
     4381 aacacggtcc attctccagt agatggtact attcaactca cgtttgaaac tggacatgct
     4441 tatggtatta aatcaaataa tggtgctgaa gttctaattc atattggtat tgatactgta
     4501 tcaatgggag gaaaaggatt tacacaaaaa gttactacta atcaaaaagt tagaaaaggc
     4561 gatattcttg gagcatttga ccgttcaatg attgagaaag aaggtcttga tgatacaaca
     4621 atgattattg tgaccaatac ttcggattat tcagaagtaa atatagtagc tgaaaataca
     4681 gtcactgagg gtactgattt acttgaaatc aaataaatat atttccctga gtaaagatgg
     4741 aattttcttt ccaaaatcaa ataatataga ttaatcaaat atttgagaat aacattttat
     4801 ttaatctttt catatatcaa ttgtatgaaa aattattaga attttaaaaa atgataaaaa
     4861 ttcaaaaaga aaaaatagta gttatttact tgtcatgtta ctaaataaaa aaataattta
     4921 aatatgatat atagtcattt atttagtgat ttcattctaa aataaaggga gaaatgtcta
     4981 tgctatacgg tgcaattgaa gcaggcggca caaaatttgt gtgtgccatt ggcgatgaag
     5041 aaatgacgat caaggaaaga gtgagctttc cgacaacgac acctgaggaa acaatgcctt
     5101 tagtcattga cttcttcaaa caataccaag cagatttagc agggattggg attggctcat
     5161 ttgggcctat cgatatccat cgcgattccg ccacttatgg gtacattacc tcgaccccga
     5221 aa
//
LOCUS       DACDUU010000121         5191 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.121, whole genome shotgun sequence.
ACCESSION   DACDUU010000121 DACDUU010000000
VERSION     DACDUU010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.121"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            137..748
                     /locus_tag="IWL30_002730"
     CDS             137..748
                     /locus_tag="IWL30_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP9707432.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            821..1750
                     /locus_tag="IWL30_002731"
     CDS             821..1750
                     /locus_tag="IWL30_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP9707433.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1908..2645
                     /locus_tag="IWL30_002732"
     CDS             1908..2645
                     /locus_tag="IWL30_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HAP9707434.1"
                     /translation="MLISCGARLAPFDIRELRELMSEDELELDKIGDRKTALFVIISD
                     TDDTFNFVVSIMYSQLFNLLCDKADDVYGGRLPVHVRCLLDEFANIGLIPKFEKLIAT
                     IRSREISASIILQAQSQLKAIYKDHADTIVGNCDSTLFLGGKEKTTVKELSETLGKET
                     IDLYNTSETRSNQKSFGLNYQKTGKELMSQDEITVMDGGKCIYQLRGARPFLSDKFDI
                     TKHKNYKLLEDYDKKNLFDVEEYLKQR"
     gene            complement(2693..3871)
                     /locus_tag="IWL30_002733"
     CDS             complement(2693..3871)
                     /locus_tag="IWL30_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9707435.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKSFTEEVTSFKERELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERG
                     VKNVLLFITDGLKGMAGAVQRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKKAALEARGAFAEKWKTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE
                     "
     gene            3894..3977
                     /locus_tag="IWL30_002734"
                     /pseudo
     CDS             3894..3977
                     /locus_tag="IWL30_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            4098..4499
                     /locus_tag="IWL30_002735"
     CDS             4098..4499
                     /locus_tag="IWL30_002735"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707436.1"
                     /translation="MSDKFSKKISSDIKEDFKAIFKTASIELAGERKQLIMDKYGEDK
                     KVATACKILDEGFEDAMQVMVLPENIRQRLRTTNVLERLNEEVRRRERVVRIFPNCDS
                     VIRIIGALLMEKDDEWTSAPRKYLEFDRNEI"
     gene            complement(4706..>5191)
                     /locus_tag="IWL30_002736"
     CDS             complement(4706..>5191)
                     /locus_tag="IWL30_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9707437.1"
                     /translation="LKEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKE
                     QQVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFS
                     NNQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVI
                     K"
BASE COUNT         1641 a          879 c         1003 g         1668 t
ORIGIN      
        1 cctctaggaa aaaattaact agcaccacgg gttatgatat aataaaatgt attataggca
       61 tttttttaca agaaatagaa gcttgaagtt tatatgaagt tataaaattt aatttatatt
      121 ggaaggagga aaataaatgt attttatact acctacactt tatagccttt tatgtgtaat
      181 ggcaccatgt attatttata tggtattttt ttgcaaaaaa aataccctga aacacaatat
      241 cctgattttg atttttatgc tgtactgttg gaaagttttt gaattgactg gtacaggtgg
      301 actaacagat attttttata gacctgatac aggtaggaaa ttttctctag tacagggcag
      361 tgttaatctt gtaccattca gtggactaaa cattagcttt atattaaata tagtaatgtg
      421 tgtgccctta ggattcttac taccaatcat atggaaaagt tataggaaaa tagttcctgt
      481 tttcttggct ggggctctat tttcactaat tattgaagtc tcacaaatta taactacaag
      541 agcaacagat attgatgatt taatagcaaa tacctttggt gcggttatag gctttatact
      601 gtgggctgtt ttaagtagag tggtgtcctt gaaggacaag tcatctacag tagattattt
      661 agaaccttat atttatatat tgattggttt taccggaaaa ttcttactgt attttccttt
      721 ctggtttgct tttgaagtat tatcctgata attccataga aaataatgag tttgagttga
      781 gaaagaatat tcttgtacac aactctggaa aggaggaagt atgaaaagat tcttaaaatg
      841 ggggcttgtt cttttagcgt ctttgattgt gattctaatg tgtacagcgg ttgttatttc
      901 ttatgtgaat cggccgtatg caagagctgt ttatgatagg attaaacgca ttcttgacaa
      961 agatttttat gaccatctat atctaggttc ttcatttgaa ataataaacc ttgatacaat
     1021 ggatattcat agctatatga aaaaagacca agaagaatta cccgcctcat tgagcaaatt
     1081 atttacgatt gattatgcgc ttagtgtcct aagtttagat gaacagatta caattagtcc
     1141 tactgcactt gacatgcaga aagctgactc atctactgca catcttcaag aaggagatat
     1201 tttatctgtt tatgacatta tctgtgctat ggtcttaccc tcaggaaatg atgcagctta
     1261 tagtttggca atttatacag gtggaaaact tgatcctgat gcaaagaatg atgaagaacg
     1321 ctatcaaagc ttcatgaaaa acttagatcg ttatctaagt gagcaaggct ataaacatac
     1381 tcattttact gacgctgctg gctatgactc caatgctaca acatcagcgg aagatttgac
     1441 aaaagtagct gcaaagctac taaagaatga taaatttgca gaaatagttg ccaaggaaac
     1501 ttatagagca aaatcaatta agggaaatgt ttataactgg aatactacga atcaatttat
     1561 gcaacaagat aatagatact ttactgaagg tgtaaaaggt gtaaaaactg gatcgtttga
     1621 tgggagatac aatctatgtg tgtattatac atctagttct gataaccact atttacttgt
     1681 aagttttggt tcagagagtg atagtaggcg ttatgtagat gttcaaaatc tattgcataa
     1741 tctgccttga aatttcgtgc ctttaagcgc gtatctaaag tttctttgaa aagtttgaga
     1801 atgaagaaag tgagcagtcg aaaaacggtt gctcactttt ttgccctaaa atcgaaaaaa
     1861 tataaaaaag tcctttacaa cacgtctcag gaaaaactgc taagtcaatt ctaatatctt
     1921 gtggagcaag acttgcacct tttgatataa gagagcttag agaactaatg agcgaagatg
     1981 aactagaact cgataaaatt ggagatagaa aaactgcctt gtttgtaata atatcagata
     2041 cagatgatac atttaatttt gtagtttcaa taatgtattc tcaattattt aatctactat
     2101 gtgataaggc agatgatgtg tatggtggaa gacttccagt tcatgttagg tgtctacttg
     2161 atgagtttgc aaatatcggt ttgattccta aatttgaaaa actaatagcg acaattcgtt
     2221 caagagaaat atcggcaagt ataatactgc aagcacaatc tcaattaaaa gcaatttata
     2281 aagaccatgc agatacaata gttggtaact gtgactctac actttttcta ggaggaaaag
     2341 aaaaaacaac agtaaaagaa ttatcagaaa ccttaggaaa agaaaccata gacctatata
     2401 acacatcaga aacaagatca aatcaaaaat cttttggtct aaattatcaa aagactggca
     2461 aggaacttat gagtcaagat gaaataactg taatggatgg tggtaagtgt atttaccagc
     2521 taagaggagc aagacctttt ctatctgata aatttgatat tacaaagcat aagaattaca
     2581 aattgttgga agattatgat aagaaaaact tgtttgatgt agaagagtat ttaaaacaaa
     2641 gatagcgtaa aatattttgt gtaaataact cgtccttctg caaaataatg ggttactcag
     2701 taaacattga agctaatgta tccgtcactt gttggaagcc tttatggctt ctatttaaga
     2761 atttttgatt gtatgtatca aagatggata ctaggaaacg ctctagtgat tcttcatttt
     2821 gaaactgctc ttttctgtgg ctgtatttct tgatttgctt attaaaggat tcaatcaagt
     2881 tcgtagagta aatagtcttg cgtatggcta atggaaaatc atagaaagtg agcaggtaat
     2941 cgttcgaaag gatcgattcc accatttttg gataactggt tttccatttt tccgcaaaag
     3001 caccacgtgc ttccaatgcc gcctttttag atgacgcttg atacaccatt ttaaaatcat
     3061 cacagacttc cttacgatca tcgacacgca ctttgtgact gatattacgg gaaacgtgca
     3121 cacagcaatg ttgaaaacgg gctttggggt agaaccgctg cacggcccct gccatcccct
     3181 ttaagccatc cgtgatgaaa aggaggacgt ttttcacgcc acgttcctgg aggtctatta
     3241 aaatctcttc ccaaatcgtg atagattcag ttggtgcaat cgcatagctc aacacttcct
     3301 ttgaaccgtc cgggcgaatg ccaactgcga tatgaatggc ttccttggcg acggttttcc
     3361 gctttaaagg gatataggtt gcgtccatat aaatagcggc ataacggtca tgaagctcac
     3421 gttccttaaa tgaagtgact tcttccgtaa atgatttagt catattggac atggtttggg
     3481 gcgtgtagtg atgcccatac attttctcaa tcaagtctgc gatttccgac atggtaatac
     3541 cttttcggaa gaggtgaatg acggtctcct ctaaggtatc attcgtccgt ctataagcag
     3601 gaacagtctg ttgcttgaac tcgccgttgc ggtcgcgcgg aatctggaga tgaagttccc
     3661 cgtactcggt cttgaccgta cggtcatagg agccgttacg agaattaccc gtgttaaaac
     3721 caatgcgatc gtacttttcg taatcgagga aagccgttaa ctccgttttg agaagcgtat
     3781 tgacagcttt ttccaaatgg acacggaata attcattcaa atcgccttta ttcgctagag
     3841 tctttagaat ttctgtagta aaatcgttca tatggaagtc ctcttttctg tgaatgtgtt
     3901 gtggtaactc tattctactg aagggacttc cttttttcta tttacacaaa atattttaca
     3961 ctctcggatt gaaagaacgc ggtctttcag gagtaaagat ggtcatctcg gatgcgcata
     4021 tagggctcac aaaagctgtg aaagaatacc ttctttgtgc gagctagcag agatgccaag
     4081 cacatctcct tcgcaacatt tccgacaaat tctccaagaa aatctcaagc gatatcaaag
     4141 aagactttaa agccatcttc aaaacggcaa gcatcgagct ggctggagaa cgtaaacaac
     4201 ttattatgga taaatatggg gaagacaaga aagtcgcaac tgcctgtaaa atattggatg
     4261 agggcttcga agatgccatg caagtaatgg ttttaccaga aaatatccgt caacgattga
     4321 gaacaacgaa tgttcttgaa agactaaatg aagaagtacg ccgtcgtgaa cgtgttgtcc
     4381 gaattttccc aaattgtgat tctgttattc gtatcatcgg tgctttattg atggaaaagg
     4441 atgacgaatg gacttctgca cctagaaaat atcttgaatt tgatcgtaac gagatttagc
     4501 acattaagcc gccaatagta taatatactg gtgagaagta tcaagagacg cttcgctttt
     4561 tgctcttgac acttcttacc agcatattgg accgtagcta ggctcaacaa ggcaaaacat
     4621 cgtactttga gatttccttt ttacacaaga ttttggactt gacacagccc ctatggttct
     4681 taccttgttt atgcttattt aaatcttact ttattacagt actttcaaac ttcgttttgt
     4741 aagccgggta ttttgatatt tcataattat gtccctcttt ttgaatatat tctttgatat
     4801 ctgcttgttc atctacactt tctttgcgta aaatacgttc gatcattgag cagcagtgca
     4861 tgtaaagcaa gaacaattga ttgtttgaaa attcgatccc cattagtttt tcgagttctt
     4921 tgacggcctt ttgtaccaat ctcaaaacct tcattgcatc aaggatcgtt aaattttcta
     4981 cgatcttgtt aatcgagaaa ttaaagtatt gagcacaatt ataggcgata aatggagcat
     5041 ttgattctat cacctgttgt tccttggcgt attcataaat tttttctgcc aaaaaggttt
     5101 ttcctgaacc gcttacaccc atcaataaaa tcggtaatcc gttatgagga tacaagacag
     5161 ccgtcttgat ttgatcgatc gcttccttta a
//
LOCUS       DACDUU010000122         5152 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.122, whole genome shotgun sequence.
ACCESSION   DACDUU010000122 DACDUU010000000
VERSION     DACDUU010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.122"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(4..462)
                     /locus_tag="IWL30_002737"
     CDS             complement(4..462)
                     /locus_tag="IWL30_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9707438.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKLTRV"
     gene            complement(769..1866)
                     /locus_tag="IWL30_002738"
     CDS             complement(769..1866)
                     /locus_tag="IWL30_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP9707439.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            complement(1974..4001)
                     /locus_tag="IWL30_002739"
     CDS             complement(1974..4001)
                     /locus_tag="IWL30_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAP9707440.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            complement(4146..4877)
                     /locus_tag="IWL30_002740"
     CDS             complement(4146..4877)
                     /locus_tag="IWL30_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP9707441.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
BASE COUNT         1459 a         1043 c          814 g         1836 t
ORIGIN      
        1 attttatact ctcgtcaact ttctcttttc aaaaaaaatt tgatattttt ttagatttct
       61 ttttagtctt tgtaatactt tataagccac agaataactc acgtgatgct gtttagcaaa
      121 attttctact gagatatatt tttcctgaaa gaaagccaat accattttca tagataaaga
      181 atccgttata taacgagata acagagcttt gctagaacat tttccattaa tttccagaca
      241 tatatccaat tcactgacgg ttactcgcaa atttttctct agatcgaact tttgtaaatc
      301 atgtttcagc ttatcgatcg tttttttcaa gagaaatgtc gatagcgaca actcttcttg
      361 aacattcgat attgactctg ataaatttga gcttgcttct aaatatttaa gtaatgaata
      421 acttctgtga tcttctttgc ttaaaaaaaa ctcataattc attagtcatc agctctttct
      481 acgtatatta ctttccatgc attcaccaac tagctgggga taaaactgat ttcctggatc
      541 cgagtcactg ttgtcagcga ttcttgtttg tataatatac atcgtcctgt ctttttttta
      601 acattttgga gagcgtttta tttggccttt ctcgcgatca aacctgttat tagaattata
      661 tcatttacta gtatgcattt ttattaataa aatggtatat tttatattgt tataaaaaga
      721 gcccaaccat cctacaataa taaaaattta ctatttgtta tcttaacctt acttttcttt
      781 ctcttctttt tttctcaaaa caaacaaaag tatgcataat aaaataaaca ataagaaaag
      841 tgaagctact aagatataaa tccaattatt gtctttttta atcgtaacat ccgtcgtgtt
      901 caattccttg gctacttttc cgtctattgt aaaatctttt tcaaataccc attgattggt
      961 atagttgatc gtagtatttc ctttttttct cgaaaatttt ccattttcat tttcatttcc
     1021 taaaacagtc attgtaaggt gatattcacc tggggttagc ggttgaccat ttaaagcagt
     1081 tggaaaagaa aaatttgtat tcggcgcaat ctgcaatcct tctttttcct cttgatataa
     1141 aacttcagaa tgtccttttt ttgtaatttt tgtttcaata actacttgat tgatataggt
     1201 tttttgatca ttttgaacgt ttgctaagat gacatttctt gcattgattt gatctggttt
     1261 aacttcatga agagtaaggt tcggagcaac ttcgttcagt gtctgttgca tcaacaaagc
     1321 aataatatag gtgtattcat tctcaatcga taagctttga tccttagttt ctgtttggtc
     1381 cttttccgtc tggatttctt ggaatgtgat cccaccagcc aatacaccat caaatcgctc
     1441 attcggcatc ctgacatgaa aagcaacggt ttggacactt ttaggtttta gcgtcactat
     1501 ctgtggatag ctcacataat ctaccagatc aaagatcagg ctcatgtctt tttcaatctc
     1561 gttttttccg tattcaacca tcacatttga attggtggtt gcgctattca cagaaatatc
     1621 gattttcact tctttttctg tatcatttct caattccgct tttacttcta cttcctgatc
     1681 cggatcaagt tgaagatcga agtaagtttt tctcttgtcg atttgattct ctgaagttat
     1741 cggcgtcact gaaaaattaa atgcactagc tgacactgat atattgttaa aaagtgcccc
     1801 taaaataact aggagtgtta taaataattg ttgttttggt ctcaagaaaa tcgctccttt
     1861 aatcatatgt aagagaagac tataacaaaa tcttgtctct gtgggacaca agagcctatg
     1921 gtacctgtgc ccttcttgaa actcaataat tttgttatat tcttctcttc ctgttaattt
     1981 cctgggacat ctgcaagtat ccaagatagt ttcccctcaa aggttccgat tctttggttt
     2041 tctacaggaa tatttattct gattccacga tgagctgtag aatctaaaag agaggacaaa
     2101 tcgctttcac ccttttgatc actttcgaaa atcgtaacag cggatgtccc tataaccgtt
     2161 gtattttcag aggtaacaaa ggaaagagct tctggaagct caaaatctcc atttgtaaga
     2221 actttctctt gtttcaattg taatttccaa cttccaaaag cccgttcatc tataattttc
     2281 aacccacctg aaaactgtcc gaattcactt aatggtctac tggatatttt atgttttcca
     2341 aaattgacat attgaggagc agttgagaga taaagtttgc cttttctgta ataaaaatag
     2401 atatcctctg tattttcgga gaagatgcct tctgtattcg ctgaatccga gtcaaaaaca
     2461 tacccttcaa tatcaatagg agtttcttta tacttctctc ctattttccc agtgattgtt
     2521 ttcatttcag caatatcaat ctgactgtct ttatctttat aataaacatg gatatctatc
     2581 tccttcagct ttgcgatctt taggtttatt tcattcgatt gctcaatcaa attcccgttt
     2641 tcaaggtatt caaaatgcat agtgagtact ttttcaggaa ttacttcctg caaagttgtc
     2701 tccaattcga ttattaacgt atctccagca ataaaataat ctgtcagatt tagcgtgact
     2761 cgatttccgg ttatcaagat atctttggca tcaatttgat ttccattgat caagatatgc
     2821 tcgggaatag ttaattcttg tgggaggtcg atactagtct gcattttctt atattcaaga
     2881 aaatccccct cgaccttcaa tgtatttctg atagttatct tatcgtttcc tccagcttca
     2941 gcgatggccc cataatcact atcgttgact tttacatcag ctgttgatgt gacattcgat
     3001 ggaagtcgcg tgacagcgac agcattttct tgaaaaaaag tacctgttga agaagtaaac
     3061 ccccaattta cttctgtaga accaaactgc ctaatgagat caggtataat ccgagaagag
     3121 cgaccgacac cttccaaatc ataagtaaga cttctttgct ccgcgtccca tttaattatt
     3181 aacaagcgcc atgtgccatt agaaagaaat gtaggagcta ccgtcacgcc agtatgctct
     3241 ccagaataat tattattatt tggtttaggc gtgacaaaag cgacatgtcc atgccctcta
     3301 ttatcagcag ttatctctcg atccattcga tcagaagaac cactattttt atacgtatca
     3361 aactcaatcg agagcgcatt cgcaatgtaa ggttggccag aacgagtaga atatgagcca
     3421 attcccatac ctggcgatcc aataacttga ctgggattac tagccattct aggatcgcca
     3481 gtcaatgtaa aggtcatgcc atctcccgct ctatctcgtt gatttcctaa gtataaataa
     3541 gatttaaatg taaaatccga gagtaaacta atcttcttat ttgaccaaat agctcctctt
     3601 tgagaccttt ctgctgggtt caactgcagt aacttcccat tttccaatat ggacgtgcta
     3661 gtaccgatag gtgtttgaaa aattttatcg atagaaataa tattgggatc gtcaggcagc
     3721 tcaaccgcag aaattggtgc atcttctagt ccttgtttgt ccgaaccctc agcttctacg
     3781 ttgtcagtgg tctctccact actcgaaata cttaaatcct cactgctttc ttgtgtctgg
     3841 ttggattcta ttaaactgtc gaatgtcgaa gtgagtttgc tttcttcgtc tcttctattt
     3901 ccatgctcca actgattcaa ttccaaagag taccctatga tcggaaagat attagtcact
     3961 aagagactgc ttaaaatgag cacgatcctc ctctttttca atatatcttt tcctcctgat
     4021 cgttaaattc ctagatgaac tattcgtatc caaacaaatt tagcctagcc tttgacaatt
     4081 tttgctcgat tacctcgcaa gacaatatga ctacgtattt atttttaaca gttcttttca
     4141 gtaaactagt tgttaggtgt atcttctaat gtccaaacta atttagtgga atattgttgc
     4201 gctaacttga cagatttacc gggtaccgaa agctgtaccg catcttgatt ttcattttca
     4261 tttgcaccaa atcgatagac ccaagtcccc acacctgttc ctgatttagc attaattgct
     4321 aacgtattgt ttcctcccgg tgtcaacgtt aattcagaat tcaccgtttg cggcgtatat
     4381 gttggatcaa ccaaagaaac agcctgccct ttcgtaaatt tcagctgtgc tccaactagt
     4441 tcatccccac tggcagtttt aaactgttct ggttgagaga cacttaattt ccacccagat
     4501 aaggtacttc tcctatctgt cacttggaca tatgtcggtt ttctagaccc atcgttcatc
     4561 acatcagcag ctgcataata cgttttatcc gcagtagaaa ttttttgagt cccaaatttg
     4621 aattttgatc cataatcaat cgataatgcc cctcctgttc cagggcttgg aggatctgat
     4681 ggatcgacag gtttattggg atcaggattc tctggatcaa caggaggagt tacaccctca
     4741 tctcctgctt caaatgtaat cgttccttct gtgttatatt cctttgggta tacttcagtt
     4801 gcagaaacaa aagtcgtact tgctaccatg ctgaataaca tcattgctgc tgttaatacg
     4861 atttcttttt tcatcattga cattcctctt tctttattta ctaaacatat cagtcgattg
     4921 gattaatcca tagcaaaatt ctgttttacg atacttatga aataaaagaa catagaccta
     4981 tatttggtcc tatgtatcga ttagttataa atgattatca gcccgatttg atttctagtt
     5041 tttattgcaa cttgatgcaa acacaatcta gaaatacagc tgtcatagcc tttaaaataa
     5101 ttcgtaagtc cccctcctat ctatcaaaat gtgacttgac gagagtgtaa aa
//
LOCUS       DACDUU010000123         5147 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.123, whole genome shotgun sequence.
ACCESSION   DACDUU010000123 DACDUU010000000
VERSION     DACDUU010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.123"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(50..739)
                     /locus_tag="IWL30_002741"
     CDS             complement(50..739)
                     /locus_tag="IWL30_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP9707442.1"
                     /translation="MESEDLLSRISEVHPNQVWLDKEKVEKRYIYLFFKRMIDIVGSI
                     IGLILASPLMFYVAYRIRKEEPGSPIFFCHTRVGKNGKTFKMYKFRSMCLDAEEKLEE
                     LLDQNEIEGAMFKMKEDPRVTKIGKIIRAKSIDELPQLWNVLKGDMSLIGPRPPLERE
                     VAEYTRYDLQRLYVKPGCSGLWQVSDRNNVSFHKMVELDLEYISHRSLMYDLRLILKT
                     FYVIVKPNGAY"
     gene            complement(784..1548)
                     /locus_tag="IWL30_002742"
     CDS             complement(784..1548)
                     /locus_tag="IWL30_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAP9707443.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIDRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEQLFFELLQLGKTPVIVHPERNAHFRKDPNHMLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(1576..2274)
                     /locus_tag="IWL30_002743"
     CDS             complement(1576..2274)
                     /locus_tag="IWL30_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAP9707444.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENMSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(2287..3066)
                     /locus_tag="IWL30_002744"
     CDS             complement(2287..3066)
                     /locus_tag="IWL30_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAP9707445.1"
                     /translation="MEETISLKEIFETLRKHVITILISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQATSTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAPTQPKATNDKTAPSRRSRSRV"
     gene            complement(3082..4026)
                     /locus_tag="IWL30_002745"
     CDS             complement(3082..4026)
                     /locus_tag="IWL30_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9707446.1"
                     /translation="MAKRRRRSRGKKIFLIILAILVVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGHDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYRDAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(4067..4846)
                     /locus_tag="IWL30_002746"
     CDS             complement(4067..4846)
                     /locus_tag="IWL30_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707447.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQHHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSLIEQIRNEKIRAAYASKAETVAQQMGYSNTSY"
BASE COUNT         1343 a         1075 c          914 g         1815 t
ORIGIN      
        1 atgaacatgt gtaatctttt ttattaacaa tccatcattc tatccctaac taatatgctc
       61 cattgggctt gacaataaca taaaacgttt ttaaaatcaa cctcaagtcg tacattaaac
      121 ttcgatgact aatatactct aaatccaact caaccatttt atggaaacta acattatttc
      181 tatcactaac ttgccacaaa cctgaacaac caggttttac atacaagcgt tgaagatcat
      241 atctcgtgta ctcagctact tccctctcca aaggtggccg tggaccaatt agactcatat
      301 cgccttttaa aacattccat agctgtggca attcatcaat gcttttggca cggataattt
      361 tacctatttt agtgacacgg ggatcttctt tcatcttgaa cattgcacct tctatttcat
      421 tttgatcaag taattcttcc aatttttctt cagcatctaa acacatcgaa cggaatttat
      481 acattttgaa tgtttttcca tttttaccaa cacgtgtatg gcaaaaaaat atcggacttc
      541 ctggctcttc ttttctgatt cgataagcta catagaacat caaaggagaa gctaaaatca
      601 aaccaataat gcttcctaca atatcaatca ttcttttaaa aaataaatag atatatcgtt
      661 tttcaacttt ttctttatcc aaccaaactt gattaggatg tacttcacta atacgagata
      721 ataaatcttc actttccata ctttccctct ctcctcattt cctttttatc tacgaataaa
      781 gaactaaaat aaaccaaact tcttcttttt tacctcttca tagactggat aactgatttc
      841 atccccgttg atcagatcct ttgcgacttg tttcatctgt actacttttt ccttaccaaa
      901 gtccttagca atctgctcat agcactcttt taagtaaaag gtacgcttag agaccccatg
      961 ggcatcagaa gcagccatct gcacaagatt atgtttgacc atcattttgg ctgttttctg
     1021 gatgtctttt ccaaatacac cgacataact tggtgcggtg agttgggcca agcatcccat
     1081 atctaggaat ggcagcatat gattcgggtc tttacgaaaa tgcgcgttgc gttctggatg
     1141 gacgatcact ggtgtctttc ctagttgtaa caactcaaaa aagagctgtt ctgcatataa
     1201 cggcacttcc agtgtcggaa attcgattag aagatatgta tcatctaaat ctgtaaataa
     1261 aatttcatct cggtcgatgt cttcgatcag tgttccggta atacgtactt cttgtccttc
     1321 caataacgtc aaaggcaatt gtcttttctc taattctgct tgtaacgaag cgactagaga
     1381 aatgacttgt gatttttcat tgctgtattt tccattatta tgatggggtg tgcataaaat
     1441 atgtgtgatc ccttgttgaa tcgctttttc agccatggcg atactggcat ctatgttttc
     1501 tgctccgtca tcgattcctg gcaggatatg gcaatgtaga tcgatcattt ctcttgtctc
     1561 ctctcttttt atttcttaat tcccgtagta atagccagca tctttgctgt gttctgcccc
     1621 attgtataca acaccaagga ctcgcgcttg taccatatcc agcaattctt ttgctttagt
     1681 caatgattct ttccgtgaca tattttcacg tacgaccaat aaggtgccat ccgttttgga
     1741 agacatgatc tgtgcatctg ttacggcaac tactggcggc atgtcgaaga tcactacatc
     1801 aaatagttgt cttgcttctg ctaaaatctg atccattctt ggcgaactta gtagttctga
     1861 tggatttggt ggtttaggtc cacttggcag aatcgacaaa ttctcgactg gtgttcgttg
     1921 gatctcatct gctacacttc ctgatgaact aagaacggta cttaatccgc ttgcgttgtt
     1981 caacttgaac gttttataga tgacgggctt acgtaagtct gcatcgacta gcagcacccg
     2041 ttgaccagat ttggcaaaaa caacggcaat gtttgctgca gtggtcgatt tcccttctcc
     2101 aggtcctgat gaagtcacaa caatcgtttt aatttgctga ccagctgcgg aggcaaactg
     2161 gatattcgtc cgaatcgtac gatattgttc agcgattggt gaagaggggt ttgtgagtgt
     2221 gaccaaactg actgcatgag tttgggctgc tttttgtttc tgtgttcgtg ccatttttct
     2281 ttcctcctag actcttgagc ggcttctgcg tgatggcgct gttttatcgt ttgtagcttt
     2341 tggttgtgtc ggagctggtc cttttttacg gataccagca ttcaattctt tcgctgacat
     2401 ttctggtaca gtccctaaga tcgggaagcc caatgtatcc gtcacatatt tttcatcttt
     2461 tactgtccgg tcgagtaatt caaataagaa agctagacct acgcctacca tcattcctaa
     2521 cactaagcca atcactagat tcaatttatt atttggcgaa acaggtgtcg tattggcaac
     2581 tgcacctgag atgatcgaga ttttatcgac attcatcacg tctttggcgt tctcttggaa
     2641 tacttgtgac gttgtattag cgatttgttg agccgtcact gcatttgtgc ttgttgcttg
     2701 gatcgagaac atttgtgaat tttgtgactg attgacggag atggcatctt tgatttgtcc
     2761 agcagtcatt tttacattgt cttctgtttc taaacgatct ttgacttcat ttagtactag
     2821 atcactgacg atcatatcct tatacgtatt gatcatttgc aggttcgtat tgacatcatt
     2881 gacatttgca gtatctgatt gcggcagtgt cacgatcaat tgggcttgtg agctgtattt
     2941 tggcgtcaag atgaaaaacg tcacgatgcc tgagatggcc aagccagcaa acattgagat
     3001 gaggattgtt atcacgtgtt tgcgtaatgt ttcaaaaatt tctttcaagc tgattgtctc
     3061 ttccattttt tctccacttt cctatttatt ctctagattc atttgatctt ttaattcttt
     3121 tttcacacga tcaagttctt gatcgctgac tttttggtat gagatattgt cttgcatgaa
     3181 gccttctcct tgcagctgat cttgcttgat cgtaccaaat gcatcccgat aatctaaagc
     3241 aatctttttc atttcatcaa agctcatgtc tgttttcata ttcgactcta gagcattcaa
     3301 aacggattga tagttgctta caccgttcag actcaacact tttttcgcta caccttctac
     3361 gatctttctt tgacgttctt gtctgccgta gtcaccattt ggatcttcat aacgcatacg
     3421 agaataagac aatgcctgat ccccgttcaa tgtgatttta ccgatcgtaa aatcatagcc
     3481 atcttgtgag aaggtgaggt cattattgac ttcgatacca ccaactgcat caacgagttc
     3541 ttttagacct ttcatattga ttgaaacata gtgatcgatt gggatgtcta catatttttg
     3601 tacggtatcc atcgccatgg cagctccgcc gaatgcgtaa gcgtggttga tcttgtcttt
     3661 tgtgtcatgt ccaacgatgt cgacatacgt atcacgagga atgctgacaa tcgttgtctg
     3721 tttgtcttca ggactgactg tggcaaccat catcgtatct gatcttcctt gatcgacccg
     3781 acctaaatct cccgtatcaa tccccatcaa tagaacagag aaagattctt gtttgtccag
     3841 atcaactggt tttgttcgtt ctccttgggt tcgttccact gattcgtaag ttttttcaat
     3901 agaatgtgag agatctagat ataattttgc tcctactcct acgattgcta atactaatac
     3961 gacaagaatt gctaaaataa ttagaaatat ctttttccct cttgatcttc ttcttcgttt
     4021 tgccatagtt tcctccgttt gctagccttg cttgaattct cgcttattaa tagctcgtat
     4081 tgctatagcc catttgctga gcgactgttt ctgcttttga cgcgtatgcg gctcttattt
     4141 tttcattccg gatctgttcg atgagactga gtgcggtcaa atattgatcg taagtagcag
     4201 tagctgttac ttgaccgtct ttcagcatcg tatcgaatag ctgatctagt tgcgtgactt
     4261 gtgcaatttg ggtatcagcg aatcctagat attgcataac gaccgttttc caagcactat
     4321 ccttaaaaaa gctcatattt tcacgtacat gggcgacatc tgcacttgcc aacttttctt
     4381 tgatgatgac atcatccaca gcttgctgcc agtttgatac gtttttagta aataatttat
     4441 tgacagccgt ttggatcttc agttttgatt ctgcatcctc taactgtgtt aagactgctt
     4501 tcttttcttc ttggatcgca gcagcttctt taggtaattc tgattccttg atgtcataat
     4561 catctgctga aagtttgata gaagatagtt tatgtgacaa atttgaaact gtttcttctg
     4621 ttatttcagg atttaggaaa attttttctt ttgtactgta cagcttatct acttcttttg
     4681 ataaagaggt cacggtgtct ttttgattgg tgatggcaga ttctgcgtaa ttgatccgtt
     4741 gatgatgttg ccgctgatta ttgagatggt atccaaacaa gcaaatcaca atagcgcctg
     4801 tgcctaccgc cacagtagcc aataacttga cgaacggctt ttccatagat tcactcctaa
     4861 atattgatga tagataaata aagatgtgat tcattcacat cagaatgagc gctttttatt
     4921 ttaaaagcat aattaaacta atatattcat agaaatattt ataaacaggt tttccctata
     4981 taatcatacc acaaacagtt ttcttgacta gggaaattcc taatttaaaa gtaaataaaa
     5041 cagtaacaag attattatta atctttttca tttttatttc ctcacttttc atgagcgttt
     5101 tgaaaattct agcaaagaat aaaataacaa aaattattaa aatagaa
//
LOCUS       DACDUU010000124         5089 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.124, whole genome shotgun sequence.
ACCESSION   DACDUU010000124 DACDUU010000000
VERSION     DACDUU010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.124"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            24..1958
                     /locus_tag="IWL30_002747"
     CDS             24..1958
                     /locus_tag="IWL30_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9707448.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            2415..2831
                     /locus_tag="IWL30_002748"
     CDS             2415..2831
                     /locus_tag="IWL30_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP9707449.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            2845..3411
                     /locus_tag="IWL30_002749"
     CDS             2845..3411
                     /locus_tag="IWL30_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAP9707450.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            3434..4420
                     /locus_tag="IWL30_002750"
     CDS             3434..4420
                     /locus_tag="IWL30_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP9707451.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(4575..>5089)
                     /gene="scm"
                     /locus_tag="IWL30_002751"
     CDS             complement(4575..>5089)
                     /gene="scm"
                     /locus_tag="IWL30_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP9707452.1"
                     /translation="SDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSDSTDT
                     TSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHA
                     SVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRI
                     TFMSYKKSKR"
BASE COUNT         1529 a          944 c         1130 g         1486 t
ORIGIN      
        1 agtctataag gagcggatga tcaatggcaa agcaaaatat tgtcgttgta ggagcaggat
       61 atgccggtgt ttcagctaca aaatacttgg caaagaaact gaaaaaagaa gatgtagcaa
      121 ttactctgat cgatcgtcat tcgtaccata ctatgatgac cgagttgcat gaagtagctg
      181 gcggacgtgt agaaccagaa gcaatccaat acgatctgca gcatttgttt gctcgtcaaa
      241 agaatgtaca acttgtcaca gacactgtaa tcggtatcga taaagaacga aaaatcgtca
      301 agaccaaatt aggttcctat ccttttgatc agctgatcct tggaatgggc ggagagccga
      361 atgactttgg tacacctggt gtcaaagcac acggattcac cctttggtca tttgaagatt
      421 ctgtaaggat tcgggaacat attgagaaga cagtagcaaa agctgcattg gaaccagatg
      481 atgaactgcg taaagccatg ctgactttcg ttgtctgtgg ttctggattt accggtattg
      541 aaatgattgg ggaactgatc gactggaaaa atcgcctagc caaagattat aaattagatc
      601 caaacgaaat cacactgatg gtcgtagagg caatgccgac gatcttgaat atgttggatc
      661 gaaatgatgc tgcaaaagct gaacgctatt tgcgtaagca caacgtggaa ttattattga
      721 atgcaccaat cgtagaagtg gctgctgatc atatcaaatt gaaagacgga cgcaacattc
      781 cgactcacac actgatttgg acagctggtg tcaaaggaac ttctgacgct gctgactttg
      841 gattagaatc agcacgcgga cagcggttga tcgccaatga atatatgcag gcaaaaggct
      901 acgaagataa aaacatctat atcattggtg atttagtctt ttatgaagaa ttcccaggaa
      961 caccgacacc gcagatcgtt caagctgctg aacaaacagg acacacagcc gcagcgaata
     1021 tcgtggcttc gatcaaaggc ggagaaaagc ataaatttaa aggaaactat caaggattta
     1081 tggtttctat tggtgccaaa tggggggtag ctaacctatt tgataagatc catttaagcg
     1141 gatttttagc tatcgtaatg aaacatatcg tgaatttgaa atacttcttt gatattcgtt
     1201 ctggttatta tatgttccaa tatttaatgc atgagttttt ccatatcaaa gatgatcgca
     1261 acgtgactcg aggacattct tctcgttacg gcaatgtctt atggagtgtg ccacttcgta
     1321 ttttctatgg tttagtttgg ttcgttgaag cttccaaaaa aatcattggg aatggtgaag
     1381 tgttcaaacc aagtacttgg tttggtgaag gatcatggtt cactggaaac agtgtgttcc
     1441 cttggacatg ggaatacaat gtaacagatg ttgccagtgg tgcttctgct gctggtgatg
     1501 caaccacagc tgccagcgga gcaggggcaa gtgccggaga agcagcgact caagctgcac
     1561 attttggttt gagttatgca tacggtgaag aaccaatggc tgtgattgag aagtcaccag
     1621 actggttcac aaagatcatg caagtgatca tgccaaacaa tgatgtagca atgttcttcc
     1681 aaaaattcat ggtattcttt gaaattgcct tggcattagc attgattgct ggattattca
     1741 catggctatg cagtgcgaca acgattgtat tggtgatcac gttctgctta tcaggcatgt
     1801 tttactgggt aaacatttgg ttcatttttg ttgctttcgc attgatgaac ggttccggac
     1861 gtgcgattgg gttggatcgc tgggtaattc catggctaca acgtacacta ggtaattggt
     1921 ggtacggtag accaagttcc cagtacggca agaaataaat agataaagaa cgttatttga
     1981 atgcaagatt tcaatagaaa ggggctgaga caaaccgtca cagcctcctt tttatgtaaa
     2041 attaaaggaa taagatgccc gagataagcc tccatgagca agacacaggt gtcacaacct
     2101 ctgttacaac ctcttgttat aaacggtgtt ccagaagtag cttcttcgaa aaataagccg
     2161 aaatttcaca aaaacttgaa aagtaatttt cgtaaattcc ttcttatttc ttgaagttgc
     2221 ccacttctgt cacaacctct tgttataaac ggtgttccac ctgctgcttc tcggtaataa
     2281 gcccaaagaa ttagaaaaca tggaaagctg ttttctattc ttcgttctta ttactcaaag
     2341 caagacacag gtgtcacaac ctcttgtttt tatgctaaaa ttgggtgagt taatctggaa
     2401 aaaggagaac aaaaatgagc cttaaagaat ttgctgaaaa aagtctgctc aaaccttggg
     2461 atgtttttat tattttgctt ttgactgttc tttctttttt accagtcatg atttttggtc
     2521 ttcagcaaaa caccgcaaca gcagaaaaag aagcggtact gcgtgtggat ggcacagaaa
     2581 tcaaaacatt ccctttgatg gctggagaag agagcaacac atatacttac aaagatgaac
     2641 acggagatta taacttgatc gagatagatg gcgatcgagt acggataaaa gaagcagatt
     2701 gcgatgacca gatctgcgta cgtcgaggct gggcaacgaa aaatggcgaa accatcgtct
     2761 gtttgccaca taaattagtg atcgaagtcc gttcaacaga tggaggtgac gaagacagtc
     2821 tgatttatta gaccttcaca caccatgagt aaattacaaa aaatgattta tatttcgcta
     2881 cttgttgcac aaggagtgat cattggttta ttggaaaata tgatccctta tcctttttct
     2941 tttgcgcctg gtgcaaaact aggcttagcc aatctaatca cgatcgttgc catttttacc
     3001 atgaaaaaac gagacagctt cttactactt tggctacgac tattcctgac tactttgcta
     3061 ggcggaacga tttccacatt cctttatagt atgagtggtg ctttgcttag ttattttgga
     3121 atgttattgg tcaagcaatt aggaccaaaa cgtgtcagta tcatcggaat tagtgcggcc
     3181 ggcggtttca tgcataatgt cggacagctt ttgatggctt ctttcattgc acaatcatgg
     3241 acagtcatgt tgtacttgcc agtcctttca gttttaggga ttttatcagg gattgcaatc
     3301 gggatcgcag cgaattatct tttacgacat gtgcagacac ttcaacgatt ccaaaaagat
     3361 tatgagcagg gaaccaaaca aaaatggagc ccaatgttta ccaaaaaata atcgtcacac
     3421 aaggagtttt tttatgaagc tacatccaat gtgggaaaac tatcctgctt taagtaaaga
     3481 attgacagaa acactaaaac ggatggaaga aactgtccgc ctgaaaaaca aacctgtcga
     3541 acaagcaatc aaagaaacga tccatgccgg cggaaagctg ttgcgtcctg cctatcagct
     3601 attgttctca caatttggac cagagcaaga tcgagacaaa gccatcgctt tggcagcttc
     3661 tatcgaaatg ctccatacgg ccacactgat ccatgatgat atcgttgatg aagcggatct
     3721 gcgacgtggt cagcctaata tccgtagtca gtttggtaat gtcgtttctg tctatgcagg
     3781 agactattta tttgtctgct gttttaaact attgtctgat tactccactt ccttgaaaag
     3841 tctccaattg aattctcgca gtatggaaaa agtattgaat ggcgaattag ggcagatgga
     3901 tgatcgctat aattatgaac tttctgtaaa agaatatttg gaaaatattt caggtaagac
     3961 agctgaactg ttccaattaa gttgttctgt tggcgctttt gaaagtggaa caagcgagcg
     4021 cttcgcaaaa aaagcaggag atatcggact ttctatcggg atggcctttc agattatcga
     4081 tgatatttta gattatacaa aagagagcca ggaaatcggc aaacctgtac tagaagacat
     4141 gcgtcaaggg gtctattctt taccgttgat ctactctttg caaaaaaaca aaccaaagct
     4201 actaccttat ttagagaaga aagcggcaat gacagaagaa gatgtcgacg ccgtacgcaa
     4261 aatcgttgaa catacaggag gcgtggaaga agctaggaag ttagcggcct cttacacaga
     4321 aaaggctttg aaggaaataa agaaattacc tgccacctct cttcgaacaa aagagaatct
     4381 cttttccctt acccaactga ttttggatcg caaagactga atagggaaag gttcgatacg
     4441 taaataaatt tctcagaagg aaatctacgt gttttttgac aaattgaatt gtagttcttg
     4501 tatttatgat aaaaagcaaa aataaagagc aacgtccgtt agcgacgttg ctctttttta
     4561 tcttgaatat ttttttacct tttcgatttc ttataactca taaaagtgat tcttaaaccg
     4621 attacaagta atataacgcc tgcgagtgtt atccaattat tcgattgact tccagtttta
     4681 ggaagtgctc tttttgattt gtttccattg tcattgcttg ttttattagt taagttaaag
     4741 ttcgtcctgt tatccgaact tgtttttgag aaggagacac ttgcatgacg tgtatcgctt
     4801 gtggtagacg acgtgtctgt actatcgctt gaagttgatg aatctgagct atcacttgtg
     4861 gtagaggatg tatctgtact gtcacttgaa gctgacgtgt ccgtgctatc acttgtagtg
     4921 gtagatgaat ctgagctatc acttgtggta gaggtagtat ctgtactgtc acttgaagct
     4981 gacgtgtccg tgctatcact tgttgtggta gatgtatccg tactgtcgct tgaagctgac
     5041 gtgtccgtgc tatcacttgt ggtagaggta gtatctgtac tgtcacttg
//
LOCUS       DACDUU010000125         4829 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.125, whole genome shotgun sequence.
ACCESSION   DACDUU010000125 DACDUU010000000
VERSION     DACDUU010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.125"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..784)
                     /locus_tag="IWL30_002752"
     CDS             complement(<1..784)
                     /locus_tag="IWL30_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707453.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFE"
     gene            complement(781..1536)
                     /locus_tag="IWL30_002753"
     CDS             complement(781..1536)
                     /locus_tag="IWL30_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9707454.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            complement(1549..3525)
                     /locus_tag="IWL30_002754"
     CDS             complement(1549..3525)
                     /locus_tag="IWL30_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP9707455.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGGLSAVVYFKKKA"
     gene            complement(3578..4249)
                     /locus_tag="IWL30_002755"
     CDS             complement(3578..4249)
                     /locus_tag="IWL30_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP9707456.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            complement(4309..4518)
                     /locus_tag="IWL30_002756"
     CDS             complement(4309..4518)
                     /locus_tag="IWL30_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707457.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            complement(4585..4824)
                     /locus_tag="IWL30_002757"
     CDS             complement(4585..4824)
                     /locus_tag="IWL30_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707458.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
BASE COUNT         1283 a         1018 c          730 g         1798 t
ORIGIN      
        1 tttcaaaaaa gttcttcttt acaccagttg acacaacttt tttaatttct tctttcggct
       61 ctgatttctc tgatttaggg tataaccaaa catccgtcaa aaattcacct ttttcatttg
      121 aaacaggtgt caataaaaca atatcttcac tgtttgaaat gttttcaggg cttgattcat
      181 taacgatata gtatgcttgt tttttgtttg caggtacttc aatggaaaat tcaatcacgc
      241 cttcctgatc atcaatcgtt ttggtttctc cttcagccac ttttttcaac tggtcgtatg
      301 aaagtttttt tgctcgatct ttcaattttg actcaattat ttctacagat tgatttttct
      361 cattatcatc ttgtgacatt tcttctaaga tattcgtgat atcgtagaca ataaacttcg
      421 cgccattgat accttcttgc tgggctactt gactgccttg attgttgatt tgattcgaag
      481 aaataatttt ttgaatatga atggcttgct tgatcacttg cgtatcttct acgtcttgcg
      541 gcttttctga agtctctgaa gaactttctg acgttggatt cgttgattca gtaaagctta
      601 tatttgaatc tgccgtttct tctgtagtat tctccgaact gtccgtttcg cttggtgcag
      661 taattgaatt atctattgtt tgagtggtac tactatcact gctgtcagcg aaagacagtt
      721 gcgaataagc tacagtagaa aagagagctg ctacacaaac aaacagccat tttctcttat
      781 tcattccgat ctcctcctta tttttttata aataaaaata ataggaacgc aaaaaaaagc
      841 gaatagcagt gctaatcgcc aatccaatga aattgatttc ctattttttg cttttttctg
      901 tagttttaaa tcttctttac tgaaggaaac tcgttcacca tacactaaaa agcggtgtga
      961 gttgacacct tttggtgtgc atgtcattaa cgtgatccta tcttggtctt tttcaggcat
     1021 aagataatct aaataatttt tttgtaattc ttctgcttta acttcttcaa ttcgataaat
     1081 ttgataggca tgttgttctt catttacctt aataaagaat ttttgccctt tttttactga
     1141 aggcaaatca tcgaaaagat tatctttata aagtccactg tgccccgcga ttaaaggatt
     1201 tttaccgttt cctcctgtaa tatcgccagt tccttccgta atccctacac cattctctaa
     1261 aattttgtcg gaagttccgt cataaattgg tagttttaaa gaaatacttg gaattgtaag
     1321 aacaccaaca ggatcgccta actctatttt cgcaacatcc aaagaagaat ctgttttttc
     1381 aagattttca atggctaaaa ttctttgctg ttgttgaaat ttcgctacat ttgtttccac
     1441 aactggaatt aaatagactg ctactgccga cagcaaccaa aaaaatatca tcagtttgtg
     1501 catgactctt ctttttgggg gcttacgaat aatcattagc tgatccttct aggctttctt
     1561 cttaaagtaa acaactgcag aaagtccccc tgctcctagc aacaagcaag agatcaatac
     1621 gtaagattgg aaaccgttgc taccagtcat tggtacggaa gctttttgat tatttttgat
     1681 cgtgattcgg ttttcttctt tgtatgaatc tttgtctact ttgaaagaga tctcttttgt
     1741 taatttttga tagttttcag gagcttttgt ttcttccagt gagtaatctc cttcagttaa
     1801 accagtgatt tcaaaatgac cctctttatc tgattttagg attgctgcat ttgctacatc
     1861 gtctacccaa actgcttctt tgacaccatc tttatctgta tcagtaagtc caccgtagta
     1921 cttaccttct gcattcttag ctgcaaattc tgcgccttca agcggtgttg ctttgatacc
     1981 tgttccaaat aggccgctat cgacttttaa gaattttgcg ccataagtca caacgtcagc
     2041 tttcgttttt tcgtggtgtt cttcctgatt aaatggatta ttgttataaa cgaaatccac
     2101 gctatttgtt aatggatttg cgatttcagc actatcattc acaaacatgt tataagtcat
     2161 ttttagatct tgaccatgta aattcgcaac tgtatcactt acttgtccat tatttacgat
     2221 aaaatcgatc acaaaaccac tatcagaatt ttcgatttta tagtcagtat tttcttttaa
     2281 tacgactgaa ccatcattat ttgttacttt aatatctgct ttttcagcaa cataagaaag
     2341 tcctttacct gctgtatctc cccatttaaa tacactgtaa ggagaagttt cgcttttcac
     2401 accgtttact gtgataccgt ctgcgccacc tgcaatgtct gttggaatat gaatcgttcc
     2461 ttgataattt actgcgtcac caatttggaa agaatgttct tgtcctgttc catttgttac
     2521 tgttttatcg atcactggtg ctttatagtt tacgtatgaa gcttttaagt cacttgtcac
     2581 gccatcattc gtaatttcaa aggttaattt attgtgcgca tcgttgcgag cgtctgcgcc
     2641 taataaatat tgatctgctg ttggttcttg atctgatccc actacaactt ccgaaggaac
     2701 ttccacaaaa tagtattttc ctaacgtcaa atcagtcgca gtcagctttc cttgatcatc
     2761 cgatacaaga tcacccaatt ttgttccttt tccaggttcg cccttgtata actcaaattt
     2821 tgcccctttt aaaagggttt gtttgctttg gtctgcgcca tcgtcaccaa atttcgttaa
     2881 ttcaaaactc aacttagaaa caatgttctt aggatacaaa tgaatatttt ttaaaaatcc
     2941 tgatcctgtt ttatctgtca ttggcgcaat cacaaccatt ggttttgctt tttgtgtgac
     3001 taatccagca gcggatttac tttcataaac cacatataca ttccctttgg aattgacata
     3061 ggctggttga tcagtaaaag tgatctcacc attttcatct acagctgaag taattttgtt
     3121 ggttgcattc ttgacgtact cactgttaga tcccttgtct tctacatctt gtacaatctc
     3181 gtccactttc gcgttggtta gatcagaatc ttctctcggt aatacttgat ccgtaatatt
     3241 tcctaacgta aactcaacat caccataatc tgccttgttg tatttcgtta cacctgtagg
     3301 atattgatcg tgcgtataac catcgttttt gatcccgtca atatcaacat tcaattgcgt
     3361 tccttgatcg tacattaatt tatgaaccgt gatattgacc ttctctggtg gtgttttttg
     3421 cgtgacatct tctgcatgta cttgataccc ctgaaagcag cctgcaatga ttcctaacat
     3481 tccaacaagc attccagctt tttttacata ccgatttatt gccatttttt actctcctca
     3541 tttcatattt caacgactcg cgttgattaa aaccttgtta ttgtagctct ccctgtacca
     3601 cgtatctttc ttttccttgt gacgtataaa cacaagtaaa caacgttaat aagggtttct
     3661 ctcctttctt tggcaaagac aagaaagagt tctcttcagg aacgccatcg attaatttaa
     3721 agtacccagg tacgataaat ttacgcgtta ccttgtaacg atagacgcca tcgttactaa
     3781 ctaagtccat cacctcccct aatgacaact tttgaatatc agaaaataaa acacctggca
     3841 tttccatgtt atgacctgct aggacatagt ttcctttgcc catttcggca tcttcataaa
     3901 aataagtagc cgctccaaga ctcaaagtgt attgatttgc ccctttaaaa attggcaatg
     3961 ctaaaccaag tgcaggaatc tgaatttgtg caaatacttg tttattgacg cccatttcga
     4021 ttgcttcttt ttgtatggac aacatttgcc ccaaagacat atcattattg acgttcgttg
     4081 catcctcctt ataactcttt gcatgtgcat tgatttgatc tacgggcatc tttttttcta
     4141 tattttgctt aaacacctct ccttcatgtt tttggtagct aacatagcca aacacactgc
     4201 aaaagacgat taaagtgact cctaaaaaac caaataacca ttttttcatc catctgcctc
     4261 ctttcagaca aaacaaaaaa actttttctc atttccatag aaaaagtttt aaaaatgaat
     4321 tctataagtt cgtctcaact gttcaaagtt tttcattgaa cctaacaaac aagctttctc
     4381 ttttcctgtt aactgcatat cattgatata ttgcgcgaca gataatcttt taaggtgttt
     4441 ttgcttcatc gtttttagta acttttcttc catttctttc gacacttttt ttctgttttc
     4501 gatttcttca taattcatga gttttcctac cttccttgtt ttttgcaaaa ataaaaaagc
     4561 ctatgatcac atacgctcta cacttcagtc ttgggttatc tttttctttc gtttaattac
     4621 tggccgtgct ggtttcaaat ccttgattcc attatgggtt ctccaataat cgaattcttt
     4681 tcgcaatctt tctttttcca tatctgttcc tttagataag gctttgaata cttctttagg
     4741 tgaataacca cgagctaaca gctgtacata ccttcctttt gtcaaagtat ccatatccat
     4801 cgcctctaaa actggtcgcg ccatctact
//
LOCUS       DACDUU010000126         4711 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.126, whole genome shotgun sequence.
ACCESSION   DACDUU010000126 DACDUU010000000
VERSION     DACDUU010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.126"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            106..357
                     /locus_tag="IWL30_002758"
                     /pseudo
     CDS             106..357
                     /locus_tag="IWL30_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            450..1244
                     /locus_tag="IWL30_002759"
                     /note="aph(3')-IIIa"
     CDS             450..1244
                     /locus_tag="IWL30_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP9707459.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1654..1765
                     /locus_tag="IWL30_002760"
                     /pseudo
     CDS             1654..1765
                     /locus_tag="IWL30_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1786..1869
                     /locus_tag="IWL30_002761"
     CDS             1786..1869
                     /locus_tag="IWL30_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAP9707460.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1994..2731
                     /gene="erm(B)"
                     /locus_tag="IWL30_002762"
     CDS             1994..2731
                     /gene="erm(B)"
                     /locus_tag="IWL30_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP9707461.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2994..3857)
                     /locus_tag="IWL30_002763"
     CDS             complement(2994..3857)
                     /locus_tag="IWL30_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAP9707462.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3859..4131)
                     /locus_tag="IWL30_002764"
     CDS             complement(3859..4131)
                     /locus_tag="IWL30_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP9707463.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4148..4363)
                     /locus_tag="IWL30_002765"
     CDS             complement(4148..4363)
                     /locus_tag="IWL30_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAP9707464.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4455..>4711)
                     /locus_tag="IWL30_002766"
     CDS             complement(4455..>4711)
                     /locus_tag="IWL30_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HAP9707465.1"
                     /translation="IGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVS
                     TWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1446 a          796 c          865 g         1604 t
ORIGIN      
        1 ttcttgggag ttttgagact actatatgcg tttttttgtt gatttaccaa catgtttaga
       61 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
      121 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      181 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      241 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      301 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      361 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      421 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      481 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      541 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      601 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      661 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      721 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      781 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      841 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      901 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      961 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
     1021 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1081 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1141 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1201 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1261 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1321 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1381 cgcctacgag gaatttgtat cgataagaaa tagatttaaa aatttcgctg ttattttgta
     1441 catttaactt gacggtgaca tctctctatt gtgagttatt agtggtacag ttttcaaccg
     1501 ttttaattat aaaaaagtgg tgcattttta aattggcaca aacaggtaac ggttattgca
     1561 ggtgtatttc ttatctatgg gtttaacatg gattttatca ttaaaatcat gagtattgtc
     1621 cgagagtgat tggtcttgcg tatggttaac cctaaagtta tggaaataag acttagaagc
     1681 aaacttaaga gtgtgttgat agtgcattat cttaaaattt tgtataatag gaattgaagt
     1741 taaattagat gctaaaaatt tgtaattaag aaggagggat tcgtcatgtt ggtattccaa
     1801 atgcgttatg tagataaaac atctactgtt ttgaaacaga ctaaaaacag tgattacgca
     1861 gataaataaa tacgttagat taattcctac cagtgactaa tcttatgact ttttaaacag
     1921 ataactaaaa ttacaaacaa atcgtttaac ttctgtattt gtttatagat gtaatcactt
     1981 caggagtgat aacatgaaca aaaatataaa atattctcaa aactttttaa cgagtgaaaa
     2041 agtactcaac caaataataa aacaattgaa tttaaaagaa accgataccg tttacgaaat
     2101 tggaacaggt aaagggcatt taacgacgaa actggctaaa ataagtaaac aggtaacgtc
     2161 tattgaatta gacagtcatc tattcaactt atcgtcagaa aaattaaaac tgaatactcg
     2221 tgtcacttta attcaccaag atattctaca gtttcaattc cctaacaaac agaggtataa
     2281 aattgttggg aatattcctt accatttaag cacacaaatt attaaaaaag tggtttttga
     2341 aagccgtgcg tctgacatct atctgattgt tgaagaagga ttctacaagc gtaccttgga
     2401 tattcaccga acactagggt tgctcttgca cactcaagtc tcgattcagc aattgcttaa
     2461 gctgccagcg gaatgctttc atcctaaacc aaaagtaaac agtgtcttaa taaaacttac
     2521 ccgccatacc acagatgttc cagataaata ttggaagcta tatacgtact ttgtttcaaa
     2581 atgggtcaat cgagaatatc gtcaactgtt tactaaaaat cagtttcatc aagcaatgaa
     2641 acacgccaaa gtaaacaatt taagtaccgt tacttatgag caagtattgt ctatttttaa
     2701 tagttatcta ttatttaacg ggaggaaata attctatgag tcgcttttgt aaatttggaa
     2761 agttacacgt tactaaaggg aatgtagata aattattagg tatagggcac ctctaataac
     2821 taccaattaa ttcacctcaa aatgaaatta gaaaaaaaca gcgaaatttt taaatctatt
     2881 tcttatcgat acaaattcct cgtaggcgct cgggacccca acatgaggaa atgtttttat
     2941 aaaaaaagag tagttaccgt tttttggtaa ctactctttt ccttttttaa ggtttaaata
     3001 cctggaagtt taggtgtttt gggtattggt ggtgtaggtg gctgcaagct ttccaatttt
     3061 tgttgaattg ctttaaattc aggtgtctct tggtgtttat ttagaaccat tttttgctct
     3121 attcgttcta aagtcggttg aatttctttc cctgatactt tacgattcaa ttctttttct
     3181 aaggtttctt ttggactaat agaaggtgtt tccaaacttg aatagagttt tactccttct
     3241 ctgttatata gccttatatc gctaaataag cccgttttat gaagggtttc taaattggtc
     3301 ggtaagtttt tgacaacaat atcatgcgct tgttttggtg ttgccctggc tgtcattgga
     3361 tcatctgcat acatggtttc atatcgttca attgttccta aatatgagtt gattttaggt
     3421 actgccatga catacatttt tgtttcataa cctttggctt gaagcattgt tgcggtttga
     3481 ataggaacgt ctgttgttcg tcctgtacct tcgatcacca aattataccc ttgatcgctc
     3541 aaacggctta tgatcgcttc tgtcatgcga ttagaataag gggtaacgtg ttttactacg
     3601 tctttttcat aaagtttcac tagttcatca aaattagggt gctgttgttt aaaggtatca
     3661 ttatcaatga caataacatt cccttgtgtt tcttcaaaaa ttgctgatcg caaactggtt
     3721 ttccctgacc ctggttgccc accaagtaaa aaagcggttg gcgattcaac cgcttttttt
     3781 ccttgaatca attcttctaa attatcattt aagcgattct caaattgttt gtcagtaaaa
     3841 ttgactatat ttgccatatt aagccacttt ctctttattc aaaacttgtt ttgagtaacg
     3901 attcattgac cgccaatact catgaacggc ttgtaattct tctaaagaat aatcaaaaag
     3961 atttacacgt tttgcaagct ttaattggtt taataaattg acttccaata attgagagtc
     4021 atttttattt aattcatggt tcaaaacata gtttaatact cgattataaa cgcttgccga
     4081 taattcgtta atgatctcta tttcaaatgt tttttcatac gtaactgcca tttcattttc
     4141 actcctttta aagtttgtca ggtaaatatt tccgtatata ttcttctaag gcttgatcca
     4201 taacttcttt tacgtttccg ccattctttg ctgtttcgat ttttatgata tggtgcaagt
     4261 cagcacgaac acgaaccgtc ttatctccca ttatatcttt ttttgcactg attggtgtat
     4321 catttcgttt tgctttttgt gcgcctaaat ttcccacaat cactcacttc tttctatttc
     4381 ttcttactct tattttatca tcaacaatca caaatcacaa gtgatttgtg attaatcact
     4441 tgtgatttgt gattctattc tttttcgttt tctaattgaa taattcgctc aagcatttca
     4501 aaaaatacgt tcttatacat ggataaaact tttttgtcat agcctttatg ctctgtaatg
     4561 ccgtttttag accaggtact tactttatta cttcgcttga taatattttg gaaaaccaag
     4621 ttatcttctt tatgttgctt atacagttct tccaggtttg attttatcgt tgcgctgtcc
     4681 gtatcaacta aataaggaac aaaaccgatc a
//
LOCUS       DACDUU010000127         4671 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.127, whole genome shotgun sequence.
ACCESSION   DACDUU010000127 DACDUU010000000
VERSION     DACDUU010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.127"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            59..913
                     /locus_tag="IWL30_002767"
     CDS             59..913
                     /locus_tag="IWL30_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003065868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9707466.1"
                     /translation="MENSIYLVTEPNTQLKDCYFNFCGYSKTEPRHSFGPAIREQYLI
                     HIVLEGEGYYSINDQKYFLKKGQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYY
                     LKEIGVGKNHLTFKVENLNSFKGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSAS
                     YLNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISI
                     KEYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRL
                     GLGIINGE"
     gene            1010..3259
                     /locus_tag="IWL30_002768"
     CDS             1010..3259
                     /locus_tag="IWL30_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP9707467.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3262..4437
                     /locus_tag="IWL30_002769"
     CDS             3262..4437
                     /locus_tag="IWL30_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9707468.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRVTRGAS"
     gene            complement(4440..>4671)
                     /locus_tag="IWL30_002770"
     CDS             complement(4440..>4671)
                     /locus_tag="IWL30_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP9707469.1"
                     /translation="KNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVKGL
                     ESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1631 a          672 c          845 g         1523 t
ORIGIN      
        1 gtaataatat aaacataata aaacaaaaat tatccaaatc taacatagga gggtggatat
       61 ggaaaattct atatatttag taacagaacc taacactcag ctgaaagatt gttactttaa
      121 tttttgtggg tattcaaaaa cagaacctag acatagtttt ggaccagcta ttcgagaaca
      181 atacttaata cacatagtgc tagaaggtga aggctattat tcaattaatg atcaaaaata
      241 ttttctgaag aaaggccaag gatttgttat cccaccagga gtatctactt tttatcaagc
      301 agatgaaaaa aatccgtggt catatgtatg gttgggttta agtggcaatc ttgtagaata
      361 ctatctaaaa gaaattggtg ttggaaagaa tcatcttacc tttaaagtcg aaaatttaaa
      421 ttcatttaaa ggattaattt ttgaaacttt atcgtatgat caggacaatc tagtcagtga
      481 attgtccaga caaaaacaag tttataaatt tttagaactc ctcttaaatt cagctagtta
      541 tctgaacgaa gggataatta ctaaaaagat gaatcctttt gttcgtcaaa caattgaaat
      601 aatacgtgag aatgcgtatc aaaaattatc tgttaaagat attgctgtca gtctatcgat
      661 acacgaaaat tatttatcac gattgtttaa gaatgatatg ggaatatcaa taaaagagta
      721 tattagtgaa atcaaaattt ctattgcaaa cgatctgtta tcatcaactg aatattcaat
      781 ccagcaaatc agtgacaaga taggattctc aacagtacaa tctttttcta aagcctttaa
      841 gaaagcaaat ggtgtttcac cgggagtata tcgaaaacgt agattaggtc taggaatcat
      901 taatggtgaa taaactaaac atttgatttt gataaatatt cgttacttta aacgcttact
      961 atttatgtga gcgtttttta taatgcaata aagaaacgga ggagagctta tgtataagga
     1021 taatgaaatg aatattattt atcgatcaga aaaccaacaa tttcatttgt ttaatcagga
     1081 gataagttac atttttaaga tatcggaaga tggtaaatta ttgcatttat attatggtga
     1141 gaacttacca gataatgact atagtcatct aattgagatg catcatcggc ctatgacaac
     1201 ctatagaaat gaaaacgatt tgctatattc gttagagcac ttaaaacaag aatttcctga
     1261 atatggtaca acagatttta gacatcctgc tatttcatta ctacagaaaa atggctctcg
     1321 cttatcagat tttgtctatc aagggtacga agtggaagaa ggaaaaccta aactagaagg
     1381 tttacctgct acctatgttg ataataatga tgaaagcaaa acattgaaag tttatatttt
     1441 agattcagtt acaaatgttc aagtagaatt attatataca atttatagag attatcctgt
     1501 tattactcgt agcgcgcgag ttatgaataa aggaaaagat attcaaataa tcgagaatat
     1561 tagcagttta tcattggatt tacccgatgc aaattatgaa tggcaacaat tatctggtgc
     1621 ttggggtaga gaaagaacta ttaagtcgcg tactttacaa caaggaattc aatcaatcga
     1681 atctactcga ggtatttcaa gccatcagca taacccattt gttactttaa aaagaaaaaa
     1741 tactgatgaa aatcaaggag aagctattgg tgcagcacta gtttactcag gaaacttctt
     1801 aattcaagca gaagtagata catgggatgt tactagacta caagttggca tcaatccatt
     1861 tggatttaat tggaaactca atccaggaga aatatttaca gcaccagaag cgattctggt
     1921 ttattcaagt aagggtttaa atgccatgag tcaaactttt catcaactat ttagaaaacg
     1981 cttagctcgt ggtaagtggc gagaaaaaga ccgtcctgtc ttaattaata attgggaagc
     2041 aacatatttt gattttgacg aagaaaagtt ggtaaatatt gctaaaaaag catttgatgt
     2101 tggtattgaa ttatttgtat tagatgatgg ctggtttgga gaaagagaaa acgattgtgc
     2161 aggattagga gattggcatg taaatcctaa aagactacct agaggaatcg gcagcctttc
     2221 agaaaaaatt cgtagttttg gtatgaaatt tggtttatgg tttgaacctg aaatggtgaa
     2281 taaggatagt gagttatatc gcacacatcc gaattggatt atttctacac ctaatcgaaa
     2341 acctaatcat ggtaggaacc aatatgtatt aaattttggg ttagatgagg ttgttgaaaa
     2401 tattttcgat caaatgtgta aaatcattga tgaatcaaac ttagattaca ttaagtggga
     2461 tatgaatcgt ccattgactg atgtatttga cagtcacttg tcagcagatc aacaaggaga
     2521 agtattccac agatatgtac taggcgtata tcgcttatat gaaaaactta taacaaaata
     2581 tcctaacata ctatttgagt cttgttcatc aggtggtggg cgctttgatc ctggaatgct
     2641 tcactatgca cctcaagctt gggctagtga tgattcagat gcaattgaaa ggttaaaaat
     2701 tcaatatgga actagtatgc tgtatcctct ttcgtctatt ggtgctcatg tatcaattgt
     2761 tccaaatcat caaacaaacc gtataacacc tataaaaact agaggaaatg tagctttttt
     2821 tggtgcattt ggatatgagc ttgacttaaa tgaattaagt gaaggcgatt tgaggattgt
     2881 aaaagaacaa attgcctttt ataaaaaata tagaaccata attcataatg gtacttttta
     2941 tagactatta tcaccattcg atgatgataa tcaaaccgcg tggatggttg ttagtcatga
     3001 taagaaaacg gctattgttg ctcattataa aacattaaat gaggtaaatg ctccttaccg
     3061 ccgactgaaa ttaaaagggt tagataatga cggactttat aatgtaggac aaaatagttt
     3121 gcgatctgga atggaattga tgagagcagg actagtctgt tcagatgcat ctagtggaca
     3181 aataaatgat cagaatcgac gcccagaaac atttgatttt gattccaaga tttgggttat
     3241 aacacaggag gaaaagtaga gatgaataac caaaaaagct tacttcaaaa aggagcaatc
     3301 ttaagtattt cactaatgtt aacaagctct caagcaatta atggtgttat tccacaaatg
     3361 aaacagtctt tggggatatc acaaagtcaa agtgaacttt taggaactgc accaagtata
     3421 actgttattt tgtttatctt attatctagt tactttgcca aaagaatagg aatgaaaaag
     3481 acaataattt taggtttatt attagctggt ataggaggaa ttattccagt atttacagct
     3541 accttccaaa ccgttcttat tagtcgagta atattaggtg ccggtttagg agtatataat
     3601 tcattggctg tgacatatat cagctcttta tactctggca atactagagc aacgctatta
     3661 ggtattagaa attcaatgga agctttaggt cagacaatcc ttatttttct tgctggagtt
     3721 ttagtgaaca ttaattggac ggcttcgttt ttagtctatg caattgcgtt tcctgtagct
     3781 ctcttattcg cgctaaaagt tcctgaaatt agagatgaaa atagtgacat acctgaaaat
     3841 catgtaaaag aaaagatgaa tccaatggtt tttgccctag ttctatttgc aatcttactc
     3901 gtaatgaata gtattgctat ttctgtacga tttgcatcaa ttgcaactga aattaaaggg
     3961 gtaaacttta atgctagtaa ctatttagct ctaatgccta tcttaggaat actagcagga
     4021 tttatgtttg gaccaataaa taagtggata ggaaaaggaa ccctatactt gggaattata
     4081 ttctttataa tttcaaattt gttaattgct gtgtcaaacg gtaatatgac gttcctgcta
     4141 acgggtttat ttttatcaag tataacagga tcatggtgct tcccatttat ctttagtaac
     4201 ttagataaag tcactacaaa gaatacaatt aattttgcta cttcattgat ttttattggc
     4261 tgtaatattg gtaattttat agctcctatt gctatgcaat tagcacaatt tctaacaaga
     4321 agtacaaatt tgactgctcc attttttgta ttcgcaggaa tatttattgt gatcctattt
     4381 gtaacatttt ttgcgacaaa taaaaaaaat actagagtaa cccgtggtgc tagttaattt
     4441 taatatccta aagaatatct caaagttcct tcaaaacgtc gttgtgctcg acttaataga
     4501 acactgtaag ccagtaatat gctttcaaat ctactctcta gccctttaac agaacgtgag
     4561 ttgaaatttt gacaacctaa tttttctaag gaagaaaaaa cagtctcaac tgtttttctt
     4621 tgttttccaa gtaagctgtg gtctagtttt tctgatttat ccatgttttt a
//
LOCUS       DACDUU010000128         4641 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.128, whole genome shotgun sequence.
ACCESSION   DACDUU010000128 DACDUU010000000
VERSION     DACDUU010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.128"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IWL30_002771"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IWL30_002771"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP9707470.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="IWL30_002772"
     CDS             complement(2260..2511)
                     /locus_tag="IWL30_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAP9707471.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="IWL30_002773"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="IWL30_002773"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAP9707472.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="IWL30_002774"
     CDS             3461..3679
                     /locus_tag="IWL30_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707473.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3968..4480)
                     /locus_tag="IWL30_002775"
     CDS             complement(3968..4480)
                     /locus_tag="IWL30_002775"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP9707474.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4494..>4641)
                     /locus_tag="IWL30_002776"
     CDS             complement(4494..>4641)
                     /locus_tag="IWL30_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707475.1"
                     /translation="NGVLQKVASPESHPLTESRLYQRRLNKIKVEEFLLLSDIPTIKK
                     WLTT"
BASE COUNT         1279 a          996 c          851 g         1515 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa
     3721 cttttacggg ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta
     3781 aagccagttt ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg
     3841 gtctttgctt gtttccattt ataaaaaaat agcgaccctc aaaagtcaga tagagaatct
     3901 gacttttgag ggtcgcttta aaatcgcttc tttaagaata acatcaagaa tagcttcttt
     3961 tttctgctta atattccatt aatgtaatga tgtcatatcc tttgattttg tcgcgtccat
     4021 ggagatccat caattcgatc aaaaatgcgc agcctacgac tacaccgcct aattgttcga
     4081 tcagttcgat cgtcgctttg attgttcctc cagtagctag caaatcgtca caaatcagca
     4141 cacgttgacc tggtttaatg gcatccttat gcaatgtcaa tgtatctgtt ccatattcta
     4201 atccataagt cacttcgatg gtttctcgag gtaacttccc ttttttgcgg actggggcaa
     4261 aaccgactcc taactcataa gcaacaggac atcctacgat gaatccacgg gcttctggcc
     4321 caactaccat atcaattcgt ttttcttttg cataatttac gatttgttta gtagcttccc
     4381 gataagcgtc accatctgcc atcagtgggg aaatatctcg aaacacaatt ccttttgacg
     4441 gatagtcggg aatactggca atataatctt ttaaatccac tctttgtttc ctcctaggtt
     4501 gttaaccatt tctttatggt aggaatatcg ctcaaaagca aaaattcctc aactttgatt
     4561 ttattgagcc gtcgctggta taatctgctc tcagtcaaag gatgactttc tgggctagcg
     4621 actttttgta aaacaccgtt c
//
LOCUS       DACDUU010000129         4434 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.129, whole genome shotgun sequence.
ACCESSION   DACDUU010000129 DACDUU010000000
VERSION     DACDUU010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.129"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(543..2048)
                     /locus_tag="IWL30_002777"
     CDS             complement(543..2048)
                     /locus_tag="IWL30_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9707476.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDN
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="IWL30_002778"
     CDS             complement(2045..2731)
                     /locus_tag="IWL30_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9707477.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IWL30_002779"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IWL30_002779"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAP9707478.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1240 a          919 c          729 g         1546 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgcat tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat attt
//
LOCUS       DACDUU010000130         4425 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.130, whole genome shotgun sequence.
ACCESSION   DACDUU010000130 DACDUU010000000
VERSION     DACDUU010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.130"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            690..1658
                     /locus_tag="IWL30_002780"
     CDS             690..1658
                     /locus_tag="IWL30_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9707479.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="IWL30_002781"
     CDS             1669..2484
                     /locus_tag="IWL30_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAP9707480.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="IWL30_002782"
     CDS             2552..3262
                     /locus_tag="IWL30_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9707481.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="IWL30_002783"
     CDS             3298..>4425
                     /locus_tag="IWL30_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAP9707482.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DACDUU010000131         4396 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.131, whole genome shotgun sequence.
ACCESSION   DACDUU010000131 DACDUU010000000
VERSION     DACDUU010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.131"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..93
                     /locus_tag="IWL30_002784"
     CDS             <1..93
                     /locus_tag="IWL30_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9707483.1"
                     /translation="RGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
     gene            complement(151..1371)
                     /locus_tag="IWL30_002785"
     CDS             complement(151..1371)
                     /locus_tag="IWL30_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP9707484.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1391..1996)
                     /locus_tag="IWL30_002786"
     CDS             complement(1391..1996)
                     /locus_tag="IWL30_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP9707485.1"
                     /translation="MKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELPLF
                     NKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVDKP
                     VMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEE
                     QVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(2045..2977)
                     /locus_tag="IWL30_002787"
     CDS             complement(2045..2977)
                     /locus_tag="IWL30_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP9707486.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3398..4258)
                     /locus_tag="IWL30_002788"
     CDS             complement(3398..4258)
                     /locus_tag="IWL30_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9707487.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1257 a          882 c          727 g         1530 t
ORIGIN      
        1 cgaggattat ataagaaaac ccgaaaagaa ggcactctct tcgggttttc ggtctgtact
       61 gaaatcaagg tattattggg aatcccagct taaatcatag ataccgtaag ggactttatt
      121 ctttatttaa aactttgcaa cagaaccttt ttattttaca gaagcactcg aaactgcatc
      181 tactgcagtt tcatcgtgtg tgctggcgct ggttactgca tctgtttttc caccatgaac
      241 agatgcactt gaaaccgcat cttcatttcc aacaagttgt tgacccgttt gttgtaaata
      301 ccactcaatg attggtttga aatcaagaat gtattcattg attccagcat actctccatt
      361 ttcatcatgc attgcttggt aattatgcac aatatatttt tctggcactt gttttggtac
      421 atgaacacgt accgcatctg ttgctccgct tcgcagttgt tgaattaccc actctacatt
      481 tttatacgat ttttctgggt gacagttggc taatggattc cctacctgac cgggcacacg
      541 agcagctaac atctctttcg cttcttggcg caaattataa tataaaaatt ggttattatt
      601 atctgcaaaa gttaattcca tcggcatcga atctaaaaag taattgattt gatccactgt
      661 caataaccca cgatttaatt tgacgtaagt atttccagaa acggcaccga ctgcttcaga
      721 agctttttcc aaccaatgat catctgacat atcaacatcg ggtattgtcg tagcaattgt
      781 tcctgtcgca tgcaaatctt ctggtggaag ctttttaggc cctgctaagg tagacacgac
      841 tttaatgaat tttacaaaac ttgctaatgt actgcctaaa aagtcaacag ttcgtggatc
      901 aatcaagcga tcttcttggt caaatgcttc ttttgctttt cccaataaaa attcatttcc
      961 gggcatcact aatgcattca cgcctggtgc gtccaaaatt tggcgcaagt gtaattgcgc
     1021 gcgtgaagtt ccttgatcgt agtaagaagc acccacaacc attacaggtt tattttcaag
     1081 tggatggatt ttaaatgata gccattcaag tgcacttttc aatcctgctg gaaccgtatg
     1141 attgtgttct ggcgtagcaa taattacccc atcagccatt aagattttgc gatttagctg
     1201 ttgaatagca ggactatttg tttgatcatc atcttgattg aacagaggta aatctttgat
     1261 ttcaatgatt tctaaattaa atagttcatg atattgtttt tggataaatg ttaataattt
     1321 acggttatat gaaagttcag ctgctgatcc gacgatacct attaatttca tctttgtttg
     1381 ctcccctcga ttaaatcgtt tcccatgaaa agctttcggc ttcacgttta tttgcttggt
     1441 gcgcatgttg caattgacta gtgattgtga caaagagtaa gaaatctttg aatagttcac
     1501 gtaattttgc gacttgttct tcatcaatga gtcgtccttg ctcatcaaag gcttggagtg
     1561 aatgacccaa taaaaattct gagcttggca taattcgtgc ctttaactct ggtgaatcca
     1621 atatttggcg caaatgtgct tgtgcgcgtg aagaacctaa cgtgccataa gacgctccgg
     1681 taatcatcac tggtttatcc acaaacggaa aaataccata tgaaagccaa tttaatgcgt
     1741 taatcaatga agcaggaacc gcatggtcat actctggtgt gctgatgatt acgccatctg
     1801 cttgttcaat ttttccagca atttctttaa caacatttgg tagttctgtg ttttcaggtt
     1861 tgttaaatag tggcagctcc ttgatttcga caatttcgat ttctgcctca tttacaaaat
     1921 aatttttcat aaactgtaat aattcacggt ttgttgaccg cgttgaattt gttcctacta
     1981 atccaataaa tttcatcttc attctctttc ctttctaaca tcgatttctg actgttcgca
     2041 cgtactaaat tttttcaaat ccttttgtat acaagaattg attctctttt gttacaactg
     2101 ctccctcgat acccggctgt tcttcaagcg ctttcaacac ttctaaagaa gatgctccaa
     2161 ataaacgtgt agtccagatt tctccatcta cagaagaatc agacaaaatc gtcaggcttg
     2221 ctacgtcatt tttaactgga aagccagttt tcggatcaat gatgtgatga tacactgacg
     2281 aaccattttg tagataacgt tcgtagattc cagaggtaac taaagattgg ttgtttagtt
     2341 taaccaccgc aagcgactct tcacgtgaac ctttaggatt tcgcaaacca atacgccaag
     2401 caccatccga acgtcttggt gattcaccaa aaacaacgat atttcctcct aagttaacta
     2461 aagcaaacgc tgcatttcgc tctcggcaat gtgtaattaa tttatctgca atgtaacctt
     2521 ttgctaacgc ccccaagtca atcatcattc ctggtttggt taaaaaaacg gttttcattt
     2581 tttcatctaa ttgaatttgg tgtggatccg ttagcagaag taactcagtt atctctgctt
     2641 ttgttggaac caatgcatct tcaaatccaa tgtgccattt ttgtacaacc ggaccaatag
     2701 caatattcag ataactattt cttgcacaac tatgtgcttt tcctaatttg atgagctcaa
     2761 aaagttcagg atgtacaatc acagcttgtc gtccagcagc ttgattgatg gccattaatt
     2821 cagaagttgc atcatgtgcg ctgaagcgat gttcataaac gtgcaaccat tcgcctaact
     2881 ctgctagtaa tcgactaggc tcttcatgcc caacttgtaa tgtaattgtt gtccccatca
     2941 tttgccagat tttcgtttct tcgatcacct gattcatttt gtaccttctt tacttgaact
     3001 acggattaca tgagtaatta gtttttattt ttgttactgt tttcacaacc agatgatacg
     3061 cctgctagat ttatttgtca accgatttta tatatttttt tatataataa ggtaatttta
     3121 atataaaatt ttatattgta aaaaaacaaa taagggaaac aaacaacaca tttttgtgtg
     3181 ttgcttattt ccctctaact atcgttttct atttgatggt taacctactt aaaataaata
     3241 ctgcaacgac aaacggagat caagcatgct ttgtaacgta ttttacgata attacgactt
     3301 tattttatta ctctatcttg ctgccatacc cgtctatccc taatcatctt tggtgaattt
     3361 tttcaaagtt agcataacaa aaataagtat tttttcctta gttaatcaat agtatcattt
     3421 ttttcatatg atttaattca tcaattaatt ttttgttttt attaatttta gcaaaagcta
     3481 gtgcatcttg aatcaattcg agcacttctg ccttcgactt atttttagcc tgtgcgttct
     3541 tcgctaattg ggcagcagct cgtgctaagt aataagtcaa atggttatcc gaacaaatat
     3601 cgattgcgta atttagtagt gcatcacttg tttccaaatc accgtcttgc gcaaaaaaaa
     3661 caccacaatg aaagacgata tttaacaccc gccagatatc ttctgttgta cgaatcggat
     3721 acgtataaat tttttcaaac actcgattga aataaacttc tgctttttcc aactcttttt
     3781 ctctagcata gaccattcca atacctgtat atgccaataa acgaaacaac gtgttggctg
     3841 tttctgccat caacaactga tcaaaatgga acaacacatc cgtaattggt gcattcgtaa
     3901 aaatcatttc aaatcctttt agatagtagt aacgtgcaat cagctcagac tcgtcaatta
     3961 ccttgggatc aatggctgct aaaatctgtg tcaattgttc atattcactc gtaatcagtg
     4021 aaaactctgc tttttctagc tgttcatcaa gttgtgtatt gatcacgttc atcgaagaaa
     4081 aaagttcaac taacggaaga ttgattcgat tacataattt gattaaaatt tttaacgaag
     4141 ggacttgacc attgttctca aatttactta atgttgcttg tgtacaaatt ccttcagaca
     4201 cttctttttg agataagttg gcagcctttc ttgccgtaat aaatttttca atgttcatta
     4261 ttttctctcc aatcagacct cacacgaaaa tcatttctgt ctcgatttta gcatgttttc
     4321 tttgggaatg ctttaggttc tgttgcaaag ttttaaataa agaataaagt cccttacggt
     4381 atctatgatt taagct
//
LOCUS       DACDUU010000132         4158 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.132, whole genome shotgun sequence.
ACCESSION   DACDUU010000132 DACDUU010000000
VERSION     DACDUU010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.132"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            180..776
                     /locus_tag="IWL30_002789"
     CDS             180..776
                     /locus_tag="IWL30_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP9707488.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            900..2699
                     /locus_tag="IWL30_002790"
     CDS             900..2699
                     /locus_tag="IWL30_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP9707489.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2977..3189)
                     /locus_tag="IWL30_002791"
     CDS             complement(2977..3189)
                     /locus_tag="IWL30_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707490.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(4053..>4158)
                     /locus_tag="IWL30_002792"
     CDS             complement(4053..>4158)
                     /locus_tag="IWL30_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9707491.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT         1228 a          707 c          729 g         1494 t
ORIGIN      
        1 ttttaaaaaa aacaagttat taaataagtc tttagttgga tgtttttttg agatcaatct
       61 tttatactta tgatgaactt acaaaattgt ttgttttctg tcatatcaaa aaatggcgca
      121 gttagttgct tttctagtaa agtatagtta acaaagaacc tctaaggagt tgatgaataa
      181 tgaatattgc tgcctctcga aaactgataa atttcatatc cattatcgga ttaggcctat
      241 caatcgcgct gacgatttat tttattaatt taggtgtttt caaagatttg aatgcgttaa
      301 gaggattagt aggtgattcc atcattttag gaccgattat ttttgtcttc atccaaatcc
      361 tgcaagtagt tattccaatc attcctggag ggatcagtac tgcagctggt gtcctgatat
      421 ttggccctta tgctggattt atctataact atattggaat ctgcattggt tcgattatca
      481 tttttctgct agggcgtcgt tatggaaagc cattcatact ttccatgatt agcgataaga
      541 catataacaa atatattggc tggctggata atcaaaaccg ttttgagaag ctgtttgctc
      601 tagctatttt tcttccagtt gctcctgacg atgcgttatg tttgatggct ggattgacaa
      661 acatgtcagt gaaacgatat acactaatca ttttgattgc taaaccactg tcgattttct
      721 tatatagtat ggccttgatt tatggaggtc aatatctaag tggtcttctt ggataatatt
      781 tattttgtaa aattagtcat tgattgacaa tgttaaatct gggaaagggt ctgcaaaaca
      841 cactgcaccc tttccttttt cattagattt gcgctacaat gtgaagagca ggtgatagga
      901 tgaattattt gaaacgtagc ttaggtaatg cttgggactt tttaaaagga acacaagctt
      961 atttccgtga tgtcttgctg atgcatggct ttattttatt tatttgttta cccttattga
     1021 gcagtacgac ccgttttatt ttacaccgag gggcaattga ctatttgtct tatgacaata
     1081 ttccttcaat tttttctcaa catcctgggg ttctactctc gttaatcgta gttttgatga
     1141 cgattttact gttagtttat ttcgaattta cttttctttt gatgagtgtt ttttttatta
     1201 aaaagaaaga accaatctct ttgaagcaac tcttacactt gacgatactt cagctgaaga
     1261 aagtgagacc cattactttt ttgtttttcc ttgcttattt tttattgatt ctaccgatca
     1321 gcggattaag ttttaattct gatctactgt caaaaataaa aatccccgct ttcattatgg
     1381 attttatttt tgctaatcgt tggatcatcg tcagttcctt tttactcgtt tatattttcc
     1441 ttggctatat tgggattcga ctgatttttg ctttaccgga aatgattcta cgggatcgtc
     1501 cttttcgagc agccattcgg gaaagctggt cattgaccaa atcgcgtctt ttggccatca
     1561 cgggccaatt tattgtgatt ggcggtacga ttttactcct ttcttcttta ggatatatcg
     1621 ttgttatttt ggcacaaagt atggttgaac aatttttccc agactatgca ttgatcagcg
     1681 ctgtgtttgc tatgacgctt ttacaaggga ttttattatt caatatcgtg atgtctaccg
     1741 tagggatttt ttatatcatt gttgatttca tggatgacga aggcttcttg cctgagatcc
     1801 ctaagtggtt tatcccacaa gcccccaact tgcgtttctc tgcgctgaaa aacactggtt
     1861 tgactctttt tgcagttttt ttcggtattg gcgtctgtct ttacaatatg gattatctga
     1921 cttcagctgt tcagacaaaa ccagtgactg tctctcacag aggtgtgagt acccaaaatg
     1981 gggcgcaaaa tactttagaa gcacttgaaa aaacaagcag agactatcat cctgattatg
     2041 tagagatgga tgttcaggaa acaaaagatg gtcatttcgt agtgatgcat gatgcaaatc
     2101 ttcgtcactt aacaggggta aatggaacac cacaggatct tacattgaaa gaattgacaa
     2161 atcttacagt tactgaaaac ggagtgcaag cccctgtttg ttcttttgat tcttatttgg
     2221 aaaaagcaca taagctaaac caaaaattac ttattgagat caagacctct aaaaaagatt
     2281 cggatgacct tgtcgaacga tttgtccata aataccgcaa agttatctta gagaaccatc
     2341 atattttgca aagtttgacg taccaaaccg tttcagattt aaaagaagca gaaccaaact
     2401 tttatgtggg ctacatctta cctttcaata ttgttgggcc ccctgtgacg cctgctgatt
     2461 ttctgacaat ggaatatagt acgatcaacc gtaacttcat taattctgcc caccaagacg
     2521 gaaaagaagt atatgtatgg acaatcaacg attcagatgc tatgagtcgc atgatgtttt
     2581 atggtgtgga tggtatcata accgatgaga tgggactact aaatgaaagt gtgaaacaaa
     2641 cagatgaaga aatcacttat tcagataaac tgctaaattt tgttttggga gtcggctaat
     2701 caaaaatcgt taatgaaaaa agtctttata caacaaaaaa cgcataacct gccaccgcag
     2761 gtatatgcgt tttttgtttg acgctcataa tgtgtgctta actttctctt gattccttca
     2821 tcgcacgaac aatagctaaa atagcaggaa ttgcagagat cactgcaaga ataattccca
     2881 ttttccctga actttttctt tttggcatat cttctcgctc ccttctgtct ttatttatat
     2941 tataagggag ttcttgcgaa ttctcaaaaa tactgtttaa gaaaccaatt cagcaggtgc
     3001 gctaatatca cagaatttta cgagcatgcg atacttgctt tcattgataa gttgatagtc
     3061 tcgttggtca taactttcaa catacacgac agccgtgttg atatatgtac gttcaataca
     3121 gccgattagt tcatgagata ctccgatcgg tttgcatata taagatcttc ctacttctgc
     3181 ttttaccatt tcattccctc ttttctttat ttttattttg ttttttcttt attttataat
     3241 aacaaattac cttgtatttg aaaacgtttt tatccatttt tttatttttt attttgagta
     3301 tctttctgct ttttagataa ctatttttta acacgatctt agacgttgtg acaaaaacgt
     3361 ttgtttatat tcctatgttg ttcgtgcgtt tttttcttta tattgctaag ttatttataa
     3421 agaggtgagc atatgattta tattcaagca atagtatttg taaccttgat gattgggcta
     3481 cttatcggat taaaagaaaa tagctaaacc atttaagaaa aaataaataa ggaaaatgat
     3541 aaatgctttt taattgcatc aaaaaacgta cgatgctaaa cttggtataa gttcgaaagt
     3601 ttaaaggaaa ctttcaagaa ctaaattaca ttacaaagga gatgaatttt atgacgttta
     3661 caaaaacaaa tatgcaatac cgcgtagctt ttgatacaac ttctaaccgt tttatggcga
     3721 ttgatgcaca aaacgaaaat aacgttgcgt ttggtattac aatcgaacaa gcagtccgag
     3781 cattaaacga atctagataa ggagtgaacc attccaaatg gcgactgatg agatctcgga
     3841 aaatcaataa aagacgtttg taacggtaat ccgcgacaaa cgtctttttg tattcattac
     3901 atatatttgg aaattgtctc tctttatctc tagacctaaa caaaaacaca agttctaata
     3961 tcaaccatct cgttttttat gacaattttg ttagctgttt cgatgataat attccgttat
     4021 tatctgtata atatttaata taatcttaat tgtcaaatta agttagacag atcatcaata
     4081 cttacgtaac tcaaaaatac ttccaaaggt gttcgataat ccagtgattt tctaggaata
     4141 ttatttcgtt tagatgcg
//
LOCUS       DACDUU010000133         4151 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.133, whole genome shotgun sequence.
ACCESSION   DACDUU010000133 DACDUU010000000
VERSION     DACDUU010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.133"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..547
                     /locus_tag="IWL30_002793"
     CDS             <1..547
                     /locus_tag="IWL30_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP9707492.1"
                     /translation="CDIVDIPDDFVSQYRVVDPVSFKPIYGRSGKLETAYWGSRASER
                     QIRMYNKKLEQERKRKIVPPEITTWWRLELQLRRGKATDWHEMVHESLDSFASPHYFP
                     PNTKVTERIMVKGLLYDHSEWGNLAKNTKKKYRDLLKQESQNDELTNHLRESFAESAD
                     DLKRELDTWLQGLDVTEVGAE"
     gene            544..963
                     /locus_tag="IWL30_002794"
     CDS             544..963
                     /locus_tag="IWL30_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707493.1"
                     /translation="MSNPVEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            972..1280
                     /locus_tag="IWL30_002795"
     CDS             972..1280
                     /locus_tag="IWL30_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707494.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERIMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            1284..1646
                     /locus_tag="IWL30_002796"
     CDS             1284..1646
                     /locus_tag="IWL30_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707495.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            1867..3273
                     /locus_tag="IWL30_002797"
     CDS             1867..3273
                     /locus_tag="IWL30_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAP9707496.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDEN
                     IYLEQTNIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     TLDDFRTTRDDGSSTGISEQQAEQNRVIANKILVEYFKEFKERNPNLSVYNAVIHNDE
                     VSPHLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLRADKENGRVIWNNFRNQEVESIE
                     RLMADFGWERELVGTNKIKDVHEYKEIMSEISELRQTAVKERESVSDELRYISRQREE
                     NEREKALVASERVKIEEEKKNMLNVKKEMAVIFQKDIVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQRKLERSEDEIEW"
     gene            3720..>4151
                     /locus_tag="IWL30_002798"
     CDS             3720..>4151
                     /locus_tag="IWL30_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP9707497.1"
                     /translation="MSKRGTIVNEWEKLNRISNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYLKAVGNNGWQLLDQHEENIAYIEILKF
                     HEGKGRIDFNPNKINQFLSGSMKNFIHDLFLEPHFSRADIAC"
BASE COUNT         1444 a          603 c          930 g         1174 t
ORIGIN      
        1 ttgcgatatt gtagatattc ccgatgactt tgttagtcag tatcgtgtag ttgatcctgt
       61 gtcttttaaa ccaatttatg ggcgttctgg taagttagaa acggcatatt ggggtagtcg
      121 tgccagtgaa cgacaaattc gaatgtacaa taaaaaattg gaacaagaaa gaaaacgtaa
      181 aattgttccg ccggaaataa cgacatggtg gcgactagaa ttgcagttac gaaggggaaa
      241 agcaactgat tggcatgaga tggttcatga aagtttggat agctttgcta gtccacatta
      301 ttttccgcca aatactaaag ttactgaacg gattatggtg aaaggtttgc tttatgatca
      361 ttctgagtgg ggaaatctag ctaagaatac aaagaaaaaa tacagggatt tgttgaagca
      421 agaaagtcaa aatgatgaat tgacgaatca tttgcgagag tcatttgcgg aatcggcaga
      481 cgatttgaaa cgagaattag atacatggct ccaaggactt gatgtaacgg aagtaggtgc
      541 tgaatgagta atccagtaga aaaaatgacc ataaaaaatc ttgctgacga attgggtgta
      601 tcgaaaaata cagtgcgcaa tcaactggtc aattcagggt ttgaccttgc tcagtataaa
      661 gaaaatggca ttatcatgct agataaagag ttgattggtg ttgttcggtc aatttatcgg
      721 tcaaaaagtg atcaattgac tggtcaatta ccggtcaata acacaggcag tgattttcct
      781 ttagaaaaag agaatgagtt tttgcgtgat caattagctg agaaggacaa acatatttct
      841 gaattaacag aaatgattcg tgatcaacaa aaattgaccc tgcttcagca acaaaaaaac
      901 gatcagttgt tactagaaaa tcaagaatta aaaaatcgaa aatggtggca attctggaag
      961 tgaggtgtga catggataaa attgatttga gagaagaaac agtcgataga attgaattga
     1021 tgtatgaacg gttagctgat tatttgaatt tgccggatga agataattat agtgaaatgc
     1081 ttgaacgaat tatggatgtt ctcaactcgg ttatcaatgc tgatttagca aaatcttcag
     1141 ttttgactaa tgaatttgtt cataagcatt tagcgcaaac aactgatgaa tcagaaaaag
     1201 aattttggtt gaaattaatg agtcatgtta tgcagttaaa ccaaatttcc gttattaatt
     1261 ccaaagaaaa ggagatgtag tttatgggaa tgactttagc agatattaaa gcgcaagaag
     1321 aaaaattaaa aaaagaacaa gcgaaacttc gccaacagaa aaaacgcttg attgagaaag
     1381 aaaatgctgc aatgggcaag gctcttcgca agcgttttgg gtttgaaact tttgctgatt
     1441 ttgagcggtg gctatcgtcg atttctgacg atcagcaaca cgaaaaatca gtggataatg
     1501 agtggcttgc tgatgaaaaa tcagcgccaa gcgaacgaga ttatcccgat gaatctgacg
     1561 gcttattcgg agtgataccg aataagaaaa gtcagatgag tgagagtaaa tttacgtcaa
     1621 tatttaatga cgtgaattta cgataagcga ttattttgcg tgaagcaaaa taaagcgtaa
     1681 aaggagttga gagcgtgaca cgctttcggc tccttttttc gttgtcactt gtgacagcga
     1741 ataccctcgg agagcatttg gaattgtgac agccatgtca caattaggaa aatgttactc
     1801 tttgacaaag agtaacagaa aggtttacac tctttttatt agcgacagcc gaaggagggt
     1861 tttaacttgg ggttcagtat ttcgttcaaa aagggaacaa agaatacaag catttctcat
     1921 aataatcgtg agttaactga tagccaaaaa atcaatgact ggcataagca tattgatttc
     1981 acaaaatcgg atgaaaatat ctatttagaa caaacaaata ttcgtgaaaa atatgaagaa
     2041 ttattcggtg aagccgtaga aaaatataat gcaaagcaga aaagagccga tagaaaaatc
     2101 gaggactatt tagcaaaggt tcgcaaggat aaaaaattgg aaccacagcg agaatttatc
     2161 gtgcaaattg gtactcttga tgattttcgt actactcgtg atgatggttc ttctactggc
     2221 atatcggaac aacaagcaga acaaaatcga gtgattgcca acaaaatttt agttgagtat
     2281 ttcaaagaat ttaaagaacg gaatcctaat ttatccgtct acaatgctgt tattcataat
     2341 gacgaagtaa gtccgcacct gcatttgaat attgttcctg ttgctgaagg gtataaacgg
     2401 ggtgttcaaa agcaaccaag ttttaacaaa gccttgcaac agcagggttt acgagcagat
     2461 aaagagaatg gtcgagtgat ttggaataat tttaggaatc aggaagttga gtcaattgaa
     2521 cgtttgatgg cagattttgg ttgggagcgt gagttagtcg gtactaacaa aatcaaagat
     2581 gttcacgaat ataaagaaat catgagcgag atttcagaat tgcgtcaaac ggccgtaaaa
     2641 gagcgtgaga gcgtttctga tgagttgagg tatatttcta ggcaaagaga agaaaatgag
     2701 cgagaaaagg ctctggtggc ttctgagaga gtgaagattg aagaagagaa gaaaaatatg
     2761 ctgaatgtaa aaaaagagat ggcggtcatt ttccaaaaag atattgttcc tttagaaagt
     2821 ttcaagaggc ttcctgatgg ttcgtatcat ttatctggga atgattttgt tgatttgtat
     2881 caacgagcta cgagagcaac aaatgccaag agaaatgctg ataacatgag tgagcaatat
     2941 ttacagatgc ttgagcaaaa tacagaatta aatgagtatg tagctacatt ggaagaagag
     3001 cgtcatttgt ctggtagaaa acaattagca gatttacaag tggaaaataa aaagctgaaa
     3061 gaagaaaatc gctctttacg ccagcgttta gagcgattaa aaaacgcatt tcaaaaatcg
     3121 atcactcgtt tgtctatccg ttttggtctt gcagaaaagg atatggggct ttctgaggaa
     3181 ttagaagtgt tagagcagga gaaaacctta ttttatcgtc aaaacgtctc acagcgtaaa
     3241 ttagagcgtt ctgaagatga gatcgagtgg tagttacgat gtgcagtcaa cggtaccgga
     3301 ggcggtttgt gccgaggata aggagttgac ggctaaacca ctagcctatt aagctgacat
     3361 ttgctaaaaa atggccagtc cattttttgg ccaacctagc gttagcgaag tttgggggcg
     3421 cccttttcaa agggtgcggt tttatttttt ggatatttgc atagctaaag agaagtgcta
     3481 ttattgagtt agcaaacaaa tgaactgaaa aaagagtaaa aaaatagtcg agcaacgcta
     3541 ccaacgttct cgacctttac ggaaactaat cgcactagct ccgtaacttc ttaatcatta
     3601 tacgaatatt tttagatttc ttcaagacta gagatttttt ggtaatgaat ttttaagagt
     3661 tacgtgaact tttgaaaatt ctttccagaa gtctcttttt ctttgaggag gaacaaaatt
     3721 tgagtaaacg tggcacgatc gtgaatgagt gggaaaaact taaccgcatt tctaacaggc
     3781 ggttaaagta tcaaagtttg acccttccct taaaaatgca gtggtcaatt gaccgaatta
     3841 ccatagtcgg aaaactgaaa gaaaatattt attatcacac tccaaatgat gtgttgattt
     3901 taaattttga acagcttatg cgtttgaacg aaggcaatgg ttatttaaaa gctgttggga
     3961 ataatgggtg gcaattatta gaccaacacg aggaaaatat agcttatatt gagattctta
     4021 aatttcatga aggaaaaggg cggattgact ttaatcctaa taaaatcaat caatttttat
     4081 caggttccat gaaaaatttc attcatgatt tatttctaga acctcatttt tcacgtgcgg
     4141 atattgcttg c
//
LOCUS       DACDUU010000134         4092 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.134, whole genome shotgun sequence.
ACCESSION   DACDUU010000134 DACDUU010000000
VERSION     DACDUU010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.134"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..153)
                     /locus_tag="IWL30_002799"
     CDS             complement(<1..153)
                     /locus_tag="IWL30_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9707498.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
     gene            complement(475..696)
                     /locus_tag="IWL30_002800"
     CDS             complement(475..696)
                     /locus_tag="IWL30_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707499.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(794..1039)
                     /locus_tag="IWL30_002801"
     CDS             complement(794..1039)
                     /locus_tag="IWL30_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707500.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1279..1893
                     /locus_tag="IWL30_002802"
                     /pseudo
     CDS             1279..1893
                     /locus_tag="IWL30_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2263..3264)
                     /locus_tag="IWL30_002803"
     CDS             complement(2263..3264)
                     /locus_tag="IWL30_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HAP9707501.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(3423..3689)
                     /locus_tag="IWL30_002804"
     CDS             complement(3423..3689)
                     /locus_tag="IWL30_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9707502.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(3679..4035)
                     /locus_tag="IWL30_002805"
     CDS             complement(3679..4035)
                     /locus_tag="IWL30_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP9707503.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1295 a          744 c          542 g         1511 t
ORIGIN      
        1 tacccaacga taaatcgtag tatgacaaac atttattcca cgatcatata acaattcctg
       61 aacttcacga tagcttagat tgtaacgcag gtagtaacca acagcgacaa taatgacgtc
      121 ttttttgaat tgtttgcctt taaaatgatt cattactctg tcctctctgt cttttttctc
      181 aattttacac taaaatagat tttttggaaa actttgcaac agaaccgttt ttactatata
      241 cttcttccca gaacttgtcc gtctccttca aattatctct acctctcgag ttcattgggt
      301 ggtttgccca tttattaaac atggaatcac ctttctcata ctcatatatt tatgtcctat
      361 ttcaattata tttatatatg cttaaataaa tttgtatcct gaaatttatt gtaatatgaa
      421 gctgtatttt acatatagta accaaagagt taataaaata aaaagagcga accattatag
      481 atcagctctt ttttcattgc tgtttttaat cttatctaga atcacttcaa atgtttcaaa
      541 ggtatttaca tctaactgac ataaccaaag caagtacttc ttttctttgt aagtccaatc
      601 aatattacgg taagaggaca ttagattatg gtaccttgct ctttgttcaa ctgaagccaa
      661 ttcccagcgt ttttcaaata ttttttcttc ttccattatt gatccctcgc aagacgctac
      721 ctcaaactta aataatgggc agcgaaagtc taattcctta gatctaagta tagcaaacaa
      781 atatgaaaaa aacttagaag gtagtaaaag ttcttccgtc ttttgctttg cttctattct
      841 taatgtgttt cgttatcggt gccctacaaa gattttcatt aattacacac tcatttattg
      901 ttgtgtattt tctgacattt ctctcttgat caattatttt aattccatgg gcatcctgat
      961 agttcatagg agaggagcgg ctatcagaaa tagttgcacc tatctcttga acttctttta
     1021 caacttctgg aaaatccatc catcctgctt cagctgtcaa aagtaataat ctataatatt
     1081 tatctttcct tttcaatcta agaaatcgtc accccattct ttttcttcta ttataaacta
     1141 tttaactttt agaattcaaa aaccgtttgt ctatttagag tttaccctaa atagacaaaa
     1201 atataacatt gaaaaattta gagttctaac gtatgattag ggtgtattat aaacgttata
     1261 gaaaagtagg gaataaattt gaaaattgct tatattagag tttcatccat cgatcaaaat
     1321 gaacaacgtc aaattgaaga aatgaaaaag tttggcgctg aaaaaatttt tatcgaaaaa
     1381 caatctggag cgacgattag tcatcggccg attttccagg aagctttaga ttttgtcaga
     1441 gagcaagata tttttattgt agaagccatt gatcgacttg gacgcaatta tgacgagatt
     1501 atcgattcag tgaattatct aaagaagaag aatgtgcgac ttattatcac cagtttaccg
     1561 atcatggctg aagcaattgg aaatcctctc ctagataaat tcatcaaaga cttaatcatt
     1621 taaatctcag ccatgattgc tgaacaagaa cgtactgaat cgaaacgacg ccaggcacaa
     1681 ggaattaaaa tcgctaaagc aaacggcgtc tataaaggtc gtcctaagtt atacagtgca
     1741 gacaccaaag atcctcaacg tcgccttgtc tataggagta tcgttcagga tcttgagaat
     1801 ggggttgcta tctcaaagat tgcaacagac tacaatgtga ctaggcaaac aatttaccga
     1861 ataaaaaaag aaatcgacca actaattgta tgatttcttt tttgaatcac gtaattatta
     1921 attagctttg tcatcaatac agacaatcat tttagattca tctagctaag ttgattacat
     1981 ttatgttaaa aagaatttag ttaacataaa tataattgat gcataaatcg tccttattta
     2041 aaggcattta acattgacaa cttagtataa tgtatcttat gtaaactaat aagatttgaa
     2101 ttttatttta tacacactcg ataatagatc aattttatgg tcccccgcat ttacgattgc
     2161 acaagagaaa cataaagtcg acctattagc atttcttttc aattcaaaca ttaaaaaagt
     2221 ttatacctct gctactaaaa aatgaaatta tctatttaaa atttaaaacg gtaaatctga
     2281 ttcatccaaa ttggaaaatg gatcagcctg tattggagct ttgtatttct ccataataac
     2341 agatatcttt ttttcatttt ttaaaaaata ccttttaaat ttcttatagt cctctgaaat
     2401 caaaatatag cgtgatggat actcatcaaa agaagaatcg tattcttcct ttaaaattcc
     2461 cgatttactt aatttttcta gtacatcaaa tactctatca ctcaagtaac tccttaaatt
     2521 gttatcactt tcccattgaa ctacttttct ataagttgaa ttatcagttg aaaagataga
     2581 attttcatcc ataaacgcat atattagcag caatctttca tcattgtcaa attctattgg
     2641 atcaaaaata tctaagttgt catcaaaatc tttatcagtt ccggtaactt ttttgttaat
     2701 tactgaaggt gtcttgtaag aaatagaatc taaatcaaag cgttttgatt ctaaattatt
     2761 ttgtgctttc tcaattaaat aattcacttt ttttgtgaga aaccttctga tattgtcttt
     2821 gctatatgtt ccataaaaac ccttatcttc taaagagtcc ctgtacttct taacttctat
     2881 atactcttca gccaaaactt gtggaactgg ttcatctaag aaaaaaaccc ctacctcgcc
     2941 tttgctattt ttataaaagt aatcaatttc tgctatagtt cccgatctat aattatcaac
     3001 tggagaaccc aacttatggc caaaaatcgc taataatata tcactatttt ctacaatttg
     3061 tttattaata acactttgcc cagagctatt atttatataa gtaggaatcg cattcgtttc
     3121 ccatcgaatt ggcatcaaaa ttacttttgt attatctgta ttttctatat tccatctata
     3181 tatggtttct tctacttcat tcctctttgc tgctatatca cctggtgatg ctataaatac
     3241 ttttattaga tttatggttt tcatttattg tacctttctc tttatgtctc tttaaagtaa
     3301 tttttgtata aaatttattg acttttacac tataggacaa cttttatcta tttagtacaa
     3361 aaaggcccac taaactaatt gattagttag gagcctatta caggtatatt ttcaactcgc
     3421 ttttaagcaa attctactac cagctttttg cctaatgccc tagcgatatc atcaagttta
     3481 tcgaaagata cgttatcccc tctctcaatt cgagcaatag tagattgagt tgtatgtgct
     3541 ttttgtgcaa gctccgcttg agttaggcct gcttcctccc gtgcatgata aagagcaacc
     3601 gcagtttcga gttttcttcc ttcacggtca tatgcttctg caaaatcttt gtctttttgc
     3661 ttttcttcaa tcaatttttt aatcatcatt tgtgaaccac tcctctctaa gttcttttgc
     3721 atgtttaagt tcacttattg gtgttttctc cgttttttta gtaaatccat gggtaatgac
     3781 ataccttcca ttgaccacat ggaaatagat agctctttgg atgtttgatc ctaccttact
     3841 tcttaattcg tacaaattag tgtctaattt tttcacccat ttcaaccgtt gagctactag
     3901 catcccattc ttttctgttt cttctattgt acgtaaaagc ttcgcactat ctttttttgg
     3961 taatgtttcc aaccattcta aaaattcatc atgaccatta ggtcttttaa atgtttcaaa
     4021 agatattttt tccataccca attatatcat atattgattt tatattatag tatataacat
     4081 aaaaattcga at
//
LOCUS       DACDUU010000135         3808 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.135, whole genome shotgun sequence.
ACCESSION   DACDUU010000135 DACDUU010000000
VERSION     DACDUU010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.135"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            107..1248
                     /locus_tag="IWL30_002806"
     CDS             join(107..335,335..1248)
                     /locus_tag="IWL30_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9707504.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
     gene            1425..2324
                     /locus_tag="IWL30_002807"
                     /pseudo
     CDS             1425..2324
                     /locus_tag="IWL30_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
     gene            2404..2799
                     /locus_tag="IWL30_002808"
     CDS             2404..2799
                     /locus_tag="IWL30_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707505.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            3124..3741
                     /locus_tag="IWL30_002809"
     CDS             3124..3741
                     /locus_tag="IWL30_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9707506.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
BASE COUNT         1362 a          605 c          733 g         1108 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc gtagaatcta actactaaga aaaggacgtg tccacgatgg caaaagccaa
      121 gaaaaaattt gatgaagact ttaagaagat gatcttagat ttaaaccagt ctggccaatc
      181 ggtcgaagaa ctggcagcgc aatatggtat cgctacacaa acgatttatc gttggaagaa
      241 gcttcataca aagaatgtag caaccggcat gactgaggca gaaatcctag cgatgaaaaa
      301 ggaaatggcc cgcatgcagg aggaaaacac tatcctaaaa aaggctttaa ccatattcgc
      361 tcaaaagtaa aaatgagtga tgtcttcgaa tttattcaat caaatgttca tgatcatgac
      421 gtaaaacaaa tgtgtaccgt tttggaagca ccgaaatcca gctactatga ctggaagaaa
      481 aagaaacctt ccaagcgcca atcagaaaac aaacgcttaa accgcttgat ttcaggaatc
      541 tactttgaaa acaagggtat ctatggtgcg cctaaaattc ataagattct tgttggccgt
      601 ggcgagacct tgtccttaaa gaaagtacaa atactgatga gaaaattagg cttgcgttca
      661 gtcaccctca agaagtacaa gcctggcaaa caagcgaaag ttaaaagtga aggacgtaaa
      721 aacctgttaa accaagattt taccactacg tcgatcaatc aaaaatgggt gacagatatt
      781 acctatattc ataccctaaa agatggctgg acctacctct ccactattca agacctccac
      841 acaaagaaag tcatcggttg gaagtttggc aagcagatga cgaaagaatt ggttatcgaa
      901 acccttgaca atgcgctctt aaaccaaaca ccctccgaga acttgatcat ccactcagat
      961 ttgggttctc agtacactag cgaagcctat gaggcaaagc tgaatgaatt gaacattcgg
     1021 cactccttca gtcgtaaggg ctgtccctac gataatgccg gtatcgaatc gtttcacgcc
     1081 tcactcaaaa aagaagaagt ctatccatca aaagcgtatg aaaactttga agctgcttat
     1141 ttagcgttat ttcaatacat tgaaggattt tacaatcgca agcgaatcca tagtagtatc
     1201 aactatttaa caccaaatca gatggaggag ttggcattgc aagcttagtt cctagctacc
     1261 acttacattt ttttggcgag atttccacag ggtttcaact tcatcgcttc gatcagcggt
     1321 caaacggttt tggtttgacc gctggtcgaa acgatgagat actcacccgc tggaatctcg
     1381 aaaaaattct cacaaatcgt gtccgaggta ttgactcaaa tccagattta atcgctacat
     1441 cgggtagaga tagcggattt aatgagttat caaatgatac taataatgat aattacattg
     1501 ccaaatacaa tcagaaggtt ataaatcaga tggtacgtgc gtatcaatat ctatcacaag
     1561 ctggtcttga taatccttgg aaccctcatt tgacatttta tagatatgta aattattctg
     1621 gtttgtcatg ggacatcatt tcttctcctg taacagagca tgatagaaaa atttatgatg
     1681 aatataggaa taaattaact aatagcgagg gcctgtataa ttattttata gatcctaata
     1741 ctggttcaat aataggtttg cctcacttaa ttgtgacgtt acaatcacag atgtttctag
     1801 ccgacacaat caatgatagt gtttctgact ttgcaggttg gttaggagat ttaatgactt
     1861 gctggggtga agttaagaaa ttaggaatac aaatggagca aggtgtattt gcattagtag
     1921 gtacggctgc aggtggaaca tttagtctag aagatttata ccaagatgtt gacgcggtaa
     1981 atgtttatac cttagcaaaa agaaattctg attcaacagc tttatcggct ttaaaaacgt
     2041 actattgtga taagggtgct attgatagat ttaatcaatt tgttaagaac agatttggtt
     2101 cttcagataa aatcgcttca gtaacaaata gtttattaga aaatgacgaa acagttgata
     2161 tcttaggtgt agggataatt tcatttttca ttgataaaat agttaataat tcacttggga
     2221 cagattttac ttatcttgat tttattggag atgaggggaa agttgcaaat ggattttctg
     2281 aaaaaattat tcaatttcaa gaaaatagaa gtaaacttaa ttaagaatcg tttactattt
     2341 aaacagtgtc cttttccttt ttaaatatat acgataaaat aagtatatga attgtaaaag
     2401 gacatgatga ttatggaaga aatagaatgg aataatctga ctcaaaataa taaaaataat
     2461 tctggatgtg gaattatctt tatgtttgtt atagttttgg gaatagtagt acttttaata
     2521 actgtcaaga tagtagatta ctttgcgaat actcctgaag aacaaataca gagaaaactt
     2581 cctgagtttg aaagagtacc accttatgat ccaagagata attcatttca agtaaaaaaa
     2641 ttaaaagagt ctccaaatga aatgtggttt gaagttacag ttgaatcaag cgacttcaaa
     2701 gaaaaaaaga aagaatacaa ttatattgta aaggcaaaag tatcaaaaag atttattttt
     2761 aaccctaaat tagaaatata tagtgttgta atagaataat agtgttaaaa aaattgtaaa
     2821 agttaaagaa gtcaaattct gatgtgaaaa ataaaatata ccatggaaga cataaaagaa
     2881 aaactggata actagtttat ttaatggcag accgttaagg cctgccattt ttatgtattt
     2941 tagcggaata aatataatca tgcgggagtg taaacttact gtttcaaaaa gtatactttt
     3001 tgacatgcct tgattttctt tgtttatgta tggcataata accacataat acaatttgaa
     3061 acgtttctga tttaatctga ttgttttgaa acaattttag tgaaaaaagg gtagataaaa
     3121 acgatgagaa aattttttta tatccgggtc agtagttatg atcaatcgat cgaacgacaa
     3181 ctgatttccg cgaaggaatt aggaatccct gaagaatata tatttgtaga aaaagccagc
     3241 ggcaaagatt ttaaacgccc tgaatatcaa ttaatgaaac ggatgtttcg aaaaggagat
     3301 ttactctatc tacaatcatt agatagacta ggcagaaata aacaaatgat tttagaagaa
     3361 tggcgagaat taactaagga aaagcagatt gatatttctg tgttagacat gcctctgtta
     3421 gatactgcta gttacaagca actagatggg ttagagacgc ttatctctga cttaattctg
     3481 caattactca gctatatggc cgaagacgag cggaaacgaa ttaatagccg gcaaagagaa
     3541 ggcgtagaag ctgcgaaaca aaagggtgta aaattcggca gaaagaaaat tgctttacct
     3601 cctgaatttc ctgcgatcta ttctgactgg aaagcaggaa agataactgc agtctatgct
     3661 atggatcaat tgggcatgaa acgaaatacc ttttatcgac gggtgaaaga atatgaacat
     3721 accttgcacc acttttcttg atagatataa cagctattag acgggaaggg agagtttgaa
     3781 aatggactta acgaatcaaa gggtcgtt
//
LOCUS       DACDUU010000136         3661 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.136, whole genome shotgun sequence.
ACCESSION   DACDUU010000136 DACDUU010000000
VERSION     DACDUU010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.136"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..274
                     /locus_tag="IWL30_002810"
     CDS             <1..274
                     /locus_tag="IWL30_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HAP9707507.1"
                     /translation="LIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERINE
                     LIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            417..638
                     /locus_tag="IWL30_002811"
     CDS             417..638
                     /locus_tag="IWL30_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9707508.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            654..1496
                     /locus_tag="IWL30_002812"
     CDS             654..1496
                     /locus_tag="IWL30_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707509.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1559..3361
                     /locus_tag="IWL30_002813"
     CDS             1559..3361
                     /locus_tag="IWL30_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAP9707510.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
     gene            complement(3420..>3661)
                     /locus_tag="IWL30_002814"
     CDS             complement(3420..>3661)
                     /locus_tag="IWL30_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP9707511.1"
                     /translation="PPRKNMDKSEKLDHSLLGKQRKTVGTVFSSLEKLGCQNFNSRSV
                     KGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1305 a          519 c          745 g         1092 t
ORIGIN      
        1 actcatcaag ctggctaata agggtcaaga ttatgaacat ctagcagatg agattgatga
       61 gctgagagac aagcgacagc tccttttagt agaagatgcc tccctcagtg gcgagaacga
      121 gcgaatcaat gagctgattg aatttatccg caagaacaaa ttccgtacct tagagtacga
      181 tgataagctt gtaaggaaga taatccagag cgttacagtc tatgaagaac acttcgtcat
      241 atccttcaaa tctggcatcg aaatggaaat atgaaaacca gaagtaaata gcccatgatt
      301 ctacagtaga gtcgtgggtt ttcttttgct tgctgattat aataatattt taataactat
      361 tgttattata aaccaaatga tttataatat tcttagcgta aacttggagg tgctgtatga
      421 ctacggcaga aatgattaaa gaactgtgtg agcaaatgaa tataagtgtt tccgaacttg
      481 ctagacgtat tggacagact ccacagaatt tcaataagaa attacaacga gaaacggtaa
      541 ccttagatga gttgaaagcg attgctgatg tgctaggtgt caagtttgtg caggctttta
      601 tattgcctga tggcaatgaa ataaagatag gtaattagga ggtgtaattt gtattgaaaa
      661 tcataacaat aaatgatgtt gagtatgctg tatttgctgc taatgaaggt acttccaaac
      721 cacaacctca tatcatagaa acgaaatctg gtactattcc agaaggaaaa caactgagtt
      781 tattaaaaga atatcttaag cagaatgata tttccccaat aaaaggagca acaacatatt
      841 ggtgcattga caaggttctt aagcttgatt caagtaaaga aaaaactata agtgaaacaa
      901 tccataaaca aaaatactta tctttaactg aagaaaatat tgagaaacag cataagtttg
      961 taggtgcaag ctctaattat gggaaagaag gtctaataat acatgatgtt ttgaatgcat
     1021 ttcctcttca taatgattta aatactattg cgatgaagat tgcggtaatt gatgtaacaa
     1081 attcaacaca tctttctcaa tataagagca ggttgtctct atatgatttg gctaaagtaa
     1141 ttctggagat tccaaatttt gatgatcggt tggcaaaagg agatcctcag ttgataaata
     1201 ttatcgcaag gaacattggt gcagttaata tgttctcatt tgcatccaag tactgtacat
     1261 atcataatgt ggaagtgtgt gggagagatg attactccat ctttgatggt atcgttaaaa
     1321 atactcttcc acactatatc caaggactaa ctacgaataa gattgatacg tggcgaagaa
     1381 gttttgatta cgaggcattt aacgaatgtg ttggaaaact acttgatgaa aataatatac
     1441 atattccttt tagaagaaga aagttagatc acttcttgtg gtatgcgaat aggtagaata
     1501 taagaaaaag gcatcgtaaa agtgattctg gataaaatta atgggggagg aaataatagt
     1561 gggattattt gattttctaa agaaaaaaca aggaaaggtt agtaaacacg ttgattatca
     1621 actggcttct agtctatttc gtactggtaa agtttttgct gaaaaattgg aagtggacat
     1681 atcaaatgta gatgagtttc gtaaaacttt tatcgctttt gatgtggaaa caactggcct
     1741 aaattcttct tcagatagaa ttgtagagat tggtgcggtg ctatttaaag aaggagttcc
     1801 aattcaatca ttcggaactc ttgtcaatcc aaaagttaga attgcagcct cagcttcggc
     1861 aataaataat ataactaatg aaatgctaaa ctctgctcca aatgagcagg aagtttaccc
     1921 ccagttggta gaatttttag gagatgcact agatggaaaa atcactatgt gtgcacacaa
     1981 tgcaagtttc gattttggat ttttaagtaa cacattttct agacttgggt atgatgctaa
     2041 aattaagtat gttgatactc tgagtctctc tagaaaacat ataaaaggca ttgtaaatca
     2101 taagcaagag actgtagcgg aatatttcgg attaataaat gacactgccc atagagcgga
     2161 atcagatgca gatatatgcg gaaagatttt ttggggtgtt ttagatatta ttgaggagtc
     2221 gttggaagaa caaaaaaagc aaatagaaaa gatgactcca gacataaaag aacttgaagc
     2281 ttgtgctttt attcaagata ttattgagaa aaaaggatgc gacaccagtt ggatcaggta
     2341 tcgaaaaaac agtaataatt atgtcgatgt aacttgtcta tatacattct taaagtttaa
     2401 gtttgctaag aagggaaatt acatcattat tagaaaagat gctacaaatg gaattgattt
     2461 acctatagaa ccttgtactg taagtgaagg cggtacaaca aatgtgcgag tctattttcg
     2521 tagccccttt gacttaacgc ctctttcaaa ttatattttc agtgcttata gcgattgtta
     2581 taattctatg aatgaatata tgagtgttag taattacact aaaaaagaag cagagcaaag
     2641 ccttagtatg ctgaaatcta tttcaaaatc agcgatgaaa gagttattat tcgatgcaaa
     2701 aaatcgtgga tatgatgaga ctgaagttaa cctaaatatt gaaaaagtga tatctagaga
     2761 agatgtaatt attaacgggt tgcataatcg agtttcatta aacaagatac gaaatatagg
     2821 gaattgggag aaaggatttg atgcaggtta tccatattgg gaacgtggag aaactgctag
     2881 gaaaaatggc aaagtagatg aagctattgc actttttgat aaagctcgat ataacggcta
     2941 tgatgctcct gcgctatatg attcttatgc taaagcttat cgaaaaatta aggattacga
     3001 taatgaaatt ctcattcttg atgaaggaat tatgcgtaaa acacggcacg atgttggtac
     3061 tttagaggct cgtagggata aggcaattaa gctattattt gctaaacaag aggcagagcg
     3121 gatagctaag gaaaaatctg attttctaaa agcgaacaaa aaagaggatg cgattagtga
     3181 gcctagcaag aagcgtggaa gacagattat tcaaatgact gatgatggaa caataataaa
     3241 agaattcgat actatagctt ctgctgtaaa agaagttggc gttaactcaa aaagtattag
     3301 ggatgctgca aatggagttc aaaaacatgc tggaggatac tactggaaat acaaagatta
     3361 gcagataatc taagataacc taggatcttc ttaatagata cccgtggtgc tagttaattt
     3421 taatatccta aagaatatct caaagttcct tcaaaacgtc gttgtgctcg acttaataga
     3481 acactgtaag ccagtaatat gctttcaaat ctactctcta gccctttaac agaacgtgag
     3541 ttgaaatttt gacaacctaa tttttctaag gaagaaaaaa cagtcccaac tgtttttctt
     3601 tgttttccaa gtaagctgtg gtctagtttt tctgatttat ccatgttttt acgtggcgga
     3661 a
//
LOCUS       DACDUU010000137         3390 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.138, whole genome shotgun sequence.
ACCESSION   DACDUU010000137 DACDUU010000000
VERSION     DACDUU010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.138"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            64..297
                     /locus_tag="IWL30_002815"
     CDS             64..297
                     /locus_tag="IWL30_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707512.1"
                     /translation="MDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVFVYLVCSYL
                     LKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(456..578)
                     /locus_tag="IWL30_002816"
     CDS             complement(456..578)
                     /locus_tag="IWL30_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAP9707513.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            768..992
                     /locus_tag="IWL30_002817"
     CDS             768..992
                     /locus_tag="IWL30_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707514.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRKQLVIESMQIRNPDHFTRSMSI"
     gene            1435..1641
                     /locus_tag="IWL30_002818"
     CDS             1435..1641
                     /locus_tag="IWL30_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP9707515.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            1641..1892
                     /locus_tag="IWL30_002819"
     CDS             1641..1892
                     /locus_tag="IWL30_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707516.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEVLLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            2073..2345
                     /locus_tag="IWL30_002820"
     CDS             2073..2345
                     /locus_tag="IWL30_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707517.1"
                     /translation="MNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQ
                     ALELTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            2790..2969
                     /locus_tag="IWL30_002821"
     CDS             2790..2969
                     /locus_tag="IWL30_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707518.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            3028..3384
                     /locus_tag="IWL30_002822"
     CDS             3028..3384
                     /locus_tag="IWL30_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707519.1"
                     /translation="MLKTKKNSYKLKLSILSIICLLGMIISGHLYRVAYTQFIEYKVH
                     SLGLAGDRYVEYVHYSLKCSSYILLWLIFSLFELLNLIAISSYHLDKLNTKSSRYNEV
                     FKENVKLESDLNKLKK"
BASE COUNT         1192 a          558 c          660 g          980 t
ORIGIN      
        1 tgatgatcta ccgttttagt ttgagaaaga aggagtaagc atggcaaaaa aattagtctg
       61 acgatggatc tattgatacc cgtaattgca tttatagacg ggatttcttt gggcgtcact
      121 gaaaatcctt tggtaaatag aattgcgaaa ggatcactat ttcttgtatt cgtgtatctt
      181 gtctgttctt atcttttaaa aagagcagga aaggataatt ccttttggcg attgactaaa
      241 gtcacagtag cagcgacagc tgttatactt acggctattc taccgcttta tatgtagagt
      301 taacgggaaa tcgttgaact tcttgcgaaa tattgctata atgaacatga aaaagaggag
      361 ttgcgctaac aacccctcat taacagcacc gattaaagaa cggtggcaga atattagtta
      421 tttggaaata accgctgacc tgctaaagta gacggttatt tctttttgtc atttttaagc
      481 atatttacaa tcaaactaat caaagcaatg gtaaacatac caaaaccaag aatggtttga
      541 attgtctcat atgcggacaa aaggcttact cctttcatag attttagcac ttacattcat
      601 aagcaccacc acctttccag aagtcgccac cgtcttcaaa ctttactgtc ttgactatta
      661 tagcatatct aaaatcgaat aaaaattctc aattttagct gtttttgcta taattttagc
      721 gaggtgatcg tgatggaaca atatattggt ttactcgata aattagggtg atcaagacac
      781 agccattgct tgtgcgattt acattacgca cgagtcatga accaatcaat tgtgtagtgg
      841 ccaatataga aatagcggat aaattgctga ttttggaaga aggcaagtat actatttcag
      901 tgacaggtca cttcaataaa cgaaagcaac tagtcattga atcaatgcaa attagaaacc
      961 ctgatcattt tacgcgatcg atgagcatat aaaagaagag ctgcagtcct aaatgaggat
     1021 tgtagctctt tttgttttac atagaaacag taaaaatgaa aaggcctgtt taggaaggca
     1081 caagcgcctt ctcaattgaa agacctttct ctcactccaa ccgccaccgc cgttcgagaa
     1141 gaaaggaagt gaacgctacg cgttcactaa gataaagggt cttgcatagc aagacaaaat
     1201 agaaaaaatt tcctctcacc aaaaggttcg ctcctgttcg ttatctcttt attgatttgt
     1261 cagaccggca acaagttgcc ttttatcacc aaaaagcgac aaggacaagg gtaaatggca
     1321 cgctgcgcgc ccttgtcttg tcgcttttcg ttgattcatt ctggccaaca aatcaataaa
     1381 gaaagaacta acgaaaggaa gtaaaacctt tcctaagagg aggcagaatc aatcatgcga
     1441 aagaaatttg attattgggg tgttcccttc agcgaattat tcccaaacta tcatgcacca
     1501 catactgtcg aatgcgattg tggagaaaga gcgaaatgta tcaaaagcta ttgtttgtat
     1561 caatgtccaa catgcgggaa aaaatatacc ttgtcttatg gcgattatat tttaattgat
     1621 gagaaaggaa agaaacgctg atgaaaacac aacacaaatt attgactgta aaagaagtcg
     1681 ttcaaatttt ggtggacaaa gaagtcattt ctgaaaaagt agcagaagtt ttacttattc
     1741 cgcggtactg gacgatagaa acccctttag tagaggatgc accgactatg ctatttgaag
     1801 acctagtgaa gattgcagga tatgaactgc attatcaaga aaacttggaa acttatgaaa
     1861 acgaatattg gttaaccaaa ttagagaact aaaaaggaga aaaaaatatg gctcaaaatt
     1921 actatgacga atttgtcaaa ttacctttgg ataaaatggc acaaaaaatg gaggatatga
     1981 cgttcctata caacgaaaca cgtgttccaa aaaaacatta taaagaaaaa ttgtcggtag
     2041 ctgtggaaga aatgattgaa taaggtgtcg aaatgaatct tattgcgacg tactatcgaa
     2101 cactggaaga actgaaaaag caaaatgcaa aatggttttt ccaagcctta ctatgcttgg
     2161 aagtaggtgt caaaccaagc actattaaac cttctgaata ccaagcttta gaactaacgt
     2221 atgcgaaatt tatcgaaacg aaaaaagcca agactgtttc aagtgaatgg ttagattatt
     2281 ttgaaaatat caataagtat ggcgcatatt acaccatgaa aaaggaagac aacgaaaatg
     2341 aataaggtga tttatcaact aacaacggat tccatatcaa cgatttgaaa gaaaaaaagg
     2401 tcaataatca tttataccga aaagtgagaa tgtgtgaaac aaggacaaaa aaagcctagt
     2461 aactaaggaa atggttctta tttcgcacca atcgctccaa ccgcccccgc cgcttaggtg
     2521 gtgcgaaata aacgggcaaa gcccgataac ttaagagaaa gaaggtgatc catttgaaca
     2581 agattattcc cttattgtta gtactgtttg ttggcgaaat acttatgcga gatagtgcag
     2641 ctgtacagtt aaacctcgct gtatttttgg taatactggt gttttgtttg atcttttggc
     2701 ggtttgtatt agtcctcgtg agtctactat tcaagatgat tgcagccata tttttcatag
     2761 ggttatttcg atgaaaggag tgagtaccaa tgccaattgc aataggaaat aaacggttac
     2821 ccgtgacatt agatgaaaag agacaaaaag aattgcagca actaaagcag aagtatggca
     2881 aaagtgaatc taagattatg tgtattgcgt tagatctatt gatcgcgcaa gaaaaagcag
     2941 gatttgatgt accagcgcta aaaaagtgac gtcacttttt aatttgatct atataagtga
     3001 tatcactttt accaaggagg gaattaaatg ttaaaaacta aaaagaattc gtacaaatta
     3061 aagcttagta tactatctat tatttgtctt ttaggcatga taattagtgg tcatttgtat
     3121 agagtggcat atacgcagtt tattgagtac aaggttcact ctttaggatt agctggggat
     3181 cgatacgttg aatatgtcca ttattcatta aaatgttctt catatattct tctttggctt
     3241 attttttcat tgtttgaatt gctaaattta atagcaatat cttcatatca cttagataag
     3301 ctaaatacca aatctagccg ttataacgaa gtgtttaagg aaaatgtgaa gttagaaagt
     3361 gacttaaaca agctaaaaaa ataaaaagaa
//
LOCUS       DACDUU010000138         3249 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.139, whole genome shotgun sequence.
ACCESSION   DACDUU010000138 DACDUU010000000
VERSION     DACDUU010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.139"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            223..603
                     /locus_tag="IWL30_002823"
     CDS             223..603
                     /locus_tag="IWL30_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP9707520.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            585..1499
                     /locus_tag="IWL30_002824"
     CDS             585..1499
                     /locus_tag="IWL30_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP9707521.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            1459..1737
                     /locus_tag="IWL30_002825"
     CDS             1459..1737
                     /locus_tag="IWL30_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707522.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            1744..2433
                     /locus_tag="IWL30_002826"
     CDS             1744..2433
                     /locus_tag="IWL30_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707523.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(2554..2640)
                     /locus_tag="IWL30_002827"
     CDS             complement(2554..2640)
                     /locus_tag="IWL30_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP9707524.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
BASE COUNT         1155 a          606 c          652 g          836 t
ORIGIN      
        1 cttttttgta ttgtggagag tgggggggag ggggattcaa gggggagctt gcgccccttt
       61 gacaagcggt tatttgcacc aaatacgtag gtatttggct agcaaatata gagcttgcca
      121 agccctttag tgagctttag tgagctttac ttattgtacc tttttaggta caatggtatt
      181 gcgaacgata cgtccgtagg acgtactgaa agggtgggac ttatgagcga acaaaatcaa
      241 tatttggcta gcaatcattc taaaaaatat acctatcgat ccgagccaaa acaaatcagt
      301 tttcgagtga gcgaatccga atttgcaaag ctgaagcagt cagctgaagc tttgcaaatg
      361 agtgtgccgg ctttcgtgaa agccaaggca caaaacgcgc gtctggtgac accgaaagtg
      421 gcgccagaca ttgctcaggc aatcgcgcgc gatttagcca aagctggcgg gaatatcaat
      481 caaattgcca agtggtgcaa cacacaccaa cacgacgttg atcctggcga cgcccaacgc
      541 ttatccgaaa acctaaaaat catgcaaaag gagctacaaa agatatggca acagttaaag
      601 taagccgaac cacttcttgt agccgagcga tcaattacgc cgaaccacga gcaaccgtca
      661 aaacagggat aaactgtgac attgattatg ccaaaagcga aatgaaacaa attcgcatgc
      721 tgtatggaaa agacgatcgt gtacaagcac acttattgat ccagtctttt cgaccaggag
      781 aaattaccgc tgaaaaagcc aatcaactag gggcagagta tgcgaaaaaa atagcccctg
      841 accaccaaat cgcgatttac acacatacag acaaagacca tattcataac cacattgtca
      901 ttaactccgt caatctggaa actgggaaca aatttcaagc ccatggtcaa gcatttttaa
      961 acaaatgtta tgacctaaat gacgagattt gtcttgaaca tgacttaaca atcactgaac
     1021 gagggaagaa acccgaaaaa cgaaccatgt ctgaaatcaa attaaaagaa aaaaatgaac
     1081 ctgtttggaa agacgaaatt cggtttgcga ttgatcaaac aatgaaaaac aaaaaaactc
     1141 gaacgtataa ccaattttgt gattctctta aaacattcgg cattcattgc ttgaatcgtg
     1201 ggaaaaattt tacttacgaa ttgatccaca aaaagaaaaa agtacgatcc aacaaactag
     1261 gaaaggatta cgaaaaggag acgattttac atgagctgga cagacgagaa cgagcctaca
     1321 atcaacaacg aaccaacgag ctacgaagag ctcgagctag cattcgcgaa actgcaaaag
     1381 aagtacgaag agaacaacga actcttacga ctgaacaacc ggaaattaga ccgactcgaa
     1441 gaaaccatga ccaacacgtt gaaagaggag cagactacca acaagaatta tcgttctaaa
     1501 acgttacaag acatagaaaa tgaaataaag aaacaacgtg cttttcttgc accttatacc
     1561 aaagaaagaa tacaaactac aattgattca caagtcaaaa aattccacca tgcgctatta
     1621 aaccagcgaa ataaagaaca gaaaaaagta attatgcagc gatcaatgga tattcttgcc
     1681 cccttatgtt gcttaatcat gacactgtta gccttttggc tggtatgggg agaataaata
     1741 gaaatggcat tctatttacc ctatctactc atttttgcca gtattttagg aattatttgg
     1801 cttagctata aggcttatca aacaagatat tcattaaaag gctctaaaat tcgatttaag
     1861 aggtttttat tgccaggggt tatatttaca ttatcaatcg ttttaagtgg tttattcggc
     1921 gttttagagg gaaataaacg tatttcaaga agtattttat taggggatat cactcaaaag
     1981 tatgaagcag catgtgataa aaagaaaaag gagcaagtgt taacacaaaa aatggaagag
     2041 tttaccactt gttatgagga aatgaacgat atttttgtga atcaagaaaa acgtttgacg
     2101 gataaaaaca tggaaaaact cactcgtctt tatcaaaact tgccagaaaa acagcaaaaa
     2161 gaggttcaag acaactatga gcgaaccaaa aaagacgtgc aatattttaa agatacgcag
     2221 acagaagaag cttgttataa tttattcagt gatacaatcc ctttttcaac tagtgaacag
     2281 gaacgaaaag aacggcaaca atcagttacc tacgaacgtt ataaagcact gcttcagcaa
     2341 gcaacgaaca tacaaaatcc aacgaaaaaa gaaacggcat taaattatct aaaaagtgtc
     2401 aaggaatggt tagatcagca acagcaaaat tgaatttgat cggcaatcat gttaaactaa
     2461 acatgcaaat gatcaaacat tagatacacg aaagccccag agaggtcgga ttctctgggg
     2521 ctttttttca ggttgatgtc gcctttattg attttacttc ttgtttcggt tattcaacca
     2581 ataactgaat aaagagacac aaacttctat aagaaggggg caaatgaaca attcaaacat
     2641 tagatacacc tccttccagt cgcaagactg tcggataggc gacaagttat tatatcttaa
     2701 aaaattaaac ttgtgtggtg tttttaagta tcgcttattt tagggttgtt agggcttgcc
     2761 ctgaccgcct gtaaggcgct ttaaagctag ataaggattt ttatccaaga ctgtcttaaa
     2821 acggcttaga taagcattca gaaccgctca aaggcctctt taccaacatg tttttagcaa
     2881 aggagccttt aggcgactgc gcagtacaat gtgagagcgt agcgatcaca cctttttggc
     2941 gaccgtagga agcaaggttt tggattggca gttttgaaaa atgcgcgaac ctgtttaaag
     3001 aaaaaagaag tggtttttag gcgggaaaat tagcccccct ctttttatat ctctttttaa
     3061 acctcttttt aaacctcttt accgtaagct gtgtccttac tctaccaatg gatacagaat
     3121 gttatgaagg acattttgtc tgtttatgaa ggacattttg tctgtttatg aaggacattt
     3181 tgtctgtttg aacgtcaaat atgaatgtct tgacatttaa aaaatgtcat tcataattgg
     3241 attgaggtg
//
LOCUS       DACDUU010000139         2863 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.140, whole genome shotgun sequence.
ACCESSION   DACDUU010000139 DACDUU010000000
VERSION     DACDUU010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.140"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(185..472)
                     /locus_tag="IWL30_002828"
     CDS             complement(185..472)
                     /locus_tag="IWL30_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP9707525.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(492..716)
                     /locus_tag="IWL30_002829"
     CDS             complement(492..716)
                     /locus_tag="IWL30_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAP9707526.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(1356..1889)
                     /locus_tag="IWL30_002830"
     CDS             complement(1356..1889)
                     /locus_tag="IWL30_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP9707527.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(1910..2854)
                     /locus_tag="IWL30_002831"
     CDS             complement(1910..2854)
                     /locus_tag="IWL30_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP9707528.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
BASE COUNT          778 a          488 c          427 g         1170 t
ORIGIN      
        1 accttcggtg aaattgttgc aaatcaataa ttaggtagag ctacattttt ttcgttgttc
       61 aaagattctt atgccattta ctattaataa atctataaat atgataattt ttcggctcta
      121 taatttatgg gactgatgaa tcagaattga ttcttcagtc ccattttcat tttattccaa
      181 agaattaact ccaaatacca atagaagccc atcctctata tacttgagat tccttactaa
      241 caatttcttg taattctatt aaagatttag gtaatccaaa tttatgtttt aaaatatatc
      301 ttccaagaaa actacttaat ttcatactaa cttcaccaac actttttccc tcactgattt
      361 ctttagaata aagaaagagc ttttctttta agtcaggata gtcatctata gttgcttcat
      421 tgtatacttt actaattgca tttaaaagtt tttcttcttt agtaaaatcc attataataa
      481 cctccctacc actaccttct aggtgcccat ggaccatgat taacccaacc attaacaata
      541 atttttccaa tttctccttt agcttgattc caatctaccc aacatttttc tttgttacaa
      601 taaagaccat ttccatagta agtagctgca tcaactttta ttcctgtacc gatgccagct
      661 agacaagttc ctaatattcc tagaataaca caatgtttta atactttctt tttcatgatt
      721 gtttttcctt ttcgtttttt taaaattgct tgttatatta aataatctta gtaattttat
      781 tatataacat tttattatat aacaatgatt aacgtcataa aaagtagttt tgaattcata
      841 atcgtttaaa taaaattata agatcagtta tttaaacagc tagtagacat aatgtgtata
      901 ctaccaagta acgcctgtac caagggatcc aggcgctgat tttgtgaatt tttattggtc
      961 taggttaaaa gctcttttta aggcttggtt ttctgcggtt gtttctttct aattctttgt
     1021 gaatattgat cggtcatttt ttaaatactt gttatttttg tttgtgtttg ttatgctttt
     1081 tacatatctc atattcattt attattcttt tctaaggtta ataataggat aatctctttc
     1141 agaaagactg ctcgtacgag cagtcttttc ttatttatca agtttttcta caaatacttc
     1201 ctcattttga ctgttgtagt aattaggatt gagttttagt tccccttttt tatttccttt
     1261 gttggccact gataataaca ttgggctaaa gcttaatatt cctagtagga tagaaccgaa
     1321 tagcaagtgt ttttttgtca taagtatttc cttcttcatt tccatagctg ccaccatttt
     1381 ttccaattct ttgtttgttc gtccattgtt tctgttgctt cagaaagagt cgttgaacta
     1441 ttctctgaag actcttccgt ataagtcaac tgcagttgct cctgaagttt ttcgatttgt
     1501 tggttggctt gcagggtgag ttgttgttgc tggtcgagta gtttttgtat atgagaatta
     1561 ttttgctctt tgagggcaag ttgttctcgt aaaaattcat tttcttcacg caactgatct
     1621 agcaacttgt ttgcaaccac gtttttgttt ggtatataag catttttatc gttgttttgt
     1681 tcatgtttat gcaacttatc tagcaacctt tgattgtcta agttgcgagc aagttgcaat
     1741 actccagaac gcaatacaac cttataacca cgctcatttt tagctatcat ggaagaatct
     1801 aatttttgga tatgtttcga tattgcttgc ctactcatct taaaaagatc tgacaattcc
     1861 tttattgtaa aggtttcttg tggttgcata ctggttgcct cctaggttgc tactggaata
     1921 aattatctaa atcagataat aaactttttt tctctttttc cgaaagtgcg gactctgttt
     1981 gtttttcaag agcttttttc tctttttcta gttgtttgat ttgttggagg agttcaagtt
     2041 ctcgtttctt ctgtttgatt ttttcgttaa ttgttgtagc gggttcttcg tattcttttt
     2101 gtttatcagc taaagccaat gctttatttt ggtcttcttt ggttggtgca tgtttgttaa
     2161 agtcgtctgc ttttttgatc tcaggtttga acgtaaattg atagccaata acaggttttc
     2221 cccgtccttt tccgtatttc tttttgattg ttagtccttt gaagattgca gttagttctt
     2281 gacgtatagg ttttataact cgtttatcta tatcaggttg cttataactt ttaggaatat
     2341 ctaataattc aagaaagtct tctttggaga aatatgcgaa acctttggtt ctgtattgct
     2401 ttaacagacg aaacatagtc tttgcatagc cactttgtag ctcgttgaat tgttgtaaag
     2461 aatagcgtac ccactcgtct aaattattta acaaaggcag tgctttttcg taaattcgta
     2521 tatctacgta aggattttct acgtccccgt tgattttaaa ttctgtaaac attacaaaca
     2581 tatcgtaaga aagcccagac tcacttctac gaccaaagcg taaagataag attttttcat
     2641 aagttgcttt taaatcttct acaaatcgta catttgcagt tgctttgtag ttacttaaat
     2701 cttttagttg gtcaaacgta aaacgaactg tcttatcccc tttttcacgc attctagaca
     2761 cgatggaaaa aaacaaattc atttcaaccg gtgtaaattt tcttaaagga attgtattta
     2821 attcatggtg gtattttaca atctcattag ccatataatc acc
//
LOCUS       DACDUU010000140         2826 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.141, whole genome shotgun sequence.
ACCESSION   DACDUU010000140 DACDUU010000000
VERSION     DACDUU010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.141"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..158
                     /locus_tag="IWL30_002832"
     CDS             <1..158
                     /locus_tag="IWL30_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9707529.1"
                     /translation="GSLINNSSFMSNITTLGQSYLILISIIVLSVVFASSLMLIKKPK
                     EILSKIS"
     gene            168..824
                     /locus_tag="IWL30_002833"
     CDS             168..824
                     /locus_tag="IWL30_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9707530.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            824..2200
                     /locus_tag="IWL30_002834"
     CDS             824..2200
                     /locus_tag="IWL30_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9707531.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2486..>2826)
                     /locus_tag="IWL30_002835"
     CDS             complement(2486..>2826)
                     /locus_tag="IWL30_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HAP9707532.1"
                     /translation="TMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQARVT
                     SLEQLLSTVCDVGDISFSIRNQLRFKRSLPVSGNINVYFTKARFKRLIAITVSSIVMF
                     LWLFRMGVIP"
BASE COUNT         1104 a          350 c          498 g          874 t
ORIGIN      
        1 gtggaagttt aataaataat agtagtttta tgtcaaatat aacaacactt ggacaaagtt
       61 atttaatatt aataagtatt attgttttat cagttgtatt tgcttcttca ttaatgttaa
      121 tcaagaagcc taaagaaatc ttatcaaaaa taagttagga gaaaataatg gatatattag
      181 aaataaagaa tgtagcatat agttatgcaa attctaaaga aaaagttttg tcaggagtaa
      241 atcaaaaatt tgaacttggg aagttttatg cgatagtagg aaagtcagga acaggaaaat
      301 ctacacttct ttccttactt gctggacttg ataaacctca aacaggaaaa atattgttta
      361 agaatgaaga tatacaaaat aaaggatata gtaatcatag aaaaaataat atatctttag
      421 tatttcaaaa ctataattta atagattatt tatcaccaat tgaaaatatt agattagtaa
      481 ataaatcagc agatgaaagt atcttgtttg aactaggttt agataaaaaa caaataaaaa
      541 gaaatgttat gaaattatct ggtggacagc aacaaagagt agctattgct agagcattgg
      601 tatcagatgc tccaataata ctagctgatg agcctactgg taatctagac agtgttactg
      661 ctggagaaat aattaatata ttaaagacat tagctaaaga tagaaataaa tgtgtaatag
      721 ttgtaacaca tagtaaggaa gtagcagatt ctgcggatat cattttagaa ctaagtggta
      781 aaaagttgaa aaaagtaaat aaaatgaatt tggaggttga ataatgataa aaaatgcatt
      841 tgcttatgta actagaaaaa gtttaaaatc attaattatc atattagtta ttttatctat
      901 gtcaacttta agtctcatta gtttatctat taaagatgct acggacagag cttcaaaaga
      961 aacatttgct aatataacaa atagtttttc tatggagata aatagacaag taaatccagg
     1021 aacacctaga ggtgggggaa atgtaaaagg tgaagatatt aaaaagatat ctcaaacaga
     1081 tagtatagac tcctatgtaa aaagaataaa cagtgttgca gatttagttg attatgatat
     1141 tattgaaact caagagactc ttgcgaatca atcacctgaa agagcaaaga attttaaaag
     1201 aacagttatg ttaactgggg ttaacgactc aacaaaagaa acaaaatttg tatcagaagc
     1261 atataaatta gtagaaggaa agcatttaga aaatgaagat aaaaataaaa tcttaatgca
     1321 taaagattta gctgaaaaaa ataaccttaa agttggagat aagataaaaa taaaatctaa
     1381 tttatttgat gctgataatg agaaaggtgc agatgaaaca gtagaagtag aaattaaagg
     1441 tctattcgat gggcataata gcggtgtagt aagtgcagca caagaactat acgaaaatac
     1501 attaatcact gatattcata cagctgctaa agtttatggt aataccgaag atacagctgt
     1561 ataccaagat gcaacattct ttgtaaaagg tgataagaat cttgatagtg taataaaaga
     1621 tcttgggaaa ttagatataa attggagaga atataatttg attaaaagtt catcaaatta
     1681 ccctgcatta caacaatcta tatcaggtat ttactcaatt tcaaataaat tattttttgg
     1741 ctcattaata tttgcaggag ttgtagtttc attattatta ttcttatgga tgaatgctag
     1801 aaagaaagaa atagcagtat tactatcatt aggtatatca aaattagaaa tttttggtca
     1861 attcctaatt gagatggtat ttatatcaat ccccgcattc gttggttcct atttcttagc
     1921 tcaatataca gctgacaagc ttgggaataa tatattgaat aaggttactg gtgatatagc
     1981 taaacaaata gctaaacaat ctgcatctag ccaattaggt ggtggggcag aagctgaagg
     2041 atttaacaag acattatcaa gtttagatat caatgtatta cctaaattca tgatttatgt
     2101 agttatattt atgagtttag tacttcttgt atcattgatt atttcttcat tcaatatatt
     2161 aagaaggaat ccaaaagaat tattaattga caataattaa aattttcggt gctttttagc
     2221 accaaaaatg agagtgtaat ataaaccgtg taagtaacca tcaatcctag gcgggtggtt
     2281 gcttcacggt ttttattttt ctaaaataag atcaagaggt gattacacga tggctagaaa
     2341 gaaaagaaat cctgatgctg agaagttagc tgaatccatt ttgaatactt atcaacccga
     2401 atctgtagag gatatgcaag atgttttaaa agatgtgttt ggtcccttat ttgaaaaaat
     2461 gcttcaagga gaattgaata accatttagg gtatgacgcc cattctaaag agccaaagaa
     2521 acatgacaat cgacgaaacg gttatggcaa taagacgctt aaaacgagct ttggtaaagt
     2581 aaacattgat gttcccagag accgggaggc ttcttttgaa cctgagttga ttcctaatag
     2641 aaaacgagat gtctccgaca tcgcagactg tagacaaaag ctgttccaaa ctcgtaactc
     2701 gagcttggaa cagcttttta tctacttcgt ttcgaatttt tgaacaaaaa aataaaggct
     2761 ccttgccttt ccatagccag gtggctaatt tctttaaatt cattgcagca aacactagca
     2821 tcgtat
//
LOCUS       DACDUU010000141         2825 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.142, whole genome shotgun sequence.
ACCESSION   DACDUU010000141 DACDUU010000000
VERSION     DACDUU010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.142"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1386
                     /locus_tag="IWL30_002836"
     CDS             <1..1386
                     /locus_tag="IWL30_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9707533.1"
                     /translation="EVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTNTPGNYEGV
                     IEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPE
                     GTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQ
                     KVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEV
                     EVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVS
                     TAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEV
                     TVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKD
                     SGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGL
                     LGYKNRKKENE"
     gene            1520..>2825
                     /locus_tag="IWL30_002837"
     CDS             1520..>2825
                     /locus_tag="IWL30_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9707534.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNF"
BASE COUNT         1104 a          387 c          553 g          781 t
ORIGIN      
        1 gaagttgggg gcacagtaga tctaacagat aatgttacta acttgccagc gttaccagaa
       61 ggaacaacag taacagatgt tactcccggc ggtacaattg ataccaacac accaggtaat
      121 tacgaaggtg tcattgaagt aacgtatcca gacggtacga aagatacggt gaaagttcca
      181 gtagaagtga cagacaatcg ttctgacgcc gataaatatg aaccaacagt agaaggcgaa
      241 aaagtagaaa ttggaggcac agtagattta acagataatg ttactaactt gccagcgtta
      301 ccagaaggaa caacagtaac agatgttact cccggcggta caattgatac caacacacca
      361 ggcaattatg aaggtgtcat tgaagtaacg tatccagacg gtacgaaaga tacggtgaaa
      421 gttccagtag aagtgacaga caatcgttct aatgcagata aatacaatcc tgaaggacaa
      481 aaagtgacta cagacttaaa taaagagcca gatgcatctg agggaattaa gaacaaagaa
      541 gatttaccaa aaggaactac ttatgcttgg aaagagaaag tagatgttag cacagctgga
      601 aataaaaaag gtactattgt agtgacatat ccagatggat catctgatga agttgaagta
      661 gacgttacag tgacagatac tcgttccgat gcagataaat ataatcctga aggacaaaaa
      721 gtgactacag acttaaataa agagccagat gcatctgagg gaattaagaa caaagaagat
      781 ctaccaaaag gaactactta tacttggaaa gaaaaagtag atgttagtac agctggaaac
      841 aaaaaaggta ctgtagtagt aacatatcca gatggatcta aagaagaagt agaagttact
      901 atttctgtag tagataaaaa agcaccgaat aaacctcaag ttgatcctat tacagagggt
      961 gaccaaattg ttactggtaa aactgaacca aatgcagagg tgacagttac attaccagac
     1021 ggaagtcaac accatgggac agctgataaa aatggtaatt ttacagttaa agttcctaaa
     1081 ttagaggcag ggacaaaagt gatagtaact gcaactgatg aatctggtaa tactagtgaa
     1141 cctactaatg tagttgtttc ttccaacaaa aaagacagtg gaaaaaacgg tagtaaaggt
     1201 agtaagactg acaatcaagg tagcaacagc aatcaagaca aaaatagagg caaaagccag
     1261 tcctcaaaag tttttcctaa gacgggagag agtgacagta atatctttac tattagcggt
     1321 ggtttaatac tgttaggaac gttaggatta ttagggtata aaaatcgaaa aaaagagaat
     1381 gaatagaaaa tcaatttctt aagtcttctc tgagaagagg aagaatcttt ttcttcctct
     1441 ttttgctttt ttatgatggg aaataagtgt tagttaaaga tgaatatact ttttatagaa
     1501 aggaaaagaa tgtaattaag tggaaaaaat agtaattatt ggttcaaacc attcagggct
     1561 agccgcagct cgatttctaa acttaagcga ttcaaattat gaagttattc taatcgataa
     1621 aaaatcagaa attagttact tggaatgtgg gacatcgtta tttataaaag gagaagtaag
     1681 taatttagaa gatctttttt atcaaaatag aatgacacta aattctgaga taaaaaagtt
     1741 atttctagag acagaagttg acaaaattga tattcaaaat aaaaagatta gttgtgtaaa
     1801 aaaagatggt acaagattct ctcaagaata tgacaaagtt atattagcta caggttcaaa
     1861 gcaaattatg ctagatatac cggggaataa tttagctaat attttttcga taaagaattt
     1921 tccaaatgca aaagagattt ttaaagcttt ggatgatagt actattaaaa aagtaggaat
     1981 tattggagca ggatatattg gagttgaaat tgctgaagcc ataagaaaaa gagggaaaga
     2041 agtatatctt ttcgatgttg cagatagagt gttgtcgact tattacgacc gtagtttttc
     2101 agataaagtt gaagaaatac tatcaaaaaa tggaatacat ctttgtctat cagaaagtcc
     2161 taaaaaatat ttaggaagaa aaaagattga aaaaatagtc actacaagtg gtacccaata
     2221 ctctatggat atggtcgtac aagctatagg atttctatct aatagtccaa ttggggaaaa
     2281 ggaactcttg agagatgtta gtggagcata tctaactaat gaataccagc aaacaaatat
     2341 aaaagatata tatgctattg gggattgtgc tacaactttt tttacgtcca ttaatagaag
     2401 tagtgttgat ttttctattt ctaatgctct aagaacagct tatattgcaa ctcaacatat
     2461 taacaatcaa gattttacac cttctggttc ccaatttaca aatggctttt ctatatttaa
     2521 ttacaatttt tacgcagcag gtttgaattt aaaaacagca cgattattaa gtgagtctat
     2581 agattatata gaaatagaag atttcattag acctgctttt ttgagaaaaa atagtcgtgt
     2641 aaaattaaga attagctttg aaaaaaatac aaagagaata gtaggtgtac aactttgtag
     2701 tcaagaagat ttatctggac tattatcaat gttttctctt gctatagaag aacaaattac
     2761 attagacaaa ttgaaaatgt tagattattt atttcatcct agttttagcc aaccctataa
     2821 tttta
//
LOCUS       DACDUU010000142         2712 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.144, whole genome shotgun sequence.
ACCESSION   DACDUU010000142 DACDUU010000000
VERSION     DACDUU010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.144"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(1..970)
                     /locus_tag="IWL30_002838"
                     /pseudo
     CDS             complement(1..970)
                     /locus_tag="IWL30_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
     gene            1179..1553
                     /locus_tag="IWL30_002839"
     CDS             1179..1553
                     /locus_tag="IWL30_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9707535.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(1667..2296)
                     /locus_tag="IWL30_002840"
     CDS             complement(1667..2296)
                     /locus_tag="IWL30_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9707536.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(2430..2502)
                     /locus_tag="IWL30_002841"
     tRNA            complement(2430..2502)
                     /locus_tag="IWL30_002841"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2467..2469),aa:Thr,seq:ggt)
BASE COUNT          857 a          439 c          456 g          960 t
ORIGIN      
        1 aattctactt tcaacgactt ataagttgat tcagctacgg cattatcaaa aggacagcct
       61 ttatgactca atgatcgatt gatgtcaaaa gttgttaata attcatcaat agcttggtta
      121 tcaaactctt ttccacgatc agtatgaaaa atctcaacct ctgtcagagg ttgtttgata
      181 cggctaaatg ctttttttac tagaacggca tctttatgtt ctccacaaga atagccgaga
      241 atttctcgat tgaacagatc caaaatgaaa cagacataat tccatttttt cccgactcgt
      301 acataagtca agtctgttac gatcgcttct aatgggttgt ctcttaagaa tttacgattc
      361 aatacgtttg tcgttttggc ttcattgcaa gtagaatgat gtactttaaa ataagcaaca
      421 gtatagctcg attttaatcc tctatttttc atgattctac taatttttcg tcggctgatc
      481 tgaatgcctc gttttgataa ggcttttttt atttttcttg agccgtaggc ctttcggctg
      541 cggataaatt cttcagcgac tacttcttca agttctgatt cgtctttctt tggttttgat
      601 tgataataat aggactgacg tgatagacct aatattctgc acatcgctga tatagggtaa
      661 agatgcttat tcgcatcgat tacttgtctc ttcgtccgaa tatcagcgct gcttgcttta
      721 aaatatcatt ttccatttct aattgctggt ttcttttacg tagttctaac aattcttttt
      781 gttcaggcgt aagattatct ttttctttga atgaaccact cgttttagat tgctttaccc
      841 atttgtcaaa tgctgaagcc gttagttcat attctcgaat gatttctaca cgtggctttc
      901 cagctaagta aagattgacg atttgttgct tgaattcttg tgaaaaagtt cttcgtgttc
      961 tcttagacat aaaaattcct cctggtatgt tttcttctag tctacacacc ttaatttttc
     1021 tgtctagtta attgtagcct atccaagtct acactctgag atgtaagaaa tatcataaga
     1081 tatttcttac atctttttgt tttgagcagg actttagata attttattaa tatatgctag
     1141 catgcttttg taagaaaaag aaagagggcg tattagttat gtatcttaaa attacggaac
     1201 aagcacaaga aagattacaa aaatatattg atgaaggcgc aacagttata ttggatttag
     1261 atgatggcgt tggggaatat tctaaaatgg gtgtttgctc tttagatact agttttcgtt
     1321 tacttcttct tgataaagaa cagagaaaaa atgattataa actccaatta gatagtgata
     1381 ttggggatgt ttatataaaa gattattcaa aaatgtacat ggatgaacat atgacccttt
     1441 ctttagatcc acgtctaggt gtatttactc ttgaaagccc aagtggttct ttagacagtc
     1501 atgttcaatt agtagatcta cgaccagaaa aattctctgg aataaaacaa taactttctc
     1561 tctattgtcc ataaaactag tctcaatcaa gctgtattga aaaaacaaga caacattctt
     1621 accatggtaa ataaaaagac gacacatttt gtcgtctttt tttaaattaa ctattttcga
     1681 taaaagatcg catagcttgt gccattattt ctggcttttc tctattcaat tcatgttttc
     1741 cgtttttaat aaagagtagc cggccattcg ttgaaagttt atataaacgt tttgctgctg
     1801 gtttattcgc atgatctttg gtaccgcata aaatcaattt aggaagtcgt gatgtacgaa
     1861 gctgtttttt gcagtctaat gtttcaagtg aacgaagtag ggtaagggtt tgttttttag
     1921 atagacctaa tttttcaaat atcttagaag gtacgcagta aaaataaata ttctggaaag
     1981 taagcaataa gcgattagga gactcaaatt gtggagcgac taaaacaacc ttttttaaat
     2041 atggagaagg agttgcaaga tagtttaaag caataacagc gcctaaagat aatccacata
     2101 aaataaaagg ttcctttatc tgattcaatt gtacttcgat gtatttggtt aagtcgtcta
     2161 aagtagcagt gtctgtgctg tttaaatgat tgaacaatga aagacttttg caagaataac
     2221 tattgggtag ataagagatt acatctttcc atgattgttc gtcttggcct agaccatgca
     2281 aaaaaacaat tttcatattg ctctcctttt tatagttatc atatagactt tctttgtact
     2341 attaaaatat aaacattcat ttcttacgtc ataaaataaa aaaacagcgc agacacattg
     2401 ttaaaccaac atgttgcact gtttaaggaa gccacttgcc gggcttgaac cggcgacctc
     2461 ttccttacca tggaagcgct ctaccagctg agctaaagcg gcattatcat taatacaaat
     2521 aataggataa cgaatattaa tgtatttgtc aacagaattt tttcgaaaaa atgtgtttct
     2581 ctaataggaa aacagatttt agaaaaaata aaaataacaa aaaatgcgaa cggtttcttt
     2641 gataatataa taaaaaaatt caaaaaaagt attgcgttat ttagaagaaa ttgatattat
     2701 attcatgtac tt
//
LOCUS       DACDUU010000143         2653 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.145, whole genome shotgun sequence.
ACCESSION   DACDUU010000143 DACDUU010000000
VERSION     DACDUU010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.145"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            439..759
                     /locus_tag="IWL30_002842"
     CDS             439..759
                     /locus_tag="IWL30_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP9707537.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGLLLISIIGIKI
                     TSGH"
     gene            920..1825
                     /locus_tag="IWL30_002843"
     CDS             920..1825
                     /locus_tag="IWL30_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9707538.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSEGTIRIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVKQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDQAARTILTDAGYPLEDPDKIKLLKKNEELLGTVIQLLSAENVHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            1822..2595
                     /locus_tag="IWL30_002844"
     CDS             1822..2595
                     /locus_tag="IWL30_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9707539.1"
                     /translation="MTLLIKQELFKLIKKKSTAVLSVLLVALLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMESQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSIYLYLLAIIVTVLMKLILFPSISFTEKISTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIALFLALGYLVFKRKNV"
BASE COUNT          831 a          462 c          494 g          866 t
ORIGIN      
        1 attataaagc aaaacacata aaaaatatag ttgttttctc acgaaataga cactattttt
       61 aaatattttt cgttattttt ttattatata attcacctta cttattgtaa gataaagctt
      121 tttttaattt attgcctgct aatatataaa aataaggtct agttactgtt tttttaaatg
      181 attaataaat aacaactcct ttctatgaaa ttgcaaagca ttcatcttct tttctgctaa
      241 cgatatatcc gtcaatgctt gttacacttt actaaaaagt atgagattgc cgatatctgg
      301 ataggtgtgc tatactacag aaaaagatat acgatctgcc accgggtagt aaaaaagagg
      361 atgatcattc cattaggtct ttgaagggat gattctcctc tttttgtgta ttttttatat
      421 tgaaggagga acgtatgtat ggcatggatg gaattaatca tagcaggaat tttggaagta
      481 ttctggtcaa cgatgatgaa atggagtgat ggtttttcta aaatcaatta ttctttttac
      541 acagtgattg gaatgattgc tagtttttac tttttgtcaa aggcaatcaa aagtttgcct
      601 atgagtctct cttatcctat ttggacaggg attggcgcag ttggttcggt cattattggt
      661 gtggtcttgt ttcatgataa gctgaatctt tccacttggt tctttgtcgg gttgctgctc
      721 atcagcatca ttggcatcaa aatcactagc ggtcactaga tacttagaca agcagacaag
      781 gactgatcag aaaaagcggg gctttttatt tgatattcct ctttctatga aaaaacaaga
      841 aaataagact aatgacctag cgacttctct actcatcagg tgtatgcttg tactatctat
      901 agaaaaggag tgaatatttt tgaaaagtct ttcggtccaa gaattatcca agaactttgg
      961 tcacaaacaa gccctcagcg atgtcagttt tagcttatct cctggcaaaa tcgttggatt
     1021 agtaggtcct aatggcgctg gaaaaacaac gatcatgaaa gctattttag gactgatcca
     1081 ttactcagaa ggaacgatcc gtattggtga aaatgagatt tctccctcta gtcataaagg
     1141 attagaacaa gtaggagcat tgatcgaata tccgggtatc tatccattct taacaggtta
     1201 tgatcatctg aagttattct ctgaaacaaa cgatgtatct gctatcgaca ctatcgtgaa
     1261 acagctcaag atggaaaaat acattcacaa aaaagcgaaa tcctactcgc tgggaatgaa
     1321 acaaaaattg ggaattgctc ttgccttact gaaccatccg tctatcgtta ttttagatga
     1381 gccgatgaac ggtttggatc cacaagcaac aagagatgtg cgaaaaatca ttaccgactt
     1441 ggctactcag ggcacctctt tcttgatctc cagtcactta ctcagcgagc ttgaaaaaat
     1501 ggtcgatgag ctgatcctga tcaatcaagg aaaaatcatc aaacaatgta caatggctga
     1561 attgaaccaa acagcgcaag atttcatcgt tatctctacg acaaatgatc aagctgctcg
     1621 aacgatcttg acagacgctg gatatccctt ggaagatcct gataaaatca agcttctaaa
     1681 aaaaaacgaa gagttattag gaactgttat ccagttattg tctgcagaaa atgtccatgt
     1741 gacagatgtc cagcatctga cgaatgattt ggaatcttcc ctattggaaa ttctggattc
     1801 agtaaagggg gaagaagaaa aatgacgttg cttataaaac aagaattatt taaattgatc
     1861 aagaaaaagt caactgctgt tttatctgtt ctattagttg ctttgctcat aggaacagca
     1921 cttctagcca aaaaatacac gacgatcatt gatccagttg aaatgactgc tcagcttttc
     1981 agtgccacat cttggattgt ctttatcatg attgctgctg caagtacgat catttcaatg
     2041 gaatcacaat atggtacatt gaaaaatctc ctttatcgaa agtattctcg aggagaaata
     2101 ttagtaagta aatggatcac attagttatt tattctattt atttatacct tttagctatt
     2161 attgtcaccg tgctgatgaa actgatcctt ttcccttcga tttcctttac ggagaagatt
     2221 tctactggtc agacactgat ccaaagctta ttcacttata ctttgggtag ttatatcggt
     2281 ttatggctaa ttttaagttt agtgttgatg attgcttgtt ttatcaatag ttccggtgcc
     2341 tccatctcag cagggatcgt cttttatttt gcttcatcaa tcatatctgg tatcctcatc
     2401 gcactcattc aaaaatggga gtggatcaaa tggaatccaa tcagtatgct gaatctgcaa
     2461 aaccaaattg gcaacgaaga gattatgaag caattgactc atctttctac taatcaaatg
     2521 ctgtttggca atttagctta cattgctttg ttcttagcat tgggatacct cgtattcaag
     2581 cgaaaaaatg tatgatggtt cattaacaaa gaagttgtga cagaagtggc cagtttcaag
     2641 aaatttcggc tta
//
LOCUS       DACDUU010000144         2584 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.146, whole genome shotgun sequence.
ACCESSION   DACDUU010000144 DACDUU010000000
VERSION     DACDUU010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.146"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            328..2493
                     /gene="acm"
                     /locus_tag="IWL30_002845"
     CDS             328..2493
                     /gene="acm"
                     /locus_tag="IWL30_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAP9707540.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
BASE COUNT         1031 a          368 c          485 g          700 t
ORIGIN      
        1 ctaataatgt taattaaaag tgtattttat gaaactatcg ttatttatta atgttgcatt
       61 tttgtaagtt aaaaattgga aatatagatt gaaaaaatga aagcaataag taaaataaaa
      121 tgaatatatg cgaaaaaata atttacatta ggaaaaacct tgtaaaaaca gttttttggc
      181 atatttattt tttataaata tttgtaaaat aactatcaat tttttagtta aaagattgga
      241 aatgagtggg ggaggaattg aagaaaggat ttttgagaga tgatatagta ggttttattt
      301 ttagttaaaa taggggagat agaaaaaatg aaaaagtgta gaaatattat tttatcaatg
      361 ttatttatta ttactaatat aacatcactg attcctgttc atgtctatgc ggatgcaggc
      421 agagatatca gcagtaatgt cacttcgttg accgtggacc caacgaatat tacagatgga
      481 ggaaacataa aagtaaaatt ctcgtttgac gaaaaaaaac aaaatatcca accaggtgat
      541 tatctttgga tcaattggcc aagtgaagga aatattcgcg gtgaaggatt ccaaaaagaa
      601 atcccattaa tgattgaaaa taaaaatgtt ggaactttaa cggtcagaaa agattctgcg
      661 caggttgtgt ttaatgaaaa tattaaaaac ctagactcag tagaaggttg ggggcaattt
      721 gagatacagg ccagaaacgt aaccgatacc ggtgaagaaa atacgggacc attcaccgtg
      781 acgagcggtg ataaaacagc tactgtaaat gtgacaaagc cagcttctgg ttcttcttct
      841 agcgtttttt attataaaac aggagacatg ctacctgaag atactaagca cattcgatgg
      901 tttttgaata ttaacaataa cggcacttat gttgaacaac cagttaaaat cagtgatgaa
      961 atccaaagtg gacaaagatt agatccaagt acgtttgaaa ttaatcaaat acacttaggt
     1021 gaacagaagg tttatagagg agaagaggga atccaacaat ttttacaaga tttccccagc
     1081 gctacattta atttttcagt aactgataat tacattgaaa taactatacc aaaaaacttt
     1141 gtcaatctta ggaaaataat ggtgagttat aagacgatca tagaaaaccc agaacaaata
     1201 aattttgaga atcattcgga agcttggttt aaagaattta ataaacctgc tgttgatgga
     1261 gaaagtttca atcatacagt taaaaatatt tcagcttctg gtggagtcaa cggtacagtt
     1321 agaggcgaat tgaagatttt caaatatatc aatgacacag agattggtat cccaaatgtg
     1381 actttcgaat taagacgagc agatgaacag cctattcaag gacagtctag tatcctgttg
     1441 acttcgaatg aacagggaga agctagtatc aaaggcctgc aagtaggaga ctatgttgta
     1501 aaagaaaaag aagcacccaa ttggattgat tttgatccac taagttcaaa tgaactcaaa
     1561 ttctccataa acgaaaatga tacagaggga gtcagtttac ctatttataa caagaaaaag
     1621 gttacgaaca tcacagcaac taaaatttgg aacggtggaa ccactccaag accgagtatt
     1681 tattttaaat tattcagagc aagtacaaac aattgggaac cagttcctga tgcagaaaca
     1741 aaacgattag acaatggaat aacttcagtg acttgggaag atatccaaca gtatgatgat
     1801 agtggaaacg aatatacgtt taaagtgcaa gaagtagatc aaaacggaaa tgattatgtc
     1861 ccttcaggtt atcaaaaaat tgaaaacggg ctagtcgtta caaatgagaa taaagaagtc
     1921 atttcagtct caggacaaaa aacatgggaa gacaatgaga accaagacgg caaacgaccg
     1981 acgacgatca cagtaaatct attagccgac ggtcaaccga tccaacacaa agaagtaagt
     2041 gaaaaagatg attggcgcta tcgtttcaca aatctgccaa aatacagaga cgggcaagaa
     2101 atcatctata ctgtaacgga agacaacgtg cccgaataca gtacgacgat tgacgggtat
     2161 aacatcaaaa acagttatac accggaaaaa acaaatatat ctataccagg tagccatata
     2221 acaccgtgga aaaattttcc agagaagaca gagagagaga aaattagaaa tctattctct
     2281 caaaaattgc aaatatttgc tcatacagga gaatccagcg aacaaagaag caattatatt
     2341 agtaagagag ctgtacctaa acagataaca aataaatcaa aaagcattct tcctaaaaca
     2401 agtagtaaaa aatctagttt tgctgtaatc attggtttat tgatagtaac tatctgtgct
     2461 gggatattct tacaaaaata taaaaaagtt tagttagaat ataatatttt ttgttaaata
     2521 tgactgaaat caaaagaata aaattccgat ttataatcga aaaaagaagc atttttatat
     2581 atgc
//
LOCUS       DACDUU010000145         2497 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.147, whole genome shotgun sequence.
ACCESSION   DACDUU010000145 DACDUU010000000
VERSION     DACDUU010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.147"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(526..1866)
                     /gene="glnA"
                     /locus_tag="IWL30_002846"
     CDS             complement(526..1866)
                     /gene="glnA"
                     /locus_tag="IWL30_002846"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAP9707541.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(1894..2286)
                     /locus_tag="IWL30_002847"
     CDS             complement(1894..2286)
                     /locus_tag="IWL30_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9707542.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          720 a          550 c          418 g          809 t
ORIGIN      
        1 tcgtgcctcc ttatttctaa ataaacggtg ttctcctgcc aactcctcgg tcacaagtca
       61 caatatccat caaacatgag aagctgtttt tgaaattatt tcttgtgatt cggagttaaa
      121 tggtaggctc acaacctctg gataaacggt attcaaaagc cagtttctcg gtattaagcc
      181 aaatttatgc aaaaatgtag aaagccattt tcacaaaatt tttcttaata ctcgaaacta
      241 aacgtctttt tcaaaacctc ttggtaaacg gtgttcaaaa gcttgcatct gcggtaataa
      301 gttcagccaa ttcaaaaaca agaaaagctg tttttgcttg gctattctta ttacttgttg
      361 cagggcagct ttttcacaac ctctccacta aacggtgttc aatcagctga ccctcggtat
      421 tagctcaaaa aacggcaaaa tatgaggaac tatttttccg ttagtttctc taatactcag
      481 gtcaaaacgc tgatttcaca gcctctaatt cacggtattc ctgttttaat aaagttctag
      541 atattgttct ctttcccact cggaaacagt ttgtctgaat gccgcccatt cgagacgttt
      601 tgcttcaaca aaatttgaga agatatgttg tcctaatgcg tcaatcatta cattatcctt
      661 acgcaattct ttgatagcat tatggatagt tgaaggtaag tcctggatat gtgcagcttc
      721 tctttcttcc tcatccatta cataaatgtt acggtcaaca gctgctggcg gtgtgatctg
      781 attacggata ccgtctagtc ctgcttgcaa caatactgcc attgccaagt atgggtttgc
      841 agatggatca actgaccgca attccaaacg tgtggataaa ccgcgagatt ctggtacacg
      901 tactaatggc gaacgattac ggccactcca tgcgacataa acaggcgctt cataacctgg
      961 aacgagacgt ttataagaat tgactgttgg attgcaaacg gctgtgtagg cacgagcatg
     1021 tttcagcaaa ccacctaaga aatgataagc tgtttcactt agttccaatt caccgtcttt
     1081 atcatagaaa acatttcctt catcattgaa caaagacata ttgcaatgca taccagaacc
     1141 attgatccca aataacggtt tcggcatgaa agtcgcgtga agtccatgct tgcgtgcaat
     1201 cgttttgaca acgagcttga aggtttggat attatcacaa gcatctacca catctgcata
     1261 tttaaagtca atttcatgct gtcctggcgc tacttcatga tgtgaagctt ccacttcaaa
     1321 gcctagactt tctaattcca acacgatgtc acggcggcaa ttttcaccta aatctgttgg
     1381 tgcaaaatca aaataaccac ctttatcatt caaggtcgtt gtgatcccac cgtcttcgtc
     1441 taatttaaat aagaagaatt ctggttcagg tcctaaattg aaagaggtaa aaccaaggtc
     1501 gcgcatatct ttcaatgctc gtttcaaatt cccgcgagga tcgccggcaa atggtgtccc
     1561 atccggatta tagatatcac aaattaaacg agcaactttt ccatgagcac tttcccacgg
     1621 gaagatcatc caagtggaga gatctggata caaatacata tcgctttctt caattcgaac
     1681 aaatccttca atcgaagaac cgtcaaacat cattttattt tctaatactt tgtcgatctg
     1741 gctcactgga acttctacat ttttgatcgt acctaagatg tctgtgaaca tcaatcgtaa
     1801 aaatcgtacg ttttcttcct caatgattcg tttgatttct tcacctgtta tctgtttctt
     1861 taccatggta tccctcttcc ttttttaatt tattcagtca aagaattgtc tttgcaatcc
     1921 ttgcgactga taatgacctg ttttgcttaa cccaccttgc gccaaaatct catcacgaaa
     1981 aatttgtcga acgtcatgat ctgtcaacgg cttcttgttt tttgcttctt gttttttcgc
     2041 ctgtgccatt tcgtatacac gtttgatccc agccatattc aatccctctg aaagatagtc
     2101 tttaatctca agcaacatat caatatcatt cagcgaatac atacgtcggt ttccttcgct
     2161 tcgttccgga tgaactaaat cttgttcttc ataatagcga atttgccgag cagacaaatc
     2221 agtcagcttc ataactgtac caattggaaa tacggacata gatcggcgca attccttttc
     2281 tcccatcaga cgtccccttt cgttttataa gttggctaaa gtgtaccaat taaaaaaaat
     2341 attgtcaact gcatatgtta ggttttctaa catgagatct ctatttttta taaaataaca
     2401 aaaaaataag aacacaaaaa aggtatctag ttgattttcg taccagatac cttttatttt
     2461 ttattatact gtactatcct ttattagtgt ttttact
//
LOCUS       DACDUU010000146         2483 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.148, whole genome shotgun sequence.
ACCESSION   DACDUU010000146 DACDUU010000000
VERSION     DACDUU010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.148"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(1014..1304)
                     /locus_tag="IWL30_002848"
     CDS             complement(1014..1304)
                     /locus_tag="IWL30_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707543.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1512..2203)
                     /locus_tag="IWL30_002849"
                     /pseudo
     CDS             complement(1512..2203)
                     /locus_tag="IWL30_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          734 a          458 c          348 g          943 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattac tgttgcttct ttactaattt aggtttactt
     1501 gttaagaaag attatttacg caaatgttct tcaacaattt gctgagattt atcagctgta
     1561 tcttccactt catcttttgc tttttctaac ttttgaatat attgtttcaa gcttgagttt
     1621 tcagcagttg cttcgtctaa tttcttttgt gtatctgtta actgatttct acatttgttt
     1681 tttcttgtgt cagtgttgcg atttgttgat tcaaaccatt tacagtattt tgtaattctg
     1741 aaagttttgc ttgtgcttct tgtagtcttg ctgtccaatc tgcattcaga tgattgattt
     1801 catcattttt ttggttgatc tcatctagtt tgacattgat ttcattttgt ttgctttgta
     1861 gttgtgcatc cttgctgact aattgacttt ctaagttttg tttttctgct gccgattgat
     1921 ttttttggtt ttctaattca tttacttttg tttggtattc ttggacatct gcctttgaat
     1981 cttgcaatgt ctgttctttt tgtgctagtc gagcggtaag atctgtaatc gcttgctctt
     2041 tatctgtcaa tgtatgatca agctgtgtca gattctcatt gattgtatca atatctgtct
     2101 ctccacccca caaaataatt tcatttgcgt accgcgtgcc tgtgtagcca attagacata
     2161 gaaatgcaac gacaatccca ccagtaataa atttcttttt catttgcata gctccttaat
     2221 ccaatacttt tacatgctaa aagtaagtga tctcaaacac aatgtcaagc gtttttaaat
     2281 tacatatgta attttaatat aaatactatc aaatactcat ttaatcctcc ttttttatct
     2341 atttaaaaaa tcttttttat agagaaaaat gcaggttaat actttcttaa ataaatattt
     2401 atggtgaaca aaaacaaaaa agcaagtttt attaaacgta acccttttca tttcatagat
     2461 tactgtattt attacaaaaa acg
//
LOCUS       DACDUU010000147         2419 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.150, whole genome shotgun sequence.
ACCESSION   DACDUU010000147 DACDUU010000000
VERSION     DACDUU010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.150"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            6..1150
                     /locus_tag="IWL30_002850"
                     /pseudo
     CDS             6..1150
                     /locus_tag="IWL30_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
     gene            1321..1779
                     /locus_tag="IWL30_002851"
     CDS             1321..1779
                     /locus_tag="IWL30_002851"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAP9707544.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..2408
                     /locus_tag="IWL30_002852"
     CDS             1938..2408
                     /locus_tag="IWL30_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAP9707545.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          810 a          454 c          456 g          699 t
ORIGIN      
        1 gaaacatgca cattcaaaca aaattgatcc acggaggcat cagtgaagat cccacaacag
       61 gagctgtcag tgtacctatt tatcaaacat ccacttatcg gcaagatggc gtcggccagc
      121 ctaaacaata tgaatattca agatctggta atcctactcg ttttgcattg gaagaactga
      181 ttgctgattt ggaaggcggt gttcgaggat tcgctttttc ttctggtctt tctgggattc
      241 atgccgtttt ttctcttttt caagctggcg atcatatctt attaggagat gatgtctatg
      301 gaggaacatt ccgtctattt gacaaagttc ttactaaaaa tggtctcgag tacacgatca
      361 tagatacgag taatcttgat aaaatagaac aatccatcaa acccaataca aaagcacttt
      421 atcttgaaac acctagcaat ccgcttctaa agattactga tttagaaaaa agtgccactc
      481 tggctcatca acatggattg atagttattg ctgacaatac atttgctact ccttattttc
      541 aaagaccttt agatctcggt tctgatatcg ttgtccatag cggtaccaaa tatctaggcg
      601 gacacagcga tgtagtagct ggtttagtga cttctaacca aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttctttaact taataaaaa
//
LOCUS       DACDUU010000148         2406 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.151, whole genome shotgun sequence.
ACCESSION   DACDUU010000148 DACDUU010000000
VERSION     DACDUU010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.151"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..103)
                     /locus_tag="IWL30_002853"
     CDS             complement(<1..103)
                     /locus_tag="IWL30_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9707546.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQEL"
     gene            complement(177..458)
                     /locus_tag="IWL30_002854"
                     /pseudo
     CDS             complement(177..458)
                     /locus_tag="IWL30_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            920..2302
                     /locus_tag="IWL30_002855"
                     /pseudo
     CDS             920..2302
                     /locus_tag="IWL30_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          875 a          412 c          400 g          719 t
ORIGIN      
        1 acaattcctg aacttcacga tagcttagat tgtaacgcag gtagtaacca acagcgacaa
       61 taatgacgtc ttttttgaat tgtttgcctt taaaatgatt cattactctg tcctctctgt
      121 cttttttctc aattttacac taaaatagat tttttggaaa actttgcaac agaaccgaaa
      181 tgagtatagt atgaattagt aatttggttt tcatctaagt ttagctttga tttactaaaa
      241 ttgatatctc gatacatgtt caatacatct aatacaaatc gagaatcttc tttcggaaat
      301 ggctcagaaa gatcactcca aatttcatca tcataatgat actcgtaacc ttctctcaat
      361 atttcgattt ttctttcata ataagatctg ttttggtcct caaatttagc caataactca
      421 aattgattta ctagtgacaa tctctgaaaa ctgtccatta aatgtttctt cctatcttgt
      481 tcattaacaa aaaattataa gcccttacaa cgaatattat atattaaatt taattgtagg
      541 tgaacacgaa ttttataaat atttatggtg ttaatgacaa atgatatccg actcctcttc
      601 atacgttctg gactatttgc taatagatta aaatttcagc atctatttct gaccataaat
      661 aaatgagctg ccgaaaagct gccaaatcag acttgaaaat aaaggatcaa aaacttttaa
      721 gtttctgacc ccgtaactca cacttcaaaa tcttacttaa aaaggagtct gaaagaaaaa
      781 aagagtattc aaaatttttt tctaactagc tacatcacat ctatgcattg acactcaaag
      841 aaccagaata agtcatcaga aagcaagggg gtgcggacat aatcctggac gaaaaaataa
      901 acttaggagg aatgtccgtg tgttttccca caaagagcgt gtaagggcaa ttcagttatt
      961 tttaaaatac gattgctctt atgctgcaac cattcgagaa ttagggtatc cgtcgattgg
     1021 tgcactacgg aaatggtata aagagtattt ggaatccgga gagctccatc aggagcaccg
     1081 aaaaaaatca aagtattctg aagaacaaaa acgaatagcg gtgaatcatt attttgaata
     1141 tggtcaatgt tatgcacgaa ccattcgcat gcttggttat cctaatagag agtccttgcg
     1201 gcagcggtgt gaagaacttg caccaggagc tagaaaactc cgaaaaagtg cggtaaagtt
     1261 aacacaagat caaaaagaag ctgtgttgaa aaaattctat aaaccgcaaa ctaataggaa
     1321 aaatttagcc gaatctggag gaattagtag agttactcta tatcaatgga aaaacacggt
     1381 tcttggaaag gattttccat tgtgtatgac tttaaaaaat caggaaaaac aaaaagagct
     1441 acttttaaag gaagtagaag acttacaaaa acaagtacac caattacagc tcgaaaaagc
     1501 attactcgaa ggagcggctg aactgctaaa aaaagaaaaa ggcgtcaatc ttctttgtct
     1561 atcaaatcaa gaaaaaacga tattgattga tgccttacga aatcagttca ccttgaaaga
     1621 gcttcttcaa cagctccagt ttcctaaaag cagctatttt tatcaaaaac aagcacttga
     1681 gaaaccagat aagtattaca aagaacgtca aattaattat cacgattttc aatcataact
     1741 tctgtgcgta tggttaccgt agaattcacc aagctttaaa aaatatgggt aaaaagcttt
     1801 ctgaaaaagt tgtacgtaga ttgatgactg aggaaaatct atttgttaaa ttttctagac
     1861 gaaaaaagta ttcttcgtat gctggggaaa tttctcctgc acaccccaat ttattaaatc
     1921 ggaattttaa agcaaagaag ccaaatgaaa aatggttaac agatattact gaatttagaa
     1981 ttctcgcagg gaaaatctac ttatcaccac ttgtcgattg ttacgatggt gctattgtta
     2041 gttggactat tggaacaaag ccaacttgct tcactctatg tctaaaaaga gttattcacc
     2101 agataattca gcgtgcgaag gtttctttgg acgattaaaa aacgagatgt tttatcaacg
     2161 tgactggaag aatacaacaa tcaatcagtt tataaacaaa ttggatgatt atattcattg
     2221 gtataataat caacgaatta agttatcttt aggaaggcta agccctcttc aatatcggaa
     2281 gaagcaaaga tatatttgtt aaaaaggtcc aaaaaaaata tccgcacccc cttttttttg
     2341 taaaaaacta ttgcacaata caagtagcct acggctatcg aaatttccat aactttcgag
     2401 caagaa
//
LOCUS       DACDUU010000149         2389 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.152, whole genome shotgun sequence.
ACCESSION   DACDUU010000149 DACDUU010000000
VERSION     DACDUU010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.152"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            206..2068
                     /locus_tag="IWL30_002856"
     CDS             206..2068
                     /locus_tag="IWL30_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9707547.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2068..>2389
                     /locus_tag="IWL30_002857"
     CDS             2068..>2389
                     /locus_tag="IWL30_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9707548.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETA"
BASE COUNT          858 a          353 c          466 g          712 t
ORIGIN      
        1 aaaatttgaa aaagcgtttg attatttata ttacgactta gtgcaataaa aaacgctttt
       61 taacagtaca tgtagtcgtc tttataatag ataaactctg taatattagt taaaaagcta
      121 taagttgcac ttagttaagt gcaaacattt catattgtcg ttagtttaga aaacgcttat
      181 attagcaatg taaggagaga tgataatgca gcagctaaca aaaagagaaa ggcagctttt
      241 gatttacttg gtggaaatgt ctgaaccggt aacaatcaaa aagttggctg aaaaagaaaa
      301 tgttagtgtt cggacgataa aatacgatct agatgatata agagagtggc tgaatgagcg
      361 gaagcaagtc ctatatagca aacgtagtca gggaatttgg atgaatatta gtagctctga
      421 tcgaatcaaa ttaaaaagcg aattgatgga tgttaatcgt tttgaaatat ttgcagatca
      481 aagtctccga atcaaacgtt tactaattat gcttttagta acaaaagaag ctattactgg
      541 aatgaaaatg gccaacgcat tagatgtcag caaagatacg atcatgaatg atttagatgt
      601 gctggaaaat aattttctta aggaaggatt gaccttgaat cgtcaggcgc gaaagggttt
      661 ttggatcata ggagaagaac gtctagttcg attaacgatt gaagagattc ttcaaaaaga
      721 gttcacggat tatgacattt ataaattaat gtcattgtta ttgaatggcg gtgagactga
      781 atactttgaa atgtattctg ctactgctac acctatccaa gaggtattta atcaagtaat
      841 cattcgaatg cgacacttac tggaatttga aaatctagag aaactgaatt acgcggaatt
      901 actaaatatt ctgattcgag tgacaattgc taccgttcgc ttacgcaaag aggctacgat
      961 tggtcgttat caattggttg ctgaacaaga attagacaaa aacgatcttt cttatcgcgt
     1021 catgcagcag gtctttgatt attatcagtt gccactgttc gaggatgaat atcattatat
     1081 ctatagtgat acattcgaca cgattcacca gcaagacgtc atgaaactag ttgagaatgt
     1141 gatttataaa gtaagtaaag caatcaacca ttctttttat caagatccac aattgttgac
     1201 gaatctgtat gctcatttat ccatgcgatt atcgagaaag aaaaaatttg tcaatgaata
     1261 taatcctttt aaagaagata tcaagcagaa atacccggaa ttatttgatg cgatcaagca
     1321 agcagttcga caagaaataa gtgaacaaaa tattctgatc aatgattctt tcgtggcata
     1381 tattgcctta cactttttgg tttcctacga aagagaagaa gatctacgaa gcgttagagc
     1441 cgtctacatt tgttcaacag ggttgggtgt gactagtctg atcaagcaaa agatcaatga
     1501 agaattaaat aatatcacta tcgtggcatt tgcttcagta ttgaatgcgc aagaaatgat
     1561 tgctaccaaa gatcctgatt tggtgattag tatttttcca attaatggca tcgatcgtcc
     1621 tttcatcaaa gtccatccat tacccactga acaggacttg aaaaaaattc aacaaataat
     1681 aaaaaaaata cttgcttctt ccaatcagca ggaaaggcat aacttgaatc ttagagaaac
     1741 gtccatcaat aaagatactt tagaagactt tagcaaggaa ttgattttga aagcttatac
     1801 gatttatgaa cgtttacttg tcattttgga agcagaattg atccaagaat atcgagaagc
     1861 ttttttgctt catgtattct taatggttca tcgtatcaca tttgatcaac aatatatggc
     1921 agaaggtcgt caagtagacc agattatttt agacaaagga acactggtag gaaaaatcga
     1981 agcagttttt gcagaaaata atttaggaat caatcagtca gaaatcagtg cattattctc
     2041 atatataaaa gaaggagcgt caagttagtg aaaatgtcag acgaggcttt aaaaattatc
     2101 gaggaaagtc aaaatcggga aaagttaaaa caagtgattg atctaattga agagcgatta
     2161 gtagaacatg ctattgtacc aaccgagttg cagtggacca ttctaattaa tcacttgaat
     2221 gagatgctga aacgttctca aagaaaggaa acgattcccg tagtcgatcg ggatttgttc
     2281 aaagaagtat ccaaagaagc cataactatt tctgatgaaa tagttagaag tatcggtaat
     2341 ttaaccgatg atgaaatata tgttttgtcg attcattttg aaacagcaa
//
LOCUS       DACDUU010000150         2351 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.153, whole genome shotgun sequence.
ACCESSION   DACDUU010000150 DACDUU010000000
VERSION     DACDUU010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.153"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..544
                     /locus_tag="IWL30_002858"
     CDS             <1..544
                     /locus_tag="IWL30_002858"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9707549.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(1017..1589)
                     /locus_tag="IWL30_002859"
     CDS             complement(1017..1589)
                     /locus_tag="IWL30_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9707550.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1605..2210)
                     /locus_tag="IWL30_002860"
     CDS             complement(1605..2210)
                     /locus_tag="IWL30_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAP9707551.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          741 a          468 c          359 g          783 t
ORIGIN      
        1 catttcaaaa gagaatctct taaatcagga cttttctact gaaacgatct gtgagaaatg
       61 ggcagctgac attacgtaca ttcctactaa gaaaaatggt tggtgttact tatcttcaat
      121 catggattta cacacgaaaa gaattattag ttatacattt tcaaaacgaa tgactgtgga
      181 ttgtgtcatt caaacattga ataaagcaaa aatacattat cacattccag aaggaatgat
      241 tctacacact gacttgggca gccaatacac agcaagagaa gtggaacaat ggcttaaaac
      301 caacaaaata aggcattctt atagtagaaa aggaacacct tatgataatg ctggaatcga
      361 atctttccat gcctcattga aaaaagaaga agtctacacg actagctact cagattttga
      421 agaagcaaat cgagcgctat ttagctacat tgaaggattt tataaccgaa atcgaatcca
      481 tagctcgatt cactatctaa ccccacagga atttgaagaa ttagcaaaag aaaaaatggc
      541 ttaaccgtct ctactaaaat gtgtccaaga tattgactca aatccagtaa tatcacaata
      601 ggaatgctgt ttattatatt agcagcagtg ctgattttta tgatagttat ttggttctta
      661 aatgaaagat ctaaagacaa tcaacaccgt agggatatgg agataaaatc aatagagtca
      721 atagattatt tatcgataat aacactaaaa aagccacgtt cctaaacgtg acttacgctg
      781 aacgatatgt gttgtttggt agacgaacaa atcgttcatt ataaatatat gatggtatcc
      841 taatatagta agcgtggcta gcataccttt aattacttta ccttttacag ttaccataca
      901 cattttttga gcagaaatca agtaaataaa aaaatattat atttcacaac atgctaatag
      961 actcatatct agaacgcatt aagtatttac ctgtctggtt cgctgagact tcttttttaa
     1021 ctttcttcaa ttttcttctt tattctaaaa agcgtactgc gactgaaacc ggttatagct
     1081 tcaacttcct tatacgattt acctgaagta agcagttcat atgcgtgcct cttcttcggt
     1141 gtgattgttg ctttcggacg tccttctctg aaattaggat tattctttct tgcaaacatt
     1201 tttccttctt gggtacgttc cacaatcata tcacgttcca tttctgctac actcaacaac
     1261 gtccgtgtca ccattcgccc catggacgta ttttcaatcg ttcctagatt caaaaccttg
     1321 atcgttacgt tatcatcgag taaaggttca atgatctcta atgcttctct cgtgttacga
     1381 gcaaatcgat caagcttcgt aacaactatt tcatctccag gcttcagacc agccaacagc
     1441 tgattgaatt ttggccgatc ccgttttgta cccgtaaatt tttctgaaaa aatattcttc
     1501 ttctctacgc agttctcaag caactgttga atttgttctt ttgtactttg agaagcggtt
     1561 gagacacgtg catatccata tttcgtcatt tggtccacct acttttacaa atcatctatc
     1621 gtatactcag aatacccttc atagaaatag ctgacatcga ttcctttcat atataactcg
     1681 cgatcgtcaa ttttatcagt cagagcattg gaaattagat agcgtatttc caaatcgttt
     1741 atgggacttc gctccattgc agaaagatag tctgctttat cgattaagtt ccaatctaca
     1801 actttttgca attcttcctt taaaatgaga tccagccaaa ttctagtact tcggccattt
     1861 ccttctcgaa atgggtgagc gatattcatc tccacatatt tcttgataat ctcttcaaaa
     1921 gaagtttgtg gcatctgatc aatatgctgt aacgaatgtt ctaaatacat aactggtgca
     1981 aatcgaaaat ttccttttgc aatattgacc gatcggattt ttccggcaaa atcgtaaata
     2041 tccgaaaata gaaattcatg aatttcagat aaacctttaa atgtaccaac ttcgatttca
     2101 tttatttttc cacaatcgta caattctttc gctttttgtt tactgagttt ttcttcaacc
     2161 ttagccaact caacttgatt ggttaatccc aatttattct ctaaaatcat gcgaaatctc
     2221 cttaacttta tgacataagt ttatgaaaca ctctcaaccc cattatatcg cgattttgtt
     2281 tatgtgtcaa aaactattaa gtttctgacc cccgatagat ttttcaagtg tctctatttt
     2341 gcacagtcta g
//
LOCUS       DACDUU010000151         2325 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.154, whole genome shotgun sequence.
ACCESSION   DACDUU010000151 DACDUU010000000
VERSION     DACDUU010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.154"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            40..423
                     /locus_tag="IWL30_002861"
     CDS             40..423
                     /locus_tag="IWL30_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707552.1"
                     /translation="MLVSVPYTDQPPLEGIRADGEPLTIADQMFDPKLNQWIILANAL
                     DHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIKQENTALKEKADSLAQINSKMM
                     LASLQNSKDISEIKEQLNPASKGGE"
     gene            425..562
                     /locus_tag="IWL30_002862"
     CDS             425..562
                     /locus_tag="IWL30_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP9707553.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            600..893
                     /locus_tag="IWL30_002863"
     CDS             600..893
                     /locus_tag="IWL30_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707554.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            890..1114
                     /locus_tag="IWL30_002864"
     CDS             890..1114
                     /locus_tag="IWL30_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP9707555.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            1111..2136
                     /locus_tag="IWL30_002865"
     CDS             1111..2136
                     /locus_tag="IWL30_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9707556.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
BASE COUNT          803 a          385 c          479 g          658 t
ORIGIN      
        1 cgtactggcg gagagtacgc aggaaaagta ttggacgaca tgcttgtatc cgttccttac
       61 acagatcagc caccgcttga aggaattcgt gctgatggcg aaccgttaac aatcgctgat
      121 caaatgtttg atcctaaatt gaatcaatgg attattttag cgaacgcact agatcacaac
      181 gatttaaaca atctcaaagc aatgtatgag tcgttagaaa atgagaacgg cgatttaaaa
      241 cagatcaatg ccaaactcat gctaagcgat gtagcaatta aacaggaaaa tactgcattg
      301 aaagaaaaag cggatagttt agcacaaatc aattcaaaaa tgatgcttgc ttcgttacaa
      361 aatagcaaag acatttcaga aattaaagag caactaaatc cagcttcaaa gggaggtgag
      421 tagtatgttt agttttagcg atgtgaaaat gatgtatgat tggggctgtt tcactaacga
      481 acaagtaatg gttttcgttc cgttgtgcat tactgaagaa aaagcagata aaatcattag
      541 caaagaagag agcgcatctt aattgatgtg ctttttattt tgattcaagg agttgtcaca
      601 tgattaattt aggggaatgg ggagcgatag caggatcaat aaccgctatc gtttctttga
      661 ttttattagt aataaaacca attactgcat ctttctcgaa gattactgag actctttcaa
      721 aagtaagtca caatttagat ttgctgacta aagatttaga atcgagcaaa tcagatcgat
      781 tgatgattca tgaagaacta aagaaacacg atgaaagatt agatacacat gcagaaaaat
      841 tggtagaaca cacgcaacaa attaaaactt tatttaggga gagaagaaaa tgaataataa
      901 aacgttcgaa gtactaaaat ggttcgcact ggtaattatt cccgcactag ctactttcgt
      961 ggggttagtt ggtaaagcgc tcaattggca gtacacagat atctgtgttg tcatcattac
     1021 tggttttggc gcgtttttag ggagtgtgtt gggtgtatca aatcgaacct acaaaatgtt
     1081 ctcggctgaa agcgaagaag gaggaaacaa atgaaaaaga aaattactat tactgcgatg
     1141 agcctgttaa cggctctttt tttattaccc attaatacgt ttgcttatac tattaatgac
     1201 gagtataatt tagcgccgaa tcaaggagac tccagattag caattcctaa caaaattatt
     1261 ttgcacgaaa ctggaataga tgcaccagca agaaacgtag ccgccaacat gaaaaataat
     1321 tataacggaa gcaattctta tactacagat gttattggtg acggtgggat tgtttaccgt
     1381 gtgggtgagc aaggatatgt ttcgtgggga gctggtaacg ctaatcctta tgcgcctgta
     1441 cagattgaat tacagcgcac atatgataaa gcattgtttg aaaaaaacta tcgagcttat
     1501 attgaatata caagagatag tgcaaaaaaa tatggaattc cattgactct tgatcaagga
     1561 acttctttat ttacaaaagg aatcatttct catttgtggg tgacaaatta tgtttggggg
     1621 aaccacacag atccatatgg ttacttatcg caaatgggag ttagcaaaga aaagcttgct
     1681 tatgatttag ctcatggatt taccgatgaa aatccaacaa cttctgaaaa caagcctgtc
     1741 attgatccaa cacgagctgg tgcagctaat cctacgctga cagatggaac aaatcacgcc
     1801 cacattgatc agtttgggga aatcgaaaac gcaaacttgc atgtggctgg atggcacatt
     1861 gctaactata aatacgagta tattttcatt atggactaca atactgggaa agaattagct
     1921 cgagtaagag ctgatggaat ttatagacca gatgtaaacc aagcttataa tacttctgga
     1981 aatgttggtt atcatgtatc tttcaatatg cgtaattttc ctagtaagaa agtctatgta
     2041 atgatgcggg caacgaatga tccagaggga aacactaaag gcggtgcgca agatttccat
     2101 gacaaacgtt ggtatttaaa tattcctaaa cgataaaaat agctcctcgt tgaggagcag
     2161 tacataacta tattgacaac tataaaaatt attcgataaa atagtgatgt tatcgcatat
     2221 cttcactatc acccataaat agtcacactc caagctatgc gataacaggt ttgttgccac
     2281 acattctact ggttgattgt ttatggcttt atgtggcaac aacca
//
LOCUS       DACDUU010000152         2249 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.155, whole genome shotgun sequence.
ACCESSION   DACDUU010000152 DACDUU010000000
VERSION     DACDUU010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.155"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(148..2145)
                     /locus_tag="IWL30_002866"
     CDS             complement(148..2145)
                     /locus_tag="IWL30_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAP9707557.1"
                     /translation="MIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKA
                     LVTNKANQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHL
                     KEATKRLSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVY
                     IKNSGVTDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADE
                     AEKYLAKGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGS
                     NLMLSMNAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHE
                     QGSKEITENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKS
                     SVFNRSGQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRD
                     SFKEFGLGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYT
                     PIQLAQYASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMA
                     ILREGFHQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGP
                     TDNPEIAISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          572 a          504 c          411 g          762 t
ORIGIN      
        1 ttttgtcaca accttttttg ccgagtaaac ggtgttcaaa agccagtttc tcggtatgaa
       61 gtcaaattta agcaaaaatg tgagaagcta ttttcacaaa atttttcttc atactcgaaa
      121 ctaagcggct ttttcacgac ctctttttta ttttgccatg aacatatcat aatacgcgtc
      181 cattatttct ttagcaatcg tcaagtttat gtggtcttgt tcgtctaata agttcggcaa
      241 gacaacactg atggcaattt ccggattgtc tgttggtccg tatgccacga tattactatt
      301 gactgtcgca atatctggat gaccttccgc aaccgtttct gcagtccctg tttttgcgga
      361 aaggtccatt ttcgcactgg ctaatggttt ggctgttgtg taaggatcgg taccatggac
      421 aacttggtgg aatccttcac ggaggatcgc catattttct gcagaaatat tgaccgtatt
      481 ttctactgtt gtgccaattt ctttcttcac ttcacccaag tttccatttt cgtcattgcc
      541 atagatcgct ttgactaggt gcggctgtac tcgtttcccg ccattagcga ctgttgaagc
      601 atattgagct aattggattg gtgtatatgt gtcaaactgt ccaaatgcca aatcggtaaa
      661 gttacctgga gcataccatt ctttgccatt atccgcattg aacttcttca tataatcaac
      721 agaacgtgag atcccaggag attcattcgg caaatcgatc ccagtcgttg taccaagacc
      781 aaactctttg aaagagtctc tcaattgctg ataagcttta gcttcttcat ctaaactagg
      841 tagtcccatt ccaggtgtat agtctagtcc taacatcttt aacgccactt tgatcatata
      901 cgtattagaa gacaattccg atgctttgac tgcatcaaga gcaacttgtc cgcttcgatt
      961 aaagacagag cttttttctg aagcaccttg cagacgaatc ggctcatcaa tcaaaacttg
     1021 atttccgctg atcgcgttac ttgcccagcc tgctgtcaaa gtaccagcct tgactacaga
     1081 ccctggatca aaggcgctag taatcgtgcc taaagcattc tcggtgattt ctttggaacc
     1141 ttgttcatga ctgaaaccag tcattgctag cacttctcct gtctgaggat tcatagccac
     1201 tgcataagca ccaggcgaat actgtgcttt gccgttgttg atcaatgttt gataattcct
     1261 tttcaaaatt tcttctactt ttgattggaa ttctgcattc atagataaca ttaaattaga
     1321 acctttttcc ccagagaaga tttctttttg attcgaaaca tttccttcat tatcaaggga
     1381 aacttcatat tgggttttgg ttccttgcag gacatcttca tattgttttt ccaaataact
     1441 ttgtcctaca cggtcattcc gagaataacc ttttgctagg tatttctctg cttcatctgc
     1501 tggaagtcct tgcttttcag tcgtcacact gccaagaata cttttcagtg aacttgctgc
     1561 attatattct cgtgtccagt ctgtgccagt cgaaactcct ggaagttcag aagcgcgttc
     1621 tgccacaaca gctaactctt gatcagtaac accagagttt ttgatataga cagtgtttaa
     1681 ttcatatgca ctattcatcc gtttaaagat ggtagcaatt tttaattgct cctcgtttaa
     1741 attaatctca tcagctgtga ctttattgac catcgcacta tattgttcgc tgttccctaa
     1801 ttgcttttct tttgccgaaa gtctttttgt ggcttctttc aaatgtttgg gatcagccag
     1861 ccaaaaatct tttttatctc tatctgtcag attttcatct accggaacat cgatcagcga
     1921 gttcagcttc gttgcgattt caagcaaatc tttagccgta acttgtgttc ctctcgtata
     1981 tgtgatggcc tgattcgctt tattcgtaac taaagctttt cctgtggagt cgtagatcat
     2041 tccgcgagga gtactttctt ggaccgtaat cgtagaagta gacttcactc gcgcctccat
     2101 ctcttcgccg ttcacgatct gcaaataacc caatctagca atcaacgcaa caaataacgt
     2161 aaatataatg aaaaaaagaa aatttaagcg aaaaggaaca tgggaccggc gaaacgtttt
     2221 gttcttaggt tcttgtgcac cttttctta
//
LOCUS       DACDUU010000153         2192 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.156, whole genome shotgun sequence.
ACCESSION   DACDUU010000153 DACDUU010000000
VERSION     DACDUU010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.156"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            394..1821
                     /locus_tag="IWL30_002867"
     CDS             394..1821
                     /locus_tag="IWL30_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HAP9707558.1"
                     /translation="MMRKQSIEGRNQFAMLTIDDLVPKDHLVRKIDAAIQFDFIYPIV
                     ESTYSTYGRPSIDPVVLIKLVFIQYLFGIRSMRQTIKDVETNVAYRWFLGYSFEDPIP
                     HFSTFGKNYVRRFRETTLFEDIFSHILEQAVKAGFVTEDNLYIDSTHIKANANKHKFT
                     KEMTYGQAKAYQDELEDEINKERIAVGKRPFTWDTESELSLQKISHSDPESGYYVKGE
                     REKQFAYSAHTSCEDNGFILSTLITPGNVHDSQVAIDLVKQSKRAFPNIKRVVADAGY
                     KTPKFVHFLSHLKLYPILPYTAPHGVKGMFRKKDFVYDAYFDCYLCPNNQPLLFSTIT
                     RDGYRTYKSNPKFCVSCPLLENCTLSTKHQKIVTRHIWADLMDELEHQRHTALNREIY
                     KKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLTFAAMNLKKLATWLWKTKKPM
                     AFYFKIMQRLTKRSGLITNSFLSTI"
     gene            complement(1852..2115)
                     /locus_tag="IWL30_002868"
     CDS             complement(1852..2115)
                     /locus_tag="IWL30_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circular bacteriocin, circularin A/uberolysin
                     family"
                     /protein_id="HAP9707559.1"
                     /translation="MKKNLLLVLPILGFAGFFVGVPMLSANIGISSYAAKKVIDIINT
                     GSTVATIISIVAAVVGGGLITAGIVATAKSLIKKYGAKYAAAW"
BASE COUNT          767 a          377 c          361 g          687 t
ORIGIN      
        1 cttccttttt tgtatggatt ttttcattta cacaaaatat tttatactct cctgaatttt
       61 ttcatgttga aaacatcact aatcaaaggc aaattataat ttagaaatag aatgaaaaca
      121 ctaaatgaaa atagaacatt agaaaaaata gaatcaaaaa aacccaagac tagtgccgaa
      181 ataagggaaa caatcgaagc tacaaaaaat aatgatttcg ttgaacagga ccaatttatt
      241 tgtttccgat atttctcaaa aagaactttc tttgggaaag ttaaaggcga ctgtaaagtc
      301 gcctcagact gtagacaaac tcctaaattt ggagtgttgt ttgcagtttt ttgttttata
      361 ctaattgtat aaactattct gggagtgatt ctaatgatga gaaaacaatc gattgaaggt
      421 agaaatcaat ttgctatgtt aacaattgat gatcttgtac ctaaagatca cttagttcga
      481 aaaattgatg ctgcgattca gtttgatttc atttatccca ttgtagaatc tacgtattca
      541 acctatggtc gtcccagtat tgatccagtg gtcttaatta aattagtatt tatccaatat
      601 ttatttggta tccgttcgat gagacagaca attaaagacg ttgaaacaaa tgttgcttac
      661 cgttggtttt taggttattc attcgaggat cctatcccac acttctctac ttttggtaaa
      721 aactatgttc gaagatttag agaaacaact ttatttgaag atattttctc tcatattcta
      781 gaacaagctg taaaagctgg cttcgttacg gaagacaacc tttatattga ttctactcat
      841 atcaaagcaa atgccaataa acataagttc acgaaagaga tgacctacgg tcaagcaaaa
      901 gcttatcaag atgaattaga agatgaaata aataaagaac gtattgctgt aggaaaacgt
      961 ccttttacat gggacacaga aagtgagttg agtcttcaaa aaataagtca ttcagatcca
     1021 gaaagtggtt actatgttaa aggtgaacga gaaaaacaat ttgcttattc tgcacatact
     1081 tcctgtgaag ataacggttt tatcttatct acattaatca caccaggaaa tgtgcatgac
     1141 agccaagtcg caatcgatct ggttaaacag tcgaaaagag cttttccaaa tataaagcgt
     1201 gtggttgctg atgcaggata taagacgcct aaatttgttc attttctgtc acacttgaaa
     1261 ctctacccga ttcttccata tactgcacct catggagtga aaggaatgtt tcgtaaaaaa
     1321 gactttgttt atgatgccta ttttgactgc tatttatgtc ccaataatca accgttgtta
     1381 ttttcaacta tcactcgtga tggttatcga acctataaat caaatccaaa attctgtgtg
     1441 agttgtccat tacttgaaaa ttgtactttg agtacaaaac atcaaaaaat agtgactcga
     1501 catatttggg ctgatcttat ggatgaactt gaacaccaac gccatacggc gttgaataga
     1561 gaaatttaca aaaagagaaa acaaaccatt gaacgaatat ttgcagatgc aaaagaaaag
     1621 catggtatgc gttggacgaa atatcgaggt cttgaaaaag tcgccacgca cacgatgctt
     1681 acgtttgctg ccatgaattt aaaaaaacta gccacatggc tatggaaaac aaaaaagcct
     1741 atggcttttt atttcaaaat tatgcaaaga ctaacaaaaa ggagtggctt aattaccaac
     1801 tcctttttgt ctacaatctg aggcgactgt aaagtcgcct ttagtccttt tttaccaagc
     1861 tgctgcatat ttagctccat attttttaat taatgatttg gctgtagcaa caataccagc
     1921 tgtaattaat ccgcctccaa caactgcagc aacaatagaa atgattgtag caaccgtgct
     1981 accagtatta attatgtcaa taactttttt agctgcataa gaagaaattc caatgttagc
     2041 acttagcatt ggtacaccaa caaagaatcc cgcaaaacct aaaattggta aaactaataa
     2101 taaattcttt ttcataataa ttcctctatt tttttatttc gccgtttgta taattaagcg
     2161 agacaaataa aaaagccatt tatgttacac tc
//
LOCUS       DACDUU010000154         2088 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.157, whole genome shotgun sequence.
ACCESSION   DACDUU010000154 DACDUU010000000
VERSION     DACDUU010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.157"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..296
                     /locus_tag="IWL30_002869"
     CDS             <1..296
                     /locus_tag="IWL30_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP9707560.1"
                     /translation="ATYILRYDDKIIEIHLDYFGRVPTRKFQLFTLDECINVDLLNNK
                     ISFEENIVKEMPESANDMYEKELKYFFENVVTGKENWNDLYHANEVLKIAEEK"
     gene            297..1049
                     /locus_tag="IWL30_002870"
     CDS             297..1049
                     /locus_tag="IWL30_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP9707561.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            1054..2001
                     /locus_tag="IWL30_002871"
     CDS             1054..2001
                     /locus_tag="IWL30_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP9707562.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          737 a          274 c          408 g          669 t
ORIGIN      
        1 tagcaacata tatattgaga tacgatgata aaattataga aattcattta gattattttg
       61 gaagagttcc tacaagaaaa tttcaattgt ttactttaga tgaatgtatc aatgttgact
      121 tattaaataa taaaataagt tttgaagaaa acattgttaa agaaatgcct gaatctgcta
      181 atgatatgta tgagaaggag ttgaaatatt tctttgaaaa cgtagtaaca ggcaaagaaa
      241 actggaatga tttatatcat gcaaacgaag tattgaaaat agctgaggag aaataaatgg
      301 aaattttatt tactatttgt ggtagagctg ggtctaaggg gtttaaaaat aaaaatttga
      361 aatatttggc aggaaagccc ctagtccatt tcgctttagc aatgatagat atctatcaac
      421 aaaaacatcc tgaacataat tgcacgactt gtttgaatac ggacagtaat gagttagtag
      481 aattgacgac taagttaaat ccgtctgtga aaattgtaga acgtaaacct gaattagcta
      541 cagatactgt agggaaaatt gaagtgatac gtgattcatt cttacaacta gaaagagaag
      601 ataatatatt tgatttaatt atagatttag atttgacttc acctttacgt gaagtttcta
      661 atcttgaaga attaatagat gaacatctaa aaaatttaga taaagacgtc tacttttcag
      721 ttgtttctgc aagacgtaat ccctatttta atatggtaaa aagaattgaa aatgaagtta
      781 ctatagttaa taaatcaaga tacacatcta ggcaacagac acctgaagtt tttgaaatga
      841 atgcctcaat gtattctttt aaacctgaat ttctaaaaaa aagtaattat atttttgatg
      901 ggcaatgtgg gataataaaa atgagagatt atctaatttt agatattgat agtgaagaag
      961 attttaaatg gttagaattt ttgtttccta aatttttaga agattcttcg gagcttcaag
     1021 atatctataa ttatttatat cggaggtaaa aggatgagct gttatcttat caccggtggg
     1081 gctggcttca ttggttctac cttagccaat catttaggag aagaacatgt ggtaatcgtc
     1141 gtcgatgact tatcaatggg aaaagtagaa aatcttgata tgacgaaaaa tatcactttt
     1201 attgagggag atgtcgctga gaaatccttg atggaagaga tcatgaaagc taataaattt
     1261 gattatatct ttcatttagc agcagttgct agtgtggcag attcagttgc ccgaccagta
     1321 gaaacacatc gtgtgaattt tgaaagtgta ttaatgattt tagaattgat ccgcaagtat
     1381 caacctgatt taaaacggat cgtgtttagt tcttcagcgg ctgtttatgg ggatgaaccg
     1441 actttaccaa aaaaagaaga gtccgtgatt cgcccattga cgccttatgc ggttgataaa
     1501 ttcgctgcag aacaatatgt gctagattat tgtcatttat atgatgtacc cggtagcgca
     1561 gttcgttttt tcaatgtcta tggtccaaat caaaatccga attctccata cagtggtgtg
     1621 atttctattc tcgtagatcg ctataagaaa caaatcgtag gagaagcaac ttcatttaca
     1681 ttatttggtg atggaagcca gtctagagac tttgtttata tagatgatgt aatccaagca
     1741 ttacttttag ttgcgaatga ggaagctgca ctggggaaac aattcaatgt tggaacgggt
     1801 aaagcaacca cacttttgga attgattcat gcaatcaatg aaattctcgg tgtagaattg
     1861 acacttgaat acaaagaaga gcgttcgggt gatatccatg actctctagc agatattact
     1921 aagatccagt caattggcta tcaaccgaaa tatggtattt taagtggaat gactaattat
     1981 ttgaaaactg aaatcaatta aaacatacta agataagatt agagggtaaa ttcaggctct
     2041 ttgtcaataa ggactgatga gttttgtaaa atcaaatctg ggatagaa
//
LOCUS       DACDUU010000155         2032 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.158, whole genome shotgun sequence.
ACCESSION   DACDUU010000155 DACDUU010000000
VERSION     DACDUU010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.158"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(486..1460)
                     /locus_tag="IWL30_002872"
     CDS             complement(486..1460)
                     /locus_tag="IWL30_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP9707563.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTITPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
     gene            1623..1880
                     /locus_tag="IWL30_002873"
     CDS             1623..1880
                     /locus_tag="IWL30_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707564.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
BASE COUNT          758 a          313 c          266 g          695 t
ORIGIN      
        1 tactatttgt aatctatagt atcagtccta taaaataaac atctaattaa ttactctaaa
       61 tcctaattat aagccttttt tctcactttt tttcaaaaat atggtatgct tcttagtgaa
      121 ttcaaatata tggtatgctt cttagtgaat tcaaatataa aagagtttaa agcgtaatac
      181 acttatgggg aagtggtttg gggtgcgctt taaactcttc tttattatta tctcacagca
      241 tgcttaaaag gtctatggat taagttatgt aaaaaagtgc ctctttaaac gtcctaaaat
      301 ccaaacaagg atagcataat atacactatc gttgatccag aaaacaggta aataaatttt
      361 gcaaaagcaa taagttaaaa acattacaaa aaagacaagg atatcacatc aagaaaaaat
      421 gttttgaaaa tatctttgcc tttttgtatt atatctatat taaataatca atcacaacac
      481 attaactaat ttatatattt aatttgttgt ttttctataa ttggataact aattaactta
      541 tgactatcta agtcttcttt attcatatca attgcagtaa tttgactgtc ttcatatgtt
      601 cgataataat agattccttt gtcaacattg caacaagaag aataaattgt atattcatat
      661 tttccatcac caacatcaca caaacctttt tgttgttcta ctgaacctaa gatatggaaa
      721 aattgactaa tactttctga ctctgaatct ccagatacag aattcagctt cgtaaaagtc
      781 gctttaacaa aacgagatac tgaggataaa tctccaggca agcctatccc tcccataccg
      841 cggctatagg cattcaaact tatttgattt gaaaaattat ttttaggagt ttcactcgat
      901 aagacacgat aattgtttaa attaaataat tgatagtcaa atgaaggatt attggtaaga
      961 acgcccacag ggttatcata tatatgaagt ccatctttca tactttcaat gacaattgat
     1021 ttttctttat cagctaatag ccaatgtaaa ggggataaag gaagttcgtc actataattt
     1081 atatttgcta aattgatatt ttttaacaat tttttagctt ctcctactgt tgagcattgt
     1141 cctaaaatcc aaggaataaa ttcaaaagga gatacattgt ctttcccttc ttgtatttct
     1201 ttataatcag catacccaga aaaatttagt ccagccatac tcaatccttt ttcatttgtc
     1261 gcatcgtaat aaagagggta gtcagctata ccagcggcaa taccaatcat tgcataatga
     1321 gtatccaaat catttacctt tcgaaaattc aacttataat ttcttggagt aatagtgact
     1381 acttcattgt aagatatttc ataatcaaaa ttccttccaa aataatgatc acttgttaca
     1441 taagtaatag acgtacacat agtttattcc tcctaaatat ttttctgtcc acgttcatat
     1501 tactccaaag ttttcctcat tccaaaagaa aagcacagaa tacacaaaga agcgttttta
     1561 tttgtataaa ctatatcaaa gttctatact taaattatca aataaaaagg acgtgaagat
     1621 aaatgtcaaa tttagaaaac aaagaagaaa aagttgtaaa caaaattgtt tctgttgtca
     1681 ataaactgga taaagagtta gacgaacttg atacgttatc tgaaaatcca gaaaaaaaac
     1741 ataacctaaa aaaatggtta gttgaacgaa aggctattca tgaaattaaa aaagttcttc
     1801 atgaagctga taaatatgaa aaatatgatg aaaaagaact tgataaagaa tttaaagaaa
     1861 tcaatgactt attactataa ttgatttata cgactcattt ttgagtcgtc agactgcaaa
     1921 caacactcta aatttaggag tttgtctaca gtctgaataa attttaaatt tattgtccat
     1981 aatgttatta tgaatttata gcattaagaa aagagataag actttacatt tt
//
LOCUS       DACDUU010000156         2021 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.159, whole genome shotgun sequence.
ACCESSION   DACDUU010000156 DACDUU010000000
VERSION     DACDUU010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.159"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            101..571
                     /locus_tag="IWL30_002874"
     CDS             101..571
                     /locus_tag="IWL30_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAP9707565.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            793..1896
                     /locus_tag="IWL30_002875"
     CDS             793..1896
                     /locus_tag="IWL30_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP9707566.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT          687 a          328 c          460 g          546 t
ORIGIN      
        1 agaatctctg aagaataaat cacgtgatcg tgacgataaa atacacttac gtcaggtgat
       61 aaaaaataca taagtagaaa actaagagga ggaacagaaa atgaaagaag ttcaattgat
      121 cagaaaaagc gaactatcag aaggtggttg caatgcatgc ggcgtagtag aagcaacaag
      181 ctatacatta aagcttggtg ccaacaaagc gataatctct gagttgacag taggcggtct
      241 tgtggacagt ctggcgcttg ctgaaggttt tattggagaa gatatttatg agatgttttc
      301 agaaatccgt cagttaaaaa aaggggaaaa ctgcatagag gttcatcatg agagcccgaa
      361 tgtgagattc aaacgtggtg ataatgagat gatcttcaat aatcatgtgt ccgatcacac
      421 cgagctatat ggaatcgtga atcaaatact aactgagctt tttggattgg gaccttatgc
      481 gttcaaggaa gaaaacggta atccaaaatt aaacgaagaa tggcaagaaa cgattgaaat
      541 acaaagaaac aacccgcact tatttcaata ggcatccaca gtatttcgta aaagggactt
      601 tgacacgact tttgtcaaaa tcccatcgtt ttttatctat ttgtgttcta aacatttctt
      661 tcagagtagt aacaactgga atgactgaaa tgtaaacaga ttatttgcct gtatcaatac
      721 ggaagagtaa tataaagtgt gtaaacgcta tcatattatt cattcaattt tttatagagg
      781 gaaggcattg aaatggaaaa aacgtatcaa gcaggtacaa atgaaggaat agtggatttt
      841 atcaatatgg aagatttaga aatagccgct tcacaagtga ttccagctgg tggttatggt
      901 tacatcagca gtggtgctgg cgacttattt acttatcaag aaaatgaacg ggcattcaat
      961 catcagctga ttattcctca tgtattaaga gatgtagaat tacccgatac aactacacat
     1021 tttgacaaag aaacgctgac tgcaccaatc atcatggcac cagttgctgc tcatggctta
     1081 gctcatgtga aggcggagaa agcttctgca aaaggtgtag cagattttgg gacgatctat
     1141 acagcaagtt cttacgcttc ttgtacatta gaagagataa gagaagcagg cggggagaaa
     1201 gcaccgcaat ggtttcaatt ttatatgagc aaagatgatg ggatcaattt ggatattctt
     1261 gaagtggcca agcgtaatgg agcaaaagca atcgtattga cagcggatgc aacagttggc
     1321 ggaaaccgtg aaacagaccg cagaaatggg tttaccttcc cactgccaat gccgatcgtt
     1381 caggcttatc aatcaggagt aggccaaacg atggatgctg tgtacaaatc ttccaaacag
     1441 aagcttagtc caaaagatgt agaatttatt gctgctcatt ctgatttgcc agtctatgtg
     1501 aaaggtgtcc agtcagaaga agatgtctat cgttcattag aatctggtgc tggcggaatc
     1561 tgggtatcta atcatggtgg ccgtcaacta gatggtggtc cagcagcgtt tgattcgttg
     1621 caatatgtgg cagaagcagt agataaacgt gtaccaatcg tgtttgacag cggtgtacga
     1681 cgcggacagc atgttttcaa agcaatcgca tcaggtgcag atcttgttgc tattggcaga
     1741 cccgttattt atggattgtc tttaggagga agtacaggag tccatcaagt gtttgatttc
     1801 tttaaaacag aattggaaat ggtcatgcaa ttagctggaa cacaaacagt agaagatatc
     1861 aaaaaaataa aattgcgtga gaatcgtttt atttaagaaa caaatgacga aaaaaatagg
     1921 agtgcggcag atgatgccgt actcctattt ttatagttca aggatgtcac taatagaata
     1981 atttgaaacg aaaaaataaa attgaaatat tttttcatac t
//
LOCUS       DACDUU010000157         2003 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.160, whole genome shotgun sequence.
ACCESSION   DACDUU010000157 DACDUU010000000
VERSION     DACDUU010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.160"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..378
                     /locus_tag="IWL30_002876"
     CDS             <1..378
                     /locus_tag="IWL30_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAP9707567.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="IWL30_002877"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="IWL30_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP9707568.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2003
                     /locus_tag="IWL30_002878"
     CDS             1859..>2003
                     /locus_tag="IWL30_002878"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9707569.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DACDUU010000158         1959 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.161, whole genome shotgun sequence.
ACCESSION   DACDUU010000158 DACDUU010000000
VERSION     DACDUU010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.161"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..535
                     /locus_tag="IWL30_002879"
     CDS             <1..535
                     /locus_tag="IWL30_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9707570.1"
                     /translation="VVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDFPEESFDVIL
                     SSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNEKGEILHFPV
                     DNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPENMMDIPGMA
                     DEMRRPMMLIVSAKKKM"
     gene            568..1476
                     /locus_tag="IWL30_002880"
     CDS             568..1476
                     /locus_tag="IWL30_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAP9707571.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            1473..1721
                     /locus_tag="IWL30_002881"
     CDS             1473..1721
                     /locus_tag="IWL30_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin acetyltransferase"
                     /protein_id="HAP9707572.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLLTRGAS"
     gene            complement(1724..>1959)
                     /locus_tag="IWL30_002882"
     CDS             complement(1724..>1959)
                     /locus_tag="IWL30_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP9707573.1"
                     /translation="RKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVKG
                     LESRFESILLAYSVLLSRAQRRFGGTLRYSLGY"
BASE COUNT          677 a          289 c          420 g          573 t
ORIGIN      
        1 tgtagtaggt gttgatattt ctcataaaat gctcgaagta gcaaaaggaa aaacccattt
       61 tccacagatt gaatatgaat gctgtgccat agaagatgtg gatttcccag aggagagctt
      121 tgatgtaata ctaagttcgc ttgcgtttca ttatgtagca gactatgaga atttaataaa
      181 aaagatatat aggatgctga aggctggtgg caatttagtt tttacagttg aacatcctgt
      241 ttttactgct catggaacac aagactggta ttataacgaa aaaggagaaa tactgcattt
      301 cccggtggac aattattatt atgagggcaa acggacagct atgtttttgg aagaaaaggt
      361 tacaaaatat catagaacac tgaccacata tctaaataca ctgctttcaa atagttttat
      421 aataaatcag attgtggagc cacagccgcc agagaacatg atggatattc cggggatggc
      481 ggatgaaatg cgacgcccaa tgatgctgat tgtatcggca aaaaagaaga tgtaataata
      541 tagaaaaaat aaacgaggag tatgtaaatg agatcagaaa aagaaatgat ggatttagta
      601 ctttctttag cagaacagga tgaacgtatt cgaattgtga cccttgaggg gtcacgcgca
      661 aatattaata tacctaaaga tgaatttcag gattatgata ttacatattt tgtaagtgat
      721 atagaaccgt ttatatctaa tgatgactgg cttaatcaat ttgggaatat aataatgatg
      781 caaaagccgg aggatatgga attattccca cctgaagaaa agggattttc ctatcttatg
      841 ctatttgatg attacaataa aattgatctt accttattgc ccttggaaga gttagataat
      901 tacctaaagg gcgataaatt aataaaggtt ctaattgata aagattgtag aattaaaagg
      961 gacatagttc cgactgatat agattatcat gtaagaaagc caagcgcaag ggagtatgat
     1021 gattgctgca atgaattttg gaatgtaaca ccttatgtta ttaaaggatt gtgccgtaag
     1081 gaaattttat ttgctattga tcattttaat cagattgttc gccatgagct gctgagaatg
     1141 atatcatgga aggtcggcat cgaaacaggc tttaaattaa gtgtaggcaa gaactataag
     1201 tttattgaaa ggtatatatc cgaggatttg tgggagaaac ttttgtccac ctaccggatg
     1261 gattcctatg aaaacatatg ggaagcatta tttctatgcc atcaattgtt cagggcggta
     1321 tccggtgagg tggcggaaag gcttcattat gcctatccgg agtatgatag gaatataaca
     1381 aaatatacca gggacatgta taaaaaatac actggtaaaa ccggctgcct ggatagcaca
     1441 tatgccgctg atatagaaga gaggcgggaa cagtgattac agaaatgaaa gcagggcacc
     1501 tgaaagatat cgataaaccc agcgaaccat ttgaggtgat aggtaagatt ataccgaggt
     1561 atgaaaacga gaattggacc tttacagaat tactctatga agcgccatat ttaaaaagct
     1621 accaagacga agaggatgaa gaggatgagg aggcagattg ccttgaatat attgacaata
     1681 ctgataagat aatatatctt ttaacccgtg gtgctagtta attttaatat cctaaagaat
     1741 atctcaaagt tcctccaaaa cgtcgttgtg ctcgacttaa tagaacactg taagccagta
     1801 atatgctttc aaatctactc tctagccctt taacagaacg tgagttgaaa ttttgacaac
     1861 ctaatttttc taaggaagaa aaaacagtct caactgtttt tctttgtttt ccaagtaagc
     1921 tgtggtctag tttttctgat ttatccatgt ttttacgtg
//
LOCUS       DACDUU010000159         1939 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.162, whole genome shotgun sequence.
ACCESSION   DACDUU010000159 DACDUU010000000
VERSION     DACDUU010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.162"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            5..907
                     /locus_tag="IWL30_002883"
     CDS             5..907
                     /locus_tag="IWL30_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9707574.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            939..1181
                     /locus_tag="IWL30_002884"
     CDS             939..1181
                     /locus_tag="IWL30_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9707575.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(1437..1808)
                     /locus_tag="IWL30_002885"
     CDS             complement(1437..1808)
                     /locus_tag="IWL30_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707576.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT          751 a          320 c          380 g          488 t
ORIGIN      
        1 atccatgaat aacaataaca aaaataataa caataaaatg aacaaccaaa aaccagtgtc
       61 tgcacatcca gacccaacga caccaaacca atcaaatggt gtgaacgatg aaaagaacaa
      121 aacagcacca gtatctgcac atccagatcc agtagtccct aacgttcaac aaggagtaga
      181 agagaagaaa catgatgcag aaaagcatgc acctcatcat ccaggaactc cagaatctaa
      241 agttccagaa caacatgatg caatcgatcc ttctaaacct gtggaagagc aaaaacatcc
      301 ttctgaccat gcgaaagcac aagaaaaact tagttctgac cacacaaacg gacaaggaaa
      361 acttggttct gatcatacaa ataaccaagg aaagcatgat tctgaacagg aagattcaat
      421 gcgcggtgag ctgaattatg aagataaagt tgtgcaaaaa atcatcggaa tcgcaattga
      481 acaagtagac ggtttattat ctgcaaacgg tggtttcttc tctaatgtgg caggaaaact
      541 tgtgaataca gataatgtaa cggctgggat cgaaacagaa gtcggtaaaa aacaagtagc
      601 agttgatatg gatgtcattg tggaatatgg caaagatatc gaaaaaatct ttgaagaaat
      661 gcaagaagta atcggcaaag aagtaaaaaa tatgacacat ttagaagtga tcgaagtcaa
      721 cgctaacgta gtggacatca aaacaaaaga agaatttgaa aaagatcaag aaacggtgca
      781 agacaaagta actgacgcag cgaaaaaaac cggtgaattt gcttcgaatc aaacagataa
      841 agtaaaatca gcagctggaa gcggcgcaca aaaagtaaaa gaaaatacag aaccacgggt
      901 tcagtaataa ggaagtaaaa cattaggagg aacgaaacat gggatttatt tggtcactta
      961 tcgtcggagg aattatcgga gcaattgcag gagcaatcac gaataaaggt tcttcgatgg
     1021 ggattattgc aaatgtcgta gcaggtttag ttggatcagc acttggtcaa gcattattag
     1081 gttcttgggg accaagtatt gcaggaatgg caatcgtgcc atcattgatt ggtgccatca
     1141 ttttagtact aatcgtatca ttctttgctc gaaaactgta aaaagacttt taaaaagagg
     1201 tcgtgaaaaa gctgttcttc atcaagtaat aagaacagcc aaacaaaaat agcttctcat
     1261 atttttcatt tggttgggca tattatcgaa gatgcaagct tttgaacact gtttatacgg
     1321 aataagaggc tgtgacaaga cttttgtcac agcctcttat ttatgcgggc aaattcccaa
     1381 ttagattttt aaaagttttt ccttaaatgt gtctttattg atacagagaa gcttctttaa
     1441 taaaacaatg cctccctaaa ttttcttgag ataggaaagg tgaacataca aaataatacg
     1501 accactttga aaacccagat gatccacaaa tggatcgtat gatactgatg aagtaaaaag
     1561 caatggatca gaaactcaaa gagattgaaa aagacaagga agaaataata tctctttttt
     1621 actcttgtct gttttagcca ataatagctc gtaaagtaat ctagacaaac aaatagaagt
     1681 aaacaaagtt tcgtaagagc agcaatcgta tcatgttgct ctgctagacg attgacgagc
     1741 aggaaaaatg ttggtaatgg aatatagatc acgagtaatg aaagaaaaaa gtaaacaata
     1801 aatggcatac tattcctcct aattgtgtga gttcgccctc attttaaaat aacgatattt
     1861 gtttgtgaag ctttttcgtg tcgtaacaaa tgttatctaa tgtattttta aataaataat
     1921 cattattatc aaaataatg
//
LOCUS       DACDUU010000160         1932 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.163, whole genome shotgun sequence.
ACCESSION   DACDUU010000160 DACDUU010000000
VERSION     DACDUU010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.163"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..245)
                     /locus_tag="IWL30_002886"
     CDS             complement(<1..245)
                     /locus_tag="IWL30_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9707577.1"
                     /translation="MEVIIMNVGFIGAGKVGCSFGKYFQEHKIQVTGFYSKSEDSSLA
                     ASNFTSSKQYLNLRELVDENDTIFITTPDGQIQEVWQ"
     gene            483..1652
                     /locus_tag="IWL30_002887"
     CDS             483..1652
                     /locus_tag="IWL30_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9707578.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
BASE COUNT          686 a          323 c          300 g          623 t
ORIGIN      
        1 tcttgccata cctcctgaat ttgaccatca ggagtcgtaa tgaaaatagt atcattttca
       61 tctactaatt ctcttaagtt taaatattgt ttactagatg taaaattcga agctgctaat
      121 gacgagtctt cgcttttact ataaaatcct gttacttgta ttttatgttc ctggaaatac
      181 ttcccgaatg aacaacctac cttgccggct ccaataaaac caacgttcat aattatcacc
      241 tccataaaga agtgagacta caagtcccat tcttacgata tgagcttaaa aataaaaatt
      301 aaatcaaata tacagtatgt ttttttcaaa tatcatccat agttaattta tcataatatc
      361 ccatatttta aaataaaaga tagcgtaaaa tattttgtgt aaatagaaat ttagggtaga
      421 aaaaaaagtc cattctgtaa aattaaagtt accacacaga aattttatag gaggactttc
      481 tcatgactaa atttactata gaaattatgc aaacactact caataaaggg gatttagatg
      541 aattatttca ggatcattta gagcgagcga ttaattcact tctacaagca gagctaactg
      601 cttttttaga ttacgaaaaa tatgatcgaa atggattcaa ttcaggaaat tcccgtaacg
      661 ggaattattc acgtacattt aaaacagaat atggcgagtt gaatttaact attcctagag
      721 atcggaatgg cgaattttct caacaaacac taccagcata taagcgaacc aatgattcat
      781 tagaaacgac cattatccaa ttatttcaaa aaggcattac tatggctgaa atctccaaat
      841 taattgaaaa aatgtatggt caccactata cgccacaaac aatctccaac atgagtaaat
      901 tggtggctga agatgtttta gcttttaaag aaagaacgtt agaagccaat tattccgtta
      961 tatttatgga tgcgacgcat atccctgtga agcgacagac tgtttcgaag gaagcagtgt
     1021 atattacgat tggtattcgt ttggatggaa ccaaagaagt tcttgggttt actattgccc
     1081 caactgagtc tgcttatatt tggaaagaag ttcttcaaga tcttagaaaa cgtgggttag
     1141 aagaagtttt attagtagtg acagacggat taagctgtat tgaagaaagt atccatagtg
     1201 tgtatccgaa tgcccaattt caacaatgtt gtgtgcatgt atctagaaat atcgctcata
     1261 aagttcgtgt tcgaggtcga aaagaaattt gtgaggattt caaattggtt taccaagcga
     1321 attcaaaaga agaggcattg gatcacatcg actttatgac taggaaatgg aaaaagcagt
     1381 atccaagagt cgtcaattta ctcttgaatc ctgccctatt aaccttttat aatttccctc
     1441 acgccatcag acgaacaatt tattcgacga acctgattga aggctttaat aagcagctaa
     1501 aacgatatac tcgaagaaaa gaacaattcc ctaatgaaga atctctagag agattcttca
     1561 tttctcaatt taatcaatat aaccaaaaat ttttaggtag aattcacaag ggatttaaag
     1621 aaattcagga tacattagag tcgatgattt aactgtaatt aacagaatgg attttccatt
     1681 tacatataat tcttgacgct accataatct aaataaccat catattcatc tactacttat
     1741 ataattgagt tttattaaac tttttaagtt tctattctat caaatattta aacaatctct
     1801 gcatccgaaa agactacctc gtatagtttt actaacaata tttttattag cgtgttcaaa
     1861 tcctgatttc ttgtatcaat tattttctgt tcactttcaa taagaaaaaa gtctttactt
     1921 ccatggataa ta
//
LOCUS       DACDUU010000161         1904 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.164, whole genome shotgun sequence.
ACCESSION   DACDUU010000161 DACDUU010000000
VERSION     DACDUU010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.164"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            57..1901
                     /locus_tag="IWL30_002888"
     CDS             57..1901
                     /locus_tag="IWL30_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707579.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT          625 a          349 c          315 g          615 t
ORIGIN      
        1 cgttagttta aaaaacgttt accctaacat tacaagtttt agaaaggaat acctttatgt
       61 ctttttttaa taacatatat aataaaaaag aatcttttta tttacgtata aagtggagca
      121 taatcattat attatcattg ttatttttaa caggaattgc ttatttcttt tattttattt
      181 tttctcctgc ggacagcaat caaatgaaag aaataaaaga ttcgtgggtt ttctatacaa
      241 aagatgatcc tgattttaag tttgacagca agtacactcg tacgattcct accgtggata
      301 aagacgaaac attcattatg gaaacgacac ttgaaaaacc gttggacgaa gcaaatttac
      361 taatcaaagg aaaccatcaa tggattactg tatatctagg cgaaaagatt ttataccaac
      421 ggaaagatta tcaggaagaa agcaatcccg gactctcatt ggctgtgata gatttaccag
      481 caaattatat cggaaaaaaa ttgaccctta cagtctcatc tccttatcag aactatgctg
      541 gactgccacc gaaagtttat ctaggaacaa ctggaccatt gattagtttt atttattcac
      601 aatcagtgcc gcaagtattg acgatgataa tcgctgtttt tatcgctata gggacctttt
      661 tctatacgat ctacttgatg tataaacaaa aacgtcttga atactcactt attctcttaa
      721 gttgctttgc tttggcatta ggatttgaag ctgtatcaga agatatttta agtggtattc
      781 tttttgaacc attcgttcat tcttttttat ctcatttatt tattatctta acgtcagctt
      841 tcatcatctg ctactattgg agccgaatgt tttactataa aaaatggtac ggaatttttt
      901 gtacgttcca acttttgatt tttatcggcg ttctgcttta tgcaactttt actcctgcag
      961 aacttcctga attaatgccg attgctaata ttgctagtgt tataagcact ttagtcactt
     1021 cacttgcatg cattggcgaa gcatataaga ataaccgttt ttatgtagct tgtacacctt
     1081 ggatcgtctt ggtagccatt atccattgct tgatttacat ccaaacggca ttaggtatct
     1141 atcaaacaac gatcaattgg tctacgattt tatatattat cattctaatc gttattatca
     1201 gctacaatct aatcgatcac atcttaaata ccgacaaaga tcggagacaa gtagatttct
     1261 taaaaatcaa aaatgatttg ttggagcaac attatgagca gcttcgacag catatccaag
     1321 aagtaaatac acttcgcctg gaatttgtcc aagagatgga gaatctgcaa gatttaatgc
     1381 atacggacca aacgaaagct aaaaactatg tggaaaagat attagaagaa gcaaaaaatt
     1441 tcgaaatgct gtgttctttt tgcaaccacc aggtgacaaa tcttatctta gcgcgttacc
     1501 agaagttagc tgccaaacgg aatattcaga taaccttcca agcagatctt cccgaagaac
     1561 ttcctatcaa cgatgacgat ctggctcaac ttttgatcca cgctttagag cattccttta
     1621 gagaaactca tgccatcgaa aatccactgt atcgaaaaat ttatttgtcg atacacgaac
     1681 aagaggaaca tttcgtcatt tgttgcgaac atagtgcaca ttacgataca aacctattta
     1741 gtcgaggcat cacagaagaa ttagatgatc aggaacgttt tgacttaatg atgatgaaag
     1801 acgtagcgga tcgttacaca ggaacactaa cgcaggaaaa agatactttc attgatcgga
     1861 tcactgttca actctctcga aattacccga atcctgtata aaaa
//
LOCUS       DACDUU010000162         1891 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.165, whole genome shotgun sequence.
ACCESSION   DACDUU010000162 DACDUU010000000
VERSION     DACDUU010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.165"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..688
                     /locus_tag="IWL30_002889"
     CDS             <1..688
                     /locus_tag="IWL30_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707580.1"
                     /translation="KKSIFSTSKNVQRVFKILNKNSIFKKKIDDYFVYCPELLLYDVK
                     NIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYGIDETDIFAKVNS
                     IFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTLLISPISTISFSP
                     SMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLSEEKILLPENWAE
                     LRKLVEYGII"
     gene            675..>1891
                     /locus_tag="IWL30_002890"
     CDS             675..>1891
                     /locus_tag="IWL30_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP9707581.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYN"
BASE COUNT          637 a          239 c          295 g          720 t
ORIGIN      
        1 gaagaagagt atattttcaa caagtaaaaa cgttcaaaga gtttttaaaa tattaaataa
       61 aaatagcatc ttcaaaaaaa aaattgacga ttattttgtc tattgtccgg aattgttact
      121 ttatgatgta aaaaatataa aaaaaattcc caaaatagat gaaaacagta aattgatgaa
      181 aaacatacaa tcaatctttg ggatagaaga agtttttgtc ggagataaat gtatcttttt
      241 tgatcaacct ttattatctg atggttatgg catagatgaa acagatattt tcgctaaagt
      301 aaatagtata ttttctggaa aaaaagaaac tttaattaag cttcatccta gagcaaggac
      361 aaatcgttat ggtgaaaaaa acatattgaa ttccagttta ccgtttgaag tcttgctact
      421 tgaaaatgac ttctcttcaa cacttttgat tagtcctata tctacaattt ctttttcacc
      481 atctatgatg tttgaaaaga cgatcaactc tattatgttg gctaaattaa ttttaaatga
      541 atatacagca ttatctgaag ataaaaaaga aacattgaca aatattgttc ttttctgtga
      601 acattataac cgtctatcag aagaaaaaat tttactccca gaaaactggg cagaattaag
      661 aaagttggta gagtatggga ttatttaaca cactttccaa gtcaaaatta gttaagtcca
      721 gtttctggta tactataggg aattttttta ttaagggaat ttcattcata actattccta
      781 tttttgtaaa tattactact caagcagaat atggtttgat taataatttc acttcatttg
      841 tatcaatttt ttcgattctt gttgggctaa gcctaaattc ggcaattaat aatgcaagtt
      901 ttgactataa agatgacata aatgaattta tgtcatcggt actttctcta gctacacttt
      961 cgttaattgt gttcttatta atagggaaca tatatttttt gttttcaaat gctttttttg
     1021 atatgaacca acttgtattt aatttgatgt tattacaaag ttttgctagt tttgtaatta
     1081 gttatatcca gtcttatttt acgattaact ttaaccattt taaatatttg attttatctt
     1141 ttatatctac ttttttgaat attgcaatct ctgtagcttt gatgcagaca ttatattttg
     1201 atgataaata tgttggcaga gtagtcggcg gaacactgtc atttgtgttg ataggtttag
     1261 taatttattt aagaataatg ataaagggtg ctcatttttt tgataaaaaa cagtggagat
     1321 acgctttaaa aatctctttg ccgctaatcc cacattcctt agcaaatata gttttagcac
     1381 aattcgatag aattatgatt aattcttatt taggtcctgc tgcgggggga atatattcct
     1441 acattagtaa cataggtatt attctaagcg taatatggat gtctactaat aacgcctggg
     1501 ttccatggtt ctatggtgag atgagtaaaa ataatgaaaa aaaagtgaag aaagttagta
     1561 actattattt agtagtcttt actttgatta cgatgattgt aatggtagtt tcgattgatt
     1621 tagcaaaatt tatggctccg aaagactatt attctgggtt atctttagtg gttccgataa
     1681 ccttaggcta tttctttcag tttttatata gtttaccagt taatgctgaa ttttatgaga
     1741 aaaaaacatc gtttattgcg attggtacag tagcaagtgc tatagttaat atactattaa
     1801 acatgatttt catacctagg ttaggaataa ttgctgctgg atacacaacc gtatttacgt
     1861 attttttact ttttattttt cattataata t
//
LOCUS       DACDUU010000163         1847 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.166, whole genome shotgun sequence.
ACCESSION   DACDUU010000163 DACDUU010000000
VERSION     DACDUU010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.166"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            460..792
                     /locus_tag="IWL30_002891"
     CDS             460..792
                     /locus_tag="IWL30_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP9707582.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1411..1752
                     /locus_tag="IWL30_002892"
     CDS             1411..1752
                     /locus_tag="IWL30_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP9707583.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          642 a          249 c          404 g          552 t
ORIGIN      
        1 cttgtgatga attgattttc gttttgcact tttggatgat aaatttggac aattgaaaat
       61 acaaaaaaaa gaaaggcggt atgaactcgg aaggctggta accttcgtca gcgttcatac
      121 acggcattaa gatttggccc ttaatgcgtt aacctttcag tgttacggta ttacccgcaa
      181 tggatttagt ataacagata ttcaaaagtg ataccacttt ttttggctgt gttaaggtta
      241 aaaatacgtg atattacgat gaatctatgc ctaagcataa tgttataaaa atgtccgtgt
      301 ggctctttgc cgtatgacga acgctgaaat tagagggaac gcatgtttgg atgcgttccc
      361 tcttttttgt ttgtcatgaa cgaaaatcaa ttctctattt ttgaaaaata acaagaaaaa
      421 cttgttaaaa atatttaaag aaattgggtg gtttatttta tgtcatatac aggaattttg
      481 agtctcaaag atatttgtca ctacggtaaa cggtgtaccg ctacagagaa aatcacgaag
      541 aaactatcaa ctggccaaag taaaaccgtt gtgcagtgta agaaatatat aatccagaaa
      601 gacaaagtct cagaggaaat gatttattac attgggaaac aaaagcaaat aattttaaaa
      661 gatcctatcc ccttaaaaga gctatatcca acaatcaaac atgtttatga ccaaaacggt
      721 gtactgattg gtcgaagaaa aaatggagtc ttgcgttgta ccgcaaaagg gatggggcgt
      781 ttgatcggct gaattgatca ttctcataac ttgtatttta tcgtttttcg cttgcttgaa
      841 aatattgtcg agacttctaa aggaacttag agtcactaga aaaataagga gtagacttga
      901 tctatcaaca attcccttcg gggagtgacg gggtaggttt taacgattgg taacgtaaaa
      961 aattggtcaa tcgagtaaat gcgcttgttt ggatagtggt gaaatgaatt tgtgtaaata
     1021 ataagcgcag agtaaacaag agggaattta gaagttagac aagttagaat agcgtctaga
     1081 ttaatcatga gcagctacta agggatctag actttgacgc aaactcatgg aataaggagt
     1141 aggtcttctc gtaatgggga ggacgcacaa gaaattgtgt cgtgtgctgc agtacatgta
     1201 gatacttgcg aggacgttga ttattggatc aatcgagcat ttaccgctgt ctactagggt
     1261 ttctcgtttc ggacgccata aacgagtcga aattttttat gtatttttaa gtaaacgaaa
     1321 aacgataaaa tatgaacaag tcctgaataa agtgaaaaaa ttaatataaa gcatagagaa
     1381 aatgaaattt acgtaatgga ggaatgacaa atgaaaaatt atgatccgaa tatacgattg
     1441 ggcacacata ctattaaggt ttctttccaa cgttgggatt acaaaggatt tgtgacgttt
     1501 agaaggggcg gaaattgtaa aggattagat gttttagcat tagacgagga tgatttatac
     1561 gatcaaacat tgactgataa tccaattgga ttcggcttgc tacctgaaga tgatgaaggt
     1621 aatgaatggt ttaaaatgac gcttatgaat gataatggag acgaattatc tgttgaagat
     1681 acttggtcat atttgagtga ttatattgtc agctttgaaa tcattgaatt tgtagcggat
     1741 aaagaagaat aaaaactgga ggaataaatt ttacagataa aggcttagaa ggagtcacga
     1801 cgattaataa aaaggtagta ttaccttagg cattgagtaa ggaattt
//
LOCUS       DACDUU010000164         1799 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.167, whole genome shotgun sequence.
ACCESSION   DACDUU010000164 DACDUU010000000
VERSION     DACDUU010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.167"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            144..542
                     /locus_tag="IWL30_002893"
     CDS             144..542
                     /locus_tag="IWL30_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP9707584.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            526..1221
                     /locus_tag="IWL30_002894"
     CDS             526..1221
                     /locus_tag="IWL30_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAP9707585.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
BASE COUNT          684 a          266 c          343 g          506 t
ORIGIN      
        1 tccatacatc cattgtactc tgagaaatca cacaatatcg aaacaaattc cgttaggaat
       61 gtgttattca ataaatttta taatctagaa acattttgaa taaaaactat tagacaaatg
      121 atattttacg aggaggaaca gagatgacga tctttgaaac ggttgtaaca gatgttggta
      181 tagaagcaga agtattcagg aaagaaaaaa taatgatttt atttggagat gaagcaccgg
      241 aaacattaac agattattgt tataagattg aattgaatca ggtaagaaaa gaaataactt
      301 gtgatatgaa gctctgtttt gataaacaaa tgtatgtaat cacagcagta ggagatgttg
      361 tccaaactaa tttgaatgaa ttaggacata tcacgataaa atttgatgga tctcatgagc
      421 cggaacttcc aggaacaatg tatgtagaag ataaggaaat gccgcattta agaaagggga
      481 caatcattaa aatcgatcaa gaggatcaga atgggggaaa gctatatgga aatcatgctt
      541 gatacagcaa atacaaaaag tatttattct tgtcaacagt ttttgaattt aacaggcgta
      601 acaacaaatc ctacaattct gaaaaaagaa ggagcgtcaa acttcttctc gcatatgaaa
      661 gaaatcaaaa aaataatcgg acagattcct atccatattc agacaatagg aagaacggaa
      721 gaagaaatga tagaggatgc tcgagttatc atagaagaac tgggaaaaga tacgtttatc
      781 aaggtgccgg tgaatcaagc aggccttcaa gcgatcaaaa agttgaaaaa agaaggttat
      841 ttgattaccg gtacagctat ttacacagaa ttacaagggt atttagctat taataatgga
      901 gcagattata ttgcacctta ttataacagg atgcaaaatc aaggcattga tgctatgaaa
      961 gtgattgatt cgttgcggac tgagatcgaa cgtacgcaaa gaccaacaaa aatattagca
     1021 gctagtttca aaaattgtga acaagtcact acagccattc gatctggagc tcatgcgata
     1081 acgttaggta aagagctact agatcaagta ttagagatgc cctcgattga taaagcagtg
     1141 aggggcttcc aagaagattg ggtgcaaact ttccaagcag aaacgattgg tgaactaaaa
     1201 aaggtcgctg attacttctg aatcaatttc tccataaaat aaacgatagc cttctcgaaa
     1261 actcgaggag gctatctgac gattcaatat tcattaattt aaagtttggg tgactattac
     1321 taactattac ttatttctca tttaaacgat gtattcccta acctctaata catccttgta
     1381 actctgaagt tacgccagtt ttgaataaat ttcataaaaa caaatgaaaa gtgtcgcata
     1441 aaaatatctt tcgtgtttct ctagtataac agaataagaa gaacaagtcc agaagggata
     1501 tttttagtct tttttaaaat aacttttcag tcgagtttga gtttatgata gttgtttttg
     1561 aagcgatacg gcaagtgaaa aaatagggaa gcatgaaaga atctacttaa tggcgcattg
     1621 attcatgtga tagaaataaa aaactttttt aaattatatc ctcatgattg taacaaatcc
     1681 agtgatttgt tttggtacaa ataaaaaaag gccccgatga agttgtggca aaagtggttt
     1741 gcctcaaaaa taagaaggaa tttccaaaaa tcacttctca aattttgcca catccccaa
//
LOCUS       DACDUU010000165         1784 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.169, whole genome shotgun sequence.
ACCESSION   DACDUU010000165 DACDUU010000000
VERSION     DACDUU010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.169"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..605
                     /locus_tag="IWL30_002895"
     CDS             <1..605
                     /locus_tag="IWL30_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9707586.1"
                     /translation="VKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQSEDLLK
                     ALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEEWERHEL
                     LSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFFYPILVN
                     KEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            618..>1784
                     /locus_tag="IWL30_002896"
     CDS             618..>1784
                     /locus_tag="IWL30_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAP9707587.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVA"
BASE COUNT          660 a          208 c          359 g          557 t
ORIGIN      
        1 tagttaaaac attagaaaaa aatgatgtaa catcgattaa tcgattaaag cactgggtat
       61 ctctaacaaa agaattgtct aatccatctt tgaagaggga aatagaatat tctcagtcag
      121 aggatttatt aaaagcttta aaatggtcgg aagaagtcaa tcaacagatt agtcaagcaa
      181 aaggattaga taaacccttt ttaagtgcta gagatactat aaaagctctg aaaaaatatg
      241 ggaagataat tattgtaagc tcagctaata aggaagctgt tcaagaagaa tgggaaagac
      301 atgaattatt atcattagtt gatgaactgt gttgtcaaga taaaggaaaa aaagaagata
      361 ttattcgaag tgtaattgaa aatggttgtg atttggataa gatattaatg attggtgatt
      421 ctcctggtga tctagaagct gcaaataaaa ataaagtttt cttttatcct attttggtca
      481 ataaagaaaa agaatcatgg gaaaacttaa gaactgatgt tttatttaaa tgtttatcgg
      541 gagattatgt agatattgaa caaaaatata tagatacttt ttggaaaaat cttactaata
      601 agtagaggag aaagaaaatg actataaaag tagatttacg aaaaaaacca tataatttag
      661 atgaagatgc tatttcttgg attgaagaga cgattgattc tatgactttg gaagaaaaaa
      721 taggtcaatt gtttgttaat atgggatcta gtcgtgataa aaattacttg agtaatatgg
      781 tcaatcagta ccatattgga gctgtacgat ataatccagg aactgctgat gaggtatatg
      841 atcaaaacta tatcttacaa acagaaagta aaattcctct attaattgct gctaatacgg
      901 aagcaggtgg taacggagct tgtacagatg gtactgaagt tggtgtagaa gtaaaaattg
      961 gtgcaacaaa tgatgcgaaa tatgcttatg aattagggag agtttctggg attgaagcat
     1021 cggctattgg atgtaactgg agttttgctc ctattgtaga tattaataga aattggagaa
     1081 atcctatcat ttctagtcga tcttttggac aaaaccctga taaagtttta gaacttagtt
     1141 tagcttatat gaaaggtatt caggagagtg gaattttgcc tgcagctaaa catttccctg
     1201 gtgacggtat tgacgaacga gatcagcatc tatcttttag tattaatagt ttaggtacgg
     1261 aagaatggga taattctttt ggaaaagtat actcaggctt aatagaagct ggattacctt
     1321 caattatggc aggtcatatc catatgccag cttattcaaa acattttaat cctgatctga
     1381 aagatgaaga tttacttcct gcaactttat caaaagaatt aatcactgac ttacttagag
     1441 gaaaactcgg atttaatgga ttagtagtta ctgatgctag tcatatgtta ggaatgactg
     1501 gggcaatgaa acgatcagaa ttattgccca cttctattgc agctggatgt gatctatttc
     1561 tattttttaa tgatccagat gaagacttta attatatgat ggaaggctat cgtaaaggaa
     1621 ttatcactga tgatcggtta ttagatgcct taaaaagaat tttagggtta aaagctaaat
     1681 tgggattaca caaaaaagca aaagaacaaa ttttattacc taagcaagaa gctctggaaa
     1741 aaattggttt aattgaaaat aaatcaattt ttaaagaggt ggct
//
LOCUS       DACDUU010000166         1719 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.171, whole genome shotgun sequence.
ACCESSION   DACDUU010000166 DACDUU010000000
VERSION     DACDUU010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.171"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..328)
                     /locus_tag="IWL30_002897"
     CDS             complement(<1..328)
                     /locus_tag="IWL30_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707588.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVY"
     gene            complement(330..1388)
                     /locus_tag="IWL30_002898"
     CDS             complement(330..1388)
                     /locus_tag="IWL30_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAP9707589.1"
                     /translation="MVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPGLLARQGF
                     IEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQIFYITLSS
                     SRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFSFGTNYAK
                     DFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVI
                     RPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAF
                     ICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFNKRDVISI
                     VFWYYLGSISYLYLKKEN"
BASE COUNT          679 a          281 c          205 g          554 t
ORIGIN      
        1 tgtaaacatt ggagaaaagt atttttgtga aataattttc cccctgtata aaaatttcat
       61 cataaacgga taaatcatcc gagaaaaatt gattatggtt agttaacaag ttaattttac
      121 gtttaaagga agaaccgatt ttccggtgat tcacaaaaac aacatcatta aatagcccta
      181 attcttttat acgctcacta tatcttctag cgttttcaaa ttcagaataa ataaataaat
      241 ctccttttga ttctcggtaa aaattttgat aaacatttaa tatattaatc aaatgtaaag
      301 gagtccaagc aatgaaagct gtcttcatat taattctcct tttttaaata caagtaagaa
      361 atgcttccca aataatacca gaaaacaatt gaaatgacat cgcgcttatt gaaaagtaca
      421 tgagcctcca ccatttcatc tataaaaata gatagtataa aaattaaaac aataccaaga
      481 aacgcataat tcactttact tctaaaaaat gttttgagta aactaaagat atttaacaca
      541 tagaaagcat agatacatat aaaagcaatt ataccacttg atacaagtac ttgaagaaat
      601 ccattatgca tgtttccatg tgctcttttt agttcattca catctgtagt atcaagatct
      661 acttttttca aaaattttgc atcgctattc ccatagaaat cggtctctcc ataaccaaat
      721 aacaaagttg gtcttataac tttaagcgca gctttccaaa tcgtaaaacg accagctgaa
      781 atttcggagt tatcactaga atgctgtaat attacttctt tttctggaag tggagtttta
      841 tctgattcat ttgactctat aacttctact tttctatcaa tcgatacagg aattgttgct
      901 aaaaaatcct tagcataatt agtaccaaaa gaaaaatata ccaatagaac taataatatg
      961 cttaccatcc atttgatttt tacaggtagt acttctctaa tgctctttct aaattttgaa
     1021 ttggcaaaca acaaaagaaa taatactaaa aaagaacaaa ttactacctg ggcacctcta
     1081 gacgaagaaa gtgtaatata aaatatttgt acgataaaat tcatcatata aaaaacacga
     1141 atcgtttttg ttatttttga aattaataca ctaattaaac ttaaaataat agaagtatat
     1201 ccaaacatag ctccaacatt tggacttgta tagacaccaa ataaccgatg ttcaataaaa
     1261 ccttgtcttg caagtaatcc tggcgttcct gttgggacat gataagaaat caaaaaaata
     1321 aattgataga tagatattag cgcagctaaa aaaattatga taatgaaaac attattaaaa
     1381 tacaccattt ttttcttttt ttcctcttca ctttgtgaaa agtctgcagg aaaaattact
     1441 atcaaagtaa tcagcaaata tccaaaatta taaatagatg gaattaaaaa attttctctg
     1501 ttaatgatta tagttaagat aaaactaatc attgctccaa tcagtagcca agaaaaacgc
     1561 attttaaaaa aatttttctt tataaaaacc tcatacaaaa ctataattcc accccaagca
     1621 aaagctattt tcatcgggat attagttaca ttaaaagtac taggcataaa ataaagcaga
     1681 caaatgatta aatttattac tcgaaacgca tctcgactt
//
LOCUS       DACDUU010000167         1659 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.172, whole genome shotgun sequence.
ACCESSION   DACDUU010000167 DACDUU010000000
VERSION     DACDUU010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.172"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(136..426)
                     /locus_tag="IWL30_002899"
     CDS             complement(136..426)
                     /locus_tag="IWL30_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707590.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(544..804)
                     /locus_tag="IWL30_002900"
     CDS             complement(544..804)
                     /locus_tag="IWL30_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707591.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(941..1354)
                     /locus_tag="IWL30_002901"
     CDS             complement(941..1354)
                     /locus_tag="IWL30_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAP9707592.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
BASE COUNT          630 a          267 c          243 g          519 t
ORIGIN      
        1 tgttactatg aataacataa taagaaagta aagtggattg atttgagatc tttttatact
       61 tcaaaaaaga taacacccaa ctttcaaaaa tatgttttgg aagttgggac aaacgagtta
      121 atacatttta atacattatt ttttattcaa aaaaaatagc acaatggcaa agatcgtaag
      181 agagatcata agccccaatg aatgactaaa aagaaaagca gaatcacatg ccatatcatc
      241 ccatatttct tcaagaaaat aaataagcgg gaaaaaggga aggaatagta gaacatcgat
      301 ccagcaccat ttttttcttc ttttgaaata acataaaaca ataaacatga acgtgcaggg
      361 aagtaaaaac aaagctttac tcataactaa caaaatacca gctcctttta tttttttctc
      421 ccccacattc aaagagtaac aatggtatgt acttaataat agaaaaatgg atcaacttgt
      481 atgaataaca gctgaaaaga tctgcaccaa ataaaaaact tccagccaaa actgactgga
      541 agtttatttt tttctgaaat attctaacac aaatatgaaa aagatggccg aagctaataa
      601 gccgatttcg tagcaaaaca atgagatacc agagtcgtaa taataaaatc ctctaatctt
      661 atggataaaa tagatcatcg gacaagcaga taagagtaag tatttgttag gccttttgaa
      721 taagtaaaag aaagcactgg tcattatgat aaatggtatc caaaacaaaa gtttacttaa
      781 gacaatcaaa tgatcaactc ccatattttt taccaagaga gatcataaca tctttatcgt
      841 aaaaacaaaa tatttttttg ctcattttga aaatttaaac atactcgttc ttatccaaat
      901 atataaaaat cgcccctcat tcatcgagag gcgataagtt ctaatttaac tcaatttttt
      961 ttgtttcttt tggctgataa gctgttttag gtaataccaa tttcagcaca ccgtttttaa
     1021 aggttccagt gattttttct tcatcaacat taggaagata gaatgaacgt ttgtaagaga
     1081 ccgaatgtct ttctttccgt acatagttgc catcttcttt atcttctgta tgggactcat
     1141 gattggcgga gatcgtcaaa gtattatctg aatattcaac tgtcgtgtct tctttgtcac
     1201 agccaggcat atcagcagtt agtttgtatt ctttttcatt ttcgactaaa tctacttttg
     1261 gataacttga aacacctaaa aaatcattga atgctggact aaacacgtca ttgaaatcag
     1321 ggaacatgtc gcgaatgcta ggtacatttg ccatttttga tcatcctttc aataagtatt
     1381 ttttatttat ccctatttac gtaataactc tattttgaaa aaaatgcaag tataatggtt
     1441 agctttgtga atatttcatc taatacgata caaaggctat ctttctaatt tgaaaaaggg
     1501 atcattaaaa taataagaag aaaatatgtc aaaaggtaaa aaagtaaatt cttctatcca
     1561 aatatagaaa aattgaatct agaaaaccgc ccgacaataa atttcattaa ctcagttgac
     1621 cgaaaatgaa gtagatacca aaaatttatc aagagagca
//
LOCUS       DACDUU010000168         1631 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.173, whole genome shotgun sequence.
ACCESSION   DACDUU010000168 DACDUU010000000
VERSION     DACDUU010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.173"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            121..1587
                     /locus_tag="IWL30_002902"
     CDS             121..1587
                     /locus_tag="IWL30_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAP9707593.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          458 a          294 c          335 g          544 t
ORIGIN      
        1 ttaaaaatta cttgctttta tagaaaatta gtttatattt cttaaataag ttttttagtt
       61 aaatttttcg gtaaagatgt ataatatttt ctgaaaattt aaagaattgg ggtatagatt
      121 atggaaaaac agcaagacaa gggcttcttc gggcagccga agggattgtc aactttgttc
      181 ttcactgaga tgtgggaacg atttagttat tacggtatgc gtgcattact tatctattat
      241 atgtacgata cagttgccaa cgggggactt ggattaccac gagcaacagc attagctatc
      301 atgtcgatct acggatcttt gatctacatg tctagtatcg tcggcggatg ggtatctgac
      361 cgagtgttag gagcaaaacg cactgtattc ttcggtggag ttttcattat gcttggccac
      421 atcgtcttgg ctttaccatt tggtgtaagt acattgtttg tttcgatggc attgattatt
      481 atcggaaccg cctttttgaa accaaatgtt tctggcatgg tcggacatct ttattcaaag
      541 actgacttgc gaagagatgc aggattttcg attttttaca tgggtgtcaa cttaggggca
      601 ttattagcac cactaatcgt tggtacaatc ggtcaagaat ataactatca tttagggttc
      661 tctttagctg ctattgggat gttctttgga ttattacaat acatcatcca aggacgcaag
      721 acattagacg gagtgggtac aacaccgccg aatcctttga caactgaaga acgtaaagga
      781 ttcatccgta acttgatcat cgcacttgtt gttatcgctg cagtattcgg cggggcacaa
      841 ctaactggtc atctgacaat tgactttttt gtcaatacaa tcagtgtatt ggggattatc
      901 ttaccgttgt attactttat caaaatgctg acttcaaaag atgtaactgc tgaagaaaaa
      961 ccaagagtat gggcttatat cccattattc cttgcagcaa tcattttctg gtcattacaa
     1021 gaacaaggtt catctatctt agctttattt gctagtgaac gtacacaatc tacgctattt
     1081 ggtgtaacga tccaagcaag ctggtatcaa tcattgaatc caattttcat ctttattctg
     1141 acaccagtat tcgtaacgat gtggacgaaa cttggtgaac gtcaaccatc tacagtagtg
     1201 aaattctctc ttggactttt atttgcagga ttatcgtact tgctattgat gttgccgggt
     1261 atgctttatg gtacaagtgg aaaagtaagt ccattgtggt tgttgggaag tttcttcatc
     1321 gtggttattg ctgaattgtg tctttcacca gttggtttgt ctgtgacaac aaaattagca
     1381 ccacgagcat ttgaatcaca aacaatcgct atttggttct tagctgatgc ttcttcacaa
     1441 gcaatcaatg cacaaattgc acgcttctac acaccgggaa cagaatctgc ttacttcggt
     1501 attatcggtg cagtagcaat cgtcggtggt atcttgctat ttactgtgaa agaaccaatc
     1561 aaaaaactaa tgggctctat tcattaatca atgaacataa aaacaggaat tgcttatttg
     1621 cagcagttcc t
//
LOCUS       DACDUU010000169         1626 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.174, whole genome shotgun sequence.
ACCESSION   DACDUU010000169 DACDUU010000000
VERSION     DACDUU010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.174"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            1..1589
                     /locus_tag="IWL30_002903"
                     /pseudo
     CDS             1..1589
                     /locus_tag="IWL30_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT          559 a          334 c          301 g          432 t
ORIGIN      
        1 accaatctct cgaggaaacg agtgaaccga tccgagaata tcttaatgat gaagggcttt
       61 atgacacact ccatgagtat caattaaccg gaagtgcaag aacaggcttc agtgagaatg
      121 atgaacagct tggtggacct ttatatgata atggcgaagt tgtttttaca gcgaaccaaa
      181 aaggaaatgg ctattggaag atcgaaaata aaaatcatta caccggttct ggatggatag
      241 aagatcagga cgcacccatc caaccaatcc aagaattccc tgttgacatc acgagctacc
      301 aaaccgttat agaagacaaa gaaaacatta cattgaccct aaaaaaacaa gctttcttac
      361 cttatcctta tggaaatact accttgactg atttttcatt agctagtgga caaacgcttt
      421 actatttgcc tgaaagccag cgattcattc ctgtcccgaa tgaatcgaca cgttctgcaa
      481 ctttacaaac agaaaggtta gtttttacga gagaagaatt aaaaagcagt cagttaaaag
      541 ctgatgattt agaaaatcag cagacaaatc ttcaattgcc aaaaagtttg cctaaacgta
      601 ttgaggactt ggcggtcgaa ttgaccaaaa atgaagcaac tttaattcgt aaagtcgaag
      661 ctgtctaaca gtatttaaaa acaagcggaa atttccgcta ttccaaaacg gataccgggc
      721 atactccgaa agaacacgat tatgtggatt atttcctctt tgattcaaaa gtcggctatt
      781 gtgacaactt ttcaacttct atggtcgtcc ttcttcgaac acttggtatc ccagctcgct
      841 ggacaaaagg ttttacaaat gggctcagaa caggcacaga taatcaaggg aatgaggaat
      901 acagcattct caatagtcat gctcattctt ggccagaggt atactttcct ggatttggct
      961 ggcttccatt tgaaccaacc ccttcatttg ccaatccaat gggttcacaa ccagttgctg
     1021 aaacaaccga aaccgtccag cctgctatcg aacaaccaaa tgaaagcgaa aatatggcag
     1081 aaactatttc cagtgagaga tctgaggtca ttccatcaga agaaacaaca ctcacaccag
     1141 cttataaaat cgtcttattc agccttggag tgtgtttatc cgtcttgttg ttctttctat
     1201 tgttattcag aaaatctctg cgctggacaa gttatcttat ctatcttcat ttttttgctt
     1261 ctactaatac gattgcatat caaaaacttc ttaacccttt ggaaacattg gtaccgcgca
     1321 aacccgcaga atctttgcag gaatatagcc tgcgaataga acaagaattc aagctagatc
     1381 tgcgtttcat tcgattaact actcaatatg aagcagaaat atatggacga caagcagatc
     1441 cggtaaaatt cgataaaaaa acctatttag aaataacgca tataataaga aaaacggtat
     1501 acaaaaaaat tctgacaaaa gggagccgct tcaaaaaaca agataaaatt tctggagaat
     1561 ctacttctcg aatgccgaga accagctgat tgagtatcga ttactcgaaa aaaaggttgt
     1621 gacacg
//
LOCUS       DACDUU010000170         1619 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.175, whole genome shotgun sequence.
ACCESSION   DACDUU010000170 DACDUU010000000
VERSION     DACDUU010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.175"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(370..1539)
                     /locus_tag="IWL30_002904"
     CDS             complement(370..1539)
                     /locus_tag="IWL30_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9707594.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          538 a          254 c          234 g          593 t
ORIGIN      
        1 gggatttagt ttatgatttt atatcatttc agtaactcag aatattccga cttttttgta
       61 caaaaaaatt aaccttactt atatgaagat tctaagctct tatttaaatt ttagacgggc
      121 agttatccgt agaagttctt agaatagcta atttacataa ttaaattata tgaaatttaa
      181 gtttgaacaa tatactaaga actcccctta ctataaagtg aaataaatta gcatatgaat
      241 aaccgtgcta ccaagctttt cattaataaa atatttttat taatatttta taactttacg
      301 caagatcata attttgtcgg gagcgtcaat aattttgtgt aaatggaaaa gcccattctg
      361 ttgatcaagt taaaacattg attctaatgt atcatgtatt tctttaaaac ctttatgaac
      421 tcggcataga aacttttgat tatagttgtt gaattgagat actagaaatc tctccagaga
      481 ttcttcatta ggaaattgct cttttctttt ggtatatttc tttaattgtt tgttaaaacc
      541 ttcaatcaaa ttagttgaat aaatagttct tctgattgac ggagggaaat tataaaaggt
      601 taatatagca ggattcatga gcaatttaac cactcgtgga tactgctttt tccatttatc
      661 tatcataaac cttatttgat tatttgcttc ttcttttgaa cttgcttgat aaactaattt
      721 gaaatcgcta cagatttctt gtcgatcact aacacgaacc ttatgagcga tatttctaga
      781 tatatggaca caacattgtt gaaattgagc atttggataa acactatgaa tactatcgtt
      841 aatgccactt aaaccatcag ttacgactaa taaaacctct tttaaaccac ggtcttttag
      901 gtcttgaaga atctctttcc aaacataagc agattcagtt ggagcaagac taaacccaag
      961 aacctctttg gttccatcta atcgaatgcc aattacaata taaactgctt cttttgaaac
     1021 agtttgtctt tttaaaggaa tgtgagtagc atccataaaa atgacagagt atctggattc
     1081 taaggacctt tctttaaagg caataatatc ttcagaaact attttagtca tatttgaaat
     1141 ggtttgtggc gtatagtgat gcccgtacat tttttcaatc aattcagaga tttcagacat
     1201 agtaattcct ttttgaaata gctgaataat agtggtttct aaagagtcat tggttctttt
     1261 ataagcaggt aaagtttgtt gagaaaactt accatttcta tctctaggaa ttaccaaatt
     1321 taattctcca tactctgttc tgaatgaacg tgaataattc ccattacggg aattacctga
     1381 attgaaccca gcacgatcat atttttcata atcaagaaaa gctgttaatt cagcctgcaa
     1441 taatgaatta acagctaatt ctaaatgaca tcgaaataat tcatctaaat cacctttatt
     1501 gattagtgtt tccataattt ctgtagtaaa attgggcatg agaaagtcct cctataaaat
     1561 ttcggttgtg gtaactttaa ttttacagaa tggactttct ttttttctac cctaaatat
//
LOCUS       DACDUU010000171         1600 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.176, whole genome shotgun sequence.
ACCESSION   DACDUU010000171 DACDUU010000000
VERSION     DACDUU010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.176"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            38..460
                     /locus_tag="IWL30_002905"
     CDS             38..460
                     /locus_tag="IWL30_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707595.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            470..673
                     /locus_tag="IWL30_002906"
     CDS             470..673
                     /locus_tag="IWL30_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707596.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            884..1468
                     /locus_tag="IWL30_002907"
     CDS             884..1468
                     /locus_tag="IWL30_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9707597.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          563 a          227 c          295 g          515 t
ORIGIN      
        1 aaacgttttc ttatttttga tttcgaggtg atagacaatg aaaaataagt ttattttatc
       61 tttaggtatt ttaactattg gaattagtgt aactggaact acaattccta catttgctga
      121 tagtacaaat gaaccaacta ctgtagtaga taataaccaa gaattaactc ctttagatag
      181 gaacggaata gccattggta acccaactaa cgacttaact gttactgaaa attatttaat
      241 agtttctcca ttacttgctc gagcagcttc taaaacgata cgagtgacag gaacgatgat
      301 ttttgataaa gttggttggc cttctagacc tcctcaatca cggaattgga aagaaaaaag
      361 aaatggttat tggtactatg gaaaattgta tttacaaagt tataaatcag ctggaaacaa
      421 ttggattgcg acttattcag gaaccttaac tagaaagtag ggatttttta tgccagaatt
      481 tgtgaacaca ccaaattttt atcaagatag cgatattcta agtaaaatta atttaccgaa
      541 agaagaatca gctttacaag ttcagcgaac tttacaggag acacaaaaaa ttcaaaaatc
      601 aatttctaat ctagaagatt ctgaagaaaa aagaaaaaaa agagaccaaa aaacatatca
      661 aaaagaaaaa taatgatttt aaaaaaagag tgtctagtcg acattctttt tttctaattg
      721 aaagtgttca taaaatcacc catttctgga cactttcttt ttttgcttta tttttcaaaa
      781 aaacaattca tttcatgttc ataaacccat aacaatatct atgttcagtt aaactatatg
      841 gataaagtta tgttacaata aaattaatga aggagtgatt tttttgaaaa taggatacgc
      901 ccgggtatct actggtttac aaaatttgga tttacaagaa gacagcctaa ataaatttgg
      961 ttgtgaaaaa atttttactg atcatatgag tggggcaaaa agtaatcgac caggattaga
     1021 aatggccatt gattttgttc gctctggcga tacacttgtc gtttggcgct tagatcgttt
     1081 aggtagaaat atggaagatt tgatttcaat cgtcaatcga ctgaatgagc gtggtgtgag
     1141 ttttcatagt attcaagaaa atattacgat ggacaaatca agttctacag gacagctcat
     1201 gtttcatttg tttgcggcat ttgcagaatt tgagcgtaat ttgatcctgg aacgatccgc
     1261 agcaggacga gcagctgctc gtgcaagagg acggtttggt ggtcgtcctg aaaaattgtc
     1321 tgaacaagat ttggaattac tgaagacttt agtggatagc ggtacaccta ttaagactat
     1381 tgcggaacgg tggaacgtct caagaaccac catttatcgt tatttggcaa aaatggagga
     1441 gaaaaatgga aagacaaatt ctcattagtt tttgtcattt aatgctgaaa atttttgcca
     1501 tttatctaga aaagtgacaa tagtatgaga attttttgta agtgtctact gataacttat
     1561 tctcaaatgt tgtaaggatt ttattcattt gttaatatta
//
LOCUS       DACDUU010000172         1568 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.177, whole genome shotgun sequence.
ACCESSION   DACDUU010000172 DACDUU010000000
VERSION     DACDUU010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.177"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(606..962)
                     /locus_tag="IWL30_002908"
     CDS             complement(606..962)
                     /locus_tag="IWL30_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAP9707598.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(976..1386)
                     /locus_tag="IWL30_002909"
     CDS             complement(976..1386)
                     /locus_tag="IWL30_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP9707599.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
BASE COUNT          485 a          280 c          222 g          581 t
ORIGIN      
        1 tgtttgttgt cttgaattga atcaagacgc cctacacatt tggtttgtct tactttgttc
       61 agttttcaaa ggtctacttc atcaggagtg ttccccgatt cgtttttggc tgccgtagca
      121 acttttatat catatcagag agacaagtaa ttgtcaactc ttttttaaaa gtttttttga
      181 tttcgttttt cgaaatctta cagcaactca tttatcataa cttatcattt gttatttgtc
      241 aagaactttt ttgaagtttt tttcgataga atcaagcttc acaagtggtt cttttttaac
      301 aactcagtta tcttatcatg tcatttctaa catgtcaaca actattttat atgtttttta
      361 aaatactatt ttattgcttt gctacttaaa acaacagaaa tgaatatatc atgtaaattt
      421 acacatgtca atatattttt aactctatat ccccacctag atttgaattt tgaccacata
      481 aattgagcat gtctctatag tagaaaaata aagtcaaaaa aagaacacat aacacgcttg
      541 atgctaagtg ttcgactgtt acaactgtat gaaatgctca gtgacttgat taaaacagtt
      601 aaagtctatt tatttaagat aagctgttcc atcaactgct cattgtatcc aataaataga
      661 acttcatcga taataatcaa aggacgtctg aggagattgg tatgttgtgt gcataaaaaa
      721 atcagttgtt tttttcctac acaggaaagg tcaatcgcaa tgttgtgaaa agcctcagac
      781 caagtcgcaa atagctgttc tgcttgcact tcttcaaaac tgcataattc ttctgcttct
      841 tctgtactta gatcgcgttc ttctaagtca acaaattgat aaggcaattg atggtgttct
      901 aaccattgtt tcgccttcag acttgttaca cagtgcgctg atccatagac tttcacttcc
      961 acgaaggctc cctccctatg aatttaagat gttattgttt tgtaaataga tcagatttct
     1021 ggcaacacat cgaatttcac gagggacaaa tgaccggatc tcatcttcat taaatccgat
     1081 tagtattctt ttttcatcaa tcaaaatagg tcgtctcaat aatgaaggat attctttgaa
     1141 taagacgatc atttgcttta aagttaattc gttaaaatct atgcccagct tttcgtaaat
     1201 tttcgaccga gtggaaataa tatcttgaac tcctccttca gtcgctgcca agatcttttt
     1261 gatctcactt tctgttaacg gttcggcaaa aatattcttc tcttcaaatt caagttgatt
     1321 cgcttgcaac catgctcttg cttttctaca ggaagtacag ctaggcgaag tatatacttt
     1381 tatcattttc tcactccttt ttaattataa tccaattcta cagtaatttc ttttaaaata
     1441 ccaattatta gctaataaaa tataaaaaaa atcaaaataa ctgattattt acgtttgaat
     1501 aatgagaatt tttctatata ttctctattt tgataatagt attctcattt tcaaaataaa
     1561 taagggag
//
LOCUS       DACDUU010000173         1536 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.179, whole genome shotgun sequence.
ACCESSION   DACDUU010000173 DACDUU010000000
VERSION     DACDUU010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.179"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..>1536)
                     /locus_tag="IWL30_002910"
     rRNA            complement(<1..>1536)
                     /locus_tag="IWL30_002910"
                     /product="23S ribosomal RNA"
BASE COUNT          321 a          476 c          337 g          402 t
ORIGIN      
        1 gggaaatctc atcttgaggt gggcttcaca cttagatgct ttcagcgttt atcccttccc
       61 tacatagcta cccagcaatg cccttggcag aacaactggt acaccagcgg taagtccatc
      121 ccggtcctct cgtactaagg acagctcctc tcaaatttcc aacgcccgcg acggataggg
      181 accgaactgt ctcacgacgt tctgaaccca gctcgcgtgc cgctttaatg ggcgaacagc
      241 ccaacccttg ggaccgacta cagccccagg atgcgacgag ccgacatcga ggtgccaaac
      301 ctccccgtcg atgtggactc ttgggggaga taagcctgtt atccccaggg tagcttttat
      361 ccgttgagcg atggcccttc catgcggaac caccggatca ctaagcccga ctttcgtccc
      421 tgctcgactt gttggtctcg cagtcaagct cccttctgcc tttacactct tcgaatgatt
      481 tccaaccatt ctgagggaac ctttgggcgc ctccgttacc ttttaggagg cgaccgcccc
      541 agtcaaactg cccatctgac actgtctccc accacgatta gtggtgcggg ttagagtggc
      601 cataacgcag gggtagtatc ccaccagcgc ctccatcgaa actagcgttc cgatttctac
      661 ggctcctacc tatcctgtac atgcggtaca gacactcaat atcaaactac agtaaagctc
      721 catggggtct ttccgtcctg tcgcgggtaa cctgcatctt cacaggtact aaaatttcac
      781 cgagtctctc gttgagacag tgcccaaatc gttacgcctt tcgtgcgggt cggaacttac
      841 ccgacaagga atttcgctac cttaggaccg ttatagttac ggccgccgtt tactggggct
      901 tcaattcgta ccttcgcttg cgctaagcac tcctcttaac cttccagcac cgggcaggcg
      961 tcagccccta tacttcatct tacgattttg cagagacctg tgtttttgat aaacagtcgc
     1021 ttgggcctat tcactgcggc tgatcgtatg atcagcaccc cttctcccga agttacgggg
     1081 tcattttgcc gagttcctta acgagagttc tctcgctcac cttaggattc tctcctcgac
     1141 tacctgtgtc ggtttgcggt acgggccgct gttttctcac tagaagcttt tctcggcagt
     1201 gtgacatcag gaacttcggt actattattt ccctccccat cacagcttgt ccttaagaaa
     1261 agaagcattt gactcctctt cagacttact gcttggacag acatttccga tcgtctgcat
     1321 tccttagcct cctgcgtccc tccattgctc aaacaaaaac aacgggtaca ggaatatcaa
     1381 cctgttatcc atcgcctacg cctgtcggcc tcggcttagg tcccgactaa ccctgggcgg
     1441 acgagccttc cccaggaaac cttagtcatt cggtggacag gattctcacc tgtctttcgc
     1501 tactcatacc ggcattctca cttctaagcg ctccag
//
LOCUS       DACDUU010000174         1442 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.180, whole genome shotgun sequence.
ACCESSION   DACDUU010000174 DACDUU010000000
VERSION     DACDUU010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.180"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(322..1362)
                     /locus_tag="IWL30_002911"
     CDS             complement(322..1362)
                     /locus_tag="IWL30_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP9707600.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT          427 a          226 c          200 g          589 t
ORIGIN      
        1 tattcgtatt gcctgaaaag aagtaatatc aagattttta gcttgtttaa ataatgaaat
       61 tatctctctt cgcgtattat tataactcaa aaaaaaggag tatgcaaaca tacgttccct
      121 tttctatatt tgaaattaat tatttctttt aacacttatg acgtaaaagg ctacgtttgt
      181 gatttttatc ataatatttt aacttataaa ttctattaaa atagctatta ttttgtaata
      241 ttcgtaatat aattagaata ataaaaaatt tttaatttta atgttactcc atgaagcaac
      301 aatcactttg tcagtaaagt ttcactctaa ccagtctttc gtaatagtgc ttggggagct
      361 tgcataattc aaacgttttt cacttgatac tttagcagct attggttttt cgataaacca
      421 attttttaca tagacaaaaa tcaggttctg gatattttcg actttttcgg tttttaattt
      481 ttgataggct tttaacagtg tcgtgtataa ttcttgttct gcattgacat aataactatc
      541 gagttcttca aaaactatcg aaatatcact ttctatttca gcttttttct ttgcattatg
      601 aatagtctgg acggtttttt gagcttcact gtaacttgaa gaaaatgttt ttatcaaatt
      661 taatactcgt tcagggacaa acgtagcaat gctgtcgttt gtcataattg tctcaaaatt
      721 gtctaacaac aattgatctt gtaggcggtc tttttcagtg tctatattgt gtctattagt
      781 gtctaatata ttattattaa gttctggtat gattttcata ccctctgatt tatcaacgtt
      841 ttggcacggt atgattttca taccctcttg agccccaagg gattcagcgt ctgatttttc
      901 gtcgagggtg ggtatgattt tcataccctc tgatttatca acgttttccc tattctctct
      961 atcaaatttt tcgagtaagt agatatcatt ttctgagact tttaattctg ctagatatag
     1021 tctattagga ttatttttcc ctaactgttt gttaaatccc atctgctttt gtaataataa
     1081 accaaaggat tccaactctt ttttgattgc tatcgctttt tgtttttggc agtttaatac
     1141 tctacatagt tcatttgtcg tataagtaaa ataaacatta ccttcttcat cgatttgccg
     1201 tttgctgatt gcgatctcta atcttgcttt taaaagcata taggcaatct tagttgaatc
     1261 tttcattttt ttgtattcgt cagaaacaat aaagactttt ggaaattgat aaaataattc
     1321 gttgtaaact ctatccgcct taaaaaattt aaaatcagtc attgtagatc ctctactttc
     1381 acgttgtttt atttatgtaa aattacctac tcttttcgaa aatagcaaag ataaataaaa
     1441 aa
//
LOCUS       DACDUU010000175         1426 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.181, whole genome shotgun sequence.
ACCESSION   DACDUU010000175 DACDUU010000000
VERSION     DACDUU010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.181"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(278..1030)
                     /locus_tag="IWL30_002912"
     CDS             complement(278..1030)
                     /locus_tag="IWL30_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9707601.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(1045..>1426)
                     /locus_tag="IWL30_002913"
     CDS             complement(1045..>1426)
                     /locus_tag="IWL30_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9707602.1"
                     /translation="ANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIES
                     QDRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINK
                     KNPNMFVNYQNDPYQFTTQVILLF"
BASE COUNT          460 a          252 c          174 g          540 t
ORIGIN      
        1 attagtagaa tttgccaaat ctttcgcatc ttccaccata catttaattt taattcccct
       61 ttatggtact taataaaaac aagaaaagaa ctattcattt ggcccttttt tggttccaga
      121 ccatagaaat attcctccag taactaatat tgctgttatg cacgtaacta ataacagcta
      181 taaaaattgg tacaacttta ataggattca gaaaaatttg cactagtgaa ggaatctatt
      241 ttattagaaa aatgcaatct ccctaaagaa aaaaccacta aacagtgctt tctttcaact
      301 tctccttaat tttttgtgct ttccctaaag taaagtttct ggccaactct gaatcaaaat
      361 atagtgaacg ttttttaaaa tctatttctt taacgttaaa gatatttact aaacacgctc
      421 gactaattct tgttaacata ggatatttct tttcaagctc actaattctt ccataaaatt
      481 cgtactgccc atctttagta cacaatgata atctatgtgg taattttgaa gcttctacaa
      541 aataaatgtc atttatatca aaagtaaatg tttgagaacc tatggaaaaa acaaatgctt
      601 gattcgtaac ctgttttgtt gcatctatcc tttcttgcgc taataacaat aactcaacaa
      661 tctcgtttcg atactcatct agtgtttgat cttttgttac aaatcctaac gtttcaactc
      721 ttctttttat tgttacgggt aacatctcat catgagtagt tgtaaaaatg atttttgctt
      781 gaacatcata ttttctgatt acttctgcta attctatacc gttaacttca tgatttaaat
      841 caatatctaa gaaatatatt ccttgctttg gtcggaattg tttgaggtat tttttaactt
      901 ctaacggact ttgtgttttc aatactatct taaatacttt atcgtgaaaa agaatgaaat
      961 tatcaataat tcgttctaac tgatttaatt gtataatttg atcttcacat aatattattg
     1021 gataactcat aaatcctcct tccctcaaaa caacaaaata acttgagtag taaattgata
     1081 cggatcgttt tgatagttca caaacatatt gggattcttt ttattgattt cctctatact
     1141 agataaacct aacccttgat gtcccttttt tgttgttatt ccttttttca ttagagtatc
     1201 tatagagagt agtatctctc tacaactatt cgatatcgaa atttctatct gtttatcatc
     1261 tcggtaaata gctagagaca gttctctatc ttgactctct atagatgctt caattgcatt
     1321 atcgagaata attccaagca ccctaataca gtcaaatatt ggaacaggaa cttcaataat
     1381 agtacttgga cattcgaatt tacaacaaat cttattgtta tttgct
//
LOCUS       DACDUU010000176         1424 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.182, whole genome shotgun sequence.
ACCESSION   DACDUU010000176 DACDUU010000000
VERSION     DACDUU010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.182"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(150..1337)
                     /locus_tag="IWL30_002914"
     CDS             complement(150..1337)
                     /locus_tag="IWL30_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAP9707603.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DACDUU010000177         1354 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.183, whole genome shotgun sequence.
ACCESSION   DACDUU010000177 DACDUU010000000
VERSION     DACDUU010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.183"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            76..>1354
                     /locus_tag="IWL30_002915"
     CDS             76..>1354
                     /locus_tag="IWL30_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAP9707604.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQ"
BASE COUNT          461 a          262 c          257 g          374 t
ORIGIN      
        1 ggtattaaaa aacatatcgt tctctagtat gattaaatca ccacaaaata aacaactgag
       61 aggacgatac gctatatgaa tgattctatc aaaaaaatgc tgagaataac agagaaagat
      121 ttgatgatta cagaggtctc ttacgagacc cttcagaaga aaaagacgtt ggtcgtcgat
      181 gctgttctct cgcctactcc tcgtgcttgt agaagttgtg gttctactgt ggtagatgga
      241 aacgggaaag caattatagt gaaaaatgga aaaaaggaaa cgattgtccg ttttgaacaa
      301 tacaatcata tgcctttggt tatgcgccta aaaaagcaac gctatacctg taaaaactgt
      361 cgcacccatt ggacggctca aagttatttt gtccaaccca gacattcaat cgcaaatcat
      421 gttagatata aaattgcttc tttactgact gaaaaagtat ctttatcttt tattgcgaaa
      481 agctgtcagg tatctttgac caccgttatt cgtacattga aagagtttaa aagctattta
      541 ccaaagcaat ctaagaagat cctccccaga gtattgatgg ttgacgaatt tcgttcgcat
      601 gcttccatag aagataagat gagctttatt tgcgcggatg gcgaaacagg aaaattaata
      661 gatgttttgc ctacgcgtaa attacctcga ttaacaagct atttcttagg ttgtaccaat
      721 ccagaagaag tagaattctt ggtgacagac atgaacgccg cctacttcca gctcaccaaa
      781 cgtgttctgc caaatgcgaa agtcgtgatt gatcggtttc atattgtcaa acacatgaat
      841 caagcgttca atgagttgcg tatccgtgaa atgaatgaac ttcgtaaagc cggacagaaa
      901 agccaggcag aaaaactgaa aaagaactgg cgctttttgc taaaaaatcg tgcaaacatc
      961 aaccattatg aatacaaaac atggaaaagt ttccgagcac caaaataccc atttcttact
     1021 gaagcaatga tgattgatcg attgcttgag ttttctccgc ccctaaagga ggcgtatccc
     1081 ttttttcatg aattagttga agcctttcga gacaaagacc ctgacttatt tttctcctta
     1141 ttggcagaac ttcccgaaac gttagatgac agctttcggg aaaagcttca aaaccttctg
     1201 acctatgaag aaggcatcac caacgcaatg atctatcctt attccaatgg aaaaatagaa
     1261 gcgaagaata cccacataaa gacaatgaaa cgagtatcct acggatttaa atcatttgag
     1321 aacatgagaa ttagaatctt tttgatcaat caat
//
LOCUS       DACDUU010000178         1329 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.185, whole genome shotgun sequence.
ACCESSION   DACDUU010000178 DACDUU010000000
VERSION     DACDUU010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.185"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            97..570
                     /locus_tag="IWL30_002916"
     CDS             97..570
                     /locus_tag="IWL30_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAP9707605.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            579..806
                     /locus_tag="IWL30_002917"
     CDS             579..806
                     /locus_tag="IWL30_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9707606.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          424 a          203 c          217 g          485 t
ORIGIN      
        1 aaacgatata tttttattgt ttttcgataa aaaatagttg ttttacaata attattctgc
       61 tatattttag ctatcaaaga taaaagaggg ataaaaatga aatttaaaac attgctatta
      121 aaatttgtcg ttttaggtat aagtgccgta tttattttgt tcggcgtact tttttatact
      181 cagatcgcta caagcgaaaa aggattcaat tttgattggc agaccacatt atttgtttct
      241 gtcatttact ttgcccttct cctagggatc gttattgctt atttcttatt gaaactattg
      301 aaaaaaatcg aagataacca agctttttcc actcaaagct taaacatact aaaaaaaatc
      361 gaacgaacca ttttgtttat tggtttcgcc ttttttggct tacttcctaa atttatgaaa
      421 gtagcggatt tagacgatgc tccaggtttg atgttggtag gaatcgctgt cgtattcatt
      481 ccttttgcag tatacacatt aacaagcgtg ctggaaaaac tgcttgaaca tgcgatttta
      541 cttaagacag atcatgattt gacagtgtga ggtgcgacat ggcaattatc gttaatttag
      601 atgtagtatt agctcaaaaa aaaatgagtg tgactgaatt gagtgagaaa gtgggcatta
      661 cgatggcaaa catctctatt ctaaaaaatg gaaaagcaaa agccatccgt ttttccactc
      721 tcgaaaaaat ctgccaagtt ttggagtgtc aaccaggtga tttgttgacc tataaaaatg
      781 actcgaatac tataccagaa gaataaaata aagtagaaca aacagtaaat aaaaaataat
      841 ctgaatgata gatgtgtgag acttttagaa gttatttacg tctattattc agatttttta
      901 tttcgattga ataccgtttt atctataaat aagatatgta ctgactcata caaagggggc
      961 cttttcttct ccatgtacta tcacttttcc tgatacaact ggtttcctgt tacgttgtag
     1021 ttggatctgc ttttagtata atactttatt cattccacca gactgatagc agtttgagca
     1081 gtctaaataa tcatgtagta ttacaggcca ttctgtttca taccttgaag tgcttaaaac
     1141 ttcagctaat ggcccattgt gttaactctt actccattct tttttagtaa gctcccatat
     1201 atactgtaaa gccttctata tattatgtca tgttgctttt taacatttac tatgtagttt
     1261 tgataaaatg atttgaataa cagtgatgga caattctttt ttatttttaa cattattttg
     1321 ataataatg
//
LOCUS       DACDUU010000179         1316 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.186, whole genome shotgun sequence.
ACCESSION   DACDUU010000179 DACDUU010000000
VERSION     DACDUU010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.186"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            66..>1316
                     /locus_tag="IWL30_002918"
     CDS             66..>1316
                     /locus_tag="IWL30_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9707607.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          411 a          262 c          254 g          389 t
ORIGIN      
        1 tatagaaaag acaccctgaa atcctttaga attaatttgg ctaccaattc ataggaggat
       61 tcaagatgtc tcatactcag cttatcaaag ataccctaaa tattctagac ctaaatatct
      121 atttcgagga aaattgttta actaaagaaa aatacaaagg gcaaatttgc ttgatttaca
      181 aaggaactct tctgtacaag ccagaagagt gttcccattg tctttgtgtc gtcccctcta
      241 ggatcattcg ttgggggacg acaaccgttc ggttgttact gaatgatgtg tctgagtatc
      301 gcacttatct ggagctgaag aaacaacgat ttaaatgtaa gtcttgccaa agaacattcg
      361 tagcggatac ctctgttgct gaaaaacact gtttcattag tcaaaaagtt cgttggtctg
      421 tcattgctcg cttaaaagag aatacttcca tgacggaaat cgctcgtcag aaaaatattt
      481 ccacttcttc ggtctatcgt gtcatgaagc gattctaccg cccattgaat cctttcaaac
      541 agactttgcc aaaggtctta tgctttgatg aattcaaatc ggttcgaagt gttgccagtg
      601 caatgagttt tatcatgatg gacggacaaa gccatgcatt attggatatt gtcgaaaatc
      661 gacgcttgcc ttatttagaa cgctattttt ctcggttttc attagcgact cgtgaagccg
      721 ttcaattgat tgttattgat atgtatgcgc cttatgtttc gttggtgaaa aagcttttcc
      781 cgaatgctca gttgatcatc gatcgtttcc acatcgtcca acatattggc agaactttct
      841 tgaatcatcg agtgaaagaa accacggtac gacttaaagg aaattcccac tctcaacgag
      901 ggttagggaa aaaattgaag cgctattgga aactcctaca aaaagaagaa gcaaagttaa
      961 actatgaaaa acgggtttgg cgtgccagtt ttaaagatta tttgactgaa agtgagattg
     1021 ccgatcgact gttagctgct tgtcccaact tacgccaagg ctatcagtta taccaagaca
     1081 ttctttatgc ggtaaagaaa agagatcagt cactatttga ggattgttta actagagaca
     1141 ttagcggtct gccagaaacc tatacaacca ctttacgaac gtttaaaaag tttctgccac
     1201 agatccaaaa tgcgcttcac tacagctatt caaacggacc attggaatgc ttaaacaacc
     1261 acatcaaagt attaaaacgc aatgcgtacg gctttcgcaa cttttacaat ttcaaa
//
LOCUS       DACDUU010000180         1276 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.187, whole genome shotgun sequence.
ACCESSION   DACDUU010000180 DACDUU010000000
VERSION     DACDUU010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.187"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>1276
                     /locus_tag="IWL30_002919"
     CDS             <1..>1276
                     /locus_tag="IWL30_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HAP9707608.1"
                     /translation="NDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHTC
                     RNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQS
                     YFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSKR
                     ILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEEV
                     KFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKSQ
                     AEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAYP
                     YFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNGK
                     IEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQ"
BASE COUNT          426 a          244 c          246 g          360 t
ORIGIN      
        1 aatgattcta tcaaaaaaat gctgcgatta atagagaaag atttgatgat tacagaggtc
       61 tcttacgaga cctttcagaa gaaaaagacg ttgatcgtcg atgctgtttt ctcacctgct
      121 ccccatacct gtagaaattg tggttctact gtggtagatg gaaacgggaa agtaattgta
      181 gtgaaaaatg ggaagaagga aacgattgtc cgctttgaac aatacaatca catgcctttg
      241 gttatgcgct taaaaaagca gcgctatacc tgtaaaaact gtcgaaccca ttggacggct
      301 caaagttatt ttgttcaacc cagacattca atcgcgaatc atgtcagata taaaattgct
      361 tctttactga ctgaaaaagt atctttatct tttattgcga aaaattgtca ggtatctttg
      421 accaccgtta ttcgtacgtt gaaagagttt aaaagctact taccaaagca atctaagagg
      481 attctcccca gagtattgat ggttgatgaa tttcgttcgc acgcttccat agaagataaa
      541 atgagcttta tttgcgcaga tggcgaaaca ggacaattaa ttgatgtctt gcctacgcgt
      601 aaattgcctc gattaacaag ctatttctta ggctgcgcta atcctgaaga agtaaaattt
      661 ttagtcacag acatgaacgc cgcctacttc cagcttacca aacgtattct gccaaatgcg
      721 aaagtagtga ttgatcggtt tcatattatc aaacacatga atcaagcctt taatgagttg
      781 cgtatccgtg aaatgaatga acttcgtaaa gtaggacaga aaagtcaggc agaaaaactg
      841 aaaaagaact ggcgcttttt gctaaaaaat cgtgcaaaca tcaaccatta tgaatacaaa
      901 acatggaaaa gttttcgagc accaaaatac ccatttctta cggaagcgat gatgattgat
      961 cgattgcttg gcttttctac gtccctgaag gaggcgtatc cctattttca tgaattagtt
     1021 gaagcctttc gagacaaaga ccctgactta tttttctcct tattggcaga acttcccgaa
     1081 acgttagatg acggctttcg ggaaaagctc caaaaccttc tgacctatga agaaggcatc
     1141 accaacgcga tgatctatcc ttacaccaat ggaaaaatag aagcgaagaa tacccatata
     1201 aagacaatga aacgagtatc ctacggattt aaatcatttg agaacatgag aattagaatc
     1261 tttttgatca atcaat
//
LOCUS       DACDUU010000181         1267 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.188, whole genome shotgun sequence.
ACCESSION   DACDUU010000181 DACDUU010000000
VERSION     DACDUU010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.188"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..834)
                     /locus_tag="IWL30_002920"
     CDS             complement(<1..834)
                     /locus_tag="IWL30_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9707609.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EY"
     gene            complement(910..1056)
                     /locus_tag="IWL30_002921"
     CDS             complement(910..1056)
                     /locus_tag="IWL30_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAP9707610.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            complement(1160..>1267)
                     /locus_tag="IWL30_002922"
     CDS             complement(1160..>1267)
                     /locus_tag="IWL30_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP9707611.1"
                     /translation="FTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQF"
BASE COUNT          472 a          197 c          131 g          467 t
ORIGIN      
        1 atattcattt tttatatttt ttaaataacg atattcaaac ttattggtta gataagaatt
       61 agaatagatc tctaattctt ttatttcttt ttccaatatc gaatcatggt tttgattagc
      121 gatttctttt aaactcagca ataaattttt atagtcatgt ctaaatctgg caaatttttc
      181 ttgatctttt tccagtgaat cagtatattt ttttagataa accaattctt ttcttttcaa
      241 ttgctgttca tattgttctc gttgcctaat agttattcta ataaataaaa atagaacaac
      301 aaacatttga atgattaaaa atattaagac accaaacaca aaatgatcaa atacttgata
      361 ttgatgagca acataagaaa caaataaacc aattgtaagt aaataaatca tgaatatagc
      421 agtaagttta gaactggact gttcaacaat cctatttata gctaattttc tatataacca
      481 aataaacatc agcaaaaaca aagaatcaag cattatctgg aatactacat aaccataccc
      541 ttttatccct tcaaatgaaa acatatacgt cataaccatg gaagtaataa tagatctgaa
      601 atcatcaata agcatacata atagtaattt atttaaaagc actatattag ttttcttctt
      661 ttcctttaat ataaaataac ttccgattat aggtatagct gcggaaacat cagtaaataa
      721 ttcaacaaaa aaagtgagtg gtatcaataa acatacaaaa agatattttc ttttgttaag
      781 atcagtatta gtcatataaa ataagagaaa aaagaaaaag tttcccacga acattcttaa
      841 tagataagct tttgtatcga tagtaatcaa gagatcccct cctgatttta ggatatcata
      901 aaaaaaattt tagttacact ttttaaataa tgaaaaaaca cattccacta aatttgatgc
      961 cggttttcct tgaggttttg ttccagcacc tcctttaatt tttttaagct cttgatctga
     1021 acattgttta gcatttagtc tatttttttc ttccattacg attcccccta caaactataa
     1081 atacttttat gtcactgatt caatatacct atttttctaa tcaaaaaatt ttgagacata
     1141 tttaatatta taagtatcat taaaattgag atttatctcc ataatctgct cgatataaac
     1201 catttattcc agcagttctt ccaatttcac tcaaatttct tattaattcc tcttgatatt
     1261 cagtgaa
//
LOCUS       DACDUU010000182         1246 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.189, whole genome shotgun sequence.
ACCESSION   DACDUU010000182 DACDUU010000000
VERSION     DACDUU010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.189"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            200..742
                     /locus_tag="IWL30_002923"
     CDS             200..742
                     /locus_tag="IWL30_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707612.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            832..>1246
                     /locus_tag="IWL30_002924"
     CDS             832..>1246
                     /locus_tag="IWL30_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAP9707613.1"
                     /translation="MDELKRTEIQIIDKKERPKNGFVLSEKTDLENTEAFNKESVKDL
                     RKLPTKDINGKDFTSKDFEKYDLTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIV
                     GVVTDPVDDNGENKEAIEKSKLIHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DACDUU010000183         1234 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.190, whole genome shotgun sequence.
ACCESSION   DACDUU010000183 DACDUU010000000
VERSION     DACDUU010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.190"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            211..996
                     /locus_tag="IWL30_002925"
     CDS             211..996
                     /locus_tag="IWL30_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HAP9707614.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
BASE COUNT          472 a          193 c          190 g          379 t
ORIGIN      
        1 aaataatttt tatattctga cgcatccatt ttactacgga gattatctgc cgcactaaat
       61 aatttttgat ttaattctgc acccaaaaag atacactcct atctcttatt taaagttgct
      121 atataccctc ttattttagc atactccctt ttagaaaaag caaaaaaaaa agaaaaaatc
      181 ttattcttga taacaagcta cgtgacgtat atgcctaaca aaaatagaac ttttgaatca
      241 atcttaaatg aaggtttaac cacagaaccg gcatccatta tttttgatga agttatcgat
      301 gacgttgaag atagtaagta tcataaaaat ttaaaagaac ttttttattt tgcactcaac
      361 aatggaccaa gtttgttcac tggtatatca gtacctgata gtgataatcc attaattatg
      421 gcaaaaattt acttaacaaa atggtgtaat aaatacatca gggataggaa taacccagca
      481 ttaaaaaaac ctcttaaaac atttggcgaa aaagatgctg ctttaactac tagagtagca
      541 gcaaatgcta atattgatga tcaaaaagta ttgaatgact acttgcgtgg acatttttta
      601 tatatgagcg ctgagaacat gaacggaagt attttagagg aatatttagc cgaagtgtta
      661 gaacctgaag gttggatttg gtgcgccggc tctgtatatc gagctgttga tttctgttat
      721 ttaggaacct caccaattct tttacaagtg aaaaataaat acaatacaga aagcagttcg
      781 agttcagcta taagagtagg aacaacaatt agaaaatgga atcgtttaaa taagtcgaca
      841 aaaatttctg gtttggatag ccctatcccc aattggaaag cattaataga aatgactgaa
      901 gctagtaagg aactggcagc taagttaact gaaaatagtt atttagctta tattaatgaa
      961 aaatccacac gagaattatg gacactcgat gactaacaat atccttatta gtatctatcc
     1021 acgatacatg gaaaaaatta taaacaaaca aaaaaacttt gaatttagaa atttctatct
     1081 tacctcagat ccttataata tcttttggat ttacgaaaca atccccacca aagctttaaa
     1141 gtataaattg attgtaaaaa atccaattac taaacttaag aaaaatcaac actacttttt
     1201 aggagacgat gagtttgaac aattagttta aaag
//
LOCUS       DACDUU010000184         1218 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.191, whole genome shotgun sequence.
ACCESSION   DACDUU010000184 DACDUU010000000
VERSION     DACDUU010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.191"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..423
                     /locus_tag="IWL30_002926"
     CDS             <1..423
                     /locus_tag="IWL30_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707615.1"
                     /translation="KNKKNKLSIEQMKKDIETDLKNDHKERRKIEEYTSLMEKCKATD
                     AIFSFLRKSDEIITRENIDLENEISKLESIFSDINSLKYLIVEENSDSFLEFEKYLTD
                     LIDNRNNELMRQNIARFLTKLVSEWKSIISQNMDKLRY"
     gene            694..>1218
                     /locus_tag="IWL30_002927"
     CDS             694..>1218
                     /locus_tag="IWL30_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9707616.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIV"
BASE COUNT          477 a          158 c          207 g          376 t
ORIGIN      
        1 aagaacaaaa agaacaaact cagtatagaa caaatgaaaa aagacattga aactgattta
       61 aaaaatgacc ataaagaaag aagaaaaatt gaagaatata cttctttaat ggaaaagtgt
      121 aaagcaactg atgcaatttt tagcttttta agaaaaagtg atgagataat cacacgtgaa
      181 aatatagatt tagaaaatga gattagtaaa cttgaatcta tcttttccga tataaattct
      241 cttaagtatt taatcgtcga agaaaattcg gatagctttt tagaatttga aaaatatcta
      301 acagatctta tagataatag aaataatgaa ttaatgagac aaaacattgc aagattcctt
      361 actaaattag tttctgaatg gaaaagtatt atttctcaaa atatggataa attaagatat
      421 taaggatgtt agttttaaaa taaactaatg tccttatttt tgtgttcaca tttctgttac
      481 ctaagaaata cttaaaggag gtgatgtagc cagcaacgac tacaacttga acccgctatc
      541 tacagtttat ttgttgtttc ttttgggtat actctaatgg tacaaatcca tatttgctta
      601 aagcgttgat ttttcagcgg tttttcttta ttgcaaagtg taccctaaga cctgtatact
      661 aatagtaaat agattggata ggaatgatgg caaatgtatt tagcttattt aagagtttct
      721 tccgacgatc aaagtttagc tcggcaacat aagctaattg aaaattggtc agtcaaaaat
      781 aatattgctg aagacgattt aaaaattttc gaggaaaaag tctctggaaa aaatattgaa
      841 aacagaccag agctgcaaga gcttatttct ttcatacgtg aacgagatac gattattatt
      901 cctagtcttg atcgattagc aagaaatagt aaagatacaa aagacttact tcaattgttt
      961 cgtagtaaag gtgcttctat tgaaatatta gacctaccgt cgtttaatgg ggtaactgac
     1021 cctgcattaa gagacttgct aacaaattta gtcatagaag tgtttagcta tgttgctgaa
     1081 aacgaacgga aaaaaatcaa agagcgtcaa aaacaaggca ttgaaattgc aaagaaaaat
     1141 ggtcgttata ctggtaaaaa gattcaatac catgccaatg caacaggaaa aaacaaattg
     1201 atttacgatg aaattgtt
//
LOCUS       DACDUU010000185         1181 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.192, whole genome shotgun sequence.
ACCESSION   DACDUU010000185 DACDUU010000000
VERSION     DACDUU010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.192"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..119)
                     /locus_tag="IWL30_002928"
     CDS             complement(<1..119)
                     /locus_tag="IWL30_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9707617.1"
                     /translation="MKKKIILSLSLIMALFLLPINGFAYTINNEFNLGVNEGSS"
     gene            complement(130..327)
                     /locus_tag="IWL30_002929"
     CDS             complement(130..327)
                     /locus_tag="IWL30_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP9707618.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            complement(343..585)
                     /locus_tag="IWL30_002930"
     CDS             complement(343..585)
                     /locus_tag="IWL30_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP9707619.1"
                     /translation="MKEEALQDVVERLVRIETKLDNYESLREKADSAKDLADKAYSVA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            complement(620..757)
                     /locus_tag="IWL30_002931"
     CDS             complement(620..757)
                     /locus_tag="IWL30_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP9707620.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADRIISKEESA
                     S"
     gene            complement(759..1142)
                     /locus_tag="IWL30_002932"
     CDS             complement(759..1142)
                     /locus_tag="IWL30_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707621.1"
                     /translation="MLVSVPYTDQPPLEGVRSDGEPLTIADQMFDPKLNQWIILANAL
                     DHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIKQENTALKEKADSLAQINSKMM
                     LASLQNSKDISEIKEQLNPASKGGE"
BASE COUNT          317 a          258 c          186 g          420 t
ORIGIN      
        1 gagctacctt catttacacc taaattaaat tcattgttga tcgtataggc gaacccgtta
       61 attggcaata aaaaaagagc cattattagg ctcaatgata aaatgatttt cttcttcatt
      121 tttttcctcc tattttttta agttatatgc agacacacca gtgataacgc ctaaaaatgt
      181 tgctactgca ttgatagtga gtactgtcat atctgttcca ttccatccat acgcttttcc
      241 taacgtggct actaaaacag atgcagctgg taatactgtt aaaaccgtcc atttaatgac
      301 ttgataatac ttatcgggta aaatcatttc ttcttaactc ctttacaatt tagtcaagaa
      361 atagccaatg atcgtaatgc ctaaaccgat catgtaaccc cacgaccatt tattattggc
      421 tttcatttct ttgatatctt ctgcgttgtt tagcgctact gagtaggctt tgtctgccaa
      481 atcttttgca ctatcagctt tttctctaag tgattcgtag ttgtctaatt ttgtttcaat
      541 tcttactaat ctctccacaa cgtcttggag cgcttcttct ttcatgttcc accaactttc
      601 caacaaaaaa acgcatcact taagatgcgc tctcttcttt gctaataatt ctatctgctt
      661 cttcatctgt gatacatagt ggaacgaata gacgaacttg atcgtcagta aaacagcccc
      721 aatcatacat cattttcaca tcgctaaaac taaacatact actcacctcc ctttgaagct
      781 ggatttagtt gctctttaat ttctgaaatg tctttgctat tttgtaacga agcaagcatc
      841 atttttgaat tgatttgtgc taaactgtca gctttttctt tcaatgcagt attttcctgt
      901 ttaatcgcta catcgcttag catgagtttg gcatttagct gttttaggtt gccgttttca
      961 tgttcaagag cctcgtacat cgctttgaga ttgtttaaat cgttgtgatc taacgcgttc
     1021 gctaaaataa tccattggtt caatttagga tcaaacatct gatcagcaat cgttagcggt
     1081 tcgccatctg aacgaacccc ttcgagcggt ggctgatctg tgtaaggaac ggatacaagc
     1141 atgtcgtcca atacttttcc tgcatactct ccgccagtac g
//
LOCUS       DACDUU010000186         1137 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.193, whole genome shotgun sequence.
ACCESSION   DACDUU010000186 DACDUU010000000
VERSION     DACDUU010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.193"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..355
                     /locus_tag="IWL30_002933"
     CDS             <1..355
                     /locus_tag="IWL30_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707622.1"
                     /translation="GAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETNMTRDELL
                     RKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTTSQIKQDT
                     EDSLRKGWGMNNKVR"
     gene            615..985
                     /locus_tag="IWL30_002934"
                     /pseudo
     CDS             615..985
                     /locus_tag="IWL30_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            985..>1137
                     /locus_tag="IWL30_002935"
     CDS             985..>1137
                     /locus_tag="IWL30_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9707623.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
BASE COUNT          431 a          135 c          237 g          334 t
ORIGIN      
        1 tggcgcacca atcagaaagt ttgataaggt ttattccacc aagcatcctt taaaactgtc
       61 gactttgtta aatgcgactt ccagagtttt agtagaaaag tatgagacta atatgacaag
      121 agatgaattg cttagaaaag ctttagatga gtatattaaa cagaatctta cgcaagaaga
      181 taagcaagat ttgtttaatg acgtggtgaa agaactagac ttgttcagag aaaaatatcc
      241 aattgttcca gaattagatg atcgtggaga aataataaga actaccagtc agataaaaca
      301 agataccgaa gactcactgc gcaaagggtg gggaatgaat aataaagtta gataataatc
      361 aaagaagaac taagtaacta cctttaaacg gtagttactt agttcttctt tgatttgcat
      421 gaaatagagg ggatttattt ttctggttac gtgattaaaa aatccaaaaa atgagcatag
      481 gaacggttat gattttaggg tgtgttttaa tgagatattg tgatttcatt aacaagtgta
      541 tgataatctg ttgttgagtt agtaaataag taatagagct aactctaaaa taaaactatt
      601 aggaggagat gtaggtgaaa aagtttaaaa agaaaatata tttaggattg gttgttgggg
      661 tgagtatgtt tgggttcatg gctcatacta aacaagtaac tgcagatact aaccaaaaag
      721 aaaggattat gagccaatta gatgactctc aaataagtat gtacccaatg agctaattgt
      781 cagtttaaag gctacacaaa gaatttcaac agagaaaaat aaaccgcaaa atttattggg
      841 tgtaaaagtt atggtggaaa aagatttgac tcttaggaca aatgaaagcg attgtaataa
      901 gcgagatcaa gggttctgtt gcaaagtttt ccaaaaaatc tattttagtg taaaattgag
      961 aaaaaagaca gagaggacag agtaatgaat cattttaaag gcaaacaatt caaaaaagac
     1021 gtcattattg tcgctgttgg ttactacctg cgttacaatc taagctatcg tgaagttcag
     1081 gaattgttat atgatcgtgg aataaatgtt tgtcatacta cgatttatcg ttgggta
//
LOCUS       DACDUU010000187         1117 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.194, whole genome shotgun sequence.
ACCESSION   DACDUU010000187 DACDUU010000000
VERSION     DACDUU010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.194"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            154..417
                     /locus_tag="IWL30_002936"
     CDS             154..417
                     /locus_tag="IWL30_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9707624.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            417..689
                     /locus_tag="IWL30_002937"
     CDS             417..689
                     /locus_tag="IWL30_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP9707625.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            766..1095
                     /locus_tag="IWL30_002938"
     CDS             766..1095
                     /locus_tag="IWL30_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9707626.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT          424 a          142 c          221 g          330 t
ORIGIN      
        1 ctctaggaaa aaattaacta gcaccacggg tataaaggaa acggataaca ctttacaatg
       61 ctgttttggt actccaattt aaacaaaaca atcgaattta cgtacttaat taagtacgtt
      121 agaatggaga tataaattag agggggagta gatatggcaa aaacagtctt aaatcctagc
      181 tcagcacgga aaaattttta taagttgttg aaagatgtaa atgagaatca tacggaaatt
      241 gaaatcatta gtgagcgcag tgaaaataat gcggtgctta ttggtttgga tgattggaaa
      301 gcaatcaaag aaacgctatt acttgagcaa ataggcactt tgggtgttgt tcgtaagcga
      361 gaaaaagatg atagtggatt tactaatgtt gatgaaattg attgggataa cttataatga
      421 ataactattc tgtgatgatt aaaaactcgg ctaaagctga tttaaagaaa atcaaacagt
      481 ctaatttgaa acctcaattt gagaaggtta ttcagacttt aaaggaagat ccctatctcc
      541 ctacccaatc atttgagaag ttaaaaccta cacatgaagg taggtattct agaagattga
      601 atagacaaca ccgggttgtt tataaggtag atgaagatga acacgtggtt gaaatttatt
      661 cagcaaggac gcattacgag tcacagtaat tacttgtaaa tacattatct cgtggtataa
      721 tgtatttact aagtaattac atactgttag aaatgaggga tttaaatgat tacaaaaaca
      781 agaaaacaag gcaattcaat catgttgaca gtacctaaag attttaatgt tcctaatgga
      841 gtcgaagttg aagcaaagtt ggtagagaat ggtattcttt atgaatttgt tgaaccacaa
      901 aaagaatttt atgattttag cgaagatatt ttgtctgata taattgcaga aggctacgat
      961 aaagatgaga ttcttgtaga atttaagaat agaaaaaaca aaatgcattc ttcctttaga
     1021 gatattgctg aagatacact tactaattct aaagtaatga ctaaagagga gttggcaaca
     1081 gaaattggct tatgaaatcg ttccttcaaa acatgtc
//
LOCUS       DACDUU010000188         1108 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.195, whole genome shotgun sequence.
ACCESSION   DACDUU010000188 DACDUU010000000
VERSION     DACDUU010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.195"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            78..989
                     /locus_tag="IWL30_002939"
                     /pseudo
     CDS             78..989
                     /locus_tag="IWL30_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
BASE COUNT          297 a          193 c          254 g          364 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttacgta gctatcagca ccttttcgat ccaacaattc attaataact
      121 tttctagcga tatcatattg aatgcgaatg ggcagccgat cccgttttct agtcaagtat
      181 tgagttgcat catggtgatg ctctgggtat tcgttggtat tgaaggagct tctgtcgtag
      241 gttcacgagc aaaaaaacaa tcggatgttg ggaaagcaac gattctagga atattcggtt
      301 tgatcattat ttacgtcttg gtttccatgc ttccatatgg cacaatgacg atgaatcagc
      361 tgcaaacgat caaagcgcaa cctgctatgg gatatgtatt tgaaatgatg gttggtaaat
      421 ggggggcggt cgtgatcaac ggcggtttga tcatttcttt agttggggtt tggctctcat
      481 ggacaatctt gccgattgaa acaatgggga atatggctga ggacggactt ttgcctaaag
      541 catggggaaa actcaacaaa aatggggcac caacttatgc tatcgtattg acgacgttat
      601 gtacgaatgt ctttttactt tcattattat tcactgacag tgcatataac tttgcttata
      661 cacttggtac agcagcgatt ttcttcacgt ggcttttctt agggctgtac caaatgaagc
      721 tttcttatca gcggaaagag tgggtccaat ttttagtagg gtttttagca agtgcttttc
      781 aagtatgggc catgctagtt gtagccttta atgaagtgat gcttgttctt gttcttttct
      841 taccaggtat ttatttttac ctcaaagcac gtaaagaaca aaacgaacct gtacaacata
      901 tccacttaga taagatttta cttatcggtg tgacgatggt cggagtaatc gcaatggttt
      961 tatttgccac tgggatgctt cacgtataga agtataaaga gaggctgtga agcctgtggt
     1021 ttattcaaaa aagaagcggt gacaacattc gtgtcatcgc ttcttttttt gaataaatgg
     1081 tgttcaagaa gtagcttctt ctaaaaat
//
LOCUS       DACDUU010000189         1099 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.196, whole genome shotgun sequence.
ACCESSION   DACDUU010000189 DACDUU010000000
VERSION     DACDUU010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.196"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            134..>1099
                     /locus_tag="IWL30_002940"
     CDS             134..>1099
                     /locus_tag="IWL30_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP9707627.1"
                     /translation="MNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAFHINR
                     ALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLYIVTL
                     ITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPLILLG
                     FIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIFLIIK
                     LIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLS
                     FGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWF"
BASE COUNT          373 a          136 c          146 g          444 t
ORIGIN      
        1 tgaaacacta cttttgtaat ataaaaaata ttttattaat aataaaatta atcacatttt
       61 atatagtgac taatctagtg ttttaaaaaa ataaatgata ctatatgtaa tataaaaaaa
      121 catgaggtgg tatatgaatt tgaaaaaaat tttttctgta aaaatattta tagtttatgc
      181 atttgttagt ttattatatt tatttccagc atttaaaaac atggaaatat ttggctttta
      241 tgatacggca tttcacatta atcgtgcgtt aagtttagaa tctattttta gctcccctat
      301 taattttgaa acgtttagat catatggcat gcaagtaaat aatttttacc catggttaac
      361 tctatatcct ttatttttat tgatcaagtt tactaattta gcaataggct ataacctttt
      421 tctttatatt gttacgctca ttactctatt tatatgtcat tatgtaatgt atgaaattac
      481 caaaaaacat gttactagct cctttttcgc tattatatat acaacatctt cttttaggag
      541 tgtagaaata tttcttcgag gggcaatggg ggaattactc gcaatgtcaa tacttcctct
      601 tattttactc gggtttataa agctttacga ctcaaaaaaa gaatcttggg ttatgttggc
      661 gattagcatg actttattaa tttacactca tgttctttct gtagctatga ctatggtaat
      721 gattataatt gctttaatta ttcaatttta tcggaaaaag aaaatagaaa tcttcttaat
      781 tattaaatta ataaaagctg ctatagttac tttacttctt tcactagcgt ttataggtcc
      841 tatgattgag cagacattgt atcaagatct gaatagacct tatgtggata tattacaaaa
      901 aagagcaatt tatctgggta aagagttttt gattggaagt atagattcag aattactatc
      961 atttggtatt ggcttaactt tgcttatttg tttaattata ctaacattta actttaaaaa
     1021 attaggaatc ttatctaaaa tcacttatat aatgggtata atatctattc tactttgtac
     1081 aaaaatattt ccatggttt
//
LOCUS       DACDUU010000190         1082 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.197, whole genome shotgun sequence.
ACCESSION   DACDUU010000190 DACDUU010000000
VERSION     DACDUU010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.197"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(73..344)
                     /locus_tag="IWL30_002941"
                     /pseudo
     CDS             complement(73..344)
                     /locus_tag="IWL30_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876065.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
     gene            455..>1082
                     /locus_tag="IWL30_002942"
     CDS             455..>1082
                     /locus_tag="IWL30_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HAP9707628.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLK
                     IHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          373 a          196 c          171 g          342 t
ORIGIN      
        1 ggcaaaaatt tcaatcttta aatggcgaaa aacaaagagc gtgagaccct atccttatct
       61 cacgctcttt gtctattttt ctaacaaatc aagtatttct tgaggagaat caataattat
      121 atctacactt tgaaaatttt ctatattttt ggctccccat ttagctaaac caaatgctat
      181 cccacaagct ttcgcacttt gcatgtcata taatgaatca ccaatataaa tcgtttctta
      241 ttttttatgc ccaattcatc aattgccttt tgtatggggt ccggaaaagg tttatgcaac
      301 ttagtatctg aagctgtaat ttgtatatca aaatatagat ttaacccgtg gtgctagtta
      361 attttttcct agaggaaaat gtctaaacat gttggtaaat caacaaaaaa acgcatatag
      421 tagtctcaaa actcccaaga aatgaggact acatatgcgt tcatcaaaat atactgaaca
      481 ttatacagat acttttataa cattcaagga aattatgaga actgtttcta acatatacca
      541 caattgtgta ccagataaga ttaaaaaccg aagaaatact gatcaactga agcaacgcga
      601 tacagtgatt attgcttgtg ttatatgggg cataattaat ggctatacta gccaaagagc
      661 cacgtataga gcggtttgtt ccgtcttatt tcctaatggt gactttccta gtaggagtcg
      721 attcactcgc ttaagttcaa acttagctta cactatcaag attattcgat actttttcat
      781 caagaagctc accaaaggtg aactagtcgg aattatagat agttttccta gtccattatg
      841 taaaccagtt agaaatagac aagcaaaact attgaatcaa atagccaaag ttggctataa
      901 ctcaacaaaa aaatcttatt tttatggtct taaaattcat atgatcgtta ctaagactgg
      961 ctttccgatt acttactcaa tcacgaatcc aggtgtccat gacgtgaaag tgttagagac
     1021 tttatcggaa gaagcgaatc ttcccaatat cctaggggac aaaggatata ttagccataa
     1081 aa
//
LOCUS       DACDUU010000191         1070 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.198, whole genome shotgun sequence.
ACCESSION   DACDUU010000191 DACDUU010000000
VERSION     DACDUU010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.198"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(1..322)
                     /locus_tag="IWL30_002943"
                     /pseudo
     CDS             complement(1..322)
                     /locus_tag="IWL30_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            398..553
                     /locus_tag="IWL30_002944"
                     /pseudo
     CDS             398..553
                     /locus_tag="IWL30_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
     gene            572..>1070
                     /locus_tag="IWL30_002945"
     CDS             572..>1070
                     /locus_tag="IWL30_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9707629.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKL"
BASE COUNT          386 a          128 c          192 g          364 t
ORIGIN      
        1 gatatggaat atttatgctt gttggcatca attacttgtc ttttcgtccg aatatcagcg
       61 ccgcttgctt taaaatatca ttctccattt cgagttgctt atttttcttt cttagtgcga
      121 tcaattctgc ttgttctggt gttaagttgt ctctttcttt gaatgagccc gttgattgtg
      181 cttgcttcat ccatttatcg aaagaagaag gcgtaagctc atactctcga ataatttctg
      241 cgcgaggctt accagcgaga tagagatcga cgatttgttg tttgaattct tttgaatagg
      301 ttcttcgttg acgtcttggc atgaaaaatc ctccagtgtg ttttttatta ttctacacac
      361 cttatttttt ctgtctagtc tagtgtagcc gattcaggat aaggataagt gtattaattg
      421 tggaaaatgt aaaaaagttt gccaaatgaa taaagattca gttgagaatt gcaatcatct
      481 ggagtgtatc agatgtggac gatgtaaaaa tgcttgtcca gtggatgcaa ttagttatgg
      541 agtaagaaaa tagagaggag tagttaggaa agtgataaaa aatgctatag catatattac
      601 aagaaagaaa aatagaactt taataatttt tatcatatta actatagttc tttcttgctt
      661 gtattcttgt ttaacaataa tgaaatcaag caatgaaata gaaaaggctt tatatgaaag
      721 ttctaattct tcaatatcaa taacaaaaaa agatggtaaa tattttaatg ttaatcaatt
      781 taaagatatt gaaaaattaa aagaaattga agaaaaaata attcaatatg atggattagc
      841 aaaactaaaa gatgctaaag tagttagcgg agaacaaaga ataaatagag aagatttatc
      901 tgacgaattt aagaatgttg tttcactcga agctacaaat aatactaaaa gaaatatttt
      961 atttagtagt ggagtattta caattaaaga aggtaaaaat atagaagaaa atgataagaa
     1021 ttcaattatt gttcatgaag aatttgctaa acaaaacaat ctaaaattag
//
LOCUS       DACDUU010000192         1062 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.199, whole genome shotgun sequence.
ACCESSION   DACDUU010000192 DACDUU010000000
VERSION     DACDUU010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.199"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(37..993)
                     /locus_tag="IWL30_002946"
     CDS             complement(37..993)
                     /locus_tag="IWL30_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707630.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          379 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 ttttataatt ataggtagcg tatacaattc cacaaaactt atctctgttg agttaaaaag
     1021 attatataaa aaaagccgaa tgtttttagg attccctgtc aa
//
LOCUS       DACDUU010000193         1062 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.200, whole genome shotgun sequence.
ACCESSION   DACDUU010000193 DACDUU010000000
VERSION     DACDUU010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.200"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            288..695
                     /locus_tag="IWL30_002947"
     CDS             288..695
                     /locus_tag="IWL30_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707631.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            709..>1062
                     /locus_tag="IWL30_002948"
     CDS             709..>1062
                     /locus_tag="IWL30_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707632.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLS"
BASE COUNT          395 a          134 c          181 g          352 t
ORIGIN      
        1 ttgaggggaa gtacatatag ttgttgatat agttgctaat attgttagct aatttgttag
       61 taaaactgta tacgaattta gatagtaaaa aaacagtttg ttccttatat agaggcactt
      121 ttactactga ttaaatgtca gatatgctta caaatatact tataaatatg cttacaaaat
      181 tagctaatat ttccgttgac aaagctttgt tactattgtt aaactaaatt gactagcaaa
      241 agtgaaacat aaatttatta aataaatgta aaaattagga gaaatcaatg tcttatagag
      301 tacaattaat aatttcagaa gatgtagaaa gtcagcaatt tggcactaat gttattaata
      361 aagtaattaa tccagctctt tctattaatg cgcctttaat tcctactgct ttatcatttt
      421 ctgtaacagc tgtggtgtct gagatagagg atacagaaaa gataaaaata gtagaaattg
      481 aagttttaaa caagaatgaa aaacaaattt tttctacggg tgaagtatcg gtaagcttgc
      541 ctcctcaagt taacgatata aactttaata taaacgccag aaatgtcttg gttgaagaag
      601 caggagagca ttatgctgtt gttaaattta atggaactga gattggaagg caaatctttg
      661 atatcaaggt caacaaacca gtggaaaaaa attaaggaga cgatacggat gcttgatata
      721 gtaccatcaa atacacatcg gagtggtagc ttaataaatt ttgctgcgat ttttgcttgc
      781 gcatcggtaa ttctagcatc tccagttgtt gtggatgcaa gttctactcc gaaaactaaa
      841 aatgataatc aacttgtaat tactacaaaa aatgagatta acacaatttc aagtaatgat
      901 ggcaaaattt ttgatgtgat aaatacggtt ttaaaaagaa aagctgaaat caatataaaa
      961 tatgatgaag atttaaactt gtatttcttt gttatcaaaa caacatctga attgttttcc
     1021 tcagattacg atgttttgga tactctcgat aatgtattat ct
//
LOCUS       DACDUU010000194         1030 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.201, whole genome shotgun sequence.
ACCESSION   DACDUU010000194 DACDUU010000000
VERSION     DACDUU010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.201"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            169..405
                     /locus_tag="IWL30_002949"
     CDS             169..405
                     /locus_tag="IWL30_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAP9707633.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            424..639
                     /locus_tag="IWL30_002950"
     CDS             424..639
                     /locus_tag="IWL30_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAP9707634.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
BASE COUNT          334 a          139 c          237 g          320 t
ORIGIN      
        1 aaaaactttg aaaacagttc ttgaacacta actttttcat tcctgaatca aatttatttt
       61 ggaaatttta ctattcaaga ataaacttga taagtttcag gaagattttt actgttaatc
      121 aaatacgtat gttacagtag caccgtaaaa aatattattt aaagaagcgt gtttaaacat
      181 gcaaaatgta aaggaactaa gtattgtgga aatgcacaaa ctgttggtgg aggcaataat
      241 gcttggggaa aattggggca agttgtaggt ggacttacca caggggcagt aggtggtgca
      301 ggtttaggaa cagcaatttg cggaccagct tgcggtgttg ttggagggtt atatggagct
      361 gtcgctggag gtgctgcagc tggctgggat gctcgaaaaa aataaaaaag gagaataagt
      421 agtatgcata ttaaaaacac aaaaacaaca tttatattat cttcagaaga gttgaaaaat
      481 attcaaggtg ggagtgccgt gggagtttta ggcactactt tttctggggc aacagcagga
      541 gtaaaacttt gttcagccgg aggaccttat gcaatagctg cttgtggtgt aggtggagct
      601 ttattaggtg ctggattcag catgtggacc ggtgcgtaaa caattaaaaa atatcaaacc
      661 tcaagattgg tttgtgtcga ttacgaacat aggtatgttc tttatctttt caaaagtttg
      721 gaatggacag aactttttac taaagcttct tgtgctagtt tttataatta tattaaattt
      781 ttatctttta ttaaagtcag ataaaaattc tttggaaatg taatggtaga gatattttta
      841 caaaaggact tgcagcgctg tagttggtgc tgcaggagga cctatttcgg ccgttacagg
      901 aggtattggt aatgtacttg tcggatgtgc attaaagcct gttcctgcaa gttaaattta
      961 taaaaacagg aaagtaatta tctcaaatcc tatcaagtac aatcatagga ttgcacttga
     1021 caggacaaat
//
LOCUS       DACDUU010000195         1002 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.203, whole genome shotgun sequence.
ACCESSION   DACDUU010000195 DACDUU010000000
VERSION     DACDUU010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.203"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            369..1002
                     /locus_tag="IWL30_002951"
                     /pseudo
     CDS             369..1002
                     /locus_tag="IWL30_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          182 c          183 g          295 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata
      301 taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt
      361 ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa
      421 ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata
      481 aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac
      541 gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat
      601 atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg
      661 cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt
      721 ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag
      781 tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca
      841 aaacatggcg cagttttcgt gcacctaaat acccgctact cactgaagca atgatgattg
      901 atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga
      961 ccgaagcctt tagagacaag gaatcagact tattttttac ct
//
LOCUS       DACDUU010000196          985 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.204, whole genome shotgun sequence.
ACCESSION   DACDUU010000196 DACDUU010000000
VERSION     DACDUU010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.204"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            137..>985
                     /locus_tag="IWL30_002952"
     CDS             137..>985
                     /locus_tag="IWL30_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAP9707635.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNV"
BASE COUNT          421 a          150 c          162 g          252 t
ORIGIN      
        1 ctcgtgaaaa ctatgtaaat gttctccatc atagagagaa tagattttta cgaactaaga
       61 atatctacag caaccttacg aaaatgaaag actgtctccc taattcttct gttagactgt
      121 ttttaataag gaaaggatgg gaaatttttt gaaaaaacaa ctactaatta tgtgcctttc
      181 agcatgtgca attttactat tctcaggttg tagcaagtcc aatgaaacga ttgcaacgtt
      241 taaaggtgga acacttacag ttcaggattt ttatgataac ccaaaagtta aatcaaataa
      301 tcaaacacta ttaaaaaaaa tgattgtctt caaagcattt gatgatgtat acggaaaaga
      361 gatttcttca aaggaagtaa caaaagaata taatgaacaa atcaaaaaac tcggtccaaa
      421 ctacaaggaa caattaaaag cagttggcca aacagaagag acatataaga aactctttaa
      481 acagatgctg gctttccaat acggtttgaa agccaatgta aaattaacag ataaagatct
      541 agacactgct tggaaagaat tctatccaga agttagtaca cagatcattc tattttcgac
      601 taaagaagaa gcagataaag caaaaaaaga agcaaatgaa ggagaaaatt tctccaagct
      661 tgttcaagct tatggaaaaa ataaaacatt aaaggaaact gatggaaaaa tgaattttga
      721 ttcaaccaat ccagagattc ctactgaagt aaaaaaagca gcatttaaac taaaaaacgg
      781 ggaagtttct gatatcattc ctgtaacaga tccaacgact tatcagcaaa gctattatct
      841 agtaaaaatg gtaaaaaaac aggataaagg ttctaataaa gacaaataca aatctgagct
      901 agaaaaaatt gctaccgaag caaaaactct tgatacggaa ttcatggata aaacaatccg
      961 taaagtgatg aaaaaagaca atgta
//
LOCUS       DACDUU010000197          983 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.205, whole genome shotgun sequence.
ACCESSION   DACDUU010000197 DACDUU010000000
VERSION     DACDUU010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.205"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            48..>983
                     /locus_tag="IWL30_002953"
     CDS             48..>983
                     /locus_tag="IWL30_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9707636.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTK"
BASE COUNT          354 a          179 c          186 g          264 t
ORIGIN      
        1 tacactcaaa atagttccga cgaaagaatt ataaggagtg agtacaaatg acctataacc
       61 atcttacacc aactgaactc gtaatgatag aagcttattt caatcaatca caacctgttt
      121 caaaagtagc caatctgtta caacgttctc gacaaactat ttataaggtc tatcgttttc
      181 ttaaatctgg tggaagcgca atgagctact ataaacaata taagaaaaac aaacgtaact
      241 gcggaagaca tccgattgtt ttacctgatg agcaacagga atacattcaa aaaaaggttg
      301 ttcaagggtg ggctcctgat gtccttattg gtcgagcaga gtttcctatt gcttgttcgg
      361 tacgcacact gtatcgaatg tttaaagata aaacctttga cactcacgat ttaccaatga
      421 agggaaaaag aaaaccaaac ggtcataaag aaaaaagagg aaaacaaggg tttcgccggt
      481 caatccgaga cagaaagaca gactataccc agtttgatca tgaatttggt cacctggaag
      541 gtgacactat cgtgggtgga aaacataaaa gcgcggtcat cacattagtt gaacggctat
      601 ctaaagtcat cattaccttg aaacccgaag gcagacaggc taaagacatt gaaaataggc
      661 tgaatcaatg gtttcgttgt gtcccaaaaa acttatttaa atccatcaca tttgattgtg
      721 gaaaagaatt ctcaaattgg aaaaagatta gtaataccaa cgatatttca atttattttg
      781 cggatccagg aacaccttct caacgaggat tgaatgaaca ttcaaatggc ttattacgta
      841 aagatggttt gtataaagct atggatttta atggtgtatc tgaaaccttt atccaatcgg
      901 tggcatcgaa acgaaatcat attcctagaa aatcattaca ctatcggaca ccaatggaag
      961 tatttttgag ttacgtcaca aaa
//
LOCUS       DACDUU010000198          968 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.206, whole genome shotgun sequence.
ACCESSION   DACDUU010000198 DACDUU010000000
VERSION     DACDUU010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.206"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>968
                     /locus_tag="IWL30_002954"
     CDS             <1..>968
                     /locus_tag="IWL30_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP9707637.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDG"
BASE COUNT          313 a          172 c          214 g          269 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggt
//
LOCUS       DACDUU010000199          967 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.207, whole genome shotgun sequence.
ACCESSION   DACDUU010000199 DACDUU010000000
VERSION     DACDUU010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.207"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..461)
                     /locus_tag="IWL30_002955"
     CDS             complement(<1..461)
                     /locus_tag="IWL30_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9707638.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYS"
     gene            complement(746..952)
                     /locus_tag="IWL30_002956"
     CDS             complement(746..952)
                     /locus_tag="IWL30_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP9707639.1"
                     /translation="MNLIYDEIAIDPYKGEQKTGDLSGIWSSGFKYAGTTYRVAYEII
                     ENKVIPVLLCGTHENFYEQLKKLI"
BASE COUNT          313 a          184 c          128 g          342 t
ORIGIN      
        1 ttactatata cagaaataga aggtgggaca tctaaataga tgacgtcaaa ctcatcaaat
       61 aatggttcta gcttttcttt catatatttg aattgggata atgaatcatt atattggtct
      121 ttgatccaat catcatacat ttcaaaggac atgtctgctg gaagtatata gaggttattg
      181 attactttat agacagcctc tttaggtgga agattgttta tcaacacgtc agtcacagta
      241 ttttcgtaaa caatcggttc accttctccg cctccttctt cgttagtgaa gttcatatgc
      301 gttaagttca tcacatcaga agtatttcct tgcatatcca agtcaattat caaggttctt
      361 tttccttcat taactgctga ttcaaaccct aacatttgcg cgcacgtact tttacctacg
      421 ccacctttaa aattaccgat tacatatttc ttcgctacca cgttcctacc tcttttcttt
      481 tagcaatatt catttgatac tattataaca tatttatttt tagttactac caaaaaaaag
      541 atatatatgt actaccttat aattaataag taaaaggtag tacataaata aggaaaagct
      601 gaaatatatt gatttatagc tttcctcaaa gtaatgacgt tatttaattt atcgagaagc
      661 agagaatact cgcgacttta gtcatgtaga cgaatcagat tttcgataaa aagaagccgt
      721 ccttcttaag aacgacaaca aatcactaga ttaatttttt caactgttca taaaagtttt
      781 catgcgttcc acataacaat actggaataa ccttgttttc tataatctcg taagctactc
      841 tatatgttgt gcccgcatat ttgaacccac tagaccaaat acctgataaa tccccagttt
      901 tttgttcacc cttatatgga tcaatcgcaa tttcatcata aattaaattc aaaaattttt
      961 ctttcaa
//
LOCUS       DACDUU010000200          943 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.208, whole genome shotgun sequence.
ACCESSION   DACDUU010000200 DACDUU010000000
VERSION     DACDUU010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.208"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            2..>943
                     /locus_tag="IWL30_002957"
     rRNA            2..>943
                     /locus_tag="IWL30_002957"
                     /product="23S ribosomal RNA"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DACDUU010000201          935 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.209, whole genome shotgun sequence.
ACCESSION   DACDUU010000201 DACDUU010000000
VERSION     DACDUU010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.209"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..818
                     /locus_tag="IWL30_002958"
     CDS             <1..818
                     /locus_tag="IWL30_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP9707640.1"
                     /translation="TRYQLIKQLIRTKQHFLENLTYKCNTLAREMRDESTSLFSATLI
                     SLMTEDFTLDELAELPLEAFCDLLQEKGKGRFKQPEKIAKAIQRAITMSYRLGALAQE
                     SINVVLSVLVREIRALEKNIKELDKAIEQVIVVIPEYQCLTSIPGVGKVYAAGLIAEI
                     GQIERFEDQTKLAKYAGLSWKVNQSGNYQSQNTPLTKQGNRYFRYYLVEAANSVKNYL
                     PEYKAFYQSKYKEVPKHQHKRALVLTARKFTRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          334 a          182 c          163 g          256 t
ORIGIN      
        1 gtactcgcta tcaacttatt aagcaattga ttcgtacaaa gcaacatttt cttgaaaatc
       61 taacgtacaa atgtaatacg cttgctcgag aaatgcgaga cgaatcgacg agtcttttta
      121 gtgcaaccct catttcactc atgacggaag atttcacatt ggatgaactt gctgagcttc
      181 ctttagaagc tttctgtgat ttacttcaag aaaaaggaaa aggccgtttc aagcaaccag
      241 aaaaaatcgc taaagcaatt caacgtgcaa ttaccatgag ttaccgccta ggtgctcttg
      301 cacaagaatc aattaatgtt gttttaagtg ttctcgtacg agaaatacga gcacttgaaa
      361 aaaatatcaa agaattagat aaagccatcg aacaagtaat tgttgtcatt ccagaatatc
      421 aatgcttaac cagtattcca ggcgttggta aagtctatgc tgccggttta atcgctgaaa
      481 ttggtcaaat cgaaagattt gaagatcaga caaaattggc aaaatacgct ggattaagtt
      541 ggaaagtgaa ccaatctgga aactatcagt cgcaaaatac acctctgaca aaacaaggaa
      601 atcgatattt tagatactac ttagttgaag ctgccaactc tgtaaaaaat tatcttcctg
      661 aatacaaagc tttttatcaa agtaaatata aggaggttcc aaagcatcaa cacaaacgtg
      721 cactcgtcct aaccgcaaga aaatttacgc gtctggtgga tacgctacta cgtaaacacc
      781 aactctatac gccaccaagg agtgtgatag agaaataaca atccgttatt gacgcaaatc
      841 cttgaatcaa cccgaaaaaa atttgatttt gatcgggtct agtttcgtgc gcttcaaaac
      901 atattactca ataaagaaaa tcttaaattt catct
//
LOCUS       DACDUU010000202          912 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.210, whole genome shotgun sequence.
ACCESSION   DACDUU010000202 DACDUU010000000
VERSION     DACDUU010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.210"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
BASE COUNT          319 a          115 c          173 g          305 t
ORIGIN      
        1 agaatgatgg aatataagga taaggttacc aacataacga tcattacttc tctgatatgc
       61 tatggacttc tatcaatcag tattttgtct atagattatg gagtaactat actgcaagcc
      121 ttagttttgg ctatgcctat gtatctatct ttgtttatca tgcatgaaat atataagcta
      181 tttgttttat caaaaaatgt agaaatacgg gtaaatattt tggtgaatag ttatcaaaaa
      241 gaactcaaag aaagaagacg gtggcggtat ttattgaaag gatgaaaaag taggatttga
      301 catactggta ttcagaaagt gccgtcactt ttaaaacaat ggaaaaggtg gagataagat
      361 gaagaaaaaa caaactaaac tatcattagc agatatttaa cacagttaac cgcaactgaa
      421 gatggtgttg ttgaatttga acaatctgaa attgcggtag tagatgatcg ttaccttaaa
      481 atgccttact tctttgacca agcaaaggtc atttgccttt gtcactacga tggggtacgt
      541 gattttaggc gttaaaaagt agtgattata tcaactttac gagtaaatat aaatttagac
      601 atgaaactga attatccaat ggaaaatcat ggtttataat ctagaaaaat tatagtaatg
      661 attggattat atatattctt tcgtattttc gtacaactgt agcttgtcaa aattgagaaa
      721 tttaaccgat taggtacttt acatcgttat ttgggtaatt tctaaaacat gaagcttacc
      781 aacgaataaa ctgagactat cttattaatt gatcaatata agaaaatatc tttgaggaga
      841 tgatgtacta tgttttcgtc tatccacgat tggttaggtg gtcttttcgg cggtggaggt
      901 aaagtttttt aa
//
LOCUS       DACDUU010000203          855 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.212, whole genome shotgun sequence.
ACCESSION   DACDUU010000203 DACDUU010000000
VERSION     DACDUU010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.212"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..>855)
                     /locus_tag="IWL30_002959"
     rRNA            complement(<1..>855)
                     /locus_tag="IWL30_002959"
                     /product="16S ribosomal RNA"
BASE COUNT          177 a          265 c          188 g          225 t
ORIGIN      
        1 ctctagagtg gtcaaaggat gtcaagacct ggtaaggttc ttcgcgttgc ttcgaattaa
       61 accacatgct ccaccgcttg tgcgggcccc cgtcaattcc tttgagtttc aaccttgcgg
      121 tcgtactccc caggcggagt gcttaatgcg ttagctgcag cactgaaggg cggaaaccct
      181 ccaacactta gcactcatcg tttacggcgt ggactaccag ggtatctaat cctgtttgct
      241 ccccacgctt tcgagcctca gcgtcagtta cagaccagag agccgccttc gccactggtg
      301 ttcctccata tatctacgca tttcaccgct acacatggaa ttccactctc ctcttctgca
      361 ctcaagtctc ccagtttcca atgaccctcc ccggttgagc cgggggcttt cacatcagac
      421 ttaagaaacc gcctgcgctc gctttacgcc caataaatcc ggacaacgct tgccacctac
      481 gtattaccgc ggctgctggc acgtagttag ccgtggcttt ctggttagat accgtcaagg
      541 gatgaacagt tactctcatc cttgttcttc tctaacaaca gagttttacg atccgaaaac
      601 cttcttcact cacgcggcgt tgctcggtca gactttcgtc cattgccgaa gattccctac
      661 tgctgcctcc cgtaggagtt tgggccgtgt ctcagtccca atgtggccga tcaccctctc
      721 aggtcggcta tgcatcgtgg ccttggtgag ccgttacctc accaactagc taatgcaccg
      781 cgggtccatc catcagcgac acccgaaagc gcctttcaaa tcaaaaccat gcggttttga
      841 ttgttatacg gtatt
//
LOCUS       DACDUU010000204          830 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.213, whole genome shotgun sequence.
ACCESSION   DACDUU010000204 DACDUU010000000
VERSION     DACDUU010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.213"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            7..624
                     /locus_tag="IWL30_002960"
     CDS             7..624
                     /locus_tag="IWL30_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707641.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
BASE COUNT          319 a          103 c          147 g          261 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttctac gttttccttt tcatatatat taaatattac
//
LOCUS       DACDUU010000205          819 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.214, whole genome shotgun sequence.
ACCESSION   DACDUU010000205 DACDUU010000000
VERSION     DACDUU010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.214"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..430
                     /locus_tag="IWL30_002961"
     CDS             <1..430
                     /locus_tag="IWL30_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HAP9707642.1"
                     /translation="TIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLL
                     LDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKE
                     YQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
     gene            complement(560..>819)
                     /locus_tag="IWL30_002962"
     CDS             complement(560..>819)
                     /locus_tag="IWL30_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9707643.1"
                     /translation="KYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLIERLNQEVRR
                     REKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          311 a           80 c          146 g          282 t
ORIGIN      
        1 aactatttat aatgatttaa ctgatataga aaaagattat atagaaagtt ttatggaaag
       61 actaaatgca acaacagttt ttgagggtaa aaagtgttta tgccataatg attttagttg
      121 taatcatcta ttgttagatg gcaataatag attaactgga ataattgatt ttggagattc
      181 tggaattata gatgaatatt gtgattttat atacttactt gaagatagtg aagaagaaat
      241 aggaacaaat tttggagaag atatattaag aatgtatgga aatatagata ttgagaaagc
      301 aaaagaatat caagatatag ttgaagaata ttatcctatt gaaactattg tttatggaat
      361 taaaaatatt aaacaggaat ttatcgaaaa tggtagaaaa gaaatttata aaaggactta
      421 taaagattga ttatataata tatgaaaagc tattataaaa gacattagta ttaaatagtt
      481 taaaaaaatg aaaaataata aaggaagtga gtcaattcca gactcctgtg taaaatgcta
      541 tacaatgttt ttaccatttc tacttatcaa aattgatgta ttttcttgaa gaataaatcc
      601 attcatcatg taggtccata agaacggctc caattaagcg attggctgat gtttgattgg
      661 ggaagatgcg aataatcttt tctcttctgc gtacttcttg attcagtcgt tcaattagat
      721 tggtactctt tagtcgattg tgggaatttc cttgtacggt atattgaaag gcgtcttcga
      781 atccatcatc caatgatgcg caagcttttg aatattttg
//
LOCUS       DACDUU010000206          796 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.215, whole genome shotgun sequence.
ACCESSION   DACDUU010000206 DACDUU010000000
VERSION     DACDUU010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.215"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(313..615)
                     /locus_tag="IWL30_002963"
     CDS             complement(313..615)
                     /locus_tag="IWL30_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707644.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
BASE COUNT          288 a          129 c          107 g          272 t
ORIGIN      
        1 attgtgagat aataataaag aagagtttaa agcgttcccc aaaaaccact tccccataag
       61 tgtgttacgc tttaaactct tttatatttg aagccattaa aaagcatacc atataactgt
      121 aaaaaataat gggaaaaaga cttataattg gagtggtagt taattagtga cttatttttg
      181 attttatagc actgatacta taaaatatag atatcatcat attacacaat cttaatacta
      241 acttaaaaaa tatccctttt ataagtatgg tgataaaatc cgttccgggc tacctttttt
      301 aggtagccta ctttaatctt tatacctttc tggatcaacg aaagtatact tcaaatagtc
      361 ataacgccga tgatcgctcc gtgcgtctgg cacatcagtc acgatatcaa acaaaaaata
      421 cacatctttc ttcattctgg tttttgttgc cggaatttta aagtagttct tattagagta
      481 atagagattg attaataagc tatcttcgat tgctaaaaag aaaacttctg aatcccacac
      541 cttataaaaa tctttgacaa atctattcga aggatcaaat ttaaaccata attgtgtttt
      601 tccttccatc aacataatgt tcacctcaaa aagagtatac gaactaatgt tctttttgta
      661 aagtaggaat atcattgata atatggatgg aacatatata ttaaaaagtg ttctttttga
      721 atgctatttt gaatgccatt ttcattcatt taacgaaaat ctgcgaaaat aaaaaatgct
      781 caaaccctta taaaat
//
LOCUS       DACDUU010000207          766 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.216, whole genome shotgun sequence.
ACCESSION   DACDUU010000207 DACDUU010000000
VERSION     DACDUU010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.216"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            21..>766
                     /locus_tag="IWL30_002964"
     CDS             21..>766
                     /locus_tag="IWL30_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAP9707645.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRG
                     VQEVLLFITDGLKGMKDTIHQIYPKAKYQH"
BASE COUNT          277 a          142 c          133 g          214 t
ORIGIN      
        1 acagaaaaga ggacttccct atgaatgatt ttactacaga aattgtgcaa actctagtca
       61 ctaaaggcga tttaaatgaa ttattccgtt cgcacttaga aaaagcgata aacacactcc
      121 tacggactga attaacggct tttttagatt acgaaaaata tgatcgcact ggttttaatt
      181 caggtaattc gagaaacggt tcttactttc gatcaatcaa aaccgaatat ggtgaattaa
      241 cattggaaat acctagagat cgtaatggtg agtttaaaca acaaacttta ccagcctaca
      301 aaagaacaaa cgatacattg gaaaccacta ttatccattt attcgaaaaa ggtgttacga
      361 tgtctgaaat tgctgatttg atcgaaaaaa tgtacggtca tcactatact ccacaaacca
      421 tgtccaacat gactaaagtt ctgactgaag aagtaaatgc ctttaaatcc agagccttaa
      481 atgataagta tgtcgctatt tttatggacg ctacttacat tccactaaaa cgtcaaaccg
      541 tatccaaaga agcgatttat attgccattg gtatacgaga agacggcact aaagaagtac
      601 tgagttatgc gattgctcca actgaatcaa catacgtttg gaatgagctg ctacaggata
      661 ttaactccag aggagttcaa gaagtcttgc tttttattac ggacggctta aaaggcatga
      721 aagatactat ccatcaaatt tatcctaaag caaaatatca gcattg
//
LOCUS       DACDUU010000208          765 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.217, whole genome shotgun sequence.
ACCESSION   DACDUU010000208 DACDUU010000000
VERSION     DACDUU010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.217"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..276)
                     /locus_tag="IWL30_002965"
     CDS             complement(<1..276)
                     /locus_tag="IWL30_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9707646.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDV"
BASE COUNT          259 a          128 c          128 g          250 t
ORIGIN      
        1 gacgtcaggc gtccaaccaa gagtgacttt ctctttaatg taatctactt catgagcagg
       61 taattgaatg acttttctac cacatttttt cttattttct ttatactggt gccaataatc
      121 aagagcagtc ttgccttgct tgaacttatt gactacatta taaatagttt gtatagcacg
      181 tcccattcgg ttagcgattt caaccggctt attatgttga agataatatg attctatcat
      241 tgtcagttcg tctatggtaa gatgtttgta ggtcatttat ggttacactc ctttgttttc
      301 tttcgtcgga aatacaattt gagtgtacca taaatgcctt tttatttttc taacttaatt
      361 ttacaattcg cgatatatta aacaacgttg tcgcgcttca ttgaaacatt ttatgaaaat
      421 tccaaaaaag agttgacaca tgtttttaga cgtgataaga taactaagtc gttaaaaaga
      481 accacttgcg aagcttgatt ctatcgaaaa aaaacttcaa aaaagttctt gacaaataac
      541 aaacgataag ttatgataaa tgagttgctg aaagatttcg aaaaacgaaa tcgaaaaaaa
      601 cttttaaaaa agagttgaca atcgcttgtc tctctgatat gatataaaag ttgctacggc
      661 agccaaaaac gaatcgggaa acactcccga tgaagtagat ctttgaaaac tgaacaaagt
      721 aagacaaacc aaatgtgtag ggcgtcttga ttcaattcaa gacaa
//
LOCUS       DACDUU010000209          744 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.218, whole genome shotgun sequence.
ACCESSION   DACDUU010000209 DACDUU010000000
VERSION     DACDUU010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.218"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(91..>744)
                     /locus_tag="IWL30_002966"
     CDS             complement(91..>744)
                     /locus_tag="IWL30_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HAP9707647.1"
                     /translation="GRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPL
                     EAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDV
                     KLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQF
                     ETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          203 a          174 c          141 g          226 t
ORIGIN      
        1 ttgtgaatgc tgcggtgtta tggctacgat tgcccttgac aatgagcatc ctcggatctg
       61 attttctccg gcagaagcac aatcattcaa ttacgctgcc tctccagagg tatcagatcc
      121 gcgctcatta tcaaggattc gcttcgccaa tctctgttgt cgaatttgga tcggtgtttc
      181 atacgccaac gtgccatgta agcgtagata gttccaccaa tgcacataat caaacaattc
      241 tagacgtagc tgtgccagtg tctcaaattg gtattgatgc acgaattcta ctttgacaga
      301 tttataggtt gattccacaa cggcattatc atacggacaa cccttcctac tcaatgaacg
      361 agtaattcca aaaccattca gaatctcatg aatggtttgg ttatcaaatt cctttccccg
      421 gtctgtatga aaaagcttga cgtctgttaa agaatacggt atccgtccaa aggcttcttt
      481 taccaatgag gcatctttct tctcaccaca ggaataacca ataatttctc gattaaacaa
      541 atcaagtatt aagcagatat aatgccactt tttccccacg cgaacataag taagatccgt
      601 aacgatggct tccaatggct gttcttgtgt aaaggtccga tctaatacat tcgctgtttt
      661 cgcttcatta caagctgttc gttgcccttt aaaatgagcg atcgtatagg tagatgtcaa
      721 tccgcggcgt ttcatgattc gacc
//
LOCUS       DACDUU010000210          714 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.219, whole genome shotgun sequence.
ACCESSION   DACDUU010000210 DACDUU010000000
VERSION     DACDUU010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.219"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
BASE COUNT          260 a          121 c           90 g          243 t
ORIGIN      
        1 taattcgctt gtggcgaaac ccgacctgga gtttgtctct gtattttgaa ccctaaaaat
       61 taatcaaaca gactgatatt ctcataatca gcgcgaaata agccattaag tacagttttc
      121 ctacctattt ttgataaccc gctggttagt taatattcct tttccgagag agataaatga
      181 ttggttaaat ctatatacat ctcaaccttt aaaaatgttt tcgaaatact tcatcagtac
      241 atgtcataca aattaccagt gcattctcaa attctagttc tttaattgat ttcagttgag
      301 gagcagttac ttttggcgga aatttccatt tttgtccaaa cctcttcccc cgatgaaatt
      361 tctttgttgc aattattgca atgaaaaatt tttgtttcaa aaatatacat aattacttcc
      421 tccaataaaa actatctacc tctcaaatta tttttctcta ataagaaaaa cagaaaaggt
      481 aataagatac aacagtgatg attcctaata aagaaaagac tacaataata tttttctaac
      541 ttatcgcctt caaagaaata gtttcttatt tttcactaaa ataaaaaatt tattgcaaaa
      601 aaaatagaag tgacaaaatt atcaatcatt tcaaggtaat ataactacct gagtttatta
      661 tcgattaaaa tgctaactac atttgcaaga gaagcgagag ttaaaggcaa atat
//
LOCUS       DACDUU010000211          703 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.220, whole genome shotgun sequence.
ACCESSION   DACDUU010000211 DACDUU010000000
VERSION     DACDUU010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.220"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            330..>703
                     /locus_tag="IWL30_002967"
     CDS             330..>703
                     /locus_tag="IWL30_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HAP9707648.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEV"
BASE COUNT          267 a           78 c          113 g          245 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggt
//
LOCUS       DACDUU010000212          675 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.221, whole genome shotgun sequence.
ACCESSION   DACDUU010000212 DACDUU010000000
VERSION     DACDUU010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.221"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            110..322
                     /locus_tag="IWL30_002968"
     CDS             110..322
                     /locus_tag="IWL30_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707649.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            484..>675
                     /locus_tag="IWL30_002969"
     CDS             484..>675
                     /locus_tag="IWL30_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707650.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDI"
BASE COUNT          237 a          100 c          121 g          217 t
ORIGIN      
        1 gtgagtatct ccattcataa aataatcaac atatttaaaa aatctaatta gcttttttag
       61 tcaatctaaa gttctttatt ctatgatata atgttactgt agaaagtttg tggcggtttg
      121 gcaaatccaa ttgaaggagc gtggtgccta tgggcttatt agcttatcgg acgtactctg
      181 tagaaaggaa atgcctgatt tgttctcatt ggatcaaatc attgctatta tcggagcagc
      241 agcagtagtt attactgccg tagcgaaagc tagcgattcg attttagctt ggttaaagtt
      301 caaaaaagaa caaaaaaaat agagccgtct caacttttgg cgaggtagac gagctctaaa
      361 gctttattta catgccatcc gctttgaacg gactacactg ggcatgtgac agcatgccct
      421 ttcatttata ttatatctca ttcttttata taatgaaagt aaataaatca tggaggataa
      481 aatatggata aatttaatag attaacatta ataaatcagt ttgaaatttt aaaagcttta
      541 aatcctaacg aagaaaaata ctatgctgaa aaaatagaaa tactaacaga aggttacgaa
      601 tatcactata gtgaaatatt tgaaaatatt tctgatccat tgccagaaga agattcgcgg
      661 tttgttctag acata
//
LOCUS       DACDUU010000213          656 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.222, whole genome shotgun sequence.
ACCESSION   DACDUU010000213 DACDUU010000000
VERSION     DACDUU010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.222"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="IWL30_002970"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="IWL30_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP9707651.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          198 a          143 c          111 g          204 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaa
//
LOCUS       DACDUU010000214          653 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.223, whole genome shotgun sequence.
ACCESSION   DACDUU010000214 DACDUU010000000
VERSION     DACDUU010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.223"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..303)
                     /locus_tag="IWL30_002971"
     CDS             complement(<1..303)
                     /locus_tag="IWL30_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
                     /protein_id="HAP9707652.1"
                     /translation="MKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTN
                     KKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYST"
BASE COUNT          215 a          103 c           69 g          266 t
ORIGIN      
        1 agtagaataa atttctggtg ttaaaaaagt tcctttaatt tctttataac ctagtataga
       61 taattcatca ctaatatacg aatattcaat attaggaatt tttacattag tttctaaatt
      121 tgtatttaaa aaattatata ttgctttttc ttttgcataa ccttttttct tattagtact
      181 aaattttgtt ttaaaaatgt attcattatt aactaaatat gccacactat cataaccact
      241 accgattatt tcaatactat ctactttgaa attatcaaag taatgctcaa ttaaatattt
      301 cattgcctta acatttgtgg cattatcatc atatctatat tccattaaat aacaatcttc
      361 ttttttgccc tcgtgtaatt catgttctgg caaatcttca ataattctaa aaccagattt
      421 ttggtatgcc cttattgctc ttggattatt tttatgaggg tctaaaataa ctgcattagc
      481 atttctttct tttttcaaaa attcaaaaat caatttaata tatcttgtac ggttctgttg
      541 caaagtttta aataaagaat aaaatccctt acggtatcta tgatttaagc tgggattccc
      601 aataatacct tgatttcagt acagaccgaa aacccgaaga gagtgccttc ttt
//
LOCUS       DACDUU010000215          602 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.224, whole genome shotgun sequence.
ACCESSION   DACDUU010000215 DACDUU010000000
VERSION     DACDUU010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.224"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
BASE COUNT          222 a           75 c           74 g          231 t
ORIGIN      
        1 attttgctcc acttttagat aatttttagg tctatttaac tcatcagaca ttctatgctc
       61 atttttctcc aaatttgttt caactctttc gtacttaatt ttgcgtattt gaaaattctt
      121 cttttagagt acttttatta cgatactagt atgatatata tctatagcaa acagttaaaa
      181 tcttcctaaa ataagcgaat agcattccta aaacctataa aatttttaga tgaagcgaca
      241 agtggacaat taataaacta cttgaacatg aaattatgga tcacctttca aatttagaaa
      301 atggaattct tatacttatt gcgcaacatt tattgattgc aaaattatgt gacaaaaaaa
      361 actagtagaa gaaagaataa tggcttgata ataaagtact acctattaaa ggtagtactt
      421 tatttaattt agtataaatt aacatactac tatattttag gtttcatatt atataaatta
      481 ataatatact accatttgtt ggtagtataa tttaaaggta ataagttatt cttttattac
      541 tatgtttggg ttttatagca aagaaagttt aataagagtt tggacctagc aaacaatatt
      601 aa
//
LOCUS       DACDUU010000216          536 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.225, whole genome shotgun sequence.
ACCESSION   DACDUU010000216 DACDUU010000000
VERSION     DACDUU010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.225"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>536
                     /locus_tag="IWL30_002972"
     CDS             <1..>536
                     /locus_tag="IWL30_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9707653.1"
                     /translation="KIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDYSTSQEIL
                     NNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEESLESVSGI
                     KYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQFIFELIFI
                     SIPSIISSLFLGNVLLKV"
BASE COUNT          210 a           49 c           88 g          189 t
ORIGIN      
        1 gaaaaataaa aagtcataaa tttaaaatta tagggatctt ttctggtaaa aaacaggaaa
       61 catatacagg attatcttct gattttagtg aaaatatggt gtttgtagat tattcaacaa
      121 gccaagagat attaaataat tcagaaaata atgaaattgc aaataaaatt ttaatgtatt
      181 ctggtagtgc agaatctaca gacttagcct taaacaaatt gaaagagctt aaaattgatg
      241 agtcaaagta ttttgttgaa aaagattcta atgcgtttga agagtcttta gagtcagtga
      301 gtggaataaa atatataata aaagtaatga cttattctat tatgttaggt gggatggttg
      361 ttctttcatt aatcttgatt ctatggttaa gggaaagaat ttatgaaata ggtatattct
      421 tatctattgg aacatctaag atacaaatta taatgcaatt tatattcgag ttaatattca
      481 tatcgatacc aagtataata tcttccttat ttttaggaaa tgtattacta aaagta
//
LOCUS       DACDUU010000217          533 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.226, whole genome shotgun sequence.
ACCESSION   DACDUU010000217 DACDUU010000000
VERSION     DACDUU010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.226"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..285
                     /locus_tag="IWL30_002973"
     CDS             <1..285
                     /locus_tag="IWL30_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9707654.1"
                     /translation="EVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHM
                     LQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT          161 a           92 c          120 g          160 t
ORIGIN      
        1 gaggttgcag aagatgatcc ttattgggaa atcgaagcct cctatattgc tcaatgtgcc
       61 tacaatacaa ccttgatgtt ttctccagat gtgatcattt ttggcggtgg tgtgatgaaa
      121 caacgccata tgctgcaaaa cgtccacgac gcatttgcaa aattggtcaa tgggtatgtc
      181 gaaacacctg agttaagcaa atatatcgtg accccagcat tagaagacaa cgctggaacg
      241 ctcggttgtt tggcattggc tcgagaagcg gtcttacatt cataaacgaa aaaaggctct
      301 tcgacattta gtgttggtag agaaagatac aactgaaaat ttagtcataa ccagttaggc
      361 agagtgctta actggttttt gttttacttg gaagatcaag ccttgaatga gataaattct
      421 tgctcgaaag ttatggaaat ttcgatagcc gtaggccact tgtattgtgc aatagttttt
      481 tacaaaaaaa agggggtgcg gatatttttt tggacctttt taacaaatat atc
//
LOCUS       DACDUU010000218          528 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.227, whole genome shotgun sequence.
ACCESSION   DACDUU010000218 DACDUU010000000
VERSION     DACDUU010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.227"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(5..90)
                     /locus_tag="IWL30_002974"
     tRNA            complement(5..90)
                     /locus_tag="IWL30_002974"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="IWL30_002975"
     tRNA            complement(107..178)
                     /locus_tag="IWL30_002975"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="IWL30_002976"
     tRNA            complement(190..262)
                     /locus_tag="IWL30_002976"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="IWL30_002977"
     tRNA            complement(280..361)
                     /locus_tag="IWL30_002977"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="IWL30_002978"
     tRNA            complement(369..441)
                     /locus_tag="IWL30_002978"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="IWL30_002979"
     tRNA            complement(445..517)
                     /locus_tag="IWL30_002979"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DACDUU010000219          483 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.228, whole genome shotgun sequence.
ACCESSION   DACDUU010000219 DACDUU010000000
VERSION     DACDUU010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.228"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            175..>483
                     /locus_tag="IWL30_002980"
     CDS             175..>483
                     /locus_tag="IWL30_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707655.1"
                     /translation="MSIDDCLEEQIRLFLKFLKVKVQNNYFTCYSYAHPVLNSLPFNS
                     LVQGLITNIVLLVCTPNEIFVKKIGSGISSVRLKEKEYTQKIIHIPKPEIKRFEIKDY
                     S"
BASE COUNT          166 a           48 c           81 g          188 t
ORIGIN      
        1 acattcggta aaactacttt ttctagtttt aattttcagt ttctattaaa aaataatttt
       61 tatggtttat tgaaataagg ttgcgaagga agatgtagag ttatttgcac tgttgtgttg
      121 gtatatctct taatgctgtg tctttttggg gagattaatt agtggagtga ttatatgagt
      181 atagatgatt gtttagaaga acaaataaga ttatttttaa agtttttaaa agttaaagtt
      241 caaaataact acttcacatg ttatagttat gcacaccctg ttttaaatag tcttcctttt
      301 aattctttag tgcaaggatt gattacaaat attgttttat tagtctgtac accaaatgaa
      361 atatttgtaa aaaagatagg cagtggtatt tcttcagtaa gattaaaaga gaaagaatat
      421 actcaaaaga ttattcatat tcctaagcct gaaattaagc gttttgagat aaaagactat
      481 tct
//
LOCUS       DACDUU010000220          469 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.229, whole genome shotgun sequence.
ACCESSION   DACDUU010000220 DACDUU010000000
VERSION     DACDUU010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.229"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            8..322
                     /locus_tag="IWL30_002981"
     CDS             8..322
                     /locus_tag="IWL30_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707656.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          152 a           56 c           71 g          190 t
ORIGIN      
        1 atagcttttg gatttttaca gtaattttat cttaataata gcaatattat tacttctaaa
       61 tatttggttt tttgataaga gtagaaatgc agggataggt tttagaacca aacgaagtac
      121 gtcttctgaa aaaaagtggg tatattcaca gactattttt tacggagggg ttatttcaat
      181 cagtttactt tcttcaacac tttattcttt taatgttata gacgtttcca tgtcaaattt
      241 tatatctatt attggaatcc ttatttctgc aattattaca caactacttc ttgtgtttga
      301 agagaagtca aaaaataatt aaagcaatta tgccttttag tacaggaatt attacttctt
      361 acaaatgtat agcgaatgtg aagcctgctg ttttaattgt tctagaaaaa cttttgagtg
      421 aacaattatt ttatgttagg atgaattaat ttctctagag tttataatt
//
LOCUS       DACDUU010000221          460 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.230, whole genome shotgun sequence.
ACCESSION   DACDUU010000221 DACDUU010000000
VERSION     DACDUU010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.230"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(20..91)
                     /locus_tag="IWL30_002982"
     tRNA            complement(20..91)
                     /locus_tag="IWL30_002982"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="IWL30_002983"
     tRNA            complement(113..200)
                     /locus_tag="IWL30_002983"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="IWL30_002984"
     tRNA            complement(225..298)
                     /locus_tag="IWL30_002984"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="IWL30_002985"
     tRNA            complement(312..382)
                     /locus_tag="IWL30_002985"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="IWL30_002986"
     tRNA            complement(384..459)
                     /locus_tag="IWL30_002986"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
BASE COUNT          132 a          128 c          108 g           92 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca
//
LOCUS       DACDUU010000222          443 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.231, whole genome shotgun sequence.
ACCESSION   DACDUU010000222 DACDUU010000000
VERSION     DACDUU010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.231"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            22..>443
                     /locus_tag="IWL30_002987"
     CDS             22..>443
                     /locus_tag="IWL30_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9707657.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIEKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRFKTGEFNVLHLPMQGKRKPNGYKEKRGKQA"
BASE COUNT          160 a           84 c           91 g          108 t
ORIGIN      
        1 atacaaggag agtgaacata aatgacttat acccatctta caccaaacga gcttgtaatg
       61 atagaagcat attttcatca agaaactccg gttgctatcg ttgcgaagca gcttaaacgt
      121 ggacgccaaa caatttacaa tgtctataac tttctcaaat gtggtggaac agcacttgaa
      181 tactttgaac aatacaaaga aaataagcga cgttgtggga gaaccgaaat catttttcct
      241 gctgaggaaa aagaatacat tgaaaaaaga tcaactgaag gttggacccc agacgtcatt
      301 attggccgtg cagagcgaac cttctcttgt tcagtaagta ccctctatcg ccggtttaag
      361 acgggagaat tcaatgtttt acatttaccg atgcaaggaa aacgaaaacc aaatggttat
      421 aaggaaaaac gtggaaaaca ggc
//
LOCUS       DACDUU010000223          404 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.232, whole genome shotgun sequence.
ACCESSION   DACDUU010000223 DACDUU010000000
VERSION     DACDUU010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.232"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>404
                     /locus_tag="IWL30_002988"
     CDS             <1..>404
                     /locus_tag="IWL30_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707658.1"
                     /translation="PEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDK
                     SDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVV
                     VTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          166 a           60 c           92 g           86 t
ORIGIN      
        1 ccagaagata aacttccagt gttgaaagca aaagatggtt atacagatgc gaaatggcca
       61 gaggaagcaa ctcaaccaat tacagcagat gatacagaat ttgtatcaag tgcaacaaaa
      121 ctggatgata aatctgacgc tgacaaatat aatcctgaag gtcaaaaagt gactacagaa
      181 ttgaataagg aaccagacgc atttgaggga attaagaaca aagaagattt accaaaagat
      241 actaagtata cttggaaaga aaaagtagat gttagcgcag ctggaaataa aaaagggact
      301 gttgtagtga catatccaga tggatcatct gatgaagttg aagtagatgt tacagtgaca
      361 gacaatcgtt ctgacgccga taaatatgag ccaacagtag aagg
//
LOCUS       DACDUU010000224          391 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.233, whole genome shotgun sequence.
ACCESSION   DACDUU010000224 DACDUU010000000
VERSION     DACDUU010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.233"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>391
                     /locus_tag="IWL30_002989"
     CDS             <1..>391
                     /locus_tag="IWL30_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HAP9707659.1"
                     /translation="KKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWLRKKRDT
                     QAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLNNLIEQ
                     DHRPVKRRNKFYRSLRTASTTIKGMEA"
BASE COUNT          141 a           72 c           75 g          103 t
ORIGIN      
        1 ataaaaaatc cttttattca tggaaaatgg atgaaacgta catcaaaatt aaaggaaaat
       61 ggcattattt gtatcgagcc atcgatgcag atggtttaac cttggatatt tggttacgta
      121 aaaaacggga cacacaagca gcctatgctt ttcttaagcg gttagtgaag cagtttgatg
      181 aaccgaaggt tgtagtcaca gataaagccc cctctattac aagtgccttt aagaaactaa
      241 aagaatacgg cttttatcaa gggacagaac atcgtaccat taaatacctg aataatttga
      301 ttgaacaaga ccatcgtcca gtaaagagac gcaataaatt ctatcgaagt ttacgcactg
      361 cctctaccac gattaaaggc atggaagcca t
//
LOCUS       DACDUU010000225          385 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.234, whole genome shotgun sequence.
ACCESSION   DACDUU010000225 DACDUU010000000
VERSION     DACDUU010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.234"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..>385)
                     /locus_tag="IWL30_002990"
     CDS             complement(<1..>385)
                     /locus_tag="IWL30_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HAP9707660.1"
                     /translation="NRNNTTAFKIFRAIINRCLRMIKLPPKIRHHKFDLYHGSQWWAL
                     SKECLTELIQMYEQNQQDYLNFKIGMFAPDEKFFHTLFFNSSFKNKNVIGGPDLPLEL
                     KNIEETTLQTSKLANIHIIDPSMNKW"
BASE COUNT          126 a           53 c           59 g          147 t
ORIGIN      
        1 ccatttgttc atggaaggat caataatatg aatatttgct aatttcgaag tttgtaaagt
       61 agtttcttca atatttttca attcaagtgg taaatctggt cctccaataa catttttatt
      121 tttgaacgag gaattaaaaa aaagtgtatg aaaaaatttt tcatctgggg caaacatacc
      181 tattttaaaa ttcaggtagt cttgttgatt ttgttcatac atttgtatta attctgttag
      241 gcattctttc gacaaagccc accattgaga accatgatac aaatcaaatt tatggtgtct
      301 aatttttggg ggaagtttaa tcatcctcag acaacgatta attatagctc taaaaatttt
      361 aaatgcagtt gtgttattcc tatta
//
LOCUS       DACDUU010000226          381 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.235, whole genome shotgun sequence.
ACCESSION   DACDUU010000226 DACDUU010000000
VERSION     DACDUU010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.235"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(21..94)
                     /locus_tag="IWL30_002991"
     tRNA            complement(21..94)
                     /locus_tag="IWL30_002991"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ile,seq:cat)
     gene            complement(108..181)
                     /locus_tag="IWL30_002992"
     tRNA            complement(108..181)
                     /locus_tag="IWL30_002992"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Met,seq:cat)
     gene            complement(209..281)
                     /locus_tag="IWL30_002993"
     tRNA            complement(209..281)
                     /locus_tag="IWL30_002993"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Ala,seq:tgc)
     gene            complement(301..374)
                     /locus_tag="IWL30_002994"
     tRNA            complement(301..374)
                     /locus_tag="IWL30_002994"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(338..340),aa:Pro,seq:tgg)
BASE COUNT          103 a          108 c           90 g           80 t
ORIGIN      
        1 tgaaacttac aacgccacaa tggaccttgt aggactcgaa cctacgaccg gacggttatg
       61 agccgtctgc tctaaccaac tgagctaaag gtccaggctc ttaaaaatcg cggcggaggg
      121 gatcgaaccc ccgacctccc gggtatgaac cggacgctct agccagctga gctacaccgc
      181 gaaaaagata ttcattattt aaatgaaatg gagcctagcg ggatcgaacc gctgacctcc
      241 tgcgtgcaaa gcaggcgctc tcccagctga gctaaggccc cgtgtttaaa tttataatta
      301 tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc tctaccaagc
      361 tgagctactt cccgtttaat t
//
LOCUS       DACDUU010000227          375 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.236, whole genome shotgun sequence.
ACCESSION   DACDUU010000227 DACDUU010000000
VERSION     DACDUU010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.236"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..132)
                     /locus_tag="IWL30_002995"
     CDS             complement(<1..132)
                     /locus_tag="IWL30_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP9707661.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          132 a           69 c           76 g           98 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaat
//
LOCUS       DACDUU010000228          341 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.238, whole genome shotgun sequence.
ACCESSION   DACDUU010000228 DACDUU010000000
VERSION     DACDUU010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.238"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
BASE COUNT          159 a           43 c           28 g          111 t
ORIGIN      
        1 agaaaacaat agattatata ctactaaaaa atacaataag tactaccaat gtcagatata
       61 tataataatt atgtactacc attaaaatta acttatttaa taagatacta ctgtgttaat
      121 ctgaaaaaaa ttactttaaa gtagttacat actacctgtt aatttagata ctaccattaa
      181 tacttaaatt agatactacc tatatttata ttaagtacta ccgttctaag aataagataa
      241 taaaaaaata ctaccaaatt gcaattaaat actacttaaa atgtaaaaaa taagaactac
      301 ccaaattagg tagttcttaa taagagaata tcagataaat t
//
LOCUS       DACDUU010000229          332 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.239, whole genome shotgun sequence.
ACCESSION   DACDUU010000229 DACDUU010000000
VERSION     DACDUU010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.239"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
BASE COUNT          119 a           48 c           58 g          107 t
ORIGIN      
        1 tggttgttgc cacataaagc cataaacaat caaccagtag aatgcgtggc aacaaacctg
       61 ttatcgcata tcttggagtg tgactattta tgagtgatag tgaagatatg cgtctccata
      121 tatgtatggt tgagacagat aaacatgtat gaaaaacgtt gttaaatcaa cgttttttta
      181 ttttggtcaa ggagagaaaa gtagagaaat acaaaatttt ttgcattgat tttgcattca
      241 aaaaaacatt caaacattta gaaatcccca atacgtattc aggaaaatct attttaaggt
      301 ttacaaaaag aacttttgtt cgtacactct tt
//
LOCUS       DACDUU010000230          327 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.240, whole genome shotgun sequence.
ACCESSION   DACDUU010000230 DACDUU010000000
VERSION     DACDUU010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.240"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>327
                     /locus_tag="IWL30_002996"
     CDS             <1..>327
                     /locus_tag="IWL30_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HAP9707662.1"
                     /translation="YVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANA
                     NKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKG"
BASE COUNT          122 a           51 c           74 g           80 t
ORIGIN      
        1 ttatgttcgc cgatttagag aaacgacggt gtttgaggat atttttgcct atattttaga
       61 acaagcagtt aaagcaggtt ttgtaacaga ggataatttg tatctggatt ctacccacat
      121 caaagctaac gccaataaac ataagtttac gaaagaaatg acccatgatg aagcgaaagc
      181 ctatcaggat gaattagaag atgagatcaa tcgtcaacga atagaggctg gaaaacgccc
      241 ttttactttg gacttagaaa aggaagtgaa gctgaaagaa agaaaaataa gtaaagctga
      301 tccggaaagc ggctactacg tcaaagg
//
LOCUS       DACDUU010000231          325 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.241, whole genome shotgun sequence.
ACCESSION   DACDUU010000231 DACDUU010000000
VERSION     DACDUU010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.241"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..315
                     /locus_tag="IWL30_002997"
     CDS             <1..315
                     /locus_tag="IWL30_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HAP9707663.1"
                     /translation="IKIVLSASIPILIDQASEHRSLLLIVSIASAIITIIQSGMSAFN
                     YQDKVQTSTELLMKIEKEKLLYITKTSPYNKTDEENFHLIVTTLQTELNDIISDFNQV
                     NN"
BASE COUNT          131 a           47 c           39 g          108 t
ORIGIN      
        1 ataaaaatag tcttatcagc tagtatccca atattaattg atcaggcttc cgaacacagg
       61 tctttactat tgattgtttc aattgcttca gcaataatta caattataca aagtgggatg
      121 tctgcattta attatcaaga taaagtacag acttcgacag agttattaat gaaaattgaa
      181 aaagaaaagt tgttatatat aacgaaaaca tccccatata ataaaacaga tgaagaaaat
      241 ttccatttaa tcgttacaac ccttcaaact gaattaaatg atattatctc tgattttaat
      301 caagttaata actaaaaatt tattt
//
LOCUS       DACDUU010000232          324 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.242, whole genome shotgun sequence.
ACCESSION   DACDUU010000232 DACDUU010000000
VERSION     DACDUU010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.242"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            53..>324
                     /locus_tag="IWL30_002998"
     CDS             53..>324
                     /locus_tag="IWL30_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9707664.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDT"
BASE COUNT           95 a           57 c           62 g          110 t
ORIGIN      
        1 tttatggatg atgtctacag tcttttcttt tataatagat ttatgaaaac gaatgcaagg
       61 agtgatagct atgatgtcta aaaacaatac gaatggtaga aatcaatttg cgatgcttac
      121 gattgatgat ctagttcctc aagatcatct cgttcgaaag attgatgccg cgcttgattt
      181 tgaatttatt tatccaatcg tcgaagccac ttactccgat ttaggtcgcc caagtatcga
      241 tccagtgatt ctgatcaaat tggtgtttat tcagtattta ttcggtatcc gttccatgcg
      301 acaaaccatt aaggaagtcg atac
//
LOCUS       DACDUU010000233          321 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.denovo.243, whole genome shotgun sequence.
ACCESSION   DACDUU010000233 DACDUU010000000
VERSION     DACDUU010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.denovo.243"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>321
                     /locus_tag="IWL30_002999"
     CDS             <1..>321
                     /locus_tag="IWL30_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9707665.1"
                     /translation="TQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTK
                     TEHRTVKYLNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFG
                     FSVST"
BASE COUNT          107 a           69 c           63 g           82 t
ORIGIN      
        1 acgcaagcag cctatgcttt cttaaaacga ctccataaac agtttggtga gccgaaagca
       61 attgtgaccg ataaagcacc ttctcttggc tccgccttta gaaagttaca gagtgtgggt
      121 ttatatacta agacagagca ccgaactgtg aagtatctta acaatttaat agaacaagac
      181 catcgaccta ttaaacgacg aaataaattt tatcaaagtc tccgtacagc ctcttccacg
      241 attaagggca tggagaccat tcgaggaata tataaaaaga accgaagaaa tggaacgctc
      301 ttcggctttt cggtgtctac t
//
LOCUS       DACDUU010000234         3517 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.guided.137, whole genome shotgun sequence.
ACCESSION   DACDUU010000234 DACDUU010000000
VERSION     DACDUU010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.guided.137"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..203
                     /locus_tag="IWL30_003000"
     CDS             <1..203
                     /locus_tag="IWL30_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP9707666.1"
                     /translation="MQFMLVARDHGYETNPIGGFEADQLAEAFGLDKDRYVPVIILSI
                     GKAVEEGYESVRLAPEKITMFE"
     gene            557..2035
                     /gene="msr"
                     /locus_tag="IWL30_003001"
     CDS             557..2035
                     /gene="msr"
                     /locus_tag="IWL30_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP9707667.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            2082..2528
                     /locus_tag="IWL30_003002"
     CDS             2082..2528
                     /locus_tag="IWL30_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP9707668.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2727..3242)
                     /locus_tag="IWL30_003003"
     CDS             complement(2727..3242)
                     /locus_tag="IWL30_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9707669.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            3359..>3517
                     /locus_tag="IWL30_003004"
     CDS             3359..>3517
                     /locus_tag="IWL30_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9707670.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLN"
BASE COUNT         1130 a          583 c          737 g         1067 t
ORIGIN      
        1 ctatgcaatt catgttagtt gcgcgtgatc atggctatga aacaaatcca attggtggat
       61 tcgaagcaga tcagttggca gaggcatttg gtttggataa agaccgttat gttccagtta
      121 ttattttatc aataggaaaa gcggtagaag aaggttacga gtctgttcgt ttagcaccag
      181 aaaagatcac tatgttcgaa taaaaggttt tcagttacta ttcagtagct attgcttttt
      241 ttgaatcgtg tgtggtaacc taataaaaaa ggaggtcgga aaaatgactg catcgatgaa
      301 attacgtttc gaacttttga ataacaacta aataaattca aaagacatgt ataaacaaca
      361 atttcatgga tggagtatgt ccattttttc gaacgacagt agaataggtg aaagctatga
      421 cagatttggt gatttagtca tagttatttc gttcagagta ggaaaagatg tgatgcagtt
      481 tttttgcaaa cagatttttc tgctcttttt tagtatcgat aattgttgtt ttacatgaaa
      541 taggagtgta aaacatatgg aaaatttagc agtaaatata acaaatctac aagtcagctt
      601 tggcaaccag ctagaactat ctattgattc tcttcgtgtc tatcagcaag atcgtatagg
      661 gatcatagga gaaaatggtg tcggtaaatc gactttgctc aaactaatag ccggtgaact
      721 ttttcccgat catgggaaaa tccaaacaga gatcaccttc aactacctgc ctcaattaac
      781 ctatctcgct gaagcaaagg acctaaattt ggaattagcc agtcatttcc agttaagact
      841 ggaagaaact tcagagcgga aatggagcgg aggggaagaa cgaaagatcg agttgatacg
      901 tcttctttct tcttatgaac aagggatgct tctagacgag ccaacaaccc atctagatag
      961 aaaaagtatt gatcgactga ttgaagagct tcgttattat tatggtacgc tggtttttgt
     1021 tagtcatgat cgctattttc tagatgagct agcatcgaaa atctgggaag taaaagacgg
     1081 agaaatccga gagttttcgg ggaattatag tgcctatctc actcaaaagg aattggagaa
     1141 aaagactcag ctacgagaag cagagtcgat catgaaagag aaaaaacgat tggaaaaatc
     1201 gatccaagaa aagaaaaaac aagcggaaaa gttggaaaaa gtgtccagta aaaagaaaaa
     1261 gcaacaaatc agactggatc ggttgtcttc ctctaaacaa aaagacagtg tacaaaaagc
     1321 catccaaaag aatgcgaaaa cattagagag aagactccaa aaaataggag aaacaaccaa
     1381 gccgcagcag atgaaacaaa tccgttttcc agtaccaaaa tctcttgaac tccacagccg
     1441 ctatcccatc atgggacaaa atgtccaatt ggaaagaagt gggagaacat tactggtaaa
     1501 tggtgatttt cagttttctt taggtaaaaa aatcgcgatt gtcggtgaaa atggttcagg
     1561 taagacaacc ttattagaac atatccgcaa acaaggagaa ggaatccttc tctctccgaa
     1621 agtaagcttt caagtatatc agcaaaaggg ttatcaaatg acatctgaag aatccatcat
     1681 tcgttttgtc atgagacaaa cagagttttc agaatcgctt gtccgtagtt tgctgaatca
     1741 cttagggttt gctcaggaaa ctttgacgaa accgttatgt acattaagtg ggggagaagc
     1801 gacccgtctg acgattgctt tgctttttac taagccaagt aatgtgttgc tgttagatga
     1861 accgactaat tttattgata tggcaacgat cgaagcttta gagaagctaa tgcaaatata
     1921 tccgggaacg attttgttta cttctcatga ttcttacttt gtcgagcgta cggctgatga
     1981 agtttatgaa ataaaagggc agaaaataaa aaaagtactt acgagaaatt tttaaatgtg
     2041 ctacgatgat tttcgtgaca aatttaaagg agtggtacag attgcctaca aataaaaaag
     2101 aaggaataat ttttacaaca atgatgtgtt ttttgatggt cttggggatg agcgcgtata
     2161 atttatggct gcatcaagat cttcatttat caagtttgtt gattggtcta gtgcctggat
     2221 tcgtggtggc tttcattttg gatgtcttca tcatcggtgt gctggcgaaa aaaattgcct
     2281 ttaaactacc agtaaatcca gaaagcaagg tccaaatgat cttaactatt tcatgcttga
     2341 tggttttagg aatggtgaca tgtatgtctt tattcggtgt gatcatggaa caaggaatac
     2401 ctgatcaatt attctctgct tatatgaatg cttggaaaat gaatgtgatt gccgctttac
     2461 cgttgcagtt gatcatcgtt ggcccgcttt caagaagtgt gctaaaagca attcaaagta
     2521 atgaatgatg gaacactgtt atttgaaaaa aggccttggc atgctttttg tcatagtctc
     2581 ttgaatgcag ttctagaaaa atagtgatcc gaacgatagg ttttggcctg tcgttcggat
     2641 cgctttttct tttccaactg tattgctctt ttattgaaga taatttgctc ttcaatagat
     2701 atttctagaa aggttctgtt tatcccttac tcaaaaaact gctgcatttg ttctcttact
     2761 tgctgacacc attggagttc ctgctgacaa agcataattc tgcgctggat cacaacataa
     2821 cgtccaaatt cttgtttctt gtctagcgag aggtcattca attttttttg atttttttgc
     2881 atgattttgt tcagctgttg ttctctagtg aatagcagtc cagtagctgt tgttttatcg
     2941 agcgtcgaaa aagcatagat ttttgctaga aattcatctt tttgcgtagg agcgggagtt
     3001 tcctctttga tccattcctc tacaagagca cgtccttcat cagtcaagtg gtagatcttt
     3061 ttttgagagt gctcgtcttt gtccaaaacg gtcacataac cctgctggtc taacttggca
     3121 agcgttgtat agatctgact gtgatgggct tcccaaaaaa ttgtgatcag ctgcttcaat
     3181 tcatacccag aacgtggttt ggataaaagc acgcataata atatataggc taaagtattc
     3241 ataaaaatcc tacctctcgt cttattttct ttattatatc acagtaaagt ttccacgttt
     3301 tacatgtgca tattgacatg taaaataaat catgtatgat gctaatagag agaaagggat
     3361 ggaaatattg gagaatcaaa tagatataca agaacaaaca aatgaagaaa caaaggggaa
     3421 aagttcagcg ctagttgtag cagctacctg tatgatcgct tttatgggag tgggtctagt
     3481 agacccaatc ttaaaaacga ttgctgcaca gttaaac
//
LOCUS       DACDUU010000235         2768 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.guided.143, whole genome shotgun sequence.
ACCESSION   DACDUU010000235 DACDUU010000000
VERSION     DACDUU010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.guided.143"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..356
                     /locus_tag="IWL30_003005"
     CDS             <1..356
                     /locus_tag="IWL30_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="23S rRNA m2A2503 methyltransferase; methylates the
                     C2 position of the A2530 nucleotide in 23S rRNA; may be
                     involved in antibiotic resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP9707671.1"
                     /translation="PIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQQLADLL
                     KDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHGTDIDAA
                     CGQLRSKQMKKTVAK"
     gene            complement(549..1652)
                     /locus_tag="IWL30_003006"
     CDS             complement(549..1652)
                     /locus_tag="IWL30_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9707672.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            2205..2753
                     /gene="aac(6')"
                     /locus_tag="IWL30_003007"
     CDS             2205..2753
                     /gene="aac(6')"
                     /locus_tag="IWL30_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP9707673.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          906 a          480 c          538 g          844 t
ORIGIN      
        1 tcccaattga gaaattgatg gaagctgtgg atgagtattt agagaaaaca aaccgtcgaa
       61 tcacgtttga atatatcatg ttgaatcaag taaatgatag accagagcat gcgcaacaat
      121 tagcagatct attaaaagat aagaaaaaat tagcatatgt caacttgatt ccatataatc
      181 cagtcagtga acatgatcaa tattctcgca gtccaaaagc tgatgtattg agattttatg
      241 atgtattgaa gaaaaacggc gtaaactgtg tgatacgtaa agaacatggt acagatattg
      301 atgcagcttg cggacaattg cgaagcaagc aaatgaaaaa aacagttgcc aaataatcgt
      361 atcctactcg ttttttcaat taccataaaa ggagcagttt tcttaattaa ggaactgttc
      421 aacttaaata agagaaaaga ttctatagcg aaagctataa agcttttttt gacaagaaaa
      481 accctcatca agcaaagaaa tgtcctcaat ttgatgacag cctgcttgat gggggtttta
      541 gcaattattt aactgatttt cttatatcct tgctggcttt cttgtgtagc atgataagtt
      601 tctacgtcaa gaatggcctg tttaaatttt tctcgggtga tttcataagg cacatgttta
      661 atatcatttg ttgccatggc tttatctaag attgcttcta aatcggtcgt gacattgaga
      721 ttcatgtcgg ttaacgatac tggaagttgg ttctttttca taaacacata gattttatca
      781 aacaaatcga actgctgatc catcatcaat aaggtcagta caccataact gacagatgct
      841 ccgtgaaggt atccctccat tttttctaga acagtcgtac cgtaatagaa tgagtgtgcc
      901 atagcagaat tatagttatt gttaactaaa acagaaacca atccggtagt aacaatgata
      961 tccgaaacga cttgttcaat tgcatatgag ggacgatcgt cggcactatc tttcaatgct
     1021 tgcgtaccat aagtcaagag acgatcaatg cacatttttc cgatttggat tcctaatcta
     1081 tccgtgtagc ctagatcatc gccacgagca gaaaaagtag attcgacttc tttggataaa
     1141 gcatcgccaa tacctgccca taagtattta tcaggtgctt cggcaataat tgctgtatca
     1201 atgaaggtgt gtacaggtgg ttttgaagga aagaagagtc cgtccattac gccttcttcg
     1261 ttatagatga cacataccgc agtgacgggc gaacagttcg aaccaatagt gggaaaggtg
     1321 aagactggtt tagctagttt ttcaccaatg acttttacag tatcgcaagc acggccacca
     1381 ccgactgcaa acaggacgtc tgctttttgg acactttcat gctggatcaa ctgttcaata
     1441 tttccataag ttgaattacc cccgtaccag agactggcga caacctctat ttcctgtgtc
     1501 aaagctactt ttattttgtt ttctgccttt gaaagtgcag tcttccctcc aatcagcact
     1561 acttttgtcc catattcttt gacgattcca tttgcttttt catacgcatc tgttccaatg
     1621 gtataatttg gtaagaaaac actttgactc atattcattt ccttctttct tcgttgaaac
     1681 gggttaaaat tttaaagagt gatcgatctg actgcggctg tagatggagt agaggtgaac
     1741 ctaagacagg ataagtgatg aataaggtgg catgaacacc ttgaaatcat caataagtat
     1801 cctaaaaacg aacaagtaca aaaacattga aaacgacaat aatgaatttt tgttcctctt
     1861 agtatttcta ccgattccct ttgctttttt gtaattatct aaaaattcta aaaattaaga
     1921 ttattatatg aaaacaggtt ttatttgtca attcataatt ggtttttttt aaagcaaaag
     1981 ataatctata tcatgatgaa caaacagagt tttttcctcc aattgatggc tttcctgatc
     2041 tttcctattt tgaataaagt gtttctgtat aattttttta ttttataagt aagataaata
     2101 atgaaagaat agagatactg attggtagct aaagattttt gagcgagaac atttcgtttt
     2161 ttcaaaagga ctgttagaat aagcaagaac aagggggaag acgtatgata atcagtgaat
     2221 ttgaccgtaa taatccagta ttgaaagatc agctttctga tttactgaga ctgacttggc
     2281 cggaagaata tggagacagc tcggcagaag aagtagaaga aatgatgaat ccagaacgaa
     2341 tcgcggtagc agcggtagac caagatgagt tagtaggatt tattggtgca atccctcaat
     2401 acggtatcac aggttgggaa ttgcatccat tagttgtaga aagctcccga cgaaagaacc
     2461 aaataggtac tcgattagtc aattacttag aaaaagaagt agcttccaga ggaggaatca
     2521 cgatttattt aggtacggat gatttagacc atggaacaac gttaagtcaa acggacctgt
     2581 atgagcatac atttgataaa gtggcttcca tccagaacct tcgtgaacat ccgtatgaat
     2641 tctatgaaaa attaggttat aaaatcgtag gtgtcttacc aaatgcaaat ggctgggaca
     2701 aaccggatat ttggatggca aaaacgatta ttcctcgacc aaattctcaa taataaaaag
     2761 aggttgtg
//
LOCUS       DACDUU010000236         2459 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.guided.149, whole genome shotgun sequence.
ACCESSION   DACDUU010000236 DACDUU010000000
VERSION     DACDUU010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.guided.149"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..688
                     /locus_tag="IWL30_003008"
     CDS             <1..688
                     /locus_tag="IWL30_003008"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP9707674.1"
                     /translation="RYIKSPDYNGPDMTDFGHRCLTDPEFNEEYKKQSKLFCEKILTD
                     DSFAETYGDLGHIYGYQWRHWETKDGGFIDQIKQVIEAIKKTPDSRRLIVSAWNPEDV
                     PSMALPPCHTMFQFYVQEGRLSCQLYQRSGDVFLGVPFNIASYALLTHLIAHETGLAV
                     GEFVHTLGDAHLYINHIEQMKEQLSREIRAFPTLVLNQEKQSVFDFEMEDIQLEGYDP
                     HPAIKAPIAV"
     gene            708..1235
                     /locus_tag="IWL30_003009"
     CDS             708..1235
                     /locus_tag="IWL30_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9707675.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(1298..1951)
                     /locus_tag="IWL30_003010"
     CDS             complement(1298..1951)
                     /locus_tag="IWL30_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP9707676.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            2083..>2459
                     /locus_tag="IWL30_003011"
     CDS             2083..>2459
                     /locus_tag="IWL30_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family EDD domain-containing protein"
                     /protein_id="HAP9707677.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSF"
BASE COUNT          859 a          462 c          497 g          641 t
ORIGIN      
        1 acgctacatc aaaagtccag actataatgg accagatatg actgattttg gccatagatg
       61 tttgacagat cctgaattca atgaagaata taaaaagcag agcaagcttt tttgcgagaa
      121 gatcctaacc gatgattcct ttgctgaaac atacggcgat cttggacaca tctacggcta
      181 tcaatggcgt cactgggaaa caaaagacgg tggctttatc gatcaaatca aacaagtgat
      241 cgaagcaatc aaaaagactc ctgattctag aagattgatc gtttctgcat ggaatccgga
      301 agatgttcct agcatggctt tgcctccttg tcatacgatg tttcaatttt atgttcaaga
      361 gggacggcta agctgccaat tgtatcaacg cagtggggat gtgtttctag gcgttccgtt
      421 caacattgca agttatgcgt tgctgactca cctaattgca catgaaacag gtttagcggt
      481 tggcgaattt gttcacacat taggagatgc tcatctttat atcaatcata tcgaacaaat
      541 gaaagaacaa ttgagccgag aaatacgcgc atttccgact ttagttttga atcaggaaaa
      601 gcagtcagta tttgattttg agatggaaga tatccaatta gaaggatatg acccacatcc
      661 ggcgatcaaa gctcctatcg ctgtttagaa tcagaaagga tgagaaaatg ttagctgcaa
      721 tttgggcaca ggatgaaaac ggattgattg gaaaagaaga ccgtcttcct tggcgtttgc
      781 caaatgatct gaaattcttc aaacaaatga cagaagcaaa tacactagtg atgggacgaa
      841 aaacattcga agggatgggt agccgtccgt tgccgaatcg tcagacgatt gttttgacga
      901 gagattcttc ctataaagca gaaggcgtgc atgtcatgca tagtgtggaa gacgtgcttg
      961 ctctggaaaa agaaacagac ggtatctttt tcatcgctgg agggtcagct gtttatcaag
     1021 aatttctgcc attttgtacg atattatacc gtacagtcat ccatcatgta ttcgatggag
     1081 atgcttattt tccacctgtt gactggtctg attggtcact gatcaatact agtcaaggag
     1141 aaatggatga aaaaaatcgt tatccgcatc aatttgaaac gtatcagcgt aatgagaatc
     1201 ctgtaaaaag cgggacaggc gaattgacga tataaaaaag ctatggatag actagctgtt
     1261 atttagagct gtcccatcca tagctttatt ttttttatta agtaaagaat aaaacagaga
     1321 agtacataaa acctgcacct aacataacaa acaaatgcca aacaacatgc atatagcgga
     1381 tagaattcaa gctataaaag aatgctccta tcgtgtaaga aagcccgcct gctaccatca
     1441 aaataacacc agtcattcca agagagtgat aaagaggaac agcagccacg acacagagcc
     1501 accccataaa aatatagatg atcgtagata cttttgatac tctctcccgt ttatgcaacg
     1561 tcaacgcttt atatataatg ccaagaatcg caaacaacca gattaataca aataaagtcc
     1621 aacctagcca tcctcggata ctcagcaaac aaaaaggagt ataacttcct gcaatcaaaa
     1681 gaaaaatcga actatgatcg aatacttgga acaccttttt tgcttttgta aaaatcaatg
     1741 aatgaaacaa agtagacgtt aaaaataaaa gaatcatcat cgatccatag atagcataag
     1801 agacaatatg gagggaggaa tgcaagtggg caccttttac taaaagaacc actagtcccg
     1861 cgatactcaa tccagtccca attccatggg tgactgcatt aaacacttca tttaatattt
     1921 catacgttcg attaaatttc gttttttcca tgaaaacggc tcctttacta ggtgtaatta
     1981 atgaaaaatc attatttgtc tgttatacta aaaatagaaa ttgtcttaca tgtagtttaa
     2041 catgaataag gcatcttgtt aaagaaagag agagagaaaa taatgacaaa tgtaaaaatc
     2101 gtaacagatt cttcatgtac gatggaaaaa aatgtacgcg atgctttagg cattcgtgtc
     2161 atgccgctat ccatcatgat cgacggaaca gtatatcccg atgatgacca actagacggc
     2221 gaaagattta tggaaatgat ggcgaaagca aaaaatctgc ccaaaacaag ccaaccacca
     2281 attggagaat tcatctcgat gtatgatgag ctaggagaag acggcagtga agtgatttcg
     2341 atccatatga caaaaggctt gagtggaaca gtagaagcag caagacaagc cagtaatcta
     2401 acaaaaacga aagtaacggt tatcgacagt gataccactg accaaggatt atcttttca
//
LOCUS       DACDUU010000237         1795 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.guided.168, whole genome shotgun sequence.
ACCESSION   DACDUU010000237 DACDUU010000000
VERSION     DACDUU010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.guided.168"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            107..1609
                     /gene="lsa"
                     /locus_tag="IWL30_003012"
     CDS             107..1609
                     /gene="lsa"
                     /locus_tag="IWL30_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP9707678.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            1676..>1795
                     /locus_tag="IWL30_003013"
     CDS             1676..>1795
                     /locus_tag="IWL30_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9707679.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQALAEK"
BASE COUNT          638 a          315 c          398 g          444 t
ORIGIN      
        1 gcttggttct ttcgatgtat gggtcttttt gtgtgcccca acccttgcat cctctttagc
       61 cagttctgaa aataggactg gtcttttttt acgaggtgat ggaataatgt ctaaaatcga
      121 aataaaaaat ctgacattcg gctatgacag ccaaggcaca ttattatttg aacaagccaa
      181 tctaaacttt gacacacaat ggaaactagg acttatcgga cgaaacggtc gaggaaagac
      241 aactttactg aatatcctgc aaaacaaact cccttatcaa gggcaagtga tccatcagca
      301 agcgtttgtc tatttcccgc agcaaacaaa agataaagag cgtttgacct attatgtgtt
      361 aaatgagatt atggattttg agatatggga aattgaaaga gagctccaac tgatgcaaac
      421 agatcctgaa atcttatgga gagaattcag cacactatca ggaggagaga agacaaaagt
      481 cctactggca ctcttatttg tggatgacac tcatttcccg ttaatcgatg aaccaacgaa
      541 tcatttggat atctctggca gaaaacaagt agcggcttac ttgaaaaaga aaaaacaagg
      601 attcatcgtg gtgagtcatg atcggggatt tatcgatgaa gtagtggacc atgttttagc
      661 aatcgaaaag agccaactgg aactttatca agggaatttc tctatctatg aagaacagaa
      721 aaaacttcgt gatgaatttg aaatagctaa aaatgaaaaa ttgaaaaaag aagtcagcag
      781 gctaaagaaa acagcagctg aaaaagcaga atggtctcgt tcccgagaag gggacaaaac
      841 aaagaaaaaa gtcggattca tcgatacgga atctaggcga gtgaataaag gagcagtagg
      901 cgctgatgct gcacggacga tgaaacgatc taaagcaatc gtgaatcgga tggaaaccca
      961 gatcagtgag aaagaaaaat tattaaaaga tatcgagtat atcgatgctt tgacgatgaa
     1021 tagccaagtg tctcatcata aacggctgtt aagcgtagaa aatcttcaat tagggtatga
     1081 aaatctgtta ttcgagccag ttcattttac gatcgaacct catcagcgga tggcaatttc
     1141 aggtcctaac ggcgcgggaa agtcatccat tatccattat cttctgggga cattcaacgg
     1201 caaggttgta ggagaaaaat cccagcccaa acatttgacc attagttacg tacgccaaaa
     1261 ttatgaagac aatcgaggga cattggcgga attcgcgaag aaaaaccgag tagactatca
     1321 agcatttttg aataatctcc gaaagcttgg gatggaaaga gatgtttttc ataataaaat
     1381 cgaacagatg agtatgggac aacggaaaaa agtggaattg gctaaatctt tatcccaacc
     1441 agctgagctc tatatatggg atgaaccatt gaattatttg gatgtcttca atcaagaaca
     1501 attagaacaa ctgattttga acgtgaaacc tgctatgtta ctagtggaac atgatcaaac
     1561 ctttctagat aaagtatcta ctgagattat ttctcttgag aaaatctaaa ccttgtaatc
     1621 tcaaagacaa caaggtatgg ttaaggagaa ctttgtcaac gaaggagaga atcacatgct
     1681 ttatgtaacg agacacggag aaacaacttg gaacgcacaa ggattagttt gcgggcgagc
     1741 agatgtgccg ctgactgaaa aaggacagat gcaggcacag gcactagcag aaaag
//
LOCUS       DACDUU010000238         1782 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.guided.170, whole genome shotgun sequence.
ACCESSION   DACDUU010000238 DACDUU010000000
VERSION     DACDUU010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.guided.170"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            1..576
                     /locus_tag="IWL30_003014"
                     /pseudo
     CDS             1..576
                     /locus_tag="IWL30_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            609..1517
                     /locus_tag="IWL30_003015"
     CDS             609..1517
                     /locus_tag="IWL30_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAP9707680.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            1514..1762
                     /locus_tag="IWL30_003016"
     CDS             1514..1762
                     /locus_tag="IWL30_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin acetyltransferase"
                     /protein_id="HAP9707681.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLLTRGAS"
BASE COUNT          628 a          254 c          391 g          509 t
ORIGIN      
        1 gggattttat tctttattta aaactttgca acagaaccct ctgtagtagg tgttgatatt
       61 tctcataaaa tgctcgaagt agcaaaagga aaaacccatt ttccacagat tgaatatgaa
      121 tgctgtgcca tagaagatgt ggatttccca gaggagagct ttgatgtaat actaagttcg
      181 cttgcgtttc attatgtagc agactatgag aatttaataa aaaagatata taggatgctg
      241 aaggctggtg gcaatttagt ttttacagtt gaacatcctg tttttactgc tcatggaaca
      301 caagactggt attataacga aaaaggagaa atactgcatt tcccggtgga caattattat
      361 tatgagggca aacggacagc tatgtttttg gaagaaaagg ttacaaaata tcatagaaca
      421 ctgaccacat atctaaatac actgctttca aatagtttta taataaatca gattgtggag
      481 ccacagccgc cagagaacat gatggatatt ccggggatgg cggatgaaat gcgacgccca
      541 atgatgctga ttgtatcggc aaaaaagaag atgtaataat atagaaaaaa taaacgagga
      601 gtatgtaaat gagatcagaa aaagaaatga tggatttagt actttcttta gcagaacagg
      661 atgaacgtat tcgaattgtg acccttgagg ggtcacgcgc aaatattaat atacctaaag
      721 atgaatttca ggattatgat attacatatt ttgtaagtga tatagaaccg tttatatcta
      781 atgatgactg gcttaatcaa tttgggaata taataatgat gcaaaagccg gaggatatgg
      841 aattattccc acctgaagaa aagggatttt cctatcttat gctatttgat gattacaata
      901 aaattgatct taccttattg cccttggaag agttagataa ttacctaaag ggcgataaat
      961 taataaaggt tctaattgat aaagattgta gaattaaaag ggacatagtt ccgactgata
     1021 tagattatca tgtaagaaag ccaagcgcaa gggagtatga tgattgctgc aatgaatttt
     1081 ggaatgtaac accttatgtt attaaaggat tgtgccgtaa ggaaatttta tttgctattg
     1141 atcattttaa tcagattgtt cgccatgagc tgctgagaat gatatcatgg aaggtcggca
     1201 tcgaaacagg ctttaaatta agtgtaggca agaactataa gtttattgaa aggtatatat
     1261 ccgaggattt gtgggagaaa cttttgtcca cctaccggat ggattcctat gaaaacatat
     1321 gggaagcatt atttctatgc catcaattgt tcagggcggt atccggtgag gtggcggaaa
     1381 ggcttcatta tgcctatccg gagtatgata ggaatataac aaaatatacc agggacatgt
     1441 ataaaaaata cactggtaaa accggctgcc tggatagcac atatgccgct gatatagaag
     1501 agaggcggga acagtgatta cagaaatgaa agcagggcac ctgaaagata tcgataaacc
     1561 cagcgaacca tttgaggtga taggtaagat tataccgagg tatgaaaacg agaattggac
     1621 ctttacagaa ttactctatg aagcgccata tttaaaaagc taccaagacg aagaggatga
     1681 agaggatgag gaggcagatt gccttgaata tattgacaat actgataaga taatatatct
     1741 tttaacccgt ggtgctagtt aattttaata tcctaaagaa ta
//
LOCUS       DACDUU010000239         1541 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.guided.178, whole genome shotgun sequence.
ACCESSION   DACDUU010000239 DACDUU010000000
VERSION     DACDUU010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.guided.178"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..88
                     /locus_tag="IWL30_003017"
     CDS             <1..88
                     /locus_tag="IWL30_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP9707682.1"
                     /translation="AMHFTTITTTKEEQEEGLDFFFFGNKLN"
     gene            119..361
                     /locus_tag="IWL30_003018"
     CDS             119..361
                     /locus_tag="IWL30_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707683.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGVSQTKRY"
     gene            756..1229
                     /gene="dfrF"
                     /locus_tag="IWL30_003019"
     CDS             756..1229
                     /gene="dfrF"
                     /locus_tag="IWL30_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAP9707684.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            1356..>1541
                     /locus_tag="IWL30_003020"
     CDS             1356..>1541
                     /locus_tag="IWL30_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAP9707685.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADG"
BASE COUNT          553 a          202 c          321 g          465 t
ORIGIN      
        1 ggcgatgcac tttacaacga tcactactac aaaagaagaa caagaagaag gattggactt
       61 tttcttcttt ggcaacaaac tcaattgaaa caaattcaaa tagaaaagag gaagattcat
      121 gcaacaaagc aaaacgaaaa ttcctgcaaa aaaattaacg ttctttggct ttttggctat
      181 gaccatctca atggtcgttt cattatatga atatcccaca ttcgcgacct caggcttcag
      241 cttagtattt ttcttgttat taggcggttt gctatggttt atccctgtgg cattgtgcgc
      301 agcagagatg gcaacggtga aaggatggga aaaaggtgtt tcccaaacaa aaagatatta
      361 agaaacagcc catgtttacc tgcttgcaaa caaaagaata ttgctactag actaagagat
      421 actacataag tgtgttggta tctctttttt gttgccgtga aacagcagac tatttcattt
      481 ctgcttgatt accttaaaac ttattagtct atggtttgtc aagggcttgt tgcgtgagaa
      541 atgctgaata tcacccttta caaaacatag gctgataagt aacttttcaa aagcagaaaa
      601 gaaatggcaa tgcttataac aatatgatat aatgcagatg aagacaaaaa aggaatttgt
      661 cgagctggtt agttcgagat aattttgagt ttgttaagat gtggtgcact tgatggaatc
      721 acaggtacga catcatatta tggaggatga aatagatgat aggtttgatt gttgcgaggt
      781 caaagaataa tgttataggc aagaatggta atataccatg gaaaataaag ggagaacaaa
      841 agcaatttag agagttaaca acgggtaatg tggttattat ggggcgaaag tcttatgaag
      901 aaatcggtca tccgttgcct aatagaatga atattgttgt ttccaccaca acagagtatc
      961 aaggagataa tttagtttca gttaaatcat tagaagatgc attattattg gctaaaggac
     1021 gagatgtata catatctggt ggatatggac tatttaagga agctttgcaa atagtagata
     1081 aaatgtatat cacagaagta gatttaaata ttgaagatgg agatacattc tttccagaat
     1141 ttgatatcaa tgattttgaa gttttgatag gggaaacact tggtgaggaa gtgaaatata
     1201 cgagaacatt ttatgtaagg aaaaaatgaa ttgagtaaat tttggattta gggtgttttc
     1261 ataaatatat tgctttttat gcatatataa attgttgtaa gacaaattcc agtttgtatg
     1321 gggcgataaa atcagaattt gagagaggat tgaatatgaa taaggaatgg tctgaactta
     1381 ataaaacaat gcaggcacaa ataaaaaaga aagatactta taagaggggt attgatactt
     1441 tacttacttt acgaagtcag ttaatacaaa ccttagtatc tttcaaagaa gaattatgca
     1501 gagaagattt taactcaatc cccttcataa acgctgatgg t
//
LOCUS       DACDUU010000240         1349 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.guided.184, whole genome shotgun sequence.
ACCESSION   DACDUU010000240 DACDUU010000000
VERSION     DACDUU010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.guided.184"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            46..297
                     /locus_tag="IWL30_003021"
                     /pseudo
     CDS             46..297
                     /locus_tag="IWL30_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            390..1184
                     /locus_tag="IWL30_003022"
                     /note="aph(3')-IIIa"
     CDS             390..1184
                     /locus_tag="IWL30_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP9707686.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          424 a          234 c          322 g          369 t
ORIGIN      
        1 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
       61 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      121 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      181 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      241 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      301 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      361 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      421 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      481 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      541 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      601 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      661 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      721 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      781 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      841 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      901 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
      961 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1021 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1081 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1141 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1201 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1261 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1321 cgcctacgag gaatttgtat cgataagaa
//
LOCUS       DACDUU010000241         1028 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.guided.202, whole genome shotgun sequence.
ACCESSION   DACDUU010000241 DACDUU010000000
VERSION     DACDUU010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.guided.202"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            141..878
                     /gene="erm(B)"
                     /locus_tag="IWL30_003023"
     CDS             141..878
                     /gene="erm(B)"
                     /locus_tag="IWL30_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP9707687.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          391 a          172 c          153 g          312 t
ORIGIN      
        1 ttacgcagat aaataaatac gttagattaa ttcctaccag tgactaatct tatgactttt
       61 taaacagata actaaaatta caaacaaatc gtttaacttc tgtatttgtt tatagatgta
      121 atcacttcag gagtgataac atgaacaaaa atataaaata ttctcaaaac tttttaacga
      181 gtgaaaaagt actcaaccaa ataataaaac aattgaattt aaaagaaacc gataccgttt
      241 acgaaattgg aacaggtaaa gggcatttaa cgacgaaact ggctaaaata agtaaacagg
      301 taacgtctat tgaattagac agtcatctat tcaacttatc gtcagaaaaa ttaaaactga
      361 atactcgtgt cactttaatt caccaagata ttctacagtt tcaattccct aacaaacaga
      421 ggtataaaat tgttgggaat attccttacc atttaagcac acaaattatt aaaaaagtgg
      481 tttttgaaag ccgtgcgtct gacatctatc tgattgttga agaaggattc tacaagcgta
      541 ccttggatat tcaccgaaca ctagggttgc tcttgcacac tcaagtctcg attcagcaat
      601 tgcttaagct gccagcggaa tgctttcatc ctaaaccaaa agtaaacagt gtcttaataa
      661 aacttacccg ccataccaca gatgttccag ataaatattg gaagctatat acgtactttg
      721 tttcaaaatg ggtcaatcga gaatatcgtc aactgtttac taaaaatcag tttcatcaag
      781 caatgaaaca cgccaaagta aacaatttaa gtaccgttac ttatgagcaa gtattgtcta
      841 tttttaatag ttatctatta tttaacggga ggaaataatt ctatgagtcg cttttgtaaa
      901 tttggaaagt tacacgttac taaagggaat gtagataaat tattaggtat agggcacctc
      961 taataactac caattaattc acctcaaaat gaaattagaa aaaaacagcg aaatttttaa
     1021 atctattt
//
LOCUS       DACDUU010000242          891 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E31922_2015
            SAMN12639494-rid9882243.guided.211, whole genome shotgun sequence.
ACCESSION   DACDUU010000242 DACDUU010000000
VERSION     DACDUU010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639494
            Sequence Read Archive: SRR10030689
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741994.1
            Genome Coverage       :: 41x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:06:11
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,025
            CDSs (total)                      :: 2,965
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 32 of 81
            Pseudo Genes (incomplete)         :: 39 of 81
            Pseudo Genes (internal stop)      :: 23 of 81
            Pseudo Genes (multiple problems)  :: 11 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639494-rid9882243.guided.211"
                     /isolate="E31922_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..461
                     /locus_tag="IWL30_003024"
     CDS             <1..461
                     /locus_tag="IWL30_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HAP9707688.1"
                     /translation="VLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLC
                     HNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMY
                     GNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
     gene            complement(591..>891)
                     /locus_tag="IWL30_003025"
     CDS             complement(591..>891)
                     /locus_tag="IWL30_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9707689.1"
                     /translation="LFDSRFKTLQQNQPKYSKACASLDDGFEDAFQYTVQGNSHNRLK
                     STNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          334 a           86 c          161 g          310 t
ORIGIN      
        1 atgtattaga agagtatata ttgttgcgtg aaactattta taatgattta actgatatag
       61 aaaaagatta tatagaaagt tttatggaaa gactaaatgc aacaacagtt tttgagggta
      121 aaaagtgttt atgccataat gattttagtt gtaatcatct attgttagat ggcaataata
      181 gattaactgg aataattgat tttggagatt ctggaattat agatgaatat tgtgatttta
      241 tatacttact tgaagatagt gaagaagaaa taggaacaaa ttttggagaa gatatattaa
      301 gaatgtatgg aaatatagat attgagaaag caaaagaata tcaagatata gttgaagaat
      361 attatcctat tgaaactatt gtttatggaa ttaaaaatat taaacaggaa tttatcgaaa
      421 atggtagaaa agaaatttat aaaaggactt ataaagattg attatataat atatgaaaag
      481 ctattataaa agacattagt attaaatagt ttaaaaaaat gaaaaataat aaaggaagtg
      541 agtcaattcc agactcctgt gtaaaatgct atacaatgtt tttaccattt ctacttatca
      601 aaattgatgt attttcttga agaataaatc cattcatcat gtaggtccat aagaacggct
      661 ccaattaagc gattggctga tgtttgattg gggaagatgc gaataatctt ttctcttctg
      721 cgtacttctt gattcagtcg ttcaattaga ttggtactct ttagtcgatt gtgggaattt
      781 ccttgtacgg tatattgaaa ggcgtcttcg aatccatcat ccaatgatgc gcaagctttt
      841 gaatattttg gttggttctg ttgcaaagtt ttaaatctac tatcaaataa g
//
